LOCUS       DAORCQ010000001        47737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_340_length_47737_cov_5.181914, whole genome shotgun sequence.
ACCESSION   DAORCQ010000001 DAORCQ010000000
VERSION     DAORCQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 47737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47737
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_340_length_47737_cov_5.181914"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PKI23_00005"
     CDS             complement(<1..1054)
                     /locus_tag="PKI23_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNN35350.1"
                     /translation="MTAVKKAYNHMYGKDPYSIPLEDYDVSQPALFESNTMWGFFDRL
                     RKEEPVHYCKDSENGPFWSVTKFNDIMTVEGNPQIYSSEPSIGIADPREELPLKMFIA
                     MDEPKHGVQRKTVQGIVAPSSLAKMEGLIRQRIAEILDTLPVNETFNWVDRVSIELTT
                     QMLATLFDFPFEQRRKLTYWSDVATAVPTPGGLIESDEERESILVNECMATFVGLWNE
                     RVNSPPKHDLLSMLAHNEETKHMQPMEFLGNLILLIVGGNDTTRNSLSGGVLALNQFP
                     EEYQKLRNNPGLIPRMVPEIIRWQTPLTFMRRTATCDTVLRDKQIKKGDKVVMWYVSG
                     NRDEEVIDRPYEFIIDR"
     gene            complement(1197..3401)
                     /locus_tag="PKI23_00010"
     CDS             complement(1197..3401)
                     /locus_tag="PKI23_00010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNN35351.1"
                     /translation="MHSEETLYLQVALPVPLRRTFDYLAPSAEPMAVGTRVRVRFSGR
                     LLVGVVVAIAARSSVAAGKIRPVETVLDEHPLLPQPLLQLALWSAEYYQQPIGELLAA
                     TLPPPLRHGQPLRKVMERHYRLSPAGATLHETKATGRSRKQQEILARLTRDSGLDHAE
                     LRALGLTGAPLTALLKKGLLRCDWRLPSSHAPSASSTAETGPPLNPHQQAACTAMRQR
                     WGAFSVTLIEGVTGSGKTEIYLQLIDDVLARGQQALVLVPEIGLTPQLQQRFAQRFPQ
                     ARIAVLHSGRTAGLRMIDWLHSAQGTADIILGTRSAIFVPMARPALLIVDEEHDSSYH
                     QEERVHYCARDLAVRRGQIEQVPVLLGSATPSLESLANVERGRYHHLSLPERAGNATL
                     PPIRLIDLRGRPLQQGFSLPLLALIDQHLTAGNQVLLFHNRRGYATALLCHDCGWMAT
                     CPLCEVRLVLHRHRPGLRCPRCDQRRTPPKACPQCQSQTLIALGSGTERCEETLAQRF
                     PDVPLIRIDRDTAHNSATLTELLEQVERQAPAILVGTQMLAKGHHFPRVTLCAILDAD
                     GAFFSSNFRAMEQMAQLVTQVAGRAGRAERPGEVAIQTHHPDHPQLLELIEADYGHFA
                     AAELQRRAMATLPPYRHLALILADAPAPEAAQRFLQGLTPPPPHSGIEWLGPNPAPID
                     RRGGRHHAQILLIARNRPHLQQLLRRLASELERLPTPAGLHWNIAVDPLESL"
     gene            3678..3902
                     /gene="rpmE"
                     /locus_tag="PKI23_00015"
     CDS             3678..3902
                     /gene="rpmE"
                     /locus_tag="PKI23_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNN35352.1"
                     /translation="MKSDIHPKYTKMVASCRCGATIETRSTLGRDMNIEVCSNCHPFY
                     TGKQKVLDTGGRVERFRKRFGRPSVAASTE"
     gene            4169..5452
                     /locus_tag="PKI23_00020"
     CDS             4169..5452
                     /locus_tag="PKI23_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNN35353.1"
                     /translation="MSDSLKQTALECHELPRPGKLEISVTKPTGTARDLALAYSPGVA
                     EPVREIANDPENAYRYTAKGNLVGVISNGSAILGLGNLGPLASKPVMEGKGVLFKRFA
                     GIDVFDIEVDAETPQAFIDTVVRIAETFGGINLEDIKAPECFEIERVLIEQCAIPVFH
                     DDQHGTAIVTAAGLLNALELQGKRLEEATIVCLGAGAAAIAGMRLLISLGADPERIFM
                     LDRKGVIHADRDDLNQYKAAFARETDKRSLADACAGADVFIGLSGPNLFPAQALKMMA
                     PRPVVFACSNPDPEISWQMAHAARDDLIMATGRSDYPNQVNNVLGFPFIFRGALDVRA
                     RVINEPMKIAAVHALRELAKAPVPQEVMDAYEGRRLSFGPEYIIPKPMDPRLLGQVSA
                     AVARAAVDSGVAQKSYPAHYPLTSTDEVFGGGRTG"
     gene            complement(5489..7930)
                     /locus_tag="PKI23_00025"
     CDS             complement(5489..7930)
                     /locus_tag="PKI23_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNN35354.1"
                     /translation="MPVTPPLFTPRPLFMLQLIRITLATLCLFTLALFGLYLYTVPRL
                     PDVELLREVELETPLLIYSKEGLLIGEFGEQRRSPLTYEEIPQRFIQAILAAEDDQFF
                     SHPGINPGSLLRAALELIQSGRIKSGGSTITMQVAKNYFLSQERTFDRKLKEILLAIK
                     IERELSKQEILALYVNKIYLGNRAYGIQAAAEVYYGKKIDALDLSEIAMIAALPKAPS
                     RFNPIADPERAGLRRNWILGRMLSLGYIDQAEHDAAITVPPATRYHGPQRDLRAEHVA
                     EMARQEILARYGSKAYSSGLQVITTLDARLQQSANQATLSGVLKYDRRHGYRGSEGQI
                     DLKEVTSRDARLARIMALPAFSGLQRALITRIDGKQIDILLENGEELSLPWDKLLGWS
                     GRKISQVALPRNPKPGKHPFSPGDLIRLFREQDGSWILAQPPQVQTALLCIDANSGAI
                     QALVGGLNAESNHFNRVTQGERQPGSSFKPFIYSAALENGFTAASIIDDAPLVFQTSP
                     FEPEWRPENAGGDFSGPIRLRQALYLSKNLVSIRLLQAIGPDKAISYAQQFGFAKERL
                     PRDLTLALGTGTVTPLEMTRGYTVLANGGFKVEPYLIEAVYDRYGQELYRANPARVCH
                     ECDTENPAPEHPAAIDGSAASKSAPRVMDARTQYLINDMLKDVITKGTGRAALALNRH
                     DIAGKTGTTNEGKDAWFAGFNPNLVTTVWVGYDQPRSLGEYEFGGTAALPIWIDFMRT
                     ALDGVDEVQLPQPPGIVAVRIDPVTGERAGPDMSHAIFELFKAEELPQQETRSPPSSS
                     PEGAAPSPTDSRELF"
     gene            8158..9216
                     /locus_tag="PKI23_00030"
     CDS             8158..9216
                     /locus_tag="PKI23_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNN35355.1"
                     /translation="MFGLFKKKQTPLLGIDISTTAVKLIELSCSSDSYRVESYAVEPL
                     PPSAVVEKSIADVEAVGQAIGRVVSRAKTKARDGVVAVAGSAVITKTIEMDADLTDDE
                     RETQISLEADQYIPYPLEEVAIDFEVQGPSESDARKVQVLLAACRHETVEAREAVLAI
                     AGLTPRIVDVEAYAIERAFLLVKDQIDPDARVVAIVDIGATMTTLSVLVEGKTIYTRE
                     QLFGGKQLTDEIRRRYGLSEEEAGLGKKQGGLPDEYEDEVLAPFREAAVQQVARSLQF
                     FYASSQHNDIDCLILAGGTSSISGMTELLQQRIGVPTFLANPFLGMTIAPKVNAAALT
                     NDAPALMIACGLALRSFD"
     gene            9216..9782
                     /locus_tag="PKI23_00035"
     CDS             9216..9782
                     /locus_tag="PKI23_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNN35356.1"
                     /translation="MAKINLLPWREALRAERKKRYMIALTVTALVAVLLVLGGSRYIQ
                     GEIENQQARNQFMQREIAQLDAQIKEILGLRKRRDELIDRMKVIQSLQGNRPVIVRLV
                     DELVRTLPDGVYYTSLDKQGEQITIQGVAKSNNRVSALMRNFDRSEWFANPNLLGVKA
                     FPEFGEQANSFNLLVQQKNPDAGEAKKQ"
     gene            9791..10420
                     /gene="pilO"
                     /locus_tag="PKI23_00040"
     CDS             9791..10420
                     /gene="pilO"
                     /locus_tag="PKI23_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNN35357.1"
                     /translation="MAILDRIKNLGKIDFSKIDLNDLDLDNIGSWPMPIKVLLALLLF
                     AALLGSGYWFYLKEMELELETAARQEQQLKESFSAKSFQAANLEQYRQQMKEMEESFG
                     TLLQQLPKDTEVPGLLEDITHTGLGSGLEFQSIRLLPEVKREIYIELPIEIVVTGSYH
                     DLGSFVSGVAALPRIVTLHDFSLRPDSNSGLLTARIIAKTYRYKDGAAQ"
     gene            10417..10944
                     /locus_tag="PKI23_00045"
     CDS             10417..10944
                     /locus_tag="PKI23_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNN35358.1"
                     /translation="MIRASTRWGLVLLCLLLQGCGQEDPFADLKAYVEEVRAKPKGTI
                     EPLPKFKPYESFAYSAAGMRSPFDRPIEIQAVQRRMEGEKVAPDTNRAKEFLEGFNID
                     ALVMVGTIAKTDQIWALLSDGSGGIHRVTQGNHLGRNHGRVVAVHENKIDVVEIVPDG
                     QGGWVERPRTLDMKE"
     gene            10980..13124
                     /gene="pilQ"
                     /locus_tag="PKI23_00050"
     CDS             10980..13124
                     /gene="pilQ"
                     /locus_tag="PKI23_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010453646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNN35359.1"
                     /translation="MTLMRATKKALQDGRWIASRFVALWGLLFALGVQGAALQGVEFA
                     SLPNDRFEIRLDFDGAPPQPQSYSIEQPARIAIDLVGVKSALGKKSYSLDHRNARSVT
                     VVESKDRTRMIINLVDLAPYSTQVDGNTLRVVLGEKQAAAVTAATPAPVAAPVAAAPT
                     AVPVAAAPPPAAVAVTRPAVLPARGSQVEKVDFRRGESGEGKLVVTLSNSGANVDVRE
                     EGGKIRVEFSDTRLLPALRRRFDVTDFATPITSIDATEESGKAVMTVTPTGHYDYLAY
                     QADTEFTLSVKPLTAEEQADEQKKKFAYTGEKLSLNFQDIEVRAVLQLIADFTNLNLV
                     ASDTVTGRITLRLKNVPWDQALDLVLKTKGLDKRLVGNVLTVAPAAEIAAREKLELET
                     NKQVNELAPLHTDFIEVRYAKAADLLAMLTGKNSILSTRGTASIDKRTNLLIVQDTDQ
                     VLAEVRSLLKRLDVPVRQVLIEARIVKARANVSKDLGIQWGGRYGDNDGNNMVIGGSG
                     VRAGVPGPVTGGDVVNLGVTKTGTTTLNLGFVNNSNILNLSLTAIEENGDSQTLAQPK
                     VLTADKQMAKIEQGTEIPYQEATSSGATSTSFKPAVLALEVTPQITPDNRIIMDLQVN
                     QDVPGAVTNGIPSIDTTRIKTQVLVDNGQTVVLGGIYTQDDTFTETKTPLLGDVPYVG
                     SLFKRTSKGKIKSELLIFITPRLDGEGKSSLQ"
     gene            13121..13660
                     /gene="aroK"
                     /locus_tag="PKI23_00055"
     CDS             13121..13660
                     /gene="aroK"
                     /locus_tag="PKI23_00055"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HNN35360.1"
                     /translation="MSRADRNIFLIGPMGAGKSTIGRLLAERLGLPFKDSDHEIEARS
                     GADIPWIFDVEGEAGFRRRETAVLADLTCEQGIVLATGGGAILKEENRTLPKQGGTVV
                     YLAASVEQQYARTRRDRQRPLLREADPHARLAQLFAEREPLYLSIADCVVTTDRRTPR
                     LVVQEILRHLGRDDPTTAS"
     gene            13886..14902
                     /gene="aroB"
                     /locus_tag="PKI23_00060"
                     /pseudo
     CDS             13886..14902
                     /gene="aroB"
                     /locus_tag="PKI23_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302367.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
     gene            15079..16593
                     /locus_tag="PKI23_00065"
     CDS             15079..16593
                     /locus_tag="PKI23_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN35361.1"
                     /translation="MAGLRATDDAALEQALAAPSIPFFPGAGREQLLTQLEHWSRYGN
                     GILIVEGSEQSGKTTLAGELHQRFGNEMTLLSICASGQTQSIDLLIEIADALGIESGL
                     EPDEADLLEKIQQIAQEAQADASAVAIVIDDAHELDEASYHLLFRLAFNPEDNQPLIS
                     VILLSLPGIDLWRNVPEGMAWPTLAVLRPLDFEEALEFWRHCRQQSGGSADAIEDSRT
                     ARRLWADAEGLPGNLLRRFPDQKESAAAAVPSRAALFSLPNWHLLAIVAVGLLLAAVL
                     WFGNRESAPPEQQSLSIALPTEPAAPVVPLPAAPPSSEAQPAPTTLPPSMPPVSTPIA
                     SEQPAVVPAPVPAPLPKPKVIPAPVEPPKAVEKPKPAPVKEPAKASKPATPPKQAVAA
                     VPASKATLATPEVAQQHQKLLALAPERFTLQLLGASDLSKVRNYIDRELGCKDCYYYE
                     TRRDGKPWFTVVKGVYADRAAAVAARDKLPAKVRAQQPWPRSVRDIQKELRQQR"
     gene            16887..17099
                     /locus_tag="PKI23_00070"
     CDS             16887..17099
                     /locus_tag="PKI23_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNN35362.1"
                     /translation="MAWSEVAKIDINSADEATLSTLDGVGPAKAKAIIEYRNKNGKFK
                     SIEEFEKVPGIGPATLKQNAARLTVK"
     gene            17273..17974
                     /gene="rnt"
                     /locus_tag="PKI23_00075"
     CDS             17273..17974
                     /gene="rnt"
                     /locus_tag="PKI23_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017844623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HNN35363.1"
                     /translation="MNDSSTSAERSAGFWPIGNRFRGFLPVVVDVETGGFNARTDALL
                     EIAATLLRMDEEGYLHCEQTHFYRVAPFAGSNMEPAALAFNKIDPYHPFRLAKSEDEV
                     LTDLFQHVRKAIKANHCTKAILVGHNASFDHGFLNAAVARCRIKRNPFHPFSSFDTAT
                     LAGLAFGQTVLARACRCANLEFDNDQAHSARYDTFKTAELFCFIVNEWKRLGAKPPNS
                     EGEVDGEAVDDEVDD"
     gene            18140..18883
                     /locus_tag="PKI23_00080"
     CDS             18140..18883
                     /locus_tag="PKI23_00080"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNN35364.1"
                     /translation="MQALDAAEQRGLEIAREADRRDQGWGDSSTSMEMILRSKEGETA
                     SRALTVKVKEGQPAVEEGDRSLIVFNSPKDQKGTALLTYSYRTRSDEQWLYLPALKRV
                     KKIASDNKSGPFVGSEFAFEDFSDQEVEKYRYRYLRDEPFNGIDCFVMERYPLDSNSG
                     YSRELVWIDQAHYRIHKVDYYDRKQTLLKTLSVSGYQQYNGKYWRADLSEMVNHQNGR
                     STSLKAADYRFSQGLSDDEFSQNSLQRAR"
     gene            18886..20136
                     /locus_tag="PKI23_00085"
     CDS             18886..20136
                     /locus_tag="PKI23_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35365.1"
                     /translation="MKKLGSALLATLLWTGSASAYELDGELALEGRLFADRGPRGQEQ
                     LDGAIWTRWQYFNDWNDGKDSITVVPFLLQDQRDAERSHWDFRELTWLHVGDGWEFRG
                     GLRRVFWGVIESQHLVDIINQTDAVLAIDGEEKLGQPMLNISTEGRFGTLDLYLMSFF
                     RDRTFPGIDGRLGGPLVVADDSSRFEASDGRRHVDVAIRWAHNLENWNFALSHFSGTS
                     REPLFQLEYLGASPIPTGRLIPLYPTIDQTGLELQYIQGGWAWKLEAISRSGYGGDRY
                     AASGFGFEYTQTGIAGSRIDLGWIVEYDWDERGDQAPPGVLEHDLFLGTRWAFNNLGE
                     SQLLAGVVVDSDSREQVYWFEGSHRLSDQWKLAFESRIFAGGHLPPTDLTSILTQAAQ
                     PDFDNKLGYWQKDDFVQLQLIRYF"
     gene            20180..20659
                     /locus_tag="PKI23_00090"
     CDS             20180..20659
                     /locus_tag="PKI23_00090"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNN35366.1"
                     /translation="MFLRVSLSRQLLDLLDEHGGVQRSFAISSSARGAGELVNSQKTP
                     RGLHRIRAKIGTGVPVGGVFVGRRYTGEIYTPKLAARFPERDWILSRILWLCGCEPGR
                     NRFGNVDSARRYIYIHGTPDSEPMGVARSHGCIRMRNSELLWLYEQVAVGSRVLIEE"
     gene            20664..21722
                     /gene="nagZ"
                     /locus_tag="PKI23_00095"
     CDS             20664..21722
                     /gene="nagZ"
                     /locus_tag="PKI23_00095"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNN35367.1"
                     /translation="MPNSSDNHSAALGPVMLDVAGSVLSDRERERLRHPQVAGVILFS
                     KNHVNRSQLVELIDEIRSIRADLLLAVDQEGGRVQRFGSTLTDLPAAGWFGRHYLQQP
                     AETLLLLRESAFLTGHELAELGFDFSFAPVVDLDYGRSAVIGSRAYHRDPELVTVLAS
                     AWIDGMRQVGMAAVGKHFPGHGWVSADSHLELPEDERPLSDIIRQDLFPYARLIDQLD
                     AIMPAHVRYPAVDPLPAGFSAFWLRQLLRERLGFEGVIISDDLSMQGAAGMGDLIGRA
                     NAALEAGCELLLLCNCGDEVDPLLRHLEQQRFQGSTRLGRLRRAGIAAAAEQQQRVLA
                     AREALDRLRLQQRINNGA"
     gene            21748..22290
                     /locus_tag="PKI23_00100"
     CDS             21748..22290
                     /locus_tag="PKI23_00100"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009405927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNN35368.1"
                     /translation="MNAAERYEEILASAQCLYSTAEVEAAIVEMARQITARIGATNPV
                     VCSVLTGGIVLTGKLITQLPFALELDYLHLSRYQNRTEGGLLQWKARPTIDCTGRTVL
                     LVDDILDEGTTLQAAVEHFRQMGAVDVLTAVLINKRHQRKHGLQVADFVGLETEDRYL
                     FGYGLDYHGYFRNVPGIYGL"
     gene            22287..23252
                     /locus_tag="PKI23_00105"
     CDS             22287..23252
                     /locus_tag="PKI23_00105"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNN35369.1"
                     /translation="MIVDRQGRRFRKLRLSLTAACNYTCIYCVPHGKRLIKRADELES
                     AQFIRAVRLLQLATGIEAVRITGGEPLVSPQLEPVLQGIARLGLTDLSLTTNGQLLTD
                     RLPVLLAAGIRRINVSLDTLDPLAFRTISRAGDLPAVLAGIEAARAAGMAVRVNMVPM
                     RRRNHDQIVPLLDYCLERGIELRYIELMRMGHLLNSPDFAADFVSMDSLLDLIGKRHP
                     FQRTDAPHDSTAVRFEVPGRGTFGIIPNESEPFCRACTRLRLSSDGWIYGCLSNSRRH
                     RINPILEQPDHVALPALQRLLTDALADKQDQAFQGGVTIMKLVGG"
     gene            23365..24426
                     /locus_tag="PKI23_00110"
     CDS             23365..24426
                     /locus_tag="PKI23_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HNN35370.1"
                     /translation="MLALFCTVFSRHGVAVDLPDPYLARVEVADQSTQARDSALQRAL
                     HEVLIRLSGDQQVVALPAVKEVLSKADSLLDQYGYDRAPALAPDGPARLLLEARFRPE
                     AIMALLRAANALYWAPNRPQLLIWSVLEQNQERRLLNASEMQALLPALFTAAQQRGVP
                     LLFPLNDLEDATALPLDQLWLLNGEAITAASLRYHPDGVLAGRIEQRPDGRWQARWWF
                     DDNGERWQLDLDADALDALAAGAIDQVANNWASRHAVRLGASAEQLELEITEVNTLAR
                     YAAVMSHLQKLPWARRVTLVGVSGPMLRFSLVSDVDRSQSQQLLQLDGKLEQQSQPVA
                     DAPPGVVPTTALRYRWRGG"
     gene            24493..25044
                     /locus_tag="PKI23_00115"
     CDS             24493..25044
                     /locus_tag="PKI23_00115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNN35371.1"
                     /translation="MLRQLPNLITLMRIVLVLPVGVSVIREQYQQAFWLFALAGFSDG
                     VDGLLARLFDWRSRFGAIADPVADKLLLVTTFVAGAVSGVWPWWLMVVVLIRDLVIFS
                     GSVAYHLLVGPYQMAPTWLGKLCTFSQIGVVLLLLQGRFWLPLPPWSQSLMVAFVALV
                     SIVSGLHYVLVWSRKYREQRFGK"
     gene            25022..25741
                     /gene="hda"
                     /locus_tag="PKI23_00120"
     CDS             25022..25741
                     /gene="hda"
                     /locus_tag="PKI23_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNN35372.1"
                     /translation="MSNVLESEPRQLALGIAPRFEATFDHYLPGENGLILALLHEAVV
                     AEARSPRLVYLWGEPGCGRSHLLQASCHLAAELGRDFFYLPLTADLGAPPRSFVELAA
                     AELLCLDDIDQVAGDPQWEEALFHLLNRLRQNGHTLLATACNVPTGIGLKLPDLSSRL
                     AAGVTVQLQPLNEADRRRLMKQQAERKGLALSDEAVEFILRRSPRGLSDLTALLERLD
                     QQAWVEQRRLTIPFIKQTMNW"
     gene            complement(25787..27022)
                     /locus_tag="PKI23_00125"
     CDS             complement(25787..27022)
                     /locus_tag="PKI23_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN35373.1"
                     /translation="MDFKDSPEEAQFRAKARAFLDAHAERKKGPRDNWQRLVKSEAEA
                     VELAKKWQRTLHDNGWACLHWPKEYGGQDASAIERVIWDQEESQYLVPKGFFTIGLGM
                     AGPTLMTWASEEIKREHLPKMVKAEKIWCQLFSEPVAGSDLAGIRTRAERDGDDWIIN
                     GQKIWTSGAHYSDWGILVTRHDPSVAKHKGLTYFIVDMKSPGVEIRPVKQITGNSGFN
                     EVFFTNVRIPDSQRLGKIGQGWQVALTTLMNERVAVGERQGVDVAELLDLAKGVQING
                     RPAIEDGAVRAKLADWYCKSSGLKFTRFRSISAMSRGEVPGPENSIGKLVGAFKSQDL
                     AAFGMELMDMAGIITDSTIAPFDAQFQQTFMQSPSGRIAGGSDEVLRNIIAERVLGLP
                     QDVRTDKELPFNQIPSGKG"
     gene            complement(27051..28163)
                     /locus_tag="PKI23_00130"
     CDS             complement(27051..28163)
                     /locus_tag="PKI23_00130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN35374.1"
                     /translation="MNFEFSDDQKLLKQQANKFLTDRCTLRDVRKILESDQPFHRDLW
                     NQVASMGWTSTAIPEEFGGLGLGHLELCVVAEEVGRALAPIPFSSSVYVATEALLLAG
                     SQQQKEHYLPKLAAGELIGTFAVAEGPGNPDPASYSVSYSNGKLSGAKIPVIDGDIAD
                     FAVVIAKSGQGASLCLVDLTDPSVKRTTLKTLDPTRSQAHLVFNDTPATLLGQEGAGA
                     RLLDQVFNRAAVLYAFEQIGGAEQALHQAREYAMGRYAFGRPIASFQALKHKMADMYV
                     ALELARSNGYYGAWALSTNAPELPLAAATARVSATKAMYECARENIQIHGGMGFTWEF
                     DCHMFYRRAKLLSLVIGSQNRWEEKLIQALTAGQAA"
     gene            complement(28514..29623)
                     /locus_tag="PKI23_00135"
     CDS             complement(28514..29623)
                     /locus_tag="PKI23_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNN35375.1"
                     /translation="MSKTKNRTSKLAWVSAGAVCAATALATLPSHAADDRIYISPTIY
                     RAMLDSGRSYDSDYAGQIQIGKALSDSINVEGYLDYGKFDRESSTGKDLEQIGLGLDA
                     LYFFNRSSIAPYLLVGAGAVKSSGAYPAAPTVGQSSTDFAFNFGAGILWQVMDNGTAI
                     RTELRTRSVDDGLEGETSPVGDRGSRTRWDTFAGIGLTIPLGSKPAPAAEPVAEPTPP
                     PPPPVVDTDSDGDGVVDRLDQCPGTPAGAKVDSTGCLVAQVLLLKDVNFEFNSAKLTS
                     ESKIILDQAAAALKKTPGKKVEVAGHTDAVGSNTYNMKLSQARAKSVRDYLIAQGVDA
                     ARLTSAGYGEEKPVADNGSDEGRAKNRRVELHIKD"
     gene            complement(29759..30955)
                     /locus_tag="PKI23_00140"
     CDS             complement(29759..30955)
                     /locus_tag="PKI23_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35376.1"
                     /translation="MNRIQNLLRLPLLALLLMLFGCSAGHPNSCKGVLGTQPYLTIGR
                     DYDEGYYRTASESWLQAMLAANAYDPPGAGRFKLPETVSERAFFDPGRGLQAKLYAIG
                     AEQGQPHKLVIAFRGTTSLYDWLFGNLLNSQYALADTLVAAIRQQYPTVELIATGHSL
                     GGGLALHVSMVFDGVSAYAFNPSYKVLSPPIPKMNRRVVVGASGDILALQRRFWFQPE
                     VDVDHPAYNCTHVNQHDITLLSRCLLHVAAAGEQRAQLTLAQNRSPLCGSLGWISLGP
                     RRDQQRLIEQFRQTGSAAVTLAAEAYLRRDPPQPPFYRLAGAIRVIPAGTTLSVLDLI
                     DSVGWRQRSWIKVAYDPVETSSETNVESIETTQGEEGNLNSLAPPVSASESSAAESPT
                     DAVEQH"
     gene            complement(31054..31710)
                     /locus_tag="PKI23_00145"
     CDS             complement(31054..31710)
                     /locus_tag="PKI23_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HNN35377.1"
                     /translation="MLLSHRYNFLFVHIAKTGGTSVRDALDRLRWQDPKYYPMWLCSR
                     LSALFNHELACKLPRHAKAIAAQEMLPRELYQRLFKFAFVRNPWDLQVSSYHHLQRER
                     PQLLTHVTSFEEFIRFKLDPQRPYQYHLDTSIELQSDYLVDLHGRLIVDFIGRYERFD
                     ADFEQACQRIGITPPRLPHKRKATDRRDYRSYYSDTLAEQVGAHFRLDIERLHYRFDP
                     "
     gene            complement(31732..32865)
                     /locus_tag="PKI23_00150"
     CDS             complement(31732..32865)
                     /locus_tag="PKI23_00150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN35378.1"
                     /translation="MSQTDHDRPVALFLSYSGNGGVERMMTLLARGIAERGHPVDLLV
                     IKGHGEHFAVPAGVRVIHSGARHTWQAIPFLLCYLRRTPPRALLAAKDRANRVAILAR
                     RLAGRSDIPLVIRLGTHLEQSMKGKHPLRLWWRQRQMARSYRHADRVVAVSEGVAADT
                     RHLTGLTADRVRVIRNPTLFPEISERAAEPASHPWLDDPALPVIVGIGRLTRQKDFST
                     LIRAFARLRATHPSRLLIFGEGKDRPQLQALISELGLTEWVDLPGFTANPYAALARAR
                     LFVLSSAWEGSPNVLVEAMGLGTPVVATDCESGPREILAGGRHGPLVPVGDAERLAEA
                     MATVLDQPPPAAQLQQAVAEYSLALSTLRYLQALGVESPDATV"
     gene            complement(32862..34679)
                     /gene="asnB"
                     /locus_tag="PKI23_00155"
     CDS             complement(32862..34679)
                     /gene="asnB"
                     /locus_tag="PKI23_00155"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNN35379.1"
                     /translation="MCGIAGIIKREARVEQRELAAVAPLLRHRGPDDEGQYIKENLGL
                     LHTRLSIIDLGGGHQPLFDPRNDTALIANGEIYNYLELRAPLRAAGCHFSTASDCETL
                     LHSYACHGRDFLAPINGMFAFALHDGRRRSLYLGRDRLGIKPLYYAELPDRVIFASEL
                     KALLPLLPHAPAIHAPALLQFLQNQFNSGRDTIVEGIKRVLPGELIEIDARLQLHHHR
                     YWSPTTLTPRQIGFDEAREEFDQLFRQVMVEHMRSDVPFGLFLSGGVDSSVLLAMLTR
                     LRDEPPRTYSVGFSEGNGVDELDQASELARQFNTRHSALRLSGAELFARLPHTVWAAD
                     DLMRDYASLPTAHLAQAAGAELKVIFSGEGGDEVFAGYGRYRPKAAERWLKQLARPGS
                     GGFRTTGQWHARWSRRLFGAELKQAHAAMRAPFIAAWQRTPAHWSDLMRRQYVDLTTA
                     LPDNLLVKADRMLMAFGVEGRVPFLDHRIVEFGLMLPDSLKIDASGGKRFLKRWAESC
                     LPTELLQRKKRGFHVPVGEWLQGALLERMAERLPRNAAVREWFDPQGVSALIKARQQG
                     APVSRELFSLLQFALWHRLFIDAPGTLPAPAENTLEWLE"
     gene            34770..35816
                     /gene="waaC"
                     /locus_tag="PKI23_00160"
     CDS             34770..35816
                     /gene="waaC"
                     /locus_tag="PKI23_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HNN35380.1"
                     /translation="MSRRILIIRLSAIGDIVMASPLIPALRAAHPDGWLAWMVEEGME
                     SLIEAHPQLDEVIIWPRRRWQRLWREDRRNWWREVRAFVRRLRGSRFDLVLDAQGLLK
                     SAAWAWLSGAPRRIGLNSREGSRWLMQQVVHELDDHPGMSSEYRRLAQFLGLSTDDFH
                     PSIALTEADRARVLEMVGSERAARGYAVAAPFTTRPQKHWPQAHWRQWIDLLAEQTGL
                     PTLLLGGPGDRAAAQQISAGCRHAPIDLVGQLTLRQSAAAIASARLLVGVDTGLTHMG
                     AGFRVPTVALFGSTRPYLDAGLPGFRVIYHALACSPCRRHPTCAGRFDCLGGIAPAEV
                     WEAARQVMAEEAAR"
     gene            35813..36910
                     /locus_tag="PKI23_00165"
     CDS             35813..36910
                     /locus_tag="PKI23_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN35381.1"
                     /translation="MKVLHVETGMQIYGGARQVLYLLEGLQARGVDNLLVTPRGAAIA
                     EAARALGVPVRELPMRGDLDIAFVWRLRQLLRQAQPDLLHLHSRRGADLWGGLAGRWA
                     GVPVVLSRRVDNPEPRWWVACKYRLYSRVVTISQGIAEVLKREGVPVEQISVVPSAVD
                     TRRFHPGATALPAELGLALPQGAPVAAMVAQFIARKGHELLLEALERLPEHCVRLQVL
                     LFGQGPLREQIAQQISERGLQQRVHLLGFHPALERILPAVDFLIHPALMEGLGVSLLE
                     AGAAGTPAIASRAGGMPEMVIDGETGLLIEPGDVDGLAVAITRLTESDNLRQRLGRRA
                     REWVEERFSLTAMIDGNFKVYQEIMSGLKGG"
     gene            36903..37973
                     /locus_tag="PKI23_00170"
     CDS             36903..37973
                     /locus_tag="PKI23_00170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN35382.1"
                     /translation="MVDSLHLVGSSKLGGAERFVLRLVRALCDAGHDARLIIKRGSQL
                     AEVAAPPALAELSFRNVRDPLAKWETRRAIRRTDAAIVQTYLGRATRLTRLPLGRQPL
                     HIARLGGYYKLDGYRHAHAWIGNTRGICDYLVRNGFPASRVHQIGNFVELPCASPAET
                     LQRLRAQWSGDPAALLLVAVGRLVPVKGLDQLLTAFARLPATLAGRRLELLLVGDGPL
                     REPLQRQAEALGVAERVRWTGWQTQPDPFYELADLVVFPSQERETFGNVIIESWAHGK
                     PLVTSLSRGAQELTRHGETAWQVPCGDAVALADGILELLRDPGLRAGLAERGRAQLLA
                     HHTEAQIIGQYLELYKKLAGGG"
     gene            37974..38732
                     /locus_tag="PKI23_00175"
     CDS             37974..38732
                     /locus_tag="PKI23_00175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNN35383.1"
                     /translation="MAHSITLLMYHQVGAFAPMARHRSTYCDHRRFAAQMAWLKRWRY
                     QVLTLDQAVAALAGQRPLPARAVVLTFDDGYLNFYQYAYPQLRRHGFPATVYLLSDYI
                     GRTADWFAADGRDCPPLLDRARIIEMGDHGIQFGSHGVSHHKLAELDPQEAAQELVQS
                     KSALEALLERPIDHFCYPYGSHSPTVVEQARQAGYRTAVTCVRAAARPGWDWLQLPRK
                     AISYGDTLAGFAFKLAFKNEPKGRLPGFAEVGID"
     gene            38782..39303
                     /locus_tag="PKI23_00180"
     CDS             38782..39303
                     /locus_tag="PKI23_00180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNN35384.1"
                     /translation="MTELDKLGEQLLDPAFIKLAQRQFAKSHPGFQELGIEVVSIGEG
                     RASMRLPYDPKFIGDRNSGSLHGGIITTLMDTLCGLCVMATLRTPTPIATLDLRMDYL
                     RPTRRGLDIIAEAECYSRTKSVAFVRGFALQGDEREPVAHCNASFMLNTKGANFPTSN
                     FSSIHTDAEAGHG"
     gene            39296..39718
                     /locus_tag="PKI23_00185"
     CDS             39296..39718
                     /locus_tag="PKI23_00185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNN35385.1"
                     /translation="MADSFSFLLDQHSADQLIKSIPYADYLGIRIEQDINGQIIYRLP
                     FAEKHIGNPMIRAIHGGVLAGFMECAATLAVIGIIAHDHLPKCVNMTVEYLKSTRPQD
                     TFARVSINNPGRRVVHLTVSCWQEERPVSQAHFRFLAT"
     gene            39812..42334
                     /locus_tag="PKI23_00190"
     CDS             39812..42334
                     /locus_tag="PKI23_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNN35386.1"
                     /translation="MPTPISKKPTPSPQKSVGGRAKRPSGQSEVGEADGASRAELIAA
                     HWSKGARELGAIMLACAALYLAIALLSHNQNDPGWSHTGNSKEVLNVAGLAGAMLADI
                     ALTFLGYLAGLLPILLGIKVVSLVRRPALQPLRPALVAVRAGGLLLTLLAAAPLVTLY
                     VAAPKAGWTGGSGGILGMAIVEAALPIFSVVGSTLLWLMLLAIGITLFSGLSWFWLFD
                     QLGAALIRLSTAFGRALAALPGRLRPAPRAATAADSLKNDPLFGSLDELPEFTLTPRS
                     KAEKRARKPVPPTPTAVKTQSAPLNAEVATVADNVVQISQAAERKARKSAVLADVATA
                     GDEALPSLQLLDPPSAQPLTFYTPESLERTSRQLEEKLRDFGIIVEVVGVEPGPVITR
                     YEIQLAPGIKASRITNLARDLARSLTVMSVRVVEVIPGKAVVGIEIPNEKRKVVRLRE
                     VLDTSIYAALESPVALALGHDTSGVPVVVDLARMPHLLVAGTTGSGKSVGVNAMLLSM
                     LFKATPQELRMIMIDPKMLELSIYNGIPHLLAPVITDMSDAANGLTWCVAEMERRYRL
                     MTQLGVRNLTGFNQKIREAAAAGQPIADPLWQPNPTGRLFDEDPEQPPALQPLPYIVV
                     VIDEFADMIMIVGKKVEQLIARIAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTIIDQSGAEQLLGHGDMLYLPAGTSLPMRVHGAFVADDEVHRVVEEW
                     RRRGTPDYLDEIINGIAGGEGDGGGEGEREESAEQDPLYDQAVAFVTRSRKASISAVQ
                     RQLRIGYNRAARMIEAMEEAGVVSRADVNQNRKVLAPPPAGE"
     gene            42342..42995
                     /gene="lolA"
                     /locus_tag="PKI23_00195"
     CDS             42342..42995
                     /gene="lolA"
                     /locus_tag="PKI23_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNN35387.1"
                     /translation="MSSAGWSGAWLRCLFGLWMLWPLCASAQEVADELSRLLRRNQTL
                     SADFNQEVTDGKGRRLHETHGHFWIKRPQLFRWEVAPPYAQLIVCDGKTLWQYDPELS
                     QVVIKPFDARLAETPALLLSGDTARIVEGFRVTASDVTLGSQSGRRFELTPKAADSLV
                     SQIELFFAGNQLTGMTLRDSLGQNTRLRFFALTVNGVIEADKFSFEPPAGVDIIRDQ"
     gene            43032..44348
                     /locus_tag="PKI23_00200"
     CDS             43032..44348
                     /locus_tag="PKI23_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNN35388.1"
                     /translation="MSEIPAQRDRGYVPLAEQLRPATLDEVIGQSHLLAPGKPLRLLF
                     ESGKPHSMILWGPPGVGKTTLARLIASAFDCQFIALSAVFAGVKEIRQAVEQARESRQ
                     QEGRPTLLFIDEIHRFNKAQQDALLPFIESGLLIFIGATTENPSFEVNAALLSRAQVH
                     PLKPLTSDELRQLLQRAWQRALAPLQFDEAASERLVDSADGDARRLLNLLEQISHAAA
                     VTGHERVDAALVEAALSTGPRRFDKGGDHFYDQISALHKSVRGSHPDAALYWLTRMLD
                     GGADPHYLARRILRMAWEDIGLADPRAIQIARDAAETYARLGSPEGELALAQAVLYLA
                     LAPKSNAGYLAYQQAVRFVGQDQSRPVPLHLRNAPTPLMKQLGHGKAYRYAHDEPDAY
                     AAGESYLPEGMSEPQWYRPVSRGLEIKLAEKLAWLRQRDAARREEQ"
     gene            complement(44380..47070)
                     /locus_tag="PKI23_00205"
     CDS             complement(44380..47070)
                     /locus_tag="PKI23_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN35389.1"
                     /translation="MRPSPRPPRSVTVQALLLTLVLAPILTVCVALALFGLAQRFDEL
                     ETNLQRHAESLLHELAGRYQLLLRQPDPAQLQRLAGDALSDDHLRALHLFNAERQPWI
                     QAGVPLSADRSGLPLTLSNHLEQQQQDDLLRLSLPLGNVEQPSGWLVLEFSRLPTRLA
                     QYQVVFYLLATTVAALLLGLLITLVTTRRLLRPLQQLMVNAERLAAGQPVEPLSRRAP
                     REFLQINQQVEHLGERLAQMRDETQQAIEQSTRDLRQTLETVEIQNIELDLARRESQK
                     ASLAKTRFLADTSHELRTPLNGIIGFANLLLKSPLNEAQRDYLQVIQRSSSDLLAIIN
                     DILDFSKIEANKLVLDRVPFNLRELIEETLLLLATHTFDKPLELVPLIHADVPVHLVG
                     DPLRLKQVLTNLVGNAIKFTPAGSVVIEVRVEQSDAQGALLRVRVIDTGIGIEPEQQQ
                     RIFEAFQQQSPTHGGAGLGLIIAKQLVEQMEGAIGLEQSSAQGSCFWFTLPVTYGLPT
                     SFKAAQLPFNGEPVLLFDTLAASCTAIAAQLEALGLQPQRHEQADSFMAALTQQHWFA
                     VLLGITPPCHPDTIAALCNQVRRHHAGPLLVLSCQALRSGDALPFNDPAMLILGKPAQ
                     SRKLAQSLLQLRGSQPIQPPATAAPRILAVDDNETNLRLLELLLQQLGAEVELASSGA
                     EAIALVSHTRFDLVLLDLRMPGIDGIETARQLRTLPLAKDPRLPIIVVSAHLLDEERH
                     ALSQAGIDDALLKPIDERALRALLSRWIQQPLPIAPANGVVDREEAIRLAGNQPELAE
                     RLLNQLIDSLTAEQQRFSALLSQGDLTALLEAVHRLHGACCYCGVPALRMALRAAEAA
                     LKQSHTAELPRLMAQIDSEIDALLDWQRSHTSLPTMASGG"
     gene            47167..>47737
                     /locus_tag="PKI23_00210"
     CDS             47167..>47737
                     /locus_tag="PKI23_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNN35390.1"
                     /translation="MQPEIPTIENLIGNTPLIRLQRLPGASSNLVLAKLEGQNPAGSV
                     KDRPALGMINRAEARGEIRPGDTLIEATSGNTGIALAMVAAIKGYRMVLIMPDNMSDE
                     RKQSMTAYGAELILVSREAGMEGARDLAEQMAAAGEGVVLNQFANLDNPEAHYCGTGP
                     EIWRQTEGRITHFVSAMGTTGTIMGVSRYL"
BASE COUNT         8612 a        14219 c        15813 g         9093 t
ORIGIN      
        1 cacggtcgat gatgaactcg taggggcggt cgatcacctc ttcgtcacgg ttgcccgaga
       61 cgtaccacat caccaccttg tcgcccttct tgatctgctt gtcacgcaga acggtgtcgc
      121 aggttgcggt gcggcgcatg aaggtcagcg gcgtctgcca gcggatgatc tcgggcacca
      181 tcctgggaat cagacccggg ttgttgcgca gcttctggta ctcctcgggg aactggttca
      241 gtgccagtac gccaccgctg agtgagttgc gagtggtgtc gttgccgccg acgatcagca
      301 gtatcaggtt gccgagaaac tccatcggct gcatgtgctt ggtctcttcg ttgtgcgcca
      361 gcatcgacag caggtcatgc ttgggcggtg aattgacccg ctcattccaa agtccgacga
      421 aggtggccat gcattcgttg accaagatcg actcgcgctc ttcgtcactc tcgatcagcc
      481 cacctggtgt ggggaccgct gtggcgacat cagaccagta ggtgagtttg cggcgctgct
      541 cgaatggaaa gtcgaacagc gtggcaagca tctgggtggt cagttcgatc gacacccggt
      601 cgacccagtt gaaggtctcg ttgaccggca gcgtgtcgag aatttcagcg atgcgttgcc
      661 ggatcagccc ttccatcttc gccaggctgg agggggcgac gatgccctgg acggttttgc
      721 gctgcacgcc atgcttgggt tcatccatcg cgatgaacat tttgagcggg agctcttcgc
      781 gtggatcggc aatgccgata ctgggttcag aagagtagat ctgcggattg ccttcgaccg
      841 tcatgatgtc gttgaacttg gtcaccgacc agaaggggcc gttttcggaa tccttgcagt
      901 agtgcaccgg ttcttccttg cgcagccggt cgaaaaatcc ccacatggtg ttggattcga
      961 acagcgccgg ttgggagacg tcgtaatctt cgagcggaat gctgtaggga tccttcccat
     1021 acatgtggtt gtaggctttc ttgacagcag tcatggtgaa tcacctgcgg atgtgaatgg
     1081 atctgaagga ccgtatgcac acgaagatgc ggcagacgca ccatcgctcg atgaatcatg
     1141 acacgcgacc gaatgaccgt ccagtcgatc cgggcttgat tttgccgcaa tgctggctac
     1201 agcgattcaa gcggatcgac agcgatgttc cagtgcaggc cggcgggcgt cggcagccgc
     1261 tccagttcac tggccaatcg gcgcagcagt tgctgcaggt gggggcggtt gcgggcgatc
     1321 agcagaatct gggcatggtg gcgtccgcca cgccggtcga tcggcgcggg attggggccg
     1381 agccattcga tgccgctgtg tggtgggggt ggggtcaatc cctgcagaaa gcgttgggca
     1441 gcctcgggtg cgggcgcatc agccagaatc agtgcgaggt ggcgataggg tggcagtgtg
     1501 gccatggcgc gtcgctgtag ttcggccgcg gcaaagtggc catagtccgc ttcgatcagt
     1561 tcgagcagtt gcggatggtc ggggtggtgg gtctgtatcg ccacctctcc cggccgctca
     1621 gcgcgcccgg cgcgcccggc gacctgggtg accagttgcg ccatctgctc catcgcccgg
     1681 aagttgctgc tgaaaaaggc gccgtcggcg tcgaggatgg cgcagagggt gacgcgggga
     1741 aagtggtgtc ccttggccag catctgggtg ccgaccagaa tggcgggcgc ctgccgttcc
     1801 acctgttcca gcagttcggt caaggtcgcg gagttgtgcg ccgtgtcgcg gtcgatgcgg
     1861 atcagcggca catcgggaaa gcgctgtgcc agcgtctcct cgcagcgctc ggtgccgctg
     1921 cccagtgcga tcagcgtctg gctctggcac tgcggacagg ccttgggtgg agtgcggcgc
     1981 tgatcgcagc gtgggcagcg cagtcccggc cgatgacgat ggagcaccag gcgtacttcg
     2041 cacagtgggc aggtggccat ccagccgcaa tcgtggcaga gcagggcggt ggcatagcca
     2101 cgacggttat ggaacagcag cacctgattc ccggccgtga gatgttgatc gatcagcgcc
     2161 agcagcggca gcgaaaaacc ctgttgcagc ggacgaccgc gcagatcgat cagacggatc
     2221 ggtggcaatg tcgcattgcc ggcccgctcc ggcaatgaaa ggtggtggta gcggccgcgc
     2281 tcgacgttgg ccagactctc cagcgacggt gtggcgctgc cgagcagcac cggaacctgc
     2341 tcgatctggc cgcgtcgtac agcaaggtca cgggcgcagt agtggacccg ttcctcttga
     2401 tggtaggagc tgtcgtgctc ctcatcgacg atcagcagcg caggccttgc catcggcaca
     2461 aagattgccg agcgggtgcc caggatgatg tcggccgtgc cctgtgcact gtgcagccag
     2521 tcgatcatgc gcaggccggc agtgcggccg gagtgcagca cggcgatacg tgcctgcgga
     2581 aagcgctgcg cgaaacgttg ctgcaattgg ggtgtcaggc cgatctccgg caccagcacc
     2641 agtgcctgtt gcccgcgggc cagcacatcg tcaatcaatt gcagatagat ttcggtcttg
     2701 ccgctgccag tcaccccttc gatcagcgtg acgctgaacg ctccccagcg ctgccgcatc
     2761 gccgtgcagg ccgcttgctg atgtggattc aacggtggtc cggtttctgc tgtgctggat
     2821 gcagacgggg cgtgcgagct gggcagccgc cagtcacagc gcagcagccc ttttttcagc
     2881 agcgcagtga gcggggcgcc agtcagaccc agcgcgcgca actcggcatg gtccagaccg
     2941 ctgtcgcggg tcagacgggc gaggatctcc tgctgcttgc ggctgcgccc agtggctttc
     3001 gtttcatgca gggtggctcc ggcaggcgaa agccggtagt gccgctccat caccttgcgc
     3061 agtggttggc cgtgccgcaa tggcggtggc agagtggcgg ccagcagttc accgatcggc
     3121 tgctggtaat actcggcact ccagagggcg agttgcagca gtggctgtgg tagcagggga
     3181 tgttcgtcca gcaccgtttc gacagggcgg attttgccgg cagcgactga actccgtgct
     3241 gcaatggcga ccaccacccc caccagcagg cggccagaga agcgcacccg tactctggtg
     3301 cccacggcca tcggttcggc cgatggtgcc aggtagtcga aggtgcgccg cagcggcacg
     3361 ggcagcgcga cctgcaagta gagcgtctct tcgctgtgca tcacgagagt ggatcagcga
     3421 ttgcgacgga gtggtctggc gcaacagaga tcaggcggga tgggttgcac tgcggcgctg
     3481 atcaggtgcc atgcgtggct gggacggctg ccaagtgcgg cagtgggcgt gacctttgtt
     3541 ctcggttctg ctagcatcag cgccctttat gtgactgggt tgcctcagcg gcgggttgcg
     3601 actgtgctgg gggattctag ctgggttcga tccgaatctg taacgaatgc attccaaatt
     3661 gtgccgcggg aggagcaatg aaatctgaca ttcaccctaa atacaccaaa atggtcgctt
     3721 cctgccgctg tggggcaacg atcgagaccc gttcgacgtt gggacgtgac atgaacatag
     3781 aggtctgctc caactgccat ccgttctata ccggcaagca gaaggttctt gacactggtg
     3841 gccgggtcga gcgtttccgc aagcggtttg gccgtcccag cgtggcagct tcgaccgagt
     3901 aaacctcagc ggtacagcgc cgctgtcgtc atcaggccca tgccagcccg aacagggctg
     3961 gcatgggcct tttttgtctt cctctgaaat cgactgaggc tgccggatgg cagcttatca
     4021 gacatgcctg atgtgaacag agctgtttgt cggtcgccaa tagcaaattt tccagaaaat
     4081 tgcttctaat ctggtctgtt tgaaaccgat gtcttgcgct attctggcag aaacacctgt
     4141 tcaaccgacc agtcttggag gatttggcat gtctgacagc ctgaaacaga ccgctctgga
     4201 atgccatgag ctgcccaggc ccggaaaact ggagatctct gtcaccaaac cgaccggtac
     4261 agcgcgtgat ctggcgctgg cctacagccc cggcgtggcc gaaccggtgc gagagattgc
     4321 caatgatccc gaaaatgcct accgctacac cgccaagggc aatctggtcg gtgtgatctc
     4381 caacggttcc gccatcctgg gcttgggcaa tctgggacca ctggccagca agccggtgat
     4441 ggagggcaag ggggtgctgt tcaagcggtt tgccggcatc gatgtgttcg acatcgaagt
     4501 cgatgccgaa acgccgcaag ccttcatcga cacggtggtg cgaattgccg agaccttcgg
     4561 cggcatcaac ctggaagaca tcaaggcccc ggagtgcttc gagatcgagc gggttttgat
     4621 cgagcagtgt gcgattccgg tgtttcatga tgaccagcat ggcacggcga tcgtgaccgc
     4681 ggccggactg ctcaatgcgc tggagttgca agggaagcgg ctcgaagagg cgaccatcgt
     4741 ctgccttggc gccggtgcgg cggcgattgc cggcatgcgg ttgctgatca gtctgggtgc
     4801 cgacccggag cgcatcttca tgctcgaccg caagggcgtg atccatgcag accgtgacga
     4861 tctcaatcaa tacaaggcgg cttttgcgcg ggaaaccgac aagcgcagtc tggccgatgc
     4921 ctgtgcgggt gccgacgtct tcatcgggct gtcgggaccg aacctgtttc cggcgcaggc
     4981 gctgaagatg atggcgccgc gtccggtggt gtttgcctgc tccaatcccg atccggagat
     5041 cagctggcag atggcccatg cagcacgaga tgacctgatc atggcaaccg gccgatccga
     5101 ttatcccaat caggtgaaca atgtgctggg ctttccgttc atctttcgcg gtgcgctcga
     5161 tgtgcgcgcg cgggtcatca acgagccgat gaagatcgcg gcggtacatg ccttgcggga
     5221 gctggccaag gcgccggtgc cgcaggaggt gatggatgcc tatgaggggc ggcggttgag
     5281 ctttggcccc gaatacatca ttccgaagcc gatggatcca cggcttctcg gacaggtctc
     5341 agccgcggtg gcgcgtgccg cggtcgactc gggtgtcgcg caaaaatcct atccagcgca
     5401 ctacccgctc acatcgaccg atgaggtttt tggcggtggt cgcaccggct gatgggtgag
     5461 gcggcatcga tctggcgtac tgcattgttc agaacagttc gcggctgtcg gtggggctcg
     5521 gagccgcgcc ttccggcgag cttgacggtg gggagcgtgt ctcctgctgc ggcagctctt
     5581 cggccttgaa cagttcgaag atggcgtggc tcatgtccgg accggcacgc tctccagtca
     5641 ccgggtcgat gcgcactgcg acgatcccgg gtggttgcgg cagctgcact tcgtcgacgc
     5701 catcgagcgc agtgcgcatg aaatcgatcc agatcggcag ggcggcagtg ccgccgaatt
     5761 catattcgcc gaggctgcgg ggctggtcgt agcccaccca caccgtggtc accagattgg
     5821 ggttgaagcc ggcgaaccaa gcatccttgc cttcgttggt ggtgccggtc ttgccggcga
     5881 tgtcgtggcg gttgagagcc aaggcagccc gaccggtgcc cttggtgatc acatccttga
     5941 gcatgtcgtt gatcaggtat tgggtacgcg catccatgac ccgtggcgcg ctcttcgagg
     6001 cggcgctccc atcgattgcg gccgggtgtt ccggagcggg attttcggtg tcgcactcat
     6061 ggcagacccg ggcagggttg gcgcggtaca gctcctgtcc gtagcggtcg tagaccgcct
     6121 cgatcaggta gggttccacc ttgaagccgc cattggcgag cacagtgtag ccgcgggtca
     6181 tctcgagcgg cgtgaccgtg ccggtgccca gcgccagtgt cagatcgcgt ggcagccgct
     6241 ccttggcaaa gccgaactgc tgtgcgtagc tgattgcctt gtcgggccca atcgcttgca
     6301 gcaggcggat cgagaccaga ttcttggaga ggtagagcgc ctggcgcagg cgaatcgggc
     6361 cgctgaaatc cccgcccgca ttttcggggc gccactccgg ctcgaaggga gaggtctgaa
     6421 acaccagtgg ggcatcgtca atgatgctgg ccgcggtaaa gccattttcg agcgcggcgc
     6481 tgtagatgaa gggcttgaag ctggagcctg gctgtcgctc gccctgggtg acgcggttga
     6541 aatggttgct ctcggcgttg aggccgccca ccagtgcctg gatggcgcca ctgttcgcat
     6601 cgatgcagag cagtgcggtc tggacctggg gcggctgggc cagaatccag ctgccatcct
     6661 gctccctgaa cagccggatc agatcaccgg gagaaaaagg atgtttcccg ggtttggggt
     6721 tgcgtggcag ggcgacctga ctgatcttgc gcccgctcca gcccaggagc ttgtcccagg
     6781 gcaggctcaa ctcctcgcca ttttcgagca ggatatcgat ctgcttgcca tcgatgcggg
     6841 tgatcagtgc gcgctgcagg ccgctgaagg cgggcagcgc catgatcctg gccagtcgcg
     6901 cgtcgcggct ggtcacctcc ttcaggtcga tctgtccttc gctgccgcga tagccatggc
     6961 gccggtcata tttgagcaca ccggagagtg tggcctggtt ggcgctctgc tgcagtcggg
     7021 catcgagggt ggtgatgacc tgcaggccac tgctgtaggc cttgctgcca taacgggcca
     7081 gaatctcctg gcgtgccatc tcggccacat gctcggctcg caggtcacgt tgcgggccgt
     7141 gatagcgcgt ggcgggcgga acggtgatgg ctgcgtcatg ctcggcctga tcgatgtacc
     7201 ccagtgagag catgcggccg agaatccagt tgcgacgcaa tccggcccgc tcggggtcgg
     7261 cgatcgggtt gaaccgagac ggggccttcg gcaatgcggc gatcatggcg atttcgctca
     7321 ggtcgagggc gtcgatcttc ttgccatagt agacttcggc ggcggcctga atgccgtagg
     7381 cgcggttgcc gagatagatc ttgttgacgt agagcgcgag aatctcctgc ttggagagtt
     7441 cgcgttcgat cttgatcgcc agcaggatct ccttcagctt gcggtcgaag gtccgctcct
     7501 gagacagaaa atagttcttc gccacctgca tggtgatggt gctgccaccg cttttgatgc
     7561 ggccgctttg gatcagctcc agcgcggcac gcagcaggct gccgggattg atgccgggat
     7621 gtgaaaagaa ctggtcatcc tcggcggcca gaatggcctg gatgaaccgt tgcggaatct
     7681 cctcgtaggt cagtggcgag cgccgttgct cgccaaattc tccgatcagc agcccctctt
     7741 tcgagtagat cagcagcggg gtttccagct ccacctctcg cagcagctcc acgtcaggca
     7801 gtcgcggtac ggtgtagagg tagagtccga acagtgccag cgtgaagagg cagagcgtgg
     7861 caagcgtgat tcgtatcaat tgcagcatga agagcgggcg tggtgtgaag agtggtggag
     7921 tgactggcaa actgatcggc aaggactact attgaaaaag attggatttt cggtgcctgt
     7981 cacagttctg cattctaacg catgggccaa ggggtgcggg atgtgaaatg gtccgcatcg
     8041 ctccggaagc aggagatgtc tgctgaaaat cctgtttgcc tgttgggttg caggtggtat
     8101 ttaatgtgaa gggtcgagat atatctccgg cgtgcgcaag caaggaatca ttctgacgtg
     8161 tttggtctgt tcaaaaagaa gcaaacgccc ctgctcggga tcgacatcag caccacggcg
     8221 gtcaaactga tcgaactgag ctgctccagt gacagctatc gggtcgagag ctatgcggtc
     8281 gagccattgc cgcccagcgc ggtggtcgag aagagcatcg ccgatgtcga ggcggtgggg
     8341 caggccatcg gcagggtggt gtcccgtgcc aagaccaagg cgcgtgatgg cgtggtggca
     8401 gtggctggat cggcggtcat caccaagacg atcgaaatgg atgccgatct gaccgacgac
     8461 gagcgcgaga cccagatatc gctcgaagca gaccagtaca ttccctatcc gctcgaagag
     8521 gtggccatcg atttcgaagt gcaggggccc agtgagagcg acgccagaaa ggtgcaggtg
     8581 ctgctggccg cctgtcgtca tgaaacagtg gaggcgcgtg aagccgttct ggccattgcc
     8641 ggactgacgc cccgcatcgt cgatgtcgaa gcctatgcga tagagcgtgc ctttttgctg
     8701 gtcaaggatc agatcgaccc tgatgcccgg gtcgtggcca tcgtcgacat tggcgccacc
     8761 atgaccacgc tcagcgtgct ggtcgaaggc aagaccatct acacccgcga acagctcttt
     8821 ggcggcaaac agctgactga cgaaatccgt cggcgctatg gactttccga agaggaagca
     8881 ggactgggca agaagcaggg cggattgcct gatgaatatg aagatgaagt gctggcaccc
     8941 tttcgtgagg cggccgtgca gcaggtggcg cgctcattgc aattcttcta tgcatcgagc
     9001 cagcacaatg acatcgattg cctgattctg gcaggtggaa cctcttcgat ctccggcatg
     9061 acagagcttc tgcaacagcg aatcggtgtt cccacttttc tggccaaccc ttttctcggg
     9121 atgaccattg cccccaaggt caatgccgcc gcgctgacca atgacgcgcc cgccttgatg
     9181 attgcctgcg gtctggcgtt gaggagcttc gactgatggc caagatcaac ctgttgccat
     9241 ggcgggaagc gctgcgtgcc gagcgtaaaa aacgctatat gatcgcgctg accgtgaccg
     9301 cgctggtcgc ggtgctgctg gtgctggggg gcagtcgcta catccaggga gagatcgaga
     9361 atcagcaggc gcgcaatcag ttcatgcagc gtgagatcgc gcagctcgat gcgcagatca
     9421 aggagattct ggggttgaga aaacgccggg acgagctgat cgaccggatg aaagtgatcc
     9481 agtcgctgca gggcaaccgg ccggtgatcg tgcggctggt cgatgaattg gtcagaacat
     9541 tgcctgatgg cgtctactac acttcgctcg ataaacaggg tgagcagatc accattcagg
     9601 gtgttgccaa gtcgaacaat cgggtctcgg cgttgatgcg caacttcgac cgttccgagt
     9661 ggtttgccaa tccgaatctg cttggcgtca aggcatttcc cgaattcggt gaacaggcca
     9721 acagcttcaa cctgcttgtg cagcagaaga atccagacgc tggcgaagcg aagaagcaat
     9781 agggtgtttc gtggccattc ttgatcgaat caaaaacctt ggaaagatcg atttcagcaa
     9841 gatcgatctg aacgacctcg atctcgacaa catcggctcc tggccgatgc cgatcaaggt
     9901 ccttctggcg ctgctgctgt ttgcagcact gcttggatca ggatactggt tctatctcaa
     9961 ggagatggag cttgagctgg agactgcggc gcgccaggag cagcagttga aggagagctt
    10021 ctcggccaag tcgttccagg ccgccaacct cgagcagtat cgccagcaga tgaaggagat
    10081 ggaggagtca ttcgggaccc tgcttcagca gttgcccaag gacaccgagg tgcctggact
    10141 gctcgaggac atcacccaca ccgggctcgg ttccggactg gagttccaga gcattcgcct
    10201 gctgcccgag gtcaagcgag aaatctacat cgaactgccg atcgagatcg ttgtcaccgg
    10261 cagctatcac gatctgggca gcttcgtcag tggtgtggcc gcattgccgc gcatcgtgac
    10321 tctgcatgat ttcagcctca gacccgacag caattccggt ctgttgacgg cgcgtatcat
    10381 cgccaagacc tatcgttaca aggatggggc agcacaatga ttcgggcatc gacgagatgg
    10441 ggattggtat tgctgtgcct cctgttgcag ggctgtggtc aggaagatcc gtttgccgat
    10501 ctgaaggcct atgtggaaga ggtcagggcc aagcccaagg ggacgatcga accactgccg
    10561 aaattcaagc cttatgaatc ctttgcctac tcggcggcgg gaatgcgcag cccctttgat
    10621 cgaccgatcg aaatccaggc cgtccagcgc cggatggagg gagagaaggt ggcgccagac
    10681 accaaccggg ccaaggaatt ccttgaaggc ttcaacatcg atgccttggt gatggtgggc
    10741 accattgcca aaaccgacca gatctgggcg ctgttgagtg atggcagtgg cggcatacac
    10801 cgggtgaccc agggcaacca tctggggcgc aatcatggtc gtgtggtggc ggttcacgag
    10861 aacaagattg atgtggtcga gatcgttccg gatggacagg gtggctgggt ggagcgtcca
    10921 cgcaccctgg acatgaagga atagtcggcc ccgattggat attgatggag agaagattca
    10981 tgacgttgat gcgggcaaca aaaaaagcat tgcaagatgg ccgctggatc gcgtcgcggt
    11041 tcgttgcgct gtgggggctg ctgtttgcat tgggcgtcca gggggcggca ctgcaggggg
    11101 tcgagtttgc atccctgccg aatgaccgtt tcgagatcag gctcgatttc gacggcgctc
    11161 cgccccagcc tcaaagctac agcatcgaac agccggcacg catcgccatc gacctggtcg
    11221 gggtgaagag tgcgctgggc aaaaagagct attcgctcga ccaccgcaat gcccgcagcg
    11281 tcaccgtggt ggagagcaag gaccgcaccc gcatgatcat caatctggtc gatcttgcgc
    11341 cctacagcac gcaggtcgat ggcaatacat tgcgggtggt cctgggtgaa aagcaggctg
    11401 ctgccgtgac ggccgctacg cctgcgccgg tggctgcccc agtggcagca gcgccgactg
    11461 cggtacccgt ggcggctgca cccccgcctg ccgcagtcgc ggtgactcga cctgcggtgt
    11521 tgccagcacg tggcagccag gtcgaaaagg tcgattttcg gcgtggcgag agcggtgagg
    11581 gcaagctggt ggtgacgctg tcgaacagcg gtgccaacgt cgatgttcgc gaagagggtg
    11641 gcaagatccg cgtcgagttc agcgacacca gactgctgcc agccttgcgg cggcggttcg
    11701 atgtcaccga ctttgccacg ccgatcacca gcatcgatgc caccgaggag tcgggcaagg
    11761 cggtgatgac ggtgacgccg accggccact atgactacct cgcctaccag gccgacaccg
    11821 agttcactct cagcgtcaaa ccgctgactg ccgaggagca ggccgacgaa cagaagaaaa
    11881 agttcgccta caccggcgaa aagctgtcgc tgaacttcca ggacatcgaa gtgcgggcgg
    11941 tattgcagtt gatcgccgat ttcaccaacc tgaatctggt ggcgagcgat acggtgaccg
    12001 gtcgcatcac gctgcgactg aagaatgtcc cctgggatca ggcgctcgat ctggtgctca
    12061 agaccaaggg gctcgacaag cggctggtcg gcaacgtgct gacggttgcg ccggcggccg
    12121 agatcgccgc gcgtgagaag cttgaactgg agaccaacaa gcaggtcaat gagctggcgc
    12181 cgctgcacac cgacttcatc gaggtgcgct acgccaaggc ggccgacctg ctggcgatgc
    12241 tgaccggcaa gaacagcatc ctgtcgacgc gcggcaccgc cagcatcgac aagcgcacca
    12301 acctgctgat cgtgcaggac accgatcagg tgctggccga ggtgcgcagc ctgctgaagc
    12361 gcctcgatgt gccggtgcgg caggtgctga tcgaagcgcg catcgtcaag gccagggcca
    12421 atgtcagcaa ggatctcggc atccagtggg gggggcgcta tggcgataat gacggcaaca
    12481 acatggtgat tggcgggtct ggcgtgaggg ccggtgtccc tggaccggtg accggtggcg
    12541 atgtcgtcaa cctcggcgtc accaaaaccg gaaccaccac gctcaacctc ggctttgtca
    12601 acaactccaa catcctcaac ctgagcctga ccgcgattga agagaatggc gacagccaga
    12661 ccctggccca gccgaaggtg ctgaccgccg acaagcagat ggccaagatc gagcagggca
    12721 ccgagatccc ctatcaggag gccacctcca gtggcgccac ctccacctcg ttcaagccgg
    12781 ccgtgctggc gctggaggtg acgccgcaga tcacgccgga caaccgcatc atcatggacc
    12841 tgcaggtcaa tcaggatgtt ccgggagccg tgaccaatgg cattccgagc atcgacacca
    12901 cccgcatcaa gacccaggta ctggtcgaca atggccagac ggtggtgctg ggcggcatct
    12961 acacgcagga cgacactttc acggaaacca agactccgct gttgggggat gtgccctatg
    13021 tcggcagcct gttcaaacgg acctccaagg gcaagatcaa atcggagctg ctgatcttca
    13081 tcaccccgcg gctggatggc gagggcaaaa gcagcctcca gtgagcaggg cggatcgcaa
    13141 catatttctg atcgggccga tgggggcggg caagagcacc atcggccggc tgctggcgga
    13201 acgtctcggt ctgccgttca aggattcgga ccatgaaatc gaagcgcgca gtggtgctga
    13261 cattccctgg attttcgatg tcgaaggcga agcgggcttt cgtcgacgtg aaactgccgt
    13321 actggccgac ctgacctgcg agcagggcat cgtgctggcg accggcggtg gcgcgatcct
    13381 gaaggaagag aatcgcacgc tgccgaaaca gggtggcacg gtggtctacc tggctgcctc
    13441 ggtcgagcag caatatgcac gcacccgccg tgaccgccag cggccgctgc tgcgcgaggc
    13501 cgatccccat gccaggctgg cccagctctt tgccgagcgt gagccgctct atctgtcgat
    13561 cgccgactgt gtggtgacca ccgaccggcg cacgccgcgg ctggtggtac aggagattct
    13621 gcgacatctg ggtcgggacg atcccacgac agcctcctga tttcgttcct gagtgcggtc
    13681 ggtgaagtca cgcccaccct gcatcgggtg agcccgaatc ggtggtcccg ggtgcaaagg
    13741 gctcaccatc aatcaaatca atgggttatc attcggtctg gtgctgaagg atggccgatg
    13801 ccggactttc agcggttttg gtgaatcgtc gtcgaacatg tcatctggac atgaagactg
    13861 cgcactcaag tcccggatct gattcatgct gcaactcact gtcgaccttg gcgcgcgttc
    13921 ctatcccatc cacatcggcg cggccctgct ggcgcggcct gagctctttg cgacggcgat
    13981 tcgcggcgaa aaggtgctga tcatcaccaa cgatcgcatt gcgccgctct atctccagtc
    14041 ccttttggcc acgctggcaa accatcgggc cgaggtgtgc atcctgccgg atggtgagca
    14101 gtacaaaacc gtcgagagtg cgatgcagat ctgggatcga ctgattgcgg ggcaccatga
    14161 tcggggcacc accctgctgg cgctcggcgg aggagtgatc ggcgatctga ccggattcgc
    14221 tgccgcctgc tatcagcgcg gtgtcgattt tgtgcaggtg ccgaccacac tgctggcgca
    14281 ggtggactcc tcggtgggtg gcaagaccgg cgtcaaccac ccgcaaggca agaacatgat
    14341 tggtgccttc catcagcccc gcgccgtcat catcgacacc aataccctga acacgctcgc
    14401 accgcgcgaa ttctcggcag ggctggccga agtgatcaag tatggactgc tcggcgatgc
    14461 cgatttcttc ggctggctcg aagccaatat ggagcagttg atggcgcgcg atccggatac
    14521 cgttgccagg gcaatcgagc actcctgtcg ggccaaggcg ctgatcgtga tggccgatga
    14581 gaccgaacag gggcagcggg cgctgctcaa tctgggacat acctttggcc acgccattga
    14641 atcagtgaca aattatcggc gctggctgca tggtgaggcg gtggcgatgg ggctggtgat
    14701 ggcggccgat ctctcgcggc ggctgggcat gttggacgag gtcgaggtcg ggcggatcaa
    14761 gcggctgctg cagcgtgctg gcctgccatc tcatccgaca ccggacatgc agagcgaggc
    14821 gctgctggca gccatgcaga tcgacaagaa agtacagggt ggaaaactgc gggtggtggt
    14881 gatgcgtggc attggtgcgg ctgaattgct gaccagcccg gcagtcggac tgctgcgcga
    14941 gacaatttcc gctgccatcg ctggaagcat ataatggctt tccattatcg atggttggca
    15001 tgaaatcttc agaatccacc tcaagaggcg gcgagaatga cacaacatga tgcaggtcac
    15061 gaaagagcgg gttcggcctt ggctgggctg cgtgccactg atgacgctgc gctggagcag
    15121 gcgcttgccg caccgtcgat accgttcttt ccgggcgctg gtcgcgaaca actgctgacc
    15181 caactggagc actggagccg ttatggcaat ggcatcctga tcgtcgaggg cagcgagcag
    15241 agtggaaaaa ccacgctggc tggagagttg catcagcggt tcggcaatga gatgaccctg
    15301 ttgtcgatct gtgccagcgg gcagacgcaa tcaatcgacc tgctgatcga aattgccgat
    15361 gcgctgggta tcgagtcggg actggaaccg gatgaggctg acctgctgga gaagattcag
    15421 caaatcgcgc aagaagccca agcggatgcc agtgccgtgg cgatcgtgat cgatgatgcc
    15481 catgaactcg atgaggccag ctaccatctg ctgttcaggc tggcgttcaa ccctgaagac
    15541 aaccagccgc tgatttcggt gatcctgctg tcgctgccgg gcatcgatct gtggcgcaac
    15601 gtgcccgaag gcatggcctg gccgacactg gcagtgctgc gacccctcga tttcgaagag
    15661 gcgctcgagt tctggcggca ctgtcggcaa cagagtggtg gatcggctga cgccatcgag
    15721 gattcacgga cggcacggag gctgtgggct gacgccgaag ggttgccggg caacctgctg
    15781 cggcgttttc ccgaccagaa ggaaagtgct gccgcggccg tgccaagccg tgcggcgctc
    15841 ttttcattgc cgaactggca tctgctggcc atcgtggcag tgggtttgct gctggcggcg
    15901 gtactctggt ttggcaaccg tgagtccgcg cctcctgagc agcaatcgct cagcatcgcc
    15961 ctgcccacgg agcctgccgc gccggtcgtg ccgctccctg cggcgccacc gtcatcagag
    16021 gctcagcccg cgccaacaac gctgccgcct tcaatgccgc cagtgtcaac gccaattgcc
    16081 agcgaacagc ctgccgtggt accggcgccg gtacccgctc cactgcccaa gccgaaggtg
    16141 attccagcgc cagttgagcc gcccaaggcg gttgaaaaac ccaaacctgc gccagtcaag
    16201 gagcctgcca aggcgagcaa accggccact ccgccgaagc aggcggtggc cgctgtgccc
    16261 gcatccaagg cgacgctggc gacaccggaa gtggcgcagc agcatcaaaa actgctcgca
    16321 ctggcgccag aacggttcac cctgcaactg ctcggcgctt cggatctgag caaggtgcgc
    16381 aactacatcg atcgcgaact cggttgcaag gattgctact actatgaaac ccggcgcgat
    16441 ggcaagccct ggttcactgt agtgaagggg gtctacgccg atcgggcggc cgccgtggcg
    16501 gcgcgtgaca agttgccggc caaggtcagg gcgcagcagc catggccgcg cagtgtgcgc
    16561 gacatccaga aggagctgcg tcagcagcga tgacgccgaa agagggtgtg catgaagcgc
    16621 cgatcggtca ggcgctctca ttgccttttc ccgtgaccgg cgatggttgt tttccgggct
    16681 cccgaactgc cttcaccagt caatgatgtg caggattggc gttgtcgtgc cccgagccct
    16741 gatatatcct gccgaagcac gttttgcgtg ctttcgtcaa atcaaccgct tggagaatcg
    16801 atatgggcag tcgagtgata aaactgcgtt tcttgcaatt ccttctggct ttcatgttgg
    16861 cgttgggagg tggtctggca tcgtcgttgg cgtggtcgga agtggcgaag atcgacatca
    16921 acagtgccga tgaggccacc ctgtcaacgc tcgatggcgt cgggcctgcc aaggccaagg
    16981 cgatcatcga gtaccgcaac aagaacggca agttcaagtc gatcgaggag ttcgagaagg
    17041 tgccgggcat tgggcctgcc acgctgaagc agaacgccgc ccggttgacc gtgaagtgac
    17101 cagcggcagt gatgcagttt gataccgagc agttctcacg gggtgtggag ccggcattcc
    17161 agcgacgccc ccccgtgtct gcgcagagtc ccgtcaacgt acagcacgga tggccaggca
    17221 gaccgttgga ccacgtcact catctccttg agccaatccg gatgcatccc caatgaacga
    17281 ttcgtcgacg tcggcggagc gaagcgctgg attctggccg attggcaacc gttttcgtgg
    17341 ctttctgccg gtggtggtgg acgtcgagac tggtggattc aatgcccgca ccgatgcgct
    17401 gctcgaaatt gccgcgaccc tgctcagaat ggatgaagag ggctatcttc attgcgagca
    17461 gacgcatttc tatcgcgttg ccccctttgc cggcagcaac atggaacctg cggctctggc
    17521 cttcaacaag atcgatccct accacccgtt tcgtttggca aaatctgaag acgaggtgct
    17581 gaccgacctg tttcagcatg tgcgaaaagc gatcaaggcc aaccactgca ccaaggccat
    17641 cctggttgga cacaatgcca gctttgatca tggatttctc aatgcggcgg tggcgcgttg
    17701 tcggatcaag cgcaacccgt ttcatccatt ttcgagtttc gatacggcga ccctggcggg
    17761 tctggccttt ggccagaccg tactggcacg cgcctgccgc tgcgccaacc tcgaatttga
    17821 caatgaccag gctcactcag cgcgttacga caccttcaag actgccgaac tcttctgttt
    17881 catcgtcaac gagtggaagc gtcttggcgc aaagccaccg aacagtgagg gcgaagtcga
    17941 cggtgaagcg gtggatgacg aggtcgacga ctgaagctga ggttgtcgca agattccttg
    18001 cagtggctac tattatcaaa gagcgtttcg acgccagcat tggaattttt cgttagatcg
    18061 gctcaagggc ttgataatga taaaaaatta tctgttatcg cttggtttgt acgcgctgct
    18121 tctgccgtgg acggctgttg tgcaggcgct ggatgccgcc gaacagcggg gtcttgagat
    18181 cgccagggag gcggatcggc gcgaccaggg ctggggcgac agctcaacct cgatggagat
    18241 gatcctgcgc agcaaggagg gtgaaacggc gagccgtgcc ctcaccgtca aggtcaagga
    18301 ggggcagccc gctgtcgaag agggtgaccg gtcactgatc gttttcaact cgcccaagga
    18361 tcagaagggc accgcgctgc tgacctacag ctaccgcacc aggtccgacg agcagtggct
    18421 ctaccttccg gcgctcaagc gggtcaagaa gatcgcctcc gacaacaaat cgggaccctt
    18481 cgtcggcagt gaattcgcct tcgaggattt ttccgaccag gaggtcgaga aataccgcta
    18541 ccgttatctg cgcgatgaac ccttcaatgg catcgactgt tttgtcatgg aacgctatcc
    18601 gctcgacagc aactccggtt acagccgcga actggtgtgg atcgatcagg cgcattaccg
    18661 gatccacaag gtcgactact acgaccgcaa gcagacattg ctgaaaacgc tcagcgtcag
    18721 cggctatcag caatataacg gtaagtactg gcgtgccgat ctcagcgaaa tggtcaatca
    18781 tcaaaatggt cgaagcacct cgctgaaggc ggccgattac cgcttctcgc aggggttgag
    18841 cgacgatgag ttttcacaga acagcttgca gcgggcgcgt tgatgatgaa aaagttgggc
    18901 agcgcgcttc tggccacact gctgtggaca gggtcggcat cggcgtatga gctcgatggc
    18961 gagctggcgc tcgagggccg actcttcgcc gaccgcggtc cccgtggaca ggagcaactg
    19021 gatggcgcga tctggacgcg ctggcaatat ttcaacgact ggaatgacgg caaggacagc
    19081 atcacggtcg tgcccttcct gttgcaggat cagcgtgatg ccgagcgcag ccattgggat
    19141 ttccgtgagt tgacctggct gcatgtcggt gatggctggg agttccgcgg cgggttgcgc
    19201 cgggttttct ggggtgtcat cgagtcacag catctggtcg acatcatcaa tcagaccgat
    19261 gcggtgctgg cgatcgatgg cgaggagaaa cttggacagc cgatgctcaa catctcgacc
    19321 gaaggccgct ttggcaccct cgatctctac ctgatgagct tctttcgcga tcggaccttc
    19381 ccggggatcg acggccgtct gggcggaccc ttggtggttg ccgatgacag cagccggttc
    19441 gaggccagtg atggtcgccg tcatgtcgat gtggccatcc gctgggcgca caatctggag
    19501 aactggaact tcgcgctctc ccacttttcg ggcaccagcc gggaaccact ctttcagctt
    19561 gaatacctgg gcgcctcacc catcccgacc gggcgactga ttccactcta tccgaccatc
    19621 gatcagaccg gccttgaact gcaatacatc cagggaggct gggcctggaa gctggaggcc
    19681 atttcgcgca gcggttatgg tggtgatcga tatgccgctt caggattcgg tttcgaatac
    19741 acccagaccg gtattgccgg gagccgcatc gatctgggct ggatcgtcga gtacgactgg
    19801 gacgagcggg gtgaccaggc accacccggc gtgctcgagc atgatctgtt cctgggtacc
    19861 cgctgggcct tcaacaacct gggtgaatcg cagctgctgg ccggcgtcgt ggtggattcg
    19921 gacagcaggg agcaggtcta ctggttcgag ggcagccatc ggttgagcga tcagtggaag
    19981 ctggccttcg agagccgcat ctttgccggt ggtcatctgc cgcccaccga tctgacttcg
    20041 attctgaccc aggctgcaca gcccgatttt gacaacaagc tcggttactg gcagaaggat
    20101 gatttcgtcc agttgcaatt gatccgctac ttctgacaat cgatgtcgct gtcagccgcg
    20161 ggatgagtcc gatttcacca tgtttcttcg tgtctcgctg tcacggcagc tgctcgatct
    20221 gctcgatgag catggcggtg tgcagcgcag cttcgccatc tcatcgagtg cgcgtggcgc
    20281 gggtgaactg gtcaacagcc agaagacgcc acgcgggttg caccgcatcc gtgccaagat
    20341 cggcactggc gtgccggtgg gtggcgtgtt tgtcggtcgc cggtatactg gcgagattta
    20401 cacaccaaag ctggcggccc gttttcctga gcgcgactgg atactgagcc gcatcctctg
    20461 gctgtgtggc tgcgagccag gccgcaaccg tttcggcaat gtcgacagcg cgcgccgtta
    20521 catctatatc catggcaccc cagacagcga gccgatgggc gtcgcccgtt cgcacggctg
    20581 cattcgcatg cgcaacagtg agttgctctg gctctatgag caggtggcgg tggggagccg
    20641 ggttttgatc gaggagtagc agtttgccca acagttctga caaccattct gcggcattgg
    20701 gcccggtgat gctcgatgtg gccggttccg tgttgagcga ccgtgagcga gagcggctgc
    20761 gccatccaca ggtggcaggt gtgatcctgt tcagcaagaa tcatgtgaac agatcacaac
    20821 tcgttgaatt gattgatgag atacggtcga ttcgtgcaga tctgctgctg gcggtcgatc
    20881 aggaaggcgg acgcgtccag cggttcggca gcacactgac cgatctgccg gccgccggct
    20941 ggtttggcag acactatctt cagcagcccg ccgagacact gctgctgttg cgtgaaagcg
    21001 cctttctgac cggccatgag ctggcggaac tggggtttga tttcagtttt gcgccggtgg
    21061 tcgatctcga ctacggtcgc agtgcggtga ttggcagtcg agcctatcac cgtgatcctg
    21121 aactggtcac cgtgctggcc agcgcctgga tcgatggcat gcgacaggtc ggcatggcgg
    21181 ccgtgggcaa gcactttccc gggcatggct gggtcagtgc cgactcccat ctcgaactgc
    21241 cggaagatga gcggccgttg tccgacatca tcaggcagga cctgttcccc tatgccagac
    21301 tgatcgacca gttggatgcg atcatgccgg cgcatgtgcg ctatccggcg gtcgatccat
    21361 tgccggcggg cttctcggcg ttctggctgc ggcaattgct gcgtgagcgg ctcggtttcg
    21421 agggtgtcat catcagcgat gatctgtcga tgcagggtgc ggcagggatg ggtgacctga
    21481 tcggtcgtgc caacgcggcc ctcgaggcgg gttgcgagct gctgctgctc tgcaactgcg
    21541 gtgacgaggt cgatccactg ctgcggcacc tggagcagca gcgattccag ggctccacac
    21601 gtcttggacg gttgcgtcgc gccggcatcg ctgctgctgc cgaacagcag cagcgagtgc
    21661 tggcagcacg cgaagccctg gaccgactcc gattgcaaca gcgcatcaac aacggcgcct
    21721 gagcgcacgt cacgagactc aaactccatg aatgccgcag agcgttatga agagatcctc
    21781 gccagcgcgc agtgtctgta cagcactgcc gaggtggagg ccgccatcgt cgagatggcg
    21841 cggcagatca ctgcacggat cggtgcgacc aatccggtgg tctgctccgt tctgaccgga
    21901 ggcatcgtgc tgaccggcaa gctgatcacc cagttgcctt tcgcgctgga gctcgactac
    21961 ctgcatctga gccgttacca gaaccgcacc gagggtggcc tgttgcaatg gaaagcgaga
    22021 ccgaccatcg attgcaccgg gcggacggtg ctgctggtcg atgacatcct cgacgaaggc
    22081 accaccctgc aggcggcagt cgagcatttt cggcagatgg gcgcggtcga cgtgctgacc
    22141 gccgtgctga tcaacaagcg ccatcagcgc aaacatggtc tgcaggtggc cgatttcgtc
    22201 ggtctggaga ccgaagaccg ctatctgttc ggctacggac tcgactacca tggctacttc
    22261 cgcaatgtgc cgggtatcta tgggctatga tcgttgaccg tcaggggcgt cgctttcgca
    22321 aactgcggct cagcctgacg gcggcctgca actacacctg catctactgc gttccgcatg
    22381 gcaagcggct gatcaaacgc gccgatgagc tcgaatcggc ccagttcatc cgcgccgtgc
    22441 ggttgctgca actcgccacc ggcatcgaag cggtgcgcat caccggtggt gaaccgctgg
    22501 tctccccaca actcgaaccg gtcttgcagg gaattgcccg gctcggtctg accgatcttt
    22561 ccctgaccac caatggccaa ctgctgaccg accggctgcc cgtgctgctg gcggcaggca
    22621 tccggcggat caacgtcagc cttgacaccc tcgatccatt ggcatttcgt accatcagcc
    22681 gagcgggcga cctgccggcc gtgctggctg gcatcgaggc cgcgcgcgcg gcgggcatgg
    22741 cggtgcgggt caacatggtg ccgatgcggc gacgcaacca tgaccagatc gtgccgctgc
    22801 tcgattattg tctcgaacgg ggcatcgagc tgcgctacat cgagctgatg cggatgggcc
    22861 atctgctcaa cagccccgac tttgcagccg atttcgtctc gatggacagc ctgctcgacc
    22921 tgatcggcaa gcgccatccg ttccagcgca ccgatgcgcc acacgattcc accgccgtgc
    22981 gtttcgaagt gcctggccgc ggaacctttg gcatcatccc caatgaaagt gaacccttct
    23041 gccgcgcctg cacccggttg cggctctcat ccgacggctg gatctacggc tgcctgagca
    23101 acagccgccg ccaccgcatc aaccccattc tggaacagcc cgatcacgtc gccctgccgg
    23161 cgctgcaacg gctgctgacc gacgcgctgg ccgacaagca ggatcaggcc tttcagggcg
    23221 gggtcaccat catgaagctg gtcggcggct agctggccgc gctgggcgcg atgatcaaaa
    23281 tcgcgggtga gcgcgtataa tcggccgatg cacgaccttg ccccttctgt ttctgcaaga
    23341 atcattcacc tctggcgttg cctgttgctt gcgctgttct gtacggtgtt cagccgtcat
    23401 ggcgtggctg tcgacctgcc tgacccctat ctggcgcgag tcgaagtggc ggatcagtcc
    23461 actcaggcgc gcgattcggc cttgcagagg gcactgcacg aggtgctgat ccgcctctcg
    23521 ggtgatcagc aggtggtggc actgcctgcg gtgaaggagg tgctgagcaa ggccgacagc
    23581 ctgctcgatc agtatggtta cgaccgggcg ccggcacttg ccccggatgg accggcgcga
    23641 ctgctgctgg aggcacgttt caggccagag gcgatcatgg cgctgctgcg tgcggccaac
    23701 gccctctatt gggcgcccaa ccgtccgcaa ctgttgatct ggagcgtgct ggaacagaac
    23761 caggagcgtc gtctgctcaa tgccagcgag atgcaggcgc tgttgccggc actgtttact
    23821 gcagcacaac agcgtggcgt gccgctgctc tttccattga atgatctgga agatgccaca
    23881 gccctgccac tggatcagct ctggctgctc aatggcgagg cgatcactgc cgcttcgctg
    23941 cgctaccatc ccgacggtgt gctggcgggc cgcatcgagc aacggcccga tgggcgctgg
    24001 caggcacgct ggtggtttga cgacaacggt gagcgctggc agctcgatct cgatgccgat
    24061 gcgctcgatg ccctggcggc aggggccatt gatcaggtgg ccaacaactg ggccagccgt
    24121 catgcggtcc ggctgggggc gagtgcggag cagctcgagc tggagatcac cgaggtcaat
    24181 actctcgccc gctacgctgc ggtgatgtcg cacctgcaaa aactgccgtg ggcgcgccgg
    24241 gtgacactgg tcggcgtcag cgggccgatg ttgcgcttca gtctggtgag cgatgtcgac
    24301 cgcagtcagt cgcagcagtt gctgcaactg gatggcaaac tggaacaaca gagccagccg
    24361 gtggccgatg cgccgcccgg cgtggtgccg accacagcgc tgcgctaccg ctggcgtggt
    24421 ggctgattgc tcactgccat atgggcagcg catcgctgct cgctgatcac tgaaagctgc
    24481 ggagatggct gagtgttgag acagcttccc aacctgatca ccctgatgcg catcgtgctg
    24541 gtgctgccgg tgggtgtcag cgtgatccgt gaacagtacc agcaggcatt ctggctcttc
    24601 gcgttggccg gtttttcgga tggagtcgat ggtctgctgg cgcggctgtt cgactggcgg
    24661 agccgtttcg gtgccatcgc cgatccggtt gccgacaaac tgctgctggt caccaccttt
    24721 gttgccggtg ccgtcagcgg cgtctggccg tggtggttga tggtcgtggt gctgatacgc
    24781 gatctggtga tcttcagcgg ttccgttgcc taccaccttc tggtgggccc ctatcagatg
    24841 gcgcccacct ggctgggcaa actttgtacc ttctcccaga tcggcgtggt cttgctgctg
    24901 ttgcagggcc gcttctggtt gccactgccg ccatggagtc agagcctgat ggtcgccttc
    24961 gtcgccttgg tgagcatcgt cagtggtctc cactatgtgc tggtgtggag tcgaaagtac
    25021 cgtgagcaac gttttggaaa gtgaaccacg acagctggcg ttgggcattg caccgcgttt
    25081 cgaggccacc ttcgaccact atcttccggg cgaaaatgga ttgattctgg cgctgctgca
    25141 cgaggcggtg gtggctgaag cgcgctcgcc gaggctggtc tacctgtggg gggagccggg
    25201 ctgcggacgc agccatctgc tgcaggccag ttgccatctt gccgccgagt tgggccgcga
    25261 tttcttctat ctgcccttga ccgccgatct tggcgcaccg ccgcgcagtt tcgtcgagct
    25321 ggcggccgcc gagctgctct gcctcgatga catcgatcag gtcgctggcg atccgcagtg
    25381 ggaggaggcg ctctttcacc tgctgaaccg cctgcggcag aacggtcaca ccctgctggc
    25441 cactgcgtgc aatgtaccga ccggcatcgg actgaagttg cctgatctct catcgaggct
    25501 ggccgccggc gtcaccgtac agcttcagcc gctgaacgaa gccgacagac gtcggttgat
    25561 gaagcagcag gcggagcgca aggggttggc gctgagtgat gaggcggtcg aattcattct
    25621 gcggcgcagc ccgcgcgggc tgagtgatct gacggcgctg ctggagcggc tcgatcagca
    25681 ggcgtgggtc gaacagcgtc gactgacgat tcccttcatc aaacagacga tgaactggtg
    25741 agcagtcacc gcgcgccgag acgcgcggtg actgcatcag ggcgaatcaa ccttttccgc
    25801 tcgggatctg gttgaagggg agctccttgt cggtgcgcac atcctgcggc aatccaagca
    25861 cccgttcggc gatgatgttg cgcagtacct cgtcggaacc gccggcaatc cgtccggagg
    25921 gtgattgcat gaaggtctgc tggaactgcg catcgaacgg ggcgatggtc gagtcggtga
    25981 tgatgccggc catgtccatc agctccatgc cgaaggcggc cagatcctgc gacttgaagg
    26041 caccgaccag cttgccgatc gaattttccg gccctggcac ctcgccacgc gacatcgccg
    26101 agatggagcg gaagcgggtg aacttcagtc cgctcgactt gcagtaccag tcggccagct
    26161 tggcacggac cgcgccatcc tcgatggcag gacggccatt gatctgcacg cccttggcca
    26221 gatcgagcag ctcagccaca tcgaccccct gccgttcgcc gactgcgacc cgttcgttca
    26281 tcaaggtggt gagggccacc tgccagccct gaccgatctt gccgaggcgc tggctgtcgg
    26341 ggatgcgcac gttggtgaag aagacttcat tgaagccgga gttgccggtg atctgcttga
    26401 ctgggcgaat ctcgacaccg ggcgacttca tgtcgacgat gaagtaggtg agacccttgt
    26461 gcttggcgac cgaggggtcg tgccgggtga ccagaatgcc ccagtcgctg tagtgggcgc
    26521 cggaggtcca gattttctgg ccgttgatga tccagtcatc gccatcgcgt tcggcacggg
    26581 tgcggatacc ggcgaggtcg gagccggcga ccggttcgct gaagagctgg caccagatct
    26641 tctcggcctt gaccatcttc ggcaggtgct cacgcttgat ctcttcgctg gcccaggtca
    26701 tcagcgtcgg tcccgccatg ccgaggccaa tggtgaagaa tcccttgggc accagatatt
    26761 gcgactcctc ctgatcccag atcacccgct cgatggcgct ggcatcctga ccaccgtact
    26821 ccttcggcca gtgcaggcag gcccagccat tgtcatgcaa ggtgcgctgc cacttcttgg
    26881 ccagttcgac cgcttcggct tcacttttca ccagtcgctg ccaattgtct cgcggaccct
    26941 tcttgcgttc ggcgtgggcg tcgagaaagg cgcgggcctt ggcacggaat tgggcttctt
    27001 ctgggctgtc tttgaaatcc atctgtctat cctcttgatt attctgttct ttatgcggct
    27061 tgtccggcgg tcagcgcctg gatcagtttc tcttcccagc ggttctggct accaatcacc
    27121 aggctcagca gcttggcgcg gcggtagaac atgtggcagt cgaactccca ggtgaagccc
    27181 attccaccat ggatctgaat gttttcgcgg gcgcactcat acatggcctt ggtggcgctg
    27241 acgcgggcgg tggcggcggc cagtggcagc tccggcgcat tggtcgagag ggcccaggcg
    27301 ccgtaatagc cgttggaacg ggccagttcg agcgccacgt acatgtcggc catcttgtgc
    27361 ttgagcgcct ggaagctcgc aatcggtcgg ccgaaggcat agcggcccat cgcatattcg
    27421 cgggcctggt gcagtgcctg ctcggcaccg ccgatctgtt cgaaggcata gagcacggcg
    27481 gcacggttga aaacctgatc gagcagtcgg gcaccagcac cctcctgacc cagcagggtg
    27541 gccggagtgt cgttgaagac caggtgggcc tgcgagcggg tggggtcgag ggtcttcagc
    27601 gtggtgcgtt ttaccgaagg atcggtcaga tcgaccaggc agaggctggc accttggcca
    27661 ctcttggcga tgacgacggc gaagtcggcg atgtcaccgt cgatcaccgg gatctttgca
    27721 ccgctgagct tgccattgct gtagctgacg ctgtagctgg caggatcggg gttgcccgga
    27781 ccctccgcca ccgcaaaggt gccgatcagt tcgccagccg ccagcttggg caggtagtgc
    27841 tccttctgct gctgggagcc ggccagcagc agcgcctcgg tggcgacata gaccgaagag
    27901 gagaagggga tcggtgccag cgcccggccg acctcctcgg cgacgacgca gagttcgaga
    27961 tggccgagcc ccagaccacc gaactcctcg ggaatggcgg tcgaggtcca gcccatcgaa
    28021 gcgacctggt tccacagatc gcggtggaaa ggctgatccg attcgagaat cttgcgcaca
    28081 tcacgcagtg tgcagcggtc ggtcagaaac ttgttggcct gctgcttgag cagcttctgg
    28141 tcgtcggaaa attcgaaatt catgctgaga actcctgtgc ggacgggaaa gggaagtgcc
    28201 gccagcatca tgtgccgagc agcgccatgc acaccattgc aggagtggtg acgaagttcg
    28261 cacggtgatt gatctggatg caacccctcc cgtggcggtt tattgttatc ccggtagggc
    28321 agcccatggt acacagtcga cgaaaagtct ggcaagcatc ggctggataa gccttgcggg
    28381 tacggtgaac gttcggcacc acaccgtttt caccagccat caaaagagaa agccccgatc
    28441 atggatcggg gctttcgcat tgccggatcg tccctgaccg aagttcaggg acgatcgtcg
    28501 agtctgcgct caatcaatcc ttgatgtgca gttccacgcg gcggttcttg gcacgacctt
    28561 catcgctgcc attgtcggcc actggcttct cctcgccata gccagccgag gtcagacgcg
    28621 ccgcatcgac accctgagcg atcagatagt cacgcacgct cttggcgcgc gcctgcgaca
    28681 gcttcatgtt gtaggtgttg ctgccgaccg catcggtgtg accggcgacc tcaaccttct
    28741 tgccgggggt ctttttcaga gcggccgcgg cctggtcgag aatgatcttc gattcgctgg
    28801 tcagcttggc gctgttgaat tcgaagttca catccttcag cagcagcacc tgggcgacca
    28861 gacagccagt ggagtcgacc ttggcgcccg ccggggtacc ggggcactgg tcaaggcggt
    28921 cgacgacgcc gtcaccgtcg gagtcggtgt caacgacagg agggggtggt ggcggcgtcg
    28981 gttcggcaac cggctcggcg gccggtgccg gcttgctgcc caagggaatg gtcagaccga
    29041 tgccggcgaa ggtgtcccag cgggttctgc taccgcgatc tccaactggg ctggtttcgc
    29101 cttcaaggcc atcgtccaca gagcgggtgc gcagttcggt gcggatggcg gtgccgttgt
    29161 ccatcacctg ccacagaatg ccggcaccga agttgaaggc aaaatcggtg gaggattggc
    29221 cgacggtcgg tgcagcggga taggcgccgc tgctcttgac cgcgccggca ccgaccagca
    29281 ggtagggggc gattgaagag cggttgaaga agtagagcgc atcaaggccc agcccgatct
    29341 gctccagatc cttgccggtg ctgctctcac ggtcgaactt gccgtagtcg agatagcctt
    29401 cgacattgat ggagtcgctc agcgccttgc cgatctggat ctgaccagca tagtcactgt
    29461 catagctgcg cccgctgtcg agcatggctc ggtagatggt gggcgagata tagatgcggt
    29521 catcggcggc atggctgggc agggtggcga gggccgtggc ggcgcacact gctccggcgc
    29581 tgacccatgc gagtttgctc gttctgttct tcgttttgga cattgtgggt atccctggta
    29641 gttgttttgc cctgggggag tcaggcaggc ggcattatag tgtctcaatg cgcgatcgca
    29701 aaaaactgac tgctggcaag cggttgagcg ggttgctgag cggccatcca tcggctgttc
    29761 agtgctgctc gacagcgtcg gtggggctct ctgcagcgct ggattcgctg gccgaaacgg
    29821 gtggtgcgag gctgttcagg ttgccctcct cgccctgcgt ggtttcgatc gattccacat
    29881 tcgtctcgct gctcgtctca accggatcat aagccacctt gatccagctg cgctggcgcc
    29941 agcccaccga gtcgatcaga tcgagcacgc tgagcgtggt gccggcggga atgacccgaa
    30001 tcgccccggc cagccgatag aagggcggtt gcggagggtc gcgtctgaga taggcctcgg
    30061 ctgcaagggt cacggctgcg ctgccggtct ggcggaactg ctcgatcagc ctctgttgat
    30121 cccgcctggg gcccagtgag atccagccca ggctgccgca cagcggtgag cggttctggg
    30181 ccaatgtcag ttgtgcgcgc tgttctccgg cggcggcgac atgcagcagg cagcggctca
    30241 acagcgtgat gtcatgctga ttgacatggg tgcagttgta ggcgggatgg tccacatcga
    30301 cctctggctg aaaccagaac cggcgctgca gggcaaggat gtcgccgctg gcgcccacga
    30361 ccacccgccg gttcattttg ggtatgggtg gcgacagcac cttgtaggaa ggattgaagg
    30421 cgtaggcgct gacgccgtcg aacaccatcg agacatgcag agccagtcca ccgccgagcg
    30481 aatggccggt ggcgatcagt tcgacggtcg gatactgctg gcggatggcg gcaaccagcg
    30541 tatcggccag cgcatactgg ctgttgagca ggttgccgaa cagccagtca tacagcgagg
    30601 tggtgccgcg aaaggcaatg accagtttgt gtggctgacc ctgctctgcc ccgatggcgt
    30661 agagcttggc ctgcagaccg cgcccggggt caaaaaaagc acgttcactg accgtttcag
    30721 gcagtttgaa acgtcccgca ccgggcgggt cataggcatt ggcagccagc atcgcctgga
    30781 gccaggattc gctggcggtt cgatagtagc cctcgtcgta gtcgcgaccg atggtgaggt
    30841 agggttgggt gccgagcacc cccttgcagc tgttcgggtg gccggctgag cagccgaaca
    30901 gcatcagcag cagtgccagc agcggcagtc ggagcaggtt ctggatgcgg ttcaagggcg
    30961 tgatggtgcg gacaacagag cggttgtgac ccgcagtggc cggcaaaagt tccccagccg
    31021 gttgggtaga cgcttcgagt gacctaaaag tatctatggg tcaaagcgat agtgcaggcg
    31081 ttcgatgtcg agccggaagt gtgcgcccac ctgttccgcc agtgtgtcgc tgtagtagct
    31141 gcggtagtcg cggcgatcgg tggccttgcg cttgtgcggc agccgcggcg gcgtgatgcc
    31201 gatgcgctgg caggcctgtt cgaaatcggc gtcgaaccgc tcgtagcggc caatgaagtc
    31261 gacgatcagc cgaccatgca ggtcgaccag gtagtcgctt tgcagctcga tcgaggtgtc
    31321 gaggtggtac tggtagggcc gctgcggatc gagcttgaag cggatgaact cctcgaagct
    31381 ggtgacatgg gtcagcaact gggggcgttc gcgctgcaga tggtggtagg agctgacttg
    31441 cagatcccag gggttgcgga cgaaggcgaa cttgaacagt cgctggtaga gctcgcgcgg
    31501 cagcatctcc tgtgccgcga tcgccttggc gtgtcgtggc agcttgcagg cgagttcatg
    31561 gttgaacagt gcactgagcc ggctgcagag ccacatcggg tagtacttcg gatcctgcca
    31621 gcgcagccgg tcgagggcgt cgcgcacact ggtgccgccg gtcttggcga tgtgcacgaa
    31681 cagaaagttg taacggtggc tgagcagcat gtcgggtgag tgcgtgtggg gtcaaaccgt
    31741 ggcgtcgggc gattctacac cgagcgcctg caggtaacgc agcgtgctga gcgccaggct
    31801 gtattcggcg accgcctgtt gcagttgtgc ggccggaggt ggctggtcga gcaccgtggc
    31861 cattgcctcg gcgagccgtt cggcatcacc gaccggcacc agcgggccat gccggccgcc
    31921 ggccagaatc tcgcggggac cactctcgca gtcggtggcc accaccggcg tgccgagccc
    31981 catcgcctcg accagcacat tgggcgagcc ctcccaggcc gacgagagca cgaagagtcg
    32041 ggcgcgcgcc agcgcggcat aggggttggc ggtgaagcca ggcagatcga cccactcggt
    32101 cagtcccagc tcgctgatca gcgcctgcag ttgtggacga tccttgccct cgccaaagat
    32161 cagcagacgg ctggggtggg tggcccgcag ccgggcgaag gcgcgaatca gcgtggaaaa
    32221 atccttctgt cgggtgagac ggccgatgcc gacgatcacc ggcagggcgg ggtcatcgag
    32281 ccaagggtgg ctggccggtt cggcggcacg ctcgctgatc tccgggaaca gcgtggggtt
    32341 gcggatcacc cgcacgcggt cggcggtgag gccggtgaga tgacgggtgt ccgcggccac
    32401 cccctcggag accgccacca cccggtcggc atggcggtag ctgcgggcca tctggcgttg
    32461 ccgccaccac agccgcagag gatgcttgcc cttcatcgac tgttcgaggt gggtgccgag
    32521 ccggatcacc agcggaatgt cgctgcgacc ggccagtcgg cgggccagaa tcgccacgcg
    32581 gttggcgcgg tccttggccg ccagcagcgc gcgcggcggc gtccgccgca gatagcacag
    32641 cagaaagggg atcgcctgcc aggtgtgacg tgcgccgctg tggatgaccc gcacgcctgc
    32701 gggtacggca aagtgctcgc catggccctt gatcaccagc agatcgaccg gatggccgcg
    32761 ttcggcgatg ccacgggcca gcagcgtcat catccgctcg acgccaccgt tgccggagta
    32821 ggagagaaag agcgccaccg gacggtcgtg atcggtctga ctcattcgag ccactccagg
    32881 gtgttttcgg ccggggcggg cagggtgccg ggggcgtcga tgaagagccg gtgccagagg
    32941 gcgaattgca gcagcgagaa gagttcccga ctcaccggcg cgccctgctg gcgcgccttg
    33001 atcagcgcgc tcaccccctg cgggtcgaac cactcccgca cggcagcgtt gcgcggcaac
    33061 cgctcggcca tccgctccag cagtgcaccc tgcagccact cgccgaccgg tacatgaaag
    33121 ccgcgctttt tgcgttgcaa cagctcggtc ggcaggcagc tctcggccca gcgtttgaga
    33181 aagcgcttgc cgccgctggc gtcgatcttg agtgaatcgg gcagcatcag gccgaactcc
    33241 acgatgcggt ggtcgagaaa aggcacccga ccctcgacgc cgaaggccat cagcatgcgg
    33301 tcggccttga ccagcaagtt gtcgggcagg gcagtggtca gatcgacata ctgacggcgc
    33361 atcaggtcgc tccagtgggc cggggtccgt tgccaggcgg caatgaaggg ggcgcgcatg
    33421 gccgcgtggg cctgtttcag ttcggcgccg aacagccgtc ggctccagcg tgcatgccac
    33481 tgaccggtgg tgcgaaagcc gccggagccg ggacgcgcaa gctgcttgag ccagcgttcg
    33541 gcagccttgg ggcggtagcg accgtagccg gcaaagacct cgtcgccccc ctcgccgctg
    33601 aagatcacct tgagctcggc gccggccgcc tgcgccagat gggcggtcgg cagcgaggcg
    33661 tagtcgcgca tcaggtcgtc cgcggcccag acggtgtggg gcaggcgggc aaagagttcg
    33721 gcgccgctca gccgcaaggc gctgtggcgg gtgttgaact gccgcgccag ttcgctggcc
    33781 tgatcgagct catcgacacc gttgccttcg ctgaaaccga ccgagtaggt gcggggcggc
    33841 tcgtcgcgca accgcgtgag catcgccagc agaaccgacg agtcgacacc gccggagaga
    33901 aacagcccga acggcacatc cgaacgcatg tgctcgacca tcacctgacg aaagagctga
    33961 tcgaactcct cgcgggcctc atcgaagccg atctgccgcg gcgtcagcgt ggtgggtgac
    34021 cagtagcggt ggtggtgcag ttgcagcctg gcatcgattt cgatcagctc gccgggcagc
    34081 acgcgcttga tgccttcgac gatggtgtcg cggccgctgt tgaactggtt ctgcaggaat
    34141 tgcagcagcg ccggggcgtg gatggccggc gcatgtggca gcagcggcag cagcgccttc
    34201 agctccgagg cgaagatgac ccgatcgggc agttcggcgt agtagagcgg cttgatgccg
    34261 agacggtcac gccccagata gaggctgcgc cggcggccat cgtggagggc gaaggcgaac
    34321 atgccgttga tcggcgccag aaagtcgcgg ccatggcagg catagctgtg cagcagggtt
    34381 tcgcagtccg aggcggtgct gaagtggcag ccagccgccc gcagcggggc gcgcagctcc
    34441 agatagttgt agatctcgcc attggcgatc agcgcggtgt cgttgcgcgg gtcgaacagc
    34501 ggttgatggc cgccccccag gtcgatgatc gacagtcgcg tgtgcaggag gccaaggttt
    34561 tccttgatat actggccttc gtcatccggc ccccggtgtc gaagcaacgg ggcgaccgcc
    34621 gccagttcgc gctgctcgac ccgcgcttcc cgtttgatga ttccagcgat tccgcacatg
    34681 gtccgactca aggaaaaaaa gcccctgatg gtagagagtg ccctttggcc ggtcaaggca
    34741 acccgtgtcg tggggcccgt tgcgccacga tgagccgccg catcctgatc attcgcttga
    34801 gcgcgatcgg cgacatcgtg atggcctcgc cgctgattcc ggcactgcgg gcagcccatc
    34861 ccgatggctg gctggcctgg atggtcgagg agggaatgga gtcgctgatc gaggcccatc
    34921 cccagctcga tgaagtgatc atctggcccc gacggcgctg gcagcggctg tggcgcgagg
    34981 atcggcggaa ctggtggcgc gaggtccgcg ccttcgtgcg ccggttgcgt ggcagccgct
    35041 tcgatctggt gctcgatgcc caggggttgc tgaagagcgc cgcctgggct tggctgagcg
    35101 gtgcaccgcg acgaatcggt ctcaactccc gcgagggcag ccgctggctg atgcagcagg
    35161 tggtgcatga gctggatgac catccgggca tgtcgtcgga gtatcgacgg ctggcgcagt
    35221 ttctcggtct gtcgaccgac gactttcacc cctcgatcgc actgaccgaa gccgaccggg
    35281 cgcgggtgct cgaaatggtg ggaagcgaac gggccgcgcg cggttacgcg gtggccgctc
    35341 ccttcaccac ccggccgcaa aagcattggc cgcaggcgca ttggcggcag tggatcgatc
    35401 tgctggccga gcagactggt ttgccgacgc tgctgctggg tggaccgggc gaccgggcgg
    35461 cggcgcagca gatcagtgcc ggctgtcgcc atgcgccgat cgatctggtc ggccagttga
    35521 cgctgcgtca atcggcagcg gccatcgctt cggcgcggtt gctggtcggt gtcgacaccg
    35581 ggctcaccca catgggcgct ggcttccggg tgccgacggt ggcgctcttt ggttcgacgc
    35641 gcccctatct cgatgccggt ctgcccggtt ttcgggtgat ctaccatgcg ctggcctgct
    35701 cgccctgtcg tcgccacccc acctgcgccg gccgcttcga ctgcctcggc ggcatcgcgc
    35761 cggccgaggt ctgggaggcg gcgcggcagg tgatggcaga agaggcggcc cgatgaaggt
    35821 gctccatgtc gaaaccggca tgcagatcta tggcggtgcg cggcaggtgc tctatctgct
    35881 cgaagggttg caggcgcgcg gcgtcgacaa cctgctggtg acgccgcgcg gtgcggccat
    35941 cgccgaagcg gcccgtgcgc tgggggtgcc ggttcgcgaa ctgccgatgc ggggagacct
    36001 cgacatcgcc ttcgtctggc ggttgcgcca gcttctgcgg caggcacagc ccgacctgct
    36061 ccatctgcac agccggcgcg gggccgacct gtggggtgga ctggctggcc gctgggccgg
    36121 ggtgccggtg gtgctgtcaa ggcgggtcga caatccggaa ccgcgctggt gggtggcctg
    36181 caagtaccgg ctctacagcc gggtggtgac catctcgcag ggcattgccg aggtgctgaa
    36241 gcgcgagggg gtgcctgtcg aacagatcag cgtcgtgccg agcgcagtcg acacccgccg
    36301 gtttcatccg ggcgccacgg cgttgccggc ggaactcggt cttgcgctgc cgcagggggc
    36361 gccggtggcc gcgatggtgg cgcagttcat cgcgcgcaag gggcatgaac tgctgctgga
    36421 ggcgctcgag aggttgcccg agcactgtgt gcggttgcag gttctgctgt tcggtcaggg
    36481 tccgctgcgt gagcagattg cccagcagat cagcgaacgc ggcttgcagc agcgggtcca
    36541 tctgttgggt ttccatcctg cgctggagcg catcctgccg gcggtggact ttctgatcca
    36601 tccggcgctg atggaagggt tgggggtgtc gctgctcgaa gccggtgctg ccggcacgcc
    36661 agccatcgcc tcgcgcgccg gtggcatgcc ggagatggtg atcgatggcg agaccggtct
    36721 gctgatcgag ccgggtgacg tcgatgggtt ggccgtggcg atcacccgat tgaccgagag
    36781 tgacaacctg cgtcaacggc tgggtcggcg tgcgcgcgaa tgggtggaag agcgcttttc
    36841 actgacggcg atgatcgatg gaaatttcaa ggtttatcaa gagatcatga gtggtttgaa
    36901 aggtggttga ttcactgcat ctggtgggca gcagcaagct gggtggtgcc gaacgcttcg
    36961 tgttgcggct ggtgcgcgcg ctctgcgatg ccggccatga tgcacgtttg atcatcaagc
    37021 gcggttcaca actggccgag gtggcggcgc cgccggcact ggcggagctg tcgtttcgca
    37081 atgtgcgtga tccgctggcc aagtgggaga cccggcgcgc gattcgccgc accgatgccg
    37141 ccatcgtcca gacctatctc ggtcgcgcca cccggctgac gcgattgcca ctggggcgtc
    37201 agccgctgca catcgcccgt ctcggcggtt actacaagct cgatggctac cgccatgccc
    37261 acgcctggat cggcaacacc cgtggcatct gcgactacct ggtgcgcaac ggctttcctg
    37321 ccagccgggt ccatcagatc ggcaacttcg tcgagttgcc atgcgcctcc ccggccgaaa
    37381 ccttgcaacg gctgcgggcg cagtggagcg gagatcccgc tgcgctgctg ctggtcgcgg
    37441 tgggacggct ggtgccggtc aaggggctcg atcaactgct gacggccttt gcccggctgc
    37501 cggccacgct ggctgggcgg cgcctggagc tgctgctggt gggtgatggt ccgctgcgcg
    37561 agccgttgca acggcaggcc gaggcgctcg gggtggcgga gcgggtgcgc tggaccggct
    37621 ggcagaccca gcccgatccc ttctacgagc tggccgatct ggtggtcttt ccgtcgcagg
    37681 agcgcgagac cttcggcaat gtcatcatcg agagctgggc gcacggcaag ccgctggtca
    37741 cctcgctgtc gcgcggcgcg caggagttga cccggcatgg cgagaccgcc tggcaggtgc
    37801 cctgcggcga tgccgttgcg ctggccgacg gcattctgga gctgctgcgc gaccccgggc
    37861 tgcgtgccgg gctggccgag cgtggtcgag cgcagctgct ggcgcatcac accgaggcgc
    37921 aaatcatcgg ccaatacctt gagctgtata aaaaacttgc cggtggaggc tgagtggccc
    37981 actcgatcac gctgctgatg taccaccagg tcggggcgtt cgcgccgatg gcgcggcacc
    38041 gctcgaccta ttgcgaccat cggcggttcg cggcgcagat ggcctggctc aaacggtggc
    38101 gctaccaggt gctgacgctc gatcaggcag tcgcggcact ggcgggacag aggccgttgc
    38161 cagcgcgcgc ggtggtattg accttcgacg atggctacct caacttctac caatatgcct
    38221 atccgcagct gcggcgccat ggttttccgg cgaccgtcta cctgctttcc gactacattg
    38281 gtcgcacggc ggactggttc gctgccgacg gtcgcgactg tccgccgctg ctcgatcggg
    38341 cacggatcat cgagatggga gaccacggca tccagttcgg ctcgcacggt gtcagccacc
    38401 acaaactggc tgaactggat ccgcaagagg cagcgcagga gctggtgcag agcaagagcg
    38461 cgctcgaggc actgctcgag aggccgatcg accacttctg ctacccctat ggcagccaca
    38521 gcccgacggt ggtcgagcag gcgcggcagg cgggttatcg cacggcggtc acctgcgtgc
    38581 gcgcggcggc gcgaccgggt tgggactggc tgcaactgcc gcgcaaggcg atctcctatg
    38641 gcgacaccct ggccggtttc gccttcaaac tggcgttcaa gaatgagccc aagggacggc
    38701 tgccgggctt tgccgaggtt ggcatcgatt aagccggtat aatcattcag tatccagtca
    38761 gcagtcagga gatgcacgaa gatgacagag ctcgacaaac tgggagagca gctgctcgac
    38821 ccggccttca tcaagctggc gcagcggcag ttcgccaaaa gccatcccgg gtttcaggag
    38881 ctcggcatcg aggtggtgtc gatcggcgag gggcgcgcct cgatgcggtt gccctacgac
    38941 ccgaagttca tcggtgaccg caacagcggc tcgctgcatg gcggcatcat caccaccttg
    39001 atggacaccc tctgtggcct ctgcgtgatg gcgaccctgc gcacgccgac gccaatcgcc
    39061 accctcgacc tgcgcatgga ttacctgcgg ccgacccggc gcggcctcga catcattgcc
    39121 gaggcggagt gttacagccg caccaagagc gtcgccttcg tgcgcggctt cgccctgcag
    39181 ggcgacgagc gtgagccggt ggcccactgc aacgcctcct tcatgctcaa caccaagggg
    39241 gcgaacttcc cgaccagcaa cttctccagc atccacaccg atgcggaggc cggccatggc
    39301 tgactccttt tcatttctgc tggatcagca cagtgccgat caactgatca agtcgatccc
    39361 ctatgccgat tacctcggca tccgcatcga gcaggacatc aacggccaga tcatctatcg
    39421 gctgcccttt gccgaaaagc acatcggcaa tccgatgatc cgggcgatcc atggcggggt
    39481 gctggccggt ttcatggagt gtgcggccac gctggcggtg atcggcatca tcgcccatga
    39541 ccacctgccc aagtgtgtca acatgacggt ggagtatctt aagagcacgc gtccgcagga
    39601 cacctttgcc cgggtcagca tcaacaaccc gggacggcgg gtggtgcatc tgacggtctc
    39661 ctgctggcag gaagagcggc cggtcagtca ggcccatttc cgttttctgg cgacctgagg
    39721 gcctcatcag aattcatttc gctttaattt gttttaccat tattatgatt taaaagtata
    39781 tttttaaaat ttacagtttc gactttactc gatgccgaca ccgatcagca aaaaaccgac
    39841 cccaagcccg caaaagtccg tcggtggccg cgccaagagg ccgtccgggc agagcgaagt
    39901 gggtgaagcg gatggcgcat cccgtgccga gctgattgcc gcccactgga gcaagggcgc
    39961 acgcgagctg ggggccatca tgttggcctg cgcggcgctc tacctcgcca ttgctctgct
    40021 cagccacaac cagaacgatc cgggctggtc gcacaccggc aacagcaaag aggtgctcaa
    40081 tgttgccggt ctggccggtg cgatgcttgc cgacatcgcg ctgaccttcc tgggttatct
    40141 ggctggcctg ttgccgattc tgctgggaat caaggtggtt tcgctggtca ggcgtccggc
    40201 actgcaaccg ctgcggccgg cgctggtggc ggtcagggcc ggcggactgc tgctgacact
    40261 gctggcggcg gcgccgctgg tgaccctcta tgtcgccgcg cccaaggcgg gctggaccgg
    40321 cggcagcggt ggcattctcg gcatggcgat cgtcgaggca gccctgccga tcttcagtgt
    40381 cgtcggcagc accctgctct ggctgatgct gctggcgatc ggcatcaccc tgttcagtgg
    40441 actctcctgg ttctggctgt tcgatcagct gggggcagct ctgatcagat tgtcgaccgc
    40501 gttcggccgt gcgctggccg cgctgccggg ccggttgcgc ccggcgcccc gcgcggccac
    40561 ggcagcggat tcgctgaaaa atgacccgct gttcggctcg ctcgatgagc tgcccgagtt
    40621 caccttgacg ccaagatcca aggccgagaa acgggccaga aagccggtgc caccgactcc
    40681 gacagctgtc aaaacccagt ctgcaccgct caacgccgag gttgccacgg tcgccgacaa
    40741 tgtggtgcag atcagccagg cggccgagcg caaggccaga aagagtgcgg tgctggccga
    40801 tgtcgccacg gcgggcgatg aagcgctgcc gtcgctgcaa ctgctcgacc cgcccagtgc
    40861 gcagccgctg accttctata cgcccgaatc gctcgaacgc acctcgcgtc agctcgaaga
    40921 gaagctgcgt gacttcggca tcattgtcga ggtggtcggt gtcgaaccgg gcccggtgat
    40981 cacccgttac gagatccagc tggcgcccgg catcaaggcc agccgcatca ccaacctggc
    41041 ccgggatctg gcccgctcat tgacagtgat gagcgtgcgc gtggtcgagg tgatccccgg
    41101 caaggcggtg gtcggcatcg agattcccaa cgaaaagcgc aaggtggtgc ggctgcgcga
    41161 agtgctcgat acctccatct acgccgccct ggaatcaccg gtggcgctgg cgctgggcca
    41221 tgacaccagt ggtgtgccgg tggtggtcga tctggcgcgg atgccgcacc tgctggtggc
    41281 gggcaccacc ggctccggca agtcggtcgg ggtcaacgcg atgctgctca gcatgctctt
    41341 caaggccacg ccgcaggagc tgcggatgat catgatcgac ccgaagatgc tggagctgtc
    41401 gatctacaac ggcattccgc acctgctggc gccagtgatc accgacatga gcgatgccgc
    41461 caatggcctc acctggtgcg tggccgagat ggagcgacgc tatcggctga tgacccagct
    41521 cggcgtgcgc aacctgaccg gcttcaacca gaagatcagg gaggccgctg cggccgggca
    41581 accgatcgcc gatccgctgt ggcagcccaa ccccaccgga cggctgttcg acgaggatcc
    41641 ggaacagccg ccggcgctcc agccgctgcc ctacatcgtg gtggtcatcg atgagtttgc
    41701 cgacatgatc atgatcgtcg gcaagaaggt cgagcagctg atcgcccgca ttgcccagaa
    41761 ggcgcgggcg gccggcatcc atctgattct ggccacccag cggccctcgg tcgatgtgat
    41821 caccgggctg atcaaggcca acatcccgac ccgcatcgcc tttcaggtct cgtcgaagat
    41881 cgattcgcgc accatcatcg accagagtgg cgccgaacag ctgctgggcc atggcgacat
    41941 gctctacctg ccggccggta ccagcctgcc gatgcgggtc catggtgctt tcgttgccga
    42001 cgatgaagta caccgggtgg tggaagagtg gcggcggcgc ggcacgcccg actaccttga
    42061 cgagatcatc aatggcattg ctggcggtga gggtgatggc ggcggcgagg gggagcgcga
    42121 ggagagcgcc gagcaggatc cgctctacga ccaggcggtt gccttcgtca cccgcagtcg
    42181 caaggcctcg atctccgccg tgcagcggca gttgcgcatc ggctacaacc gtgccgcccg
    42241 catgatcgaa gcgatggagg aggccggcgt ggtgagccgg gccgacgtca atcagaaccg
    42301 caaagtgttg gcgccaccgc cagctggaga gtgaaatggc gatgtcgagc gcaggttgga
    42361 gtggtgcatg gctgcgctgt cttttcggtt tgtggatgtt gtggccactc tgcgccagcg
    42421 cacaggaggt ggccgatgag ctcagtcgac ttttgcgtcg caaccagacc ctgagcgccg
    42481 acttcaacca ggaggtgacc gatggcaagg ggcgccggct gcatgagacc catggacact
    42541 tctggatcaa gcggccgcaa ctgttccgct gggaggtggc cccgccctat gcccagttga
    42601 tcgtctgcga tggcaagacg ctctggcaat atgatcctga attgagccag gtggtgatca
    42661 aacccttcga tgcccgactg gcggagaccc cggcgctgct gctcagcggg gacaccgcgc
    42721 gaatcgtcga gggattccgc gtcactgcca gcgatgtgac gctgggatca caatcgggcc
    42781 gccgcttcga actgacgccc aaggccgcgg acagcctggt gtcgcagatc gagctgttct
    42841 ttgccggcaa ccagctcacc ggcatgaccc tgcgcgacag cctggggcag aacacccggc
    42901 tgcgcttctt cgcgctcacc gtcaacggcg tgatcgaagc cgacaagttc agcttcgagc
    42961 caccggccgg cgtcgacatc atccgcgacc aatgagctga cagccgccgt cgatcggtgc
    43021 aggaggcatt catgtccgag attccagcgc agcgggatcg gggctatgta ccgctggccg
    43081 agcagttgcg gccagcaacg ctcgatgaag tgatcggcca gagtcacctg cttgcgccgg
    43141 gcaaaccgct gcggctgctg ttcgagtcgg gcaagccgca ctcgatgatc ctgtggggac
    43201 cgccgggtgt cggcaagacc acgctggccc ggctgatcgc cagcgccttc gactgtcagt
    43261 tcatcgcgct gtcggcagtc tttgccggcg tcaaagagat tcgtcaggcc gtggagcagg
    43321 cgcgtgagtc gcggcagcag gaggggcggc cgacgctgct cttcatcgat gagatccacc
    43381 gtttcaacaa ggcacagcag gatgcgctgc tgccattcat cgagtcgggc ctgctcatct
    43441 tcattggtgc caccaccgag aatccgtcgt tcgaggtcaa cgcggcgctg ctgtcgcggg
    43501 cccaggtcca tccgctcaag ccgctgacca gcgatgagct gcgccagttg ctgcaacggg
    43561 catggcagcg ggccctggcg ccgctgcaat tcgatgaagc cgccagcgaa cggctggtcg
    43621 acagtgccga tggcgacgcg cgccggctgc tcaatctgct cgagcagatc agccatgcgg
    43681 cagccgtgac cggccacgaa cgggtcgatg ccgcactggt cgaggcggcg ctgagcacgg
    43741 gaccacggcg cttcgacaag ggcggcgacc acttctacga ccagatctcg gcgctgcaca
    43801 agtcggtgcg cggttcccat cccgatgccg cgctctactg gctgacccgc atgctcgatg
    43861 gtggcgccga cccccactat ctggcccggc gcatcctgcg catggcatgg gaggacattg
    43921 gcctggccga tccgcgggcg atccagatcg cccgggacgc cgccgaaacc tacgcgcgac
    43981 tcggctcgcc ggaaggggag ctggcactgg cgcaggccgt gctctatctg gcgctggccc
    44041 ccaagagcaa tgccggctat ctggcctacc agcaggcggt gcggttcgtc ggtcaggacc
    44101 agtcacgccc ggtgccgctc catctgcgca atgccccgac gccgctgatg aagcagctcg
    44161 gccatggcaa ggcctaccgt tatgcgcatg acgagcccga tgcctatgca gccggagagt
    44221 cctacctgcc cgagggcatg agtgaaccgc agtggtaccg gccggtgtcg agggggctgg
    44281 agatcaagct ggccgagaag ctggcctggc tccggcagag agacgcggca cggcgggagg
    44341 agcagtgagt ggtttggacc ggtgccgttg cgtgcgggct cagccgccgc tggccatggt
    44401 cggcagcgag gtgtgcgagc gctgccagtc gagcagtgcg tcgatctcgc tgtcgatctg
    44461 tgccatcaac cttggcaact ctgccgtgtg gctctgcttg agcgcggcct cggcggcgcg
    44521 caatgccatc ctgagtgccg gcaccccgca gtaacagcag gcgccgtgca gccgatggac
    44581 cgcttcgagc agggcggtca ggtcgccctg cgacaacagc gccgagaaac gttgctgctc
    44641 ggccgtcagt gaatcgatca actggttcag caaccgttcg gccagctctg gctgattgcc
    44701 ggccagccgg atggcctctt cacggtcgac caccccattg gcgggagcga tcggcaatgg
    44761 ctgctggatc cagcgcgaca acaatgcacg cagcgcgcgc tcgtcgatgg gtttgagcag
    44821 ggcgtcgtcg atgccggcct ggctcagggc gtggcgctcc tcgtcgagca ggtgggccga
    44881 gaccacgatg atcggcagtc gcggatcctt ggccagcggc agggtgcgca gctgccgcgc
    44941 ggtttcgatg ccatcgatgc ccggcatgcg caggtcgagc agcaccagat cgaagcgggt
    45001 atggctgacc agtgcgatgg cttcggcgcc gctgctggcg agctccacct cggcgccgag
    45061 ctgctgcagc agcagttcga gcagacgcag attggtctcg ttgtcatcca cggccaggat
    45121 gcgcggtgct gctgtcgcgg gtggctggat gggttgactg ccacgcagtt gcagcagtga
    45181 ctgggccagt ttgcggctct gtgccggctt gccgaggatc agcatcgccg ggtcgttgaa
    45241 cggcagggca tcaccgctgc gcagtgcctg acaggagagc accagcagcg gaccggcatg
    45301 gtggcggcgc acttggttgc acagcgcggc aatggtgtcg gggtgacagg gcggggtgat
    45361 gccgagcagc acggcgaacc agtgctgctg cgtcaacgcg gccatgaatg agtcggcctg
    45421 ttcatgccgc tgtggttgca gtccgagggc ttcgagctgt gcggcgatgg ccgtgcaact
    45481 ggcggcgagg gtgtcgaaca gcagcaccgg ttcgccgttg aaagggagct gtgcggcctt
    45541 gaagctggtc ggcaggccat aggtgaccgg cagcgtgaac cagaagcagc ttccctgcgc
    45601 ggagctctgc tccaggccga tggccccctc catctgctcg accagttgct tggcgatgat
    45661 cagcccgagg ccagcgccgc catgggtcgg gctctgctgc tggaaagcct cgaagatgcg
    45721 ctgctgctgt tcgggctcga tgccgatgcc ggtgtcgatc acccgcaccc gcagcagtgc
    45781 gccctgcgca tccgactgct cgacccgcac ctcgatcacc acactgccgg ccggagtgaa
    45841 cttgatcgca ttgccgacca ggttggtcag cacctgtttg agccgcaacg ggtcgccgac
    45901 cagatgaacc ggcacatcgg catggatcag cggcaccagt tcgagcggct tgtcgaaggt
    45961 gtgggtggcc agcagcagca gcgtctcttc gatcagctcg cgcagattga acggcacgcg
    46021 gtccagcacc agcttgttgg cctcgatctt ggagaagtcg aggatgtcgt tgatgatcgc
    46081 cagcaggtcg ctgctggagc gctggatcac ctgcaggtag tcgcgttgtg cttcgttcag
    46141 cggcgatttg agcagcaggt tggcaaagcc gatgatgccg ttgagcgggg tgcgcaactc
    46201 atggctggtg tcggccagaa agcgggtctt ggccagactc gccttctgcg attcccgccg
    46261 tgccaggtcg agctcgatgt tctggatctc gacggtctcc agcgtctgcc gcagatcgcg
    46321 ggtcgactgc tcgatggcct gctgggtttc gtcccgcatc tgggcgagcc gttcccccag
    46381 gtgctcgacc tgctggttga tctgcaggaa ttcacggggt gcacgccggg agagcggctc
    46441 gaccggctgt ccggcagcga gccgctcggc attgaccatc aactgttgca gcggccgcaa
    46501 cagccggcgt gtcgtgacca gcgtgatcag caggccgagc agcagcgccg caacggtggt
    46561 tgccagcaga tagaagacca cctgatactg cgccagacgg gtcggcagcc ggctgaactc
    46621 cagcaccagc cagccgctgg gctgctcgac attgcccagt ggcaacgaca accgcagcag
    46681 gtcatcctgc tgctgctgct ccaggtggtt cgacagcgtg agcggcaggc cactgcggtc
    46741 ggcactcagt ggcacgccgg cctgaatcca gggctggcgc tcggcattga acagatgcag
    46801 cgcgcgcaga tggtcgtcgc tcaacgcatc gccggccaac cgctgcaact gggccggatc
    46861 gggttggcgc agcagcagct gatagcgtcc ggccagctca tgcagcagcg attcggcatg
    46921 gcgctgcaga ttcgtctcca gctcatcgaa acgctgtgcc agtccgaaca gcgccagcgc
    46981 cacgcagacg gtcaggatcg gcgcgagcac cagggtcagc agcagtgcct ggacagtgac
    47041 ggagcgaggt gggcggggcg atgggcgcat ctgtgtcggt cacattctct taccgagcgg
    47101 tggcagggag tttatcctat gcggctgcgg gcgcgcagtc gttcacattt tcagtaaggc
    47161 aatcagatgc agccagagat tcccaccatc gaaaacctga ttggcaacac cccgctgatc
    47221 cgtctgcagc ggctgccggg cgccagctcg aacctggtgc tggccaagct ggagggacag
    47281 aatccggccg gttcggtgaa ggaccggccg gcgctcggca tgatcaaccg tgccgaggcg
    47341 cgtggcgaga tccgccccgg tgacacgctg atcgaggcca ccagcggcaa caccggcatc
    47401 gcgctggcga tggtggcggc gatcaagggc taccggatgg tgctgatcat gccggacaac
    47461 atgagcgatg agcgcaagca gtcgatgacc gcctacggtg ccgagctgat tctggtcagt
    47521 cgcgaggcgg gcatggaggg cgcgcgcgac ctggccgaac agatggccgc ggccggcgaa
    47581 ggggtggtgc tcaaccagtt tgccaacctc gacaaccccg aagcccacta ctgtggcacc
    47641 gggccggaga tctggcggca gaccgaaggc cgcatcaccc atttcgtcag cgccatgggc
    47701 accaccggca ccatcatggg ggtgtcacgc tatctga
//
LOCUS       DAORCQ010000002        45491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_379_length_45491_cov_6.211704, whole genome shotgun sequence.
ACCESSION   DAORCQ010000002 DAORCQ010000000
VERSION     DAORCQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 45491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45491
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_45491_cov_6.211704"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="gatB"
                     /locus_tag="PKI23_00215"
     CDS             <1..399
                     /gene="gatB"
                     /locus_tag="PKI23_00215"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HNN35391.1"
                     /translation="WVTGELMGRLNRENLDITASPVTAEQLASLLLRIADGTISGSSG
                     KGLLQALWSGEGDVDELIAERGLRQISDSGALDALVTAVIAAHPEQAEQFRAGKEKVL
                     GFLVGQVMRSSRGSANPTQVGDLLRHKLGA"
     gene            405..1019
                     /locus_tag="PKI23_00220"
     CDS             405..1019
                     /locus_tag="PKI23_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35392.1"
                     /translation="MNSERVPTHPQFVLGVDLDGVVADFIGGLRPIAAEWLGVPLASL
                     SEEVSYGFNEWQLESAGGYDALHRFAVKQRDLFKRLPPISGAPAVLRRLSAQDIRIRI
                     ITHRLYIPWFHREAVQQTIDWLEHHGIPYWDLCFMRDKAAVGADLYLEDNPANIAALR
                     AQGFPTIVVVNSTNHQLAPPAARNWDEVEQLVLAAFADWQSSRA"
     gene            1057..2046
                     /locus_tag="PKI23_00225"
     CDS             1057..2046
                     /locus_tag="PKI23_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNN35393.1"
                     /translation="MRRLLRTLLLTLSLGGLAPVHADEAESVTLAIQNGIAYLPLLLA
                     DEQHLIEEEAARLGLPNLKVKLLNLGSAGMIRDALIAGQVDFGAVGPPTLVNMHDKTR
                     GDIKLVSVIVSLPMTLNTTNDRVKSICQFDTGDKIALPTIKISVQAVTLQMAAKAQCG
                     DPFLLDRLTVSMAHPDGMNALLSGVVSAHFTSPPFAQAEIERGQGKVRPLLDSYSILG
                     DKSTFIVLVGSERFRARNPNLHAAVRSGIQRAKALIAQDKTAAAQLYLRRENSRETLD
                     EVLKQLDSTDLEFGEVPRGIGHYARFMHEVGSVKGRYDWKALSMPELHDQPGS"
     gene            2056..2814
                     /locus_tag="PKI23_00230"
     CDS             2056..2814
                     /locus_tag="PKI23_00230"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011266332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN35394.1"
                     /translation="MSAPLLTVRDLTLVYGAGNNAMPVLHRVSFDIRRGDRFIILGRS
                     GCGKSTLLKAIAGFLRPRSGQIELDGKSVTAPGIDRMVVWQDANQLLPWKRVLDNVAY
                     PLLRNGVAKGEARERARAFLDKVDLSRALDLYPHQLSGGMKMRVAIARALAVEPVMLL
                     LDEPFSALDALTRSRLQDELLQLQRQTGTTLLFVTHDIPEAVKLGSAILVLSPHPGQI
                     KALLDPGRVSSQELTQTISRLIHADSGDLEQESN"
     gene            2814..3677
                     /locus_tag="PKI23_00235"
     CDS             2814..3677
                     /locus_tag="PKI23_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN35395.1"
                     /translation="MNSAPRPHYIVEQQSQPVGLEAVTRSAWHWLFDRPLVRQLGVVI
                     LLCLLWEVTARQANAPLLLPTLGDTLVALRDAMVAPQGDLLAHLWESLRALCTGFAVG
                     SVIATLLVILAINLRFGETLLGTITSGFAPLPAVAIFPLALMWFGISWSSIVFITSWA
                     TLFPVAVAMFQGFRSVPDTLRQVGLNIGLHGLGYTLRILVPASLPSIMTGIRGGVANA
                     FRALIAIEMVLGAVSGNGGLGWFVMTAKQNLEMPTVYAGILAIMLIGLLFEGLFSILE
                     QCTIRRWGMVH"
     gene            4084..4392
                     /locus_tag="PKI23_00240"
     CDS             4084..4392
                     /locus_tag="PKI23_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNN35396.1"
                     /translation="MNIYVGNLPYKFSSDQLRELFAEFGEVRSANVIIDRATNQSKGF
                     GFVEMADKGQAETAIKSLNDRPIEGRNLRVNEARPRENGGGGFGGGAGAGRPPRRDRF
                     "
     gene            complement(4666..5988)
                     /gene="aroQ"
                     /locus_tag="PKI23_00245"
     CDS             complement(4666..5988)
                     /gene="aroQ"
                     /locus_tag="PKI23_00245"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma subclass chorismate mutase AroQ"
                     /protein_id="HNN35397.1"
                     /translation="MSGALPATACRPFRWLLQAALLGLLCCASLHAAEPPLRELYRLV
                     DERLELMKAVALYKRAHQLPVEDLKREQAIIDRVASDAGQLGLDPTTTRAFFQAQMEA
                     GKQLQHGWLQRWQQQPPQGLEVVDLAKEIRPRLDQINSRMLVALKAALPTLIEVDLYA
                     AHQAQFNRQVDAELLSDTDRQALLTPLLAVRATSAATAYSRLDQIIARGYLRIGTTGD
                     YPPFSEWHQADNGFAGIDIELARELAKSLGGVEARFVRTSWPQLSSDLLANRYDLAMS
                     GVSITLARQQIGLFSQRYHLGGKTPIARCSERERFSSLERIDQPGIEVIVNPGGTNQQ
                     FAETNLKRATLIRHPDNNTIFDEIIQGRADVMITDDIEVTLQSRKHPELCPTMPGSTL
                     NKSAKGILLPRDPILKAYIDTWLEQMRLDGKIDRLFIEHLGSAPVTAP"
     gene            complement(5994..6911)
                     /locus_tag="PKI23_00250"
     CDS             complement(5994..6911)
                     /locus_tag="PKI23_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN35398.1"
                     /translation="MSGTITSPPAWFSNAVAAPYEDHFIEVEGCRIHYLHWVDPERAQ
                     KPGLLFVHGGGAHAHWWSFIAPFFRDTHSVAAIDISGMGDSGHREAYPPATYARELIS
                     VCEAAGFGNDAVIVAHSFGGFGTIKTGFLYPDRLKGIMIVDTVVMSPETFEKRDETST
                     PFRPKKTYPDIETALSRFRLLPEQPCANHYIIDHIARHSLMPVEGGVMWKFDHEFNTK
                     MDYRELVEDIPRLRCKRVVIHGAESIFFNSKATAWMQQLYGPEVPFIAVPRAQHHVFL
                     DEPLAFIETVRGVLTDWDAQATPRSANAG"
     gene            complement(7031..10510)
                     /gene="mfd"
                     /locus_tag="PKI23_00255"
     CDS             complement(7031..10510)
                     /gene="mfd"
                     /locus_tag="PKI23_00255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007966.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNN35399.1"
                     /translation="MTPFSALLDRLPTRAAERLAIAPLPGALPSLLIAQLAAAHERLI
                     LVVTIDSDEAMRIEAELRFLLAAQPQVPVLTFPDRETLPYDNFSPHQEIISQRLSLLY
                     QLPQIRRGVLVIALPTLMQRLPPTHFLQGHHLQLHTGQTFSIERMRQQLDAAGYRAVE
                     NVYEHGEFAVRGAIFDLYPMGSSSPYRIELLDDEIESLRTFDAESQRTTARVDRVELL
                     PAREFPLDGAGIATFRRRWYEFFDVTPKESPIFGDINQGIAPPGVEYYLPLFFDESAT
                     LFDYLATLSLVVTIGEPIRAAQQFWQQVGNRYDELRHDREKPLLPPAELFLPPDVLTE
                     RLNQFPQLQLHGTALGSGKAEIQHDGPALPDLTIDAKAKHPLTKVQTFLHNDVSRLLF
                     VAESPGRAEALTELLKKAALRPAPSAGWEAFVTGEAPCAITVAPIERGLWLKEQGIAL
                     VTEAELFGHQLVQRRKKGRESDQSDWIFKNLAELSTGAAVVHIDHGVGRYQGLVTLSV
                     DNQVSEFLMLEYAGGDKLYVPVASLQLISRYSGADDAHLPLHRLGSDQWGKAKRKAAE
                     KIRDVAAELLDIHARRAARAGHACRLDDEDYRRFCADFPFEETPDQQRAIDAVIADML
                     AAKPMDRLICGDVGFGKTEVAMRAAFIALANHRQVAILVPTTLLAQQHFESFRDRFVD
                     WPIRIELLSRFRTAREQQAALAGMADGRVDIIIGTHKLLQPEVKFKQLGLIIIDEEHR
                     FGVRHKERLKALRAEVDILTMTATPIPRTLNMAMSGMRDISIITTAPARRLSVKTFVC
                     RKSASIIREAVTREIQRGGQLFYLHNEVKSIDKAARELQELVPQARVIIAHGQMSERE
                     LEQAMADFQHRRFNVLVCSTIIETGIDIPNANTILIERADRFGLAQLHQLRGRVGRSH
                     HQAYAYLLVPDREALTPDATKRLAAIQEADTLGAGFMLASHDLEIRGAGELLGEDQSG
                     HIGEIGFTLYMEMLERAVATLREGKVYDPEQPLASGLDVNLRGVPALIPEEYLPDVHG
                     RLVLYKRIASCRSDAALRELQVEMIDRFGLLPMQIRNLFAISALKLRCTALGIIRLEA
                     GGSGGSLEFDVKTPIDPLTLVRLVQRDPARHQLRGATTLKFSWQQANPDAQQRLLEIE
                     ALLATLAGEMPSA"
     gene            complement(10705..12693)
                     /locus_tag="PKI23_00260"
     CDS             complement(10705..12693)
                     /locus_tag="PKI23_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNN35400.1"
                     /translation="MFSKILIANRGEIAARVIRTAKSLGYRTVAVYSEADAGARHVRE
                     ADQAVLIGPSPVGQSYLSIERIIDACKRSGADAVHPGYGFLSENMAFAAACEAAGIVF
                     IGPPAPAVELMGSKRLSKIAMQAAGVPTIPGYEGADQSDETLIREARRVGFPLMVKAS
                     AGGGGRGMRLVFEEAQLAEQIRSARSEAENAFGSGELILERAIIEPRHIEIQVFADQH
                     GNVIHLGERDCSVQRRHQKVVEESPSPFMVPELRAKMGEAAVAAAKSCNYVGAGTVEF
                     LVDKHRSFYFLEMNTRLQVEHPVTELVTGTDLVEWQIRVAAGEKLPMSQEQVTLTGHA
                     IEVRLYAEEPALNFMPQTGPVLMWKAPERPGLRIDHGMVTGYEISPFYDPMVAKVITY
                     GQTRDDARRRLITGVEDTVLLGLNSNKAFLTSILRHPAFAAGEATTAFIDQHLGDEPS
                     LHPFKPGALELGLAAAIRYLQQGAKIAHRPGLAGWRNAQRQLNRYRFELGEETLEVSL
                     KVAGNLENQLSFTVSTASGESLAIDRITLDGERCHYQLNGVLNRLHYFIRDREIYLDT
                     ARGNLKLIDRTLKEPEEADLAGSGKIAAPLDGTVIAVNVKPGDRVTKGDIVVVLDAMK
                     MEHQLRSDVNGVVAAVSVSERAQVKIRQILAEVTPDEE"
     gene            complement(12763..14385)
                     /locus_tag="PKI23_00265"
     CDS             complement(12763..14385)
                     /locus_tag="PKI23_00265"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNN35401.1"
                     /translation="MPVIRTKIDSNSSTFKENAEFMLEQIEAFRAIERKMIEQANKAE
                     KKFRERGQLMPRERIEMLLDEGSPFLELSTLAGYQMDPEDPDGGGGGNIIGIGYVEGI
                     RCMISVSNSAYKGGTGGPATGKKSFRMQEIVMVNNLPLISLTESGGARLTQAADVFVD
                     GGRGFCNQSRMSAKGIPQVTVVHGNATAGGAYQPGLSDYIIMVKEQAKMFLAGPPLLK
                     AATGEVATDEELGGAEMHCSVSGCAEYLAEDDADGVRLAREVFARIPWNEQLPPRPQL
                     KYSEPLYPNEELLGIVPKDPRVPYDVREIIARIADGSDFLEFKGLWDPHTVCGHLSIE
                     GWRCGFIGNNGPITHTGSAKAAQFIQLCDQGQIPILFFQNTTGFNVGTEYEQNGIIKH
                     GSKMLQAVANTRVPKITIVVGSSYGAGNYAMCGRGLDPRFIFAWPVAHTAVMGGTQAG
                     MVMRIISEAKFVRMGVPVDEKSEQMIAKLEKETKDALDIAAGTIYGSARLWDDGIIDP
                     RDTRKLIGFLLDLCWEADHRKLQPNTFGVGRM"
     gene            complement(14456..15265)
                     /locus_tag="PKI23_00270"
     CDS             complement(14456..15265)
                     /locus_tag="PKI23_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNN35402.1"
                     /translation="MATLTLDGLVFECVIVSVSDHIMTITLNRPEKKNSISPTLGTEL
                     TYALRVAEQERDIRVVVLAANGDTFCAGGDLGTMGGGAAQQSQSTVPKIDDGRELASL
                     LRDLCKPVIVKAQGPVLAGGLLIICNATHVVAADHIFFSAPEIKRGIWPFMVMAGLFR
                     VMPRRIGLDFIMRGYKMDARTAQQHGLATEVVPAAQLDARVAELAQELADLSPAAMRL
                     GLEAFYRQELMAFDEAIPFLKEMLDKSLKTADAKEGITAFLEKRKPVWKGC"
     gene            complement(15331..17109)
                     /locus_tag="PKI23_00275"
     CDS             complement(15331..17109)
                     /locus_tag="PKI23_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNN35403.1"
                     /translation="MAGSPNDKIIIGNCSGFYGDKVSAARDLVMGGPIDVLTGDYLAE
                     LTMVILYRQRLKDGSRGWVNTHLKQMEQVMGTCIDRGIKVVTNAGGLNPRGCAEATQA
                     LADQLGLKVKVAYIEGDDLLPRIGELQQKGEQLINMDKGMPLAESTFPPVTANAYLGC
                     WGMVDALNQGADIVIAPRVTDAAVVMAPAAWKFGWKRDDWDRLAGALAAGHVIECGCQ
                     ATGGNYSFFHEIPSFVNVGYPWAEIYPDGSFTISKHPDTGGAVTVGTVTAQIMYEIRG
                     TQYLNPDVTAHFDTLRIEQRGRDRVFVSGVKGSNPPPSHKVCINTMGGYKNKMTCAIY
                     GLDAEKKANTFLDAYLTMLGGREQFDKIETQFIPTQKENPPTNEEAFSLFRINLVSQN
                     QKLVGPYLTPKMIEMGLANVPGFSTIESAGEGQALIKYWPALVSSEFISETVTFDGQQ
                     KSVAPTQQLGLPQAVVTKPGFPIPPVPTGPTAPTALGRIYAARSGDKGGSANVGIWGT
                     TPESYAFLRDFLTVERLKEIMVDVRPFEIERYEFPNLFALNFYIHDILQDGVSSSTRM
                     DPQAKTLGEYLRAKVVEIPLSIPRVC"
     gene            complement(17249..18232)
                     /gene="lipA"
                     /locus_tag="PKI23_00280"
     CDS             complement(17249..18232)
                     /gene="lipA"
                     /locus_tag="PKI23_00280"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNN35404.1"
                     /translation="MSESPPIRSRPLGVPAAAGIKLRGEEKVARIPVKVIPTDTLPRK
                     PAWIRVRLPSSGEVQRIRTILRQHRLSTVCEEAACPNLPECFGGGTATFMIMGDICTR
                     RCPFCDVAHGRPQPLDVDEPAQLAAAVAELKLRYVVITSVDRDDLRDGGGGHFAACIA
                     ALRQRSPGIEIEILVPDFRGRIAPALDALLSAPPDIFNHNLETVPRLYPSVRPGADYL
                     GSLQLLQTFKARQPAVPTKSGLMLGLGEARDELIEVMHDLRAHGVDMLTLGQYLQPSR
                     HHLPVSRFVTPDEFSELGEIARGLGFSQVASAPLVRSSYHADRQAHGEAVD"
     gene            complement(18237..18899)
                     /gene="lipB"
                     /locus_tag="PKI23_00285"
     CDS             complement(18237..18899)
                     /gene="lipB"
                     /locus_tag="PKI23_00285"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNN35405.1"
                     /translation="MNPAAGLSDLLRLRTLGLQPYDDCWQAMQRFTATRDAHTPDELW
                     IVQHPPLFTLGQAGRPEHLLDPGTIPVLQSDRGGQVTYHGPGQLLIYLLLDLQRKGIG
                     IRQLVDAIEQAVIDCLKTLGIDAATHSGAPGVYVGAEKIASLGLRVRRGCSYHGLALN
                     VAMDLSPFRRINPCGHPGLGMTQVSHFVAEATPESVTPPLCRHLMQRLAYTHGHPLTG
                     WS"
     gene            complement(18896..19165)
                     /locus_tag="PKI23_00290"
     CDS             complement(18896..19165)
                     /locus_tag="PKI23_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNN35406.1"
                     /translation="MQPTEPPRIEFPCRYPIKVMGEARDDFADFVVATVSRHAPGLLY
                     EEVSIRPSRDGNYLAVTLFIEAQGEAQLALIFGALKGDPRVRMVL"
     gene            complement(19202..19795)
                     /locus_tag="PKI23_00295"
     CDS             complement(19202..19795)
                     /locus_tag="PKI23_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNN35407.1"
                     /translation="MSSSIPLFPLKTTLFPSGRIQLQIFEPRYLEMVSFCMKQKVDFG
                     VVTIIEGEEVGELPRFHPIGTEARIVDFQQLRHNRLGLLVEGGRKFSIAHHASTPSRW
                     LQAEVEWLAPEPPLEVPAGRFEILQSTLQQLLEYPFVERLAMASDPTEARTLGWQLSQ
                     LLPLPADEKQALLTLDDPLERLLQLEQIFDALRQEAQ"
     gene            complement(19803..21098)
                     /gene="purD"
                     /locus_tag="PKI23_00300"
     CDS             complement(19803..21098)
                     /gene="purD"
                     /locus_tag="PKI23_00300"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNN35408.1"
                     /translation="MSAHTVLVIGSGGREHALAWKLAQSPQVARVLVAPGNAGTASEP
                     KVENVAIDPLEFDRLVELARQQQVRCTVVGPEVPLVAGIVDRFQAEGLACFGPTRAMA
                     QLEGSKRFAKDFLARHHIPTAAYQSFTEVAPALAYIDACGAPIVVKADGLAAGKGVIV
                     AQSVDEAKAAVQEMLAGNAFGDAGHRVVIEEFLTGEEASFIVMADGRHVIALATSQDH
                     KARDDGDLGPNTGGMGAYSPAPVITPALHQRIMAQVIQPTLAGMAAEGHPYHGFLYAG
                     LMIDAAGEPRVLEFNCRFGDPETQPILLRLQSDLFELISAALDERLDGCECRWDPRPA
                     LGVVMAAGGYPGEYATGDPIEGLAQPLPDCKVFHAGTRLDDGQVVTAGGRVLCVTALG
                     DSVAAAQALAYRNVAQIQWRGAYCRRDIGYRAVARERQP"
     gene            complement(21114..22667)
                     /gene="purH"
                     /locus_tag="PKI23_00305"
     CDS             complement(21114..22667)
                     /gene="purH"
                     /locus_tag="PKI23_00305"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNN35409.1"
                     /translation="MTPRRALLSVSDKTGLIPFAQALAARGIELLSTGGTHRALQQAG
                     IAVQEVSGYTGFPEIMGGRVKTLHPKVHGAILGRRGEDDAVAAAHGIDYIDLVVVNLY
                     PFAETIARPDCDLAMAIEQIDIGGPTMIRAAAKNHRDVAVVVEPADYERLLSELASTG
                     SLSAATRLRLAGVAFAHTARYDDAIANYLGRLDEVDGECFPRSLNLQFDKVEPMRYGE
                     NPHQRAAFYRERDSREAGIATARQLQGKALSYNNVADTDAALECVKSFSEPACVIVKH
                     ANPCGVAVAATPLAAYELAYRTDPTSAFGGIIAFNRPLDLACASAIIERQFVEVIIAP
                     AVSEEALPVLASKPNVRLLVTGAWGEPQPALDYKRVQGGLLVQERDLGQVSAAQLRQV
                     TRRAPTAEELEDLLFAWRVAKFVKSNAIIYARGGSTIGIGAGQMSRLYSARIAGIKAA
                     DEGLTVAGSVMASDAFFPFRDGIDTAAAAGIRAVIQPGGSLRDAEVIAAADEADMAML
                     FTGMRHFRH"
     gene            complement(22887..23612)
                     /locus_tag="PKI23_00310"
     CDS             complement(22887..23612)
                     /locus_tag="PKI23_00310"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNN35410.1"
                     /translation="MQLSPADIASQAEQLAAKSPREILRHAFARFDAIAISFSGAEDV
                     VLIDMAVKLNKAVQVFSIDTGRLHPETYRFIDRVREHYQIPIELLFPDADQVRQLVNA
                     KGLFSFYRDGHQECCQIRKTNPLRAKLATLDGWITGQRRDQSPSTRQQIPVMELDPAF
                     SSEARQLVKFNPLANWSSQQVWDYIRSFDLPYNPLHERGFVSIGCEPCTRPVLPGQHE
                     REGRWWWEEAAHKECGLHVINLK"
     gene            23854..24471
                     /gene="thrH"
                     /locus_tag="PKI23_00315"
     CDS             23854..24471
                     /gene="thrH"
                     /locus_tag="PKI23_00315"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNN35411.1"
                     /translation="MEIACLDLEGVLVPEIWINFADETGIAELRATTRDIPDYDRLMR
                     QRLALLDQHGFGLPDIQRVIDRMGPLPGALEFVDWLRERFQVVILSDTFYEFAQPLMR
                     QLGWPALLCHRLQVNEQGRVVDYLLRQKDPKRHAVEAFKSLNFRVISAGDSYNDTTML
                     AASDAGILFRAPANVVAEFPQFPTAREYDELKERFLAASDRKLSR"
     gene            complement(24490..24756)
                     /locus_tag="PKI23_00320"
     CDS             complement(24490..24756)
                     /locus_tag="PKI23_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNN35412.1"
                     /translation="MKVVSFYEMAPDALPKIRAHYPAHRARLDAFHARGIVLAAGPFG
                     DPTEGAMGIFASREGAEEFIQGDPFVTEGLVSRWRLLAWNALFI"
     gene            24933..25361
                     /locus_tag="PKI23_00325"
     CDS             24933..25361
                     /locus_tag="PKI23_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HNN35413.1"
                     /translation="MVKPIRFTDRFAVHPQIRPEALEEVAALGYRRVINNRPDGEEPG
                     QPRSAELEAKAKALGLEYHHLPITLPGLHEAQIEQMEALVADQAPVFAFCRTGTRCAI
                     LHAIMAVRAGRPLAELLAEAARNGYDLTAQEPLIRHFGDQ"
     gene            complement(25395..26219)
                     /locus_tag="PKI23_00330"
     CDS             complement(25395..26219)
                     /locus_tag="PKI23_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN35414.1"
                     /translation="MIKSVFSANLFAGKVAVVTGGGSGIGLRTARELAQLGATVVIGG
                     RNEEKLKSSVETIRAEGGTVHAFPLNIREPEQVKSFFEQVVATCGTLDFLVNNAGGQF
                     PSPAEKISPKGWRAVVDTNLNGTFFVTQEAFNQAFKEKGGAIVNIIANMWNGFPNMCH
                     TGAARAGVDNLTKTLAVEWGRYGVRINSVAPGAIYSSGIKTYDPEFQKFFLQAGKHNQ
                     TYRLGSEAEVAAAICFLLSPAANFISGETLKVDGAESLPHHSFAPVDHGLLPPWDE"
     gene            26417..28240
                     /gene="typA"
                     /locus_tag="PKI23_00335"
     CDS             26417..28240
                     /gene="typA"
                     /locus_tag="PKI23_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNN35415.1"
                     /translation="MTQQIRNIAIIAHVDHGKTTLVDRLLQQSGTLKRGEEQIERALD
                     SNELEKERGITILAKNTAIAWQGNRINIVDTPGHADFGGEVERVLSMVDSVLLLVDAV
                     DGPMPQTRFVTQKAFAQKLNPIVVVNKVDRPGARPHWAVDQVFDLFINLGATEDQLDF
                     PVVYCSALNGIAGLEPDALAEDMAPLFETIMARVPAPQVDLDGPLQMRISTLDYSSYL
                     GIIGIGRINRGRARRGQPVTVVAADGSRRAGKLATIMGYMGLGRVEVEEAEAGDIVCV
                     TGFDELNISDTLCDPALPEALPALSVEEPTVSMTFQVNTSPFAGREGKYVTSRKIKER
                     LEEELKHNVALRVEATDSADRFRVSGRGELHLGILIETMRREGYELGVSKPEVIIKVE
                     NGERFEPFEELVVDIEETHQGAIMETLGARRGELQEMVPDGKGRVRLRYRIPARALIG
                     FRSDFMTMTSGSGIMSHNFLAYGKAHPGDVGGRNNGVLIANGQGKALGFALFNLQNRG
                     RLFIGPGEEVYEGMVIGIHSRDNDLVVNPLKGKQLTNIRAAGSDENIVLTPPIRMTLE
                     RALEFIAEDELVEVTPGSIRLRKKQLKESDRRRDSRSASSE"
     gene            complement(28252..29091)
                     /locus_tag="PKI23_00340"
     CDS             complement(28252..29091)
                     /locus_tag="PKI23_00340"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN35416.1"
                     /translation="MSEPLRVLVAGCGDVGSQAATLLAVRGAQVWGLRRQSSLLPTGV
                     RPCSADLGDPATLRALPAVDYLLFTAAAEHSSEAGYRALYVEGLRNLLAALPAAPRRL
                     LFTSSTGVYGQSQGEWVDEESPTEPQRFSGRIMLEAERLALASGLPTTLVRLGGIYGP
                     GRNHLIERVRSGQGSPLSPTLYGNRIHSADAARLLDFLVERDRQGLPVAQCYLGVDDE
                     PAPLHEVEAFIAGLLGVTLQRRGESQRLGGSKRCSNQRLRRSGFELRYPSYRDGYRAL
                     LSQ"
     gene            29169..31256
                     /gene="recG"
                     /locus_tag="PKI23_00345"
     CDS             29169..31256
                     /gene="recG"
                     /locus_tag="PKI23_00345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNN35417.1"
                     /translation="MTQAVDFWQAPLTRLSGVGPQLAARLARLDLHRIEDLLFHLPHR
                     YQDRTRLTPIGQLRPGHEALVEGEVQFSEIAPGRRRTLLCRISDGRAALTLRFFHFSP
                     KQQQWLRAGLWLRCHGEVRSGMAGLELVHPELELLSAPGQPPLSPHLTPIYPATDGLT
                     QTRLRNLIDQLLLRMQQLGAPPDYLAGLTAQQGDDWSELLRAVHHPPPSQDAALRQQQ
                     LDRLRRHLAREELVAHQLTLGRLKQQAQRHRAPPLPVEQSRAAAERLAAALPFRLTAA
                     QQRVIAEIGADLQQRRPMQRLVQGDVGSGKTLVAAFAALQALESGHQVALMAPTELLA
                     RQHAENFDRWLAPLGWEVALLSGSQKGKVRADQAQAVASGRAMLAVGTHALFQTSVQF
                     QRLGLVIIDEQHRFGVHQRLALRNKGGEQLWPHQLIMTATPIPRTLAMSAYADLDLSL
                     IDQLPPGRQPITTSVINAARRDQVIERIRLNCAQGRQVYWVCTLIDASEQIEAQAAAD
                     TAAQLAAALPQVRVALLHGRMKEAEKQQTMARFKAAEIDLLVATTVIEVGVDVPNASL
                     MIIENAERLGLSQLHQLRGRVGRGQIDSHCLLLYRAPLSPNGRARLAVMRESQDGFRI
                     AEEDLRLRGPGELLGTRQTGLMQFGQADLGRDADLLPEVKQWAERLPAHRPELIDPLI
                     ARWFAHKEGFITV"
     gene            31363..32703
                     /locus_tag="PKI23_00350"
     CDS             31363..32703
                     /locus_tag="PKI23_00350"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNN35418.1"
                     /translation="MSFATAVASDPLMTPDQPIEAAMRLRPLLLERGGKQKLMLLTGA
                     DRMVQLEPLSRLLGADLRAARPGDQAHWSAKWLAQGADDRLPCIIDAALIDAPRGFIE
                     IDGDGQSIPLSEESWRGWCDGARIAEVSLPVDELEAAYLHAEQARIEAEREEDGGESF
                     SAQRIRARLSETLSIPPLSEVARRLIQLQANPDATGEDLARIVECDASLAAQVVGWAS
                     SPFYGKRGGVRTVREAIIRVLGFDLVLNLAIGLAMKRSLGQPQDAPQGLTSYARQSIY
                     LAASTALLSTMMPRNQRPTYGAAYLCGLLHNYGHLLLGCLFPPHFSLICRHQELNPRL
                     PRHWMEQQLLGVTREQISCWMFRQWSLPDEVVSAIQSQHGGWGESRHACLGQLLYLCS
                     RLLRQRGVGDLPCDLIDPQLYVALQIDEAEVALRLDELIENSETLDQLAQSLGR"
     gene            complement(32728..33129)
                     /locus_tag="PKI23_00355"
     CDS             complement(32728..33129)
                     /locus_tag="PKI23_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNN35419.1"
                     /translation="MCPRISSAHSARWARRCSRWSSSEFVAARPTFGNCQATHPVTKS
                     ELIREVAERIDMSPERTACVIDAILDQITRALQRGESVTLTGFGSFTPRRRNARTGRN
                     PRTGAALEIPAHTTVLFRPGKQLKAWMAGNG"
     gene            complement(33059..33232)
                     /locus_tag="PKI23_00360"
     CDS             complement(33059..33232)
                     /locus_tag="PKI23_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNN35420.1"
                     /translation="MSDWKKYECIVCGWVYDEALGCPEEGIEPGTRWEDVPEDFICPL
                     CAVGKEMFEMVEF"
     gene            33458..34324
                     /gene="ubiA"
                     /locus_tag="PKI23_00365"
     CDS             33458..34324
                     /gene="ubiA"
                     /locus_tag="PKI23_00365"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNN35421.1"
                     /translation="MFPRRRPLHAFIQLTRLDRPIGIYLLLWPVAWALWLAADGWPGW
                     RHLLVFTLGTVVMRSLGCIINDWADRDLDGHVARTRQRPLVTGLASSRDALGYGLGLL
                     LLALLLVLLTNRLTMLLAIGALLLAALYPFMKRHTHLPQLVLGAAFAWGVPMVFAAVQ
                     ERVPATAWLIYLTTLLWTVAYDTLYAMVDREDDLKIGIKSTAILFGSADRAMVALLQL
                     LSLLGWALIGVNFDLGLCYALAVVAAALLFGHQQWLIRDRAPAACLAAFLHNQWVGAV
                     LFVGIVLDQLLR"
     gene            34335..35471
                     /locus_tag="PKI23_00370"
     CDS             34335..35471
                     /locus_tag="PKI23_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNN35422.1"
                     /translation="MEVRLSHRIDEIDPARWDAVVGRDYPFLRHPFLQALEASGAVSV
                     ETGWQPLHLTLWEQERLLGLMPLYLKSHSYGEYVFDWSWADAYRRHGLDYYPKLVTAI
                     PFTPASGPRLRLADGLDSAEAIGRVSMTLHNLAERLGASSWHGLFFEPAQLPHWLDQG
                     HLLRKGCQFHWHNPGFRDFADFLDGFNSRKRKAVRRERRLVEEQGLRLERLTGDQVSE
                     ALWQRFYACYQSTYRQRGQRGYLNLDFFLRLAQQMGESLLLVVARREAEVVAAALSLH
                     DDETLYGRHWGALEQHDQLHFEACYYQGIEFCIERGLKRFDPGTQGEHKIQRGFAPVE
                     THSLHWIADGRFRRAIADFVAEEALGIDRYRQQAATLLPFRQGD"
     gene            complement(35487..36731)
                     /locus_tag="PKI23_00375"
     CDS             complement(35487..36731)
                     /locus_tag="PKI23_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN35423.1"
                     /translation="MFYGWILLAALSLIYFLSLGSIYYGFSVVIPEVVASMGWSRTEV
                     SAGFSIINLMLGCAPFVADWLIRRRGSRFCIALGGAVAAVGAAVVYLCSSLPLFYLGT
                     AVLGLGMTIQTVLPGTQLLTRWFSRRRALAMGVFMAASGLGGFVAAPSFAWIIAASHN
                     WRLVFVAMALCTLAASLLALVSVKESPEEVGEVPDGRKATQANAAATTVKRSHVHQTQ
                     YPWTFREALRTYPFWAIVISGAFVVIGNTIVNSQAMLHLQDQGLSIVLSATVLGLIGL
                     FNTGGRLLTGVLGDRYDPRYLLGAGLLVQLLGIVVLDQTTQPWIAYLFAVLFGIGYGM
                     ASVASFPLIANYFGASSYARLFSVRSLVATALGAVGPVAAAMAHDSLHSYRPVFLGFA
                     AIALLMVVMAITMRPPLPPTAD"
     gene            complement(36918..37178)
                     /locus_tag="PKI23_00380"
     CDS             complement(36918..37178)
                     /locus_tag="PKI23_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNN35424.1"
                     /translation="MPTYDYICDSNGQVVEVKHAMSSEVHSWGELCELAAIDAQGTPL
                     DAPVRRLATGGQVMSGRSSAKAEPAAAVPAATGHRCAAGCWH"
     gene            37349..37843
                     /locus_tag="PKI23_00385"
     CDS             37349..37843
                     /locus_tag="PKI23_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNN35425.1"
                     /translation="MKLIVRRFMALLLLALLCGIAGCSLLFSKPKPPEVFISSIHIES
                     LALAEQVLLFRLRLHNPNDYPVPIRELTYRVELNDQLFAEGGSAEAVTLPALGDSEIE
                     LRARVRSTQLLTQLSRMALRGQSSVRYRLNGRLTLTRGHLGVDFRQEGSLSLPDLRSL
                     PRRR"
     gene            complement(37818..38630)
                     /locus_tag="PKI23_00390"
     CDS             complement(37818..38630)
                     /locus_tag="PKI23_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN35426.1"
                     /translation="MAETLFMIHGMWGGPWYWAGYRRLFEAAGHRTFAATLPFHDIAP
                     GQPPDPRLGCASLLDYADALARQIGELEQKPVLIGHSMGGLLAQMLASRGLARAVVLL
                     APASPAGILALSPSVIRSFWRIATRWGFWQRPTRQGFDEAVYSMLHRLPPTLQRETYD
                     RFVHESGRATFEIGLWPLDLRHASRLDASRIDCPMLIIAGREDRITPVRVVRQVAQRY
                     RRVATYLELPNHAHWLVAEPGWEQIVAAIDHWLHAVGPAHRTIALISAGAAT"
     gene            complement(38686..41331)
                     /locus_tag="PKI23_00395"
     CDS             complement(38686..41331)
                     /locus_tag="PKI23_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN35427.1"
                     /translation="MNKESFIIAIAVAALTLMFWAGMNRPDTEPAWPKTIQGFSFAPF
                     RADQDPTDQRYPSIEQIDEDLALLSGRTHAVRTYTVEGSLGEIPDLAKAHKINVTLGG
                     WVSGDAEATQRELKRLIDIANRSTNVVRLMVGNESLLRGEVSIEQLTAYLDQVRGRTD
                     IPISTAEPWHIWLKYPELADHVDFITIHLLPYWEGIALEHAVDYVKEQTDKIKAAFPN
                     KPVIIGEVGWPSNGRTRQSAVASSANEAIFLRRFLALAKEENYTYYLMEAFDQPWKHG
                     VESGVGAYWGVYNVWREPKFPFSEPIVEIPNWPMLAGISIVVALVTFGLLLIDSRFLR
                     SRGRSFLAIVAYSAATAVVWIIYDYSNQYQTLTSVVVGILLVLGMIGVIVVLFAEAHE
                     WAEALWLQQHRRLFKPTEVDEALLPMVSIHVPAYNEPAPMMIETLDALAKLDYPRFEV
                     LVIDNNTKDPAVWQPVAAHCEQLGERFRFFHVDPLAGFKAGALNYALRETAAEAEVVA
                     VIDSDYKVDPRWLRDLVPQLLKEKIAIVQAPQDYRDAHENAFKAMIHAEYRGFFYIGM
                     ITRNERNAIIQHGTMTMVRKDALQQVDGWAEWCITEDAELGLRIFEQGLEATYIPKSY
                     GRGLMPDTFTDFKKQRFRWAFGSIQIMRGHLRQLLRGEGRLTPGQRYHFLAGWLPWIA
                     DGINVIFNLAALGWSAAMLLAPRTIDPPLLIFSVLPLTLFVFKLGKLIYLYRTRVGTS
                     LRQTVAAAVAGLALTHTIGMAVLTGFFVKDKPFFRTPKQADAQALLKALSAAREESLM
                     LTALLLASGAIISQFGTETLDLLAWAIMLLMQSIPYACSLLLSLISALPKLPAGLIGQ
                     SDSMRDAAQQLLAPLPDAGTKLTTQ"
     gene            complement(41456..43417)
                     /locus_tag="PKI23_00400"
     CDS             complement(41456..43417)
                     /locus_tag="PKI23_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta (1-6) glucans synthase"
                     /protein_id="HNN35428.1"
                     /translation="MTRLSGPIAYLLALLLLVALWSLLGQPRAVQPAGLTAGQKLQCV
                     SYAPFEKAQSPFDLDDGLTISERRIEQDLALLSQRFECVRTYSVTGLEAVPKLAGKFG
                     LKVLLGAWVSSDPVATRKELERVIALAKQHPAVVRAVIVGNETLLRKEVTGAQLASYL
                     RQVKAALPEVPVTYADVWEFWLKHPEVAPATDFVTIHILPYWEDDPASIDTAIAHISQ
                     VYGEVTEQIPGKRLLIGETGWPSAGRMREGALPSLTNQARFIRGFVQLAEQQGWQYNL
                     IEAFDQPWKRINEGAAGGYWGLYDTHRADKGVLSGAVSHFAQWRTLLLLSAFAVALCL
                     PLLIQPRRLPTARWALVIATLVAGALLLAMQADQFSQSARNGWEYAWAAFIMALSLGV
                     YLLTIQALTGGQIAPPLPIATLLDPTAHRTRFDLPWLNGWLRAAILFCALVENTGLVF
                     NARYLSFNSQAFVLPALAYLWLFRRPVTSANASLIERHIALFLTFSAGFILLNETPLN
                     LQADLWLLVTLLLAWPLWRESPGQRLSALRPMLIAGVTAYAAAASVRYGLMESVEMVN
                     RCAEAAHGLSCTLRTGMGLIIHWRLLGWLSLGIALLAVTRRSRRLGILALMLGILGLV
                     LYNAAQATFALVLAFLLLSWLAARSPSTR"
     gene            complement(43494..>45491)
                     /locus_tag="PKI23_00405"
     CDS             complement(43494..>45491)
                     /locus_tag="PKI23_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN35429.1"
                     /translation="FLMTTLFYLCAPLRLLWTVALNLLLSLGYLWIVGHKPDVMAYDS
                     IANSLKLAAFNLAISGYLWQHQRAVRINFWKDLDLSRVNAALQREVDKRIDSEQMFRA
                     LTENSPSMVTILDAHHQVRYLSPSTYRALGWSKPTQNTLSIQGIVHPEDLALFMSQLN
                     LARQQSGTVHRFSNVRIHHRQGHWLDVEGYIVPMLDVPGVQGIVVNYHDVSELKAAQA
                     DLTQLAFFDSLTRLPNRQLFRDRLEQTLQSCRRHHETAALLFIDLDRFKEVNDTLGHE
                     AGDQLLQSVAQRLTAHVRREDSVARLGGDEFTILLHKADIKAAEKVAGNILDALRQPV
                     SINGHAVTVGASIGIAMIPADGQESTELMRYADLAMYSIKEQNRNGYRFFAEEMNRSA
                     LARMRAQDELKSALADQQFQLHYQPQFTLADQRLIGFEALSRWSHPTEGLLGPERFLG
                     TLEQSGLIIEHGHWTVAEAIAQGAWLRNSEPGKAYRMAINLSVRQLLDPALIDTLAAS
                     LDRHRFPASLLTIEIHESFLSQQLEAANRVLWQLRRLGVTIAIDDFGTGYSSLHLLKE
                     LPIDSVKIDRRFIQGIPNREEDVAITEAVIVMAHRLGLTVAAEGVESVQQLRFLLDQG
                     CDLAQGHLFDAAIAAEQLPALIQELSARKSMLPRPQLVFDV"
BASE COUNT         7721 a        14208 c        15249 g         8313 t
ORIGIN      
        1 tgggtcaccg gcgagttgat ggggcgactc aaccgcgaga acctcgacat caccgccagt
       61 ccggtcactg ccgaacagtt ggcaagtctg ttgctgcgca ttgccgatgg caccatttcc
      121 ggcagcagtg gcaaggggct gctgcaggcg ctgtggagtg gcgaaggcga tgtcgacgag
      181 ctgattgccg aacgcggact caggcagatc tccgacagcg gtgcgctcga tgcgctggtc
      241 actgcggtga tcgcagccca tccggagcag gccgaacagt tccgtgccgg caaggagaag
      301 gtgctcggtt ttctggtggg gcaggtgatg agaagttcgc gcggcagcgc caatccaacc
      361 caggtcggcg atctgctgcg gcacaaattg ggtgcatgac agcgatgaac agtgagcgcg
      421 tgcccaccca tccccagttc gtgctggggg tcgacctcga tggcgtggtc gccgacttca
      481 tcggcgggct gcggcccatc gccgccgaat ggctgggtgt accactggcg tcgctgagcg
      541 aggaagtcag ctatgggttc aacgaatggc agctggagtc cgccggcggc tacgacgcgc
      601 tgcaccgttt tgccgtcaag cagcgcgacc tgttcaagcg gttgccaccg atcagcggtg
      661 cgccggccgt gctgcgccgt ctctcggcac aggatatccg catccgcatc atcacccatc
      721 gcctctacat tccctggttc catcgtgaag ccgtacagca gaccatcgac tggctcgaac
      781 accacggcat tccctactgg gatctctgct tcatgcgtga caaggcagcg gtcggcgctg
      841 acctctatct ggaggacaat cccgccaaca tcgccgcact gcgcgcccag ggttttccca
      901 ccatcgtcgt cgtcaactcc accaaccacc agttggcacc gccagcagcc cgcaactggg
      961 atgaggtgga gcagttggtg ctggcagcct tcgccgactg gcaatcctct cgcgcatgac
     1021 cccggacgag gtggcaactt gacccggaga cggtcgatgc gaagactcct gcgcaccctg
     1081 ctgctgacat tgtcgctggg cggcctggca ccggtgcatg ccgatgaggc cgagagcgtc
     1141 acgctggcga tccagaacgg cattgcctat ctgccactgc tgctggccga tgaacagcat
     1201 ctgatcgaag aggaggcggc ccggctgggt ctgcccaacc tcaaggtcaa gctgctcaat
     1261 ctcggctccg cgggcatgat ccgcgatgca ctgattgccg ggcaggtcga tttcggcgca
     1321 gtgggtccac ccacgctggt caacatgcat gacaagaccc gaggtgacat caaactggtc
     1381 tcggtcatcg tcagcctgcc gatgacgctc aacaccacca acgaccgcgt caaatcgatc
     1441 tgccagttcg ataccggtga caagatcgcg ctgcccacca tcaagatctc ggtgcaggcg
     1501 gtgacactgc agatggcggc caaggcgcag tgtggcgatc cgtttctgct cgatcgtctg
     1561 accgtatcga tggcccatcc cgacggcatg aacgcactgc tgtccggtgt ggtctcggct
     1621 cacttcactt cgccgccctt tgcgcaggcc gagatcgagc gcggccaagg caaggtcaga
     1681 ccgctgctcg actcctacag cattctgggt gacaagagca ccttcatcgt gctggtgggg
     1741 tccgagcgct tccgcgcgcg caatcccaac ctccatgccg ccgtgcgcag tggcattcag
     1801 cgtgcgaagg cgctgatcgc acaggataaa accgccgccg cccaactcta tctgcgccgc
     1861 gaaaacagcc gtgaaaccct cgatgaggtg ctgaagcagc tcgattcgac cgacctcgag
     1921 tttggcgaag tgccgcgtgg catcggtcac tatgcccgct tcatgcatga ggttggcagt
     1981 gtcaagggcc gctatgactg gaaggcgctg agcatgcccg aactgcacga tcagccgggc
     2041 agctgagggg cggccatgag cgcgccgctg ctgacagtgc gtgatctgac cctcgtgtat
     2101 ggggctggaa acaatgcgat gccggtgctg catcgggtca gcttcgacat ccggcgtggc
     2161 gaccgcttca tcatcctcgg ccgttccggc tgtggaaaat cgaccttgct caaggcgatt
     2221 gccggttttt tgcgccccag atcggggcag atcgagctcg atggcaagtc ggtcaccgcg
     2281 cccggcatcg accgcatggt ggtctggcag gatgccaacc agttgctgcc gtggaagcgg
     2341 gttctggaca atgtcgccta cccgctgctg cgcaacggtg tggccaaggg cgaggcccgc
     2401 gagcgggcca gggcctttct ggacaaggtc gacctgagcc gtgcgctcga cctctatccg
     2461 catcagctct ccggcggcat gaagatgcgg gtcgccattg cccgcgcgct ggcggtcgag
     2521 ccggtcatgc tgctgctcga tgagcccttc tcggcgctcg atgcgctgac ccgcagccgc
     2581 ctgcaggatg aactgctgca actgcaacgg cagaccggca ccacgctgct gttcgtcacc
     2641 cacgacattc ccgaggcggt caagctgggc agcgccatct tggtgctctc accccacccc
     2701 gggcagatca aggcgctgct cgaccccggc agagtttcat ctcaggagtt gacccagacc
     2761 atctcccggc tgatccatgc cgacagcggt gatctggaac aggagtccaa ctgatgaatt
     2821 cggctccacg cccgcactac atcgtcgagc agcagagtca gcccgtcggg ctcgaggccg
     2881 taacccggag cgcctggcac tggctgttcg atcgcccact ggttcgacag cttggagtgg
     2941 tgattctgct ctgcctgctg tgggaggtca ccgcccgcca ggcgaatgcg ccgctgctgc
     3001 tccccaccct cggcgacacc ctcgtggccc tgcgcgatgc gatggtggct ccgcaaggcg
     3061 atcttctggc ccatctgtgg gagtcgctgc gggcgctttg caccggtttt gcagtcggca
     3121 gcgtgatcgc cacgctgctg gtgatcctgg cgatcaatct gcgtttcggc gaaaccttgc
     3181 tcggcaccat cacctccggt tttgcgccgc tgccggcagt ggcgatcttt ccgctggcgc
     3241 tgatgtggtt cggcatctcc tggtcatcga tcgtcttcat cacctcctgg gcaaccctgt
     3301 ttccggtggc ggtggcgatg tttcaggggt ttcgcagtgt gcctgacacc ttgcggcagg
     3361 tcggactcaa catcgggctg catgggctgg gttataccct gcgcattctg gtgcccgcct
     3421 cactgccctc gatcatgacc ggcatccgtg gcggtgtggc caatgccttc cgtgccctga
     3481 tcgcgatcga gatggtgctg ggcgcggtct ccggcaatgg cgggttgggc tggttcgtca
     3541 tgactgcgaa acagaatctg gagatgccca ccgtctatgc cggaatcctc gccatcatgc
     3601 tcatcggctt gctgttcgaa gggctctttt ccattctgga gcaatgcacc attcgtcgct
     3661 ggggcatggt gcactgagcc tgccgtgctg cggattgtca tgaaaaaata gccgaagagg
     3721 gttgcaaggt ctgtcaatca tccatataat ttggcgtcat ggacaatccg cgactttgaa
     3781 tcatgaagag aagaccgccc agtcagcgat agaccctcgt tctcatgccg cgatcaatgg
     3841 tcgtggaggc tctgtgttcc cacctgatca gttcaggttc cggccctctc gtcacctcga
     3901 cagcatcgcc agcgaagtgg atcaaaatcc tgtcaagtcg gtctttcagg cgcattcaac
     3961 ctgcggaatc tgttccaaaa gaattggcca tgaccggcca attgccatga tccggtaagt
     4021 gcgctcgacc ggatggtggt gtacttgaag ctcaaacgca cgtttttttg atgagaattt
     4081 tgcatgaata tctacgttgg aaacctccct tataaattca gcagcgatca gttgcgtgaa
     4141 ctgtttgctg aatttggtga ggtgcggtcc gcaaatgtga tcattgaccg ggccacgaac
     4201 cagtcgaaag gctttggttt cgtggagatg gctgataaag gccaagccga aaccgccatc
     4261 aagtcgctca atgatcgtcc gatcgaaggc cgcaacctgc gcgtcaacga agcccgtccg
     4321 cgtgaaaacg gcggtggcgg ctttggcggg ggcgcgggcg ctggccgtcc accccgtcgg
     4381 gaccgtttct gagcataccc gtcctgcttc gccgtcggca gggttcgatg gtgaagcaga
     4441 tcagttcagt ccgcagggca ctttaagctc aaaggagccc tgcggctgct gacgaggtct
     4501 gacgtggccc aatttcgggc agacatcttt tttgagtgcg aatttcggct cgaattcggt
     4561 gtcatccatt cgtgcgcttc atgtgctgcg tcacccagtg cagatcagcc ttgtgctgtc
     4621 tgttgctgcc tgaatcttgc gcaagaataa gaaaaagccc ctccatcagg gcgctgtgac
     4681 tggtgcactg cccagatgtt cgatgaacag acggtcgatc ttgccgtcca gccgcatctg
     4741 ctcgagccaa gtgtcgatat aggccttcag gattggatca cgtggcagca ggatgccctt
     4801 ggcgctcttg ttcagggtgc tgcccggcat ggtgggacag agttccgggt gtttgcgact
     4861 ttgcagtgtg acctcgatgt catcggtgat catcacgtcc gcccgcccct gaatgatctc
     4921 atcgaagatg gtgttgttgt cggggtggcg gatcagggtg gcccgtttca ggttggtttc
     4981 ggcaaactgc tggttggtgc cacccgggtt gacgatcacc tcgatgcccg gttgatcgat
     5041 cctttcaagg cttgagaagc gttcgcgctc agaacagcgg gcgatcggcg tcttgccgcc
     5101 gaggtggtag cgctgcgaaa agagcccgat ctgctgccgc gccagcgtga tgctgacacc
     5161 gctcatcgca aggtcatagc ggtttgccag cagatcgctg ctcaattgtg gccaggaagt
     5221 gcgtacaaat cgcgcctcta ccccccccag gcttttggcc agctcccgtg ccagctcgat
     5281 gtcgatgccg gcaaagccat tgtccgcctg gtgccattcg ctgaacggtg gatagtcgcc
     5341 ggtggtgccg atccgcagat aaccacgcgc aatgatttga tcgagccggc tgtaggccgt
     5401 ggcggcgctg gttgctctga ccgccagcag cggggtgaga agcgcctgtc ggtcggtgtc
     5461 gctcagcagt tcggcatcga cctgccgatt gaactgcgcc tggtgtgccg cgtagagatc
     5521 aacttcgatc aatgtcggca gagcagcctt cagagcgacc aacatccggc tgttgatttg
     5581 atccagtcgt ggccgaatct ccttggccag atcgaccacc tccaatccct gaggaggctg
     5641 ctgctgccac cgctgcaacc aaccgtgctg aagctgtttg cctgcctcca tctgcgcctg
     5701 gaaaaaggcc cgggtggtgg tcgggtcgag gccgagctgc cccgcatcgc tggcaacccg
     5761 atcgatgatc gcctgctcgc gcttgagatc ctcgaccgga agctgatggg cccgcttgta
     5821 gagcgcaacg gctttcatca gttccagccg ctcatcgacc agccgataga gttcacgcag
     5881 cggcggttcc gctgcgtgca gtgacgcgca gcagagcagg ccaaggagtg cggcctgcag
     5941 cagccatctg aacggccggc aggcagttgc cgggagcgcg ccggacaagg cgttcaaccg
     6001 gcgttggcgc tgcgcggggt ggcctgcgcg tcccaatcgg tcaggacgcc gcgcacggtc
     6061 tcgatgaagg ccagcggttc atcgagaaag acgtggtgct gcgcgcgcgg caccgcgatg
     6121 aaaggcacct cgggaccgta gagctgctgc atccaggcag ttgccttgga gttgaagaag
     6181 atgctttcgg cgccatggat caccacccgt ttgcagcgca gccggggaat gtcctcgacc
     6241 agttcgcggt agtccatctt ggtgttgaat tcgtggtcga acttccacat cacgccacct
     6301 tcgaccggca tcagcgaatg acgggcaatg tggtcgatga tgtagtgatt ggcacagggc
     6361 tgttcgggca gcagacggaa gcggctaagt gcggtctcga tgtcgggata ggtctttttc
     6421 ggccggaatg gcgtgctggt ttcgtctcgt ttctcaaagg tctcgggtga catcaccaca
     6481 gtgtcgacga tcatgatgcc tttcaggcga tcgggataga gaaagccggt cttgatggtg
     6541 ccgaagccgc cgaagctgtg ggcgacaatg acggcatcat tgccaaagcc ggccgcttcg
     6601 cagaccgaga tcagttcacg ggcataggtg gcaggggggt acgcctcgcg gtgcccgctg
     6661 tcgcccatgc cgctgatgtc gatggcggcg acagagtggg tgtcacgaaa gaacggggca
     6721 atgaagctcc accagtgggc atgtgcgcca cccccatgga cgaacagcag gcctggcttt
     6781 tgggcccgtt ccgggtcgac ccagtgcagg tagtggatcc ggcacccctc gacctcgatg
     6841 aagtggtctt cgtagggggc ggcaactgca ttgctgaacc aggcgggggg tgaggtgatg
     6901 gttcctgaca tgacggctcc gtgacgggac gaatgactgc ttggacacgc gttttaaacg
     6961 gccgattata caggagcctt agcattttcc gtgccttgcc gcctagcgga cagccgcctc
     7021 ggcagtcggg tcaggccgat ggcatctccc ccgccagcgt ggccagcaga gcttcgattt
     7081 ccagcagccg ttgctgggca tcggggttgg cctgctgcca gctgaatttg agggtggtgg
     7141 caccacgcag ctgatgacgg gccgggtcgc gctgcaccag gcgcaccagg gttagcgggt
     7201 cgatcggggt tttgacatcg aattcgaggc tgccgccact gccaccggct tcgagtcgga
     7261 tgatacccag cgccgtgcag cgcagcttga gcgcgctgat ggcgaacagg ttcctgatct
     7321 gcatcggcag caggccgaag cggtcgatca tctccacctg caattcgcgc agtgccgcat
     7381 cggagcggca actggcgatg cgcttgtaga gcaccagccg gccatgcacg tcgggcaggt
     7441 actcctcggg aatcagtgct ggcaccccgc gcaggttgac atccagcccg ctggccaatg
     7501 gctgttccgg atcatagacc ttgccttcgc gcagggtggc gacggcgcgt tcgagcatct
     7561 ccatgtagag ggtgaagccg atctcgccga tgtgtccgct ctgatcttcg cccagcagct
     7621 cgccggcgcc gcgaatctcc agatcgtggc tggccagcat gaagccggcc cccagggtgt
     7681 cggcctcctg aatcgccgcc agccgtttgg tggcatcggg ggtcagggcc tcgcgatcgg
     7741 gcaccagcag gtaagcgtag gcctgatggt gggaacggcc gacccggcca cgcagttgat
     7801 gcagctgtgc caggccgaac cggtcggccc gctcgatcag gatggtgttg gcattgggaa
     7861 tgtcgatgcc ggtttcgatg atggtgctgc agaccagcac gttgaagcga cggtgctgga
     7921 aatcggccat cgcctgctcc agctcgcgct cgctcatctg tccatgggca atgatgaccc
     7981 tggcctgcgg caccaactcc tgcagctcgc gtgctgcctt gtcgatgctc ttgacttcgt
     8041 tgtgcagata gaagagctgg ccaccacgct gaatctcgcg ggtcaccgcc tcgcggatga
     8101 tgctggccga cttgcggcag acgaaggtct tgaccgacag ccgccgtgcc ggcgcggtgg
     8161 tgatgatcga gatgtcgcgc attcccgaca tggccatgtt cagggtgcgg ggaatcgggg
     8221 tggcggtcat cgtgaggatg tcgacttcgg cgcgcaatgc cttcagccgc tccttgtgcc
     8281 gcacgccgaa gcggtgctcc tcgtcgatga tgatcagccc cagctgtttg aacttcacct
     8341 cgggctgcag caacttgtgc gtgccgatga tgatgtcgac ccggccatcg gccatcccgg
     8401 cgagtgcggc ctgctgttcg cgcgcggtgc gaaagcgtga cagcagctcg atccgtatcg
     8461 gccagtcgac gaagcggtcg cggaaacttt cgaagtgctg ttgggccagc agggtggttg
     8521 gcaccagaat ggcgacctgc cggtggttgg ccagcgcgat gaaggcagcc cgcatggcca
     8581 cctcggtctt gccgaaaccg acatcgccac agatcagccg gtccatcggc ttggcagcca
     8641 gcatgtcggc gatgacggca tcgatcgccc gctgctggtc cggggtctct tcaaacggga
     8701 agtcggcaca gaagcgacga taatcttcgt cgtcgagccg acaggcatgg ccggcacggg
     8761 cggcacggcg ggcatggatg tcgagcagtt cggcggcgac gtcgcgaatc ttctcggccg
     8821 ccttgcgttt ggccttgccc cactgatcac tgccgagccg gtgcagcggc aggtgggcat
     8881 catcggcgcc gctgtagcgc gagatcagtt gcagcgaggc caccggtaca tagagcttgt
     8941 caccgccggc atactccagc atcagaaatt cgctgacctg gttgtcgacg ctcaatgtga
     9001 ccagtccctg atagcgcccc acaccatggt cgatgtgcac caccgccgcg ccggtgctca
     9061 gctcggccag gttcttgaag atccagtcgc tctggtcgct ctcgcggccc ttttttctgc
     9121 gctgtaccaa ctggtggcca aaaagttctg cctcggtcac cagcgcgatg ccctgctcct
     9181 tcagccagag gccgcgctcg atcggcgcca cggtgatggc acagggggct tcgccggtga
     9241 cgaacgcctc ccagccggca ctcggtgccg ggcgcagcgc agcctttttc agcagttcgg
     9301 tcagcgcctc ggcacggccc ggcgattcgg cgacgaagag cagccgactg acgtcgttgt
     9361 gcaggaacgt ctgtaccttg gtcagtgggt gtttggcctt ggcatcgatc gtgaggtcgg
     9421 gcagcgccgg gccatcgtgc tggatctccg cttttccgga gccgagcgcg gtgccgtgca
     9481 gttgcagttg cgggaattgg ttcagccgtt cagtcagcac atcgggtggc agaaacagct
     9541 cggcaggcgg cagcagtggc ttctcgcggt catggcgcag ctcgtcgtaa cggttgccga
     9601 cctgttgcca gaactgctgg gcggcgcgga tcggttcgcc gatggtgacc accaggctca
     9661 aggtcgccag atagtcgaac agggtcgcgc tctcatcgaa gaacagcggt agatagtatt
     9721 ctacgccggg tggcgcgatc ccctggttga tgtcgccgaa gatcggcgac tccttggggg
     9781 tgacatcgaa aaattcatac cagcggcggc gaaaggtggc gatgccggcg ccgtcgagtg
     9841 gaaattcgcg tgccggcagc agctccaccc ggtcgaccct ggcggtggtg cgttggcttt
     9901 cggcatcgaa ggtgcgcagt gattcgatct cgtcatccag cagttcgatg cggtaggggc
     9961 tgctgctgcc catcggatag aggtcgaaga tcgcaccgcg cacggcgaac tcgccatgct
    10021 cgtagacatt ctccaccgcc cggtaaccgg cggcgtcgag ctgctggcgc atccgctcga
    10081 tcgagaaggt ctgcccggtg tgcagttgca gatggtggcc ctgcaggaag tgggtgggtg
    10141 gcagccgctg catcagggtg ggcagcgcga tgaccagcac gcctcgtctg atctgtggca
    10201 actggtagag cagtgacaga cgctgggaga tgatctcctg atgcggcgag aagttgtcgt
    10261 aggggagggt ctcccggtcg ggaaaggtca gcactggcac ctgcggctgg gcggcgagta
    10321 ggaagcgcag ttcggcctcg atgcgcatcg cctcgtcact gtcgatggtg accaccagaa
    10381 tgagccgttc atgcgcggcg gccagctggg cgatcagcag actcggcagc gcgcccggca
    10441 atggggcgat cgccagccgt tcggctgccc gggtgggcag cctgtcgagc agagcagaaa
    10501 aaggtgtcat cttggtcaga gcaggcaggc aggagggagc gggcggatgg gtcacaggcc
    10561 cagtcgccgc caccgtgggg gcgcggcggc aggccctggc tggcggcgat tatccgcctt
    10621 tatcgttcgc gggccaaggc ggacgcgcgt gaagcagtgg ccgggcagca gtgccggttg
    10681 ccctgccatc ctgcgcgggg cgtgtcactc ctcgtcgggg gtgacctcgg ccagaatctg
    10741 ccgaatcttc acctgggccc gctcgctgac cgagactgcg gcgaccacgc cgttgacatc
    10801 gctgcgcagt tgatgctcca tcttcatcgc atcgagcacc accacgatgt cgcccttggt
    10861 aacccgatca cccggtttga cgttgacggc gatcaccgtg ccatcgagcg gcgcagcgat
    10921 cttgccgctg ccggccagat cggcctcttc gggctccttg agcgtgcggt cgatcagctt
    10981 gaggttgccg cgtgcggtgt cgaggtagat ctcccggtcg cggatgaagt agtgcaggcg
    11041 gttgagtaca ccattcagct gatagtggca gcgctcgccg tcgagcgtga tccggtcgat
    11101 ggcgagcgac tcgccgctgg ccgtcgacac cgtgaagctg agctggtttt cgaggttgcc
    11161 ggcgaccttc agcgacactt cgagcgtctc ttcccccagc tcgaagcggt agcggttcag
    11221 ttgccgctgc gcattgcgcc agcctgccag tccggggcgg tgggcgatct tcgccccctg
    11281 ctgcaggtag cggatggccg cagccagtcc cagttcgagg gcgccgggct tgaaggggtg
    11341 caggctcggc tcatcgccca gatgctggtc gatgaaggcg gtggtggctt cgccggcggc
    11401 aaaggccggg tggcgcagga tgctggtcaa aaacgccttg ttgctgttga gaccgagcag
    11461 cacggtgtct tcgacaccgg tgatcagccg gcgacgtgca tcgtcgcggg tctggccata
    11521 ggtgatcacc ttggccacca tcggatcgta gaaggggctg atctcatagc cggtgaccat
    11581 gccgtggtcg atgcgcaggc ccggccgttc gggcgccttc cacatcagca ccgggccggt
    11641 ttgcggcatg aagttgagcg ccggctcctc ggcatagagc cgcacctcga tggcatggcc
    11701 ggtgagcgtg acctgctcct ggctcatcgg cagcttttcg ccggccgcca cccgaatctg
    11761 ccactcgacc agatcggtgc cggtgaccag ttcggtgacc ggatgttcga cctgcagacg
    11821 ggtgttcatc tccagaaagt agaagctgcg gtgcttgtcg accaggaatt cgacggtgcc
    11881 ggcgccgaca tagttgcagg attttgcggc cgcgacggcg gcctcgccca ttttggcgcg
    11941 caactcgggc accatgaagg gcgacgggct ctcctcgacc accttctggt gacggcgctg
    12001 caccgagcag tcccgctcgc ccaggtgaat cacattgccg tgctggtcgg cgaagacctg
    12061 aatctcgatg tgacgcggtt cgatgatcgc ccgctccaga atcagttcgc cgctgccgaa
    12121 ggcgttctcg gcctcggagc gggccgagcg gatctgctcg gccagttggg cctcttcgaa
    12181 caccagccgc atgccacggc cgccgccacc ggccgacgcc ttgaccatca gtggaaagcc
    12241 gacccgcctg gcttcgcgga tcagtgtctc atcgctctga tcggcgcctt catagccggg
    12301 gatggtcggt acgccggcgg cctgcatcgc gatcttggag aggcgcttgc tgcccatcaa
    12361 ttcaaccgcc ggcgccggtg gaccgatgaa gacgatgccg gcagcctcgc aggcggcggc
    12421 gaaggccatg ttctcggaga gaaagccgta gccgggatgc actgcgtcgg cgccggaacg
    12481 tttgcaggca tcgatgatcc gttcgatcga caggtaggat tgcccgaccg gcgaagggcc
    12541 gatcagcacg gcctggtcgg cctcgcggac gtggcgggcg ccggcatcgg cctcgctgta
    12601 gaccgcgacc gtgcgatagc cgagcgattt tgcggtgcgg atgacccggg cggcgatctc
    12661 gccgcggttg gcaatcaaga tttttgaaaa catcagttaa ctccagaccc gtggctgacg
    12721 ggcgccttgc ggcgccgctt tttatcctta atcgtcttgg tgttacatcc ggccaacgcc
    12781 aaaggtgttg ggctgcagct tgcggtggtc ggcctcccaa cacagatcga gcagaaagcc
    12841 gatcagcttg cgggtatcgc gtggatcgat gatgccatcg tcccacagcc gggccgagcc
    12901 gtagatggtg ccggcggcga tgtcgagcgc atccttggtc tctttttcga gcttggcgat
    12961 catctgctcg ctcttctcgt cgaccggcac gcccatgcgg acaaacttgg cttcggagat
    13021 gatgcgcatc accatgccgg cctgggtgcc gcccatcacc gctgtgtggg ccaccggcca
    13081 ggcgaagatg aagcgggggt cgagcccgcg tccgcacatg gcgtagttgc cggcaccgta
    13141 ggaggagccg accacgatgg tgatcttggg cacacgggtg ttggccaccg cctgcagcat
    13201 cttcgagcca tgcttgatga tgccgttctg ctcatactcg gtgccgacgt tgaaaccggt
    13261 ggtgttctgg aagaacagga tcggaatctg tccctggtcg cacagctgga tgaactgcgc
    13321 cgccttggcc gagccggtgt gggtgatcgg gccgttgttg ccgatgaagc cgcagcgcca
    13381 gccttcgatc gagaggtggc cgcagacggt gtgtggatcc cacagtccct tgaactccag
    13441 gaagtctgag ccatcggcga tgcgggcgat gatctcgcgc acgtcatagg ggacgcgggg
    13501 atccttcggc acgatgccga gcagctcttc gttcgggtag agcggctcgc tgtatttcag
    13561 ttgtggccgt ggtggcagtt gctcgttcca ggggatgcgg gcgaacacct cgcgcgccag
    13621 acgcacgccg tcggcgtcat cctcagccag atactcggcg cagccggaga cgctgcaatg
    13681 catctcggca ccacccagct cttcgtcggt ggcgacctca ccggtggccg ccttcagcag
    13741 cggcgggccg gccaggaaca tcttcgcctg ctccttgacc atgatgatgt agtcggagag
    13801 gccaggctgg taggcgccgc cggcggtggc attgccatgc accacggtca cctgcggaat
    13861 gcccttggcc gacatccggc tctggttgca gaaaccgcga ccgccatcga cgaagacgtc
    13921 ggcagcctgg gtcagccgcg caccaccgct ctcggtcagc gagatcagcg gcaggttgtt
    13981 gaccatcacg atctcctgca tccggaagct cttcttgccg gtggccgggc cgccggtgcc
    14041 gcccttgtag gccgagttgg agacgctgat catgcagcgg atgccttcga cataaccgat
    14101 gccgatgatg ttgccgccgc caccgccatc gggatcttcc ggatccatct ggtagccggc
    14161 cagcgtggag agttcgagga agggcgagcc ttcatcgagc agcatctcga tccgctcgcg
    14221 cggcatcaac tggccccgct cgcggaattt cttctcggcc ttgttggcct gctcgatcat
    14281 cttgcgctcg atggcacgaa acgcttcgat ctgctcgagc atgaactcgg cgttctcttt
    14341 gaaggtgctg ctgttggagt cgatcttggt gcgaatgacg ggcatgactg cttccttggt
    14401 acctgcaaaa tctcgtgatg ggttttcgat cgagtggccg tgcggacggg gggattcagc
    14461 aacctttcca gaccggtttg cgcttttcga gaaaggcggt gatgccctcc ttggcatccg
    14521 cggtcttcag gctcttgtcg agcatctcct ttaggaaggg aatggcttca tcgaaggcca
    14581 tcagctcctg gcgatagaag gcttcgaggc caaggcgcat ggcggctggc gagagatcgg
    14641 ccagctcctg ggccagttca gccacccggg catcgagctg cgcggccggc accacctcgg
    14701 tcgccaggcc atgctgctgc gccgttctgg catccatctt gtagccgcgc atgatgaagt
    14761 cgaggccgat tcgccgcggc atcacccgga acagcccggc catcaccatg aacggccaga
    14821 tgccgcgctt gatctccggc gcgctgaaga agatgtggtc ggcggcgacg acatgggtgg
    14881 cgttgcagat gatcagcaga ccgccggcca gcactggccc ctgcgccttg acgatgacgg
    14941 gcttgcagag gtcacgcagc agcgaggcca gctcgcggcc atcgtcgatc ttgggcacgg
    15001 tgctctggct ctgctgcgca gcgccgccgc ccatcgtgcc gaggtcgccg ccggcgcaga
    15061 aggtgtcacc attggcggcc aggaccacca cgcggatgtc gcgctcctgc tccgccaccc
    15121 gcagggcgta ggtgagttct gtgcccaggg tcgggctgat cgaattcttc ttttcgggac
    15181 ggttgagggt gatggtcatg atgtgatcgc tgacgctgac gatcacgcac tcgaacacca
    15241 gcccatcgag tgtgagtgtg gccatggaac tgctccttga ttgttattct gtgccagcgt
    15301 ggatcggcca cgccggcatt tccatctgct tcagcacacc cgcgggattg acagcggaat
    15361 ctcgaccacc ttggcgcgca gatattcgcc cagggtcttg gcctgcggat ccatgcgggt
    15421 cgaggaggag accccatcct gcaggatgtc gtggatgtag aagttcagcg cgaacaggtt
    15481 ggggaactcg tagcgctcga tctcgaacgg acgcacgtcg accatgatct ccttgagccg
    15541 ctccacggtg aggaagtcgc gcaagaaggc gtaggactcg ggcgtcgtgc cccagatgcc
    15601 gacgttggcc gacccgccct tgtcgccgga gcgggctgcg tagatccgtc ccagcgcagt
    15661 gggcgcggtg ggaccggtcg gcaccggcgg aatcggaaag cccggcttgg tgaccactgc
    15721 ctggggcaga ccgagctgct gcgtcggcgc aacgctcttc tgctggccgt cgaaggtcac
    15781 cgtttcactg atgaactcac tgctgaccag ggctggccag tacttgatca gtgcctgccc
    15841 ttcgccggca ctctcgatgg tcgagaaacc aggcacgttg gccaggccca tctcgatcat
    15901 cttcggtgtc agataggggc cgaccagctt ctggttctgc gacaccagat tgatgcggaa
    15961 cagcgagaag gcctcttcgt tggtcggcgg attctctttc tgggtcggga tgaactgggt
    16021 ctcgatcttg tcgaactgct cgcgtccgcc cagcatggtc aggtaggcgt cgaggaaggt
    16081 gttggccttc ttctcggcat ccaggccata gatggcgcag gtcatcttgt ttttgtagcc
    16141 ccccatcgtg ttgatgcaga ccttgtgcga ggggggggga ttcgagcctt tgacgccgga
    16201 gacgaagacc cggtcacggc cacgctgctc gatgcgcagg gtgtcgaagt gggcggtgac
    16261 atcggggttg aggtactggg tgccgcgaat ctcgtacatg atctgcgccg tgaccgtccc
    16321 cacggtgacc gcgccgccgg tgtcggggtg cttggagatg gtgaaggagc catcggggta
    16381 gatctcggcc cagggatagc cgacattgac gaagctggga atctcatgga agaacgagta
    16441 gttgccgccg gtggcctgac agccgcactc gatcacatgg cctgccgcca gcgcaccggc
    16501 caggcggtcc cagtcgtcgc gcttccagcc gaacttccag gccgcggggg ccatcaccac
    16561 cgccgcatcg gtcacccgtg gggcaatgac gatgtcggcc ccctgattga gcgcatcgac
    16621 catgccccag cagccgagat aggcgttggc ggtcaccggt ggaaaggtgc tctcggccag
    16681 cggcatcccc ttgtccatgt tgatgagctg ctcacccttc tgttgcagct cgccgatgcg
    16741 tggcagcagg tcatcgcctt cgatgtaggc caccttcacc ttgagaccga gctggtcggc
    16801 cagggcctgg gtcgcttcgg cgcagcccct ggggttgaga ccgccggcat tggtgaccac
    16861 cttgatgccg cggtcgatgc aggtgcccat cacctgctcc atctgcttca gatgggtgtt
    16921 gacccagccg cgagagccat ccttcaggcg ctgtcggtag aggatcacca tggtcagctc
    16981 ggccaggtaa tcgccggtca gcacatcgat gggaccgccc atcaccagat ccctggcggc
    17041 agaaaccttg tcgccataga agccgctgca gttgccgatg atgattttgt cgttggggga
    17101 acccgccatg gttgctgctc cttaatggcc tgtcgttatt ttctgtctga ggccggccga
    17161 cggctgtttt gtcgccagcc ccgaaatcgc cagcaagggt atacgcagag gcagtcggct
    17221 gacaagactg cccccgattt tgacggggtc aatcgaccgc ttcaccgtgg gcctggcggt
    17281 cggcatggta ggaggagcgc accagcggcg cgctggccac ctggctgaag ccaaggccgc
    17341 gggcgatctc ccccagctcg ctgaattcgt ccggagtgac gaagcggctg accggcaggt
    17401 ggtgccggct gggttgcagg tactggccga gcgtcagcat gtcgacccca tgggcacgca
    17461 ggtcgtgcat cacttcgatc agttcgtcgc gcgcctcgcc cagccccagc atcagccccg
    17521 atttggtcgg aactgccggc tgacgtgcct tgaatgtttg cagcagttgc agtgagccca
    17581 ggtagtcggc accgggacgg accgacgggt agagccgggg gacggtttcg aggttgtggt
    17641 tgaagatgtc gggcggtgcg ctcagcagcg catcgagcgc tggcgcgatg cggccgcgaa
    17701 agtcgggcac cagaatctcg atctcgatgc cggggctccg ttggcgcagc gcggcgatgc
    17761 aggcggcgaa gtggccgccg ccgccatcgc gcaggtcatc gcggtcgacc gaggtgatca
    17821 ccacatagcg cagcttcagt tcggccaccg cggcggccag ttgcgccggt tcgtcgacat
    17881 cgagcggctg gggtcggccg tgggcgacgt cgcagaaggg gcagcggcgg gtgcagatgt
    17941 cgcccatgat catgaaggtg gcggtgccgc cgccgaaaca ctccggcagg ttcgggcagg
    18001 ccgcctcctc gcagacggtg ctgaggcgat gctgccgcag aatcgtgcgg atgcgctgca
    18061 cctcgccaga gctgggcagg cgcacccgga tccaggcggg cttgcgcggc agggtgtcgg
    18121 tggggatcac cttgacaggg atgcgggcga ccttctcttc gccgcgcagt ttgatgccgg
    18181 cggcggcagg gactcccagc ggccgggagc ggatgggcgg ggattcggac atcggctcaa
    18241 ctccagccgg tgagtgggtg gccgtgagta tacgccaacc gctgcatcag atgccggcac
    18301 aacggtggcg tgaccgattc gggcgtggcc tccgccacga agtggctcac ctgggtcatg
    18361 ccgaggccgg gatggccgca ggggttgatc ctccggaacg gtgaaagatc catcgccaca
    18421 ttgagcgcca gaccatgata ggagcagccg cgccgcaccc gcaggccgag cgaggcgatc
    18481 ttctcggcgc cgacatagac gccgggcgcg ccgctgtgcg ttgcggcatc gatgccgagc
    18541 gtcttcaggc agtcgatcac tgcctgctcg atcgcatcca ccagctggcg gatgccgatg
    18601 cccttgcgtt gcagatcgag cagcaggtag atcagcagtt gccccggacc atggtaggtg
    18661 acctgaccgc cacggtcgct ctgcagcacc ggaatggtgc cagggtcgag cagatgctcc
    18721 ggccgaccgg cctgacccag cgtgaacagt ggcggatgct gcacgatcca cagctcatcc
    18781 ggcgtgtggg catcgcgggt cgcggtgaac cgctgcatcg cctgccagca gtcatcatag
    18841 ggttgcaggc cgagggtgcg cagccgcagc aggtcgctga gcccagccgc cgggttcata
    18901 acaccatgcg cacccgcggg tcgcccttga gcgcgccgaa gatcagcgcc agttgcgcct
    18961 cgccctgtgc ctcgatgaag agggtgaccg ccaggtagtt gccgtcacgg ctggggcgga
    19021 tgctgacctc ttcatacagc aaaccggggg catggcggga gacggtggcg acgacgaaat
    19081 cggcgaagtc atcgcgggcc tcgcccatca ccttgatcgg atagcggcag ggaaattcga
    19141 tgcgcggcgg ttcggtgggt tgcatccggg acctcggttg gtgccagggt ggcggggcgg
    19201 tttactgcgc ttcctgtcgc agcgcgtcga agatctgttc cagttgcagc agccgttcga
    19261 gtggatcgtc gagcgtcagc agcgcctgct tctcatcggc cggcagcggc agcagttgcg
    19321 acagttgcca gcccagcgtg cgcgcctcgg tggggtcgct cgccatcgcc agccgttcga
    19381 cgaaaggata ctccagcagc tgctgcaggg tgctctgcag gatctcgaag cgaccggccg
    19441 gaacctccag cggcggttct ggcgccaacc actctacctc ggcctgcagc cagcgactgg
    19501 gggtgctggc gtggtgcgcg atgctgaact tgcgtccacc ttcgaccagc agtccgagcc
    19561 ggttgtgccg cagctgctgg aagtcgacga tgcgcgcttc agtgccgatc gggtggaacc
    19621 gcggcagctc gcccacctcc tcgccctcga tgatggtgac gacaccgaag tcgaccttct
    19681 gtttcatgca gaaggagacc atctccagat agcgcggctc gaagatctgc aactggatcc
    19741 ggccactggg aaagagcgtg gtcttgagcg gaaacagcgg gatcgaactg ctcatcgccg
    19801 actcagggct gccgctcccg ggccacggcg cggtagccga tgtcgcggcg acagtaggcg
    19861 ccccgccact ggatttgcgc cacgttgcgg taggccagtg cctgtgcggc ggcgacgctg
    19921 tcgccgagcg cggtgacgca gagcacccgg ccgccggcgg tgaccacctg gccgtcatcg
    19981 aggcgggtgc cggcatggaa caccttgcag tcgggcagtg gctgtgcgag tccctcgatc
    20041 ggatcgccgg tggcatactc acccggatag ccgccagcgg ccatcaccac gccgagtgcc
    20101 gggcgcggat cccagcggca ttcgcagcca tcgagccgct catcgagcgc tgcgctgatc
    20161 agctcgaaca gatcggactg caaccgcagc aggatcggct gcgtctccgg atcgccaaac
    20221 cggcagttga attcgagcac tctgggttcg ccggccgcat cgatcatcag gccggcatag
    20281 agaaagccgt ggtaggggtg cccctcggcg gccatgccgg ccagcgtcgg ctgaatgacc
    20341 tgggccatga tgcgctggtg cagcgccggc gtgatcaccg gtgccggtga gtaggcgccc
    20401 atgccgccgg tgttggggcc gaggtcaccg tcgtcgcgcg ccttgtggtc ctgcgaggtg
    20461 gccagcgcaa tcacatgccg gccatcggcc atgacgatga agctggcctc ttcgccggtc
    20521 agaaactcct cgatcaccac ccgatggccg gcatcgccga aggcgttacc ggccagcatc
    20581 tcctgcaccg ccgccttggc ctcgtcgacg ctctgtgcca cgatcacccc cttgccggcc
    20641 gccagcccat cggccttgac gacgatcggg gcgccgcagg catcgatgta ggccagtgcc
    20701 ggcgccacct cggtgaagct ctggtaggcg gcggtcggaa tgtggtggcg tgcgagaaag
    20761 tccttggcga aacgtttgga gccttcgagc tgcgccatcg cccgggtcgg accgaagcag
    20821 gccagcccct cggcctggaa gcggtcgacg atgcctgcca ccagcggcac ctccggcccc
    20881 accaccgtgc agcgcacctg ctgctggcgc gccagttcga ccaggcggtc gaactcgagc
    20941 ggatcgatgg cgacattctc gaccttgggc tcactggctg tgccggcatt gccgggcgcc
    21001 accagcaccc tggccacctg gggcgactgt gccagcttcc aggccagtgc atgttcacgg
    21061 ccaccggagc cgatcaccag aacagtgtgg gcagacatcg aatggactcc tgatcagtgg
    21121 cggaaatggc gcatgccggt gaacagcatg gccatgtcgg cttcatcggc ggcggcaatc
    21181 acctcggcat cgcgcagtga gccgccgggc tggatcaccg cgcggatgcc ggccgctgct
    21241 gcggtgtcga tgccatcgcg gaacgggaag aaggcgtcgg aggccatcac cgaaccggcc
    21301 accgtcagcc cctcgtcggc ggccttgatg ccggcgatgc gggcgctgta gagccggctc
    21361 atctgtccgg cgccgatgcc gatggtgctg ccgccacggg cgtagatgat cgcattggat
    21421 ttgacgaact tcgccaccct ccaggcaaac agcagatctt ccagctcctc ggcagtgggt
    21481 gcgcggcgtg tcacctgtcg caactgggcc gcgctgacct gtcccagatc gcgctcctgc
    21541 accagcaggc cgccctgcac ccgcttgtag tcgagcgccg gctgcggttc accccaggcg
    21601 ccggtgacca gcagtcggac attgggtttg ctggccagca ccggcagcgc ctcctcgctc
    21661 accgccggcg cgatgatcac ctcgacgaac tgccgttcga tgatggcgct ggcgcaggcc
    21721 agatcgagcg gacggttgaa ggcgatgatg ccgccaaagg ccgaggtcgg gtcggtccga
    21781 taggccagct cataggcggc gagcggggtg gcggccaccg ccacgccgca ggggttggca
    21841 tgcttgacga tcacgcaggc cggttcgctg aagctcttga cacattcgag cgcggcatcg
    21901 gtgtcggcca cattgttgta ggagagcgcc ttgccctgca gttgccgtgc agtggcgatg
    21961 cccgcctcgc ggctgtcgcg ctcacggtag aaggcggcgc gctgatgcgg gttttcgccg
    22021 tagcgcatcg gttcgacctt gtcgaactgc aggttgaggc tgcgcggaaa gcattcgccg
    22081 tcgacttcgt cgaggcggcc cagatagttg gcaatcgcat cgtcatagcg ggcagtgtgt
    22141 gcaaaggcga ccccggccag ccgcagccgg gtggcggcgc tcaggctgcc ggtcgacgcc
    22201 agttcgctca gcagccgttc atagtccgcc ggctcgacca ccacggcgac atcgcgatgg
    22261 ttcttggctg ccgcccggat catcgtcggg ccgccgatgt cgatctgctc gatcgccatc
    22321 gccaggtcgc agtcggggcg ggcgatggtc tcggcgaacg gatagaggtt gaccaccacc
    22381 agatcgatgt agtcgatgcc atgggccgcg gcgacggcgt catcctcgcc acggcgcccg
    22441 agaatggcgc catgcacctt cggatgcagc gtctttaccc gtccacccat gatctccgga
    22501 aagccggtgt agcccgacac ctcctgcacc gcgatgccgg cctgctgcag ggcacggtgg
    22561 gtgccgccgg tcgacagcag ctcgatgccg cgggcggcca gcgcctgggc gaagggaatc
    22621 aggccggtct tgtccgacac gctcaacagc gcgcgccggg gggtcatcga atcgggtgag
    22681 gaggggctgt tcatctctgc tctgaaagac tcaagggtga ggcggggtcg agggcgcgcc
    22741 atccgggtgg tgggtgaacc ggcaccggca cggtcgaaag gccggcgatt atacgaaaaa
    22801 gccgcccgtt tcgcgcgtga agcggggagg gggagagcgc gcggccacgg cccgcggcgc
    22861 cggcgatgcc ggatgaacgg ccgtgactac ttcaggttga tcacatgcag gccgcactcc
    22921 ttgtgggcgg cctcttccca ccaccagcgc ccctcgcgct cgtgctggcc gggcagcacg
    22981 ggccgggtgc agggctcgca gccgatgctg acgaaaccgc gctcgtgcag cgggttgtag
    23041 ggcagatcga agctgcggat gtagtcccac acctgctgcg aactccagtt ggccagtggg
    23101 ttgaacttga ccagctgccg cgcttcgctc gaaaaggccg ggtcgagctc catcaccgga
    23161 atctgctggc gggtcgaggg gctctggtcg cgccgttgac cggtgatcca gccatcgagg
    23221 gtggccagtt tcgcccgcaa cgggttggtc ttgcggatct ggcagcactc ctggtggccg
    23281 tcacggtaga agctgaacag ccccttggcg ttgaccagct gccgcacctg gtcggcgtcg
    23341 gggaagagca gctcgatcgg aatctgatag tgctcgcgca cccggtcgat gaagcggtag
    23401 gtctcggggt gcagtcgacc ggtgtcgatc gagaagacct gcaccgcctt gttcagcttc
    23461 acggccatgt cgatcagcac cacatcctcg gcaccgctga aggagatggc gatggcgtcg
    23521 aaccgggcaa aggcgtggcg caggatctcc cgcgggcttt tggcggccaa ctgttcggcc
    23581 tggctggcaa tgtcggcggg ggagagctgc atggcaatgg cccgtggctt gatctgaagt
    23641 ggtgtgctgg atcgggcgcg cagtgtaaca acggtgtgac agcggcataa agatcaaatc
    23701 gttttatcga tagaagccgg attcatggcc agtcaggcca tgaacggtgg gggcgccatc
    23761 gagcagcgac ccgatcgggg cgggttgata tactgacgcc accaagccgg agccggtccg
    23821 gcgctgtttc accatgcaaa caaggggtaa gccgtggaga tcgcctgtct tgatctggaa
    23881 ggggtgctgg ttccggagat ctggatcaac tttgccgacg aaaccggcat cgccgagctg
    23941 cgcgccacca cccgcgacat ccccgactac gaccggttga tgcgccagcg gctggcgctg
    24001 ctcgatcagc atggcttcgg cctgcccgac atccagcggg tgatcgaccg catggggccg
    24061 ctgccgggcg cgctggagtt cgtcgactgg ctgcgcgagc ggttccaggt ggtgattctc
    24121 tccgacacct tctatgagtt cgcccagccg ctgatgcgcc agctcggctg gccggcgctg
    24181 ctctgtcacc gcctgcaggt caacgagcag gggcgggtgg tcgactacct gttgcggcag
    24241 aaagatccca agcgtcacgc cgtcgaggcc ttcaagagcc tcaacttccg ggtgatcagc
    24301 gccggtgact cctacaacga caccacgatg ctggccgcct cggatgccgg catcctgttt
    24361 cgtgcgccgg ccaatgtggt ggccgaattc ccgcagttcc ccacggcccg ggagtatgac
    24421 gagctgaagg agcgttttct ggccgccagc gacaggaaat tgtcgcgctg aacggctgcc
    24481 tcgacacatt cagataaaga gcgcgttcca cgcgagcaga cgccagcgac tgaccagacc
    24541 ttcggtcacg aatgggtcgc cctgaatgaa ctcctcggcc ccctcgcggc tggcgaagat
    24601 gcccatggcc ccctccgtcg ggtcaccgaa gggcccggcc gccagcacga tgccgcgcgc
    24661 atggaacgca tcgagcctgg cacgatgcgc cgggtagtgc gccctgattt tcggcaaggc
    24721 atcgggcgcc atttcataaa aagagaccac tttcatggca gttttccatt tggggaacag
    24781 gggctggcaa gttcaaggca aaaggcgggc ggtggcgctg accggcaagc gcatcgattc
    24841 gcagcgacga accggatgtt cggcccgcgc cctcgtgcgg cggcgtggcc gatctgagct
    24901 atcatggcgc gaaactggtc aaggagtctt tcatggtcaa gccgatccgc ttcaccgacc
    24961 gctttgcggt ccatccgcag attcgtcccg aggcgctcga agaggtggcg gcgctcggct
    25021 accgccgggt gatcaacaac cggcccgatg gcgaggagcc gggacagcca cgcagcgccg
    25081 aactggaagc gaaggcgaag gcgctggggc tggagtacca ccacctgccg atcacgctgc
    25141 cgggcctgca tgaggcgcag atcgagcaga tggaggcact ggtggccgat caggcaccgg
    25201 tcttcgcctt ctgccgcacc ggcacccgat gcgcgatcct gcatgccatc atggcggtgc
    25261 gtgccgggcg gccgctggcc gagctgctgg ccgaggcggc tcgcaatggc tatgacctca
    25321 ccgcccagga gccgctgatt cgccacttcg gcgatcagtg agcgcgcagc ggatccggcc
    25381 gtgccggccg ggtctcactc gtcccagggt ggcagcaggc cgtggtcgac cggggcgaag
    25441 ctgtggtgcg gcagtgactc ggcgccatcg accttcaggg tttcaccgct gatgaagttg
    25501 gcggccggcg agagcaggaa gcagatggcg gcggccacct cggcctcgct gccgaggcgg
    25561 taggtctggt tgtgcttgcc cgcctgcagg aagaacttct ggaactccgg gtcgtaggtc
    25621 ttgatgccgc tggagtagat cgcccccggc gccaccgagt tgatgcgcac gccgtagcgg
    25681 ccccattcga cggccagggt cttggtgagg ttgtcgacgc cggcacgtgc cgcgccggtg
    25741 tggcacatgt tgggaaaacc gttccacatg ttggcgatga tgttgacgat ggcgccgccc
    25801 ttctccttga aggcctggtt gaaggcctcc tgggtgacga agaaggtgcc attgagattg
    25861 gtgtcgacga cggcgcgcca ccccttgggc gagatcttct cggccggtga cggaaactgc
    25921 ccgccggcgt tgttgaccag aaagtcgagg gtgccgcagg tggcgacgac ctgctcgaag
    25981 aagctcttga cctgttccgg ctcgcggatg ttgagcggga atgcgtgcac cgtgccgcct
    26041 tcggctcgaa tcgtctcgac gctgcttttg agcttctctt cgttgcgtcc gccgatcacc
    26101 acggtggcgc ccagttgcgc cagttcgcgc gcggtgcgca ggccgatgcc ggagccacca
    26161 ccggtgacca ccgccacctt gccggcaaag aggttggcac tgaatacgct tttgatcatc
    26221 gtgagttgct ccttgagtcc ctgttgttat cgtctggctg tctccagcgt gatggcgcat
    26281 tctccccgct gccggtcggc gaaatcaata gcgacgccgt gagcggggcc ggacgtgacc
    26341 gcgacacgcc agtggcagcg gcttcggcta taatgccgcc ccctttcacc gcccgcccat
    26401 tttcaggtgc cttgccgtga cccagcagat tcgcaacatc gccatcatcg cccacgtcga
    26461 ccatggcaag accacgctgg tcgatcggct gttgcagcag tcgggcacgc tgaagcgggg
    26521 tgaggagcag atcgagcgtg cgctcgactc caatgagctg gagaaggagc gcggcatcac
    26581 cattctggcc aagaacaccg ccatcgcctg gcagggcaac cgcatcaaca tcgtcgacac
    26641 gccgggccac gccgactttg gcggcgaggt ggagcgggtg ctgtcgatgg tcgactcggt
    26701 gctgctgctg gtcgacgcgg tcgatggtcc gatgccgcag acccgcttcg tgacgcagaa
    26761 ggcctttgcc cagaagctca atccgatcgt ggtggtcaac aaggtggacc gtcccggcgc
    26821 gcggccgcac tgggcggtcg atcaggtgtt cgacctcttc atcaatctgg gtgccaccga
    26881 agatcagctc gactttccgg tggtctactg ctcggcgctc aatggcatcg ccgggctgga
    26941 gcccgatgcg ctggccgagg acatggcgcc gctgttcgag accatcatgg cgcgggtgcc
    27001 ggcgccgcag gtcgacctcg acggcccgtt gcagatgcgc atctcgaccc tcgactactc
    27061 cagctatctc ggcatcatcg gcatcggccg catcaaccgc ggcagggcgc ggcgcggcca
    27121 gccggtgacg gtggtggccg ccgatggcag ccgccgtgcc ggcaagctgg cgaccatcat
    27181 gggctacatg gggctgggcc gggtcgaggt cgaagaggcc gaggccggtg acatcgtctg
    27241 cgtcaccggt ttcgatgagc tgaacatctc cgacacgctc tgcgacccgg cgctgcccga
    27301 ggcgctgccg gcactcagtg tcgaggagcc gacggtgtcg atgacctttc aggtcaacac
    27361 ttcgcccttc gccgggcgcg aaggcaagta tgtcacctcg cgcaagatca aggagcggct
    27421 ggaggaggag ctcaagcaca atgtcgcgtt gcgggtcgag gcgaccgaca gcgccgaccg
    27481 ctttcgcgtc tccggccgcg gcgaactgca tctgggcatc ctgatcgaga ccatgcgccg
    27541 cgagggctac gagctgggcg tctccaagcc cgaggtgatc atcaaggtcg aaaatggcga
    27601 gcggttcgag cctttcgagg agctggtggt cgacatcgag gagacccatc agggggcgat
    27661 catggagacg ctgggggcgc ggcgtggcga gctgcaggag atggtgcccg atggcaaggg
    27721 gcgggtgcgg ctgcgctatc gcatcccggc gcgggcgctg atcggttttc gctccgactt
    27781 catgaccatg acctccggca gcggcatcat gagccacaac tttctcgcct acggcaaggc
    27841 ccatccgggt gacgtgggtg ggcgcaacaa cggcgtgctg atcgccaatg gccagggcaa
    27901 ggcgctcggt tttgcgctgt tcaacctgca gaaccgcggc cgcctcttca tcggtccggg
    27961 tgaggaggtg tatgagggga tggtcatcgg catccattcg cgcgacaacg acctggtggt
    28021 caacccgctg aagggcaagc agctcaccaa catccgcgcc gccggctccg acgagaacat
    28081 cgtgctgacg ccaccgatcc ggatgacgct ggagcgggcg ctggagttca tcgccgagga
    28141 tgagctggtc gaggtgacgc ccggcagcat tcgcctgcgc aagaagcaac tgaaagagag
    28201 cgaccggcgg cgcgattcgc ggtcggcctc cagcgaatga gactcaggcc gtcactgtga
    28261 cagcagtgcc cggtagccgt cgcggtagct ggggtagcgc aactcgaagc cgctgcgccg
    28321 cagccgctgg ttgctgcagc gtttgctgcc gcccaggcgc tggctttcgc cgcgccgttg
    28381 cagggtgacg ccgagcagcc cggcgatgaa tgcctccacc tcgtgcagcg gtgccggttc
    28441 atcgtcgaca ccgaggtagc actgcgccac tggcagcccc tgccggtcac gttcgaccag
    28501 aaagtcgagg aggcgcgccg catccgcact gtggatgcgg ttgccataca gggttggcga
    28561 gagcgggctg ccctggccgc tgcggacccg ttcgatcaga tggttgcgtc ccggtccgta
    28621 gatgccgccg aggcgcacca gcgtggtcgg caggccactg gcgagcgcca gccgttcggc
    28681 ttcgagcatg atgcgacccg agaagcgctg cggctcggtc gggctctctt catcgaccca
    28741 ctccccctgt gactggccgt agacgccggt gctcgaggtg aacagcaggc gccggggcgc
    28801 ggccggcagg gcggcgagca ggttgcgcag cccctcgaca tagagcgcgc ggtaaccggc
    28861 ttcgctggaa tgctcggcgg ccgcggtgaa cagcaggtag tcgacggcgg gcagcgcgcg
    28921 cagcgtggca ggatcgccca gatccgcgct gcagggacgc accccggtcg gcagcaggct
    28981 gctctggcgg cgcaatcccc acacctgtgc accgcggacg gcgagcagcg tcgcggcctg
    29041 actgccgaca tcaccacagc cggccaccag cactcgcaac ggttcgctca tctcgacacc
    29101 tgcttcgata aatggggctg ccaccatacc atagcgccca tcggcagttc agagacagtg
    29161 atgcagagat gacccaggcc gtcgatttct ggcaggcacc cctcacccgg ctcagtggcg
    29221 tggggccgca gttggcggcc cggctggcgc gtcttgatct gcaccggatt gaggatctgc
    29281 tgttccacct gccgcaccgt tatcaggacc gcacccgcct gacgccgatc ggccagcttc
    29341 gccccggtca tgaagcgctg gtcgagggtg aggtgcagtt cagcgagatc gcacccgggc
    29401 gccggcggac attgctgtgc cggatcagcg atggccgtgc cgccctgacg ttgcgtttct
    29461 ttcacttttc gccgaagcag cagcagtggc tgcgcgccgg tctctggctg cgctgccatg
    29521 gcgaggtgcg cagcggcatg gctgggctgg agctggtcca tcccgaactc gaactgctga
    29581 gcgcgccggg ccagccgccg ctgtcccccc atctgacgcc gatctaccct gccaccgacg
    29641 gcttgacgca gacgcggctg cgcaacctga tcgatcaact gctgctgcgg atgcagcaac
    29701 tgggcgcgcc acctgactac ctcgctggcc tcactgcaca acagggggat gactggagtg
    29761 aactgctgcg ggccgtgcac catccgccgc cctcgcagga tgcggcgctg cgtcagcagc
    29821 agctcgaccg gctgcgccgc catctggccc gcgaggagct ggtcgcccat cagctgacat
    29881 tgggacggct gaagcagcag gcgcagcggc accgtgcacc acccttgccg gtggagcaga
    29941 gccgggcggc ggccgaacgg ctggcggccg cgctgccgtt tcggctcacc gcggcgcagc
    30001 agcgggtgat tgccgagatc ggcgccgatc tgcaacagcg tcggccgatg cagcggctgg
    30061 tgcagggtga cgtcggctcc ggcaagacac tggtggcggc ctttgcggcg ttgcaggcgc
    30121 tggagagtgg ccaccaagtg gcgctgatgg cgccgaccga gctgctggca cggcaacatg
    30181 ccgagaactt cgaccgctgg ctggcaccgc tgggttggga ggtggcgctg ctcagtggca
    30241 gccagaaggg caaggtgcgg gccgaccagg cgcaggccgt cgcctctggt cgggcgatgc
    30301 tggcggtcgg cacccatgcg ctgtttcaga catcggtgca gttccagcgg ctggggctgg
    30361 tgatcatcga cgagcagcac cgcttcgggg tacatcagcg gctggcgctg cgcaacaagg
    30421 gcggcgagca gctctggccg catcagttga tcatgaccgc caccccgatt ccgcgcacgc
    30481 tggcgatgag tgcctatgcc gacctcgacc tgtcgctgat cgatcagttg ccgccgggtc
    30541 gtcagccgat caccacttcg gtcatcaatg cggcgcgccg cgaccaagtg atcgagcgga
    30601 ttcgcctcaa ctgcgcccag ggacggcagg tctactgggt ctgcacgctg atcgatgcct
    30661 ccgagcagat cgaggcgcag gcggcggccg acaccgccgc gcaactggcc gccgcgctgc
    30721 cgcaggtgcg ggtggccctg ctgcatgggc ggatgaagga ggcggagaag cagcagacga
    30781 tggcccgctt caaggcggcc gagatcgatc tgctggtggc caccaccgtc atcgaggtgg
    30841 gggtggatgt gcccaacgcc agcctgatga tcatcgagaa tgccgaacgg ctggggctgt
    30901 cgcagctgca tcagctgcgc ggccgggtcg gccgtggcca gatcgacagc cactgtctgc
    30961 tgctctaccg cgcgccgctg tcgccgaatg gccgggcgcg gttggcggtg atgcgtgaat
    31021 cgcaggatgg ctttcgcatc gccgaagagg atctgcggct gcgcggcccc ggcgagctgc
    31081 tgggcacccg ccagaccgga ttgatgcagt tcggccaggc cgacctcggc cgcgatgccg
    31141 acctgctgcc ggaggtgaaa cagtgggccg agcggttgcc ggcacaccgt cccgaactga
    31201 tcgatccgct gatcgcgcgc tggttcgcgc acaaggaggg attcatcacg gtatgagccg
    31261 cccggcacgg tgcgttgatc aatctctggt ctacacttca ggggaagttt cgattcatgc
    31321 ctgcggccga tgtacggccg tgatgactgc ctggagcgtg ccatgtcttt tgccaccgcc
    31381 gttgcgtccg atccattgat gacgccggat caacccatcg aggccgcgat gcgactgcgt
    31441 ccgctgctgc tggagcgggg tggcaagcag aagttgatgc tgctgaccgg tgccgaccgc
    31501 atggtgcagc tcgaaccgct gtcgcggctg ctcggtgccg atctgcgtgc cgctcgcccc
    31561 ggcgatcagg cgcattggag cgcgaagtgg ctggcacagg gcgcggacga ccgcctgccc
    31621 tgcatcatcg atgcagccct gatcgatgcg ccacgcggct tcatcgagat cgatggcgat
    31681 ggccagagca ttcctctttc cgaagagagc tggcgcggat ggtgcgatgg cgctcgcatc
    31741 gccgaggttt cgctgccggt cgatgaactg gaagcggcct atctgcacgc ggaacaggca
    31801 cgcatcgagg ccgagcgcga ggaggatggc ggtgagagct tctctgcaca gcgcatccgt
    31861 gcccggctga gcgagacgct gtcgattccg ccgctctccg aggtggcgcg ccggttgatc
    31921 cagttgcagg ccaatcccga cgccaccggt gaggatctgg cgcgcatcgt cgagtgcgat
    31981 gccagcctgg ccgcgcaggt ggtggggtgg gcgtcatcgc ccttctatgg caagcgtggc
    32041 ggcgtgcgca cggtgcgcga ggcgatcatc cgggtgctcg gcttcgacct ggtgctgaac
    32101 ctcgccatcg gtctggcgat gaagcgcagt ctggggcagc cgcaggatgc accgcagggg
    32161 ttgacctcct acgcccggca gtcgatctat ctcgcggcga gcacggcgct gctgtcgacg
    32221 atgatgccga ggaaccagcg gcccacgtat ggcgcggctt atctctgcgg cctgctgcac
    32281 aactatggcc acctgctgct gggttgtctg tttccgccgc acttttcgct gatctgtcgt
    32341 catcaggagc tcaatccgcg gcttccacgg cactggatgg agcagcagct gctgggcgtg
    32401 acccgcgaac agatcagttg ctggatgttt cggcagtggt cgctgcccga tgaggtggtt
    32461 tccgccattc agagccagca tggtggctgg ggcgagtccc gccatgcctg tcttggccag
    32521 ttgctctatc tgtgcagtcg cctgctgcgc cagcgtggcg tgggcgacct gccgtgcgat
    32581 ctgatcgacc cgcaactcta cgtggcgttg cagatcgatg aggccgaggt ggccttgcgg
    32641 ctggatgagc tgatcgagaa ttcagaaacc ctcgaccagt tggcacagag tctggggcgt
    32701 tgagcggcag gcttgcggag gagcggtcta cccgtttccg gccatccacg ccttcaactg
    32761 tttgcccggg cggaacagca cggtggtgtg tgccgggatc tccagcgcgg cgccggtgcg
    32821 tggattgcgt ccagtgcggg cgttgcgccg gcgcggagtg aagctgccga agccggtcag
    32881 cgtcaccgat tcaccccgct gcaatgcccg ggtgatctga tcgagaatcg catcgatcac
    32941 gcaggccgtg cgctcgggac tcatgtcgat gcgctcggcc acctcgcgga tcagttcgct
    33001 cttcgtcacc gggtgcgtgg cctgacagtt tccgaaggtc gggcgggcag caacgaattc
    33061 agaactcgac catctcgaac atctccttgc ccaccgcgca gagtgggcag atgaaatcct
    33121 cgggcacatc ctcccagcgg gtgccgggtt cgatgccctc ttcggggcac cccaaggctt
    33181 cgtcatacac ccatccgcag acgatgcact catacttctt ccagtcactc attggcggag
    33241 tcccatgcag gtggttcggt ggctctgctg ataacggttg gcgctgcgcg gcaatggcaa
    33301 ggcgccagag cggccgacat tctagccgat ccccggcggt ggtgcagtcg ttgcttcgcc
    33361 tgatgcaggc agtggaatgt gcggccggcg caacagaaag gcgtgaccca aaccgcggtg
    33421 gcggtagaat cgcccgattg tggtctggat gctcttcatg ttcccgcgcc gtcgacctct
    33481 gcacgccttc attcagttga cccgactcga tcgtccgatc ggcatctatc tgctgctgtg
    33541 gccggtcgcc tgggcgttgt ggctggcggc ggatggctgg ccgggctggc gccacctgct
    33601 ggtcttcacg ctggggacgg tggtgatgcg ttcgctgggc tgcatcatca acgactgggc
    33661 tgaccgcgac cttgacggcc atgtggcccg cacccggcag cgaccgctgg tcaccggcct
    33721 cgccagcagc cgcgatgcgc tcggctatgg cctgggcctg ctgctgctgg cgctgctgct
    33781 ggtgctgctg accaaccggc tgacgatgct gctcgccatt ggcgcgctgc tgctggccgc
    33841 gctctacccc ttcatgaagc gccacaccca cctgccgcaa ctggtgctgg gggcggcctt
    33901 tgcctggggg gtgccgatgg tctttgccgc ggtgcaggag cgggtgccgg ccacggcctg
    33961 gctgatctat ctgaccaccc tgctctggac cgtcgcttac gacaccctct atgcaatggt
    34021 tgaccgggag gatgacctga agatcggcat caaatcgacc gcgatcctgt tcggcagtgc
    34081 cgaccgggcg atggtcgccc tgctgcaact gctgagtctg ctcggctggg cgctgatcgg
    34141 tgtcaacttc gatctgggcc tctgctatgc gctggcggtg gtggcggccg ccctgctgtt
    34201 cggtcatcag cagtggttga tccgcgatcg ggcacccgcc gcctgtctgg ccgcttttct
    34261 gcacaatcag tgggtcggtg ccgtgctgtt cgtcggcatc gtgctcgatc aactgctgcg
    34321 ctgacggagg tcatgtggaa gttcgcctca gccaccggat cgacgagatc gatccagcgc
    34381 ggtgggatgc agtggtcggc cgcgactatc cctttttgcg ccacccgttt ctgcaggcac
    34441 tggaggccag tggcgcggtc agtgtcgaga ccggctggca gccgctgcac ctgacactgt
    34501 gggagcagga gcgtctgctc gggctgatgc cgctctatct caagagccac tcctatggcg
    34561 agtatgtctt cgactggagc tgggccgatg cctaccgccg ccatggtctc gactactatc
    34621 ccaaactggt gacggcgatc ccgttcacac cggccagcgg accgcggttg cggctggccg
    34681 acggcctcga cagcgccgag gcgatcggac gggtgtcgat gaccctgcac aacctggccg
    34741 aacgtctggg cgcctcgtca tggcatgggc tcttcttcga accggcacaa ctgccgcact
    34801 ggctcgatca gggccatctg ctgcgcaagg gctgccagtt tcactggcac aacccggggt
    34861 ttcgcgactt cgccgatttt ctcgacggct tcaactcgcg caagcgcaag gcggtgcggc
    34921 gcgagcggcg tctggtcgaa gagcaggggc tgcgcctgga gcggctgacc ggcgaccagg
    34981 tcagcgaggc gctctggcag cgcttttacg cctgctacca gtcgacctac cggcagcgtg
    35041 gccagcgcgg ctacctcaac ctggacttct ttctgcgtct ggcgcaacag atgggcgaat
    35101 cactgctgct ggtggtggcg cgccgcgagg cagaggtggt ggcggccgcg ctcagtctgc
    35161 atgacgacga gacgctctac ggacgccact ggggcgcgct cgagcagcat gatcaactgc
    35221 acttcgaggc ctgctactac cagggcatcg agttctgcat cgagcgcggg ttgaagcgat
    35281 tcgacccggg cacccagggc gaacacaaga ttcagcgcgg ctttgccccg gtcgaaaccc
    35341 attcgctgca ctggatcgcc gatggccgtt ttcgccgggc gatcgccgac ttcgtcgccg
    35401 aagaggcgct cggcatcgac cgctaccgcc agcaggccgc gacgctgctg ccgttccggc
    35461 agggtgactg agcgtcgtcg cagagctcag tccgctgtcg gcggcaacgg tggccgcatc
    35521 gtgatggcca tcaccaccat cagcagcgcg atggcggcaa agccgagaaa gaccggccga
    35581 tagctgtgca ggctgtcatg cgccatggcc gcagccaccg gtccgaccgc ccccagcgcc
    35641 gtggccacca ggctgcgcac cgaaaacagc cgggcatagc tgctggcgcc aaagtagttg
    35701 gcgatcagcg gaaagctcgc caccgaggcc atcccatagc cgatgccgaa cagcactgcg
    35761 aacaggtagg cgatccaggg ctgggtggtc tggtcgagca ccacgatgcc aagcagttgc
    35821 accagcaggc cggcaccgag cagatagcgg gggtcgtagc ggtcgcccag caccccggtg
    35881 agcagccgtc cgccggtgtt gaacagcccg atcaggccga gcacggtggc gctcagcacg
    35941 atcgaaagcc cctgatcctg cagatgcagc atcgcctggc tgttgacgat ggtgttgccg
    36001 atcaccacaa aggcgccgct gatcacgatg gcccagaatg ggtaggtgcg cagcgcctcg
    36061 cgaaaggtcc agggatattg ggtctggtgg acatggctgc gcttcaccgt ggtcgctgcc
    36121 gcattggcct gggtggcctt gcggccatcg ggcacttcgc cgacctcctc gggtgattcc
    36181 ttcaccgaaa ccagtgccag cagcgaggca gccagtgtgc agagcgccat ggcgacgaac
    36241 accagccgcc agttgtggct ggcggcgatg atccaggcaa agctgggcgc cgcgacgaag
    36301 ccgcccagac ccgacgccgc catgaagacg cccatcgcca gcgcccgacg ccggctgaac
    36361 cagcgggtca gcaactgggt gcccggcagc acggtctgta tcgtcatgcc aaggcccagc
    36421 acggcggtgc cgaggtagaa cagcggcagc gaactgcaca ggtagaccac ggccgcaccc
    36481 acggccgcca ctgctccgcc gagggcgatg cagaagcggc tgccgcggcg acggatcagc
    36541 cagtccgcca caaagggggc gcaacccagc atcaggttga tgatcgaaaa gcccgcgctc
    36601 acctcggtgc ggctccagcc catgctggca accacttcgg ggatgaccac cgaaaagccg
    36661 taatagatcg atccgagcga aagaaaatag atcagcgaca gtgccgccag cagtatccag
    36721 ccgtaaaaca tgagtgttcc tgttgccgct gccgatgggc agggtggggc cgccggggac
    36781 cccggctccg catcggtcta tcgaatccgc acagtggcga agagttcgtc agccctgtcg
    36841 acctgggtgt ggaaacctgg cctctttggc atggcgcctg gtcggggaga aaccggttct
    36901 ccccttgggg tcgagtctca gtgccagcag ccggcggcgc agcggtggcc ggtggctgct
    36961 ggcaccgcag ccgctggttc ggccttggca ctgctgcgac cgctcatcac ctgaccgccg
    37021 gtggccagtc ggcgcaccgg tgcatcgagc ggagtgcctt gtgcatcgat ggcggccagt
    37081 tcgcacagct caccccatga gtgcacttcg ctcgacatgg cgtgtttgac ctcgacgact
    37141 tggccgttgc tgtcgcagat gtaatcgtag gtgggcatcc cgatctctcc aggctgtggg
    37201 tggcggtcaa aaataggtcc aaatcatcac cattgcaatg gttgaatggc gtggcgcaac
    37261 ggctctgaaa cggtgctggg gtatgatggc aacagagtcg aaagtggatg tgtcactgtg
    37321 gatgctgtcg cctggtgtag cggggaggat gaagttgatc gttcggcggt tcatggcact
    37381 gctgctgttg gcactgctct gcggcattgc ggggtgcagc ctgctgttca gcaagccgaa
    37441 gccgccggag gtcttcatct ccagcattca catcgaatcg ctggcgctgg ccgagcaggt
    37501 gctgctgttc cggctgcggc tgcacaatcc caacgactat ccggtgccga tccgcgagct
    37561 gacctaccgt gtcgagctga acgaccagct ctttgccgag ggtggcagcg ccgaggcggt
    37621 gacgctgccg gcgctcggcg acagtgagat cgagctgcgc gcccgcgtcc gctccaccca
    37681 gctgctgacg cagctctcgc gcatggcgct gcgtgggcag tcgtcggttc gttatcgact
    37741 gaacgggcgg ctgacgctga cccgcggcca tctcggtgtc gatttccggc aggagggttc
    37801 gctctcgctg cccgacctca ggtcgctgcc ccggcggaga tgagtgcaat ggttcgatgc
    37861 gccggaccca cagcgtgcag ccagtggtcg atggccgcca cgatctgctc ccagcccggt
    37921 tcagccacca gccagtgggc atggttcggc aactccagat aggtggccac ccgccggtac
    37981 cgctgtgcga cctgtcgcac cacacggact ggggtgatgc ggtcctcacg gccggcaatg
    38041 atcagcatcg ggcagtcgat gcgggatgca tccagccgtg aggcgtggcg cagatcgagc
    38101 ggccacaggc cgatctcgaa ggtcgcccgg cccgattcgt ggacgaagcg gtcataggtt
    38161 tcacgttgca gcgtcggtgg cagccggtgc agcatcgagt agacggcctc gtcgaagccc
    38221 tgccgtgtcg gccgctgcca gaagccccag cgggtggcaa tgcgccagaa gctcctgatc
    38281 accgaagggg agagggcgag gatgccggcc ggagaggccg gcgccagcag caccaccgcc
    38341 ctggccagtc cgcgactggc cagcatctgc gccagcaggc cgcccatcga gtggccgatc
    38401 agaaccggtt tctgctccaa ctcgccgatc tgccgcgcca gcgcatcggc atagtcgagc
    38461 aggctggcgc agccgagtct cgggtcgggc ggctggcccg gtgcgatgtc gtggaacggc
    38521 agggttgcgg cgaaggtgcg atgaccggcg gcctcgaaca gccgccgata gccggcccag
    38581 taccaggggc cgccccacat gccgtgaatc atgaagagcg tttcagccat ggtgcgggcc
    38641 gatgtgacgg ggcagccagc aggctgactg ccccgtcgag agggattact gggtcgtcag
    38701 cttggtgccg gcatcaggca gtggcgccag cagttgctgg gccgcatcgc gcatgctgtc
    38761 gctctggccg atcagcccgg ccggcagctt gggcagcgcg ctgatcagcg acagcagcag
    38821 cgagcaggca tagggaatcg actgcatcag cagcatgatc gcccatgcca gcagatcgag
    38881 ggtctcggtg ccgaactggc tgattatcgc gccggaggcc agcagcagcg cggtcagcat
    38941 caggctctcc tcgcgtgcgg cgctcagcgc cttcagcagt gcctgggcat ccgcctgctt
    39001 cggcgtgcgg aagaagggct tgtccttgac gaagaaaccg gtcagtaccg ccatgccgat
    39061 ggtgtgggtc agcgccagcc cggccaccgc ggcggcgacc gtctggcgca ggctggtgcc
    39121 gacccgggtg cggtagaggt agatcagctt gccgagcttg aacacgaaca gtgtcagcgg
    39181 cagcaccgag aagatcagca gcggcggatc gatggtcctg ggcgcgagca gcatcgcggc
    39241 cgaccagccg agcgccgcca ggttgaagat cacattgatg ccatccgcga tccatggcag
    39301 ccagccagcc aggaagtggt aacgctgtcc cggcgtcagc ctgccctcgc cgcgcagcag
    39361 ttggcgcagg tgaccacgca tgatctgaat cgagccgaag gcccagcgaa aacgctgttt
    39421 cttgaagtcg gtgaaggtgt cgggcatcag gccgcggcca tagctcttgg ggatgtaggt
    39481 ggcttcgagt ccctgctcga agatgcgcag tcccagttcg gcatcctcgg tgatgcacca
    39541 ctcggcccaa ccgtcgacct gttgcagcgc atccttgcgc accatggtca tggtgccgtg
    39601 ctggatgatg gcgttgcgtt cgttgcgggt gatcatgccg atgtagaaga agccacggta
    39661 ttcggcatgg atcatcgcct tgaaggcatt ctcgtgggcg tcgcggtaat cctgcggcgc
    39721 ctgcacgatg gcgatcttct ccttcagcag ttgcggcacc aggtcgcgca gccagcgcgg
    39781 gtcgaccttg tagtcgctgt cgatcaccgc caccacctcg gcttctgcgg cagtttcgcg
    39841 cagtgcatag ttgagcgcgc cggccttgaa gccggccagc gggtcgacgt ggaagaagcg
    39901 gaaccgctcg cccagttgtt cacagtgggc ggcgaccggt tgccacaccg ccggatcctt
    39961 ggtgttgttg tcgatcacca gcacctcgaa gcggggataa tcgagcttgg cgagcgcatc
    40021 cagcgtctcg atcatcatcg gcgccggctc gttgtaggcc ggcacatgga tcgacaccat
    40081 cggcagcagc gcctcatcca cctcggtcgg cttgaacagc cgacgatgct gttgcagcca
    40141 gagcgcctcg gcccattcgt gggcttcggc aaacagcacc acgatgacgc cgatcatccc
    40201 cagcaccagc agaatgccta ccaccaccga ggtgagggtc tggtactggt tgctgtagtc
    40261 gtagatgatc cagaccaccg cggtggccgc gctgtaggcg acgatcgcca gaaagctgcg
    40321 gccgcggctg cgcaggaacc tgctgtcgat cagcagcagg ccgaaggtga ccagtgccac
    40381 gacgatcgag atgccggcca gcatcggcca gttcggaatc tcgacgatcg gttcggagaa
    40441 ggggaacttc ggctcgcgcc agacgttgta gaccccccag taggcaccga cgccgctctc
    40501 gacgccatgc ttccagggct ggtcgaaggc ctccatcagg tagtaggtgt aattttcctc
    40561 tttggccagc gcaagaaagc ggcgcagaaa aatcgcctcg ttggctgacg aggccaccgc
    40621 gctctgccgg gtgcgaccat tgctcggcca gccgacctcg ccgatgatca ccggcttgtt
    40681 gggaaaggcg gccttgatct tgtcggtctg ctccttgacg tagtcgacgg catgttccag
    40741 cgcgatgccc tcccaatagg gcagcaggtg gatggtgatg aaatcgacgt ggtcggccag
    40801 ttcggggtac ttcagccaga tgtgccaggg ttcggcggtg ctgatcggaa tgtcggttcg
    40861 gccacgcacc tgatccaggt aggcggtcaa ctgctcgatg ctcacctcgc cacgcagcag
    40921 cgattcgttg ccgaccatca gccgcacgac gttggtcgag cggttggcga tgtcgatcag
    40981 ccgtttcagc tcacgctggg tcgcctcggc atcgccactg acccagccgc ccagcgtgac
    41041 gttgatcttg tgcgccttgg cgaggtcggg aatctccccc aggctgccct ccacggtgta
    41101 ggtgcgcact gcatgggtcc ggcccgacag cagcgccaga tcctcgtcaa tctgctcgat
    41161 gctgggataa cgctgatcgg tcggatcctg atcggcgcgg aacggcgcaa aggagaagcc
    41221 ctggatggtc tttggccagg ccggctcggt gtcggggcgg ttcatgccgg cccagaacat
    41281 caatgtgagt gccgcgacgg caatggcgat gatgaagctc tctttgttca ttgatgaagg
    41341 tgtcgctgaa gggggcgttg attgaaattc tcgggccagc cgaaccggtt ttgcaactgc
    41401 ggggtcgaac ggtggcgtac cgcgtgtgac cgatgaaacc gccaaatgtt cactgtcatc
    41461 gagtcgatgg tgaacgggcg gccagccagc tcagcagcag gaaggccagc accagggcga
    41521 aggtggcctg cgccgcgttg tagagcacca ggccgaggat gccgagcatc agcgccagga
    41581 tgccgagtcg acggctgcgc cgcgtcactg ccagcagggc aatgccgagg ctgagccagc
    41641 cgagcagccg ccagtggatg atcaacccca tgccggtgcg cagggtgcag gacagcccat
    41701 gcgccgcctc ggcgcagcgg ttcaccatct cgaccgactc catcaggcca taacgaacgc
    41761 ttgcagcggc ggcataggcg gtgacaccgg cgatcagcat cggcctgagc gccgacagtc
    41821 gctgccccgg gctttcgcgc cacagcggcc aggccagcag cagcgtcacc agcagccaca
    41881 ggtcggcctg caggttgagc ggggtctcgt tcagcaggat gaaaccggcg ctgaaggtca
    41941 gaaagagtgc gatgtggcgc tcgatcagac tggcattggc ggaggtgacc ggacgcctga
    42001 acagccacag ataggccagt gccggcagca cgaaggcctg actgttgaaa ctgaggtagc
    42061 gggcattgaa gacaagtccg gtgttctcga ccagcgcgca gaacagaatt gcggcgcgca
    42121 gccagccatt cagccagggc aggtcgaagc gggtccggtg cgctgtggga tcgagcagcg
    42181 tggcgatcgg caacggtggc gcgatctggc caccggtcag cgcctggatg gtcaacaggt
    42241 agacaccgag gctcagcgcc atgatgaatg cggcccaggc atactcccag ccgttgcgcg
    42301 cactctggct gaactgatcg gcctgcatcg ccagcagcag cgcgcccgcg accagggtgg
    42361 caatgaccag cgcccagcgc gcagtcggca gcctgcgcgg ctgaatcagc agtggcaggc
    42421 agagcgccac ggcgaaggcc gacagcaaca gcagtgtccg ccactgcgca aagtggctga
    42481 ccgcgccgga gagcaccccc ttgtcggccc gatgggtgtc gtagagaccc cagtagccgc
    42541 cggcggcccc ctcgttgatc cgcttccacg gctggtcgaa ggcttcgatc aggttgtact
    42601 gccatccctg ctgttcggcg agctgcacaa agccgcgaat gaagcgagcc tggttggtca
    42661 ggctgggcag ggccccctcg cgcatccggc cggcggaggg ccagccggtc tcgccgatca
    42721 gcagacgctt gccgggaatc tgttctgtga cctcgccgta cacctggctg atgtgggcga
    42781 tggccgtgtc gatgctggcc ggatcatcct cccagtaggg caggatatgg atggtgacga
    42841 agtcggtggc gggagcgacc tcggggtgtt tgagccagaa ttcccagaca tcggcatagg
    42901 tgaccggaac ctcgggcagc gccgccttga cctggcgcag gtagctggcc agttgcgcgc
    42961 cggtcacctc cttgcgcagc agtgtctcgt tgccgacgat gacggcgcgc accacggcgg
    43021 gatgctgttt tgccagtgcg atcacccgtt ccagctcctt gcgggtcgcc actggatcgg
    43081 aactgaccca ggcaccgagc agcaccttca ggccgaactt tccggccagc ttcggcaccg
    43141 cttcgagacc ggtgaccgag taggttctga cgcattcgaa gcgctgcgac agcagcgcca
    43201 gatcctgctc gatgcgccgt tcggagatgg tcaggccatc atcgaggtcg aacggtgact
    43261 gtgccttctc gaaaggcgca taggagacgc actgcaactt ctgtcctgcg gtcagcccgg
    43321 ctggctgcac cgctcgcggc tggccgagca gcgaccacag cgcgacgagc agcaacaggg
    43381 caaggaggta ggcaatgggt ccggacaggc gcgtcatcgg cggcgaattc catgcgggtt
    43441 gaatcgaacg tgtggcattg cgggttgcgc gtgccaaacc gcaggcgcgg attctacacg
    43501 tcgaagacga gctgcggacg gggcagcatc gatttgcgcg ctgagagctc ctgaatcagc
    43561 gccggcaact gttcggccgc aatggcagcg tcgaacagat ggccctgggc gaggtcgcag
    43621 ccctggtcga gcagaaaccg cagttgctgc acgctctcga ccccttccgc agccacggtc
    43681 agccccagcc ggtgagccat cacgatcacc gcttcggtga tggcgacatc ctcctcgcgg
    43741 ttcggaatgc cctggatgaa gcgccgatcg atcttgacgc tgtcgatcgg cagctccttg
    43801 agcagatgca gtgaggagta accggtgccg aaatcatcga tggcgatggt gacgccgagt
    43861 ctgcgcaact gccacagcac ccggttggcg gcttccagtt gctgggagag aaaactttcg
    43921 tggatctcga tcgtcagcag gctggcaggg aagcggtgcc gatcgaggct ggcagcgagc
    43981 gtgtcgatca gggccggatc gagcagttgg cgcaccgaga ggttgatggc catccggtag
    44041 gccttgcccg gctcgctgtt tcgcagccat gcgccttggg caatcgcctc ggccaccgtc
    44101 cagtggccat gctcgatgat caggccgctc tgctccagcg tgccgaggaa tcgctctgga
    44161 ccgagcagcc cttcggtcgg gtggctccag cgactcaacg cctcgaagcc gatcaggcgc
    44221 tgatcggcca gggtgaactg cggctggtag tggagctgga actgctggtc ggccagtgcg
    44281 cttttcagct catcctgtgc gcgcatccgc gccagcgccg accggttcat ctcttcggca
    44341 aagaagcggt agccgttgcg gttctgctcc ttgatgctgt acatcgccag gtcggcgtaa
    44401 cgcatcagtt cggtgctctc ctggccatcg gccgggatca tcgcaatgcc gatgctggcg
    44461 ccgacggtca ctgcgtgccc gttgatcgat accggctgac gcagggcgtc gagaatgttg
    44521 ccggccacct tctcggccgc cttgatgtcc gccttgtgca gcaggatggt gaactcatcg
    44581 ccgccgagcc gtgcgaccga atcctcccgg cgcacatggg ccgtcagacg ctgtgccacg
    44641 ctctgcagca gttgatcacc cgcttcgtgg ccgagggtgt cgttgacctc cttgaagcga
    44701 tcgaggtcga tgaacagcag ggcggcggtc tcatggtggc gccggcagga ttgcaaggtc
    44761 tgttcgagcc ggtccctgaa cagctggcgg ttgggcagtc gggtcaggct gtcgaaaaag
    44821 gcgagctggg tcaggtcggc ctgcgcggcc ttcagctccg agacatcgtg gtagttgacg
    44881 acgatgccct gcacgcccgg gacatcgagc atcggcacga tgtagccctc cacatcgagc
    44941 cagtggccct gtcggtggtg gatgcggacg ttgctgaaac ggtggacagt gccgctctgc
    45001 tgtcgtgcca gattgagctg gctcatgaag agcgccagat cctccggatg cacgatgccc
    45061 tgtatcgaca gcgtgttttg ggtcggtttc gaccagccca gtgcccgata ggtggatgga
    45121 ctcagatagc gcacctgatg gtgcgcatcg agaatggtca ccatgctggg gctgttttcg
    45181 gtgagggcgc ggaacatctg ctccgaatcg atgcgtttgt cgacctctcg ctgtagcgcg
    45241 gcgttgactc gtgagagatc cagatccttc cagaagttga tccgcacggc ccgctggtgt
    45301 tgccacaggt agccggagat ggcgagattg aaggccgcaa gcttcagact gttggcgatt
    45361 gagtcatagg ccatgacatc gggtttatga ccgacgatcc agagatagcc cagactcagc
    45421 agcaggttga gcgcaacggt ccacagcaga cgcagtggtg cgcagagata gaacagggtc
    45481 gtcatcagaa a
//
LOCUS       DAORCQ010000003        43509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_418_length_43509_cov_5.894624, whole genome shotgun sequence.
ACCESSION   DAORCQ010000003 DAORCQ010000000
VERSION     DAORCQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 43509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43509
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_418_length_43509_cov_5.894624"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1498
                     /gene="nuoN"
                     /locus_tag="PKI23_00410"
     CDS             38..1498
                     /gene="nuoN"
                     /locus_tag="PKI23_00410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNN35430.1"
                     /translation="MTIALTDLLPLAPVLITSATAIAVMLTVAFRRNHFWNATLTVVG
                     LNLALLVLLWGLWQGHLANVTELLVVDAYAAFFMVLILIATLGCATLAHVYLNGYHGN
                     REEFYMLLLIAATGGMVLVASNHLAALFIGLELLSVPLYGMVAYTRNQRHSLEAGIKY
                     LVLSAAGSAFILFGMALIYAKSGALSFQGISAYLSPEAILEPLMLIGTGLMVVGLGFK
                     LSFVPFHLWTPDVYEGAPAPVGAFLATVSKIAVFAVFFRFLSVTPASGSEAFYMLLQV
                     IAVLSILFGNLLALNQANLKRMLGYSSIAHFGYLLVAVLAGHAISLEAVLVYLLTYVL
                     ATMTAFGVVALMSRAEKERDADMLADYRGLFWQRPYLAAVLTIALLSLAGIPLTAGFI
                     GKFYVFTAGANAQLWGLLGVVVVGSALSVFYYLKAMAALYMAKPGMRRYEPKFDWGQR
                     AGGIMVSILFALTVVVGIYPEPLLDLIQIFILSSAV"
     gene            complement(1608..2207)
                     /locus_tag="PKI23_00415"
     CDS             complement(1608..2207)
                     /locus_tag="PKI23_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNN35431.1"
                     /translation="MSKTIEQAGMVSLCFASLLAASAQAASVSYLLDQSNELTDGVPY
                     AKVTIDDDAANTLTFTVKPLGVFTPDSNFGVQEFGFNVNGSMATGVTWNLPTDWESSG
                     PKNLSEFGTFDFVVSGKGNARQDPLIFSLHDAGLTLDSFADASTKGYYFAAHIAGFAT
                     LEGQTSAFVAGSTTVPVPAPAWLLGTGLAALSLVRRRND"
     gene            complement(2521..3144)
                     /locus_tag="PKI23_00420"
     CDS             complement(2521..3144)
                     /locus_tag="PKI23_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNN35432.1"
                     /translation="MIKTAIKQTSLASLCAASLLALSAQAASVSYLLNKSNSLPDAPY
                     ATVTIDDLGTSDTIQFTVTPNHAELVPGSNYGVQQFGLNLIGSIASGTTWILPANWSA
                     NVGSYNMSSFGNFEVEVKKAGQGVRQDPLVFSLKNSGLTVNSFAEGSTTEGYYFAAHI
                     AGFTAPVVTGQSTWVGGSTVAPVPVPAAAWLFGTGLAALGLVRRRGA"
     gene            complement(3404..4096)
                     /locus_tag="PKI23_00425"
     CDS             complement(3404..4096)
                     /locus_tag="PKI23_00425"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNN35433.1"
                     /translation="MPAAKKPQTHATRRRQSAADSRKSALIWYGSGVVSGALASLLVG
                     LSLWPVGPAAITAPGIPGDAATAPAAKSHNGPAKPRFDFYTVLHDQEVRVSEDDPVLA
                     GSKNPPTASTPVTPTEPARTAPAVASATPPGKPPTSDKTAESPKPVASETGKERYLLQ
                     VGSFTQPEEADRLRAQLLLLGLQASVQKIPRDGQTWHRVQVGPFSSQEQLNTARRSMS
                     QQQIESIVLKLR"
     gene            complement(4109..5866)
                     /gene="argS"
                     /locus_tag="PKI23_00430"
     CDS             complement(4109..5866)
                     /gene="argS"
                     /locus_tag="PKI23_00430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNN35434.1"
                     /translation="MKESLAALLKTALTQLQNLGELPAELSFDPQLERTRDRQFGDYA
                     SNIAMELAKAARQNPRQLASRIVATLPPSELLLKVEVAGPGFINFHLATARQHAVIDQ
                     ILAQGAGFGRSSLGSGQRVQVEFVSANPNGPLHVGHGRGAAYGAAVASLFAACGFEVT
                     REYYLNDAGRQMDILSISLWLRYLELLGEAIPFPSNGYRGDYVIDIARLLQAEVGNGW
                     YQRAAALFDGLPPDEPAGGDKEAHVDALIERAKQLLGADYGRLFDFGLTHIQREIAAD
                     LAEFGVHHDQWFSERSLTENGEVEAALALLQRQGLLYEAEGALWFRSTRFGDDKDRVV
                     RRENGVHTYFASDIAYHHNKFQRGFDRIVNVWGSDHHGYIARVKAAIAGLGHDPDRLT
                     VQLVQFVILYRGSERVQMSTRSGSFTTLRELRSEVGNDAARFFYVTRKSDQHMDFDLD
                     LAKSQSKDNPVYYIQYAHARIASMVRKLAEQGEACDDIATAEQLTLLSETREQQLVAE
                     LARYPEVIEQAALQCDPHLLAHYLRDLASHFHIYYNGHRVLVEERALRTARIALCRAV
                     QQVIANGLGLLGVAAPSTM"
     gene            6277..6540
                     /locus_tag="PKI23_00435"
     CDS             6277..6540
                     /locus_tag="PKI23_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35435.1"
                     /translation="MSVRPVVLGMMALVLAGCMGNTITPNYSSSNPNLQVGGKQPVDP
                     APQIENAGSFCLEVQEKWHEDGATPDDQALWAKDTLRKVVPCK"
     gene            complement(6617..7594)
                     /locus_tag="PKI23_00440"
     CDS             complement(6617..7594)
                     /locus_tag="PKI23_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNN35436.1"
                     /translation="MLLARVAENLYWMGRYLERAENLARLINVNSHLLLDLPKGIAPG
                     WRPLIEITGNMALYSERQSEFDEVRVLRFLVTADDNPGSILASLYYARENARTIRDII
                     PREAWELINALHHYARENQASALSKRGRFDYLNQIILRNQTLTGCLVGTMNRDAGYTF
                     VSLGQNIERADMTSRFIDVQSANLIDDEQPEEHPRNAIQWISVLKSLSGYQAYRRAMQ
                     VRVRRTAVLRFLLQHREFPRTVRHALAEVAASLRSLPNHDRPLALALRLEQGIENSDV
                     ERLSQQALHRLIDELQIGLGELSQAIAERYFLKPHPTVAEQSQDALDVE"
     gene            complement(7594..9048)
                     /locus_tag="PKI23_00445"
     CDS             complement(7594..9048)
                     /locus_tag="PKI23_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNN35437.1"
                     /translation="MTIDCAGYPNQNLHDELIDEQQQPRPVARALLQHLASFSDDEIG
                     SLIAAANLAIKEMGISFTVYTEAEGSIDRAWPFDLLPRIMAKREWDGIERGLKQRVKA
                     LNLFIDDLYHEQRIIKDGVFPRELLENSQNFRPQCMGVDPPLGIWAHICGSDLVRDRD
                     GTVYVLEDNLRVPSGVSYMLENRQVLKRVFPELFENYNIQPVDDYSSQLFDTLTAISP
                     RPVEFPQVVVLTPGIYNSAYFEHAYLAQQMGCELVEGRDLVVGKDDIVYMQTMAGLKR
                     VDVIYRRIDDLFMDPEAFNPTSLLGVPGLMRAWKAGNVALANAPGAGVADDKVVYAFV
                     PEVIRYYLGEEIALPNVPTWLCMKPGDREYVLDHLAELVIKPANESGGYGMLIGPHAS
                     KKELAQFARLIQDNPRNYIAQPTLSLSTVPTLVDQKIEPRHVDLRPFILSGRQTFVTM
                     GGLTRVALRKGSLVVNSSQGGGSKDTWIVDEENR"
     gene            complement(9123..10010)
                     /locus_tag="PKI23_00450"
     CDS             complement(9123..10010)
                     /locus_tag="PKI23_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNN35438.1"
                     /translation="MKYRVEHRTHYLYGETVSLCHNLAHIIPLNSARQHCLERAIDIA
                     PRPHAYQEHEDYFGNRVIYFSIEEPHTTLTVTIRSLVELHEVSGQLPIEGTLPWDQVR
                     DLLRDAPSPVLRLAQEYRLPSPMIEPLPELADYAQPSFPSGRPLRTAVRDLTRRIHRE
                     FTFDPHFSSVATPLTEVLAHRRGVCQDFAHLAIGCLRSMGLAARYQSGYLETLPPPGK
                     PRLVGADASHAWFSAFDPEEGWLEFDPTNDRTPDHQYITVAQGRDYADVAPLKGVIFG
                     GGKTHQLKVAVDVISLPDG"
     gene            complement(10007..12598)
                     /locus_tag="PKI23_00455"
     CDS             complement(10007..12598)
                     /locus_tag="PKI23_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNN35439.1"
                     /translation="MNSPLNADRGHFSLIASDYAHHAAAHDEMWLPDPAVDGEGQPRD
                     FWRPLIHTLDRLGGAELERRRQEGRQLLRENGVTYNLYNQPEAPRRGWDLDPVPLLIS
                     QQEWQKTETALTQRAELFNLILRDLYGERRLLREGLLPAELVFRHSGFLRPCQSVTVP
                     GPHQLLLYAADLARGADGQLWVMNDRAQAPSGAGYALETRIALARILPNAFRDGQVRR
                     LAQFFRTLRNTLARLAPREREDPRVVLLTAGPYSETYFEHAYLASYLGYNLVVGDDLT
                     VRDSTVWMKSLDGLKPVDVILRRLDDDYCDPLELLENSRLGVPGLLEAARRGKVAIAN
                     PLGSGLLENPGLLPFLPRLARVLLDQELQLPSANSWWCGDEKSRRHVLDNLDRLLIKP
                     INRRIGSIFGPELSRSQLQGWRDRIERQPHLYVGQEPLAGASTPSLVEGRLQPCQVVT
                     RTFLVSDGDGYAVMPGGLTRIAPSHEMLQISNQTGGTSKDTWVLADRPQPHVSLLPPD
                     GLHPSALERQGVLPSRAGENLFWVGRYAERAEFTIRLLRNVALKLDEIEEYQDQSDRE
                     CLYQLLRALTEVTHTHPGFVGEESQARLLAPQEEIFSLVLDEERVGGIGQILRALVQA
                     AFNLRDLWTSDTWRVIDEIEKMLESMQNHPAHELGRLQAQLDRLVTVLLALTGQTTES
                     MTHEHGWMLLDIGRRLERGINLIDLIRTTLVQNQDEIVRYLILERLLSVQESLIIHRW
                     RYRTAPQLATALELLLLDTTNPRALIYQLRLIEQHLTRLPGHNPRHPSKAERITLEVF
                     SQLRLCDTLALATPDETGHQQLDQLLHRLGQGLGQLSVEITQLYFSLNHPQSEQLLSN
                     TLAEQEV"
     gene            complement(12678..16079)
                     /locus_tag="PKI23_00460"
     CDS             complement(12678..16079)
                     /locus_tag="PKI23_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003186377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNN35440.1"
                     /translation="MSIRVAIEHHTHYRFDRPVQLSPHIFRLRPAVHSRTPIEGYSLK
                     IGPAEHFINWQQDPFGNHLARVVFPEKVRELDVRVEVIARLQVINPFDFFYDEYANSF
                     PFDYPPALKKELAPYLEINESGPLLMHWVGQIGREPCRTTDFLVDLNQRLYRDINYTL
                     RMEPGIQRCDETLEKRLGSCRDSGWLLVQILRHLGLAARFVSGYLVQLTADVKSLDGP
                     SGPEQDFTDLHAWCEVYIPGAGWIGLDPTSGLLAAEGHIPLACTASPESAAPVFGYSD
                     ECETEFSFLNRVTRIHEDPRVTRPYSEEEWCSIDQLGDEVEQRLQQQGVQLTMGGEPT
                     FVSIDDMEGAEWNIAADGSHKRVLARNLLDRLSTRFATGSLRHYGQGKWYPGEPLPRW
                     AYNCFWRSDGVPVWHEPRLLADPASAGQHGIHDARTFAEALCQRLQLDGRHLLPAFED
                     TAYYLWREGNLPINLDPAKAALDDEHERRRLLALLDRGLGEPTGYALPLQWDWRRQCW
                     RSGPWQFRRGQLFLAPGDSPMGLRLPLDALPWVDQALRRTTERHPRSLFEPLPELGDI
                     HGEVARRYSYVQHPPDLHPEIHPQAPVDAEEDENGSPQWVEVPHTAICIEVRDGRLCL
                     FMPPITHLEHYLDLIATIEATARALDLPVLIEGYEPPRDPRLVKLAVTPDPGVIEVNI
                     QPAGSWRELVANTEALYEEAHQSRLGTEKFMLDGRHTGTGGGNHVTLGGRTPAESPIL
                     RRPDLLRSLITFWQHHPGLSYLFSGQFIGPTSQAPRVDEGRHETLYELEIAFSQMPTG
                     EVPQPWLVDRLLRNLLVDLTGNTHRAEFCIDKLYSPDSATGRLGLLEFRGFEMPPHAR
                     MSLVQMLLLRSLLLCFWERPYQQPLVRWGTELHDRFLLPHFIWRDLADVAQFLRDARL
                     PFEQKWLEPFWEFRFPHYGSVSIQGIEIELRMAIEPWHVLGEEMALGGTARYTDSSLE
                     RLQVRVSGLTDGRYILACNGRQVPLAGTGTHGEYVAGVRYRAWQPPSALHPTIGIHSP
                     LVFDLIDSWNGRSIGGCTYHVSHPGGLSYERFPVNAYEAESRRVSRFAGFGHTPGVLQ
                     PPPAVGGVSRFEPRGSGVESMKPPPQERNREYPYTLDLRRPTSFQ"
     gene            16308..16763
                     /locus_tag="PKI23_00465"
     CDS             16308..16763
                     /locus_tag="PKI23_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35441.1"
                     /translation="MKAFRAVPLLLLTAAFAIDVHAQCAASTRVAQPERVLTQKLVCA
                     HKGAPDKPGDRWSELHTRNGSGGGTPLIEWARGAQDPVDPSRNVGTWKVQGSNIVYSY
                     TGDGSYTYQLFNDGAGQYSLCDSQGGTVKATIMKLVPSPSGTNPCGWSN"
     gene            16913..17626
                     /locus_tag="PKI23_00470"
     CDS             16913..17626
                     /locus_tag="PKI23_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35442.1"
                     /translation="MTLFNLRQRDFLRRLELPLWQARQPLPGARQTRVEPPAPPEASP
                     SVTLPSPLVDALPTASLADQAATATEFALYHRRLPRGWLLLCDLEAPAQQLAAGPRRL
                     LDNILFALDVEADAPAAEGVLRWPPPLPGLQLDVDPAAAAHFLEAFIAGQQEREGVTH
                     LLCFGAKPLQLLRLAGVAASVELGTQPGQCSAGGMTLVATHALFELLADPPRKAASWR
                     HLRPLLQPGSSRGDDRPSH"
     gene            17604..18089
                     /gene="rimI"
                     /locus_tag="PKI23_00475"
     CDS             17604..18089
                     /gene="rimI"
                     /locus_tag="PKI23_00475"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNN35443.1"
                     /translation="MTTAHRIDPIEIAPLLWSDLVEVVAIDAASDPAPWSLALFEQSL
                     QVGHLGHKALCAGQMVGYSLTQLVQDEAELLRIAVLADFRRAGLGRALLSEVIAVAID
                     AGCRRLLLEVRSGNQAAIALYQQVGFRTVGQRRGYYPALPGGQKREDALLMALDCAPR
                     Q"
     gene            18086..19027
                     /locus_tag="PKI23_00480"
     CDS             18086..19027
                     /locus_tag="PKI23_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNN35444.1"
                     /translation="MSHPPARWRIGVSRCLLGEPVRYDGGHKRNGYVVETLQRHFDLV
                     AFCPELEVGLGVPRPPIRLVRHAQSVRALGVEQPELDVTLPLTRYLDQVRERVAPLVG
                     FILKQGSPSCGLEVALHEAERPIGTTAGLFSGRLRAALPWLPVEEEGRLSDPRLRENF
                     LTRLFVHGRWRDLPDGGLDFATLLAFHRSHKMLLLAHDEACYRRLGRWLATARQLEPG
                     QRAQQYLGQLLQGLERLPTPGSHGNALQHLAGFLKRQLDARQRGELAATIDAYRQGAL
                     PWTTPVALLNHHFQHHPHPFALAQHYLRPLPAELLGP"
     gene            19196..19792
                     /locus_tag="PKI23_00485"
     CDS             19196..19792
                     /locus_tag="PKI23_00485"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017708609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNN35445.1"
                     /translation="MLLMIDNYDSFTYNVVQYLGELGAEVVVVRNDEIDMAGIERLAP
                     SHLVISPGPCTPNEAGISVPAIRHFAGRLPILGICLGHQGIGQAFGGRVVRAGRVMHG
                     KTSLVHHRGQGVFAGLPNPFVATRYHSLVIEQSSLPSVLEVTAWTEDESGRIEEIMGV
                     RHREFSVEGVQFHPESILTEGGHALLRNFLAQQGGRRQ"
     gene            19822..20850
                     /gene="trpD"
                     /locus_tag="PKI23_00490"
     CDS             19822..20850
                     /gene="trpD"
                     /locus_tag="PKI23_00490"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNN35446.1"
                     /translation="MTLPQAIARLVERHDLTAVEMAQVMRLIMTGQASAAQIGAILVA
                     LRMKGETVDEIAAAARVMRELVTPVPIRAANAVDTCGTGGDAAGLFNVSTAAALVVAA
                     AGGKVAKHGNRSVSSKSGSADLLEAAGVRLDLSAVQVARCVDEVGVGFMFAPVHHGAM
                     KHAIGPRRELAIRTLFNLLGPLTNPAGVRRQVLGVFDRHWLRPLAEVLRDLGSDHALV
                     VHAEDGLDEFSLAAPTLVAELRDGEVIEYRVTPQQVGLHGAAQDGLRVGNAEESLALI
                     RAALAGKAALAAELIAFNAGAALYVAGVARDLKEGVMMADDTIATGLALEKLAELVEV
                     TRCMETNA"
     gene            20847..21662
                     /gene="trpC"
                     /locus_tag="PKI23_00495"
     CDS             20847..21662
                     /gene="trpC"
                     /locus_tag="PKI23_00495"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNN35447.1"
                     /translation="MSAPTILDKILARKVEEVAQRRQQVSFGELERLARTAPALRGFA
                     AAIEQKIDAGSAAVIAEIKKASPSKGLIRADFDPPALARSYEAGGAAALSVLTDRDFF
                     QGADEYLQQARAACALPVLRKDFLIDPYQVVESRVLGADCILLIVAALDQSRLLELAD
                     VAREQGLDILVEVHDRAELERALQLDTSLIGINNRDLHSFQTRLETTWALLPLIPAGR
                     RVVTESGIHTDADVAAMRAHGVAAFLVGESLMRADDPGARLAQLFGPLRLVSP"
     gene            21752..21848
                     /gene="ffs"
                     /locus_tag="PKI23_00500"
     ncRNA           21752..21848
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI23_00500"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21938..23596
                     /gene="dnaX"
                     /locus_tag="PKI23_00505"
     CDS             21938..23596
                     /gene="dnaX"
                     /locus_tag="PKI23_00505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNN35448.1"
                     /translation="MSYQVLARKWRPRNFEQTVGQQSVLRALAHALDHDRLHHAYLFT
                     GSRGVGKTSIARILAKCLNCEQGVSSTPCDQCASCVAINEGRFIDLIEVDAASRTKVE
                     DTRELLENVQYTPSLGRFKIYLIDEVHMLSGHSFNALLKTLEEPPAHVKFLFATTDPK
                     KLPLTVLSRCLQLHLKDIPPALIAEHLHRVLDLEGIAHDDGSLKMLGRAANGSLRDAL
                     SLTDQAIAFGGGQLNEADVRQMLGHVDQQSVLQLLQAVVGGNAVQLLDEVEQLAGHGP
                     DYRLVLDELIGLLHQLTLAQVAPGTLHGHWWDGEAVTALAAQMAAEELQLYYQIALHG
                     RRDLPLAPEPRSGFEMVLLRMLAFRPDDGTPDSAAEKKKPLIEPQRSATALLDVPGSP
                     APSSRPIAPAAIAPAPPPPPAAVTPPASQPEATALGEQWIGLCGKLDLSGMSANLAAH
                     AELVAQTAQGWRLRIDPAHSALLDGPHVGRIGEALSRHLGQSVKVEFELGPIQGETPL
                     RSRERQQREARERAIARLEADPKVKALLARFEGSSLDHESIEVG"
     gene            23700..24023
                     /locus_tag="PKI23_00510"
     CDS             23700..24023
                     /locus_tag="PKI23_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNN35449.1"
                     /translation="MKPGIGDLMKQAQKLQEQMQKTQDELANLQVCGESGAGLVKVTM
                     TGRHDVKRVEIDPALLQEEKEMLEDLLAAAVNDAVRRVEEASRDKISSLSAGLNLPGG
                     FKMPF"
     gene            24044..24640
                     /gene="recR"
                     /locus_tag="PKI23_00515"
     CDS             24044..24640
                     /gene="recR"
                     /locus_tag="PKI23_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNN35450.1"
                     /translation="MSELINQLASALRCLPGVGQKSSQRMVMHLLERNRSGGLRLAEA
                     LIRCLNEVGHCTRCRNFTEQPLCAVCADSRRDQSLLCIVETPADMLALEQSGGYRGLY
                     FVLMGRLSPLDGVGADELALDLLWRRLAEEPICEVILATSATIEGEATSHFIAEGLRP
                     DISVTRIAQGVPLGGELEYVDAGTLARALSGRKQFERF"
     gene            24627..25751
                     /gene="rnd"
                     /locus_tag="PKI23_00520"
     CDS             24627..25751
                     /gene="rnd"
                     /locus_tag="PKI23_00520"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNN35451.1"
                     /translation="MNASEPPFVEVSHDAELAQWCERLAGTAVLALDTEFIRVRTYHP
                     QPALLQLSDGRHHLLIDPLTLSRWQPLADCLTDPQQLKVFHAAGEDLEVCRRLFDAVP
                     RPLFDTQVAAAFVGLGFSIGYQRLVEQLLGVTLEKGESRSDWLNRPLTPSQKHYAAQD
                     VIHLHALHQWLHQRLQQRGHLRWFELEMERTLQEAERPILPQERYLHLRAAQPLSSAE
                     LAIARSLAAWREVEAIRRDLPRGFVLPDRALLDLVRRAPNDLSGLSGIAELHPSVIRH
                     HGQTLLQLIAAARTEPADQQPPPLSTQLDAGQKARLQRLKEQVRGRAQQLDIAVELLA
                     SSRELSDWVVSSSDAGAAQPPRRLLEGWRFEAIGDLLTTE"
     gene            25760..26044
                     /locus_tag="PKI23_00525"
     CDS             25760..26044
                     /locus_tag="PKI23_00525"
                     /inference="COORDINATES: protein motif:HMM:NF017018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HNN35452.1"
                     /translation="MGEHQPATPILVEVFRSHKREGAYLYLTRGKGFGHLPAALQGWF
                     RSTDLAMTLWLKPDKKLARVDAAEVLRALDEQGFYLQMPPPREPDPLAPR"
     gene            26178..26771
                     /locus_tag="PKI23_00530"
     CDS             26178..26771
                     /locus_tag="PKI23_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNN35453.1"
                     /translation="MQNPRIKPLPALIFSSRWLQLPLYFGLIAAQGVYVVLFFKELWH
                     LVHGALELNEQQIMLIVLGLIDVVMISNLLIMVIVGGYETFVSRLGIEGHPDQPEWLS
                     HVNATVLKVKLALAIIGISSIHLLKSFIEAGMIGVLPQCSSVAAGVKCATITENGIMW
                     QTIIHLAFILSALGIAWTDRIMSGAVSQHAEKSLSEH"
     gene            complement(26799..27833)
                     /gene="dapD"
                     /locus_tag="PKI23_00535"
     CDS             complement(26799..27833)
                     /gene="dapD"
                     /locus_tag="PKI23_00535"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNN35454.1"
                     /translation="MNSAHFALGFGIGTQDSSGNWLEVYYPAPLLHPAPGLVEALNST
                     LGQASDNRLIAMDAAGWRALATALSQQQQDESARLADQLAASHRPGCVMLLAEDSPPT
                     CVPEAYLKLHLLSHRLVKPHGINLQGLFGVLPNVAWSSEGAIAVSELPQRQLAARIAG
                     RTLVVDAVDKFPRMSDYVVPKGVRIADASRVRLGAYLGEGTTVMHEGFINFNAGTLGE
                     SMVEGRISAGVTVGKGSDLGGGCSTMGTLSGGNSVVISVGEKCLIGANAGLGIPLGDR
                     CTIEAGLYLTAGSKVLLISADGEQRLIKARELAGQSDLLFLRNSQSGAIECRTNRRSI
                     ELNAALHLNS"
     gene            complement(27897..28253)
                     /locus_tag="PKI23_00540"
     CDS             complement(27897..28253)
                     /locus_tag="PKI23_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HNN35455.1"
                     /translation="MQLPTDVVLHGIKSCDSCRAALRWLRDHGVAHQFRDLRQDAPQR
                     TELVVWLERLGPELLNRKSSSWRQLPESLRIDVDADRTIELLLGNPTLMKRPLLEWSG
                     GLLLGFDPDLYQRQLR"
     gene            complement(28253..29455)
                     /gene="dapC"
                     /locus_tag="PKI23_00545"
     CDS             complement(28253..29455)
                     /gene="dapC"
                     /locus_tag="PKI23_00545"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNN35456.1"
                     /translation="MNPRLDLLHPYPFEKLRALLDGVTPPAALKPISWSIGEPKHPAP
                     PFVLETVRQHLDELGLYPPINGIVELREAIAAWAGRRFDLPAGSLTAERHVLPVAGTR
                     EALFSFAQAVINSERSNPLVLMPNPCYQIYEGATLLAGATPRYLASSSATGGLPDLDQ
                     VDAASWNRCQLFYLCSPDNPTGAVASLDYLCHLITLAERHDFVIAADECYSELYFDEA
                     APPPGILQAAARLGLTDYARVIAFHSLSKRSNLAGLRSGFVAGDARLIERYRLYRTYH
                     GAALPVPVQRASIAAWQDEAHVVENRARYRQKFAAVLDILRGSLDVVRPGGGFYLWPR
                     VPLDDQQFTRELYRQQKLTVVPGSFLSRDVNGDNPGACHVRLALVAELDECIEGAERL
                     RRFVEGLG"
     gene            complement(29507..32209)
                     /gene="glnD"
                     /locus_tag="PKI23_00550"
     CDS             complement(29507..32209)
                     /gene="glnD"
                     /locus_tag="PKI23_00550"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNN35457.1"
                     /translation="MNAPLAASRLEQQLDQLLDALTPESPNLIKALRTALDDADALLH
                     EAFRQQFDIDHLVSVRAHFIDRLILIAWRASGLQASADEGALLLVAVGGYGRAQLHPH
                     SDIDLLILLEQEDQQQLQSAITHFVTLLFDIKLNVGYSVRSLNECVQQARLDVTVLTN
                     LIESRVLDGEPELMLRLHVALGPDQMWPPAEFFRAKFTEQQRRHEKYYDTEFNLEPNL
                     KGSPGGLRDLQTIHWIAKRLLGARQDQDLIALGLLSPDEFNQLEAARRMLWRIRWGLH
                     MLARRAEERLLFEHQRTLAELFGFHDDPNKLSVENFMQEYYRTAQNVSDFNEILLQHI
                     EEGVLRQIDHQPIEIETLNERFRLRNNFIEVSRSTLFQEEPRTLLEIFVILGNRPDIQ
                     GVRATTIRLIRRHSHLIDEALRNDPQVTALFMELLRSPHFLVTQLQRMQRYGILGRYL
                     PEFGRVIGQMQHDLFHIYTVDAHTLLLIRNLRRFKNGSTEAQYALVSMIVPNIEKYEL
                     LYIAGLYHDIAKGRGGDHSTLGASDAEAFCQRHGLSQRDTNLVVWLVQSHLLMSMTAQ
                     RCDISDPETIHHFASQVRDLQRLDYLYLLTVADINATNHVLWNSWRASLMRQLYTETR
                     RALRRGLENMVDKQERITETQSEALQLLQQAEVSLSAAQEIWRNLGEDYFLRESAADI
                     AWHTAAIHAHATLDRPLVLIKETPSRQFDEATQLFIYTRNQANLFAAIAAAMEQLSLS
                     IQDARIITSDNDMALNTYYVLDEEGRPLINDRRLLQQITQRLTQDLADPTLYPHVVRR
                     HVRRQLKQFPIPTEVLLYTDAIKNHTVLEVITADRPGLLARIGQIFMEFDIYLLKAKI
                     ATLGERIDDVFFITDKNRQPIADPVICQRLKQTLCAQLDAQSQG"
     gene            complement(32220..32996)
                     /gene="map"
                     /locus_tag="PKI23_00555"
     CDS             complement(32220..32996)
                     /gene="map"
                     /locus_tag="PKI23_00555"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNN35458.1"
                     /translation="MKSTLIKRPDEIEKMRVAGRLAAEVLEMIAPHVQPGVSTGELDR
                     ICHDHIVNVQKAIPAPLNYNGFPKSICTSVNHVVCHGIPSDDKILKNGDCINLDITVI
                     KDGYHGDTSMMFFIGEPSILNRRLVQVAQECLYRAIGLLRPGVRLGDIGHVIQTHAES
                     NHFSVVREYCGHGIGKIFHEEPQVLHYGTPNTGMALEPGMTFTIEPMLNAGKRHVTLL
                     PDQWTVITKDRKYSAQWEHTLLLTEDGCEVLTLRQGEVIE"
     gene            complement(33073..34590)
                     /gene="gltX"
                     /locus_tag="PKI23_00560"
     CDS             complement(33073..34590)
                     /gene="gltX"
                     /locus_tag="PKI23_00560"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNN35459.1"
                     /translation="MTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFLLRIEDT
                     DRERSSSSSEQRIFDALRWLGLDWDEGPDVGGPHGPYRQSERGLIYQLHVRQLLEQGH
                     AFHCFCSEERLTELRKRQLVLQEQPGYDGHCLQLSQAQVQSRLAAGETAVVRMVVPRT
                     GRCQFDDLLRGSIEIDWQQVDMQILQKSDGMPTYHLANVVDDHLMQITHVIRGEEWIN
                     SVPKHLLLYQYFGWQPPVMCHLPLLRNPDKSKLSKRKNPTSIDFYRRMGFLPEVLVNY
                     LGMMGWSMPDGRDKFSLAEMIEAFDLARVSLGGPVFDIDKLKWLNGLYLREEYDTDRF
                     IALYRQWAFDDDYCKRIVPEIRSRIELLSDVVEIAGHFVAGHPRIDASHFSHKSLEIE
                     AIRKILQFGLWQLERLRDWNKEEIHCTIEQLSRAMGHKIRDFLFPFFIAIAGSAASSS
                     VLEAMAILGPDLSRARLRRAIEILGGVSKKESKRWEDELAALVEAMQQAAPPSAE"
     gene            34799..35869
                     /locus_tag="PKI23_00565"
     CDS             34799..35869
                     /locus_tag="PKI23_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNN35460.1"
                     /translation="MNFSAQLLSLEQNPHILFSVPSPMKQHLVPSRFSALNLTRAKRS
                     LHLCLGLLLLFGVNLPLGASARESYLAQSNNRPVDLVGDIRKVQANHEDTLLDIARRN
                     GAGYWDMVRANPDVDPWLPGKGTLVVVPSRHILPRTARDGLVINIPELRLYQYYRDGQ
                     GRQWVRSYPISVGRSDWKTPVGTTRVVAKRENPAWTPTESVRKEHAARGDILPKVVPA
                     GPDNPLGRYAMNLGFESYLIHGTNRPFGIGMQVTHGCMRLYPEDIEELFKSTAVSTPV
                     HLVNEPVKLGWSSNSLFLEVHLNEEQKMDRKLYYSLIDQTLKLLQQTLGDIPPGMDLS
                     RVKAVVEQANGLPRLIWTRPTT"
     gene            complement(35978..36268)
                     /locus_tag="PKI23_00570"
     CDS             complement(35978..36268)
                     /locus_tag="PKI23_00570"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HNN35461.1"
                     /translation="MSNTKKSLTFATVALGSLLLLGGCSSNEKIDQAAADAQKALAAA
                     QEASATASAADRKADQALSAANQAQATANEALSKANATDEKLSRMYKKSMSK"
     gene            36541..37395
                     /gene="purU"
                     /locus_tag="PKI23_00575"
     CDS             36541..37395
                     /gene="purU"
                     /locus_tag="PKI23_00575"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNN35462.1"
                     /translation="MSHSYRLILGCPDRVGIVAAVSRFIAERDGWIIEADQYSDPASH
                     HFFMRVVIRAESLNCPLDQFRRDFVEVAARYEMNWRLHDSAQRQRVAILASRESHCLY
                     DLLHRWKDGELLFDLPCVIGNHDHLAEAVEWHGVPFYHVPMEPARKQAGFDRIHLLLQ
                     QHRVDVVVLARFMQILPNVLCQHYAGQIINIHHSFLPSFIGAKPYHQAAERGVKLVGA
                     TCHYVTEALDAGPIIEQDVVRVSHHDTIEDMIRLGRDVEKQVLARGLRYHLEDRALIH
                     GNKTVIFA"
     gene            complement(37435..37899)
                     /locus_tag="PKI23_00580"
     CDS             complement(37435..37899)
                     /locus_tag="PKI23_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNN35463.1"
                     /translation="MTLDRHDRAILTLLQHQGRISNQELADQVGLSPSPCLRRVRSLE
                     QNGIISGYRALLDAKRLGLTLMALIHISMDRHTPERFENFESKIREIPHVVECLLITG
                     QEADYQLKVLVHDMDAYQTLLLNRITRIDGVSGVHSSFVLRSVVDRTELPLE"
     gene            38108..39775
                     /gene="leuA"
                     /locus_tag="PKI23_00585"
     CDS             38108..39775
                     /gene="leuA"
                     /locus_tag="PKI23_00585"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNN35464.1"
                     /translation="MSQFDHRKYRPIAAIDLPHRQWPSRSFTKAPKWASVDLRDGNQA
                     LLEPMNVMQKQRMWALLVKLGFKEIEVGFPSASQLDYDFVRWLIEAEQIPDDVTVQVL
                     VQAREDLIVRTFEALVGVRRAIVHVYNSTSPAQRERVFGLDRAGVTEIARQGALWVQR
                     EAANHPQTDWCFQYSPESFSNTEVDYAVEICEAVLDVWQPTPERPCIINLPSTVEVSS
                     PNLFADQVEYFATHISRRDSIILSVHTHNDRGCAVAAAELAVLAGADRVEGTLLGNGE
                     RTGNMDIVTMAMNLYSQGIDPELDLSNPDEIIEVYIECNNLPIHPRHPWIGELVYTAF
                     SGSHQDAIRKCLHLQKPDEPWQVAYLPIDPRDIGRDYQAVVRVNSQSGKGGVAFVLER
                     DYGLILPRWMQVELAQQVQAQSERQGGIVESAAIYQLLLDHFVNDQSPVRLLGYRLDR
                     NGHDTIEARLNEAGQNRSLLGSGDGAISAFIDAWSRHSQQQVSVVDYHEHAVGTGTDA
                     QAIAYVQLSIEGRRVSGVALDQDTVSASLKAVLSALNRYWGLSRRVA"
     gene            40193..41413
                     /locus_tag="PKI23_00590"
     CDS             40193..41413
                     /locus_tag="PKI23_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNN35465.1"
                     /translation="MAGIIIVGAGQAAVQAAASLRQEGCKDEITIFGDEPWLPYQRPL
                     LSKQYLLGTETEEKIYQRAEKFYEDQRVALRLGERIESIDVAAKTVTSAKGEVIPYKE
                     LLLATGSRPRILKIQGSELSGIHYLRTLGDVNAIKNEMAPGKRLVIIGGGYIGLEVAS
                     VAVTLGLDVTVLEMEERILKRVTTAEMSEFYHQLHAGRGVKILLRTAVSGFEGDGHIT
                     HVLCGDQRIAADMVIVGIGIIPNVELAQAAGIECNNGIVVDDRCRTSAEHVYALGDCS
                     NHPSPLVGRRLRLESVPNAQDQAKVVAANIMGKPKIYSAVPWFWSDQYELKLQMVGFS
                     ADGDTQVLRGDKSTNQFTVFYLKEGKLVAVDAVNNPRDFMIGRQFYGRKMDVSRLSDL
                     SVDVKDLVLPVSAG"
     gene            41521..41841
                     /locus_tag="PKI23_00595"
     CDS             41521..41841
                     /locus_tag="PKI23_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNN35466.1"
                     /translation="MSKVTFNLFNGERQTYDAADGVSVMQVGKDHNVPGIDADCGGEC
                     NCATCHVYVDEKWMPKVGMPHDAEEAIMSLNPDRKPNSRLSCQIKMSADLDGLVVNLP
                     EFRY"
     gene            41927..43186
                     /locus_tag="PKI23_00600"
     CDS             41927..43186
                     /locus_tag="PKI23_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNN35467.1"
                     /translation="MTTAVQKKYDHMYGKDPYSIPLEDYDVSQPELFESNTMWGFFDR
                     LRKEDPVHYCKDSENGPYWSVTKFKDIMEVEGNPEVYSSEPTISLVDPREDFKVSMFI
                     AMDEPRHGEERRTVQGVVAPANLARMEPLIRERVCGILDSLPVNETFNWVDKVSIELT
                     TQMLATLFDFPFEQRRKLTYWSDVATTIPAPGALVESIEERQGILINECLPAFTKLWN
                     ERVNAPPQFDLISMLAHGESTRHMQPYEFLGNLMLLIIGGNDTTRNSLSGGVLALNEN
                     PEQYQKLRDNPGLIPKLVPEIIRWQTPLTFMRRTATQDTILRGKRIKKGDKVVMWYVS
                     GNRDEEVIDRPYEFIIDRENPRHHLSFGFGIHRCMGNRLAEMQLRVVWEEVLKRFHKV
                     EVVGKPVRNYSPFIKGFSDLPVQLRPL"
BASE COUNT         7690 a        12885 c        14410 g         8524 t
ORIGIN      
        1 atgccgccgc cattcaagct gccgaggccg ctgcgccatg accattgccc tgaccgatct
       61 gctgccgctc gcgccggttc tgatcacctc ggccacagcc atcgcggtga tgttgaccgt
      121 tgcctttcgc cggaatcatt tctggaatgc caccctgacg gtcgtcgggc tgaatttggc
      181 gctcctcgtg ctgctgtggg ggctgtggca gggtcatctg gccaatgtga ccgaactgct
      241 ggtggtcgat gcctatgcgg cgttcttcat ggtgctgatc ctgatcgcga ccctcggttg
      301 cgccaccttg gcccatgtct atctgaacgg ctatcacggc aaccgcgaag agttctacat
      361 gctgctgctg attgccgcca ccggtggcat ggtgctggtg gccagcaacc atctggccgc
      421 actgttcatc ggtctggaac tgctgtcggt gccgctctat ggcatggtcg cctacacccg
      481 taaccagcgc cattcgctgg aggcgggcat caaatacctg gttctgtcgg ccgccggctc
      541 ggccttcatc ctgttcggca tggcgctgat ctacgccaag agcggtgcac tgtcgttcca
      601 gggcatcagt gcctacctgt cgcccgaggc gattcttgag ccgctgatgc tgatcggtac
      661 cggcctgatg gtggtggggc tgggcttcaa gctctctttc gtgccctttc atctgtggac
      721 gcccgatgtc tatgagggtg cgcccgcgcc ggtcggcgcc tttctggcca ccgtcagcaa
      781 gattgcggtg ttcgcggtct tcttccgctt tctctcggtg acgcccgcca gcggcagcga
      841 ggccttctac atgttgctgc aggtcatcgc agtgctgtcg atcctgttcg gcaacctgtt
      901 ggcgctgaat caggccaacc tcaagcggat gcttggctac tcgtcgatcg cccacttcgg
      961 ctacctgctg gttgccgtgc tggcggggca tgccatcagt ctggaggcgg tgctggtcta
     1021 tctgctcacc tatgtgctgg ccaccatgac ggcctttggc gtggtggcgc tgatgtcgcg
     1081 ggccgagaag gagcgcgatg ccgacatgct ggctgactac cgcggactgt tctggcaacg
     1141 gccttatctc gccgccgtgc tcaccattgc gctgctgtca ctggcgggca ttccgctgac
     1201 ggccggtttc atcggcaagt tctatgtctt caccgccggc gccaacgccc agttgtgggg
     1261 actgctgggg gtggtggtgg tcggcagtgc gctgagcgtc ttctactacc tgaaggcgat
     1321 ggcggcactc tacatggcca agccgggcat gcgccgctac gagccgaagt tcgactgggg
     1381 gcagcgcgcc ggcggcatca tggtgtcgat tctgttcgcg ctgaccgtgg tggtcggcat
     1441 ctatcccgag ccgctgctcg atttgatcca gatcttcatt ctctccagcg cggtctgaga
     1501 cgccagcggc gctcgatgaa aggggtggcg tctcggacgc cacccctttc attttccaga
     1561 tccatctgat tggggatcag cttggtgagg cccagcgaac agttgattca atcgttacgg
     1621 cggcgcacca ggctcagagc tgccagacca gtgccgagca gccaggcggg ggctggaacc
     1681 ggcaccgtgg tcgagccagc gacgaaggcg ctggtttgcc cttccagggt ggcaaaaccc
     1741 gcaatgtgcg cggcgaagta gtagcctttg gttgaggcat cagcgaagct gtccagcgtc
     1801 aacccggcat cgtgcaggct gaagatcagc ggatcctgac gcgcgttgcc tttcccgctg
     1861 acgacgaagt caaaggttcc gaattcgctg aggttcttcg gcccgctgga ctcccaatcg
     1921 gtgggcagat tccatgttac gcccgttgcc atgctgccgt tcacgttgaa gccaaactcc
     1981 tgcacgccga aattgctgtc gggtgtgaag actccaagcg gcttgacggt gaaggtgagg
     2041 gtgtttgcgg cgtcgtcgtc gatggtgacc ttggcatagg gcacgccatc ggtcagttca
     2101 ttcgactgat ccagcagata gctgaccgag gcagcctgcg cgcttgccgc gagcagggag
     2161 gcgaaacaga gtgagaccat tccggcctgt tcgatggttt tgctcattgc attgctcctg
     2221 atacttcaag ttcagcgggc aaccaaaggg gcaggaccgc ctttggatca ggacgggatt
     2281 gcaagcactc ctgtcacacc catggccagc aatgaaagcg ccaattattt tttctttttt
     2341 taaatcaata atatggataa atgaagcgaa aacatgcaac tggaatgatg cgaaccggca
     2401 cgcaatatcg atattttggg gtgcacgggc agaagtcctg cctctttgtc gatcacgctg
     2461 ttgcatggtg gatcctcagg cagattgctc tgcctgagga catcccaagc gcccgaactc
     2521 tcaggcgccg cggcggcgca ccaggcccag ggcagccaag ccagtaccga acagccaggc
     2581 tgcggccgga accggaaccg gagccaccgt cgagcccccg acccaggtgc tttgtccagt
     2641 caccacgggg gcagtaaagc ccgcgatgtg cgcggcaaag taatagcctt cggtcgtcga
     2701 accctcggcg aagctgttca ccgtcaggcc ggaatttttc aggctgaaga ccagtggatc
     2761 ctggcggaca ccttgacccg cttttttgac ctcgacctca aagttgccga atgaactcat
     2821 gttgtatgat ccaacattgg cgctccagtt ggctggcagg atccaggtcg tgcctgaggc
     2881 aatgctgccg atcaggttga ggccaaactg ctgcacgccg tagttggatc caggcacgag
     2941 ttcggcatgg ttcggcgtga cggtgaactg gatggtgtcg gacgtgccaa ggtcgtcgat
     3001 ggtgaccgtg gcatatgggg catcgggcag cgaattcgat ttgttcagca gatagctgac
     3061 cgaggcagcc tgtgcgctca gcgcgagcag cgaggcggcg cagagcgagg ccaggctggt
     3121 ctgtttgatg gcggttttga tcattgcaca actcctgaga cttcaattga aggggatggg
     3181 agaggaatca cttctctcct aaaaatttaa agcaatgatt ggtccaatta tttttttatc
     3241 tttaaaatca tattattaaa aatatatcaa aaaagtatta gcactaaaaa cattcttttt
     3301 gtaaaaaaat ggacaatttc tctctggcgg ggaacggtgt cgagtctttc gggaagggga
     3361 gagtgatccg gggtcggcgt catgcgcgcc gggaggtgtg cagtcagcgc agcttcagca
     3421 cgatgctctc gatctgctgc tggctcatgc tgcggcgggc ggtgttgagc tgctcctgcg
     3481 atgagaatgg gccgacctga acccggtgcc aggtctgtcc gtcgcgcgga atcttctgga
     3541 ctgacgcctg caggccgagc agcagcaact gggcgcggag ccggtcggcc tcttcgggct
     3601 gggtgaagga gccgacctgc agcagatagc gctccttgcc ggtttcgctg gccacgggtt
     3661 tggggctctc cgcagttttg tcgctggttg gcggtttgcc gggaggcgtg gcgcttgcga
     3721 cggcgggtgc cgttctggcg ggttcggtgg gcgtgaccgg cgtactcgcg gtgggcgggt
     3781 ttttgctgcc tgccagcacc ggatcatctt ccgagaccct gacttcctga tcatgcagca
     3841 cagtgtagaa atcgaagcgc ggtttggcgg ggccgttgtg gctctttgcc gctggtgcgg
     3901 ttgcggcatc acccggtatg ccgggtgcgg tgatggctgc cgggccgacc ggccagagcg
     3961 acaggcccac cagcagcgaa gccagcgcgc cgctgaccac gccgctgcca taccagatca
     4021 gcgccgattt gcgtgaatca gcggccgatt ggcggcgtcg ggtggcgtgg gtttgcggtt
     4081 ttttggcggc aggcatggcg attccgcgct acatggtact gggggctgct acaccgagca
     4141 gtcccagacc gttggcgatg acctgctgca cggcgcggca gagggcgatg cgggcggtgc
     4201 gcagggcgcg ctcttcgacc agcacccgat ggccgttgta gtagatgtga aagtggctgg
     4261 ccaggtcgcg caggtagtgg gcgagcaggt ggggatcaca ctggagcgcg gcctgttcga
     4321 tcacctctgg atagcgcgca agctcggcaa ccagctgctg ttctcgcgtt tcactcagca
     4381 gcgtcaactg ttcggcggtg gcgatatcgt cgcaggcctc gccctgttcg gccagtttgc
     4441 gcaccatgct ggcgatgcgg gcgtgggcgt actggatgta gtagaccggg ttgtccttgc
     4501 tctgtgactt ggccagatcg aggtcgaaat ccatgtgctg gtccgacttg cgcgtcacat
     4561 agaagaagcg cgccgcatcg ttgccgacct cgctgcgcag ctcgcgcagg gtggtgaaag
     4621 agccgctgcg ggtcgacatc tgcactcgct cgctgccgcg atagagaatc acgaactgca
     4681 ccagttgcac ggtgagccgg tcggggtcat gaccgaggcc ggcgatcgcg gccttcaccc
     4741 gggcgatgta gccgtggtgg tcggagcccc agacattgac gatgcggtcg aagccgcgct
     4801 gaaacttgtt gtggtggtag gcgatgtcgg aggcgaaata ggtgtggacg ccattctcgc
     4861 ggcggaccac ccgatccttg tcatcaccga accgggtgga gcggaaccag agcgcgccct
     4921 cggcttcgta gagcagcccc tgacgctgca gcagcgccag cgcggcctcc acctcgccat
     4981 tttcggtcag gctgcgctcg gagaaccact ggtcatggtg gacgccaaac tcggccagat
     5041 cggcggcgat ttcacgctgg atgtgggtca gtccaaaatc gaacagccgg ccatagtcag
     5101 caccgagcag ctgtttggcg cgctcgatca gtgcatcgac atgcgcctcc ttgtcgccac
     5161 cggccggttc gtcgggcggc aggccatcga acagggccgc tgctcgctgg taccagccat
     5221 tgccgacctc ggcttgcagc aaccgggcga tgtcgatgac gtagtcaccc cgatagccgt
     5281 tgctgggaaa ggggatggct tcacccagca gctccagata gcgcagccag aggctgatgg
     5341 agaggatgtc catctgccgg ccggcatcgt tgagatagta ctcgcgggtc acctcgaagc
     5401 cgcaggcggc aaagaggctg gcgactgccg cgccataggc ggcgccccgg ccgtggccga
     5461 catgcagcgg gccattgggg ttggccgaga cgaattcgac ctgcacccgc tggccgctgc
     5521 cgaggctgga gcggccaaac ccggcaccct gcgccagaat ctggtcgatc accgcatgtt
     5581 ggcgggcggt cgccaggtga aagttgatga agccagggcc ggccacttcc accttcagca
     5641 gcagctcact gggtggcagc gttgccacga tgcggctggc cagttggcgg gggttctgcc
     5701 gtgccgcctt ggcgagctcc atggcgatgt tgctggcata gtcgccaaac tggcggtcgc
     5761 gggtccgctc cagttgcggg tcgaaggaca gttcggcagg cagctcgccg agattctgga
     5821 gctgtgtgag ggcggtctta agcagtgcgg cgagggattc tttcatgaag gagcgtgaaa
     5881 ctgcggaagg ttctccggcc aaaaccggct cgcaacggac tgcgaaagag cagccattat
     5941 gccagatcga ccagatcggc caaagcgcgc gtggcggcgg cagaactcag cttcagaacc
     6001 ctctggtcgc gcgtcgccac tgcagtcatc cgggcgcaat tcacccactg ccacgaaacg
     6061 ctacgcgtca tccgtgccat cgcttcggtc tgctctcctc ggcaagccgg ctcagcaaaa
     6121 actgtccaga gtgccaaagg tgctggacca acaccttgaa ttgacacttt ttactgggcg
     6181 cctgaaaatg cgcgcccgct cgggcagcgc ggtttcgacc gtggcccgct ccactgtttt
     6241 gattcgtcgt cgcgcttctg gcgccaggag ggtttgatgt ccgttcgtcc cgttgtattg
     6301 ggcatgatgg cgttggttct ggccggttgc atgggcaaca ccattacccc gaactacagc
     6361 agctcgaacc ccaacctgca ggtgggtggc aagcagccgg tcgatccggc accgcagatc
     6421 gagaatgccg gctctttctg tctcgaagtg caagagaagt ggcatgaaga tggcgcaaca
     6481 ccggatgatc aggcgctgtg ggccaaggac accctgcgca aggtggttcc ctgcaagtag
     6541 gacttgcagg gcaggtttgc ctgtcggtac atgacggccg ctctgttctg gagcggccgt
     6601 ttttttggcg agtcgctcac tccacgtcca atgcatcctg cgactgttcc gcgacggtcg
     6661 gatgtggctt gagaaagtag cgctcggcga ttgcctgact cagttccccc aacccgatct
     6721 gcaactcgtc gatcagccga tgcagtgcct gctgcgacag ccgttcgacg tcgctgttct
     6781 cgatgccctg ttcgagtcgc agcgccagcg ccaatggccg gtcatggttg ggcaggctgc
     6841 gcaggctggc cgcgacttcg gcgagggcat ggcgcacggt gcgtggaaat tcccgatgtt
     6901 gcagcagaaa acgcagcaca gcggtgcgac gcacccgcac ctgcatggcc cgccgatagg
     6961 cctgataacc cgagagtgat ttgagcacgc tgatccactg gatggcgttg cggggatgct
     7021 cctcgggctg ttcgtcatcg atcaggttgg ccgactggac gtcgatgaag cggctggtca
     7081 tgtcggcgcg ctcgatgttc tgccccaggc tgacgaaggt gtacccggca tcgcggttca
     7141 tcgtgccgac caggcagccg gtgagggtct ggttgcgcag gatgatctgg ttgaggtagt
     7201 cgaaccgtcc ccgtttggag agggcgctgg cctggttttc tcgggcatag tggtggagcg
     7261 cgttgatcag ctcccaggct tcgcgcggga tgatgtcgcg gatggtgcgg gcgttttcgc
     7321 gcgcgtagta gaggcttgcc agaatcgacc ccgggttgtc gtcggcggtg accagaaagc
     7381 gcagcacccg cacttcgtcg aattcgctct ggcgctcgct gtagagcgcc atgttgccgg
     7441 tgatctcgat cagcggccgc cagccggggg cgatgccctt gggcaggtcg agcagcaggt
     7501 gactgttgac attgatcagc cgtgccaggt tctcggcccg ctccaggtag cgacccatcc
     7561 agtaaaggtt ttcggcaacg cgtgcgagca gcatcagcgg ttctcctcat cgacgatcca
     7621 ggtgtccttg ctgcccccac cctgcgagga gttgaccacc agtgagccct tgcgcagtgc
     7681 gacccgggtc aggccgccca tggtgacgaa ggtctggcgg ccggagagga tgaacggacg
     7741 caggtcgaca tggcgcggtt cgatcttctg atcgaccaga gtcggcacgg tggagaggct
     7801 gagggtcggc tgggcaatgt agttgcgcgg gttgtcctga atcagtctgg cgaactgggc
     7861 cagctccttt ttgctggcgt gggggccgat cagcatgccg tagccgcccg attcgttggc
     7921 cggtttgatc accagctcgg ccagatggtc gagcacatac tcccggtcgc ccggcttcat
     7981 gcagagccag gtggggacgt tgggcagcgc gatctcctcg cccagatagt agcggatcac
     8041 ctcggggacg aaggcgtaga ccaccttgtc gtcggcgaca ccggcgcccg gggcgttggc
     8101 gagggcgaca ttgcctgcct tccaggcgcg catcaggccg ggaacgccga gcagcgatgt
     8161 cgggttgaac gcctccggat ccatgaacag gtcgtcgatg cggcggtaga tcacatcgac
     8221 ccgtttcagt cccgccatcg tctgcatgta gacgatgtca tccttgccga ccaccaggtc
     8281 gcgtccttcg accagttcgc agcccatctg ctgggcgagg taggcgtgtt cgaagtaggc
     8341 ggagttgtag atgcccggtg tcagcaccac cacctgggga aactcgaccg ggcgtggcga
     8401 gatggcggtc agggtgtcga acagttgcga gctgtagtcg tcgaccggct ggatgttgta
     8461 gttctcgaac agttcgggaa acacccgctt cagcacctgg cggttctcca gcatgtagga
     8521 gacgccggag ggcacccgca ggttgtcctc cagcacatag acggtgccgt cgcggtcgcg
     8581 gaccaggtcg gagccgcaga tgtgggccca gatgccgagc ggtggatcga cccccatgca
     8641 ctgcggacga aagttctgcg aattttcgag cagctcacgc gggaagaccc catctttgat
     8701 gatgcgctgt tcgtggtaga ggtcgtcgat gaagaggttg agcgccttga cccgctgctt
     8761 gaggccacgc tcgatgccat cccactcccg cttggccatg atgcgtggca gcaggtcgaa
     8821 gggccaggcg cggtcgatcg agccttcggc ctcggtgtag acggtgaagc tgatgcccat
     8881 ctccttgatg gccaggttgg cggcggcgat caggctgccg atctcgtcgt cgctgaacga
     8941 ggccaggtgt tgcagcaggg cccgcgccac cggccgcggc tgctgctgtt cgtcgatcag
     9001 ctcgtcatgg agattctggt tggggtagcc ggcgcagtcg atggtcatgg gagtcgtcct
     9061 gaatctcggt ctctgtttct gcgagagcaa atcatgggcc actgttgcgg tgcgcgctgc
     9121 attcacccgt cgggcaggct gatcacatcg accgcgacct tcagttgatg ggtcttgcca
     9181 ccgccgaaga tcaccccctt gagcggcgcg acatcggcat agtcacgccc ctgggcgacg
     9241 gtgatgtact ggtgatcggg tgtgcgatcg ttggtcgggt cgaactccag ccagccctcc
     9301 tcggggtcga aggcactgaa ccaggcgtgg gaggcatcgg ccccgaccag tcgtggtttt
     9361 ccaggcggtg gcagggtttc gaggtagcca ctctggtaac gggcggccag ccccatcgat
     9421 cgcaggcagc cgatggcgag gtgggcgaaa tcctggcaga caccacggcg gtgggccagc
     9481 acctcggtca gtggcgtggc caccgaactg aagtgcggat cgaaggtgaa ctcccgatgg
     9541 atgcggcggg tcaggtcacg cacggcggtg cgcagtggac gtccggaggg gaacgagggc
     9601 tgcgcatagt cggccagctc cggcagcggt tcgatcatcg gcgatggcag ccgatactcc
     9661 tgcgccagac gcaggaccgg tgagggtgcg tcgcgcagca ggtcgcgcac ctgatcccac
     9721 ggcagggtgc cttcgatcgg caattggcca ctgacctcat gcagttcgac caggctgcgg
     9781 atggtgacgg tcagggtggt gtggggctct tcgatcgaga agtagatcac ccggttgccg
     9841 aagtagtctt catgctcctg ataggcgtgg gggcgcgggg cgatgtcgat ggcgcgctcc
     9901 agacagtgct gccgcgcact gttcagcgga atgatgtgcg ccaggttgtg acagagcgag
     9961 acggtctcgc catacaggta atgggtgcga tgttcgacac ggtacttcat acctcctgct
    10021 ccgcgagggt gttcgacagc aactgttcgc tctgcgggtg gttgaggctg aaatagagtt
    10081 gggtgatctc gaccgagagc tggcccagcc cctgaccgag ccggtgcagc aactggtcga
    10141 gctgttgatg gccggtttca tcgggagtgg ccagcgccag ggtgtcgcag aggcgaagct
    10201 ggctgaagac ctccagcgtg atccgctcgg ccttgcttgg atggcgcggg ttgtgtccgg
    10261 gcagccgggt gaggtgctgt tcgatcagcc gcagctggta aatcagtgca cgcggattgg
    10321 tggtgtcgag cagcagcagt tccagcgcag tggccaactg tggtgcggtg cggtagcgcc
    10381 agcggtggat gatcaggctc tcctgcaccg aaagcagccg ctcgaggatc agatagcgca
    10441 cgatttcatc ctggttctgc accagcgtgg tgcgaatcag gtcgatcagg ttgatgccgc
    10501 gctccagccg gcggccgatg tcgagcagca tccagccatg ctcgtgggtc atgctttcgg
    10561 tggtctggcc ggtgagcgcc agcagcaccg tgaccagccg gtcgagttgc gcctgcagcc
    10621 gtcccagttc gtgtgccgga tggttctgca tcgactcgag catcttttcg atctcgtcga
    10681 tcacccgcca ggtgtcgctg gtccagagat cgcgcaggtt gaaggcggcc tgcaccagtg
    10741 cccgcaatat ctgaccgatg ccgccgaccc gctcctcgtc gagtaccagc gagaagatct
    10801 cctcctgtgg tgccagcagc cgcgcctgac tctcttcgcc gacgaagccg ggatgggtgt
    10861 gggtcacctc ggtcagtgcg cgcagcagtt gatagaggca ttcgcggtcc gactggtcct
    10921 ggtactcctc gatctcgtcg agcttgagcg cgacattgcg cagcagccgg atggtgaatt
    10981 cggcccgctc ggcgtaacgt ccgacccaga acaggttttc accggcgcgg ctcggcagta
    11041 ccccctggcg ttcgagtgcg ctgggatgca gtccatccgg cggcagcagg ctgacatggg
    11101 gctgcggccg gtcggccagc acccaggtgt ccttgctggt gccgccggtc tggttgctga
    11161 tctgcagcat ctcatggctg ggagcgatgc gggtgagccc acccggcatc accgcgtagc
    11221 catcaccgtc actgaccaga aaggtgcgcg tcaccacctg acagggttgc agccgaccct
    11281 ccaccagcga aggagtgctg gcgcccgcga gcggctcctg acccacatag aggtggggtt
    11341 ggcgctcgat cctgtcgcgc cagccctgta gctggctgcg gctcagttcc ggcccgaaga
    11401 tcgagccgat ccggcggttg atcggcttga tcagcaggcg gtcgaggttg tcgagcacat
    11461 ggcggcggct cttctcatcg ccgcaccacc agctgttggc cgaggggagt tgcagctcct
    11521 gatcgagcag cacccgcgcc agacgtggca gaaagggcag caggccggga ttttcgagca
    11581 gcccgctgcc gagcggattg gcgatcgcca ccttgccgcg gcgtgcggct tccagcaggc
    11641 ccggcacccc cagccgtgag ttttcgagca gctccagcgg atcgcagtag tcgtcgtcga
    11701 gccgacgcag gatgacgtcg accggtttca gtccatccag cgatttcatc cacaccgtgc
    11761 tgtcgcgcac ggtgaggtcg tcgcccacca ccaggttgta gccgaggtag gaggcgagat
    11821 aggcatgttc gaagtaggtt tcgctgtagg ggccggcggt cagcagcacc acccgcggat
    11881 cttcccgctc gcgcggtgcc aggcgcgcca gggtgttgcg cagcgtgcga aagaactggg
    11941 ccagacgccg cacctgccca tcgcgaaaag cgttgggcag gattcgcgcc agcgcgatgc
    12001 gggtctccag tgcatagccg gcgccggagg gcgcctgggc acggtcgttc atcacccaga
    12061 gctggccatc ggcgccgcgc gccagatcgg cggcatagag cagcaactgg tgcggccccg
    12121 gcaccgtaac actctgacag ggccgcagaa agccgctgtg gcgaaagacc agctcggccg
    12181 gcagcagccc ctcgcgcagc aggcggcgct cgccgtagag gtcacgcaga atgaggttga
    12241 acagctcggc tcgctgggtc agggcggttt cggtcttctg ccactcctgc tggctgatca
    12301 gcagtggaac cgggtcgaga tcccagccgc ggcgtggcgc ctccggctgg ttgtagaggt
    12361 tgtaggtgac gccattctcg cgcagcagct ggcggccctc ctgccggcgg cgctccagct
    12421 ccgcgccgcc cagacggtcg agggtgtgga tcagcggccg ccagaagtcg cggggctgac
    12481 cttcgccatc gaccgccggg tcgggcagcc acatctcgtc gtgcgctgcg gcatggtgtg
    12541 cgtaatcgga ggcgatcagg ctgaagtggc cgcggtcggc attgagcggg gagttcatcg
    12601 gccggttgtt cttgcctggg tgacgtgatg ctgcgggcgc ccgtcagccc atgctgacgg
    12661 gcctgccatc cggcgtctca ctgaaacgat gtgggacggc gcagatcgag agtatacgga
    12721 tattcccggt ttctttcctg cggtggcggc ttcatcgatt cgaccccgct gccacgcggt
    12781 tcgaagcggg agacgccgcc gaccgctggc ggcggctgca acacccccgg cgtgtggcca
    12841 aagccggcga agcgcgagac gcggcggctt tcggcctcgt aggcattgac cggaaagcgc
    12901 tcgtagctca atccgccggg gtgggagaca tggtaggtgc agccgccgat cgagcggcca
    12961 ttccagctgt cgatcagatc gaacaccagt ggcgaatgga tgccgatggt cggatgcagt
    13021 gccgaggggg gctgccaggc gcggtagcgc acgccggcga catattcgcc atgggtgccg
    13081 gtgccggcga gcggcacctg ccggccgttg caggccagga tgtagcgccc atcggtcagg
    13141 ccgctgaccc gcacctgcaa ccgctccagc gatgagtcgg tgtagcgggc ggtgccgccc
    13201 agcgccatct cctcgccgag cacatgccag ggctcgatcg ccatgcgcag ctcgatctcg
    13261 atcccctgga tcgacaccga accatagtgg ggaaagcgga actcccagaa cggctccagc
    13321 catttctgct cgaagggcaa ccgggcatcg cgcaggaact gcgccacgtc ggccaagtcg
    13381 cgccagatga agtgcggcag cagaaagcgg tcatgcagct cggtacccca gcgcaccagc
    13441 ggctgctggt aggggcgctc ccagaagcag agcagcagac tgcgcagcag cagcatctgc
    13501 accaggctca tgcgggcatg tggcggcatc tcgaagccgc ggaattcgag cagcccgagc
    13561 cgaccggtgg ccgagtcggg cgagtagagc ttgtcgatgc agaattcggc acggtgcgta
    13621 ttgccggtca gatcgaccag cagattgcgc agcagccggt cgaccagcca gggttgcggc
    13681 acctcgccgg tcggcatctg tgaaaaggcg atctccagct catagagggt ttcgtggcgc
    13741 ccctcgtcga cccgcggcgc ttggctggtc gggccgatga actgtccgga gaagaggtag
    13801 gagagccccg gatggtgctg ccagaaggtg atcagactgc gcagcaggtc gggacggcgc
    13861 agaatcggac tctcggccgg tgtgcgtccg cccagcgtga catggttgcc gccgccggtg
    13921 ccggtgtggc ggccgtcgag catgaacttt tcggtgccga ggcggctctg gtgtgcctct
    13981 tcgtagagcg cctcggtgtt ggcgaccagc tcgcgccaac tgccggccgg ctggatgttg
    14041 acctcgatga cgcccgggtc gggggtgacg gccagcttga ccaggcgcgg gtcgcgcggt
    14101 ggttcgtagc cctcgatcag caccggcaga tcgagggccc tggcggtcgc ctcgatcgtg
    14161 gcgatcagat cgaggtagtg ctccagatgg gtgatggggg gcatgaacag acagagccgg
    14221 ccgtcgcgca cctcgatgca gatcgccgtg tgcggcactt cgacccactg gggtgaacca
    14281 ttttcgtcct cttcggcatc gaccggtgcc tgcggatgaa tctccggatg caggtcgggc
    14341 ggatgctgga cgtagctgta acggcgtgcc acctcgccgt ggatgtcgcc cagttccggc
    14401 agcggttcga acaggctgcg cggatggcgc tccgtggtcc gtcgcagcgc ctgatcgacc
    14461 cagggcagtg catcgagcgg cagccgcagt cccatcggcg aatcaccagg cgccagaaac
    14521 agctggccac ggcgaaactg ccagggaccg ctgcgccagc attgtcgacg ccagtcccac
    14581 tgaagcggca gggcatagcc ggtcggctct cccaggccac ggtcgagcag cgccagcagc
    14641 cggcggcgtt cgtgttcgtc atcgagcgcg gccttggcag ggtcgaggtt gatcggcaga
    14701 ttgccttcgc gccagaggta ataggcggtg tcctcgaagg cgggcagcag atggcggccg
    14761 tcgagctgca gccgctggca cagcgcttcg gcaaaggtcc gggcatcgtg gatgccgtgc
    14821 tgtcccgcgc tggccggatc ggccagcagc cgtggctcat gccagaccgg taccccgtcg
    14881 ctgcgccaga aacagttgta ggcccagcgc ggcaacggct cgccgggata ccacttgccc
    14941 tgaccgtagt ggcgcaggct gccggtggcg aagcgcgtcg acagccgatc gagcaggttt
    15001 ctggccagca cccgcttgtg gctgccatcg gcggcgatgt tccactcggc cccctccatg
    15061 tcgtcgatcg agacgaaggt cggctcgccg cccatggtca gctgcacccc ctgctgttgc
    15121 agccgctgct ccacctcgtc accgagctga tcgatgctgc accactcctc ttcgctgtag
    15181 ggcctggtga cgcggggatc ctcatggatg cgggtcaccc ggttgaggaa gctgaactcc
    15241 gtctcgcact catcgctgta gccgaagacc ggtgccgcgc tttcgggcga ggcggtgcag
    15301 gcgagcggga tgtgcccctc ggcggcgagc agcccggagg tcgggtcgag gccgatccag
    15361 cccgcgccgg ggatgtagac ctcgcaccag gcgtgcaggt cagtgaaatc ctgctcgggc
    15421 cccgatggcc cgtcgagcga cttgacgtcg gccgtcagct gcaccagata gccggagacg
    15481 aagcgggcag ccagaccgag gtggcgcagt atctgcacca gcagccagcc ggagtcgcgg
    15541 caggagccca gccgcttctc cagtgtctcg tcgcagcgct ggatgccggg ctccatgcgc
    15601 agcgtgtagt tgatgtcgcg gtagagccgc tggttgagat cgaccagaaa gtcggtggtc
    15661 cggcatggtt cgcggccgat ctgcccgacc caatgcatca gcagtggccc cgactcgttg
    15721 atctcgagat agggcgccag ctccttcttc aaggccggcg ggtagtcgaa cgggaagctg
    15781 ttggcgtact cgtcatagaa aaaatcgaac ggattgatca cctgcagccg ggcgatcacc
    15841 tccaccctga catcgagctc gcgcaccttc tccggaaaga ccacccgcgc caggtggttg
    15901 ccgaacggat cctgctgcca attgatgaag tgctcggcgg gcccgatctt cagcgaatag
    15961 ccctcgatcg gtgtgcggct gtgcaccgcc ggccgcagcc ggaagatgtg cggggagagc
    16021 tgcaccggcc ggtcaaagcg gtagtgggtg tggtgttcga ttgcgacacg gatcgacatg
    16081 atgggctccc ctttctctcc agctctgtga tgatgcgtct gccatgaagc agatggcaga
    16141 tccatgcgtt gcgcagtgca gtcaaagcaa taagcgtgcg cacccgtcag aatgtcgagg
    16201 aaaacatgcg gaggtcagat tgacaatgaa actcaggcat gtgcattctc gccgacaggt
    16261 tgatgctgaa ccgtttttga tcataacttt ttgaaggaga acaacccatg aaggcgtttc
    16321 gtgccgttcc gctcctgctg ctgacggcgg cttttgccat cgatgtccat gcccagtgtg
    16381 cggcgagcac ccgagtggcc cagcccgagc gggtgctgac ccaaaagctg gtctgcgctc
    16441 acaagggggc gcccgacaag ccgggagatc gctggtcgga gctgcacacc aggaatggca
    16501 gcggtggcgg caccccgctg atcgaatggg cacgcggtgc acaagatccg gtggacccaa
    16561 gccgcaatgt tggaacctgg aaggtgcagg gcagcaacat cgtctacagc tacaccggcg
    16621 atgggtcata cacctaccag cttttcaacg atggcgctgg ccaatattcg ttgtgtgaca
    16681 gtcagggcgg tacggtcaag gccacgatca tgaagctggt tccgtcgcca tcgggcacca
    16741 acccctgcgg ttggagcaat tgagtggaat cgattcccct caagggttca tgctgatcgc
    16801 gcgccacccg cgtgtcggta aagagtgatc tgcgcggggg cggagggccg ggcgttcctc
    16861 tgtgcggcgc gcggcccgtt cttttgtctc tttcagcgcg gacgatcgcc gaatgacgct
    16921 cttcaatctc cggcagcgcg actttttacg gcggctcgaa ctgccactgt ggcaggcgcg
    16981 gcagccgctg ccgggtgccc gtcagacccg ggtcgagccg ccagcgccgc ccgaagcgtc
    17041 gccctcggtg accttgccat cgccgctggt ggatgcactg ccgacggcgt ccctggctga
    17101 tcaggctgcg actgccactg aatttgcact ctatcatcga cgcctgcccc gtggctggct
    17161 gctgctgtgc gacctcgaag cgcccgcgca gcagcttgca gcggggccac gccggctgct
    17221 cgacaacatc ctgttcgcgc tcgacgttga ggcggatgca ccggccgccg agggggtgct
    17281 gcgctggccg cccccattgc cgggactcca gctcgacgtc gatcctgcgg cggccgccca
    17341 ctttcttgaa gccttcatcg ccggccagca ggagcgcgaa ggggtgacgc atctgctctg
    17401 ttttggtgca aagccgctgc agctgctgag gctggccggc gtggcggcat cggtcgaact
    17461 gggaacgcag cccggtcagt gcagcgcggg cggaatgacg ctggtggcga cccatgcact
    17521 tttcgaactg ctggccgatc cgccgcgcaa ggccgccagt tggcgccatc tgcggccact
    17581 gctgcagccg gggagcagtc gcggtgacga ccgcccatcg cattgatccg atcgagatcg
    17641 cgccgctgct gtggtccgat ctggtcgagg tggtggccat cgacgccgcc agtgatcccg
    17701 ccccttggtc gctggcgctg ttcgagcaat cgttgcaggt gggccatctg ggtcacaagg
    17761 cgctctgcgc cgggcagatg gtgggctaca gcctgacgca gctggtgcag gatgaagccg
    17821 agctgctgcg cattgccgtg cttgccgatt ttcgccgcgc tggccttggc cgggcgctgt
    17881 tgagtgaggt catcgccgtt gccatcgacg ccggctgtcg gcggctgctg ctcgaggtgc
    17941 gcagcggcaa tcaggcagcg atcgcgctgt atcaacaggt cggttttcgg acggttgggc
    18001 agcgacgcgg ctactacccg gcactgccgg gcggccagaa gcgtgaggat gcactgctga
    18061 tggcgctcga ctgcgctccc cggcaatgag ccacccgcct gcgcgctggc gcatcggtgt
    18121 cagccgctgt ctgctgggtg agccggtgcg ttatgacggt ggacacaagc gcaacggcta
    18181 cgtcgtcgag accttgcaac gccatttcga tctggttgcc ttctgtcccg agctggaagt
    18241 cggtttggga gtgccgcggc cgccgatccg gctggtccgg cacgcgcaga gtgtacgggc
    18301 gttgggggtg gagcagcccg aactcgacgt cacgctgccg ttgacccgct acctcgacca
    18361 agtgcgcgag cgtgtggcgc cgctggtggg tttcatcctc aagcagggat caccgagttg
    18421 cggcctggag gtcgcactgc atgaggcaga gcgaccgatc ggcaccaccg ccggactctt
    18481 ttccggcaga ctgcgggcgg ccttgccgtg gttgccggtc gaagaggagg ggcgactgag
    18541 cgatccgcgg ctgcgcgaga actttctgac ccggctcttc gttcacgggc gctggcgtga
    18601 tctgcccgat ggcggcctcg acttcgccac cctgctggcg tttcaccgca gccacaagat
    18661 gctgctgctg gcgcacgatg aggcgtgcta ccgccgcctg ggacgctggc tcgcgaccgc
    18721 gcggcagctc gaacccgggc agcgggccca acagtacctc ggccaactgc tgcaagggct
    18781 cgaacggctg ccgacgcccg gttcgcatgg caacgcgttg cagcatctgg ccggctttct
    18841 caagcggcag ctcgatgcgc ggcagcgcgg cgaactggcc gccaccatcg acgcctaccg
    18901 tcagggggca ttgccgtgga ccacgccggt tgcgctgctc aaccaccact ttcagcacca
    18961 tcctcacccc tttgcgctgg cgcagcacta cctgcggcca ctgccggccg agctgcttgg
    19021 gccgtgaccc gcggggctgt cacgcccgtc acgccttgaa gcgtcagcgt gctgctggga
    19081 caatggccga ttcgcagtgc cgcccgcatc gaccttccgc cagcgaagcg caaagtgggt
    19141 gtcgattcgc ggctgaaaag tcaaaaatct tctggatcaa aaggttaatc cagccatgct
    19201 gctgatgatc gacaactatg actccttcac ctacaacgtc gtgcagtatc tcggcgagtt
    19261 gggtgccgag gtggtcgtgg tgcgcaatga cgagatcgac atggcgggaa tcgagcggct
    19321 ggcgccctcc catctggtga tctcgcccgg tccttgcacg ccgaacgagg cgggcatctc
    19381 ggtgccggcg attcgccact ttgccggtcg cctgccgatt ctcggcatct gtctcggcca
    19441 tcagggcatc ggtcaggcct ttggtggccg ggtggtgcgt gccggtcggg tgatgcatgg
    19501 caagacgtcg ctggtgcatc accgtggtca gggggtgttt gccgggctgc ccaatccgtt
    19561 cgtggccacc cgctaccact cactggtgat cgagcagtcg tcattgccgt cggtgctcga
    19621 agtgaccgcc tggaccgagg atgagtcggg gcggatcgag gagatcatgg gggtgcgcca
    19681 ccgtgagttc agcgtcgagg gggtgcagtt tcatcccgag tcgatcctga ccgaaggcgg
    19741 acatgcgctg ctgcgcaact ttcttgccca gcagggcggg cggcggcagt gagcagcccg
    19801 aacggttcga tgcccaccga catgaccttg ccgcaggcca ttgcccggct ggtcgaacgg
    19861 catgatctca ctgcggtcga gatggcgcag gtgatgcggc tgatcatgac cggacaggcg
    19921 agcgccgcgc agatcggcgc gattctggtg gcgctgcgca tgaaagggga gacggtcgat
    19981 gagattgccg ccgcggcacg ggtgatgcgc gagctggtga cgccggtgcc gattcgcgcc
    20041 gcgaacgcgg tcgacacctg cggcaccggc ggcgatgccg ccggcctgtt caatgtctcg
    20101 accgccgccg cgctggtggt ggccgccgcc ggtggcaagg tggccaagca tggcaaccgt
    20161 tcggtgtcga gcaaaagcgg cagcgccgac ctgctcgaag cggcgggggt gcggctcgat
    20221 ctctctgcgg tgcaggtggc acgctgtgtc gatgaggtgg gggtgggttt catgttcgcg
    20281 ccggtccacc atggcgcgat gaagcatgcc atcggcccgc ggcgcgagct ggcgattcgc
    20341 acgctgttca atctgctggg tccgctcacc aacccggccg gcgtgcgccg gcaggtgctg
    20401 ggtgtcttcg atcgccactg gctgcggccg ctggccgagg tgttgcgcga tctgggcagc
    20461 gaccatgcgc tggtggttca cgccgaggat gggctcgatg agttctcgct ggccgcgccc
    20521 acgctggtgg ccgagctgcg cgatggtgaa gtgatcgaat accgggtcac cccgcagcag
    20581 gtgggtttgc atggcgccgc gcaggatggg ctgcgggtcg gcaatgccga agagagtctg
    20641 gcgctgatcc gcgctgcgtt ggccggcaag gccgcgctgg cggcagagct gatcgccttc
    20701 aatgccggtg cggcgctcta tgtggccggg gtggcgcgcg atctgaaaga gggggtgatg
    20761 atggccgatg acaccattgc cacggggctg gcgctggaga agctggcgga gctggtcgag
    20821 gtgacccgct gcatggagac gaatgcatga gtgcgccgac cattctcgac aagattctgg
    20881 cgcgcaaggt cgaggaggtg gcgcagcggc gccagcaggt ttcattcggc gaactggagc
    20941 gtctggccag gacagccccg gcgctgcgcg gttttgccgc ggcgatcgag cagaagatcg
    21001 acgccggcag cgcggcggtc attgccgaga tcaagaaggc gtcgccgagc aaggggttga
    21061 ttcgcgccga cttcgacccg ccggcgctgg cccgcagcta cgaggccggc ggcgccgccg
    21121 cgctgtcggt gctgaccgac cgcgatttct ttcagggtgc cgatgagtat ctgcaacagg
    21181 cgcgcgcggc ctgcgcgttg ccggtgctgc gcaaggattt tctgatcgac ccctatcagg
    21241 tggtggagtc ccgggttctg ggggccgact gcatcctgct gatcgtggcg gcgctcgatc
    21301 agtcacgact gctcgaactg gccgatgtgg ctcgtgagca ggggctcgac atcctggtcg
    21361 aggtgcatga ccgcgccgaa ctggagcggg cactgcaact cgacacatcg ctgatcggca
    21421 tcaacaaccg tgacctgcac agcttccaga cccggctcga aaccacctgg gcgctgctgc
    21481 cgctgattcc ggccgggcgc cgtgtcgtca ccgagagtgg catccacacc gacgcggatg
    21541 tggctgcgat gcgtgcgcac ggcgtggcgg cctttctggt gggcgagtcg ctgatgcgcg
    21601 ccgatgatcc gggcgcgcgg ctggcgcagc tctttgggcc gctccggctg gtctcgcctt
    21661 gagttcagct ggggattgcg gcagaatcgc ccgcttcgcc gtggttgcgg atgggaatcc
    21721 ggggtcgtcg gtgaagtgcc atggtggtcc ctaccggtcc cctcgcactg ctaggctgcg
    21781 aaccccgcca ggcccggaag ggagcaacgg tagtggctga tgcaggtgcc ggggtgtggc
    21841 tggtaggggc tgcctcccga tctgcggcgc aggctgcatc gcgccgccgg ccatccccgc
    21901 cgggtcttcc cgaatttcaa tcagctgttg cgtcaacatg agctatcagg tcctggcgcg
    21961 taaatggcgc ccgcgcaact tcgagcagac ggtgggacag cagagcgtgc tgcgcgcgct
    22021 ggcccatgcg ctcgaccatg accgtctgca ccacgcctac ctcttcaccg gctcgcgcgg
    22081 cgtcggcaag acctcgattg cccgcattct ggccaaatgt ctcaactgcg agcagggggt
    22141 ctcctccact ccttgcgacc agtgcgccag ttgcgtggcg atcaacgaag ggcggttcat
    22201 cgatctgatc gaggtcgatg cggcttcgcg caccaaggtc gaggacaccc gcgagctgct
    22261 ggagaacgtg cagtacacac cgtcactggg acgcttcaag atctacctga tcgacgaggt
    22321 gcacatgctc tccggccaca gcttcaatgc gctgctgaag acgctggaag agccgccggc
    22381 ccatgtcaag ttcctgtttg ccaccaccga cccgaagaag ctgccgctga cggtcctgtc
    22441 gcgctgcctg caactgcatc tgaaggacat tccgccggca ctgatcgcgg agcatctgca
    22501 ccgggtgctc gacctcgaag gcatcgcaca tgacgacggg tcgctgaaga tgctgggacg
    22561 ggcagccaat ggcagcctgc gtgacgcgct gagcctcacc gatcaggcca ttgcctttgg
    22621 cggtggccag ttgaatgagg cagacgtgcg gcagatgctg ggccatgtcg atcagcagtc
    22681 ggtgctgcaa ctgctgcagg cggtggttgg cggcaatgcc gtgcaactgc tggatgaagt
    22741 cgagcagctc gctggccatg ggccggacta tcggctggtg ctcgatgagc tgatcgggct
    22801 gctgcatcaa ctgacgctgg cgcaggtggc gccgggcacg ctccatggcc actggtggga
    22861 tggtgaggcg gtcacggccc tggcggcgca gatggccgcc gaggagcttc agctctatta
    22921 ccagattgcc ctgcatggcc ggcgcgacct gccgctggca ccggaaccgc gcagcggctt
    22981 cgagatggtg ctgctgcgga tgctggcctt ccgccccgac gacggcacgc ctgacagcgc
    23041 ggcggaaaaa aaaaagcccc tgattgagcc gcagcgctcg gccacggcgc tgctcgatgt
    23101 gccgggcagc cccgcaccat ccagcagacc catcgcacct gcggcaattg ctccggctcc
    23161 accaccgccg cccgccgcgg tcacgccacc cgcgtcacag ccagaggcca ctgcgctcgg
    23221 tgaacagtgg atcgggctgt gtggcaagct cgacctgagc gggatgagtg ccaacctggc
    23281 cgcccatgcc gagctggtcg cgcagacggc gcagggttgg cgactgcgca tcgatccggc
    23341 gcactcggcc ctgctcgatg ggccccatgt cgggcgcatc ggtgaggcgc tgtcgcgcca
    23401 cctgggtcaa tcggtcaagg tggagttcga gctgggtccg attcagggcg aaaccccgct
    23461 gcgctcgcgt gaacggcagc agcgcgaagc acgcgagcgg gccatcgccc ggctggaagc
    23521 cgatccgaag gtcaaggccc tgctggcccg cttcgaggga tcgagcctcg accatgagtc
    23581 gatcgaggtg ggctgactct cacagcccaa gtggttgtag ccagcgcgtg gcgatgccga
    23641 ccggacgcgg caggctataa tggcccgcct cactttcttg atctcttcag gagcaaccga
    23701 tgaaaccggg cattggcgat ttgatgaagc aggcgcagaa gcttcaggag cagatgcaga
    23761 agacccagga tgagctggcc aatctgcagg tgtgcggtga gtcgggggcc ggattggtga
    23821 aggtgaccat gaccgggcgc cacgacgtga agcgggtcga gatcgaccct gcgctgctgc
    23881 aggaagagaa agagatgctg gaggatctgc tggctgccgc ggtcaatgat gccgtgcgtc
    23941 gggtcgagga ggccagccgc gacaagatca gcagcctgtc ggcggggctg aatcttcccg
    24001 gcggcttcaa gatgccgttc tgaggcggac tgacagcacg ccgatgagtg aactgatcaa
    24061 tcagcttgcc agcgcgctgc gttgtctgcc cggtgtcggg cagaaatctt cgcagcgcat
    24121 ggtgatgcac ctgctcgagc gcaaccgctc cggcgggctg cggctggccg aggccttgat
    24181 tcgctgcctc aatgaggtgg gccactgcac gcgctgccgc aacttcaccg agcagccgct
    24241 ctgcgcggtc tgtgccgatt cccggcgcga tcagagcctg ctctgcatcg tcgaaacccc
    24301 ggccgacatg ttggcgctcg aacagtcggg cggttatcgc gggctctatt tcgtgctgat
    24361 gggtcgcctc tcgccgctcg atggggtggg tgccgacgag ttggcactcg atctgctgtg
    24421 gcggcggctg gccgaagagc cgatctgcga ggtgattctg gccaccagtg ccaccatcga
    24481 gggcgaggcg acctcgcatt tcattgccga aggcttgcgt cccgacatca gtgtgacccg
    24541 catcgcgcag ggggtgccgc tcggtgggga gctcgaatat gtcgatgccg gcaccctggc
    24601 gcgcgcgctg agcggaagaa aacagtttga acgcttctga gccgcccttc gtcgaagtga
    24661 gccacgatgc cgaattggcg cagtggtgcg agcggctggc cggcaccgcc gtgctggcgc
    24721 tcgacaccga gttcatccgg gtgcgcacct accatccgca gccggcgctg ttgcagctct
    24781 cggatggccg gcaccatctg ttgatcgatc ccttgacgct gagccgctgg cagccgctgg
    24841 ccgactgcct gaccgatccg cagcaactca aggtgttcca cgctgccggt gaggatctcg
    24901 aagtgtgccg gcggctgttc gacgcggtgc cgcgtccgct gttcgacacc caggtggcgg
    24961 cggccttcgt cggtctcggt ttcagcatcg gttaccagcg gctggtcgag caactgctcg
    25021 gcgtcaccct cgaaaagggc gaaagccgct cagactggct gaaccggcca ctgaccccgt
    25081 cgcagaagca ctatgccgcg caggatgtga tccatctgca tgcattgcac cagtggctgc
    25141 atcagcggtt gcagcagcgt ggccatttgc gctggttcga gctggagatg gagcgcacgc
    25201 tgcaagaggc cgagcggccg atcctgccgc aggagcgtta tctgcatctg cgcgcggccc
    25261 agccgctctc atcggccgag ctggcgattg cccggtcgct ggcggcatgg cgagaggtcg
    25321 aggcgatccg gcgtgacctg ccgcgtggat tcgtgctgcc cgaccgggcg ctgctcgacc
    25381 tcgttcgccg tgcgccgaac gatctctctg gtctgagtgg cattgcggag ctgcaccctt
    25441 cggtgatccg ccaccatggt cagaccctgc tgcaactgat cgcagcggcc aggaccgaac
    25501 cggccgatca gcagccgcca ccgctctcga cgcagttgga cgccgggcag aaggcgcgcc
    25561 tgcagcgcct caaggagcag gtgcgggggc gggcccagca gctcgacatc gcggtggagc
    25621 tgctggccag cagccgcgag ctgagcgact gggtcgtgag cagcagtgac gcgggggcag
    25681 cgcagccgcc gcggcggctg ctggaagggt ggcgcttcga agcgattggc gatcttctga
    25741 ccacggagtg aggtgacaga tgggtgaaca tcaaccggca acgccgatcc tggtcgaggt
    25801 gtttcgcagc cacaagcgcg aaggggccta cctctatctg acccggggca agggcttcgg
    25861 ccatctgccg gcggcgctgc agggctggtt caggtcgacc gacctggcca tgacgctgtg
    25921 gctgaaaccc gataaaaagc tggcgcgggt cgacgcggcc gaggtgctgc gcgccctcga
    25981 cgagcagggt ttctacctgc agatgccgcc gccgcgcgag cctgatccac ttgctccgcg
    26041 ataaagaaca cggttcaggc gggatcgcag cactgaccag tcgaaacagc atgggaggtt
    26101 tacagccggt caccgattgc ctggcctctt tgcgctttat aattccggac acatgactga
    26161 atcgaggtgg cttgaacatg cagaatccgc ggatcaaacc gctgccggcg ctgatcttct
    26221 cttcacgctg gctgcaattg ccgctctact tcggcctgat tgccgcacag ggcgtctatg
    26281 tggtgctgtt cttcaaggag ctctggcact tggtgcatgg ggctctggaa ctcaacgaac
    26341 agcagatcat gctgatcgtg ctgggtctga tcgatgtggt gatgatctcc aacctgctga
    26401 tcatggtgat cgtcggcggc tatgagacct tcgtgtcgcg gctgggcatc gaaggacacc
    26461 cggaccagcc ggagtggctc tcgcacgtca atgccaccgt gctcaaggtg aagctggcgc
    26521 tggccatcat cggcatctct tcgatccacc tgctcaagag cttcatcgag gccggcatga
    26581 tcggcgtatt gccgcagtgc agcagtgttg ccgccggcgt caagtgcgcc accatcaccg
    26641 agaacggcat catgtggcag accatcatcc atctggcctt cattctctct gcgctcggca
    26701 tcgcctggac cgatcgcatc atgagtggtg ctgtgtcgca acacgctgag aaatcgctgt
    26761 cggaacattg agcccggtgg ttgcgctcag cccacgactc aactgttcag atgcagtgcg
    26821 gcattgagtt cgatgctgcg ccggttggtg cggcattcga tggcgccgct ctgtgaattg
    26881 cgcaggaaca gcagatccga ctgaccggcc aactcccgcg ccttgatcag ccgctgttcg
    26941 ccatcggcgc tgatcagcag caccttgctg ccggcggtga ggtagaggcc ggcctcgatg
    27001 gtgcagcggt cacccagtgg aatgccaagg ccggcattgg cgccgatcag gcacttctcg
    27061 cccaccgaga tgacgacgct gttgccgccc gacagggtgc ccatggtgct gcaaccgccg
    27121 cccaggtcac tgcccttgcc gacggtgacc ccggccgaga ttctgccctc caccatgctc
    27181 tcgcccagcg tgccggcatt gaagttgatg aagccctcgt gcatgaccgt ggtgccctcg
    27241 cccaggtagg cgccgagccg cacccgggag gcatcggcga tgcgcacccc cttgggcacc
    27301 acgtaatcgc tcatccgcgg gaacttgtcg actgcatcga ccaccagcgt gcgccccgcg
    27361 atgcgggcgg cgagctggcg ttgcggcagc tcgctgaccg cgatggcccc ctcgctgctc
    27421 caggcgacat tgggcagcac gccgaagagc ccctgcaggt tgatgccgtg cggcttgacc
    27481 agtcggtgcg acagcagatg cagcttcagg taggcctcgg gcacgcaggt cggtgggctg
    27541 tcttcggcca gcagcatcac gcagccgggg cggtggctgg cggcgagctg gtccgccagt
    27601 cgtgccgact catcctgctg ctgttgactc agtgccgtgg ccagggcacg ccagccggcg
    27661 gcatccatgg cgatcagccg gttgtcgctg gcctggccca gcgtgctgtt cagggcctcg
    27721 accagtcccg gtgccgggtg gagcagcggg gccgggtagt agacttcgag ccagttgccg
    27781 ctgctgtcct gggtgccgat gccaaagccg agggcaaaat gtgcggaatt catcgagggc
    27841 tccttgaggt ggaatcaggt ctgacagggg agtgaaaggg agcgggcgtg cggtggtcag
    27901 cgcaactgcc gctgatagag atcgggatcg aagccgagca gcaggccgcc cgaccactcc
    27961 agcagcgggc gtttcatcag cgtcgggttc cccagcagca gctcgatcgt gcggtcggca
    28021 tcgacatcga tgcgcagcga ctcaggcagc tgtcgccaac tgctgctctt gcggttcagc
    28081 agttccggtc cgagccgttc cagccagaca accagttcgg tgcgctgcgg ggcgtcctgc
    28141 cgcaggtcac ggaactggtg ggcaacgcca tggtcacgca gccagcgcag cgcggcgcgg
    28201 cagctgtcgc agctcttgat gccatgcagg accacatcgg taggcagttg catcaaccca
    28261 acccctcgac gaagcggcgc agccgctctg ccccctcgat gcactcatcg agttcggcca
    28321 ccagcgccag acggacgtgg caggcgccgg ggttgtcgcc attcacatcg cgggagagaa
    28381 agctgcccgg caccaccgtc agcttctgct gacgatagag ctcgcgggtg aactgctggt
    28441 catcgagcgg caccctgggc cagaggtaga aaccgccgcc cggccggacg acatcgagcg
    28501 atcctctcag aatgtcgagc accgccgcga atttctgccg gtaccgggcg cggttttcga
    28561 ccacatgggc ctcatcctgc caggcagcga tgctggcgcg ctgcaccggc accggcagtg
    28621 ccgcgccatg gtaggtgcgg tagagccggt agcgctcgat caggcgcgca tccccggcga
    28681 cgaagccgga gcgcagacct gccaggttcg agcgcttgga gagcgaatga aaggcgatga
    28741 cccgtgcgta gtcggtcagt cccagccggg ccgccgcctg caggatgccc ggtggcggag
    28801 ctgcctcgtc gaaatagagc tcgctgtagc actcgtctgc cgcgatcacg aaatcgtgcc
    28861 gttcagccag tgtgatcagg tggcagaggt agtcgaggct ggccacggcg ccggtggggt
    28921 tgtcgggcga gcagaggtag aagagctggc agcggttcca gctggcggca tcgacctgat
    28981 cgagatcggg caggccgccg gttgcggagg agctggccag gtaacgcggg gtggcgccgg
    29041 ccagcagcgt ggccccttcg tagatctggt agcaggggtt gggcatcagc accagcgggt
    29101 tggatcgttc gctgttgatg actgcctggg cgaacgagaa cagcgcttcg cgggtgccgg
    29161 ccaccggcag cacatgccgt tcggcggtga ggctgccggc cggcaggtcg aagcggcggc
    29221 cggcccaggc ggcgatcgcc tcgcgcagtt cgacaatgcc gttgatcggt gggtagagcc
    29281 ccagctcatc cagatgctgg cgcactgttt cgagcacgaa gggtggtgcc ggatgtttgg
    29341 gctcgccgat cgaccaggag atcggtttca gcgcggcggg cggggtgact ccgtcgagca
    29401 gcgcgcgcag cttttcgaat ggataggggt gaagcagatc gagtcgtgga ttcatcgcgg
    29461 ggtccggggg cggctgacga agtgggtcgg tccggtgcgc ggccggctag ccctgtgact
    29521 gggcatcgag ctgagcgcag agtgtctgct tgagccgttg acagatgacc gggtcggcaa
    29581 tcggctggcg gttcttgtcg gtgatgaaaa agacatcatc gatgcgctcg cccagtgtgg
    29641 cgatcttcgc cttcagcagg tagatgtcga actccatgaa gatctgcccg atgcgcgcca
    29701 gcaggccggg gcggtcggcg gtgatcactt cgagcacggt gtggttcttg atggcgtcgg
    29761 tgtagagcag cacctcggtc ggaatcggga actgcttcaa ctggcgacgc acatggcgac
    29821 gcaccacatg cgggtagagg gtcggatcgg ccagatcctg cgtcagccgc tgcgtgatct
    29881 gctgaagcag tcgtcggtcg ttgatcagcg gccgtccctc ttcatccagc acgtaatagg
    29941 tgttgagcgc catgtcgttg tcggaggtga tgatgcgcgc atcctggatg ctgagcgaga
    30001 gctgctccat ggcggcggcg atggcggcga acaggttggc ctggttgcgg gtgtagatga
    30061 agagctgggt ggcctcgtcg aactggcgac tcggggtctc cttgatcagc accagtgggc
    30121 gatcgagggt ggcgtgggca tggatcgcgg ccgtgtgcca ggcaatgtcg gcagcacttt
    30181 cgcgcagaaa atagtcctcg ccgaggttgc gccagatctc ctgcgccgcg ctgagtgaca
    30241 cttctgcctg ctgcagcagc tgcagcgcct cactctgggt ctcggtgatg cgctcctgct
    30301 tgtccaccat gttttccaga ccacgccgca gtgcacggcg ggtctcggtg tagagctggc
    30361 gcatcaggct ggcgcgccaa ctgttccaca gcacgtggtt ggtggcgttg atgtcggcca
    30421 ccgtgagcag atagaggtag tcgagccgct gcaggtcgcg gacctggctg gcaaaatggt
    30481 ggatggtctc cgggtcggaa atgtcgcaac gctgggcggt catcgacatc agcaggtgcg
    30541 actgtaccag ccagaccacc aggttggtgt cacgctggct caggccatgg cgctgacaga
    30601 aggcctcggc atcgctggcg ccgagggtgg agtggtcgcc gccgcgtccc ttggcgatgt
    30661 cgtggtagag cccggcgata tagagcagct cgtacttctc gatgttgggc acgatcatcg
    30721 agaccagtgc gtactgtgct tcggtgctgc cgttcttgaa gcggcgcagg ttgcggatca
    30781 gcagcagcgt gtgggcgtcg acggtgtaga tgtgaaacag gtcgtgctgc atctggccga
    30841 tgacgcgacc gaactccggc aggtagcggc cgagaatgcc gtaacgctgc atccgttgca
    30901 gttgggtgac caggaagtgt ggcgagcgca gcaactccat gaacagggcc gtgacctgcg
    30961 gatcgttgcg cagggcctca tcgatcagat ggctgtggcg gcggatcaga cgaatggtgg
    31021 tggcgcgcac gccctggatg tcggggcggt tgccgagaat gacgaagatc tccagcagag
    31081 tgcgcggttc ctcctggaac agcgtcgagc ggctcacctc gatgaagttg ttgcgcagcc
    31141 gaaaccgctc gttcagcgtc tcgatttcga tgggttgatg gtcgatctgt cgcagtacgc
    31201 cctcttcgat gtgttgcagc agaatctcgt tgaaatcgct gacgttttgc gcagttcgat
    31261 agtactcctg catgaagttc tcgaccgaca gcttgttcgg atcatcatga aagccaaaca
    31321 gctcggccag cgtgcgctgg tgctcgaaca gcagccgctc ctcggcgcga cgggccagca
    31381 tgtgcagccc ccaacggatg cgccacagca ttcgccgtgc cgcttcgagc tggttgaatt
    31441 catcgggtga caacaacccc agtgcaatca gatcctgatc ctgccgtgcg cccagcagcc
    31501 gcttggcgat ccagtggatg gtctgaagat cgcgcagacc gcccggcgag cccttgaggt
    31561 tgggctcgag gttgaattcg gtgtcgtagt acttttcgtg acgacgctgc tgttcggtga
    31621 acttggcacg aaagaactcc gccggcggcc acatctgatc aggaccgagc gcgacatgca
    31681 ggcgcagcat cagctccggt tcaccatcga gcacccgtga ttcgatcagg ttggtcagca
    31741 ccgtgacatc gagccgtgcc tgctgcacgc actcattgag gctgcgcacg ctgtaaccga
    31801 cgttgagctt gatgtcgaac agcagggtca cgaagtgggt gatggctgac tgaagctgtt
    31861 gctgatcctc ctgttccagc aggatcagca ggtcgatgtc ggagtgggga tgcagttgcg
    31921 cccggccata gccgcccacc gccaccagca acagggcgcc ttcatcggcg ctggcctgca
    31981 gacccgaggc ccgccaggcg atcaggatca gccgatcgat gaagtgggcg cggaccgaca
    32041 ccaggtggtc gatgtcgaac tgctgccgga aggcttcgtg cagcagggcg tcagcatcat
    32101 cgagggcggt gcgcagcgct ttgatcaggt tgggtgactc gggcgtcagc gcgtcgagca
    32161 gttgatcgag ctgctgttcg agtcggctgg cagccaacgg agcgttcatc gaggtctgct
    32221 cattcgatca cttcgccctg acgcagcgtc agtacctcgc agccatcttc cgtcaacagc
    32281 agcgtgtgtt cccactgggc gctgtatttt cgatccttgg tgatcaccgt ccactggtcg
    32341 ggcagcaggg tgacatggcg cttgccggca ttgagcatcg gctcgatggt gaaggtcatg
    32401 cccggctcca gcgccatgcc ggtgttgggg gtgccatagt ggagcacctg cggctcttca
    32461 tgaaagatct tgccgatgcc atggccgcag tactcccgca ccaccgaaaa gtggttcgac
    32521 tcggcgtggg tctggatcac atgaccgatg tcgcccagcc gcacgcccgg acgcaacagg
    32581 ccgatggcac gatagaggca ctcctgggcc acctgtacca gccgccggtt caatatcgag
    32641 ggttcgccga tgaagaacat catgctggtg tcgccgtggt agccatcctt gatcacggtg
    32701 atgtcgaggt tgatgcagtc gccgtttttc aggatcttgt cgtcgctcgg gatgccatgg
    32761 cagaccacat ggttcaccga ggtgcagatc gacttgggaa aaccgttgta gttcagcggt
    32821 gccgggatcg ccttttgcac attgacgatg tggtcatggc agatgcggtc cagctcgccg
    32881 gtgctgacgc cgggctggac atggggcgcg atcatctcca gcacttcggc ggcgaggcgc
    32941 ccggcgacgc gcatcttttc gatctcgtcg gggcgcttga tcagggtcga tttcatcagc
    33001 acgctgcggc tcgctcttgg aaggtgaaaa tgggttcagt gtaacaaaag atggcggctg
    33061 ttctggagcc cgtcattcgg cgctgggcgg ggcagcctgt tgcatggcct cgacgagggc
    33121 ggcgagttca tcctcccagc gtttgctctc tttcttggag acgccaccga ggatttcaat
    33181 ggctctgcgc aacctcgccc ggctcagatc gggaccgaga atcgccatcg cctccagcac
    33241 cgacgacgac gcggcgctgc cggcaatggc aatgaagaac ggaaacagaa agtcgcgaat
    33301 cttgtgcccc atcgcgcggc tcaactgctc gatggtgcag tggatctcct ccttgttcca
    33361 gtcgcgcagc cgttccagtt gccacaatcc gaactgcaga atcttgcgga tggcctcgat
    33421 ctccaggctc ttgtgggaaa aatggctggc atcgatgcgc ggatggccgg ccacgaagtg
    33481 gccagcgatt tcgaccacgt cgctgagcag ttcgatccgg ctcctgattt cgggcacgat
    33541 gcgtttgcag tagtcatcat caaaggccca ctgccgatag agcgcaatga agcggtcggt
    33601 gtcgtactcc tcccgcagat aaagcccgtt cagccacttc agcttgtcga tgtcgaacac
    33661 aggtccgccc agcgagaccc gcgcaagatc gaatgcctcg atcatctcgg cgaggctgaa
    33721 cttgtcgcgc ccgtccggca tcgaccagcc catcatgccg aggtagttga ccagcacctc
    33781 gggcagaaag cccatccgac gataaaagtc gatgctggtc gggttcttgc gtttgctcag
    33841 cttgctcttg tccggattgc gcagcagtgg caggtgacac atcaccggcg gctgccagcc
    33901 gaagtactgg tagagcagca gatgcttggg caccgagttg atccactctt caccgcggat
    33961 cacatgggtg atctgcatca gatggtcatc gaccacgttg gccaggtgat aggtgggcat
    34021 gccgtcgctc ttctgcagaa tctgcatgtc gacctgctgc cagtcgatct cgatcgatcc
    34081 acgcagcaga tcgtcgaact ggcagcggcc ggtacgcggc accaccatcc gcaccaccgc
    34141 cgtctcgcca gcggccaggc ggctttgcac ctgtgcctga ctcagttgca ggcagtggcc
    34201 atcgtagccc ggctgctcct gcaggacgag ctgtcgtttg cgcagttcgg tcagccgctc
    34261 ctcgctgcag aaacagtgaa aggcatggcc ctgttcgagc agttgccgca catgaagctg
    34321 atagatcagg ccgcgctcgc tctgccggta gggaccgtgc gggccgccga catctgggcc
    34381 ctcgtcccag tcgagaccaa gccagcgcag cgcgtcaaag atgcgctgct ccgaactgct
    34441 gctgctgcgc tcccgatcgg tatcctcgat tcgcagcagg aactgaccgc catgctgccg
    34501 ggcgaagcag aggttgaaca gcgcgatata ggctgtaccg acgtgggggt cgccagtcgg
    34561 agagggggca atgcgagtac gaacggtcat cgaggttcca gggtaaaaca ggatagagga
    34621 tgtatcagta aagcgaccga ttatatccca gcggctcttt tgggcagggc agcaagcggg
    34681 agcatactgt aaaatggaag tataaccggc tgattttagt tacaattaaa aacacaaggc
    34741 tgcggcaggg gttggtctgc agatgggtgg gcatccatcc atctggcgct gtttgccaat
    34801 gaacttctcc gcgcagctcc tgtcccttga acagaacccg cacattttat tttcagttcc
    34861 aagccctatg aagcaacacc ttgttccatc tcgtttttct gcgttgaatc tgacccgggc
    34921 caagcgctct ttgcacctct gtcttggcct gcttctgctg tttggcgtga atttgccgct
    34981 tggcgcatcc gcccgcgaga gctaccttgc ccagagcaac aaccggcccg tggatctggt
    35041 gggtgacatc cgcaaggtgc aggcgaacca tgaagacacc ctgctggaca ttgcgcgccg
    35101 caatggtgcg ggctactggg atatggtgcg ggccaaccct gacgtggatc cctggctacc
    35161 tggcaaagga accttggtgg tggtgccatc gcgccacatt ctgccgcgca cggcgcgtga
    35221 cgggctggtg atcaacatcc ccgaactgcg gctctaccag tactaccgtg acggccaggg
    35281 ccggcagtgg gtgcgcagct atccgatcag tgtcggacgt tcggactgga aaacgccggt
    35341 cggtacgacg cgggtcgtcg ccaagcgcga gaacccggcc tggactccca ccgaatcggt
    35401 gcgcaaggag catgcggccc gtggcgacat cctgcccaag gtggtgccgg caggtcctga
    35461 caaccccctg gggcgctatg cgatgaacct gggttttgag agctacctga tccacggcac
    35521 caaccgtccc ttcggcatcg gcatgcaggt gacccatggc tgcatgcggc tctatcccga
    35581 agacattgag gagttgttca agagcacggc tgtttcgacg ccggttcacc tggtgaatga
    35641 gccggtcaag cttggctggt ccagcaattc gctgttcctc gaggtccatc tcaatgaaga
    35701 gcagaagatg gacaggaagc tctactacag tctcatcgat cagacgctga agttgctgca
    35761 gcaaaccctt ggagacatcc cgcccggcat ggacctgagc cgtgtcaagg ctgtcgtcga
    35821 gcaggcgaac ggcctgccgc ggctcatctg gacgagaccg acgacctgat gaggctttga
    35881 atccagataa gtccgagcgt tctgtcgcgc cgccatggaa aaggcaataa aaaaggccgc
    35941 aggcatcaag cgctgcggcc ttttgaagca gggtcgatta tttggacatc gacttcttgt
    36001 acatccggct cagcttctcg tcggtcgcgt tggctttgct cagcgcttcg ttggcggttg
    36061 cctgcgcctg gttggcagcg gacagagcct ggtcagcctt gcgatccgca gcggaagcgg
    36121 tggcgctggc ttcttgggct gcagccagtg ctttctgagc gtcagcggca gcctggtcga
    36181 ttttttcgtt gctggaacag ccgccgagca gcaacaggct gccgagtgcg acggttgcaa
    36241 acgtcaagga ttttttggtg ttgctcatcg gtcatttcct tttgggatag taaaagggtt
    36301 caggttcaag acgggcggta ttctcttgaa ttcagtcgtt gaattcaata gaggttggat
    36361 ggaaaaagtg attcaggaca tattttgtgt tggagatata tcgaaggagc actttcacct
    36421 tctcttccgc ccggctttca gatagacacg gttttcgtga gctggttccc gctgttgcag
    36481 ttaaaatgcg acggcttccg tttttgatct caacaggcgc gcgacaggcc ttggagtctc
    36541 atgtcacaca gctatcgctt gattctgggt tgtccggacc gagtggggat cgtcgcggcg
    36601 gtcagccgct tcatcgccga gcgggacggc tggatcatcg aggccgatca gtactctgat
    36661 ccggccagcc accacttctt catgcgtgtc gtgattcggg ctgaatcact gaactgcccc
    36721 ctcgatcagt ttcgtcggga ttttgtcgag gtggcggccc gttatgagat gaactggcgg
    36781 ctgcacgact cggcgcagcg gcagcgggtg gcgattctgg ccagccgtga gtcccactgc
    36841 ctctatgacc tgctgcatcg ctggaaggat ggcgaactgc tgttcgatct tccctgcgtg
    36901 atcggcaatc atgaccattt ggccgaggca gtggagtggc atggtgtacc gttctaccat
    36961 gtgccgatgg aaccggcccg caagcaggcc ggttttgacc gcatccatct gctgttgcag
    37021 cagcatcggg tggatgtggt ggtgctggcg cgcttcatgc agattctgcc gaacgtgctg
    37081 tgtcagcact atgccgggca gatcatcaac attcatcaca gttttctgcc ttcgttcatt
    37141 ggcgccaagc cttaccatca ggcagccgag cgtggcgtca agctggtcgg cgccacctgc
    37201 cactatgtca ccgaggcgct cgatgccggt ccgatcatcg agcaggatgt ggtgcgggtt
    37261 tcgcaccacg acaccatcga agacatgatc cgcttggggc gcgatgtaga gaagcaggtt
    37321 ctggcgcgtg gactgcgtta tcacctggaa gaccgggcgc tgatccacgg caacaagacg
    37381 gtgattttcg cctgatccac cccacccgcc gaggagaggc ggtccggagc gctgtcactc
    37441 cagtggcagc tcggttctgt ccaccacgct gcgcaataca aagctggagt gaacaccgct
    37501 tacgccatcg atgcgcgtga tccggttcag cagcagcgtc tggtaggcat ccatgtcgtg
    37561 caccagcacc ttgagttgat agtccgcctc ctgtccggtg atcagcaggc actccacgac
    37621 atgcggtatt tctctgattt tgctttcaaa attctcaaaa cgttctggag tgtgacgatc
    37681 catcgaaatg tggatcagcg ccatcagcgt gagtccgagc cgcttggcgt cgagcagggc
    37741 gcgatagccg ctgatgatgc cgttctgttc gaggctgcgc acccggcgca ggcaagggga
    37801 gggcgagagt cccacctgat cggccagctc ctggttgctg atgcgtccct gatgttgcaa
    37861 gagggtcaga atggcgcggt catgacgatc aagggtcatg gttgtcttct cgggttgatt
    37921 gttggaataa atatcatcaa acaagccaat ttgcgcaatt atattgccat atatcagctt
    37981 ttgattggtt gattagcaat cttctgccgc cgcagatgcc ttatgatgtc ccggtggaag
    38041 tgccgttcga cagcgcctct gttgtggtcg gcgcgctgcc gatgcccatc tttgtcagga
    38101 gagtgccatg agccagtttg accatcgcaa ataccgtccg attgctgcga tcgacctgcc
    38161 gcaccggcag tggcccagtc gcagcttcac caaggcgcca aagtgggcca gtgtcgacct
    38221 gcgtgatggc aaccaggcgc tgctggagcc gatgaatgtc atgcagaagc agcggatgtg
    38281 ggcgctgttg gtcaagctcg gcttcaagga gatcgaggtc ggattcccct cggccagcca
    38341 gcttgactat gacttcgtgc gctggctgat cgaggctgag cagattcctg acgatgtcac
    38401 cgttcaggtg ctggtgcagg cgcgcgaaga tctgatcgtg cgcaccttcg aagcgctggt
    38461 cggtgtgcga cgagcgattg tccatgtcta caactccacc tcgccagcgc agcgtgaacg
    38521 ggtgttcggg ctggatcgcg ccggagttac tgagatcgct cggcagggtg cgctctgggt
    38581 acagcgtgag gccgccaacc acccgcagac cgactggtgc tttcaataca gcccggagag
    38641 cttttccaat acagaagttg attacgccgt ggagatttgc gaggcggtgc tcgatgtctg
    38701 gcagcccacg cccgagcggc cctgcatcat caatctgccg agtaccgtgg aggtctcatc
    38761 gcccaacctt tttgcggacc aggtcgaata tttcgccacc cacatcagcc ggcgtgacag
    38821 catcatcctg tcggtccata cgcacaacga ccgtggctgc gcggtcgcag cggccgagct
    38881 ggcggtgttg gccggcgcgg atcgggtcga gggaacactg cttggcaatg gtgagcgcac
    38941 tggcaacatg gacatcgtga ccatggcgat gaacctctac agtcagggca tcgatccgga
    39001 gcttgatctc tccaaccctg acgaaatcat cgaggtttat atcgagtgca acaacctgcc
    39061 gatccatccc cggcatccct ggattggcga gttggtctac accgccttct ctggcagcca
    39121 tcaggatgcc atccgcaaat gtcttcacct gcaaaagccg gatgagcctt ggcaggtcgc
    39181 ctacctgccg atcgatccgc gtgacatcgg tcgcgactat caggccgtgg tacgggtcaa
    39241 cagtcagtca ggaaagggcg gggtcgcctt tgtgctcgag cgcgactacg gcctcattct
    39301 gccgcgctgg atgcaggttg aactggcgca gcaggtgcag gcgcagagcg agcgccaagg
    39361 gggtatcgtc gaaagcgcag ccatctatca actgctgctt gatcatttcg tcaatgatca
    39421 aagcccggtg cggctgctgg gttatcgtct ggatcgcaat ggccacgaca ccatcgaggc
    39481 acgattgaac gaggcagggc agaacaggag tctgctgggt agcggagatg gggcaatttc
    39541 agccttcatt gatgcatgga gtcgtcacag tcagcagcag gtcagcgtgg tcgactacca
    39601 cgagcacgcc gtcggcaccg gcaccgacgc tcaggcgatc gcctatgtcc agttgagcat
    39661 cgagggacgg cgtgtcagtg gggttgcgct cgaccaggat accgtcagcg cctcgctgaa
    39721 ggcggtgttg tcagcgctga accgctattg gggcctgagt cggcgggtgg cctgattgcg
    39781 cgtcatcggg gcagctgcag gtctgtaatg cgctaggttt cgtgatgtga ttgcatggcc
    39841 tggcgaagtg ctctaaccgt tcgatgcttt gaaaatcaat tggatctgtg catattttga
    39901 tctgatggcg cggttcctca tgtttgtcgg aggatcggcc gcgatctgtc cgccacggtc
    39961 gcggccgagt ggtgttgcgc ggggtgttgg tgagttaaaa tttccataag gcagggtgct
    40021 gcgtgcttga cagcagggtg ttgcgacggc atattccacc ttaaatttgt gggttatttg
    40081 ctaggctgga cgcctctcat gagcgcggtg ctcttgtagg tgggccagtg attgtctggt
    40141 agaattttac gataattaca aaaatctcaa cttcatcttc gcaagagtca acatggccgg
    40201 aatcataatc gtaggggcag ggcaggctgc cgtgcaggcg gctgccagtc tccgtcaaga
    40261 gggctgcaag gacgaaatca ccatttttgg cgatgagcca tggctgccct atcagcgtcc
    40321 tttgctttcc aagcagtatc tgctcggcac tgaaactgaa gagaaaatct atcagagggc
    40381 tgagaaattc tatgaagatc aaagggttgc cctgaggctt ggtgagcgaa tcgaaagcat
    40441 tgatgtcgcg gcaaaaactg tcaccagtgc caagggtgaa gtgatcccgt acaaagagct
    40501 gctgctggcg actggcagtc ggcccagaat cctcaagatt caggggagtg agctttccgg
    40561 tattcactac ctcagaacct tgggcgatgt gaatgccatc aagaacgaga tggccccggg
    40621 caagaggttg gtcatcattg gtggcggtta cattggtctt gaggttgcgt cggtcgctgt
    40681 gaccttgggg cttgatgtga cagtgctgga gatggaggag cgcattctca agcgggtgac
    40741 aactgcggag atgagtgagt tctaccacca gctgcacgcc gggcgtggtg tgaagatcct
    40801 gctcagaacg gcggtcagcg gttttgaggg cgatggtcac atcacgcatg tcctgtgcgg
    40861 agatcagcgc attgccgctg acatggtgat tgtgggcatt ggtatcatcc cgaatgtcga
    40921 gctggcccag gcggcgggca tcgagtgcaa caacggcatc gtggtcgacg accgctgccg
    40981 tacatcggct gagcacgtct atgcgctggg tgattgcagc aaccatccca gtccgctggt
    41041 cggcaggcgc ctgcgtctgg agtcggttcc gaatgcgcag gatcaggcca aggtcgtggc
    41101 tgcgaacatc atgggtaaac ccaagatcta ttcggctgtc ccttggttct ggtctgatca
    41161 gtacgaactg aagctccaga tggtgggttt ttctgccgac ggcgataccc aggtgctgcg
    41221 cggagacaaa tcaaccaatc agttcactgt tttttatcta aaggagggca aactggtcgc
    41281 ggtggacgcg gtgaacaatc cccgtgactt catgattggt cggcagtttt atggtcgaaa
    41341 aatggacgtt tcgaggcttt ccgatctgtc cgtggatgtt aaagacctgg tgttgccggt
    41401 cagtgcgggc tgatgcccgg attcggctat catcaaagtg tccaagctga gctgttgttc
    41461 aggcctgtcc agtccctggc tgtaggccga ttttataaac caacataatg aggagatgag
    41521 atgtccaagg ttacttttaa tctgtttaac ggagagcggc agacttacga tgctgcggat
    41581 ggggtttcgg tgatgcaggt cggcaaggac cacaacgtac cagggattga tgccgactgt
    41641 ggcggcgagt gcaactgcgc gacctgtcac gtttatgtcg atgaaaaatg gatgccgaag
    41701 gttggcatgc cgcatgacgc cgaagaggcg atcatgagtc tgaaccctga cagaaaaccg
    41761 aactcgcgcc tttcctgcca gatcaagatg agtgcggatc tggatggtct ggtggtgaat
    41821 ctgccagagt tccgatactg agcttgaagg ggtgcgccac ttgacgcttt gttttttttg
    41881 tgcaaagtgt tgagatgccc ctgtcaccca tttggaggtt cttcccatga cgactgcagt
    41941 gcagaaaaaa tacgaccaca tgtacggcaa ggatccatac agcatcccac tcgaggatta
    42001 cgatgtctcc cagccggagc tgttcgaatc caacaccatg tggggattct tcgaccgctt
    42061 gcgcaaagaa gatccggttc actactgcaa ggactccgaa aatggtcctt actggtcggt
    42121 gaccaagttc aaggacatca tggaggtaga aggcaatcct gaggtttact cttcggaacc
    42181 gacgatcagt ctggtcgacc caagagagga cttcaaagtc tcgatgttca tcgccatgga
    42241 tgagcccagg catggggagg agcgccgaac agtacaaggg gttgtggcgc cagccaatct
    42301 ggccagaatg gagccgctga ttcgtgaacg ggtgtgcggt attctcgatt ctctgcccgt
    42361 caacgagact ttcaactggg tcgacaaggt ttcgatcgaa ctgaccaccc agatgctggc
    42421 caccctgttc gattttccgt tcgagcagcg ccgcaagctg acctactggt cagatgtcgc
    42481 caccacgatt ccggcgccag gtgcactggt cgagtcgatc gaggagcgtc aaggcattct
    42541 gatcaatgag tgtctgcccg ccttcaccaa gctgtggaac gagcgggtca atgcgccacc
    42601 tcagtttgat ctgatctcaa tgctggccca tggtgaaagc acgcgccata tgcagcccta
    42661 tgagtttctg ggcaacctga tgctgctgat catcggtggc aatgacacca cccgtaactc
    42721 tctttccggc ggtgtgctgg cgctgaatga aaatccggag cagtatcaga agctgcgtga
    42781 caatcctgga ttgatcccca agctggtgcc agagatcatc cgctggcaaa ccccgctgac
    42841 cttcatgcgc cgtaccgcga cccaggacac catcctgcgc ggcaagcgga tcaagaaggg
    42901 tgacaaggtt gtgatgtggt atgtatcggg taaccgtgat gaagaggtca ttgaccgtcc
    42961 ctacgaattc atcatcgacc gcgagaaccc gcgtcaccac ctgtcgttcg gctttggcat
    43021 ccaccgctgc atgggcaacc ggcttgccga gatgcaactg cgcgtcgtct gggaagaggt
    43081 gctcaagcgc ttccataagg ttgaggtggt gggtaaacca gtgcgcaact actcgccctt
    43141 catcaagggc ttttcagacc tgccagttca gcttcgtccg ctgtaatttg gcatgtgatc
    43201 gagagatcgg tgttcgtcag ttgctggccc tgtgccagta actgacggct ccccatcgtt
    43261 tagagtccat tgaaattttg gttgttaccc agacaggcaa tatttcaatg gattgattat
    43321 ttatgggtca gtcaaaaggc atgccgtctg gtagaccttg ctgaccaacc gaaagcttga
    43381 gacgtattaa aaaacagatt ccaacatcaa gacggggggg catcatgagc acaggcgtag
    43441 acaaggtggc tgaacgggtc gtggagcagg cggcgcagga tccccgcagc atgtatggca
    43501 aggatccct
//
LOCUS       DAORCQ010000004        43223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_426_length_43223_cov_6.112861, whole genome shotgun sequence.
ACCESSION   DAORCQ010000004 DAORCQ010000000
VERSION     DAORCQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 43223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43223
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_426_length_43223_cov_6.112861"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..679
                     /locus_tag="PKI23_00605"
     CDS             443..679
                     /locus_tag="PKI23_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35468.1"
                     /translation="MMITPIASGLGILQRAEQQLNRHANTLARLSAEGNTSEVNQMTD
                     ALTGLKLVERDARLGIKVIEAENQRVGSLLDILA"
     gene            complement(711..1124)
                     /locus_tag="PKI23_00610"
     CDS             complement(711..1124)
                     /locus_tag="PKI23_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNN35469.1"
                     /translation="MNFVLDSSVTLPWFFKNDLTGYADAVLEQLQSNQVLVPRLWYEE
                     VANILLSWERQRGQSSPEAERFMAWLTELPIKVADDNSPRTFNRIYQLAHKYRISAYD
                     GVYLELAIREGLPLATLDTKLKRAAESAKVGVFSV"
     gene            complement(1121..1360)
                     /locus_tag="PKI23_00615"
     CDS             complement(1121..1360)
                     /locus_tag="PKI23_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNN35470.1"
                     /translation="MKNIGAYEAKTHFSRLLQEVMAGDGYIITHRGQPVAQLLPIGRE
                     TGQRAVPQVIADIRALAPSGGETVDLKKLRLEGRR"
     gene            complement(1429..2991)
                     /gene="ahpF"
                     /locus_tag="PKI23_00620"
     CDS             complement(1429..2991)
                     /gene="ahpF"
                     /locus_tag="PKI23_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNN35471.1"
                     /translation="MLDNSLKAQLKGFLERLVAPIELVASLDQSAKSQELHQLLTEIS
                     ELSDQVRLREDGSDPRRPSFSVGRLDQPARIRFAGLPLGHEFTSLVLALLQASGYPPK
                     ISDEQAARIAALRGRHRFESYVSLSCHNCPDVVQALNVLAALNPDIEHVMIDGALFQD
                     EVDARQVLAVPTLYLDGEPFGSGRMTLDELIARLDEGSAVREAERLSGQPPYELLVVG
                     GGPAAAAAAIYAARKGVRTGVVAERFGGQLLDTLAIENLIAVAHTEGPTLARTLEAHL
                     RQHQVDIINLQRADALIPGAPGGYHSLKLYSGAELRAKSLIIATGARWRELNVPGERE
                     YRGKGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVSQVTLIEFDSTLRADA
                     VLQEKLRSLPNVSVITRAQSREISGEAGRVNGLLYTDRATGQSRQIALEGVFVQIGLL
                     PNTDWLKGVVELSRSGEIIVDARGQTNVAGVFGAGDCTTVPYKQIVIAMGEGAKASLT
                     AFDYLIRTSAPA"
     gene            complement(3105..3671)
                     /gene="ahpC"
                     /locus_tag="PKI23_00625"
     CDS             complement(3105..3671)
                     /gene="ahpC"
                     /locus_tag="PKI23_00625"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNN35472.1"
                     /translation="MQSLINSEIKPFKAMMFHDGKFSEVSDADLKGKWSVLFFYPADF
                     TFVCPTELGDLADNYAEFQKLNVEIYSVSTDTHFTHKAWHDTSETIGKIRYPMIGDPT
                     GTLTRNFGVMIEEAGLAERGTFVIDPQGRIQIIEINAGGIGRDAGELLRKVKAAQYVA
                     NHPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            complement(3874..4707)
                     /locus_tag="PKI23_00630"
     CDS             complement(3874..4707)
                     /locus_tag="PKI23_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNN35473.1"
                     /translation="MSEQQAATLIRVEQGDDFVALVELCNPPFNFFELGMVQALGDAF
                     DRLDADPDCRAIVLAAAGQAFCAGANFGDGRDTDNALGGVPQQGAVTAGAGFSFSSAR
                     LYEQAVRLFGNRKPIVAAIHGAAIGGGLGLALVADFRVTCPQARFSANFTQLGIHPGF
                     GLTHTLPALIGPQRAQWLFYTGGRIKGDEAVAIGLADRLVEQDQVRDTARAMAREIAA
                     AAPLAVVSLRQTLRMGLAERVRAATQREQQEQAWLIQTADAREGMSASFARRTPRFEA
                     R"
     gene            complement(4852..5529)
                     /locus_tag="PKI23_00635"
     CDS             complement(4852..5529)
                     /locus_tag="PKI23_00635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNN35474.1"
                     /translation="MHETIELTASDGHRFALYQVLPSGAARGSVLVIQEIFGVNAHIR
                     ELCHRYAAAGYRAAAPALYDRVQRGVELNYDAAGIEAGRAIVAQIPQQKTLDDLATAV
                     AALRSTGRVGVVGYCYGGAMAWLAACKIDGVAAASCYYGGLIHQYRQLQPHCPVQLHF
                     GELDAMIPLTKVDEIAAAHPELPVYRYPADHGFGCDHRASYHAPSAELAEQRTLQLFA
                     EALVQGT"
     gene            5694..6155
                     /locus_tag="PKI23_00640"
     CDS             5694..6155
                     /locus_tag="PKI23_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HNN35475.1"
                     /translation="MHKGKMTMIMALTTTLGLGGCASQQGQQQQAGTVIGGVLGGVLG
                     SQVGGGDGRTAAIIAGSLAGAMIGGAVGKSMDDTDRLRTTQALENSRTGSASSWRNPD
                     SGNSYRVTPTRTYESSGGPCRDYVMEAQINGRPEQVHGTACRDANGTWVNQ"
     gene            6305..6847
                     /gene="hslV"
                     /locus_tag="PKI23_00645"
     CDS             6305..6847
                     /gene="hslV"
                     /locus_tag="PKI23_00645"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNN35476.1"
                     /translation="MEQFHGTTIISVRRAGRVVIGGDGQVTLGNTVLKGNARKVRRLY
                     QDRVLAGFAGGTADAFTLFERFEAKLEKYQGHLQRAAVELAKDWRSDRALRRLEALLA
                     VADHTHSLIITGNGDVVEPENALIAIGSGGPYAQSAARALLEHSELSARDIAEKALHI
                     AADICIYTNHQLTIEELESR"
     gene            6858..8186
                     /gene="hslU"
                     /locus_tag="PKI23_00650"
     CDS             6858..8186
                     /gene="hslU"
                     /locus_tag="PKI23_00650"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNN35477.1"
                     /translation="MPTLTPLQIVQELDQHIIGQAEAKRAVAVALRNRWRRMQLDETL
                     RAEVTPKNILMIGPTGVGKTEIARRLARLANAPFIKIEATKFTEVGYVGRDVESIVRD
                     LVEISIKQLREERLQRVGEQAELAAEERILDALLPPPRHESFEPRETVESSTRQTFRK
                     KLRNGDFDEKEIEIELSNRAVGVEIMAPPGMEEMTSQLQGLFSNLASGQKRKRRIKVA
                     VARKQLREEEASRLIDEEEVKARAVEAVEQNGIVFIDELDKVCKRGETVGADVSREGV
                     QRDLLPLIEGCTVSTKYGMVRTDHILFIASGAFHLAKPSDLIPELQGRLPIRVELQAL
                     TTEDFERILTEPSASLIRQYSALLATEGVELSFTPEAITTLARTAWEVNERTENIGAR
                     RLHTVLERLLEEISFDAGTGEAKPIVVDAAYVERHLGELARNEDLSRYIL"
     gene            8298..10064
                     /locus_tag="PKI23_00655"
     CDS             8298..10064
                     /locus_tag="PKI23_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNN35478.1"
                     /translation="MQNPLSLHQETRFLLHELFEVGRLWPQMPATRDLDAELVEQILD
                     EGGRLCDQLLAPLDRRGDEQGCRWVDGAVLTPAGFAEAWRSCAEAGWMGLGGEPEHGG
                     QGLPKLVTVLFEERLFAANSSFALYAVLTSGAALTLASHGTAAQRERYLPALYAGRCS
                     GAMCLTEPQAGSDLGLIRSRAEPDDNGQYLITGSKIFITGGEHDLSDDILHLVLARLP
                     DAPAGSRGLSLFLVPKFLPAASGGTGRRNRIFCRGIEQKMGIRASATCTIDYEGAMGE
                     LVGAPHQGLAAMFTMMNYERLSIALQGLGLGEAAYQAALAHARERQQGRAATTSLQRN
                     ELPADPIVLHGDVRHSLLTQKALNEGGRAFACYVALQLDLARFHPDAQQREQAAALVA
                     LLTPVAKAFMTDQGFENCVRAQQLFGGLGYMTATGIEQRLRDARIAQIYEGTNGIQAM
                     DLLERKVLRDGGAMLRHFCGEIDHCLAAERDNPPLAEFTQPLAAALARLRETSDWLIG
                     AAPRDAELPGAVASDYLKLVGLTAYAFVWTLTARAALRQSTPFHQGKVATARFFFARL
                     LPQSLGLAASIRNGASGPLPLF"
     gene            10149..11042
                     /locus_tag="PKI23_00660"
     CDS             10149..11042
                     /locus_tag="PKI23_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN35479.1"
                     /translation="MRDCPQLAEGTPFTHLFEEETLTLRGNEWLQRPERPILHFLHGV
                     AFCGHLYWPYLRELLPDCQLCLHDLEGHGASDRGQHYAGWRRNVDRLAEVIAARGWRQ
                     PGTPLIGGGHSYGAGLTLILAAEQPELFDALILLDPMIMPQAQLTNVLPFERNPAVQK
                     TLRRRRQWQNAEQARDFLRDKPAFADWTQQALDSFIDHNLQPEPDGSLTLRCDPVTEA
                     QVLADPLVTIWEHLQALRVPTFLLHGNDPRSPIPQNCQKAASLNPVISAIEIEGGHNF
                     MQERPAEVAALTRQLLEQLGH"
     gene            complement(11101..12480)
                     /gene="chrA"
                     /locus_tag="PKI23_00665"
     CDS             complement(11101..12480)
                     /gene="chrA"
                     /locus_tag="PKI23_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNN35480.1"
                     /translation="MSGVSPQPAQERELATVAAAESSIPFGQAVRFWLKLGFISFGGP
                     AGQIAIMHQELVGQRRWISEQRFLHALNYCMLLPGPEAQQLATYIGWLMHRTLGGIVA
                     GTLFVLPSLLILIALSWLYVSFGELPWVAALFYGVKAAVTAIILQAAHRLGNRTLHSP
                     LLWAIAGSAFVAIAVFELPFPAIVAAAALIGHLADRVTPGQFAAGNLPSHSAGSSGAA
                     LIDDDTPPPPHARFRWQRFVRSMVIGLLLWGASIGLLTLRFGWQHTLTQMGWFFTKAA
                     LLTFGGAYAVLPYVHQGAVEQFGWLTQTQMMSGLALGETTPGPLIMVVAFVGFVGGHG
                     KALFGPDALFLAGAAAATLVTWFTFLPSFLFILAGGPLVESTRGKLGYTAPLTAITAA
                     VVGVILDLVLFFARATLWPHGFSGSFDWLSALITLGAAIALLHYRRTTIEVIAGCALA
                     GLLGQVLPV"
     gene            complement(12485..14293)
                     /gene="oadA"
                     /locus_tag="PKI23_00670"
     CDS             complement(12485..14293)
                     /gene="oadA"
                     /locus_tag="PKI23_00670"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HNN35481.1"
                     /translation="MSRVQITDVVLRDGHQSLIATRLRTEDMLPVCAALDRIGFWSLE
                     VWGGATFDSCLRFLKEDPWERLRKLRRALPNSRLQMLLRGQNLVGYRHYSDSVVRAFV
                     QKSADNGIDVFRIFDALNDLRNLRVAIEAVNAAGKHAQGAISYTVSPVHSIEQFVTMA
                     RELEAMGCHSIAIKDMAGIMTPQAATQLVGALVKAVGVPIQVHSHATAGLASMSQLKA
                     VEAGATLLDTCISAFAEGPSHPTTESIVAALQGTAFDTGLDLAKLEEVAEYFRLVRKK
                     YWQFEAGVSGVDPRVLLHQVPGGMISNLINQLKEQGALERLDEVLAEIPRVRADLGYP
                     PLVTPTSQIVGTQAVINVMTGKRYQSVTKEVKQYLLGHYGAAPGVVDDQVRRSAVGDE
                     VPISCRPADLLPDEFDRLRSESEAFAASEEDLLTYAMYPELAENFLRERAAGTLAPEP
                     LLPPGFAASGPARTAPTDFNVTVHGETYNVRVTGSGHPGSGLRPYYVYIDGMPEEVMV
                     ETLGETGLSTAATGTLRPRPAHAGHLTTPMPGVIVAVNCQPGDQVKAGDPLLVVEAMK
                     MENEIQASVTGTIIAVHVHKGDSVHPDESLLEIQPA"
     gene            complement(14307..15725)
                     /locus_tag="PKI23_00675"
     CDS             complement(14307..15725)
                     /locus_tag="PKI23_00675"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNN35482.1"
                     /translation="MIRKILIANRGEIAVRIARACAEMGIKSVAIYTDADRHALHVKK
                     ADEAYSVGADPMQGYLNAHTIVNLAVASGCDAIHPGYGFLSENPLLAETCQRRGIRFI
                     GPSAEVIRRMGDKVEARKAMIAAGLPVTPGSEGNLAGVEQARTIAAQIGYPVMLKATN
                     GGGGRGIRQCADDAELLRNYERVVSEASKAFGKPEVFVEKCIVAPRHIEVQVLADAHG
                     NVVHLFERDCSIQRRNQKLIEIAPSPQLSPRQRQRVCTLAVEAARAVGYQNAGTVEFL
                     LDAQGDFYFMEMNTRLQVEHTITETITGLDIVQEQIRIAAGEPLSMQQDGISFRGFAA
                     EFRINAEDPQNSFLPSFGRVSRYYAPGGPGVRTDAAIYTGYVIPPYYDSMCVKLTAWA
                     LDWEALIARSRRALNDLSLSGVKTTIPYYLQILQHPAFRAGRFDTTFVDSHPELLAYS
                     LRRPHENIAAAISAVIAAQAGL"
     gene            16003..16320
                     /locus_tag="PKI23_00680"
     CDS             16003..16320
                     /locus_tag="PKI23_00680"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNN35483.1"
                     /translation="MPSVERNWAEKRDFIRMRLNSEVTLEIDQPPRTLRGRCRDLSGA
                     GLSVELDEALPTGTHFTAAFASIDGSRLDETAIQTRAEVARVERTANGHFLHGCLMLE
                     ILA"
     gene            complement(16342..17049)
                     /locus_tag="PKI23_00685"
     CDS             complement(16342..17049)
                     /locus_tag="PKI23_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNN35484.1"
                     /translation="MSGRAVRRFMMMALLFVPPAWSGEQQHDNPDPWEGFNRKVFVFN
                     DTFDRYLLKPLARGYMVVMPDWADRGLYNFFGNLSEPRVIVNGLLQGKFKQAGGDGLR
                     FVLNSTVGLLGFIDVASRFDLRKHDEDFGQSLGVWGLSSGPYLVLPLLGPSTVRDGVG
                     LAPDFMLDPIARIDEDETRYAVIGFKAVIVRAHLLSSEKLISGDRYLFIRDVYLQNRE
                     FLIKGQTRDPFLDESDE"
     gene            17343..18524
                     /locus_tag="PKI23_00690"
     CDS             17343..18524
                     /locus_tag="PKI23_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HNN35485.1"
                     /translation="MSRPVHVIGVGMTQFTKPGASEDYSVMGAKAIADALKDAGIDYR
                     EVQQAFAGYVYGDSCCGHKVVYSAGLTGIPIFNFNSNCSTGSSALAVARQAIAYGAIE
                     CALVVGFEQMTAGALKSYFGDRSSPGERHGATMARLQGVDMNAPGAAQMFGGAGREHM
                     KKYGTKAETLAKISVKARKHAANNPLALFNTPLSLEEIMAAPMVFEPLTRYQCCPPTC
                     GAGAAVLCSPEFAARHGLGNLVTIKAQAVTTDTESSFGDSMIKMVGYDMARSAAQQVY
                     QASGVDPQDIDVVELHDCFTANELISYESLGLCPEGGAEKLIEDNDNTYGGRFVVNPS
                     GGLLSKGHPLGATGLAQCYELVQQLRGRADRRQVEGARLALQHNLGIGGTCVVTLYER
                     G"
     gene            complement(18623..19288)
                     /locus_tag="PKI23_00695"
     CDS             complement(18623..19288)
                     /locus_tag="PKI23_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35486.1"
                     /translation="MSSSERRWPLWLQPWVTVALLALPIANWIKCPLLLLFWWWSFGP
                     TRRSERWLFGLSCLLFTVMNLMALRQGIFAFTSPDLLGLPWYEFFMWGFYLLHARRLL
                     GPACSAPATAVCWLLMGLFALCFTALSDPTWLLLASASLLAVTRWLDADPLAWRHAGY
                     LLLLGALFEYVGVHSGQWHYPGAPWGGVPLWFATMWGGIGFFAQRVAWSPLLRLDARQ
                     SAG"
     gene            complement(19304..20422)
                     /gene="ald"
                     /locus_tag="PKI23_00700"
     CDS             complement(19304..20422)
                     /gene="ald"
                     /locus_tag="PKI23_00700"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNN35487.1"
                     /translation="MIIGVPREIKTEEYRVGLTPHAVHELALHGHRLLVERGAGLGSG
                     FGDDDYQRAGATLIDSAAEIWARAALIVKVKEPQPGEYGLLRAGQLLFTYLHLAPDPT
                     LTQALLDSGASCIAYETVTSAGGQLPLLTPMSEVAGRLAIQAGAHALEKSQGGRGLLL
                     AGVPGVKPAKVVILGGGVVGSSAALIALGMGADVTLLDRSIERLRQLEQQFGGRLDTC
                     YATTHNIEREAVTADLIIGAVLIPGARAPRLISRTLLGSLNPGAVLVDVSIDQGGCFE
                     SSRPTTHRAPTYVEQGVVHYCVANMPSAAARTASLALNNATLPYVLELADAGLSAALL
                     RSPGLRAGLNVHQGRLTCQAVAEAQGRDYCAADTLFAA"
     gene            complement(20495..20770)
                     /locus_tag="PKI23_00705"
     CDS             complement(20495..20770)
                     /locus_tag="PKI23_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35488.1"
                     /translation="MMQPRRTPVEQPIILLSVYYPHHHDVKVIEWLLDHFHPRNARII
                     AAGPILQDMTRYAVLRFVSELDVRRFKNWAREQKYRIVDRDVRGEPL"
     gene            complement(20742..21209)
                     /locus_tag="PKI23_00710"
     CDS             complement(20742..21209)
                     /locus_tag="PKI23_00710"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNN35489.1"
                     /translation="MTDNPETLLFAYGTLRRAALITALIGRLPAAARAWLPDHAVVRL
                     RGTPYPCLRPRRGAVTPGTLYRGLAPDEWQRLDRYEGARYRRRSLRVTPHSGGAAVAA
                     AVYLLAPACRGQSGPPWRFERPGRAVRPRSSAPFSARFRFHGIEHDATAPHAR"
     gene            complement(21215..21853)
                     /gene="msrA"
                     /locus_tag="PKI23_00715"
     CDS             complement(21215..21853)
                     /gene="msrA"
                     /locus_tag="PKI23_00715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNN35490.1"
                     /translation="MSPANRSKLEPISAEQALPGRSQAMPVPALHTVLKTPLQPPFPA
                     GLQQVLFGMGCFWGAERLFWQTPGVYGTAVGYAGGFTPNPTYEEVCSGRTGHSEVVRV
                     LFDPERVSYPQLLTLFWERHDPTQGMRQGNDLGTQYRSAIYCFSDAQLAATLASRDQF
                     QQALQGAGLGRITTEIALAGPFYYAEPYHQQYLDKNPGGYCGLKGTGVSCPV"
     gene            complement(21868..22173)
                     /locus_tag="PKI23_00720"
     CDS             complement(21868..22173)
                     /locus_tag="PKI23_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNN35491.1"
                     /translation="MKVMIDLCVVPIGVGVSVSDHVAACQQIIAAAGLSHQLHPYGTI
                     IEGDWDEVFAVVKQCHQRVHAMGAPRIHTTLRVGTRTDRDQSMQQKVESVERKLKNL"
     gene            complement(22258..22500)
                     /locus_tag="PKI23_00725"
     CDS             complement(22258..22500)
                     /locus_tag="PKI23_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNN35492.1"
                     /translation="MSGFVLYSTSGCHLCELALQSALPLLTLRGEMLTEVEIADDEAL
                     LQRYGTTIPVLRRLADGAELGWPFDGEQLQVWLDRV"
     gene            complement(22497..23360)
                     /locus_tag="PKI23_00730"
     CDS             complement(22497..23360)
                     /locus_tag="PKI23_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN35493.1"
                     /translation="MSLVCTEKSFTVFGRTIAARFWPNPQGRPLLALHGWLDNAATFD
                     LLAPLLEGYQTCAIDFAGHGRSDHLPPGVPYSWFLQMNDAFAVADQLGWAQFTLVGHS
                     MGAHLGLYMAAAFPERIDRLVLIDAIGHPGLKSERIVAETRQALERLHGLENRTPPLY
                     ENFEAMLLPRMKTVGNITEAAARTLLDRGVMPCNGGLTWSSDSRLRYPDPARLTEEEV
                     QAFIQAVEAPTLLILGEQGWPWSADFIARRTAVHRNLQKLILPGGHHLHLEDSAPEVA
                     QAILDFLGRAA"
     gene            23562..24653
                     /locus_tag="PKI23_00735"
     CDS             23562..24653
                     /locus_tag="PKI23_00735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNN35494.1"
                     /translation="MTSLNQLLDNLRTNETLARKIFEIESEVLAIYNSRNFFDRLLAL
                     IQEKFGVPEAWVALIDEPITAPLIHHLSHCDQLTRSLVPLSAAGLRQLSGHFPDAWLV
                     SHCAERHNWLIPPYLQGMVRSMAVTPLQLEGRLIGALVQGDRKPNRWDPSMDTFFLSQ
                     LTVKVSLYLANLASREQLEYLATRDALTGLYNRREFDQALEREISRAQRSGAPLCAIF
                     IDCNDFKQINDTHGHDAGDAVLRHLAQGLEKLIRRVDCAFRYAGDEFVLLLPGQRQEQ
                     AQWVAQRLDHYFRAHPCPFRGQSIPYSISCGVASTEQSGIDSGTALLKQADMQLYQAK
                     GRKHEAVDHRLSTRIHALWHRLQKVFDTV"
     gene            24689..26158
                     /locus_tag="PKI23_00740"
     CDS             24689..26158
                     /locus_tag="PKI23_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNN35495.1"
                     /translation="MRQRIGLWLGPLLLLAILLLPRPEGLSSEAQRVGAVVAMMAIWW
                     LLDAVPVAITALLPILLYPLLGVMKSSEVTPSYANHLIYLFLSGFLLALAIEKWGLHH
                     RIALRTLAVMGHSPERVLLGFMLVTALLSMWMSATITAMMMLTVALALLHDEFGSAPS
                     PYTERGRDQRAFATSLTLGIAYAASIGGAVTIVGSPPNAILAGILAQQHEMSLSLIGW
                     MLMTLPVALVMLFGVWFYLSRIAYPTRFNHLPGGTALFHKAHQDLGDFSRAERRLIAV
                     FALVVVGWIVRALNQSEFFDWVQDSTIALAGAVALFVIPSGRRPGEPLLDWKSAKQLP
                     WEIILLFGGGFAIAQGFQETGLTEWLAGQLLNLPDIAPFWILLITTLFALALTEVTSN
                     ISTAALILPLVGAIAVIFDLPTQLLMSSAAIACSYAFMLPTATPANAIAYGSGYFSIG
                     QMARTGLPINLLGALLISGYLYLFMDAFAPLLFSSASNG"
     gene            26168..28171
                     /gene="mnmC"
                     /locus_tag="PKI23_00745"
     CDS             26168..28171
                     /gene="mnmC"
                     /locus_tag="PKI23_00745"
                     /inference="COORDINATES: protein motif:HMM:NF002481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNN35496.1"
                     /translation="MRDADRPFALPHAEIDWSADDEPFSSRFGDHYASRAGALAESRL
                     VFLDANRLGERWSGLRSGERFTLGETGFGTGSNFLLAWELWQRLAPAAARLHYLAVEA
                     HPLHPDDLARALTRHPPLTALSEPLLAAYPAAIPGIHRLELHAGTSPVTLDLLFGDAE
                     AMLAQLCTRQGFAIDGWFLDGFAPQRNPGMWHDGLYRQLARLSRPGTTFGTYTAAGAV
                     RRGLSDAGFRVERASGFGHKRERLLGHFVAATAPVVEPGPPWSWLPPARHAATASAPV
                     AVIGAGIAGSSTAQALARRGIEVALFDSENGAGRAASGNPQAVLYSKLSAHATPQSRF
                     ALHSYLHALRFYQALEQQRSAEQATLLHGCGLLQLATQAATRQRLLDIAHRSGLPTTL
                     VRWVDAAEASQLAGVALAQGGLLFPTGGWLTPTALCQQLSSHPRIRCHFGTAVTQLRH
                     DAEHGSWQLLDGDGHCLLRTATVVISAGAASTRFEPLAALPIKPIGGQVSQLPASSAS
                     RSLQLVLCGEGYLAPADQAAHTFGASYRLHDDDGLPSIDDHRRNLAMLQQEFPTFHQQ
                     LADEQLDPARLAGRYGIRATTPDRLPLVGPVTAGAQPIRHLPGLYINAGHGSRGFVQA
                     PLCAELIAALVSGDSLPVESTVMTALNPARFAVRALKHRKQQS"
     gene            28293..29852
                     /locus_tag="PKI23_00750"
     CDS             28293..29852
                     /locus_tag="PKI23_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN35497.1"
                     /translation="MSEPAYLNDPMAAAGCGMIVAFHANKNPNRVAIMADQGPTRSYG
                     ELNARINQIAHLMRANGMKAGDAVSLICSNRAEFVEALMACFRTGMRVSPVNWHLTAP
                     EMAYIVNDSESKVLFGDARFADTMAKVLKESPDVKLALSIGGDIPGFQNLESAIKEQP
                     TSNIDAPVRGFQMLYTSGTTGYPKGVYREAASSPSAITQYANYQPATDVTLCTGPLYH
                     AAPLGIDLLAALGNGLGVYLMDKWDPERTLQLIEKHKITHSHMVPTMFHRMLALPEEV
                     RKKYDLSSLKFIIHGAAPCPVHVKQQMLDWVGDIIYEYYGATEGVGTTITPAEWRKKP
                     GAVGLAPQGLIIVSEETDQELPPGEIGIIWIPVPPEGGFKYFKAEEKTQKAYRNGTHY
                     SMHDMGWVDEEGWLYLSDRRADLILAGGVNIYPAEVDAALLKHPAVSDACTIGVPNPD
                     WGQEVMAVIELKEGVKPSEELKQDLISFCQQNLAKFKCPRKIEFMDALPRSDAGKIQR
                     RKVRDIYIPAA"
     gene            30038..30349
                     /locus_tag="PKI23_00755"
     CDS             30038..30349
                     /locus_tag="PKI23_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35498.1"
                     /translation="MSSSAITPSTLPAPRSATPQWPVRAAERTSFATREAVAAKDSVE
                     TLRRPVWRESSPRGGTNLDPWQRTLTAQARAALRAYGEWATPPVTETPEVTLVGVDLY
                     V"
     gene            30346..31158
                     /locus_tag="PKI23_00760"
     CDS             30346..31158
                     /locus_tag="PKI23_00760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNN35499.1"
                     /translation="MTLQRVGILHDSTSCFAEQAEALAMRHQLPLIAIEQADSAALLL
                     ILDQDGLALHSMDAAGEQPLRIDFGHGRLDYRRRHGGGTQEPLARAVGLRGGFRPQLL
                     DATGGFGTDAFVLASLGCQVTLCERSPVMAALLADALARAGQKAELAAIVTRITLRQC
                     DARDELASGRQHDVICLDPMFPPRRKSALPRRTMVLLQQLVGHDEDADALLPLALERA
                     RYRVVVKRPRQAPPLAGLTAHHSLEGSSCRFDVYTLRKLPSKQEAAEAASTS"
     gene            complement(31147..31860)
                     /gene="tsaB"
                     /locus_tag="PKI23_00765"
     CDS             complement(31147..31860)
                     /gene="tsaB"
                     /locus_tag="PKI23_00765"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNN35500.1"
                     /translation="MARLLALETSSERCSVALWWEGALSERQVDLPREHNRQLLGLIE
                     ALMAEHDARFGELDAIAFGAGPGSFTGLRIACGVAQGLALGAEIGVLPVSSLAALAHA
                     AALRHPQQRVLTAVDARMGEIYWCGYQLQAQRPQPLLEEWLGPAAQIPLPAGLDLADT
                     VGVGNGWSQPQPLVEALAGWPAHIDAGLLPGAADVVALALPALARGELLPPEAAQPSY
                     LREQISWRKRADRGLSSGG"
     gene            complement(31838..33865)
                     /locus_tag="PKI23_00770"
     CDS             complement(31838..33865)
                     /locus_tag="PKI23_00770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNN35501.1"
                     /translation="MSQPPAVDAATGPPAIHPVAALLDRAGPFAAQLPGFMPRQSQMA
                     MAALVADTLEARDIAVIEAGTGTGKTFAYLLPAVISGKRVIISTGTKNLQQQLFFRDL
                     PALLRVLGLDLSSALLKGRGNYLCPHRLGLTLESVTLFEHEDRQLLGELAQWHSGGGS
                     GDLGDFPALDEAHPLWPRITSSADNCLGQECPDFSRCPLMEARREALKAELVVINHHL
                     LFADMVLKEEGVGELLPGAHALIIDEAHLLPEIAGQFFGERLSSRQLIELARDSEGAA
                     LKEAADDLPLRQLAAGLEQAARRLRRTLGPSEQRAPWNRLLDRAEFSAQADQLHEMLE
                     QLRQRLELVQGRGRELALCCERAGRLSTILATLLSEQAQESVRWYETSEQGFAFNRTP
                     LSVAEPFRQALTGYRAAWIFTSATLAVGQKFDHFIAQLGLDRLPRPPRGCVLPSPFDY
                     SRQALLYLPAQMPEPNQPDFTHCWIERLLPLLRASRGGAFLLFTSHRALREAATLLRQ
                     QIDLPLLVQGELSRRAMLERFIERDDALLLGASSFWQGVDIPGQSLRLVAIDKLPFAS
                     PDDPVTQARVDAMRRQGGNPFNDYQLPQAVVSLKQGVGRLIRGVTDRGVLVIGDPRLR
                     SRSYGRIFRQSLPPMPVTDYEAQAVEFLEALCSPLPAADSEMPDHGQTACP"
     gene            complement(33862..34308)
                     /locus_tag="PKI23_00775"
     CDS             complement(33862..34308)
                     /locus_tag="PKI23_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35502.1"
                     /translation="MLYQVDRLIEETRRIAAEYRRATGQVLPVSGELARHDAARLLGL
                     TLLHNAEQGVDAIDAAQLKYQIKSRVIFNQERGGQRIGQISLEGGWDRLLLVTMDASY
                     QPTAIYQLDREAASAAVGDGSAGRQKRGALSVARIKALATRVWSPT"
     gene            34354..35013
                     /gene="mobA"
                     /locus_tag="PKI23_00780"
     CDS             34354..35013
                     /gene="mobA"
                     /locus_tag="PKI23_00780"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNN35503.1"
                     /translation="MRDANSPDPALPRAQITGVILAGGAGQRLGGCDKGLHPLAGRPL
                     IAHVRERLAPQVGTLLICCNRHHDRYAQFGDRLISDSQPGHAGPLHGIAAALAVCTTP
                     YLAIAPCDTPALPAHWVARLGRQLLEHNAEVAVVHDGERLQSLCALWRVELAGAVADH
                     LAAGGSAPREFYRRCRSRWVDFSAEHAAFVNLNRAQEIAAYGNLIAQGHGITACRAGD
                     G"
     gene            complement(34985..35218)
                     /locus_tag="PKI23_00785"
     CDS             complement(34985..35218)
                     /locus_tag="PKI23_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNN35504.1"
                     /translation="MTQRIDQRLDVTGLNCPLPLLKAKQALNRLPADSYLEVVATDPG
                     SERDFQIFAKQSSYQLLRQWHEAEFIHHLLYKP"
     gene            complement(35268..35597)
                     /locus_tag="PKI23_00790"
     CDS             complement(35268..35597)
                     /locus_tag="PKI23_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35505.1"
                     /translation="MVFFSPKKSWAREELLHLLSLSGTLAGLCITGVTLLHGLAGSSR
                     MESLADEMLAFSALLFLLSCYLIFFALRHRESRHAQRLEMVADGLFFVALTGMVAAGF
                     VMVCLIR"
     gene            complement(35600..36277)
                     /locus_tag="PKI23_00795"
     CDS             complement(35600..36277)
                     /locus_tag="PKI23_00795"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNN35506.1"
                     /translation="MSSPSMLVGAPVVVALTGASGVQYGLRLIELLVAADRPIQLLLS
                     KAAHRVIEIETDWSLPANPAALQAALTERFRAAPGQIAAFGREDWLAPPASGSAPSAG
                     MVICPCSMGTLAAIAQGLSDNLIERAADVALKERRRLIVVPREMPYSEIHLEQMLRLT
                     RMGALLLPASPGFYHRPQRIEDLIDFVVARILTHLGIEQRLLPAWGYASAVQPVENEK
                     VSSHSGS"
     gene            complement(36274..37653)
                     /gene="mpl"
                     /locus_tag="PKI23_00800"
     CDS             complement(36274..37653)
                     /gene="mpl"
                     /locus_tag="PKI23_00800"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNN35507.1"
                     /translation="MHLHILGICGTFMGSLAILASELGHRVTGSDANVYPPMSDQLAA
                     RGIALHQGYDPAPLDARPDLVVIGNAMSRGNPAVEAVLNRGLDYCSGPEWLARHLLNR
                     RHVIAVAGTHGKTSTTSMVAWILECAGHDPGFLIGGVARDFGCSARIGSGSHFVVEAD
                     EYDTAFFDKRSKFIHYRPRTLLLNNLEYDHADIFPDLAAIQRQFHHLVRTVPGEGVII
                     APTADSALQEVLSEGCWSRRQAWLGCLERGETIPQSGWWARLVKEDGSAFVVGRDGVQ
                     RAEVQWSLLGRHNVANAVMAMAAAADVGVDPVVAAAALGRFQGVKRRLEVIGSGDGIT
                     VYDDFAHHPTAIATTLRGLRRRIGNQSLLLLIEPRSNTMRSGVHRRQLPAAWSSADAV
                     FWYQPEGMDWVPAADPTVPVHRFTSVTGMIERLVAERGRWQHLVIMSNGGFDGLPRRL
                     VEALGLAAP"
     gene            37823..39088
                     /locus_tag="PKI23_00805"
     CDS             37823..39088
                     /locus_tag="PKI23_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNN35508.1"
                     /translation="MTQMNAFYAQSGGVTAVINASACGVIECASRHSDRIGKVYAGRN
                     GILGALHEELIDTSQESASAIAALRHTPAGAFGSCRYKLKGLQENRAEYARLIEVFQA
                     HRIGYFFYNGGGDSQDTAHKVSQISVEMGHPIVCVGIPKTVDNDLPITDNSPGFGSVA
                     KYVAVSTREAGLDVASMSESSTKVFILEVMGRHAGWIAAAAGLAADKTGDPPHLIIFP
                     EIAFDEARFLAKVDASVKQHGYCVIVVSEGAHHADGRFLSDAGSRDAFGHLQLGGVAP
                     TLAALVKRELNYKYHWAVADYLQRAARHIASKTDVDQAYAVGEAAVEFALAGRNAVMP
                     TIVRQPGTVYRWSIGAAPLAEVANVERKMPRDYISADGFGITDACRDYLLPLIQGEDY
                     PPYHNGLPQYANLKKVLVPKKLPPFNPGK"
     gene            complement(39125..40048)
                     /locus_tag="PKI23_00810"
     CDS             complement(39125..40048)
                     /locus_tag="PKI23_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNN35509.1"
                     /translation="MSIAFITHPDCLLHDSGSHHPECPQRLSAIQDHLIETQLWDLLK
                     HYDAPLAEDQAIEAAHGRAHLQRIREAVPQSGLFYLDEDTAMGPDSLRAALRAAGAAV
                     RAVDLVLAGEVQRAFCSVRPPGHHAEHDRAMGFCLFNNIAIGTLHALHAHGLERVAVV
                     DFDVHHGNGTENIFQHDERVLFLSSFQYPFYPFTDINFPAENIIHAPIAAGAGGAEFR
                     EALRRRWLPYIDHFKPQLIMVSAGFDGHREDVMAHLRLDESDYQWITQELVTLAERHA
                     AGRIVSVLEGGYHLPALARSVAAHLRALGGF"
     gene            40151..42871
                     /locus_tag="PKI23_00815"
     CDS             40151..42871
                     /locus_tag="PKI23_00815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNN35510.1"
                     /translation="MEHRHYLASLFEPRSVAVIGATERPDAVGHFVMRNLLGHSYQGK
                     VFAINPNRDSIHGLLAHPDIESLPSTPEVAIIATPAHTVAELLRACGRKGVKFAVVYS
                     GGFAEIGAAGLDAQRELDAALRDSGVRILGPNGLGTVRPALGYDCSIGHGQVARGNLA
                     LVSQSGTLCNAIIDWAKVNRIGFSNIITLGSSLDVDVGEVLDYLINDSQTAGILIYLE
                     GVSHARRFISALRAASRVKPVIAIKVGRHQSGSSAAFSHTGALVGHDEVFDAILRRTG
                     VTRVQNIPELFAAARTLTSRYRECGERVAIVTNGGGPGALAADRAADLQLTLPALNHR
                     TLERLDQFLPRDWSRANPVDINGDATPERFRDAVAACLEDDEIDGVLVILTPQGMTAP
                     TQVAQALVELADRHAKPILCCWMGGETVREGRLRLMEARIPVFQYPETLVGGYHCLLT
                     HRKNQQLLLQMPAPTPDRAPPDLERARALIAEVRADGRSALNQQEAMALLAAFHIPVP
                     QAGIVQRVDAAIELAEKIGYPVAMKIHSPDIDHKSEVGGIALNLRTAAELVASYWQMM
                     RDIPKRLPDARLDGVLITAMATRPRARELLVGVTTDPIFGPVISLGTGGIWVEAYDHH
                     AVALPPLNEELALDLIRNVRFSHLLGAHRRFPAISQSALATVLIRVSELVEEFPEIAE
                     LDINPLLVDEQGTIAVDARVYLREPAIQCDPYDHLAICPYPKHLIRTWQLRDGTQVTV
                     RPIRPKDAEMEQNFVRNLSDRSRYLRFWSGLSELNRRMLAEFTKIDYGTRMSLIVTLE
                     QDGQEIEIAVAQFSTTATGQSCEFAIVVADGWQGQGLGGKLMAYLIEIARCRGYRSIE
                     GEVLSENRDMLNLMRALGFKVEIDPEDLSVRRVVKNLLEPDPPRRGPASA"
     gene            complement(42884..42959)
                     /locus_tag="PKI23_00820"
     tRNA            complement(42884..42959)
                     /locus_tag="PKI23_00820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42924..42926),aa:Thr,seq:cgt)
BASE COUNT         7814 a        14809 c        13649 g         6951 t
ORIGIN      
        1 agcccggcca gtcggggtgg gcattgcggg cgatgccggc gtaggtctgc ccgccacggt
       61 cgccctcgat gttggtgagc ttgaatccgc cttcattgcg gatcatcttg tcaaaggcgg
      121 ggtcgaaggt ggccatgtcg caactccttg gctgtttcaa tccgtccccg gcttttcggc
      181 cgggccaaac tggcagtgct ctgtttacct catctcgttc atgctgaaaa gccccctgcc
      241 ccgtggagca gcccgcacgc gcgcctgtgc agacttgaat ccggtgagca ggcactgcct
      301 gctgtcgcga agggactgaa gcgtgccacc ctggcgacca gacccgataa tgccaacccg
      361 ttctcatcct cgaccatttc tgctcgcggc agccgcattg gcgttctatc ttgccgaaca
      421 tgttcaatag cggagcgaag cgatgatgat cacgccaatc gccagcggcc ttggcatcct
      481 gcaacgggcc gagcagcagt tgaatcgcca tgccaacacc ctggcccggc tcagtgccga
      541 aggcaacaca tcggaagtca atcagatgac cgacgcactg acaggactga agctggtcga
      601 acgtgatgcc agactcggca tcaaggtgat cgaggccgaa aaccaacggg tcggcagtct
      661 gctcgatatt ctggcctgaa cggtcaggct ggctgaggaa tcctgtgacg tcagaccgaa
      721 aagacgccaa ccttggcgct ctcggccgcc cgtttcagtt tggtgtcgag tgtcgccagc
      781 ggcaacccct cacggatcgc cagttcgaga tagacgccgt cataagctga aatgcgatat
      841 ttgtgtgcca gttgatagat gcggttgaag gtgcgcggcg agttgtcatc cgccaccttg
      901 atcggcagtt cggtcaacca ggccatgaag cgctcggcct ccggcgagct ctgcccccgc
      961 tgtcgttccc atgacagcag gatgttggcc acctcttcat accacaggcg tggcaccagc
     1021 acctggttgc tctgcaactg ttcgagcact gcatcggcat agccggtgag gtcattcttg
     1081 aagaaccacg gcagcgtcac cgacgaatcg agcacgaaat tcatcgccgt ccctccagac
     1141 gcagtttttt cagatcgacc gtctcgccac cgctcggcgc cagcgcccga atgtcggcga
     1201 tcacctgcgg cacggcccgc tgaccggtct cgcgaccgat cggcagcaac tgcgccaccg
     1261 gctgtccgcg atgggtgatg atgtagccat caccggccat gacctcctgc agcagcctgg
     1321 aaaaatgggt tttcgcctcg taggcaccga tgtttttcat gtcgctccct ccatgtgtgc
     1381 gaaccagtct atggaactgg tcggctcgcc gcaaccgcgg ccacggaatc aggcaggcgc
     1441 agaagtgcgg atcaggtaat cgaaggcggt cagactggcc ttggctcctt cgcccatcgc
     1501 gatgacgatc tgtttgtaag gcacagtggt gcagtcgccg gcaccgaaca cacccgcgac
     1561 gttggtctgt ccgcgggcat cgacgatgat ctcgccactg cgcgacagct cgacgacccc
     1621 cttcagccag tcggtgttgg gcagcaggcc gatctgcaca aacactcctt ccagcgcgat
     1681 ctgccggctc tgaccggtgg cgcgatcggt gtagagcagt ccgttgaccc ggccggcctc
     1741 accgctgatc tccctgctct gcgcacgggt gatcacgctg acattgggca ggctgcgaag
     1801 cttttcctgc aacaccgcat cggcccgcag cgtactgtcg aactcgatca gcgtgacctg
     1861 gctgacgatg ccggccagat cgatcgccgc ctcgacgccc gagttgccgc cgcccaccac
     1921 ggcaacacgc ttgcccttga acagcggtcc atcgcagtgc gggcagtagg ccaccccctt
     1981 gccgcggtat tcgcgctcgc ctggcacatt cagctcgcgc cagcgcgctc ctgtggcgat
     2041 gatcaggctt ttcgccctca gttcggcccc actgtacagt ttcaggctgt ggtagccgcc
     2101 gggcgcgccc ggaatcagcg catcggcacg ctggaggttg atgatgtcga cctggtgctg
     2161 ccgaagatgc gcctccagcg tgcgcgccag cgtcggcccc tcggtatgcg ccactgcaat
     2221 cagattctcg atcgccaggg tatcgagcag ttgcccaccg aaccgctcgg cgaccacgcc
     2281 agtgcgaacg cccttgcgcg ccgcatagat ggcggccgcc gcggcagccg gcccaccacc
     2341 caccaccaga agttcatagg gcggctgccc ggagagtcgc tcggcctcgc gcacggcgct
     2401 cccttcatcc agccgggcaa tcagctcatc gagggtcatg cgtccgctgc cgaagggctc
     2461 gccatcgagg tagagcgtcg gcaccgccag cacctggcgc gcatcgacct catcctgaaa
     2521 cagcgcgcca tcgatcatca catgctcgat gtccggattc agcgccgcca gcacattgag
     2581 cgcctgcacc acatcaggac agttgtggca cgacagcgac acatagctct cgaagcggtg
     2641 tcgacctctg agggccgcga tccttgccgc ctgctcgtcg gaaatctttg gcggatagcc
     2701 gctggcctgc aacagcgcca gcaccagcga tgtgaactcg tggcccagcg gcagcccggc
     2761 aaaacgaatg cgcgccggct gatcgagtcg cccaacactg aacgagggtc gacggggatc
     2821 actgccatcc tcgcgcagcc gaacctggtc ggaaagctcg ctgatctcgg tgaggagctg
     2881 gtgcagctcc tgtgatttgg cgctctggtc gagcgaggcc accagttcga tcggagcgac
     2941 cagtcgctcc agaaagcctt tcaattgtgc cttgaggctg ttgtcgagca tcagaatctc
     3001 ccgccatgaa tttgcatggc aacaaaaagg cgccggcagc cgcacggcgg ccgacgcctc
     3061 ggttcagatt cggctgcccc gcccgctggg aggggcaggc atcatcagat cttgcccacc
     3121 agatcgagcg agggcgccag tgtggcttca ccctccttcc acttcgccgg gcagacctcg
     3181 ccggggtggt tcgccacata ctgcgccgcc ttcaccttgc gcagcagctc cccggcatcc
     3241 cgaccgatgc caccggcatt gatttcgatg atctggatgc ggccctgcgg atcgatcacg
     3301 aaggtacccc gctcggccag accggcctcc tcgatcatca ccccgaaatt gcgggtcagg
     3361 gtgccagtgg gatcgccgat catcgggtac ctgatcttgc cgatggtctc ggaggtgtca
     3421 tgccacgcct tgtgggtaaa gtgggtgtcg gtcgacaccg aatagatctc cacattcagc
     3481 ttctggaact cggcgtaatt gtcggccagg tcgcccagct cggtcgggca gacgaaggtg
     3541 aaatcggccg gatagaaaaa cagcaccgac cacttgccct tcagatcggc atcgctgacc
     3601 tcggaaaatt tgccgtcatg gaacatcatt gccttgaacg gcttgatttc gctgttgatg
     3661 agactctgca tacttcagta ctccgatgag aaagcgggac aaaacgtcac gaaagatgac
     3721 ggtggccact ctatcgacgc cggtcggcgc cggccaattc aatttttaaa tgggtgcgat
     3781 agccaatttc aatcggacag ccgcacggcc ggccgcgcgg cccgctggca gtgatggggt
     3841 gatgacagga agaggatgga tgggcgcgcc ggttcagcgc gcctcgaacc ggggcgtgcg
     3901 ccgggcaaag ctggcgctca tgccctcacg ggcgtcggcg gtctggatca gccaggcctg
     3961 ctcctgctgc tcgcgctggg tggccgcccg cacccgctcg gccagtccca tccgcagcgt
     4021 ctgtcgcagc gacaccaccg ccagcggcgc ggcggcggca atctcgcggg ccatcgcccg
     4081 ggccgtgtcg cgcacctgat cctgctccac cagccggtcg gccaggccga tggcaacggc
     4141 ctcgtcgccc ttgatgcgac cgccggtgta gaacagccac tgcgcccgct gcggaccgat
     4201 cagcgccggc agcgtgtggg tcagaccaaa accggggtgg atgcccaact gggtgaagtt
     4261 ggcgctgaag cgcgcctgcg ggcaggtgac gcgaaaatcg gccaccagtg ccagccccag
     4321 accaccgccg atcgcggcgc catggatggc cgcgacgatc ggcttgcggt tgccgaacag
     4381 ccgcaccgcc tgctcataga gtcgcgccga gctgaagctg aagccagcac cggctgtcac
     4441 cgcgccctgc tgcggcacgc cacccagcgc gttgtcggtg tcgcggccat caccgaagtt
     4501 ggcgccggcg cagaaggcct gaccggccgc cgccagcacg atggcgcggc agtcggggtc
     4561 ggcatcgagc cggtcgaagg catcgcccag cgcctgcacc atgcccagtt cgaagaagtt
     4621 gaacggggga ttgcacagct cgaccagggc aacgaagtcg tcaccctgct cgacgcggat
     4681 gagcgtggcg gcctgctgct cggacatggt cgttctccga ttgcgaactc gacggccatt
     4741 gcagtgtggc atgaggctgt gcgccacgac aaacatggca tcactctgtc gatgcagctc
     4801 cgcatcgcca tcttgagttt gtgggcaatg ggttctccaa ggactgcgtg atcatgttcc
     4861 ctgtaccagc gcctcggcaa agagttgcag cgtgcgctgt tcagccagtt ctgccgaagg
     4921 ggcgtggtag ctggcgcggt ggtcgcagcc gaatccatgg tcggccggat agcggtacac
     4981 cggcagttcg gggtgggcgg cggcgatctc gtcgaccttg gtcagcggaa tcatcgcgtc
     5041 cagttcgcca aagtggagct gcaccggaca gtgtggctgc aactggcgat attggtgaat
     5101 caggccgccg tagtagcagg aggccgccgc cacgccgtcg atcttgcagg cggccagcca
     5161 ggccatggcg ccgccatagc agtagccgac cacgccgacc cggccggtcg aacgcagtgc
     5221 cgccaccgca gtggccagat cgtccagcgt cttctgttgc ggaatctgcg ccacgatggc
     5281 gcggccggct tcgatgccgg cggcgtcgta gttcagctcg acgccccgct gcacccggtc
     5341 atagagcgcc ggcgccgcgg cccggtatcc cgccgccgca tagcggtggc acagctcacg
     5401 gatgtgggca ttgacgccga atatctcctg gatcaccagc acgctgcctc tggccgcgcc
     5461 gctcggcagc acctgataaa gggcaaagcg gtggccatca ctggcggtca gctcgatggt
     5521 ctcatgcatg ggcggggctc ctgtgtggtt gccggaaggt gcgggagcag aaaatccacg
     5581 cgctgtcccg cagcgctttt gacccggaat caaaacaaat cggccaccca cgggtcaaac
     5641 cgggcgggat cgacgatccc gacgatcaat caacccggaa caagagagca gcaatgcaca
     5701 aaggcaagat gacgatgatc atggccctga ccaccaccct tggcctcggc ggttgcgcca
     5761 gccagcaggg acagcagcaa caggcaggca cggtgatcgg tggcgtgctg ggaggggtgc
     5821 tgggttcgca ggtgggcggc ggcgatggac gtaccgcggc catcattgcc ggatcgctgg
     5881 ccggtgccat gatcggcggt gcggtcggca agtcgatgga tgacaccgac cggctgcgca
     5941 ccacccaggc gctggagaac agccgcaccg gcagcgcatc gagctggcgc aaccccgaca
     6001 gcggcaacag ctaccgcgtc accccgaccc gcacctacga gtcgagcggc ggaccctgcc
     6061 gcgactacgt gatggaggcc cagatcaatg ggcggccgga acaggtgcat ggcaccgcct
     6121 gtcgcgatgc caatggcacc tgggtcaacc agtaggccgc gcacgaccgg atcggtcggc
     6181 atctcgcgcc aatcgatccg gcctctcccc gccccggcac tgaattgccg gagcccgact
     6241 tgaaaagccg gcggatgccc tcatcacgcc acttctgact tttggcacgc atgaggagcc
     6301 acgcttggaa cagtttcatg gcacgacgat catctcggtc cggcgcgccg gccgggtggt
     6361 gatcggcggt gacgggcagg tcaccctggg caacacggtg ctcaagggca acgcgcgcaa
     6421 ggtgcgccgg ctctatcagg atcgggtgct ggccggtttt gccggcggaa ccgccgacgc
     6481 cttcacgctg ttcgagcggt tcgaggccaa gctggagaag taccagggcc acctgcagcg
     6541 cgccgccgtc gagctggcca aggattggcg cagcgaccgc gccctgcgcc ggctcgaggc
     6601 gctgctggcg gtggccgacc acacccactc gctgatcatc accggcaacg gcgatgtggt
     6661 ggagcccgag aacgcgctga tcgccatcgg ctcgggcggc ccctacgcgc agtcggcggc
     6721 gcgggcgttg ctcgaacaca gcgagctgtc ggcgcgcgac atcgccgaga aggcgctgca
     6781 cattgccgcc gacatctgca tctacaccaa ccatcagctc acgatcgaag agctggagag
     6841 ccgctgagcc agaccccatg cccactctga ccccgttaca gatcgtccag gaactcgacc
     6901 agcacatcat cggccaggcc gaggccaagc gtgccgtggc ggtggcgctg cgcaaccgct
     6961 ggcgacgcat gcagctcgat gagacgctgc gcgccgaagt gaccccgaag aacattctga
     7021 tgatcggccc caccggggtc ggcaagaccg agatcgctcg ccggctggcc cggctggcca
     7081 acgcgccctt catcaagatc gaggcgacca agttcaccga agtgggttat gtcggccgcg
     7141 atgtcgagtc gatcgtgcgc gacctggtgg agatctccat caagcaactg cgcgaggagc
     7201 ggctgcagcg ggtcggcgag caggccgaac tggccgccga ggaacggatt ctggacgctc
     7261 tgctgccgcc accacgccac gagagtttcg agccgcgcga gacggtggag tcctcgaccc
     7321 ggcagacctt ccgcaagaag ctgcgcaatg gcgatttcga cgaaaaggag atcgagatcg
     7381 agctgtcgaa ccgcgccgtc ggcgtcgaga tcatggcgcc gccgggcatg gaggagatga
     7441 ccagccagtt gcaggggctc ttctccaacc tcgccagcgg ccagaagcgc aagcggcgca
     7501 tcaaggtggc ggtggcccgc aagcagttgc gcgaagagga ggcgagccgg ctgatcgacg
     7561 aggaggaggt gaaggcgcgg gcagtcgagg cggtcgagca gaatggcatc gtcttcatcg
     7621 atgaactcga caaggtctgc aagcgtggcg aaacggtggg ggccgatgtc tcccgcgagg
     7681 gggtgcagcg cgacctgctg ccgctgatcg agggctgcac cgtcagcacc aagtacggca
     7741 tggtgcgcac cgaccacatt ctgttcatcg cctccggcgc cttccatctg gccaaaccct
     7801 ccgacctgat tccggagctg caggggcgcc tgccgatccg cgtcgagctg caggcgctga
     7861 ccaccgagga tttcgagcgc atcctgaccg aaccgagcgc ctcgctgatc aggcagtaca
     7921 gcgcgctgct ggcgaccgaa ggggtcgaac tcagcttcac ccccgaggcg atcaccaccc
     7981 tcgcccgcac cgcctgggag gtgaacgagc gcaccgagaa catcggcgcc cgccggctgc
     8041 acacggtgct ggagcggctt ctggaagaga tctcgttcga tgccggcacc ggcgaggcca
     8101 agccgatcgt ggtggatgcc gcctatgtcg agcgccatct gggcgaactg gcgcgcaacg
     8161 aagatctgag ccgctacatc ctctgacgcc gccccggcag gccgaacgga accgatcggc
     8221 ctgccgcgct acagcttcga cgcactttgc ggcacactgg gctgcctcac cccagcctgc
     8281 caccgtcgag agtcgccatg cagaacccgc tgagcctgca ccaagagacc cgctttctgc
     8341 ttcacgagct gttcgaggtc ggccggctct ggccgcagat gccggccacc cgggacctcg
     8401 acgccgaact ggtggagcag attctcgacg aaggcggccg gctctgcgat cagctgctgg
     8461 cgccactcga ccgccggggc gatgaacagg gctgccgctg ggtcgatggc gcagtgctga
     8521 cgccggcggg ctttgccgag gcctggcgca gctgtgccga agccggctgg atgggattgg
     8581 gcggcgagcc ggagcatggc ggacagggcc tgcccaagct ggtgaccgtg ctgttcgaag
     8641 aacggctgtt cgccgccaac agcagctttg ccctctatgc ggtgctcacc agcggtgccg
     8701 ccctcaccct cgccagtcat ggcactgcgg cacagcgcga gcgttacctg ccggcgctct
     8761 atgcgggccg ttgcagtggc gcgatgtgcc tgaccgaacc gcaggccggc agcgacctgg
     8821 ggctgatccg cagccgggcc gagccggatg acaacggcca gtacctcatc actggcagca
     8881 agatcttcat caccggcggc gagcacgacc tcagcgacga catcctccat ctggtgctgg
     8941 cccgcctgcc cgatgcaccg gccggcagcc gcggtctgtc gctgtttctg gtgccgaagt
     9001 tcctgcctgc ggccagcggt ggtaccggcc ggcgcaaccg gatcttctgc cggggcatcg
     9061 agcagaagat gggcatccgc gcctcggcca cctgcaccat cgattacgaa ggagccatgg
     9121 gtgagctggt cggcgccccg catcaggggc tggcggcgat gttcacgatg atgaactacg
     9181 agcggctgtc gatcgcgctg caggggctcg gccttggcga ggcggcctat caggccgcgc
     9241 tggcccatgc gcgggagcgg cagcaggggc gtgccgccac cacctcgctg caacggaatg
     9301 aactgccggc cgatccgatc gtgctgcatg gtgacgtgcg ccactcgctg ctgacgcaaa
     9361 aggcgctgaa cgaagggggg cgcgccttcg cctgctatgt cgcgctgcaa ctcgacctgg
     9421 cccgctttca ccccgatgcg cagcagcgcg aacaggccgc tgcgctggtc gcactcctga
     9481 ctccggtggc caaggccttc atgaccgatc agggcttcga gaactgtgtg cgcgcccagc
     9541 agctgttcgg tggactgggc tacatgaccg ccaccggcat cgagcagcgg ctgcgcgatg
     9601 cacgcatcgc ccagatctac gagggcacca acggcatcca ggcgatggat ctgctggagc
     9661 gcaaggtgct gcgcgatggc ggcgcgatgc tgcggcactt ctgcggcgag atcgaccact
     9721 gcctggccgc tgaacgggac aacccgccac tggcggagtt cacccagccg ctggcggcgg
     9781 cgctggcgcg gctgcgcgag accagcgact ggctgatcgg ggcggcaccg cgcgatgccg
     9841 aactgccggg cgcggtggcc agcgactacc tgaagctggt cgggctgacc gcctatgcct
     9901 tcgtctggac gctgacggcc cgcgcggccc tgcgccagtc gacgccattc catcagggca
     9961 aggtcgccac cgcccgcttc ttcttcgccc gactgctgcc gcagagcctc gggctggccg
    10021 cttcgatccg aaacggcgcc tccggcccgc tgccgctgtt ctagacagtc gcatcgatcc
    10081 gttaccatct gcatcgcagc ggtacacggc cgccgtccat gagatgtcaa cccgatcagg
    10141 aaccgacgat gagagactgc ccgcaactgg ccgaaggcac gcccttcacc cacctttttg
    10201 aagaagagac cctgacactg cgcggcaacg agtggctgca acggccagaa cggccaatcc
    10261 tgcactttct gcatggcgtc gccttctgcg gccacctcta ctggccctat ctgcgtgaac
    10321 tgctgcccga ctgtcagctc tgcctgcacg atctggaagg ccatggcgcc agcgaccgtg
    10381 gccagcacta tgccggctgg cgccgcaatg tcgatcggct ggccgaggtg atcgccgcgc
    10441 gcggctggcg tcaaccgggc accccgctga ttggcggcgg ccacagctat ggcgccggtc
    10501 tgacgctgat tctggcggcc gagcagcccg agctgttcga tgcgctgatt ctgctcgacc
    10561 cgatgatcat gccccaggca caactgacca acgtgctgcc gttcgagcgc aatccggcgg
    10621 tgcagaagac actgcgccgg cgccggcagt ggcagaacgc cgagcaggca cgggacttcc
    10681 tgcgcgacaa accggccttc gccgactgga cccaacaggc cctcgacagc ttcatcgacc
    10741 acaacctgca accggagccg gatggcagcc tgacactgcg ctgcgaccct gtcaccgaag
    10801 cccaggtgct ggccgacccg ctggtgacca tctgggagca cctgcaggcg ctgcgcgtgc
    10861 cgacctttct gctgcatggc aacgatccac gctcgccgat tccgcagaac tgccagaagg
    10921 ccgccagcct gaacccggtc atcagcgcga tcgagatcga gggcggccac aacttcatgc
    10981 aggagcgtcc cgccgaggtc gccgcgctga cccggcaact gctcgaacag ctcggtcact
    11041 gaaacgcagg catcgccacg ttattcactc gaagccttcg ccctgcagat catcggctgc
    11101 tcagaccggc agcacttggc cgagcagtcc agccagggca cagcccgcga tcacctcgat
    11161 cgtcgtgcgc cggtaatgca gcagcgcaat cgccgcacca agcgtgatca gtgccgacag
    11221 ccaatcgaag gaaccggaaa agccgtgcgg ccagagggtg gcgcgggcaa aaaagagcac
    11281 cagatcgagg atgacaccga ccacggccgc agtgatggcg gtcagtggcg cggtgtagcc
    11341 gagcttgcca cgggtagact ccaccagcgg gcccccggcc agaatgaaca ggaaagaggg
    11401 caaaaaggtg aaccaggtga ccagggttgc cgccgctgcg cccgccagaa acagcgcatc
    11461 cggtccgaac agcgccttgc cgtggccgcc gacgaaaccg acgaaggcca ccaccatgat
    11521 caggggtccc ggtgtcgtct cacccaaagc cagaccgctc atcatctgcg tctgggtcag
    11581 ccagccaaac tgctccaccg ccccctgatg gacatagggc agcaccgcat aggcaccgcc
    11641 gaaggtgagc agggccgcct tggtgaagaa ccagcccatc tgggtcaggg tgtgttgcca
    11701 gccgaaccgc agggtcagca ggccgatcga cgcaccccac agcagcagtc cgatcaccat
    11761 gctccgcaca aaccgctgcc agcgaaatcg tgcatgtggc ggcggcgggg tgtcatcgtc
    11821 gatcagcgcc gcgccggagc tgcctgcgga gtggctcggc agattgccgg cagcgaactg
    11881 gccaggcgtg acacgatccg ccagatggcc gatcagcgcc gctgcggcga cgatggccgg
    11941 aaaaggcagc tcgaaaacgg cgattgccac aaaggcgctc ccggcaatcg cccacagcaa
    12001 tggactgtgc agcgtgcggt tgccgaggcg gtgggccgcc tgcagtatga ttgcggtgac
    12061 cgcggccttg acaccataaa agagtgccgc cacccagggc agttcgccaa agctgacata
    12121 gagccatgac agcgcgatca gaatcagcag cgacggcaac acgaacaggg tgccggcgac
    12181 gatgccgccc agcgtgcggt gcatcagcca gccgatgtag gtggccagtt gctgtgcttc
    12241 ggggcccggc agcagcatgc agtagttgag cgcgtgcaga aaacgctgct cgctgatcca
    12301 gcgccgctgc ccgaccagct cctgatgcat gatcgcaatc tgtcctgccg ggccgccgaa
    12361 gctgatgaaa ccgagcttga gccagaagcg cacggcctgc ccgaacggaa tggagctctc
    12421 cgcagccgcg accgtggcca gttcacgctc ctgtgcgggc tgcgggctca cgccgctcat
    12481 cggatcaggc tggctgaatc tccagcagcg actcatcggg gtggacgctg tcgcccttgt
    12541 gcacatggac cgcgatgatg gtgccggtga ccgaggcctg gatctcgttc tccatcttca
    12601 tcgcctccac caccagcagc ggatcaccgg ccttgacctg atcgcccggc tggcagttga
    12661 ccgcgacgat cacccccggc atcggcgtgg tcaggtgacc ggcatgggcc ggacggggcc
    12721 gcagcgtgcc ggtcgctgcc gtcgacagcc cggtttcacc gagggtctcg accatcacct
    12781 cttccggcat gccgtcgatg tagacatagt agggacgcaa tccgctgccg ggatggccgc
    12841 tgccggtgac ccggacattg taggtctcgc catgcacggt cacattgaaa tcggtcggcg
    12901 cagtgcgcgc ggggcccgat gcggcaaagc ccggcggcag cagtggctcg ggggccagcg
    12961 tgccggcggc gcgctcgcgc aggaagttct cggccagctc cggatacatc gcgtaggtga
    13021 gcagatcctc ctcgctggcg gcgaaggctt cgctctcgct gcgcagccgg tcgaactcat
    13081 cgggcagcag gtcggccggt cggcaactga tcggcacctc gtcacccacc gcgcttcggc
    13141 gcacctggtc atcgaccacg cccggcgcag caccatagtg gccgagcaga tactgcttca
    13201 cctccttggt gaccgactgg taacgcttgc ccgtcatcac attgatcacc gcctgggtgc
    13261 cgacgatctg cgaggtgggg gtgaccagcg gcggatagcc aagatcagcg cgcacccgcg
    13321 gaatctccgc cagcacctcg tcgaggcgct ccaaggcgcc ctgctccttc aactgattga
    13381 tcaggttgga gatcatgccg ccgggcacct gatgcagcag cacgcgtgga tcgacaccac
    13441 tgacgccggc ctcgaactgc cagtacttct tgcgcaccag gcggaagtat tcggccacct
    13501 cctccagctt tgccagatcg agcccggtgt cgaacgcagt gccctgcagt gcggcaacga
    13561 tgctctcggt ggtggggtgg ctcggcccct cggcgaaggc ggagatgcag gtgtcgagaa
    13621 gggtcgcacc cgcctccacc gccttcaact gactcatgct ggccagaccc gccgtggcgt
    13681 gggagtgcac ctgaatcggc acaccgaccg ccttgaccag cgcaccgacc agttgggtgg
    13741 ccgcctgcgg cgtcatgatg ccggccatgt ccttgatggc gatcgagtgg cagcccatcg
    13801 cctccagttc gcgggccatg gtgacgaact gttcgatcga gtggaccggg ctgaccgtgt
    13861 agctgatcgc cccctgtgca tgtttgccgg cggcgttgac cgcctcgatc gccacccgca
    13921 gattgcgcag gtcattgagc gcatcgaaga tgcgaaagac atcgatgcca ttgtcggccg
    13981 atttctgcac gaaggcgcgc accacgctgt cggagtagtg gcggtagccg accagattct
    14041 gtccgcgcag caacatctgc aaccggctgt tgggcagtgc ccggcgcagc ttgcgcagcc
    14101 gctcccacgg atcttccttc aaaaagcgca ggcagctgtc gaaggtggca ccgccccaca
    14161 cttcgagcga ccagaagccg atccggtcga gcgccgcgca gaccggcaac atgtcctcgg
    14221 tgcgcagccg ggtggcaatg aggctctgat ggccgtcgcg cagcacgaca tcggtgattt
    14281 gcactcttga catggcgact ccttgatcaa aggccggctt gcgcggcgat caccgccgag
    14341 atcgccgcgg cgatgttctc gtgcggacga cgcagactgt aggcgagcag ctccgggtgc
    14401 gaatcgacga aggtggtgtc gaaacgcccg gcgcgaaagg ccggatgttg cagaatttgc
    14461 aggtagtagg gaatggtggt cttgaccccg gagaggctca ggtcgttgag cgcccgccgc
    14521 gagcgggcga tcagcgcctc ccagtcgagc gcccaggccg tcagcttgac gcacatcgag
    14581 tcgtagtagg gcggaatcac atagccggtg tagatcgccg catcggtgcg cacccccggt
    14641 ccgccgggcg catagtaacg gctcacccgg ccgaagctcg gcagaaagct gttctgcgga
    14701 tcctcggcat tgatgcggaa ctcggcggca aagccgcgaa agctgatgcc atcctgttgc
    14761 atcgacagcg gctcgccggc ggcaatgcga atctgctcct gcacgatgtc gagcccggtg
    14821 atggtctcgg tgatggtgtg ttcgacctgc aggcgggtgt tcatctccat gaagtagaaa
    14881 tcgccctggg catcgagcag gaactccacg gtgccggcat tctggtaacc caccgcgcgc
    14941 gccgcctcga ccgccagcgt gcagacccgc tgccgctgcc gcggcgagag ctgcggtgac
    15001 ggcgcgatct cgatcagctt ctggttgcgc cgctggatcg agcaatcgcg ctcgaacaga
    15061 tgcaccacgt tgccatgggc gtcggccagc acctgcacct cgatgtggcg cggcgcgacg
    15121 atgcacttct cgacgaagac ctccggcttt ccgaacgcct tgctcgcctc cgagaccacc
    15181 cgctcatagt tgcgcagcag ttcggcatcg tcggcacact ggcgaatgcc gcggccaccg
    15241 ccaccgttgg tcgccttcag catcaccggg tagccgatct gcgcggcgat tgttcgcgcc
    15301 tgctcgacgc cggcgaggtt gccctcgctg cccggtgtca ccggcaggcc ggcggcgatc
    15361 atcgccttgc gtgcctcgac cttgtcgccc atgcgccgga tcacctcggc cgaggggcca
    15421 atgaagcgga ttccgcgccg ctggcaggtc tcggccagca gcgggttctc ggacagaaag
    15481 ccatagccgg gatggatcgc atcgcagccc gaggccaccg ccagattgac gatggtgtgg
    15541 gcattcagat agccctgcat cgggtcggcg ccgaccgaat aggcttcatc ggccttcttg
    15601 acatgcagtg catggcgatc ggcatcggtg tagatggcca ccgacttgat ccccatctcg
    15661 gcacaggcgc gggcaatgcg caccgcgatc tcgccccggt tggcaatcag gattttacga
    15721 atcacgccga gctccgtctg ggttgtccgt tactggaatc tgcgtcaatc agaagcaaaa
    15781 gggtgtgcag cttaccctgt ctgcacccat ttcacgatat caagcaaatt gcatcccaac
    15841 aggcgctgca cccgatggaa gcaatcagtt caacagaaga gaccctcgac cgacgaacgg
    15901 ggaaagtgtg aaccctttca ggctgttata cactgtggcc gcccggcctg ccgtaccgtc
    15961 catgcaggcc atgccctgat ccctttgcca ggagtgccgc caatgccttc agttgaacga
    16021 aattgggccg agaaacgtga tttcatccgg atgcggctca acagcgaggt cactctcgaa
    16081 atcgaccagc cgccacgcac actgcgcggt cgctgccgcg acttgagcgg tgccggcctg
    16141 tcggtggaac tggacgaggc gctgccgacc ggcacccact ttaccgccgc ctttgccagc
    16201 atcgacggca gccggctcga cgaaacggcg attcagaccc gtgccgaggt ggcccgcgtc
    16261 gagcgaaccg ccaacggcca cttcctgcat ggctgcctga tgctggagat tctggcctga
    16321 gcgacgccga cggtcggcgg actattcgtc gctctcgtcc agaaagggat ccctggtctg
    16381 ccccttgatc agaaattcac ggttctgcag atagacatcg cggatgaaca ggtagcggtc
    16441 gccactgatc agcttttcgc tcgacagcag gtgcgcccga acgatcaccg ccttgaagcc
    16501 gatcactgcg taacgggtct catcctcgtc gatccgggcg atcggatcga gcatgaagtc
    16561 cggcgccagc ccgacaccgt cgcgcaccgt gctcggcccc agcagcggca gcaccagata
    16621 gggcccgctg gagagacccc agacgccgag gctctggccg aaatcctcgt catgcttgcg
    16681 cagatcgaac cgcgatgcca catcgatgaa gcccagcagg cccacggtcg agttgagcac
    16741 gaagcgcagc ccatcgccgc cagcctgttt gaacttgccc tgcagcaagc cgttgacgat
    16801 cacccggggc tcgctcaggt tgccaaagaa gttgtagagc ccgcggtcgg cccagtcggg
    16861 catcaccacc atgtaaccgc gcgccagtgg cttgagcaga taacgatcga aggtgtcgtt
    16921 gaagacgaac accttgcggt tgaacccctc ccatggatcg gggttgtcgt gctgctgctc
    16981 gccactccag gcgggcggca caaacagcag cgccatcatc atgaaacggc gcactgctct
    17041 gccagacaat ctgctttctc gccacacctt caaaacccgc tctcctcatg ggcgcctggt
    17101 catcacgccg gcgctgccgt tttaatgata cttcaccgcc gcgcccttgc cagaacctcc
    17161 gccaatcttg ccgatgccga ctgcctgtgt gaccattgcc actgtcaggg aaatccgcct
    17221 catgcaggct gtacagcgct gcgccaaccg gcaggacgac ctgccatcat ccgcaagcag
    17281 acctgaccag acacctgacc aatccaccct tcacaataat cacaccattg ataggtaacc
    17341 gtatgagcag acccgtccac gtcatcggcg tcggcatgac ccagttcacc aaacccggcg
    17401 ccagcgaaga ctattcggtc atgggtgcca aggcgatcgc cgacgccctc aaggatgccg
    17461 gcatcgacta ccgcgaagtg cagcaggcct ttgccggcta cgtctacggt gacagctgct
    17521 gtggccacaa ggtggtctac agcgccggac tcaccggcat ccccatcttc aacttcaaca
    17581 gcaactgctc gaccggttcg agcgcgctgg ccgtggcgcg ccaggcgatt gcctacggtg
    17641 ccatcgagtg cgcgctggtg gtcggcttcg agcagatgac cgccggtgcc ctcaagagct
    17701 acttcggcga ccgcagctcg ccgggcgagc gccacggcgc caccatggcc agactccagg
    17761 gcgtcgacat gaacgcgccc ggcgcggcac agatgttcgg cggcgccggg cgcgagcaca
    17821 tgaagaaata tggcaccaag gccgaaaccc tggccaagat ctcggtcaag gcgcgcaaac
    17881 atgccgccaa caacccgctg gccctcttca ataccccgct cagcctcgaa gagatcatgg
    17941 ccgcgccgat ggtgttcgag cccctgaccc gctaccagtg ctgcccaccc acctgcggtg
    18001 ccggcgcggc ggtgctctgc tcacccgaat tcgctgcccg ccatggcctc ggcaatctgg
    18061 tcaccatcaa ggcccaggcc gtcaccaccg acaccgaaag cagcttcggc gacagcatga
    18121 tcaagatggt cggctacgac atggcccgtt cggccgccca gcaggtctac caggcctccg
    18181 gcgtcgatcc gcaggacatc gacgtggtcg aactgcacga ctgcttcacc gccaacgagc
    18241 tgatctccta cgaatcactc ggtctctgcc ccgaaggcgg cgccgaaaag ctgatcgagg
    18301 acaatgacaa cacctatggc ggccggttcg tcgtcaaccc ctccggcgga ctgctctcca
    18361 agggccatcc gctgggagcc accggcctgg cacagtgcta cgaactggtg cagcaattgc
    18421 gcggccgcgc cgaccggcgt caggtcgagg gcgcacgtct ggcactccag cacaacctcg
    18481 gcatcggcgg cacctgtgtg gtgaccctgt acgagcgcgg ctgatcggcc acggccgaag
    18541 ggcccacgca aaaagccgga tcgatctgat ccggcttttt tttcgggtga aaccgcccgg
    18601 caaggcgacg cagaagagga gatcagccag cgctctggcg cgcatccagc cgcagcagtg
    18661 gggaccaggc gactcgctgg gcaaagaaac cgatgccgcc ccacatcgtg gcgaaccaga
    18721 gcggcacccc gccccagggg gcaccgggat agtgccactg gccgctgtgg acgccgacat
    18781 attcgaacag cgcgcccagc agcagcagat agccggcatg gcgccaggcc agagggtcgg
    18841 catcgagcca gcgggtcacc gccagcagtg acgcactggc cagcagcagc caggttgggt
    18901 cgctcagcgc ggtgaaacag agcgcgaaga gccccatcag cagccagcag accgctgttg
    18961 ccggcgccga gcaggcaggt ccgagcaggc gccgggcatg cagcaggtag aagccccaca
    19021 tgaagaactc ataccagggc agtcccagca ggtcggggga agtgaaggcg aagatcccct
    19081 gccgcagcgc catcaggttc atcaccgtga acagcaggca ggagagcccg aacagccagc
    19141 gctcgctgcg ccgggtcggg ccgaaactcc accaccagaa cagcagcagc agcggacact
    19201 tgatccagtt ggcgatcggc agggccagca gggcgactgt gacccagggt tgcagccaca
    19261 gtggccagcg gcgctcactg ctgctcatcg catcaccctt ttctcaggcc gcgaacagtg
    19321 tgtcggccgc acaataatcg cgtccctgcg cctcggccac cgcctgacag gtgagccggc
    19381 cctgatggac attgagcccc gcccgcaacc cggggctgcg cagcaacgcg gcagacagcc
    19441 ccgcatcggc cagctcgagc acatagggca gcgtggcgtt gttgagcgcc agcgaggcgg
    19501 tgcgggccgc cgcgctcggc atgttggcga cgcagtagtg gacgacaccc tgttcgacat
    19561 aggtcggcgc acggtgggtg gtcggtcggg agctttcgaa acaaccgccc tgatcgatcg
    19621 agacatcgac cagcaccgca ccggggttga ggctgcccag cagcgtccgg ctgatcagtc
    19681 gtggtgcgcg agcgcccgga atcagcaccg caccgatgat cagatcggcg gtgaccgcct
    19741 cgcgctcgat gttgtgggtg gtggcgtaac aggtgtcgag gcggccgcca aactgctgtt
    19801 cgagctgccg caaccgttcg atcgagcggt cgagcagcgt cacatcggcc cccatgccga
    19861 gggcgatcag cgctgcgctg ctgccgacca cgccgccgcc gagaatcacc accttcgccg
    19921 gcttgacgcc gggaacgccg gccagcagca gcccccgccc cccctgcgac ttctccagag
    19981 cgtgcgcgcc ggcctggatc gccagccggc cggccacctc cgacatcggt gtcagcagcg
    20041 gcagttgccc gccggcactg gtgacggtct cgtaggcgat gcaactggcg ccgctgtcga
    20101 gcagggcctg ggtcagggtc ggatcgggcg ccagatgcag gtaggtgaag agcagctgcc
    20161 cagcgcgcag cagaccatat tcgcccggct gcggctcctt gaccttgacg atcagcgccg
    20221 cacgcgccca gatctcggca gcgctgtcga tcagcgtggc accggcgcgc tgatagtcgt
    20281 catcgccgaa accgctgccc agtccggcac cacgctcgac cagcagccga tgcccatgca
    20341 gcgccaactc gtgcacggca tggggcgtca gcccgacgcg gtactcctcg gtcttgatct
    20401 ctctgggaac accaatgatc acgtggtcac ctcaccgcga gaaaatgtct gtccagccgg
    20461 cagcaaaatg ggggccagga cggcggtgcg ccactcaaag cggctcgccg cgcacatccc
    20521 gatcgacgat ccggtacttc tgctcgcgcg cccagttctt gaacctgcgg acatcgagct
    20581 cgctgacgaa gcggagcacc gcatagcggg tcatgtcctg caggatcggc ccggcagcga
    20641 tgatgcgggc attgcgcgga tgaaaatgat cgagcagcca ctcgatcacc ttcacatcgt
    20701 gatggtgagg atagtagacg ctgagcagga tgatcggttg ctcaacgggc gtgcggcgcg
    20761 gttgcatcat gttcgattcc atgaaacctg aaacgggcgc tgaagggtgc gctgctgcgc
    20821 ggacggacgg cacggcccgg ccgctcgaag cgccagggcg gcccggattg accacggcag
    20881 gccggcgcca gcagatagac cgcggcggcc accgccgcac cgccagaatg cggagtgacc
    20941 cgcagcgacc gacgacggta gcgggcgcct tcataacgat caagccgctg ccattcgtcg
    21001 ggagcaagac cacggtagag cgttccgggc gtcactgcac ctcgccgtgg ccgcagacaa
    21061 ggatagggcg tgccacgcag ccgaaccacg gcatggtctg gcagccaggc ccgagcggca
    21121 gcggggaggc gcccgatcag cgcggtgatc agcgcagccc gccgcagggt gccataagcg
    21181 aacagcagcg tctccgggtt gtcggtcacc acgctcacac cggacaactg acgccggtgc
    21241 ccttcagccc gcagtagccg cccggattct tgtcgaggta ctgctgatga tagggctcgg
    21301 cgtagtagaa ggggccagcc agcgcgatct cggtggtgat gcgaccaagg ccggcgccct
    21361 gcagcgcctg ctggaactga tcgcgcgagg ccagtgtggc ggccaactgc gcatcgctga
    21421 agcagtagat ggccgaacgg tactgggtgc cgaggtcatt gccctgccgc attccttgcg
    21481 tggggtcatg ccgctcccag aacagcgtca gcaactgcgg atagctcacc cgctcgggat
    21541 cgaacagcac ccgcaccacc tcgctgtggc cggtgcggcc ggagcagacc tcctcatagg
    21601 tcggattggg ggtgaagccg ccggcgtagc ccactgccgt gccgtagaca cccggcgtct
    21661 gccagaacag ccgttcggcg ccccagaagc agcccatgcc gaacagcacc tgctgcaaac
    21721 ccgccggaaa gggcggctgc aacggggtct tcagaacggt atgcagtgcc gggaccggca
    21781 tcgcctgcga ccgtcccggc agggcctgct cggcgctgat cggttccagt ttgctgcggt
    21841 ttgcagggga catggttcac tccgctgtca caggtttttc agtttgcgtt cgacgctttc
    21901 gaccttctgc tgcatgctct ggtcgcggtc ggtgcgggtg ccgacccgca aggtggtgtg
    21961 gatgcgcggc gcacccatcg cgtggacgcg ctggtggcac tgcttcacca cggcgaagac
    22021 ctcatcccag tcaccttcga tgatggtgcc atagggatgg agctgatggc tgaggccagc
    22081 cgcagcgatg atctgctggc aggccgccac atggtcggag accgagacgc cgacaccgat
    22141 cggcaccacg cagagatcga tcatcacctt catcggcccg ccctcacctg ctcgcctcat
    22201 catgaagcag gatgatgccg ccacggcgcc atgcaggcaa tatgaacctc tgtgtgctca
    22261 aacccgatcg agccacacct gcaactgctc gccatcgaac ggccagccca gttctgcgcc
    22321 atcggccaga cgcctgagca ccggaatggt ggtgccatag cgctgcagca gcgcctcatc
    22381 gtcggcgatt tccacctcgg tcagcatctc gccacgcagc gtcagcagcg gcagcgcgct
    22441 ctgcagcgcc agttcgcaga ggtggcagcc cgaggtggag tagagtacaa aaccgctcat
    22501 gccgccctgc cgagaaagtc gaggatggcc tgcgccactt cgggcgccga atcttccagg
    22561 tgcagatggt ggccaccggg caggatcagc ttttgcaggt tgcggtgcac cgcggtgcgc
    22621 cgcgcgatga agtcggcgct ccagggccag ccctgttcgc ccagaatcag cagtgtcggt
    22681 gcctcgacgg cctggatgaa ggcctgcacc tcctcctcgg tcaaccgtgc cggatcgggg
    22741 tagcgcagcc gcgaatcgga actccaggtc aagccgccat tgcagggcat gacaccacgg
    22801 tcgagcagcg tgcgtgcggc cgcctcggtg atgttgccaa cggtcttcat gcgcggcagc
    22861 agcatcgcct cgaaattttc atacagcggc ggtgtgcggt tttccagccc atgcagccgc
    22921 tccagcgcct gccgcgtttc agcgacgatg cgttcgctct tgaggccggg atggccgatg
    22981 gcatcgatca gcaccagccg gtcgatgcgc tcgggaaagg ccgccgccat gtagaggccc
    23041 aggtgcgccc ccatcgagtg acccaccagc gtgaactgcg cccagcccag ttgatcggcg
    23101 acggcaaagg cgtcgttcat ctgcagaaac cagctgtagg gcacccccgg cggcaggtgg
    23161 tcggagcggc catggccggc aaagtcgatc gcgcaggtct gatacccctc cagcagcggc
    23221 gccagcagat cgaaggtggc ggcattgtcg agccagccat gcagcgccag cagtggcctc
    23281 ccctgcggat tcggccagaa acgggccgca atggtgcggc caaagacggt aaaacttttc
    23341 tcggtacaga ccaacgacat ccagatgact cctgactgct gagacttcaa ggcgggcaaa
    23401 cccaaccatc aatcaaatca gtatgttacc tcactggagc gcgatttcgc accggcccgg
    23461 cagcgcttga tccgattttg ttcgcgctgc gccggcgaag cgccgatccc cgtctatact
    23521 caaaatgtgt gaaaccgttc tcactttcgc ggcccggatc gatgacctcc ctgaatcaac
    23581 tgctcgacaa cctgcgcacc aacgaaacac tcgcccgcaa gatcttcgag atcgagagcg
    23641 aggtgctggc gatctacaac agccgcaact tcttcgatcg gctgctggcg ctgatccagg
    23701 agaagttcgg cgtgcccgag gcctgggtgg cgctgatcga tgagccgatc accgcgccgc
    23761 tgattcacca cctctcccac tgcgaccagt tgacccgcag tctggtcccg ctgtcggcag
    23821 ccggcttgcg gcaactctct ggccactttc ccgatgcctg gctggtcagc cactgcgcgg
    23881 agcggcacaa ctggctgatt cccccctatc tgcaagggat ggtgcggtcg atggcggtga
    23941 cgccgttgca gctcgaaggc cggctgatcg gcgcgctggt gcagggcgat cgcaaaccaa
    24001 accgctggga tccgtcgatg gacacctttt ttctgtcgca actgacggtg aaggtgtcgc
    24061 tctatctggc caacctggcc tcgcgcgagc agctcgaata cctggcgacc cgcgatgcgc
    24121 tcaccggcct gtacaaccgc cgcgagttcg atcaggcgct ggagcgggag atcagccgtg
    24181 cccagcgcag tggtgcaccg ctgtgtgcca tcttcatcga ctgcaacgac ttcaagcaga
    24241 tcaacgacac ccatggccac gatgccggcg atgccgtgct gcgccatctg gcccagggac
    24301 tggagaagct gatccgccgg gtcgactgcg cctttcgcta cgccggtgac gaattcgtgc
    24361 tgctgctgcc cggacagcga caggagcagg cacagtgggt ggcacagcgg ctcgaccact
    24421 actttcgcgc ccacccctgt ccattccgtg gccagtcgat cccctacagc atcagctgcg
    24481 gtgtcgccag caccgagcag agcggcatcg acagcggcac ggcactgctg aagcaggccg
    24541 acatgcagct ctatcaggcc aaggggcgca agcatgaggc agtcgaccac cgcctctcca
    24601 cccgcatcca tgccctctgg caccgcttac agaaggtgtt cgacaccgta taatcgcccg
    24661 cttcatcgac cgggagcgag catggcccat gcggcaacga atcggactct ggctggggcc
    24721 cttgctgctg ttggcgatcc tgttgctgcc ccggcccgaa gggctctcca gcgaagcgca
    24781 gcgggtcggt gccgtggtgg cgatgatggc catctggtgg ctgctcgatg ccgtaccggt
    24841 cgccatcacc gcgctgctgc cgatcctgct ctatccgctg ctcggggtga tgaaatcctc
    24901 cgaggtgacc cccagctacg ccaaccacct gatctacctc tttctgagcg gtttcttgct
    24961 ggcactggcg atcgaaaagt gggggctgca ccaccgcatc gccctgcgca cactggcagt
    25021 gatggggcat tcgccggagc gggttctgct cggtttcatg ctggtcaccg cgctgctcag
    25081 catgtggatg agcgccacca tcaccgcaat gatgatgctg acggtggcgc tggcgctgct
    25141 gcacgatgag ttcggcagcg cgcccagccc ctacaccgaa cgcggccgcg atcagcgcgc
    25201 cttcgccacc agtctgacac tcggcatcgc ctatgccgcc tcgatcggcg gcgcagtgac
    25261 gatcgtcggc tcgccaccca acgccattct ggccggcatt ctggcgcagc agcacgaaat
    25321 gagcctcagc ctgatcggct ggatgctgat gacgctgccg gtcgcgctgg tcatgctgtt
    25381 cggtgtctgg ttctacctct cccgcatcgc ctacccgacc cgtttcaacc acctgcccgg
    25441 cggcaccgcg ctgttccaca aggcgcacca ggatctgggc gacttcagcc gcgccgagcg
    25501 gcggctcatc gcagtctttg cgctggtggt ggtcggctgg attgtgcgcg ccctcaacca
    25561 gagcgagttt ttcgactggg tgcaggattc gaccatcgcg ctggccggtg ctgtcgccct
    25621 ctttgtcatc ccctccggcc gtcgacccgg cgagccgctg ctcgactgga agagcgccaa
    25681 gcaactgccg tgggagatca ttctgctgtt cggcggcggt ttcgccatcg cccagggctt
    25741 tcaggagacc ggcctgaccg agtggctggc ggggcaactg ctgaacctgc ccgacatcgc
    25801 cccgttctgg atcctgctga tcaccaccct gttcgcgctg gccctgaccg aggtcacctc
    25861 caacatctcc acggccgcgc tgatcctgcc gctggtcggc gccatcgcag tgatcttcga
    25921 cctgccgacc caactgctga tgagcagcgc ggccatcgcc tgctcctatg ccttcatgct
    25981 gccgaccgcg acgccggcca atgccattgc ctatggctcc ggttacttct cgatcgggca
    26041 gatggcgcga accggcctgc cgatcaacct gctcggcgcc ctgctgatca gcggttacct
    26101 ctacctgttc atggatgcgt tcgcgccgct gctgttcagc agtgccagca acggctgagc
    26161 agcggccatg cgtgatgccg accggccctt tgcgctgccc catgccgaga tcgactggtc
    26221 ggcggacgat gaacccttct catcccgctt cggtgaccac tatgccagcc gcgccggcgc
    26281 cctggccgaa agccggctgg tctttctcga tgccaaccgg ctgggcgagc gctggtcagg
    26341 attgcgcagc ggtgagcgct tcacactcgg tgagaccggc ttcggcaccg gcagcaactt
    26401 tctgctcgcc tgggagctct ggcagcggct ggcgccggca gcggcccggt tgcactatct
    26461 ggcggtcgag gcccatccac tgcacccgga cgatctggcc cgcgcactga cccgccatcc
    26521 gccgctcacc gcactgagcg agccgttgct cgcggcctat ccggccgcca tccccggcat
    26581 ccaccgcctc gaactgcatg ccggcaccag ccctgtgacc ctcgatctgc tgttcggcga
    26641 cgccgaggcg atgctggcgc agctctgcac ccgacagggc ttcgccatcg atggctggtt
    26701 cctcgacggc tttgccccgc agcgcaaccc cggcatgtgg cacgacgggc tctaccggca
    26761 actggcccgg ctgtcgcgtc ccggcaccac cttcggcacc tacaccgctg ccggtgcagt
    26821 gcgccgcggc ctgagcgacg ccggtttcag ggtggagcgc gccagcggtt ttggccacaa
    26881 gcgtgaacgg ctgctcggcc atttcgtggc agcgaccgca ccggtcgtcg agccaggtcc
    26941 tccatggagc tggctgccac cggcccgcca tgccgccacg gcctctgcac cggtggccgt
    27001 gatcggcgcc ggcatcgccg gcagcagcac cgcgcaggca ctggcccggc gcggcatcga
    27061 ggtcgcactg ttcgacagcg agaacggcgc cggccgcgcc gcctcgggca atccgcaggc
    27121 ggtgctctac agcaagctct cggcccacgc cacgccacag agccgctttg cgctgcacag
    27181 ctacctccac gcactgcggt tctaccaggc actggagcag cagcgcagcg ccgaacaggc
    27241 gacgctgctg catggctgcg gcctgctgca actggcgacg caggcggcca cccgccagcg
    27301 gctgctcgac atcgcccacc gttccggcct gccgacgacg ctggtgcgct gggtcgacgc
    27361 agcagaggcc agtcagctcg ccggcgtggc gctggcacag ggcgggctgc tctttccgac
    27421 cggcggctgg ctcaccccga ccgcgctctg ccagcagttg agcagccatc cgcgcatccg
    27481 ttgtcacttc ggcaccgcag tgacgcaact gcgccacgac gccgagcatg gcagctggca
    27541 gttgctggat ggtgacggtc actgcctgct gcgcacagcg actgtggtga tcagcgccgg
    27601 cgccgcctcg acccgcttcg aaccgctggc agcgctgccg atcaagccga tcggcgggca
    27661 ggtgagccaa ctgcccgcct ccagcgccag ccggtcactg caactggtgc tgtgtggtga
    27721 aggctatctg gcaccggcgg accaggcggc gcacaccttt ggcgccagct atcggctgca
    27781 cgatgacgac ggcctgccaa gcatcgacga ccatcggcgc aatctggcga tgctgcagca
    27841 ggaattcccg accttccacc agcagctggc agacgaacag ctcgatccag cccgccttgc
    27901 tggacgctac ggcattcgtg ccaccacccc agaccgattg ccgctggtcg ggccggtgac
    27961 agcgggcgcg cagccgatcc gccacctgcc gggcctctac atcaatgccg gccatggctc
    28021 gcgcggattc gtccaggcgc cgctgtgcgc cgaactgatc gccgcgctgg tgagcggtga
    28081 cagcctgccg gtcgagagca cagtgatgac ggcactcaac ccggcacgct ttgccgttcg
    28141 tgcgctcaag caccgcaaac agcagtcgta atcggcaccg gttttcggta aagttcaccc
    28201 cggccgatga tccggcgacc cggctcgaag cgcccgcttc gaccaacaag atcaataaca
    28261 tcaacgacca gaataaaatc gaggaacaac cgatgagcga acctgcctac ctgaacgatc
    28321 cgatggccgc agctggctgc ggcatgatcg tggccttcca tgccaacaaa aaccccaacc
    28381 gtgtcgcgat catggctgat cagggaccca cccgcagcta cggcgaactc aatgcccgca
    28441 tcaaccagat cgcccatctg atgcgcgcca acggcatgaa ggccggtgat gcagtgtcac
    28501 tgatctgctc caaccgcgcc gaattcgtcg aagcgctgat ggcctgcttc cgcaccggaa
    28561 tgcgcgtatc gccggtcaac tggcacctga ctgccccgga gatggcctac atcgtcaacg
    28621 actcggaatc caaggtgctg tttggcgatg cccgcttcgc cgacaccatg gccaaggtgc
    28681 tcaaggagag ccccgacgtc aaactggcgc tgtcgatcgg cggcgacatt cccggcttcc
    28741 agaacctgga gagcgccatc aaggagcaac cgaccagcaa catcgacgcg ccggtccgcg
    28801 gcttccagat gctctacacc tccggcacca ccggttatcc caagggggtc taccgcgagg
    28861 ccgcctcttc gccctcggcc atcacccagt acgccaacta ccagccggcg accgacgtca
    28921 ccctctgcac cgggccgctc taccatgccg caccgctcgg catcgacctg ctggccgcgc
    28981 tcggcaacgg cctcggcgtc tacctgatgg acaagtggga tcctgaacga accctgcaac
    29041 tgatcgaaaa acacaagatc acccacagcc acatggtccc gaccatgttc caccgaatgc
    29101 tggccctgcc cgaagaggtg cgcaagaagt acgacctctc gtcgctgaag ttcatcattc
    29161 atggtgccgc gccctgtccg gtgcacgtca agcagcagat gctcgactgg gtcggcgaca
    29221 tcatctatga atactacggc gccaccgaag gggtcggcac caccatcacc ccggccgaat
    29281 ggagaaagaa accgggcgcc gtgggtctgg caccgcaggg gctgatcatc gtcagcgaag
    29341 agaccgacca ggagctgcca ccgggcgaga tcggcatcat ctggattccg gtgccccccg
    29401 agggcggttt caaatacttc aaggccgaag aaaagaccca aaaggcctac cgcaatggca
    29461 cccactactc gatgcatgac atgggctggg tcgacgaaga gggctggctc tacctctccg
    29521 accgccgcgc cgatctgatc ctcgccggtg gcgtcaacat ctaccccgcc gaggtcgatg
    29581 ccgcgctgct caagcatcct gccgtcagcg atgcctgcac catcggcgtg cccaaccccg
    29641 actggggtca ggaggtgatg gcggtgatcg aactgaaaga gggcgtcaaa ccctctgaag
    29701 aactgaagca ggatctgatc agcttctgcc agcagaacct ggccaagttc aagtgcccgc
    29761 gcaagatcga gttcatggac gcactgccgc gcagcgatgc cggcaagatc cagcggcgca
    29821 aggtgcggga catctacatc ccggccgcct gagcccgctg cgggtcagac ctgcccgtct
    29881 gatccgcatt gaccgatcga tgaaaggccc tggcctcaaa gccagggcct ttctgttggc
    29941 gtgaagattc aaaggtcgga tcacagggca tgccgctttc atttatactt cggccatctc
    30001 acccacatgg ctaccgccaa caccggccac cgcacccatg tcctccagcg caatcacccc
    30061 ctccaccctg ccggcgccac gcagtgccac accgcagtgg ccggtgcgtg cagcggaacg
    30121 caccagtttc gccacgcggg aggccgtcgc ggctaaggac agcgtggaaa ccctgcgccg
    30181 ccccgtctgg cgcgagtcga gcccgcgcgg cggtaccaat ctcgacccat ggcaacgcac
    30241 gctgaccgca caggcgcgcg ccgcactgcg tgcctatggc gagtgggcta cgccaccggt
    30301 aaccgaaacg cctgaagtca ccctggtggg tgtcgacctc tacgtgtgac ccttcagcgc
    30361 gtcggcatcc tgcacgactc cacctcctgc ttcgccgagc aggccgaagc gctggccatg
    30421 cgccaccagt tgccgctgat cgcgatcgaa caggccgatt ctgccgcgct gctgttgatt
    30481 ctcgatcagg atggactggc cctgcacagc atggacgcag cgggcgaaca gccgctgcgg
    30541 atcgatttcg gccatggccg cctcgattac cggcgtcgtc acggtggcgg cacccaggaa
    30601 cccctggccc gggcagtggg tctgcgtggc ggttttcgtc cgcaactgct cgatgccacc
    30661 ggcggtttcg gcaccgatgc ctttgtgctg gccagcctcg gctgccaggt gaccctctgc
    30721 gagcgctcgc cggtgatggc ggcactgctg gccgatgcac tggcacgcgc cggccagaag
    30781 gcggagttgg ccgccatcgt cacccgcatc accctgcggc aatgcgacgc ccgcgacgag
    30841 ttggccagcg gacgacagca cgatgtcatc tgcctcgacc cgatgtttcc accgcgccgc
    30901 aagtcagcgc tgccacgcag gacgatggtg ctgttgcagc agttggtggg gcatgacgag
    30961 gatgccgacg cactgctgcc gctggcgctg gagcgggcgc gctatcgggt ggtggtcaag
    31021 cggccgcgcc aggcgccacc gctggcgggc ctgaccgcgc accacagcct cgagggctcc
    31081 agttgccgct tcgatgtcta cacgctgcgc aagctgccgt cgaagcagga ggcggcagag
    31141 gcggcttcaa cctcctgaag agagaccccg atcggcccgc ttgcgccagg agatctgttc
    31201 gcgcagatag ctgggctggg cggcttcggg cggcagcagt tcaccgcgcg ccagcgccgg
    31261 cagtgccagc gccaccacgt cggccgcgcc gggcagcagt ccggcgtcga tatgggccgg
    31321 ccatccagcg agcgcttcga ccagcggctg tggctggctc cagccgttgc cgaccccgac
    31381 cgtgtcggcc agatcgagtc cggccggcag cggaatctgc gctgccggcc ccagccactc
    31441 ctccagcagc ggctgcggcc gctgcgcttg caactgatag ccgcaccagt aaatctcgcc
    31501 catgcgcgca tcgacggcgg tcaacacccg ctgctgcgga tgacgcagcg cggccgcatg
    31561 ggccagcgcc gccagactcg acaccggcag cacgccgatc tccgcgccca acgccagccc
    31621 ctgcgcgacg ccacaggcaa tgcgcagtcc ggtgaaggag ccgggtccgg cgccgaaggc
    31681 gatggcatcg agctcgccaa agcgggcatc gtgctcggcc atcaaggctt cgatcaggcc
    31741 cagcagctgg cggttgtgtt cacgcggcag atcgacctgg cgctcgctga gcgcgccctc
    31801 ccaccacagg gcaaccgagc agcgttcact ggaggtctca agggcaagca gtctggccat
    31861 ggtcgggcat ttcgctgtcg gcggcaggga ggggtgagca gagcgcttcg aggaactcga
    31921 ccgcctgcgc ttcgtagtcg gtcaccggca tcggcggcag cgactggcgg aagattctgc
    31981 catagctgcg gctgcgcaat cgagggtcac cgatcaccag cacgccccgg tcggtcacgc
    32041 cgcgaatcag ccgccccacc ccctgcttga ggctcaccac cgcctgcggc aactggtagt
    32101 cgttgaacgg gttgccgccc tggcgacgca tcgcatcgac ccgcgcctga gtcaccggat
    32161 cgtccgggct ggcaaagggc agcttgtcga tcgccaccag ccgcagcgat tgaccgggga
    32221 tgtcaacccc ctgccagaag ctgctggccc ccagcagcag ggcatcatca cgctcgatga
    32281 accgttcgag catggcgcgc cgcgacagct cgccctgcac cagcagcggc aggtcaatct
    32341 gttgccgcag cagcgtcgct gcctcgcgca gcgcccgatg gctggtgaag agcagaaagg
    32401 ccccgccccg gctggcgcgc agcagcggca gcagccgctc gatccagcag tgggtgaagt
    32461 cgggctggtt gggctcgggc atctgcgccg gcagatagag cagtgcctga cgcgaatagt
    32521 cgaacgggct gggcagcacg cagccgcgcg gtggccgggg cagccggtcg agccccaact
    32581 gggcgatgaa atggtcgaac ttctgaccga cggccaaggt ggccgaggtg aagatccagg
    32641 ccgcacggta gccggtgagc gcctgacgga agggttcggc caccgacagc ggcgtgcggt
    32701 tgaaggcaaa gccctgctcg ctggtctcgt accagcgcac cgactcctgc gcctgctcgc
    32761 tcagcagcgt ggccagaatg gtcgacagcc ggccggcgcg ctcgcaacag agggccagtt
    32821 cgcggccccg tccctgcacc agttcgagcc gctgccgcag ttgctcgagc atctcgtgca
    32881 actggtcggc ctgggcgctg aactcggcgc ggtcgagcag gcggttccac ggcgcgcgct
    32941 gctcgctcgg ccccagcgtc cgccgcagcc gccgcgcagc ctgttccagc ccggccgcca
    33001 gctgccgcaa cggcaggtca tcggccgcct ccttgagcgc cgcaccctcg ctgtcgcgcg
    33061 ccagttcgat caactgccgg ctcgacagcc gctcgccgaa gaactggccg gcaatctccg
    33121 gcagcagatg ggcctcgtcg atgatcaggg catgggcacc cggcagcagt tcgccgacgc
    33181 cttcctcctt gagcaccatg tcggcaaaca gcaggtggtg gttgatcacc accagttcgg
    33241 ccttcagcgc ctcgcgccgc gcctccatca gcggacagcg gctgaaatcg ggacactcct
    33301 gccccaggca attgtcggca ctgctggtga tgcgcggcca gagcggatgg gcctcgtcga
    33361 gcgcaggaaa gtcgcccaga tcaccactgc cgccaccgct gtgccactgc gcaagttcgc
    33421 ccagcagttg ccgatcttcg tgctcgaaca gtgtcacgct ctcgagcgtc aggcccagcc
    33481 ggtgcggaca gaggtagttg ccacgcccct tcagcagcgc gctcgacaga tcgagtccca
    33541 gcacccgcag cagcgccggc aggtcgcgga aaaagagctg ctgttgcaga ttcttggtgc
    33601 cggtggagat gatcacccgc ttgccgctga tgacggccgg cagcaggtag gcgaaggtct
    33661 tgccggtgcc ggtgccggcc tcgatcacgg cgatgtcgcg cgcttcgagg gtgtcggcca
    33721 ccagcgccgc catcgccatc tgcgactggc gcggcatgaa gcccggcagt tgtgcggcaa
    33781 agggaccggc ccgatcgagc agcgcggcga ccgggtgtat ggcgggcggc ccggttgcgg
    33841 cgtcgacggc cggcggttgg ctcaagtcgg gctccagacc cgcgtggcca gcgccttgat
    33901 ccgggccacc gagagggcgc cgcgcttctg ccgcccggca ctgccgtcgc cgaccgccgc
    33961 gctggccgct tcccgatcga gctgatagat cgccgtcggc tggtagctgg catccatggt
    34021 caccagcagc agccgatccc agccaccctc gaggctgatc tggccgatgc gctgcccgcc
    34081 acgctcctga ttgaagatca cccggctctt gatctgatac ttgagctgcg ccgcatcgat
    34141 ggcatccacc ccctgctcgg cgttgtggag cagggtcaga ccgagcaggc gcgccgcgtc
    34201 atggcgggcc agctcgccgc tcaccggcaa cacctgccct gttgcgcgcc ggtattcggc
    34261 ggcaatgcgg cgggtctctt cgatcagtct gtcgacctgg taaagcatgg ggctctctgt
    34321 gacctggttg ctgggcgatg gtagtatccc gccatgcgtg acgcaaacag tccagacccc
    34381 gctctgccac gcgcccagat caccggcgtg attctggccg gcggcgccgg tcagcgattg
    34441 ggggggtgcg acaagggact ccaccccctt gcaggccgcc cgctgatcgc ccatgtgcgc
    34501 gagcggctgg cgccgcaggt gggcacactg ctgatctgct gcaaccgcca ccacgaccgc
    34561 tacgcccagt tcggcgatcg actgatcagc gactcgcaac caggccatgc cgggccactg
    34621 catggcatcg ctgccgcgct cgcggtctgc accaccccct atctcgccat cgcgccctgc
    34681 gacacgccgg ccctgccagc gcactgggtg gcgcggctgg gccgacaact gctcgagcac
    34741 aatgccgagg tggcagtggt gcatgatggc gaacggctgc aatcgctctg cgcgctctgg
    34801 cgcgtcgagc tggccggcgc ggtggccgac catctggccg ccggcggcag tgcgccacgc
    34861 gaattctatc gccgctgtcg cagccggtgg gtcgatttct cggccgagca tgccgccttc
    34921 gtcaacctca accgtgcgca ggagatcgcc gcctatggca acctgatcgc gcagggccac
    34981 ggcatcacgg cttgtagagc aggtgatgga tgaactcggc ctcgtgccac tgtcgcagca
    35041 actggtaact gctctgtttg gcgaagatct ggaagtcgcg ctccgaaccg gggtcggtgg
    35101 cgaccacttc gaggtaactg tcggccggca gccgattgag cgcctgcttg gccttcagca
    35161 gcggcagcgg gcagttgagc ccggtgacgt ccagacgctg atcgatccgt tgcgtcatcg
    35221 gctgcaccct gtatcagccc attgaaatcg atgcatccgg gttgccctca tcgaatcaga
    35281 cagaccatca cgaaaccggc agcgaccatg ccggtgagcg ccacaaagaa cagcccgtcg
    35341 gcgaccattt cgagccgctg cgcgtggcga ctctccctgt ggcgcagcgc aaagaagatc
    35401 agatagcagc tcagcagaaa cagcagtgcc gagaaggcga gcatctcatc ggccagactc
    35461 tccatccttg agctgccggc caggccatgc agcagggtca ctccggtgat gcacaggccc
    35521 gccagagtgc ccgacagtga cagcaggtgg agcagctctt cgcgggccca gctcttcttt
    35581 ggcgagaaaa acaccatggc tatgatcctg aatgtgaaga aactttttcg ttttcaaccg
    35641 gctgcacagc agaggcatac ccccatgccg gcagcagccg ctgctcgatg cccagatggg
    35701 tgagaatgcg ggccaccacg aagtcgatca gatcctcgat gcgttgcgga cggtggtaga
    35761 aaccgggact ggccggcagc agcaacgccc ccatgcgggt cagccgcagc atctgctcca
    35821 gatgaatctc cgagtagggc atctcgcgcg gcaccacgat cagccggcgc cgctccttca
    35881 atgccacatc ggcggcccgc tcgatcaggt tgtccgacag cccctgcgca atcgccgcca
    35941 gcgtgcccat gctgcagggg cagatcacca tgccggcgct gggggccgat cccgacgccg
    36001 gtggcgccag ccaatcctcg cgcccgaagg cggcgatctg tcccggcgcg gccctgaacc
    36061 gctcggtcaa cgctgcctgc aaagcagctg gattggccgg caggctccaa tcggtctcga
    36121 tctcgatcac ccgatgcgcc gccttggaga gcagcaactg gatcgggcgg tcggccgcca
    36181 ccagcagttc gatcagccgc agcccatact gcacccccga agcaccggtc agcgccacca
    36241 ccaccggcgc cccgaccagc atcgacggcg aactcatggc gcagccaacc ccagcgcctc
    36301 gaccagccgc cgtggcaagc cgtcgaagcc gccgttgctc atgatcacca gatgctgcca
    36361 gcggccacgt tcggccacca gccgttcgat catcccggtc acgctggtga agcggtgcac
    36421 cggcactgtc ggatcggctg cgggcaccca gtccatcccc tccggctgat accagaaaac
    36481 cgcatcggcc gacgaccagg cggccggcaa ctgcctgcgg tgcacgccac tgcgcatggt
    36541 gttggagcgc ggctcgatca gcagcagcag cgactgattg ccgatgcggc gccgcagccc
    36601 gcgcagcgtg gtggcaatgg cggtgggatg gtgggcgaag tcgtcataga cagtgatgcc
    36661 atcgccgctg ccgatcacct ccagccgccg cttcactccc tgaaaccggc ccagcgcggc
    36721 cgcggccacc accggatcga cccccacgtc ggcggcagcg gccatcgcca tcaccgcgtt
    36781 ggccacattg tgacgcccga gcagcgacca ctgcacctcg gcccgttgca cgccatcgcg
    36841 gccgacgaca aaggccgagc catcctcctt caccagtcgc gcccaccagc cgctctgcgg
    36901 aatggtctcg ccccgttcga gacagccgag ccacgcctgc cgccgactcc agcagccctc
    36961 gctcaacacc tcctgcaggg cgctgtctgc cgtcggggcg atgatcaccc cttcgcccgg
    37021 cacggtgcgc accagatggt ggaattgccg ctgaatcgcc gccagatcgg gaaagatgtc
    37081 ggcatggtca tactcgaggt tgttgagcag cagggtgcgc ggccggtagt ggatgaactt
    37141 ggagcgcttg tcgaagaagg cggtgtcata ctcatcggcc tcgacgacga agtggctgcc
    37201 cgaaccgatc cgtgccgaac agccgaagtc gcgcgccacc ccgccgatca gaaagcccgg
    37261 atcatggccg gcgcactcca gtatccaggc caccatgctg gtggtgctgg tcttgccatg
    37321 ggtgccggcc accgcgatca catggcgccg gttgagcaga tggcgcgcca gccactccgg
    37381 acccgagcag tagtcgaggc cacggttgag caccgcctcg acagcgggat tgccgcgcga
    37441 catcgcattg ccgatcacca ccagatcggg ccgggcatcg agcggagccg ggtcatagcc
    37501 ctgatgcagc gcaatgcccc gcgcggcgag ctgatcgctc atcggcggat agacattggc
    37561 atccgaaccg gtgacccggt gccccagttc gctggcgaga atggccagac tgcccatgaa
    37621 ggtgccgcag atgccgagaa tgtgcagatg catgagatca ggatgactcg attgaaggag
    37681 caggcgcgga atcggtgcga ccgccagcgc cggctggcgc ggacgcggtt attatagtga
    37741 cattcgccgg cgccatggtc gaccccgatg gaacggcgcg tgcatcggca atgcctccag
    37801 caacctcgac aagggacgca ccatgaccca gatgaatgcc ttctatgccc aatccggcgg
    37861 tgtcaccgca gtgatcaacg cctcggcctg cggcgtgatc gaatgcgcca gccggcacag
    37921 cgaccgcatc ggcaaggtct acgccggccg caacggcatt ctcggcgccc tgcatgaaga
    37981 gctgatcgac accagccagg agtcggccag cgccatcgcc gcactgcgcc acaccccggc
    38041 cggcgccttc ggttcctgtc gctacaagct gaaggggttg caggagaacc gcgccgagta
    38101 tgcgcggctg atcgaggtct tccaggccca ccggatcggc tacttcttct acaacggcgg
    38161 tggcgactcg caggacaccg cgcacaaggt gtcgcagatc agcgtcgaga tgggccatcc
    38221 gatcgtctgt gtcggcattc ccaagaccgt cgacaatgac ctgccgatca ccgacaactc
    38281 gcccggcttc ggctcggtgg ccaaatatgt ggcggtctcg accagggagg ccggcctcga
    38341 tgtcgcctcg atgagcgaaa gctcgaccaa ggtgttcatt ctcgaagtga tgggccgcca
    38401 cgccggctgg attgccgccg ccgcgggcct ggcggccgac aagaccggcg acccgcccca
    38461 tctgatcatc tttccggaga tcgccttcga cgaggcgcgc tttctggcca aggtcgatgc
    38521 cagcgtcaaa cagcatggct actgcgtcat cgtggtctcg gagggggcgc accatgccga
    38581 cggccgcttt ctgagcgatg ctggcagccg ggatgccttc ggccatctcc aactgggcgg
    38641 tgtggcaccg accctggcgg cactggtcaa gcgcgagctg aactacaaat accactgggc
    38701 ggtggccgac tacctgcagc gggcggcgcg ccacatcgcc tcgaagaccg atgtcgacca
    38761 ggcctatgcg gtcggcgagg ccgccgtgga gttcgccctg gccggccgca atgcggtgat
    38821 gccgaccatc gtgcgtcagc ccggcacggt ctaccgctgg agcatcggcg ccgcgccgct
    38881 ggccgaggtg gccaatgtcg agcgcaagat gccgcgcgac tacatcagcg ccgatggctt
    38941 cggcatcacc gacgcctgcc gcgactacct gctgccgctg atccagggcg aggactaccc
    39001 cccctaccac aacggccttc cgcagtatgc caacctgaaa aaggtgctgg tgcccaagaa
    39061 gctgccgccg ttcaaccccg gaaagtgagc atcaggcgct gattgcatcc gctgcgaaga
    39121 ggcgtcagaa accgcccagc gcgcgcaggt gggccgccac cgagcgggcc agcgccggca
    39181 ggtggtaacc cccttcgagc accgagacga tgcgccccgc tgcatggcgc tcggccagcg
    39241 tcaccagctc ctgggtgatc cactggtagt cgctctcgtc gagccgcagg tgcgccatca
    39301 catcctcgcg gtggccatcg aaaccggccg agaccatgat caactgcggc ttgaagtggt
    39361 cgatgtaggg cagccagcgc cgccgcagcg cctcgcggaa ctccgcgccg ccggcaccgg
    39421 ccgcaatggg cgcgtggatg atgttctcgg ccggaaagtt gatgtcggtg aagggataga
    39481 acggatactg gaagctggag aggaacagca cccgctcatc gtgctgaaag atgttctcgg
    39541 tgccattgcc atggtgaacg tcgaaatcga ccacggccac ccgctccagt ccatgggcgt
    39601 gcagcgcatg cagcgttccg atggcgatgt tgttgaacag acagaatccc atcgcccggt
    39661 catgttcggc atggtgaccg ggcggacgca ccgagcagaa ggcccgctgc acctcgccgg
    39721 ccagcaccag atcgaccgcc cgcaccgccg caccggccgc gcgcagtgcc gctctcaggc
    39781 tgtcgggacc catcgcggtg tcctcatcga gatagaacag ccccgactgc ggaacggcct
    39841 cgcggatgcg ttgcaggtgg gcccgcccat gggccgcctc gatcgcctga tcctcggcca
    39901 gcggcgcatc gtaatgcttc agcagatccc acagttgcgt ctcgatcagg tgatcctgaa
    39961 tcgcgctcag acgctgcggg cactcgggat ggtggctgcc gctgtcatgc agcaggcagt
    40021 cgggatgggt gatgaaggca atcgacatca agcgactctc ggtgcacgga ttgggctgac
    40081 ttcggtcatg gtaacaaatc gccgttgcgc tatgctgcaa cggcgttgat caccgtcaag
    40141 agagaccgac atggaacatc gtcactacct cgcctcgctg ttcgagcccc gctcggtggc
    40201 cgtcatcggc gccaccgaac ggcccgacgc ggtcggccac ttcgtcatgc gcaacctgct
    40261 gggacacagc tatcagggca aggtctttgc catcaatccc aaccgcgaca gcattcacgg
    40321 cctgctggcc catcccgaca tcgaatcact gccgagcacg ccggaggtgg ccatcatcgc
    40381 caccccggcc cacaccgtcg ccgaactgct gcgcgcctgc ggccgcaagg gcgtgaagtt
    40441 cgccgtggtc tacagcggcg gctttgccga aatcggcgcg gccggcctcg atgcacagcg
    40501 tgaactcgac gccgccctgc gcgacagcgg cgtgcgcatc ctcggcccga acggcctcgg
    40561 caccgtgcgt cctgcactcg gttacgattg cagcatcggc catggtcagg tcgcccgcgg
    40621 caacctggcg ctggtgtcgc aatcgggcac gctgtgcaat gccatcatcg actgggccaa
    40681 ggtcaaccgc atcggcttct ccaacatcat cacgctcggt tcgagcctcg atgtcgacgt
    40741 cggcgaggtg ctcgactacc tgatcaacga cagccagacc gccggcatcc tgatctacct
    40801 cgaaggggtg agccatgcgc gccgcttcat cagcgccctg cgcgccgcct cgcgcgtcaa
    40861 gccggtcatc gccatcaagg tgggccgcca ccagagcggc tccagtgccg ccttcagcca
    40921 caccggagcg ctggtgggtc acgacgaggt gttcgacgcc atcctgcgcc gcaccggcgt
    40981 gacccgggtg cagaacattc ccgaactctt cgccgccgcc cgcaccctca cctcacgcta
    41041 ccgcgaatgt ggcgagcggg tggccatcgt caccaacggt ggtggaccag gcgctctggc
    41101 ggccgaccgg gcagccgacc tgcaactgac gctgccggcg ctcaaccacc gcaccctcga
    41161 acggctcgac cagtttctgc cacgcgactg gtcacgcgcc aacccggtcg acatcaacgg
    41221 cgacgcgacg cccgaacgct ttcgcgacgc ggtggctgcc tgcctggagg atgacgagat
    41281 cgacggcgtg ctggtgatcc tgacgccaca gggcatgacc gcgcccaccc aagtggcgca
    41341 ggcactggtc gaactggctg atcggcacgc caagccgatt ctctgctgct ggatgggcgg
    41401 cgagacggtg cgcgaagggc ggctgcggct gatggaggcc cgcattccgg tctttcagta
    41461 tccggagacg ctggtcggcg gctaccactg cctgctcacc caccgcaaga accagcaact
    41521 gctgctgcag atgccggcgc cgacacccga ccgcgcgccg cccgacctcg aacgggcccg
    41581 cgcgctgatt gccgaggtgc gcgccgacgg ccgcagcgca ctcaaccagc aggaggcgat
    41641 ggcgctgctg gcggcctttc acatcccggt accgcaggcc ggcatcgtgc agcgggtcga
    41701 tgcggcgatc gaactggccg agaagatcgg ctatccggtg gcgatgaaga tccactcccc
    41761 cgacatcgac cacaaatccg aagtcggcgg catcgccctc aacctcagaa ccgccgccga
    41821 actggtcgcc agttactggc agatgatgcg cgacattccc aagcggctgc ccgatgcgcg
    41881 gctcgatggc gtgctgatca ccgccatggc cacccggccg cgggcgcggg agctgctggt
    41941 cggcgtcacc accgacccga tctttggccc ggtcatcagc ctcggcaccg gcggcatctg
    42001 ggtcgaagcc tacgaccacc acgccgttgc gctgccgccg ctcaacgaag agctggccct
    42061 cgacctgatc cgcaatgtcc gcttcagcca cctgctcggc gcccaccgca gatttccggc
    42121 gatcagccag agcgcgctgg ccacggtgct gatccgggtc tccgaactgg tcgaggagtt
    42181 ccccgaaatc gccgaactcg acatcaaccc gctgctggtc gacgaacagg gcaccatcgc
    42241 cgtcgatgcg cgggtctacc tgcgcgaacc tgcgatccag tgcgacccct acgaccatct
    42301 ggccatctgc ccctatccca aacatctgat ccgcacctgg caactgcgcg acggcaccca
    42361 ggtcaccgtg cggccgatac gcccgaagga tgccgagatg gagcagaact tcgtccgcaa
    42421 cctctccgac cgcagccgct acctgcgctt ctggagcggg ctttcggagc tgaaccggcg
    42481 catgctcgcc gagttcacca aaatcgacta cggcacccga atgagcctga tcgtcacgct
    42541 ggagcaggat gggcaggaga tcgagattgc cgtcgcccag ttcagcacca ccgccaccgg
    42601 ccagagctgc gaattcgcca tcgtcgtcgc cgacggctgg cagggacagg gccttggcgg
    42661 caagctgatg gcctacctga tcgagattgc ccgctgccgc ggctaccgca gcatcgaagg
    42721 cgaagtgctc agcgagaacc gcgacatgct caacctgatg cgcgcgctcg gcttcaaggt
    42781 cgaaatcgac cccgaagatc tcagcgtcag acgggtggtg aagaacctgc tcgaaccgga
    42841 cccgccccgg cgcgggccag catcggcatg aaaagaggac ggatggtgcc ggagatagga
    42901 gtcgaaccta cgaccttcgc attacgaatg cgctgctcta ccaactgagc tacaccggcg
    42961 gggagcggta ttctagcaac ccgcgaccaa tagacaatgc cttggtgtgg gtggcgagcc
    43021 aatccggccc gccccaccgc aaaacggcca cgctgtgcca gaattgactg catgaaaaca
    43081 cccaaggtct ggttccccgc cattcgctgt ggcagtggtg cggatgtgtt caccgaacgc
    43141 ctctgccgcg cgctcaacgc ccaaggcatc cgcggcgaaa tcacctggct gccgcatcgg
    43201 gccgagtatc taccttggtc agt
//
LOCUS       DAORCQ010000005        42981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_432_length_42981_cov_6.266855, whole genome shotgun sequence.
ACCESSION   DAORCQ010000005 DAORCQ010000000
VERSION     DAORCQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 42981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42981
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_432_length_42981_cov_6.266855"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PKI23_00825"
     CDS             complement(<1..1178)
                     /locus_tag="PKI23_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNN35511.1"
                     /translation="MLITRGREQGFLTYAEVNDHLPEDISDPDQVEEIIQMINDMGIG
                     VFEETPDVDTLMMTEGDATADETATAEAVAVLAAVESEVGRTTDPVRMYMREMGSVDL
                     LTREGEIVIAKRIEEGVREMLCAMACYPGTVGKVLDEYARVGMGERRLAELISGYLDP
                     VVALPVEEVVPVVEEEAELAADVVTEESEESEVDTGPDPEEAARRFADLMLRFEASEQ
                     ALKTHGRGSKPADEALQAVGDVFKFFKFTPRIFDPMVASIRAVLTRIREQEREIMNLC
                     LREGQMARRDFITQFPGHEADPEWIDRVAAGHGKSKAKFRGLVAVRDEVQRLQRRLQA
                     IESEVGLTIGEIKEINRRMSLGEARARRAKKEMVEANLRLVISIAKKYTNRGLQFLDL
                     "
     gene            complement(1481..3289)
                     /gene="dnaG"
                     /locus_tag="PKI23_00830"
     CDS             complement(1481..3289)
                     /gene="dnaG"
                     /locus_tag="PKI23_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNN35512.1"
                     /translation="MAGLIPQSFIDDLLERVDIVDLVGERIRLRKSGANHFGLCPFHQ
                     EKSPSFSVNGDKQFYHCFGCGASGNALGFLIAYDRLEFPQAVEQLARRVGVALPEGEK
                     PSAQERQREAHHQLLAQVADHYRRQLSSGPAAETARRYLHQRGLDRDIIERFAIGFAP
                     PTAGSALLQQFPTQEQALLELGLIQKSDGGVARDRFRNRVIFPIRDPRGRTVGFGGRL
                     LGEGQPKYLNSSESPLFQKSHLLYGLHEAIQAQRQLDRLLLVEGYMDVVALAQHGLTH
                     AVATLGTATSVEHLKLAYRRVHELLFCFDGDAAGRRAAWRALEHLLPLLQEGQQARFL
                     FLPDGEDPDSLVRKEGAEAFAARSQQAQPLSEFFYSELCAQVDMGSIDGRARLVSLAR
                     PFLERLRTGSFRQLMVQRLAELTGIAAADLLLTSATQVEQPDPDSRPFDSMPHARPPL
                     HQRRTGRGRQPLPPAQAALLLLLKWPLLATRQACVNALRDFPTQGDSQLLREVLQQLQ
                     RSPGLGVAALLGRWHGTDQADLLARLAARESFLSQPQAEVELDALLAKLSGHGERRLL
                     DALLARPFDALTPDEKAQLKVLLESERAGASAPPRR"
     gene            complement(3355..3810)
                     /locus_tag="PKI23_00835"
     CDS             complement(3355..3810)
                     /locus_tag="PKI23_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003137358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNN35513.1"
                     /translation="MSSIPLKTQIAESVKQAMKAGEKRRVAALRLVMAEIKRVEVDER
                     IELDDARVITLLDRMLKQRRDSLDHFERAARDDLAEQERFEIATIQAFLPTPLTQQEI
                     AALIDEALGQLTVSGPQAMGAVMAWLKPRVQGRAEMASVSAAVKARLSG"
     gene            complement(3831..4046)
                     /gene="rpsU"
                     /locus_tag="PKI23_00840"
     CDS             complement(3831..4046)
                     /gene="rpsU"
                     /locus_tag="PKI23_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNN35514.1"
                     /translation="MPSVKIKENEPFDVALRRFKRACEKAGVMAEVRRREFYEKPTSV
                     RKRKAAAAVKRHAKKLQRESRRLQRLY"
     gene            4293..5342
                     /gene="tsaD"
                     /locus_tag="PKI23_00845"
     CDS             4293..5342
                     /gene="tsaD"
                     /locus_tag="PKI23_00845"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNN35515.1"
                     /translation="MLVLGIETSCDETGIGLYDSTRGLLGEALYSQTALHAEYGGVVP
                     ELASRDHIRKTLPLIRQVLAESGQRLRDLDGIAYTSGPGLMGALLVGAAIGRSLALAL
                     DRPAIAVHHMEGHLLAPMLEPDPPDFPFVALLVSGGHTQLVAVTGIGRYRLLGESLDD
                     AAGEAFDKSALLLGLDYPGGAALARLAEQGRPGRFRFPRPMLDRPGLDFSFSGLKTAV
                     LQVVETQRRAGTLDQQQRADIARSFEEALVETLREKCERALAETGYHRLVIAGGVSAN
                     LRLRAELTAMAERRSARLHAPRLRFCTDNGAMIALVGALRLAAGECTPATIETQPRWP
                     LEQLPPIPPLIHGVG"
     gene            5342..5698
                     /gene="folB"
                     /locus_tag="PKI23_00850"
     CDS             5342..5698
                     /gene="folB"
                     /locus_tag="PKI23_00850"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNN35516.1"
                     /translation="MDIVYIEDLRIETVIGIYAWEQEVKQVISIDLEMATDIRRAAAT
                     DRIEETLDYKAVAKRIIAYVQQNRPELVETLAERIAELIQREFAVPWLRLRLSKPGAV
                     RGSRAVGIIIERGERG"
     gene            5698..6219
                     /gene="folK"
                     /locus_tag="PKI23_00855"
     CDS             5698..6219
                     /gene="folK"
                     /locus_tag="PKI23_00855"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNN35517.1"
                     /translation="MAKVYLSIGSNIDREQHIRGCLDALAQRFGPLEISSVFESAAVG
                     FSGAPFYNLVVGIETELDPAALNQRLRAIEEAEGRRRDGAKFSGRTLDIDILLHGDRQ
                     GEFAGLRLPRPEITENAYVLWPLAELCPTRILPGSDQSIAMLWAGYDRDSQPLHPVDF
                     SWRGRAISPRSAS"
     gene            complement(6214..6960)
                     /locus_tag="PKI23_00860"
     CDS             complement(6214..6960)
                     /locus_tag="PKI23_00860"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNN35518.1"
                     /translation="MNEPIPVPKVALVTGAARRVGAAIVERLHGAGYQILLNYRHSAE
                     EAQALAARLNALRAGSVELLPADLCQMEAVQQLAELASQRGVGLLVNNASSFDPTPLG
                     AVSEAQWQALFDSNLKAPFFLLQALLPTLRARRGAVVNLIDIYAERPLQGYSVYSISK
                     AGLAMLTRSMARELAPDVRVNGVAPGAILWPEQPSAEQAAAQQRILAQTPLGRTGRLE
                     EIAEAVHFLAEATYVTGQIIAVDGGRSLYL"
     gene            complement(6957..8156)
                     /locus_tag="PKI23_00865"
     CDS             complement(6957..8156)
                     /locus_tag="PKI23_00865"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009404247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNN35519.1"
                     /translation="MKIYLVGGAVRDRLLGRPVTERDWVVVGATPAELLAQGFRPVGK
                     DFPVFLHPRSKEEYALARTERKAGRGYTGFVTCFDPSVTLEADLRRRDLTINAMAEDE
                     NGRIIDPYGGQRDLAGRWLRHVSAAFAEDPLRVLRVARFAARYAPLGFRVAPETLELM
                     RQISASGELADLVPERVWQELERALGEARPRLFIDLLRACGALGWLLPELDQRLECDG
                     GEPLLEALERVAMLSPQPEVRLALLLHDLDPEQPPGTAAHRAANLRLIDQICDRCRIP
                     NRHRQLARLLAAQSLTAHAALRLDAAALLQLLDSLDAWRRPERFEQFLLAAEADAGRW
                     PALAAESGYPQAAWLRRLHAACRQIDAGQLSGTLSGKAIGERLRQARLATIEQLRETL
                     TDSREHP"
     gene            8260..9513
                     /locus_tag="PKI23_00870"
     CDS             8260..9513
                     /locus_tag="PKI23_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN35520.1"
                     /translation="MYYGWRLLIALCAIYFLSIGTVFYGFAATMPDLIQAFGWSRSQV
                     SAGFSIASATLGLCGPLIAWMIRRFGTRMTIFMGGFSILIGALLTYTTQTVPQFYLSC
                     LFLGLGLAMQTIIPGTQLISNWFARRRALAIGLFMAAGGVGSGVLTPLFALLVKQTGD
                     WRIVWLIMTGAGMGSSLLALWVVKEMPADVGQQVDGVSETSSGSAKPVKQVRVYQTSH
                     PWETKAAFSTVVLWLAILCASMASLGGGLVNTQALLHLQDLGHEKVLAASSIGLVGFL
                     SVAGRVFSGTLGDYIEPKYMLSAGLLISLVGIYLLNIAHEPLLVYLFAILFGLGNGLA
                     VVTSPMIIANYFGSKNYASLFAVRSMIVTIIAALGPVLAGAWFDKFNSYSQVFWFYIG
                     MCLIAGIAALMMRPPIPAAQAVATS"
     gene            9534..10184
                     /locus_tag="PKI23_00875"
     CDS             9534..10184
                     /locus_tag="PKI23_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN35521.1"
                     /translation="MDKATEQPLRLPAPCGVLEVRYAAPTAGRPVAVICHPHPLFGGS
                     LDNKVVFTLMRSFQQLGLGTLRFNFRGVGASSGHYDDGRGELDDLAALCRWLAQQGNA
                     RLWLAGFSFGAYIAARGCQAAADWGVTVEQLILVAPPVGRFDFSPLQRFDCPVTLLQG
                     EADELVTADGVAAWAEQLHSPHQLLRFADCSHFFHGQLVELRSRLVAALAPTLVTA"
     gene            10230..11339
                     /gene="zapE"
                     /locus_tag="PKI23_00880"
     CDS             10230..11339
                     /gene="zapE"
                     /locus_tag="PKI23_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNN35522.1"
                     /translation="MSPLERYRASLAQPGFEHDPAQEQAVRHLQRLYDELVVTQPQST
                     GLLGRLSRPFRKPPTPIKGLYFWGGVGRGKTYLMDLLFDAVPIAEKARMHFHRFMREV
                     HAQLQARQGESDPLRGVAQAWSSRIRLLCFDEFFVSDITDAMLLGTLLEHLFNQGVIL
                     VTTSNIHPDGLYPNGLQRSRFLPAIALLKAHTEVVNVDGGIDHRLRTLTRAPLYYSPL
                     GQAADDNLMACFQRLGSTTIEADAVMTIDNRPIQTRYLVDDLAWFDFDQLCDGPRSQR
                     DYIELAELFETVVISNIPLLTTHHEDQSRRFINLVDEFYDCGVKLILSAAAPLAELYQ
                     GERLRFEFERTRSRLLEMQSHDYLARAHRRTTGGP"
     gene            complement(11370..11855)
                     /locus_tag="PKI23_00885"
     CDS             complement(11370..11855)
                     /locus_tag="PKI23_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNN35523.1"
                     /translation="MRPKPGWKQLTASPLDAWALGFGAGLSRWAPGTVGTALALPAWG
                     WLSSAAPLGLYLLLLGAAIVLGVWAAATATRRLGVDDHPAIVCDEMAAIGVVLLGVPA
                     EPLWLLLGFALFRLFDITKPWPVRWADRRIKGGWGIMFDDLLAAGYAWLALQAVRWLI
                     F"
     gene            complement(11855..12853)
                     /gene="thiL"
                     /locus_tag="PKI23_00890"
     CDS             complement(11855..12853)
                     /gene="thiL"
                     /locus_tag="PKI23_00890"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNN35524.1"
                     /translation="MALHEFQLIERYFSQPPLAAVGVAPCAGVRWGIGDDCALLDPPR
                     DASLAISIDTLVEGVHFLADADPFSIGWRALAVNLSDLAAVAASPSWFTLALVLPVVD
                     EFWLDGFSRGLTACAVRYGSLLVGGNLAQGPRSITLQVAGEVPAALALSRRGARVGDG
                     LYVTGTLGDAAAGLALLRGQLQADATDREFLSQRYLQPTPRIEAGLQLRGLASAMIDL
                     SDGLAGDLHHLISGSGVGFTLELNDLPLSAALCRVADRATARRWAFGHGDDYELCFTA
                     PRTHEARIEAALATVGVGCRRIGLATAAAGLTLTLDGQQQPADVALHGYRHFEAPQ"
     gene            complement(12875..13342)
                     /gene="nusB"
                     /locus_tag="PKI23_00895"
     CDS             complement(12875..13342)
                     /gene="nusB"
                     /locus_tag="PKI23_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNN35525.1"
                     /translation="MSDTPPPAAVRPKSKARHQARRLLLQALYQWQLAGASAAEIDRQ
                     FREEQPVRQADLDYFSELLRRIIALHGELDQVLAPLLDRPLEQLTPVERAILLIGSYE
                     LMHCIELPYPVVINEAIELAKSFAAPDSHKYVNGVLDRIAAQFRRPEVEARRR"
     gene            complement(13348..13830)
                     /gene="ribE"
                     /locus_tag="PKI23_00900"
     CDS             complement(13348..13830)
                     /gene="ribE"
                     /locus_tag="PKI23_00900"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNN35526.1"
                     /translation="MSQFQQISGDFTARDARFALVASRFNAFIVESLIAGAADVLLRH
                     GIPAGQITLIRAPGAFELPLVVRQAAESGRFDAIVALGAVIRGGTPHFDYVAGECVKG
                     LGQVSLSSNLPVAFGVLTVDTLEQAIERAGTKAGNKGAEAALCAFEMVSLLRQLQPAR
                     "
     gene            complement(13840..14967)
                     /gene="ribBA"
                     /locus_tag="PKI23_00905"
     CDS             complement(13840..14967)
                     /gene="ribBA"
                     /locus_tag="PKI23_00905"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNN35527.1"
                     /translation="MELNSIEELIEEIRQGRMVILMDDEDRENEGDLIMAASKVRPED
                     VNFMVRHACGLVCLTLTEARAQRLRLGPMVSDNRTAHATNFTASIEAAEGVTTGISAA
                     DRARTIQVAVAADAVAEDLVQPGHIFPLVAREGGVLSRAGHTEAGCDLARLAGLEPAA
                     VIVEIMNEDGSMARRPQLEQFAATHNLKIGTIASLIHYRILHEKTIIRLGSREVMTRH
                     GPFTLVSYRDTLQGDIHLALVCGEIDPDQPVLVRVQQADRLRDLLQIASSTASWSVHD
                     ALAAIAAAGQGVLVLIAGNEPPDATLASLARLEAVPQRRRVEQNPNVFYTVGTGSQIL
                     RDLGVRQMRLMSSPIKYNAISGFDLTVVEYLAADGRSIHLE"
     gene            complement(14984..15658)
                     /locus_tag="PKI23_00910"
     CDS             complement(14984..15658)
                     /locus_tag="PKI23_00910"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNN35528.1"
                     /translation="MFTGIIQAIGTVAALRPVGGDYRLTVEAGALDLRTAALGDSIAV
                     NGVCLTAVELSADRFSADLSIETLNRTTLQGLRAGSPVNLEKALTLATPLGGHLVSGH
                     VDGVGELLSLAPAGRSRLLRLRAPRELARYIAAKGSITVEGVSLTVNRVEGAEFELNI
                     VPHTLTATTLGGWQPGQPVNLEVDLLARYLERLLLGEQAAEGSAAPLTSRLLEQAGLV
                     TPTVSG"
     gene            complement(15660..16808)
                     /gene="ribD"
                     /locus_tag="PKI23_00915"
     CDS             complement(15660..16808)
                     /gene="ribD"
                     /locus_tag="PKI23_00915"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNN35529.1"
                     /translation="MTESEQDRRFMAEALQLAQRGLYGTDPNPRVGCLLVRQGAVVGQ
                     GWHHRAGEPHAEVEALRMAGAAARGATAYVTLEPCNHHGRTPPCSEALIAAGVARVVA
                     AMEDPNPRVAGGGLAALRRAGIAVECGLLAEAAAALNSGFIRRMGGGRPLLRLKLAMS
                     LDGRTALHNGVSQWITGPAARADVQRLRARSSAILTGSGTVLADDPALTVRDLDALGE
                     WAERPLRQPLRVVVDSQLRTPPGARLLHEAGETLIFTRQPLDEARAAQLRDRAHVRIE
                     AIAATAGQGVDLDAVLQRLAALQCNELLAECGPRLAGNLVQQGLVDELWIYLAPLLLG
                     SAARPLLQLPAIERMADRWPLLIDEIRPLGDDWRIRARFDHAAHATQE"
     gene            complement(16857..17324)
                     /gene="nrdR"
                     /locus_tag="PKI23_00920"
     CDS             complement(16857..17324)
                     /gene="nrdR"
                     /locus_tag="PKI23_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005887199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNN35530.1"
                     /translation="MHCPFCGTEETRVIDSRLVAGGDQVRRRRECTACQERFTTFELA
                     ELVMPKVIKRNGQREPFNEEKLRSGILRAIEKRPVSVEDVEAALNRIKHQLRATGERE
                     VASDLVGEAVMAQLQQLDEVAYVRFASVYRRFQHLDEFRREIERLEQKGGGEG"
     gene            complement(17345..18598)
                     /gene="glyA"
                     /locus_tag="PKI23_00925"
     CDS             complement(17345..18598)
                     /gene="glyA"
                     /locus_tag="PKI23_00925"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNN35531.1"
                     /translation="MFDRNLTLADFDSDLWAAMQGESRRQEAHIELIASENYTSPLVL
                     AAQGSVLTNKYAEGYPGKRYYGGCEYVDQVEQLAIDRAKALFGADYVNVQPHSGSQAN
                     AAVYMALLDPGDTVLGMDLAAGGHLTHGAKVNFSGKIYHSVSYGLDPASGRIDYDQVE
                     TLARTHRPKMIMTGFSAYSRLVDWQRFRAIADSVGAFLVADMAHVAGLVAVGAYPSPV
                     GIADVTTSTTHKTLAGPRSGLILAKANPEIEKKLNSAVFPGTQGGPLMHVIAAKAVCF
                     KEAMSPEFKLYQQQTLANARAMAAQFIARGYRIVSGGTDNHLFLLDLTDKRITGKEAE
                     EALGRAHITVNKNAVPNDPRSPFVTSGIRIGTPAVTRRGFDSGEVEQLTHWICDLLDD
                     LGNDATCQRIRQQVSEICARHPVYG"
     gene            18719..19141
                     /locus_tag="PKI23_00930"
     CDS             18719..19141
                     /locus_tag="PKI23_00930"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNN35532.1"
                     /translation="MDQPPTRLAGEIFHHTDRRRVSRIRCYAPVLVESTAGCYPAALA
                     DLSLQGALLQVPQWQPAVNECATLLVELDTQGIQLILEARVRHQHDARIGVQFTEVND
                     LQQRCLRQFLFRGTADQKQLPRTWRNLFGRGPIRQPPQ"
     gene            19289..20728
                     /locus_tag="PKI23_00935"
     CDS             19289..20728
                     /locus_tag="PKI23_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNN35533.1"
                     /translation="MRNYDKLYINGEWVAPHGKGSSQVTNPFNNEVIGSVPQGDLQDV
                     DRAVQAAKAAFPAWAATSVEERAQYLEKLVGAMSKRAKELSSVISQELGMPISQAEMI
                     QVGMAIGTLNSFPALLRSYKFEKQINNSTVVREPIGVCGFITPWNYPLYLIVAKAAPA
                     LAAGCTMVLKPSRETPLNAFIFAELINEVGLPPGVFNLVSGPGGVVGEGLAAHPDVDM
                     VSITGSTEAGISVAKSAANTVKRVAQELGGKSANIILDDADLNTAVAAGVRGVMMNCG
                     QTCAALTRMLVPESRKDEVIAIAKATAESITQDDPSKEGFHMGPLSSLQQANTVRKYI
                     RKGIEEGATLVTGGLEEPAGFEGGAFVKPTIFADVKNSMTIAQEEIFGPVLSILTYKD
                     EADAIRIANDTVYGLSGSVWSSDPNRALRVAKQLRTGQVSINGGGMNLMAPFGGYKQS
                     GNGRELGEFGLEEYLEIKSVQMPPAAAAS"
     gene            complement(20922..22217)
                     /locus_tag="PKI23_00940"
     CDS             complement(20922..22217)
                     /locus_tag="PKI23_00940"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020312432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNN35534.1"
                     /translation="MGKNVIILGTQWGDEGKGKIVDLLTDQAAAVVRFQGGHNAGHTL
                     VIDGAQTVLHLIPSGILREGVTCVIGNGVVVSPVALFEEIAMLEARKVPVRERLRISA
                     ACPLILPSHVALDQAREQARGAAKIGTTGRGIGPAYEDKAARRGLRLGDLFEPERFAS
                     KLRDVLDYHNFILERYHQQAPIDFQQTLEHNLALAEQLRPMVTDVTGLLHRLRRDGAN
                     LLFEGAQGALLDIDHGTYPFVTSSNTTAGGAATGSGMGPLQFDHVLGITKAYTTRVGS
                     GPFPTELFDATGAHLAKRGHEFGSTTGRARRCGWFDAVILRRSVELNSLSGLCITKLD
                     VLDGLDLIRVCIGYQDASGQPLPIPLDSEGYDQVVPIYEELPGWSESTLGLTRLQELP
                     ANARAYLDRIEALVGAPIDIISTGPDRNETIVLRHAFGG"
     gene            complement(22271..23452)
                     /locus_tag="PKI23_00945"
     CDS             complement(22271..23452)
                     /locus_tag="PKI23_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNN35535.1"
                     /translation="MTIADRWLLPDGIEEILPPDAQVHEQLRRQLLDLYRSWGYELVM
                     PPFIEYLESLLIGAGHDLDLKTFKVTDQLTGRMMGIRADITPQVARIDAHALRRNGPV
                     RLCYAGSVLHTRTDQPFDSRGPIQVGAELYGHAGIDSDLEVIALMLEGLMKIGLTDLS
                     LDLGHVAIYRTLAESSQLAPAIQQELFELMQAKACTEIDQLLDRQPVPAPLLRELPHL
                     HGEPGLVLPRSRQLLAATPAPVQQALDDLEQVVRRIGRLYPSVALYCDLSELRGYHYH
                     TGLVFAAYTSDHGREVAKGGRYDHIGEAFGRARPATGFSTDLKSLFELRRPADPVPGA
                     IFVPQPSDEPAHRAVIDQLRAAGERVISALPGDSGSVGDLGCDRQLLFSAGGQWRLVE
                     L"
     gene            complement(23505..23705)
                     /locus_tag="PKI23_00950"
     CDS             complement(23505..23705)
                     /locus_tag="PKI23_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNN35536.1"
                     /translation="MTGVGWHELLVALCLLLVLEGILPFVAPEQWRGLLQQIARLDRR
                     AIRLLGLGSMLTGAGLLHLINH"
     gene            complement(23750..24634)
                     /gene="hflC"
                     /locus_tag="PKI23_00955"
     CDS             complement(23750..24634)
                     /gene="hflC"
                     /locus_tag="PKI23_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNN35537.1"
                     /translation="MGNRSMLALLLGALLTLAGYQALYTVSETERAVLLEFGRVVQAD
                     IQPGLHLKKPFINEVKKFDARTTAIDAQPQRFFTLEKKALIIDSYVMYRIADVQTYYT
                     ATSGDESQAARLLAQRVNNGLRDQVGERAMHDVVSSEREQLMKDLIVKLGNIIRGELG
                     IELVDIRIKRIDLPEDVSSSVYDRMKSERERLAREYRSRGKEQAEMLRAQADREKAVI
                     SANAYRDAEKLRGEGDAVASATYAAVYKVDPEFYGFYRSINAYSKSFSDRKDLLVLSP
                     TSDFFKYFKEREGAPRAR"
     gene            complement(24635..25819)
                     /gene="hflK"
                     /locus_tag="PKI23_00960"
     CDS             complement(24635..25819)
                     /gene="hflK"
                     /locus_tag="PKI23_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNN35538.1"
                     /translation="MAWNEPGGGEPKDPWGNNRGNQHQGPPDLDELLRKLGATLGGLL
                     GGGGKGGGTAQGDGGSGMGGLLLGLLVLLLFLLGYNALYTINQQERGIVLRFGRYESS
                     VMPGLHWKIPLIDALYKVNVTRVRDRNHRSLMLTEDENIVDVSVTVQYVVSDPKDFLL
                     RVRDPESALENATESALRHVVGTSKMDRVISEGREQIGIDVHSKMQEYLNLYQSGIQI
                     SKVNIEEAKPPKEVQAAFDDVIKAREDEARFRNEAEAYANGIVPEARGYAQRQIQEAE
                     GYKEGVIARAQGDALRFQSMVAEYQKAPELTRQRLYIDAIESVLGNSSKVLIDQSGGN
                     NMIYLPLDRLAGAATTSTLPPVSTTPAFTADSGVDVSRLTDRVIEELRNRQNSSRSRE
                     SR"
     gene            complement(25871..27157)
                     /gene="hflX"
                     /locus_tag="PKI23_00965"
     CDS             complement(25871..27157)
                     /gene="hflX"
                     /locus_tag="PKI23_00965"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNN35539.1"
                     /translation="MLVQLDLPGDSADLDELHELARSAGLEPVLRFRASRAKPAPGHF
                     LGSGKVDELAAAALQFDAGIVLFNHELSSSQERNLERRLGRRVLDRTGLILIIFALRA
                     RTHEGQLQVELAQLQHLSSRLVRGWSHLDRQKGGVGLRGAGETQLELDQRMLRQRTQR
                     VEQRLEAVRKRRAEGRRARRRGEIPVLSLVGYTNSGKSTLFNALTGADTFAADQLFAT
                     LDSTLRRIELPHLGPAILADTVGFIRQLPHQLIEAFRATLEESREADLLLQVIDSADA
                     DQQERSQEVEAVLAEIGADTVPRLRICNKIDLVDGLSPRLERDSQGRPTRVWLSARSG
                     AGLDLLRQALAELLQPDWVEEELRLPPSLGRLRARLYALGAVFGETTDESGISRVAVR
                     FSGQALSRLLAREGIDPAALGGSQRLRQLAASPAPP"
     gene            27104..27235
                     /locus_tag="PKI23_00970"
     CDS             27104..27235
                     /locus_tag="PKI23_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35540.1"
                     /translation="MQFVQVGAVAGQVELNQDGALADLGTVEELKRSDRGDYSLPPS"
     gene            complement(27214..27483)
                     /gene="hfq"
                     /locus_tag="PKI23_00975"
     CDS             complement(27214..27483)
                     /gene="hfq"
                     /locus_tag="PKI23_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNN35541.1"
                     /translation="MSKGHSLQDPYLNVLRKERIPVSIYLVNGIKLQGQIESFDQFVV
                     LLKNTVSQMVYKHAISTVVPARPVRLPPAGNADMEDEEPYEGGNE"
     gene            complement(27561..28514)
                     /gene="miaA"
                     /locus_tag="PKI23_00980"
     CDS             complement(27561..28514)
                     /gene="miaA"
                     /locus_tag="PKI23_00980"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNN35542.1"
                     /translation="MTLPDDTPLALLLLGPTASGKSALALELADRFAVEIVSVDSAQV
                     YRGLDIGSAKPTPALQARYPHHLIDLRDPADPYSAAAFRDDALAVMAQIRARGNTPLL
                     VGGTMLYFQLLCRGITTLPPARPALRARLLDEAEADGWSRLHQRLWQVDPVAAEQIPL
                     GNRQRLIRALEVFIDTGIPLSQHHAEQQAAPLPQRWVTLGIQPQSRQLLSGPIAQRFR
                     QMLDMGFVEEVQRLFERGDLHPDLPAIRAVGYRQVWSHLAGQSSHAEMIERSLIATGQ
                     LAKRQLTWMRGWPGLRPLPLERAEQLALALKIVTPLPISRR"
     gene            complement(28529..30229)
                     /gene="ilvA"
                     /locus_tag="PKI23_00985"
     CDS             complement(28529..30229)
                     /gene="ilvA"
                     /locus_tag="PKI23_00985"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNN35543.1"
                     /translation="MGAGENLAHARHSDVEVGRHCAPRIGGCQSLRRPSTGLTAAAPC
                     TIACCHRPTYRQRAATMPESYIRRILEANVYDVAIETPIQPAPLLSRRYRNQILLKRE
                     DLQPVHSFKLRGAYNKMVKLTPEARAAGVICASAGNHAQGVALAAQRLKIRATIVMPR
                     TTPAIKVAAVRALGGKVLLQGDSFDEAFIRSQQLMAEQQLTYIHPYDDPDVIAGQGTI
                     GMEILRQHTGALDALFVPVGGGGLMAGIAAYIKYLRPEIRLIAVEAEESACLAAALAK
                     RRRVVLPQVGLFADGVAVAQIGQETFRVLRKSVDEVITVTTDEICAAIKDLFSDIRAI
                     AEPAGALALAGLKQYVERNQVEQQTLMALLSGANVNFDRLRHIAERAELGERREAVLA
                     VTIPERPGSFHAFCTLLGRRNITEFNYRYNDPQQARIFVGVEVNEGAPGEGRAALIEK
                     LHHHGYPVVDMTDNEMAKLHIRHMVGGHATDVADERLYRFEFPERPGALLNFLSRLGE
                     RWNITLFHYRNHGAAYGRVLSGIQVPRSERARLNQELAAIGYPWWDESENPAYRLFLG
                     "
     gene            30203..31216
                     /gene="hemB"
                     /locus_tag="PKI23_00990"
     CDS             30203..31216
                     /gene="hemB"
                     /locus_tag="PKI23_00990"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNN35544.1"
                     /translation="MSQILSRPHPRFRPRRMRRDDFSRRLMRETRLSSDDLIYPLFVV
                     EGNNRREPIGSMPGIERVSIDQLLHEAEAALQLGIPAIALFPVTPAQLKSSDGAEACN
                     PQGLAQRAIRAVKARFPELGVIADVALDPYTSHGQDGIIDASGHVLNDVTIEVLVRQA
                     LSQAEAGADIVAPSDMMDGRIGAIRQALEGAGHSHTRILAYSAKYASAYYGPFRDAVG
                     SAGNLGKSDKKSYQMDPANLEEALQEVALDLEEGADMVMVKPGMPYLDVVQRIKRQFG
                     VPTLVYQVSGEYAMHRAAIANGWLSEAVIMESLLSIKRAGADAILTYFAKEVATELRR
                     FGG"
     gene            31304..31630
                     /gene="trxA"
                     /locus_tag="PKI23_00995"
     CDS             31304..31630
                     /gene="trxA"
                     /locus_tag="PKI23_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNN35545.1"
                     /translation="MSDAIVHVSDETFEQEVLKSPTPVLVDYWAAWCGPCRMIAPVLD
                     ELADDYDGRVKVCKVDIDQNEMTPAKYGVRGIPTLMLFKNGQVEATRVGAMSKSELTT
                     FIDSNL"
     gene            31840..33189
                     /locus_tag="PKI23_01000"
     CDS             31840..33189
                     /locus_tag="PKI23_01000"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN35546.1"
                     /translation="MTPAHPHDDIGLLFSLLPLPLQHALSLLPREQLLEIVLDLGRVP
                     QARLRDGAVMLGSEPISHDQLQQIVGRISPFGQDNRAGIEGTLHRLSALRNRRGEIVG
                     LTLRVGRAVIGTIGPLRDLVERGASLLLLGRPGVGKTTRLREVARFLADELGRRVMVV
                     DTSNEIGGDGDIPHPAIGCARRIQVPTPEKQHAVMVEAVENHMPEVIIIDEIGTAADV
                     LAARTIAERGVQLIGTAHGNTLANLLANPTLCDLIGGVQTVTLGDEMARLRGTQKTIL
                     ERKGPATFDCVVELISQDELLIHADSNGSVDALLRGESPPVLHCGDAAAEPADGESGV
                     VQSLLPRRRRLTPSSQTPPLVSSGHPLMIYPYGVDRDLIERVLRDLRLHARTVRRVER
                     ANLILALREQVEDQRLLRMVQATGIPVYFLKRNSTAQIRHLLQHNLELQGVAANGTH"
     gene            complement(33202..37302)
                     /locus_tag="PKI23_01005"
     CDS             complement(33202..37302)
                     /locus_tag="PKI23_01005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNN35547.1"
                     /translation="MQQPDRLEETAEALPFHPAVARWFERTFGGPTPAQRAAWPAIQR
                     GEHTLIAAPTGSGKTLAAFLAVIDRLLRQGLTGELVEATAVVYVSPLKALSNDIQRNL
                     AWPLAGIASELEVLGLPAVPLRVGLRSGDSTPAERTALLKRPPHILVTTPESLYLLLT
                     SSGGRALFGQTHTLIIDEIHALIDSKRGAHLALSIERLQHLARQPLTRIGLSATQNPI
                     AAVADFLVGAGDGVAPPPCHIIDQGHRRALDLDLLLPAAPLETVMSNEVWGLLYDQLA
                     ALILEHRTTLIFVNTRRLAERVTRQLSERLGSEAVAAHHGSLSKAQRFEAEQRLKAGE
                     LRALVATASLELGIDIGAVDLVCQLGATRAIATLLQRVGRSGHSVGGLPKGRLLPLSR
                     DDLVEMTALIDAVKRGELDQLRIPQAPLDVLAQQIVAEVAAEPWSERALYALLSRAQP
                     YRALPYDTFAELVRMLGEGYSSRRGRRGACLYRDLLQGMLRPRPGARLTALTCGGTIP
                     DTADYDVILEPAGTLIGSVNEDFAVESMAGDIFQLGNSSWRIVKVESGRLRVEDAAGQ
                     PPTIPFWLGEAPARSDALSRAVSRLRREVDESLQQGSVETAARALQARLGLPAAACWQ
                     LVEYLAAARAALGAMPSDDCLVLERFFDESGGMQLVLHAPFGGRINRAWGLALRKRFC
                     RQFNFELQAAASENSIILSLGVTHSFPLAEVAGYLQARTVRPLLVQALLDAPLFQARW
                     RWNALTALAIPRRRGGQRTPAFLQRIQAEDLIALVFPDQLACFENLQGEREIPDHPLV
                     AQTLDDCLTEAMDIDGLERLLQRLVAGEIRLVCAELTEASPFAQEILAARPYAFLDDA
                     PLEERRTQAVISRRWLDPATAADLGRLDAAAIERVRGEAWPVAHDGDALCDALLLVGL
                     LHEEELAQPRTAGWSTLLTAAQAQGRVLCIELAGERRWLAIERLPQLLALHPQLAEQP
                     LPPLPDPLKEFDWTAEAALVELLRARLESSGPLDADELASLFALPPPRIMAALLKLEQ
                     EGFILRGAFSDPQREQWCERRLLARINRYTLDRLRRAIEPVSIAQFHRFIDHWQGVGA
                     SGSGVERLNAVIGQLAGLEATASQWEGELLPARLAHYDPAWLDQLTLSGRVVWTRLGG
                     SGKGGGPLRRTPIMLLPRGEFPLWRALADADQGTPCSAGAERVRALLASGGALFFDEL
                     CQRLSLLPSHLERYLAELVQRGEVSADSFAGMRGLIQPAAWKNHPRYRRRGSSALAEA
                     GRWSLLPPPLQQEQAASWFGARHSEQVTRAIAGQLLRRYGIIHRQLLERESRLLPPWR
                     DLLRIYRRLEASGEIRGGHFIAGVSGEQYALPEAIGALRAVRHEEG"
     gene            complement(37386..37763)
                     /locus_tag="PKI23_01010"
     CDS             complement(37386..37763)
                     /locus_tag="PKI23_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HNN35548.1"
                     /translation="MASPSQCGPDGRGVGFYFRNVTELILFGVRGKGTRTLAPGRRQV
                     NFLATMKREHSRKPDEAYDIIEACSPGPWLEMFARGSRTGWTTWGQQADDYFPTWETY
                     ANHSQVGRDETSARATADGATSL"
     gene            complement(37863..38165)
                     /locus_tag="PKI23_01015"
     CDS             complement(37863..38165)
                     /locus_tag="PKI23_01015"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNN35549.1"
                     /translation="MAEIVWTEEAGRWLRDIHDYIAVDNPVAASKVMAGIIERAQVLR
                     HFPEIGHRYRVEPEGEIRILLYGHYRIVYLRRPSPSQLIDILGVFHGALDLARYLP"
     gene            complement(38153..38338)
                     /locus_tag="PKI23_01020"
     CDS             complement(38153..38338)
                     /locus_tag="PKI23_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35550.1"
                     /translation="MSNVKEKMAEVIQSQPEDATYEEIMRELLFEGMVERGLEDARQG
                     RVMTQNELEQRIRTWQK"
     gene            38540..38689
                     /locus_tag="PKI23_01025"
     CDS             38540..38689
                     /locus_tag="PKI23_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNN35551.1"
                     /translation="MPRRLLLLCVTLCCLAGCGQRGPLYLPPPTGDQSAPAASTPPSP
                     LRNPS"
     gene            38689..39933
                     /gene="lysA"
                     /locus_tag="PKI23_01030"
     CDS             38689..39933
                     /gene="lysA"
                     /locus_tag="PKI23_01030"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNN35552.1"
                     /translation="MDPFGYRQGRLHAEDVPLEQIAAEFGTPCYVYSRAAIEQNYLAF
                     EQPLQGQPHLLCYAVKANSNLAVLNLLARLGAGFDIVSVGELERVLAAGGDPARVIFS
                     GVGKRADEMARALDVGVRCFNVESIPELDRLEAVARAHGQRAPISIRVNPDVDARTHA
                     YIATGRKENKFGIEFADALALYGRAAASPHLEVVGVDCHIGSQLTEIEPFLVALERML
                     ELIDQLRAQGIEIRHLDLGGGLGVRYRDETPPTPAQYITALTRRLAGTGLELVLEPGR
                     AIVANAGLLLSRVEYLKQSSVKRFAIIDAAMNDLLRPTLYNAWQAVCEVTRRGGEPVE
                     YEVVGPVCESGDFLAKARLLDIEAGDLLAIRSAGAYGFVMASNYNSRGRPAEVMVSGT
                     QMHLVRPRETLASLYADERLLP"
     gene            39982..40812
                     /gene="dapF"
                     /locus_tag="PKI23_01035"
     CDS             39982..40812
                     /gene="dapF"
                     /locus_tag="PKI23_01035"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNN35553.1"
                     /translation="MRLRFTKMQGAGNDFMVVDLVTQQAVLTPTQIRQLADRHFGVGF
                     DQLLLVEPPTDPGIDFKYRIFNADGGEVEQCGNGARCFARFVRDQRLSWKERITVETR
                     GGLLELLLERDGSVTVDMGAPDFSPKALPFQAEAEALHYPLEVAGQTLEIGAVSMGNP
                     HAVLVVDDVERAPVTTLGPAIGSHPRFPKGVNVGFMQIVDRTTIKLRVHERGAGETLA
                     CGTGACAAAVIGMRRGQLDEQVTVRLTGGELLIRWAGNGAHVWMSGPATRVYEGWIQL
                     "
     gene            40809..41525
                     /locus_tag="PKI23_01040"
     CDS             40809..41525
                     /locus_tag="PKI23_01040"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNN35554.1"
                     /translation="MTISGQAGGNSHGETSAPSEAEVAAYLHLHRDFFGRHEPLLAEM
                     YFPSPQAGAISLVERQLGILRERLQSTRHELALLMGRAEQNDRLITLTQTLSLQLLDA
                     AHGSEINLLLRRALIGELGNCALSLLVATPGTAAAALGFHTPASAEAERRVEAVLANR
                     CASLGLFRDEELAALFGSEGSGVGSAVLLPIQRGPVQAVLALGHPDRDHYRAGMGTLF
                     ISHLGEVIGRVIERLLKSAA"
     gene            41540..42442
                     /gene="xerC"
                     /locus_tag="PKI23_01045"
     CDS             41540..42442
                     /gene="xerC"
                     /locus_tag="PKI23_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNN35555.1"
                     /translation="MDAQIDHFLRQLQQQRALSNHTLAAYRHELTRFARFAGERQLQL
                     ADIDQPQIRLFASELRRHGLGGRSIQRALSALRSFFRFLLREGVVGDNPVLGVRLPGS
                     RTRLPRPLPVEEAVQLVELKGEAWLIRRDRALLELCYGAGLRLAELTSLNCADLDLNE
                     RLVTVTGKGRKQRLVPFGRAAATALRQWLTVRGARLADDREPALFISRRGQRIHPRTV
                     QAMMHRRGVEQGLTSRLHPHRLRHSFATHLLESSGDLRAVQELLGHRDISTTQIYTHL
                     DFQHLAASYDAAHPRARRRGKSVP"
     gene            42439..>42981
                     /locus_tag="PKI23_01050"
     CDS             42439..>42981
                     /locus_tag="PKI23_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNN35556.1"
                     /translation="MTPPRLKLVTFDLDDTLWESAPVLIEAEAAMQRALQQQLPALGD
                     TLRSGILRQQRALLLAAEPALIHRVSELRRRVIATTLRDLGLDAERARQITEAAFEQF
                     LQARHQVRYHADALEMLELLARRYRLGALTNGNADIRRLGLDRYFDFGFSAEMLGVGK
                     PDPALFRAALDAIRCSPAQMV"
BASE COUNT         7176 a        14730 c        13965 g         7110 t
ORIGIN      
        1 atcaggtcga ggaactgcag gccgcggttg gtgtacttct tggcgatcga gatcaccagc
       61 cgcaggttgg cctcgaccat ctccttcttg gcacggcgcg cgcgggcctc gcccagcgac
      121 atgcgccggt tgatctcctt gatctcgccg atggtcagac ccacctcgct ctcgatcgcc
      181 tgcaggcgcc gctgcagccg ctgcacctca tccctgaccg caaccaggcc gcggaatttg
      241 gccttgctct tgccatggcc ggcggcaacc cgatcgatcc actccgggtc ggcctcgtgg
      301 ccggggaact gggtgatgaa gtcgcgccgc gccatctgcc cttcgcgcag acagaggttc
      361 atgatctcgc gctcctgctc gcgaatccgg gtcagcaccg cccggatcga agccaccatc
      421 ggatcgaaga tacgcggggt gaacttgaag aacttgaaga catcacccac ggcctgcagg
      481 gcttcgtcgg caggcttgct gccacgacca tgggttttca gcgcctgctc gctggcttcg
      541 aacctgagca tcaggtcggc aaaacggcgt gcggcctctt ctggatcggg accggtatcg
      601 acctcgctct cctcgctctc ttcggtgacg acgtcggcgg ccaattcagc ctcttcctcg
      661 acgaccggca ccacctcctc gaccggcagc gcgacgaccg gatcgagata accgctgatc
      721 agctcggcca agcggcgctc acccatgccg acccgggcat attcatcgag caccttgcca
      781 acggtgccgg gatagcaggc catcgcacag agcatctcgc gcacaccctc ttcgatgcgc
      841 ttggcaatga cgatctcacc ttcacgggtc agcaggtcga cgctgcccat ttcgcgcatg
      901 tacatccgca ccggatcggt ggtgcgaccg acctcgctct ccacggcagc cagcactgcg
      961 acggcttcgg cggtcgcagt ctcatccgcg gtggcatcgc cttcggtcat catcagggtg
     1021 tcgacatcgg gggtctcctc aaagacacca atccccatgt cgttgatcat ctggatgatc
     1081 tcttcgacct gatcagggtc ggaaatgtcc tcaggcaggt ggtcgttcac ctccgcgtag
     1141 gtcaggaaac cctgctcccg tccccgggtg atgagcattt tgatacggga ctgctgctgt
     1201 tccatttcgg acattgcaca cctttagcaa agtggtcgga ccggatcggt ccgacgaaga
     1261 gcaaaaacca acgcttcaac tccgatcaag atagtcgcaa gcgctgttcg atgtggtgat
     1321 ctttcccgca aaagcaagga ggggatgcac aatcagacaa aaggttctgg attggatagc
     1381 tcgccggcca aaaaattccc gtgatcgacc aggattcatt cgcaggcatc tcgcccaacc
     1441 aaacgcgaaa tactaccaga aatttttcaa tgatgctgga ctaccgccga ggcggcgcag
     1501 aggctcccgc tctttcgctc tccagcagca ccttcaattg cgctttttcg tccggcgtca
     1561 acgcatcgaa cggacgggcc agcaacgcat cgagcagcct gcgctcgcca tggccactca
     1621 gcttggcgag cagcgcatca agctccacct cggcctgcgg ctgcgacaga aaactctccc
     1681 gtgccgccag tctggccagc agatcggcct ggtcggtgcc atgccagcgg ccaagcagtg
     1741 cggccacccc aagcccgggc gacctctgca actgctggag cacttcgcgc agcaactgac
     1801 tgtcgccctg cgtcggaaaa tcacgcagcg cattgacaca ggcctggcgt gtcgccagca
     1861 gcggccactt cagcagcagc agcagcgcgg cctgggccgg cggcagcggc tgacgcccgc
     1921 gcccagtgcg ccgctgatgc agggggggac gggcatgagg catcgagtca aacgggcgcg
     1981 aatcgggatc gggctgctcg acctgcgtcg ctgatgtcag cagcagatca gccgcggcga
     2041 tgccggtcag ttccgccagc cgctgcacca tcaattgccg gaatgaaccg gttcgcagcc
     2101 gctccagaaa cgggcgcgcc aggctgacca gccgcgcccg gccatcgatg ctgcccatgt
     2161 cgacctgcgc gcaaagctcg ctgtagaaga attccgacag cggttgcgcc tgctggctgc
     2221 gcgccgcaaa ggcctccgcc ccctccttgc gcaccagcga gtccggatcc tccccgtcag
     2281 gcagaaacag aaagcgtgcc tgctgcccct cctgcagcag ggggagcaga tgctccagtg
     2341 cccgccaggc ggcacggcgc cccgctgcgt caccatcgaa gcagaacagc agctcatgga
     2401 cgcggcgata ggccagcttg agatgttcga cactggtggc ggtgccaagc gtcgccactg
     2461 catgggtgag gccatgctgc gccagcgcca ccacatccat gtaaccctcg accagcagca
     2521 accggtccag ttgccgctgc gcctggatcg cctcatgcag gccgtagagc agatggctct
     2581 tctggaacag tggcgactcg gaggagttga ggtacttggg ctgcccttcg cccagcagcc
     2641 ggccaccgaa gccgaccgtg cggccgcgcg gatcgcggat cggaaagatc acccgattgc
     2701 gaaagcggtc acgcgcgaca ccgccgtcac tcttctggat cagccccagt tcgagcagcg
     2761 cctgctcttg cgtcggaaat tgctgcagca gggcgctgcc ggccgtcggc ggcgcaaagc
     2821 cgatcgcaaa gcgctcgatg atgtcgcggt cgagaccgcg ctgatgcaga tagcgccttg
     2881 ccgtctcggc agccgggccg ctggagagtt ggcgccggta gtggtcggcc acctgcgcca
     2941 gcaactggtg gtgcgcctcg cgctggcgct cctgcgcact cggcttctcg ccttcaggca
     3001 gcgcgacacc gacccgccgc gccaactgct cgaccgcctg cggaaattcg agtcggtcgt
     3061 aggcgatcag aaatcccaac gcgttgccgc tcgcgccaca gccgaagcag tgatagaact
     3121 gcttgtcgcc gttgaccgag aacgaggggc tcttctcctg atggaacgga cagaggccga
     3181 agtggttggc gcccgacttg cgcagccgga tgcgctcgcc gaccagatcg acgatgtcga
     3241 cccgctcgag caggtcatcg atgaaggatt ggggaatcag accggccatg acgcttcaca
     3301 acagtggccc cggactggag ccgaccgcca ctgtgcgcga aaccgaggcg tggatcaacc
     3361 gctgagtcgg gccttgacgg cggcactgac gctggccatt tcggcgcgtc cctgcactct
     3421 gggcttgagc caggccatga ccgcgcccat cgcctgcgga ccgctgaccg tcagttgccc
     3481 gagcgcctcg tcgatcagcg cggcaatctc ctgctgtgtc agcggtgtcg gcagaaaggc
     3541 ctggatcgtc gcgatctcga accgctcctg ctcggccaga tcgtcgcgcg cagcacgctc
     3601 gaagtggtcg agcgagtcgc gccgctgctt gagcatgcgg tcgagcagcg tgatgacccg
     3661 ggcatcgtcg agctcgatgc gctcatcgac ctcgacacgc ttgatctcgg ccatgaccag
     3721 ccgcagcgcg gcgacgcggc gtttctcgcc ggccttcatc gcctgcttga ccgattcggc
     3781 gatctgcgtc ttgaggggga tggagctcat gctgcgcggc agcgagggga tcagtagagt
     3841 cgctgcaggc ggcgcgattc gcgctgcaac ttcttggcgt ggcgcttgac ggccgcggca
     3901 gccttgcgtt tgcgcaccga agtgggcttt tcatagaatt cgcggcgacg cacctcggcc
     3961 atgacaccgg ccttttcaca ggcacgcttg aagcggcgca gcgcgacatc gaagggttcg
     4021 ttctctttga tcttcaccga cggcatatag gatcctgtgt gggtttgatt ccaacgccca
     4081 tgacaatggg ccccggcttg atggagcggg gccggcattc tagcaccgat cggaagcggt
     4141 acaaaccatc gggagaattt cgaccggtgg gcgacaggcc catcgaggcg aagcgtgatc
     4201 accacgcggc ccaacgattc cacacgccgg tcgggacggc tatcatcgtt cccccttctg
     4261 gagctctgtc tccgctcaat cactgaagca ccatgctggt acttggcatc gaaacctcct
     4321 gtgacgagac cggcatcggc ctctacgaca gcacccgcgg cttgctgggc gaggcgctct
     4381 acagtcagac ggcgctgcat gcggagtatg gcggcgtggt gccggagctg gcgtcgcgcg
     4441 accacatccg caagacgctg ccactgatcc ggcaggtgct ggccgagtct ggacagcgtc
     4501 tgcgcgacct cgatggcatc gcctacacca gcggacccgg tctgatgggg gcgctgctgg
     4561 tgggcgcggc catcggccgc tcgctggcac tggcgctcga ccggccggcc atcgcggtgc
     4621 accacatgga gggccatctg ctggcgccga tgctggagcc cgatccaccc gattttccgt
     4681 tcgtcgcact gctggtttcg ggcggccaca cccagttggt ggcggtcacc ggcatcggcc
     4741 gttaccgcct gctgggcgag tcgctcgatg atgccgccgg cgaggccttc gacaagagcg
     4801 cactgctgct cgggctcgac tatccgggtg gcgccgcact cgcccggttg gccgaacagg
     4861 ggcggccggg gcggttccgc ttcccccggc cgatgctcga ccgtccggga ctcgacttca
     4921 gcttcagcgg cctgaagacc gccgtgctgc aggtggtcga gacgcaacgt cgcgccggca
     4981 cgctcgatca acagcagcgg gccgacatcg cccgcagctt cgaggaggca ctggtggaga
     5041 cgctgcgcga gaagtgcgag cgggcgctgg ccgaaaccgg ttaccaccgg ctggtgatcg
     5101 ccggcggcgt cagcgccaat ctgcggctgc gcgccgaact gacggcgatg gccgagcgcc
     5161 gaagtgcgcg attgcatgcc ccacgcctgc gcttttgcac cgacaatggc gcgatgatcg
     5221 cgctggtcgg tgcactgcgg ctcgctgccg gcgaatgcac gccggcgacc atcgagaccc
     5281 agccgcgctg gccgctggag cagctcccac ccatcccccc gctgatccat ggagtcggtt
     5341 gatggacatc gtctacatcg aggatctgcg catcgagacc gtcatcggca tctatgcctg
     5401 ggagcaggag gtcaaacagg tgatcagcat cgacctggag atggccaccg acatccgccg
     5461 tgcggctgcc accgaccgga tcgaggagac cctcgactac aaggcagtgg ccaagcggat
     5521 catcgcctat gtgcagcaga accgccccga gctggtcgag acgctggccg aacgcatcgc
     5581 cgagctgatt cagcgggagt tcgcggtccc ctggttgcgg ctgcggctga gcaagcccgg
     5641 tgcggtgcgc ggttcacgtg cggtgggcat catcatcgag cgtggagagc gcggctgatg
     5701 gcgaaggtct atctcagcat cggcagcaac atcgaccgcg agcaacacat tcgcggctgc
     5761 ctcgatgcgc tggcgcagcg gtttggtccg ctcgagatct cctcggtgtt cgagagcgcc
     5821 gcggtcggct tcagtggtgc ccctttctac aacctggtgg tcggcatcga gaccgagctc
     5881 gatccggcag cgctgaatca acggttgcgt gcgatcgagg aggccgaggg gcgccggcgt
     5941 gatggagcga agttctcggg tcgcacactc gacatcgaca ttctgctcca tggcgaccgg
     6001 cagggtgagt ttgccggcct gcggctgccg cgtccggaga tcaccgaaaa tgcctacgtg
     6061 ctctggccgc tggccgagct ctgcccgacg cggattctgc ccggcagcga ccagtcgatc
     6121 gccatgctct gggccggcta cgatcgggac agtcagccgc tgcacccggt cgatttcagt
     6181 tggcgcggac gcgccatctc gccacgaagc gcttcataga tagagcgaac ggccaccatc
     6241 gacggcgatg atctggccgg tgacataggt tgcctcggcc agaaaatgga ccgcctcggc
     6301 aatctcttcc agtcggccag tgcgccccag cggcgtctgc gccagaatcc gctgctgcgc
     6361 ggcggcctgc tcggccgagg gctgctccgg ccagaggatg gcacccggtg ccacgccgtt
     6421 gacccgcacg tcgggcgcca gctcgcgcgc catgctgcgg gtcagcatcg ccagccccgc
     6481 cttcgagatg gagtaaacgc tgtagccctg cagcggccgc tcggcataga tgtcgatcag
     6541 attgaccacc gcgccgcgcc gcgcccgcag cgtcggcagc agcgcctgca gcagaaagaa
     6601 cggtgccttg aggttgctgt cgaagagcgc ctgccactgc gcctcgctca ccgcgccaag
     6661 cggcgtggga tcaaagctgg aggcgttgtt gaccagcagc ccgacgccgc gctggctggc
     6721 cagctcggcc aactgctgca ctgcctccat ctgacacaga tcagccggca acagctcgac
     6781 cgaccccgcg cgcagcgcgt tcagccgtgc cgccagcgcc tgcgcctcct cggccgagtg
     6841 gcggtaattg agcagaatct gatagccggc gccatgcagc cgctcgacga tggcggcgcc
     6901 gacgcgccgc gccgcgccgg tgaccagtgc caccttcggc accggaatgg gttcgttcat
     6961 gggtgctccc ttgaatcagt cagggtctct cggagctgct cgatcgtggc gagccgtgcc
     7021 tgtcgcaacc gctcaccgat cgccttgcca ctgagcgtgc cgctcaactg gccggcatcg
     7081 atctgacggc aggcggcatg gagccggcgc agccaggccg cctgcggata gcccgactcg
     7141 gcggccagtg ccggccagcg tccggcatcg gcctcggcgg ccagcagaaa ctgctcgaac
     7201 cgttcgggac gacgccaggc atcgagactg tcgagcagtt gcagcagtgc cgcggcatcg
     7261 agccgcagtg cggcatgggc cgtcagcgac tgcgctgcca gcagccgggc cagttgccga
     7321 tggcgattgg ggatgcggca gcggtcgcag atctggtcga tcagtcgcag gttggcggca
     7381 cggtgggccg ctgtgcccgg tggctgctca gggtcgaggt catgcagcag cagcgccagc
     7441 cgcacctccg gctgcgggct caacatcgcg acccgctcca gggcttccag cagaggctcg
     7501 cccccgtcgc actccagccg ctgatcgagt tcgggcagca gccagcccag cgccccgcag
     7561 gcgcgcagca ggtcgatgaa gaggcgcgga cgggcctcgc ccagtgcccg ctccagctcc
     7621 tgccagaccc gctccggcac cagatcggcc agttcgccac tggcactgat ctgccgcatc
     7681 agctccaggg tctcgggcgc cacccgaaat cccagcggtg cgtaacgggc agcaaagcgg
     7741 gccacccgca acacccgcag tggatcctcg gcaaaggccg cagagacatg ccgcagccag
     7801 cgcccggcca gatcacgctg gccaccgtag gggtcgatga tccgtccatt ctcatcttcg
     7861 gccatggcgt tgatggtgag atcgcggcgg cgcagatcgg cctcgagggt cacactgggg
     7921 tcgaagcagg tgacgaagcc ggtgtagccg cgccccgcct tgcgctcggt gcgggccagc
     7981 gcgtactcct ccttgctgcg cggatgcagg aagaccggaa aatccttgcc gaccggacga
     8041 aagccctgcg ccagcagctc ggcaggcgtg gcaccgacca ccacccagtc acgctcggtc
     8101 accggccgcc ccagcagccg gtcgcgcacc gcgccaccca ccagatagat tttcaagcct
     8161 tctcctcttg atcgatccgc tgcctttcat gcctaccggc aaggttatac actgccgggc
     8221 tgttcgatcc caaaaaccat aacagacagg aacaagcgca tgtattacgg ctggcgactg
     8281 ctgatcgcgc tctgtgcgat ctattttctc tccatcggca cggtcttcta cggctttgcc
     8341 gccaccatgc ccgacctgat ccaggccttc ggctggagcc gcagtcaggt cagtgccggc
     8401 ttctcgatcg ccagcgccac gctcgggctc tgcggtccgc tgatcgcctg gatgattcgc
     8461 cgcttcggca cccgcatgac catcttcatg ggcggctttt cgatcctgat cggcgcgctg
     8521 ctgacctaca ccacccagac ggtgccgcaa ttctatctct cctgtctgtt tctcggactg
     8581 ggtctggcga tgcagaccat catccccggc acccagctga tctccaactg gttcgcccgg
     8641 cgccgtgcgc tggccatcgg gctgttcatg gcggcgggtg gcgtcggcag cggtgtgctg
     8701 accccgctgt tcgccttgct ggtcaagcag accggcgact ggcggatcgt ctggctgatc
     8761 atgaccggcg ccggcatggg ttcgtcactg ctggcactgt gggtggtcaa ggagatgccg
     8821 gccgatgtcg gacagcaggt cgacggcgtt tcggaaacaa gcagcggcag cgccaaaccg
     8881 gtcaaacagg tccgggtcta tcagaccagc cacccgtggg agaccaaggc agccttctcg
     8941 accgtcgtgc tgtggctcgc cattctctgt gcctcgatgg cctcgctcgg cggtggtctg
     9001 gtcaacaccc aggcgctgct gcacctgcag gatctgggac acgagaaggt gctggccgcc
     9061 tcctcgatcg ggctggtcgg cttcctcagc gtggccggcc gggtcttctc cggcaccctc
     9121 ggtgactaca tcgaacccaa gtacatgctg agcgccgggc tgctgatctc gctggtcggc
     9181 atctacctgc tcaacattgc ccatgagccg ctgctggtct atctgttcgc catcctgttc
     9241 ggcctcggca acggtctggc ggtggtgacc agcccgatga tcatcgccaa ctacttcggc
     9301 agcaagaact acgcctcgct ctttgcggtg cgcagcatga tcgtcaccat catcgcggcg
     9361 ctcggtccgg tgctggccgg cgcctggttc gacaaattca acagctacag ccaggtgttc
     9421 tggttctaca tcggcatgtg cctgatcgcc ggcatcgccg ccctgatgat gcgaccgcca
     9481 attccggccg cacaggcggt ggcgacatcc tgatcggacc aaacgacgcc cgcatggaca
     9541 aggccaccga acagccgctg cgcctcccgg caccctgcgg ggtgctggag gtgcgctacg
     9601 cggcgccgac ggcaggccgg ccggtggcgg tgatctgcca ccctcacccg ctgttcggcg
     9661 gttcgctcga caacaaggtg gtcttcacgc tgatgcgcag ctttcagcaa ctgggtctag
     9721 gcaccctgcg cttcaacttt cgcggtgtcg gtgccagttc cggccattac gacgacgggc
     9781 gcggcgaact cgacgacctc gccgcgctct gccgctggct ggctcagcag ggcaacgccc
     9841 gactctggtt ggccggtttt tcgtttggcg cctacatcgc cgcacggggt tgccaggcgg
     9901 cagcggactg gggtgtgacg gtggagcagt tgattctggt cgccccgccg gtcgggcgct
     9961 tcgatttcag ccccctgcag cgcttcgact gcccggtcac gctgttgcag ggtgaggccg
    10021 acgaactggt caccgccgac ggtgtcgctg cctgggccga gcaactgcac agcccgcacc
    10081 agttgctgcg ctttgccgat tgctctcatt tttttcatgg acagttggta gaattgcgca
    10141 gccgcctggt cgcggcactc gccccaaccc tggtgaccgc ctgactcttt cggctgacgc
    10201 tgttgtccat tcccgccctc ctgccctcga tctccccgct cgaacgctac cgcgcctcgc
    10261 tggcgcagcc cggtttcgag catgatccgg cacaggagca ggccgtgcgc cacctgcagc
    10321 ggctctacga tgagctggtg gtgacgcagc cgcaatccac tggcctgctc ggtcggttga
    10381 gccgaccctt ccgcaagccg ccgacgccga tcaagggact ctacttctgg ggcggtgtcg
    10441 gccgcggcaa gacctacctg atggatctgc tgttcgatgc cgtgccgatc gccgagaaag
    10501 cacgcatgca ctttcaccgc ttcatgcgcg aggtgcatgc acagttgcag gcgcggcagg
    10561 gcgagagcga cccgctgcgc ggcgtggccc aagcctggtc gagccgcatc cggctgctct
    10621 gtttcgatga gttcttcgtc agcgacatca ccgatgcgat gctgctcggc acgctgctgg
    10681 agcatctgtt caaccagggg gtcatcctgg tgaccacctc caacatccac cccgatggcc
    10741 tctatccgaa cggcctgcag cggtcgcgtt ttctgcccgc catcgcgctg ctgaaggcgc
    10801 acaccgaggt ggtcaatgtc gatggcggca tcgaccatcg tctgcgcacg ctgacccgcg
    10861 caccgctcta ctacagcccg ctcggtcagg cggccgatga caatctgatg gcctgctttc
    10921 aaagactcgg ctcgacgacg attgaggccg atgcggtgat gaccatcgac aaccggccga
    10981 tccagacgcg ctatctggtg gacgatctgg cctggttcga cttcgaccag ctctgcgacg
    11041 gtccgcgcag ccagcgtgac tacatcgaac tggccgagct gttcgagacc gtggtgatca
    11101 gcaacatccc gctgctgacc acccatcatg aggaccagag ccgccgcttc atcaacctgg
    11161 tcgatgagtt ctacgactgc ggcgtcaagc tgattctgtc ggcggccgca ccgcttgccg
    11221 agctgtatca gggcgagcgg ctgcgcttcg agttcgaacg cacccgcagc cgcctgctgg
    11281 agatgcagtc gcacgactat ctggccaggg cacaccggcg cacgaccggg gggccgtgaa
    11341 gccggcgctg tcgcgtcgca ttcaatggtt cagaagatca gccagcgcac agcctgcagc
    11401 gccagccagg cgtaacccgc cgccagcagg tcatcgaaca tgatgcccca gccgcccttg
    11461 atccgccggt cggcccaacg caccggccag ggcttggtga tgtcgaacag ccggaacagc
    11521 gcaaaaccga gcagcagcca gagcggttcg gccggtaccc ccagtagcac cacaccgatg
    11581 gccgccatct cgtcacagac gatggccgga tggtcatcga cgccgagtcg gcgggtcgcc
    11641 gtggcggcgg cccagacgcc gagcacgatc gcagcaccca gcagcagcag gtagagcccc
    11701 agcggcgcgg ccgagctgag ccagccccac gccggcagcg ccagcgccgt gccgaccgtg
    11761 cccggcgccc agcgggagag ccccgcgcca aagccgagcg cccaagcatc gagcgggctc
    11821 gccgtcagct gtttccagcc cggtttcggg cgcatcactg cggcgcctcg aagtggcggt
    11881 aaccatgcag cgccacatcg gccggctgct gctgaccgtc gagagtcagg gtcaatccgg
    11941 ctgctgcagt cgccagcccg atccgtcgac agccgacgcc caccgtggcc agcgcggcct
    12001 cgatgcgcgc ctcatgcgtg cgcggcgccg tgaagcagag ctcatagtcg tcaccatgac
    12061 cgaaggccca gcggcgggcg gtggcgcgat cggccacccg acacagcgcg gccgacagcg
    12121 gcagatcgtt cagttcgagc gtgaaaccga cgccgctgcc gctgatcaga tggtgcagat
    12181 caccggccag accatccgac aggtcgatca tcgcgctggc gagcccgcgc agttgcaagc
    12241 cggcctcgat gcgcggcgtc ggttgcagat agcgctggct caggaactca cggtccgtcg
    12301 cgtcggcctg caactggccg cgcagcagcg ccaatccggc cgccgcatca cccagggtac
    12361 cggtgacata gaggccatcg cccacccggg caccgcgccg tgacagcgcc agcgccgctg
    12421 gcacctcgcc ggccacctgc agcgtgatgc tgcgcggacc ttgcgccaga ttgccgccga
    12481 ccagcaggct cccgtagcgc actgcgcagg cggtcaggcc acggctgaag ccatcgagcc
    12541 agaactcgtc gaccaccggc agtaccagcg ccagggtgaa ccagctcgga gaggcggcaa
    12601 cggcggccag atcactcagg ttgaccgcca gcgcacgcca gccgatcgaa aaggggtcgg
    12661 cgtctgccag aaagtgcacc ccctcgacca gggtgtcgat cgagatggcg agtgatgcat
    12721 cgcgcggcgg gtcgagcagg gcgcagtcgt cgccgatacc ccagcgcacg ccggcgcagg
    12781 gcgcaactcc gaccgccgcc agcggcggct ggctgaagta gcgttcgatc agttggaatt
    12841 cgtgcagcgc catgcggcat gccctcgccc gccgtcagcg ccgccgggcc tcgacttccg
    12901 ggcggcgaaa ctgcgctgcg atccgatcga gcacgccatt gacatatttg tggctgtccg
    12961 gcgcggcaaa ggatttggcc agttcgatcg cctcgttgat caccaccgga tagggcagct
    13021 cgatgcagtg catcaactcg tagctgccaa tcagcagaat cgcccgttcg accggggtga
    13081 gctgttcgag cggccggtcg agcagcggcg ccagcacctg atcgagctcg ccatgcagcg
    13141 cgatgatgcg ccgcagcagt tcggagaagt agtcgagatc ggcctgcctg accggctgct
    13201 cttcacgaaa ctgccggtcg atctcggccg cgctggcgcc ggccagctgc cactgataga
    13261 gcgcctgcag cagcagccgg cgcgcctgat ggcgcgcctt cgacttcggc ctgaccgcgg
    13321 cgggtggggg cgtgtcgctc atcgcgctca tcgtgccggt tgcaactggc gcagcaggct
    13381 caccatctcg aaggcgcaga gcgccgcctc ggcccccttg ttgccggcct tggtgccggc
    13441 ccgctcgatc gcctgctcca gcgtatcgac cgtcagcacg ccgaaggcca ccggcaggtt
    13501 gctcgacagg ctcacctgcc ccagcccctt gacgcactcg ccggcaacat agtcgaagtg
    13561 gggcgtgcca ccgcgaatca ccgcgccaag cgcgacgatg gcatcgaagc ggccactctc
    13621 ggccgcctgc ctgaccacca gcggcagctc gaacgcgccg ggcgcgcgga tcagcgtgat
    13681 ctgcccggcc ggaatgccat ggcgcagcag gacatcggcg gcgccggcga tcaggctctc
    13741 gacgatgaag gcattgaagc ggctggcgac cagcgcgaag cgggcatcac gggcggtgaa
    13801 gtcgccactg atctgttgaa actggctcat gttctgcggt tactccaggt ggatgctgcg
    13861 gccatcggcg gccagatact cgaccacggt caggtcgaat ccggagatgg cattgtattt
    13921 gatcggcgag ctcatcaggc gcatctggcg cacgcccaga tcgcgcagga tctgcgagcc
    13981 ggtgccgacg gtgtagaaaa cgttggggtt ctgctcgacc cggcgccgct gcggtaccgc
    14041 ttcgagccgc gccaggctgg ccaatgtggc gtcgggtggc tcgttgccgg cgatcagtac
    14101 cagcaccccc tgtccggccg cggcgatggc cgccagcgcg tcatggaccg accaactggc
    14161 ggtcgagctg gcgatctgca acaggtcgcg cagccggtcg gcctgctgca cccgcaccag
    14221 caccggctgg tcggggtcga tctcgccaca gaccagcgcc agatggatgt caccctgcag
    14281 ggtgtcgcgg taactgacca gcgtgaaggg accgtggcgg gtcatcacct cgcggctgcc
    14341 gagacggatg atggtcttct catgcaggat gcggtagtgg atcaggctgg cgatggtgcc
    14401 gatcttgagg ttgtgggtgg ccgcgaactg ctccagctgt ggccggcgcg ccatgctgcc
    14461 atcctcgttc atgatctcca cgatcaccgc cgccggctcc agcccggcca gccgtgccag
    14521 atcacagccg gcctcggtgt ggccggcccg gctcagcacg ccaccctcgc gggccaccag
    14581 cggaaaaatg tggcccggct gcaccagatc ctccgccacg gcatcggctg ccaccgccac
    14641 ctggatggtg cgggcacggt cggcggcaga gatgccggtg gtcacccctt cggccgcctc
    14701 gatcgacgcg gtgaaattgg tcgcgtgcgc ggtgcggttg tcgctgacca ttggtccgag
    14761 ccgcagccgc tgggcgcgcg cctcggtcag cgtcaggcag accagcccgc aggcatggcg
    14821 caccatgaag ttgacatcct ccggccgcac cttgctggcg gccatgatca ggtcaccttc
    14881 gttctcgcgg tcttcgtcat ccatcaggat caccatgcga ccctggcgaa tctcctcgat
    14941 caactcttcg atgctgttca attccatgcc atcttctctt gattcagcct gatacggtgg
    15001 gcgtcaccag cccggcctgc tccagcagcc ggctggtcag cggtgccgcg ctgccctcgg
    15061 cggcctgctc gcccagcagc agccgttcga gatagcgggc cagcaggtcg acctccagat
    15121 tgaccggctg accgggctgc cagccgccga gagtggtggc agtgagcgtg tgcgggacga
    15181 tgttgagctc gaactccgcc ccctcgaccc ggttgacggt gaggctgacc ccctcgacgg
    15241 tgatcgaccc cttggcggcg atgtagcggg ccagctcacg cggagcgcgc aggcgcagca
    15301 ggcgcgagcg gccggccggc gccagcgaca gcagctcgcc gacaccatcg acatggccgc
    15361 tgaccaggtg cccgcccagc ggcgtcgcca gcgtcagcgc cttttcgagg ttgaccggcg
    15421 atcccgcccg cagcccctgc agcgtggtgc ggttgagcgt ctcgatcgac agatcggcgc
    15481 tgaagcggtc ggcactcagt tcgaccgcgg tgaggcagac accattgacg gcgatgctgt
    15541 cgcccagtgc ggccgtgcgc agatcgagcg ctccggcctc gaccgtcagg cggtagtcac
    15601 cgccgacggg ccgcagtgcc gccaccgtgc cgatggcctg gatgataccg gtaaacatct
    15661 cactcctggg tggcgtgtgc cgcatggtcg aaacgggcgc gaatgcgcca atcatcgccg
    15721 agcgggcgga tttcgtcgat caaaagcggc caacggtcgg ccatccgctc gatggccggc
    15781 aactgcaaca gcggccgggc ggcactgccc agcagcagcg gcgccaggta gatccacagc
    15841 tcatccacca acccctgttg caccaggttg ccggccagcc tggggccgca ctcggccagc
    15901 agctcattgc actgcaacgc cgcgagccgt tgcagcaccg catcgagatc gaccccctgt
    15961 ccggcggttg ccgcgatcgc ctcgatgcgc acatgtgcgc ggtcgcgcaa ctgcgctgcc
    16021 cgtgcctcat ccagtggctg ccgggtgaag atcagcgtct cgcccgcctc gtgcagcagc
    16081 cgtgcgccgg gtggcgtgcg cagttgcgaa tcgaccacca cccgcaacgg ctggcgcagc
    16141 ggccgctcgg cccactcgcc cagcgcatcg aggtcacgca ccgtcagtgc cggatcgtcg
    16201 gccaataccg tgccactgcc ggtcaggatt gccgaactgc gcgcccgcag ccgctgcacg
    16261 tcggcccgtg ccgccggccc ggtgatccac tggctgacgc cattgtgcag cgccgtgcgt
    16321 ccatccagac tcatcgccag cttgagccgc agcagcggac gcccgccacc catccgccgg
    16381 atgaaaccgg agttgagcgc agccgccgcc tccgccagca ggccgcactc caccgcgatg
    16441 ccggcgcgcc gcagggcggc caggccgccg cccgccaccc gcggattggg gtcttccatc
    16501 gccgccacca cccgcgccac gccggcggca atcagcgcct cgctgcaggg tggcgtgcgg
    16561 ccatggtgat tgcagggctc cagcgtcaca taggcggtgg cgccgcgcgc ggcggctccg
    16621 gccatccgca gcgcctcgac ctcggcatgg ggttcgccgg cgcggtgatg ccagccttgg
    16681 ccaaccaccg caccctgcct gaccagcaga cagcccaccc gggggttggg gtcggtgcca
    16741 tagagaccac gctgcgccag ttgcagcgcc tcggccatga aacggcgatc ctgctcgctc
    16801 tcggtcaagg cggcactctc ctgcgctgga cgcacgctga tgaagtgggg gcggcctcag
    16861 ccctcaccgc cacccttctg ctccagccgc tcgatctccc gacggaattc atcgagatgc
    16921 tgaaagcggc gatagaccga ggcgaagcgg acataggcca cctcgtcgag ctgctgcaac
    16981 tgcgccatca ccgcctcgcc gaccagatcg gaggcgacct cgcgctcgcc ggtggcgcgc
    17041 aattgatgct tgatgcggtt cagcgcggcc tcgacatcct cgacactgac cggccgcttt
    17101 tcgatcgcgc gcagaatgcc gctgcgcagc ttctcttcat tgaagggctc gcgctggcca
    17161 ttgcgcttga tcaccttggg catcaccagc tcggccagct cgaaggtggt gaagcgctcc
    17221 tgacaggcag tgcattcgcg ccgccgccgc acctggtccc cgcccgcgac cagccgggag
    17281 tcgatcaccc gggtctcttc ggttccgcag aacgggcagt gcatcgcgac ggcaccggca
    17341 cgcttcagcc gtagaccggg tggcgcgcgc agatctcgct gacctgctgc cggatgcgct
    17401 ggcaggtggc atcgttgccg aggtcatcga gcaggtcgca gatccagtgg gtcaactgct
    17461 ccacctcgcc gctgtcgaag ccgcgccggg tcaccgccgg tgtaccgatg cggatgccgc
    17521 tggtgacgaa gggcgaacgt ggatcgttcg gcaccgcgtt cttgttgacg gtgatgtgcg
    17581 cccggccgag tgcctcttcg gcctccttgc cggtgatgcg cttgtcggtc agatcgagca
    17641 ggaacaggtg gttgtcggtg ccgccggaga cgatgcgata gccgcgggcg atgaactgcg
    17701 ccgccatcgc gcgggcattg gccagcgtct gctgctggta gagcttgaac tccgggctca
    17761 tcgcctcctt gaagcagacc gccttggcgg cgatcacatg catcagcggt cccccctgag
    17821 tgccgggaaa gactgccgaa ttgagcttct tctcgatctc cggattggcc ttggccagaa
    17881 tcagcccgct gcgcgggccg gccagcgtct tgtgggtggt cgaggtggtg acatcggcaa
    17941 tgccgaccgg gctggggtag gcgccgaccg ccaccagacc ggcgacatgg gccatgtcgg
    18001 ccaccagaaa ggcccccacg ctgtcggcga tggcgcgaaa gcgctgccag tcgaccagcc
    18061 gcgaataggc cgaaaagccg gtcatgatca tcttcggccg atgggttcgt gccagcgtct
    18121 cgacctgatc gtagtcgatg cggccgctgg ccgggtcgag cccgtagctg accgagtggt
    18181 agattttgcc cgagaagttg accttggcac cgtgggtgag gtggccgccg gcggccagat
    18241 ccatgccgag taccgtgtcg cccggatcga gcagcgccat gtagaccgcc gcgttggcct
    18301 gcgaaccgga gtgcggctgc acattgacat agtcggcacc gaacagtgcc ttggcccggt
    18361 cgatcgccaa ctgctcgacc tggtcgacat actcgcagcc accgtaataa cgcttaccgg
    18421 gatagccctc tgcatacttg ttggtcagca ccgagccctg cgccgccagc accagcgggc
    18481 tggtgtagtt ttccgatgca atcagttcga tatgcgcctc ttgccgccgg ctctcgccct
    18541 gcatggcggc ccacagatcg ctgtcgaagt cggcaagggt caggttgcgg tcaaacatcg
    18601 gcacctcgaa cacggacaaa aagaaaaaag gccggccatt gtactccagc cggtcacaaa
    18661 agcgctcttg cgcctgctag gctttgcaat gcgcaccgcc actttcggaa ccctcggaat
    18721 ggaccagcca ccgacccgcc ttgccggaga gatcttccac cacactgacc ggcgtcgggt
    18781 gtcacgcatc cgctgctacg ctccagtgct ggtcgaaagc acggccggct gctacccggc
    18841 cgcactggcc gacctctcgc tgcaaggcgc cctgctgcag gtgccgcagt ggcaaccggc
    18901 cgtcaacgaa tgcgccacgc tgctggtcga gctcgacact cagggcatcc agctgattct
    18961 cgaagcgcgg gtgcgccatc agcacgacgc ccgcattggc gtgcagttca ccgaagtcaa
    19021 cgacctccag cagcgctgcc tgcggcagtt cctgttccgt ggcacggcgg atcaaaagca
    19081 gctccctcga acatggcgca acctgttcgg tcgcggtcca atccggcagc ccccgcagta
    19141 acacagtaaa aggccacacg cttgcgctac cgaggcgtct tgggcaaact tgccggacgc
    19201 ctgcttgaat ggcggagttt tcggccccgc accgggttcg ccagggtgca gccccctctc
    19261 aacttctgaa aataaccaaa ggtcgatcat gagaaactac gacaagctct atatcaacgg
    19321 tgagtgggtc gcaccccatg gcaaggggag cagtcaagtc accaacccct tcaacaacga
    19381 agtcatcggc agcgtgccgc agggtgacct gcaggatgtc gaccgcgccg tgcaggccgc
    19441 caaggccgcc tttccggcct gggcggccac cagcgtcgaa gagcgcgccc agtacctcga
    19501 gaaactggtc ggcgccatgt ccaagcgcgc caaggagctc agctcggtga tctcccagga
    19561 gctgggcatg ccgatcagcc aggccgagat gatccaggtc ggcatggcca tcggcaccct
    19621 caacagcttc ccggccctgc tgcgcagcta caagttcgaa aagcagatca acaactcgac
    19681 ggtcgtgcgt gagccgatcg gcgtctgcgg cttcatcacc ccgtggaact acccgctcta
    19741 tctgatcgtc gccaaggccg cccccgcgct ggccgccggc tgcaccatgg tgctgaagcc
    19801 gagccgcgaa accccgctca acgccttcat cttcgccgaa ctgatcaacg aagtcggcct
    19861 gccgcccggc gtcttcaacc tggtcagcgg ccctggtggc gtggtcggtg aagggctggc
    19921 cgcccacccc gacgtcgaca tggtctccat caccggctcg accgaagccg gcatcagcgt
    19981 cgccaaatcc gccgccaaca ccgtcaagcg cgtcgcgcag gagctgggtg gcaaatcggc
    20041 caacatcatc ctcgacgatg ccgacctcaa caccgccgtc gccgcgggtg tgcgcggcgt
    20101 gatgatgaac tgcggccaga cctgcgccgc actgacccgc atgctggtcc ccgaaagccg
    20161 caaggacgaa gtcatcgcca tcgccaaggc caccgccgaa tcgatcaccc aggatgatcc
    20221 ttccaaagag ggcttccaca tggggccgct ctcctcgctg cagcaggcca acacggtccg
    20281 caaatacatc cgcaagggga tcgaagaggg tgccaccctg gtcaccggcg gcctcgaaga
    20341 gccagcgggt ttcgaaggcg gcgccttcgt caagccgacc atcttcgccg atgtgaagaa
    20401 cagcatgacc atcgcccagg aagagatctt cggaccggtg ctcagcatcc tcacctacaa
    20461 ggatgaggcc gatgccatcc gcatcgccaa cgacaccgtc tatggcctct ccggctcggt
    20521 ctggtcttcc gaccccaacc gcgcgctgcg cgttgccaag caactgcgca ccggccaggt
    20581 ctccatcaat ggtggcggca tgaacctgat ggcgccgttc ggcggctaca agcagtcggg
    20641 caacggccgc gaactgggcg agttcggtct cgaagagtac ctcgagatca aatcggtgca
    20701 gatgccgccg gccgctgcgg ccagctgagt cgcgcgctcc agaccgcaaa ccggtctgga
    20761 gcggcagtca aaaaacccgt cgccggcgtg ccggcggcgg gtttttcatt ggagctcatc
    20821 cgcgctggtc gctcggtgca aaaatcacta ccaaggagcg gggaagaacc agcgcgtggc
    20881 cgcggcctga cggcctttgc ccaccctgtc tctggctgcg ctcagccacc aaaggcatgg
    20941 cgcagcacga tggtctcgtt gcggtcgggg ccggtagaga tgatgtcgat cggcgcgcca
    21001 accagtgcct cgatgcggtc gaggtaggcg cgggcattgg ccggcaactc ttgcaggcgg
    21061 gtgaggccga gggtcgattc gctccagccc ggcagctctt cgtagatcgg caccacctga
    21121 tcatagccct cgctgtcgag cggaatcggc agcggctggc cactggcatc ctggtagccg
    21181 atgcagacgc ggatcagatc gagcccatcc agcacatcga gcttggtgat gcacaggccc
    21241 gagaggctgt tgagttcgac cgagcggcgc aggatcaccg cgtcgaacca gccgcagcgg
    21301 cgggcgcgac cggtggtgga accgaactcg tggccgcgct tggcgaggtg ggcgccggtg
    21361 gcgtcgaaca gttcggtggg aaaggggccg gagccgaccc gggtggtgta ggccttggtg
    21421 atgccgagca catggtcgaa ctgcaacggg cccatgccgc tgccggtggc ggcgccgccg
    21481 gcggtggtgt tggaggaggt gacgaaggga taggtgccgt ggtcgatgtc gagcagcgcg
    21541 ccctgcgcgc cctcgaacag caggttggca ccatcacggc gcagccgatg caacagaccg
    21601 gtgacatcgg tcaccatcgg ccgcaactgc tcggccagcg ccaggttgtg ctccagcgtc
    21661 tgctgaaagt cgatcggcgc ctgctggtga taccgttcga gaatgaagtt gtggtagtcg
    21721 agcacgtcgc gcagcttgct ggcaaagcgt tcgggctcga acaggtcacc caggcgcagg
    21781 ccgcggcgcg ccgccttgtc ttcgtaggcc gggccgatgc cgcgaccggt ggtgccgatc
    21841 ttggcggcgc cgcgcgcctg ctcccgtgcc tggtcgagcg ccacatgcga cggcaggatc
    21901 agcgggcagg cggcgctgat gcgcagccgc tcgcgcaccg gcaccttgcg cgcttcgagc
    21961 atggcgatct cctcgaacag cgccactggc gaaaccacga cgccgttgcc gatcacacag
    22021 gtgacgccct cacgcaggat gcccgacgga atcaggtgca gcacggtctg cgcgccatcg
    22081 atcaccagcg tgtggccggc attgtggcca ccctggaacc gcaccaccgc ggcggcctgg
    22141 tcggtgagca gatcgacgat cttgcccttg ccctcatcac cccactgggt tcccagaatg
    22201 atgacgttct tgcccatgct tgctctctcc gctgcgctgg cgcgccgttg ttggtttgat
    22261 tgggttggta tcacagctcg accagtcgcc actggccccc ggcgctgaac agcagttgcc
    22321 ggtcacagcc gaggtcgcca acgctgccgg agtcgcccgg cagggccgaa atcacccgtt
    22381 cgccggcggc gcgcaactgg tcgatgacgg cgcggtgggc cggctcatcg ctcggctgcg
    22441 gtacaaagat cgccccaggc accgggtcgg cgggtcggcg cagctcaaag agcgacttga
    22501 ggtcggtgct gaaaccggtg gccggtctgg cgcggccaaa ggcttcgccg atgtggtcat
    22561 agcggccgcc cttggccact tcgcggccgt ggtcactggt gtaggcggca aagaccaggc
    22621 cggtgtggta gtggtaaccg cgcagttcac tgaggtcgca gtagagcgcc accgagggat
    22681 agagccgacc gatgcgacgc accacctgct ccaggtcgtc cagtgcctgc tgcaccggtg
    22741 caggcgtggc agccagcagc tggcggctgc gtggcagcac cagcccgggt tcgccatgca
    22801 gatgcggcag ctcacgcagc agtggcgccg gcaccggctg ccggtcgagc agttgatcga
    22861 tctcggtgca ggccttggcc tgcatcagct cgaacagctc ctgctggatg gcgggcgcaa
    22921 gctggctgga ttcggccaaa gtgcggtaga tcgccacatg gccgaggtcg agactgaggt
    22981 cggtcaggcc gatcttcatc agcccttcga gcatcagcgc gatcacctcc agatcgctgt
    23041 cgatgccggc atggccatag agctcggcgc cgacctggat cggaccacgg ctgtcgaagg
    23101 gctggtcggt gcgggtgtgc agcacgctgc cggcatagca gagccgcacc ggaccattgc
    23161 gccgcagcgc atgggcgtcg atgcgcgcca cctgcggggt gatgtcggca cggatgccca
    23221 tcatccgccc ggtcagctgg tcggtgacct tgaaggtttt caggtcgagg tcatggccgg
    23281 cgccgatcag cagcgattcg aggtattcga tgaagggcgg catcaccagc tcgtagcccc
    23341 aacttcggta gaggtcgagc agttgccggc gcagctgctc gtgcacctgg gcatcgggcg
    23401 gcagaatctc ttcgatgcca tccggcagca gccagcgatc ggcaatggtc atggcgttgg
    23461 ctctcgggcc gcgaccgtgg cgcctgccac acccgcgacc ggtgtcagtg gttgatcaga
    23521 tggagcagcc cggctccggt cagcatgctg ccgaggccca gcaggcggat ggcgcgccgg
    23581 tcgagccggg cgatctgctg gagcagtccg cgccactgtt cgggcgcgac aaaggggagt
    23641 atcccctcca gcaccagcag cagacagaga gcgaccagca gctcatgcca tccgacgccg
    23701 gtcaccccgc tgcccagccg tagcgcgaca aaacgtcagg caacggcgtt cagcgcgccc
    23761 gcggagcgcc ttcgcgctcc ttgaagtatt tgaagaaatc gctggtcggc gagagcacca
    23821 gcagatcctt gcggtcgctg aagctcttgc tgtaggcatt gatgctgcgg tagaagccgt
    23881 agaactccgg atcgaccttg tagacggcgg cataggtggc agaggcgacg gcgtcaccct
    23941 cgccgcgcaa cttctcggca tcacggtagg cattggccga gatgaccgct ttctcgcggt
    24001 cggcctgggc ccgcagcatc tcggcctgct ccttgccacg cgagcggtac tcacgcgcca
    24061 gccgctcgcg ctcactcttc atccggtcgt agaccgagct gctgacatcc tcgggcaggt
    24121 cgatgcgctt gatgcggatg tcgaccagtt cgatgcccag ctcgccacgg atgatgttgc
    24181 cgagcttgac gatcagatcc ttcatcagct gctctcgttc gctgctgacc acatcatgca
    24241 tggcgcgctc gccgacctgg tcacgcaggc cgttgttgac ccgctgcgcc agcaggcggg
    24301 cggcctggga ctcgtcgccc gaggtggcgg tgtagtaggt ctgcacatcg gcgatgcgat
    24361 acatcacata ggagtcgatg atcagcgcct ttttctccag cgtgaagaag cgctggggct
    24421 gggcatcgat ggcggtggtg cgggcgtcga acttcttcac ctcattgatg aagggcttct
    24481 tcaggtgcag cccgggctgg atgtcggcct gcaccacgcg gccgaattcg agcagcaccg
    24541 cacgctcggt ctcgctgacg gtgtagaggg cctggtaacc cgccaaggtg agcagtgcgc
    24601 ccagcagcag ggccaacatg gatcgattgc ccatctagcg actctccctt gaacggctgc
    24661 tgttctgccg gttgcgcagc tcctcgatga cacggtcggt gagccggctg acgtcaacgc
    24721 cgctgtcggc ggtgaaggcg ggtgtcgtcg aaaccggcgg cagggtggag gttgtcgccg
    24781 cgccagccag ccggtcgagt ggcaggtaga tcatgttgtt gccgccactc tggtcgatca
    24841 gcaccttgct gctgttgccc agcaccgact cgatggcatc gatgtagagc cgctgacggg
    24901 tcagctcggg ggctttctga tactcggcca ccatgctctg gaagcgcagc gcatcaccct
    24961 gggcacgggc gatcaccccc tccttgtaac cctcggcctc ctgaatctgc cgctgcgcat
    25021 agccgcgcgc ctcgggcacg atgccgttgg cataggcctc ggcctcgttg cggaaacgtg
    25081 cttcatcctc gcgcgccttg atcacgtcat cgaaggcggc ctgcacctcc ttcggtggtt
    25141 tggcctcctc gatgttgacc ttgctgatct ggatgccgct ctgatagagg ttgaggtact
    25201 cctgcatctt gctgtgcaca tcgatgccga tctgctcgcg cccttcggag atcacccggt
    25261 ccatcttgga ggtgccgacg acatggcgca gcgcactctc ggtggcgttt tcgagcgccg
    25321 actcgggatc acgcacccgc agcagaaaat ccttgggatc actcaccaca tactgcacgg
    25381 tgacgctgac gtcgacgatg ttctcatctt cggtcagcat caggctgcgg tggttgcggt
    25441 cgcgcacccg ggtcacgttg accttgtaga gcgcatcgat cagcggaatc ttccagtgca
    25501 ggccgggcat gacgctgctt tcgtaacggc cgaaccgcag cacgatgccg cgctcctgct
    25561 gattgatggt gtagagcgcg ttgtaaccca gcaggaacag caacagaacc agcagcccca
    25621 gcagcaggcc acccatgccg ctgccgccat cgccctgagc ggtgccgccg cccttgccgc
    25681 cgccacccag caggccaccg agcgtggcgc ccagcttgcg cagcagttcg tcgagatcag
    25741 gcggcccctg atgctgattg ccacggttgt tgccccaggg atccttgggc tcaccgccgc
    25801 cgggctcgtt ccacgccatt gctgttccgc ctgaccgttc ttgaagagaa tgcgttgaaa
    25861 agcggcgatt ctaggggggt gcaggcgatg ccgcaagctg gcgcagcctc tgtgagcccc
    25921 ccagcgcggc cgggtcgatc ccctcacgcg ccagcaggcg gctcagcgcc tgaccggaga
    25981 agcgaaccgc gacccgcgaa atgccgctct cgtcggtggt ttcgccaaac accgcgccca
    26041 gtgcatagag ccgggcgcgc aggcgaccga gactgggagg cagtcgcagc tcctcctcga
    26101 cccagtcggg ctgcagcagt tcggccagcg cctgtcgcag cagatcgagg ccggcgccgc
    26161 tgcgggccga gagccagacc cgcgtcggcc gcccctggct gtcgcgctcc agacggggcg
    26221 acaggccatc gaccagatcg atcttgttgc agattcgcag ccgcggcaca gtgtcggcac
    26281 cgatctcggc cagcaccgcc tcgacctcct ggctgcgctc ctgctggtcg gcgtcggcag
    26341 agtcgatcac ctgcagcagc aggtcggcct cgcggctctc ttcgagggtg gcgcgaaagg
    26401 cttcgatcaa ctggtgcggc aactgacgga tgaaaccgac ggtgtcggcc aggatcgccg
    26461 gtcccagatg gggcaactcg atgcggcgca atgtggagtc gagcgtggcg aagagctggt
    26521 cggcggcgaa ggtgtcggca ccggtgaggg cgttgaacag ggtcgacttg ccgctgttgg
    26581 tatagccgac cagcgacagc accggaatct cgccacggcg ccgggcgcgc cgcccctcgg
    26641 cacggcgctt gcgcaccgct tcgagccgct gctccacccg ctgcgtgcgc tgccgcagca
    26701 tccgctggtc gagctcgagc tgggtctcac cggcaccgcg cagaccaacg ccgcctttct
    26761 gccggtcgag gtgactccag ccgcgcacca gccggctgga gaggtgctgc aactgcgcca
    26821 gctcgacctg caactgcccc tcatgggtgc gggcgcgcag cgcgaaaatg atcaggatca
    26881 gcccggtgcg gtcgagcacc cgccgtccga gccggcgttc gaggttgcgc tcctgcgatg
    26941 atgaaagctc atggttgaac agaacgatac cggcatcgaa ctgcagggca gcggcggcca
    27001 gttcatcgac cttgccgctg ccgagaaagt ggccgggggc cggtttggct cgactggcgc
    27061 ggaagcggag caccggttcg aggccggcag agcgggccag ttcatgcagt tcgtccaggt
    27121 cggcgctgtc gccgggcagg tcgagctgaa ccaggatggc gcgctggccg atctcgggac
    27181 ggtcgaagaa ctgaagcgaa gcgatcgggg cgattactcg ttgcctcctt cgtagggctc
    27241 ctcatcctcc atgtcggcat tgccggccgg cggcagccgc accggacgtg ccggcaccac
    27301 cgtggagatg gcatgcttgt agaccatctg gctgacggtg ttcttcaaca gcacgacaaa
    27361 ctggtcgaag gattcgatct gtccctgcaa cttgatgccg ttgaccagat agatcgaaac
    27421 cggaatccgc tccttgcgca gcacgttgag ataagggtct tgtagcgagt gccccttaga
    27481 catggcaggt ctccttgatg gtaatgctcg aattgtactg caaatttccc aactcgacag
    27541 gcgcgcggct gcggatctgt tcaccgcctc gaaatgggta acggtgtcac gattttcaag
    27601 gccagcgcca gctgctcggc gcgctccagc ggcagcggcc gcagtccggg ccagccacgc
    27661 atccaggtca actgccgctt ggcaagctgc ccggtggcga tcagggaccg ttcgatcatc
    27721 tcggcatggc tgctctgccc ggccagatgg ctccagacct gccggtaacc gaccgcacgg
    27781 atcgccggca ggtcggggtg aagatcgccg cgctcgaaca gtcgctgcac ctcttcgacg
    27841 aagcccatgt cgagcatctg ccgaaagcgc tgcgcgatcg gcccgctcag caactggcgg
    27901 ctctgcggct ggatgccgag ggtgacccac cgctgtggca gcggcgccgc ctgctgttcg
    27961 gcatggtgct gactgagcgg aatgccggtg tcgatgaaca cctccagcgc ccggatcagc
    28021 cgctggcggt tgccgagcgg aatctgttcg gcagcgactg gatcgacctg ccacagccgc
    28081 tgatgcagtc gggaccagcc gtcggcctcg gcctcgtcca gcagacgcgc gcgcagcgcc
    28141 ggccgcgccg gcggcagcgt ggtgatgccg cggcacagca actgaaagta gagcatggtg
    28201 ccgccgacca gcagcggcgt gttgccgcgg gcgcgaatct gcgccatcac cgccagcgcg
    28261 tcatcacgaa aggccgccgc cgagtaggga tcggccggat cgcgcaggtc gatcagatgg
    28321 tgcggatagc gcgcctgcag cgccggggtc ggcttggccg aaccgatgtc gagaccgcgg
    28381 tagacctgcg ccgagtcgac actgacgatc tccacggcaa agcggtccgc cagctccagc
    28441 gccagcgcgc tcttgcccga ggcggtgggg ccgagcagca gcagcgccag cggtgtgtca
    28501 tcgggcagcg tcattccacc cgccccggtc agccaagaaa cagccggtag gccggatttt
    28561 ccgactcatc ccaccagggg taaccgatgg ccgccagctc ctggttcagc ctggcgcgct
    28621 cgcttcttgg cacctgaata ccgctgagaa cccggccata ggccgcgcca tggttgcgat
    28681 agtggaacag cgtgatgttc cagcgctcgc ccagccggct cagaaagttg agcagcgcgc
    28741 cgggccgctc cggaaactcg aagcggtaga gccgctcgtc ggccacatcg gtggcatggc
    28801 cgccgaccat gtggcggatg tgcagcttgg ccatctcgtt gtcggtcatg tcgaccaccg
    28861 gatagccatg gtggtgcagc ttctcgatca gggcggcgcg cccttcgccc ggcgcgccct
    28921 cgttgacctc gacgccgacg aagatgcgcg cctgctgcgg atcgttgtag cggtagttga
    28981 actcggtgat gttgcgccgc cccagcaggg tgcagaaggc gtggaaactg cccggccgct
    29041 ccggaatggt caccgccagc accgcttcgc gccgctcgcc gagttcggcc cgctcggcga
    29101 tgtggcgcag ccggtcgaag ttgacgttgg caccgctcag cagcgccatc agggtctgct
    29161 gttcgacctg gttgcgctcg acatactgct tgaggccggc cagcgccagc gccccggccg
    29221 gttcggcgat ggcacggatg tcgctgaaca gatccttgat cgccgcgcag atctcatcgg
    29281 tggtgacggt gatcacctcg tccacgctct tgcgcagcac gcggaaggtc tcctggccga
    29341 tctgtgccac cgccacccca tcggcaaaca gtccgacctg cggcagcacc acccgccgcc
    29401 gtttggccag tgccgctgcc agacaggccg actcctcggc ctccacggcg atcaggcgaa
    29461 tctccggccg caggtatttg atgtaggcgg caatgcccgc catcaggccg ccaccaccca
    29521 ccggcacgaa gagcgcatcg agcgcgccgg tatgctggcg cagaatctcc atgccgatgg
    29581 tgccttggcc ggcaatgacg tcggggtcat cgtaggggtg gatgtaggtg agttgctgct
    29641 cggccatcag ctgctgcgag cgaatgaagg cctcatcgaa actatcgccc tgcagcagca
    29701 ccttgccgcc cagcgcgcgc acggcagcca ccttgatcgc cggggtggtg cggggcatga
    29761 cgatggtggc cctgatcttc agccgctggg ccgccagcgc caccccctgg gcatggttgc
    29821 cggccgaggc gcagatgacg ccggccgcgc gcgcctcggg cgtcagcttg accatcttgt
    29881 tgtaggcgcc gcgcagcttg aaggagtgca ccggttgcag atcctcgcgc ttgagcagaa
    29941 tctggttgcg gtagcgccgc gacagcagcg gtgccggctg aatcggtgtc tcgatcgcca
    30001 cgtcatagac gttggcttcg agaatcctgc ggatgtaact ctccggcatg gtggcggccc
    30061 gctgtcgata ggtcggtcgg tggcagcatg caattgtaca cggcgcggcc gcagtgaggc
    30121 cggtcgatgg gcggcggagg ctttggcatc caccgatccg tggcgcacaa tggcggccaa
    30181 cctcgacatc ggaatgccgc gcatgagcca gattctctcc cgcccccacc cccgttttcg
    30241 tccacggcgc atgcgccgcg acgacttcag ccgccggctg atgcgagaaa cccgactgag
    30301 cagcgatgac ctgatctatc cactcttcgt ggtcgagggc aacaaccggc gtgaaccgat
    30361 cggatcgatg ccgggcatcg agcgggtcag catcgatcaa ctgctgcacg aggcagaggc
    30421 agcgctgcaa ctcggcattc cggccattgc gctcttcccg gtcaccccgg cgcaactcaa
    30481 gagcagcgat ggcgccgaag cctgcaatcc gcaggggctg gcgcagcggg cgattcgcgc
    30541 ggtcaaggcg cgctttccag aactgggcgt gattgccgat gtggcgctcg acccttatac
    30601 cagccatggc caggatggca tcatcgatgc cagcggtcat gtgctcaatg atgtcaccat
    30661 cgaggtgctg gtccggcagg cgctgtcgca ggccgaggcg ggcgccgaca tcgtcgcccc
    30721 ctccgacatg atggatggcc gcatcggcgc gatccggcag gcgctggaag gtgccggcca
    30781 cagccacacc cgcatcctcg cctactcggc caaatatgcc tcggcctact acggtccgtt
    30841 ccgcgacgcg gtcggctcgg ccggcaacct cggcaagagc gacaagaaga gctaccagat
    30901 ggatccggcc aacctggagg aggcactgca ggaggtggca ctcgacctcg aggagggcgc
    30961 cgacatggtg atggtgaagc cgggcatgcc ctacctcgac gtggtgcagc gcatcaagcg
    31021 gcagttcggc gtaccgacgc tggtctatca ggtgagcggc gagtatgcga tgcaccgcgc
    31081 cgccatcgcc aatggctggc tgagcgaggc ggtgattatg gagtcgctgc tgtcgatcaa
    31141 gcgcgccggt gccgacgcga tcctgaccta cttcgccaaa gaggtggcca ccgagctgcg
    31201 ccggttcggc ggttgatctg gttgaaaaga ctcaccgaac cttcataatg aaacccgcct
    31261 tccgtgacca tccaacccac ttttttccgg agcagagcca atcatgagcg acgccatcgt
    31321 ccatgtgtcc gacgagactt tcgaacaaga ggtgctgaag agcccgacac cggtgctggt
    31381 cgactactgg gcagcctggt gtggcccctg ccggatgatc gccccggtgc tggatgagct
    31441 ggccgatgac tacgatggcc gggtcaaggt gtgcaaggtc gatatcgacc agaacgagat
    31501 gacgcccgcc aaatatggcg tccgtggcat cccgacactg atgctgttca agaatggtca
    31561 ggtcgaggca acccgggtcg gcgcgatgtc gaagtccgaa ctgaccacct tcatcgacag
    31621 caacctctga acctctgccg tccgccatcg cgggcctctt ttcgtccctt cccgtttccg
    31681 ccatggaggg cggacacccc gttcgaccac ccgtcagcac cgtctcaacc cggacctcgg
    31741 cacgccctgt gccattttga ccatgcggtc atccaattca tccactctgc ggtatgatcg
    31801 gcctgcccac atccgggcag cgtccgcgag gttccccgca tgaccccggc tcatccgcac
    31861 gacgacatcg gcctgctctt ctccctgctg ccactgccat tgcagcatgc cctctccctc
    31921 ctgccacgcg aacaactgct ggagatcgtg ctcgacctgg gccgggtgcc gcaggcgcga
    31981 ctgcgcgatg gtgcggtgat gctcggcagc gagccgatca gccatgacca gttgcagcag
    32041 atcgtcggcc ggatcagccc cttcggccag gacaaccgtg ccggcatcga gggcacgctg
    32101 caccggctgt cggccctgcg caaccgccgc ggtgagatcg tcggcctgac gctgcgggtg
    32161 ggacgggcgg tgatcggcac catcggcccg ctgcgcgacc tggtcgaacg cggcgccagc
    32221 ctgttgctgc tgggtcgccc cggcgtcggc aagaccaccc ggctgcgcga agtggcccgc
    32281 tttctcgccg atgagctggg tcggcgggtg atggtggtcg acacctccaa cgagatcggc
    32341 ggcgacggcg acattccgca tccggccatc ggctgcgccc ggcgcatcca ggtgccgacc
    32401 cccgagaagc agcatgcggt gatggtcgag gcggtcgaga accacatgcc ggaggtgatc
    32461 atcatcgacg agatcggcac cgccgccgat gtgctggcgg cacgcacgat cgccgagcgc
    32521 ggtgtgcagc tgatcggcac cgcccatggc aacacgctgg ccaacctgct ggccaatccg
    32581 accctctgcg acctgatcgg cggggtgcag acggtgacgc tgggcgatga gatggcacgg
    32641 ctgcgcggca cgcagaagac cattctcgag cgcaaggggc ccgccacctt cgactgcgtg
    32701 gtggagctga tcagccagga tgaactgctg atccatgccg acagcaacgg ctcggtcgat
    32761 gcactgctgc gcggcgagtc accgccggtg ctgcattgcg gcgacgcggc ggccgaacca
    32821 gcggatggcg aatctggcgt cgttcagtcg ctgttgccgc ggcgacgccg gctgacgccg
    32881 agcagtcaga ccccgccgct ggtcagcagc ggccatccgt tgatgatcta cccctacggc
    32941 gtcgaccgcg acctcatcga gcgggtgctg cgcgatctgc ggctgcatgc ccgcaccgtg
    33001 cggcgggtcg agcgtgccaa cctgattctg gcgctgcgcg agcaggtgga ggatcagcgg
    33061 ctgttgcgga tggtgcaggc gaccggcatc ccggtctact ttctgaagcg aaacagcacc
    33121 gcgcagattc gccacctgtt gcagcacaac ctcgaactgc agggcgtcgc cgccaacggg
    33181 acgcactgag aaaccgccgg ctcaaccctc ttcatgccgc actgcccgca gcgcgccgat
    33241 ggcctcgggc agcgcatact gctcgccgct gacaccggcg atgaaatggc cgccgcgaat
    33301 ctcaccactg gcctcgagcc ggcgatagat gcgcagcagg tcacgccagg gtggcagcag
    33361 ccggctttcg cgctccagca gttgccggtg gatgatgccg tagcggcgca gcaactgtcc
    33421 ggcgatggcg cgggtcacct gctcactgtg gcgggcgccg aaccagctgg cagcctgctc
    33481 ctgctgcaac ggtggcggca gcagcgacca gcggcccgcc tcggccagtg ccgagctgcc
    33541 acggcggcgg tagcgcgggt ggttcttcca ggccgccggc tgaatcagcc cgcgcatgcc
    33601 ggcaaagctg tcggcgctca cctcgccccg ctgcaccagt tcggcaagat aacgctccag
    33661 atggctcggc agcagactga gccgctggca cagctcgtcg aagaagagcg ccccgccact
    33721 cgccagcagc gcgcgcaccc gttcggcccc ggcgctgcac ggggtgcctt ggtcggcatc
    33781 ggccagcgcc cgccacagcg gaaactcgcc acgcggcagc agcatgatcg gcgtgcggcg
    33841 cagcggacca ccgcccttgc cgctgccgcc cagccgggtc cagaccaccc ggccgctcag
    33901 cgtgagctga tcgagccagg cggggtcgta gtgcgccagc cgggccggca gcaactcacc
    33961 ctcccactgg ctggcggtgg cttcgagccc ggccagttgc ccgatcacgg cattgagccg
    34021 ctcgacgccg ctgccgctgg cacccacccc ctgccagtgg tcgatgaagc ggtggaactg
    34081 cgcgatcgac accggttcga tggcgcgccg cagacggtcg agggtgtagc gattgatgcg
    34141 cgccagcagc cgccgctcgc accactgctc acgctgcggg tcgctgaagg cgccgcgcag
    34201 gatgaacccc tcctgctcca gcttcagcag cgccgccatg atgcgcggcg gcggcagtgc
    34261 aaacaggctg gccagttcgt cggcatcgag cggaccgctg ctctccagcc gggcgcgcag
    34321 cagctcgacc agcgcggcct cggccgtcca atcgaactct ttcaacggat cgggcagcgg
    34381 tggcagcggc tgctcggcca gttgcggatg cagtgccagc agttgtggca gccgctcgat
    34441 cgccagccag cgccgctctc cggccagttc gatgcacagg acccgcccct gcgcctgcgc
    34501 tgccgtcagc agcgtggacc agccggccgt gcgcggctgc gccagctcct cttcatgcag
    34561 caggccgacc agcagcagcg catcgcagag cgcatcacca tcgtgcgcca ccggccaggc
    34621 ttcgccgcgc acccgttcga tcgctgccgc atcgagccgt cccagatcgg cggcggtggc
    34681 cgggtcgagc cagcgccggc tgatcaccgc ctgggtgcgg cgctcctcga gcggggcatc
    34741 gtcgagaaag gcgtagggcc gggccgccag aatctcctgg gcgaagggcg aggcttcggt
    34801 cagctcggcg cagaccagcc tgatctcacc ggcgaccagc cgctgcagca gccgctccag
    34861 cccgtcgatg tccatcgcct cggtcagaca gtcgtcgagg gtctgcgcca ccagtggatg
    34921 gtcgggaatc tcccgctccc cttgcagatt ctcgaaacag gccaactggt cgggaaagac
    34981 cagcgcaatc agatcctcgg cttggatccg ctgcagaaag gccggcgtgc gctgaccgcc
    35041 acggcgacgg ggaatcgcca gcgcggtcag cgcattccag cgccagcgcg cctggaacag
    35101 cggtgcatcg agcagcgcct gcaccagcag cggccgcaca gtgcgcgcct ggaggtagcc
    35161 ggccacctcc gccaatggaa agctgtgggt cacgccgagc gagaggatga tgctgttctc
    35221 gctcgccgcg gcctgcagct cgaagttgaa ctggcggcag aagcgcttgc gcagcgccag
    35281 cccccaggcg cggttgatcc ggccgccaaa gggggcatgc agcaccagtt gcatcccgcc
    35341 cgattcgtcg aaaaagcgct ccagcaccag acagtcgtcg ctcggcatcg cgccgagcgc
    35401 ggcccgcgcc gccgccagat actccaccag ttgccagcag gcggccgccg gcagccccag
    35461 acgcgcctgc aacgccctgg ccgcggtctc gacactgccc tgttgcagcg attcatcgac
    35521 ctcgcgccgc aaccgcgaca ccgcccgcga cagcgcatcg ctgcgcgcag gcgcctcgcc
    35581 gagccagaag gggatggtgg gtggttggcc agccgcatcc tcgacccgca gccggcccga
    35641 ctcgaccttc acaatgcgcc agctgctgtt gccgagctgg aagatgtcgc cggccatgct
    35701 ctcgacggcg aaatcctcgt tgacgctgcc gatcagggtg ccggccggct ccaggatgac
    35761 gtcgtagtcg gcggtgtccg gaatggtgcc gccacaggtg agggcggtca gccgggcacc
    35821 cggtcgcggc cgcaacatcc cctgcagcag gtcacgatag aggcaggcgc cacgccgtcc
    35881 gcgccggctg ctgtagcctt caccgagcat ccgcaccagc tccgcaaagg tgtcgtacgg
    35941 caacgcgcgg tagggttggg cgcggctcag cagcgcatac agcgcccgct ccgaccaggg
    36001 ctcggccgcc acctcggcca cgatctgctg cgccagcaca tcgagtggtg cctgcggaat
    36061 gcgcaactga tcgagttcgc cccgtttgac cgcatcgatc agcgccgtca tctccaccag
    36121 atcgtcgcgg ctcaacggca gcagtcgccc cttcggcagg ccgccgacgc tgtggccgga
    36181 gcgaccgacg cgctgcaaca gcgtggcgat ggcgcgggtc gcgcccagtt ggcagaccag
    36241 atcgaccgcg ccgatgtcga tgccgagttc gagcgaggcg gtggccacca gcgcccgcag
    36301 ctcgcccgcc ttcagccgct gctcggcctc gaaccgctgc gccttcgaca ggctgccatg
    36361 gtgcgcagca accgcctcgc tgcccagccg ctcgctgagc tgccgcgtca cccgttcggc
    36421 cagccggcgg gtgttgacga agatcagcgt ggtgcggtgt tcgagaatca gcgcggcgag
    36481 ctggtcataa agcaggcccc agacctcgtt gctcatcacc gtttcgagcg gtgccgccgg
    36541 cagcagcagg tcgaggtcga gcgcccgtcg atggccctga tcgatgatgt ggcacggggg
    36601 cggcgccaca ccgtcgcccg cgccgaccag aaaatcggcc accgccgcga tcgggttctg
    36661 ggtggcggag agtccgatcc gggtcagcgg ctggcgcgcc agatgttgca gccgctcgat
    36721 cgacagtgcc agatgggcgc cgcgcttgct gtcgatcagc gcatggatct cgtcgatgat
    36781 cagcgtgtgg gtctggccga acagtgcccg tccgccgctg ctggtcagca gcaggtagag
    36841 cgactccggg gtggtgacca gaatgtgcgg cggccgcttc agcagcgcgg tgcgctccgc
    36901 cggcgtgctg tcaccgctgc gcagaccgac ccgcagcggc accgccggca gccccagcac
    36961 ctccagttca ctggcgatac cggccagcgg ccaggccaga ttgcgctgga tgtcgttgga
    37021 gagcgccttc agcggcgaga catagaccac tgcggtggcc tcgaccagtt caccagtcag
    37081 cccctgccgc aacagccggt cgatcaccgc cagaaaggcc gccagcgtct tgccggaacc
    37141 ggtcggcgcc gcgatcagcg tgtgctcacc gcgctggatc gccggccagg cggcgcgctg
    37201 cgccggcgtc ggcccgccaa aggtccgctc gaaccagcgc gccactgccg gatgaaacgg
    37261 cagcgcctcg gccgtctcct caagccgatc gggctgctgc atggtgaagg tttcgccggg
    37321 tggcgcgaaa tgaaaatcgc cgacgccgag gcatgtcggt gcgcagcacc gttgcctgcg
    37381 gcggcctaca gcgacgttgc gccatcggcc gtcgcccgag cgctcgtctc gtcgcggccc
    37441 acctggctgt ggttggcata ggtttcccag gtcggaaagt aatcgtccgc ctgctggccc
    37501 caggtggtcc aacccgtgcg gctgccgcgt gcgaacatct cgagccaggg gccaggagag
    37561 caggcttcga tgatgtcata ggcctcgtcc ggcttgcggc tgtgctcgcg cttcatcgtg
    37621 gccagaaagt tgacctgtcg ccgtcccggc gcgagcgtcc gggtaccctt gccccgcacg
    37681 ccgaacagaa tcagctcggt cacgttgcgg aaatagaagc cgaccccacg cccgtccggc
    37741 ccgcattgag acggggaagc aatttgccga tccaggtggt cgatgcgcca tatttggtct
    37801 tgacgccaag ggcggcaaat acggaatcgc gcgtctcgtg cgcgtcagca acacgccggc
    37861 cctcatggta gataccttgc cagatccaat gcgccgtgaa agacacccaa aatgtcgatc
    37921 agttgcgatg gcgatggccg ccgcagatag acgattcgat aatggccata cagcaggatt
    37981 ctgatctctc cttcgggttc gactcgatat cggtggccaa tttcgggaaa gtgccgcagc
    38041 acttgtgccc gctcaatgat gcccgccatg actttcgatg ccgccaccgg gttatccact
    38101 gcaatgtagt catggatgtc acgaagccaa cgtccggctt cctcggtcca gactatttct
    38161 gccatgtgcg aatccgttgc tccagctcat tctgggtcat cactctgccc tgacgggcgt
    38221 cttcgagacc ccgttccacc attccctcga acagcagttc gcgcatgatc tcttcatagg
    38281 tggcatcctc cggctgcgat tggatgacct ccgccatttt ctctttcacg ttcgacatga
    38341 gatctcccta cgcatcaatt ccgcagtggt gctgctggcg gatgcaaaac acgacgcccg
    38401 cgtggcgcac ctgtcaagcc agcccctacg gtaacggctt gcacgctcag cggcaatcca
    38461 gttcaactca cgccgctgtg taggtttggc cggccccgac cgctatactg ccggcctgcc
    38521 gttgcgaccg gaaccgatga tgccacgacg actcctgctg ctctgtgtga ccctctgctg
    38581 cctggccggc tgtggccagc gcgggccgct ctatctgccg ccgccgactg gcgatcaatc
    38641 cgcgccggca gccagcacgc cgccatcccc gctgcggaat ccatcatgat cgaccccttc
    38701 ggttaccgtc agggccggct gcatgccgag gatgtgccgc tcgagcagat tgccgccgag
    38761 ttcggcaccc cctgctatgt ctactcccgc gccgccatcg agcagaacta tctggccttc
    38821 gagcagccgc tgcagggcca gccccatctg ctctgctatg cggtcaaggc caactccaat
    38881 ctggcggtgc tcaacctgct ggcgcggctg ggcgccggct tcgacatcgt ctcggtcggc
    38941 gaactggagc gggtgctggc ggccggcggc gatccggccc gggtgatctt ctccggcgtc
    39001 ggcaagcgcg ccgacgagat ggcacgggcg ctcgatgtgg gggtgcgctg cttcaatgtc
    39061 gagtcgattc cggagctcga ccgcctcgaa gcggtcgccc gggcacatgg ccagcgcgcg
    39121 ccgatctcga tccgggtcaa tcccgatgtc gatgcccgca cccatgccta catcgccacc
    39181 ggccgcaagg agaacaagtt cggcatcgaa ttcgccgatg cgctggcgct ctacgggcgc
    39241 gcggccgcat cgccccatct cgaggtggtc ggcgtcgatt gccacatcgg ctcacagctc
    39301 accgagatcg agccctttct ggtcgcgctc gaacgaatgc tcgagctgat cgaccagctg
    39361 cgtgcccaag gcatcgagat caggcacctc gacctgggcg gcggactcgg cgtgcgctac
    39421 cgcgacgaga cgccaccgac accggcccaa tacatcaccg cgctcacccg gcggctggcc
    39481 ggaaccgggc tcgaactggt gctggagccg ggccgcgcca tcgtcgccaa cgccggcctg
    39541 ctgctgagcc gggtcgaata tctcaagcaa agcagcgtca agcgctttgc gatcatcgac
    39601 gcggcgatga atgatttgct gcgacccact ctatacaatg cctggcaggc agtctgcgag
    39661 gtgacccgcc gtggcggcga gccggtggag tacgaagtgg tcggcccggt ctgcgaaagc
    39721 ggtgattttc tcgccaaggc gcgtctgctc gacatcgagg cgggcgacct gctggcgatc
    39781 cgctcggcag gcgcctatgg cttcgtcatg gcctccaatt acaacagccg gggccggcct
    39841 gccgaggtga tggtgagtgg cacccagatg catctggtcc gcccgcgcga gaccctggcc
    39901 agcctctacg ccgacgagcg gctgctgccc tgagcgggcg cttttcccga cacaggaaca
    39961 gacactgatc aagggattcc gatgcggttg cgattcacca agatgcaggg tgccggcaac
    40021 gacttcatgg tggtcgacct ggtcacccaa caggcggtgc tgacaccgac ccagatccgg
    40081 caactggccg accgccactt tggcgtcggt ttcgatcaac tgctgctggt cgaacccccc
    40141 accgaccccg gcatcgactt caagtaccgc atcttcaatg ccgatggcgg cgaggtggag
    40201 cagtgcggca acggcgcccg ctgttttgcc cgcttcgtgc gcgaccagcg gctgtcgtgg
    40261 aaggagcgga tcaccgtcga gacgcgcggc ggcctgctcg aactgctgct ggagcgcgac
    40321 ggttcggtca ccgtcgacat gggtgcgccc gatttcagcc ccaaggcgct gccgttccag
    40381 gctgaggccg aagcgctgca ctatccgctc gaggtggccg gccagaccct cgagatcggc
    40441 gcggtatcga tgggcaaccc ccatgccgtg ctggtggtcg acgatgtcga acgggcgccc
    40501 gtgaccacgc tgggtccggc catcggcagc cacccgcgct ttcccaaggg ggtcaatgtc
    40561 ggcttcatgc agatcgtcga tcgcaccacc atcaagctgc gggtccatga gcgcggtgcc
    40621 ggcgagacgc tggcctgcgg gaccggcgcc tgtgccgctg cggtgatcgg catgcgccgg
    40681 gggcagctcg atgagcaggt cacggtcaga ctgaccggtg gcgagctgtt gatccgctgg
    40741 gccggcaatg gcgcgcatgt ctggatgagc gggccggcaa cacgggttta cgaaggttgg
    40801 attcagctat gacaatttcc ggacaggccg gtggcaacag ccacggtgag accagtgccc
    40861 ccagcgaggc agaggtggcc gcctacctcc atctgcaccg cgacttcttc ggtcgccacg
    40921 aaccgctgct ggccgagatg tactttccct ctccccaagc tggcgccatc tcgctggtcg
    40981 agcgccagct cggcatcctg cgcgaacggc tgcagagcac gcgtcacgag ctggcactgc
    41041 tgatgggccg cgccgaacag aatgaccggc tgatcacgct gacccagacc ctcagcctgc
    41101 aactgctcga cgccgcgcat ggcagcgaaa tcaatctgct gctgcgccgt gcgctgatcg
    41161 gcgagctggg caactgcgcg ctcagcctgc tggtggcgac accggggacg gcggccgccg
    41221 cgctcggctt ccacactccg gccagcgcgg aggccgagcg gcgcgtcgag gcggtgctgg
    41281 ccaaccgctg cgccagtctt ggcctgttcc gtgacgagga gctggcggca ctcttcggca
    41341 gcgagggcag cggcgtcggt tctgcggtgc tgctgccgat ccagcgcggt ccggtccagg
    41401 ccgtgctggc gctgggccat cccgaccgtg accactaccg cgccggcatg ggcaccctgt
    41461 tcatcagcca cctgggcgag gtcatcggcc gggtgatcga gcggctgctg aagagcgccg
    41521 cctgaagcgg gccgccccga tggacgcgca gatcgaccac ttcctgcgcc aactgcagca
    41581 gcagcgtgcg ctgtcgaacc acacccttgc ggcctaccgc catgagctga cgcgcttcgc
    41641 ccgctttgcc ggcgagcggc agctccagct tgccgacatc gatcagccgc agatccggct
    41701 gttcgccagc gaactgcggc gccacggcct cggcggccgg tcgatccagc gcgccctttc
    41761 cgcactgcgc agcttctttc gctttctgct gcgcgaaggg gtggtcggcg acaacccggt
    41821 gctcggcgtg cgcctgccgg gcagccggac ccggctgccg cgtccgttgc cggtcgagga
    41881 ggcggtgcaa ctggtcgaac tgaagggcga ggcgtggctg atccggcgcg accgcgcgct
    41941 gcttgaactc tgctacggcg ccggcctgcg gctggccgaa ctgaccagcc tgaactgcgc
    42001 cgacctcgat ctgaatgaac ggctggtgac cgtcaccggc aagggacgca agcagcggct
    42061 ggtgcccttt ggccgcgccg ccgcaacggc attgcgccag tggctgaccg tgcggggagc
    42121 gcggttggcc gacgaccgcg agccggcgct cttcatcagc cggcgcggcc agcgcatcca
    42181 tccgcgcacc gtccaggcga tgatgcacag gcgcggcgtc gagcaggggc tgaccagtcg
    42241 gctccacccc caccggctgc gccactcctt cgccacccac ctgctggagt cgagcggcga
    42301 cctgcgggcg gtgcaggagc tgctcggcca ccgcgacatc agcaccaccc agatctacac
    42361 ccacctcgat ttccagcatc tggccgccag ctacgatgcc gcccaccccc gcgcccgacg
    42421 gcgcggcaag agtgtgccat gacgccgccc cggctgaagc tggtcacctt cgacctcgac
    42481 gacaccctgt gggagagcgc gccggtgctg atcgaggccg aagcggccat gcagcgcgct
    42541 ctgcaacagc aactgcccgc gctcggcgac acattgcgct cgggcatcct gcgccagcag
    42601 cgcgcgctgc tgctggcggc ggagccggcg ctgatccatc gggtcagcga gctgcggcgg
    42661 cgggtcatcg caacgacgct gcgcgatctg ggcctcgacg ccgagcgcgc acggcagatt
    42721 accgaagccg ccttcgagca gttcctgcag gcgcgccacc aggtgcgtta ccacgccgat
    42781 gcgctggaga tgctcgaact gctggcgcgg cgttaccgcc tcggcgccct caccaatggc
    42841 aacgccgaca tccgccgcct ggggctcgac cgctacttcg acttcggctt cagtgccgag
    42901 atgctggggg tcggcaagcc cgacccggcg ctgttccggg ccgcgctcga cgccatccga
    42961 tgcagtccgg cgcagatggt g
//
LOCUS       DAORCQ010000006        41563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_465_length_41563_cov_5.456490, whole genome shotgun sequence.
ACCESSION   DAORCQ010000006 DAORCQ010000000
VERSION     DAORCQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 41563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41563
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_465_length_41563_cov_5.456490"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKI23_01055"
     CDS             <1..532
                     /locus_tag="PKI23_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNN35557.1"
                     /translation="ITPDYLCLSKGLTGGYLPLSVVLTRESIYRAFYDDYNTLKAFLH
                     SHSFTGNPLACRAALATLELFAIEPVLERNRTLAAAMARSTAHLVDHPNVAEVRQHGM
                     VLAIELMRDKARREPYPWQERRGIRVYRHALEQGVLLRPLGNVIYFMPPYVISEEEIE
                     LLATVATSGIELAVRD"
     gene            539..1288
                     /locus_tag="PKI23_01060"
     CDS             539..1288
                     /locus_tag="PKI23_01060"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNN35558.1"
                     /translation="MATARIHVDLPLVVGATLTLPEAASHHLLRVLRLERGAALTLFN
                     GEGGEFAARLLGVNGRLAQVELVAQRATSTESPLFSHYGLAISKGERMEIALQKLTEL
                     GVSRITPLLTARCEVRLGAERWERKRQHWQQVVISACEQSGRTRIPAIAPPCALETFA
                     AQAEGDLKLFMHWQGRALPSGQQPRRLVLVSGPEGGLTEAEADCLERAGFLRVTVGPR
                     ILRTETAPLTLLSLGQWLWGDLDGGAASPTE"
     gene            complement(1337..2080)
                     /locus_tag="PKI23_01065"
     CDS             complement(1337..2080)
                     /locus_tag="PKI23_01065"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNN35559.1"
                     /translation="MLLAYGRFLFKYRNALFPVLFLSLALFFPPTAAFGSAMADRLLD
                     LAGFAVLLAGLAMRALVIGLEYIRRGGKDGKVYAVDLVTGGIFASCRNPLYVGNLLIL
                     AGMVLIWHNWVVYAVATLFGISSYIAIVAAEEDFLLDKFGDAYRAYCADVNRWLPNPD
                     KLAEALANGRLNLAQIVVKDYNTWFTWFSTVLLLLFYERRSAAEYLAEAGSTGWIWGT
                     MALLFVLYVLVYFLKKSQRLNARGWNSNR"
     gene            complement(2115..2864)
                     /gene="cmoA"
                     /locus_tag="PKI23_01070"
     CDS             complement(2115..2864)
                     /gene="cmoA"
                     /locus_tag="PKI23_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HNN35560.1"
                     /translation="MSTSDSNAPPRDQVFAQPLDRVTDFAFDSTVVAVFDDMVSRSVP
                     FYDEMQRMTCEIARDFAVPGSSLYDLGCSTATTMLQLDPVIDPSVRFVGVDNSPEMLA
                     QARSKLQQHGVTRPFDLVCADLHHDTVVEDASVVAMLLCLQFMRPLYRERVMRGIYDG
                     MRRDGALILIEKLTVDDSLINRLFIQHYYDYKRRHGYSELEISQKREALENVLIPYRW
                     EENYNLLKWAGFRHVEVFFRWYNFCGILAVK"
     gene            3003..4361
                     /gene="gorA"
                     /locus_tag="PKI23_01075"
     CDS             3003..4361
                     /gene="gorA"
                     /locus_tag="PKI23_01075"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNN35561.1"
                     /translation="MSVHDYDLLVIGAGSGGVRAARTAAGLGARTAVVEAGPLGGTCV
                     NVGCIPKKLLVYAAEFSHTWEEAAGYGWTLPEAPRFDWPQLIQNKNREITRLNGVYQR
                     LLEGAGVTVIAGTARLTGPNEVRVGERHYSAAHILIATGGMAELPEIPGRELAITSDE
                     AFYLPQLPDSVAVVGGGYIAVEFAAIFNGLGARTHLIHRSGQLLRNFDHDLGAALCEE
                     MAHDGIELHLHQQIERIDAGVAGRKRLLLQDGEVIEVDQVLFATGRRPNSAGLGLEEL
                     GVECTANGAIQVDAHYRTRVPSILAIGDVLGGWQLTPVALAEAMAVARTIVLNQPSRA
                     DYDAIPTAIFSQPQIATVGLSEQEARRRGPIRLFRSRFTPLRHTLSGRKSRTLVKLVV
                     DAQSDRVLGAHMVGEGAAEILQGFAVAVKAGLTKRQFDATLGIHPSAAEEFVTLRQEV
                     EA"
     gene            4399..5163
                     /locus_tag="PKI23_01080"
     CDS             4399..5163
                     /locus_tag="PKI23_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNN35562.1"
                     /translation="MAMSKLCDLQLDGPIATLSLNRPETLNRLPAAELPHLLELLDRV
                     EADARLRVLVLTAAGNKTFCAGFDISDIGSEPQSAVTLDDVVSRLAALSIPTVCALNG
                     AVYGGGVDLSLGCDFRIGVEGMQLQIPAARLGVHYYPNGMRRLIELLGLGMARRLLLL
                     AQRLTAEELLACGYLDQLVPLADLPLTTAQMAQRLTEHAPLAVRGMKSCLNQLARGLF
                     DEARARQGMEAAMNSQDLREGARAFQEKRSPRFKGR"
     gene            complement(5451..6722)
                     /locus_tag="PKI23_01085"
     CDS             complement(5451..6722)
                     /locus_tag="PKI23_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNN35563.1"
                     /translation="MFDTAALDAVLQLSVALAIGLLVGLERGWKSRELGEGRRIAGFR
                     TFGLTGLLGGVAGLLSLDQGPLPIGLIFLALALLLAVVYARTTSADPAANISITTLIA
                     LLLTFALGALATYLDIALAAAAAVVATLLLDLKSTLHGWLNRLEERELTALLRLLLIS
                     VVVLPFLPNEQLGPWQAINPHELWWMVVLISGISFYGYVMVKLLGQRRGLALTALFAG
                     LTSSTALTLHFSRLARQQPEQARLLGSGILFSCSAMLPRMLLMILVLNAALIPPLLLP
                     FMAMEAVILLPALHGWLRADRVGTFEPTPLSNPVELKAAFGFGLLLTLVVILCAAARQ
                     LIGDEALLPVALISGIADVDAITMLLSRLSLTEVSPTTSLAGIIAVAVSNNLAKAALA
                     ILIGGQRTGWRVALPLIGSAGVGLSFWLLWL"
     gene            complement(6796..7851)
                     /locus_tag="PKI23_01090"
     CDS             complement(6796..7851)
                     /locus_tag="PKI23_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNN35564.1"
                     /translation="MTYLAKPKLHHPDLPRNQLGLTHRDYEGAISTLCAGCGHDSISA
                     AIVQACYEMSLPAHRIAKLSGIGCSSKTPTYFLGLSHGFNSVHGRMPSVLTGAYMANK
                     DLIYIGVSGDGDSASIGLGQFAHSMRRGINMTYIVENNGVYGLTKGQFSATADYGAVS
                     KRGVKNFDEAIDLVTLAIQMGATFVARSFSGDKRQLVPLLKAAIHHQGAAFIDVISPC
                     VTFNNHKGSTKSFDYVREHNAVVNRPDFIPLRDEITVDYPAGAVEAVTLHDGSVLRLA
                     KCDHDYNPRDRVAAINCIQERQQRGEVVTGLLFIDPDAVDLHEEMGNVDTPFNQLDDA
                     ALCPGNAALQKLNQSFR"
     gene            complement(7854..9683)
                     /locus_tag="PKI23_01095"
     CDS             complement(7854..9683)
                     /locus_tag="PKI23_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNN35565.1"
                     /translation="MSQPLKSINDFVVKFANVNGTGSASANLLFAKSILRMGVPIAPR
                     NIFPSNIQGLPTWYEVRVSERGHLGCRGGVDFMVAMNPQTWDQDIASIEPGGYLLYDS
                     TKPLPASKFRSDITVFGVPLMEICNREPGDARQHQLLKNVLYVGVLSALLDIELEVLD
                     QLIAEEFKGKAKLIAPNQKTLRLGRQYALDHFACPIGLRVERADGVGERIFIDGNTAA
                     ALGALYGGATVAAWYPITPSSSVAEAFMHHAERMRVDAEGKRKYAVIQAEDELASIGI
                     VIGAGWNGARAFTATSGPGISLMTEFLGLAYFAEIPAVLFDVQRGGPSTGMPTRTQQS
                     DILACAYASHGDTKHILLLPDGPGECFEFGALAFDLADRFQTPVFVMSDLDIGMNDRL
                     CAPLSWDDSRRFDRGKVMDFAALEAGKTFGRYLDVDGDGVCYRTYPGTHPARGAYFTR
                     GTTRDPFARYSEEGDVYVDNMERLLRKFETAKSWVPAPLELDAGREYGVIFYGSSAPA
                     MDEAVELLAARGLQLDTLRVRAFPFAASVREFVERHRLVFVVEQNRDAQLRTLLINEE
                     EIDPARLLPVCHYDGTPITARAIIAGIEARLDLLKVTPLRKRS"
     gene            complement(9668..11464)
                     /locus_tag="PKI23_01100"
     CDS             complement(9668..11464)
                     /locus_tag="PKI23_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNN35566.1"
                     /translation="MEPTNILDPDYFHRVVDCQWACPAHTPVPEYIRLIAAGRYDDAY
                     LVNWHSNVFPGILGRTCDRPCEPACRRGRVEQEPVAICRLKRVAADYKSDIRARLPQA
                     PFASNGKRIALIGAGPASLTVARDLLPLGYQVDLFDGAPSAGGMIRTQIPAFRLPESV
                     IDEEVGYVLDLGVKAHFGHWVGSLVELLTKGYDAIFIGTGAPRGRDLDLPGRQQAAAS
                     IHLGIDWLSSVSFGHTQKIGARVIVLGGGNTAMDCCRTARRLGGEDVRVVVRSGFDEM
                     KASAWEIEDAMHEDIPIHNFLVPKAFLHDNGRLTGMLFEPVQPVYDEAGQRKLLPTGE
                     PLVEMACDEVLIAVGQENSFPWIERDIGIEFNRWGLPVLDETTLQSTLPKVFFGGDAG
                     FGPKNIIWAVAHGHQAALSIDLFCRGESPAARPPAQIALASQKLGIHEWSYDNEISLD
                     QRRKVPLQEKRIALLNLNLEVERGFDPDLAYKEAERCLNCDMQTVFTASQCIECDACV
                     DICPVDCITFLPQMSEASEAQLREQLKVPAHQLDQPLYLSTALNTGQVMIKDENVCLH
                     CGLCAERCPTAAWDMQKFVIDLPKAGASCRSH"
     gene            complement(11693..12439)
                     /locus_tag="PKI23_01105"
     CDS             complement(11693..12439)
                     /locus_tag="PKI23_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35567.1"
                     /translation="MRNLFLLLLLLNLLLALWEGQPHLLRQHGRVALLTPAAVDELPS
                     WVLRAELDAAAGTPEPPTEPMPTLPLARLQPVTPEQGATEREPLRCYQLSPFVDAAQR
                     DGVLRRLASANFVLLAPLPTLSFDVSWALTLPLPVGATKARLERSLSALGFEHHVGQD
                     QAGVAQQIVSGFASVEAANAAQLRLAQQGFLFRVERRQAPPSVQRITLATASIEAVAE
                     RLEALLQNGDGHRLEAVVVPCEGELVPVEP"
     gene            complement(12464..13198)
                     /locus_tag="PKI23_01110"
     CDS             complement(12464..13198)
                     /locus_tag="PKI23_01110"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNN35568.1"
                     /translation="MLLEFDLGNSRAKWRLLGAPPGELASGDFRIGPEGVIGTLALPA
                     QQLPISRIRAGSVAGAQINEALTQLCRQWWNLVPEFAQSQAHCAGVSNGYQFPQRLGV
                     DRWLALLAAYHRPDRQNRPCAVVDCGSAITLDLLQADGKHLGGYIVPGLRMVSEVLSS
                     RTRLIRIDELPLGEISPGRVTDQAIGHGVMRMLHGLLDNLEEYLGRDYRLYFTGGDGE
                     RLLRSLKLPGSYHSDLVLDGLAWAVP"
     gene            complement(13198..14187)
                     /gene="birA"
                     /locus_tag="PKI23_01115"
     CDS             complement(13198..14187)
                     /gene="birA"
                     /locus_tag="PKI23_01115"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:NF008847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HNN35569.1"
                     /translation="MSDAELQNRQLLTHLADGQFHSGEVLGRLLGISRAAIWKRLQRL
                     QESAGIAIERRHGKGYRIDGGLDLLDAAAIRAQLDPEALRQPLRLDLHWSTDSTNSRL
                     VEAAQTASIHGLVCLAERQTAGRGRRGRNWFSPFGGNLYLSLGWSFNAGVSTLEGLSL
                     AVGVALARTLEQLGIEGALLKWPNDLLIDQGKVAGILIETVGDMDSHCQVVIGVGVNV
                     RMPAAAEAAIDQRWSDLQRHLAEPLSRNRLTALLLNQLVAMLSQFHRQGFAAFAEAWN
                     QRDLCAGQAVVIRGLHSAIEGIACGVDQRGGLQLRTAAGIEVIRSGEVSLRMQ"
     gene            14297..14599
                     /locus_tag="PKI23_01120"
     CDS             14297..14599
                     /locus_tag="PKI23_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35570.1"
                     /translation="MVKIHGAVEGKSQSRSTHASMITTNETGPDVGRCLFTLFESGQA
                     GVQRIDIWNGNATQTLWCSPVADGHASFDASCWTPFSVTAPLCHQAAQWLQLEWRL"
     gene            complement(14537..17314)
                     /locus_tag="PKI23_01125"
     CDS             complement(14537..17314)
                     /locus_tag="PKI23_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNN35571.1"
                     /translation="MEPRQHVLMCRERSTWPCWLMAALLWLLGSAMPLSAQPVVLDEA
                     SSCTDITAHLQIYTDPSNQVDIRTLLTPAYQHQFMPLKRSRLWLNRPDATTWIKFEIA
                     PGDPHQPNRLLHLGHASLSEATLFAVDGSGKIINPRSVDPTFDDSDSRAALKQVFLIK
                     PVSQSTTYYLRLHHPLPIALDPTIWVPNALLQKIEWFQWMSGTFFGLMTAVILFNLLM
                     FFANREPANLYYGAFLLSMTLLQALIDGKFFNLVPIDAKWLGKSAILAVLLTNYTALR
                     FARVFLLQDAHEGRWSRLNRLGEWIVILAALSALFLSNIVLLYQLALACSALFTLFLL
                     LLSLYRAQQGYRPALYYFCGRMLLLPVLLMMILHALGGTDIQILAGKGVMGVAAIEGI
                     ILSLALLHRIRLMEQWHLAERHQDERRQVEIRARRKLIAKITQELRDPLSGIHGMSEL
                     LIDADLGKDEKNQARTIHQAAEKAIYLVDRAQNTVGLESDTLQIHVDEFDLQQLLDEV
                     VAQTRLIADPQRITLTVALDPRLAETAQGDPRRIKQALVELVSNAIAAIHAGEVRIGV
                     EALSESPGLRFTVSDQGPGIPEPIQQLLDLPLHRMLVELESREQGGLGLPIVKWLVTG
                     MGGQLRFVSHAGRGSSISFTLPSPVSLPVSSSTAADGIRLSGQRILIVEGNETIRDTL
                     LIRCRRWGMDALAASNSNQAIALIRDQGHLGKGFDFLLLEQESTPLSGLELALKINQE
                     GLSPSPVMILLLGFGAAPTSERLRHAGIAKVLTKPIHGAQLQRLLGDLLPRKAIESTS
                     HSPDADSRVLIFERDPATLDHLRKLFLKLKVSVEGVSERSSLLQRIKQTHYDLILIHH
                     EPPLLDALELGQRIRAWELQSNRLPTPLVVIDGPDRPESDWQASGINARLEPPFELEP
                     LRRLMAQWCGD"
     gene            complement(17343..18935)
                     /locus_tag="PKI23_01130"
     CDS             complement(17343..18935)
                     /locus_tag="PKI23_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN35572.1"
                     /translation="MSMTHSAVRSPASSHYQGRNVPWLLAHWAAVRPDHPFLIWEPFS
                     GATRSWSYRQFHAEVRRLAAGLAALGVKPTERVLIHLDNCPESLLAWYACAELGAVGV
                     TTNTRSAGPEVAYFAAHSQAVGAITQPAHADLIAREAKGIRWLAVTDNDAGAPPLSSE
                     FLPDQAIPFGSLLADSMAPAYPSAALAPVGIQYTSGTTSRPKAVVWTHANALWGAQIN
                     ARHLELTSNDVTLTHLPLFHTNAQSYSVLGSLWVGGTVVLQPRFSASRFWEVSLRHGC
                     TWSSMIPFCLKALLQQERPPRHSYRVWGNGICAPPTDAYFGVTSLGWWGMTETITHGI
                     VGDLHFPNASLSCGRPAPEYEIAVLDDDGVPVDPGETGHLLIRGIRGVSLFLEYLDNP
                     EATRDSFDEAGWFRTGDRVRPMPDGHVFFADRDKDMLKVGGENVAASEIESVIRGTGW
                     IDEVAVVAQRHPMLDEVPAAFVIANPDAPADLAARLIDHCRTQLADFKVPRNVMVVDE
                     LPRSTLEKINKAELRKRLPVSE"
     gene            complement(19012..20034)
                     /locus_tag="PKI23_01135"
     CDS             complement(19012..20034)
                     /locus_tag="PKI23_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNN35573.1"
                     /translation="MTRYHVYGIGNALVDKEFEVEEHFLTEQGIDKGVMTLVPHARQE
                     QLLTQLKQRYGVKKRASGGSAANTIIAVSHFGGKCYYACKLGDDEPGDFYLHDLTQAG
                     VSSNINGARPAGGVTGRCLVMVSADAERTMHSFLGISATLDIEDIDFDALKRSEFLYL
                     EGYLVTSDSSRAAAIAAREFAERHGIKTSLTFSDPNMVQHFKPGISAMLGNGVDLLFC
                     NEHEARLFTGAESLMDAAEQLRRVAKQFAITCGARGALLYDGAGLIEVDSVPVHAIDT
                     NGAGDMFAGAFLYALNHGYDFTAAGRLANRAAAAVVSRFGPRLEAEQHAELLRQHAEK
                     SWNQNR"
     gene            20172..22457
                     /gene="mrcB"
                     /locus_tag="PKI23_01140"
     CDS             20172..22457
                     /gene="mrcB"
                     /locus_tag="PKI23_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HNN35574.1"
                     /translation="MQLRSGLIKSLVALAALLTAFCVYLDAQIVDYFEQHRYQPPARV
                     HARPLLLREGELLSEERLTTELAALGYRFVAGLKEPGEAHREAGEVAFWHRGFRFAEG
                     DEPARRLRLRFAGGRITELRQEDGRDVASVRLEPLEIGSIETRLHEDRQPLAIDQVPQ
                     PLIAILLAVEDRRFFDHHGISLWSILRASWANLRAGQVEEGGSTLTQQLVKNLFLSGE
                     RSLWRKGVEALMALLLELHYDKSLILETYLNEIYLGQQGPRAIHGVELASRHYFSRPL
                     DQLNLPEMALLVGMIKGPSLYDPFRDPVAARGRRDVVIERLLAERMITPEQATASRNA
                     PLGVRAREFANQHPAYLDLVRRELQHDQVDLKNNINGLRIFTHLDPEIQRQAERALRE
                     GIDALERKHALPAGTLNGAVVMAEIDSGQVVAVVGDRQGTFFGFNRALDSRRPIGSLI
                     KPAVYLTALTQPSRYRLDTPLEDQPLRLRDANGRSWSPGNYDQKSHGSVPLYRALANS
                     YNQATVRLGLDLGLPQVIRTLHQLGVEQPIAAYPSLLLGTLERSPLELLQIYQTIAAG
                     GRHVPLHGVGGLTAQQGVPPALPPVSAEQRFDAAEVALLHHGLYAVVTEGTARRLDEQ
                     LGIARRVIGKTGTTNDQRDSWFAGFDGRYVAVVWLGRDDNQPMPLTGATGALEVTARL
                     YGAIDSRPWRWSAPAGVQMAAIQTAAGCRELPFVSGSEPVQSGCGVGSRLGATAGEAA
                     QSSQSTVKGTLKSWFNRLLGR"
     gene            22662..23390
                     /locus_tag="PKI23_01145"
     CDS             22662..23390
                     /locus_tag="PKI23_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003210307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNN35575.1"
                     /translation="MLDRTRIVLLRTSHPGNIGAAARAMKTMGLTRLLLVDPVRFPAP
                     EAEFRSAGGADVLAQAQVVATLDDAIDSCQLVVGSSARSRRVPWPLLDPRQLAERLSQ
                     LPAQTEIAILFGNEQHGLSNEELHRCHWHVQIPADAEYGVLNLAAAVQVISYELRMQW
                     LQAGDIATVSNSWDVPLATSSEIEQLFLHLQKLLLELDFYDPDNPRQLLTRLRRLLLR
                     SGLDRMEVNILRGILAAAMARIDH"
     gene            23412..24209
                     /gene="cysE"
                     /locus_tag="PKI23_01150"
     CDS             23412..24209
                     /gene="cysE"
                     /locus_tag="PKI23_01150"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNN35576.1"
                     /translation="MLTRLREEIDSIFERDPAARTLWEVLTCYPGLHAVLLHRVSHWL
                     WQHRLKWLGRFLSTLTRWLTGIEIHPGARIGRRFFIDHGMGVVIGETAEIGDDCTVYH
                     GVTLGGTSWQPGKRHPTLRDGVVVGAGAKILGPIEVGVGARIGSNAVVTKNVPSEATV
                     VGVPGRIIQPLTGEPPSTSSPHREAIARKFGFDAYGLGRDVPDPVARSIHAMLDHIHA
                     LDTKLDGICGALKALGSDHCAETIEILDEVIDLEGGVPPGASGRANT"
     gene            24273..24764
                     /gene="iscR"
                     /locus_tag="PKI23_01155"
     CDS             24273..24764
                     /gene="iscR"
                     /locus_tag="PKI23_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNN35577.1"
                     /translation="MRLTTKGRYAVTAMLDLAIHAIEGPVSLADISERQEISLSYLEQ
                     IFARLRQNKLVSSVRGPGGGYRLSRDRQEISVVQIIDAVNESIDSTNCGGQGNCQRGE
                     QCLTHQLWCDLSEQIHLFLAGITLAHLIEKREVQDIAKRQRDFIARERGTESLAHIRL
                     GTS"
     gene            24807..26021
                     /locus_tag="PKI23_01160"
     CDS             24807..26021
                     /locus_tag="PKI23_01160"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNN35578.1"
                     /translation="MRVPIYMDYAATTPVDPRVAQKMSECLLMDGCFGNPASRSHQFG
                     WKAEEIVETARRQVADLINADPREIVWTSGATESDNLAIKGVAHLYGEQGRHIITSKI
                     EHKAVLDTCRQLEREGFEVTYLDPDSTGLITAEQVGQALRDDTILVSIMHVNNEIGTI
                     NDVAAIGEVTRARKVLLHVDAAQSAGKVEIDLERMKIDLISFSAHKIYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGMRSGTLATHQIAGMGEAFRIAKEEMGVECSRIAALRDR
                     LWQGISSLDAVHLNGHAERHVCGILNVSFACVEGESLLMSLKDLAVSSGSACTSASLE
                     PSYVLRALGLSDELAHSSIRFSIGRFTTEQEIDHAIEQVRKAVTRLRELSPLWEMHQS
                     GVDLSTVQWAAH"
     gene            26078..26461
                     /gene="iscU"
                     /locus_tag="PKI23_01165"
     CDS             26078..26461
                     /gene="iscU"
                     /locus_tag="PKI23_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNN35579.1"
                     /translation="MAYSEKVLDHYENPRNVGSLDVNDVNVGTGMVGAPACGDVMRLQ
                     IKVNEAGIIEDAKFKTYGCGSAIASSSLLTEWVKGKSLNEAAQIRNQEIADELALPPV
                     KIHCSVLAEDAIKAAIADIKQKRGE"
     gene            26492..26815
                     /gene="iscA"
                     /locus_tag="PKI23_01170"
     CDS             26492..26815
                     /gene="iscA"
                     /locus_tag="PKI23_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNN35580.1"
                     /translation="MGIQLTEQAAEHIRQQLDARGHGIGVRIGVTPSGCSGMSYLLEF
                     VDQLNEEDQVFEKDGAKVIIDAKSLPYLEGTQLDFVREGLNEGLKFNNPNVKAECGCG
                     ESFTV"
     gene            26852..27367
                     /gene="hscB"
                     /locus_tag="PKI23_01175"
     CDS             26852..27367
                     /gene="hscB"
                     /locus_tag="PKI23_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNN35581.1"
                     /translation="MRSYFQLFDLPQCYQLDLERLDRNYRNLQASVHPDRFVGDGAQA
                     RRLAEQYSGLLNQAYTTLKSPVERAAHLLQLLGVEIDGAATISDGDFLFRQMTLRETL
                     HDVRQQRDETALLQLRQQALAEQNGLQQAFSRAIKDPQNLQSALQFLLKMRFFQKFLA
                     DIHDVEDVLLD"
     gene            27414..29282
                     /gene="hscA"
                     /locus_tag="PKI23_01180"
     CDS             27414..29282
                     /gene="hscA"
                     /locus_tag="PKI23_01180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNN35582.1"
                     /translation="MALLKIAEPGQSTPAARPRRRAVGIDLGTTYSLVATVEAGQVKV
                     LSDSDGGVLLPSVVHYRAEGLPEVGVAAQSLAIVDPLNTIASVKRLMGQGMAAAQRLS
                     DHPLEASANGMPYLRTAAGNKSPVEVSAEILRALAERAERHFNETGVAAVITVPAYFD
                     DAQRQATKDAARLAGIELLRLLNEPTAAAIAYGLDQNAEGVIAVYDLGGGTFDISILR
                     LSRGVFEVLATGGDSQLGGDDFDRRLAQWLLSETGHPEPLTPAESRHLLGVARAAKER
                     LSEQVQTTVELNEGNLCWQGVLTRSQFEQLIGDEVERTLRACRRALRDSGCELKQIDE
                     VVMVGGSSRVPVVRERVEALFGRTPRIDIDPERVVAMGAARQADLLAGNAQQQALLLL
                     DVIPLSLGIETMGGLVERIIPRNSPIPVSRAQEFTTHQDGQTAMSIHVVQGERDLVEE
                     CRSLARFVLRGIPPMSAGAARIRVTYQVDADGLLSVSATELTSGVASSIEVKPSYGLG
                     EEEILRMLRDSRAFAAEDMRARALREQRIAADALLQSLDHALTQDGDKLLAATERQQL
                     LQQMARLRTLMAGDDPQQIALAVEQLNRESQSFAERRMDLILRSALAGKSIEEVKG"
     gene            29284..29622
                     /gene="fdx"
                     /locus_tag="PKI23_01185"
     CDS             29284..29622
                     /gene="fdx"
                     /locus_tag="PKI23_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019384153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNN35583.1"
                     /translation="MPTIKVLPHPQRCPQGALFEAKPGDNLIELLLAHDIEIEHACER
                     SCACTTCHVIIRQGFDSLDEAADLEEDMLDKAWGLTLTSRLSCQVTVGEQDLLVEIPK
                     YTINLASENH"
     gene            29633..29824
                     /gene="iscX"
                     /locus_tag="PKI23_01190"
     CDS             29633..29824
                     /gene="iscX"
                     /locus_tag="PKI23_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNN35584.1"
                     /translation="MKWTDVQQIAIALTERHTDVDPQYVRFTDLHRWVCELEGFADDP
                     LRSNEKILEAIQAAWLDEL"
     gene            29950..30381
                     /gene="ndk"
                     /locus_tag="PKI23_01195"
     CDS             29950..30381
                     /gene="ndk"
                     /locus_tag="PKI23_01195"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNN35585.1"
                     /translation="MAVERTLSIIKPDAVGKSVIGKIYSRFEKAGLRIVAAKMVHLSN
                     REAEGFYAEHRERPFFKDLVGFMTSGPVMVQVLEGENAVLLNRELMGATDPKKADANT
                     IRADFAQSIDENAVHGSDSAQSAAREISYFFAATELCPRTR"
     gene            30405..31538
                     /gene="rlmN"
                     /locus_tag="PKI23_01200"
     CDS             30405..31538
                     /gene="rlmN"
                     /locus_tag="PKI23_01200"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNN35586.1"
                     /translation="MGETTRINLLGLSRPKLEQFFAELGEKRFRVDQTLKWLHHRRAD
                     SFDQMSDLSKGLRTRLGEVAEIRPPEVVHRQRSLDGTRKWLIRVESGSCVEMVFIPQN
                     GRGTLCVSSQVGCALDCSFCSTGKQGFNSNLTAAEIIGQVRIAVDELAGLYPERDRAV
                     TNVVLMGMGEPLLNFDNVVDAVQLMMDDFGYGISKRRVTLSTAGLAPMIERLGEVTDV
                     SLAISLHATTNALRDQLVPVNRKYPIEVLFAACQRYFARLGEHRSLTIEYTLLDGIND
                     QPEQARELAVLSRILPSKINLIPFNPFPNSGYRCSSPAAIDAFLQILQREGCRATIRA
                     TRGDDIDAACGQLVGQVSDRTRRQQRHAQRHAAELLAQPLTLR"
     gene            31545..32342
                     /gene="pilW"
                     /locus_tag="PKI23_01205"
     CDS             31545..32342
                     /gene="pilW"
                     /locus_tag="PKI23_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNN35587.1"
                     /translation="MSAPVVSCCRRLLVTLLLALLAGCVTQETTPFNKRDQAKAVEAY
                     IALSIAYLRDGNAQMAKESLQKAKALDPDNPALFSATAYLFQWQGEPQLAEENYRKAL
                     SRNPQFSEARNNYGVFLYSQGRYDEAISELLKVAEDPLYAQRHQAYENLGYCALKKND
                     SALAIHYFSKALKINENQPNALLEMADLMLQAGNLSAANAHMGRFRDLLKMRQAEASA
                     RSYWIELQLARAQGNHDLEASNALKLKGLFPDSEEYRSYLKQSGRVP"
     gene            32391..33503
                     /gene="ispG"
                     /locus_tag="PKI23_01210"
                     /gene_synonym="gcpE"
     CDS             32391..33503
                     /gene="ispG"
                     /locus_tag="PKI23_01210"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNN35588.1"
                     /translation="MQPQQSIVRRKSRKIWVGSVPVGGDAPIAVQSMTNSDTHDVAAT
                     VAQIQALADAGADIVRVSVPTMEAAEAFRQIRAQVVLPLVADIHFDHKIALRVLEHGV
                     DCLRINPGNIGKEERIRAVVDAARDRRVPIRIGVNAGSLERDLQRKYGEPTADALVES
                     AMRHVEILDRLDFADFKVSVKASNVMMAVAAYRKIASQIEQPLHLGITEAGGLRSGTV
                     KSALGIGTLLMEGIGDTLRVSLAADPVEEVKVGFDILRSLQLRSRGINFIACPSCSRQ
                     NFDVIATMNALEQRLEDVRVAMDVAVIGCYVNGPGEAREVEIGVTGAAPQNLIFIDGK
                     PSHKVAAEALTDELERLIRERAAQKSRQAANTIARG"
     gene            33536..34819
                     /gene="hisS"
                     /locus_tag="PKI23_01215"
     CDS             33536..34819
                     /gene="hisS"
                     /locus_tag="PKI23_01215"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNN35589.1"
                     /translation="MKINAIRGMNDILPEETPLWRYAEKIIQQVLTTYGYREIRLPLL
                     EPTLLFDRSIGEVTDIVEKEMYTFPDRNGESLTLRPEGTAGCVRACQEHGLLFNQTQR
                     LWYQGPMFRYERPQKGRYRQFHQIGVEAFGFAAPELDAELLLLSAELWRQLGLTELVT
                     LEINSIGSAEARGRYRADLVAYLSTRKEKLDDDSRRRLDSNPLRILDSKVPETQALLD
                     QAPVITDYLDQPSRDHFARLRALLDAMQIPYRINPRLVRGLDYYCDTVFEWVTSALGA
                     QGTVCAGGRYDGLVEQLGGRPTPAVGMAMGLERLILLLGSRTRQPGELGPHSDLYLIA
                     ASDAELPAVMQLAERLRRALPQRVIITHLEGGSFKSRMKRADKSGAEFALLLGEDELR
                     DQVVSLKPLRSEAPQQRFDEATLIDHLRQHFRVTL"
     gene            34959..35672
                     /locus_tag="PKI23_01220"
     CDS             34959..35672
                     /locus_tag="PKI23_01220"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN35590.1"
                     /translation="MDPYLSEQEQIEAIKSWWRRHGSTLLITLALLLAAYWGWQLWQK
                     QQADSQARAAALYQNMEQAYLLAQKGEKEEQAQQLATFTHLAGQLKSDYPTYGYARFA
                     AWMLAKAAVDRQDYQAAEKELEWVLAHLGMGWMKGEDTLSAAISRLRLAQLKLAQKQF
                     DAALAQLDLVSDGAFEVREAELRGDVLMAKGDAAGAVAAYTRALAATAVDDPLRGLTE
                     MKLDDAKSRLPKTNTEQKP"
     gene            35669..36820
                     /gene="bamB"
                     /locus_tag="PKI23_01225"
     CDS             35669..36820
                     /gene="bamB"
                     /locus_tag="PKI23_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNN35591.1"
                     /translation="MNNLARALPLLLLCLLAACSSDSKKKINLPTPLVDVGNEVALDE
                     KWSRSIGKINVTSYNRIQLALDQGTLYAATIDGTVIAVDATTGERRWEVETKARISGG
                     VAAGRDRVLVTTLDGELIALSSNDGSELWRSAIKSELLSVPAITAQAIVVQGVDDRLH
                     GLEPTSGREIWSHTVLTPVLTLRGTASPVGNDQSVIAGFANGKLMAFDAGSGVSQWEA
                     VVAQAKGRSELERMVDVDGTPLLLEHLLFAASHQGRIAAFSAADGKPLWAQEASSFQP
                     LAGDAERIYLVSNDDEVQAFALESGKPLWKQPALQYRKLSPPTHWQQWLVVADSEGYL
                     HLLAASDGHIAGRYRASRDAIGAMMIADGEQLYLLDDGGKLRALGLKAR"
     gene            36817..38214
                     /gene="der"
                     /locus_tag="PKI23_01230"
     CDS             36817..38214
                     /gene="der"
                     /locus_tag="PKI23_01230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNN35592.1"
                     /translation="MIPVIALVGRPNVGKSTLFNRLTHSRDALVANLPGLTRDRRYGE
                     ALLQGQRTILIDTGGIDGERSGVDAAAEAQSRQAIKEADLVLFLVDCHAGLTAADEAV
                     GAELRRLGKKTLLVANKIDGANPDLILSEFHAMGLGDPQPIAAAHGRGLQSLIEAVLE
                     RLPPQPEEPETPPAETGVKIAVLGRPNVGKSTLVNRMLGEERVVVFDQPGTTRDSIYI
                     PYERHGRRYTLIDTAGIRRRKNIDEAVEKFSIVKALQAIEDAHVVLLLVDARENLVDQ
                     DLHLLGTALEAGRAVVIVVNKWDGLDPEQRARIRSELERRLAFIDFARIHFISALHGT
                     GVGELYKSIDEAYRAATRKLQTHKLTLLLQAAVVQHPPPVGGSGRRPKLRYAHAGGQN
                     PPLIVIHGNQVDSIPESYRRYLERFFRARLHLSGTPIKVEFRGGENPYAERKNTLTER
                     QLRKRRRLLEHVKKR"
     gene            complement(38217..39080)
                     /locus_tag="PKI23_01235"
     CDS             complement(38217..39080)
                     /locus_tag="PKI23_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNN35593.1"
                     /translation="MKPPAPTWAIVTGCLWIGLLLGNASPLAALPRAAAIPGGVVLLP
                     LPGHEAATQPPVARYQGRRVMVVSAALTGHPTSARWLAVVGIPLDAKVGPQRLEIEPG
                     RQIDFTIGPAHYPEQHITVTNQRQVTPLPDDLLRIERERQQIDAAFDHWSSPTLPVFT
                     LAAPVEAPRSGTFGVRRFFNGQPRQPHSGLDLAAPRGTAIQAPAAGRVIEIGDYFFNG
                     KTVLIDHGHGLVTLYCHLEQVDVQRGASVQAGDTLGRVGMSGRATAPHLHWGVSLNGV
                     RVDPALFLPPP"
     gene            complement(39077..40459)
                     /gene="xseA"
                     /locus_tag="PKI23_01240"
     CDS             complement(39077..40459)
                     /gene="xseA"
                     /locus_tag="PKI23_01240"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNN35594.1"
                     /translation="MSSVTPPSRPTPPILSVSQLNSLARELLEEAFALVWVEGEISNL
                     SRPASGHLYFTLKDERAQVRCALFRTRGRSLGFNPAEGDRVVARARLSLYEARGDYQL
                     IIEGLEPAGDGALRRRLELLKQKLLAEGLFDSARKRPLPAFVQRIAVITSPSGAAIHD
                     ILTVLKRRFAAIEVTLLPVAVQGAAAAGEIIAAIATANRLADQFDLILLARGGGSLED
                     LWCFNDEQLVRAIFHSRLPVVSAVGHETDFTLADLVADLRAPTPSAAAELISPSQEAL
                     LATLTHHLERLQGAMRQRLQRHQADLTMLERRLRHPGQRLREQAQRVDELELRLRHGL
                     LRQLRQSESRLQLLNGRLWQQLPNRRLDQHGSQLEALQRRLRQALRQRLDQRQHRLEQ
                     LLATLNAVSPLQTLARGYAIASRAGDGLLLRDADQVEPGQPVAVRLHRGRLLCRVEQR
                     ESEPDEVQDA"
     gene            complement(40514..>41563)
                     /locus_tag="PKI23_01245"
     CDS             complement(40514..>41563)
                     /locus_tag="PKI23_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN35595.1"
                     /translation="PKGIKLELKDEPIDFANSLVQLAVGLFGFNKDTVYLSPAPLYHA
                     APLRWCMTTHRVGGTVIVMEKFDPENALQLIEKYKATASQWVPTHFVRMLKLPEEVRT
                     KYDLSSLKCAVHAAAPCPVPVKEQMIQWWGPVIYEYYAGSEGNGGTAITSTEWLAHKG
                     SVGKSYGMPIHICDDEGNELPTGQSGTIYFEGGATFEYHNDPKKTAESRHPSNTDWST
                     LGDVGYLDEEGYLFLTDRKAFMIISGGVNIYPQETENLLVTHPKVADCAVFGVPNEEF
                     GEEVKAVVQLMNPQLASPELEKELIQFCRDNISHLKCPRSIDFTDELPRHPTGKLYKR
                     LLKDRYWGNRDSKIV"
BASE COUNT         7740 a        12774 c        13514 g         7535 t
ORIGIN      
        1 catcaccccc gattacctct gtctctccaa agggttgacc ggcggctacc tgccgctgtc
       61 ggtggtgctg acgcgcgagt cgatctaccg cgccttttat gacgactaca acacgctgaa
      121 ggcgtttctg cattcgcaca gcttcaccgg caatccgctc gcctgccgtg ccgcgctggc
      181 gaccctcgag ctcttcgcca tcgagccggt gctggagcgc aaccggacgc tggcggcggc
      241 gatggcgcgc agcacggccc acctggtcga tcacccgaat gtagccgaag tgcgccagca
      301 tggcatggtg ctggcgatcg aactgatgcg tgacaaggcg cgtcgtgaac cctatccatg
      361 gcaggagcgt cgcggcatcc gcgtctaccg ccatgcgctc gagcagggcg ttctgctgcg
      421 tccgctcggc aatgtgatct acttcatgcc gccctatgtg atcagtgaag aggagatcga
      481 gctgctggcc accgtggcca cttcaggcat cgaactggcg gtccgcgact gacccgcgat
      541 ggccactgcg cgcatccatg tcgatctgcc gctcgtcgtc ggcgcaaccc tgaccctgcc
      601 cgaagctgcc tcgcaccacc tgttgcgggt gctgcgcctc gaacgcggcg ccgcgctgac
      661 cctgttcaat ggtgaagggg gtgagttcgc ggcccgtctg ttgggcgtca acgggcgatt
      721 ggcgcaggtc gaattggttg cgcagcgcgc cacctcgacc gaatcgccgc tgttcagcca
      781 ctatggcctg gccatcagca agggcgagcg gatggagatt gcgctgcaga agctgacgga
      841 actgggggtg tcgcgcatca cgccgctgct gactgcacgc tgcgaagtgc ggctcggcgc
      901 ggagcgctgg gagcgcaagc ggcagcactg gcagcaggtg gtcatcagcg cctgcgagca
      961 gtcgggccgt acccgcattc ccgccatcgc ccccccctgt gcgctcgaga cctttgccgc
     1021 tcaggccgaa ggtgacctga agcttttcat gcactggcag ggcagggcgc tgccgagcgg
     1081 gcagcagccc cggcggttgg tgctggtcag tgggccggaa ggggggttga ccgaagccga
     1141 ggccgactgc ctcgaacggg ccgggttttt gcgggtgacg gtcggaccgc gcatcctgcg
     1201 caccgagacc gcgccactga cgctgctgag cctcggtcag tggttgtggg gtgatctcga
     1261 tggcggagcg gcttcaccga cggagtgatg gcacgccagt gccgccctgt gctcctgggg
     1321 tgacccgttt cagcatctag cggttgctgt tccagccacg ggcattgagt cgctggctct
     1381 tcttcagaaa gtagaccagc acataaagaa cgaacagcag cgccatcgtg ccccagatcc
     1441 agccggtcga accggcctcg gcgaggtact cggccgccga cctgcgttca tagaacagca
     1501 gcagcagcac ggtgctgaac caggtgaacc aggtgttgta atccttgacc acgatctggg
     1561 ccagattgag cctgccgttt gcgagggcct cggccagctt gtccggattc ggcagccagc
     1621 ggttcacgtc ggcgcagtag gcacggtagg catcgccgaa cttgtcgagc agaaaatcct
     1681 cctcggccgc gacgatggca atgtaactgc tgatgccgaa gagggtggcc accgcataga
     1741 ccacccagtt gtgccagatc agcaccatgc cggccaggat cagcagattg ccgacataga
     1801 gcggattgcg gcaactggcg aaaatgccgc cggtgaccag atccacggca tagaccttgc
     1861 catccttgcc gccacggcgg atgtattcca gaccgatcac cagcgcgcgc atcgccagtc
     1921 cggccagcag cacagcgaaa ccggccaggt cgagcaggcg gtcggccatc gcgctgccaa
     1981 aggcggcggt gggtggaaag aacagcgcca gagagagaaa cagcaccgga aacagcgcgt
     2041 tgcgatattt gaacagaaag cggccatagg cgagcagcat gggggagtct cgacgggatg
     2101 ggcggaagaa gcggctactt gacggcgagg atgccgcaga agttgtacca gcggaagaag
     2161 acctcgacat ggcgaaagcc ggcccatttg agcaggttgt aattctcttc ccagcgatag
     2221 gggatcagca cattctcgag cgcttcgcgc ttctgcgaga tctccagctc agagtagcca
     2281 tggcggcgct tgtagtcata gtagtgctgg atgaacagcc ggttgatcag gctgtcatcg
     2341 acggtcagtt tttcgatcag aatcagggcg ccgtcgcggc gcattccgtc ataaatgccg
     2401 cgcatcaccc gctcgcgata gagtggccgc atgaactgca agcagagcag catcgccacc
     2461 accgaggcat cctcgaccac ggtgtcgtgg tgcaggtcgg cgcagaccag atcgaatggc
     2521 cgtgtcaccc catgctgttg cagcttgctc cgtgcctggg cgagcatctc tggtgagttg
     2581 tcgacgccga cgaagcgcac gctcgggtcg atcaccggat cgagttgcag catggtggtg
     2641 gcagtggagc agccgaggtc gtagaggctg ctgcccggca cggcgaaatc gcgggcgatc
     2701 tcgcaggtca tccgctgcat ctcgtcatag aacggcaccg agcggctgac catgtcatcg
     2761 aacaccgcaa ccacggtcga gtcgaaagcg aaatcggtga cgcgatcgag cggctgggcg
     2821 aacacctggt cgcgcggggg ggcattggag tcggaagtgg acacggattc gatctcttga
     2881 tcttgtgaaa ggtggcaaag tctgttgttg taatcgggag ttcggcgcgt cagggcgatg
     2941 cggccggtac catggcaacg cgatcttatt cagttttctg tgaattctac aggagtcagc
     3001 ctttgtctgt tcatgattat gacctgctgg tgatcggggc gggttccggt ggcgtgcgtg
     3061 ccgcacgcac tgccgctgga ctgggcgcac gcacggcagt ggtcgaagcc ggcccgcttg
     3121 gcggcacctg cgtcaacgtc ggctgcatcc ccaaaaagct gctggtctac gctgccgagt
     3181 tcagccacac atgggaagag gccgccggct atggttggac gctgcccgaa gcgccacggt
     3241 tcgactggcc gcaactgatc cagaacaaga atcgtgaaat cacccggctg aatggcgttt
     3301 accagcgcct gctcgaaggg gccggcgtga cggtcattgc cggcactgcc cgactgacgg
     3361 gtcctaacga ggtgagggtg ggcgagcgcc actacagcgc tgcgcacatc ctcattgcca
     3421 ccggtggaat ggccgaactg ccggagattc cgggccgcga actggccatc acctctgacg
     3481 aggctttcta tctcccccaa ctgcccgaca gcgtggcggt ggtgggcggc ggctatatcg
     3541 ccgtcgaatt tgccgccatc ttcaatggcc tgggcgccag aacccatctg atccaccgca
     3601 gcggccaact gctgcgcaat ttcgaccacg atctgggggc tgccctgtgt gaggagatgg
     3661 cgcacgacgg catcgaactg catctgcacc aacagatcga gcgcatcgat gcgggggtgg
     3721 ctggccgcaa acgactgctg ctgcaggatg gagaggtgat cgaggtcgat caggtgctct
     3781 ttgccactgg gcggcgaccc aacagcgctg gcctggggct ggaggagttg ggcgtggagt
     3841 gcaccgccaa cggcgcgatt caggtcgatg cgcactatcg gacccgggtg ccgtcgatcc
     3901 ttgccattgg cgatgtgctg ggcggttggc aactgacgcc ggtggcactg gccgaggcga
     3961 tggcggtggc gcgcaccatc gtgctgaacc agccgagccg tgccgattat gacgccattc
     4021 cgaccgccat cttctcacag ccgcagatcg ccaccgtcgg actgagcgag caggaggcac
     4081 gccgccgtgg accgatccgt ctgttccgca gccgcttcac cccgctccgg cacaccctca
     4141 gcggccgtaa gagtcgcaca ttggtcaagc tggtggtcga tgcgcagagt gaccgcgttc
     4201 tgggtgcgca catggtcggc gagggggcgg ccgagatcct tcagggcttc gcggtggcgg
     4261 tcaaggccgg gctcaccaag cggcagttcg acgccaccct cggcatccat ccctcggccg
     4321 ccgaggagtt cgtcacgctg cgccaggagg tcgaagcgtg ataccgtgcg gccgttgttt
     4381 cactctcggg cgaagggcat ggcgatgagc aaactgtgcg atctgcaact ggatggtccc
     4441 atcgccaccc tcagcctcaa ccggcccgag accctcaacc ggctgccggc cgccgagctg
     4501 ccgcaccttc ttgaactgct cgatcgggtc gaggccgatg cccgcctgcg ggtgctggtg
     4561 ctgaccgccg ccggcaacaa gaccttctgt gccggtttcg atatcagcga catcggcagc
     4621 gaaccgcaga gtgctgtcac actcgacgac gtggtgagcc ggttggccgc gctgtcgatt
     4681 ccgacggttt gcgcgctcaa tggcgcggtc tatggcggtg gcgtcgacct cagtctgggc
     4741 tgcgactttc gcatcggcgt cgaggggatg cagttgcaga ttccggcggc ccggttgggg
     4801 gtgcactact accccaacgg catgcggcgg ttgatcgaac tgttgggtct cggcatggcg
     4861 cgccggctgt tgctgctggc gcagagactg acggccgagg agctgctggc ctgcggctat
     4921 ctcgatcaac tggtacctct ggccgatctg ccactcacca ccgcgcagat ggcgcagcgg
     4981 ttgaccgaac atgcgccgct ggcggtgagg gggatgaaat cctgcctcaa ccagctcgcc
     5041 cgcggcctgt tcgacgaagc acgcgcccgc cagggcatgg aagcggcgat gaactcgcag
     5101 gatctgcgcg aaggcgcccg cgcctttcag gagaaacgtt caccgcggtt caaggggcgc
     5161 tgaggcgcga tctttccgga gacgcagcag tatcgcaaca ccctgtgcat cgactgcgcc
     5221 cgtccatcgg cgggcatgtc gaggccgcag tgcttgattc aaccgtccag caaacccggc
     5281 atggttcagg ctcgaatcat ctccatcatc cccctccaat cgctctgatt tcccgtaaaa
     5341 cggaagcgga ttcggatgat tctccgatca gttctgcagg gaaggtacgt tttctgacag
     5401 gccgacacgt ccgtttcagc gcggcagcgc ctgccgcgat ggccggcagt tcagagccag
     5461 agcagccaga acgacaggcc gacgccggcg gagccgatca atggcagtgc cacccgccag
     5521 ccagttcgct ggccaccgat caggatcgcc agcgccgctt tggccaggtt gttgctgacc
     5581 gccacggcaa tgatgccggc cagcgaggtg gtcgggctca cttcggtcag gctgagccgg
     5641 ctgagcagca tggtgatggc gtcgacatcg gcaatgccgg agatcagcgc caccggcagc
     5701 agcgcttcgt cgccaatcaa ttggcgcgcg gcggcacaga ggatgaccac caaggtcagc
     5761 agcaggccga agccgaaggc cgccttcagt tcgaccgggt tgctgagcgg ggtcggttcg
     5821 aaggtgccga cacggtcggc ccgcaaccag ccatggaggg ccggcagcag gatgaccgcc
     5881 tccatggcca tgaacggcag cagcagtggc ggaatcaggg cggcattcag caccaggatc
     5941 atcagcagca tgcgcggcag catggccgaa caggagaaga gaatgccgct ccccagtaac
     6001 ctggcctgtt cgggctgctg gcgcgccagt cgcgaaaagt gcagcgtcag tgcggtggat
     6061 gaggtcagcc cggcaaagag cgcggtcagc gccagtccgc ggcgctggcc caacagcttg
     6121 accatcacgt agccgtagaa ggagatgccc gagatcagca ccaccatcca ccagagttca
     6181 tgggggttga tcgcctgcca gggaccgagc tgttcatttg gcagaaacgg cagcaccacc
     6241 accgagatca gcagcaggcg cagcagcgcg gtcagttcgc gctcctcgag ccgattgagc
     6301 cagccatgca gggtcgactt gaggtcgagc agcagcgtgg ccaccactgc ggccgcggca
     6361 gccagcgcga tgtcgaggta ggtggccagt gcccccagcg cgaaggtgag cagcagtgca
     6421 atcagggtgg tgatgctgat gttggcggca gggtcagccg aggtggtgcg ggcatagacc
     6481 actgccagca gcagcgccag cgccagaaag atcagcccga tcggcagcgg accctggtcg
     6541 agcgagagca gtccggccac gccgccgagc aggccggtca acccgaaggt gcgaaatccg
     6601 gcgatgcgcc gcccttcgcc cagctcccgg ctcttccagc cccgttcgag gccgaccaac
     6661 aggccgatcg ccagggcgac gctcagttgc agcactgcat cgagcgctgc agtgtcaaac
     6721 atcgtggatc caggctggcg cgcggccgcc ccctcctgat tgtggtctga ccggtggcgc
     6781 tatggcgtca tctcctcagc ggaagctctg attcagtttt tgcagcgccg cgttgccggg
     6841 acagagtgcg gcatcgtcga gctggttgaa gggggtgtcg acattgccca tctcttcatg
     6901 cagatccact gcatcgggat cgatgaagag cagaccggtc accacctcgc cgcgctgctg
     6961 ccgctcctgg atgcagttga tggcggccac ccggtcacgc gggttgtagt catggtcgca
     7021 cttggccagt cgcagcacgc tgccatcgtg cagcgtcacc gcttcgactg cgccggccgg
     7081 gtagtcgacg gtgatctcgt cacgcagcgg aatgaagtcg gggcggttga ccacggcatt
     7141 gtgctcacgc acgtaatcga agctcttggt cgagcccttg tggttgttga aggtgacgca
     7201 gggtgagatg acatcgatga aggccgcccc ttggtggtgg atggccgcct tgagcagcgg
     7261 caccagttgg cgcttgtcgc ccgagaagct gcgggcgacg aaggtggcgc ccatctggat
     7321 cgccagcgtg accagatcga tcgcttcgtc gaagttcttc acgccccgct tgctgaccgc
     7381 gccatagtcg gcggtggccg aaaactgccc cttggtcagc ccgtagacac cgttgttctc
     7441 gacgatgtag gtcatgttga tgccacggcg catgctgtgg gcgaactgcc caagaccgat
     7501 cgaggccgag tcgccatcgc cggagacgcc gatgtagatc agatccttgt tggccatgta
     7561 ggcgccggtc agcaccgagg gcattcggcc atgcacgctg ttgaagccgt gcgacaggcc
     7621 gagaaaatag gtcggggtct tcgatgagca gccgatgccg gagagcttgg cgatgcggtg
     7681 ggccggcaga ctcatttcgt agcaggcctg tacgatggcg gcgctgatcg aatcatggcc
     7741 gcagccggcg cagagggtcg agatcgcccc ctcgtagtcg cggtgggtga gcccgagctg
     7801 gttgcgtggc agatcgggat ggtgcagctt cggtttggcc agataggtca tgttcagctc
     7861 cgtttgcgca gtggggtcac cttgagcaga tcgagcctgg cctcgatccc ggcgatgatc
     7921 gcccgggcgg tgatcggcgt gccatcatag tggcacactg gcagcagccg cgccggatcg
     7981 atctcctctt cattgatcag cagggtgcgc agctgcgcgt cgcggttctg ttcgacgacg
     8041 aagaccaggc ggtgacgttc gacgaattcg cgcaccgacg cagcaaacgg gaaggcccga
     8101 acccgcagcg tgtcgagttg gagtccccgg gccgccagca gctcgacggc ctcatccatg
     8161 gcgggcgctg acgagccgta gaagatcacg ccatattccc gaccggcgtc cagctccagc
     8221 ggtgccggca cccagctctt ggcggtttcg aacttgcgca gcagccgctc catgttgtcg
     8281 acatagacat cgccctcttc gctgtaacgg gcgaaggggt cgcgggtggt gccgcgggtg
     8341 aagtaggcgc cacgggccgg atgggtgccg ggataggtgc ggtagcagac gccgtcgccg
     8401 tcgacgtcga ggtagcggcc gaaggtcttg cctgcctcca gcgcagcgaa atccatcacc
     8461 ttgccgcggt cgaacctgcg gctgtcatcc cagctcagcg gtgcgcagag gcggtcgttc
     8521 atgccgatgt cgaggtccga catcacgaag accggtgtct ggaagcggtc ggccagatcg
     8581 aaggccagcg caccgaactc gaaacattcg cctggcccat cgggcagcag caggatgtgc
     8641 ttggtgtcgc catggctggc gtaggcgcag gcgagaatgt ccgactgctg ggtgcgggtc
     8701 ggcatgccag tcgaggggcc gccgcgctgc acgtcgaaca gcaccgccgg aatctcggca
     8761 aaataggcca gcccgaggaa ttcggtcatc aacgagatgc cagggcccga ggtggcagtg
     8821 aaggcgcggg cgccgttcca gccggcgccg atgacgatgc cgatcgaggc cagctcatcc
     8881 tcggcctgaa tcaccgcgta tttgcgcttg ccctcggcat cgacgcgcat ccgctcggca
     8941 tggtgcatga atgcttccgc caccgaggag gaaggggtga tcgggtacca ggccgccacc
     9001 gtcgcaccgc cgtacagtgc accgagcgcc gccgccgtgt tgccatcgat gaagatgcgc
     9061 tcgccgaccc catcggcccg ctcgaccctg agtccgatcg gacaggcaaa gtgatcgagc
     9121 gcgtactgcc gccccagccg caaggtcttc tggttggggg cgatcagctt ggccttgccc
     9181 ttgaactcct cggcgatcag ttgatcgagc acctcgagtt cgatgtcgag cagtgccgac
     9241 agcacaccga catagagcac gttcttcagc aactggtgct ggcgggcatc gcccggctca
     9301 cggttgcaga tctccatcag cggcacgccg aacaccgtga tgtcactgcg gaacttgctg
     9361 gccggcagcg gcttggtcga atcatagagc aggtagccgc ccggctcgat cgaggcgatg
     9421 tcctgatccc aggtctgcgg attcatcgcc accatgaagt cgacgccgcc acggcagcca
     9481 agatggccac gctcgctcac ccgcacctca taccaggtcg ggagtccctg gatgttggag
     9541 ggaaagatgt tgcgcggcgc gatcggcacc cccatgcgca gaatcgactt ggcaaacagc
     9601 aggttggcac tggccgagcc ggtgccattg acgttggcga acttgacgac gaagtcgttg
     9661 atgcttttca gtggctgcga catgaggccc ccgccttggg cagatcgatg acgaatttct
     9721 gcatgtccca ggctgcggtg gggcagcgct cggcacacag accgcaatgc agacagacgt
     9781 tctcatcctt gatcatcacc tgaccggtgt tgagcgccgt ggagaggtag agcggctgat
     9841 cgagctggtg cgccggcacc ttcaactgct cgcgcaactg cgcctcgctg gcttcactca
     9901 tctgcggcag gaaggtgatg cagtcgaccg gacagatgtc gacacaggca tcacactcga
     9961 tgcactggct ggcggtgaag accgtctgca tgtcacagtt gaggcaacgc tctgcctcct
    10021 tgtaggccag atccggatca aagccgcgct ctacctccag attgaggttg agcagcgcga
    10081 tgcgcttctc ttgaagcggt accttgcgcc gctgatcgag cgagatttcg ttgtcgtagc
    10141 tccactcgtg aatgccgagc ttctggctgg ccagcgcgat ctgggccggt ggccgcgccg
    10201 caggcgattc gccacgacag aacagatcga tcgacagtgc cgcctgatgg ccatgggcca
    10261 ctgcccagat gatgttcttt ggtccaaaac cggcatcgcc accaaagaag accttgggca
    10321 gtgtcgattg cagtgtggtc tcgtcgagta ccggcagccc ccagcggttg aactcgatgc
    10381 cgatgtcgcg ctcgatccag ggaaagctgt tctcctgccc caccgcgatc agcacctcat
    10441 cgcaggccat ctcgaccagc ggttcaccgg tcggcagcag cttgcgctgc cccgcttcgt
    10501 cgtaaaccgg ctgtaccggt tcgaacagca tgccggtcag tcggccgttg tcatgcaaaa
    10561 acgccttggg caccagaaag ttgtggatcg ggatgtcttc atgcatcgca tcctcgatct
    10621 cccaggccga ggctttcatc tcgtcgaagc cgctgcgcac caccacccgg acatcctcgc
    10681 cgcccaaccg ccgtgcggtg cgacagcaat ccatcgcggt gttgccgcca cccagcacga
    10741 tcacccgcgc accgatcttc tgggtgtgac caaaggaaac gctggagagc cagtcgatgc
    10801 cgaggtgaat cgacgcggcc gcctgctggc gacccggcag atcgaggtca cgcccgcgcg
    10861 gggcaccggt gccgatgaag atggcgtcgt agcccttcgt caacagttcg accaggctgc
    10921 cgacccagtg gccgaagtgg gccttgaccc ccaggtcgag cacatagccg acctcttcgt
    10981 cgatcacact ctcgggcagg cggaatgccg gaatctgggt gcggatcatg ccgcccgcgc
    11041 ttggcgcgcc atcgaacaga tcgacctgat aacccagcgg cagcaggtcg cgcgccacgg
    11101 tcagcgaagc cggcccggcg ccgatcagcg cgatgcgctt gccattgctg gcaaagggcg
    11161 cttgcggcaa ccgtgcgcga atgtcgctct tgtagtcggc cgccacccgc ttcagacggc
    11221 agatggccac cggctcctgt tcgacccggc cgcgccggca ggccggttcg cagggacggt
    11281 cgcaggttcg cccgagaatg ccgggaaaga cattggagtg ccagttgacc agataggcat
    11341 cgtcataacg gccagcggca atcagacgga tatattcggg aaccggggtg tgggccggac
    11401 aggcccattg acagtcgacc acccgatgaa agtaatcggg atcaagaatg ttggtgggtt
    11461 ccaacgatgt cctcctgact gacgtgacgt aaagcggtgc aaacctgact gacgacgcac
    11521 tgctgttccg caagcgaagt cagaactccc tatcgacctc gaaaaaatcg cgcaatgata
    11581 ccgtaacgtt tcattcgagg gtaagccggt ggtcacattt tggtgcgggt ccggccaccc
    11641 gtggcgccag caatgatcag ccatgccgca gcggtgtgcc aacggcgctc gatcagggtt
    11701 cgaccggaac cagttcaccc tcgcacggca ccaccaccgc ttcgaggcgg tggccatcgc
    11761 cgttctgcag cagggcctca agccgctcag cgacggcctc gatcgaggcg gtggcaaggg
    11821 tgatgcgctg gacagagggc ggtgcctgcc gtcgctcgac ccgaaacaga aagccctgct
    11881 gcgccagccg cagttgcgcc gcattggcgg cctccacgct ggcgaaaccg ctgacgatct
    11941 gctgcgccac tcccgcctgg tcctggccga catggtgctc gaatccgagc gcgctcagac
    12001 tgcgttcgag tcgggccttg gtggcaccaa ccggcagcgg cagtgtcagc gcccaggaga
    12061 catcaaagct gagtgtcggc agcggcgcca gcagcacgaa attcgcgctg gccagccgtc
    12121 gcagcacgcc atcacgctgc gctgcgtcaa cgaaaggaga gagttggtaa cagcgcagcg
    12181 gttcgcgttc cgttgcgccc tgctccgggg tgactggttg caggcgggcg agcggaagag
    12241 tgggcatcgg ttcggttggc ggttcgggtg tcccggcggc ggcatcaagc tccgcgcgca
    12301 gcacccacga cggcagttcg tccactgctg ctggggtcag gagcgcgacc cgcccgtgct
    12361 gccgcagcag atggggttgc ccctcccaca acgccagcag cagattcagc agcaacaaca
    12421 gcaggaacag gtttctcatg gcgacatccc ggtctgaaaa gcgtcacgga acggcccagg
    12481 ccaggccatc gagcaccaga tcggagtggt agctgccggg cagcttcagg cttcgcagca
    12541 gccgctcgcc atcgccaccg gtgaagtaga gccggtaatc cctaccgaga tactcctcca
    12601 gattgtccag caggccatgc agcattcgca tcacaccatg cccgatcgcc tggtcggtga
    12661 cccgccccgg cgaaatttcg cccagcggca attcatcgat cctgatcaat cgggtgcggg
    12721 agctgagcac ctcactgacc atgcgaagac ccgggacgat gtaaccaccc agatgcttgc
    12781 catcggcctg cagcagatca agggtgatgg cgctgccaca gtcgaccact gcgcaggggc
    12841 ggttctgccg gtccggtcga tggtaagccg ccagcagggc gagccagcgg tcgacgccca
    12901 gccgctgggg aaactggtag ccattgctga ccccggcaca gtgcgcctga ctctgggcga
    12961 actcgggcac caggttccac cattgccgac agagctgggt cagcgcctca ttgatttgcg
    13021 ctccggccac gctgccggcg cgaatccggc tgatcggcaa ctgctgtgcg ggcagtgcca
    13081 gggtgccgat cacgccctcc ggaccgatgc gaaagtcgcc gctggccagc tcgcccggtg
    13141 gcgcgccgag cagacgccat ttggcccggg agttgccgag atcgaattcg agcaacatca
    13201 ctgcatcctg agcgagactt cgccgctgcg gatcacctcg atgcccgccg cggtgcgcag
    13261 ttgcaggcca ccgcgctgat caacgccaca ggcaatgccc tcgatggcac tgtgcagccc
    13321 gcgaatcacc accgcctggc cggcacagag gtcacgctga ttccacgcct cggcaaaggc
    13381 ggcaaaaccc tgtcgatgaa actgcgacag catcgccacc agctgattga gcagcagcgc
    13441 cgtcagccgg ttgcgcgaca gtggttcggc gaggtggcgc tgcagatcgc tccagcgttg
    13501 atcgatcgcc gcctcggctg ccgcgggcat gcgcacgttg acccccacgc cgatcaccac
    13561 ctgacagtgg ctgtccatgt cgccgacggt ctcgatcaga atccccgcca ccttgccctg
    13621 gtcgatcagc aggtcattgg gccacttcag cagcgcgccc tcaatgccaa gctgctccag
    13681 cgtgcgggcc agcgccactc cgaccgccag actcagccct tcgagcgtgc tgacgccggc
    13741 gttgaacgac cagccgagcg acaggtagag gtttccgcca aaggggctga accagttgcg
    13801 gccacgccgc ccacggccgg cggtctgccg ttcggccaga cagaccagac catggatgct
    13861 ggcggtctgg gccgcctcga ccagccggct gttggtcgaa tcggtcgacc agtgcagatc
    13921 gagtctgagt ggctggcgca gcgcctccgg atcgagctgc gcgcggatgg ctgccgcatc
    13981 gagcagatcg aggccaccgt cgatgcgata gcccttgcca tgacgccgct cgatggcaat
    14041 gccggcggac tcctgcaagc gctgcaaccg cttccagatc gccgcgcggc tgatgcccag
    14101 cagcctgccc aggacctcac ccgagtgaaa ctggccatcg gccagatgtg tcaacagttg
    14161 ccggttctgc agttcggcat cactcatgct ctgttcctga agctgaaacc caaaaagaac
    14221 ccggatggta cggcatcgcc agtggccatg acagggcggc ctttttatcg acagtgagca
    14281 gtggagcagc gctgccatgg tgaaaattca tggcgccgtc gaggggaagt cacaatcccg
    14341 ctctacccat gcgtccatga tcaccaccaa tgaaaccggt ccggatgtcg gccgctgcct
    14401 gttcacgcta ttcgaaagcg ggcaagcggg cgtgcagcgc atcgacatct ggaacggcaa
    14461 cgccacccag accctctggt gcagcccggt ggccgatggc catgcctcct tcgatgcctc
    14521 ctgctggacg cccttctcag tcaccgcacc actgtgccat caggcggcgc agtggctcca
    14581 gctcgaatgg cggctctagg cgggcattga tgccgctggc ttgccagtca ctctccggcc
    14641 ggtcaggacc gtcgatgacg accagcggcg tgggcagtcg attggactgc aactcccagg
    14701 ctcgaattct ctggccgagc tcaagggcat cgagcagtgg tggctcatgg tggatcagga
    14761 tcaggtcata atgggtctgt ttgatccgtt gcagcaggga gctgcgttcg ctgacaccct
    14821 cgacggagac cttcagtttg aggaagagct tgcgcagatg gtcgagcgtg gccggatcgc
    14881 gttcgaagat caggacccgg ctgtcggcat ccgggctgtg tgaagtcgac tcgatcgctt
    14941 tgcgcggcaa cagatcgccg agcagccgtt gcagttgcgc gccatgaatc ggcttggtca
    15001 gcaccttggc gatgcctgca tggcgcaacc gctccgaggt gggggcggcg ccgaagccga
    15061 gcagcaggat catcacggga gagggcgaca gcccttcctg gttgattttg agggccagtt
    15121 ccagccctga caacggggtg ctctcctgtt cgagcagcag aaagtcgaaa cccttgccga
    15181 ggtgaccctg gtcgcgaatc agggcgatgg cctggttgct gttgctggcc gccagggcat
    15241 ccatgcccca gcgtcggcaa cggatcagca gggtgtcgcg gatggtttcg ttgccttcga
    15301 cgatcagaat gcgctggccg gacagtcgga taccgtcggc tgccgtcgat gacgagactg
    15361 gcagtgacac ggggcttggc agggtgaagc tgatcgagct gccgcggccg gcatggctga
    15421 cgaagcgcag ttggccgccc ataccggtca ccaaccattt gacgatcggc aagcccaatc
    15481 cgccctgttc gcgcgactcc agttcgacca gcatccgatg caggggcagg tcgagcagct
    15541 gttgaatcgg ttcgggaatg ccaggcccct gatcgctgac ggtgaagcgc agaccggggc
    15601 tttcgctcag ggcctcaacg ccgatccgca cttcaccggc atgaatcgct gcgatggcat
    15661 tgctgaccag ctcgaccagt gcctgtttga tgcggcgtgg atcgccctgg gcagtctctg
    15721 ccagtctggg atcgagggca acggtcaggg tgatccgttg tggatcggca atcagacggg
    15781 tctgcgcgac cacttcatcg agcagttgtt gcagatcgaa ctcatcgaca tgaatctgca
    15841 gggtgtccga ttccagaccg acggtattct gagcccggtc aaccagatag atggcctttt
    15901 cagccgcctg gtgaatggtg cgtgcctgat ttttttcatc tttgccaaga tcggcatcga
    15961 tgagcagttc gctcatgcca tggatgccgc tcagaggatc acgcaactcc tgggtgatct
    16021 tggcgatcag cttgcggcgg gcacgaattt cgacctgccg ccgctcatcc tggtgtcgtt
    16081 cggccaggtg ccactgttcc atcaggcgga ttcgatgcag cagtgccagt gagaggatga
    16141 tgccctcgat ggctgccacc cccatcactc ccttgccggc gaggatctgg atgtcggtgc
    16201 cgccaagcgc atgcaggatc atcatcagca gcacgggcag cagcaacatg cggccacaga
    16261 agtagtaaag ggctgggcgg tagccctgtt gggcacggta gaggctgagc agcagcagaa
    16321 agagggtgaa aagtgccgaa caggcgagcg ccaactggta cagcaatacg atattggata
    16381 gaaagagtgc gctcagggca gcgagaatga caatccactc gccgaggcga ttcaaccggc
    16441 tccagcgccc ttcatgcgca tcctgcaaca gaaatacacg ggcaaagcgc agtgcagtgt
    16501 agttggtcag cagtacggca aggatcgcgc tttttcccag ccattttgca tcgatcggca
    16561 ccaggttgaa aaacttgcca tcgatcaatg cctgcaacag ggtcatcgac agcaggaagg
    16621 cgccatagta gaggttggcg ggttcccgat tggcgaagaa catcaggaga ttgaacagaa
    16681 tgaccgccgt catcagccca aagaaagtgc cggacatcca ctggaaccac tcgatcttct
    16741 gcagcagtgc attggggacc cagatggtgg ggtcgagtgc aatcggcagc gggtggtgca
    16801 ggcgcaggta gtaggtggtg gattgcgaga caggcttgat cagaaacacc tgtttcaagg
    16861 cagcgcggct gtcagagtcg tcaaatgtcg gatcaaccga tcgggggttg atgatttttc
    16921 cgctgccatc cactgcgaac agtgttgcct cgctcaatga ggcatgaccg agatggagca
    16981 ggcgattggg ttggtgcggg tcacccggtg cgatctcgaa tttgatccag gtggtggcgt
    17041 cgggtcgatt gagccacagc cgtgagcgct tcagcggcat gaattggtgc tggtaggcag
    17101 gcgtgagcag tgtccggata tcgacctgat tgctcgggtc agtgtagatc tgcaggtgcg
    17161 cggtgatgtc cgtgcaggaa gatgcctcat ccagcaccac gggctgcgcc gacagaggca
    17221 tggcgctgcc cagcagccag agcagagctg ccatcagcca gcagggccag gtcgagcgct
    17281 cccggcacat cagaacgtgt tgacgtggct ccatgccgga aggttccatc gtgggccctg
    17341 cttcactcag atacgggaag gcgtttgcgc agctccgcct tgttgatctt ctccagcgtc
    17401 gagcgcggca gttcatcgac caccatgacg ttgcgcggca ccttgaaatc ggccagctgg
    17461 gtgcggcaat ggtcgatcag cctggccgcc agatcggcgg gcgcgtcggg gttggcgatg
    17521 acaaaggcgg ccggcacttc gtcgagcatc ggatggcgct gcgccaccac tgccacctca
    17581 tcaatccagc cggtgccgcg aatcacgctt tcgatctccg aggcggcgac attctcgcct
    17641 cccaccttca gcatgtcctt gtcgcggtcg gcgaagaaga catggccatc gggcatcggt
    17701 cgcacccgat cgccggtacg gaaccagcca gcttcgtcga agctgtcgcg ggtggcctcc
    17761 gggttgtcga gatactccag aaacagcgag acaccacgaa tgccgcgaat cagcagatgc
    17821 ccggtttcac ccggatcgac cggcacgcca tcgtcatcca gcacggcgat ctcatattcg
    17881 ggcgcgggac ggccacacga aagtgatgca ttgggaaagt gcagatcgcc gacgatgccg
    17941 tgggtgatgg tttcggtcat gccccaccag ccaaggctgg tgacgccgaa gtaggcatcg
    18001 gtcggcggcg cacagatgcc gttgccccag acccgatagc tgtgccgtgg cggtcgctcc
    18061 tgctgcagca gcgccttcag gcagaaggga atcatcgagc tccaggtgca gccgtggcgc
    18121 agcgaaacct cccagaaccg gctggccgag aagcgtggct gcagcaccac ggtgccaccg
    18181 acccagagtg aaccgagcac cgaataggat tgggcattgg tgtggaacag cggcagatgg
    18241 gtgagggtga cgtcgtttga agtcagctcc aggtgacggg cattgatctg ggcgccccac
    18301 agggcgttgg catgggtcca gaccaccgcc ttggggcgtg atgtggtgcc ggaggtgtat
    18361 tggattccga ccggcgccag ggctgccgag gggtaggcgg gggccatgct gtcggccagc
    18421 agcgagccaa atggaattgc ctgatcgggc aggaattcgc tggaaagtgg tggagcaccg
    18481 gcatcattgt cggtgaccgc cagccagcga atgcctttgg cttcgcgggc aatcagatca
    18541 gcatgagcgg gttgggtaat ggcgcccacc gcttgcgaat gggcggcaaa ataggcgacc
    18601 tcgggaccgg cgctgcgggt gttggtggtg accccgaccg cacccagttc ggcgcaggca
    18661 taccaggcca gcagtgactc gggacagttg tcgaggtgaa tcagcacccg ctcggtcggt
    18721 ttgacgccga gggcggcaag accggcggcc agacggcgca cctcggcatg gaactggcgg
    18781 taactccaac tgcgggtggc gcccgagaag ggctcccaga tcagaaatgg atgatcgggc
    18841 cggacggctg cccagtgagc cagcagccag ggcacattgc gcccctgata gtggctgctg
    18901 gcgggggagc gcactgcgga gtgagtcatc gacatggtca ggctcctgct ggctgttctg
    18961 gcttcctgcg gcatcgatgc agagcgcccc gctctgtcga caccctcttt attatctatt
    19021 ctggttccag ctcttttctg cgtgctgccg aagcagctcg gcatgttgct ctgcttccag
    19081 ccgtggtccg aagcgcgaca ccacagccgc tgccgcacgg ttggccagtc gtccggcggc
    19141 ggtaaagtca tacccatggt tcagggcata gaggaaggcg cccgcgaaca tgtctccggc
    19201 gccattggtg tcaatggcat ggactggcac ggaatccacc tcgatcaggc ctgcgccatc
    19261 gtacagcagg gcacctcggg cgccacaggt gatggcgaac tgttttgcca ccctgcgcag
    19321 ttgctcggcg gcatccatca gcgattcggc gccggtgaac aggcgtgcct catgctcatt
    19381 gcaaaagagc agatcgacgc cgtttcccag catcgctgaa atgcccggct tgaaatgttg
    19441 caccatgttc ggatcggaga aggtgaggct ggtcttgatg ccatgccgtt cggcaaactc
    19501 ccgtgccgca atggctgccg cacggctgct gtcggaagtg accagatagc cctcgaggta
    19561 gaggaactcc gaccgcttca gcgcatcaaa gtcgatgtcc tcgatgtcca aggtggccga
    19621 gatgccaaga aaactgtgca tggtccgttc ggcatcggcc gacaccatta ccagacagcg
    19681 accagtcacg ccgccagccg ggcgcgcgcc attgatgttg ctgctgacgc cagcttgggt
    19741 caggtcatgc agatagaagt cacccggctc gtcatcaccg agtttgcagg cgtagtagca
    19801 tttgccgccg aaatggctga cggcaataat ggtgttggcg gcggagccgc cgctggcacg
    19861 ttttttgacg ccgtagcgct gtttcaactg tgtcagcaac tgctcctgtc gcgcatgggg
    19921 aaccagcgtc atcacgccct tgtcgatgcc ctgttcggtc aaaaaatgct cttcaacctc
    19981 aaactccttg tcgaccaggg cattgccgat gccgtagaca tggtagcgtg tcatggtttt
    20041 cctgtacttc gaatgaaaaa tctggaccag cttggcatta tcggaattgc tggcccgtac
    20101 gcaagtcgaa accggcgatc ctggctgatc acggccgctt cggctggccc ttgtgagcac
    20161 tctgacggtc gatgcagttg aggtctggcc tgatcaagag tctggtggcg ctggcggccc
    20221 tgttgacggc cttctgcgtc tatctggatg cacagatcgt cgactatttc gagcaacacc
    20281 gctaccagcc accggcgcgg gtccatgcgc ggccgctgct gctgcgtgaa ggagagctgc
    20341 tcagcgagga gcggctgacg accgaactgg cggcgctggg ttaccgtttc gttgccgggt
    20401 tgaaggaacc cggagaggcc caccgcgaag ccggtgaggt ggccttctgg caccgcggtt
    20461 ttcgcttcgc cgagggtgac gagccggccc gccggctgcg gctgcgcttt gccggcgggc
    20521 gcatcaccga gttgcggcag gaagatggcc gtgacgtcgc cagtgtgcgg ctggagccgc
    20581 tcgaaatcgg ctcgatcgag acacggctgc atgaagatcg tcagccgctg gccatcgacc
    20641 aggtgccgca accgctgatc gccatcctgc tggcggtgga ggaccgtcgc ttctttgatc
    20701 accatggcat ctcgctctgg tcgatcctgc gggccagttg ggccaacctg cgggccggtc
    20761 aggtcgaaga ggggggcagc accctgacgc agcaattggt caagaacctg ttcctgagcg
    20821 gcgaacgcag cctgtggcgc aagggggtcg aagccttgat ggcgctgctg ctcgagttgc
    20881 actatgacaa gtcgctgatt ctcgaaacct atctgaacga gatctacctc ggtcagcagg
    20941 ggccgcgggc gattcatggc gtcgaactgg ccagtcgcca ctatttcagt cgtccactcg
    21001 atcagctcaa cctgccggag atggccttgc tggtggggat gatcaagggc ccttcacttt
    21061 atgacccgtt tcgtgacccg gttgcggcgc gtggccgccg tgatgtggtg atcgagcgac
    21121 tgctggcaga gcggatgatc accccggagc aggcaaccgc ttcccgcaat gcaccgctcg
    21181 gggtcagggc gcgggagttc gccaatcagc atccggccta tctcgacctg gtgcggcgtg
    21241 agttgcaaca cgatcaggtc gacttgaaaa acaatatcaa cggattgcgt atctttactc
    21301 atcttgatcc tgaaattcag cgccaggccg agcgggcgtt gcgtgagggc atcgacgcgt
    21361 tggagagaaa acatgcgttg ccggccggga cgctgaatgg cgcggtggtg atggccgaaa
    21421 tcgacagcgg gcaggtggtg gccgtggtgg gcgatcgaca gggaacgttc tttggcttca
    21481 accgtgcgct cgacagccgc cgaccgatcg gttcgctgat caagccggcg gtctatctca
    21541 ccgccttgac gcagccgtcg cgctatcgtc tcgacacgcc gctcgaagat cagccgctca
    21601 ggctgcgcga tgccaatggt cgctcatgga gtccgggcaa ctatgaccag aagagccatg
    21661 gcagcgtgcc gctctatcgt gcgttggcca actcctacaa ccaggccacg gtgcggctcg
    21721 gtctcgacct ggggctgcca caggtgatca ggaccctgca tcaactcggt gtcgagcagc
    21781 cgattgccgc ctatccctcg ctgctgctgg gcacacttga gcgctcaccg ctggagctgc
    21841 tgcagatcta tcagaccatt gccgccggcg ggcgccatgt gccactgcat ggcgtcggtg
    21901 gcctgaccgc acagcagggc gtgcctccag cgttaccgcc ggtgagtgcc gagcagcgct
    21961 tcgacgcagc cgaagtggcg ctgctgcacc acggcctcta cgcggtggtg accgaaggca
    22021 ccgcacgtcg tctggatgag caactgggta tcgcgcggcg ggtgatcggc aagaccggca
    22081 ccaccaatga tcagcgcgac agttggtttg ccggcttcga tggccgctat gtcgccgtgg
    22141 tctggctggg gcgtgatgac aaccagccaa tgccgctgac cggggccacc ggtgcgctcg
    22201 aggtgacggc gcgactctat ggcgccatcg actcccgacc ctggcgctgg agtgcgccgg
    22261 ctggagtaca gatggcggcg attcagactg cggccgggtg ccgggagttg ccatttgtca
    22321 gtggcagcga acccgttcag tccggctgtg gcgtcgggtc acgcctcggc gccaccgctg
    22381 gcgaggccgc gcaatcgtcg cagagcacgg tcaaaggcac actcaaaagc tggttcaacc
    22441 ggttgttggg acgctgaccg ggattgttcc cgccaaccgt ccgagtgggt gcgcaatgca
    22501 caagtggtcg tcagtgccgc atgaacggcc gaatcgatcc ggaaaggatg ccgctgccgg
    22561 aatcagtcgt ccacccgctt ggcgagctgg cctttgctgt cacgaaaggg gtgactttgc
    22621 tagaatcggc cgcttgttct ttttcggcca gccccgcctg catgcttgat cgcacccgga
    22681 tcgttctgct tcgcacctcg caccccggca acatcggtgc ggccgcccgc gcgatgaaga
    22741 ccatggggct cacgcggttg ctgctggtcg atccagtgcg ttttccggca cccgaggccg
    22801 agttccgctc agccggcggt gcggacgtgc tggcgcaggc gcaggtggtg gcaacgctgg
    22861 acgacgcaat cgattcctgt cagctcgtcg ttggcagcag cgcgcgcagc cgacgggtgc
    22921 cctggccact gctcgacccg cggcaactgg cggagcggct cagccaactg ccggcgcaga
    22981 ccgaaatcgc gattctgttc ggcaatgagc agcatggcct ctccaatgag gagttgcacc
    23041 gctgtcactg gcatgtgcag attccggctg atgcggagta cggggtgctg aaccttgccg
    23101 ctgcggtgca ggtgatcagc tatgagttgc ggatgcagtg gttgcaggca ggtgacatcg
    23161 ccacggtctc gaacagctgg gatgtgccat tggcaaccag cagcgagatc gaacagttgt
    23221 tcctccatct tcagaaactg ctgctcgaac tcgactttta tgaccccgac aacccacggc
    23281 agttgctgac ccggctgcgg cggctgctgc tgcgcagtgg cctggaccgg atggaggtca
    23341 acatcctgcg cggcatcctg gcggcggcca tggcgcgcat cgaccattga aatcaaagac
    23401 tggacttgcc catgttgacc cgactgcgtg aagagatcga ttcgatattc gagcgcgatc
    23461 ccgcagcgcg caccctgtgg gaggtgctga cctgttatcc ggggctgcat gcggtgctgt
    23521 tgcatcgagt gagtcactgg ctctggcagc accgattgaa atggctgggg cgttttcttt
    23581 cgaccctgac ccgttggctg accggcatcg agattcatcc cggcgcccgc atcggtcgcc
    23641 gcttcttcat cgaccatggc atgggtgtgg tgatcggtga aactgccgaa attggcgatg
    23701 actgcacggt gtatcatggt gtgaccttgg gcggcaccag ttggcaaccg ggcaagcgcc
    23761 accccacgct gcgggatggg gtggtggtgg gtgccggggc caagattctg gggccgatcg
    23821 aagtcggtgt cggggcaagg atcggctcca atgccgtggt caccaagaat gtaccgtccg
    23881 aggccacggt cgtcggtgtg ccgggccgca tcattcagcc gttgacgggt gagcctccct
    23941 cgacctcttc accgcaccgc gaagccatcg ccagaaaatt cggcttcgat gcctatgggc
    24001 tgggacgtga tgtgcccgat ccggtggcgc gctcgatcca tgccatgctc gatcacattc
    24061 atgcgctcga caccaagctc gatggcatct gtggcgccct gaaggcgctg ggcagcgatc
    24121 actgcgccga gacgatcgag attctggatg aggtgatcga cctggaggga ggtgtgccgc
    24181 ccggcgccag cggtcgagcc aatacttgac ttgtaaagta ggtttttgga tactcatttc
    24241 acctatcgac gaagttgcct ggagctgatg ccatgaggtt gaccaccaaa ggacgatatg
    24301 ccgtcaccgc aatgctcgat ctggccattc atgccattga aggtccggtc agtctggcag
    24361 acatttcaga gcggcaggag atttcgctct cctatcttga gcagattttt gccaggctgc
    24421 ggcagaacaa gctggtttcc agtgtcagag ggccaggcgg cggctatcgc ctgagccgtg
    24481 atcggcagga gatctcggtg gtacagatca tcgatgcggt caatgaatcg atcgactcca
    24541 ccaactgtgg tgggcagggc aactgccagc gtggcgaaca gtgcctgacg catcagttgt
    24601 ggtgtgacct cagcgagcag attcacctgt tcctggctgg catcaccctc gcacacctga
    24661 tcgagaagcg tgaagtgcag gacattgcca agcggcagcg tgacttcatt gcccgagagc
    24721 gtggcaccga atcgctggcc catattcgtc tgggcaccag ctgagcgact acagcccgga
    24781 tattgacgag cgaggagcag accaacatgc gcgtacctat ctacatggac tatgcggcga
    24841 ccacgccggt cgatccgcgc gtcgctcaga agatgagcga atgtctgttg atggatggct
    24901 gttttggcaa tcccgcgtcg cgcagccatc agtttggctg gaaggccgag gagatcgtcg
    24961 aaacggcccg acggcaggtg gccgatctga tcaacgccga tccgcgcgag atcgtctgga
    25021 cctcgggggc caccgagtcg gacaacctgg ccatcaaggg tgttgcacac ctctatggcg
    25081 agcagggcag gcacatcatc acctcgaaga tcgaacacaa ggccgtgctc gacacctgtc
    25141 gccagctgga gcgtgaaggg ttcgaagtca cctatctcga tccggattcg accgggctga
    25201 tcactgccga gcaggttggc caggcgctgc gtgatgacac cattctggtg tcgatcatgc
    25261 acgtcaacaa cgaaatcggc accatcaacg acgttgccgc cattggcgag gtgacgcgcg
    25321 cgcgcaaggt gctgctgcat gtcgatgcgg cgcagagcgc gggcaaggtg gagatcgatc
    25381 tggagcggat gaagatcgat ctgatctctt tctcggcgca caagatctat ggccccaagg
    25441 gtatcggcgc gctctatgtg cgacgcaagc cgcgggtgcg tctcgaagcg cagatgcatg
    25501 gcggcggcca tgagcgtggc atgcgctccg gcaccctggc cacccatcag attgccggca
    25561 tgggcgaagc gtttcgaatc gccaaggagg agatgggagt ggaatgcagc cgcatcgccg
    25621 cgctgcgtga ccggctgtgg caagggatca gcagcctcga tgcggtccac ttgaatggcc
    25681 atgccgaacg gcatgtctgc ggcattctca acgtcagctt tgcctgcgtg gagggcgagt
    25741 cgttgctgat gtcgctcaag gatctggccg tctccagtgg ttcggcctgc acttcggcca
    25801 gccttgagcc ctcctacgtg ctgcgcgcgc tgggattgag cgatgagctg gcacacagtt
    25861 caatccgttt ttcgattgga cgcttcacca ctgaacagga gatcgaccat gcgatcgagc
    25921 aggttcgcaa ggcggtgacg cggctgcgtg aactctcccc gctgtgggag atgcaccagt
    25981 cgggtgttga tttgagcacg gtccagtggg ccgcacactg actgctgctg gcggcggctg
    26041 agcggtcagg cacggcttca tcatcggaga gatcaaaatg gcatacagtg aaaaagtact
    26101 cgatcactat gaaaatcctc gcaatgtcgg cagcttggac gtcaatgacg tcaacgttgg
    26161 caccggcatg gtgggggctc cggcctgtgg tgacgtcatg cggttgcaga tcaaggtcaa
    26221 cgaggcaggc atcatcgagg atgccaagtt caagacctac ggctgtggct cggccatcgc
    26281 gtcaagctcg ctgctgaccg aatgggtcaa gggcaagagc ctgaacgaag cggcgcagat
    26341 caggaaccag gagatcgccg acgagctggc actgcctccg gtgaaaattc actgctcggt
    26401 gctggccgaa gatgcgatca aggccgccat tgccgacatc aagcagaaaa gaggcgaata
    26461 gcgccatgcg ggcagacgat cgaggtgggt catgggcatt caactgactg aacaggcggc
    26521 tgaacacatt cgccagcaac tcgatgcgcg cggccatggc attggtgtgc gcatcggcgt
    26581 cacgccgagc ggctgttccg gcatgtccta tctgctggag ttcgtcgacc agctcaatga
    26641 agaagatcag gtgttcgaga aagatggcgc caaggtgatc atcgatgcca agagccttcc
    26701 ctatctggag ggcacccagc tcgacttcgt gcgcgagggg ctcaacgagg gtctgaaatt
    26761 caacaacccc aatgtcaagg ccgaatgcgg ctgcggcgag agtttcactg tctagtccac
    26821 ccgccaccgg cggtcctggt cttctgttgt catgcgctcc tattttcagc tctttgatct
    26881 gccgcagtgt tatcagctcg acctcgagcg attggatcgc aactaccgaa acctgcaggc
    26941 ctcggtgcat ccagaccgtt ttgttggcga tggggcacag gccaggcgtc tggccgaaca
    27001 gtacagcgga ttgttgaatc aggcctacac gacgctcaaa tccccggtcg agcgcgccgc
    27061 ccacctcttg cagttgctgg gcgttgaaat cgatggagca gccaccatct ctgatggtga
    27121 ttttcttttc aggcagatga cgctgcgcga aacgctgcac gacgtgcgtc aacaacggga
    27181 tgaaacagcc ctgttgcagc tcaggcagca ggcactggcc gaacagaacg ggctgcagca
    27241 ggccttttcg cgggccatca aggatccgca aaatttgcaa tccgccttgc aattcttgct
    27301 caagatgcgg ttttttcaaa agtttctggc tgacattcat gatgttgaag atgtgctgct
    27361 tgattagtcg ccgagcgttg acaagcgctg ttttcgccaa attcgagtag cccatggcac
    27421 ttctcaagat agcggaaccc ggtcaatcca cgcctgcggc tcgtcctcga cggcgagcgg
    27481 tcggcattga ccttggcacc acctactcat tggtggcgac cgtcgaagcg gggcaggtca
    27541 aggtgctgag cgacagtgat gggggggtcc tgctgccatc ggtggtccat taccgtgccg
    27601 aggggctgcc cgaggtgggt gtggccgcgc agtcgctggc gatcgttgat ccactcaaca
    27661 ccatcgcctc ggtcaagcgg ctgatggggc agggcatggc ggcagcgcag cggctgtcgg
    27721 accatccgct ggaggcgagt gccaatggca tgccctatct gcgaacggca gcgggcaaca
    27781 agagcccggt cgaggtgtcg gccgagatcc tgcgcgcact tgcggagcgt gccgaacggc
    27841 acttcaacga aaccggtgtg gccgcggtca tcacggtgcc ggcctatttc gatgatgcgc
    27901 agcgacaggc gaccaaggat gctgcgcggc tggctggcat cgaactgctt cgactgctga
    27961 acgaacccac cgccgccgcg attgcctatg ggctcgatca gaatgccgaa ggtgtgattg
    28021 cggtctatga cctggggggc ggtaccttcg acatctcgat tttgcgcctg tcgcgcggtg
    28081 tcttcgaggt gctggccacc ggtggcgaca gccagctggg cggcgacgac ttcgaccggc
    28141 gactggcgca gtggcttctg agcgaaacgg gtcaccccga acccttgacg ccggccgaat
    28201 cacgtcacct gcttggcgtg gcccgtgctg ccaaggagcg cctcagtgaa caggtgcaga
    28261 caacggttga attgaacgaa ggcaacctgt gctggcaggg tgtgctgacc cgcagccagt
    28321 tcgagcagct cattggtgac gaggtggagc gcacgctgcg cgcctgtcgt cgggcgttgc
    28381 gtgacagcgg ttgtgagttg aaacagatcg acgaagtggt gatggtcggt ggatcgagtc
    28441 gcgttccggt ggtgcgcgag cgggtggaag cgctctttgg gcgcacaccg cgcatcgaca
    28501 tcgatccgga gcgggtggtg gcgatgggtg cggcgcgtca ggccgatctg ctggccggca
    28561 atgcgcaaca gcaggccctt ctgctgctcg atgtcattcc gctgtcgctc ggcatcgaaa
    28621 ccatgggcgg cctggtcgag cggatcatcc cgcgcaattc gccgattccg gtgtcgcggg
    28681 cccaggagtt caccacccat caggatggtc agactgccat gtcgatccat gtggtgcagg
    28741 gcgagcgtga cctggtcgag gagtgccgtt cactggcccg cttcgtgttg cgtggcattc
    28801 cgccgatgag cgccggcgca gcacggatcc gggtcaccta tcaggttgat gccgacggac
    28861 tgttgagtgt ttcggccacc gagctgacca gcggcgttgc cagttcgatc gaggtgaagc
    28921 cctcctacgg attgggcgaa gaggagattc tgcgcatgtt gcgtgactca cgcgccttcg
    28981 ccgccgagga catgcgcgcg cgcgccctgc gcgaacagcg gattgccgcc gatgccctgc
    29041 tgcaatcgct cgaccatgcg ctgacgcaag atggcgacaa attgcttgcg gcgaccgaac
    29101 gccagcagtt gctgcagcag atggcccggc tgcgcaccct gatggcgggt gatgatccgc
    29161 agcagattgc gctggccgtc gagcagttga accgtgaaag ccagagtttc gccgagcggc
    29221 gcatggacct gatcctgcgc agtgcgctgg caggcaaatc gatcgaggag gtcaagggct
    29281 gacatgccaa ccatcaaggt cttgccccac ccgcagcgct gcccgcaagg ggcactgttt
    29341 gaagccaagc ccggcgacaa cctgatcgag ctgctgctgg cgcatgacat cgaaatcgaa
    29401 catgcctgcg agcgctcctg cgcctgcacc acctgccatg tgatcattcg acagggcttc
    29461 gactcgctgg atgaagcagc cgatctggaa gaggacatgc tcgacaaggc ttggggcctg
    29521 acgctgactt cgcggctgag ctgccaggtg accgtcgggg agcaggatct gctggtcgag
    29581 attcccaaat acaccatcaa tctcgcctcc gaaaatcatt gaggtgaccg acatgaaatg
    29641 gaccgatgtt cagcagatcg ccatcgcgtt gacggaacgg catactgatg tcgaccccca
    29701 gtatgtgcgc ttcaccgacc tgcaccgctg ggtctgtgag ctcgagggtt tcgctgacga
    29761 tccgctgcgc tccaacgaga agattctcga ggccattcag gccgcgtggc tcgatgagtt
    29821 gtgaatttct gggccaactc cgcgggcctc gctcagagag acggccactt taggctataa
    29881 tccgcccttc gcgcagtcgt actggtgagc gcgaaccttt cctaccgttt ttaccctgga
    29941 gacacacacg tggctgttga acgcacgctt tccatcatca aacccgatgc cgtcggcaag
    30001 agtgtcattg gcaaaatcta cagccggttc gaaaaggcag gcctcaggat cgtggctgcc
    30061 aagatggtgc atctgagcaa tcgtgaagcc gaaggctttt acgccgaaca ccgcgaacgc
    30121 cccttcttca aggatctggt cggcttcatg acctccggtc cggtgatggt gcaggtgctg
    30181 gaaggcgaga atgcggtgct gctcaaccgt gaactgatgg gggccaccga tcccaagaag
    30241 gcggacgcca acaccatccg cgccgatttc gcccagtcga tcgatgagaa tgcggtgcat
    30301 ggctccgact ccgcccaatc ggcggcacgg gagatcagct acttcttcgc cgccaccgaa
    30361 ctctgcccac gcacccgctg agcagttgtc tgtcgagtca cgtcatgggt gaaaccacca
    30421 ggatcaatct gctgggcctc tctcggccga agctggagca gttctttgcc gagctcggtg
    30481 agaagcgctt tcgtgtcgac cagacactga agtggctgca ccaccgccgt gccgacagct
    30541 ttgatcagat gagcgacctc tccaaggggc tgcgtacgcg ccttggagag gtggccgaga
    30601 ttcgcccgcc cgaggtggtc catcggcagc ggtcgctcga tggcacccgc aagtggttga
    30661 tccgggtcga gagtggcagt tgcgtcgaga tggtcttcat tccacagaat ggccgcggca
    30721 ccctctgtgt ctcctcgcag gtgggttgcg cgctcgactg cagcttctgt tcgaccggca
    30781 agcagggttt caacagcaac ctcaccgctg ccgagatcat tggtcaggtg cgcattgccg
    30841 tcgatgaact ggccgggctc tatcccgaac gtgaccgtgc cgtgaccaat gtcgtgctga
    30901 tgggcatggg cgagccgctg ctcaacttcg acaatgtggt cgatgcagtg cagttgatga
    30961 tggatgactt cggttacggc atctccaagc gccgcgtcac cctcagcact gccggtctgg
    31021 caccgatgat cgaacgcctc ggcgaggtga ccgatgtctc gctggcgatc tccctgcatg
    31081 cgacgaccaa tgcactgcgt gaccagttgg tgccggtcaa tcgcaaatat ccgatcgagg
    31141 tgctgttcgc cgcctgtcag cgctactttg cgcgtctcgg cgaacaccgc tcgctgacga
    31201 tcgagtacac gctgctcgat ggcatcaacg accaaccgga acaggcgcgt gagctggctg
    31261 tactgagccg gattctgccg agcaagatca atctgattcc gttcaacccc tttcccaaca
    31321 gcggttaccg ctgctcatct ccggcggcca tcgacgcttt tctgcagatt ctgcaacgtg
    31381 aagggtgccg ggcgaccatt cgcgccaccc gtggcgatga catcgatgcc gcctgcgggc
    31441 aactggtggg gcaggtgagc gaccgcaccc gccggcagca gcgccatgcc cagcgtcatg
    31501 cagctgaact ccttgcgcaa ccactcaccc tgcggtgatc gatgatgagc gctcctgttg
    31561 tctcctgctg tcgtcgactt ctcgtcacgc tgctgctggc gttgctggca ggctgtgtca
    31621 ctcaggagac caccccgttc aacaagcgtg accaggccaa ggcggtcgag gcctacatcg
    31681 cgctctccat cgcctacctg cgcgacggca atgcacagat ggccaaggag tcgctgcaaa
    31741 aggccaaggc gctcgatccg gacaatccgg cactgttttc ggcgaccgcc tacctgttcc
    31801 agtggcaggg tgaaccgcaa ctggccgaag agaattatcg caaggcgttg agcagaaacc
    31861 cgcaattctc cgaggcgcgc aacaactatg gcgtctttct ctacagccag gggcgctatg
    31921 acgaggcgat cagcgaactg ctgaaggtgg cggaagatcc gctttatgcc cagcgccatc
    31981 aggcttatga gaacctcggc tactgcgcgc tgaagaagaa cgacagcgca ctggcgatcc
    32041 attacttctc caaggcgctg aaaataaatg aaaaccagcc caatgcgctg ctggagatgg
    32101 ctgatctgat gttgcaggcc ggcaacctgt cggcggccaa tgcccacatg gggcgctttc
    32161 gcgacttgct taagatgcgc caggccgaag cgagcgcccg cagctactgg atcgaactgc
    32221 aactggcgcg ggctcagggc aaccacgatc tggaggccag caacgccctg aaactgaagg
    32281 ggctgttccc cgactccgaa gagtatcgca gctacctgaa gcagagcggg cgtgttccct
    32341 gagccctgtt ttgtccattc attcgctccc gctggaattc aacctcgatc atgcagccgc
    32401 aacagagcat cgtccggcgt aaatcgcgca aaatctgggt cggttcggtg ccggtcggcg
    32461 gcgacgcgcc gattgccgta cagagcatga ccaacagcga cacccatgac gtcgcagcga
    32521 cggtggcgca gattcaggcg ctcgccgatg ccggcgccga catcgtgcgg gtgtcggtgc
    32581 cgaccatgga ggcggccgag gcctttcgcc agattcgcgc ccaagtggtg ctgccattgg
    32641 tcgccgacat ccatttcgac cacaagatcg ccctgagggt gctggaacat ggtgtcgact
    32701 gcctgcgcat caacccgggc aacatcggca aggaggagcg aatcagggcc gtggtcgatg
    32761 ccgcgcgcga ccgacgggtt ccgatccgca tcggcgtcaa cgccggttcg ctcgagcgtg
    32821 acctgcaacg caagtatggt gagccgactg ccgatgccct ggtcgagtcg gccatgcgcc
    32881 atgtcgaaat tctcgatcgg ctcgatttcg ccgacttcaa ggtgagcgtg aaggcttcca
    32941 acgtgatgat ggcggttgcg gcctaccgca agatcgccag ccagatcgag cagccgctgc
    33001 acctgggcat taccgaagcc ggggggctgc gttccggcac ggtcaaatcg gcgctgggca
    33061 tcggcaccct gctgatggag gggattggtg acacgctgcg ggtttcgctg gcggcggatc
    33121 cggtggagga ggtcaaggtc ggtttcgaca tcctgcgcag tctgcaactg cgctcacgcg
    33181 gcatcaactt catcgcctgc ccgagctgct cgcggcagaa cttcgatgtg atcgccacga
    33241 tgaatgcgct ggagcagcgc ctggaagatg tgcgggtggc aatggatgtg gcggtgatcg
    33301 gctgctatgt caacggtccc ggcgaggcgc gtgaagtgga gatcggcgtc accggggcgg
    33361 caccgcagaa cctgatcttc atcgatggca agcccagcca caaggtggca gccgaggcct
    33421 tgaccgacga actggagcgg ttgatccgtg agcgggccgc gcagaagagt cggcaggctg
    33481 ccaacaccat cgcccgcggc tgagatgctg ttcggatttt taagataaag aaataatgaa
    33541 aatcaatgca atacgcggga tgaatgacat cctgccagaa gaaacgcctc tatggcgtta
    33601 tgctgaaaaa ataattcagc aagttctcac cacttacggc taccgtgaaa tccgcctgcc
    33661 gctgctggag ccgaccctgc tgttcgaccg ttcgatcggt gaagtcaccg acatcgtcga
    33721 gaaggagatg tacacctttc ccgaccgcaa cggcgagtcg ctgacgctgc ggcccgaagg
    33781 caccgccggc tgcgtgcgcg cctgccagga gcatggcctg ctgttcaacc agacccagcg
    33841 gctctggtac caggggccga tgttccgtta cgaacggccg cagaaggggc gctaccggca
    33901 gtttcatcag atcggcgtcg aggccttcgg ctttgccgcc cctgaactcg atgccgaact
    33961 gctgctgctc agtgccgaac tgtggcggca gttgggtctg accgaactgg tcacactgga
    34021 gatcaactcg atcggcagtg ccgaggcgcg tggccgctac cgggccgatc tggtcgccta
    34081 tctgtcgacc cgcaaggaaa agctggatga tgattcgcga cgccggctcg attccaatcc
    34141 gctgcgcatt ctcgacagca aggtgcccga aacacaggca ctgctcgatc aggcaccggt
    34201 catcaccgac tacctcgatc agccctcgcg cgaccacttc gcgcggctgc gggcgctgct
    34261 cgatgcgatg cagattccct atcgcatcaa cccgcggctg gtgcgtggcc tcgactacta
    34321 ctgcgacacg gtcttcgagt gggtcaccag cgccttgggt gctcaaggca cggtctgtgc
    34381 cggcgggcgt tacgatgggc tggtcgagca gttgggtggc aggccgaccc cggcggtggg
    34441 catggcgatg gggctcgaac ggctgatcct gctgctcggc agccgcacgc ggcaacccgg
    34501 cgaactcggc ccgcacagtg acctctatct gatcgcggcc agcgacgccg agctcccggc
    34561 agtgatgcaa ctggccgagc ggctgcgccg ggccttgccg caacgggtca tcatcaccca
    34621 tctggagggc ggcagcttca agagcaggat gaagcgggcc gacaagagcg gtgccgagtt
    34681 cgcattgctg ttgggtgagg atgaactgcg tgatcaggtg gtgagcctca aaccgttgcg
    34741 cagcgaagcg ccgcagcagc ggttcgacga ggcgacgctg atcgaccatt tgcgccagca
    34801 cttcagggtg acgctataat ccggcccatt ttgactgact ggctgcactc ctttcggccg
    34861 cgatccgagg atgccgacgt cgaccgatcg atcacaggcc gctggtggcc gatgatcgaa
    34921 caacccgcat ccttcgctga gttgaactgg agaacagtgt ggacccctac ctgagtgagc
    34981 aggagcagat cgaggcaatc aagagctggt ggcgccggca tggcagcacg ttgctgatca
    35041 cgctggcgct gttgctggcc gcctactggg gctggcagct gtggcagaaa cagcaggccg
    35101 attcacaggc cagggcggcc gccctctacc agaacatgga gcaggcctac ctgctggcgc
    35161 agaagggtga aaaagaggag caggcacagc agctcgccac cttcactcat ctggccgggc
    35221 agctcaagag cgactatccg acctacggct atgcccgctt tgccgcctgg atgctggcca
    35281 aggcggcggt cgaccggcag gactatcagg ccgccgagaa ggagctggag tgggtgctgg
    35341 cccatctcgg catgggctgg atgaagggtg aggacaccct ctccgccgcg atcagccggt
    35401 tgcggctcgc ccagctcaaa ctggcgcaaa agcagtttga cgccgcgctg gcacagctcg
    35461 atctggtcag cgacggcgcg ttcgaggtgc gcgaggcgga actgaggggg gatgttctga
    35521 tggccaaggg agatgcggcc ggcgcggtcg ctgcctacac ccgggcactg gcggccaccg
    35581 cggtcgacga cccgctgcgc gggctcaccg aaatgaagct ggacgatgcc aaatcgcggt
    35641 tgccaaaaac caatacggag cagaaaccat gaacaacctt gccagagctc tgccactgct
    35701 gctgctctgc ctgctggccg cctgctccag cgacagcaag aagaagatca acctgccgac
    35761 gccgctggtc gatgtcggca acgaggtggc gctggatgag aaatggtcac gcagcatcgg
    35821 caagatcaac gtcacctcct acaaccggat tcaactggcg cttgatcagg gcacgctcta
    35881 tgcggcaacc atcgatggca ccgtgatcgc ggtcgatgca accacgggtg aacggcgctg
    35941 ggaggtcgag accaaggcgc gaatcagcgg tggcgtggcg gctggccgcg accgggtgct
    36001 ggtgacgacc ctcgatggcg agctgatcgc cctttcgagc aacgatggca gtgagctgtg
    36061 gcgcagcgcg atcaagagcg agctgctctc ggtgccggcg atcaccgccc aggccatcgt
    36121 ggtgcagggt gtcgatgacc ggctgcatgg actcgaaccg accagcggcc gggagatctg
    36181 gagccatacc gtgctgacgc cggtattgac gctgcgtggc acggccagcc cggttggcaa
    36241 tgatcaatcg gtcattgccg gttttgccaa tggcaagctg atggcgttcg atgccggcag
    36301 tggcgtgtcg cagtgggagg cagtggtggc ccaggcaaag ggccgctccg aactggagcg
    36361 catggtcgat gtcgatggta cgccactgct gcttgaacat ctgctgttcg ccgccagcca
    36421 tcagggccgg atcgctgctt tctccgccgc cgatggcaag ccgctgtggg cacaggaggc
    36481 ctccagcttc cagccactgg cgggtgatgc cgaacggatc tatctggtca gcaatgatga
    36541 cgaagtgcag gcgtttgccc tcgagtcggg caaaccattg tggaagcagc cggcactgca
    36601 ataccgcaaa ctgtcgccgc cgacccactg gcagcagtgg ctggtcgtgg ccgacagcga
    36661 aggctatctg catctgctgg ctgccagtga tgggcacatc gctgggcgct accgtgccag
    36721 ccgtgatgcc attggcgcga tgatgattgc cgatggcgaa cagctctacc tgctggatga
    36781 tggcggcaag ttgcgggcgc tggggctgaa ggcacgatga ttccggtcat cgccttggtc
    36841 gggcgaccca atgtcggcaa gtcgacgctg ttcaatcggc tgacccacag ccgtgatgca
    36901 ctggtggcca atctgcccgg actgacccgc gaccgccgct acggcgaggc gctgctgcaa
    36961 ggtcaaagaa caatcctgat cgacaccggc ggcatcgacg gcgaacgcag tggcgttgat
    37021 gccgctgccg aggcccagtc acgccaggcg atcaaggagg ccgatctggt gctctttctg
    37081 gtcgattgcc acgccggcct caccgccgcc gacgaggcgg ttggcgcgga gctgcggcgc
    37141 cttggcaaga agacgctgct ggtggccaac aagatcgatg gcgccaaccc cgacctgatc
    37201 ctgagcgagt ttcacgcgat ggggctcggt gatccgcagc cgatcgcggc ggcccatggt
    37261 cgtggcctgc agtcgctgat cgaagccgtg ctggagcgat tgccgcccca gcccgaagag
    37321 cccgaaaccc cgccagccga gaccggggtc aagattgccg tgctcggccg ccccaacgtc
    37381 ggcaagtcga cactggtgaa ccggatgctg ggcgaggagc gggtggtggt gttcgaccag
    37441 cccggcacca cccgcgacag catctacatt ccctacgagc gccacggccg gcgttacacc
    37501 ctgatcgaca ccgccggcat ccgccgacgc aagaacatcg acgaagcagt ggaaaagttc
    37561 tcgatcgtca aggcgttgca ggcgatcgag gatgcgcacg tggtgctgct gctggtcgat
    37621 gcgcgcgaga atctggtcga ccaggatctg cacctgctcg gcaccgcgct ggaggcgggc
    37681 agggcggtgg tgatcgtcgt caacaagtgg gatggcctcg accccgagca gcgggcgcgc
    37741 atccgcagcg aactggagcg ccggctcgcc ttcatcgact tcgcccgcat ccacttcatt
    37801 tcggcgctgc atggcaccgg cgtcggcgaa ctctacaaat cgatcgatga agcctaccgc
    37861 gccgccaccc gcaagttgca gacccacaag ctgacgctgc tgttgcaggc ggccgtggtg
    37921 cagcatccgc caccggtcgg tggcagcggc cgtcgcccca agctgcgcta cgcccatgcc
    37981 ggtgggcaga atccgccact gatcgtgatc catggtaatc aggtcgactc gattccggag
    38041 agctaccggc gctatctgga gcgcttcttt cgtgcccggc tgcacctgag cggcacaccg
    38101 atcaaggtgg aattccgtgg cggtgagaac ccctatgccg agcgcaagaa cacgctgacc
    38161 gagcggcagc ttcgcaagcg ccgccggctg ctggagcatg tgaaaaagcg ctgaagctag
    38221 ggcggcggca gaaacagtgc cggatcgacc ctgaccccgt tgagactgac gccccagtgc
    38281 aggtgtggcg ccgtggcccg gccgctcatg ccgacccgtc caagggtgtc gccagcctgc
    38341 accgatgcac cgcgctgcac atcgacctgc tccagatgac agtagagcgt gaccaaccca
    38401 tggccatggt cgatcagcac cgtcttgccg ttgaagaagt agtcgccgat ttcgatcacc
    38461 cggccggcgg ccggtgcctg gatggccgtg cctctgggcg cggcgaggtc gaggccactg
    38521 tgtggctggc gcggttgtcc attgaaaaag cggcgcacgc cgaaggtgcc gctgcgtggc
    38581 gcctcgaccg gcgctgccag cgtgaagacc ggcaacgtcg ggctgctcca gtggtcgaag
    38641 gcggcatcga tctgctgccg ctcccgctcg atgcgcagca gatcatccgg caacggcgtc
    38701 acctgccgtt gattggtcac ggtgatgtgc tgctcgggat agtgggcggg tccgatggtg
    38761 aagtcgatct gtcggcccgg ttcgatctcc agccgttgcg gacccacctt ggcgtcgagc
    38821 ggaatgccga ccaccgccag ccagcgcgcc gaggtcggat ggccggtcag cgcggcggag
    38881 accaccatca cccgccgtcc ctgatagcgt gcgacgggtg gctgggttgc cgcctcatgc
    38941 ccgggcagtg gcaacagcac aacgccaccc ggaatcgccg cggcgcgcgg cagcgcggcc
    39001 agtggcgagg cgttgccgag cagtaggccg atccagaggc agccggtcac gatggcccat
    39061 gtcggcgctg gaggcttcat gcgtcctgta cctcgtccgg ttctgattcg cgctgctcga
    39121 cccggcacag cagtcggccg cgatgcagcc gcaccgccac cggctggccc ggttcgacct
    39181 gatcggcgtc gcgcagcagc aggccatcgc cggcgcggct ggcgatggca tagccgcgcg
    39241 ccagcgtctg caacgggctg acggcattca gcgtcgccag cagttgctcc agccggtgtt
    39301 gtcgctgatc cagccgctgt ctgagggcct gtcgcagccg ccgctgcaat gcctccaact
    39361 ggctgccgtg ctggtcgagc cgtctgttgg gcaactgctg ccagagccgg ccattcagca
    39421 gctgcaggcg gctctcgctc tgtcgcagtt gccgcagcag gccatgccgc agtcgcagct
    39481 ccagctcatc gacccgctgc gcctgttcgc gcagtcgttg tccaggatgg cgcaggcgcc
    39541 gctccagcat cgtcagatcg gcctggtgcc gttgcagccg ctgccgcatc gcgccctgca
    39601 ggcgttcgag gtggtgcgtc aatgtggcca gcaacgcctc ctggctcggg ctgatcagtt
    39661 cggccgccgc cgagggcgtc ggcgcgcgca ggtcagccac cagatcggcc agcgtgaagt
    39721 cggtttcatg ccccaccgcg ctgaccaccg gcagtcggct gtggaagatg gcccgtacca
    39781 gttgttcgtc gttgaaacac cagagatctt cgagcgagcc gccgccgcgg gcgagcagga
    39841 tcaggtcgaa ctgatcggcc agccggttgg ccgtggcgat ggccgcgatg atctcacccg
    39901 ctgccgcggc accctgcacc gccaccggca gcagtgtcac ctcgatggcc gcaaagcgcc
    39961 gcttcagcac cgtgaggatg tcatggatcg ccgcgccact cggcgaggtg atcaccgcga
    40021 tgcgctgcac gaaggctggc aaaggccgct tgcgtgcgga gtcgaacagc ccctcggcca
    40081 gcagcttctg cttgagcagt tcgagccggc ggcgcagcgc gccatcgccg gctggctcca
    40141 gcccctcgat gatcagttga taatcaccgc gcgcttcgta gagactcagc cgtgcccggg
    40201 ccaccacccg gtcaccctcg gccggattga aaccgaggct gcgcccgcgg gtgcggaaca
    40261 gcgcgcagcg cacctgggcg cgctcatcct tcagcgtgaa gtagagatgc cccgaggcgg
    40321 ggcgtgacag gttggagatt tcaccctcga cccagaccag cgcaaaggcc tcttcgagca
    40381 gctcgcgcgc caggctgttg agctggctga cgctcaggat cggcggggtt ggacggctgg
    40441 gtggggtgac ggacgacatg gcggtgatga tgaggtgacc ctgcgcgcga caaacgcgca
    40501 gggtccgggc agatcagacg atcttcgaat cgcggttgcc ccagtagcga tccttcagca
    40561 gccgcttgta gagcttgccg gtcggatggc gcggcagctc atcggtgaag tcgatcgagc
    40621 gcgggcattt gagatgcgag atgttgtcgc ggcagaactg gatcagctcc ttctccagtt
    40681 cggggctggc caactgcgga ttcatcagct gcaccaccgc cttcacctct tcgccgaact
    40741 cctcgttggg cacgccgaac accgcgcagt cagccacctt ggggtgggtc accagcaggt
    40801 tttcggtctc ctgcggatag atgttgacgc cgccggagat gatcatgaac gccttgcggt
    40861 cggtcaggaa gaggtagccc tcttcgtcga ggtagccgac gtcacccaat gtgctccagt
    40921 cggtgttgga ggggtggcgc gactcggcgg tcttcttcgg atcattgtgg tactcgaagg
    40981 tggcgccgcc ctcgaagtag atggtgcccg actgcccggt cggcagctca ttgccctcgt
    41041 catcgcagat gtggatcggc atgccgtagc tcttgccgac cgagcccttg tgcgccagcc
    41101 attcggtcga ggtgatggcg gtgccgccat tgccctcgga gccggcgtag tactcataga
    41161 tcaccgggcc ccaccactga atcatctgct ccttgaccgg caccgggcag ggcgcggccg
    41221 catgcacggc gcacttcagc gacgagaggt catacttcgt gcgcacctct tcgggcagct
    41281 tcagcatccg cacgaaatgc gtcggcaccc attgcgaggc ggtcgccttg tatttctcga
    41341 tcagctgcag cgcgttttcc ggatcgaact tctccatcac gatcacggtg ccgccgacgc
    41401 gatgcgtcgt catgcaccag cgcagcggcg cggcgtgata gagcggcgcc ggcgacaggt
    41461 agaccgtgtc cttgttgaag ccgaacaggc cgacggcgag ctgcaccagc gaattggcga
    41521 agtcgatcgg ctcgtccttc agttcgagct tgatgccctt cgg
//
LOCUS       DAORCQ010000007        40697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_479_length_40697_cov_6.563161, whole genome shotgun sequence.
ACCESSION   DAORCQ010000007 DAORCQ010000000
VERSION     DAORCQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 40697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40697
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_40697_cov_6.563161"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKI23_01250"
     CDS             <1..287
                     /locus_tag="PKI23_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014591656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HNN35596.1"
                     /translation="LLVRALSMGKLHHAMMGTCAVAIGTAAAIPGTLVNLAAGGGERE
                     AVRFGHPSGTLRVGAEARQENGEWVAKKAIMSRSARIIMEGWVRVPEGSF"
     gene            complement(299..928)
                     /locus_tag="PKI23_01255"
     CDS             complement(299..928)
                     /locus_tag="PKI23_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005744343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNN35597.1"
                     /translation="MRRSVKLYDDPRSGNGYKVRLLLAQLSQPYQYLPIDILRGESRT
                     PAFLKKNRNGRIPLLELEDGSCLPESNAILCFLSQGTPFWPTERLEQAQVMQWLFFEQ
                     YSHEPNIATPRFWLVIKGFEPTPFNLELLAQKQKAGNQALAVMDEHLQHRQFMVAERY
                     TIADIALYAYTHEAHEGGFDLSRYPAVLAWLQRIRAQPGHITIDQWTAG"
     gene            complement(993..1688)
                     /gene="pgl"
                     /locus_tag="PKI23_01260"
     CDS             complement(993..1688)
                     /gene="pgl"
                     /locus_tag="PKI23_01260"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNN35598.1"
                     /translation="MVREHRFADSTQLVAALTQSIVADLQQALDQRAAATLLVSGGRT
                     PTALFQRLSQQPLPWSRIIVSLTDDRWVPETHPDSNAGLVRRELLRAAAAAARFVPLV
                     TAAADPWLGHAESERLFAQLHRPFDAVILGMGTDGHTASLFPGSPQLQTGLHEQHSAT
                     FAAMPPAGVDHPRVSLTLATLLDSRQLYLHIEGEQKWQRYLQARQPGAVETCPVRAVL
                     EQQRVPLDLYWSA"
     gene            complement(1695..3161)
                     /gene="zwf"
                     /locus_tag="PKI23_01265"
     CDS             complement(1695..3161)
                     /gene="zwf"
                     /locus_tag="PKI23_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNN35599.1"
                     /translation="MTLTPLCPFEMVVFGGTGDLALRKLMPALYHRYQEGQFPADCRI
                     IAVSRTPLNREGYLVELRRALQKYVAGSHFEEHSFNSFAAFVDYVELDGSQPEGYQQL
                     RQRLHGDHDTPRVFYLATAPSLFGPICHQLRQAGLVHAGTRVVLEKPLGHDLASSRLI
                     NDQVGEVFGEQQIYRIDHYLGKETVQNLMVLRFGNALFEPIWRTGFIDHVQITVAEQV
                     GVEGRGGYYDQAGALRDMVQNHLLQLLCLVAMEPPSTLDANAVRDEKVKVLNALEPIT
                     AQNVTSQTVRGQYRAGAVGGVPVPGYLDESGIDPLSSTETFVAIKTQISNWRWAGVPF
                     YLRTGKRLQNRISEIVINFHQVPHSIFEPGSGALINNRLVLRLQPEEGVRLAMMAKQP
                     GPGMQLRSVDLNLDFAETFKGRLPDAYERLLIDVIRGRPTLFMRRDEQEAAWRWCEPI
                     LAGWDQHPDNLKKYISGTWGPTAAIALIERDGFTWHEE"
     gene            complement(3158..3355)
                     /locus_tag="PKI23_01270"
     CDS             complement(3158..3355)
                     /locus_tag="PKI23_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35600.1"
                     /translation="MTPSDADQPLKDKKKGHFTRLADSALYKKIEIPCHLGEFAPHMQ
                     REKNWYGCCVADGGCNLELSR"
     gene            3354..5186
                     /gene="edd"
                     /locus_tag="PKI23_01275"
     CDS             3354..5186
                     /gene="edd"
                     /locus_tag="PKI23_01275"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HNN35601.1"
                     /translation="MTRLHPRIASVTERIRQRSSGPRAAYLDRISAARGRGPRRRALS
                     CTNLAHGFAGCANAEKQLLRGAEGANLAIVSAYNEMLSAHQPFVDYPGWIKAAAIRAG
                     GSAQFAGGVPAMCDGVTQGQPGMELSLFSRDLIAMATAVALSHEMFDAALYLGVCDKI
                     VPGLLIGALSFGHLPALFVPAGPMPSGLSNSEKAAARQRHAEGKLSQIELLDVEARAY
                     HSPGTCTFYGTANSNQMLMEVMGLHLPGSAFINPGTPLRRALTEAAAQRAMEITALGP
                     HCTPVGEVIDERAIVNGIVGLLATGGSTNHCIHLVAIARAAGILIDWNDFDALSAVVP
                     LLARIYPNGAADVNHFQAAGGMGLLIRELLEGGLLHGDVRTVAGERGLAAYTEVPLLD
                     ETGQLIWRPAPAASGDPDVLRGVAQPFSASGGLRLVEGNLGRAIVKVSAVAPAHRRVL
                     APARIFDSQQALQQAFEAGNLSGDLVAVVRFQGPMANGMPELHGLTPILSLLQARGQQ
                     VALVTDGRMSGASGKVPAAIHVTPECTQGGALAKLREGDRVLLDCERGILMAQVADDE
                     WQKRPLATADLSANESGYGRELFAPFRQQVGSAEQGATLLAPIG"
     gene            complement(5222..5725)
                     /gene="def"
                     /locus_tag="PKI23_01280"
     CDS             complement(5222..5725)
                     /gene="def"
                     /locus_tag="PKI23_01280"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNN35602.1"
                     /translation="MTILQILEYPDPRLRTRAAPVLTFDQALRRLADDMLETMYDAPG
                     VGLAATQVNVHQQLVVIDVSETRNAPLVLVNPTVRPLSTELLEWEEGCLSVPGFHDTV
                     ARPRHILVKAQDIDGNSFELEPAGLLAVCIQHELDHLNGKLFVDHLSPLKRQRIRKKI
                     EKQQKRA"
     gene            5953..7014
                     /locus_tag="PKI23_01285"
     CDS             5953..7014
                     /locus_tag="PKI23_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNN35603.1"
                     /translation="MKKSIGQVRTTAALVLALLLATVGVAADEVTLKAGHPDRYVVKK
                     GDTLWAIAGMFLNKPWHWPRVWQANPQIKNPHLIYPGDQLRLVHVDGEPRLLLDRGGD
                     ALESDGVTRLSPQVRVQPLESAIETLPAELVGPYLIKGRIVSAQEVEDAARVIAGPEG
                     RLLMGAGDRFVARGLPESAVQAYAVFRSGERYVDPDSGELLGIELIEVGRAGYLSGKG
                     EVATLELDQSAQEVRLDDRLLPMVEERVNSSYLPSAPANAVDGKIIAVLGGVKKVGRY
                     SVVALNRGRSSGLEVGNTLAISKKPPAVRDRQRGDWVDLPAERVGLLMVFRTFDKMSY
                     ALVLESSQPGNVGDLVSSP"
     gene            7044..8207
                     /gene="dprA"
                     /locus_tag="PKI23_01290"
     CDS             7044..8207
                     /gene="dprA"
                     /locus_tag="PKI23_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNN35604.1"
                     /translation="MSQPLAVDEQRLWLALLHTPGLGPRRLLRLLQALGSPAAIIEAN
                     MDRLRALGLDGELLANLLRNREADPPWRTLVENDLSWLERDALHLLSWGSPAYPPLLA
                     QTATPPPLLFVRGDVALLQRPQLAVVGSRSPTPAGIEIAQMLARALAEAGLIITSGLA
                     LGIDGAAHRGALSVPDGQTIAVVGTGLDSCYPARHRQLCEQIAAQGAVVCEFPFGTPP
                     RADHFPRRNRVIAGLALGVLVVEAGLGSGSLITARHALEAGREIFAVPGSILNPLSRG
                     GHALLRDGARLVERAEDVLEELGLTLSGRAATAAGPGMAQPPGLLTPTERELLRCIDF
                     ESTSVDLLVERSGLAVPQLLGLLSQLELKGWIRAGLGGYLRDPALGADHWAQL"
     gene            8287..8844
                     /locus_tag="PKI23_01295"
     CDS             8287..8844
                     /locus_tag="PKI23_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNN35605.1"
                     /translation="MVSQWRLLRAARLLRQGGVLAYPTEAVWGLGCDPANRQAVERLL
                     AMKQRAVSQGLILIAGSMAQLEPLLRDLSPSQRDELEASWPGPVTWLIPHHGRVPHWI
                     SGHHATVAVRLTAHPVAAELCRTFGGMLVSTSANPHRRPPARDRLQLLRYFGTELDEI
                     LPGALGGAARPSQIRDLASGRRIRD"
     gene            8891..9811
                     /gene="hemF"
                     /locus_tag="PKI23_01300"
     CDS             8891..9811
                     /gene="hemF"
                     /locus_tag="PKI23_01300"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNN35606.1"
                     /translation="MSFGSDGIARVSDYLQGLQDSICTSFEQIDGVARFREESWQRPG
                     GGGGRTRVIAEGGLFEKGGVNFSHVHGGNLPPSATQHRPELAGRSFQALGVSLVMHPR
                     NPYLPTSHANVRLFVAEAPDAPPVWWFGGGYDLTPYYPFESDCRHWHNTAKAACDPFG
                     AQRYPRLKKWCDDYFFLPHRNEARGVGGLFFDDLNELGFEREFAFLRAVGDSYLAAYL
                     PILERRRLLEYGERERQFQLIRRGRYAEFNLVWDRGTQFGLQSGGRTESILMSLPPLV
                     RWEYDYQPPFGSEEARLTEEFLRPRDWATT"
     gene            9825..10637
                     /gene="aroE"
                     /locus_tag="PKI23_01305"
     CDS             9825..10637
                     /gene="aroE"
                     /locus_tag="PKI23_01305"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNN35607.1"
                     /translation="MKQFAVIGNPVAHSRSPQIHAAFAGQFGIELDYRRILTEAADFA
                     ERVTTFFAEGGHGLNVTLPFKEAACALADELGPQAEAAGAVNTLSLTGDGRLRGDNTD
                     GIGLVRDLTDNLGIPLLGQRLLVIGAGGAVRGILPALLAAAPGELLLVNRTRSRADAL
                     VERFAALGSLRSGDFAELATEQCDLLIQGSSAAFSGEGLALPASLVAGGSVYDLVYGD
                     KAAPFLDWAARCGARRVADGLGMLVEQAAESFRIWHGVRPQTAPVIAQLRQH"
     gene            10665..12719
                     /locus_tag="PKI23_01310"
     CDS             10665..12719
                     /locus_tag="PKI23_01310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNN35608.1"
                     /translation="MDNPLLQRHEFPPFSAIAPAQIGPAFEQILAENRQAIARLVSAP
                     DSDSWAGLIEPLDELADRIGQALAPISHLNAVVSDESLRQAYNALLPKLADYNAEYDQ
                     NPLLFAAYQRLAQSPAHADLDATQQRIIEHALRDFRLGGIDLPAAQQQRYRQISARLA
                     ELSSRFSDQLLDATDDWSLTLDSAERLRGVPELAIAAARERAQGKGEAGYRLTLEFPV
                     YQAVITHADDRDLRLEIYRAYHTRASEQSAQSPQRDNGPLIEEILALRAEQARMLGFA
                     SYAELSLATKMADSPERVEQFLRELAQRSRAKASADHAELARFAAADGIDRLEAWDVA
                     YYSEKLRESAFAISQEALRPYFPLPRVMAGLFELIDRLFAVRFTEQSQFDRWHPDARL
                     FALHSDDRLLGHLYLDLHARPRKRGGAWMGSLRSRFLRPQGALQRPLAAVVCNFDPPA
                     PGLPALLTHNEVLTLFHEFGHALHHLMTEVDHPAAAGINGVEWDAVELPSQLLENWCW
                     QSEALRLISGHYQTEEPLPDELLARLAAARHFQSGMQMVRQIEFALFDLSIHSAGHGL
                     SVAEVQAQLDQIRAEVAVVNPPAWNRFQNGFSHIFAGGYAAGYYSYKWAELLSADAFS
                     RFEQQGLFDRESGQAFAREILRRGGSRNALDGFIAFRGRPPQIESLLRHSGIGSESEA
                     NG"
     gene            12712..12975
                     /locus_tag="PKI23_01315"
     CDS             12712..12975
                     /locus_tag="PKI23_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HNN35609.1"
                     /translation="MVDQPPVRKRFIAGALCPQCQQIDTLQMWEEAGVPHRACVRCGH
                     QLAMTAESLTPVWPAGRLEQRAAPQIEGQPLIFFPKPPARNDD"
     gene            13331..14431
                     /gene="coxB"
                     /locus_tag="PKI23_01320"
     CDS             13331..14431
                     /gene="coxB"
                     /locus_tag="PKI23_01320"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNN35610.1"
                     /translation="MGLLLFSSIALAEEPLSKLNLRPGVTEVSQKAFDLHMIILWICV
                     AIGVVVFSIMFWSILMHRKSLGVKPADFHESTKIELLWTIIPVFILVAMAIPATTTLV
                     SMYDTKESDLDIFITGYQWKWKYEYMGQDVSFFSVLATPQNEIHNQEAKGEFYLLEVD
                     QPLVVPVGKKIRFLVTAKDVIHAWWVPDLAVKKDAVPGFVNEAWTRIDKPGVYRGQCA
                     ELCGKDHGFMPIVVVAKSQEDYDKWLSERKEVARKEAELKSKQWTLAELTARGEEVYG
                     KNCAACHQVNGAGAPPAFPPLKGSKIATGDRAEHIKIVVNGKPGTYMAAYGPQLSEVD
                     LAAVITYERNAWGNSTGDQVNPIDIVNFKNSK"
     gene            14444..16039
                     /gene="ctaD"
                     /locus_tag="PKI23_01325"
     CDS             14444..16039
                     /gene="ctaD"
                     /locus_tag="PKI23_01325"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNN35611.1"
                     /translation="MGTLNPAVEGHHDDHHHGPAKGVARWLFATNHKDIGSMYLWLSF
                     AMFLLGGAFALVIRAELFQPGLQLVQPEFFNQMTTLHGLVMVFGAVMPAFVGLANWMV
                     PLMIGAPDMALPRLNNFSFWILPFAFLTLASTLFMEGGAPNFGWTFYAPLSTTYAPPS
                     VTLFIFSVHMMGVSSILGAINIIATILNMRAPGMTLMKMPLFVWTWLITAFLLIAVMP
                     VLAGVVTMMLMDIHFGTSFFNAAGGGDPVLFQHVFWFFGHPEVYIMILPSFGVASQII
                     PVFARKKLFGYESMVYATASIAFLSFIVWGHHMFTVGMPLAGELYYMYATMLIAVPTG
                     VKVFNWAATMFRGAMTFETPMLFAIAFVVLFTIGGFTGLMMSIAPVDFQYHDTYFIVA
                     HFHYVLVPGAVFSIFAGAYFWLPKWTGHMYDERLGKLHFWLSFFFVNLLFFPMHFVGL
                     AGMPRRIPDYALQFADFNMMASVGAFGFGLSQLLFLFIVIKCIRGGAQATAKVWEGAD
                     EGLEWTVPSPAPFHTFSTPPVVK"
     gene            16073..16651
                     /locus_tag="PKI23_01330"
     CDS             16073..16651
                     /locus_tag="PKI23_01330"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNN35612.1"
                     /translation="MRTQKKQSRLLLTLTLVVVGMFAFGFLLVPLYDVICRWTGLNGK
                     TAGQYELSSAEAVQVDQSRLVTVQFVATNNDRMSWEFRPMVASIQLHPGEVKEVKFYA
                     RNPEAKAMTAQAIPSVSPGATAEYLHKVECFCFAQQHLMAGQAVEMPLRFFVDPKLPA
                     HVTKLTLSYTLFDVDTLAHGAEARAQQSESTN"
     gene            16693..17589
                     /locus_tag="PKI23_01335"
     CDS             16693..17589
                     /locus_tag="PKI23_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNN35613.1"
                     /translation="MAKTTETPLYYVPEQSKLPVLTALALFCMLIGGGNFITANSHQT
                     DSTFAFWWMLSGFAMMAVIFYAWFGQVIRENDSGLYSDQLNRSFVMGMSWFIFSEVMF
                     FAAFFGALFYVRTLVMPWLAGEGDKGVSHMLWPDFQAVWPMINMPSATGFSVPSQVVD
                     PWHLPLINTILLVSSSVTLTWAHHALKAEQRSKLNFWLFVTVALGVSFLVLQAYEYYE
                     AYHHYGLTLNSGIYGTTFFMLTGFHGAHVTIGTLMLIVMLLRALKGHFKPNDHFGFEA
                     AAWYWHFVDVVWVGLFIFVYLW"
     gene            complement(17639..17833)
                     /locus_tag="PKI23_01340"
     CDS             complement(17639..17833)
                     /locus_tag="PKI23_01340"
                     /inference="COORDINATES: protein motif:HMM:NF022587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HNN35614.1"
                     /translation="MLLFCAILASLSSGFYFFIKDRGDTQSRRLLNSLAIRVSLAVLL
                     LLCVIYGLWSGELALRAPWQ"
     gene            17814..18713
                     /locus_tag="PKI23_01345"
     CDS             17814..18713
                     /locus_tag="PKI23_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNN35615.1"
                     /translation="MAQKSSIMVVLSHISEHPEGWSGSLNHESRVETREGPYAGSKGE
                     VPEWAGSLISKEKRQRFFRFKPNLKLTALALVMFPLLCALGFWQLGRMAEKQKILEQL
                     ELQQRLSPVSMEELPDQLEQLRGRRVKITGEFDNDRVILLDNRIMAGHAGYEVLVLFR
                     TASGARNLWLNRGWVAADGNRQRLPEIAPVVGQVTVVGEIYVPYGAAILLGANQAEGR
                     DWPKVVQAAELSMLRVLWGVELYPHQIRLLPEQPGVLQRPWTTVNVTPEKHLGYAVQW
                     FALAAALLLLYLYSSCVVEEEAA"
     gene            18710..19357
                     /locus_tag="PKI23_01350"
     CDS             18710..19357
                     /locus_tag="PKI23_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35616.1"
                     /translation="MKSSQPAKNVMNAQSQPQKRPWVLYAIVLGTALPVLAAYLFHYY
                     ASVWQPGSQTVNRGVLLSPPLNIQNFKLLDRAGQPFFKEEFPGKWLILQIASDECGEP
                     CQVRRHELRQVHVALGKEMTRVNRLLVQGREDRNFVDDQQTKENEPPLRVAYGDLLEI
                     RHHLAQIDALRSVEVSELVFLVDPLGNIMMYYTPTHSGKDLLKDLQKLLKASQIG"
     gene            19365..20390
                     /locus_tag="PKI23_01355"
     CDS             19365..20390
                     /locus_tag="PKI23_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNN35617.1"
                     /translation="MVTGFKRKPGFMLALCSTFFTLIVVLLGAYTRLSDAGLGCPDWP
                     GCYGFVSVPRSEAQIDLAESRYPHAPVEAHKAWPEMVHRYFAGTLGLLVLANLIVAWR
                     HRHLPQQPLGLPLALAVLVVVQALFGMWTVTLKLWPQVVTAHLLGGFATLTLLWLLTL
                     RLSNWGDSAAAISTRSAGMRRLTRLGLLLLLLQIALGGWLSSNYAALACPDLPTCQAQ
                     WWPEMDFRAGFNLLQDVGPSYLGGLLHNSARMAVHVTHRLGALVVTAILLLLAFNLFR
                     HSGVARKLAGLLLLVLTLQLSLGVGNILLQLPLPIAVAHNGVAALLLLTLTAIDHFLS
                     RRAAAGR"
     gene            20411..21310
                     /gene="cyoE"
                     /locus_tag="PKI23_01360"
     CDS             20411..21310
                     /gene="cyoE"
                     /locus_tag="PKI23_01360"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNN35618.1"
                     /translation="MEQAGKPIGLLDQFNDYLELCKPKVVALMILTSIVGMFLAVPGM
                     VPWDVLLLGNLGIALVSASGAVINHVIDRRIDTLMARTHKRPVAVGRVSPARAMLFAL
                     LIGVVGMAILLIFVNPLCAWLTLAAFVGYAFIYTGYLKRATPQNIVIGGIAGAAPPLL
                     GWSAVTGTVDPHALLLVLIIFAWTPPHFWALAIHRKEDYAKANIPMLPVTHGDRYTKL
                     HILLYTAVLFAAALLPAATGMSGWIYLAGASLLGAGFIYRAVQLCLDEKSGAPMATFR
                     YSILYLMALFVVLLVDHYFLIRL"
     gene            21307..21963
                     /locus_tag="PKI23_01365"
     CDS             21307..21963
                     /locus_tag="PKI23_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNN35619.1"
                     /translation="MSGGATRGKWITVAILTVVVTAIGLVFSSNVTKPRALSVEQLRT
                     NGLYLLEQPKSLGEFALTTATGTAFTAAELRGHWTLLFMGFTHCPVFCPTTLATLNEL
                     VTGKLGETPYAADTRVVMVSLDPQRDSAEKLAGYLNGFNPGFTGVTGSFEQIFQFSQS
                     LGFSFAKLPLANGDYTIDHAGMIALIDADGHYRGYFKPPHDAAQMLVTYRSARAGLLR
                     "
     gene            complement(21972..22745)
                     /locus_tag="PKI23_01370"
     CDS             complement(21972..22745)
                     /locus_tag="PKI23_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNN35620.1"
                     /translation="MTSAYIFSGFVVGAIVGVTGVGGGSLMTPLLMLLFGVHPATAVG
                     TDLLYAAITKMGGTTVHARKGHVDWKITGRLAAGSIPATILTLSALSYFGLPEKHLSV
                     VISSVLGVALLLTAVALIFKQRIQAFALSHHSDHWRTRWLGPVTVATGALLGVLVSLS
                     SVGAGAIGVAVLFFLYPQLSAIRIVGSDLAHAVPLTLVAGIGHWMMGSVDWSLLGSLL
                     IGSLPGIYLGSHLAARMPERLLRPVLASMLVLIGVKLLV"
     gene            complement(22965..23243)
                     /locus_tag="PKI23_01375"
     CDS             complement(22965..23243)
                     /locus_tag="PKI23_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNN35621.1"
                     /translation="MRSLRLLSILFALCLALPAFTEEAAPGEAEVIIHEGKDKTIHEY
                     RINGFTYAVKVIPKNGKPYFLVTTNGKDFQNIDQPNMLIPSWEIFTWK"
     gene            23333..26038
                     /gene="polA"
                     /locus_tag="PKI23_01380"
     CDS             23333..26038
                     /gene="polA"
                     /locus_tag="PKI23_01380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNN35622.1"
                     /translation="MSETQNRPRQLVLVDGSSYLYRAYYALPPLSTRDGAPTGAVKGV
                     ISMVRKLLKEYPDATVVVVFDAKGKTFRDELFAQYKSHRPPMPTDLAQQIEPIQQIIR
                     ALGLPLLVVEGVEADDVIGTLARQATEMQIPTVISTGDKDMAQLVNGHVTLINTMTDT
                     RLDRAGVEQKFGLPPELIRDYLALVGDSSDNIPGVPQVGPKTAVKWLSSHGDLDGLLA
                     QAAEIPGKVGESLRLHLDQLALSRQLATIRCDLELPIDLATLQYGEVDRARLVELFAQ
                     LEFNSWLDEYRDKQPASASHLPLRHETLLTAEQFERWLQKLTAAANFVIDTETSSLDC
                     ISAQLVGLAFAVEPGEAAYLPLRHDYPDVPPQLDCDWVLSRLRPLLEDPGHGKIGQNL
                     KFDLGILARHGIALRGIAEDTMLESYVLNSTASRHDMDSLALRYLGRQTISFEQIAGK
                     GSKQLSFNQIALEQAAPYAAEDAEVPLRLHQVLGPQLDAEPRLRQVYRELELPLVPIL
                     SRIERAGVLIDAERLTRHSGELAVRIAELEQQAWQLAGATFNLGSPKQLQEIFYERLQ
                     LPVLQRTPTGQPSTAEAVLQELALDYPLPKVLLEHRSLSKLKSTYTDRLPEQIHPLTG
                     RVHTSYHQAVTATGRLSSSDPNLQNIPIRTSEGRRIRQAIIAPAGHQLLAADYSQIEL
                     RIMAHLSGDAGLLNAFGRGLDVHRATAAEVQGIPYEQVTSEERRRAKAVNFGLIYGMS
                     AFGLARQLGISRNEAQGYIDGYFSRYPGVLDYMERIRQTAREQGYVETLHGRRLHVPE
                     IHARDQARRRAAERTAINAPMQGSAADIIKLAMIQLDEWLERTRAPARMIMQVHDELV
                     LEVANEALEQVRPEVKRIMEQVTTLAVPLLVEIGTGPDWDVAAQR"
     gene            26243..28597
                     /locus_tag="PKI23_01385"
     CDS             26243..28597
                     /locus_tag="PKI23_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN35623.1"
                     /translation="MVLQDTTLPSGGVDSTALTAKVHGEQVRLLYASLPLSLLINVVN
                     ASLLAFILWGAIDTTRLLNWLVSIQLVTLGRGLLLLGYRRMASDVTRPKVWIRGFRIG
                     VVAVGLCWGVAGWWLFIPDDVAHQAFLALVLAGMSAGALSAMSADRPSVLAFMLLSLL
                     PLLVQLMVAQASIQLVMSVMVLLFLASLISNALRIHHTIVENLCLRMEAEPRERMLRQ
                     QEERYRALIDTLQEGFQILSHDWRRLYINDAAARQLRRSRQVLLGELPEQWKKSAFFA
                     LLEQCMEQRQPLHVEQKITLHDGSQGWFELSIQPVEEGISVLSIDITARKEAEEEINR
                     LAFHDSLTRLPNRRLLFDRLQQALRSSVHGQRPGALLFIDLDDFKTINDTLGHGVGDL
                     LLREVANRLAHCVRQADTVARQGGDEFVVLLEALGPTDEEAIAQAERVAEKIRESLLQ
                     PYQLAGHHGRHCTASIGIALFMDAQASADQLFKQADIAMYQAKQAGRNAIRFFDPQMQ
                     ASIEQRSRLETELRQAVQQRQFVLHYQVQVDHDGQPLGAEILLRWHHPERGLIAPAEF
                     IPLAEETGLIIPIGEWLLEEACWQLKKWEREPDRAQLHLAINVSARQFRQPDFVERVV
                     GMLQQTGIDPRKLKLELTESLVLDNVAEAIATMQRLKESGVQFSMDDFGTGYSSLAYL
                     KKLPLDQLKIDQSFVSEIDTDPDDATIVQTIVAMAKNLDMEVIAEGVESELQRNFLIE
                     CGCLNFQGYLFGKPLPLAEFELLLQELAGRRKQVTPIRIGAGAQ"
     gene            28746..29747
                     /locus_tag="PKI23_01390"
     CDS             28746..29747
                     /locus_tag="PKI23_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNN35624.1"
                     /translation="MPESVRKPIDTALQHDLHALARDEFALVNQTILRQLHSDVELVR
                     EIGHYLIESGGKRLRPLVVLLAAGACQYRGSAHIDLAVVVEFLHTATLLHDDVVDMSQ
                     LRRGRTTVNARWGNAPSVLVGDFIYSRAFELMVKIGSLQIMQVLAETTNKLAEGEVMQ
                     LLNAGNPDVDETRYREVILRKTAKLFEASAQSAAILAGRPATEVEALASYGRHLGLAF
                     QLIDDVLDFDGNSDLLGKNLGDDLAEGKPTLPLIITLERGAPADAALVRDTILNGSSN
                     ALNAVIDAVKRSGGLEYTRELARHEAESAIESLHVLAPSRHRDGLIRLARFAVERQY"
     gene            29768..29844
                     /locus_tag="PKI23_01395"
     tRNA            29768..29844
                     /locus_tag="PKI23_01395"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29802..29804,aa:Pro,seq:cgg)
     gene            29930..30544
                     /gene="gmk"
                     /locus_tag="PKI23_01400"
     CDS             29930..30544
                     /gene="gmk"
                     /locus_tag="PKI23_01400"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNN35625.1"
                     /translation="MPTGQLYIISAPSGAGKTSLVKALLEQDSDLAVSVSHTTRPRRV
                     GEVDGVNYHFITTQQFLDLLAQDAFLEHAEVFGHYYGTSHLAVARQLERGQDVILEID
                     WQGARQVRRLIDDAVSIFILPPSRAELERRLTARGQDGVEVISARMAKATAEMSHYPE
                     FDYLLINDSFERALTELQAIVLAKRLELPRQSDKARNLIAELLS"
     gene            30611..30859
                     /gene="rpoZ"
                     /locus_tag="PKI23_01405"
     CDS             30611..30859
                     /gene="rpoZ"
                     /locus_tag="PKI23_01405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNN35626.1"
                     /translation="MARITVEDCLPHVDNRFDLVMLASKRARQLAVQGREPLVPWEND
                     KPTVVALREIAAGLIDRDYVNRLEREDEQHSVLFDAMP"
     gene            30890..33082
                     /locus_tag="PKI23_01410"
     CDS             30890..33082
                     /locus_tag="PKI23_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016712710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNN35627.1"
                     /translation="MNGAPTITATAVDSPTDGREAGPVSPGLARLIATLADYLDAARI
                     DEIIRAYHYAAAAHGCQTRRSGEPYITHPVAVGQILAEMHMDHHGIMAALLHDVLEDT
                     SADEQELRTLFGDTVAELVDGVSKLQRMNFASQAEAQAENFLKMTMAMAKDIRVILVK
                     LADRLHNMRTLGVLASAKRRRIARETLEIYAPIAHRLGMYEIRAELEDLGFQALYPMR
                     ARRIEAAVRKARGNRDEMLATLRQAIQARLEQTGLPCRVMGRERHLYSIYRKMREERK
                     SFNKIMDVYAFRVIVDQVDSCYRALGVLHGLYKPVPGRFRDYIAIPKGNGYQSLHTTL
                     IGLRGVPIDIQICTEQMEAMAHHGVAARRFLDGCSESPSSGEDRTNQWLQGLLEMQKV
                     AGDSIEFIENVKIDLFPDEVYVFTPKGKILELPVGATAIDFAYAIHTDIGHSCVGCQI
                     DQRLAPLSEQLRSGQTVKIITSPGARPNVSWLNFAVTAKARSSVRHYLKNQQYTEAVD
                     LGRRLLQKSLSVCQKSLDELPQAQIDQLLQEYRLNGLDALLSEIGLGNRPVQEIVRKL
                     LPGVSGTLQLGMVDSGTNALIIGEKGGMLLNYARCCYPIPGDAVVGQLSSGRGITIHQ
                     ETCRHVEPILGDSLHCLAVQWQSGIEGEFVAGLRVEMKNKRGMLASVASAIADSDSNI
                     ERVTTADRHGDISMLVIEVSVRDRVHLARVIRKIKAIDAVSRVFRSRS"
     gene            33101..33484
                     /locus_tag="PKI23_01415"
     CDS             33101..33484
                     /locus_tag="PKI23_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNN35628.1"
                     /translation="MNRRIIRTDQAPQAIGTYSQAVEAQGTLYVSGQIALDPVTMVLV
                     NDSIENEITQVFNNLQAVVAAAGMTLDEAVKLNIYLTDLIHFATLNQIMARFFNEPYP
                     ARAAVGVAQLPRGARVEVDAVVVRR"
     gene            complement(33531..34010)
                     /gene="ssb"
                     /locus_tag="PKI23_01420"
     CDS             complement(33531..34010)
                     /gene="ssb"
                     /locus_tag="PKI23_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNN35629.1"
                     /translation="MARGINKVILIGNLGADPEVRSTASGTQITSIRLATSDSWTDKQ
                     GQRQERTEWHRVVFFNRLAEIASQYLRKGSKVYIEGSLRTQQWEKNGEKRYSTEIHAN
                     EMQMLDGRAGGGQESYGGDYGEGPPMSPRQSSGGGSRANADADMGGRFDDFDDDIPF"
     gene            complement(34046..35419)
                     /locus_tag="PKI23_01425"
     CDS             complement(34046..35419)
                     /locus_tag="PKI23_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN35630.1"
                     /translation="MNSAELRATLALSLLFALRMVGLFMVLPVLTLHQSELRDATPLL
                     MGLALGAHGLTQALLQIPFGLLSDRIGRRPVISLGLLLFIAGSVVAALSSSVYGIIAG
                     RLLQGSGAIASPLMALLADLTRDQVRTQSMAIMGVSIGLSFSAALILGPMVTHVSGLS
                     GIFWISALLAIAGELILLLLVPKARQQIIHRNVEAVPGQLGQVLADRELLRLNFGVFA
                     LHFMMMASFVVLPMILTQQLSIPVGQHWHYYLPIIGVSFFAMLPFLIIAETRQQMKPV
                     FLGALALVIISQLLLSFDQSDLWSVVAALFVFFMGFNLLESTLPSLVSKMAPAGSKGT
                     AMGMSSSSQFLGAFFGAASGGALMHWCSASRGYQPLFLALAGIGLLWFLLACGMKRPR
                     TLLDLRLDEPAPEDPYQAELLLHELRALAGVAEVALFPDSGEIYLKLDRQRLDDHARH
                     RLSLLDA"
     gene            complement(35565..35882)
                     /locus_tag="PKI23_01430"
     CDS             complement(35565..35882)
                     /locus_tag="PKI23_01430"
                     /inference="COORDINATES: protein motif:HMM:NF038106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HNN35631.1"
                     /translation="MTTTPPKRKDKKAVIGQSWSDEHLRSLLDLQPPEGLSADHHRLL
                     QAYRSMIAEDFSRFIGHFIAAGHDLDATDPRGQSVARIIAGHPKGEEYLIALRQAGAR
                     MTD"
     gene            complement(35936..36646)
                     /locus_tag="PKI23_01435"
     CDS             complement(35936..36646)
                     /locus_tag="PKI23_01435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNN35632.1"
                     /translation="MINEHRPYYIKRLHMAVQSFYTRHFVAPQLTHLGHGHAILKPWH
                     LLIYGQPISIGDFAHIICTPNAQVQLTVLGKGRIEIGKHVLITPGVRMSSASMIQIGD
                     DCMFAYNAYLTDADWHGIYHREKAIGATAPIVLGNNVWVGDSAIICKGVTIGDNSIIG
                     AGAVVAKDIPPNSIAVGNPARVVKELDPQIPITTRAELFRNPEQLQRELEHIDRNLLK
                     NNSLLGWLRSKLLPRPGD"
     gene            complement(36832..37275)
                     /locus_tag="PKI23_01440"
     CDS             complement(36832..37275)
                     /locus_tag="PKI23_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNN35633.1"
                     /translation="MMALVKWEPLREIEEMFDRYSKAMGWPRANIGQELVSGDWLPKV
                     DIAETDQAFEIKVELPEVNREDIKIGVENGVLSIRGERKQEKEEKGKKFHRIERHYGS
                     FVRSFSLPENVDPDKVKATFKDGMLNLHLEKSQKSTPKAIDVKVE"
     gene            complement(37401..38414)
                     /locus_tag="PKI23_01445"
     CDS             complement(37401..38414)
                     /locus_tag="PKI23_01445"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNN35634.1"
                     /translation="MKRGESHRRRRGAGHFLLLGLLLCGTLASASEAPLDRIAFGSCN
                     RSDQAQPIWPAVLQLRPQLWIWSGDIIYADTEEMDSMAKLYRQQKSQPDYQKLLACCQ
                     VIGTWDDHDYGRNDGDRSYRQKVESQRLLLDFLDEPANSPRRRQAGIYASHLQGPPGQ
                     RVKVILLDTRFQREPPGRMADILGAEQWQWLARELDPGSAELFIVVSSIQVIPVEHRH
                     EKWANHPAARTRLLQLLADARLPALVLLSGDRHFAEIMRLDLDRLPYPLFEITSSGLT
                     HSWENFPGEPNRYRIGEPYTGLNHGVLLIDWQDNQPRVEVQIRDRDGHPQLRQPIQFN
                     RNP"
     gene            complement(38401..38889)
                     /locus_tag="PKI23_01450"
     CDS             complement(38401..38889)
                     /locus_tag="PKI23_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNN35635.1"
                     /translation="MAIEIERKFLVNAALWQPQGQASHYRQGYLCVADDRVLRVRIAG
                     QSAWLTLKGSLPGQSIARLEYEYPIPLTDAQEMLDRLCLWPPIDKVRHRLQHGQHLWE
                     VDIFAGANSGLMLAEVELEHVDELVELPPWVGREVSDDPRYSNACLSQHPYSLWTAHE
                     KG"
     gene            complement(39023..39097)
                     /locus_tag="PKI23_01455"
     tRNA            complement(39023..39097)
                     /locus_tag="PKI23_01455"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39062..39064),aa:Glu,seq:ttc)
     gene            complement(39117..39192)
                     /locus_tag="PKI23_01460"
     tRNA            complement(39117..39192)
                     /locus_tag="PKI23_01460"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39157..39159),aa:Ala,seq:ggc)
     gene            39369..40043
                     /gene="radC"
                     /locus_tag="PKI23_01465"
     CDS             39369..40043
                     /gene="radC"
                     /locus_tag="PKI23_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNN35636.1"
                     /translation="MSIKHWPAAERPREKLLMRGAAALSDAELLAIFLRTGVRGKSAV
                     DLARELLVEFGGLRPLLEADLQRFCRGSGLGEAKYVQLQAVLEMSSRHLQQGLERGDA
                     LTSPAQTRRFLSSRLRHLEREVFMVLYLDNRHRMIAFEELFKGSINSAEVHPRELIKR
                     VLHFNAAAVILAHNHPSGIAEPSQADREITQRLRAALALVDVRLLDHMVVGDTEVISL
                     AELGCL"
     gene            complement(40070..40516)
                     /locus_tag="PKI23_01470"
     CDS             complement(40070..40516)
                     /locus_tag="PKI23_01470"
                     /inference="COORDINATES: protein motif:HMM:NF023982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA sensor PanZ family protein"
                     /protein_id="HNN35637.1"
                     /translation="MPVLLEQLVTPSAQDVADLIKIYHDDPGFAGQDEARIQRFVEER
                     LQTHSLYGGRFNGRLLAAMVVDRQSTPWRMELLCVRSLTRRRGVARRMMELIEAEAAT
                     AGQGLIATIPTGDATLLQLARKAGFHVKQVMPPATIEIEWSPLRIA"
BASE COUNT         7309 a        12004 c        13278 g         8106 t
ORIGIN      
        1 acctgctggt gcgtgcgctc tcgatgggca aactgcacca cgccatgatg ggcacctgcg
       61 cggtcgccat tggcaccgcg gcagccattc ccggcacact ggtcaacctg gccgccggtg
      121 gcggcgagcg cgaggcggtg cgcttcggcc atccgtcggg caccctgcgc gtgggtgccg
      181 aggcgcggca ggaaaacggc gagtgggtgg cgaagaaggc gatcatgagc cgcagcgccc
      241 gcatcatcat ggaaggctgg gtaagggtgc cggaaggaag cttctgagct gcaagcgact
      301 acccggcagt ccactggtcg atggtgatgt ggccgggttg cgcccggatc cgttgcagcc
      361 acgccagcac ggccggatag cgtgacagat cgaagccgcc ctcatgggcc tcgtgggtgt
      421 aggcatagag ggcgatgtcg gcgatggtgt aacgctcagc gaccatgaac tgacggtgct
      481 gcagatgctc atccatcacc gccaatgcct gatttccggc cttttgcttc tgtgccagca
      541 gctcaaggtt gaagggtgtc ggttcgaacc ccttgatcac aagccagaac cttggcgtgg
      601 cgatgttcgg ctcatggctg tactgctcga agaagagcca ctgcatcacc tgcgcctgtt
      661 cgaggcgctc cgtcggccag aagggtgtgc cttgggacag aaaacagaga atggcattgg
      721 attccggcag gcagctgcca tcctccagct caagcagcgg aatgcggccg ttgcggttct
      781 ttttcaggaa cgccggcgtt ctggactccc cccgcagaat gtcgatcggc aggtactggt
      841 aaggctgtga cagttgcgcc agcaacagtc gcaccttgta gccgttgccc gatctggggt
      901 cgtcgtagag cttcacgctg cgcctcattg cattcgtcgc tgtaaaaagc cctcttcggc
      961 ggtgatgatt gcgatccacc ctgcaggcgg cctcaggcac tccagtacag atcgagcggc
     1021 accctctgct gctccagcac tgcccgcacc gggcaggttt cgaccgcgcc gggttgtcgc
     1081 gcctgcaggt agcgttgcca cttctgttcg ccctcgatgt gcagatagag ctgccggctg
     1141 tcgagcaggg tggcgagggt caggctgacg cgcggatggt cgacgccggc cggtggcatg
     1201 gcggcaaagg tggcgctgtg ctgttcatgc agaccggttt gcagctgcgg actgcctgga
     1261 aagagcgagg cggtgtggcc atccgtgccc atgccgagaa tcaccgcatc gaacgggcga
     1321 tgcagttggg cgaacagccg ttcgctttcg gcatggccga gccaggggtc ggcagcggcg
     1381 gtcaccagtg gcacgaaccg cgccgccgcg gcggctgcgc gcagaagttc gcgcctgacc
     1441 agcccggcat tgctgtccgg atgggtttct ggcacccagc ggtcgtcggt caggctgacg
     1501 atgatgcgtg accagggcag tggctgctgg ctcagtcgct gaaagagcgc tgtcggcgta
     1561 cgtccaccgc tgaccagcag tgtcgccgcc gcacgctggt cgagggcctg ttgcaggtcg
     1621 gcgacgatgg actgggtcag tgccgcgacc agttgtgtcg agtcggcgaa acggtgctcg
     1681 cgaaccacgt cggctcactc ctcgtgccag gtgaagccat cgcgctcgat cagtgcgatg
     1741 gcggcggtgg gtccccaggt gccggagatg tacttcttga ggttgtctgg gtgctggtcc
     1801 cagccggcga ggatcggctc acaccagcgc caggcggcct cctgctcatc gcggcgcatg
     1861 aagagcgtcg gtcgaccgcg aatcacatcg atcagcagcc gttcgtaggc atcgggcagc
     1921 cgccccttga aggtttcggc gaagtcgagg ttgagatcga ccgagcgcag ttgcatgccc
     1981 ggtccgggct gcttggccat catcgccagc cggaccccct cctcgggctg caatcgcagc
     2041 accagccggt tgttgatcag cgcgccggag ccgggctcga agatcgagtg gggcacctgg
     2101 tgaaaattga tgacgatctc ggagatgcgg ttctgcagcc gcttgccggt gcgcaggtaa
     2161 aacggcacgc cggcccagcg ccagttggag atctgggtct tgatggcgac gaaggtctcg
     2221 gtgctgctga gcggatcgat gcccgactcg tcgaggtagc cgggcaccgg tacgccgccg
     2281 accgcgccgg cacggtactg tccacgtacg gtctggctgg tcacgttctg cgcggtgatc
     2341 ggttccagtg cattgagtac cttgactttc tcgtcgcgga ccgcattggc atcgagggtc
     2401 gaggggggct ccatcgccac caggcagagc agttgcagca ggtggttctg caccatgtcg
     2461 cgcagtgcac cggcctggtc gtagtagccg ccgcgtcctt cgacccccac ctgctcggcc
     2521 acggtgatct gcacatggtc gatgaaacca gtgcgccaga tcggttcgaa cagggcattg
     2581 ccaaagcgca gcaccatcag gttctgcacg gtctccttgc ccaggtagtg gtcgatgcgg
     2641 tagatctgct gctcgccgaa gacttcaccc acctggtcgt tgatcagccg cgaggaggcc
     2701 aggtcatgac ccagcggttt ttccagcacc acccgggtgc cggcatgcac cagtccggcc
     2761 tgtcgcagtt ggtggcagat cggtccaaac agcgacgggg ccgtggccag atagaaaacg
     2821 cgtggggtgt cgtggtcgcc atgcagccgc tgacgcagct gctgatagcc ctcgggctgg
     2881 ctgccgtcga gttcgacata gtcgacaaag gcggcgaagc tgttgaagct gtgctcctcg
     2941 aagtgggagc ccgccacata tttctgcagg gcgcgccgca gttcgaccag atagccttcg
     3001 cggttcagcg gggtgcgcga gaccgcgatg atgcggcagt cggccgggaa ttgcccctcc
     3061 tgatagcggt ggtagagtgc cggcatcagc ttgcgcaggg cgaggtcgcc ggtgccaccg
     3121 aagacgacca tctcgaacgg gcagagcggg gtcagggtca tcgcgacagc tccaggttgc
     3181 aaccgccatc agccacgcag caaccatacc agtttttctc ccgctgcatg tgcggcgcga
     3241 actcaccaag gtggcatgga atttctatct tcttataaag ggcactgtca gccaggcggg
     3301 tgaagtggcc ctttttcttg tctttaagtg gctgatctgc atcggagggc gtcatgacca
     3361 ggcttcatcc ccgcatcgca tcggtgaccg agcgcattcg ccagcgcagc agtggcccgc
     3421 gcgccgccta tctcgatcgc atcagcgcgg cccgagggcg cggaccacgt cggcgggcgc
     3481 tctcctgcac caacctggcc catggtttcg ccggctgcgc caatgccgaa aagcagttgc
     3541 tgcgcggggc tgagggcgcc aacctcgcca tcgtctcggc ctacaacgag atgctgtcgg
     3601 cgcatcaacc cttcgtcgac tatcccggct ggatcaaggc ggccgcgatc cgcgccggcg
     3661 gcagtgccca gtttgccggt ggggtgccgg cgatgtgcga tggtgtcacc caggggcagc
     3721 caggcatgga gctgtcactg ttcagccgcg acctgatcgc gatggccacc gcggtggcgc
     3781 tctcgcacga gatgttcgat gcggcactct atctgggcgt ctgcgacaag atcgtgccgg
     3841 ggctgctgat cggtgcgctc agctttggcc acctgccggc gctcttcgtg ccggcaggtc
     3901 cgatgccatc cgggctctcc aacagcgaga aggcggccgc gcggcaacgc catgccgagg
     3961 gcaagctgag ccagatcgaa ctgctcgatg tcgaggcgcg cgcctaccac agtcccggca
     4021 cctgtacctt ttatggcacg gccaactcca accagatgtt gatggaagtg atgggactgc
     4081 atctgccggg atcggccttc atcaaccctg gcacgccact gcggcgcgcc ttgaccgagg
     4141 cggcagcaca acgggccatg gagatcaccg cactgggacc gcactgcaca ccggtggggg
     4201 aggtgatcga tgagcgggcc atcgtcaatg gcatcgtcgg cctgctggcc accggcggtt
     4261 cgaccaacca ctgcatccac ctagtggcca tcgcccgggc cgccggcatt ctgatcgact
     4321 ggaacgattt cgacgcactg tcggcggtgg tgccactgct ggcgcgcatc taccccaatg
     4381 gcgcggccga tgtgaaccac tttcaggccg ctggtggcat gggactgctg attcgtgaac
     4441 tgctggaagg tggtctgctg catggcgatg tccgcaccgt cgccggggag cgcggtctgg
     4501 ccgcctacac cgaggtgccg ttgctcgatg agactggcca gctcatctgg cggccggcgc
     4561 cggcagcgag tggtgaccct gatgtgctgc gtggtgttgc gcagcccttc tccgccagcg
     4621 gcggtttgcg gctggtcgag ggcaacctcg ggcgcgccat cgtcaaggtg tcggcggtgg
     4681 cgccggcaca tcggcgggtg ttggcgccgg cgcggatctt cgactcccag caggcgctgc
     4741 agcaggcgtt cgaggccggc aatctcagcg gtgatctggt agcggtggtg cggttccagg
     4801 gaccgatggc caatggcatg ccggagctgc atggcctcac accgatcctg agcctgctgc
     4861 aggcgcgcgg acagcaggtg gcgctggtga ccgatggccg catgtcgggc gcctcgggca
     4921 aggtgccggc ggcgatccat gtcacacccg agtgcacgca gggtggtgcg ctggcgaaac
     4981 tgcgcgaggg tgacagggtg ctgctcgatt gcgagcgtgg catcctgatg gcgcaggtgg
     5041 ctgacgatga gtggcagaag cggccgctgg cgacggccga cctgagcgcg aacgagtcag
     5101 gatacgggcg ggagctcttc gcgccgttca gacagcaggt gggctcggcg gagcagggtg
     5161 cgacgctgct ggcgccgatc ggttgaagtt cagcgcggcg gttccatcct gaaagccccg
     5221 atcaggcccg cttctgctgt ttttcgatct tcttgcggat gcgctggcgc ttcagaggcg
     5281 agaggtggtc gacgaagagc ttgccgttga gatggtccag ttcatgctga atgcagaccg
     5341 ccagcaatcc cgcaggttcg agttcgaatg agttgccgtc gatgtcctgt gccttcacca
     5401 gaatatgcct gggacgtgcg accgtgtcat gaaagccggg cactgacagg cagccctcct
     5461 cccactccag cagttcggtc gaaagcggcc gcaccgtggg gttgaccagc accaagggcg
     5521 cgttgcgcgt ttcgctgacg tcgatcacca ccagttgctg gtgcacattg acctgggtgg
     5581 ccgccaagcc gacacccggg gcgtcataca tggtttcgag catgtcgtcg gccagacggc
     5641 gcaatgcctg atcgaatgtc aaaaccgggg ccgcgcgggt gcgcagtctg gggtctggat
     5701 attcgaggat ctggaggatg gtcatgctgg tgcgcctgcg ctctggcgga tttcgaaagg
     5761 gtactgctaa gattatcgta tccagcagcg cgcccacaag gcacgctgcc gttcaccagg
     5821 cagcgcctgc cggctcggcc ggctctgctt gagatgaggt ggttgaccgg tttttcaatc
     5881 cccctttcat gctgccgaat ggcggttccg gtgcacgaga gttcgcatcg gccacactgt
     5941 gagtacagaa caatgaagaa gagtatcggg caggtccgga ccaccgccgc gctggtgttg
     6001 gcactgctgt tggcgacggt tggcgtggct gccgatgagg tgacgttgaa ggcgggccat
     6061 cccgaccgct atgtggtgaa gaagggcgat acgctctggg ccattgccgg catgtttctg
     6121 aacaagccct ggcactggcc gcgggtgtgg caggccaacc cgcagatcaa gaacccccat
     6181 ctgatctatc ccggcgatca gcttcggctg gtccatgtcg atggtgagcc ccgtctgctg
     6241 ctcgaccggg gcggcgacgc tcttgagtcg gatggtgtga cccgcctgtc gccacaggtg
     6301 cgggtacaac cgctggagag tgcgatcgag acgctgccgg ccgagctggt gggcccctat
     6361 ctgatcaagg ggcgcatcgt cagcgcgcag gaggttgaag acgctgcgcg ggtgatcgcc
     6421 gggccggaag ggcggctgct gatgggggcc ggtgaccgct tcgtggccag aggactgcct
     6481 gaatcggcgg tccaggccta tgcggtgttt cgcagtggcg agcgctatgt cgatccggac
     6541 agcggtgagt tgctgggcat cgaactgatc gaagtggggc gtgccggtta cctctccggc
     6601 aagggggagg tggccacgct cgaactcgat cagtcggcac aagaggtacg gctcgatgat
     6661 cggctgctgc cgatggtcga ggagcgggtg aactccagct atcttcccag tgcgccggcc
     6721 aatgcggtcg atggcaagat catcgccgtg ctgggtggag tgaagaaggt gggccgttac
     6781 agtgtggtgg cgctcaatcg cggtcggtcg tcgggtctgg aggtgggcaa cacgctcgcc
     6841 atcagcaaga aaccgccggc ggtgcgtgac cggcagcgtg gtgactgggt cgatctgccg
     6901 gccgagcggg tggggctgct gatggtgttt cgcaccttcg acaagatgag ttatgccttg
     6961 gtgctcgaat cctcgcaacc gggcaatgtc ggtgatctgg tctcctcccc ctagacagct
     7021 ctcgccgtca acctgaaacc gcgatgagcc agccgctggc ggtcgatgag cagcggctct
     7081 ggctcgctct gctgcacacg cccggcctgg gtccgcgccg gctgctgcgg ctgctgcagg
     7141 cgctcggttc gccagccgcc atcatcgagg ccaacatgga tcgcttgcgg gcgctgggcc
     7201 tggatggcga gctgctggcc aatctgctgc gcaaccgcga ggccgacccg ccgtggcgga
     7261 cgctggtcga aaacgatctg agctggctcg aacgtgacgc gctgcatctg ttgagctggg
     7321 gcagccctgc ctatccaccg ttattggcac agaccgcgac gccaccgccg ctgctgttcg
     7381 tgcgtggcga tgtcgcgctg ctgcaacgac cgcagttggc ggtggttggc agtcgcagtc
     7441 cgacgccggc cgggatcgag atcgcccaga tgctggcgcg ggcgctggcc gaggcgggct
     7501 tgatcatcac cagcgggttg gcgctgggca tcgatggcgc ggcacaccgc ggcgcgctgt
     7561 cggtgcccga tgggcagacc atcgccgtgg tcggtaccgg gctcgacagt tgttatccgg
     7621 cgcgacatcg gcagctctgc gagcagattg ccgcacaagg ggcggtggtc tgtgaatttc
     7681 cattcggcac gccgccgcgt gccgaccact ttccgcgccg caaccgggtg attgccggcc
     7741 tggcattggg ggtgctggtg gtcgaggccg gactgggcag cggctcactg atcactgcgc
     7801 gccatgcgct ggaggcgggg cgcgagatct tcgctgtgcc cggatccatt ctgaacccgc
     7861 tcagccgtgg tggccatgcg ctgctgcgag atggcgcccg gctggtcgag cgcgccgagg
     7921 atgtgctgga ggagcttggc ctcaccctga gtggccgcgc tgccacggca gccggaccgg
     7981 gcatggctca accacccggg ctgttgaccc cgacggagcg cgagctgttg cgctgcatcg
     8041 actttgagtc gaccagcgtc gacctgttgg tggagcgcag cggactggcg gtcccgcaac
     8101 tgctcggcct gctgtcgcag ctcgaactca agggctggat ccgcgccgga ctgggtggct
     8161 atctgcgcga tccggcgctc ggtgccgacc actgggcgca gttgtgaagg gtggcggtga
     8221 ccggcttgca acctctgcgt ggctcggcta gtttgccggg cccgattcac tgttcaagag
     8281 attccgatgg tcagtcagtg gcgactgctg cgcgccgccc gactgctgcg gcagggtggg
     8341 gtgctggcct atccgaccga agcggtctgg gggctgggct gtgacccggc caaccggcag
     8401 gcggtcgagc ggctgctggc gatgaagcag cgtgccgtct cgcaggggtt gatcctgatt
     8461 gccggcagca tggcgcaact ggaaccgctg ctgcgtgatc tgtcgccttc gcagcgcgac
     8521 gaactggagg ccagttggcc cggtccggtc acctggctga ttcctcacca cgggcgcgtt
     8581 ccgcactgga tcagcggcca tcacgctacc gtggcggtgc ggctcacggc acatccggtg
     8641 gcggcggaac tctgtcggac ctttggtggt atgctggttt cgacttcggc caatccccat
     8701 cgacgaccgc cagcgcgcga tcgtttgcaa ctgttgcgct atttcggcac cgaactcgat
     8761 gagattctgc ctggcgcgct cggcggtgcg gcccggccga gccagattcg cgatctggca
     8821 tcggggcggc ggatacggga ttagctccgt ccatatcatt tcatttgcac gatgatttca
     8881 ggagtcgacg atgagtttcg gcagtgacgg cattgcccgg gtgagtgact atttgcaggg
     8941 tctgcaagat tcgatctgca ccagcttcga acagatcgat ggggtggccc ggttccgcga
     9001 ggagtcgtgg cagcgccccg gcggcggcgg tggtcgcacc cgggtgatcg ccgagggcgg
     9061 gctgttcgag aaaggggggg tgaacttctc ccacgtccat ggcggcaacc tgccgccctc
     9121 cgccacccag caccggccgg agttggccgg acgcagcttt caggcgctgg gcgtctcgct
     9181 ggtgatgcat ccgcgcaacc cctatctgcc gacatcccat gccaatgtcc ggctgtttgt
     9241 cgccgaggcg cccgatgcgc cgccggtgtg gtggttcggt ggcggctatg acctgacccc
     9301 ttactatcct ttcgagagcg actgccgcca ctggcacaac accgccaagg cagcctgtga
     9361 cccctttggc gcgcagcgct atccccgact gaagaagtgg tgtgacgact acttctttct
     9421 gccgcaccga aacgaggcgc gcggtgtggg tgggctcttt ttcgacgatc tgaacgagct
     9481 gggtttcgag cgggagttcg cctttctgcg cgccgtcggc gacagctatc tggccgccta
     9541 tctgccgatt ctggagcggc gccgtctgct cgaatatggc gagcgggagc ggcagttcca
     9601 gttgattcgc cgtggacgct atgccgaatt caatctggtc tgggaccgtg gtacccagtt
     9661 cggcctgcaa tcgggtggac ggaccgaatc gatcctgatg tcgctgccac ccctggtgcg
     9721 ctgggagtat gactaccagc cgccgttcgg cagcgaggag gcgcgtctga ccgaggagtt
     9781 cctgcgtccc cgcgactggg ccacgacctg agaccggagc gcgcatgaaa cagtttgccg
     9841 tcatcggcaa cccggtggcg cacagccggt cgccgcagat ccatgccgcc tttgccgggc
     9901 agttcggcat cgaactcgat taccggcgca ttctgaccga ggcagccgat ttcgccgagc
     9961 gggtgacgac cttctttgca gaaggggggc atggactcaa cgtcaccctg ccgttcaagg
    10021 aggccgcctg tgcgctggcc gatgagctgg gaccgcaggc cgaggctgcg ggtgcggtca
    10081 acacgctgtc actgaccggc gacggacggc tgcgcggtga caacaccgat ggcatcggcc
    10141 tggtgcgcga cctgaccgac aatcttggca tcccactgct gggacagcgg ttgctggtga
    10201 tcggcgccgg tggggcggtg cggggcattc tgccggcact gctggctgcg gcgcccggtg
    10261 aactgctgct ggtcaatcgg acgcggtcgc gggccgatgc gctggtcgag cgtttcgcgg
    10321 cgctgggcag cctgcgcagc ggcgatttcg ccgaactggc aaccgagcag tgtgatctgc
    10381 tgattcaggg cagcagcgcc gccttcagtg gtgaagggct cgcactgcct gccagtctgg
    10441 tggctggcgg cagcgtctac gacctggtct atggcgacaa ggcggcaccc tttctggact
    10501 gggcggcgcg ctgcggtgcc cggcgggtgg ccgatggact cggcatgttg gtcgagcagg
    10561 cggcggagtc cttccgcatc tggcatggcg tgcggccgca gacggcgccg gtgatcgcgc
    10621 aactgcggca gcactgacca ctgatttcca tcgaggtgat tgtcatggac aacccgctgt
    10681 tgcagcggca tgagttccca cccttctcgg ccatcgcacc ggcacagatc gggccggcgt
    10741 tcgagcagat tcttgccgag aaccgtcagg ccatcgcgcg gctggtcagc gcgcccgaca
    10801 gcgacagttg ggccgggttg atcgagccgc tcgatgagct ggccgaccgc atcggccaag
    10861 cgctcgcgcc gatttcgcac ctcaatgcgg tggtgagcga tgaatccctg cgacaggcct
    10921 acaatgcact gctgcccaag ctggccgact acaacgcgga gtatgaccag aatccgctgc
    10981 tgttcgcggc ttaccagcgg ttggcccagt cgccggccca tgccgacctc gatgccacac
    11041 agcagcgcat catcgagcat gccctgcgtg atttccgtct cggtggcatc gatctgccgg
    11101 cagctcagca gcagcgctat cggcagatca gcgcgcgtct ggcggagctc agcagccgct
    11161 tcagcgatca gctgctggat gccaccgacg actggagcct gaccctggac agcgccgagc
    11221 gattgcgtgg cgtgcccgag ctggcgattg cggcagcgcg cgagcgggca cagggcaaag
    11281 gcgaggctgg ttatcggctc acgctggagt ttccggtcta tcaggccgtc atcacccatg
    11341 ccgatgaccg ggatctgcgt cttgagatct accgcgccta ccacacccgt gcttcggagc
    11401 agagtgcgca atcgccgcaa cgcgacaatg gtccactgat cgaggagatt ctggcgctgc
    11461 gcgccgagca ggcgcggatg ctcggcttcg ccagctacgc cgagctctcg ctggccacca
    11521 aaatggccga ttcgccggag cgggtcgagc agttcctgcg cgaactggcg cagcgctcgc
    11581 gcgccaaagc gagcgccgac catgcggagc tggcccgttt tgccgccgcg gacggcatcg
    11641 atcggctcga agcctgggat gtcgcctact acagcgaaaa gctgcgcgag tcggcctttg
    11701 ccatctcgca ggaggccttg cgcccctatt ttccgctgcc gcgggtgatg gcggggcttt
    11761 tcgagctgat cgaccggctg ttcgcggttc gcttcaccga gcagtcgcag ttcgaccgct
    11821 ggcatcccga tgcccgtctg tttgcgctcc acagcgatga tcgactgctg ggccacctct
    11881 atctcgacct ccatgcgcgg ccacgcaagc gcggcggtgc ctggatgggc agcctgcgca
    11941 gccgttttct ccggccgcag ggcgcattgc aaagaccgct ggctgcggtg gtctgcaact
    12001 tcgatccacc ggcgccaggg ctgccggcac tgttgaccca caacgaggtg ctgacgctgt
    12061 tccacgaatt tggccatgcg ctgcatcacc tgatgaccga ggtcgaccat ccggccgcag
    12121 cgggcatcaa tggtgtggag tgggatgccg tcgagctgcc gagccagttg ctggagaact
    12181 ggtgctggca gtccgaggcg ctcaggctga tctctggcca ctaccagacc gaagagcccc
    12241 tgcctgacga attgctggcc cggttggcgg cggcccgcca cttccagagc gggatgcaga
    12301 tggtgcggca gatcgaattc gccctgtttg acctctccat ccacagtgcc gggcacggct
    12361 tgagcgtggc cgaggtgcag gcgcagctcg accagatcag ggctgaggtg gcggtggtga
    12421 acccaccagc gtggaaccgc ttccagaatg gcttcagcca catcttcgcg ggcggctatg
    12481 cggccggtta ctacagctac aaatgggcgg agctgctctc ggccgatgcc ttctcccgct
    12541 tcgagcagca aggattgttc gaccgtgaga gcggccaggc ctttgcgcgc gagatcttgc
    12601 ggcgcggcgg ttcgcggaat gccttggatg gtttcatcgc gtttcgcgga cggccgccac
    12661 agattgaatc gctgctgcga cacagtggta tcggatcgga gagcgaggcc aatggttgat
    12721 cagccgcctg tacgaaagcg tttcatcgcc ggggcgctct gcccgcaatg ccagcagatc
    12781 gacacattgc agatgtggga ggaggcgggt gtgccgcatc gcgcctgcgt tcgctgcggc
    12841 catcagctgg ccatgaccgc cgagtcgctc accccggtct ggcctgcggg tcggcttgag
    12901 cagcgtgcag ccccgcagat cgaggggcag ccgctgattt tctttcccaa accacccgcc
    12961 cggaacgatg actgatattt tgctgaaaat tgatcaagat caaggcattt tgcttcgata
    13021 tcggtagaga atcagccctg ctgagcaagg tcgtcgtctg ttcggcagac aaccataaga
    13081 ataaagcagt ctgtacgcat gtgtcttggc ttgcgtcagg aacaaaggcg caagccggtt
    13141 tctttgtcac atcgggttga gaaaaaacca gcggctgcta tcatggcgcg gcattttgtc
    13201 gcacccggac gtctcgtcga tgcgcccggt gtcgagcaga attaagaaca actcgaacat
    13261 gattcacaca acaacgctaa atggaggcag gacgatgctg ctcggtgcat ggtcgcggct
    13321 caaaactctg atggggctgt tgctcttctc ttcaatcgct cttgccgaag agccgctgtc
    13381 gaagctcaac ctgcgaccag gtgtgaccga agtcagccaa aaggctttcg atctgcacat
    13441 gatcatcctc tggatctgtg tggcgatcgg cgtggtggtc ttctccatca tgttctggtc
    13501 gatcctgatg caccgcaagt ctctgggggt caagccggcc gacttccatg agagcaccaa
    13561 gatcgaactg ttgtggacca tcatcccggt cttcatcctg gtggcgatgg ccattccggc
    13621 gaccaccacc ttggtgtcga tgtatgacac caaggagagc gatctcgaca tcttcatcac
    13681 cggctaccaa tggaagtgga agtacgagta catggggcag gatgtctcct tcttcagtgt
    13741 gctggcgacg ccgcagaatg agattcacaa ccaggaggcc aagggtgagt tctatctgct
    13801 cgaggtcgat cagccgctgg ttgtcccggt tggcaagaag atccgttttc tggtgaccgc
    13861 caaggatgtg attcacgcct ggtgggtgcc ggatcttgca gtcaagaagg atgcggttcc
    13921 ggggttcgtc aacgaggcct ggacgagaat cgacaagccg ggcgtctacc gtggtcagtg
    13981 tgccgagctg tgcggcaagg accatggctt catgccgatc gtggtggtgg ccaagagtca
    14041 agaggattac gacaagtggt tgtccgagcg caaggaggtt gcccgcaagg aggctgagct
    14101 gaagagcaag cagtggacgc tcgccgagtt gacggcgcgt ggagaagagg tatatggcaa
    14161 aaactgtgct gcctgtcatc aggtcaatgg tgcgggtgcc ccgccggcct tcccgccgct
    14221 caagggcagt aagatcgcca cgggggaccg tgccgagcac atcaaaatcg tggtcaacgg
    14281 caagccgggc acctacatgg cagcttatgg gccgcagttg agtgaagttg acctggcggc
    14341 ggtcatcacc tacgagcgca atgcctgggg caacagcacc ggtgaccagg tgaacccgat
    14401 cgacatcgtc aacttcaaga attcgaaata aggagggctt ctgatgggta cgttaaatcc
    14461 cgcagtcgag ggtcatcacg acgatcatca tcatggcccg gccaaggggg ttgctcgctg
    14521 gctcttcgcc accaaccaca aggacatcgg gtcgatgtat ctgtggctca gttttgcaat
    14581 gtttctgctt ggaggtgcct ttgcgctggt gatccgcgcc gagctgttcc agccgggtct
    14641 gcagttggtt cagccggagt tcttcaacca gatgaccacg ctgcatggtc tggtgatggt
    14701 cttcggtgca gtcatgccag ccttcgtcgg cctggccaac tggatggtgc cgctgatgat
    14761 cggcgcgcct gacatggcgc tgccgcgatt gaacaatttt tcattctgga ttctgccgtt
    14821 tgcctttctg acgctggcct cgacgctgtt catggagggc ggggcgccaa atttcggctg
    14881 gaccttctat gcgccgctct ccaccaccta tgcgccgccg agcgtaaccc tgttcatttt
    14941 ctcggtgcac atgatggggg tttcgtcgat tctgggggcg atcaacatca ttgccaccat
    15001 cctcaacatg cgtgcgccgg gcatgaccct gatgaagatg ccgctcttcg tctggacttg
    15061 gctgatcacg gcattcctgc tgattgcggt gatgccggtg ctggcgggtg tggtcaccat
    15121 gatgctgatg gacattcact tcggcaccag cttcttcaat gcagcgggtg gcggtgatcc
    15181 ggtgctgttc cagcatgttt tctggttctt cggtcacccg gaggtctaca tcatgattct
    15241 gccctctttc ggggtggcct cgcagatcat tccagtgttt gcccgcaaga agctgttcgg
    15301 ttatgagtcg atggtctatg ccactgcgtc gattgccttc ctctcgttta tcgtctgggg
    15361 gcatcacatg tttacggtgg gtatgccgtt ggccggcgaa ctttattaca tgtatgccac
    15421 catgctgatc gcggtgccca ctggcgtaaa ggtcttcaac tgggcggcga cgatgttccg
    15481 tggcgccatg accttcgaaa cgccgatgct gtttgcgatc gccttcgtcg tgctcttcac
    15541 catcggtggc ttcacgggcc tgatgatgtc gatcgcgccg gtcgatttcc agtaccatga
    15601 cacctacttc atcgtggccc atttccacta tgtgctggtg ccgggtgcag tcttctcgat
    15661 cttcgctggc gcttacttct ggttgccgaa gtggactgga cacatgtatg acgagcgtct
    15721 tggcaagttg cacttctggc tctcgttctt ctttgtaaac ctgctgttct ttccgatgca
    15781 ctttgtcggg ctcgcaggca tgccgcggcg gatccccgac tatgcgctgc aatttgccga
    15841 tttcaacatg atggcaagtg ttggcgcctt cggtttcggt ttgagccagt tgctgttcct
    15901 gttcatcgtc atcaaatgca tccgcggcgg tgcgcaggca acggccaagg tgtgggaagg
    15961 tgccgatgag gggctcgagt ggacggtgcc ttcgcctgcg cccttccata ccttttcgac
    16021 tccgccggtg gtgaagtagc gattttcctg tggcaggcag aaaccaatgg aaatgaggac
    16081 ccaaaaaaag cagagtcgac tcctgttgac gctgactttg gttgtcgtgg gcatgtttgc
    16141 cttcgggttt ctgctggtgc cgctgtatga cgtgatctgc aggtggaccg gtttgaatgg
    16201 caagactgcg gggcagtacg aactctcatc cgcagaggca gtgcaggtgg atcagagtcg
    16261 gttggtcacg gtacagttcg tggctaccaa caatgaccgc atgtcatggg agtttcgtcc
    16321 gatggtggcg tcgattcagc tccatcctgg tgaagtcaag gaggtgaagt tctacgcgcg
    16381 gaatcccgaa gcgaaggcca tgaccgcgca ggcgatcccg agcgtctcgc cgggcgcgac
    16441 tgcggagtat ctgcacaagg tcgagtgttt ctgctttgcc cagcagcatc tgatggcggg
    16501 gcaggcggtc gagatgccgt tgcgattctt tgtcgatccg aagctgccgg cgcatgtcac
    16561 caagctgacg ctttcgtaca cgctttttga tgtcgataca cttgcgcatg gtgctgaagc
    16621 cagggcgcaa caatcagaat caaccaacta aaacaatact ggccagccag gcaaatgaag
    16681 atggagattt ccatggcaaa gacgacagaa acgcctctgt attatgttcc cgagcagagc
    16741 aagttgccgg tgctgacagc gctggctctt ttttgcatgc tgataggcgg cgggaacttc
    16801 attacggcaa acagccatca gactgattcg actttcgcgt tttggtggat gttgtccggc
    16861 tttgccatga tggcagtgat tttctatgcc tggttcgggc aggtgattcg agagaacgat
    16921 tccggtcttt acagtgatca gctcaaccgt tcctttgtca tgggcatgag ctggttcatc
    16981 ttttctgagg tgatgttctt cgctgccttc ttcggagcgc tcttctatgt gcgcaccttg
    17041 gtgatgccat ggctggcggg tgagggtgac aagggtgtca gccacatgct ctggcctgat
    17101 ttccaggcgg tgtggccgat gatcaacatg ccatcggcaa cagggttctc ggtgccttcg
    17161 caagtggtcg acccttggca cctgccgctg atcaacacca ttctgctggt ctcctccagt
    17221 gtgaccttga cctgggcgca ccatgcgctc aaggccgagc agcgctccaa gctcaatttc
    17281 tggctgttcg tcacggttgc gttgggtgtt tcgtttctcg tgctgcaagc ctacgaatat
    17341 tacgaggcct accatcacta cggcctgacg ctcaattcag gaatctatgg tacgactttc
    17401 ttcatgctga ccggtttcca tggtgcgcac gtcaccatcg gcaccctcat gttgattgtc
    17461 atgcttctgc gagccctgaa agggcacttc aagccaaatg accatttcgg tttcgaagcc
    17521 gccgcatggt actggcattt cgttgatgtg gtctgggtcg gcctctttat ctttgtctat
    17581 ctgtggtagc aaaccaccgg tcaagtacga acgaagagcc gtgttgcgag cctttggttt
    17641 actgccaagg tgctcgcagt gccagttctc cgctccacaa gccatagatg acgcagagca
    17701 gcaacagcac tgccaggctg actctgattg cgagggagtt gagcagtcgg cggctttgag
    17761 tgtctccgcg gtccttgatg aaaaaataga aaccgctgct caagctggcg agaatggcgc
    17821 agaagagcag cataatggtc gttttgagcc acatttcaga acatcctgag ggttggtcgg
    17881 gttcattaaa tcatgaaagc cgtgtcgaga cacgggaagg gccttatgct ggatcaaagg
    17941 gcgaagtgcc tgaatgggca ggttccttga tttcgaaaga aaagcggcag cgttttttca
    18001 ggttcaagcc caatctcaaa ctgacggcgc ttgcactggt catgtttccc ttgctctgcg
    18061 ccttgggttt ttggcagctt gggcggatgg cggaaaagca gaagattctg gaacagctcg
    18121 agctgcagca acggttgtct ccggtcagca tggaagaatt gcccgatcag cttgagcagc
    18181 tgcgtggtcg acgtgtgaaa atcaccggtg agttcgacaa cgacagggtc atcttgctcg
    18241 acaaccggat catggcagga catgccggtt atgaagtgct ggtgttgttc aggactgcat
    18301 cgggggcaag aaatctgtgg ctcaatcgcg gctgggtggc agcggatggc aaccgccaac
    18361 gactgcctga aattgcgcct gtggttgggc aggtaacggt ggttggagag atctatgttc
    18421 cctatggcgc tgcgattttg ctgggtgcca atcaggcaga gggccgggac tggccgaagg
    18481 tggtgcaggc ggccgaactc tccatgctcc gcgtgctttg gggtgttgag ctctatcctc
    18541 accagattcg cctgctgccg gaacagccag gggtgctgca gcggccatgg acaacggtga
    18601 acgtgacgcc agaaaaacat ctgggttatg cggtgcagtg gtttgcgttg gctgcagcac
    18661 tgttgctgct ctatctgtac tcctcgtgtg ttgttgaaga agaggctgcg tgaaatcaag
    18721 tcaaccggca aaaaatgtaa tgaacgctca gtcgcagcct caaaagaggc catgggtgct
    18781 ttatgcaatt gtactgggca ccgcattgcc ggtgcttgca gcctatctgt ttcattatta
    18841 cgcctcggta tggcagccag gctcgcagac ggtcaaccgc ggagttctgc tctccccgcc
    18901 tttgaatatc cagaatttca aactgctgga tcgggcgggt caaccgttct tcaaagagga
    18961 gtttccaggg aagtggctca tcctgcaaat cgccagtgac gagtgtggtg agccttgtca
    19021 ggtacgtcga catgagttgc gccaggtaca tgtggcactt ggcaaagaga tgaccagagt
    19081 gaatcgtctg ctggtccagg gccgagagga tcggaatttc gttgacgatc agcagacaaa
    19141 agaaaatgag ccgccgttga gggtcgcgta cggtgatctg ctggagatca ggcatcatct
    19201 ggcacagatc gacgccttgc gcagcgttga ggtgagtgaa ctggtgtttc tggtcgatcc
    19261 gctgggcaac atcatgatgt actacacccc cactcacagt ggcaaggatc tgctcaagga
    19321 tctgcagaag ttgctcaagg cttcacaaat tggctgatgg cgcgatggtt actgggttca
    19381 agcgcaaacc gggtttcatg ctggcgttgt gcagcacgtt ttttactctg atcgtggtgc
    19441 tgctgggcgc ctatacacga ctttccgacg ccggattggg ctgccctgat tggcctggtt
    19501 gttatggctt tgtgagcgtt cctcgcagtg aggcccagat cgatctggcg gaaagtcgct
    19561 atcctcatgc gccggtcgag gcccacaagg cctggccgga gatggtgcac cgttactttg
    19621 ccggtacctt gggcttgctg gtattggcca atctgatcgt ggcgtggcgc caccgccatc
    19681 tgccgcagca gccactgggg ttgccgctgg cgctggccgt gctggtggtc gtgcaggcgc
    19741 tgttcggcat gtggacggtg accctgaagc tctggccgca ggtggtgacc gcgcatctgc
    19801 tgggtggctt tgccaccttg acgctgctgt ggctgttgac gctgcggctg tcgaactggg
    19861 gagactcagc ggcggccatc tcaactcgat cggccggtat gcgccggttg actcgtttgg
    19921 gtctgttgct gctgctgttg cagatcgcat tgggtggctg gctgagctcc aactatgcgg
    19981 cacttgcctg tcctgatctg ccgacttgtc aggcgcagtg gtggcccgag atggattttc
    20041 gtgcaggatt caacctgttg caggatgtgg gccccagcta tctgggcggg ctgctgcaca
    20101 acagtgcgcg catggctgtt catgtgaccc atcgactggg cgcattggtc gtgacggcca
    20161 tattgctgct gcttgccttc aacctgtttc gccactccgg agtggcgcga aagctggcgg
    20221 gtttgttgct cctggtcttg acactgcaac tgtcgctggg ggttgggaac atccttctgc
    20281 aattgccact gccgatcgcg gttgcgcaca atggcgttgc cgcgctgctg ctgctgacgc
    20341 tgacggcgat cgaccacttt ttgagcaggc gcgccgccgc cgggagatga gtgtcatgag
    20401 ttcgacctcc atggagcaag ctggaaagcc gatcggcctg ctcgatcagt tcaatgacta
    20461 tctggagctg tgcaagccca aggtggtggc cttgatgatc ctgacctcga tcgtcgggat
    20521 gtttctggcc gtgccgggga tggtgccatg ggatgtactg ctgctcggca acctgggcat
    20581 tgcgctggtg tcggcctcgg gggcagtgat caaccatgtg atcgaccggc gcatcgacac
    20641 cctgatggcc agaacccaca agcggccggt ggccgtaggc cgtgtctctc cagcacgtgc
    20701 catgctgttc gcgttgttga tcggcgtggt cggaatggcg atcctgctga tcttcgtcaa
    20761 tccgctctgt gcctggctga cgctggcggc cttcgtcggt tacgccttca tctacaccgg
    20821 ctacctgaag cgggcgacac cgcagaacat cgtcatcggt ggcattgccg gtgccgcgcc
    20881 gccactgctg ggctggagcg cggtgaccgg gacggtcgat ccacatgccc tgctgctggt
    20941 gctgatcatc tttgcctgga cgcccccgca cttctgggcg ctggcgatcc accgcaagga
    21001 ggattatgcc aaggccaaca tcccgatgtt gccggtgacc catggggacc gttacaccaa
    21061 gctgcacatt ttgctctaca cggccgtgct gttcgcggcg gcgctgctgc cggccgcgac
    21121 cgggatgagt ggctggatct acctggctgg cgccagcctg ctgggagcgg gcttcatcta
    21181 ccgtgccgtg cagctctgtc tggatgaaaa gtcgggcgcg ccgatggcga cctttcgcta
    21241 ctccattctc tatctgatgg cgctctttgt cgtactgttg gtcgaccact actttctgat
    21301 tcggctatga gcggtggggc gactcgcggg aagtggatca ccgtagcgat tctcaccgtc
    21361 gtcgtgaccg cgatcgggct ggttttttcc agcaatgtca ccaagccccg agcgctgtcg
    21421 gtcgagcaac tgcgcaccaa tggcctctat ctgctggagc agcccaagtc actgggtgaa
    21481 tttgcgctga ccacggcaac cggaactgct tttaccgctg ccgaactgcg cggtcactgg
    21541 acgctgctgt tcatggggtt cacccattgc ccggtctttt gccccaccac gctggccacg
    21601 ttgaacgagc tggtcactgg caaactgggt gagacgccct atgcggccga cacccgcgtg
    21661 gtgatggtgt cgctggaccc gcagcgggac agtgcagaga aattggccgg ctacctgaat
    21721 ggcttcaacc ccggtttcac cggagtgacc ggcagtttcg agcagatatt ccagttcagc
    21781 cagagcctgg gcttctcctt cgccaagttg ccgttggcca atggtgatta caccatcgac
    21841 catgccggca tgattgccct gatcgatgcc gacggccatt accgaggtta cttcaagccg
    21901 ccgcatgatg cggcgcagat gctggtcacc taccgttctg cccgtgccgg gctgctgcgc
    21961 tgatctgcgg ttcacacgag caacttgacg ccgatcaata ccaacatcga ggcgagcact
    22021 ggccgcagca gccgctccgg catccgcgcg gccaggtggc tgccgaggta gatgcccggc
    22081 agcgagccga tcagcagact gccgagcagg ctccagtcga cgctgcccat catccagtgg
    22141 ccgatgccgg ccaccagcgt cagaggcaca gcgtgggcca gatcggaacc gacgatgcga
    22201 atcgccgaaa gttgcggata gagaaagaac agcaccgcga caccaatcgc gccggcgccg
    22261 accgacgaca gtgacaccag cacgcccagc agtgcaccgg ttgcgacagt gaccgggccg
    22321 agccagcgcg tgcgccagtg gtcactgtgg tgactgagtg cgaacgcctg aattctctgc
    22381 ttgaaaatca gggccacggc ggtcagcagc agggcgacac ccagcaccga ggagatgacc
    22441 accgacaggt gcttctccgg caatccaaaa taactgagcg ccgagagcgt gaggatggtg
    22501 gccggaatgc tgccagccgc cagacggccg gtgatcttcc agtccacatg ccccttgcgg
    22561 gcatgcaccg tggtgccgcc catcttggtg atggccgcat agagcaggtc ggtcccgacg
    22621 gcggtggcag ggtggacgcc gaacagcagc atcagcagcg gtgtcatcag cgagccgccg
    22681 ccgacgccag tcacgccaac gatggcgccg acgacaaaac cagagaagat gtaggcagat
    22741 gtcatgagct ggggcacggt tccatcagaa gatggcgtga tgctagcagc cccttataat
    22801 tctaaaaaga ataaaagatt atagaggata taaaaaaaga ataagagggg ctgtcaatct
    22861 gcgcagcggc ggcatggttg ccgattcttg atgaacagga ttgctgactg gatggtgcgc
    22921 agggtgtggc aaatggccca agggacaaga gtgccgatgg gcgatcactt ccaggtgaaa
    22981 atctcccagc tcggaatcag catgttgggc tgatcgatgt tctggaaatc cttgccgttg
    23041 gtggtgacca gaaaataggg cttgccgttc ttgggaatca ccttgacggc gtaggtgaag
    23101 ccgttgatcc ggtattcatg gatggtttta tccttgcctt cgtggatgat cacctcggcc
    23161 tctcctggcg cggcctcctc ggtgaaggca ggcagggcaa ggcagagcgc aaacagaatc
    23221 gacagcaggc gcaaagatct catgatgggg tccagcagtg ggtgcacggc gaatcttacc
    23281 actgtagtct tggcgttatc ttccgatctg aaggattcgt tgcagaaaaa ccatgagtga
    23341 aacccagaac aggccgcggc agctggtgct ggtcgatggc tcttcctacc tctatcgcgc
    23401 ctattacgcc ctgccgccgc tctccacccg tgacggagcg ccgaccggtg cggtcaaggg
    23461 ggtgatctcg atggtgcgca agctgctgaa ggagtatccg gacgccacgg tggtggtggt
    23521 cttcgatgcc aagggcaaga ccttccgcga cgagctgttt gcccagtaca aatcgcatcg
    23581 gccaccgatg ccgaccgatc ttgcccagca gatcgagcca attcagcaga tcatccgggc
    23641 gctggggctg ccactgctgg tggtggaggg ggtcgaggcc gatgatgtga tcggcaccct
    23701 ggcgcggcaa gcgacagaga tgcagattcc gacagtgatc tcgaccggcg acaaggacat
    23761 ggcgcagctg gtcaacggcc atgtcacgct gatcaacacc atgaccgaca cccggctcga
    23821 ccgggccggg gtggagcaga agtttggcct gccgccggag ctgatccgcg actatctggc
    23881 gctggtcggt gacagcagcg acaacatccc cggggtgccg caggtgggtc ccaagaccgc
    23941 ggtgaagtgg ctgagcagcc atggcgatct cgatggcctg ctggcacagg ctgcggagat
    24001 tcccggcaag gtcggggaga gtctgcgtct gcatctcgat caactggcac tgtcacggca
    24061 gttggccacc attcgctgcg atctggaatt accgatcgat ctggccacgc ttcagtacgg
    24121 tgaagtcgat cgagcgcggc tggtcgagct gttcgcgcaa ctggagttca acagctggct
    24181 cgatgagtac cgcgacaagc agcctgcgtc agcgagccat ctcccgctgc ggcacgagac
    24241 cctgctgaca gcggagcagt tcgagcgctg gctgcagaag ttgacggcgg ctgcgaattt
    24301 cgtgatcgac accgaaacca gcagcctcga ctgcatcagc gcgcaactgg tcgggctcgc
    24361 ctttgccgtc gagccgggtg aggcggccta tctgccactg cggcatgact atcccgacgt
    24421 gccgccccag ctcgactgcg attgggtgtt gtcacggttg cggccactgc tcgaagatcc
    24481 gggccatggc aagatcggcc agaacctgaa gttcgacctc ggcatcctgg cccgccatgg
    24541 catcgcgctg cgcggcatcg ccgaagacac catgctggaa tcctatgtgc tcaacagcac
    24601 cgccagccgc cacgacatgg acagcctggc cctgcggtat ctgggtcggc agaccatttc
    24661 cttcgagcag atcgccggca agggcagcaa gcagctcagc ttcaaccaga tcgcgctgga
    24721 gcaggcggcc ccctatgccg ccgaggatgc cgaggtgccc cttcggctgc accaggttct
    24781 gggtccgcaa ctcgatgccg aaccccggtt gcggcaggtc tatcgcgagc tggagctgcc
    24841 gctggtgccg atcctgtcgc gcatcgagcg tgccggtgtg ctgatcgatg ccgagcggct
    24901 cacccgccac agcggcgagc tggccgtgcg tatcgccgaa ctggagcagc aggcctggca
    24961 actcgccggg gccaccttca atctcggttc gcccaagcag ctgcaggaga tcttctacga
    25021 gcggctccag ttgccagtgc tgcaacgcac gcccaccggg cagccctcga ccgccgaagc
    25081 cgtattgcag gagctggcgc tcgactatcc gctgcccaag gtgctgctgg agcaccgcag
    25141 cctcagcaag ctcaagtcga cctacaccga tcgcctgccg gagcagatcc atccgctcac
    25201 cggtcgggtg cacacctcct accatcaggc tgttaccgcc accggccggc tcagcagctc
    25261 cgaccctaac ctgcagaaca tcccgatccg cacgtcggag gggcgacgca tccgccaggc
    25321 gatcatcgct ccagcgggtc atcagctgct ggccgccgac tattcgcaga tcgaactgcg
    25381 catcatggcc cacctctccg gcgatgccgg tctgttgaat gcctttggcc gtgggctcga
    25441 tgtccaccgc gccaccgctg ccgaggtgca gggcatcccc tacgagcagg tgaccagcga
    25501 agagcggcgc cgtgccaagg ccgtcaactt cggcctgatc tacggcatgt ccgcctttgg
    25561 gctggcgcgg cagttgggca tttcgcgcaa cgaggcgcag ggctacatcg atggctactt
    25621 cagccgctat cccggtgtgc tcgactacat ggagcggatt cggcagacgg cccgtgagca
    25681 gggctatgtc gagaccctcc atgggcgccg cctgcatgtg ccagaaatcc acgcgcgcga
    25741 ccaggcgcgc cgtcgcgccg ccgagcgcac cgcgatcaac gcgccgatgc agggcagcgc
    25801 cgccgacatc atcaagctgg cgatgatcca gctcgatgaa tggcttgaac gcacgcgggc
    25861 accggcacga atgatcatgc aggtgcacga tgagctggtg ctggaggtgg ccaacgaagc
    25921 gctggagcag gtcagacccg aagtgaagcg gatcatggag caggtgacga cgctggctgt
    25981 accgctgctg gtcgagatcg gtaccgggcc cgattgggat gtggcggccc agcgctgaga
    26041 ggcgcaccat tgggaatcga accctcaggt cccaaaaacc gactacgctt taaagccatg
    26101 gagtcggttt tggcaaagcc gttcagtatc gatcgatcag ctctggatgc gtgatgtcgt
    26161 ataggccgcg tgcgtttcaa tcttttccat caactccctg tcgaccaatg aggccgcgcc
    26221 tcgggtgtgg catcgagtga aaatggtcct gcaggacacg accttgccca gtggcggggt
    26281 tgattccacg gcactcactg caaaggtgca tggcgagcag gttcgtctgc tctatgccag
    26341 cctgccgctg tcattgctga tcaacgtcgt caatgcctcg ctgctggcat tcatcctgtg
    26401 gggagccatc gacacgaccc gtctgctgaa ttggctggtg tcgattcagt tggtgacgct
    26461 ggggcgtggc ctgctgctgc tcggctatcg acgcatggca tccgatgtga ctcggcccaa
    26521 ggtctggatt cgagggtttc gcatcggcgt ggtggcggtg ggtctctgtt ggggggtggc
    26581 cggctggtgg ctgttcatcc ccgatgacgt ggcccaccag gcatttctgg ccctggtgct
    26641 ggccggcatg tcggctggtg cgctgagtgc gatgtcggct gaccgaccct cggtgctggc
    26701 gttcatgctg ctgtcgctgc tgccgctgct ggttcagctt atggtcgcgc aagcgtcgat
    26761 ccagcttgtc atgagcgtca tggtgttgct gtttctggcc agcctcatca gcaacgcact
    26821 gcgcattcat cacaccattg tcgagaacct ctgtctgcgt atggaggccg aaccgcgtga
    26881 gcggatgttg cggcagcaag aagagcgtta ccgtgccctg atcgacaccc tgcaggaggg
    26941 ttttcagatt ctcagtcacg actggcgtcg tctctacatc aacgacgctg ccgcgcggca
    27001 gttgcgccgg agccggcagg tgctgttggg tgagttgccg gaacagtgga agaaaagtgc
    27061 gttcttcgcg ttgctcgaac agtgcatgga gcaacgtcag ccgctgcatg tggagcagaa
    27121 gattaccctg catgatggtt cgcagggctg gttcgagctg agcatccagc cggtggagga
    27181 gggcatctcc gtactgtcga tcgacatcac tgctcgcaag gaggccgaag aggagatcaa
    27241 tcgcctggcg ttccacgact cgctgacccg gctgcccaac cgccgtctgc tgttcgaccg
    27301 cctgcagcag gcgttgcggt ccagcgtgca cggccagcga cccggggcac tgctgtttat
    27361 cgatctggac gatttcaaga ccatcaatga caccctgggg catggtgtgg gcgacctgtt
    27421 gctgcgcgag gtggccaacc ggcttgcaca ctgcgtgcgt caggccgaca ccgtggcccg
    27481 tcagggtggt gatgagttcg tggtgctgct cgaggcattg ggacccacgg acgaggaggc
    27541 cattgcgcag gcggagcgcg tggccgaaaa aatccgggaa agcctccttc agccttacca
    27601 gctcgccggt caccatggcc gccactgtac cgccagcatc ggcatcgcac tgttcatgga
    27661 tgctcaagca agtgcggatc aactgttcaa gcaggccgac atcgccatgt atcaggccaa
    27721 gcaggcgggt cgcaatgcca tccgcttctt tgatccgcag atgcaggcga gcatcgagca
    27781 gcgatcgaga ctcgaaaccg aattgcgcca agccgtgcag cagcggcagt tcgttctcca
    27841 ttatcaggtt caggtcgacc acgatggcca gccgcttggt gccgagatcc tgctgcgctg
    27901 gcaccatcct gaacggggcc tcatcgcgcc ggccgagttc attccgctgg ccgaggagac
    27961 cgggctgatc attccgattg gggagtggct gctggaggag gcctgctggc agcttaaaaa
    28021 atgggagcgc gaaccggaca gggcgcagct gcatctggcc atcaatgtca gtgcccgcca
    28081 gttccggcag cccgatttcg tcgagcgggt ggtcggcatg ctgcaacaga ccggcatcga
    28141 tccgcgcaaa ttgaagctcg aactcaccga aagcctggtg cttgacaatg tcgccgaggc
    28201 catcgcgacc atgcagagat tgaaggagag cggcgtgcag ttctcgatgg acgattttgg
    28261 taccggctat tcgtcgctcg cttacctgaa gaagctgccg ctcgatcagt tgaagatcga
    28321 tcagagcttt gtcagcgaga tcgacaccga tccggatgac gccaccatcg tgcagaccat
    28381 cgtcgccatg gcgaaaaatc tcgacatgga ggtgattgcc gaaggggtgg agagtgagtt
    28441 gcagcgcaac tttctgatcg aatgtggctg tctgaacttt cagggctatc tgttcggcaa
    28501 accgctgccg ctggcggagt ttgaattgtt gctgcaggag cttgccggca gaagaaagca
    28561 ggtgacaccg atcaggattg gcgctggcgc tcagtgagca gtccattggc tggcgaggcg
    28621 tcgagctggc cctgcaggag atttgctgca ttgaccgaat gggccccgtt catgggattt
    28681 tgatttcgtg gctcggcttc ttcagtgtaa aatggccgaa agatcgccct ccacagccgc
    28741 tctgcatgcc agaatccgtc agaaaaccga tcgacactgc gcttcagcac gatctgcatg
    28801 ctctggcacg cgatgagttc gccctcgtca atcagaccat cctgcggcag ctccactccg
    28861 acgtcgagct ggtgcgcgag attgggcact acctgatcga aagtggcggc aagcggctgc
    28921 gcccgctggt ggtattgctt gctgccgggg cctgccaata ccggggcagt gcccacatcg
    28981 acctggcagt ggtggtggag tttctgcata ccgccacctt gctgcatgac gacgtggtcg
    29041 acatgtcgca gttgcggcgc ggtcgcacca cggtcaatgc gcgctggggc aatgcaccga
    29101 gcgtcctggt gggtgatttc atctattcac gtgccttcga gctgatggtg aagatcggca
    29161 gcctgcagat catgcaggtg ctggccgaga ccaccaacaa actcgccgag ggcgaggtga
    29221 tgcaactgct gaacgcgggc aaccccgatg tggatgaaac ccgctatcgt gaggtgattc
    29281 tgcgcaagac cgccaagctg ttcgaggcct ccgcgcagag tgcggcgatt ctggccgggc
    29341 gtcccgcgac agaggtcgag gcgctggcca gttatggtcg ccatctgggt ctggccttcc
    29401 agttgatcga cgacgtgctc gatttcgatg gcaacagcga cctgctgggc aagaatctgg
    29461 gtgatgatct ggccgaaggc aaaccgacgc tgccactcat catcacgctc gaacgcggcg
    29521 cgcccgccga tgccgcgctg gtgcgcgaca ccattttgaa tggctccagc aacgcgctga
    29581 atgcggtcat cgacgcggtc aagcgctccg gtgggttgga atatacccgt gaattggccc
    29641 gccatgaggc cgaaagtgcg atagaatcac tgcacgttct cgcgccgtcc cgccatcggg
    29701 atgggctgat cagactggcc cgctttgcgg tcgaacgtca gtactgatcg atctgtttca
    29761 gaccattcgg ggtgtagctc agcttggtag agcactgcct tcgggaggca ggggccggag
    29821 gttcaaatcc tctcaccccg accaatggtc tgactgcgcg aacagttgat ggaatggaat
    29881 ctgctcgata ttcgtagtaa aaagagccca ttccccagta gccggactca tgcccacagg
    29941 ccagctctac atcatctccg cgccttccgg tgctggcaag accagcctgg tcaaggcgct
    30001 gttggagcag gactctgatc tggcggtcag cgtctcgcac accacccggc cgcgtcgggt
    30061 tggcgaggtc gatggtgtca actaccactt catcaccacc cagcagtttc tcgatctgct
    30121 ggcgcaggat gcctttctcg aacatgccga ggtgtttggc cactactatg gcacgtcgca
    30181 ccttgcggtt gcacggcagc tcgaacgtgg tcaggatgtg attctggaga tcgactggca
    30241 gggtgcgcgg caggtgagac gtctgatcga cgatgcggtg tcgatcttca ttctgccccc
    30301 ttcgcgggct gaactggaac ggcgtttgac cgcgcgtggg caggatgggg tcgaggtgat
    30361 cagtgcgcgc atggccaagg ccaccgccga gatgtcgcac tatccggagt tcgattacct
    30421 gctgatcaac gactctttcg agcgggccct gaccgagttg caggcaatcg tgctggccaa
    30481 gcggctggag ttgccgcgac agagtgacaa ggcgcgcaac ctgatcgccg aactgttgtc
    30541 atgacagaga gccacatggc attgaatctg gctcattcga gccgtaacga tgtgacagaa
    30601 agaggtttat atggcaagaa ttaccgttga ggattgtctg ccccatgtcg ataaccgttt
    30661 cgatctggtg atgctggcct ccaagcgcgc ccggcagttg gcagtccagg gtcgtgagcc
    30721 cttggtcccg tgggagaacg acaagcccac ggtggtggct ctgcgcgaaa tcgcggccgg
    30781 actgatcgac cgtgactatg tgaatcggct cgaacgtgaa gacgagcagc acagtgtgct
    30841 gttcgacgcg atgccctagg atcctgcgtc gaagagcgga gcaaaggtga tgaacggcgc
    30901 accgaccatc accgcgacag cagtcgacag cccgaccgac ggacgcgagg ccgggccggt
    30961 gtcccctggt cttgcgcgtc tcattgccac cttggctgat tatctcgacg ccgcaaggat
    31021 cgacgagata atccgcgcct accactatgc cgccgcggcg catggctgcc agacccgccg
    31081 cagcggcgag ccctacatta cccatccggt cgcggtgggg cagatactgg ccgagatgca
    31141 catggaccac catggcatca tggcggccct gctgcatgat gtgctcgaag acaccagtgc
    31201 cgatgagcag gagctgcgga ccctgttcgg tgacaccgtg gccgagctgg tcgatggtgt
    31261 cagcaagctg cagcggatga atttcgccag ccaggccgag gcacaggccg agaactttct
    31321 gaaaatgacc atggcgatgg ccaaggacat ccgggtcatt ctggtcaagc tggccgatcg
    31381 actgcacaac atgcgtacgc tgggcgtgct ggcgtcggcc aagcggcgac ggattgcccg
    31441 ggagacgctg gagatctacg ctccgatcgc ccatcggctg ggcatgtatg agattcgcgc
    31501 cgaactggag gatctgggct ttcaggcgct ctacccgatg cgggcacgtc gcatcgaggc
    31561 ggcggtgcgc aaagcgcgtg gcaaccgcga cgagatgctg gccaccctgc ggcaggcgat
    31621 ccaggcgcgg ctggagcaga ccgggctgcc gtgccgggtc atgggtcgtg agcgtcacct
    31681 ttacagcatt taccgcaaga tgcgtgaaga gcgcaaatcc ttcaacaaga tcatggacgt
    31741 ctatgccttt cgggtcatcg tcgaccaagt ggacagctgc taccgtgcgc tcggtgtgtt
    31801 gcatggactc tacaaaccgg tgcccgggcg tttcagagac tacatcgcca tccccaaggg
    31861 caacggctac cagtcgctgc acaccacact gatcggcctg cgtggcgtgc cgatcgacat
    31921 ccagatctgc accgaacaga tggaggccat ggcgcaccat ggtgtggccg cacgacgttt
    31981 tctggatgga tgcagcgaga gcccgtccag tggtgaggac cgcaccaacc agtggctgca
    32041 ggggctgctg gagatgcaga aggttgcggg agattcgatc gagttcatcg aaaatgtgaa
    32101 gatcgatctg ttccccgacg aggtctatgt ctttaccccg aaaggaaaga ttctcgaact
    32161 gccggtgggt gcaacggcca tcgactttgc ctacgccatc cacaccgaca tcggccacag
    32221 ctgtgtgggc tgccagatcg accagcgtct ggctcccctc agcgagcagc tcagaagtgg
    32281 ccagacagtg aagatcatca cctcgccggg cgccaggccc aatgtcagtt ggctcaactt
    32341 cgccgtcacc gccaaggcgc gcagcagtgt tcgccactac ctgaagaacc agcaatatac
    32401 cgaggccgtc gacctcggcc gcagactgct gcaaaagagc ctgtcggtct gccagaagag
    32461 tctggacgaa ctgccacagg cgcagatcga tcagctgttg caggagtatc gcctgaacgg
    32521 gctggatgcg ctgctgagtg aaatcggcct gggcaaccgt ccggtgcagg agattgtgcg
    32581 aaaactgctg cccggagtca gcggcacgct tcaactcggc atggtcgaca gcggcaccaa
    32641 tgccctgatc atcggcgaaa agggcgggat gctgctcaac tatgcgcgct gttgctatcc
    32701 gattcccggc gatgcggtgg tgggccagct cagcagcggg cgtggcatca ccattcacca
    32761 ggagacctgc cggcatgtcg aacccattct gggcgacagc ctgcattgcc tggcggtgca
    32821 gtggcagagc gggatcgagg gtgagttcgt cgccggactc agagtcgaga tgaaaaacaa
    32881 gcgtggcatg ttggccagcg tggccagtgc catcgccgac agcgacagca acatcgagcg
    32941 ggtgaccacc gccgatcggc atggcgacat ctcgatgctg gtcatcgagg tgagcgtgcg
    33001 tgatcgggtc cacctggcgc gggtcatccg caagatcaag gcgatcgacg cggtcagccg
    33061 cgtctttcgc agcagaagtt gagcaacctc aggaagcacg atgaatcgac gcatcatccg
    33121 gaccgatcag gccccgcagg ccatcggtac ctactcccag gccgttgaag cccaaggcac
    33181 cctctatgtt tcgggtcaga tcgcgctcga tccggtcacc atggtgctgg tcaacgactc
    33241 gatcgagaac gagatcaccc aggttttcaa caacctgcag gcggtggtcg cggccgctgg
    33301 catgaccctg gatgaggcgg tcaagctcaa catctacctc accgatctga ttcattttgc
    33361 caccctcaac cagatcatgg cccgcttctt caatgagccc tatccggctc gcgccgcagt
    33421 gggcgtggcc cagttgccac gcggtgcgcg ggtcgaggtc gatgcagtgg tggtcaggcg
    33481 gtaagcctgc tgcggccgac gcctgccaag tgcatgtcga caggacagtt tcagaacgga
    33541 atgtcgtcgt caaagtcatc gaagcggccg cccatgtcgg catcggcgtt ggcgcggctg
    33601 ccgccgccac tggattgacg tggcgacatg ggcgggcctt cgccgtagtc accaccatag
    33661 ctctcctgtc caccgccggc gcggccgtcg agcatctgca tctcgttggc gtgaatctcg
    33721 gtgctgtagc gtttttcgcc gtttttctcc cactgctggg tgcgcagcga gccttcgatg
    33781 tagaccttgg agcccttgcg caggtattgg ctggcgatct cggccaggcg gttgaagaag
    33841 accacccggt gccactcggt gcgctcctgg cgctgcccct gtttgtcggt ccagctgtcg
    33901 ctggtggcca gacggatgct ggtgatctgg gtgccgctgg cggtcgagcg cacctccggg
    33961 tccgcgccca gattgccgat cagaatgacc ttgttgatgc ctcgggccat gggggttctc
    34021 ctgtggtcac gcgcgcgatc ggatgtcacg catcgagcaa tgataaccgg tggcgggcat
    34081 ggtcatccaa ccgctgccgg tcgagtttga ggtaaatctc gccgctgtcg ggaaagagtg
    34141 ccacttccgc aacgccggca agcgcacgca gttcatgcag cagcagttcg gcttggtagg
    34201 gatcctcggg agcgggttca tcaagccgca gatcgagcag cgtgcgcggt ctcttcatgc
    34261 cgcaggcgag caggaaccag agcaggccga tgccggccag cgccaggaac agtggctggt
    34321 agccgcggct tgctgagcac cagtgcatca gggccccgcc actggccgcg ccgaaaaagg
    34381 cgccgagaaa ctggctgctg gaggacatcc ccatcgccgt ccccttgctg ccggccggcg
    34441 ccatcttgct caccagcgag ggcagggtcg actccagcag gttgaagccc atgaagaaga
    34501 caaacagtgc cgcgaccaca gaccagaggt cgctctgatc gaacgacagc agcagttggc
    34561 tgatgatcac cagcgccagc gcaccgagaa agaccggctt catctgctgc cgggtttcgg
    34621 caatgatcag gaacggcaac atggcgaaga aggacacgcc gatgatcggc aggtagtagt
    34681 gccaatgctg accgactgga atcgacagtt gttgggtcag aatcatcggc agcacgacga
    34741 agctggccat catcatgaag tggagcgcaa agacaccgaa gttgagccgc aacagctcgc
    34801 ggtcagccag cacctgtccc agctgccccg gcacggcctc gacgttgcgg tggatgatct
    34861 gctggcgcgc cttgggcacc agcagcagca ggatcagttc gccagcgatg gccagcagcg
    34921 cgctgatcca gaaaatgccg ctcaggccgc tgacgtgggt caccatcggg cccaggatca
    34981 aggcggccga aaaggagagg ccgatgctga cccccatgat ggccatcgat tgggtgcgca
    35041 cctggtcgcg ggtcagatcg gccagcaggg ccatcagcgg gctggcaatg gcgccgctgc
    35101 cctgcagcag gcggccggcg atgatgccgt agacgctgga cgacagcgct gccaccacgc
    35161 tgccggcgat gaacagcagc aggcccagcg agatcaccgg ccggcgaccg atgcggtccg
    35221 acaacaatcc gaagggaatc tgcagcagtg cctgggtcag cccatgcgca ccgagggcca
    35281 atcccatcag cagcggtgtg gcgtcgcgca gttcggactg atgcagggtc agcaccggca
    35341 gcaccatgaa cagcccgacc atgcgcagcg caaagagcag cgagagcgcg agggtcgcgc
    35401 gaagctcggc ggagttcatc gggcagtgat ccggacagcg ggcagcgccg gcatctgcat
    35461 cgacaggtga cgctggcaag gagaccgatg gtgggaacag gcgtgtggct tcatggtacg
    35521 gcagtggcca gcgaagaccg atggcgaatg gatcgtgtgg tcaatcaatc agtcatccgc
    35581 gcgccggcct gtcgcaacgc gatcagatat tcctctccct tgggatggcc ggcaatgatc
    35641 cgggccacgc tttggccacg tggatcggtg gcatcgaggt catggcccgc cgcgatgaag
    35701 tggccgatga agcggctgaa atcctcggcg atcatgctcc ggtaggcctg cagcagccga
    35761 tggtgatcgg ctgacaggcc ttcagggggt tgcagatcga gcaggctgcg cagatgttca
    35821 tcgctccagc tctggccgat gacggccttt ttgtccttgc gtttgggggg tgtggtggtc
    35881 atgtttcaaa accagaaagc agatggcagg aaaaagcgga tgaaaaaccg ttgaatcaat
    35941 cgcccggccg cggcagcagc ttgctgcgca gccagccaag caggctgttg ttcttcagca
    36001 gattgcggtc gatgtgctcc agttcgcgct ggagctgctc aggattacgg aacagctcgg
    36061 cacgggtggt gatcggaatc tgcggatcaa gctccttgac cacccgcgcc ggattgccga
    36121 cggcgatcga gttgggtgga atgtccttgg ccaccaccgc accggcaccg atgatgctgt
    36181 tgtcgccgat ggtcaccccc ttgcagatga tggcgctgtc accgacccag acattgttgc
    36241 ccagcacgat cggtgcagtg gcgccaatgg ccttctcgcg gtggtagatg ccgtgccagt
    36301 cggcgtcggt cagataggcg ttgtaggcga acatgcagtc gtcgccgatc tggatcatcg
    36361 aagcggacga catgcgcacg ccgggggtga tcagcacatg cttgccgatc tcgatgcggc
    36421 ccttgcccag caccgtcaac tgcacctggg cattgggggt gcagatgatg tgggcgaagt
    36481 caccgatcga gatcggttgg ccatagatca gcagatgcca cggcttcagg atcgcatgac
    36541 cgtggccgag atgggtcaat tgcggcgcga cgaaatggcg cgtatagaag ctctgcaccg
    36601 ccatgtgcag tcgcttgatg tagtaggggc ggtgttcgtt gatcaagatc cgttgccgtc
    36661 aactgaattg aaggcggcat gatacggaag ttgccgcggt gacggtagcg ctcgaggcgc
    36721 ggatcgggcg tcgaggggcg ttctgctctg gcgcggcggg ccgatgagca aaaagatgtg
    36781 cgaccacccc gatccggctg aagcggggtg gtcgccgaca actgtggtgg gctattcgac
    36841 cttgacgtcg attgccttgg gcgtgctctt ctggctcttt tcgagatgca ggttcagcat
    36901 cccatccttg aaggttgcct tgaccttgtc tgggtcgaca ttctccggca gggagaagct
    36961 gcgaacgaag ctgccgtagt ggcgctcgat gcggtggaac ttcttgcctt tttcctcttt
    37021 ttcctgcttt cgctcgccgc ggatggagag cacgccgttc tccacgccga tcttgatgtc
    37081 ctccctgttg acctcgggca gctcgacctt gatctcgaag gcctggtcag tctcggcgat
    37141 gtcgaccttc ggcagccagt cgccggagac cagctcctgg ccgatgttgg ctcttggcca
    37201 gcccatcgcc ttgctgtagc ggtcgaacat ctcttcgatt tcgcgcagtg gctcccattt
    37261 taccaatgcc atgatgatgc tcctgtgctg aggcgggact ccctttgcgc gcagtggccg
    37321 gtcccgttgc agccgctgcg gcgccgtgct tatcacacac tttgcatatg gcatctctcc
    37381 ggtcgatttc aagccgcagc tcagggattg cggttgaact ggattggctg gcgcagctgt
    37441 ggatggccat cgcggtcgcg aatctgcacc tccacccggg gctgattgtc ttgccagtcg
    37501 atcagcagca caccatggtt gaggccggtg taaggttcgc cgatgcggta acggttgggt
    37561 tcgccgggaa aattctccca actgtgggtc aacccgctcg aagtgatttc aaacagcggg
    37621 tagggcagtc tgtcgagatc gagtcgcatg atctcggcga agtggcggtc accgctgagc
    37681 agaaccagcg cggggagccg ggcgtcggcc agcagttgca gcaggcgggt gcgggcggcg
    37741 gggtggttgg cccacttctc gtgacggtgt tcgaccggaa tgacctgaat gctggagacc
    37801 acaatgaaca gctcggcgct gccggggtcg agttcccgcg ccagccactg ccactgttcg
    37861 gcgccgagga tgtcagccat ccgcccgggt ggctcgcgct ggaagcgggt gtcgagcagg
    37921 atcaccttga cccgctgtcc cggcggaccc tgaagatggc tggcgtagat gcccgcctga
    37981 cgacgccggg ggctgttggc cggttcgtcg agaaaatcga gcagcaggcg ctggctttcg
    38041 actttttgcc gatagctgcg atcgccatcg ttgcggccgt agtcatggtc atcccaggtg
    38101 ccgatgacct ggcagcaggc cagcagtttc tggtaatcgg gctggctctt ctgctgccga
    38161 tagagtttgg ccatgctgtc catctcttcg gtgtcggcat agatgatgtc gccactccag
    38221 atccacagtt gcgggcgcaa ttgcagcaca gccggccaga tcggttgtgc ctggtcggaa
    38281 cggttgcagg agccgaaggc gatgcgatcg agcggtgctt cgctggcgct tgccagtgtg
    38341 ccgcacagca gaagtcccag cagcaggaag tggcccgctc cgcgccggcg tcggtgtgac
    38401 tcaccccttt tcatgcgcgg tccagaggct gtagggatgc tggctgaggc aggcattgct
    38461 gtagcgcgga tcgtcgctga cctcgcggcc gacccaaggc ggcagttcga caagctcgtc
    38521 gacgtgttcc agttcgacct cggcgagcat cagcccgctg ttggcgccag cgaagatgtc
    38581 gacttcccac aggtgttggc catgttggag tcggtgacgc accttgtcga tcggtggcca
    38641 gaggcagagc ctgtcgagca tctcctgcgc atcggtcagc gggatcgggt attcgtactc
    38701 caggcgggca atcgattggc caggcaggct gcccttcagg gtcagccagg ccgactgccc
    38761 ggcgatgcgc acccgcagta cgcgatcatc agcaacgcag agataaccct gacgataatg
    38821 gctggcctgc ccctgcggtt gccacagtgc ggcgttgacg aggaatttgc gctcgatttc
    38881 gatggccacg atccaaggct ccgtcttggg tccgatgctg tatacaggct ggcagcgtca
    38941 gatgaagatc gcgttgcaac ggatcgaaac aggccgatcg gcctgtaggg tggagagtct
    39001 caaggagatg aagccagtca agtggcgtcc cctaggggat tcgaacccct gttcccgccg
    39061 tgaaagggcg gtgtcctgac cgctggacga aggggactct cggctgtcgg gtgtggtggt
    39121 ggagctaggc gggatcgaac cgccgacctc ttgcatgcca tgcaagcgct ctcccagctg
    39181 agctatagcc ccagcctcac ccaaaagagt cggcaattat aaggagggca tgtcatgctg
    39241 tcaaagaccc gggttcatat tttttcaggt cgggtgcggt gtgacggttt gccacgccac
    39301 ggcgctgtgc gtgcgctgcc gcccgatgga taattggcgg catggagccg aatgcaggga
    39361 ggttttcgat gtcgatcaag cattggccgg cggccgaacg gccccgcgaa aaactgttga
    39421 tgcgcggtgc tgctgcactc tccgacgccg agttgctggc gatctttctg cgcaccggcg
    39481 tgcgcggcaa gagcgcggtc gatctggcgc gcgagctgct ggtcgagttt ggtggcctgc
    39541 ggccgctgct ggaggcggat ctgcaacgtt tctgccgcgg ctccggcctt ggcgaagcca
    39601 agtatgtgca gcttcaggcc gtgctggaga tgagcagccg ccacctgcag caggggcttg
    39661 agcgtggcga tgcgctcacc tcgccggcgc agacgcggcg ttttctgagc tcgcgcctgc
    39721 gtcatctcga gcgcgaggtc ttcatggtgc tctatctcga caaccgccat cggatgatcg
    39781 ccttcgagga gctgttcaag ggctcgatca acagtgccga ggtgcatccg cgcgagctga
    39841 tcaagcgggt gctccatttc aacgcagcgg cggtgattct ggcacacaac cacccctccg
    39901 gcattgccga gccgagtcag gccgaccgcg agatcaccca gcggttgcgt gccgcactgg
    39961 cgctggtcga tgtgcgactg ctcgaccaca tggtggtggg cgacaccgag gtgatttcgc
    40021 tggccgagct ggggtgtctg taggggcggc gtccgcagac ccggtggagt caggcgattc
    40081 gcagcgggct ccactcgatc tcgatggtgg ccggcggcat cacctgcttg acatgaaaac
    40141 cggccttgcg cgccagttgc agcagggtgg catcaccggt cgggatggtg gcgatcaacc
    40201 cctgaccagc ggtggcggcc tcggcttcga tcagctccat catgcgacgg gcgacaccgc
    40261 ggcggcgggt cagcgagcgg acgcagagca gctccatgcg ccatggcgtg ctctgccggt
    40321 cgaccaccat cgcggccagc aggcggccat tgaagcgccc gccatagagt gaatgcgtct
    40381 gcaggcgctc ttcaacgaag cgctggattc tcgcctcatc ctggccggca aagccggggt
    40441 cgtcgtggta gatcttgatc agatcggcga catcctgggc cgaaggggtg acgagctgtt
    40501 cgagcaggac cggcatggtg gactccggcg aatggtcttt gacgacgaag gggcatcact
    40561 atagcgcaaa atcggctgtg cggtgcggcc ggctgcggcg ccggtcggcc ggctggttta
    40621 tactgcgcgc ctttcctctt tgtgtcgtat ccccccatct gaaacataaa ggagagcgcc
    40681 agattgaatc cacgtcg
//
LOCUS       DAORCQ010000008        40655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_481_length_40655_cov_5.991158, whole genome shotgun sequence.
ACCESSION   DAORCQ010000008 DAORCQ010000000
VERSION     DAORCQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 40655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40655
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_481_length_40655_cov_5.991158"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKI23_01475"
     CDS             complement(<1..200)
                     /locus_tag="PKI23_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN35638.1"
                     /translation="MAQYIYTMQRVSKVVPPKREILRNISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGVDKEFDGEARPLA"
     gene            complement(388..1464)
                     /locus_tag="PKI23_01480"
     CDS             complement(388..1464)
                     /locus_tag="PKI23_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004588886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNN35639.1"
                     /translation="MTLYCSGRCDLAIINGRTDHIDDSNTTAGVPPISSAHHHGHDHG
                     HHHAHQDVHNLKLAFFLNLGFALLELIGALLTNSLAVASDALHDLGDSLALGLAWFLG
                     HHAERRPDQRYSYGYRRFSLMGALINSAILLGGSLYLLLQAVPRLLDPQPVHAPGMIL
                     LALLGIAVNGAVALRLRGGRGLNSQVISWHFIEDLLGWLAVLCVSILLLFKEWPILDP
                     LLSIGFTLFILYNVHGLFKKSVALFLQGVPDEISLDEVLDAIRAIDQVADCHHTHVWS
                     MDGEHHVFSSHLVLEKDLSIDQVLALKEQVRTLVLRFGFSHSTVELEYPAEQCRMEPL
                     DHDLAPLPEHGHSHAHDQTAPNPS"
     gene            1494..3200
                     /gene="glgP"
                     /locus_tag="PKI23_01485"
     CDS             1494..3200
                     /gene="glgP"
                     /locus_tag="PKI23_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNN35640.1"
                     /translation="MPHHEFTRRPRTAYFSMEIALGQGIQTYSGGLGILAGDTLRSAA
                     DLSVPMVGVTLVSRHGYFRQQLDAEGWQSESDDGWEPERFASRLDSKVALTIAGRQVW
                     ISGWLHVLGGCIDGRLPVILLDTRLDENHPDDRAITDHLYGGDETYRLKQEMVLGFGG
                     VRMLRALGFEIRQHHMNEGHSAFLALELMLRDAYLPEELRPGEPRYDLPKVRDLCIFT
                     THTPVEAGHDKFDYGLVEATMGSLVDLPTLRQLAGEQQLNMTRLALNLSEYVNGVAKR
                     HAEVSRQNFPGYRIHAVTNGVHPATWTAPSFQRLYDHHLPGWCHEPELLSRADQITDA
                     EIWAAHEEAKAALIARIVQLTGVRFHPKLPIIGFARRMTAYKRPALLFSDLARLRAIA
                     GSYPLQIVLAGKAHPHDQEGKGLIQALHGWIKALEDEIPIAFLPNYDMELGQLLTSGS
                     DLWLNTPLRPLEASGTSGMKAAFNGVPQLSVLDGWWIEGCVEGVTGWRIGDSAEQPAN
                     GFDSEALYQKLEEVVLPLYYGYEGEQSAWIAVMKGAISKNAAYFNSHRMMRRYASEAY
                     VL"
     gene            complement(3237..5141)
                     /locus_tag="PKI23_01490"
     CDS             complement(3237..5141)
                     /locus_tag="PKI23_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN35641.1"
                     /translation="MAELLLQLSNVQLTVGHPPLIDGVDLDIQNGERIALIGRNGAGK
                     STLMKLVAGLIPPDDGRIIQGDNLNLSWLAQEVPTRLQGSVAEVVAQGFGEVNQWFDA
                     HHLLLAKPRLTATEQQELERLQGLLDAHDGWRLAQRLDALLDRMDLRADAQIENLSGG
                     FKRRVLLARALINEPNLLLLDEPTNHLDIRQIDWLERFLLDFAGSVLFISHDRSLVRR
                     LATRIVELERGRLSNWPGDYDRYLTGRMATLEEEERRNALFDKRLAAEEVWIRQGIKA
                     RRTRNEGRVRALEAMRRERAARRNRDGTVNLRVDGSQRSGQLVIEARNLSHGFAGTPL
                     IQGFSTLIQRGDKVGILGPNGCGKSTLINLLLGQISPDSGEVRHGSRLEIAYFDQLRE
                     TLDEESSLIDVVAQGRSSITINGQEKSVMGYLGDFLFSPRQVRAPVKALSGGERNRLL
                     LARLFTQPANVLVLDEPTNDLDTETLEILESLLINYPGTLLLVSHDRTFLDNVVTSLI
                     AFDADGELREYVGGYSDYLRQCPALPPQRQERVKAAPARQPSVAAAATSPAKLSYKEQ
                     RELDALPAQIDTLETEQRQLLASMNQPGFYAQDPALITSTSQRIEQIAQALEACFSRW
                     EALEQRSTGR"
     gene            complement(5232..7046)
                     /locus_tag="PKI23_01495"
     CDS             complement(5232..7046)
                     /locus_tag="PKI23_01495"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNN35642.1"
                     /translation="MSPWPPEDIRRLCLELDSVLAQQRLNILFQPIASLRGRTVMGYE
                     ALVRGPADSPLHNPLVLFGTAERCERLSELDLLCQRLAASRFLAMGLPGQLFLNMVPS
                     LLLESRHDSRHAIEQLRATGLSPQRVVLELSERYPLEDSESLRLALTPYLQAGYRVAI
                     DDLGAGYASLRLWSELRPAYVKIDQHFIRGLDDDPIKREFVRSMGEMARSAGAQVVAE
                     GVETAGEFGALAELGIELAQGYHLARPVAEPPLTLAAGLFERDLPLLRDAWRWQRGQT
                     VGELARPNPSITPDTSMDAVELMFRQRPDLNTLPVVADGRVVGVIQRITFNHLYFSRY
                     GRELYGRKPVAWFMQGEPLTVEHDLQLADVSRLLTDVVADQVEQEFIVVHEGHYLGMV
                     RVLDLLRRITDLQMRAARHANPLTLLPGNVPVYECVADLLRRQTSFAVAYCDIDHFKP
                     FNDLHGYSRGDEVIKRLAELAVQHCDSERDLIGHIGGDDFVLVLCSADWHERCERLLS
                     AFKEAALEFSSSEEQAAGGIWSEDRRGQKQFFPLLGLSIGVVLPDPARCHSHHDVAAL
                     AAKAKHQAKRRRGAAADGALYVDRRRGPDSVQTTASPM"
     gene            complement(7244..7450)
                     /locus_tag="PKI23_01500"
     CDS             complement(7244..7450)
                     /locus_tag="PKI23_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35643.1"
                     /translation="MPSTTGPRKLSLKEVEAIRRVESRGIQVDYQAITDQLLRNQHLG
                     QPEQPVFSVIYRDYYPRLQPLRGA"
     gene            7711..9717
                     /locus_tag="PKI23_01505"
     CDS             7711..9717
                     /locus_tag="PKI23_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNN35644.1"
                     /translation="MIGFCRRTAAWLLAALLLTLPPFLLAAQEPPVDVRVVIDISGSM
                     KKNDPHTLRIPAANLLIDLLPANSRAGIWLFGEQVNLLVRHAQVDSAWRELARRQVGK
                     IDSSGLFTHIGAALEQAGYDLKQPTAKGRVHLIFLTDGLVDVSKDPARNQAERQRIIE
                     KILPRYQQAGVTIHTIALSAEADHALLKQMARTTGGISATANHADELLKLFLQALEQA
                     APATELPLADNRFSVDGAIEEFTLLALHPAQADAQVRLLAPDGTAYEAQQYPVTVSWH
                     RDRGYDLVTVRKPAAGEWVLQSDPDPGNRVRVVSNLQLKVDGIPGSLAEAAPINGRFW
                     LEEAGKPLADADLLRLLTITAQLSQNERLLNSGQLASAGQPPADGIYGVSVTGVDLPG
                     SYEFLLKIDGKSFVRQYRQRIQFAPPGLDHVTEVQLPADAFGVRFEQSKDAKGGYRLE
                     VTARHPEIDLGTVEAKVMVHPPHNQGKVRDLPKQGTGRWQLELLPAELPEAGQYRLAL
                     QLRGKTRQGAPFNLYPDEIVFIEAASSAGEADAEKEVAEADDASAEEAHSNRVQMGVA
                     AGVFFLAVGGVGYFFWRRYRKSVADAQQEVSDAVKQREERRKAQATREASSARCAESA
                     VEVEADAEMGAAEEVAESGKEQVDADELDRLVDEILADEERPST"
     gene            9930..10598
                     /locus_tag="PKI23_01510"
     CDS             9930..10598
                     /locus_tag="PKI23_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNN35645.1"
                     /translation="MSEPSGERRVLDAARKLFSEQDYASVSISAIADEAGVSKANIYH
                     HFSSKSQLYYQVLKLACADATQAMQWCCEEAGSYREMLHRFVLRHLVNLLNNPADSKL
                     MIRELFEAGKNLQSELNCEAIPAQEIDCNFARLVTLLREGQQTGEFRAELNPSVLAVL
                     IIGANILFMKGSDVLSRLPDIVYPKKPEMFSAMVVDVLLNGILNRPAVAGANSSDVGQ
                     GLNS"
     gene            10598..11701
                     /locus_tag="PKI23_01515"
     CDS             10598..11701
                     /locus_tag="PKI23_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNN35646.1"
                     /translation="MKKAILLSLALASLPLLITGCANKEAEKKAEQTERPATLITVAK
                     VQKSMLQVTEKSLGRIETAAKPKVAAEVAGQVVRLHVDVGRSVKKGDLLAQIDDTAYQ
                     NELRASSAEVRRVQAQLENQERQVKRLQDLSKQNFVSKTQLDDAEAQKRVLTQQLEGV
                     SARLAIVQRNLDKTRVFAPVAGEVQRRLVSPGDFVAPGAPLFELTTHQKLQIVLPFPE
                     KTASRLKPGLEVKLSLNVEEGGVQAHGELVGVVTEVRPDIATQNLSADVIVEVDNPGD
                     WRPGASVDGVLVLEERQTLMVSDLAVVQRPAGTVVYVIKDGVAEQRQVTTGVWQKGRI
                     EIRTGIEEGDTVAEEGAYYLTNGAKVRIKESKP"
     gene            11698..14805
                     /locus_tag="PKI23_01520"
     CDS             11698..14805
                     /locus_tag="PKI23_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNN35647.1"
                     /translation="MTLPELSIKRYAMAYMLNLVIVLVGVIGLTRIGIDRWPNVDFPI
                     ISVTTTLKGANPTVIDSSVSKIIEGAVNSVTGIDYIEASSTPSVSNVVIFFKSSKDME
                     VAFNEVQSKVNTVLNQLPSEADIPIVRKIEFGAFPIMWVVLSGDRTQEQLGDYALNVV
                     KKQLEGIEGVGNIQLGGSNERTIRVNVDLERMAALGVTAQDLTRAFNNEHLQAPGGFM
                     VGENREFLIKLDQEYHSLRELNELVIGYRNDAPILLKDVATIVDGVADRRSSGFFIGQ
                     KTAGLGIVKVSNANMVAVIDRVQKVVKEQVEPLLPPGMKVTIATDDSLFVRGLISSLN
                     EHIFYGTLLAALVVLVFLHDWRSTLIVSLAIPVSLFGAVAVMYFFDFTFNSFTMLALL
                     LLIGVVVDDAIVVLENIHRHRSTLDKNPITSAISGSNEVFFAVLAASLSLVCIFGPVI
                     FMSGLVGKFFQSFAVVTTIGVLASFFVSLTLTPVLCSRFLKAEHKLGPIARFNEGWIQ
                     KSERGYERLLDFSLNHRWMVLGIATLVVASSGYFFKNIGKGFNPEVDESRFMVFFKAP
                     IGSSVSFTESKLAEIWRVLDSHPEVMNHFSGVGLMTGMQGSEGMAMVRLVPKNERKLS
                     QLEVQNLVQKDLAAIAGIRAFVVPPNPMPGGRSDPIQIVLRANNYADLVREGTSLYER
                     LQQKPELGNVDMDLQNDLPEMNLEVDRLRATSLGLSARDVATSINLMLGGADIAFYND
                     SAGTGERYDIRIKANESEIGQPLDLKKLYLRTSSGQLVRADTVAEFVPNLGPAKLSKF
                     NLKYAATYYITPKVPLAQALEMIEVETDALPLGFSTDVKGQARSFGEAINAVGFALVL
                     ALVLLYMVLASQFNGFIQPLIVMLAQPLAIVGGLFALWLFDSELVMYSMIGMVLLIGL
                     VAKNSILLVDLTNQYRENRGMGVDEALRMACPIRMRPVVMTSLTLILAMLPAAVSRGV
                     GAETNVPLALTVIGGMISSTLLTLVVVPAMYSLVENFRLRFRDQRMQRAYAAVPKKQE
                     LDAGH"
     gene            complement(14861..16759)
                     /locus_tag="PKI23_01525"
     CDS             complement(14861..16759)
                     /locus_tag="PKI23_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNN35648.1"
                     /translation="MDTQPIWDTLISGALIFDGTGTPPQRIDLAIKNGQIAAKGIDLP
                     RTAAATVIDGQGKWLMPGLLDIHTHFDLEVEVEPALTEAVRHGTTTVVMSNCSLGLAF
                     GALTSPERPEENPLIDCFARVENMPKRVLERCLDGTIDWHDSGSYLHHLEQLPLGPNV
                     APMVPHSMLRIAVMGLEGSISREPTAAELQQMCALVEQAMQQGYVGFSSDGLPLHYLA
                     NDPHRNEKIPAQHATLKEIRALAEVVRRHDRVWQATPDPEDGLKTLGIFGLTCGRLHG
                     RPLTMTATAAMDINCNQFAHRGLRLFSRLLNSRLLRGDFTFQALSVPFKVFADGVSSP
                     LLEEKPAFRELMAIDIEDVEHRQRLLNDAGYVARFRRDWRAGKEGFNLNRLRHWLKME
                     STTFSRELDDMVVERAPIALWSGQRLGEVYRRLRSYQQGDRRSAASSEEAGFFAAAPP
                     INDDADFMLHLLRQFDRALRWYVITANQRPEVLRELLFDPQTLPGFNDSGAHLTNMAY
                     FDGNLSTLKMAAQESLDQVSLAVKRLTRDPARLFRMEVGTLEMAAQADLILVDPQALL
                     DYDSDAGLTMIHREVFDHPQLVNRSDGVVDKVWIAGKLAWDGATFTPEVGKERFGRLM
                     TARAQAAA"
     gene            16990..17955
                     /locus_tag="PKI23_01530"
     CDS             16990..17955
                     /locus_tag="PKI23_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN35649.1"
                     /translation="MPYLILRLLGTALGVLLRRPLRGAAQPDWSLRTEMTVAVLRQAM
                     RYAAAHPQQWRQLESRRSQPIAVADQLERRAEQIGDMAAEWFEPHRLRQPQRVLLYLH
                     GGAYLFGSVQGFRPFLSRLAVENGIRVLAVDYRLAPEHRFPAAQQDALAAYQWLLDQG
                     HEPEQIIIAGDSAGGGLSCALLLQLKQLAQPQPRAALLFSPWVHPLATGGSMITNLDY
                     DILEADWLRDAIRLSMGELDADHPLVAPVHADLRALAPMLIIGGEAEIFRDQIIRFAQ
                     HLRDAGGEVELELWPQMMHDFQILFPELPQSKSAMARVSQFLAQL"
     gene            18005..18700
                     /locus_tag="PKI23_01535"
     CDS             18005..18700
                     /locus_tag="PKI23_01535"
                     /inference="COORDINATES: protein motif:HMM:NF022083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNN35650.1"
                     /translation="MNGNERDSTFRSLRQRARQHDAADVVFTAREQTLHCFSALAEDG
                     SPLFAVDATIDWGGDPQPASWSVSLQGQTGSTTLLRLLGNAAPVAKDDAELHARFEAF
                     FPGRLNAASRLWRLEPQAVELDGKPFGTAVQWLLPTAGWRGDRQYITDHMNEDHIEQM
                     QAMCRHFFAVAPRQVTLIALDSEGMHLRADGQVHYLPFARCCDGTREVAMETVRLSAE
                     AGAGPDHGAATAG"
     gene            complement(18712..19491)
                     /locus_tag="PKI23_01540"
     CDS             complement(18712..19491)
                     /locus_tag="PKI23_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNN35651.1"
                     /translation="MDSAAVLFSFDDGVAEITLNRPDNRNSMTPDLIAGFAEAMARLL
                     EIEAEVRCLIITGRGSSFCAGGDFSGDRVQQQLPHERLLATYRSFLQIAELKMPVIGA
                     LNGHAIGGGFGVALICDLRIANRDALYGANFARLGLHSGMSISYMLPRIVGLPRAAEL
                     LFTGRRINGAEAEQIGLVNAAVEAEQVLPRARALAREIAVCAPVAVRMMKRSLYLNAP
                     WDPQRAADFEAHCQSRTFETEDSREGITALLEKREPRFQGR"
     gene            19725..20981
                     /locus_tag="PKI23_01545"
     CDS             19725..20981
                     /locus_tag="PKI23_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNN35652.1"
                     /translation="MASSPESSTQPPSSADGAEKTRFSEFELIPPQLLRAIDEAGFEY
                     CTPIQAQSLVHTLHGLDVAGQAQTGTGKTAAFLITIFAGLINHPLDEERYSGEPRALV
                     LAPTRELALQIAKDAEQLGRYLGLRTAALVGGMDYQRQRAALQQGLIDLVVATPGRLI
                     DFIENREIFLDQIEILVLDEADRMLDMGFLPQIRRVIRQTPRKEHRQTLLFSATLNAD
                     IMQLAEQWMVDARWIEAVRSPQNAGAVKQEIYLLADHQKSTVLINLLHRFAGQRVIVF
                     ANRRDRTRHLEEKLARHGLKVGMLSGDVPQVKRLKTLEAFRAGKLDVMVATDVAGRGI
                     HIDDIALVINYDLPEEAEDYVHRVGRTGRAGATGHSISFASEHDAFELPKIEQLLGLK
                     IDAHQPEEALLQPVRPQAQRNRTATD"
     gene            21132..21950
                     /locus_tag="PKI23_01550"
     CDS             21132..21950
                     /locus_tag="PKI23_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN35653.1"
                     /translation="MLAIVKNLYDYRELMTTLAWKNIALRYKQAYLGIAWAVLKPVTL
                     MLIFTLVRSFVGIDSGSTPYPILVFAALIPWTFFQESASEGVNSVVGNTVLIRKIYFP
                     REVFPIIAVLTKLVELGINLCILAGLMLWYQMTPSIHLLWAPLIIFYTMLAALTIAFV
                     GAAINVYYRDVGQALPVLLSLLMYGSPVIYPLHLVKEKLLVERAAGDWSDAFYTLYTL
                     NPLSGIIDAFQNVVLRGLPPDLGAMTPGAILVVLLLPLSYLYFKRAESHFADVI"
     gene            21963..22478
                     /locus_tag="PKI23_01555"
     CDS             21963..22478
                     /locus_tag="PKI23_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN35654.1"
                     /translation="MPAIEVNHLTKEFRLGAVEGLKQTLLNTGARLIGRPVAERPLFK
                     ALDDVSFSIEQGEVVGIIGHNGAGKSTLLKMLAKISTPTHGEVKINGRIAPLIEVGAG
                     FVPTSPVARMSTSMARSWACQNRRLRRSSMRSSNLPRSKPSSTPRSSATAPACRSNWP
                     LPWPPASNPKF"
     gene            22751..23215
                     /locus_tag="PKI23_01560"
     CDS             22751..23215
                     /locus_tag="PKI23_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNN35655.1"
                     /translation="MSSTFSSGELELQRVSITNADGEETDAVVYGEAVTITLSFVAKS
                     AIENLAVGVGVHTPDLLYIANDDSSSSKLGPLSVPAGLFQIIYRIVCFPLYAGTYALR
                     IGAATADADRPLFYGENLCHFQVVAGAYNQSRTVGQGFFSLGGDWGILKNSE"
     gene            23259..24101
                     /locus_tag="PKI23_01565"
     CDS             23259..24101
                     /locus_tag="PKI23_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNN35656.1"
                     /translation="MSSEECGSPMISYAQNFEDVMLQRVFKEQSSGFYIDIGVWDATI
                     DSVTRHFYDQGWRGINVEPLPDNYRKILAERPRDINLNVAISSCPGSLAFHHVHNTGL
                     STSRDDYARMHAERGFEVSELVVPTRTLKEICDEHAAGVVIDFLKIDVEGAEADVIRS
                     GDWRLYRPRVVLVESVIPNSPIASFSEWEPALLGNGYVFVYFDGLNRFYLREEDLHFR
                     EAFYAPPNVFDEFVIFRIEVMKKEMSRLRERLNSAMKSMDENEKFRGSFFGKVAYKAF
                     MFFS"
     gene            24326..25576
                     /locus_tag="PKI23_01570"
     CDS             24326..25576
                     /locus_tag="PKI23_01570"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNN35657.1"
                     /translation="MISDIRAIAFYLPQYHPVKENDEWWGKGFTEWTNTAKARPLFPG
                     HYQPHIPADLGFYDLRLSEARQAQADLAREYGIHGFCYYHYWFNGRRILERPFNEVLQ
                     SGRPDFPFCLCWANENWTRVWDGGERDVLLGQNYNHEDDFLHIQSLIPAFRDPRYIRI
                     NGRPLFLVYRTELLPDPARTAEIWREVAIKAGVGDPYLVRVESFSQNISPASIGFDAA
                     VEFAPDGNDMGEWIFHHGAAGILSRMGVIPHAYAEQRIVSYPALARGMASRPVAEFTR
                     FRCVTPMWDNSARRKKNARIVIDSTPELYESWLRVLCNQTSKRFKGDERVVFINAWNE
                     WAEGNHLEPDLKWGHEYLKATRRALTEAGQTVHFSPVESNASTTHDFSEWLKSGYWKW
                     RARLGRRWVRKKELLRLLLWRWRR"
     gene            25620..26828
                     /locus_tag="PKI23_01575"
     CDS             25620..26828
                     /locus_tag="PKI23_01575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN35658.1"
                     /translation="MKSISFGSPVHILFISHDAALFGAQRMLLALLQGLDRVHFSPYL
                     LVPGKGDLVDAVHDLGIPVFEKHLEHWAPCINMMKGRGRLSYLAHMLRGLRARSWAIA
                     RLIERHGIDLIYTNTVTCIEGAVAARMMKKPHLWHIHEPVTGNSELLPVLPVSFYAPI
                     IARLSTHVVFPSRAVAADYPRLREMASIVYNGIKLPPKQNRMRARDEVAQRLKVDPLQ
                     HWVAVVGAIQPRKDHRTFLAAATNVLKYRDDVHFIIVGTGAEHLVQALRNQIETLALT
                     GRVTLAGRWEGDIAIFLAAIDVLVISSEQESFGLTAIESLAVETPVVATRCGGPAEII
                     EDGSDGFLVDVKDPPAMANAVLKLLADPAMRSAFGEAGRIRVSMRFTERQYVQGMQDA
                     LLELSRSKDV"
     gene            26877..28124
                     /locus_tag="PKI23_01580"
     CDS             26877..28124
                     /locus_tag="PKI23_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNN35659.1"
                     /translation="MNSDVRAIALYLPQFHPIPENDEWWGRGFTEWTNVAKANPLFPG
                     HYQPHVPADLGFYDLRLPEARQAQVDLAREYGIHGFCYYHYWFNGRRILERPFNEVLQ
                     SGKPDFPFCLCWANENWSRAWDGGTKKILLEQRYSEEDDRAHIESLIPALIDSRYIRI
                     DGRPLLLVYRTELLPNPARTAEIWREVARRSGVGELYLVRVENFVADIDPRNIGFDAA
                     MEFSPDNREVGQAVFSGSISRKMSRWGILPRAFHQHTVLRYPIIAASRMKRASPVYKR
                     FRCVTPMWDNTARRKKDGRILIGSTPQLYKNWLSTMVRQTKTRFEGDERVLFINAWNE
                     WAEGCHLEPDVKHGRAYLEATRQALTEATPSASLSSQESFTLKSDVSEEKKSAFRRFY
                     WGVLQKLRSLIEIIKMILRIRVS"
     gene            28131..29198
                     /locus_tag="PKI23_01585"
     CDS             28131..29198
                     /locus_tag="PKI23_01585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNN35660.1"
                     /translation="MDQARGDQQVMQPLVCIIVLNWNGRNDTLECLESIARVDYPNFE
                     VVVADNGSTDGSVEAIGRYHPEVKIIENHANLGFAEGNNGAIRHALETGADFIFLLNN
                     DTVVDPGVVTEFVRAAQQMPQAGVFGAKIYHHADSRRLWYAGGYWDSRTLSFNEYGAG
                     EIDRGQYDVLTETEWVIGCAMFIRAEVFRKVGLLEPRFFLNNEEIDFCSRAKRAGFLC
                     AYVPGARVWHKISVSFGGEDSPLKEYFSARNRLLWAKRNAALGLRLRIYLHSVSVLIR
                     RFIRPLFSVVMSQPFSLKKWWWTVCTAFLDPRNRAALMGFRDFWLRRFGNCPDSIRTL
                     TSEWSSMRVQRASSRSTGQER"
     gene            29319..30284
                     /locus_tag="PKI23_01590"
     CDS             29319..30284
                     /locus_tag="PKI23_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNN35661.1"
                     /translation="MQDHKWASLLYNQCLKHHHWQQSEGLRGRLWRWAWSQGLHRFGD
                     VAVSTNMHGRRTLVNFGHSYGLYARRFLQWNTPLIELAHQAFLARHRPIHLVDVGASV
                     GDTVRLLQANCGDEIATYLCVEGDADFFSYLKSNLEDDEKVRLVQALLSDRADEIPSL
                     VRTHPGTASAQGDQVQRAVPLDVVVEAERIEQLDVLKIDVDGFDGKVLMGSHRLLDRW
                     KPAVIFEWDPYFCQKTENSWTRHFELLVEHGYDRFLWFTKYGNFSHPMTGYDAVAVEL
                     LAQLCLSGAHDFNWHYDVVALHRESPIDPHALSMARFAKQRRSWF"
     gene            30331..31470
                     /locus_tag="PKI23_01595"
     CDS             30331..31470
                     /locus_tag="PKI23_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNN35662.1"
                     /translation="MRILYASERPPYPCFLGGAARCAHRLMQSLSEEFAVDCAAVGSA
                     EYAVTPWGHPPPEEHAALGIRSVAQPSGVIDCGYPVQLLPDFFDALESFIGRFRPDLI
                     WAQLEGAQQVLMLARRLGIPGVLYVHDAETAPNELKAMGRLGCHVVCSSEFLANKVER
                     IIGRPVQVVYPASDWYFGTLGDPQGSVLMINPVKVKGVETLLEIARRRPEVQFLLLES
                     WKLGESALHSLQAQLAALPNVRFHPRVSDMRAIYQQARLLLVPSTWEEGFGMVAVEAQ
                     SCGIPVIASARGGLPESVAEGGLLIQNHLDIDAWLQALDRILDDPDQYRQWRQAALVH
                     ARSTRFTPRDCAQRFLAACRAPLPSMGAFARGRRWLLDRMLRQRG"
     gene            31476..33422
                     /locus_tag="PKI23_01600"
     CDS             31476..33422
                     /locus_tag="PKI23_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNN35663.1"
                     /translation="MDRKKRLSLWVAVAMLAAGCSEQRRAPLFEEVTEAAGLAAYVGM
                     THGVAWGDFDGDDRPDLYVTNHLDAPLLFRNLGNGKFAEVTQSQFDAADLAGDKHGAL
                     WADFNNDGQLDLVQLTGAQQGVGAEPKRLFMNQNGRFTEVAARVGVDNPQGRTRMPLW
                     VDLDRDGRLDLFQGAEARFDALTPPFLFMQQDGRFDAAQPLPFASRSVPFCLLTELTQ
                     DAHPELLCRVVGKNKTAQLFDLASLPARELDLLPVTAFEDAAAGDFDNDGAIDLLLAR
                     KNPAGALALGQPAANLLIADLWVTAADVGKPLGFSFKASGQLDVQVRAAYAAQGLGAQ
                     QIHIGRQGEHPAGVDFALPGEMADVDGVAPHQSGEQAALYLGRQSTDRWQIEFSAATA
                     AAAAHKPQQITVMVRAATAITELTPTAEATRNEQAPQRLFMNQQGKLVEQGDKRGVNE
                     VPISAVNVVAGDFDNDMHLDLFLLGSGDVGMQQNLLLRNRGDGRFEAVPQAGGAQGSM
                     AGVGDSVTTVDHDGDGFLDLLIASGGSMGRSLGLPSAGGGYRLYHNVRNGNHWIEIDL
                     EGTRSNRDGIGARVEVTAGGVTQTRLQDGGVHERSQNHSRLHFGLGPNSQIDRLTIHW
                     PSGVVQQLSGVAADQLLRVQEVAQ"
     gene            33555..34475
                     /locus_tag="PKI23_01605"
     CDS             33555..34475
                     /locus_tag="PKI23_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNN35664.1"
                     /translation="MFFRKNKPPSHERAMQWFKANMVADQGIIVHTRERVPYPEVTGY
                     FIPTLYNWGEQALARSCTRWLLSIQLPSGAFPAPDGKPYTFDTGQIMRGLCAALGDVE
                     GAEQSLRRACDWVVAQIDAQGRLTTPSTELWSDIASDLIHTYVLPPLQQAGRVLGVPA
                     YEEAASFVLAYYKQQDGLVPFNRLSHFHAYAMEALCELGEFDLARRGMADVERCQRRD
                     GSIPAYPDVDWVCSTGMAQYAIVWYTLGNRRPADLAMRHLEKIQNNSGGFYGSYGKGA
                     KYISGAEISWAVKYFLDAWRLKMQQEGSHD"
     gene            34471..35601
                     /gene="wecB"
                     /locus_tag="PKI23_01610"
     CDS             34471..35601
                     /gene="wecB"
                     /locus_tag="PKI23_01610"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNN35665.1"
                     /translation="MKVLTVFGTRPETIKMAPVIAELAKHPTRIHNKNCLTSQHRDMV
                     APLIDLFGIKVDYNLDLMRENQTLEHITVTVLQEVSKILRAEPFDLLLVQGDTTTSMA
                     AAMAAFYCGVKVGHIEAGLRTFDKFHPYPEESNRKIIDSMADLFFAHSDRARQHLLNE
                     GIASEQIAVTGNTVIDALLDVSNRPYSFAGTPLEAVANERRRIILVTAHRRESFGGPF
                     ESICRGLAELARKYRDQVVLVYPVHRNPNVRQVVNAMLAGIDNVLLIDPLDYLPLVHL
                     MKRCHLVLTDSGGLQEEAPSLGKPVLVLRKVTERQEGVEAGTLKVIGMQADEIVRQAS
                     RLLEDEAAYQAMASRSNPYGDGTARIRIVQRILDDAGLGGTQ"
     gene            35598..36578
                     /locus_tag="PKI23_01615"
     CDS             35598..36578
                     /locus_tag="PKI23_01615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN35666.1"
                     /translation="MSVERGSPLVSIVLPTYRRPHLLPHAIRSVLAQSYPHWELIVVD
                     DNSPDHTPEVVASFTDPRIRAVRNDPNLKLPRTLNRGFALASGELLTWTSDDNLYHPQ
                     AIEKMVARLQRGDCDFVYADYWLFSELDAAGAPLDIQHDRLPDRLQLERGNHIGACFM
                     YSRALAEAVGEYDPELFLVEDYDYFIRAARQFRFAHIAEPLYYFKRDDATLYISRYCE
                     VKASDLLVRYRNGLLDGAGVVEAVVELLLRNIAGLKNPLLRGGHALLRTRSFRLTNWH
                     AKLTSHHLRRQLHGPVLALLDGYRQQQRSFGESRQALVALINALGAVSYQ"
     gene            36610..37539
                     /locus_tag="PKI23_01620"
     CDS             36610..37539
                     /locus_tag="PKI23_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNN35667.1"
                     /translation="MSFFQILSDALRGPAQARAAHRQALLRQPGIRSGIEQLQQHCPA
                     VIEADEPAPIFLLSAGWRSGSTLLQRLIMSDARVLIWGEPYDECGLVQALAGTLPPFR
                     PDWPPADYFYDGRPPGDLSGNWIANLFPALSDLRQAHRALFDTLFGVPAARAGVARWG
                     IKEVRFGIEQGLYLRWLYPRARFLFLYRHPLEAYRSYSRYGRSWYDTFPDRPVFTPTD
                     FGRHWCTLMAGFLRDAQKLDALLVRYEDLIGEQPPIDAIEAHLDIHIDRAVLTDKVGS
                     SERGGAKVRVNRLEQWLLRRAVEPVASQLGYLW"
     gene            37626..>39244
                     /gene="mutL"
                     /locus_tag="PKI23_01625"
     CDS             37626..>39244
                     /gene="mutL"
                     /locus_tag="PKI23_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNN35668.1"
                     /translation="MKPIRELPAALANQIAAGEVVERPASVLKELLENSLDAQASSID
                     VEVEAGGVRRIQVRDDGSGVPGDQLALALARHATSKIALLDDLERIATLGFRGEALAS
                     IASVSKLSFTSNPQHDSTQGWRISTEGLADGPQLLPAPHPRGSSLEVRELFFNTPARR
                     RFLRSERTEFERINELFKQIALSRFDVAFSLRHNQRLIHRLDAVTEPGSARRVEQILG
                     RDFLEQALVVDRHGDGLRLWGWIGMPTFSRSQADLQYFYVNGRTIRDKLVSHALRQAY
                     RDVLYQGRHPVCLLYLEIDPLAVDVNVHPTKHEVRFRDSRGVHDFIYSALHRAIAELQ
                     PADRIALSAAPISDRPQPGLYGAAAPLQQQPLALRPPTAAVTPNQVAESLTFYAALAQ
                     PLAEAAADEAEPPLGHALGQLHEIYILAQNRQGLVLVDMHAAHERILYERLKRSHDQA
                     PLACQPLLLPLSLPATPAEVELLDACGELLRRLGIDAEPLGPQQIVLRSLPALLAQVD
                     AAQLLRDILADLRVHGSSDRVELERNQLLAT"
     assembly_gap    39245..39344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <39345..39552
                     /gene="mutL"
                     /locus_tag="PKI23_01630"
     CDS             <39345..39552
                     /gene="mutL"
                     /locus_tag="PKI23_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253633.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNN35669.1"
                     /translation="VELERNQLLATIACHGAVRARRRLTLPEMNALLRDIEQTERNGQ
                     CNHGRPTWMQIELATVDQWFLRGR"
     gene            39702..40511
                     /locus_tag="PKI23_01635"
     CDS             39702..40511
                     /locus_tag="PKI23_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35670.1"
                     /translation="MRKLIPVALLTLLGATHHNDALAAAQRVLVTGTLGSTIEGDATQ
                     LLTNVNPWLGQRFTIDLRIDTSNSTYSHDPDPEIPGEFVNSWYGPTIHYDISIGNVAA
                     FSGVESTFTWIESGNDITVPAGVASLPPFIQVGHTYDWLDIDSSDVYLGCKSGDGHAY
                     CIGDPRDVHEGFGINISHVWDVAQHDALLDDSFPLLNGGPDFSKAVNAEFYLNFWHFS
                     QLGSSGSDIVGILGSIDSISVTNVSAVPLPQTWALLLPALGMVGVMKRRKA"
BASE COUNT         7685 a        11856 c        12813 g         8201 t
ORIGIN      
        1 gccaacggcc gtgcctcgcc gtcgaactcc ttgtcgacgc cggccatgat ccgcagcagc
       61 gtcgacttgc ccgagccgtt gagcccgagc acgccgatct tggcgcccgg aaagaacgac
      121 agcgagatgt tgcgcagaat ctcgcgcttg ggcggcacga ccttgctgac gcgctgcatg
      181 gtgtagatgt attgggccat ggggcggcgg ttctccgagc tgaattgaca aggccggggt
      241 gcggcaggcc gggacggtgc cgaaccggat cagcatggtg gctgcggcag ggcgccgttc
      301 acggcgggat tggctgcgca gtgtatccag cgcgctgccg attgcccaga tcgaccgacc
      361 gggtcggtcg gtcagtggca ggctgattca ggatggattg ggtgccgtct ggtcatgggc
      421 gtggctgtgg ccatgctcgg gcagaggcgc gaggtcatgg tcgagcggtt ccatgcggca
      481 ctgttcggcg ggatattcca gctccaccgt gctgtggctg aagccgaagc gcagcaccag
      541 tgtgcgcacc tgctccttca gtgccagcac ctggtcgatg ctgaggtctt tctccagcac
      601 cagatggctg ctgaagacat ggtgttcacc atccatcgac cagacatggg tgtgatggca
      661 atcagccacc tgatcgatgg cgcggattgc atcgagcact tcatcgagcg aaatctcgtc
      721 gggcacaccc tgcaggaaca gcgcgacgct ctttttgaac aggccatgga cgttgtacag
      781 aatgaacagg gtgaagccga tcgacaacag tggatcgagg atcggccact ccttgaacag
      841 caacaggatg ctgacgcaca gcacggcgag ccagccgagc agatcttcga tgaagtgcca
      901 ggagatcacc tggctgttga gcccgcggcc gccgcgcagc cgcagtgcca cggcgccgtt
      961 gacggcgatg ccgagcagcg ccagcaggat catccccggc gcatgcaccg gctgtggatc
     1021 gagcagtcgt ggcaccgcct gcaacagaag atagagcgag ccgccaagca ggatggcgga
     1081 gttgatcagg gcgcccatca gcgaaaagcg tcggtagcca taggagtagc gctggtcggg
     1141 acggcgctcg gcatggtggc cgagaaacca ggccagcccc agggcgaggc tgtcgcccag
     1201 atcgtgcagc gcgtcggagg caacggccag actgttggtc agcaacgcgc cgatcagctc
     1261 cagcagggcg aagccgaggt tgagaaagaa ggccagcttc aggttgtgga catcctgatg
     1321 ggcatggtgg tggccatgat catggccatg gtggtgcgcg ctgctgatgg gcggaactcc
     1381 ggcagtggtg ttgctgtcat caatatgatc ggtgcggccg tttatgatgg caaggtcgca
     1441 tcgaccgcta cagtaaagag tcaaccagac ttcgatcgaa aggatctctc gcaatgccgc
     1501 accatgaatt cacccgtcgc ccacgcactg cctatttttc gatggagatc gcgctggggc
     1561 agggcataca gacctacagt gggggcttgg gcatcctggc cggcgacacc ctgcgttcgg
     1621 ccgccgacct gtcggtgccg atggtcgggg tgacactggt gagtcgtcac ggctacttcc
     1681 gccagcagct cgatgccgag gggtggcaga gcgaaagcga tgatggctgg gagccggagc
     1741 ggttcgccag tcgcctcgac agcaaggtgg cactgaccat cgccggccgc caggtgtgga
     1801 tcagcggctg gctccatgtg ctgggtggct gcatcgatgg ccggctgccg gtgatcctgc
     1861 tcgacacccg gcttgatgag aaccacccgg atgaccgggc gatcaccgac catctgtatg
     1921 gcggcgacga gacctaccgt ctgaagcagg agatggtgct cggctttggt ggcgtgcgca
     1981 tgttgcgtgc gctcggtttc gagattcgcc agcaccacat gaacgagggc cactcggcct
     2041 ttctggcgct ggagctgatg ctgcgcgatg cctacctgcc cgaggagctg cgtccgggcg
     2101 agccgcgtta cgacctgccc aaggtgcgcg atctctgcat cttcaccacc cacaccccgg
     2161 tcgaggccgg ccacgacaag ttcgactatg gcctggtcga ggcaaccatg gggtcactgg
     2221 tcgacctgcc gacgctcagg cagctggccg gcgagcagca gctcaacatg acccggctgg
     2281 cgctcaacct cagcgagtat gtgaacgggg tggccaagcg ccatgccgag gtgtcgcggc
     2341 agaactttcc tggctatcgc attcatgcgg tgaccaatgg cgtgcatccg gcgacctgga
     2401 ccgcacccag cttccagcgg ctctacgacc accatctgcc cggctggtgt catgaacccg
     2461 agctgctgtc acgcgccgat cagatcaccg atgccgagat ctgggcggcg catgaggagg
     2521 ccaaggcggc gctgatcgcc cggatcgtac agctcaccgg cgtcaggttt caccccaagc
     2581 tgccgatcat cggctttgcc cgccgcatga ccgcctacaa gcggccggcg ctgctgttct
     2641 ccgacctcgc ccgtctgcgt gccatcgccg gcagttatcc gttgcagatc gtgctggccg
     2701 gcaaggcgca tccgcacgat caggagggca aagggctgat tcaggcgctg catggatgga
     2761 tcaaggcgct ggaggatgag attccgatcg cctttctgcc caactacgac atggagctgg
     2821 gacaactgct gacctccggc agcgacctct ggctcaacac cccgctgcgg ccgctggagg
     2881 cctccggcac cagtggcatg aaggcggcct tcaacggcgt gccacagttg agtgtgctcg
     2941 atggctggtg gatcgaaggc tgtgtcgagg gggtgaccgg ttggcgcatc ggtgacagtg
     3001 ccgagcagcc agccaacggc ttcgacagtg aggcgctcta tcagaagctg gaggaggtgg
     3061 tgttgccgct ctattacggc tatgaaggcg agcagagcgc ctggatcgcc gtgatgaagg
     3121 gggcgatcag caagaatgcg gcctatttca acagtcaccg catgatgcgc cgctatgcct
     3181 cggaggccta cgtgctctag ggttgcctca ctcccgggag tcgatcagcc accgcttcag
     3241 cgccccgtgc tgcgctgttc gagcgcctcc cagcggctga agcaggcttc cagggcctgc
     3301 gctatctgct cgatgcgctg gctggtcgat gtgatcaggg ctgggtcctg cgcatagaaa
     3361 ccgggctggt tcatcgacgc cagcagttgt cgctgctcgg tttcgagcgt gtcgatctgt
     3421 gccggcaatg catcgagttc ccgctgctcc ttgtagctca gtttggcagg gctggtggcc
     3481 gccgcagcga cggaaggctg ccgtgccggt gcggctttga cccgctcctg ccgctgtggc
     3541 ggcagggcag gacactggcg cagatagtcg ctgtaaccgc cgacatactc cctgagttcg
     3601 ccatcggcat cgaatgcgat caggctggtc accacattgt cgagaaaggt ccggtcgtgg
     3661 ctcaccagca gcagcgtgcc ggggtagttg atcagcaggc tctccagaat ctccaatgtt
     3721 tcggtgtcga ggtcgttggt gggttcatcc agcaccagca cattggccgg ttgggtgaac
     3781 aggcgcgcca gcagcagccg gttgcgctcg ccgcccgaca gcgccttgac cggcgcacgc
     3841 acctgacgcg gcgaaaagag aaaatcaccc aggtagccca tcaccgactt ctcctgcccg
     3901 ttgatggtga tgctgctgcg cccctgtgcc accacgtcga tcaggctgct ctcttcgtcg
     3961 agtgtttcgc gcaactggtc gaagtaggcg atctcgagcc ggctgccatg gcgaacctcg
     4021 ccggaatcgg ggctgatctg gccgagcagc aggttgatca gcgtgctctt gccacagccg
     4081 ttcggcccga ggatgccgac cttgtcgcca cgctggatca gggtcgaaaa gccctgtatc
     4141 agcggcgtgc cggcaaagcc atggctgaga ttgcgcgcct cgatcaccag ttggccagag
     4201 cgctggctgc catcgaccct gaggttgacc gtgccatcgc ggttgcgccg cgccgcgcgc
     4261 tcacggcgca tcgcctcgag cgcccgcacc cggccctcgt tgcgggtgcg ccgcgccttg
     4321 atgccctggc gaatccacac ctcttcggcg gccagccgtt tgtcgaacag ggcattgcgc
     4381 cgctcctcct cttcgagggt ggccatgcgg ccggtcaggt agcggtcgta gtcgcccggc
     4441 cagttgctga gccggccccg ctccagctcg acgatgcggg tggcgaggcg gcgcaccagc
     4501 gaacggtcgt gcgagatgaa gagcacgctg ccggcgaagt cgagcagaaa ccgctccagc
     4561 cagtcgatct gccggatgtc cagatggttg gtcggctcat cgagtagcag caggttgggc
     4621 tcattgatca gcgcccgcgc cagcagcacc cgccgcttga agccgccaga gaggttttcg
     4681 atctgtgcat cggcccgcag atccatgcga tcgagcagcg catcgaggcg ctgcgccagc
     4741 cgccagccat cgtgggcatc gagcagcccc tgcagccgct ccagctcctg ctgctcggtg
     4801 gcggtgagtc gcggtttggc cagcagaaga tgatgggcgt cgaaccactg gttgacctcg
     4861 ccgaacccct gcgccaccac ctcggcgacg ctgccctgca accgggtcgg cacctcctgg
     4921 gccagccagg agagattgag gttgtcgccc tggatgatgc ggccatcgtc gggtggaatc
     4981 agccccgcca ccagcttcat cagtgtcgat tttccagcac cgttgcgccc gatcagcgca
     5041 atgcgctcgc cgttctggat gtcgaggtcg actccgtcga tcagcggcgg atggccgacc
     5101 gtcagttgga cattgctcag ttgcagcagg agttcggcca tgcggggagt gtcggtctgc
     5161 tcgggtgagt ggggcggcaa ttctaatcgc tcgccactgc aacagcacga acagtcgtca
     5221 gagggcagat ttcacatggg cgatgcagtt gtctgcacgc tgtccggacc ccgccgacga
     5281 tcgacataga gggcgccgtc tgcggcagcg ccgcgacgcc gtttggcctg atgcttggcc
     5341 ttggccgcca gtgcggcgac atcatggtgc gagtggcagc gggccggatc gggcagcacg
     5401 acgccgatcg acagcccgag cagtggaaag aactgtttct gaccgcgtcg gtcttcgctc
     5461 cagatgccgc ccgcggcctg ctcttcgctg ctgctgaact ccagggcggc ctctttgaat
     5521 gcgctcagca gtcgttcaca gcgttcatgc caatctgcgc tgcaaagcac caggacgaaa
     5581 tcatcgccac cgatatggcc gatcaggtca cgttcgctgt cgcagtgctg cactgccagt
     5641 tcggccagac gcttgatgac ctcgtcgcca cggctgtagc catggaggtc attgaagggt
     5701 ttgaaatggt cgatgtcaca ataggccacg gcgaaggagg tctgtctgcg cagcagatcg
     5761 gcgacacatt cgtagaccgg gacgttgccg ggcagcagcg tcagcggatt ggcgtggcgg
     5821 gcggcacgca tctgcaggtc ggtgatgcgc cgcagcagat cgagcacgcg caccatgccc
     5881 aggtagtggc cttcatggac gacgatgaac tcctgctcga cctggtcagc gaccacatcg
     5941 gtcagcaggc ggctgacatc ggccagctgc aggtcgtgct cgaccgtcaa gggttcaccc
     6001 tgcatgaacc aggcgactgg cttgcggcca tagagttcgc gcccgtagcg actgaagtag
     6061 agatggttga acgtgatgcg ctggatcacg cccaccaccc tgccgtcagc caccaccggc
     6121 agcgtgttca gatcgggacg ctgacggaac atcagctcca cggcgtccat gctggtgtcg
     6181 ggggtgatgc tcgggttcgg tcgagccagc tcgcccaccg tctggccgcg ttgccagcgc
     6241 caggcgtcac gcaacagagg cagatcgcgc tcgaacagac cggctgccag cgtcagcggt
     6301 ggctcggcaa ccgggcgcgc caggtggtag ccctgagcca gttcgatgcc aagctcggcc
     6361 aatgcgccga attcgccggc ggtttccacc ccttcggcca ccacctgggc gccggcgctg
     6421 cgcgccatct cgcccatcga gcgcacgaat tcacgcttga tcggatcgtc gtccaggcca
     6481 cggatgaagt gctgatcgat cttgacatag gctggacgca gctcggacca cagacgcagg
     6541 ctcgcatagc ccgccccgag atcgtcgatg gcgacccgat aacccgcctg cagatagggg
     6601 gtcagggcca gacgcagact ttccgaatcc tccagcggat agcgctcgct cagctccagc
     6661 accacccgtt gtggtgacag gccggtggcc cgcaactgct cgatggcatg tcgtgaatca
     6721 tgccgagact ccagcaacag gctcggcacc atgttcagga acagttggcc gggaaggccc
     6781 atggccagaa accggctggc ggcaagccgt tggcacagca aatccagctc ggagagtcgc
     6841 tcgcaccgtt cagcagtgcc aaacaggacc agcggattgt gcagcgggct gtcggcagga
     6901 ccacggacca aggcctcgta acccatgacc gtgcgaccgc gcagcgatgc gatcggttga
     6961 aacagaatgt tgagccgctg ctgtgccagg acgctgtcga gttcgaggca gagacggcgg
     7021 atatcttcgg ggggccaggg tgacatcggg aatgagaact gcttaaccga taaaaatcag
     7081 aatgttatgc agtaattatg acgtttttat ttccacgatg gctgttgatt ggcacccctc
     7141 gattgcttca ggaggaagca gggcgagcgt ggagtgtccg gaaggcgaag cgggcatccg
     7201 gacaaggagt gacgggttga aaccgcgtgc gagtgagagt ggctcaggcg cctctcaggg
     7261 gttgcagtcg agggtaatag tcacggtaga tcaccgaaaa gaccggctgt tccggctggc
     7321 cgagatgctg gttgcgcagc aactggtcgg tgatcgcctg gtaatcgacc tggatgccgc
     7381 gcgactccac gcggcggatc gcttccacct ccttgaggga gagctttcgg ggtccggtcg
     7441 ttgacggcat ctcttctcct ccgcgatgtc gggtcaatgt ctacaggcaa cagcggcgcc
     7501 gccttggggc gtgagggtgc tgcgcttgcc agccacaaca acagaaattc ttcaactcaa
     7561 gcgtagccga actgtgcgct gttgcagatt ttttccggtt ttcgggttgg aaagcagaat
     7621 gaaaagagtc tgctaggctt gagaagagtg ctttttacag tgcgtggccg actgttcgtg
     7681 ccatcacggt gtgcaacgga gagtgaacgg atgatcggtt tctgtcgtcg gacagccgcc
     7741 tggttgctgg cggcactgct gctgacgctg ccaccatttc tgctggcagc gcaggagccg
     7801 ccagtcgatg tgcgggtggt catcgacatc tcgggcagca tgaaaaagaa cgatccgcac
     7861 actctgcgca ttccggcggc gaacctgttg atcgatctgc tgccggccaa cagccgtgcc
     7921 ggcatctggc tgtttggcga gcaggtcaac ctgctggtca ggcacgcgca ggtcgacagt
     7981 gcctggcgcg aactggcgcg ccgtcaagtc ggcaagatcg actcatccgg gctcttcacc
     8041 cacatcggcg ccgcgctgga gcaggcgggt tacgatctga agcaaccgac cgccaaggga
     8101 cgggttcatc tgatctttct gaccgatggc ctcgtcgatg tcagcaagga tccggcacgc
     8161 aatcaggccg agcggcaacg catcatcgag aagatcctgc cgcgctatca acaggcgggc
     8221 gtgacgattc acaccattgc cctgtcggcc gaggctgacc atgcgctgct caagcagatg
     8281 gcgcggacaa ccggtggcat cagcgccacg gccaaccacg ccgatgagtt gttgaagctg
     8341 tttctgcagg cgctggagca ggcggcgccc gccaccgagc tgccactggc cgacaaccgt
     8401 ttcagtgtcg acggtgccat cgaagagttc acgcttctgg cgctgcatcc ggcgcaggcc
     8461 gatgcgcagg tgcggctgct ggcgccggat ggcacggcct acgaggcgca acaatacccg
     8521 gtgacggtca gttggcaccg tgaccggggc tacgacctgg tgacggtgcg caagcccgcg
     8581 gccggggagt gggtgttgca gtccgatcct gacccgggca accgggtgcg ggtggtcagc
     8641 aatctgcaac tgaaggtcga tggcatcccg ggcagcctgg ccgaggcggc accgatcaac
     8701 ggccgattct ggctcgaaga ggcgggcaaa ccgctggccg atgccgatct gttgcgcctg
     8761 ctgaccatca ctgcccaact ctcccagaac gagcgcctgc tgaacagcgg gcaactggcg
     8821 agtgcaggtc agccgccggc cgacggcatc tatggggtca gcgtcaccgg tgttgatctg
     8881 cctggaagtt acgagtttct gctcaagatc gatggaaaaa gctttgtccg ccaatatcgg
     8941 caacgaatcc agttcgcacc gccggggctc gatcatgtga ccgaggtgca actgccggcc
     9001 gatgccttcg gcgtgcgctt cgagcagtcc aaggatgcca agggcggtta ccgattggag
     9061 gtgaccgcgc gtcatccgga gatcgacctg gggacggtcg aggccaaggt gatggttcat
     9121 ccgccgcaca atcagggcaa agtgcgcgat ttgcccaagc aggggacggg tcgctggcag
     9181 ctcgagctgc tgcccgccga actgcccgaa gccggtcagt accgtctcgc cctgcaactg
     9241 cgcggcaaga cccgacaggg tgcgcctttc aatctctatc ccgatgagat cgttttcatc
     9301 gaggcggcga gctcggcagg cgaggcggat gctgaaaaag aggttgcaga ggccgatgat
     9361 gcctcggctg aggaagcgca ctcgaatcgg gtgcagatgg gagttgctgc aggggtattt
     9421 tttctggctg tcggcggtgt gggttacttt ttctggcggc gttatcgcaa gagtgtcgct
     9481 gacgcgcagc aggaggtttc cgacgcggtc aaacagcgcg aggagcgacg caaagcccaa
     9541 gccacgcgcg aagcgtcttc ggccaggtgt gctgagtctg ccgtggaggt cgaggccgat
     9601 gccgagatgg gcgcagcaga ggaggtggcg gagtccggta aggagcaggt cgacgcggat
     9661 gaactcgaca ggctggttga tgagattctg gccgacgaag agcgtccatc cacttgactg
     9721 ttccggcgcg ctggctggaa gtttcccgcg ttccgcccgg cgatgaattt caaaagagtt
     9781 ggaaaagcga tcttgccgcc actataatcc ggaccgatcg atcggtcagt gtcttgttct
     9841 gtgcgattgt ttcttcggtc aaggaaggtc aaatcgatgg cgttggcagt gtctggctcg
     9901 tctgagcgct ccttggttcc ggattgcaga tgagtgaacc aagcggcgag cgcagggtgc
     9961 tcgatgcggc gcggaagctc ttttccgaac aggactatgc cagcgtctcg atcagcgcca
    10021 ttgcggatga ggccggcgtg agcaaggcca acatctatca ccatttcagt tcaaagagtc
    10081 agctctatta ccaggtgctg aagctggcct gtgccgatgc gacccaggcc atgcaatggt
    10141 gctgtgaaga ggcgggctcc taccgggaga tgttgcacag gttcgtgctt cgccatctgg
    10201 tcaatctgct gaacaacccc gccgactcca agctgatgat ccgcgagctg ttcgaggcag
    10261 gaaagaacct ccagtccgag ctgaattgtg aagcgattcc ggcgcaggag atcgactgca
    10321 acttcgcccg tctggtgacg ctgttgcgcg aggggcagca aaccggcgag tttcgcgccg
    10381 aactcaaccc atcggtgctg gcggtgttga tcatcggcgc caacatcctg ttcatgaaag
    10441 gcagcgacgt tctgagcaga ctgcctgaca tcgtctatcc gaaaaaaccg gagatgtttt
    10501 ccgccatggt ggtcgatgtg ctgctcaatg gtattctcaa ccgccccgct gttgccgggg
    10561 caaactcttc tgatgtgggt caaggactta actcgtaatg aaaaaagcca ttctgctatc
    10621 gcttgcgctg gcaagcctgc cgctgctgat caccggttgc gccaacaagg aggccgagaa
    10681 aaaggcggaa cagaccgagc gtccggccac cttgatcacg gtggcaaaag ttcagaagag
    10741 catgcttcag gtgaccgaga aatcactcgg tcgcatcgag actgctgcca aaccgaaagt
    10801 ggctgcggag gtggccggac aggtcgttcg tttgcatgtc gacgtgggtc gcagtgtcaa
    10861 aaagggcgat ctgctggcgc agatcgatga caccgcctat cagaacgagt tgcgtgccag
    10921 cagtgccgag gtgcgtcggg tgcaggccca gctcgagaat caggagcgcc aggtcaagcg
    10981 gctgcaggat ctgagcaagc agaatttcgt ctccaagacg cagctcgatg atgccgaggc
    11041 acagaagcgc gtgctgaccc agcaactcga gggggtttcg gcgcgattgg ccatcgtgca
    11101 gcgcaacctc gacaagaccc gggtgtttgc gccggttgcc ggcgaggtgc agcgacggct
    11161 ggtgtcgccg ggtgatttcg ttgcgccggg cgctccgctt ttcgagttga cgacccatca
    11221 gaaattgcag atcgtcctgc catttcccga aaagaccgcc agccggctga agccgggcct
    11281 cgaggtcaag ctgagcctga atgtcgagga ggggggtgtt caggcgcatg gtgaactggt
    11341 cggtgtggtg accgaagtca gacccgacat cgccacccag aacctctcgg ccgatgtgat
    11401 tgtcgaggtc gacaatcccg gcgactggcg acccggcgcc agtgtcgatg gcgtgctggt
    11461 gctggaggag cgtcagacat tgatggtttc cgacctggcg gtggtgcaaa gacccgctgg
    11521 caccgtggtc tatgtcatca aggatggggt ggccgagcag cgacaggtga ccacgggtgt
    11581 ctggcagaag ggacgcatcg agatccgcac gggcatcgaa gagggtgata ccgttgcgga
    11641 agagggcgcc tactacctga ccaacggggc caaggtacga atcaaggagt cgaagccatg
    11701 acactgcctg aactgtcgat caaacgctat gcgatggcct acatgctcaa tctggtcatc
    11761 gtgctggtgg gtgtcatcgg cctgacccgc attggcatcg accgctggcc gaatgtcgac
    11821 tttccgatca tttcggtgac caccaccctc aagggagcca atccgaccgt catcgacagc
    11881 agtgtcagca agatcatcga aggggcggtc aacagtgtga ccggcatcga ctatatcgag
    11941 gcctcctcga cccccagcgt ctccaacgtg gtgatcttct tcaagtccag caaagacatg
    12001 gaggtcgcct tcaatgaagt gcagagcaag gtcaacaccg tgctcaacca gcttccaagt
    12061 gaggcggaca ttccaattgt gcgcaagatc gagtttggcg ccttcccgat catgtgggtg
    12121 gtgctgtcgg gtgaccggac ccaggagcag ctgggtgact atgcgctgaa tgtggtcaag
    12181 aagcagctcg aaggcatcga aggcgttggc aacattcagt tgggtggttc caacgagcgg
    12241 accatccgcg tcaacgtcga tctggagcgg atggcggcac tgggggtgac cgctcaggat
    12301 ctgacccgcg ccttcaacaa tgagcacctg caggcgccgg gtggtttcat ggtcggtgag
    12361 aaccgggagt ttctgatcaa gctcgatcag gagtaccaca gcctccgtga gttgaatgag
    12421 ctggtgatcg gctaccgcaa tgatgcgccg attctgctga aggatgtggc caccatcgtc
    12481 gatggggtgg ctgaccggcg ctcgtcgggc ttcttcattg gtcagaagac ggctggactt
    12541 ggcatcgtca aggtcagcaa cgccaacatg gtggcggtca tcgacagggt gcagaaagtg
    12601 gtcaaggagc aggtcgagcc actgctgccg cctggcatga aggtgaccat cgccaccgac
    12661 gattcactct tcgtgcgtgg cctcatcagc agcctgaacg agcatatttt ttacggcacc
    12721 ttgctggccg cgctggtggt gctggttttc ctgcatgact ggcgttcgac gctgatcgta
    12781 tcgctggcca ttccggtctc cctgttcggg gccgtggcgg tgatgtactt cttcgacttc
    12841 accttcaaca gcttcacgat gttggcgctg ctgctgctga tcggtgtggt ggtcgatgac
    12901 gccatcgtgg tgctggagaa catccaccgt caccgcagca ccctcgacaa gaacccgatc
    12961 acctcggcga tctcaggcag caatgaggtg ttttttgccg tgctggcagc cagtctgtcg
    13021 ctggtctgca tttttggccc ggtgatcttc atgagtggtc tggtcggcaa gttcttccag
    13081 tcctttgccg tggtgaccac gatcggcgtg ctggcgtcgt tctttgtttc actgaccctg
    13141 acgccagtgc tctgctcccg ctttctgaaa gccgagcaca agctggggcc cattgcccgt
    13201 ttcaacgagg gctggattca gaagagtgag cgcggctacg agcggttgct ggatttctcg
    13261 ctgaaccacc gctggatggt gttgggcatc gccaccttgg tggtggcctc gagcggttat
    13321 ttcttcaaga acatcggcaa ggggttcaac ccggaagtcg atgagagtcg tttcatggtc
    13381 ttcttcaagg cgccgatcgg ttcatcggtc tcattcacag agagcaaatt ggccgagatc
    13441 tggagggtgc tcgattcaca tcccgaggtg atgaaccact tttccggtgt tggtctgatg
    13501 actggcatgc agggcagtga aggcatggca atggttcgac tggtgcccaa gaatgagcgc
    13561 aagttgtctc aactcgaagt gcagaatctg gtgcagaagg atctcgctgc catcgccggc
    13621 atccgggcgt tcgtggtgcc tcccaacccg atgcccggcg ggcgcagtga tccgattcag
    13681 atcgtcctgc gtgccaacaa ctacgctgac ctggtgcgcg aaggcacgtc gctctatgaa
    13741 cgtctgcagc agaaaccgga actgggcaat gtcgacatgg atcttcaaaa cgacctgccc
    13801 gaaatgaatc tggaagttga tcgtctgcgt gcaaccagtc tcggcttgag tgcgcgtgac
    13861 gtggccacca gcatcaacct gatgctgggt ggtgcggaca tcgctttcta caacgactca
    13921 gccggtactg gtgagcgtta cgacatccgc atcaaggcca atgagtcgga gatcggtcaa
    13981 cccctcgacc tgaagaagct ctatctgcgg accagttcgg gccagttggt cagggccgat
    14041 accgttgccg agttcgtgcc caatcttgga cccgccaagc tctccaagtt caacctgaaa
    14101 tacgcggcca cctactacat cacgcccaag gtgccgctgg cacaggcatt ggagatgatc
    14161 gaggttgaaa ccgacgccct gccactgggc ttcagcaccg atgtgaaggg acaggcgcgc
    14221 agctttggcg aggccatcaa cgcggttggt tttgcgctgg tcctggcgct ggtgctgctc
    14281 tacatggtgc tggccagcca gttcaatggc ttcatccagc cgctgatcgt gatgttggcg
    14341 cagccgctgg cgatcgtcgg tgggctgttt gcgctgtggc tgttcgattc ggaactggtg
    14401 atgtactcga tgatcggcat ggtgttgctg atcgggctgg tggccaagaa ctcgatcctg
    14461 ctggtcgatc tgaccaacca gtaccgcgag aatcggggta tgggggttga tgaggcgctg
    14521 cggatggcct gcccgatccg gatgcggccg gtggtgatga cctcgctgac gctgattctg
    14581 gcgatgttgc cggcagcggt gagccgtggg gttggcgccg agaccaacgt gccgctggca
    14641 ctgacggtga tcggcggcat gatcagctcc acgctgctga cgctggtggt ggtgcccgcg
    14701 atgtattcgc tggtcgagaa cttcaggctg cggttccgtg accagcggat gcagcgtgcc
    14761 tatgccgccg tgccgaagaa gcaggagctg gatgcaggcc actgaccgaa gccggcggca
    14821 gaaaacgggc gggctggttt caacccgccc gctgtgatcg tcaggcggcc gcctgagcgc
    14881 gggcggtcat cagcctgccg aagcgctcct tgcccacctc cggggtgaag gttgccccgt
    14941 cccacgccag ttttccggcg atccacacct tgtcgaccac accatcggag cggttgacca
    15001 gttgcggatg gtcgaagacc tcgcggtgga tcatcgtcag cccggcatcg ctgtcatagt
    15061 cgagcagtgc ctgcgggtcg accagtatca ggtcggcctg ggcggccatc tccagcgtgc
    15121 caacctccat gcggaacagt cgggccggat cccgcgtcag ccgtttgacc gccagactca
    15181 cttggtcgag tgactcctgc gcggccatct tcagcgtgct gaggttgcca tcgaagtagg
    15241 ccatgttggt cagatgggcg ccgctgtcgt tgaagccggg caatgtctgc ggatcgaaca
    15301 gcagctcacg cagcacctct ggtcgttggt tggcagtgat gacataccag cgcagggcgc
    15361 ggtcgaactg ccgcagcagg tgcagcatga agtcggcgtc gtcgttgatc ggtggcgcag
    15421 cggcaaagaa cccggcctct tcgctgctgg ctgcgctgcg tcggtcaccc tgctgatagc
    15481 tgcgcaaccg gcggtagacc tcgcccagcc gctgcccgct ccagagcgca atgggcgcac
    15541 gctcgacgac catgtcatcc agttcgcggc tgaaggtggt cgactccatc ttcagccagt
    15601 ggcgcaaccg gttcaggttg aaaccctcct tgccggcgcg ccagtcgcgg cggaagcgcg
    15661 cgacatagcc ggcgtcgttc agcagccgct ggcgatgctc gacatcctcg atatcgatcg
    15721 ccatcagctc gcgaaaggcg ggcttctctt ccagcagtgg cgaactgacg ccgtcggcaa
    15781 agaccttgaa ggggaccgac agcgcctgaa aggtgaagtc gccgcgcagc agtctggagt
    15841 tgagcagccg gctgaagagg cgcagtccgc ggtgcgcgaa ctggttgcag ttgatgtcca
    15901 tggccgcggt cgcggtcatc gtcagcggtc ggccgtgcag gcggccgcag gtcagaccga
    15961 agatgcccag tgtcttgagg ccatcctcgg ggtcgggggt ggcctgccag acccgatcgt
    16021 ggcgccgcac cacctcggcc aatgcacgga tctccttcaa ggtggcgtgc tgggccggaa
    16081 tcttttcgtt gcggtgcggg tcgttggcca gatagtgcag cggcaggcca tcggatgaaa
    16141 agccgacata accctgctgc atcgcctgtt cgaccagggc gcacatctgt tgcagttcgg
    16201 ccgcggtcgg ctcccggctg atcgaaccct ccagccccat caccgcgatg cgcagcatgc
    16261 tgtgcggcac catcggtgcg acattgggcc ccagtggcaa ctgttcgaga tggtgcagat
    16321 agctgccgct gtcatgccag tcgatggtgc catccaggca cctttcgagc acccgcttgg
    16381 gcatgttctc gacccgggcg aagcagtcga tcagcggatt ttcctcgggt cgctcggggc
    16441 tggtcagggc gccgaaggca aggccaaggc tgcaattgct catcaccacg gtggtggtgc
    16501 cgtggcggac ggcctcggtc agcgccggct ccacctcgac ttcgaggtcg aagtgggtgt
    16561 ggatgtcgag cagccccggc atcagccact tgccctggcc atcgatcacg gtcgcggccg
    16621 ctgtcctggg cagatcgatg cccttggcgg cgatttgccc attcttgatc gccagatcga
    16681 tccgctgcgg cggggtgccg gtgccatcga agatcagcgc accgctgatc agggtgtccc
    16741 agatgggttg agtgtccatg gccattcctt gcagggttgt tattgtggaa ggaaagactg
    16801 cattcgcatt ttcggccgag ttggagcata gcggagcgga ggttgccatc caagtgtttc
    16861 ggggtgtgca gccacgccgc tggtgcaggt gccgccagac aaagcggccg ttctgttcac
    16921 tggccacagg gcccgctgtt cgataggctg catcctttgg gcttgaagaa taaaaaccgg
    16981 agaaacgcga tgccgtatct gattctgcga ctgttgggca cggcattggg cgtgctgctg
    17041 cggcggccac tgcggggggc ggcgcagccg gactggtcac tgcggaccga gatgacggtg
    17101 gcggtgttgc ggcaggcgat gcgctatgcc gccgcccatc cgcaacagtg gcggcaactg
    17161 gagtcgcgcc gaagccagcc gatcgcggtg gctgaccaac tggagcgccg cgccgagcag
    17221 atcggtgaca tggccgccga atggttcgaa ccgcatcggc tcaggcagcc gcagcgggtg
    17281 ctgctctacc tgcatggtgg tgcctatctg tttggttcgg tgcaggggtt tcgccctttt
    17341 ctttcccgtc tcgcggtcga aaatggcatt cgggtgttgg cggtcgatta ccgtctggcg
    17401 ccggagcacc gttttcccgc agcacagcag gatgccctgg ccgcctatca gtggctgctc
    17461 gatcaaggcc atgagccgga gcagatcatc attgccggcg attcggccgg cggtgggctc
    17521 agctgcgcac tgctgctgca actgaagcaa ctggcccaac cccagccgcg cgcggcactg
    17581 ctgttctcgc cttgggtcca cccgttggcc accggcggta gcatgatcac caatctcgat
    17641 tacgacatcc tcgaagccga ctggctgcgt gacgccattc gcctgtcgat gggagagctg
    17701 gatgccgacc atcccctggt ggcaccagtg catgctgacc tcagggcgct ggcgccgatg
    17761 ttgatcatcg gtggcgaggc cgagattttt cgcgaccaga tcatccgctt tgcccagcat
    17821 ctgcgcgatg ccgggggcga ggtcgagctc gaactctggc cacagatgat gcatgacttc
    17881 cagattctgt tccccgaact gccccagtcg aagtcggcca tggcgcgggt cagccagttt
    17941 ctggcgcagc tgtgaatcgt gcctgtccca ctccgattct caccccttca caaggagcac
    18001 accaatgaat ggcaatgagc gtgattccac ctttcgttcg ttgcgccagc gcgcacgcca
    18061 gcacgatgca gccgatgtcg tctttacggc acgggagcag acccttcact gcttcagcgc
    18121 ccttgcggag gatggatcgc cgctctttgc ggtggatgcg acgatcgact ggggtggcga
    18181 tccgcaaccg gccagttggt cggtgtcgtt gcagggtcag acagggtcga caacactgct
    18241 caggttgttg ggcaatgctg caccggttgc gaaggatgac gccgaactgc atgcccgctt
    18301 cgaagcgttc tttccgggtc ggttgaatgc cgccagtcgg ctctggcggc tcgagcccca
    18361 ggcggtggag ctggatggaa aaccgtttgg cacggccgtg caatggctgc tgccgacggc
    18421 aggctggcgg ggcgaccggc aatacatcac cgaccacatg aacgaagacc acatcgagca
    18481 gatgcaggcc atgtgccgcc acttcttcgc tgtggctccg cggcaggtga cgctgatcgc
    18541 gctcgactca gagggcatgc atctgcgtgc cgatgggcag gtccactatc tgccgttcgc
    18601 cagatgctgc gacggcaccc gtgaagtggc gatggagaca gtgcgactgt cggccgaagc
    18661 cggggccgga cctgaccatg gcgcggccac cgccggttga gtcgctgccg ctcagcgccc
    18721 ctgaaagcgc ggttcgcgct tctcgagcag cgcggtgatg ccctccctgg aatcttcggt
    18781 ctcaaaggtg cgtgactggc agtgggcctc gaaatcggcg gcccgttgcg gatcccaggg
    18841 ggcgttcaga tagagcgacc gcttcatcat ccgcaccgcg accggcgcac agacggcgat
    18901 ctcacgggcc agcgcacggg cgcgcggcag cacctgttcg gcctcgacgg cggcattgac
    18961 caggccgatc tgttcggcct cggcgccatt gatccgcctg ccggtgaaga gcagctccgc
    19021 cgcgcggggc agtccgacga ttctcggcag catgtagctg atcgacatgc ccgaatgcag
    19081 gccgagccgc gcaaagttgg cgccatagag tgcatcgcgg ttggcaatgc gcagatcgca
    19141 gatcagcgcg accccgaagc cgccgccaat ggcgtggcca ttgagtgcgc cgatcaccgg
    19201 catcttcagt tcggcaatct gcaaaaagga gcggtaggtt gccagcaacc gctcatgtgg
    19261 caactgctgc tgcacccggt cgccgctgaa gtcgccccca gcacagaaac tgctgccgcg
    19321 cccggtgatg atcaggcagc gcacctcggc ttcgatctcc agcagtcgtg ccatggcctc
    19381 agcgaatccg gcgatcagat cgggggtcat gctgttgcgg ttgtcggggc ggttcagggt
    19441 gatctcggcc accccatcgt cgaaggaaaa cagaacggcg gcggaatcca tggcgaattc
    19501 gatctccctg tttttatgtt gtgagcgatc ctgccggcca gaatgcacag tggatcggtg
    19561 ccggccgttg cagaatagcc cgatatcgaa caaatcgaaa ccggcccacg gtctgtctca
    19621 tcgccatcac atctctgcgg aatgacgaca gggtttgcta caatcggcgc acattgcttt
    19681 tttcttttcc gtttgatcgc atcgagcatc gacctttgac atccatggca tcttccccgg
    19741 aatcatccac ccagcccccg tcctcggccg atggtgccga aaaaacccgc ttcagcgagt
    19801 tcgagctgat tcccccccaa ctgctgcgcg ccatcgatga ggcggggttc gaatactgca
    19861 cgccgattca ggcgcagtcg ctggtccata ccctgcacgg gctggatgtg gccggtcagg
    19921 cccagaccgg caccggcaag acggcggcct ttctgatcac catctttgcc gggttgatca
    19981 accatccgct cgatgaagag cgctacagcg gcgagccgcg cgcactggtg ttggcgccga
    20041 cgcgagagct ggcgttgcag attgccaagg atgccgagca actgggcaga taccttggac
    20101 ttcggaccgc cgcgctggtg ggtggaatgg attaccagcg acagcgcgct gccctgcagc
    20161 aggggctgat cgatctggtg gtggccaccc cgggacggct gatcgacttc atcgagaatc
    20221 gggagatttt tctcgatcag atcgagatcc tggtgctcga tgaggccgac cgcatgctcg
    20281 acatgggctt tctgccgcag attcgccgcg tcatccgcca gacgccacgc aaggagcatc
    20341 gccagacgct gctgttcagc gccaccctga acgccgacat catgcagctt gccgagcagt
    20401 ggatggtcga tgcccgctgg atcgaggccg tgcgcagtcc gcagaacgcg ggtgcggtca
    20461 agcaggagat ctacctgctg gccgaccatc agaagagcac cgtgctgatc aacctgctgc
    20521 accgctttgc cgggcagcgc gtcatcgtct tcgccaaccg gcgtgaccgc acccgccatc
    20581 tggaggagaa gctggcacgc cacggcctga aggtggggat gctgtcgggc gatgtgccgc
    20641 aggtcaagcg gctgaagacg ctcgaagcct tccgggccgg caagctcgac gtgatggtgg
    20701 ccaccgatgt ggctggacgc ggcattcaca tcgacgacat cgcgctggtc atcaactacg
    20761 atctgcctga agaggccgag gactacgtcc accgcgtcgg ccgtaccgga cgggccggcg
    20821 ccaccggcca ctccatcagc ttcgccagcg agcatgacgc cttcgaactg cccaagatcg
    20881 aacagctgct tggcctgaag atcgatgcac atcagcctga agaggccttg ctgcagccag
    20941 tgcggcccca ggcgcagcgc aaccggaccg ccaccgactg atcgggcggg ttgggccaag
    21001 ggcctgtggc aggccacagg cccaactggc gcccagcgcg gtttcacgca accgcaagca
    21061 attttcgact attcttgaag ttgctgccga atggtcggca ccgctttacg catggatttc
    21121 aagcacgcgc tatgctcgcc atcgtcaaaa acctctacga ctaccgcgaa ttgatgacca
    21181 cgctggcgtg gaagaacatc gcgctgcgct acaagcaggc ctatctcggc atcgcctggg
    21241 cggtgctgaa gccggtgacg ctgatgctga tcttcacgct ggtcagaagc ttcgtcggca
    21301 tcgacagcgg cagcactccc tatccgatcc tggtgtttgc cgcgctgatt ccctggacct
    21361 tctttcagga gtccgcctcc gaaggggtca acagtgtcgt tggcaatacc gtgctgatca
    21421 gaaagatcta ctttccgcgc gaggtctttc cgatcatcgc cgtgctcacc aagctggtcg
    21481 aattgggcat caacctctgc atcctggccg ggttgatgct ctggtatcag atgacgccga
    21541 gcatccatct gctgtgggcg ccgctgatta tcttctatac catgctggcc gcactgacca
    21601 tcgccttcgt cggggctgcg atcaacgtct actaccggga tgtcggtcag gcattgccgg
    21661 tgctgctctc gctgctgatg tatggctcac cggtgatcta tccactgcac ctggtcaagg
    21721 agaagctgct ggtagagcgg gccgccggcg actggtccga cgcgttctac accctctaca
    21781 ccctcaaccc gctctccggc atcatcgatg cgtttcagaa tgtcgtgctg cgtgggttgc
    21841 caccggatct gggcgcgatg acccccggcg ccatcctggt cgtcctgctg ctgccgctga
    21901 gctaccttta cttcaagcgc gccgaaagcc atttcgccga cgtgatctga gcgctcctcc
    21961 ctatgccagc cattgaagtc aatcatctca ccaaggagtt ccgtctcggc gccgtcgagg
    22021 ggctcaagca gaccctgctc aacaccggcg cacgcctgat cggccgaccg gtggcggagc
    22081 ggccactgtt caaggcgctg gatgacgtca gcttctcgat cgaacagggt gaagtggtcg
    22141 gcatcatcgg tcacaacggc gctggcaagt ccacgctatt gaaaatgctg gcgaaaatca
    22201 gcacacccac ccatggcgag gtcaagatca atgggcgcat tgcgccgctg atcgaggtgg
    22261 gtgccggttt cgtcccgact tcaccggtcg cgagaatgtc tacctcaatg gcgcgatcct
    22321 gggcatgtca aaacaggaga ttgcgcagaa gttcgatgag atcgtcgaat ttgccgagat
    22381 cgaagccttc atcgacaccc cggtcaagcg ctacagctcc ggcatgcagg tcaaactggc
    22441 ctttgccgtg gccaccagca tcgaatccga aattctgatc gtcgatgagg tgctggcggt
    22501 gggtgatctg gccttccagc gcaaatgctt cgaccggatg gaagagctga tcaagaacca
    22561 gggcagaacg gtgctgctgg tcagccacaa tattcgtcag gtcgagcggc tctgttcccg
    22621 ggtgatcctg atggaccacg gcagagcgat ccatgatggc gatccggaag ttgcctgcaa
    22681 cctgttctat gagcaaaacg acaggaaggt caaggaagaa gctgccagac gggtctcacg
    22741 gggtggcggc atgagttcga ccttctcctc tggagagctc gaactgcagc gagtgtcgat
    22801 caccaatgcc gacggagagg aaaccgatgc ggtggtttac ggagaagcag tcacgatcac
    22861 gctctcgttc gttgcaaaga gcgccatcga aaatctcgcg gttggtgtgg gtgtgcacac
    22921 accggatctt ctctatatag ccaatgatga cagcagttca agtaaattgg gtccgctctc
    22981 tgtgcctgct ggcttgtttc agatcattta tcgaattgtt tgctttcctt tgtacgcagg
    23041 gacatacgcg ctcagaatcg gagctgccac ggccgatgct gatcgaccgc tcttttatgg
    23101 tgaaaatctg tgccattttc aggtcgtggc aggggcatat aatcagagtc gaacggtggg
    23161 tcagggattt ttttctctgg gaggtgactg gggaattctg aaaaattctg aatagtcttg
    23221 cgcatgaagt tgcaagtctt ggagagagga tttccggaat gtcaagtgaa gaatgtgggt
    23281 cgcctatgat cagctatgcg caaaatttcg aagatgtcat gctgcagagg gttttcaagg
    23341 agcagagtag cggcttctat atcgatatcg gcgtttggga tgcaacgatt gattcggtga
    23401 cgcgccattt ctacgatcaa ggttggcgcg gtatcaatgt tgagccgttg ccagataatt
    23461 atcggaaaat tcttgccgag agaccgcgtg acatcaatct gaacgtggcc atttccagtt
    23521 gtcctgggtc acttgcattt catcatgttc acaacacagg actttccacc agcagagatg
    23581 actatgcgcg aatgcatgcg gagagggggt ttgaagtctc tgagcttgtt gttccgacaa
    23641 ggacgctgaa agaaatctgt gacgagcatg cggctggggt cgtcattgat tttctcaaga
    23701 tcgacgtcga gggcgccgaa gctgatgtaa tcagaagtgg cgattggcgg ttataccggc
    23761 cccgtgtggt tcttgtcgaa tcagtgatac caaactcacc gattgcatca ttctcggaat
    23821 gggagccagc gcttttggga aatggctatg tttttgtcta ttttgatggg cttaatcgtt
    23881 tttatttgcg tgaagaggat ctgcattttc gagaggcgtt ttacgctccg ccgaatgtgt
    23941 ttgatgagtt tgtcattttc cggattgagg ttatgaaaaa agagatgtca aggttgagag
    24001 agcgcttgaa ttcggcaatg aaatccatgg atgagaatga aaaattcaga ggttcttttt
    24061 tcggaaaagt ggcttacaag gcattcatgt ttttttcatg atttttttct tgaaaatatc
    24121 tgcctgacag gatgtttcag aatatggttt gaggaggcat tgatttagtc gtccccgtaa
    24181 aggagggcat tcaggcgatt gttgttgctg gattctcgtc ttcgcatgaa tggcgggcaa
    24241 gagtcaatca gtgtttcctt ttttcagtgg tttattaaaa taattttgga gtgtaatttt
    24301 ttatgcgggg gctagaaaga ttgccgtgat cagtgacatc agagcgattg ccttctattt
    24361 accgcaatac catccagtaa aagagaatga cgaatggtgg gggaaggggt tcactgaatg
    24421 gaccaataca gccaaggcaa gaccactctt tcctggtcat tatcagccgc atattccggc
    24481 tgatctgggt ttttatgatc ttcgcttatc ggaggctcgc caggcgcagg cagatttagc
    24541 cagagaatat ggcattcatg gcttctgcta ctaccactac tggttcaatg gtcgtcgaat
    24601 cctggagcgg cctttcaatg aagtgttgca aagtggcagg ccggattttc ctttctgtct
    24661 ttgctgggcc aatgaaaatt ggactcgtgt atgggatggt ggagagcgcg atgtcctgct
    24721 cggacagaat tacaatcatg aagatgattt tctgcatatt cagagcctta ttcccgcatt
    24781 ccgtgatcca agatatatcc gaatcaatgg tcgaccgctt ttcttggtat atcgcacaga
    24841 attgttgcct gatccagcgc gtactgctga aatctggcgt gaagttgcga tcaaggccgg
    24901 ggtcggagat ccatacctgg tgcgtgtaga aagtttttct caaaacattt caccggcgtc
    24961 cattggattt gatgctgccg tggagttcgc gcctgatggg aatgacatgg gagagtggat
    25021 ctttcaccat ggcgctgctg gcattctgag tcgcatgggg gtcattccac atgcctacgc
    25081 ggagcaacgg attgtcagtt atccggcgct cgccagagga atggcatctc gtcctgtcgc
    25141 tgagttcacg cggtttcgtt gcgtaacgcc gatgtgggac aacagcgctc gtcgaaagaa
    25201 aaacgcacgg atcgtgattg attccactcc ggagctttat gaaagctggc tgagagttct
    25261 ttgcaatcaa acatccaaga gattcaaagg cgatgaaagg gttgttttca tcaacgcatg
    25321 gaatgagtgg gctgaaggaa atcatttgga gcctgacctg aaatggggcc atgagtatct
    25381 gaaggcaacg cgacgtgcac tgactgaggc tggccagaca gttcattttt ctcctgtcga
    25441 atcgaatgcg tccaccactc atgatttctc tgagtggttg aagagtgggt attggaaatg
    25501 gcgagcccgt cttggcaggc gttgggtcag gaagaaggag cttctgcgtc ttctgttgtg
    25561 gcgatggcgc agatgaattt tttttgcagg ctccattttc gctgcaaggt gatttattca
    25621 tgaaatccat ctcattcggc agtccagtac atattctttt tatttcgcat gatgctgcgt
    25681 tgtttggagc gcagcgcatg ttgctggccc tgctgcaagg gttggatcgg gtgcattttt
    25741 caccttatct gctggtgcct ggaaaaggag atttggtcga tgccgtacac gacctgggca
    25801 tccccgtttt tgaaaagcat ttggaacatt gggcaccctg tatcaacatg atgaaaggcc
    25861 gtggtcgttt gtcgtatctc gcacatatgc tgcgtggtct tcgtgcgaga tcctgggcca
    25921 ttgcgcggtt gatcgagcgc catggcattg atttgatcta caccaacact gtcacttgca
    25981 tcgaaggggc ggttgcggcg cgaatgatga aaaaacccca tctctggcat attcatgagc
    26041 cggtcacggg taacagcgaa ttgctgccgg tgttgccggt atctttctac gcgcccatca
    26101 tcgcgcggtt gtcgacccat gtggtgtttc cttcccgtgc ggtggcagca gattatccac
    26161 ggttgcgtga gatggcatcg atcgtctaca acggcatcaa attgccaccg aaacagaatc
    26221 ggatgcgggc tcgcgatgaa gttgcccaga ggttgaaagt cgatcctttg cagcactggg
    26281 ttgccgtcgt gggggccatc cagccacgca aggatcaccg tacatttctg gcggcggcga
    26341 cgaatgttct gaagtatcga gatgatgtgc atttcatcat tgtcgggact ggcgcagagc
    26401 acttggtgca ggcgttgcgc aatcagatcg agacgctggc tctgacgggc agggtcacct
    26461 tggccgggag atgggagggc gacattgcca tttttcttgc ggcaatcgat gtgttggtga
    26521 tctcttcgga acaggagtct tttggcttga ccgcaatcga gtcactggcg gttgaaactc
    26581 cagtggttgc gacgcgttgc ggcggacccg ccgagatcat tgaagatggt tcagatgggt
    26641 ttctcgtcga tgtcaaggat ccaccggcca tggcgaatgc tgtgctgaag ttgcttgccg
    26701 atccagccat gcggagtgcg ttcggcgaag ctggaaggat tcgggtgtcg atgcgcttca
    26761 ccgagcgtca gtatgtccaa gggatgcagg atgcacttct ggagttgagc aggagtaaag
    26821 atgtttgatc acgggttttc gaaaaatccg attcgactgc aaagagttgg agtttcatga
    26881 acagtgatgt cagggcaata gcgctctatt tgccacaatt tcacccgatt ccagagaatg
    26941 acgaatggtg gggcagggga ttcaccgaat ggaccaatgt ggccaaggca aacccgcttt
    27001 tcccgggtca ctatcaacca catgtcccgg ccgatctggg tttttatgat cttcgcttgc
    27061 cagaggcgcg tcaggctcag gtggatttgg ccagagagta tggcattcat ggcttctgtt
    27121 actatcacta ctggttcaat ggtcgtcgca ttctggaacg gcctttcaat gaagtgctgc
    27181 aaagtggcaa gccggacttt cctttctgtc tctgctgggc gaatgagaac tggtcgcgcg
    27241 cgtgggatgg aggaacgaaa aagattctcc ttgagcagcg ctattcagaa gaggatgacc
    27301 gtgcccatat agaaagtctc atcccagcgc ttatcgattc tcgttatatt cggatcgatg
    27361 ggcggccatt gcttctcgtt tatcgaacag agcttttgcc gaaccctgcc agaacagcgg
    27421 aaatttggcg ggaggttgca aggcgctccg gagttggaga gttgtatctg gtccgggttg
    27481 aaaatttcgt tgctgatatt gatccaagga atatcggttt cgatgccgcc atggagtttt
    27541 ctccggacaa tcgagaggtg ggacaggctg ttttttctgg ttcaatctcc agaaagatga
    27601 gcagatgggg aattcttcca agagcttttc atcagcatac ggtgttgcgc tatccgatca
    27661 ttgctgcatc acgcatgaag agagccagcc ctgtatacaa acgattccgt tgcgtcaccc
    27721 cgatgtggga caacacggcg agacgaaaaa aagatgggcg tatcttgatc ggatcaacgc
    27781 cgcagctcta caagaattgg ctgagcacca tggtgcgaca gacgaagacc agattcgaag
    27841 gtgatgagag agttcttttc atcaatgcat ggaatgagtg ggcagagggc tgccacctcg
    27901 aacccgatgt caagcacggg cgtgcctatc tggaggcaac ccgacaggcg ctgacggaag
    27961 caactccttc cgcttctctg tccagtcagg aatctttcac gctcaagtct gacgtgagcg
    28021 aagaaaaaaa atctgctttt cgtcggtttt attggggagt gttgcaaaaa ttgcgaagct
    28081 tgatcgagat catcaagatg attttaagaa ttagagtgag ctgatcatca atggatcagg
    28141 caagaggtga tcagcaagtc atgcaaccgt tggtttgcat catcgttctg aattggaatg
    28201 gccgaaatga tacgctggaa tgcttggagt ccattgcaag ggtcgactat ccgaattttg
    28261 aggtcgttgt ggcagacaac ggatcgaccg atggttctgt cgaagccatc ggtcgatatc
    28321 atccagaagt taaaataatc gagaaccatg ccaatcttgg ttttgcagaa ggcaacaatg
    28381 gcgccattcg tcatgcgctc gaaacaggtg cagatttcat ttttttgctc aataacgata
    28441 ctgtcgttga tccgggcgtt gttacggaat ttgtccgggc tgcgcaacag atgccgcaag
    28501 ccggagtttt cggggcaaag atttaccatc atgccgacag ccgcaggttg tggtatgccg
    28561 gtggatattg ggatagccgg acgttgagtt tcaatgaata tggcgccggt gagattgacc
    28621 gtgggcagta cgatgtgctg accgaaaccg agtgggtgat tggttgtgcc atgttcatcc
    28681 gcgccgaagt gttcaggaag gttggcctgc tcgaaccaag attctttctc aacaatgaag
    28741 agatcgattt ttgcagtcgg gccaagcgtg ccggcttttt gtgtgcctat gttccaggtg
    28801 cgcgggtgtg gcacaagatt tcggtctcct ttggcggaga agattcacca ctgaaggaat
    28861 atttcagtgc cagaaaccga ttgttgtggg cgaagcgcaa tgcagcattg gggttgcggt
    28921 taagaatcta tttgcacagt gtcagcgttc tgattcggcg tttcattcgt ccgctgttta
    28981 gtgtagtgat gagtcagcca ttctcgttga aaaaatggtg gtggacggtt tgtaccgcat
    29041 tcctcgatcc tagaaaccgt gccgccttga tgggttttcg tgatttctgg ctgagacggt
    29101 tcggtaactg cccggacagt atcaggacgc tcaccagcga atggtcatcg atgcgtgtac
    29161 agcgggcttc gtctcgatcg acaggtcagg agcgctgatt gttctggaag agttgcagaa
    29221 tctttttgaa agaatcttcg atccaaagag ttgagttcaa ttgagctgta cgatgacggc
    29281 agaggtttcg tttttgaatc gttcgaacaa ggctggccat gcaagatcat aaatgggcgt
    29341 cattgctcta caaccagtgt ctgaaacacc atcattggca gcagtccgaa ggtttgcgtg
    29401 gccgcttgtg gcgctgggcc tggagccagg gattgcatcg atttggcgat gtggcagttt
    29461 ccaccaacat gcatggtcga cggactttgg tcaattttgg ccatagctat ggtctgtatg
    29521 cccgtcggtt tttgcagtgg aacacgccac tcatcgaact ggcacaccag gcttttcttg
    29581 cccggcatcg ccccattcat ctggtggatg taggtgccag tgtgggtgac accgtccggt
    29641 tgttgcaagc caactgtggt gacgagatcg cgacctatct ttgcgttgaa ggtgatgccg
    29701 attttttttc ttatctgaaa agcaatctgg aggacgatga gaaggttcgt ctggttcagg
    29761 cgctgctttc cgatcgcgca gacgagattc cttcactggt gcgcacccac cccggaactg
    29821 ccagtgcgca gggcgatcaa gtacagaggg ccgtgccact cgatgtcgtt gtggaggcag
    29881 aaagaataga gcaactcgac gtgctgaaaa tcgacgtcga cggttttgat ggcaaggtgc
    29941 tgatgggcag ccacaggttg ctggaccgat ggaagcctgc cgtgatcttt gagtgggatc
    30001 cctacttctg ccagaaaacg gaaaacagct ggactcggca cttcgagttg ttggtcgagc
    30061 atggctatga ccgctttttg tggttcacca aatatggcaa tttcagccat ccgatgaccg
    30121 gctatgatgc ggtggccgtc gaattgctgg cgcaactctg tctttccggt gcccatgatt
    30181 tcaactggca ctacgatgtg gttgcattgc accgagaatc accgatcgac ccgcacgcgt
    30241 tgtcaatggc acgttttgcc aagcagcgca ggtcatggtt ctgaacgatt gcaccatgtt
    30301 cgattgcgtt gctggcactg aaaaaagctg atgcgcatcc tctacgccag cgaacgccca
    30361 ccttatccct gttttctggg cggtgcggcc cgttgtgccc atcggttgat gcagtcgttg
    30421 agcgaggagt ttgctgtcga ctgcgctgcg gtgggttcgg cggagtatgc ggtgaccccc
    30481 tgggggcacc caccgcctga ggagcatgca gcgctcggca ttcgatctgt ggcgcagccg
    30541 agtggcgtga tcgactgcgg ctatccggtt cagctgttgc ccgatttctt cgatgcgctg
    30601 gaatcgttca tcggccggtt tcggcccgat ctgatctggg cacagttgga aggcgcgcag
    30661 caggtgctga tgctggcccg gcgcttgggc atccctggcg tgctctacgt gcatgatgcc
    30721 gaaacggcgc cgaacgagct gaaagccatg ggacgacttg gctgccatgt ggtttgcagc
    30781 agtgaattcc tggcgaacaa ggtggaaagg atcatcgggc ggccggtgca ggtggtctat
    30841 cctgcgtcgg actggtattt cggcaccctg ggagacccgc agggctcggt gttgatgatc
    30901 aatccggtca aggtcaaggg tgtcgagacc ctgctggaaa tcgcgcgccg gcggcccgaa
    30961 gtgcagtttt tattgctcga atcctggaaa ctcggcgagt cggcactgca ctcgctgcag
    31021 gcgcaactgg cggccttgcc gaatgtgcgc tttcacccgc gggtgtcgga catgcgtgcc
    31081 atctaccagc aggcacgcct gctgctggtg ccttcgacct gggaggaggg gttcggcatg
    31141 gtcgcggtcg aggcgcagtc ctgcggcatt ccggtgattg ccagcgcgcg tggcggtctg
    31201 ccggagtcgg tcgcagaggg gggcttgctg attcagaacc atctcgacat cgatgcctgg
    31261 ctgcaggccc tcgatcggat tctcgatgat cccgaccagt accggcagtg gcggcaggcg
    31321 gccctggtcc atgcccggtc gaccaggttc accccgcgcg actgtgccca gcgctttctg
    31381 gccgcctgtc gggcgccgct gccgagcatg ggggccttcg cgcgcggcag acgctggctg
    31441 ctcgaccgaa tgttacggca acgaggctga atggcatgga cagaaaaaag aggctttctc
    31501 tgtgggttgc ggtggccatg ctggcggctg gctgttccga acagcgccgg gcgccactgt
    31561 tcgaagaggt caccgaagcc gccggtcttg ctgcctacgt cggcatgacc cacggtgtgg
    31621 cctggggcga cttcgatggc gatgaccggc ccgatctcta tgtgaccaac cacctcgatg
    31681 cgccgctgct gtttcgcaat ctgggcaacg gcaagttcgc cgaggtgacc cagagccagt
    31741 tcgacgccgc cgatctggca ggtgacaagc atggcgcgct gtgggccgat ttcaacaacg
    31801 atggccaact cgacctggtg cagttgactg gcgcccagca gggcgtcggg gccgagccca
    31861 agcggctgtt catgaatcag aatggccggt tcaccgaggt ggcggcccgg gtcggggtgg
    31921 acaatccgca agggcgcacc cgcatgccgc tgtgggtcga tctggatcgc gatggccggc
    31981 ttgatctgtt tcagggtgcc gaggcccgtt tcgatgcgct gacgccgccc ttcctcttca
    32041 tgcagcagga tggccggttt gatgcggcac agccgctgcc gtttgcgtcg cgctcggtgc
    32101 cgttctgcct gctcaccgaa ctgacgcagg atgcccaccc ggagctgctc tgccgggtgg
    32161 tcggcaagaa caaaactgca caactgttcg accttgccag cctgccggcg cgtgaactcg
    32221 atctgctgcc ggtgaccgcc ttcgaggatg ccgcagccgg tgacttcgac aacgatggcg
    32281 ccatcgacct gttgctggcg cgcaagaatc cggccggcgc tctggcattg ggccagccgg
    32341 ccgccaatct gctgattgcc gatctctggg tgacggccgc cgatgtcggc aagccgctgg
    32401 gtttcagctt caaggcatcc ggccagctcg acgtgcaggt cagggccgcc tacgccgcgc
    32461 agggcctcgg tgcgcagcag atccatatcg gccggcaggg cgaacatccg gccggagtcg
    32521 atttcgcgct gccgggcgag atggccgatg tcgatggtgt ggcgccgcat cagtctggtg
    32581 agcaggcggc cctctatctg ggtcgccaat cgaccgaccg ctggcagatc gagttctctg
    32641 cggccacagc ggctgcggca gcgcacaagc cacagcagat caccgtgatg gttcgcgcag
    32701 caacggccat caccgagctg acgccgacgg ccgaggccac ccgcaatgag caggcgccgc
    32761 agcggctgtt catgaaccag cagggcaagc tggtcgagca gggtgacaaa cgcggtgtca
    32821 acgaggtgcc gatctccgcg gtcaacgtgg tggccggtga tttcgacaac gacatgcatc
    32881 tcgatctgtt cctgctgggc tcgggtgacg ttggcatgca acagaatctg ctgttgcgca
    32941 accgtggcga tggccggttc gaagccgtcc cgcaggcagg tggcgcgcag ggttccatgg
    33001 ccggtgttgg cgacagtgtc accacggtcg accatgacgg cgacggcttt ctcgacctgc
    33061 tgatcgccag cggcggcagc atgggccgca gcctcggtct gccctcggct ggcggtggct
    33121 accggctcta ccataatgtg agaaacggca accactggat tgaaatcgat ctcgaaggca
    33181 cccgctccaa ccgcgatggc atcggtgcac gggtcgaagt caccgccggc ggtgtgaccc
    33241 agacgcggct gcaggatggc ggcgtccatg agcgcagcca gaaccacagc cggctccact
    33301 ttggtctggg tcccaacagt cagatcgacc ggcttaccat ccattggccg agcggtgtgg
    33361 tgcagcagtt gagcggcgtg gccgcggacc aactgctgcg ggtgcaggag gttgcccagt
    33421 gacccagagg cggcactgat tcggcatggc ccgattttct tctctattct tccaatacga
    33481 atcagcgcca atcatctcga tggatgccaa ccgtttcgta gtcaaagcca acagtggatt
    33541 ccaggaattt cgacatgttt tttcgcaaga acaaaccacc cagccatgag cgggccatgc
    33601 agtggttcaa ggcgaacatg gtggccgatc agggcatcat cgtgcacacc agggagcggg
    33661 tgccctatcc cgaggtgacc ggttatttca tccccaccct ctacaattgg ggggagcagg
    33721 cactggcgcg cagctgcacc cgctggctgc tgtcgatcca gttgccgtcg ggggcctttc
    33781 cggcgcccga tggcaagccc tacaccttcg acaccgggca gatcatgcgc gggctgtgcg
    33841 ctgcgctcgg tgatgtcgag ggcgccgaac agtcgctgcg ccgcgcctgt gactgggtgg
    33901 tggcgcagat cgatgcgcag gggcggctca ccacgccgtc gaccgagctg tggagcgaca
    33961 tcgccagcga cctgattcac acctatgtgc tgccacccct gcagcaggcg ggcagagtgc
    34021 tcggggtgcc ggcctatgaa gaggccgcca gcttcgtgct cgcctactac aaacagcagg
    34081 atggactggt ccccttcaac cgcctttcgc actttcatgc ctatgcgatg gaggcgctgt
    34141 gcgaactggg cgagttcgac ctggcgcgac ggggcatggc cgacgtggaa cgctgccagc
    34201 ggcgcgacgg cagcattccg gcctatcctg acgtcgactg ggtctgctcg accggcatgg
    34261 cacaatatgc catcgtctgg tacacgctgg gcaaccggcg tccggcagac ctcgccatgc
    34321 gccatctcga gaaaatccag aacaattcgg gcggcttcta cggcagttat ggcaaagggg
    34381 ccaagtacat ctccggcgcc gagatcagtt gggcggtgaa atatttcctc gatgcctggc
    34441 ggctgaagat gcagcaggag ggcagccatg attaaggtgc tgaccgtctt tggcacccgc
    34501 ccggagacca tcaagatggc gccggtgatc gccgagctgg cgaagcatcc gacgcgcatc
    34561 cacaacaaaa actgcctcac cagtcagcac cgggacatgg tggcgccgct gatcgacctg
    34621 ttcggcatca aggtcgatta caacctcgac ctgatgcgcg agaaccagac gctcgaacac
    34681 atcaccgtca ccgtgctgca ggaggtgagc aagatcctgc gtgccgagcc attcgacctg
    34741 ctgctggtgc agggcgacac caccacctcg atggcggcgg cgatggcggc gttctactgt
    34801 ggcgtcaagg tggggcacat cgaggccggc ctgcgcacct ttgacaaatt ccatccctat
    34861 cccgaggagt cgaaccgcaa gatcatcgac tcgatggccg atctcttctt cgcccacagc
    34921 gaccgcgccc gccagcatct gctcaacgag ggcatcgcca gcgagcagat cgcggtcacc
    34981 ggcaacaccg tgatcgatgc actgctcgat gtctccaacc gcccctacag ctttgccggc
    35041 acgccgctcg aagccgtggc gaacgagcgc cggcgcatca ttctggtgac cgcccaccgc
    35101 cgcgagagtt tcggtggccc gttcgaatcg atctgtcgcg gtctggccga gctggcgcgc
    35161 aagtatcgtg accaggtcgt gctggtctac ccggtgcacc gcaatcccaa tgtgcggcag
    35221 gtggtgaacg ccatgctggc cggcatcgac aatgtgctgc tgatcgatcc gctcgactac
    35281 ctgccgctgg tccacctgat gaagcggtgc cacctggtgc tgaccgactc cggcggcctg
    35341 caggaggaag cccccagcct cggcaagccg gtgctggtgc tgcgcaaggt gaccgagcgg
    35401 caggaggggg tcgaggctgg caccctcaag gtgatcggca tgcaggccga tgagatcgtc
    35461 cgccaggcct cgcgcctgct cgaagacgag gccgcctacc aggcgatggc ctcgcgcagc
    35521 aacccctatg gcgacggcac ggcacgcatc aggatcgtcc agcgcatcct cgacgatgcc
    35581 ggattgggcg gcacgcaatg agtgtggagc gcgggtcgcc gctggtcagc atcgtgctgc
    35641 ccacctaccg gcggccgcat ctgttgcccc acgccatccg cagcgtgctg gcgcagagct
    35701 acccgcactg ggagctgatc gtcgtcgatg acaactcgcc cgaccacacc cccgaggtgg
    35761 tggcgagctt caccgatcca cgcattcgtg ccgtgcgcaa cgaccccaac ctcaagctgc
    35821 cgcgcacgct gaaccgcggc tttgcgctgg ccagtggcga gctgctcacc tggacctcgg
    35881 acgacaacct ctaccacccg caggcgatcg agaagatggt ggcccgcctg caacgtggcg
    35941 actgcgattt cgtctatgcc gactactggc tcttctccga gctggatgcg gcaggcgcgc
    36001 cgctcgacat tcagcacgac cgcctccccg atcgactgca actggagcgg ggcaaccaca
    36061 tcggcgcctg cttcatgtac agccgggcgc tggccgaagc ggtgggagag tatgaccccg
    36121 agctgtttct ggtcgaggac tacgactact tcatccgcgc cgcccggcag ttccgcttcg
    36181 cccacatcgc cgagccgctc tactacttca agcgtgacga cgccaccctc tacatctcgc
    36241 gctactgcga ggtcaaggcc tccgatctgc tggtgcgcta caggaacggc ctgctcgatg
    36301 gcgccggcgt ggtcgaggca gtggtggaac tgctgctgcg caacatcgcc ggcctgaaga
    36361 acccgctgct gcgcggcggc catgcgctgc tgcgcacacg ctcgttcagg ctcaccaact
    36421 ggcacgcaaa actgaccagt caccacctgc ggcggcaact gcacggaccc gtgctggcgc
    36481 tgctcgacgg ctaccgccag cagcagcgca gctttggcga gagccggcag gcgctggtgg
    36541 cgctgatcaa cgctctgggt gcggtcagct accagtgatg acagcgccaa cgcaagtcag
    36601 gaagccccga tgtccttctt tcagattctg agtgacgcac tgcgcggccc ggcccaggcg
    36661 cgtgcggcgc accggcaggc gctgttgcgc cagcccggca tccgttccgg catcgagcag
    36721 ttgcagcagc actgcccggc agtgatcgaa gccgatgaac cggcaccgat ctttctgctc
    36781 tcggccggct ggcgctccgg ttcgacgctg ctgcagcggc tgatcatgtc cgacgcccgg
    36841 gtgctgatct ggggcgaacc ctatgacgaa tgcggactgg tgcaggcact ggccggcaca
    36901 ctgccgccgt tccggcccga ctggccgccg gccgactact tctacgatgg tcgccccccc
    36961 ggcgacctgt cgggcaactg gatcgccaac ctgttcccgg cactgagtga cctgcgccag
    37021 gcgcatcgtg ccctgttcga caccctgttc ggtgtgccgg cagcacgcgc cggggtggcg
    37081 cgctggggca tcaaggaggt gcgcttcggc atcgagcagg ggctctatct gcgctggctc
    37141 tacccgcggg cgcgctttct gttcctctac cgccacccgc tcgaagccta ccgctcctac
    37201 agccgctatg gccgcagctg gtacgacacc ttccctgacc gaccagtctt caccccgacc
    37261 gacttcggcc gccactggtg cacgctgatg gcaggcttcc tgcgcgatgc gcagaagctg
    37321 gacgccctgc tggtgcgcta tgaagatctg atcggcgagc agccgccgat cgacgccatc
    37381 gaggcccacc tcgacatcca catcgaccgc gccgtgctga ccgacaaggt cggcagctcg
    37441 gagcgtggtg gagccaaggt gcgggtcaac cggctggagc agtggctgct caggcgtgcg
    37501 gtggagccgg tggcgtcgca actgggctat ctctggtagg cgctggtagg cgaacggctc
    37561 ccgcaacccg gctgaattgg ccgataattg gcgcctctca ctcagagtgc cccactttgc
    37621 cgccgatgaa accgattcgc gaactgcccg ccgcactcgc caaccagatt gccgccggcg
    37681 aggtggtcga acgccccgcc tcggtgctga aggagctgct cgaaaacagc ctggatgcgc
    37741 aggccagctc gatcgacgtg gaggtcgagg ccggcggtgt caggcggatt caggtgcgtg
    37801 atgacggcag cggggtgccg ggcgaccagc tcgcgctggc gctggcgcgc catgccacca
    37861 gcaagatcgc cctgctcgat gacctggagc gcatcgccac cctcggcttt cgcggcgagg
    37921 cactcgccag catcgcctcg gtgtcgaagc tgagcttcac ctccaacccg cagcacgaca
    37981 gcacccaggg ctggcgcatc agcaccgagg ggctggccga cggcccgcag ctgctgccgg
    38041 caccccatcc gcgcggcagc agcctcgaag tgcgcgagct cttcttcaac accccggcac
    38101 ggcgccgctt tctgcgcagc gagcgcaccg agttcgagcg tatcaacgag ctgttcaagc
    38161 agatcgccct cagccgcttc gatgtcgcct tctcactgcg gcacaaccag cggctgatcc
    38221 atcggctcga cgccgtcacc gaaccgggtt cggcgcgccg ggtcgagcag attctggggc
    38281 gcgattttct cgaacaggcg ctcgtcgtcg accgccacgg cgatggcctg cggttgtggg
    38341 gctggatcgg catgccgacc ttttcgcgca gccaggccga cttgcagtac ttctatgtca
    38401 atggccggac catccgcgac aagctggtca gccacgcatt gcggcaggcc tatcgcgatg
    38461 tgctctacca gggccgccat ccggtctgtc tgctctacct cgagatcgat ccgctggcgg
    38521 tcgatgtcaa tgtccatccg accaagcacg aagtgcgctt ccgcgacagt cgcggcgtgc
    38581 atgacttcat ctactcggca ctgcaccgcg ccattgccga actgcaaccg gccgatcgca
    38641 tcgcgctgtc ggccgcgccg atcagtgacc ggccgcagcc ggggctgtat ggcgcggcgg
    38701 cgccgctgca gcaacagccg ctggcgctgc gtccgcccac tgcggcggtg acaccgaacc
    38761 aggtggcgga gagcctgacc ttctacgccg cactggcgca gccactggcc gaagccgcag
    38821 cggacgaggc agagccaccg ctcggccatg cgctggggca gttgcatgag atctacattc
    38881 tggcgcagaa ccggcagggg ctggtgctgg tcgacatgca tgccgcccat gaacgcatcc
    38941 tctatgagcg gctgaagcgc agccatgatc aggcgccgct cgcctgccag ccgctgctgc
    39001 tgccgctgtc gctgccggcc acgccggccg aggtcgaact gctcgacgcc tgcggcgaac
    39061 tgctgcggcg gctcggcatc gacgccgagc cactggggcc acagcagatc gtcctgcgca
    39121 gcctgccggc actgctggcc caggtcgatg cggcccaact gctgcgtgac atcctcgccg
    39181 acctgcgcgt ccatggcagc agcgaccggg tcgaactgga gcgcaaccag ctgctcgcca
    39241 ccatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtcga actggagcgc
    39361 aaccagctgc tcgccaccat cgcctgccac ggcgcggtgc gggcccggcg gcggctgacc
    39421 ctgccggaga tgaacgcgct gctgcgcgac atcgagcaga ccgaacgcaa cggccagtgc
    39481 aaccatggcc gccccacctg gatgcagatc gaactggcga cggtcgatca gtggtttctg
    39541 cgcgggcgtt gagcagaccg gaattcgctg agcagccagc gcaacgcagt gttacataca
    39601 aggcggtggt tctttttttt ttgcgatgtt ataaaggcgc cgttcgggca aaacccgaat
    39661 ctaccccccc acacatcgat cttcacggag gagatgcagt catgcgaaaa ctgattccgg
    39721 ttgccttgct taccctgctg ggcgccactc accacaacga tgcgcttgcc gccgcccagc
    39781 gtgtgctggt cacgggcacc ctgggctcca ccatcgaggg cgatgccacg cagctcctca
    39841 ccaatgtcaa cccctggctt ggtcaacgct tcaccatcga tctgcggatc gacacttcca
    39901 actcgaccta cagccatgat cccgatccgg aaattcctgg cgagtttgtg aacagttggt
    39961 acggccccac cattcactac gacatcagta tcggcaatgt cgcggcattt tccggggtcg
    40021 agagcacgtt cacctggatc gaatccggca atgacatcac tgttccagcc ggtgtcgcat
    40081 cgttgccgcc tttcatccag gtcgggcaca cctatgactg gctcgacatc gacagcagcg
    40141 atgtctacct tggctgcaaa tcgggcgacg gccacgctta ttgcattggt gatccgcggg
    40201 atgttcacga agggttcggg atcaatatca gccacgtctg ggatgtcgcc cagcacgatg
    40261 cgctgctcga tgactctttt ccgctgctga acggcggacc cgatttcagc aaggctgtta
    40321 acgcggagtt ctacctcaac ttctggcact tcagtcagct aggcagttcg ggcagcgaca
    40381 tcgtggggat cctgggcagc atcgacagca tcagtgtcac caacgtgagt gccgttccgc
    40441 tgccgcaaac ctgggcgctg ctgctcccgg cactcggcat ggtcggcgtg atgaagcgcc
    40501 gcaaggcgtg agccgctgac cgctcccggg cacagcggca gcgggccgtt gggcaccggt
    40561 agccggtgga aggtgacgca gccatgacgc tgcggttcag acgaaagcgg gggaggcgcc
    40621 tgctcgacgg gcgtggactg tctgcgcttt cgccc
//
LOCUS       DAORCQ010000009        39655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_505_length_39655_cov_5.446692, whole genome shotgun sequence.
ACCESSION   DAORCQ010000009 DAORCQ010000000
VERSION     DAORCQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 39655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39655
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_505_length_39655_cov_5.446692"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKI23_01640"
     CDS             complement(<1..674)
                     /locus_tag="PKI23_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35671.1"
                     /translation="MGVADFAGQMIDAAYVTILPGLVIETVVLFRIGFDDDGYADRQW
                     DLPLEQLLHYAEPVAITDQTNLRLSSLNHCPLPWLQTHLWEPDLQREHNVLVQIHEAI
                     SRNRLGLAFADEPSRPLSSATFAQHANSIGTAGFDTQAIEQRHQQEIHALRKAHQQAL
                     DELSEEIDRYARSLEEELTNNLVLKKQLHNQQVETKRLSPANRPHQAEPQEQAPHLGV
                     AQAGNTT"
     gene            1130..1624
                     /locus_tag="PKI23_01645"
     CDS             1130..1624
                     /locus_tag="PKI23_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35672.1"
                     /translation="MKMIPKRFAMALLMVASAVFSLNSHAATIDLGKLGVGDTELDIS
                     IAFGSIADTWKFEVTETGKLVSGFAGIFGIASLSADISGTPFASVPSPLPLLSPVLLG
                     PSIVLSPGMYSISVTGTTGTGFFPIGSYIGAVGLTAVPLPAPVLLLGSAMLGLVGFAR
                     RRRA"
     gene            1913..2560
                     /locus_tag="PKI23_01650"
     CDS             1913..2560
                     /locus_tag="PKI23_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNN35673.1"
                     /translation="MVRFRDRRHAGEVLAQRLAPWIGRTGVVLALPRGGVPLAAVVAP
                     RLRMPLDLIIPRKIGHPHNPEYAVAAVVESGARAINSVEVAGLDPGWFEQAVARAQQE
                     ALRRRRCYLGERSPCALEGKAVILVDDGIATGLTMEAAIQDAKARGAQELVVAVPVVP
                     ADTARRLARMVSQVVAVEIAEHYLGAVGAYYDDFSQLSDQDVIELLKQAAPAAPS"
     gene            2632..3666
                     /locus_tag="PKI23_01655"
     CDS             2632..3666
                     /locus_tag="PKI23_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35674.1"
                     /translation="MTSTLTLAEKVALLRDPHTHALPAQSVIEVVETRMSWLFLTPER
                     VYKLKKPLREPLFDFSTLARRRLNCETELRLNRRLAAEVYLDVVRLTLSSAGLCRIDG
                     EGQTLDYLVMMRRLPRARSLDQLILAGAIPPDAVEMLVRRLGDFYRQAEKSPQGATGY
                     VAHFRERIEHNQQALIAGDYPLSRQWIEQLHGRLLAYIDQHGETLQARSRQRRIVEGH
                     GDLRPEHVYLIDGQPLVIDCLEFSRELRWLDPIDELSLLDLECLRLGEPDFGRTLLAE
                     YQAIEQDPVPDHLVDFYHGYRALLWSRLAIRHLDQPQRKPGDGAKWRERARFYLQQAA
                     RVLRQPEAFG"
     gene            complement(3709..4698)
                     /locus_tag="PKI23_01660"
     CDS             complement(3709..4698)
                     /locus_tag="PKI23_01660"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNN35675.1"
                     /translation="MTNPLSIFDLDSNSSLAFATASALGAPLSAWEERQFEDGEHKAR
                     PLESVRGHHVVLLQSLYSDAAHSVNDRLVRELFFVAALKDAGATEVTVVAPYLAYARK
                     DRRSKPRDPVTHRYVAQLFEAVGSDRLLTIDVHNLAAYQNAFRIPTEHLEAKSLFVDH
                     FATLLEGNACILSPDVGGVKRAEALRRSLSRRLGRELNSAFMQKWRSRGELSGERLVG
                     DVAGQEVVIIDDLIASGATLLRTIEAAERAGARRIHAAATHALWAGKSHALLDHPALT
                     TLVVTDTLPLLQRANPVLQQRVELLSVAPLLAEAIGRIHRRESLTELWLDAET"
     gene            4836..5114
                     /locus_tag="PKI23_01665"
     CDS             4836..5114
                     /locus_tag="PKI23_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35676.1"
                     /translation="MKTMHKYEVPMTDQPFHVDLPHDAQVRWVEFLVPNKKVFMWVEV
                     DISRQGPKQRRTFHLYRSGDGIPVSYHYVGTAVDQYIPETYHLFEKVD"
     gene            5146..5547
                     /locus_tag="PKI23_01670"
     CDS             5146..5547
                     /locus_tag="PKI23_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35677.1"
                     /translation="MNRFWSESVKAPLPGHSTRRRLVLLALLGLLLAVAAPRQHAEWV
                     NSLAHEQSGQSSANDQEMAANHHCGHHGTPAGGSGLGCGCLHGLCGGLTALPGHQALS
                     ARVRIANFSPALQRTVPLPAHGRPPFRPPIS"
     gene            5622..7322
                     /locus_tag="PKI23_01675"
     CDS             5622..7322
                     /locus_tag="PKI23_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008109641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HNN35678.1"
                     /translation="MSSTKSTPAGFDWRRRRLVQGIGVGLAAGVCGLAPRFGQADPGA
                     EFTASAVASATPRELRGTQFDLTIAETPVNFTGHPRLATTINGALPAPTLRWREGDTV
                     TLRVYNRLAEESSIHWHGILLPYQMDGVPGISFPGIPPGGTFTYRFKVAQSGTYWYHS
                     HSGMQEQRGLYGAIIIDPIEGERIQADRDLVLLLSDWSDEEPMQLLKRLKVQSDYYNF
                     NQPTLADFVRDLSSAGWSAAIERRQMWQQMRMNPTDLADISAHTYRYLINGMTPADNW
                     TGLFRPGERLRLRLINGSAMTFYDLRIPGLPLTVVQVDGQNVEPVTVDELRFGPGETY
                     DVIVAPRDDVYTLFAQSMDRSGFARATLATRPGLTAPVPALDPVEWLSMTDMMGDMHG
                     GRPAHHARSEHGPGTDMRVDRARSNLDDPGVGLRNNGRRVLTLADLHSLEGVLDRRMA
                     DREIELHLTGNMERYSWSFDGVEYGQSTPVHFRHGERLRVILHNDTMMTHPMHLHGMW
                     SELESPAGAVQLRRHTFAVQPAQRISFLVTADALGRWAWHCHLMFHMDSGMFREVVVA
                     "
     gene            7319..8275
                     /locus_tag="PKI23_01680"
     CDS             7319..8275
                     /locus_tag="PKI23_01680"
                     /inference="COORDINATES: protein motif:HMM:NF017119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HNN35679.1"
                     /translation="MNPLSNEKKSAFHPAGWLKPLLLTAVLALTRQAAAETAGHAHPH
                     AIEEPPAKTIDHSRMDHGSMDHGSMDHGSGDSAREGRDPDAYSNGYRLDSGPYLLEGR
                     QHSHMADSHRFSVLRIDRLERADGRDHTGTAYDAQAWWGGSYDRLWLKAEGEHSSGTL
                     QEARSELLWSHAVAPFWDRQLGLRHDSGAGPERQWLALGLHGLAPYWFELDVAAYLGS
                     GGRSALRLGSEYELLLTQRAILQPRVEFNLYGQDDPARGLGSGLAEGVLGLRLRYEFS
                     RQLAPYLGVEWSGKFGQSADLAHSAGDATSEWRWVGGVRFWF"
     gene            8404..10659
                     /locus_tag="PKI23_01685"
     CDS             8404..10659
                     /locus_tag="PKI23_01685"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNN35680.1"
                     /translation="MCGMSVKPDSPHRSRHAGQEYRFCGARCKERFDADPQRYLHPVA
                     LPAEAMPLDVEYGCPMHPEVSQIGPGSCPKCGMALEPLLPTLDEGENPELTDFRRRFW
                     WTLPSSLLVAVISMSGELLQPLLGSMQGWVELLLATPVVAWAGGPFFVRGIQSVRQRS
                     LNMWTLISLGTGAAYLYSLTALLLPGLFPASFQMHGRVALYFEAAAVIISLTLFGQLL
                     ELKARAQTGAAIKALLGLAPKSARRLRSDGGEEEVALTRVQRGDLLRVRPGEKVPVDG
                     LVVEGESAVDESMLTGEPIPVSKRVGDRLIGATLNGGGALVMRAEKVGIETVLSQIVQ
                     MVAQAQRSRAPMQRLADQVAGYFVLGVMAVALLTLLGWGLFGPQPSWIHGLINAVAVL
                     IIACPCALGLATPMSVMVATGRGATQGVLFRDAAAIELMGKVDTLIVDKTGTLTEGRP
                     VFHQAVACAGWSESELLRIAASLDQGSEHPLAASIVAEARRRDLPLSKPEQFESSSGI
                     GVRGQVDGQPVVLGNGALMSEAAIDWQPLAQQAEALRVEGATVIFVGVAGRLAGLIAV
                     IDPIKGTTAEALRALRAGGLRIIMASGDGLTTARAVGRALGIDEVHGEVKPQQKAELV
                     ARLQAEGQIVAMAGDGINDAPALAQANVGIAMGSGTDVAMSSAHCTLVKGDLRGIETA
                     RRLSLATLGNMRQNLLFAFVYNALGVPIAAGLLYPFTGLLLSPMIAAAAMSFSSVSVV
                     SNALRLKRARI"
     gene            complement(10656..12269)
                     /locus_tag="PKI23_01690"
     CDS             complement(10656..12269)
                     /locus_tag="PKI23_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNN35681.1"
                     /translation="MSRTVDSLFLKYGPAYRWLATATVISGTLTTALSTTIVNVVIPD
                     IMGIFGVGQDKGQWMSTAFLAANTASMLVNAWCVHAYGQRKTFIAAMLFFIFGSILGG
                     LGQDINTLILARVIQGAAAGLLQPLTMQMMFRVFPEDQRGNAMGYFGIGVILAPAFGP
                     TLGGVLADNFNWRAVFFVAPTVGILAIAMAAVFLPDREDEGPARRFDWTGLALLCTFL
                     LTVLTALSNGQREGWYSDYIVTLFSVGFVSAAAFLWWELNSDRPLLQLRVYSYPGFAS
                     AAGVSIIYGIGLFGSTYIFPLFVQLQLGYTPTLSGLMLMPAGLVMGMLFLPMGRLSDH
                     VRPHYMILAGFVIFALSSLLLYDADVDTTFWTMASMIMLGRIGLTFIMPAVSVGGMGS
                     LPPHLMGQGSGAINFTRQLGGAFGVNLLSVYMSARTDLYADFLAATQIPDNSTTQALI
                     RSMAQLYAQSGVSEAHQNAGAVQYLAQVVAAKASMFAFRDAFIVVGIVFLLGIIPALV
                     LGRFCKPRGQRIPMDGEEADAELPAGSTH"
     gene            complement(12253..13395)
                     /locus_tag="PKI23_01695"
     CDS             complement(12253..13395)
                     /locus_tag="PKI23_01695"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNN35682.1"
                     /translation="MNANLGQTLQLEASAPKKPSLMKKPIVRAALTIALLGALIFFGQ
                     WIYHRSTHVYEIDARLRSTLYIVSSELPGRLESFPLDEGMTVNEGQMVAKLDSTRAEL
                     LLSELSLELQKLDAQIEEMETSKQVLQHQLESRMQSAGANVDAAGASVKEAELSKKQA
                     EDELRRTVSLAAQKLVSQRQLEQAQTAAKQAAENLNVVRAKATSSSAALSESRANAAQ
                     VDVLEKQIQRLRVNRESIQVQRERQASEVKKHTITSPVTGVVDQTFVNEGEYVAPGQR
                     FALVHDSRDVWINANIKETEVQKIRQGQPVIITVDAYPGRTFSGKVLLVGSAATSQFA
                     LIPSPNPSGNFTKITQRIPVKISVEQQDGLLKPGMMVEVDIDVSNR"
     gene            complement(13392..13838)
                     /locus_tag="PKI23_01700"
     CDS             complement(13392..13838)
                     /locus_tag="PKI23_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNN35683.1"
                     /translation="MPSSTDDIDRFVSLLTETSSLWRLHLSQRIKPTGISAAQWRVLS
                     NIKRAREPLTQSDLAGRVGIEAATLVSLLDRLEAAEWVERRPHPQDRRFKTVHLRDKA
                     LPILAELEIIAAQLRRELLGEIDPQELRIGTELLRKIQLKMEPKNR"
     gene            14056..15735
                     /locus_tag="PKI23_01705"
     CDS             14056..15735
                     /locus_tag="PKI23_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNN35684.1"
                     /translation="MSEQITHRPNHFIRQIVEADLARGANGGQLVTRFPPEPNGYLHI
                     GHAKSICLNFGLAEDYPGGRCHLRFDDTNPCKEELEYVEAIQRDVKWLGFDWHGPVRF
                     ASDYFEQLYGYAEELVGKGLAYVDSLSSEEMRSHRGTLTEPGRDSPDRSRSIEENLDL
                     LRRMRAGEFADGERVLRAKIDMASPNINLRDPVIYRVRHASHHQTGDAWCIYPMYDYT
                     HCISDALEGITHSLCTLEFEDHRPLYDWFLDHLSVPCHPQQIEFSRLDLQYTLTSKRK
                     LQQLVEEGKVSGWDDPRLPTIAGLRRRGYTPAAIRDFCRRIGVTKADNSVEMELLESC
                     IRDDLDHNAPRRMAVLDPLKLVIENYPDQIEWLQVPNHPQHEALGSRKLPFGREIYID
                     RADFRETAGKEFKRLVSGGEVRLRHGYVIRCDQVLRDDSGEIRELRCSHDPQTLGKAP
                     EGRKVKGVIHWVEASHAIAAELRLYQRLFVDANPAAADNFLDSLNPDSLTIVQGFVEP
                     ELATAQVGQGYQFEREGYFCLDPDSQPQRLIFNRTVALKDSFARIDQPAGR"
     gene            complement(15743..16042)
                     /locus_tag="PKI23_01710"
     CDS             complement(15743..16042)
                     /locus_tag="PKI23_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35685.1"
                     /translation="MIRIDAPGPIRTPVENNAPTENRRVNPVEPVRFYLTDEGERRID
                     RRRQPDRRISAQDRRMLNVGQRANNGRRIRKDRRRSALPRPTPQGRRGQLIDTQA"
     gene            complement(16080..16742)
                     /locus_tag="PKI23_01715"
     CDS             complement(16080..16742)
                     /locus_tag="PKI23_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNN35686.1"
                     /translation="MDFSEVIAQRYSVRAFRPDALPRALIERILDDALKSASWCNTQP
                     WQLIITEGAATDRFREALWAHVNSGKSANPDIPFPVKYHGAHYDRRKSCAMQLYASLG
                     IADGDRQASFAQTLKNFRLFEAPHVMIVTSHESLGSYGALDCGLFVQSFLLSATNQGV
                     GTIAQAAIAAYSDFIREHFAIAPEQRILCGVSFGYADPSHPANQFRTERATLEELVRW
                     EG"
     gene            16787..16993
                     /locus_tag="PKI23_01720"
     CDS             16787..16993
                     /locus_tag="PKI23_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35687.1"
                     /translation="MVALEQALIIRFRALGRIPAPLLLHSDNDLVFTSRRHTRLVRSR
                     GLQQEFITPTDHSRTAWWNGSSGH"
     gene            complement(17043..18773)
                     /locus_tag="PKI23_01725"
     CDS             complement(17043..18773)
                     /locus_tag="PKI23_01725"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNN35688.1"
                     /translation="MRASQILVSTLRETPADAEVISHQLMLRAGLVRKLASGLYSWLP
                     MGLRVLRKVERIVREEMDRSGAQELLMPVVQPAELWQESGRWEQYGAELLRIQDRHQR
                     DFCLGPTHEEVITDLIRNEIKSYRQLPANFYQIQTKFRDEVRPRFGIMRSREFIMKDA
                     YSFHADSASLQATYALMHQTYSRIFTRLGLQFRAVLADTGSIGGSASHEFHVLASSGE
                     DEIAFSSDSDYAANVELAEAVAVPPAQQLPAAALSRIATPGQSSIAELCQFLGVAPSR
                     TVKTLVVEGEATSDEQPAPLVALVLRGDHRLSEIKAAKLPGVAAPLTLASEARIRALL
                     GCGPGSIGPIGLPITTLVDRSAALLDSFVCGANEDGHHLSGANWQRDCPGITLVDLRR
                     VEEGDPSPDGRGRLLIKRGIEVGHIFQLGTKYSAAMNASVLDPNGRAIVMEMGCYGIG
                     VSRVVAAAIEQNHDARGILWPQAIAPFTVALIPLNYQKSEAVRQLTDRLYAQLAELGV
                     EVLLDDRNERPGVKLADCELIGLPHRIVIGDRGLAQGVVECQQRDAPSATELPPEQAV
                     NWLMQRLAAG"
     gene            complement(18858..19907)
                     /locus_tag="PKI23_01730"
     CDS             complement(18858..19907)
                     /locus_tag="PKI23_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNN35689.1"
                     /translation="MKIAIVSDAWEPQINGVVTTLRRTCAELTTLDHTAEVIGPDRFR
                     TLPCPGYRSIRLALWPGPRLARLLDELAPDAIHIATEGPLGLAARHHCLRHGFSFTTA
                     YHTQFPEYLALRTPIPLRWSYAAVRRFHAAAARTMVATASQQAVLEQHGFTHLVRWSR
                     GVDTALFRPGVKDFLQLPRPIAIYVGRVAVEKNLDAFLELDLPGSKVVVGDGPALADL
                     RERHPDAHFTGFKQGEELVRHLAAADVFVFPSRTDTFGLVLLEAMACGVPVAAYPVTG
                     PLDVVQPGVTGVLHEDLALAIPQALQLDPAACVAQAKACSWRNATLQFLNNLTPLRQP
                     AAATPPESAPDGHCH"
     gene            complement(19904..20704)
                     /locus_tag="PKI23_01735"
     CDS             complement(19904..20704)
                     /locus_tag="PKI23_01735"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNN35690.1"
                     /translation="MSPASLRARHYRTVWISDVHLGFSGCQADFLLDFLHHVTCDRLY
                     LVGDIIDFWQMRRGLYWPQSHNNVVRTLLGKAKHGTEVIYVPGNHDELLREHDGLQMG
                     NVAIQGRVVHQTADGRRLLVMHGDEFDSVVQCSRLLALAGSQAYALLLRANRLVNFVR
                     SRCGLPYWSLAGHLKHRVKNAVNFISNFESAVALAARRHDVDGVVCGHIHRAEISTIG
                     EVLYLNCGDWVESCTALVEHDDGKIELLHWSDQQHALKAETALPALAA"
     assembly_gap    21118..21217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21582..22667
                     /locus_tag="PKI23_01740"
     CDS             21582..22667
                     /locus_tag="PKI23_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HNN35691.1"
                     /translation="MKAAIMREVGKPLSIEEVQINKPGPREVLIRTAAAGVCHSDLHF
                     IDGHYPYPLPVIMGHESSGVVEAVGSDVTYVQPGDHVITCLSVFCGHCEDCLSGHMSR
                     CQNPEVRRSPMGPQPFSQDGKPIFQFLNLSSFAEQMVVHEHALVKIRKDMPLDRAALI
                     GCGVTIGVGAVIHTAKVEPGSTVAVIGCGGVGLSAINGAAIAGAGRIIAIDMQPAKLE
                     LAKRFGATDVVNPKDGDAVARVIEMTGGGVHYSFEAIGLKATAEQAFKMLRRGGAATI
                     IGMIPVGVMIELHGAELLTEKKIQGSIMGSNRFRIDMPRFIDFYMAGKLHLDEMVARR
                     VKLSEVNESLAALKTGETARNVIVFDH"
     gene            22753..23616
                     /gene="murI"
                     /locus_tag="PKI23_01745"
     CDS             22753..23616
                     /gene="murI"
                     /locus_tag="PKI23_01745"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNN35692.1"
                     /translation="MSIDPAHPSAPAPIALFDSGIGGLTVLQAVRRRLPAESLLYLAD
                     TLHMPYGEKSADWIVARSLALCELLLARGAKAIVVACNTATSAAAVTLRQRFAVPIIA
                     LEPAIKPAAALTRSGVIGVLATPATAEGTRLAGLIAQHAQGHRVLVQPCPGLVEAIES
                     NQSRALIRALLAERIAPLLDAGIDTLVLGCTHYPLVAEEIRALVGPDVVLLDTAEAVA
                     RELERRLSQERLLRVDARDSGELALHTSGDPLRLQQQLRHWMADLGDARVEESASLDL
                     PADRSPITRSL"
     gene            complement(23627..24820)
                     /locus_tag="PKI23_01750"
     CDS             complement(23627..24820)
                     /locus_tag="PKI23_01750"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNN35693.1"
                     /translation="MKIALQEAPAPQPPRLALFELGFRPFFALAGIAAVVQLLLWLTM
                     LQFHWPASAGYYPTLGWHVHEMLFGYAVAVVAGFLLTAVRNWTGRPTPVRWPLAALAL
                     LWLAGRLLPWCDTLLPHWLIALTDLAFLPLLATVIAIPLLQDRKPHNLVFPPLLLLMA
                     AANLLIHGERLADWPGSSLIGQRLMVDLLVLLLVIFGGRVIPLFSRNALPGMQPVHRE
                     WVERGALWSSWLFALLHPLPLPSALLATLAGSAALLHGMRLAGWHDRRIWRQPMLWVL
                     HLGYGAISIGYLLSAVSLLGGLSSSAASHTLTVGAIGLLTLGMMSRVALGHSGRLIAA
                     APLTRVAFMLLASAALLRVIVAACGLDSRWLLLSGLLWSSAFAAFVVVYLPILLRPRI
                     DGRPG"
     gene            complement(24859..26463)
                     /locus_tag="PKI23_01755"
     CDS             complement(24859..26463)
                     /locus_tag="PKI23_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNN35694.1"
                     /translation="MKSAEGRHPVPTGLMAGPRILALFAEYGPSYRWYAVFTVMLASF
                     TTLLTGTIINVAIPDIMGTFGMSSDQAQWVSTGYLAATTGFMLLTAWSVEAYGMRFTY
                     LAAMGIFLVGSLMGGFAPTAEVMIISRIVQGIAGGLMGPLSMLLITQVFPLDKRGMAM
                     GIFSVGVVLAPAMGPTIGGLLVDTFSWHYVFHIQVPLSLLSFPLGILFMPAREGSGPR
                     TPFDWNGLVLLAIFVFTLLTALANGQRYGWNSDYILTLLAVSLVSGVAFIAWEHHSPH
                     PMLNLEIFREPRFAAAAGISFILGAGLFGSTYLVPLFLQTVAGVTPTTSGLLLMPAGL
                     AMAALNPVSGFLSDKIDARLMIWFGLFTFSVSSLLLANIDINTSFFTLIWWVLIGRVG
                     LAAIFPSLNSGALRVLPFRLMSQGSGATNFIRQLGGAFGVNLLTVALQDRISFYYDAF
                     ATTQTSGNVVTLELLNKVRALLAANGLPEAQQLPAAIGYLGQMIGSQAATFAYRDTFL
                     GITLIFLLTMLPTALLKLDMPGRVAR"
     gene            complement(26450..27556)
                     /locus_tag="PKI23_01760"
     CDS             complement(26450..27556)
                     /locus_tag="PKI23_01760"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNN35695.1"
                     /translation="MNDASSATLTPTQGRRTLLLGLFAVACLIGAGYWLYQRYTHVYI
                     SDARIGASMVSVSSRAPGWVTDLMVSASDRVKKDQPLLTLDARDTTLRLREIEAQIAI
                     AETDLARQKLEHDLLDRQTTNRLDAARSRQEAVQTTLATAQSNYNFAKSELERARTLR
                     QQRLLAEREWDSKQTALDTARQAVERSRAELAAAHSTLLEAEASRDQLRQLEQTILRT
                     QHQLEQLQANRQRQQIDLDDRTVLSPIDGVIDETFTHDGEFIGTGQRILLMHAPDQIW
                     VDANIKETDLDHLRLGQQARITVDGYPELAPEGKVVRIGNAAISQFALLPNANPSGNF
                     TKITQRIPIRIAVDQVEGDLLRPGMMVEVAIEVR"
     gene            complement(27549..28022)
                     /locus_tag="PKI23_01765"
     CDS             complement(27549..28022)
                     /locus_tag="PKI23_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNN35696.1"
                     /translation="MQESSSARELFGFKLVHLARCWRMRLDQQLKHTGLTQSKWNALV
                     YLTRGGDGLLQKDLARYIGIEGPTLVRLLDDLQRQGLIERRSTHRDRRGKTIHLTDAA
                     YPVLTEFHQAAEALRAQLLDGVSEQEMALCIDLFERLLHNLNTIKPAREQSNHHE"
     gene            complement(28315..29943)
                     /locus_tag="PKI23_01770"
     CDS             complement(28315..29943)
                     /locus_tag="PKI23_01770"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNN35697.1"
                     /translation="MATAVKLEPAEQYDAIIIGAGIAGMYQLIKLRELGCSVRVFETG
                     DGVGGTWYWNRYPGARFDSESYSYGYSFSEELLQEWEWSEHFSPQSETLRYLNHVADK
                     FDLRRDIQFNTRVTAAHYSEADSCWTVTTDTGTQARARFLITAVGPLSAATLPAIEGI
                     DSFKGESCHTANWPHHEVDFTGKRVAVIGTGATGVQVIQEVAKSAKQLTVFQRRPNWC
                     TPLNNRPITPEEQRQIKASYPQIFQQCRETNGCFLHKSDPRSALEVSPEEREAFFEHL
                     YQQPGFAIWIGNFMDILMTPEANRTISDFIAKKIRQRVKDPKTAEKLIPTDHGFGTRR
                     VPQETHYYEVYNQPNVELVSLIETPIERITPQGIKTSDREREFDIIIYATGFDAITGA
                     LDRIDIRGVNGLKLKDKWQDGPRTYLGLQSVGFPNLFTLVGPHNTATLCNIPRCIEQN
                     VEWVSGVIGHLRSHQLQSVVPSEEAERSWTQHVNEIAEYTLFTKVNSWFTGVNTNVPG
                     KDKPRIVVYVGGAPAYRERCDQESANGYPGFVLQ"
     gene            complement(30095..30631)
                     /locus_tag="PKI23_01775"
     CDS             complement(30095..30631)
                     /locus_tag="PKI23_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HNN35698.1"
                     /translation="MVSPLQEQLLKAGLVNSNQVKKAAKEKYQASQQPGNADPTAEQI
                     RQAQQQKAESDRQRNLEQQQAREQKALAAQIRQLIETQRIDRSGGTVSHQFVDHGKIK
                     KIVLHAQQREQLIRGTLAVVRLDERHELVPVGTAQKIAQRDPGVVVVLNAPPSSAAED
                     ANETDPYADHPIPDDLMW"
     gene            complement(30644..31771)
                     /gene="hisC"
                     /locus_tag="PKI23_01780"
     CDS             complement(30644..31771)
                     /gene="hisC"
                     /locus_tag="PKI23_01780"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNN35699.1"
                     /translation="MTEPSPPPEELHTRLEQWIRPELRSLAPYHVADASGCIKLDAME
                     NPYPWPGTLTTAWLERLSSVELNRYPDPQGSAVKQALRRHFAIAEHHALLLGNGSDEL
                     ILLLALLFGGTGHCLLAPEPGFVMYSLIARQAGMAYCGVALAEQFELDRTAMLAAIAE
                     RQPAVIFIAQPNNPTGNLFDPATLRAVIEAAPGVVVIDEAYCAFTDTQHLPLLDEFPH
                     LLVMRTLSKIGLAGLRLGLLIGAPALIERLDSLRLPYNINSLTQASACFALEHASLLT
                     EQSQQLRRERSRLFAALQSLPGITPYASEANFILLRVPAGEAPRLQRLLKARHRILIK
                     CLDGGHPLLRDCLRITVGTPAENDALLDALTQLLCEPPSNT"
     gene            complement(31768..33096)
                     /gene="hisD"
                     /locus_tag="PKI23_01785"
     CDS             complement(31768..33096)
                     /gene="hisD"
                     /locus_tag="PKI23_01785"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNN35700.1"
                     /translation="MTNPTLITRIDSRATDFMQQLDSRLGFEMEEDLLHTNQVRSLIE
                     QVRREGDAALLALTQRFDRHPAQTMAALELDCGRLDAQLQNLPAAGVAALQHAAQRIR
                     AFHALQRQPSWRIEDEAGFTLGQEVRPLDRVGVYVPGGRAAYPSSVLMNVIPAKVAGV
                     GEIIMVSPTPDGTLNPWVLAAAAIAGVDRLFTIGGAQAVAALAYGTASVPRVDKIVGP
                     GNRYVATAKRLLYGQVGIDMIAGPSEVLILCDGKTPAEWVVMDLFAQAEHDEEAQSIL
                     ICPDAAYLDRVTDTMARLLPAQPRAAIIAAALQKRGLLLQVTDLQQGVELINHIAPEH
                     LELSVADPEALLPSIRHAGAIFLGRHACESLGDYCAGPNHVLPTSGTARFSSPLGVYD
                     FQKRSSIIGCSSQAAARLADTAITLARLEGLEAHAAAAECRLREPGSTTP"
     gene            complement(33093..33749)
                     /gene="hisG"
                     /locus_tag="PKI23_01790"
     CDS             complement(33093..33749)
                     /gene="hisG"
                     /locus_tag="PKI23_01790"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNN35701.1"
                     /translation="MSPLTIALTKGRILDECLPLLRAAGIEPLDSIAESRKLIFATNQ
                     PDVRLLVMRGTDVPTYVRHGAADLGVAGKDMLLEQGSSGLYEPLDLGIARCRLMTAGP
                     VVAPPRRGRLRVATKFVNVAKRFYGERGEQIELIKLYGGMELAPLVGLADQIVDIVDT
                     GKTLQANGLAPLELIADISSRLIVNKASMKVKHRAVSALIEQLSAAVADRNRLQAGQP
                     "
     gene            complement(33778..35040)
                     /gene="murA"
                     /locus_tag="PKI23_01795"
     CDS             complement(33778..35040)
                     /gene="murA"
                     /locus_tag="PKI23_01795"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNN35702.1"
                     /translation="MDKLIITGGVRLDGEIRISGAKNSALPILAATLLCDEKMEIGNL
                     PHLHDITTMIELLGCMGVEVMLDEQMRVEINTRTLHTPSAPYELVKTMRASILVLGPL
                     LARHGQAEVSLPGGCAIGSRPVDMHIRGLETMGAQIDLEGGYIKARVDGRLKGAHIFM
                     DMVTVTGTENVLMAAVLAEGRTVIENAAREPEVVDLANCLNAMGAKVSGQGSDTLVIE
                     GVERLHGCRFDVMPDRIETGTYLVAAAATGGRIRLKDTAPVILEAVLHKLEEAGAAIE
                     TGPNWIQLDMKGERPRAVNLRTAPYPAFPTDMQAQFTVLNSIARGTGTIIETVFENRF
                     MHVHELNRLGANIALEGNTAIVSGVDHLRAAPVMATDLRASASLVLAGLVASGQTEVE
                     RIYHIDRGYECIEEKLLQLGAQIRRVAA"
     gene            complement(35089..35325)
                     /locus_tag="PKI23_01800"
     CDS             complement(35089..35325)
                     /locus_tag="PKI23_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNN35703.1"
                     /translation="MQLDEIKALIETGLPGSQAQVDGDGYHFQVVVTWDRFSGMSPVK
                     RQQQVYGCLQANIASGALHAISMKTYTPDEQQRA"
     gene            35555..35998
                     /locus_tag="PKI23_01805"
     CDS             35555..35998
                     /locus_tag="PKI23_01805"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNN35704.1"
                     /translation="MTTTWILVAERSRARIFTLQNRVAPLQELESLVHPQSRLKESEL
                     VSDHAGKLSDKGQPGTHAAPSATSAKEKEAELFARQLCQRLEQGRAQQQLERLVVIAA
                     PDFLGLLRQQMGAALRALVVAELDKNLAMASAEQIRSHLPERLAQ"
     gene            36286..38547
                     /gene="parC"
                     /locus_tag="PKI23_01810"
     CDS             36286..38547
                     /gene="parC"
                     /locus_tag="PKI23_01810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNN35705.1"
                     /translation="MDFTTTFSEDGSEQVDLRLYTERAYLDYSMYVILDRALPHLCDG
                     MKPVQRRIIYAMSELGLASGAKFKKSARTIGDVIGKFHPHGDSACYEAMVLMAQPFAY
                     RYPLVDGQGNWGSTDDPKSFAAMRYTEARLTAYAELLLGELEQGTVDWLPNFDGTLEE
                     PARLPARLPNLLLNGASGIAVGMTTDIPPHNLREVAAACVRLLDEPDTAIETLCELIP
                     GPDFPTEAEIITPPDELRKIYSEGRGSVRLRSRYEQEEGEVIIKALPHQVSGSRVMEQ
                     IAEQMQARKLPMVVDLRDESDHENPTRLVLVLRSGRVEVDALMNHLFATTDLERSVRV
                     NFNLIGLDGRPQVMDIRQLLGDWLQFRRDTVRRRLNHRLERVLERLHVLEGLLIAFLN
                     IDEVIRIIRNEDEPKPALIARFALSERQAEAILELRLRHLARLEEMKIRGEQAALAEE
                     RDHLQALLGSETLLTRLIRDEIERDAERYGDARRSPLVARGAARQLDEEALLPNEPIT
                     VILSSNGWIRAAKGHEIDPAALSFRTGDGLLQAVRGRSQQESILLDSTGRVYAISSHG
                     LPSARGQGEPLTGRLAPPANSRFVALLMGEAGQTWLIASDAGYGFIVRHEDLLSRNRA
                     GKALLTLPAGAQVLPPRRVADLESDRLALVTNDGRLLICALAELPRLARGKGNRLIAI
                     PAQRAQEREEYLLAAEVLGAQDGLVIHAGKRHLTLNPADLIHYQGERGRRGLKLPRGL
                     QRVERIERLARGG"
     gene            38600..>39655
                     /gene="serB"
                     /locus_tag="PKI23_01815"
     CDS             38600..>39655
                     /gene="serB"
                     /locus_tag="PKI23_01815"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNN35706.1"
                     /translation="MAKILLITLSGSDQPGITAAITAVLAPYDVQLLDIGQAVIHDTL
                     SLGLLIELPTAAESSPVLKDVLFCAHELGVQVRFTPVSLDNYRRWVEHQARSRYIVTL
                     LARKISALHLARVTRIVRDHELNIVGINRLSGRIALDADPARTQACVELSLRGEAVDL
                     PGLRAQFLAIANELDVDIAFQEDNVFRRNRRLVAFDMDSTLIEAEVIDELAKRAGVGA
                     EVAAITERAMRGELDFTDSFTRRVALLKGLPESVLAEVADQLPLTEGAQRLIDTLRRL
                     GYKTAILSGGFTYFGRWLQQRLGIDHVHANELEIENGRVTGRVLPPVVDGLRKAELLR
                     QIAAQERISLDQVIAVGD"
BASE COUNT         6855 a        12048 c        13419 g         7233 t
ORIGIN      
        1 gtcgtgttac cggcttgtgc cacaccaagg tgtggggctt gctcctgtgg ttctgcttga
       61 tgcggtctgt ttgccgggga gagccttttt gtttcgacct gttggttgtg cagttgcttc
      121 ttcagaacga gattgttggt cagctcctct tccagcgagc gggcgtagcg atcgatctct
      181 tcgctcaact cgtcaagcgc ctgctgatgc gccttgcgca gagcgtggat ctcctgctga
      241 tgacgctgtt cgatggcctg cgtgtcgaat ccggccgtgc cgatggagtt cgcgtgttgt
      301 gcgaaggtgg cggaagaaag cggtctggag ggttcgtccg caaaggcgag gcccagccgg
      361 ttccggctga ttgcctcgtg aatctgcacc agcacattgt gttcacgctg gaggtcgggt
      421 tcccacaggt gggtctggag ccatggcagc ggacagtggt tgaggctgct gagacgcagg
      481 ttggtctgat cagtgatggc gaccggttcc gcatagtgaa gaagctgctc aagcggcaga
      541 tcccattggc gatcggcata gccatcgtca tcgaagccga tcctgaacag caccacggtt
      601 tcgatcacca gacccggcag aatggtgacg taggcggcgt cgatcatctg gccggcaaaa
      661 tcggcaacgc ccaccacctg gtcgagcagt gcgtcgaatt cgactttcag catcgttctg
      721 cacagtgcgc cgtgctcgaa aaagagcacc gcctcagcgc gcagtgccgt tgcggggttc
      781 atgcaggaat ttgccttggt ttgatcatgt catctgcaat agaggaggcg ccctgtcgaa
      841 gtatagtggg gcattggctt ggcacgttgt gacgagcttg gcacaatcag cactgtcggt
      901 ggtggctgcc cggtggtggt gccatgaccg gacgcaccag caggaggcag tcgccgtgtt
      961 tcagacagaa gagtgtcaag attttttaca gttttgattt tggcgcggag ctggattttt
     1021 tcaatagctt gatccagaac gaataaagca tattggccta atgtttgcat ttttcaattt
     1081 gctgtcgtaa ctgactgctg acctcaaccc ggaacccaag gagtgatgta tgaaaatgat
     1141 acccaaacgt ttcgcaatgg ccctgctgat ggtggccagc gcggtgttct ctctcaacag
     1201 ccatgccgcc accatcgacc tggggaaact cggggtgggt gatactgagc ttgatatcag
     1261 tatcgcgttt ggcagcattg ccgatacttg gaaattcgaa gtgaccgaga caggcaagct
     1321 ggttagcggc tttgccggaa tcttcggcat cgcttcgctg agcgccgata tctcgggaac
     1381 tccttttgcc tcagttcctt ccccgttgcc attgctgtcg cctgttctgc tggggccctc
     1441 catcgtgttg agccccggca tgtacagcat ttcggtgacc ggaaccaccg gtactggctt
     1501 tttccccatc ggcagctaca taggtgcggt tgggctgact gccgtgccgc tccccgcacc
     1561 ggttctgttg ctgggttcgg cgatgctggg tctggtcggt ttcgcgcgtc gccgccgcgc
     1621 atgaggcttc gcagccaggc tgtctggtct tgagagggat cagacagcgt tggagaaaaa
     1681 acgccctgaa tttcagggcg tttttttcga gaatcaaatc gtgcctgctt cacttcactg
     1741 cctgtgcaag aagattggga cgctttggca gcgcaatgac ggtggcttga ccgtcacgct
     1801 ccgcgacggt tcgaggagag ccgtggaagg catcgacagc cgagtccgtt ggcggcaaac
     1861 tggttcgcat gcctttggga gcagagcgaa caggctgtcg cgggagtgag ccatggtccg
     1921 ttttcgtgat cgtcgacatg cgggcgaagt gctggcgcag cggcttgcgc cgtggattgg
     1981 ccgtactggt gtggtgctgg cgctgccgcg tggaggagtg ccgctggccg cggtggtggc
     2041 gccaagactc cggatgccgc tcgacctgat cattccgcgc aagatcggcc atccccacaa
     2101 tccggaatat gccgtggcgg cagtggtcga aagcggcgcg cgggccatca acagcgtgga
     2161 ggtggccggc cttgatcctg gctggttcga gcaggccgtc gcgcgtgcgc agcaggaggc
     2221 cctgcgtcgt cggcgctgct atctgggtga gcgttcgccc tgtgcgctcg agggcaaggc
     2281 ggtgattctg gtcgatgatg gcattgccac tgggctcacg atggaggcgg ccattcagga
     2341 tgccaaggcg cggggtgcgc aggagttggt ggtcgccgtg ccggtggtgc cggccgacac
     2401 ggccagacgt ctggccagga tggtgtcgca ggtggtggcg gtggagattg cggagcacta
     2461 ccttggtgcg gtaggcgcct attacgatga tttctcccag ttgagtgacc aggatgtgat
     2521 tgaactgctc aagcaggccg ctcctgcggc gccgtcctga cacgccgggg ctgttgagtc
     2581 ggaattcatg gcatccgtga gtgagagtga gtggcgcgga gcggtgaccg catgacgtcc
     2641 accctcactt tggcggaaaa ggtcgcgttg ctgcgcgatc cgcacaccca tgcgctgccg
     2701 gcgcagagcg tcatcgaagt ggtggagacc cgcatgtcct ggctgtttct gacaccggaa
     2761 cgggtctaca agctcaaaaa gccgttgcgc gagccgctgt tcgatttttc caccttggcg
     2821 cggcggcggc tcaattgcga gacggagctg cggctcaacc ggcggttggc ggccgaggtc
     2881 tacctcgatg tggtcaggct gacactgagc agcgcggggc tgtgccggat cgatggggag
     2941 gggcagacgc tggattacct ggtgatgatg cgccggctgc ccagggcgcg cagtctcgac
     3001 cagttgattc tcgctggggc cattccgcct gacgcggtcg aaatgctggt gcgacgattg
     3061 ggggacttct atcgacaggc ggaaaaatca ccgcagggtg cgaccggcta tgtggcgcac
     3121 tttcgcgagc ggatcgagca caaccagcag gcgctgattg ccggagacta ccctctgtca
     3181 cggcaatgga tcgagcagtt gcatggccgt ctgctggcct acatcgacca gcacggtgaa
     3241 acgctgcagg cccgcagtcg ccagcggcgg atcgtcgagg ggcatggtga tctgcgtccc
     3301 gagcatgtct acctgatcga tggccagccg ttggtcatcg actgcctcga attcagccgt
     3361 gaactgcgtt ggctcgatcc gatcgacgag ctgagcctgc tcgacctcga atgtcttcgg
     3421 ctgggcgagc ccgattttgg ccggacgctg ctggccgaat atcaggccat cgagcaggat
     3481 ccggtgcctg accatctggt cgatttttac cacggctatc gtgcgctgct ctggagccgt
     3541 ctcgccatcc gtcatctgga tcagccgcag cgcaaacccg gagatggtgc aaagtggcgg
     3601 gagcgtgccc ggttttatct gcagcaagcc gcgcgggtcc tgcggcagcc ggaagctttt
     3661 ggttgatcga ccggaggccg ggttggcagc cgtctgcatg aaccggtgtc atgtctcggc
     3721 atcgagccac agctcggtca gcgactcgcg ccggtgaatc cgcccgatcg cctcggccag
     3781 cagcggcgcc accgaaagca gctcgacgcg ctgctgcagc actggattgg cgcgctggag
     3841 cagtggcagg gtgtcggtga ccaccagggt ggtgagggcg ggatgatcga gcagtgcatg
     3901 gctcttgccg gcccacagcg catgggtcgc ggcagcatgg atccggcgcg cgccagcccg
     3961 ctctgcggcc tcgatggtgc gcagcagggt ggcccctgat gcgatcagat catcgatgat
     4021 caccacctcc tgcccggcga catcgccgac cagccgttcg ccgctcagtt cgccacggct
     4081 gcgccacttc tgcatgaagg cgctgttcag ctcacgtccg aggcggcgac tcaaagagcg
     4141 acgcagcgcc tcggcccgtt tcacaccgcc gacatcgggc gagaggatgc aggcatttcc
     4201 ctccagcagg gtggcgaagt ggtcgacgaa caggctcttg gcttcgaggt gttcggtcgg
     4261 aatgcgaaag gcattctggt aggccgccag attgtggacg tcgatggtca gcagtcgatc
     4321 acttcccacc gcctcgaaca gttgggccac atagcgatgg gtgaccggat cgcgcggctt
     4381 gctcctgcgg tccttgcgtg cataggcgag gtagggtgcg acgacggtca cttcggtggc
     4441 gccggcatcc ttgagtgcag cgacaaagaa cagctctctg accaggcggt cattcaccga
     4501 atgagccgca tcgctgtaga gcgattgcag cagcacgaca tggtggccac ggacgctttc
     4561 gaggggtctg gccttgtgct cgccatcctc gaattggcgc tcttcccagg ccgaaagcgg
     4621 agctccgagc gccgaagccg ttgcgaaagc aagcgaggag ttggagtcga gatcgaatat
     4681 ggacaatggg ttagtcacgt attttcatcc aaaatctgat atcagagggg tgtgactgta
     4741 gcctccatca aagtttcgcc gcgatcaact attgattaga ctgctggcga ttgccaggtt
     4801 gtggaatatt tcgcccgtac agagtagtgc cgcccatgaa gaccatgcac aaatatgaag
     4861 tgccaatgac cgatcaaccc ttccacgtcg atctgccgca tgacgcccag gtgcgctggg
     4921 tcgaatttct ggtccccaac aagaaggtgt tcatgtgggt cgaggtcgac atcagtcgcc
     4981 aggggcccaa gcagcgtcgc accttccacc tctaccgttc cggcgatggc attccggtca
     5041 gctaccacta tgttggcacg gcggtggatc agtacattcc cgagacctac cacctttttg
     5101 aaaaggttga ttgatccgtt gccgccgcga gcgtgtggtt gaagcatgaa caggttctgg
     5161 tcggagtcag tcaaggcgcc gttgccgggc cattcgacgc ggcgccggtt ggtgctgctg
     5221 gcactgctgg gtctgcttct ggcagtggct gcaccccgac agcacgccga atgggtcaat
     5281 tcactggcgc acgaacagag tgggcagagc agtgccaacg accaggagat ggcggcgaat
     5341 caccactgcg gtcatcacgg tacccctgcg ggaggcagtg gcctcgggtg tggttgcctg
     5401 cacggtctgt gcggcggtct gactgcgctg ccgggtcatc aggcgctctc cgcgcgggtg
     5461 cgcattgcaa acttcagccc tgcactccag cggaccgtgc cattgccggc ccatgggcgg
     5521 ccacctttca ggcccccgat cagctgaact cctgagcttg ccactgccgt catccgcatc
     5581 tgtgcggagg cggctgtcga ccatgcgatt tggagtcaaa gatgtcatcc acaaagagca
     5641 cccctgccgg gttcgattgg cggcgccgtc gcctggtgca ggggattggt gtcgggttgg
     5701 cggcgggagt ctgtggtctg gcgccgcggt tcggccaggc cgaccctgga gcggagttca
     5761 cggcttcggc agtggccagc gccaccccga gggagctgcg cggcacccaa ttcgatctca
     5821 ccattgccga gacaccggtc aacttcaccg gccatccgcg gctggccacc accatcaatg
     5881 gcgcgctgcc ggcgccgacg ctgcgctggc gtgagggcga tacggtcacc cttcgcgtct
     5941 acaaccgtct ggccgaagag agctcgatcc actggcatgg catcctgctg ccctatcaga
     6001 tggatggcgt gccgggcatc agttttccgg gcatcccgcc tggcggcacc ttcacctatc
     6061 gcttcaaggt ggcacagagc ggcacctact ggtaccactc ccactccggc atgcaggagc
     6121 agcgcggcct gtatggcgcc atcatcatcg atccgatcga gggtgagcgg attcaggccg
     6181 accgtgacct cgtgctgctg ctgtccgact ggagcgacga ggagccgatg cagctgctga
     6241 agcggctgaa ggtgcagagc gactactaca acttcaacca gccgacgctg gccgacttcg
     6301 tgcgcgatct ctccagcgcc ggctggtcgg ccgccatcga acgccgccag atgtggcagc
     6361 agatgcggat gaacccgacc gatctggccg acatttcagc ccacacctac cgctacctga
     6421 tcaacggcat gacgccagcg gacaactgga ccgggctgtt ccgccccggc gagcggctgc
     6481 ggctgcggct gatcaacggc agtgccatga ccttttacga tctgcgcatc cccggactgc
     6541 cgctgacggt ggtgcaggtc gatggccaga atgtcgaacc ggtcaccgtc gatgagctgc
     6601 ggttcgggcc cggtgaaacc tatgacgtca tcgtcgcccc acgcgatgat gtctatacgc
     6661 tcttcgccca gtcgatggac cgctcgggct ttgcccgtgc cacgctggcg acgcggccgg
     6721 gcctgaccgc cccggtgccg gcgctcgatc cggtcgaatg gctgtcgatg acggacatga
     6781 tgggcgacat gcatggcggc aggccggcgc accatgcccg cagcgaacat ggcccgggga
     6841 ccgacatgcg ggtcgacagg gcacgcagca atctggacga tcccggcgtg gggctgcgca
     6901 acaatggtcg ccgcgtcctg acgctggccg atctgcacag cctggagggc gtgctcgatc
     6961 ggcgaatggc cgaccgcgag atcgagctgc acctgaccgg caacatggag cgctacagct
     7021 ggtcgttcga tggcgtcgag tacggccaat cgacgccggt gcatttccgc cacggcgagc
     7081 ggctgcgggt gatcctgcac aacgacacca tgatgaccca tccgatgcat ctgcatggca
     7141 tgtggagcga actggagagc ccagccggag ccgttcagct tcgccgccac acctttgccg
     7201 tgcagccggc gcagcggatc agctttctgg tgacggccga tgcactggga cgctgggcct
     7261 ggcactgcca cctgatgttc cacatggaca gcggcatgtt ccgcgaggtg gtggtggcat
     7321 gaaccctctg agcaatgaaa agaaaagtgc gtttcatccc gcagggtggt tgaaaccgct
     7381 gctgctgacg gccgtactgg cactgaccag gcaggctgcg gccgagaccg ctggtcatgc
     7441 gcaccctcat gcgatcgaag agccgccggc aaagacgatc gaccacagcc ggatggacca
     7501 cggcagcatg gaccacggca gcatggacca cggcagtggc gattccgccc gcgaggggcg
     7561 tgatcccgat gcctactcga atggctaccg gctcgattcc ggcccctatc tgctggaggg
     7621 ccggcagcac tcgcacatgg ccgactcgca ccgcttctcg gtgctgcgca tcgatcggct
     7681 ggagcgggcc gatgggcgcg atcacaccgg cacggcctac gacgcgcagg cctggtgggg
     7741 cggcagctat gatcggctct ggttgaaggc cgaaggtgag cacagcagcg gcacgctgca
     7801 ggaggcgcgc agcgagctgc tctggagcca tgcggtcgcc ccgttctggg accggcagct
     7861 gggactgcgc catgacagtg gtgcagggcc ggagcgccag tggctggcgc tgggcctgca
     7921 tgggctggcg ccctactggt tcgagctcga tgtggcggcc tatctcggca gcggtggccg
     7981 cagtgcgctg cgtcttggca gcgaatatga gttgctgttg acgcagcggg cgatcctgca
     8041 gccgcgggtc gagttcaacc tctatggcca ggatgacccc gcgcgcggtc tgggcagcgg
     8101 ccttgccgaa ggggtgctgg gtctgcggct gcgttatgag ttcagccggc agcttgcgcc
     8161 ctatctgggt gtcgagtgga gcggaaagtt tgggcagagc gccgatctgg cgcacagtgc
     8221 cggtgatgcc accagcgaat ggcgctgggt tggcggagtg cggttctggt tctgaagacg
     8281 ttggcatcga ccacaggggg gctcgagatg acacagcatg acgatccggc ggcttcggcc
     8341 gctcgccacg cgggaggctg ctgcggtcag gacacagagg cagcggcggc ggatcgggac
     8401 ccggtgtgcg gcatgagcgt caagcccgac tcgccacacc gcagccgtca tgccggtcag
     8461 gagtaccgct tctgcggtgc gcgctgcaag gagcggttcg acgccgatcc gcagcgctac
     8521 ctgcatcccg tcgcgctgcc cgccgaggcg atgccgctcg atgtcgagta cggctgtccg
     8581 atgcatccgg aggtgtcgca gatcggccct ggcagttgtc cgaagtgcgg catggccctc
     8641 gaaccgctgc tgccgacgct cgatgagggt gaaaatccgg agctgaccga ttttcgccgc
     8701 cgtttctggt ggaccttgcc atcgagtctg ttggtggccg tcatctcgat gtcgggagag
     8761 ctgttgcagc cgctgctggg ctcgatgcag ggctgggtcg agctgctgct ggcgacgccg
     8821 gtggtggcat gggccggcgg cccctttttc gtgcgtggca tccagtcggt tcgacagcgc
     8881 agcctcaaca tgtggacgct gattagcctc ggtaccggtg ccgcctacct ttacagcctc
     8941 acggcactgc tgctgccggg cctgttcccg gcctcgttcc agatgcatgg ccgggtcgcg
     9001 ctctatttcg aagcggcagc ggtgatcatc tcgctgacat tgttcggtca actgctggaa
     9061 ctgaaggccc gtgcgcagac cggtgcggca atcaaggcgc tgctggggct ggcgccgaag
     9121 agcgcgcgtc gcctgcgcag tgatggcggt gaggaggagg tggcgctgac ccgggtgcag
     9181 cgcggcgatc tgctgcgggt tcggccgggt gagaaggtgc cggtggatgg cctggtggtc
     9241 gaaggggaga gcgccgtcga tgagtcgatg ctgaccggtg aaccgatccc ggtcagcaag
     9301 cgggtgggcg accgactgat cggtgccacc ctcaatggtg gcggggcact ggtgatgcgc
     9361 gccgagaagg tcgggatcga gacggtgctg tcacagatcg tgcagatggt ggcacaggcg
     9421 caacgctccc gtgctccgat gcagcggctg gccgaccagg tggcgggcta ctttgtgctg
     9481 ggagtgatgg cggtcgcgct gctgacgctg ctgggctggg gtctgttcgg accacagccg
     9541 agctggatcc atggcttgat caatgcagtg gcggtgctga tcatcgcctg cccctgcgcg
     9601 ctcgggctgg cgacgccgat gtcggtgatg gtcgccaccg gccggggcgc cacccagggg
     9661 gtgctgttcc gtgatgcggc cgcaatcgaa ctgatgggca aggtcgacac gttgatcgtc
     9721 gacaagaccg gtacactgac cgagggcagg ccggtcttcc accaggctgt ggcctgtgcc
     9781 ggatggagcg agtctgaact gctgcgcatc gctgccagcc tcgatcaggg cagcgagcat
     9841 ccactggccg ccagcatcgt ggcggaggcg cgacggcgtg acctgccgct cagcaagccg
     9901 gagcagttcg aatcttcatc cggcatcggt gtgcgcggcc aggtcgatgg gcagccggtc
     9961 gtgctgggca acggtgcact gatgagcgag gccgccatcg actggcagcc gctggcccag
    10021 caggccgagg cactgcgcgt cgagggggcg acggtgatct ttgtcggtgt ggcaggccgg
    10081 ttggccgggc tgattgcggt gatcgatccg atcaagggca ccaccgccga agccctgcgg
    10141 gcactgcgcg ctggcggctt gcgcatcatc atggccagcg gcgatggcct caccacggcg
    10201 cgggcggtgg ggcgggcact ggggatcgac gaggtgcatg gcgaggtcaa gccacagcag
    10261 aaggcagagc tggtggccag attgcaggcc gaggggcaga tcgtcgccat ggccggcgat
    10321 ggcatcaacg atgcgccggc gctggcacag gccaatgtcg gcatcgcgat gggcagcggc
    10381 accgacgtgg cgatgagcag tgcccactgc acgctggtca agggcgatct gcgcggcatc
    10441 gagaccgcgc ggcggctgtc gctggccacc ctcggcaaca tgcggcagaa cctgctgttc
    10501 gcctttgtct acaacgcgct gggggtgccg attgccgccg gattgctcta ccccttcacc
    10561 ggccttttgc tgtcgccgat gattgcggcg gcggcgatga gcttcagctc ggtgtcggtg
    10621 gtgagcaatg cgttgcggtt gaagcgcgcg cgaatctagt gcgtcgagcc ggcgggcagc
    10681 tccgcgtcgg cctcctcgcc atccatcggt atccgctggc cgcgcggctt gcagaagcgg
    10741 cccagcacca gtgccgggat gatgccgagc agaaagacga tgccgacgac gatgaaggca
    10801 tcgcgaaagg cgaacatcga cgccttggcc gcgaccacct gtgccagata ctgcaccgca
    10861 ccggcgttct ggtgcgcctc cgagacgccc gactgcgcat agagttgcgc catcgagcgg
    10921 atcagtgcct gggtggtgct gttgtcgggt atctgggtgg cggccagaaa gtcggcatag
    10981 aggtcggtgc gtgcgctcat gtagaccgac agcaggttga caccgaaggc gccgcccagt
    11041 tggcgggtga agttgatcgc gccgctgccc tgtcccatca gatggggcgg caatgacccc
    11101 atgccaccga cgctgaccgc cggcatgatg aaggtgaggc cgatgcggcc gagcatgatc
    11161 atcgacgcca ttgtccagaa ggtggtgtcg acatcggcgt cgtagagcag caggctcgac
    11221 agcgcgaaga tcacgaagcc ggcgagaatc atgtaatgcg gacgcacatg gtcggagagc
    11281 cgtcccatcg gcagaaacag catgcccatc accagaccgg ccggcatcag catcaagcct
    11341 gagagggtcg gggtgtagcc gagctgcaac tgcacgaaca gcggaaagat gtaggtcgag
    11401 ccgaacaggc cgatgccata gatgatcgag acgccggcgg cggaggcaaa gcccggatag
    11461 ctgtagacgc gcagttgcag cagcgggcgg tcggagttga gttcccacca cagaaaggcc
    11521 gcggcgctga cgaagccgac gctgaacagc gtgacgatgt agtcgctgta ccacccttcg
    11581 cgctggccat tcgacagtgc ggtcagcacc gtcagcagaa aggtgcagag cagagcaagt
    11641 ccggtccagt cgaagcggcg agctgggccc tcatcctccc gatcggggag aaaaaccgca
    11701 gccatggcaa tcgccagaat gcccacggtt ggcgcgacga agaacactgc ccgccagttg
    11761 aagttgtcgg ccagcacccc gcccagtgtc ggaccgaagg ccggcgccag aatgacaccg
    11821 atgccgaagt agcccatggc gttgccgcgc tgatcctcgg gaaagacccg gaacatcatc
    11881 tgcatcgtca gcggttgcag cagacccgcg gcggccccct gaatgacgcg tgccagaatc
    11941 agcgtgttga tgtcctgccc caggccaccg agtatcgagc cgaagatgaa aaagagcatg
    12001 gccgcgatga aggtcttgcg ctggccatag gcatgcacgc accaggcgtt gaccagcatc
    12061 gaggcggtgt tggcggccag aaaggcggtc gacatccact gccctttgtc ctggccgacg
    12121 ccgaagatgc ccatgatgtc gggaatcacc acgttgacga tggtggtgga gagcgcggtg
    12181 gtcagcgtgc cactgatgac cgtcgccgtg gccagccagc ggtaggcggg accatacttc
    12241 aggaacagcg aatcaacggt tcgagacatc gatgtccacc tcgaccatca tccccggctt
    12301 gagcaggcca tcctgctgct cgacgctgat cttgaccgga atgcgctggg tgatcttggt
    12361 gaagttgccg ctggggttgg ggctgggaat cagtgcgaac tggctggtgg ccgcgctgcc
    12421 gaccagcagc accttgccac tgaaggtgcg tcccggatag gcatcgacgg tgatgatgac
    12481 cggctgcccc tgcctgatct tctggacttc ggtctccttg atgttggcgt tgatccagac
    12541 atcgcggctg tcatgcacca gcgcaaagcg ctggccgggg gcgacatatt caccctcgtt
    12601 gacgaaggtc tgatcgacca cgccagttac cgggctggtg atggtgtgct ttttcacttc
    12661 gctggcctgc cgctcccgct gtacctgaat gctttcacgg ttgacccgca gccgctggat
    12721 ctgcttctcc agcacatcga cctgggcggc gttggcccgg ctttcggaga gtgccgccga
    12781 tgacgaggtg gccttggccc tgaccacatt gaggttctcg gccgcctgct tggctgcggt
    12841 ctgcgcctgt tccaactgcc gttgcgagac cagcttctgc gcggccagcg agacggttcg
    12901 acgcagctca tcctcggcct gcttcttgct cagctccgcc tccttgaccg aggcgccggc
    12961 ggcatcgacg ttggcaccgg cactttgcat ccggctctcg agctgatgtt gcagcacctg
    13021 cttcgaggtc tccatctctt cgatctgggc atcgagcttc tgcaattcga gcgacagctc
    13081 gctgagcagc agctccgccc tggtcgaatc gagcttggcc accatctggc cttcgttcac
    13141 cgtcatgccc tcatcgagcg gaaagctctc cagccgtccc ggcaattcgc tgctgacgat
    13201 gtagagcgtc gagcgaaggc gggcatcgat ctcatagaca tgggtcgagc ggtgataaat
    13261 ccattggccg aaaaagatca gcgcacccag cagcgcgatg gtcagcgctg cccgcacaat
    13321 gggttttttc atcaaagagg gctttttggg tgcggaggcc tcgagttgca gggtctggcc
    13381 aaggttggcg ttcatcggtt tttcggctcc attttcagtt gaatcttgcg cagcagttcg
    13441 gtgccgatgc gcagctcctg cggatcgatc tcaccgagca gttcgcggcg cagttgcgcg
    13501 gcgatgattt ccagctcagc cagaatcggc agcgccttgt cgcgcaggtg aacggtcttg
    13561 aagcggcgat cctgcggatg gggccgccgc tccacccatt cggccgcttc aaggcgatcg
    13621 agcagcgaga ccagggtggc ggcctcgatg ccgacccgcc cggccagatc gctctgggtc
    13681 agcggttcgc gtgcgcgttt gatgttcgac agcacccgcc actgcgcggc actgatgccg
    13741 gtgggtttga tgcgctggct gaggtgaagc cgccagaggc tcgaagtctc ggtgagcagc
    13801 gagacgaagc ggtcgatgtc gtcggtagaa gatggcatgg ctaatagata acaaaataaa
    13861 tggttagctt accaaccgta agaagtctag tcattgatag agctgattgc aacgccattt
    13921 tcatgcagcc gttgagtcag gcgtgcctgc ccggatggat gccatctcgt ataatggcca
    13981 tgagcgcgga tgtacagaac cgaaagcgga ggcatccatg caccaaccca tttccattca
    14041 aggacttgac gccagatgag tgagcagatc acccaccgcc ccaaccactt catccgccag
    14101 atcgtcgagg ccgatctggc gcgtggcgcc aatggcgggc aactggtgac ccgctttcct
    14161 ccggagccca acggttacct gcacattggc cacgccaaat cgatctgcct caacttcgga
    14221 ctggctgaag actatccggg cggacgctgc catctgcgct tcgacgacac caacccctgc
    14281 aaggaggagc tggaatatgt cgaggcaatc cagcgtgacg tgaagtggct cggcttcgac
    14341 tggcacggtc cggtccgctt tgcctcggac tacttcgagc aactctatgg gtatgccgag
    14401 gagctggtcg gcaaggggtt ggcctatgtc gacagcctgt cgagtgaaga gatgcgcagc
    14461 caccgcggca ccctgaccga gcccggccgc gacagccccg atcgctcacg cagcatcgag
    14521 gagaatctcg atctgctgcg gcggatgcgc gccggtgaat ttgccgacgg cgagcgggtg
    14581 ctgcgcgcca agatcgacat ggcctcgccc aacatcaacc tgcgcgatcc ggtcatctat
    14641 cgcgtgcgcc acgccagcca ccatcagacc ggcgacgcct ggtgcatcta cccgatgtac
    14701 gactacaccc attgcatctc ggacgcgctc gaagggatca cccactccct ctgtaccctc
    14761 gaattcgagg accatcgacc gctctacgac tggtttctcg atcatctcag tgtgccgtgc
    14821 catccgcagc agatcgaatt ctcgcgcctc gacctgcaat acaccctgac cagcaagcgc
    14881 aagctgcagc agctggtcga agagggcaag gtcagcggct gggacgaccc gcgcctgccc
    14941 accatcgccg gcctgcggcg gcgtggctac accccggccg ccattcgcga cttctgccgg
    15001 cgcatcggcg tcaccaaggc cgacaacagc gtggagatgg agctgctcga aagctgcatc
    15061 cgtgatgacc tcgatcacaa cgcgccacgg cggatggcgg tgctcgatcc gctcaagctg
    15121 gtgatcgaaa actacccgga tcagatcgag tggctgcagg tacccaacca tccgcagcac
    15181 gaagcgctgg gcagccgcaa gctccctttc gggcgagaga tctacatcga tcgggccgac
    15241 tttcgtgaaa cggcgggcaa ggagttcaag cggctggtca gcggtggcga ggtgcgactg
    15301 cgccatggct atgtgattcg ctgcgaccag gtgctgcgtg acgacagcgg cgagattcgc
    15361 gaactgcgct gcagccatga tccgcagacc ctgggcaagg cccccgaagg acgcaaggtg
    15421 aagggggtga tccactgggt cgaggcgagc catgccatcg ccgccgaact gcggctctac
    15481 cagcggctct tcgtcgacgc caatccggcc gcggccgaca actttctcga cagcctcaat
    15541 cccgattcgc tcaccatcgt gcagggcttt gtcgagcccg aactggccac ggcgcaggtc
    15601 gggcagggct accagttcga gcgcgagggc tacttctgtc tcgaccccga cagccagccg
    15661 cagcggctga tcttcaaccg caccgtggcg ctcaaggact ccttcgccag gatcgatcag
    15721 ccggcagggc gctgactgcg cgctatgcct gcgtgtcgat caactggccg cgacggccct
    15781 gcggggtcgg ccgtggcaat gcagagcggc gccgatcctt gcggatgcgg cgaccgttgt
    15841 tggcgcgctg gccgacattc agcatgcgcc gatcctgtgc gctgatccgg cgatccggct
    15901 ggcgccggcg gtcgatgcgc cgctcacctt catcggtcag atagaagcgg accggttcga
    15961 ccgggttcac ccggcggttt tcggtggggg cgttgttctc gaccggtgtg cggatcggcc
    16021 cgggtgcatc gatgcggatc atgtcgacct gacctcgacg aatcttcttg ccagaaagtc
    16081 tagccctccc agcgcaccag ctcctcgagg gtggcgcgct cggtgcggaa ctggttggcc
    16141 ggatggctcg gatcggcgta accgaaggag acgccgcaga ggatgcgctg ctcgggcgca
    16201 atcgcgaagt gctcacgaat gaagtcgctg taggcggcaa tggcagcctg ggcgatggtg
    16261 cccaccccct gattggtggc gctcaacaga aagctctgca caaagagtcc gcaatcgagc
    16321 gcgccatagc tgcccagcga ctcatggctg gtcacgatca tcacatgtgg cgcctcgaac
    16381 agccgaaagt tcttcagcgt ctgcgcgaag gaggcctgcc gatcaccatc ggcaatgccg
    16441 aggctggcat agagctgcat cgcacagctc ttgcgccggt cgtagtgggc gccgtggtac
    16501 ttgaccggaa acggaatgtc cgggttggcg ctcttgccag aattgacatg ggcccacagt
    16561 gcctcgcgaa agcggtcggt cgccgcccct tcggtgatga tcaactgcca cggctgggtg
    16621 ttgcaccagg aggccgactt gagcgcatcg tccagaatgc gctcgatcaa cgcgcggggc
    16681 agggcgtcgg gccgaaaggc gcgcacgctg tagcgctggg cgatcacctc ggaaaagtcc
    16741 atgagcggtc ctccatgaga ttttattgat tattggctgg tggcccatgg tagcactgga
    16801 gcaggcgctc atcatccgct tccgcgcctt gggccggata ccggcgccgc tcctgctgca
    16861 ctcggacaat gatctggtct tcaccagccg gcgtcacacg cgactggtgc gcagccgtgg
    16921 cctgcaacag gaattcatca cgcccactga ccacagcaga acggcatggt ggaacgggtc
    16981 atccggacac tgaaggagca atgcgtgcat ctcctgcgca tgccatttcg atcagtgcca
    17041 gttcagcccg ccgccagccg ctgcatcagc cagttcaccg cctgttccgg aggcagttcg
    17101 gtggcgcttg gggcgtcgcg ctgctggcat tcgacgaccc cctgggccag gccacggtca
    17161 ccgatgacga tgcggtgcgg caggccgatc agttcgcagt cggcgagctt gacaccgggg
    17221 cgctcgttgc ggtcatcgag cagcacctct acgcccagct cagcgagctg ggcgtagagg
    17281 cggtcggtca gttgccgcac cgcttcggat ttctggtagt tgagcgggat cagcgcgacc
    17341 gtgaacgggg cgatggcctg tggccagagg atgccgcgcg cgtcgtggtt ctgttcgatg
    17401 gcggcggcca cgacgcggga gacgccgatg ccgtagcagc ccatctccat gacgatggcg
    17461 cggccattgg ggtcgagcac gctggcgttc atcgccgcgc tgtacttggt gccgagctga
    17521 aagatgtggc cgacctcgat gccgcgtttg atcagcagtc ggccccggcc atcggggctg
    17581 gggtcgccct cttcgacacg gcgcaggtcg acgagggtga tgccggggca gtcgcgctgc
    17641 cagttggcgc cgctcaggtg gtggccgtct tcgttggcgc cgcagacgaa gctgtcgagc
    17701 agggcggccg agcggtcgac cagggtggtg atcggcagtc cgatcgggcc gatcgagccg
    17761 ggtccgcagc cgagcagtgc gcggatgcgc gcctcgctgg ccagcgtcag cggtgcggcg
    17821 acgccaggca gtttggcggc cttgatctcg gagaggcggt ggtcgccgcg cagcaccagc
    17881 gccaccagcg gggcgggctg ttcgtccgag gtggcttcgc cctcgaccac cagggtcttg
    17941 acggtccgac tcggcgccac gccgaggaac tggcagagct cggcgatgct cgactggccg
    18001 ggggtggcga tgcggctcag tgccgctgcc ggcaactgct gcgcgggcgg cacggcgacg
    18061 gcttcggcca gttcgacatt ggcggcgtag tcgctgtcgc tgctgaaggc aatctcatct
    18121 tcgccggagc tggccagcac atggaactcg tgcgaggcgc tgccaccgat gctgccggtg
    18181 tcggccagca cggcacgaaa ttgcagtccc agccgggtga agatgcgtga gtaggtctgg
    18241 tgcatcagtg cgtaggtggc ctgcagtgat gcgctgtcgg catgaaagga gtaggcatcc
    18301 ttcatgatga actcgcgcga gcgcatgatg ccgaaacggg ggcgcacctc gtcgcggaac
    18361 ttggtctgga tctgatagaa gttggccggg agctgccggt agctcttgat ctcgttgcgg
    18421 atcaggtcgg tgatcacctc ttcgtgggtc ggccccaggc agaagtcgcg ctggtgacgg
    18481 tcctggatgc gcagcagttc ggcgccatac tgctcccagc ggcccgactc ctgccacagc
    18541 tcggctggct gcaccaccgg catcagcagc tcctgggcgc cggagcggtc catctcctcg
    18601 cggacgatcc gctccacctt gcgcagcacc cgcagcccca tcggcagcca gctgtagagg
    18661 ccggaggcga gcttgcgcac cagtccggca cgcagcatca gctgatggct gatcacctcg
    18721 gcatcggccg gcgtctctct gagggtggaa accaatatct ggctggcgcg catgggtcgc
    18781 tccgtagcaa tggatgaaac gggccattct acgaaagcga tggcggacag gacagttcaa
    18841 tgaaagcgca gcgccgctca atggcagtgg ccatccggcg ccgactccgg tggcgtggcg
    18901 gctgccggct gtcgcagcgg ggtcaggttg ttgaggaatt gcagcgtcgc gttgcgccac
    18961 gagcaggctt tggcctgggc gacgcaggcc gccgggtcga gttgcagcgc ctgcggtatg
    19021 gcgagggcga gatcttcatg cagcacgccg gtcacgccgg gctgcaccac atccagcgga
    19081 ccggtcaccg ggtaggcggc caccggcacg ccgcaggcca tcgcctccag cagcaccagg
    19141 ccgaaggtgt cggtgcggct cggaaagacg aagacatctg ccgctgccag atgccggacc
    19201 aactcctcgc cctgcttgaa gccggtgaag tgtgcgtcgg gatggcgctc gcgcaggtcg
    19261 gccaatgccg ggccgtcgcc gaccacgacc ttgctgcccg gcaggtcgag ttcgagaaag
    19321 gcgtcgaggt tcttctcgac cgccacccgc cccacataga tcgcgatcgg tcgcggcagc
    19381 tgcagaaagt ccttgacgcc gggccggaac agcgcggtgt cgacgccacg gctccagcgc
    19441 accaggtggg tgaagccatg ctgttccagc accgcctgct gcgaggcggt ggccaccatg
    19501 gtgcgcgcgg ccgcggcgtg aaagcggcgc acggcagcgt agctccagcg cagcggaatc
    19561 ggcgtgcgca gcgccagata ctccgggaac tgggtgtggt aggcggtggt gaagctgaag
    19621 ccgtgccgca ggcagtggtg gcgggccgcc aatcccagcg gcccttcggt ggcgatgtgg
    19681 atggcatccg gcgccagctc atcgagcagc cgtgccaggc gtgggccggg ccagagcgcc
    19741 aggcgaatcg accggtaacc ggggcagggc agggtgcgga agcggtccgg accgatcacc
    19801 tcggccgtgt ggtcgagcgt ggtcagctcc gcgcaggtgc gccggagcgt ggtgacgacg
    19861 ccgttgatct gcggctccca ggcatccgag acgatggcga tcttcatgcc gccagcgccg
    19921 gcagcgccgt ttcggccttc agggcatgct gttggtcgga ccagtgcagc agttcgatct
    19981 tgccatcatc atgctcgacc agcgcggtgc agctctcgac ccagtcgccg cagttcaggt
    20041 agagcacctc gccgatggtg ctgatttcgg cgcggtgaat gtggccgcag accacgccat
    20101 cgacatcgtg ccggcgcgcc gccagcgcca ccgccgattc gaagttggag atgaagttga
    20161 ccgcattctt gaccctgtgc ttcagatggc cggccagcga ccagtagggc agtccacatc
    20221 tgctgcgcac gaagttgacc agccggttgg cacgcagcag cagcgcgtag gcctggctgc
    20281 cggccagtgc cagcagtcgg ctgcactgca ccacgctgtc gaactcgtcg ccatgcatca
    20341 ccagcaggcg gcggccatcg gcggtctggt gcaccacccg gccctgaatg gcgacgttgc
    20401 ccatctgcag gccatcatgc tcgcgcagca gttcgtcgtg gttgccgggc acgtagatca
    20461 cctcggtgcc atgcttggcc ttgccgagca gcgtgcgcac cacgttgttg tggctttgcg
    20521 gccagtagag gccgcgtcgc atctgccaga agtcgatgat gtcaccgacc agatagagcc
    20581 ggtcgcaggt gacgtggtgc agaaaatcga gcagaaagtc cgcctgacag ccggaaaagc
    20641 cgaggtggac atcggaaatc catacagtgc ggtagtgacg ggcccggagt gatgctggcg
    20701 acatgcaaac ctcctgcgag atgagttgcg gccagtcttc ggggtttttg tgaccgattg
    20761 attaacgacg aacgctcttt tttatcgctg atcaggttaa aaaatagaca gtccgcgggg
    20821 gtgagtctgc ttttttcatg cttccgaagt gggcggttgc gtccgccaat ttcctcgggt
    20881 ttcaacggcg gttcaggcct gtttgccctt cccgataggg tgaaacagag cacaaatttc
    20941 taatatgtca atgaaggttt attgagtatt ttcatgaaaa attaataaaa aacaccgttg
    21001 actctgcccg gcatgaggtc tatatttttt gcgtcgtcga tctgttgtcg atggcagaat
    21061 tttgaaacga agaaccgttc cattacctcg atcgagggag aatacgatga aaggaaannn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg gctatgggtt tggtgtcaag
    21241 accttcgtca cgccgaatct ctacctgcag gccgaggcgc agtggctcga tctgggttcg
    21301 gaatcctttg tcgacagcgt tggcgaccag tacaaggtca aggccagcgc gaactcggga
    21361 attttcagcg tcggctaccg gtactgattg atcctgcttc agcaaggccc actgttcagt
    21421 acagtgggcc ttttttatgc gcggcgtggc cgcgcgtgcc gatgacatcg gacaccgaac
    21481 cggctaaagt tgctgcgccg tgcgaccatc gcacgggcca gtgtgcctgt cagatgccga
    21541 cgaattcgac ccgaatcaat aaaacgacaa gaggagtcac gatgaaagca gccatcatgc
    21601 gtgaggtggg caagccactc tcgatcgaag aggtgcagat caacaagccg ggtccgcgcg
    21661 aggtgctgat ccgcactgcg gccgccgggg tgtgtcacag tgacctgcat ttcatcgacg
    21721 gccactaccc ctatccattg ccggtgatca tggggcacga gtcgtccggg gtggtcgagg
    21781 cggtcggttc cgacgtgacc tatgtgcagc ccggtgacca tgtcatcacc tgtctgtcgg
    21841 tcttctgcgg ccattgcgag gattgcctct ccggccacat gtcgcgctgc cagaatccgg
    21901 aggtgcgccg ttcgccgatg gggccgcagc cgttcagtca ggatggcaag ccgatcttcc
    21961 agttcctcaa cctctcctcc ttcgccgagc agatggtggt gcatgaacat gcgctggtga
    22021 agattcgcaa ggacatgccg cttgaccgcg ccgcgctgat tggttgcggt gtcaccatcg
    22081 gtgtcggtgc ggtgatccac accgccaagg tcgagccagg ttcgacggtg gcggtgatcg
    22141 gttgcggtgg cgtcgggctg tcggcgatca atggcgcggc cattgccggt gccggacgca
    22201 tcatcgccat cgacatgcag ccggccaagc tggagctggc caagcgcttc ggtgccaccg
    22261 atgtggtcaa tccgaaggat ggcgacgcgg tggcccgggt catcgagatg accggcggcg
    22321 gggtgcacta ctccttcgag gcgatcggtc tgaaggccac tgccgagcag gcgttcaaga
    22381 tgctgcgtcg tggcggtgcc gccaccatca tcggcatgat cccggtcggg gtgatgatcg
    22441 agctgcatgg cgccgagctg ctgaccgaga agaagatcca gggctccatc atgggctcca
    22501 accgcttccg catcgacatg ccgcgcttca tcgacttcta catggccggc aagctccacc
    22561 tcgacgagat ggtggcgcgg cgggtcaagc tcagcgaggt gaacgaatcg ctggccgcac
    22621 tcaagaccgg tgaaacggcg cgcaatgtga tcgtgttcga ccattgagtg tcgcatcgcc
    22681 ggatgcagca tgcgcaagat gggccggtga tccggcccat ctgcgttgag gtgaccctga
    22741 accgtttttg ccatgtccat tgatcctgca cacccatccg ctccggcgcc gatcgccctg
    22801 ttcgattccg gcatcggtgg cctgacggtg ttgcaggcgg tgcgccggcg gctgccggcc
    22861 gagtcgctgc tctatctggc cgacaccctg cacatgccct acggtgagaa atccgccgac
    22921 tggatcgtgg cgcgttcgct ggcgctgtgc gaactgctgc tggcacgggg ggccaaggcg
    22981 atcgtcgtgg cctgcaacac cgccaccagc gcggcggccg tgacgctgcg ccagcgtttc
    23041 gcggtgccga tcatcgcgct cgagccggcc atcaagccag cggccgcgct gacccgcagt
    23101 ggcgtcatcg gggtgctggc cacgccagcc acggcggaag ggacgcgcct ggccgggctg
    23161 atcgcgcagc acgcgcaggg tcaccgggtg ctggtgcagc cctgcccggg tctggtcgag
    23221 gcgatcgagt ccaaccagtc gcgtgcgctg atccgggcac tgctggccga gcggatcgcg
    23281 ccactgctcg atgccggcat cgacaccctg gtgctcggct gcacccacta tccgctggtg
    23341 gccgaagaga ttcgcgcgct ggtcgggccc gacgtggtgt tgctggacac ggccgaagcc
    23401 gttgcgcggg agctggagcg ccgtctgtcg caggaacggt tgctgcgggt cgatgcccgc
    23461 gacagcggcg aattggcgct gcacaccagt ggtgatccgc tgcggttgca gcagcagttg
    23521 cggcactgga tggccgatct gggcgatgcc agggtggaag agtcagcctc cctcgatctg
    23581 ccggccgatc ggtcgccgat cacccgcagc ctctgagcgg cacacctcag ccgggacggc
    23641 catcgatgcg tggccgcagc agaatcggca gatagaccac gacaaatgcc gcgaaggcgc
    23701 tgctccacag cagaccggaa agcagcagcc agcgggagtc gagcccacag gcggccacga
    23761 tgacccgcaa cagggcggcg ctggccagca gcatgaaggc gacccgggtc aacggcgcgg
    23821 cggcgatcag gcgtccgctg tggccgagcg cgacccggct catcatgccc agtgtcagca
    23881 ggccgatggc gcccacggtc agcgtgtggc tggcggcgga actggagagg ccgccgagca
    23941 ggctgaccgc actgagcaga tagccgatgc tgatcgcgcc gtagcccaga tgcagcaccc
    24001 agagcatcgg ttgccgccag atgcggcggt catgccaacc ggccagccgc atgccatgca
    24061 gcagtgccgc gctgccggcc agcgtcgcca gcagcgcgga gggcagcggc agcggatgca
    24121 gcagggcgaa gagccaactg ctccagaggg cgccgcgttc gacccactcc cgatgcactg
    24181 gctgcatgcc gggcagggcg ttgcggctga agagcggaat cacccgtccg ccaaagatca
    24241 ccagcagcag caccagcaga tcgaccatca gccgctggcc gatcaggctg ctgcctggcc
    24301 agtcggccag ccgctcgcca tggatcagca ggttggcagc ggccatcagc agcaacagcg
    24361 gtggaaacac caggttgtgc ggcttgcggt cctgcagcag cggaatggcg atgacagtgg
    24421 ccagcagcgg cagaaaggcc agatcggtca gcgcgatcag ccagtgtggc agcagggtgt
    24481 cgcaccaggg cagcagtcgg cctgccagcc acagcagcgc cagcgcggcc agtggccagc
    24541 ggaccggggt cgggcgaccg gtccagttgc gcaccgcggt cagcagaaag ccggccacca
    24601 ccgccacggc atagccgaac agcatctcat ggacatgcca gccgagggtt gggtagtagc
    24661 cagcgctggc gggccagtgg aattgcagca tggtcagcca cagcagcagt tgcaccaccg
    24721 cggcgatgcc ggccaaggcg aaaaaggggc gaaaacccag ctcgaacagt gccaggcgcg
    24781 gtggctgtgg tgctggagcc tcttgcagtg cgattttcat cgacaccccc tgggtgggag
    24841 aacagcggtg tgcagtcgtt atcgcgccac gcggccgggc atgtcaagct tgagcagtgc
    24901 agtgggcagc atggtcagca gaaagatcag ggtgatgccg aggaaggtgt cgcggtaggc
    24961 gaaggtggcc gcctggctgc cgatcatctg gccgaggtat ccgatcgccg caggcaactg
    25021 ctgtgcctcg gggaggccgt tggcggccag cagcgcacgc accttgttca gcagttcgag
    25081 ggtgaccaca ttgccgctgg tctgggtggt ggcaaaggcg tcatagtaaa agctgatgcg
    25141 atcctgcagg gcgacggtca gcaggttgac gccaaaggcg ccacccagtt ggcggatgaa
    25201 gttggttgct cctgaaccct ggctcatcaa ccggaacggc agcacccgca gtgcgccgga
    25261 gttgaggctg ggaaagatcg ccgccagacc gacccggccg atcagcaccc accagatcag
    25321 cgtgaagaag gaggtgttga tgtcgatgtt ggcgagcagc agcgacgaaa ccgagaaggt
    25381 gaagaggccg aaccagatca tcagccgcgc atcgatcttg tcgctgagga aaccactgac
    25441 cgggttgagc gcggccatgg cgaggccagc cggcatcagc agcagtccgg aggtggttgg
    25501 cgtgaccccg gccacggtct gcaggaacag tggcaccaga taggtcgagc caaacagccc
    25561 ggcgccaagg atgaaggaga tgccggccgc agcggcaaaa cggggttcgc gaaaaatctc
    25621 cagattgagc atcggatggg gggagtggtg ctcccaggcg atgaaggcaa cgcccgagac
    25681 cagcgaaacc gccagcaggg tcagaatata gtcggagttc cagccataac gctgaccgtt
    25741 ggccagtgcg gtcagcagcg tgaagacgaa gatcgccagc aataccagtc cgttccagtc
    25801 gaacggagtg cgcggcccgc tgccctcgcg tgccggcatg aagagaatgc cgagcggaaa
    25861 cgacagcagt gacagcggca cctggatgtg gaagacgtaa tgccaactga aggtgtcgac
    25921 cagcagtcca ccgatcgtcg gccccatggc cggggccaat accacgccga cactgaagat
    25981 gcccatcgcc atgccgcgct tgtcgagcgg gaacacctgg gtgatcagca gcatcgacag
    26041 cgggcccatc aggccgccgg cgatgccctg cacgatccgg gagatgatca tcacctcagc
    26101 ggtcggtgca aagccgccca tcagcgatcc gaccaggaag atgcccatcg ccgccagata
    26161 ggtgaagcgc attccatagg cctcgaccga ccaggcggtc agcagcatga agccggtggt
    26221 ggcagccagg tagccggtcg agacccactg tgcctggtcg ctgctcatgc cgaaggtccc
    26281 catgatgtcg gggatcgcga cattgatgat ggtgccggtc agcagcgtgg tgaaggaggc
    26341 gagcatcacc gtgaagacgg cataccagcg gtagctgggg ccatattcgg caaacagcgc
    26401 caatatcctg gggccggcca tcaggccggt cgggaccggg tggcgaccct cagcggactt
    26461 caatcgcaac ctccaccatc atgccggggc gcagcagatc accttcgacc tgatcgacgg
    26521 cgatgcggat cggaatgcgc tgggtgatct tggtgaagtt gccgctgggg ttggcgttgg
    26581 gcagcagcgc aaactggctg atggcggcat tgccgatgcg gaccaccttg ccttcgggcg
    26641 ccagctccgg atagccatcg acggtgatgc gcgcctgctg gccgagcctg aggtggtcga
    26701 ggtcggtctc cttgatgttg gcatcgaccc agatctgatc aggggcatgc atcagcagga
    26761 tgcgctgtcc ggtgccgatg aactccccat cgtgggtgaa ggtctcgtcg atcaccccgt
    26821 cgatcgggct cagaacggtg cggtcatcga ggtcgatctg ctggcgctgg cggttggcct
    26881 gcaactgctc cagctggtgc tgggttcgga ggatggtctg ctccagttgc cgcaactggt
    26941 cgcggctggc ctcggcctcc agcagagtcg aatgggcagc ggccagttcg gcccgactgc
    27001 gctcgacggc ctggcgggcg gtgtcgagcg cggtctgctt gctgtcccac tcgcgttcgg
    27061 ccagcagccg ctgctggcgc agtgtgcggg cacgctccag ctcgctcttg gcgaagttgt
    27121 agttggattg ggcggtggcc agcgtggtct gcaccgcctc ctgtcgggag cgggccgcat
    27181 ccagacggtt ggtggtctgc cgatcgagca ggtcgtgctc cagtttctgc cgggcgaggt
    27241 cggtttcggc gatggcgatc tgcgcttcga tctcgcgcag acgcagtgtg gtgtcacggg
    27301 catccagcgt gagcagcggc tgatccttct tcacccggtc gctggcactg accatcagat
    27361 cggtgaccca acccggagca cggctcgaaa ccgacaccat gctggcaccg atgcgtgcat
    27421 cggagatgta gacatgggtg tagcgctggt agagccagta accggctccg atcagacagg
    27481 cgacggcaaa gagtcccagc agcagggtgc gtcgaccctg ggttggcgtg agcgtggcgg
    27541 agctggcgtc attcatgatg gttggattgc tccctggcgg gtttgatggt gttcaggttg
    27601 tgcagcagac gctcgaacag gtcgatgcac agcgccatct cctgttccga aacgccatcg
    27661 agcagttgcg cgcgcaacgc ttcggcggct tgatggaatt cggtgagcac cggataggcg
    27721 gcatcggtga ggtggatggt cttgccgcgg cggtcgcggt gggtggagcg acgttcgatc
    27781 agcccctgtc gttgcaggtc atcgagcagt ctgaccaggg ttggaccctc gatgccgatg
    27841 tagcgggcga gatctttctg caacaggcca tcgccgccgc gggtcaggta gaccagcgcg
    27901 ttccacttcg actgggtcag gccggtgtgc ttcaactgct gatcgagccg catgcgccag
    27961 cagcgggcca gatgtaccag cttgaatccg aacagttcac gggccgagga gctttcttgc
    28021 attgttattg ttctgaccat ccatgtgctt gcagatccag acagatagca agcaaattag
    28081 tagcgtgcta attctggaaa ccgcagccga aggtgtcaat atgcaatttc atccgcaagg
    28141 tggatggaac tgaatgtcgc gtgacgccag cccagcgagc cgcgggcgcc cgtcggccgt
    28201 gcagtccgtt ctggccgatg tggaggctgc cggagagcgc tgggcagggg cagcactgcg
    28261 cctctgcgtg gtggcgtacc atgcaaagcg ccaaggaacg ctcagctcac ccggctattg
    28321 cagcacaaag cccggatagc cgttggcact ctcctggtca caccgctcgc ggtaggccgg
    28381 agcgccgccg acatagacga cgatgcgtgg cttgtccttg cccggcacat tggtgttgac
    28441 gccggtgaac caggagttga ccttggtgaa cagcgtgtat tcggcaatct cgttgacatg
    28501 ctgggtccag ctccgctcgg cctcttcgct ggggacgacg ctctgcaact ggtggctgcg
    28561 caggtggcca atcactccgg agacccactc gacattctgt tcgatgcagc gcgggatgtt
    28621 gcagagggtg gcggtgttgt gcggtccgac cagcgtgaac agattgggga agccgacgct
    28681 ctgcaatccg aggtaggtgc gcgggccatc ctgccatttg tccttcagtt tgagaccgtt
    28741 gacgccgcgg atgtcgatgc ggtcgagcgc gccggtgatc gcatcgaagc cggtggcgta
    28801 gatgatgatg tcgaattcac gctcgcggtc gctggtcttg atgccctggg gggtgatgcg
    28861 ttcgatcggc gtttcgatca gcgagaccag ttcgacgttc ggctggttgt agacctcgta
    28921 gtagtgagtc tcctgcggca cccggcgggt gccgaagcca tggtcggtcg gaatcagttt
    28981 ttcggcggtc tttggatcct tgacccgctg gcgaatcttt ttggcgatga agtcgctgat
    29041 ggtcctgttg gcctcgggcg tcatcaggat gtccatgaag ttgccgatcc agatggcgaa
    29101 gccgggctgc tggtagaggt gctcgaaaaa ggcttcacgc tcctcgggtg acacttcgag
    29161 cgcgctgcgc ggatcggact tgtgcaggaa gcagccgttg gtctcacggc actgctggaa
    29221 gatttgcggg tagctcgcct tgatctggcg ctgctcctcg ggcgtgatcg gacggttgtt
    29281 gagtggcgtg caccagttgg gacggcgctg gaagacggtg agctgcttcg cgctcttggc
    29341 cacctcctgg atcacctgca cgccggtggc gccggtgccg atcaccgcca cccgcttgcc
    29401 ggtgaagtcg acctcgtggt gcggccagtt cgcggtgtgg caggattcgc ccttgaagct
    29461 gtcgatgccc tcgatcgccg gcagggtcgc tgccgagagc ggaccgaccg cggtgatcag
    29521 aaagcgtgcg cgggcctggg tgccggtgtc ggtggtgacg gtccagcagc tgtccgcttc
    29581 gctgtagtgg gccgcggtga cccgggtgtt gaactggatg tcccggcgca gatcgaactt
    29641 gtcggcgaca tggttgagat agcgcagcgt ctctgactgg ggcgagaagt gctcactcca
    29701 ctcccactcc tgcagcagct cttcggaaaa tgagtagccg tagctgtagc tctcggagtc
    29761 gaagcgtgcg ccaggatagc ggttccagta ccaggtgccg ccgacaccat cgccggtctc
    29821 gaacacccgc accgagcagc ccagctcgcg cagtttgatc agttggtaca tgccggcgat
    29881 gccggcgccg atgatgatgg cgtcatactg ttcggcaggt tcaagtttga cggcggtggc
    29941 catgagattc gactccgttc atgattatta ttgagtgggc agacggcccg ccggacaggc
    30001 tagctgccag gcgggatgtc ggcaattgat ccatgtcaag caggcgcaaa ctaccaccaa
    30061 agcgacaggg cagccagccg tctgacgcga ccggtcacca catcaggtca tcgggaatcg
    30121 ggtggtcggc gtaggggtcg gtctcattcg cgtcctccgc agcgctgctg ggcggcgcgt
    30181 tcagcaccac gacaacgccc ggatcgcgct gggcgatttt ctgcgccgtg ccgaccggca
    30241 ccagctcatg gcgctcgtcg agccggacca cggccagcgt gccgcgaatc aactgctcgc
    30301 gctgctgcgc gtgcagcacg atcttcttga tcttgccatg gtcgacgaac tggtgactga
    30361 ccgtgccacc gctgcggtcg atcctctggg tctcgatcaa ctggcgaatc tgggcggcca
    30421 gagccttctg ctcgcgcgcc tgctgctgtt cgaggttgcg ttgccggtcg ctttcggcct
    30481 tctgctgctg tgcctgccgg atctgttccg ccgtcgggtc ggcgttgccc ggctgctggc
    30541 tggcctggta cttctccttg gcggccttct tgacctggtt gctgttgacc agtcccgcct
    30601 tgagcagctg ttcttgcagt gggctgacca tcgtgtgcgt gcctcaggtg ttggagggtg
    30661 gttcgcagag cagttgggtc agcgcgtcca gcagggcgtc attctcggca ggtgtgccga
    30721 cggtgatgcg caggcagtcg cgcagcaggg gatggccgcc atccagacat ttgatcagga
    30781 tgcggtgacg cgccttcagc agccgctgca accggggcgc ctcgccagcc ggcacccgca
    30841 gcaggatgaa gttggcctcg ctggcatagg gggtgatgcc gggcaggctc tgcagcgcgg
    30901 cgaacaggcg gctgcgttcg cggcgcaact gttggctctg ttcggtcagc agcgacgcat
    30961 gttcgagggc aaagcaggcg ctggcctggg tcaggctgtt gatgttgtag ggcagccgca
    31021 ggctgtcgag ccgttcgatc agcgccggag cgccgatcag caatccaagc cgcagtccgg
    31081 ccagaccgat cttggagagg gtgcgcatca ccagcaggtg cggaaactca tcgagcagtg
    31141 gcagatgctg ggtgtcggtg aaggcgcagt aggcctcgtc gatcaccacg acgccgggtg
    31201 ccgcctcgat gacggcccgc agcgtggccg ggtcgaagag gttgccggtc gggttgttcg
    31261 gctgggcgat gaagatcact gccggctggc gctcggcaat cgccgccagc atcgcggtgc
    31321 ggtcgagttc gaactgctcg gccaatgcaa cgccgcaata ggccatgccg gcctgacggg
    31381 cgatcaggct gtacatcacg aagcccggct cgggtgccag caggcagtga ccggtgccac
    31441 cgaacagcag tgccagcagc aggatcagct catcggagcc attgcccagc agcagcgcat
    31501 ggtgctcggc aatggcgaag tgccgccgca gcgcctgctt gactgcgctg ccctgcggat
    31561 cggggtagcg gttgagttcg acgctgctca gccgttcgag ccaggccgtg gtgagtgtgc
    31621 cgggccaggg gtaggggttc tccatggcat cgagcttgat gcagccgctg gcatcggcga
    31681 catggtaggg ggcgagcgag cgcagttcgg gccggatcca ctgttcgagg cgggtgtgca
    31741 gttcttcagg tggtggcgac ggctcggtca tggcgtcgtg ctccccggtt cgcgcagccg
    31801 gcattcggca gcggcggcgt gggcttccag cccttcgaga cgggccaggg tgatggcggt
    31861 gtcggcgagc cgcgcggcgg cctgcgagga gcagccgatg atcgagctgc gcttctggaa
    31921 gtcgtagacc ccgagcggcg aggagaagcg ggcggtgccg gaggtgggca gcacatggtt
    31981 ggggccggcg cagtagtcgc cgagcgattc gcaggcatgg cggccaagaa agatggcgcc
    32041 ggcatggcga atcgatggca gcagtgcttc ggggtcggcg accgacagct ccagatgttc
    32101 cggtgcaatg tggttgatca gctcgacccc ctgttgcagg tcggtcacct gcagcagcag
    32161 gccgcgtttt tgcagtgcgg cggcgatgat ggcggcgcgt ggctgtgccg gcagcagtcg
    32221 ggccatggtg tcggtgaccc gatcgaggta ggccgcatcg gggcagatca ggatcgattg
    32281 cgcctcttcg tcatgttcgg cctgggcgaa caggtccatc accacccact ccgctggggt
    32341 cttgccatcg cagagaatca gcacttcgga aggaccggcg atcatgtcga tgccgacctg
    32401 gccatagagc agccgtttgg cggtggcgac atagcggttg ccgggaccga cgatcttgtc
    32461 gacccgcggc acgctggccg tgccgtaggc cagcgcggcg accgcctggg cgccaccgat
    32521 ggtgaagagc cggtcgacgc cggcgatcgc ggccgcggcc agcacccagg ggttgagggt
    32581 gccatcgggc gtcgggctga ccatgatgat ctcgccgacg ccagccacct tggccggaat
    32641 cacattcatc agcaccgagg aggggtaggc ggcccggcca cccgggacgt agaccccgac
    32701 ccgatcgagc gggcgcacct cctgccccag cgtgaagccg gcttcatcct cgatgcgcca
    32761 gctcggctgc cgttgcagcg cgtgaaaggc gcgaatgcgt tgggcggcat gttgcagtgc
    32821 cgccacgccc gcggcgggca ggttttgcag ctgggcatcg agcctgccgc agtcgagctc
    32881 cagtgcagcc atcgtttgcg cggggtggcg atcgaagcgc tgggtcagcg ccagcagagc
    32941 cgcatcgcct tcgcgtcgca cctgctcgat caggctgcgc acctgattgg tgtggagcag
    33001 atcctcctcc atctcgaagc cgagccggct gtcgagctgc tgcatgaagt cggtggcgcg
    33061 gctgtcgatc cgggtgatca gcgtcgggtt cgtcatggct gacccgcctg gaggcggttg
    33121 cgatcggcca ccgcggcgga aagctgctcg atcagcgcgc tgaccgcgcg gtgcttgacc
    33181 ttcatcgacg ccttgttgac gatcagccgc gagctgatgt cggcgatcag ttccagcggt
    33241 gccagcccgt tggcctgcaa cgtcttgccg gtgtcgacga tgtcgacgat ctggtcggcc
    33301 agtccgacca gcggcgccag ctccatgccg ccgtagagtt tgatcagctc gatctgttca
    33361 ccgcgttcgc catagaagcg cttggccacg ttgacgaact tggtggccac ccgcagccgg
    33421 ccacgccggg ggggcgcgac caccgggccg gccgtcatca gccggcagcg ggcaatgccg
    33481 aggtcgagtg gctcgtagag tccgctgctg ccctgttcga gcagcatgtc cttgccggcc
    33541 acgccgaggt cggccgcgcc atgccgcaca taggtcggca catcggtacc gcgcatcacc
    33601 agcaggcgca cgtcgggctg gttggtggcg aagatcagct tgcggctctc ggcgatcgag
    33661 tcgagcggct cgatgccggc ggcccgcaac agcggcaggc attcgtcgag gatgcggccc
    33721 ttggtgaggg cgatggtcag tgggctcatg tcatggctcg aatacggcga acgggtctca
    33781 ggcggcgacg cgcctgatct gggcgccgag ctgaagcagt ttctcttcga tgcactcata
    33841 gccgcggtcg atgtggtaga tgcgttcgac ctcggtctgc ccgctcgcca ccagacccgc
    33901 cagcaccagt gaggccgagg cgcgcaggtc ggtcgccatc accggcgccg cgcgcaggtg
    33961 gtcgaccccg ctgacgatgg cggtgttgcc ttcgagcgcg atgttggcgc ccagccggtt
    34021 cagctcatgc acatgcatga agcggttctc gaagacggtc tcgatgatgg tgccggtgcc
    34081 gcgggcgatt gaattgagca ccgtgaactg cgcctgcatg tcggtcggga aggccggata
    34141 gggggcggtg cgcagattga ccgcccgtgg ccgctccccc ttcatgtcga gctggatcca
    34201 gttcggcccg gtctcgatgg ccgcaccggc ctcttcgagc ttgtgcagaa ccgcttcgag
    34261 aatcaccggt gcggtgtcct tcagtcggat ccgcccaccg gttgctgcgg cggccaccag
    34321 ataggtgccg gtctcgatgc gatcgggcat gacatcgaag cggcagccgt gcaaccgctc
    34381 caccccttcg atgaccaggg tgtcactgcc ctggccgctg actttggccc ccatcgcatt
    34441 caggcagttt gccagatcga ccacttcggg ctcgcgggcg gcgttttcga tcactgttcg
    34501 cccctcggcg agcaccgccg ccatcagcac gttttcggtg ccggtgaccg tgaccatgtc
    34561 catgaagatg tgggcacctt taaggcgtcc gtcgacccgg gccttgatgt agcccccttc
    34621 gaggtcgatc tgcgcgccca tcgtctcgag gccacggatg tgcatgtcga ccggacggct
    34681 gccgatggcg caaccaccgg gcagtgacac ctcggcctgc ccatggcggg ccagcagcgg
    34741 ccccagcacc agaatcgagg cgcgcatggt cttgaccagt tcataggggg cactgggggt
    34801 gtgcagggtg cgggtgttga tctcgacccg catctgctca tcgagcatca cctcgacccc
    34861 catgcagccg agcagctcga tcatcgtggt gatgtcgtgc agatggggca gattgccgat
    34921 ctccatcttc tcgtcacaca gcagggtcgc ggccagaatc ggcagcgccg agttcttggc
    34981 gccggaaatg cggatttctc cgtcgaggcg caccccgccg gtaatgatca acttgtccac
    35041 gatgcctctt gtttcattgc ggaaatctga atggatcggg cggcggtttc aggcgcgctg
    35101 ctgttcatcc ggggtgtagg ttttcatgct gatcgcatgc agcgcacccg aggcgatgtt
    35161 ggcctgcagg cagccataga cctgctgctg ccgcttgacc gggctcatgc cgctgaaccg
    35221 atcccaggtc accaccacct gaaagtggta gccgtcgcca tcgacctgtg cctggctgcc
    35281 gggaagcccg gtctcgatga gtgccttgat ctcgtccagt tgcatcgcgc tgtcaccgat
    35341 cgaaagtgaa gaatgggggt ggaatggtat agaaagcggg tgtcgaaggc gagcggccgg
    35401 cgcgctccag cggccgattt cgtgccggat tgacgcgggg caatggcagc gcggtttttg
    35461 cgctgttcag gtccacccat cggacatacc atgaaagagc ggcgctgacc tgccgcgaat
    35521 ccgacatcaa gcctgccgaa caggagagcg agttatgacc accacctgga ttctggttgc
    35581 cgagcgcagc cgcgcccgca ttttcacgct gcagaaccga gtggcgccac tgcaggagct
    35641 ggagagtctg gtgcatccac agtcccgcct caaggagagc gagctggtca gcgaccatgc
    35701 cggcaagctc agtgacaagg gacagcccgg cacccatgcc gcgccgtcag ccaccagtgc
    35761 caaggagaag gaggccgagc tgtttgcccg gcagctctgc caacggctgg aacagggccg
    35821 ggcacagcag cagctggagc ggctggtggt cattgcggca ccggattttc tcggtctgct
    35881 gcgtcagcag atgggcgcgg ccctgcgggc actggtcgtg gccgagctgg acaaaaatct
    35941 ggccatggcc tccgccgaac agattcgcag ccacctgcca gagcgcctcg ctcagtaaag
    36001 gcatcgtgtt gcagccgccc tggtgcaggc cggggtggca ccctccattg tttcgctggt
    36061 gacgcccgcg ggcatctgcc tggtttgtct gttcaatccg atcctttgcc agcggtggcg
    36121 ccccggcctt tgacctgccg tggcgcaaac ggcgagaatt gcctgctttg tgatcttttc
    36181 ggaccctgga tacgctcacg aggtgccgac agcgtctgag atgtccgaag cgatgcagtg
    36241 cagttcaacc gattccgcct gaccggcgac gaggcctctg acccgatgga tttcaccacc
    36301 accttcagcg aggatggcag cgagcaggtc gatttgcgcc tctataccga gcgcgcctac
    36361 ctcgactatt cgatgtatgt gattctcgac cgtgccctgc cgcatctgtg tgatggcatg
    36421 aagccggtgc agcggcgcat catctatgcg atgtcggaac tggggctggc cagtggcgcc
    36481 aagttcaaga agtcggcgcg cacgatcggc gatgtgatcg gcaagttcca tccccatggc
    36541 gacagcgcct gttacgaggc gatggtgctg atggcgcagc cctttgccta ccgctatccg
    36601 ctggtcgatg ggcagggcaa ctggggctcg accgatgacc ccaagtcgtt cgccgcgatg
    36661 cgctacaccg aggcacgcct gaccgcctat gccgagctgc tgctgggcga gctggagcag
    36721 ggcacggtcg actggttgcc caatttcgat ggcacgctgg aggagccggc gcgattgccg
    36781 gcccggctgc ccaacctgct gctcaatggt gccagtggca ttgcggtggg gatgaccacc
    36841 gacattccgc cgcacaacct gcgcgaggtg gctgcggcct gcgtgcggct gctcgatgaa
    36901 cccgataccg ccatcgagac gctctgcgag ctgattcccg ggccggattt ccccaccgag
    36961 gcggagatca tcactccgcc cgatgagctg cgcaaaatct acagcgaagg gcgcggcagc
    37021 gtgcgcctgc gcagccgtta cgaacaggag gagggcgagg tcatcatcaa ggcattgccc
    37081 catcaggtgt cgggcagccg ggtgatggag cagattgccg agcagatgca ggcgcgcaag
    37141 ctgccgatgg tggtcgatct gcgcgatgaa tcggaccatg aaaacccgac ccggctggtg
    37201 ctggtgctgc gttctgggcg ggtcgaggtc gatgcgctga tgaaccacct gtttgccacc
    37261 accgatctgg agcgcagcgt tcgggtcaac ttcaacctga tcggcctgga tggccggccg
    37321 caggtgatgg acatcaggca gctgctcggt gactggctgc agttccgtcg cgacaccgtg
    37381 cgccgtcggc tcaaccaccg gttggagcgg gtgctggagc ggctgcatgt gctcgagggg
    37441 cttttgatcg ctttcctcaa catcgatgag gtgatccgca tcatccgcaa cgaggatgag
    37501 cccaaaccgg cactgatcgc ccgttttgcg ctcagtgagc ggcaggccga ggcgattctg
    37561 gagctgcggc tgcgccactt ggccaggctc gaagagatga agatccgcgg cgaacaggcc
    37621 gcgctggccg aagagcgcga ccacctgcag gccctgctcg gttccgagac gctgctgacc
    37681 cggttgattc gtgacgagat cgagcgcgat gccgagcgct acggcgatgc ccgccgttcg
    37741 ccgctcgtgg cccgtggggc ggcccgccag ctcgatgaag aggcgctgct gccgaatgag
    37801 ccgatcacgg tgattctttc cagcaatggc tggattcgcg cggccaaggg gcatgagatc
    37861 gatccggccg cactgtcgtt ccgtactggt gacgggttgt tgcaagcggt gcgtggacgc
    37921 agtcagcagg agtcgatcct gctcgattcg accggtcggg tctatgccat ctccagccat
    37981 ggcctgccct cggcacgggg ccagggtgaa ccgctgaccg gccgactcgc gccgcccgcc
    38041 aacagccggt tcgtcgcgct gttgatgggc gaagccgggc agacatggct gatcgcttcc
    38101 gatgccggct acggcttcat cgtccgtcat gaggatctgc tgagccgcaa ccgggccggc
    38161 aaggcactgc tgacgctgcc ggccggcgcc caggtgttgc caccgcggcg ggtggctgat
    38221 ctggagagcg accggctggc gctggtcacc aacgatggcc ggctgctgat ctgcgcgctg
    38281 gccgagctgc cgcgactggc acgcggcaag ggcaatcgac tgatcgcgat tccggcacag
    38341 cgggcgcagg agcgcgagga gtacctgttg gcggccgaag tgctgggcgc gcaggatggg
    38401 ctggtgatcc atgccggcaa gcgccatctg acgctgaacc cggcggatct gatccactat
    38461 cagggcgagc gcggccggcg tgggctgaag ctgccgcgcg gtctgcaacg ggtggagcgc
    38521 atcgaacggc tggcacgcgg tggctgaacc gcgactgttt gcggcgcctt tttcaaattg
    38581 catggagtga cggtcggacg tggcaaagat tctgctgatt accctctcgg gcagtgacca
    38641 gcccggcatc accgcggcga tcaccgcggt gctggccccc tatgatgtgc aactgctcga
    38701 catcggtcag gcggtgatcc atgacaccct gtcgctcggt ctgctgatcg agctgcccac
    38761 cgccgccgaa tcgtcgccgg tgctgaagga tgtgctgttc tgcgcgcacg aactgggcgt
    38821 gcaggtgcgc ttcacgccgg tctcgctcga caactaccgc cgctgggtcg agcaccaggc
    38881 acgctcgcgc tacatcgtca cactgctggc gcgcaagatc agcgccctgc atctggcacg
    38941 ggtcacccgc attgtccgcg accatgagct caacatcgtc ggcatcaacc ggctctccgg
    39001 acgcatcgca ctcgatgccg accccgctcg cacccaggcc tgcgtcgaac tctccctgcg
    39061 cggcgaagcg gtcgatctgc cggggctgcg cgcgcagttt ctggccatcg ccaatgagct
    39121 cgatgtcgac atcgcctttc aggaggacaa cgtctttcgc cgcaaccgcc ggctggtcgc
    39181 cttcgacatg gattcgacgc tgatcgaggc cgaggtgatc gacgaactgg ccaaacgggc
    39241 cggggtgggg gccgaggtgg ccgcgatcac cgagcgggcg atgcgcggcg agctcgactt
    39301 caccgacagc ttcacccgcc gggtggcgct gctcaagggg ctgccggagt cggtgctggc
    39361 cgaagtggcc gatcaactgc cgctgaccga aggagcgcag cggctgatcg acaccctgcg
    39421 tcgactcggc tacaagacgg cgatcctctc cggcggcttc acctacttcg gtcgctggct
    39481 gcagcagcgg ctcggcatcg atcatgtcca tgccaatgag ctggagatcg aaaatggccg
    39541 ggtcaccggc cgggtgctgc cgccggtggt cgacgggctg cgcaaggccg aactgctgcg
    39601 gcagatcgcc gcgcaggagc ggatcagcct cgatcaggtg atcgccgtcg gcgat
//
LOCUS       DAORCQ010000010        38924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_519_length_38924_cov_5.990352, whole genome shotgun sequence.
ACCESSION   DAORCQ010000010 DAORCQ010000000
VERSION     DAORCQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 38924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38924
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_38924_cov_5.990352"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1453
                     /locus_tag="PKI23_01820"
     CDS             194..1453
                     /locus_tag="PKI23_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN35707.1"
                     /translation="MATETPAQSSLAALLRQRAFIFFWLARVCTASAFQMQGVVLGWQ
                     VYQLTGRALDLGLVGLCQFLPRVVLTPWAGHVADRFDRRRVTLLMQLLQGILLLLLCL
                     VSAQGAVNRELVLLLVALGGAARTFEVPATQALLSAVVPPLLLPRAVAISSSAMQAAT
                     LIGPVVAGLLYLLGATEAYGVTALLYVAAAAMMAAVRPLVHQDRSGPQSNFLASFMEG
                     IRFLRRQPMLLGAISLDMFAVLLGGATALLPIIADQLLQTGPVGLGLLRAAPAVGALA
                     MSFWLASHPLHAAGRALFIGVALFGLATLALGLSRSLPLSLAILLLLGAADMVSVVIR
                     QTLVQLHTPDEMRGRVSSVNAIFIGASNQLGEFESGLTAQWFGLVPSILLGGSGTLLV
                     VGLWMRWFPQLLQVRSLEEAMPAQNRP"
     gene            complement(1505..1999)
                     /locus_tag="PKI23_01825"
     CDS             complement(1505..1999)
                     /locus_tag="PKI23_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNN35708.1"
                     /translation="MKEKKSKGSAIDIGIDGADRQKIVDGLSALLADSYTLYLMTHNF
                     HWNVTGPHFNSLHQMFMVQYTEQWNALDLIAERIRALGHPAPGTYREFAKLASIEEVE
                     GVPAAMEMVRHLVAAQEATARTARKLLPIADAANDQPTIDLLTQRLNLHEKTAWMLRS
                     LLEA"
     gene            complement(2139..4904)
                     /locus_tag="PKI23_01830"
     CDS             complement(2139..4904)
                     /locus_tag="PKI23_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HNN35709.1"
                     /translation="MDSSTVETWSDAGVDRKALRQTVERFRRVNDARLERYRGSRNER
                     QRQLLDLLPLLFHLDHPLLPGAGSGPTPFGVAGYQPGREVLHAAQQLSRAFRYNPVSE
                     QQRDIDAIYLMGSVGSFACNASSDLDIWLCHRPGLDDAAVDRLRRKAQRIARWAAELD
                     LDLHFFVFDCDSFRRGEQSELSSESSGSAQHGLLLDEFYRTGLLLAGRYPIWWLTPPE
                     LELHHAEHAEWLRQRRFIDAREVIDFGSVARVPSEEFLGAGIWQLFKSIDAPYKSVLK
                     LLLIECYASQHSVNRALALDYKTAIYAGITDPDQLDPYVFLYRRLEAHLQEQHDPQRI
                     ELIRRCLYHKAEGGGTGWRRALMRRLTDEWGWDRGRLARLDRAQWSIERTIEEWRRLV
                     VELTFSYRALTRFARDRQLTNVMDARELQVLQRKLNTAFERRPGKIDLQPACLPLPFA
                     PRLHFKPRPASDAGWALFDQVDVATSPPIRQARQLGELIAWAHFNQLAGRSSRSTVEG
                     SLSERALQRLFAELERWQPMPLPPTPHQAFASVQQPLSSMLVINLDSETPSTLHASGL
                     ARISSRSDALGYSGLRQNLVRTIDSFILTSWGELLTQRHVGNAALIDTLVDHLNGHGM
                     ALERLPPPPVISCMTEGREMLISERIEQCFVAVRQHFAPHGSRTARYLFEIEKELFML
                     EWEGARVVQRRFGSMGALFGHLATPAAQFRTLQIDPMSLPQHDLHVVARLNQPNRIEL
                     LYRLQGQQIRIYIVDESGALFALAHPGSDPRQAVGPWLGFLERIDERRQRDGAPALAL
                     HCFELTGREPLATPRSFASLVSRLAYQGVEAVAEPAEGGESRFMLRVGDAEFSSRHDG
                     EALYGRVAEALLRQRRSGERYPAHLSDLDLGALGGPPPSTASYLHHKVRIEAAINRFF
                     MRQESVG"
     gene            complement(4958..6367)
                     /gene="argH"
                     /locus_tag="PKI23_01835"
     CDS             complement(4958..6367)
                     /gene="argH"
                     /locus_tag="PKI23_01835"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNN35710.1"
                     /translation="MNDASSTHQLWGGRFQQATDAFVQRFTASVGFDQRLALHDIRGS
                     LAHAEMLTRVGVLDNEEFRQISSGLGQIRQEIESGQFRWSIELEDVHMNIEERLTQLI
                     GAAGKKLHTGRSRNDQVATDIRLYLRDEIDQIERLINRFQAVLVDLAEREADTIMPGF
                     THLQSAQPVTFGHHMLAWYEMLERDRERLLDCRVRVNRMPLGAAALAGTTYPIDRTLT
                     AALLGFSAPAGNSLDAVSDRDFAIEFVSACALLMTHLSRFSEELILWSSAQFRFIELP
                     DRFCTGSSIMPQKKNPDVPELVRGKSGRVNGHLVALLTLMKGQPLAYNKDNQEDKEPL
                     FDTIDTVTDSLLAYTDMMAAIELNRPVLRAAAESGYATATDLADYLVKRGVPFRDAHH
                     IVGRAVQHAIGQQLALSELPLELLRQFCASIDEDVYQVLTPEGSVAARDHLGGTAPRQ
                     VREAVARARTGLAGRAAAR"
     gene            6458..7528
                     /locus_tag="PKI23_01840"
     CDS             6458..7528
                     /locus_tag="PKI23_01840"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNN35711.1"
                     /translation="MHRTTAKRPATAHPLPTSVESGLVPDFSRVGPLLFLLLLCELLV
                     IVLVLAASGVRQFDWQMLALWSLQVEWIALTSALLISRLRARLLRLEPAAAVSALYLL
                     VLAVVMVVTLCGQWFTTWAVAETTDWFDPYALATALVIGAITTGVALRYLYLSDRLRV
                     EQQAALTARLDALQARIRPHFLFNSLNSIAALISIDPLAAEELLVDLSELFRATLKEG
                     ERLVPLTREIELCQRYLAIEQRRLGERLRVVWQLAPHPDSARLPPLTLQPLLENAIYH
                     GVQPLLDGGEIHIRLSRRGERIEIEITNPLPAASAASEGHRIGLTNTRARLRNCFGDR
                     AEVQGSATDGGYRTLLSFPDQI"
     gene            7544..8320
                     /locus_tag="PKI23_01845"
     CDS             7544..8320
                     /locus_tag="PKI23_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNN35712.1"
                     /translation="MSERGQRGIDAVRRVMVVDDEPLARQRLASLVNDLEHYQVVAMA
                     GDGHAALELLALEAPEILLLDIRMPGLNGLDLAARLRDLPRPPAILFCTAYDHYAREA
                     FGVRAAGYLLKPVRRADLLQALEDACQLSRLQWAAIEQARARPVAGGGRSHLSIQRRH
                     GIDLIALDEVRAFIAEQKYVTVIHAAGEALIDEPLRALEQEFADRLVRIHRNALVALD
                     AVESLEATDSGHRLRLRGVERPLLVSRRHLTELKRRLRRV"
     gene            8396..9337
                     /gene="hemC"
                     /locus_tag="PKI23_01850"
     CDS             8396..9337
                     /gene="hemC"
                     /locus_tag="PKI23_01850"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNN35713.1"
                     /translation="MASRLLRIATRKSPLALWQANDVRARLLALHPGLRVELVTFTTR
                     GDKLLDAPLAKIGGKGLFVKELEQAIFDGEADLAVHSMKDVPMELPAGLTIATLCARE
                     EPADALVTPLPCTLLDLPQGARIGTSSLRRQAQLRALRPDFELLTLRGNVNTRLARLD
                     EGQFDAIILAAAGLRRLGFAARIRESLDDRLLLPAAGQGAVGIECRQDDVALHELLAP
                     LADLPTTLCVTAERAMNRRLDGGCQVPIAGHAQLSPRGLWLRGLVGDVERGTLLRAEQ
                     WGDPAQAEAIGIAVAEQLLRAGAGPLIEAARVDAAAD"
     gene            9321..10121
                     /locus_tag="PKI23_01855"
     CDS             9321..10121
                     /locus_tag="PKI23_01855"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNN35714.1"
                     /translation="MQPQTRPLAGLDLWVTRPAGQADRLIALLQQAGAAVSHLPLLAI
                     EPLPPTAAARSLLLDLDRYQAVIFISSNAARLGAEAILDLWPQLPTGIDWLAVGAATQ
                     ATLAEFDITAEIPAGGSDSEALLALPQLGSERVAGRAILIVRGTGGRETLREQLQTRG
                     ARVDYLELYRRICPASTLEQIRNWSRPLPDLVIASSGEGIDHLRRALTGPLLAMLERP
                     LLLPSTRLAQLAGELGFRQPLVADDASDDALLTCLLQWRARNAETLLA"
     gene            10099..11226
                     /locus_tag="PKI23_01860"
     CDS             10099..11226
                     /locus_tag="PKI23_01860"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNN35715.1"
                     /translation="MPKPSLPDSPEPALLGAAQVVQASHTAPAARPVSRWPAWLALGI
                     ALLGLLALAALLLWLQPQLSTAWQRLDQLSDASSRLDGRIDGLLQQLEQQGTEQQEQA
                     ARLTAQQEQLGQRLDAVTAAQQRFNQGDTPRQWRLAEAEHLLRLANDQLLAGRPLRFA
                     RQQLATADRLVAELDDPQLQPLRAALAADLAALDGHAPADVEGQFFRLSAGIGQVDRL
                     PPRGTPSLAEATTGEPAAPVQPGWRGWLDRIWQQLRPLVVIRQRTQSVEPLLTSSQTA
                     MLRQQLQLQLQLAQLALLRGEPSIYHTSLDAALDLLDRQFDGNAAEVTALRATLRELT
                     QQPIATAAPTLEASLSAVRQAIEAERNTPAMTPSPFPAEAP"
     gene            11229..12482
                     /locus_tag="PKI23_01865"
     CDS             11229..12482
                     /locus_tag="PKI23_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNN35716.1"
                     /translation="MRRLPWMLLLALPLTVLLVLLAQRDPGYLLLSYGGFSIETSLWF
                     ALLSLLVLLWLLRLLQRLLRGGQRLGSWWQHRDVRRQAQRVDEGLIAFAEGQWRQAHH
                     RLSRLGGAHHERLPLRLLAAQAAERAGDLQASQALLASIRQEGSAEDRLAADLTEARL
                     WIDRGAPERAVALLDALGAAHPDHPARLRLLAEALLRQRAWPLLTELLPKLKKPKLLN
                     DDELRTLERTLQCGLFSAAVAQGAAALEQQFTRLSRPLARDPQVIAAYVDALIESGAE
                     PRAERAVREALGRDWQAALVERYGRIGGEHPDQQLATAERWLKSHPHDAILLRAVARL
                     ALRNHLWGRAQQALQASFALHPDPQTAAELARLDTALGEATTGIGHYQQALGLLAVTL
                     PELPLPGKPRPSSGRLPGQIDPHRT"
     gene            complement(12441..13349)
                     /locus_tag="PKI23_01870"
     CDS             complement(12441..13349)
                     /locus_tag="PKI23_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNN35717.1"
                     /translation="MLHTLCWQPDHRHPATDNETLLAAAQRHGFSLPASCRNGNCERC
                     RSQLLRGRVLTAQGIIEADGTSCAILPCCAIALSDLLLDNPALLPPSTLPQVQRACQV
                     AALDPTADGCWRLLLRTPAAGGLPRYFAGQRARLIYADRQVELHFRAPWPDGRLLEFL
                     LDPLPLGDLWPSLFDWLRREPVIRCHWPVGEWHAGRLHEADSLLIACDRHGFAGAASL
                     AEWRLQRQVSPCTGWLWLDDATCAPPPHVEQWQQAQPHLRQVDWDGCMGFARVLIFCA
                     ADTGQSWRARLCRSGAMRIDLAGESA"
     gene            complement(13365..14831)
                     /gene="ubiD"
                     /locus_tag="PKI23_01875"
     CDS             complement(13365..14831)
                     /gene="ubiD"
                     /locus_tag="PKI23_01875"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HNN35718.1"
                     /translation="MRYRDLRDFIALLEQRGELKRIRTPVDPRLEVTEISQRTLLRGG
                     PALLFEQPLNHSMPLLSNLFGTVQRVALGMGEEEPSRLRAIGETLAALREPEPPRGLK
                     DAWEKWPLLKQALNLAPKLTRTAPCQSQIFEGSEVDLGRLPIQTCWPGDAAPLITWPL
                     VVTRGPEKPRQNLGIYRQQVIDRNRVIMRWLAQRGGAQDLREFWQRHPDRPFPVAVAL
                     GADPATILAAVTPIPDTLSEYAFAGLLRGERSELVNCIGSDLQVPASAEIVLEGVIHP
                     GDFADEGPFGDHTGYYNEVERFPVLTIERITQRERPIYHSTYTGKPPDEPAILGVALN
                     EVVVPLLRKQFPEIIDFYLPPEGCSYRLAVVTIKKGYPGHARRIMLGVWSFLRQFIYT
                     KFVIVTDDDIDARDWKEVIWAVTTRMDPVRDTLLIDQTPIDYLDFASPVSGLGGKMGL
                     DATHKWPGETQREWGRPIRMDAAVKARIDQLWPELGID"
     gene            complement(14845..16104)
                     /gene="rho"
                     /locus_tag="PKI23_01880"
     CDS             complement(14845..16104)
                     /gene="rho"
                     /locus_tag="PKI23_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNN35719.1"
                     /translation="MNLTELKTKPIPELLQIANEMGLENMARSRKQDIIFAILKKHAR
                     SGEDISGDGVLEILQDGFGFLRAAEGSYLAGPDDIYVSPSQIRRFNLRTGDTVVGKIR
                     PPKEGERYFALLKVDTINYDTPDSSKNKILFENLTPLFPQRRLLLEAGNGSTEDISAR
                     IIDLVSPIGKGQRALIVSPPKAGKTILLQNIAQSIARNNPECYLIVLLIDERPEEVTE
                     MQRMVRGEVIASTFDEPPTRHVQVAEMVIEKAKRLVEHRMDVVILLDSMTRLARAYNT
                     VVPSSGKVLTGGVDANALAQPKRFFGAARNIEEGGSLTIIASALIDTGSKMDEVIYEE
                     FKGTGNAEIHLDRRAAEKRIYPAINIRRSGTRREEFLTSEDELQRMWILRKLLNSMDD
                     LDAIEFLVDKLRQTKSNDEFFASMKRK"
     gene            16684..18687
                     /locus_tag="PKI23_01885"
     CDS             16684..18687
                     /locus_tag="PKI23_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNN35720.1"
                     /translation="MHASERGPENRDSRLMVLSLAALGVVFGDIGTSPLYALRECFFG
                     STQLPVNEANVLGVLSLIFWTLICVISIKYQTVVLKADNRGEGGILALLALLDPWHSS
                     QGGNKPKTLVILAIIGASLLYGEGVITPAISVLSAVEGLEVAAPGLAHYVIPITVVIL
                     IGVFLLQSGGTHKVGALFGPVMVVWFSVLTILGLLAIGRNPAVLAALNPLHAVNFFID
                     NKMVGFFTLGAVFLVVTGGEALYVDLGHFGRKPIRRVWFFLVLPALVINYLGQGALLL
                     TSGGAIKDPFFALAPEWGLYPLIILATMATVIASQAVISGVFSLTNQAIQLGQAPRMN
                     VVQTSPNEIGQIYIPFLNWVMMLTTIALVLGFKSSSNLISAYGISISTAMLITSLLTF
                     FVMSEKWQWPRPAALAIAGLFALIDLSFFSANVVKIEHGGWFSVLVALLVYTLMSTWS
                     SGRQAVINQLRGSVAPLDEFLSHFTAQWPLRVPGTAIFMTAPDLGVPPILQYHLMHNQ
                     VLHERVILLTVVTEDEPWVPASKRLKIVELSHGFYRVHVTYGFMQSPNLPVALRLGER
                     LGLTFDPEATSYYLGRETLTPHGSVSRMMRWRQMLFAVMARNAVRATDFFKLPPYRTV
                     ELGMRLELMPMAYLGLKPHKKNGNGKQSEPDAAAAPADSSIVP"
     gene            18887..20077
                     /locus_tag="PKI23_01890"
     CDS             18887..20077
                     /locus_tag="PKI23_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNN35721.1"
                     /translation="MAYDSTYMRGKAAIVGAVDAVSPTGELGIHERTLQVQMIHAALE
                     DAGLRLSDVDALFATGTMMSSMELAEHLMITPRYTDTTMTGGSSFEVHVEHAAAAIAL
                     GLCEVAVIVYAATPRAKGGANFMAAQMAALDKNNPPPNIEWELPYGTISPPGNYALAA
                     SRHMALYGTTRAQLAQIAVSTREWACKNPRARHQEPLTLKEVLEAPMIASPLGKLDCC
                     LVTDGAGALVMTSAERARSLRKKPAYVLGAGTAHDHAMMISQMPDLCTTAGAISGPKA
                     FKMAGITPKDVDVVEIYDSFTITVALALEDLGFCKKGEAGAFVQDGRLGPGGALPTNT
                     NGGGLSYTHPGMYGMFLLVEAVKQIRGESTGQVKDAKIAVAHGCGGILSSTSTVVLGS
                     EETL"
     gene            20074..20472
                     /locus_tag="PKI23_01895"
     CDS             20074..20472
                     /locus_tag="PKI23_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNN35722.1"
                     /translation="MKRMILGTSPVAAPFWEATKEKKFLLQFCATTGKAIWYPRDFSP
                     HALGGNVEWREASGKGVVYAVSVMHRPGNPAMAEAVPYAVALIDLEEGARMLSNVVNC
                     DPDSIKVGDKVKLAWEPLEGGRHLPVFELA"
     gene            complement(20684..21784)
                     /locus_tag="PKI23_01900"
     CDS             complement(20684..21784)
                     /locus_tag="PKI23_01900"
                     /inference="COORDINATES: protein motif:HMM:NF023015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNN35723.1"
                     /translation="MPNVKIVEKMHRDLIDLHQQEEINARLGPNEIEIIREIFHTPLV
                     GSYNWDYSITNDRLERLYELGKQLNWNAEDDLDWDTPVDRTKFITDQKYNAFRGFAPY
                     EAMSEEKKIEFDWHMMAQKCSNFLHGEQGAMLVASQLVSCAPTYEAKLYASSQTFDEA
                     RHVEVFNKYLRRKIGFMYPISETLKAILDKVLGDERWYLKFIGMQLIIEGLALAAFNS
                     AKMSAVDPLFAQVCHLVIRDEARHVTFGVNFLEDFVKTLPTQEREDAAAIAFDVCVLM
                     RERLITSDVFAQFGWDPEEARKWQLDSDVAGNFRNLLFTRVMPNLNRVGLLTDKIRSK
                     YDQLGLLTYSTYPDNHEIDWVELEKPLFDKSA"
     gene            complement(21904..22650)
                     /locus_tag="PKI23_01905"
     CDS             complement(21904..22650)
                     /locus_tag="PKI23_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HNN35724.1"
                     /translation="MIRRLSWVLLLCGSMLGIQAVLAESTAEATIRAAIEKGVPRVRI
                     AEISPSPITGIYRVTTEQGPVLYSTADGSHFIVGDLYRLTGAGAINVSEEGRAQERVK
                     LLSEIKPEEMVIFPGQPSDHAITIFSDVDCGACRELHKMVPALNKAGIEVRYLAFPRE
                     GLSSATHDKMVSVWCAKERQQAFTDAKAGKKVPTARCSNPVAKEYALGLKLGVQGTPA
                     IILADGQLIPGMVPFENLVAAVKGEPGAAE"
     gene            complement(22688..23410)
                     /locus_tag="PKI23_01910"
     CDS             complement(22688..23410)
                     /locus_tag="PKI23_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HNN35725.1"
                     /translation="MRAVYQSLDRLQFALLPGHCLLCAMGSNRPLDLCAPCEAELPWI
                     THGCRRCALPLPAAELLCGRCQAQEPPFSQVVAPLAFAPPLDQLLRDWKQQPQPALGR
                     LFADLLASHLRVGRLASAPPPELLLPVPSHPRRLRQRGFNQAQELAVGLGRRLGIEVD
                     CHLLKQRGSGVDQRQLDFKARQRNLRDAFVVKGLLAGRRVAVVDDVVTTGATAQAMSR
                     VLRRAGAIEVEVWAVARTPRRG"
     gene            23531..24514
                     /gene="bioB"
                     /locus_tag="PKI23_01915"
                     /pseudo
     CDS             23531..24514
                     /gene="bioB"
                     /locus_tag="PKI23_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
     gene            24612..25814
                     /gene="bioF"
                     /locus_tag="PKI23_01920"
     CDS             24612..25814
                     /gene="bioF"
                     /locus_tag="PKI23_01920"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010444223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNN35726.1"
                     /translation="MNPLLEALTQRLETRVAQQLYRQRRVTDSPQQPLLKQDGRELLA
                     FSSNDYLGLANHPRVIEAFTAAVRQYGVGSGASHLINGHLRPHHQLEEALADFTGRER
                     AVLFSTGYQANLGTLSGLLGRHDRIYQDRLNHASLIDGATLSRARLLRYRHADSGHLA
                     ELLSRRPPATTALIATDGVFSMDGDLAPLPQLARLASDHGAWLMVDDAHGIGVLGEQG
                     GGSLERFGLGQAQVPILMGTLGKALGTFGAFVAGAAPLIETLINTARPYIYTTALPPA
                     VAAASCVSLELLRQEGWRRERLRQLIDQLRAGVASLGLPLMPSTTPIQPLLLGSSSTA
                     LAWSQALEAQGILITAIRPPTVPQGSARLRITLSAAHQPQQIDRLLEALDKVRRQLGA
                     PELAQANP"
     gene            25811..26635
                     /gene="bioC"
                     /locus_tag="PKI23_01925"
     CDS             25811..26635
                     /gene="bioC"
                     /locus_tag="PKI23_01925"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HNN35727.1"
                     /translation="MNPPPSAETVAAAAAPPIDKGRIARSFGRAAGSYDSVADLQRHA
                     IQRLIEQLPADLTPQRIVDGGSGTGLGALALRERYPQAQLTLLDLAPAMVRHARARPP
                     LQAAQHLCADLEAVPLASGSVELLFSSLALQWLPRLDLFLDEAWRLLAPGGWLLFSTL
                     GPATLQELRGAWASVDANVHVNRFLPVDQIETALGRHPWRQRRLIPAPLVRHYPSLRA
                     LTHELKALGAHNLNPDQPTALTGRQRITRVSAHYETLRTATGLPASWQLLLGVLQR"
     gene            26632..27306
                     /gene="bioD"
                     /locus_tag="PKI23_01930"
     CDS             26632..27306
                     /gene="bioD"
                     /locus_tag="PKI23_01930"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNN35728.1"
                     /translation="MKRAYFVTGTDTDAGKTLVSSALLLRARQQGWQTAAVKPVATGG
                     LPHQGVLKNDDALQLQRQSTLPLDYALVNPVCLHDPIAPHIAAQEAGIALHAALLATA
                     CRRVLALGADFTLIEGAGGWRVPLNDRETLADLARLLGLPVILVVNMKLGCINHALLS
                     CEAIAADGLPLAGWVANRAAPQMSRFEQNLASLDRLLPAPRLGMVPHLTPVSAEQAAP
                     WLRLPD"
     gene            complement(27316..27762)
                     /locus_tag="PKI23_01935"
     CDS             complement(27316..27762)
                     /locus_tag="PKI23_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNN35729.1"
                     /translation="MRPRHATRLALLCLPLLGGCSAPPPQNIGVVDNHLHPCPQSPNC
                     VSSEAAAADAEHAIAPLSYSGSTEQARARLEQLLGSQPRVRLVTVSPTYLHAEFTTRL
                     LRFVDDVEFLFITPGRIEVRSASRVGRSDLGTNRERIEMLRRQFSG"
     gene            27900..29198
                     /locus_tag="PKI23_01940"
     CDS             27900..29198
                     /locus_tag="PKI23_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNN35730.1"
                     /translation="MELPHFNDGLFRYLRRSPTPFHAVAEAVRLLVERGFTPLNEADG
                     WSLTPGQRYLVTRNGSSLIAFVLGQRSPLESGIRMVGAHTDSPCLRVKPEPVLRSQGC
                     LQLGVELYGGVLLNPWFDRDLSLAGRATCLTTRGELVNLLIDLKKPLAVIPSLAIHLD
                     RDANSQRSINAQNDLPPILLQLDDRQSFSFTLWLREQLQLAHPEVEIGEVLGHELSFY
                     DTQPPAYVGWDDQFIASARLDNLLSCHVALAAIAEAGSELSTLIVLNDHEEVGSGSAA
                     GAQGPFLRAVLERMVPGSEALTRAIDRSLFVSADNAHGIHPNHAAKHDANHGPRLNQG
                     PVIKFNASQRYASNSETAAWFRHLCTQSRVPVQSFAVRSDMGCGSTIGPLTAAEIGVR
                     TVDVGVPTFAMHSIRELAGRSDAHHLYRVIKRLFATERLS"
     gene            complement(29204..29515)
                     /locus_tag="PKI23_01945"
     CDS             complement(29204..29515)
                     /locus_tag="PKI23_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HNN35731.1"
                     /translation="MAVETDAEGYLCRLDAWSEAVAQQIALAEGITLTAEHWQIIHLL
                     REFYRRYQQSPAMRPLVNFIARELGPEQGRSIHLLRLFPGSPAKLAAKIAGLPKPTHC
                     L"
     gene            29787..31802
                     /locus_tag="PKI23_01950"
     CDS             29787..31802
                     /locus_tag="PKI23_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNN35732.1"
                     /translation="MKTKPPHPFTPRLLSLMVAVAFLPSVPAMAANCVWNPAAGNWAT
                     AGNWSCAQVPGSADSAGIAGGQTVMVNSAQSIFNLNNAGSLNIDIFLLTLNGGGATRN
                     SGAINVGSGAIPFNAALQVGGGHNIDNSGGTINISADSVLNQFGSTISGGSINTFGSG
                     KVVAFNSNSNALSAVTLNGNLDLASATGVERVFGGLTLNGVIDLNSNSVLSFGGDQTL
                     GGTGSVVLGDSGSSNRALSLEGDTTLTIGSDMVVRGKHGTLGQAYLSGGSQKLVNDGR
                     IAADVSGGILYLAPSHGTTNNGVIEAQNGGTLVLNSNVAGTTGSALNAGAGSLILQNG
                     VTLSGVINTSGSGNLRASNSNSNALSAVTLNGNLDLASATGVERVFGGLTLNGVIDLN
                     SNSVLSFGGDQTLGGTGSVVLGDSGSSNRALSLEGDTTLTIGSDMVVRGKHGTLGQAY
                     LSGGSQKLVNDGRIAADVSGGILYLAPSHGTTNNGVISTVAGSTVSIGNSFLNAETGL
                     ITGNGTVVAPSGGLTNAGHVAPGASPGTLTIAGSYVQTTAGTLDIELADLSSFDLLKV
                     TGNATLAGTLALHCFGACNFAVDDTLKILDAADNASSLTGSFDSLLLSGFGSGQFDVI
                     YDRALGDVLLKVTQAVTPAPVPLPAASWLMLGGLGLLGGLRRVKRAG"
     gene            31975..32748
                     /gene="ubiE"
                     /locus_tag="PKI23_01955"
     CDS             31975..32748
                     /gene="ubiE"
                     /locus_tag="PKI23_01955"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNN35733.1"
                     /translation="MSHLDPAADEGSQTTDFGYQQVPLRQKRARVAEVFDSVAGRYDL
                     MNDLMSFGIHRLWKRLTVEVSALRPGDWALDVAAGTGDLTDLMSRKVGRHGRVVMSDI
                     NSAMLQRGRDRLLDRGVAGNVDWLISDAEALPFADDSFAVVTIGFGLRNVTDKARALA
                     EMARILKPGGRLLVLEFSKPTHRLLEQCYDRYSFSVLPRLGRLVTQDSESYRYLAESI
                     RMHPDQERLRAMMAEAGLAECRYHNLTGGIVALHRAIKP"
     gene            32759..33373
                     /locus_tag="PKI23_01960"
     CDS             32759..33373
                     /locus_tag="PKI23_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNN35734.1"
                     /translation="MTVLRRLALLPLEQSINQLLHHDPRSLARLEALAGHCIAVRLTT
                     PPLRLFLLPQHGGVSLRLDHPTTADVQIVATLPALVAYLGGHDSGPALQLQGDAELAG
                     TLQSILAQLDIDWEAALSRLTGGLLAHQTGRLLRATRRQLARGGDHLTDMVANWLVEE
                     SHTLPAAVEVARFNDAVDQLRFDSDRLEARLKRLERQRGGHGSA"
     gene            33370..34995
                     /gene="ubiB"
                     /locus_tag="PKI23_01965"
     CDS             33370..34995
                     /gene="ubiB"
                     /locus_tag="PKI23_01965"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNN35735.1"
                     /translation="MKNLLRLLSILRVLLRHRLDLLLPASHRPAPLRLLLWPLALLPA
                     PVRPPGERLRLACESLGPLFIKFGQMLSTRRDLLPEEIADELKKLQDQVPPFASSEAM
                     ALVERDLGQPIEALFSSFDPTPLASASIAQVHAAQLPDGRAVCVKIVRPTIEPVIRRD
                     LALLQRLADFAERNFNDARRLQLSRVVRDYQTTLLDELDLKREAANTMQLRSNFAHSP
                     LLYVPEVHWDYSRPSLLVMERIHGIAVSEIDQLRGAGIDLKKLAERGVEIFFTQVFRH
                     NFFHADMHPGNIFVSTRRPDSPQYIAIDCAIMGALTEADQRYLATILHAFFQRDYQEV
                     ARLHSESGWIGAAVPVHDFAAAIRTVCEPIFQKPLKEISLGELLIYLFQTARRFDMEI
                     QPQLVLLQKTLLQIEGLGRQLYPDLDLWSTASPFIQRWMQERSGPRALAGRLLRQLPN
                     WAEQLPELPRRLQQSWLQIDQLGQLQRAQAAALQQLQQGIERRQRRQRLIALLLLVSG
                     LGWLFSSHHDPQGSLSGGGLLSLLAGLWLYAAK"
     gene            35024..35365
                     /locus_tag="PKI23_01970"
     CDS             35024..35365
                     /locus_tag="PKI23_01970"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNN35736.1"
                     /translation="MSDVLQRLAEVLEARKTMAAADSYVASLHAKGLDQILKKVGEEA
                     VETVLAAKQMQSSGDGEPLIRETADLWFHCLVMLSHLGLHHDQVLAELQRRFGLSGLA
                     EKAARPTSNHS"
     gene            35377..35601
                     /locus_tag="PKI23_01975"
     CDS             35377..35601
                     /locus_tag="PKI23_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNN35737.1"
                     /translation="MGRFGLTELLLIFGIVLLLFGTKRLRNLGGDLGNALKGFRDAMK
                     DDKGGDKVEEQPSARVIEGEAKEKSPERQP"
     gene            35617..35922
                     /gene="tatB"
                     /locus_tag="PKI23_01980"
     CDS             35617..35922
                     /gene="tatB"
                     /locus_tag="PKI23_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNN35738.1"
                     /translation="MFDIGFSELLLVGVVALLVFGPERLPELARNGALWLGRLRRAMR
                     DAKQSIKDELGTDEIMQQLHNEEVLRKLGEDPNRLSRPAPPPAPPVTTAHDPANERH"
     gene            35909..36694
                     /gene="tatC"
                     /locus_tag="PKI23_01985"
     CDS             35909..36694
                     /gene="tatC"
                     /locus_tag="PKI23_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNN35739.1"
                     /translation="MSATDPQADDAATESSLLFHLRELRSRLIRIVAVTVLLLLALLP
                     FANEIYLFFSRPLTALLPAGSSMIATEVASPFFAPFKLTSVVALLLAMPYLLYQLWSF
                     IAPGMYRHEKRFALPLLLASILLFYGGIAFGYFLVLPTLFAFFTSTAPQGVALMTDIN
                     QYLDFVLKFLFSFGITFEVPVATVLLIRAGIVSAAALADKRPHVIVLCFVIGMVLTPP
                     DVFSQTMLAVPMWLLFEAGLLCGRLLTPPAEPVKTEVTTQPPH"
     gene            complement(36721..37158)
                     /gene="dtd"
                     /locus_tag="PKI23_01990"
     CDS             complement(36721..37158)
                     /gene="dtd"
                     /locus_tag="PKI23_01990"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNN35740.1"
                     /translation="MKAVIQRVAWARVVVAGEEVGAIGAGILALIGVEPDDTPARAAL
                     LLDRLVRYRIFADESGRMNRSLLDTGGELLLVPQFTLVADTRRGLRPSFSSAASPAQG
                     RELFDLLLAQAAQLPCKVASGRYGADMAVSLLNQGPVTFWLES"
     gene            complement(37155..38126)
                     /gene="pip"
                     /locus_tag="PKI23_01995"
     CDS             complement(37155..38126)
                     /gene="pip"
                     /locus_tag="PKI23_01995"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNN35741.1"
                     /translation="MQVLYPEIKPYQRHWLKVSDLHELYLEESGNPRGIPVLFVHGGP
                     GAACDANSRRFFNPEKYRIICFDQRGCGRSTPHAELKENTTHHLVGDMERIRTLLGIE
                     RWLLFGGSWGSTLSLVYAQSHPERVLAMVLRGIFLCRQQDLHWFYQQGASLVFPDYWR
                     DYLEPIPPAERDAMITAYYHRLTGSDEIARMGAAKVWAQWEARCSTLKPRKDLIDWFI
                     KPQRALALARIEAHYFAHETFLEANQILRNAERLAGIPGMLIHGRYDMVCPLDNAIAL
                     QNHWPDAELQIIREAGHAATEPGITDALIRATDEMARRFSDGTLPTA"
     gene            38437..>38924
                     /locus_tag="PKI23_02000"
     CDS             38437..>38924
                     /locus_tag="PKI23_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35742.1"
                     /translation="MLHPLLAEFISDRLERRFLRTLWPHQRDQIYLLSLIGGLLFSIA
                     VYGDYLRFGAATLSTPIGLARFGLILLCALPAVAAACDRARLANQLCFLSMAGIGGLY
                     LYLCHAQALDPQARLGTLFLMTTLFYLCAPLRLLWTITFNLLLSLGFLWVNSLTPGIA
                     PY"
BASE COUNT         6515 a        13123 c        12669 g         6617 t
ORIGIN      
        1 cggcgagcgt cggcggcaaa ccgtggaagt atctgctggt gccgcacgac gaggtcaatg
       61 agtcgcggcg gcttgcggat tacctgcact ttgaggtcaa gccgcactcc tgcggttgag
      121 cgcgccatga tggcgcttgc agcccgctga caccgaaaaa ccaccgccga agaggctcct
      181 gggcaaagaa cagatggcga ccgaaacccc tgcgcaatcc tcgctcgccg ccctgctgcg
      241 gcagcgcgcg ttcatcttct tctggctggc gcgggtctgc accgccagcg cttttcagat
      301 gcagggcgtg gtgctgggct ggcaggtcta tcagttgacc ggccgtgcgc tcgatctggg
      361 actggtgggg ctctgtcagt tcctgccccg ggtggtgctc accccttggg cgggccatgt
      421 cgccgaccgc ttcgaccgcc gccgcgtcac cctgctgatg caactgctgc agggaatact
      481 gctgttgctg ctctgcctgg tcagtgcgca gggtgcggtg aaccgcgaac tggtgctgct
      541 gctggtggct ctgggcggtg ccgcccgcac cttcgaggtg ccggccaccc aggcattgct
      601 gtcggcagtg gtgccaccgc tgctgctgcc acgcgcggtg gcgatcagct cttcggcaat
      661 gcaggccgcc acactgatcg gtccggtggt ggccgggctg ctctatctgc tcggcgcgac
      721 cgaagcctat ggcgtcaccg cactgctcta tgtcgccgcg gcggcgatga tggcggccgt
      781 gcggccgttg gttcatcagg accgcagcgg cccgcagagc aactttctgg cgtcgttcat
      841 ggaggggatt cgctttttgc gccgtcaacc gatgctgctg ggcgccatct cgctcgacat
      901 gttcgcggtg ctgctgggcg gcgccaccgc gctgctgccg atcattgccg atcagttgct
      961 gcagaccggt ccggtgggtc tggggctgct gcgcgcggcg ccggcggtcg gcgcgctggc
     1021 gatgtcgttc tggctggcca gccacccgct gcatgcggcc ggtcgggcgc tcttcatcgg
     1081 tgtcgcgctg ttcggcctcg ccaccctcgc gctcgggctg tcacgctcgc tgccgctgtc
     1141 gctggccatc ttgctgctgc tgggggcggc ggacatggtg agcgtggtga tccgtcagac
     1201 cctggtgcaa ctgcacacgc cggatgagat gcgcggccgg gtcagttcgg tcaacgccat
     1261 cttcatcggt gcgtcgaacc agttgggcga gttcgagtcg ggcctcaccg cacagtggtt
     1321 cgggctggtg ccgtcgattc tgctcggcgg cagcggcacc ctgctggtgg tggggctgtg
     1381 gatgcgctgg ttcccgcagt tgttgcaggt caggagtctg gaagaggcga tgccggcgca
     1441 aaaccgaccc tgactggaaa ccggccaggc cctgcgcacg ccggcgccgc cacgcgagaa
     1501 aaggtcacgc ctccagcagg ctgcgcagca tccaggcggt cttttcgtgg agattcagcc
     1561 gctgcgtcag cagatcgatc gtcggctggt cgttggcggc atcggcgatc ggcagcagct
     1621 tgcgtgccgt ccgcgcagtg gcctcctgcg cggccaccag atggcgaacc atctccatcg
     1681 cggcgggaac tccttcgacc tcctcgatcg aggccagctt ggcaaactcc cggtaggtgc
     1741 cgggcgccgg atgccccagg gcgcggatgc gctcggcgat cagatccagc gcattccact
     1801 gctctgtgta ctgcaccatg aacatctgat gcaggctgtt gaagtgcggt ccggtgacgt
     1861 tccagtggaa gttgtgggtc atcaggtaga gggtgtaact gtccgccagc agggccgaca
     1921 gcccatcgac gatcttctgg cgatcagcgc cgtcgatgcc gatgtcgatg gctgaaccct
     1981 tggacttctt ttctttcatg cgttctgctc ctgtcgtgcc gagtggggat cgaaccggcg
     2041 cggacaacgc agcgagcccg ccggatcacg atcaactcta atggctcggg caacagaaaa
     2101 gaagctggta tcagaggccg cagcgcacgc gccagcaatc agcccaccga ctcctgccgc
     2161 atgaagaaac ggttgatcgc cgcctcgatg cgcaccttgt ggtgcaggta gctggccgtg
     2221 gagggtggcg gaccaccgag cgcacccaga tcgaggtcgg agagatgggc cgggtagcgt
     2281 tcgccgctgc gacgttgccg aagcagcgcc tcggcgacgc gaccgtagag cgcttcgcca
     2341 tcgtgccggc tggaaaattc ggcatcgcca acccgcagca tgaagcggga ttcgccgccc
     2401 tccgccggct cggccaccgc ctcgacaccc tggtaggcga gccgggagac cagactcgca
     2461 aagctgcgcg gcgtggcgag tggttcgcgt ccggtcagct cgaagcagtg cagcgccagc
     2521 gcgggcgcgc cgtcgcgctg gcgtcgctcg tcgatccgct ccagaaaacc gagccaggga
     2581 cccaccgcct gccgcggatc gctgccgggg tgcgcaagcg cgaagagcgc gccgctctca
     2641 tcgacgatgt aaatgcggat ctgttgcccc tgcagccggt agagcagctc gatccggttg
     2701 ggctgattga gccgtgcgac gacatgcagg tcatgttgcg gcaggctcat cgggtcgatc
     2761 tgcaaggtgc ggaactgtgc cgccggcgtg gccagatggc cgaacagggc gcccatcgag
     2821 ccgaatcggc gctgcacgac acgtgcaccc tcccactcca gcatgaacag ctccttttcg
     2881 atctcgaaca ggtagcgcgc cgtccggctg ccatgcggtg caaaatgctg ccgcaccgcg
     2941 acaaagcact gctcgatgcg ctcgctgatc agcatctccc gcccctcggt catgcaactg
     3001 atgaccggcg gtggcggcag gcgctccagc gccatgccat ggccgttgag gtggtcgacc
     3061 agggtgtcga tcagggcggc attgccgaca tggcgctggg tcagcaactc gccccagctg
     3121 gtgaggatga aactgtcgat ggtgcgcacc agattctgcc gcagaccact gtaaccgagg
     3181 gcatcgctgc ggctggagat gcgggccaac ccggaggcat gcagtgtgga gggggtctca
     3241 ctgtcgaggt tgatcaccaa catgctggag agtggctgct gcacgctggc gaaggcctga
     3301 tggggggtgg gcggcaacgg catcggctgc caacgctcca gttcggcaaa cagacgctgc
     3361 agggcccgtt cactgaggct gccctccact gtgctgcggc tcgaccgtcc ggccaactgg
     3421 ttgaagtgag cccaggcgat caactcgccc aactggcgcg cctgccggat cggcggactg
     3481 gtcgccacat cgacctggtc gaacagtgcc caaccggcgt cagacgccgg tcgcggtttg
     3541 aagtgcagcc gcggggcgaa gggcagcggc agacaggcag gttgcaggtc gatcttgccg
     3601 gggcgacgct cgaaggcggt gttgagtttg cgctgcagga cctgcaactc cctggcatcc
     3661 atgacattgg tcaactgccg gtcacgggca aaacgggtga gcgcgcgata actgaaggtg
     3721 agttcgacca ccagccgccg ccactcctcg atggtgcgct cgatgctcca ctgcgcgcga
     3781 tcgaggcgag cgaggcgccc acggtcccag ccccattcat cggtcaaccg ccgcatcagc
     3841 gcccgccgcc agccagtgcc accgccctcg gccttgtggt agaggcagcg tcgaatcagc
     3901 tcgatccgct gcggatcatg ctgctcctgc agatgggctt cgagccggcg gtagagaaag
     3961 acatagggat cgagctggtc cgggtcggtg ataccggcgt agatcgccgt cttgtagtcg
     4021 agcgccagcg cacggttgac cgagtgctgg ctggcgtagc attcgatcag cagcagcttg
     4081 agcaccgact tgtagggcgc atcgatcgac ttgaacagtt gccagatgcc cgcgcccaga
     4141 aactcctcgg acggcacccg cgccaccgac ccgaagtcga tcacctcccg ggcgtcgatg
     4201 aagcggcgct gccgcagcca ttcggcatgt tcggcatggt ggagctcgag ctcgggcggc
     4261 gtcagccacc agatcgggta gcgcccggcc agcagcagcc cggtgcggta gaactcatcg
     4321 agcagcaggc catgctgggc cgagccgctg ctctcgctcg acagctcgct ctgctcgccg
     4381 cgacgaaagc tgtcgcaatc gaagacgaaa aagtgcaggt cgagatcgag ctccgccgcc
     4441 cagcgtgcga tgcgctgcgc cttgcgtcgc aaccgatcga ctgccgcgtc gtcgagaccc
     4501 gggcggtggc agagccagat gtcgaggtca ctgctggcat tgcaggcaaa ggagccgaca
     4561 ctgcccatca gataaatcgc atcgatgtcg cgctgctgct cggagaccgg gttgtagcga
     4621 aaggcgcggc tcaactgctg cgcggcatgc agcacctcgc gccccggctg atagccggcc
     4681 acaccgaacg gggttggccc gctgccggcg ccgggcagca gtggatggtc gaggtgaaac
     4741 agcagcggca gcagatcgag cagttggcgc tgccgttcgt tgcgcgatcc gcggtagcgc
     4801 tccagccggg catcgttgac ccggcggaaa cgttcgacgg tctggcgcaa cgccttgcgg
     4861 tcgacgccgg cgtcgctcca agtctcgact gtgctgctgt ccatcccata actatagaca
     4921 gccgaacgga atccggcccg tcgcgggcca cccccggcta gcgggcggca gcgcgcccgg
     4981 ccagcccggt cctggcacgg gccacagcct cgcgcacctg ccgtggcgcg gtgccaccca
     5041 gatggtcgcg ggccgccacc gacccttcgg gggtcagcac ctggtagaca tcctcgtcga
     5101 tgctggcgca gaactgccgc agcaactcga gcggcagctc gctcagcgcc agttgctggc
     5161 cgatggcgtg ctgtactgcc cgaccgacga tgtggtgggc atcgcggaac ggaacgccgc
     5221 gcttgaccag gtagtcggcc aggtcggtgg cggtggcata gcccgactcg gccgccgcgc
     5281 gcagcaccgg tcggttgagt tcgatcgccg ccatcatgtc ggtgtaggcc agcagcgagt
     5341 cggtcacggt gtcgatggtg tcgaacagcg gctccttgtc ctcctggttg tccttgttgt
     5401 aggccagcgg ctgccccttc atcaacgtca gcagcgcgac cagatggccg ttgacccggc
     5461 cgctcttgcc gcgcaccagt tcgggcacgt cggggttctt tttctgcggc atgatcgaag
     5521 agccggtgca gaagcggtcg ggcagctcga tgaagcggaa ctgggcgctg ctccagagga
     5581 tcagctcttc ggagaagcgc gagaggtggg tcatcagcag cgcgcaggcg ctgacgaact
     5641 cgatggcgaa gtcgcggtcg ctgaccgcat ccagcgagtt gccggccggg gcgctgaaac
     5701 cgagcagcgc ggcggtcagc gtgcggtcga tcgggtaggt ggtgccagcc agcgccgcgg
     5761 cgccgagcgg catccggttg acccgcacgc ggcagtcgag cagccgttcg cggtcgcgtt
     5821 cgagcatctc gtaccaggcc agcatgtggt gaccgaaggt gaccggctgc gcgctctgca
     5881 ggtgggtgaa gccgggcatg atggtgtcgg cctcgcgctc ggccaggtcg accagaacag
     5941 cctgaaaccg gttgatgagc cgttcgatct ggtcgatctc gtcacgcagg tagagccgga
     6001 tgtcggtcgc cacctggtcg ttgcgcgagc ggccggtgtg cagcttcttg ccggccgcgc
     6061 caatcagctg ggtgagccgc tcctcgatgt tcatgtgtac atcttcgagc tcgatcgacc
     6121 agcggaactg tccgctttcg atctcctgac ggatctggcc cagcccgctg ctgatctgcc
     6181 ggaactcttc gttgtcgagc acgccgaccc gtgtcagcat ctcggcatgg gccagcgagc
     6241 cgcgaatgtc atgcagcgcc aggcgctggt cgaaaccgac cgaggcggtg aagcgctgga
     6301 cgaaggcgtc ggtcgcctgc tgaaagcggc caccccacag ttgatgggtc gaagaggcgt
     6361 cattcatcgg ttctggacta cgctataaaa gatggacggg gcggccattg taccaaggtg
     6421 atccgccacc gaacgaacct tgcaggagcc cgatcgattg caccgcacca ccgccaagcg
     6481 ccccgccacg gcgcaccccc tgccgacatc ggtcgaatcg ggactggtgc ccgacttcag
     6541 ccgggtcggg ccgctgctct ttctgctgct gctgtgcgag ctgctggtga tcgtgctggt
     6601 gctggccgcc agcggcgtgc gccagttcga ctggcagatg ctggcgctct ggtcgctgca
     6661 ggtggagtgg atcgcactca cttcggcact gctgatcagc cggctacgcg cccggctgct
     6721 gcggctggaa ccggccgccg cggtgagcgc actctatctg ctggtgctgg cggtggtgat
     6781 ggtggtgacc ctctgcggtc agtggttcac cacatgggca gtggccgaga ccaccgactg
     6841 gttcgatcct tacgcgctgg ccaccgcact ggtgatcggt gccatcacca ccggcgtggc
     6901 gctgcgctac ctctatctga gcgatcggct gcgcgtcgaa cagcaggcag cgctgaccgc
     6961 ccggctcgat gcgctgcagg cgcgcattcg cccgcacttc ctgttcaaca gcctcaacag
     7021 catcgcggcg ctgatcagca tcgatccgct ggcggccgag gagctgctgg tcgatctatc
     7081 ggagctgttc cgtgccacgc tcaaggaggg cgagcggctg gtgccattga cgcgggagat
     7141 cgaactctgc cagcgctacc tcgccatcga gcagcgacgc ctcggcgaac ggctgcgggt
     7201 ggtctggcaa ctggcacccc accccgacag cgcccggctg ccgccgctga cgctgcaacc
     7261 gctgctcgaa aatgccatct accacggcgt gcagccactg ctcgatggcg gcgagattca
     7321 catccggctg tcgcgccgag gcgagcgcat cgagatcgag atcaccaatc cgctgcctgc
     7381 cgccagcgcg gcgagcgagg ggcaccgcat tggattgacc aacacccggg cgcggctgcg
     7441 aaactgcttc ggcgaccgcg ccgaggtgca gggctctgcc accgatggcg gctaccgcac
     7501 gctgctctct tttcctgacc agatctgacg aggcgaagga cgcatgagtg agcgtggcca
     7561 gcgaggcatc gatgcagtgc gccgggtgat ggtggtggat gacgagccgc tggcgcgaca
     7621 gcggctggca tcgctggtca acgacctgga gcactatcag gtggtggcga tggccggtga
     7681 cggccatgcc gcgctggagc tgctggccct tgaggcgccg gagatcctgc tgctcgacat
     7741 ccgcatgccg ggactcaacg gcctcgacct ggcggcacgg ctgcgcgacc tgccgcgccc
     7801 gccggcgatc ctgttctgca ccgcctacga ccactacgcc cgcgaagcct tcggcgtgcg
     7861 cgcggccggc tatctgttga agccggtgcg ccgtgccgac ctgctgcagg cgctcgagga
     7921 tgcctgccag ctcagccggt tgcagtgggc ggcgatcgag caggcgcgtg cccgacccgt
     7981 cgcgggtggt ggccgcagcc acctgtcgat ccagcggcgc catggcatcg acctgatcgc
     8041 gctcgacgag gtgcgcgcct tcatcgccga acagaagtac gtcaccgtca tccatgcggc
     8101 tggcgaggcg ctgatcgatg agccgctgcg cgcgctggag caggagtttg ccgaccggct
     8161 ggtgcgcatc catcgcaacg cgctggtggc gctcgatgcg gtggaatcgc tcgaagcgac
     8221 cgacagcggc catcggctgc ggctgcgtgg cgtcgagcga ccgctgctgg tgagccggcg
     8281 ccatctgacc gaactgaagc gacgattgcg ccgcgtctga acgcttggtc cgtgccgcgc
     8341 cccggcaatg gcgctatcat gcgcgctctg tcgtcaccac cgccgaggag agcccatggc
     8401 gtcgcgcctg ctccgcatcg ccacccgcaa aagcccgctg gccctgtggc aggccaatga
     8461 cgtccgcgcc cgtctgctgg cgctccatcc cggcctgcgg gtcgaactgg tcaccttcac
     8521 cacccgtggc gacaagctgc tcgacgcacc gctggccaag attggcggca aggggctgtt
     8581 cgtcaaggag ctggagcagg cgatcttcga cggcgaggcc gatctggcag tccactcgat
     8641 gaaggatgtg ccgatggagc tgcccgccgg gctgacgatc gccaccctct gcgcccgcga
     8701 agagccggcc gatgcgctgg tcacgccgct gccctgcacg ctgctcgacc tgccgcaggg
     8761 cgcccgcatc ggcacctcga gcctgcgccg gcaggcgcaa ctgcgcgcgc tgcggcccga
     8821 tttcgagctg ctgaccctgc gcggcaacgt caacacccgg ctcgcccggc tggatgaggg
     8881 gcagttcgac gccatcattc tggccgcggc cggcttgcgg cgactcggct tcgcggcgcg
     8941 catccgtgaa tcgctggatg accgtctgct gctgccggcg gccggacagg gcgcagtcgg
     9001 catcgagtgc cgtcaggacg atgtcgcgct gcatgaactg ctggcgccgc tggccgacct
     9061 gccgaccacc ctctgcgtca ccgccgagcg ggcgatgaac cgccgcctcg atggcggctg
     9121 ccaagtaccg atcgccggcc atgcccagct ctcgccacgg ggactgtggc tgcgcggact
     9181 ggtcggcgat gtcgagcgcg gcaccctgct gcgcgccgag cagtggggtg atccggccca
     9241 ggccgaggcc atcggcatcg cggtggcaga gcagctgctg cgcgccggcg ccggcccact
     9301 gatcgaggcg gcacgggtcg atgcagccgc agactaggcc gctcgccggc ctcgatctgt
     9361 gggtcacccg acccgccggg caggccgatc gcctgatcgc actgctgcaa caggcgggcg
     9421 cagccgtgtc tcacctgccg ctgctggcga tcgagccgct gccgccgacg gccgccgccc
     9481 gctcgctgct gctcgacctc gaccgctatc aggcggtgat cttcatcagc agcaatgcgg
     9541 cacggctggg cgccgaggcg atcctcgatc tctggccgca gttgccgacc ggcatcgact
     9601 ggctggcagt gggcgcagcc acccaggcga ccctcgccga gttcgacatc accgcagaga
     9661 ttccagccgg cggcagcgac agcgaggcac tgctggcact gccgcaactc ggcagcgaac
     9721 gggtggccgg tcgggcgatt ctgatcgtgc gtggcaccgg cggccgcgag acgctgcgcg
     9781 agcagttgca gacgcggggt gcccgggtcg actatctgga gctctaccgg cgcatctgcc
     9841 ccgcatcgac cctcgagcag atcaggaact ggagccggcc cctgcccgac ctggtcatcg
     9901 ccagcagtgg cgagggcatc gaccatctgc gccgggcgct gaccggcccg ctgctggcga
     9961 tgctcgagcg gccactgctg ctgccctcga cgcggctggc gcaactggct ggcgagctgg
    10021 ggttccgtca accgctggtg gccgatgacg ccagcgacga tgcgctgctg acctgtcttc
    10081 ttcaatggcg agcccgcaat gccgaaaccc tccttgcctg attcacccga acccgcactg
    10141 ctgggcgctg cccaggtggt gcaggcttcg cacactgcac ccgctgcacg ccctgtctcg
    10201 cgctggccgg cctggctggc gctcggcatt gcgctgctgg gcctgctggc gctggcggcc
    10261 ctgctgctgt ggttgcagcc gcaactgagc acggcatggc agcggctcga ccagttgagc
    10321 gacgcctcca gccggctcga tggtcgcatc gacgggctgc tgcagcagct cgaacagcag
    10381 ggcacggaac aacaggagca ggcggcacgg ctgaccgcac aacaggagca gctcggccag
    10441 cggctcgatg cagtgaccgc cgcccagcag cggttcaacc agggtgatac accacggcag
    10501 tggcggctgg ccgaagcgga gcatctgctg cgactggcca atgaccaact gctcgccggc
    10561 cggccgctcc ggtttgcccg ccagcagttg gcaacggccg accggttggt cgccgaactc
    10621 gacgatccgc agctgcaacc gctgcgcgcc gctctggccg ccgatctggc ggcactcgac
    10681 ggccacgctc cagccgatgt cgaaggccag tttttccgtc tgtcggccgg catcgggcag
    10741 gtcgaccggc tgccgccacg cggcacaccc agcctggccg aggcgacgac cggcgagcca
    10801 gccgcccccg tgcaaccggg ttggcgcggc tggctcgacc gcatctggca gcagttgcgg
    10861 ccactggtgg tgattcgcca gcgcacgcaa tcggtcgaac ccctgctgac gtcgtcgcaa
    10921 acggcgatgc tgcgccagca gttgcagctc cagttgcaac tggcccagct ggcactgctg
    10981 cgcggggagc cgtcgatcta ccacaccagc ctcgatgcgg cgctcgacct gctcgatcgg
    11041 cagtttgacg gcaacgccgc cgaggtgacg gcgctgcgtg cgacgctgcg cgagctcacc
    11101 caacagccga ttgcaacggc agcaccgacg ctggaggctt cgctgtcggc agtgcgtcag
    11161 gccatcgagg ccgagcgaaa cacgccggcc atgacgccaa gcccattccc ggccgaggca
    11221 ccctgacgat gcgtcgtctt ccatggatgc tgctgctggc gctgccgctc accgtactgc
    11281 tggtgctgct ggcgcagcgc gaccccggtt atctgctgct cagttacggc ggcttctcga
    11341 tcgaaaccag cctctggttc gccctgctga gcctgttggt gctgctgtgg ctgctgcggc
    11401 tgttgcagcg gctgctgcgc ggtgggcaac gactcggcag ctggtggcag caccgggatg
    11461 tccgccggca ggcgcaacgg gtcgatgagg gcttgatcgc ctttgccgag ggccagtggc
    11521 ggcaggcgca ccatcggctc tcccggctgg gcggtgcgca ccacgagcgg ttgccgctgc
    11581 gactgctggc ggcgcaggcg gccgaacgcg ccggcgacct gcaggccagc caggcactgc
    11641 tggcatcgat tcgtcaggag gggagcgccg aggatcggct ggcagccgat ctgaccgagg
    11701 cacggctgtg gatcgaccga ggcgcgcccg aacgggccgt ggcgctgctc gatgcgctgg
    11761 gcgcggcaca tcccgaccat ccggcacggc tgcggctgct ggccgaggcg ctgctgcgcc
    11821 agcgggcctg gccactgctg accgaactgc tgccgaagct gaaaaagccc aagctgctga
    11881 acgacgacga gttgcggacg ctggaacgga cgctccagtg cggcctgttc agcgctgccg
    11941 tcgcccaagg tgcagcagcg ctggagcagc agttcacgcg actctcccgg ccactggcgc
    12001 gtgacccaca ggtgattgcg gcctatgtcg atgccctgat cgagagcggt gccgaaccgc
    12061 gtgccgaacg ggcagtgcgc gaagcgctgg gccgtgactg gcaagcggcg ctggtggagc
    12121 gttatggccg catcggtggc gaacatcccg accagcaact ggccaccgcc gagcgctggc
    12181 tgaagagcca tccgcacgat gcaatcctgc tgcgggccgt ggcacggctg gcactgcgca
    12241 accacctgtg gggacgggca cagcaggcat tgcaggccag cttcgcgctg caccccgacc
    12301 cgcagaccgc cgccgaactg gcgcggctcg acacggcgct gggcgaggcc acgaccggaa
    12361 tcggccacta tcagcaggcg ctcgggctgc tggcggtgac gctgccggaa ctgccgctgc
    12421 cgggcaagcc ccgcccatcc tcaggccgac tccccggcca gatcgatcct catcgcacct
    12481 gagcggcaca accgcgcgcg ccacgactgg ccggtgtcgg ccgcgcagaa gatcagcacc
    12541 cgggcaaagc ccatgcatcc atcccaatcg acctgccgca ggtgcggttg cgcctgctgc
    12601 cactgctcga catgcggcgg tggcgcgcac gtcgcgtcgt ccagccagag ccagccggtg
    12661 cacgggctga cctgccgttg cagccgccac tcggccagac tggccgcacc ggcaaagccg
    12721 tggcgatcac aggcgatcag cagtgaatcg gcttcgtgca gccgcccggc gtgccactca
    12781 cccaccggcc agtgacagcg aatcaccggc tcgcgacgca gccagtcgaa cagcgacggc
    12841 cacagatcgc ccagcggcag cgggtcgagc aggaattcga gcagccgccc atccggccat
    12901 ggggcgcgaa aatggagctc gacctgccga tcggcataga tcagtcgcgc ccgttgcccg
    12961 gcgaaatagc gcggcagacc gcccgcggcc ggagtgcgca gcagcagccg ccaacagcca
    13021 tccgcagtcg gatcgagcgc cgcgacctgg caggcgcgct ggacctgagg cagggtggac
    13081 ggcggcagca gcgccgggtt gtcgagcagc agatcgctca gcgcgatggc gcagcagggc
    13141 aggatggcac aggaggttcc gtccgcttcg atgatgccct gcgcggtcaa caccctgccg
    13201 cgcaacagtt gcgaacggca gcgctcacag ttgccgttgc gacaggaggc cggcaggctg
    13261 aagccatgcc gctgcgcggc ggcgagcagg gtttcgttgt cggtggcggg gtgtcgatgg
    13321 tcgggttgcc agcagagggt gtgcagcatc gcaaccaccc tgcctcagtc gatgccgagt
    13381 tcgggccaga gctggtcgat ccgtgccttg accgcggcat ccatccggat cggccgtccc
    13441 cactcccgct gcgtctcgcc cggccatttg tgggtggcat cgagccccat cttgccgcca
    13501 agaccgctga ccggcgaggc gaaatcgagg tagtcgatcg gggtctggtc gatcagcagg
    13561 gtgtcgcgca ccggatccat ccgggtcgtc accgcccaga tcacctcctt ccagtcgcgg
    13621 gcatcgatgt cgtcgtcggt gacgatcaca aacttggtgt agatgaactg ccgcagaaac
    13681 gaccagaccc cgagcatgat gcggcgggca tggccgggat agcccttctt gatggtgacc
    13741 accgccagcc ggtaggagca gccctccggc ggcagataga agtcgatgat ctccggaaac
    13801 tgcttgcgca gcagtggcac caccacctca ttgagcgcga cgccgagaat ggccggctca
    13861 tcgggcggct tgccggtgta ggtgctgtga tagatcggcc gttcgcgctg ggtgatgcgc
    13921 tcgatggtga ggaccggaaa acgctcgacc tcgttgtagt aaccggtgtg atcgccaaag
    13981 gggccctcat cggcgaagtc gccgggatgg atcaccccct ccagcacgat ctcggcactg
    14041 gccggcacct gcaggtcgct gccgatgcag ttgaccagct cactgcgctc accacgcagc
    14101 aggccggcga aggcatactc cgaaagggtg tcgggaatcg gcgtcactgc cgccaggatg
    14161 gtggccggat cggcacccag cgcgaccgcc accggaaagg gccggtcagg atggcgctgc
    14221 cagaattcgc gcagatcctg cgcgccaccc cgctgggcca gccagcgcat gatgacacgg
    14281 ttgcggtcga tcacctgctg acggtagatg ccgaggttct gccgcggctt ttcgggtccg
    14341 cgggtcacca ccagtggcca ggtgatcagc ggtgccgcat cgccgggcca gcaggtctgg
    14401 atcggcagcc gccccagatc gacctcgctg ccctcgaaga tctggctctg gcagggggcg
    14461 gtgcgggtca gcttcggcgc caggttgagt gcctgcttca gcagcggcca cttctcccaa
    14521 gcatccttca gaccgcgggg cggctcgggc tcgcgcagcg ccgccagtgt ctcgccgatc
    14581 gcacgcagcc ggctcggctc ctcctccccc atgccgagcg cgacccgctg caccgtgccg
    14641 aacaggttgc tcagaagcgg catcgagtgg ttcagcggct gctcgaacag cagcgccgga
    14701 ccgccgcgca acagggtgcg ctggctgatt tcggtcactt cgaggcgggg gtcgaccggt
    14761 gtcctgatcc gcttcagctc gccccgctgt tcgagcaggg cgatgaagtc gcgcaggtcg
    14821 cggtagcgca tggccgttac gccatcactt gcgtttcatc gaggcgaaga actcatcgtt
    14881 gctcttggtc tgccgcagct tgtcgaccag gaactcgatc gcatccaggt catccatcga
    14941 attgagcagc ttgcgcagga tccacatccg ttgcagttcg tcctcgctgg tgaggaactc
    15001 ctcgcggcgg gtgccggagc ggcggatgtt gatggccgga tagatgcgct tctcggcagc
    15061 acggcggtca aggtggattt cggcgttgcc ggtgcccttg aactcctcgt agatcacctc
    15121 gtccatcttg gagccggtgt cgatcagcgc cgaggcgatg atggtgaggc tgcccccctc
    15181 ctcgatgttg cgcgcggcgc cgaagaagcg cttgggctgg gccagcgcgt tggcatcgac
    15241 accgccggtc agcaccttgc ccgaggaggg caccacggtg ttgtaggcgc gggccagccg
    15301 ggtcatcgag tcgagcagga tcaccacatc catccgatgc tcgaccagcc gcttggcctt
    15361 ctcgatgacc atctccgcca cctgcacatg gcgggtcggc ggctcgtcga aggtgctggc
    15421 gatcacttcg ccacgcacca tgcgctgcat ctcggtcacc tcctcgggac gctcgtcgat
    15481 cagcagcacg atcagatagc attcggggtt gttgcgggcg atcgactggg cgatgttctg
    15541 cagcaggatg gtcttgccgg ccttgggtgg cgagacgatc agcgcgcgct gccccttgcc
    15601 gatcggcgag accagatcga tgatgcgggc ggagatgtcc tcggtgctgc cgtttccggc
    15661 ctccagcagc agccgccgct gcggaaacag cggtgtgagg ttctcgaaca ggatcttgtt
    15721 cttcgaactg tcgggggtgt cgtagttgat ggtgtcgacc ttgagcagcg caaagtagcg
    15781 ctcaccctcc ttgggcggac gaatcttgcc caccaccgta tcgccggtgc gcaggttgaa
    15841 gcggcgaatc tgactcggcg aaacatagat gtcgtcggga ccggccagat aggagccctc
    15901 cgccgcccgc agaaagccaa agccatcctg caggatctcc agcactccat cgccgctgat
    15961 gtcctcacca ctgcgcgcat gtttcttcaa aatggcgaag atgatgtcct gcttgcgcga
    16021 gcgggccatg ttctccagcc ccatctcgtt ggcaatctgc agcagttctg gaatgggttt
    16081 ggtcttgagt tcggtcaggt tcatgtgcgg gcgtgttggc tggtgactgt ggctggcggg
    16141 ttgttgcatg gtcgaagcgt gatgagggtt gagctgtggg ctattgagcc gaggcgtggt
    16201 gcctggccag cggaaacatg gcaaagataa aagatagaag aggacgaatg gactgcgggt
    16261 gtgcagactg gacgaacccg aggtggcgaa tgtgatcgcc cgaaagaccg ggatgggtct
    16321 gttccgatgc ctgggtcaca cagaaagcgg cgcctcagcg gcgcgctcct tctgcctaat
    16381 ttagagccga ttgcaaaaaa agcaagagat gcgttgcgcg gagccggttt tcgtcatcgc
    16441 tgaaggcggt cgcaccgggt ggattggcgc cgacaggggg agtagaatcg gccacctttt
    16501 gtcaggcaag ggtacccacc tttgatgtca tccgatgctc gcaccagcca tttcgctgca
    16561 atgtgaagcc atgactcttt tctcgccgct tgtcagcctg gccattgcgg tcacggcaac
    16621 gacacctttc atcagcaggt gacatccgtg gctgattcca gttcgatccc ttcatccacc
    16681 gaaatgcacg cttccgaacg cggaccggaa aaccgcgaca gccgactgat ggtgctcagt
    16741 ctggcggcac tgggggtggt gttcggcgac atcggcacca gtccactcta cgcattgcgg
    16801 gagtgctttt tcggcagcac ccagctgccg gtcaacgagg ccaatgtgct gggcgtgctg
    16861 tcgctgatct tctggaccct gatctgcgtg atctcgatca agtatcagac cgtggtgctc
    16921 aaggccgaca accgcggcga aggcggcatc ctggcgctgc tggcactgct ggacccctgg
    16981 cactcatcac agggcggcaa caaacccaag acgctggtga tcctggcgat catcggcgcc
    17041 tctttgctct acggtgaagg ggtgatcacg ccggccatct cggtgctgag cgcggtggag
    17101 gggctggagg tggcggcacc gggtctggcg cactacgtga taccgatcac cgtggtcatt
    17161 ctgatcgggg tgttcctgct gcaaagtggc ggcacccaca aggtgggggc gctgttcggt
    17221 ccggtgatgg tggtctggtt cagcgtgctg acaatcctcg gcctgctggc catcgggcgc
    17281 aatccggcgg tgttggcggc actcaaccca ctgcacgcgg tgaatttttt catcgacaac
    17341 aaaatggtcg gctttttcac gctcggcgcg gtctttctgg tggtgaccgg cggtgaagcg
    17401 ctctatgtcg atctgggcca ctttggccgc aaaccgatcc ggcgggtctg gttctttctg
    17461 gttcttccag ccctggtgat caactaccta gggcaggggg cactgctgct gacgagtggc
    17521 ggcgcaatca aggacccctt cttcgcactg gcgccggagt ggggtctcta tccgctgatc
    17581 atcctggcga ccatggccac cgtgatcgcc tcgcaggcgg tgatctcggg cgtcttctcg
    17641 ctcaccaacc aggcgatcca gctgggtcag gcaccacgga tgaatgtggt ccagacctcg
    17701 ccgaacgaga ttggccagat ctacatcccc tttctcaact gggtgatgat gctgaccacc
    17761 atcgcactgg tgctcggctt caagtcatcg agcaatctga tcagcgccta tggcatctcg
    17821 atcagcaccg cgatgctgat cacctcgctg ctgaccttct tcgtgatgtc cgagaagtgg
    17881 caatggccgc gcccggcggc actggccatt gccgggctgt ttgcgctgat cgacctgtcg
    17941 ttcttcagtg ccaacgtggt caagatcgaa catggcggct ggttctcggt gctggtggca
    18001 ctgctggtct acacgctgat gtcgacctgg agctccggac gacaggcggt gatcaaccag
    18061 ttgcgcggct cggtcgcccc cctcgatgag ttcctgagcc atttcacggc gcagtggccg
    18121 ctgcgggtac cgggcaccgc catcttcatg accgcgcctg atctgggcgt gccgccgatt
    18181 ctgcaatacc acctgatgca caaccaggtg ctgcatgagc gggtgatcct gctgacggtg
    18241 gtgaccgagg atgaaccctg ggtgccagcc tcgaagcggc tgaagatcgt cgaactcagc
    18301 cacggcttct accgggtgca tgtgacctac ggcttcatgc agagccccaa tctgccggtg
    18361 gcgctgcgcc tcggcgaacg actgggtctg accttcgatc cggaggccac cagctactac
    18421 ctgggacgtg agacgctgac gccgcacggc tcggtctcgc gcatgatgcg ctggcggcag
    18481 atgctgttcg ccgtcatggc gcgcaatgcc gtgcgcgcca ccgacttctt caaactgccc
    18541 ccctaccgca ccgtcgaact cggcatgcgg ctcgaactga tgccgatggc ctatctcgga
    18601 ctgaagcccc acaagaaaaa tggcaacggc aaacagagcg agccagacgc ggccgctgcc
    18661 ccggcggact ccagcatcgt gccatgaccg cagcggcgga gcggccggcc gcgctccgcc
    18721 atcccattct gcttgcttgc gacgccaccg cttatcactt agtcttcggc cggttccaaa
    18781 cggcagccat gcctgtcgat gcgccgttgg tgaacgggat gaatggactt tccgcttgcg
    18841 gtaaagaaaa cagcctaaag acaagatcag ttaaggaagg caacacatgg cctatgattc
    18901 gacgtacatg cgcggcaagg ccgcgatcgt gggggcggtc gacgccgtct ccccaaccgg
    18961 cgagctgggg attcacgagc gcaccctgca ggtgcagatg atccatgcgg cactggaaga
    19021 tgccgggctc agactctccg atgtggatgc gctcttcgcc accggcacca tgatgtcgtc
    19081 gatggagctg gccgagcatc tgatgatcac gccacgctac accgacacca ccatgaccgg
    19141 cggctccagc ttcgaggtgc atgtcgaaca tgccgccgcc gcgattgcac tgggactgtg
    19201 cgaagtggca gtgatcgtct atgccgccac gccgcgcgcc aagggtggcg ccaacttcat
    19261 ggcagcgcag atggccgcgc tcgacaagaa caacccgccc cccaacatcg agtgggagct
    19321 gccctacggc accatctcgc caccgggcaa ctatgcgctg gccgccagcc gccacatggc
    19381 gctctacggc accacccgcg cgcaactggc gcagatcgcc gtctcgaccc gtgagtgggc
    19441 ctgcaagaat ccgcgtgccc gccatcagga gccgctgacc ctcaaggagg tgctcgaagc
    19501 accgatgatc gcatcgccgc tcggcaagct cgactgctgc ctggtcaccg acggtgccgg
    19561 cgcactggtg atgacctcgg ccgagcgggc ccgcagcctg cgcaagaagc ccgcctatgt
    19621 gctcggcgcc ggcacggccc acgaccacgc gatgatgatc agccagatgc ccgacctctg
    19681 caccaccgcc ggcgcgatct ccggtcccaa ggcgttcaaa atggccggca tcacccccaa
    19741 ggatgtcgat gtcgtggaga tctacgactc cttcaccatc accgtggcgc tggcgctcga
    19801 agacctcggc ttctgcaaga aaggtgaagc cggcgccttc gtccaggatg gcaggctggg
    19861 gcccggtggc gccctgccca ccaacaccaa tggcggcgga ctctcctaca cccatccggg
    19921 catgtacggc atgttcctgc tggtcgaggc ggtcaagcag attcgtggcg agagcactgg
    19981 ccaggtcaag gatgcgaaga tcgcggtggc gcacggctgc ggcggcattc tgtcatcgac
    20041 ttccactgtg gtattgggtt cggaggagac actatgaaac ggatgatcct gggtacatca
    20101 ccggttgcag cccccttctg ggaggccacc aaggagaaga aattcctgct gcaattctgc
    20161 gccaccaccg gcaaggcgat ctggtatccg cgcgacttct cgccccatgc gctgggcggc
    20221 aacgtcgagt ggcgcgaagc cagcggcaaa ggggtggtct atgcggtcag cgtcatgcat
    20281 cgtcccggca atccggcgat ggccgaagcc gtgccctatg ccgtcgcgct gatcgatctc
    20341 gaggaaggcg cgcggatgct cagcaatgtc gtcaactgcg atcctgattc gatcaaggtg
    20401 ggcgacaagg tcaagctggc ctgggagccg ctggagggcg ggcggcatct gccggtgttc
    20461 gaactggcct gacgcgcact gcctgcatca ccgcaaaccc cggttcatgc cggggtttgt
    20521 cgtttcagaa caatcgtcga cgttcccggc agaccggcga tccagtgcag catcctcgtc
    20581 aggacggccg cgccttcacg ctgtcgaaaa aaaaccctct gcatggcgtt tccatgcaga
    20641 gggcacagtg gtcagatcaa gagctcaacg ggacgcaccc ggatcaggcg ctcttgtcga
    20701 acagcggctt ctccagttcg acccagtcga tctcatggtt gtccgggtag gtcgaatagg
    20761 tcagcagacc caactggtca tacttcgacc ggatcttgtc ggtcagcagg ccgacccggt
    20821 tcaggttcgg catcacccgg gtgaagagca gattgcggaa gttgccggcc acatcggagt
    20881 cgagctgcca tttgcgggcc tcctcgggat cccagccgaa ctgcgcaaag acatcgctgg
    20941 tgatcagccg ttcgcgcatc agcacgcaga catcgaaggc aatcgccgcg gcatcctcgc
    21001 gctcctgggt cggcagggtc ttgacgaagt cctcgaggaa attgacgcca aaggtgacat
    21061 ggcgcgcttc gtcacggatc accagatggc agacctgggc gaacagcgga tcgacggcgg
    21121 acatcttggc gctgttgaag gccgccagcg ccagcccctc gatgatcagc tgcatgccga
    21181 tgaacttgag ataccagcgc tcatcgccca gcaccttgtc gagaatcgcc ttcagcgtct
    21241 cgctgatcgg atacatgaag ccgatcttgc gccgcaggta tttgttgaac acctcgacat
    21301 ggcgggcctc atcgaaggtc tgggacgagg catagagctt ggcctcatag gtgggcgcgc
    21361 agctgaccag ctgcgaggcc accagcatgg cgccctgctc accatgcagg aagttgctgc
    21421 atttctgcgc catcatgtgc cagtcgaatt cgatcttctt ctcttccgac atcgcttcat
    21481 aaggcgcaaa gccacggaag gcattgtact tctggtcggt gatgaacttg gtccggtcga
    21541 ccggggtgtc ccagtccaga tcatcctcgg cattccagtt gagctgtttg ccgagctcgt
    21601 agagccgctc cagccggtca ttggtgatgc tgtagtccca gttgtaggag ccgaccagcg
    21661 gcgtgtggaa gatctcccgg atgatctcga tctcgttcgg tcccagtctg gcgttgatct
    21721 cttcctgctg atgcaggtca atcaggtcac gatgcatctt ctctacgatc ttgacgttgg
    21781 gcatggcagt actccggcgt ggaaagtgtg gtgtgatggc tgcgaccgcc actcatccac
    21841 gctgtggcac atcgcctgac cataagacca ttcgtattga cccacgtcaa gcgcccgaga
    21901 cattcactca gccgcacccg gttcgccttt gaccgcagcc accagattct cgaacggcac
    21961 catgccggga atcagctgcc catcggccag aatgatggcc ggcgtgccct gcacgccgag
    22021 cttcagcccc agcgcatact ccttggccac cggattgctg caacgggcgg tcggcacctt
    22081 cttgccggcc ttggcatcgg tgaaggcctg ctgccgctcc ttggcacacc agaccgacac
    22141 catcttgtca tgggtggcgg agctgagccc ctcgcgcggg aaggccaggt agcgcacctc
    22201 gatgcccgcc ttgttgagcg ccggcaccat cttgtgcagc tcgcggcagg caccacagtc
    22261 gacatcgctg aagatggtga tggcgtgatc ggagggctgc cccggaaaga tcaccatctc
    22321 ctccggtttg atctcactca gcaacttgac gcgctcctgg gcacgcccct cctcgctgac
    22381 attgatcgcg ccggcaccgg tcagccggta gaggtcgccg acgatgaagt ggctgccatc
    22441 ggccgtgctg tagagcaccg ggccctgctc ggtggtgacc cggtagatcc cggtgatcgg
    22501 gctcgggctg atctcggcaa tgcgcactct gggcacaccc ttttcgatgg cggcccggat
    22561 ggtcgcctcg gcagtggatt cggccagcac ggcctggatg ccaagcatcg acccgcacag
    22621 cagcagcacc cacgacaatc gtctgatcat cactttcatc tcctgaagtt gttcaacacg
    22681 gttcgcatca gccgcggcgt ggcgtgcggg ccacggccca cacctccacc tcgatcgcgc
    22741 cggcacggcg caggacgcgg ctcatcgcct gcgccgtcgc gccggtggtg accacgtcat
    22801 ccaccactgc cacccgccgg ccggccagca accctttgac cacgaaggcg tcacgcaggt
    22861 tgcgttgccg cgccttgaaa tcgagctggc gctggtcgac acccgagcca cgctgtttca
    22921 gcagatggca atcgacctcg atgccaagtc gtcgacccag accgaccgcc agctcctgcg
    22981 cctgattgaa gccacgctga cgcagccggc ggggatggct cggcaccggc aacagcagct
    23041 cgggtggcgg cgcgctggcc agccgcccga cgcgtagatg gctggccaac agatcggcga
    23101 acagccggcc gagtgccggt tgcggctgct gcttccagtc gcgcagcaac tggtcgagcg
    23161 gcggcgcgaa ggccagcggc gccaccactt ggctgaaggg gggctcctgc gcctgacagc
    23221 ggccgcagag cagttcggcc gccggcagcg gcagtgcaca gcggcggcag ccatgtgtga
    23281 tccatggcag ttcggcctcg cagggtgcgc agaggtcgag cggccggttg ctgcccattg
    23341 cgcagagcag gcagtggccc ggcagcagcg cgaattgcag ccgatcgagc gattggtaaa
    23401 ccgccctcat ccacctgggt tgacaattct gccgccactg ccgtaccgtc atcggccact
    23461 gcgcaggctg cggtcgtcgc acgcgcatcg aacagactcc attttcgtca ccctggtcaa
    23521 ggaacccgcc atgcacagcc ccgccgccca ccccatccgc catgactgga gccgcgggga
    23581 ggtcgaagca ctcttcgccc tgccattcaa cgatctgatc ttcagggcgc agagcctgca
    23641 ccggcagttc catgatccca accaggtgca gatcagcacc ctgctgtcga tcaagaccgg
    23701 tgcctgcccc gaggattgcg cctactgccc acaaagcggg caccatgata ccagcctcga
    23761 gaaagagaaa ctgctggagc tggagcgcgt cgtcgaggcc gcccgcaacg cccgcgacaa
    23821 gggcgccacc cgcttctgca tgggcgccgc ctggcgctcg ccgcatgagc gcgacatgcc
    23881 ccatgtgctg tcgatggtgc gggcggtgaa ggggctcggc atggagacct gcatgaccct
    23941 cggcatgctg accgggccgc aggcgcaaca gctggccgag gccggactcg actactacaa
    24001 ccacaacctc gacacctcgc cggagtacta cggcgccatc atcaccaccc gcacctatca
    24061 ggaccggctc gacacgctgg cccatgtgcg cgaggccggc gtcaacatct gcagcggcgg
    24121 catcgtcggc atgggcgagg cggaacgtga ccgcgccggc ctgttgcagc agctcgccaa
    24181 cctgccacaa catcccgaga gcgtgccgat caacctgctg gtgcgggtcg agggcacgcc
    24241 gctggcgcag gtcgatgcgc tcgacccctt cgacctggtg cgcaccatcg ccgtggcgcg
    24301 cattctgatg ccggccgcgc aggtgcgcct ctccgccggc cgcgagggga tggaggatgg
    24361 catccaggcc ctctgcttcc tggccggtgc caactcgatc ttcgtcggcg agaagctgct
    24421 caccaccccg ctgcccgaga ccagcgccga cctgaagctg ttcgcccggc tcggcattcg
    24481 tcccgaagcc cgcaccgagt cggccgacaa cctggctgcc actgcggcgc tcatcgatgc
    24541 cgcgcagcgc accgcgaacg acgaccgttt ctacgacgcc atgtcaccct ctgcccactg
    24601 acgtcacggc catgaacccc ctgctggagg cgctgacaca gcggctcgaa acccgtgtcg
    24661 ctcagcagct ctaccggcag cggcgtgtca ccgactcacc gcagcagccg ctgctcaagc
    24721 aggatggccg tgagctgctc gccttttcga gcaacgacta cctggggctg gccaaccatc
    24781 cgcgggtcat cgaggccttc accgccgccg tccgccagta tggcgtcggc agcggcgcct
    24841 cccacctgat caatggccat ctgcgcccgc atcaccagct cgaagaggcg ctggccgact
    24901 tcaccggccg cgagcgggca gtgctgttct cgaccggcta ccaggccaac ctcggcaccc
    24961 tgagcggact gctcggccgc cacgaccgga tctatcagga ccgcctcaac cacgcctcgc
    25021 tgatcgatgg cgccacattg agccgggcca gactgctgcg ctatcgccat gccgacagcg
    25081 gccatctggc cgaactgctc tcgcgccggc cgccggccac caccgccctg atcgccaccg
    25141 acggcgtctt cagcatggat ggcgatctgg cgccgctgcc gcaactggcg cggctggcca
    25201 gcgaccatgg cgcctggctg atggtcgacg acgcccatgg catcggtgtg ctgggcgagc
    25261 agggcggcgg cagcctcgaa cggttcgggc tggggcaggc gcaggtgccg atcctgatgg
    25321 gcaccctcgg caaggcactc ggtacctttg gcgccttcgt cgccggtgcc gcgcccttga
    25381 tcgagaccct gatcaacact gcccgaccct acatctacac caccgcgctg ccaccggcgg
    25441 tggcagcggc gagctgcgtc agcctcgaac tgctgcgtca agaggggtgg cggcgggagc
    25501 ggttgcggca gttgatcgac caactgcgcg ccggtgtcgc cagccttggt ctgccgctga
    25561 tgccgtccac cacgccgatt cagccgctgc tgctggggtc gagcagcact gcactggcct
    25621 ggagtcaggc gctggaggcg cagggcattc tgatcaccgc gatccggccg ccgacggtgc
    25681 cgcagggcag cgcccgactg cggatcaccc tgagcgccgc ccaccagcca cagcagatcg
    25741 accggctgct ggaggcgctg gacaaggtca ggcgacagct cggcgcgccc gaactcgcgc
    25801 aggccaaccc atgaaccctc caccctccgc cgagacagtg gccgcagcgg ccgcgccgcc
    25861 gatcgacaag gggcgcatcg cccgctcctt cggccgcgcc gccggcagct acgacagcgt
    25921 cgccgacctg caacgccacg ccatccagcg gctgatcgag cagttgccgg ccgatctgac
    25981 gccacagcgg atcgtcgatg gcggctccgg caccggcctt ggcgcgctgg cactacgcga
    26041 acgctacccg caggcgcaac tgacattgct cgatctggcg ccggcgatgg tccgtcatgc
    26101 ccgtgcccgc ccgccgttgc aggctgccca gcacctctgc gccgacctcg aagccgtgcc
    26161 gctggccagc ggttcggtcg agctgctgtt ctccagcctg gcgctgcagt ggttgccacg
    26221 gctcgatctt tttctcgacg aagcctggcg actgctggcc cccggcggct ggctgctgtt
    26281 ctcaacactg gggccggcca cgctgcagga gctgcgcggc gcctgggcca gcgtcgatgc
    26341 caacgtccat gtcaaccgct ttctgccggt cgatcagatc gaaaccgccc tcggccgcca
    26401 cccgtggcgg cagcgccggc tgattccggc gccgctggtc agacactacc ccagtctgcg
    26461 cgcgctgacc catgagttga aggcgctcgg cgcccacaac ctcaatccgg accagccgac
    26521 cgctctgacc ggtcgccagc gcatcacccg cgtgagcgcg cactacgaaa ccctgcgcac
    26581 cgcaacaggg ctgcccgcca gttggcaact gctgctgggg gtgctgcaac ggtgaagcgg
    26641 gcctacttcg tcaccggcac cgacaccgat gccggcaaga cgctggtgtc gagcgcgctg
    26701 ctgctgcgtg cccgccagca aggctggcag accgccgcgg tcaagccggt ggccaccggc
    26761 ggtctgccgc accagggcgt gctgaagaac gacgacgcgc tgcaactgca gcgacagagc
    26821 acactgccgc tcgactatgc gctggtcaat ccggtctgcc tgcatgatcc gatcgcgccg
    26881 cacatcgccg cccaggaggc cggcatcgcg ctgcacgcag cactgctcgc cactgcctgc
    26941 cgccgggtgc tggcgctggg cgccgacttc acgctgatcg agggtgccgg cggctggcgg
    27001 gtgccgctca atgaccgcga gaccctggcc gatctggccc gcctgctcgg cctgccggtg
    27061 atcctggtgg tcaacatgaa gctcggctgc atcaaccatg cgctgctgag ctgcgaagcc
    27121 atcgccgccg atggcctgcc actggccggc tgggtcgcca accgtgccgc accgcagatg
    27181 agccgcttcg agcagaacct cgccagcctc gaccggctgc tgccggcgcc ccggctgggc
    27241 atggtgcccc acctgacgcc ggtcagcgcc gaacaggccg ccccctggct gcggctgccc
    27301 gactgaccgc gtccttcagc ccgagaactg ccgtcgcagc atctcgattc gttcgcggtt
    27361 ggtgccgagg tcggagcgac cgacccgcga cgcagatcgc acctcgatcc gtcccggcgt
    27421 gatgaacaga aactccacat catcgacaaa gcgcagcagg cgggtggtga actcggcatg
    27481 gaggtaggtg ggcgagaccg tcaccagccg aacccgcggc tggctgccaa gcaactgctc
    27541 cagccgggcg cgcgcctgct ccgtgctgcc ggagtagctc agcggtgcga tggcatgttc
    27601 ggcgtcggcg gccgccgcct cgctcgacac acagttgggg ctctgcggac agggatgcag
    27661 gtggttgtcg accacgccga tgttttgcgg tggcggggcc gaacagccgc ccagcagcgg
    27721 cagacagagc agggcgagcc gagtggcatg tcgcgggcgc atggcggact cctcattggt
    27781 gcactggaac tgccgcacac tataaccggc cggcgcgcct ggcccgcatc gatggattgt
    27841 cgccggcgtc atcgctgtgt aacagtagcc gcgccctgtc caaccgctgc gaggtccgca
    27901 tggaacttcc ccacttcaac gacggcctgt tccgctatct gcgccgctca ccgaccccct
    27961 ttcatgccgt ggccgaagcg gtacggctgt tggtcgaacg cggcttcacg ccgctgaacg
    28021 aggccgacgg ctggtcgctg acgccggggc agcgctatct ggtcacccgc aacggctcgt
    28081 cgctgatcgc cttcgtgctc gggcagcgca gtccgctgga gtcgggcatc cgcatggtcg
    28141 gcgcccacac cgacagcccc tgcctgcggg tgaagccgga gccggtgctg cgctcacagg
    28201 gctgcctgca actgggcgtc gaactctacg gcggcgtgct gctgaacccc tggttcgacc
    28261 gcgacctgtc gctggccggc cgcgccacct gcctcaccac ccgtggcgag ctggtcaacc
    28321 tgctgatcga tctgaaaaaa ccgctggcgg tcattcccag cctggcgatt cacctcgacc
    28381 gcgacgccaa cagccagcgc agcatcaacg cccagaacga cctgccaccg atcctgctgc
    28441 aactcgacga caggcagagc ttctccttca cgctctggct gcgcgaacag ttgcaactgg
    28501 cccatcccga ggtcgaaatc ggcgaggtgc tgggccacga actgagcttc tacgacaccc
    28561 agccccccgc ctatgtcggc tgggatgacc agttcatcgc cagcgcgcgg ctcgacaacc
    28621 tgctgagctg ccacgtcgcc ctcgccgcca tcgccgaggc cggcagcgaa ctcagcacgc
    28681 tgatcgtcct caatgaccac gaagaggtcg gcagcggctc ggcggccggc gcccaggggc
    28741 ccttcctgcg cgcggtgctg gagcggatgg tgccgggcag cgaagccctg acccgcgcca
    28801 tcgaccgctc gctgttcgtc tcggccgaca acgcccacgg catccatccc aaccatgccg
    28861 ccaagcatga cgccaaccac ggcccgaggc tcaaccaggg tccggtcatc aagttcaacg
    28921 ccagccagcg ctacgccagc aacagcgaaa ccgccgcctg gttccgccac ctctgcaccc
    28981 agagccgggt gccggtgcag agcttcgccg tgcgcagcga catgggctgc ggcagcacca
    29041 tcggcccact gaccgcggcc gagattggcg tgcgcaccgt cgatgtcggc gtgcccacct
    29101 ttgcaatgca ctcgatccgc gaactggccg gccgcagcga cgcccaccac ctctaccggg
    29161 tgatcaagcg gctattcgcg accgaacggc tgtcgtagcc gcgctacagg cagtgggtgg
    29221 gcttgggcag cccggcgatc ttggccgcca gcttggccgg actgcccgga aagagccgca
    29281 gcagatgaat gctgcgcccc tgctccggcc ccagctcgcg ggcaatgaag ttgaccagcg
    29341 gccgcatcgc cggtgactgc tggtagcggc ggtagaactc acgcagcagg tggatgatct
    29401 gccaatgctc ggcggtcagg gtgatgccct cggccagcgc gatctgctgc gccaccgcct
    29461 cgctccacgc atcgagccgg cacagatagc cctcggcatc ggtttcgacc gccatggtga
    29521 ccctgctcac gctctccgac tgctgcatgg cacccattct aggcgttttc gccccctctg
    29581 ctcacctgcc acgctgacca gaaaaagtgt caacattttt tacaaaatca tgaataaaaa
    29641 gccaatccac acgcacgctc aaacaattta tgtttaaaaa acaaaaacat gacaactgtg
    29701 gcacgatttt tatatggaaa atgtgcccag agacagaaac agcgcacttg ctgacaattc
    29761 cactcactga aagcgagaac gatcagatga aaacaaaacc gccacatccc ttcacgccac
    29821 gtctgctgag cctgatggtc gccgtggcct ttcttccctc cgtaccggcc atggccgcca
    29881 attgcgtctg gaacccagcc gccgggaatt gggccactgc cggcaactgg tcctgcgcac
    29941 aggtgccagg cagcgctgac agtgccggca ttgccggcgg ccagactgtc atggtcaact
    30001 cggcacaatc gatcttcaat ctgaacaacg ccggcagcct caacattgac atctttctgc
    30061 tgaccctgaa cggcggcggt gccacccgca acagtggggc catcaatgtc ggcagcggag
    30121 cgatcccgtt caatgccgca ctgcaggtcg ggggtgggca caacatcgac aacagcggtg
    30181 gcaccatcaa tatctcggct gacagcgtgc tcaaccagtt tggcagcacg atcagcggcg
    30241 gcagcatcaa cacctttggc agcggcaagg tcgtcgcctt caacagcaac agcaatgccc
    30301 tcagcgctgt caccctcaat ggcaacctcg atctggcgag tgcgaccggt gtcgagcggg
    30361 tgttcggtgg cctgacgctc aatggcgtca tcgacctcaa cagcaacagc gtgctgtcgt
    30421 tcggcggtga ccagaccctg ggcggcaccg gcagcgtggt ccttggcgac tctggcagca
    30481 gcaaccgtgc cctgagcctg gagggtgaca ccaccctcac catcggctcg gacatggtgg
    30541 tacgcggcaa gcatggcacc ctcggacagg cctacctctc cggcggctcc cagaagctgg
    30601 tcaatgatgg ccgcatcgct gctgacgtca gtggcggcat tctctatctc gccccttcgc
    30661 acggcaccac caacaacgga gtgatcgagg cgcagaatgg cggcaccctg gtgctcaaca
    30721 gcaatgttgc cggaaccacc ggcagcgccc tcaatgccgg tgccggcagc ctgattctgc
    30781 agaacggcgt cacgctgagc ggggtgatca acaccagcgg cagcggcaac ctgcgggcca
    30841 gcaacagcaa cagcaatgcc ctcagcgctg tcaccctcaa tggcaacctc gatctggcga
    30901 gtgcgaccgg tgtcgagcgg gtgttcggtg gcctgacgct caatggcgtc atcgacctca
    30961 acagcaacag cgtgctgtcg ttcggcggtg accagaccct gggcggcacc ggcagcgtgg
    31021 tccttggcga ctctggcagc agcaaccgtg ccctgagcct ggagggtgac accaccctca
    31081 ccatcggctc ggacatggtg gtacgcggca agcatggcac cctcggacag gcctacctct
    31141 ccggcggctc ccagaagctg gtcaatgatg gccgcatcgc tgctgacgtc agtggcggca
    31201 ttctctatct cgccccttcg cacggcacca ccaacaacgg agtgatcagc acggtggctg
    31261 gcagtacggt gtcgatcggc aacagcttcc tcaatgccga aaccgggctc atcaccggca
    31321 atggcaccgt ggtggcaccc agcggcggtc tgaccaacgc cggccatgtg gcaccgggcg
    31381 cttcgccagg cacgctgacc atcgctggca gctatgtaca gaccacggcg ggcaccctcg
    31441 acatcgaact ggctgatttg agcagcttcg acctgctgaa ggtgaccggc aacgccacgc
    31501 tggccggcac gctggcgctg cactgctttg gcgcctgcaa cttcgcggtc gatgacacgc
    31561 tgaagattct cgacgccgcc gacaacgcca gcagtctgac cggcagcttc gacagtctgc
    31621 tgctgagcgg ctttggctcc ggccagttcg acgtcatcta cgaccgggcg ctgggcgatg
    31681 tgctgctgaa ggtgacccag gcagtcaccc cggcaccggt gccactgccg gccgcctcct
    31741 ggctgatgct cggcggcctt ggcctgcttg gcggcctgcg ccgggtgaag cgcgccggtt
    31801 gatcggttcc atcccccctc ccggtcttgc gcacctcagg caaggccggg aggggcaccc
    31861 cgcttcccgt ccctgtcgcg cgctgcgcac cacggccgac tgccccgcac cggcccgatc
    31921 aaatccaccc gaagggtgta caatcgcgta gcctccactt ttgcgtgatc cgccatgtcc
    31981 cacctcgacc ctgctgccga cgagggcagc cagaccaccg atttcggtta ccagcaggtg
    32041 ccgctgcgcc agaagcgggc gcgggtcgcc gaggtgttcg actcggtggc cggtcgttat
    32101 gacctgatga atgacctgat gtcgttcggc atccaccggc tgtggaagcg gctgacggtg
    32161 gaggtgagcg cgctgcggcc gggcgactgg gcgctcgatg tcgccgccgg taccggcgat
    32221 ctgaccgatc tgatgagccg caaggtgggc cgccatggcc gggtggtgat gagcgacatc
    32281 aacagcgcga tgctgcaacg gggccgcgac cgcctgctcg accgtggcgt ggccggcaat
    32341 gtcgactggc tgatcagcga tgccgaagcg ctgccctttg ccgacgacag ctttgccgtc
    32401 gtcaccatcg gcttcggttt gcgcaatgtc accgacaagg cgcgggcgct ggccgagatg
    32461 gcgcgcattc tgaagccggg cgggcggctg ctggtgctgg agttctccaa accgacccac
    32521 cgcctgctgg agcagtgcta tgaccgctac tccttcagcg tcctgccgcg cctcggtcgg
    32581 ctggtgaccc aggacagcga gagctaccgc tatctggccg agtcgatccg catgcatccc
    32641 gatcaggagc ggctgcgcgc gatgatggcc gaagccgggc tggccgagtg ccgttaccac
    32701 aacctgaccg gcggcatcgt ggcgctgcac cgggcgatca agccctgagg caccaccgat
    32761 gaccgtgctg cgccgtctgg cactgctgcc actcgaacag agcatcaacc agttgctgca
    32821 ccacgatccg cgcagtctgg cgcggctgga ggcactggcc ggccactgca tcgcggtgcg
    32881 gctgaccacg ccgccgctgc ggctctttct gctgccgcag cacggcgggg tgtcgctgcg
    32941 gctcgaccac cccaccaccg ccgatgtgca gatcgtggcc acgctgccgg cgctggtcgc
    33001 ctacctcggt ggccatgaca gcggtccggc gctgcaactg caaggtgatg ccgagctggc
    33061 cggcacactg caatcgattc ttgcccagct cgacatcgac tgggaggcgg cgctgtcacg
    33121 actgaccggc ggcctgctgg cccatcagac cgggcggctg ctgcgcgcca cccgccggca
    33181 actggcacgc ggcggcgacc acctcaccga catggtcgcc aactggctgg tcgaggagtc
    33241 gcatacgctg ccggcggcag tcgaggtggc gcggttcaac gatgccgtcg atcagctgcg
    33301 gttcgacagc gaccggctcg aagcgcggct gaagcggctc gaacgacaac gcggcgggca
    33361 tggctcggca tgaagaatct gctgcgcctg ctctccatcc tgcgggtgct gttgcgccac
    33421 cggctcgacc tgctgctgcc cgcctcgcac cggccagcgc cgttgcggct gctgctgtgg
    33481 ccgctggcgc tgctgccggc gccggtgcga ccaccgggtg aacggctgcg gctggcctgc
    33541 gagtcgctgg ggccgctctt catcaagttc ggacagatgc tgtcgacccg ccgcgatctg
    33601 ctgcccgagg agatcgccga tgagctgaag aagctgcagg accaggtgcc gccctttgcc
    33661 agcagcgagg cgatggcgct ggtggagcgt gatctcggcc agccgatcga ggcgctgttc
    33721 agcagcttcg acccgacacc gctggcctcg gcctcgatcg cccaggtgca tgccgcccag
    33781 ttgcccgatg gccgcgccgt ctgcgtcaag atcgtccggc ccaccatcga gccggtgatc
    33841 cgccgcgacc tggcgctgct gcaacggctg gccgactttg ccgagcgcaa cttcaacgac
    33901 gcccgccgcc tgcaactgag ccgggtggtg cgcgactatc agaccaccct gctcgatgag
    33961 ctggacctga agcgcgaagc ggccaacacc atgcagttgc gcagcaactt cgctcactcg
    34021 ccgctgctct acgtgcccga ggtccattgg gactacagcc ggccatcgct gctggtgatg
    34081 gagcggatcc atggcattgc ggtctccgag atcgatcagt tgcgcggcgc cggcatcgac
    34141 ctgaagaagc tggccgaacg cggtgtggag atcttcttca cccaggtctt tcgccacaac
    34201 ttctttcatg ccgacatgca ccccggcaac atcttcgtct cgacccggcg gcccgacagc
    34261 ccgcagtaca tcgccatcga ctgcgcgatc atgggcgcgc tgaccgaagc cgaccagcgc
    34321 tatctggcca ccatcctgca cgccttcttc cagcgcgact atcaggaggt ggcccggctg
    34381 cacagcgagt cgggctggat cggcgccgcg gtgccggtgc acgactttgc ggcggcgatc
    34441 cgcacggtct gcgagccgat cttccagaag ccactgaagg aaatctcgct cggcgagctg
    34501 ctgatctacc tgttccagac cgcccgccgc ttcgacatgg agatccagcc gcaactggtg
    34561 ctgctgcaga agaccctgtt gcagatcgaa ggactggggc ggcagctcta ccccgacctc
    34621 gacctctgga gcaccgccag ccccttcatc cagcgctgga tgcaggagcg cagcggaccg
    34681 cgcgcactgg ccggccggct gttgcggcag ttgccgaact gggccgagca actgccggag
    34741 ctgccgaggc ggctgcagca gagctggttg cagatcgatc agcttggtca actgcaacgg
    34801 gcgcaagcgg cggcactgca gcagctccag cagggcatcg aacggcggca gcggcggcaa
    34861 cggctgatcg cattgctgct gctggtttcg ggtctcggct ggctcttcag cagccaccac
    34921 gacccgcagg gcagcctcag cggcggcggg ctgctcagcc tgctggccgg actgtggctc
    34981 tacgcggcaa aatgaaccgc tctcattccg gaggcagtcg accatgagcg atgtcctgca
    35041 acggctggcc gaggtgctgg aggcacgcaa gacgatggcc gcggccgact cctatgtcgc
    35101 cagcctgcat gccaaggggc tcgatcagat tctgaagaag gtgggcgaag aggcggtcga
    35161 gaccgtgctg gcggccaagc agatgcagtc gagcggcgac ggcgagccgc tgatccgcga
    35221 gaccgccgac ctctggttcc actgcctggt gatgctgagc cacctcggcc tgcaccacga
    35281 ccaggtgctg gcggagctgc aacggcgctt tggcctgtcg ggcctggccg agaaggccgc
    35341 ccggcccaca tccaatcaca gctaggagga gtcgacatgg gacgctttgg tctcacggag
    35401 ttactgctga tcttcggcat cgtgctgctg ctgttcggca ccaagcggct gcgcaacctg
    35461 ggcggcgacc tcggcaatgc gctgaagggg tttcgcgatg ccatgaagga tgacaaaggc
    35521 ggcgacaagg tcgaagagca gccgagtgca cgggtgatcg aaggcgaagc caaagagaag
    35581 agcccggaac gtcagccgta gcggcgggag ttcgccatgt tcgacatcgg tttcagcgaa
    35641 ctgctgctgg tgggggtcgt cgccctgctg gtgttcggcc cggagcggct gcccgaactg
    35701 gcgcgcaacg gcgcactctg gctcggccgg ctgcgccgcg ccatgcgcga tgccaagcag
    35761 agcatcaagg atgagctggg caccgacgag atcatgcagc aactgcacaa cgaagaggtg
    35821 ctgcgcaagc tgggcgagga tcccaaccgc ctgagcaggc ccgcaccacc gccagcgcca
    35881 cccgtcacca ccgcgcatga cccggccaat gagcgccact gacccacaag cggatgatgc
    35941 agccaccgaa tcgtcgctgc tgttccacct gcgcgagctg cgcagccgac tgatccgcat
    36001 cgtggcggtg acggtgctgc tgctgttggc gctgctgccg tttgccaacg agatctacct
    36061 gttcttctcc aggccgttga ccgcactgct gccagccggc agcagcatga tcgccaccga
    36121 ggtggcctcg cccttcttcg ccccgttcaa gctgacctcg gtggtggcgc tgctgctggc
    36181 gatgccctat ctgctctacc agctctggag cttcatcgcc ccgggcatgt atcgccacga
    36241 aaaacgcttt gcgctgccgc tgctgctggc cagcatcctg ctgttctatg gcggcatcgc
    36301 ctttggttat tttctggtgc tgccgacgct gttcgccttc ttcaccagca cggcgccgca
    36361 aggggtcgcg ctgatgaccg acatcaacca gtatctcgac ttcgtgctga agtttctgtt
    36421 ctcgttcggc atcaccttcg aggtgcccgt cgccaccgtg ctgctgatcc gcgccggcat
    36481 cgtgtcggcc gccgccctgg ccgacaagcg tccccatgtg atcgtgctct gcttcgtcat
    36541 cggcatggtg ctgacgccac ccgatgtctt ctcgcagacg atgctggcag tgccgatgtg
    36601 gctgctgttc gaggccggac tgctgtgcgg acggctgctg acgccgccgg cggagccggt
    36661 gaagacggag gtcacgacgc aaccgccgca ctgaaacccc gtcccggccg gtcgcgggcg
    36721 tcagctttcc agccagaagg tgaccggacc ctgattgagc aggctgaccg ccatgtcggc
    36781 gccgtagcgt cctgacgcca ccttgcaggg gagctgcgct gcctgcgcca gcagcaggtc
    36841 gaacagctcg cgcccctgcg ccgggctggc ggcggatgag aagctgggtc gcaatccccg
    36901 gcgggtgtcg gccaccagcg tgaactgcgg caccagcagc agctcacctc cggtgtcgag
    36961 cagactgcgg ttcatccggc cactctcatc ggcaaagatg cggtagcgca ccagccgatc
    37021 gagcagaagc gctgcccgcg ccggcgtgtc gtccggctcg acgccgatca gcgcgaggat
    37081 gcccgcaccg atcgcgccga cctcttcacc cgccaccacc accctggccc aggcgacccg
    37141 ctggatcact gccttcatgc ggtcggcaat gtgccatcgc tgaaccggcg cgccatctcg
    37201 tcggtggcgc gaatcagcgc atcggtgatg cccggttcgg tggcggcgtg gccggcctcg
    37261 cggatgatct gcaactcggc gtcgggccag tggttctgca gggcgatggc gttgtcgagc
    37321 ggacagacca tgtcgtagcg gccatggatc agcatgccgg gaatgccggc cagccgttcg
    37381 gcgttgcgca gaatctggtt ggcttcgaga aaggtctcat gggcgaagta gtgcgcctcg
    37441 atgcgcgcca gggccagcgc ccgctgtggt ttgatgaacc agtcgatcag atccttgcgc
    37501 ggcttcagcg tcgagcagcg cgcctcccac tgtgcccaca ccttggcagc gcccatgcgg
    37561 gcgatctcgt cgctgccggt cagccggtgg tagtaggcgg tgatcatcgc atcgcgctcc
    37621 gccggcggaa tcggttcgag gtagtcgcgc cagtaatcgg gaaagaccag gctcgccccc
    37681 tgctgataga accagtgcag atcctgctgg cgacagagaa agatgccacg cagcaccatc
    37741 gccagcaccc gttcgggatg gctctgggca tagaccaggc tgagggtcga gccccaggag
    37801 ccgccgaaca gcagccagcg ctcgatgccg agcagtgtgc ggatgcgctc catgtcaccg
    37861 accagatggt gggtggtgtt ctctttcagt tcggcatgcg gggtcgagcg gccgcagccg
    37921 cgctgatcga agcagatgat gcggtacttc tcggggttga agaagcggcg gctgttggca
    37981 tcgcaggcgg cgcccggtcc accatggacg aagagcaccg gaatgccccg tggattgcca
    38041 ctctcctcca gatagagctc atgcaggtcg ctgaccttca gccagtggcg ttgatagggt
    38101 ttgatttccg gatagagcac ctgcatcacg taagcccgct gctgtgtgtg gatgaaaaag
    38161 agcgtggcca gccgccagga ctctcccgct ctggcgccga tcaggcagag atggtaacgg
    38221 attgattcgc ccgagggtga atccgttgcg gccggatcga ccgccccatc tctgcctgca
    38281 tctggcaaaa tctcgactat gcttgaccca tgaggctctt caccgatgcc cgaccctctg
    38341 ccattgccga cggttggcca ctgacgcatc acgccaaaga attccgcctt gcaaaatcca
    38401 gaccccgaaa acgataaaga gaggaatggg gaagccgtgc ttcatccgct gctggccgaa
    38461 ttcatctcgg accggctgga gcggcgcttt ctgcgcacac tctggccgca ccaacgcgat
    38521 cagatttatc tgctctccct gatcggcggt ctgctgttct cgatcgcagt ctatggtgac
    38581 tatctgcgct ttggcgcagc cactctctcg acccccatcg ggctggcgcg gttcggtctg
    38641 attctgctct gcgccctgcc ggccgtggcg gccgcctgcg acagagcacg cctggccaac
    38701 cagctctgct ttctcagcat ggccggcatt ggcgggctct acctctatct ctgccacgcg
    38761 caggccctgg atccacaggc gcgccttggc acgctgtttc tgatgacgac cctgttctat
    38821 ctctgcgcac cactgcgtct gctgtggacc atcaccttca acctgctgtt gagtctgggt
    38881 tttctgtggg tgaacagcct gacgcccggg atcgccccct atga
//
LOCUS       DAORCQ010000011        38502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_526_length_38502_cov_5.804432, whole genome shotgun sequence.
ACCESSION   DAORCQ010000011 DAORCQ010000000
VERSION     DAORCQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 38502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38502
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_526_length_38502_cov_5.804432"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..700
                     /locus_tag="PKI23_02005"
     CDS             17..700
                     /locus_tag="PKI23_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010566349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNN35743.1"
                     /translation="MKAMILAAGKGTRMRPLTLHTPKALLPVGGKPLILHHIEALRRS
                     GVRDIVINCAHLGQQIIDALGDGQRHGVRLHYSQEPDEPLETGGGILQALPLLGDAPF
                     LIVNGDVLTDFDFARLRLPPGKLAHLLLVENPDHHREGDFYLSPSAAVRSQAMPGARR
                     LTYSGIALLSPQLFAGCHPGVFPLAPLLIAAMQNDRVSGELHPGRWLDVGTPERLQRA
                     ERELAGGVA"
     gene            697..1530
                     /gene="djlA"
                     /locus_tag="PKI23_02010"
     CDS             697..1530
                     /gene="djlA"
                     /locus_tag="PKI23_02010"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HNN35744.1"
                     /translation="MKRFSIVASQPPWIGKLLGALTGLIWAPDSLHWSGLVLGLLAGH
                     LLDQRLGGLARADTSAQKTVAALRPLLMLMGHLAKIDGRVNEAEIAVTSRLIDRMVTQ
                     GRDRAELIELFRQGKGATPAEIDRQLDRLADTLCHQPKLAEQWLGMLIGLALSDGALC
                     AGERALIERIAVRIEIAPETLRRIESRLRRQQSRPAEPATTPVSVLMQAYQLLQVNRN
                     ASDEEVKLAYRRKLSQHHPDRLQAGGASAEQIQAAGQQTHEIRSAYELIRAARLFRQS
                     E"
     gene            complement(1514..1957)
                     /locus_tag="PKI23_02015"
     CDS             complement(1514..1957)
                     /locus_tag="PKI23_02015"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HNN35745.1"
                     /translation="MTTTPFEERRRNRPWINLRHLGIAGRRRTAPQPRLARNRYVDLY
                     EPVYLLIAVLLIIAAAGDALMTLRLIEHGAQELNWLMAFWLVNDPDYFVQSKLLITAL
                     SVIFLTIHKNFVLFGRIPVSTLLRTACGGYLTLLGYEAALLTLIA"
     gene            2128..2808
                     /gene="rpe"
                     /locus_tag="PKI23_02020"
     CDS             2128..2808
                     /gene="rpe"
                     /locus_tag="PKI23_02020"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNN35746.1"
                     /translation="MEFLLAPSILSADLARLGDDVAAMLAAGTDWVHFDVMDNHYVPN
                     LTFGPSVVKALRGYGITAPIDVHLMVKPVDRIIGDFIEAGASLITFHPEASEHIDRSL
                     QLIIDGGCAAGLVFNPATPLNHLDHLLDKLSLVLLMSVNPGFGGQKFIPGTLAKIEQA
                     RRRLDAAPNPVRLEVDGGIGLHNIRAVAEAGADTFVAGSALFGSDDYRATVAAMRAEL
                     ARIERNPA"
     gene            2866..3672
                     /locus_tag="PKI23_02025"
     CDS             2866..3672
                     /locus_tag="PKI23_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNN35747.1"
                     /translation="MIQVRPDPKIDADEPTLRLAVLIDADNAQAAVIEALLAEIARFG
                     EATVKRIYGDFTSPTSASWKKVLQRYAIKPVQQFAYTTGKNATDSTLIIDAMDLLYTR
                     KFDGFCLITSDSDFTGLAMRLREEGLTVLGFGEKKTPEAFRNACHKFIFTEVLRPAMT
                     AKPAAAATRTARRKKSAPAQTLAETNEPKLEFPTEFVLTALDQSIDDAGWAQVGTFGS
                     YLTKLQPDFDARLYGYRKLSDLVRARTDLFVTEERQAAGSSQKVLYLRAK"
     gene            3852..5336
                     /gene="trpE"
                     /locus_tag="PKI23_02030"
     CDS             3852..5336
                     /gene="trpE"
                     /locus_tag="PKI23_02030"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNN35748.1"
                     /translation="MTETEFNRLAAEGYNRLPLTLETFADLDTPLSIYLKLADAPNSF
                     LLESVVGGERFGRYSFIGLPAHTLLRVRESITEVVSHGAVIERHEGNPLDFLEQYQAR
                     CKVHVPVGFPRFCGGLAGYFGYDTIRYIESKLAGFDHPDEIGMPDILLLQSEELAVID
                     NLASKIYLIVYVDPALPAAYAQGQARLQQLLELLRGPLTLPQSRPSPATLPQRDFRKE
                     DYLAAVLRAKEYIAAGDMMQVQIGQRIKQPFSDSPLTLYRALRSLNPSPYMYYYDFGD
                     FQVVGASPEILVRQESSGSGTTKVTIRPLAGTRPRGADPQQDRHNAEELLSDPKERAE
                     HVMLIDLARNDIGRIAETGSVKVTDQMVIERYSHVMHIVSNVEGVLKPGTSNLDVLRA
                     TFPAGTLTGAPKVRAMEIIDELEPIKRGLYGGACGYLSFSGEMDLAIAIRTGIVKQGM
                     LYVQAAAGIVADSVPESEWQETENKARALLRAAEQVQAGLDHVP"
     gene            5350..5805
                     /locus_tag="PKI23_02035"
     CDS             5350..5805
                     /locus_tag="PKI23_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNN35749.1"
                     /translation="MAKLLILFHSQSGNTLQLAQSVYRGALAVEEVESRLLRATDAGL
                     DELLWCDGLLLGTPENFGYMSGGLKDFFDRTYYPAQDRVTAKPYALFISCENDGTGAV
                     GQIERIARGYALKPVLEPLICRGAVSASCLAQAHELGMTMAAGLDIGIY"
     gene            complement(5904..6326)
                     /locus_tag="PKI23_02040"
     CDS             complement(5904..6326)
                     /locus_tag="PKI23_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNN35750.1"
                     /translation="MADSAFDKIKEGDEYSFVVKNFNRTQFVKYAGAGGDFNPIHHDQ
                     TFAESAGLPTVFGMGMLTSGMLARVLTDWFGATSVKKYGFRFKTRLWPGDDVTFKGVA
                     TKVYSEGGVKHVDLDLTAVNQKGEVLIESNATCRPWKP"
     gene            complement(6319..6843)
                     /locus_tag="PKI23_02045"
     CDS             complement(6319..6843)
                     /locus_tag="PKI23_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNN35751.1"
                     /translation="MRNNSTSPPGDAITTASVKQGERTLMATINPPGFPIERGKIHEF
                     ANSLQDDNPMYHDEVAAKAAGLPSVPAPPTYTATSAHFSSGDDGSAIKSLGLDLRFVL
                     HGGQEFEFVRPLFAGDVLTMRTGEVKSFVKEGKRGGQMKIFDMFSEYVDQNGNVVIKV
                     KNTVLQTGGVVKDG"
     gene            complement(6968..8251)
                     /locus_tag="PKI23_02050"
     CDS             complement(6968..8251)
                     /locus_tag="PKI23_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN35752.1"
                     /translation="MQPLPENPADPSAKPPFHGWRMVAIALFTDFISVGFFFYSYGIF
                     FLALSKEFGGSHLDVSLGFMISNVSGALMAPFLGRALDRYPTRWIMLLGATVLALGYG
                     LLSTISALWQFYLILATCNAFGGAAMGGQASSKLVAAWFIRKRGLALGIATMGISASG
                     LVMPTLSAWLIDHIGWRGGYLVYCTVALLCVTPVVALLVRSNPEELGQRPDGATDAQH
                     RTLLGGAEVEWSTAAILRSSNFWFIALTFALLVGVFQAILIHMVPQLTSQGLSTTQAA
                     MILSVSAGIGVIGKVVFGWLTDHNEPKLAVWLCIITQLLGAILLMLCSSYLWLLLGGA
                     LFGFGMGGVVPLQSSTTGQIFGRLSFGRVSGLIRPAMIPLQMCAVPLAGWIYDQTGSY
                     RISYLIMILALLIAAITIALVKVPAREPDVAAAIE"
     gene            complement(8374..9657)
                     /locus_tag="PKI23_02055"
     CDS             complement(8374..9657)
                     /locus_tag="PKI23_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN35753.1"
                     /translation="MRKKNDGMFYGWVLLAVISLCYFLGVGLCMYGLSVVLPLMIEEF
                     GWSRGQASVGFSLLLLVWGVSAPLVSIFIRRFSTRKTYLTGGILIVCGALLIYQTSSL
                     YIFYLGTGFFIAIGLSMTSVLPGSNLLVTWFVQRRALAMGLFMANGGLGGFVASPMVA
                     ALVEQTGNWRIVWLLMAVCGLLVGLLGFWAIRERPEEIGQHPDGVKNPAEAHVTATST
                     NAVFKTSENWTLREALRTRFFWLTVLVACAATTASATINAHLILHLSDIGVSSLVAAS
                     SLGMVAILNSTGRILSGGIGDRIDPRWILAAGMVFQAIGFLLLNFATSQPLVYLFTVL
                     FGMGIGVYLIALTALTANYFGALNFSAIAAIQALSYTLVAASGPIAAGAIFDRTGSYQ
                     TVFISLAALTALCALCVGWMKPPVRSTPSIEAAGN"
     gene            complement(9848..10033)
                     /locus_tag="PKI23_02060"
     CDS             complement(9848..10033)
                     /locus_tag="PKI23_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNN35754.1"
                     /translation="MDPKLLQILVCPVTKAPLVYHKERNELLCKASGLAYPIRDDIPI
                     MLESEARLLTEEEKKQL"
     gene            10190..10744
                     /gene="orn"
                     /locus_tag="PKI23_02065"
     CDS             10190..10744
                     /gene="orn"
                     /locus_tag="PKI23_02065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNN35755.1"
                     /translation="MSRADHLIWIDLEMTGLDPDRDRIIEIATLITDADLNVVAEGPV
                     MAIHQSDEVLAAMDEWNQHHHGVSGLIDRVRTSGVDEQDAERITLDFLGRYIEPRRSP
                     MCGNSICQDRRFLARHMPRLERFFHYRNLDVSTIKELARRWRPELLNGLNKRATHVAM
                     DDVKESIAELSHYRAQFFRMNEDG"
     gene            10930..11439
                     /locus_tag="PKI23_02070"
     CDS             10930..11439
                     /locus_tag="PKI23_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNN35756.1"
                     /translation="MSTITAPPAMTRWLAGSRWAPDFVSGGADPALFCDCCAAGSGVP
                     SLTALPAALQEARRAETLSLIRAQLRHFALLLYMKGKPDQPRCARSARVVALLRHAQL
                     PFAFVDVLAQPLIDELLQEFSGLSGYPQLFLDGNLVGGYAAIARAIEDGTLSRWIEQR
                     CRAEEVVIP"
     gene            11436..11729
                     /locus_tag="PKI23_02075"
     CDS             11436..11729
                     /locus_tag="PKI23_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35757.1"
                     /translation="MTPQQIEHCIIEDLPDAVIDVEIKRGATVLRVVSQKFAGRGQSE
                     RQRHVLSLLKQGLANGSLHISHLKLYSHDEWAVLKPTEEILLIQRNGSWPASS"
     gene            11780..13177
                     /locus_tag="PKI23_02080"
     CDS             11780..13177
                     /locus_tag="PKI23_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNN35758.1"
                     /translation="MLTALLRRKSISQALALSEENGLHRALGRFDLIFLGIGAVIGAG
                     IFVLTGIAAATKAGPALSLSFVVAGLACFFAALVYAELASTVPVSGSAYTYSYVTMGE
                     FIAWIIGWDLILEYGVASAAVAIGWSGYFLQLSTAMGLDLPELLTKAPHEGGWINLPA
                     AGVIMLVTLLLAAGVRQSSRVNNVVVLVKLAVIGLFLVVAAPKVQVGNWQPFFPFGWQ
                     GVMGGAALIFFAYIGFDAVSTAAEEARNPQRDLPLGILGSLLFCTVIYIAVAMVLTGA
                     VHYGQLNVSHPVAHALELIGSPTAAGLVSVGAIAGLSSVLLVLLYGQSRIFFAMSRDG
                     LLPVAFSQVHPRTRTPVTTIIATGVVVALVAGLLPIGAVAELVNVGTLAAFMLVSIGV
                     IVLRRTQPELKRPFRTPFMPWTPILAILFCGYLIASLDAVTLLRFVVWMAIGMVLYFC
                     YSVRHSTLGRAAAND"
     gene            complement(13193..15121)
                     /locus_tag="PKI23_02085"
     CDS             complement(13193..15121)
                     /locus_tag="PKI23_02085"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNN35759.1"
                     /translation="MAEPNTLLELPAGKLQERVDFLEELNRWYANELDILSSIAVVYQ
                     HCLPHGNSERILGATLPFLQRIAPFNAIGFALTDEEGLNFNLRGVGSGSFDVTLPRLL
                     DQLIDNGQFGWAVKQQLPTLYKQKEQVNVLACPIAVPNQTLGMMIATFEDERALPPRA
                     QRLLMAVLQHCAYALHNVRLYHQVERQNHYLEKLITERNDQLEHASTYDALTDLPTRE
                     HFQEEVRREIQRAQRDGSSMALILLDIDFFKRINESLGHAVGDLILKRVAQRLASNVR
                     EYDPLSRLVQSSDISHVGRFGGDEFCVLLSGQNAQSQINAIIERISRALSEPFKVAQH
                     ELLLAFSAGVACYPDHAAEPEKLLQLAEIALHQAKQRGRNRHLLYQPSMGQESLHQLA
                     LGNKLRHALVNGQFQLHYQPQIDIREHQVIGIEALLRWQDESGDWIPPNHFIPLAEES
                     GMITPIGAWVIEEVCRLQSQLQKQGYSIPVAINISAHQFTHATFLDSLLETMRRHQVP
                     AELIELELTERAIMQDVQETIDRLQAIHRQGIKISIDDFGTGYSSLSYLKRFPIDILK
                     IDRSFIQDVDSNPEDAAIVNTIIALGKSLNLQLIAEGVERAEQVRFLREQGCYGIQGY
                     FYSRPLSEADLLRYLELG"
     gene            complement(15114..15974)
                     /locus_tag="PKI23_02090"
     CDS             complement(15114..15974)
                     /locus_tag="PKI23_02090"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNN35760.1"
                     /translation="MAPLSAESIAANTTTVASLPELHQQLEAAILSPASTLDDLARII
                     GNEPGLAARLLKLANSALYRHSGPIETITRALTLIGTRQLQELALATLVIERFRLLET
                     PQLSMREFWRNALATAIAARVIAIHLRERDSERFFLAGLFHKIGRLVLFTDHPELATQ
                     SLIQAQQAGLLLSDAEEQLLGFHHAEIGHALLKQWNLPALHCEGVRHHIAPSIAPTTS
                     VVACIVHLANWLADGAAFGHAGDHFAPRLDPGALRTLGLCAATLESLLNELERQYQDV
                     EPLLLGETDG"
     gene            complement(15955..16302)
                     /locus_tag="PKI23_02095"
     CDS             complement(15955..16302)
                     /locus_tag="PKI23_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35761.1"
                     /translation="MQSLSIQHAQPGMRIIHDIKDGNGRILLPADCTLDERKLKALRS
                     WGITEVVVDGAEALRPGKVELPEAQSLLVEAIVCERFRHNDPADPAVEEMKRLVFNRV
                     AKQLGYLHGTAQR"
     gene            16532..18649
                     /gene="flhA"
                     /locus_tag="PKI23_02100"
     CDS             16532..18649
                     /gene="flhA"
                     /locus_tag="PKI23_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNN35762.1"
                     /translation="MNTRLDAGPAPIAWRRLLQGSLGIPLILLALLGMMVLPLPAALL
                     DLFFTFNITLSIAVLLVCVYARKPLDFAVFPTVLLITTLFRLALNVASTRVVLLHGHE
                     GGDAAGKVIEAFGEVVIGGNYAVGLVVFAILMIINFAVVTKGAGRISEVSARFTLDAM
                     PGKQMAIDADLNAGMLTQDEAQQRRREVAQEADFYGAMDGASKFIRGDAIAGLLILVI
                     NLLGGLGIGMLQHDLGFAEAMSRYSLLTIGDGLVAQLPSLMLSIAAAIMVTRVESMSD
                     MSEQIVAQMFSSPRILLVTAAVMGVIGAIPGMPHLIFGVLAAATGALGYWLEQKKQRA
                     LLAPVTDEAPVVAVTPEVRELGWDDVTPVDVTGLEVGYRLIPLVDSTQGGELLARIKG
                     IRKKLSQDLGFLIPSVHIRDNLELPPNHYRIILMGVAVAEGRIEMGRELAINPGKVFG
                     TLPGIETQDPAFGLPAVWIDPGQRERAQSLGYTVVDGSTVVATHLNQILLEHAVDLLG
                     HEEVQKMVDLLIKSSPKLAEELIPNRISLSLLLKILQNLLRERVSIRDLRTIAEAITS
                     VTPPSQDPTVLTAAVRIALSRMIVQGLVGNGGEIPVITLEPQLEQMLLSSYKQARDAG
                     NPPEEGMVIEPSLAERMQRMLREAAARQEMDGQSAVLLVSAPLRPVLARFVRYGGVSM
                     SVLSYQEVPENKQIMVVATVGRH"
     gene            18677..20176
                     /gene="flhF"
                     /locus_tag="PKI23_02105"
     CDS             18677..20176
                     /gene="flhF"
                     /locus_tag="PKI23_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNN35763.1"
                     /translation="MQIRRFVAPDSRTALQQAREALGPDAVILANRTLANGVEILCTA
                     DYPLPMQPQGDAPLAAPTMAAAPVQQPAEPATGTATAMASFVPASATTDTAQGTASAA
                     VGQSRVPSAIRFPRTGQFRLGEKVASMLTKEFQQARPQNQQPPTQQPIATPKAAELPK
                     GAAENGAHAAVSTQWAERHELEQLRNELQGVRELLSRRIVAPAAAPLHAQGESIPPSP
                     WRRTLEERLQRLGLSARLAARLLDRFDADVSAESELVWREVLGELVARIPVVGEDRAM
                     QGGVIALIGPTGAGKTTTVGKLAARHVLRHGADQVALVTTDCHRVAAHEQLRIFGRIL
                     DIPVKVVDERRDLDTVLHSLRDRRLILIDTAGLHPASEQFASQLATLSSARTPIDSYL
                     LLPCTSQRSVLERTVRHYGAIDLAGVMLTKLDEACSLGEALEVVIERQFSIAYVTDGQ
                     SIPDDIRLPSALELVSQAVDLMETEQNGMKQDLIWASPASQESTLAVPA"
     gene            20223..21050
                     /locus_tag="PKI23_02110"
     CDS             20223..21050
                     /locus_tag="PKI23_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNN35764.1"
                     /translation="MNSFHPVQVIAVTGGKGGVGKTNVSVNLSLALADLGRRVALLDA
                     DLGLANVDILLGLTPKKNLANVINGECGLKEVMLDGPNGIRIFPASSGTQKMVGLDPR
                     EHLGLIHAFSELDDELDLMVIDTAAGISDSVVSFVRASQEVLVVVCDEPTSIADAYGL
                     IKVLNRDHGLSRFRILPNMVKSPQEASQIFAKLTKATDRFLDASLQLCGFIPYDDFLR
                     KAVKRQRALYELYPGAMASKAFQELAKKVIAWPVPKTPSGHLQFFVERLLAPLRATG"
     gene            21054..21788
                     /locus_tag="PKI23_02115"
     CDS             21054..21788
                     /locus_tag="PKI23_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNN35765.1"
                     /translation="MRHTLYAEASRCTTSELVTRHSELVKRIAYHLLCRLPPTIQIED
                     LVQAGMVALIEAAQKFDAGKGASFETYAGIRIRGAMIDEVRKGDWTPRSVHRNARAIS
                     EAIQLLENRLGREARESEIAAELGVDLASYHTMLQDQAFAKLQSFEELLPEAEEDEGD
                     EFDLPGSHAGPQQQIEREHFIAALGSAIGQLPQREQLVLSLYYDEELNLKEIAAVLGV
                     TESRISQIISQAALRLRARLSSWRSS"
     gene            21978..22364
                     /gene="cheY"
                     /locus_tag="PKI23_02120"
     CDS             21978..22364
                     /gene="cheY"
                     /locus_tag="PKI23_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNN35766.1"
                     /translation="MDKNMKILIVDDFSTMRRIIKNLLRDLGFTNTDEADDGLTGLPM
                     LQKGNYDFLVTDWNMPGMTGLDLLKAVRADERLKNLPILMVTAEAKREQIIEAAQAGV
                     SGYVVKPFTAATLKEKIEKIFERIAA"
     gene            22389..23174
                     /locus_tag="PKI23_02125"
     CDS             22389..23174
                     /locus_tag="PKI23_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNN35767.1"
                     /translation="MIQMEQHIERDETMEHLVDKARSLVENLESGNLYDTHKLLGDIY
                     AARERKLYEEVGKLTRGLHDALMDFHIDAGIGEEAQEEISKISDASDRLGYVIKMTDD
                     AANLTMDKVDEAVPIADEIRSTSKDLNEDWKRFKRRELTPEEFRNLYQRVGDYLDGTE
                     VRAERMQNSLNDILMAQGYQDLTGQVIKRVITLVKEVENSLVELVRMASSVEKIAGIN
                     AETGDSVEPQEVALAGPNVHGEKNVTVVSGQDEVDDLLSSLGF"
     gene            23186..25237
                     /locus_tag="PKI23_02130"
     CDS             23186..25237
                     /locus_tag="PKI23_02130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017683751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNN35768.1"
                     /translation="MNHEDEEILQDFLLEAGEILEQLSEQLVALERDADNIDLLNSIF
                     RGFHTVKGGAGFLGLKPLVEICHVTENLFDALRKGQCRITGELMDLTLQGLDSINVMY
                     QQLQQREELAAANSGLLARIKRLVEGEQECVAPKKEVETARPAAAVPQKKVAGCANDV
                     VEQALREMLDEAEAPAAQGLSAAERDEITDDEFEMLLDQLHGKGKHGGVPANKTDGVK
                     IDNKAAGSDLINDDEFEAVLDGLYGKGKGPTFEQKSGKTRHNDVVSTPVSALESSSSS
                     GILKKAEPVSAASAKETPAAAAENTVRVDTHRLDDIMNMVGELVLVRNRLVRLGVDSH
                     DEELSKVVTHLDVVTADLQNAVMKTRMQPIKKVFGRFPRVVRDLARSLGKEINLEMIG
                     EETDLDKNLVEALADPLIHMVRNSVDHGIEMPDERVESGKSRAGRIVLSAVQEGDHII
                     LSITDDGRGMDPNKLRAIAVARGVLDKDAAERLSDTECFDLIFAPGFSTKSEISDISG
                     RGVGMDVVKTRIGQLNGSVFITSELGRGTKIDIKVPLTLAIMPTLMVMLGDQAFAFPL
                     ANVNEIFMLDLSDTNVVNGQEIILVRNKPLPLFHLGRWLCKNPGETRNVENAHVVIVT
                     VGNQRIGLVVDQLLGQEEVVIKSLGAMLAGTQGVAGATITGDGRIALILDIPGILKKY
                     A"
     gene            25347..26393
                     /locus_tag="PKI23_02135"
     CDS             25347..26393
                     /locus_tag="PKI23_02135"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNN35769.1"
                     /translation="MSIRVLVVDDSRFFCRRLCEILSSDPEIEVVDTAMNGQEAIDKT
                     IEFSPDLVTMDYAMPMMDGIQAVRGIMERRPTPILMLSSMTSEGTQVTLDSLEAGAVD
                     FIAKDSIALAGRDGAQRADLISRVKGLASSNFRARRTALAHGVGTRSARPVTARSATK
                     RIRLVVIGASTGGPVAVQKILQELPATFSAPIVVAIHMPQTFTGIFAERLNQSSKLKV
                     KEAAEGDLLRSGEVLLAPGNKILSISGQQGGCIHLNEPGRNDIYSPSVDHAFSTAAQA
                     FPGSVLGVVLTGMGQDGLLGAKQLKESHSRLWAQDQASSVIYGMPMAVSKANLVDHVY
                     SLAELSEHLVAEVC"
     gene            26393..27133
                     /locus_tag="PKI23_02140"
     CDS             26393..27133
                     /locus_tag="PKI23_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNN35770.1"
                     /translation="MDILSILGLIVGFGGIIGGNLLEGGHISALLNLPAIIIVASGAF
                     GAVMLQTPMPTFLRGLKMMAWVFMPPPVRIDEAIQKIIEWSQIARKEGLLGLEAIAED
                     EPHPFAKKGLQLVVDGGEPDDIREILEVELHQIEVRDLASAKIYEGLGGYTPTVGIIG
                     AVLGLIHVMGNLADPSALGPGIAVAFVATIYGVALANLIFLPVGNKLKSVVQSQSRYY
                     EVMIEGLLSIAEGENPRIIELKLSGYLS"
     gene            27142..28062
                     /gene="motD"
                     /locus_tag="PKI23_02145"
     CDS             27142..28062
                     /gene="motD"
                     /locus_tag="PKI23_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNN35771.1"
                     /translation="MAKRHVEHEHENHERWLVSYADFITLLFAFFVVMYAVSSVNEGK
                     MKVLSEAMTEEFRTRSVQFPVPPTSLKPIQIGELNREQPASMVEGHKLSPGNQIEQAQ
                     GDEAEEELEGIKKELEVKLGELIGENLLTISGNGRWLEIEINSNILFESGEAEPIGEA
                     NTIFLKLAQILGEYGNAIRVEGFTDDLPINTARFPSNWELSAARAARIVTILTKYGVD
                     PRRMAAVGYGEYQPVGDNRSSEGRARNRRVVLVISADRQVRKQLDLSVDSPGQSRDVM
                     KLDGSEKSETVQRPEVIGRNPAVSASGNSN"
     gene            28103..28903
                     /locus_tag="PKI23_02150"
     CDS             28103..28903
                     /locus_tag="PKI23_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNN35772.1"
                     /translation="MEIIASANQKGGVGKTTSVVTLAGLLAKNGSRVLMVDIDPQCSL
                     TCYFGFTPELLDATSYHLFPVPGGNIDAVDMLIPLEEKNLVLLPSSPAIATVEKQLFD
                     QKGMGFVISSALKGLRSHFDYIFIDTPPALGILMINALAACDKLLVPVQTEYLALKGL
                     ERMIGSLEMMARIRKKPLDYTIVPTMVDRRTQASLRSLQALRDNYRRNIWSGSIPVDT
                     KFREASLLGRPLANAFPSSRGAKAYEALWHDLTRTHPSHVESDALEMA"
     gene            28989..29468
                     /locus_tag="PKI23_02155"
     CDS             28989..29468
                     /locus_tag="PKI23_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNN35773.1"
                     /translation="MNTAAAQNAYDQVIQWVSFKLDGETYCIDVMKVQEVLRYTEIAP
                     VPGAPSYVLGIINLRGKIVTVIDTRARFGLKEDEITSNTRIVIVETSQKIIGILVDSV
                     SEVVYIRQSEIETAPNVGSMDNAKFIQGVCSKNDELYILVDLDQLLNEDEWLEVEKV"
     gene            29517..29921
                     /locus_tag="PKI23_02160"
     CDS             29517..29921
                     /locus_tag="PKI23_02160"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNN35774.1"
                     /translation="MTDLSALEQSWPALLLFFSTLLASCLLARMQFNRFEKKITSDLK
                     VLAQTVALIEQSSFGFGRNLHLIERNVKELRMLHDELESKSGAEDLYLQAAQVAEMGA
                     TAAELMNNFGLSKVEAKLIEALHAARADARLH"
     gene            complement(30137..31516)
                     /gene="tilS"
                     /locus_tag="PKI23_02165"
     CDS             complement(30137..31516)
                     /gene="tilS"
                     /locus_tag="PKI23_02165"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNN35775.1"
                     /translation="MSDSPVVTALSRQLAAEPAPARWVVAFSGGLDSMVLLHATVHCL
                     AQRRLHALPTQPLHALHVQHDLHPEAATWASHCRALAQQLEVPFHLRCVAVDRSGGRG
                     VEAAAREVRYRAFSDFLLPGDQLLMAHHQDDQIETLLLRLLRGSGPAGVAGIPAQRPL
                     GHASLARPLLALPRTQLRAYADAVQLTWIEDPSNLDPAFDRNYLRHTLRPAIAARWPN
                     HARTLLRSAALAAESAQLLQQLAAIDFSNAPQHRLDRLHIPSLQQLDDARQRNLLRHW
                     LGLNGLPLPDHNRLQRILDEVMPAAADRQPLVNWPGVEVRRYRDFLYAMPPLPPPAST
                     MELEWDLSTAFLQLPDGSRLWQQWQDAGGVALRPGIPITLRFRHSGDPAQQADRHRLK
                     RLLQQAGVPPWLRSHIPLIYQDHSLVAIPGVAIMPGWRSAAGAGGLHFRWQPAGSHVL
                     PAFADSGGP"
     gene            complement(31529..32485)
                     /locus_tag="PKI23_02170"
     CDS             complement(31529..32485)
                     /locus_tag="PKI23_02170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNN35776.1"
                     /translation="MHLNHLDFEQPIAELRAKIEELRMVGSDNELNISDEIAKLEEKS
                     RKLTESIFGNLSAWQIVQISRHPLRPYALDYINRIFDEFDELHGDRHFRDDPSIIGGL
                     ARLDGEPVMVIAHEKGRGTKEKILRNFGMPQPEGYRKACRLMELAERFSIPIITLIDT
                     PGAYPGIESEERGQSEAIARNLAVMSRLKTPILATVIGEGGSGGALALGVCDHLMMLQ
                     YATYSVISPEGCSTILWKSNEKAAEAAELMAMIAPRLKELGLVDQVIPEPLGGAHRDY
                     DATAAAMKHALIERLAEFKRHSAEELVERRYRRYLSYGHYVN"
     gene            complement(32552..36019)
                     /gene="dnaE"
                     /locus_tag="PKI23_02175"
     CDS             complement(32552..36019)
                     /gene="dnaE"
                     /locus_tag="PKI23_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003119349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNN35777.1"
                     /translation="MNSTAPGFVHLHLHSEYSLVDGLITIDALVERTRELAMPAVAIT
                     DISNLFALVKFYRAARSAGIKPIVGAQLLVENPEQPGQFAPLLLLVRDQTGYRNLTTL
                     ISRGYLENRHQGRALVRQEWVMEAAPGLIALSGGRDGEIGRLLLNDRPEQAEARLKAW
                     QQAFADRFYLELTRTGRADEELYLQRAVALAQRLACPVVATNEVCFLDRDDFEAHEAR
                     VCIQEGRTLDDPRRPRLYSEAQYLRSPQEMAELFHDLPEALENSVEIARRCTLELQLG
                     KNFLPDYPVPTDESEEAFFRRLAHEGLMQRFAQQSIDDEEKRKSYLQRLEFELDVIVQ
                     MGFSGYFLIVMDFIRWARDNGVPVGPGRGSGAGSIVAYALRITDLDPIGYELLFERFL
                     NPERVSMPDFDIDFCMDGRDRVIHYVSERYGREAVSQIITFGTMAAKAVVRDVARVQG
                     KPYSLGDRISKLIPFEVGMTLEKALKQEPALKELMAQNDEVAEIIEMSFKLEGITRNV
                     GKHAGGVVIAPGRLTDFTPLYVDESGGSLVTQFDKDDVEAAGLVKFDFLGLRTLTIID
                     WAVKAINQARASQGQPPIAIESLPLDDPEVFRYLQGGETTAVFQLESRGMKELIRRLL
                     PNCFEDIIALVALYRPGPLQSGMVDDFINRKHGRAAVRYLHPTLAPILANTHGVILYQ
                     EQVMQIAQVLAGYTLGSADMLRRAMGKKKAEEMADQRKSFTAGASQRGVPPAVAEEIF
                     DLMEKFAGYGFNKSHSAAYALISYQTAWLKHHYPAFFMAAVLSADMQHTDKVVLLVEE
                     CRKMGLRVSPPNVNRGEFRFSVDVEEGSIIYGLGAIKGLGEGPIDALNSARRSGGRFI
                     DLFDFCRRCDSHKLNKRALEALIKSGAMDELGSDRATLLATLPNALQAAEQSAANSAS
                     GTRDLFGELLPGSLISSQPVMQVSAQRWSLREQLQAEKETLGLYLSGHPIEQYRDELR
                     AMKVTQIADLQPDNGPQLFAGLLLGVRTLRSKRGDTMAFLTLDDRTARIEISLFADDY
                     QQQRDKLIKDEILLIEASVSLDEYNSSLKARAKSLSTLDEARTRLVRQLELDLRGDAL
                     PNDFDRQLAELLAPYRGDGCPVLLNYRGDAVGGRIHLGAAWRVRPTDQLLERLRGQFG
                     IDRVRMSYH"
     gene            36336..36983
                     /gene="adk"
                     /locus_tag="PKI23_02180"
     CDS             36336..36983
                     /gene="adk"
                     /locus_tag="PKI23_02180"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNN35778.1"
                     /translation="MRLILLGPPGAGKGTQARFICEHFSIPQISTGDMLRAAVKAGTP
                     LGQQVKAVMDSGALVTDEIIIALVKERIEQPDCQKGFLFDGFPRTIPQANAMVDAGVQ
                     IDHVVEIAADDEEIVRRISGRRVHAQSGRVYHVLFNRPQREGLDDVTGEPLIQRDDDK
                     EETVRNRLKIYHEQTRPLVDFYRKRAEQSASLHYHTIDGMGEVDQVRAAILGVLG"
     gene            37030..38049
                     /gene="hemH"
                     /locus_tag="PKI23_02185"
     CDS             37030..38049
                     /gene="hemH"
                     /locus_tag="PKI23_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNN35779.1"
                     /translation="MPQPTSVAQSPRRYGVLLVNLGSPAAPDAAAVRRFLRPFLGDRR
                     VVEIPRPLWWPLLNLFILPFRAPRVARLYQRIWMAEGAPLQVHLERLREAVTARFHAG
                     SSGGDRIEVRAAVTYGAPAIADQLDALMAAGSDEVIVIPLYPQYSATTTAAVFDAVSR
                     WMQQRRDLPGLTLVKDYHDHPRYIEALAQSVRTAQQQFGTPERLLFSFHGIPQANVDQ
                     GDPYARHCHRTAEAVAQALGLELGQWLVAFQSRFGPAQWLQPYMDEAVKALAAEGVRH
                     LQVISPSFSLDCLETLDEIDHEYRQLFLSQGGERFHYVPALNAGDSQVELLVELISCH
                     LPVAR"
     gene            complement(38076..>38502)
                     /locus_tag="PKI23_02190"
     CDS             complement(38076..>38502)
                     /locus_tag="PKI23_02190"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNN35780.1"
                     /translation="TYPFPYLHLSYPADVAIEKVRSALRAPCDLMATVITNLGEADEA
                     RLPAHILDISTRGMRLETTPNALQVGQPIHIEMELQIDEHRYAMTPAGTIRSMSEPGK
                     STSTADAERKIGYGIELDTLSRNNYLAIYSFVHACLAIA"
BASE COUNT         7560 a        10891 c        12283 g         7768 t
ORIGIN      
        1 gggtgccgcg acggcgatga aagcgatgat tctggcggca gggaagggca cgcggatgcg
       61 tccactcacg ctgcacaccc ccaaggcgct gctgccggtg ggcggcaagc cgctgattct
      121 ccaccatatc gaggcgttga ggcgttctgg agtccgtgac atcgtgatca actgcgccca
      181 tcttgggcag cagatcatcg atgcgctggg agacggtcaa cgccacggcg tgcggctgca
      241 ctattcgcag gagcccgacg aaccgctcga aaccggcggt ggcatcctgc aggcgctgcc
      301 gctgctgggc gacgctccct ttctgatcgt caatggcgat gtactgaccg acttcgactt
      361 cgcccgactc aggctgccac cgggaaaact ggcccatctg ctgctggtcg aaaatcctga
      421 ccaccatcgc gaaggtgact tctatctgtc gcccagcgca gcggtgcgga gccaggcgat
      481 gcccggcgcg cgccggctca cctacagcgg catcgccctg ctgtcaccgc agctctttgc
      541 gggatgccat cccggcgttt ttccgctggc accgctgctg atcgccgcca tgcagaacga
      601 tcgggtcagt ggtgaattgc atcctggacg ctggctcgat gtcggcacgc cggagcggct
      661 gcaacgggca gagcgtgaac tggccggagg ggtggcgtga agcgattttc catcgtcgcg
      721 tcccagccgc cctggatcgg caagttgctt ggggcgctga ccggcctgat ctgggcgccc
      781 gattcgcttc actggagcgg gctggtgctg ggtctgctgg ccggccatct gctcgatcag
      841 cgcctgggcg ggcttgcccg cgctgacaca tccgcgcaaa agaccgtggc cgcactgcgg
      901 ccgctgctca tgctgatggg ccatctggcc aagatcgacg ggcgggtcaa tgaggcggag
      961 attgccgtca ccagccgtct gatcgaccgc atggtgacgc aggggcgtga ccgtgccgaa
     1021 ctgatcgaac tgttcaggca gggcaagggt gccacgcctg ccgagatcga tcgacaactg
     1081 gacagactcg ccgacacgct gtgccaccag ccgaaactgg ccgagcagtg gctgggcatg
     1141 ttgatcgggt tggcgctgag tgatggagcg ctgtgtgcgg gtgagcgggc gttgatcgag
     1201 cgcattgccg tgcggatcga gatcgctccc gagacactgc ggcgcatcga atcacgtctg
     1261 cgtcgccagc agtcacgccc ggcggagccg gccaccaccc cggtttcggt gctgatgcag
     1321 gcctatcagc tgctgcaggt gaacagaaac gccagcgatg aagaggtcaa gctcgcttat
     1381 cgccgcaaac tgagccagca tcaccccgac cgtttgcagg cgggtggtgc ttcagcggag
     1441 cagatacagg ccgctggcca gcagacccat gagatcagga gtgcctacga actgattcgt
     1501 gctgcccggc tgttcaggca atcagagtga gcagcgccgc ttcgtaaccg agcagcgtga
     1561 ggtaaccgcc acaggcggtc cgcagcagcg tgctgaccgg aatccggccg aacagaacga
     1621 agttcttgtg gatggtcaga aagatcaccg acagcgcggt gatcagcagc tttgactgca
     1681 cgaaatagtc ggggtcgttg accagccaga atgccatcag ccagttcaac tcctgtgcgc
     1741 catgctcgat caggcgcagc gtcatcaagg catcgccagc tgctgcgatg atcagcagca
     1801 ccgcgatcaa cagatagacc ggctcgtaca ggtcaacgta gcggttgcgt gccagtcgcg
     1861 gctgtggcgc cgtgcgtctt cggccggcga tgcccagatg acgcaggttg atccatggtc
     1921 ggttccggcg ccgctcttcg aaaggtgttg ttgtcatgct ccaccctgtt caataccgag
     1981 tgcacgctgt gtcacgccaa tcaaagcaca gcgttaatga tcacacaata tccagatcga
     2041 tccttgtttg tacaattttg ccgcaggaga aggccaggac aaacagactg ggtgccggta
     2101 gaatgagcca ttttttcggg aactgccgtg gaatttctgc ttgcaccgtc cattctctcg
     2161 gccgacctgg cccgtctcgg tgacgacgtg gccgcgatgc ttgcggccgg caccgactgg
     2221 gtgcatttcg atgtgatgga caaccactac gttcccaatc tcacctttgg cccttcggtg
     2281 gtgaaggcgc tgcgcggcta tggcatcacc gcgccgatcg atgtgcatct gatggtcaag
     2341 ccggtcgatc gcatcatcgg tgacttcatc gaggctggcg ccagcctgat cacctttcac
     2401 cccgaggcga gcgagcacat cgaccggtcg ttgcaactga tcatcgatgg cggttgcgcc
     2461 gcagggctgg tgttcaatcc ggccaccccg ctgaaccatc tcgaccacct gctcgacaag
     2521 ctgtcgctgg tgctgctgat gtcggtcaac cccgggtttg gtgggcagaa attcattccc
     2581 ggcaccctgg ccaagatcga gcaggcgcgc cggcggctcg atgccgcgcc caatccagtt
     2641 cggctcgaag tcgatggcgg cattggcctg cacaacatcc gcgccgtggc cgaagcgggt
     2701 gccgatacct tcgtggccgg ttctgcactc ttcggcagcg atgactatcg tgcgaccgtt
     2761 gcggccatgc gcgccgagtt ggcccggatt gaacgcaatc cggcttgaga gtgtcatgcg
     2821 aaacggtcgc aacagtttga ccactgcccg taaggtgaat tgagcatgat tcaggtacga
     2881 cccgacccaa aaatcgatgc cgatgagccg acgctgcggt tggcggtgct gatcgatgcc
     2941 gacaatgccc aggctgccgt catcgaggcg ctgctggccg agatcgcccg ttttggcgag
     3001 gcgacggtca agcgcatcta cggcgacttc acctcgccca ccagtgcttc gtggaaaaag
     3061 gtgctgcagc gctatgcgat caaaccggtg cagcagtttg cctacaccac cggcaagaac
     3121 gccaccgaca gcacgctgat catcgatgcg atggatctgc tctatacccg caagttcgat
     3181 ggcttttgcc tgatcaccag cgacagtgac ttcaccggtc tggcgatgcg gctgcgtgaa
     3241 gaggggctca cggtgctggg gtttggcgag aagaagacgc cagaggcgtt ccgcaacgcc
     3301 tgccacaagt tcatcttcac cgaggtgctg cgtccggcga tgacagccaa gccggcagcc
     3361 gccgccacgc ggacggcgcg tcggaaaaaa tcggctccgg cacagactct ggccgagacg
     3421 aacgaaccga agctggagtt tccgaccgag ttcgtgctga ccgcgctcga tcagtcgatc
     3481 gacgatgccg gctgggcgca ggtgggcacc tttggcagtt atctgaccaa gttgcaaccc
     3541 gatttcgacg ccaggctcta tggctaccgc aagctttccg atctggtccg ggcccgaacc
     3601 gatctgttcg tgaccgaaga gcggcaggcg gcgggttcga gtcagaaggt gctctatctg
     3661 cgggcgaagt agtgcatgag ccgccatgct cccaatcggc ccggaagacc ggtcacagtc
     3721 agatgaaccg cagcgtcatg caggccatca tcaaaattcc ttatcaatcg cgatggcgtg
     3781 gcatccgccg ttgagtgcct gccgatgagt ctgtctgttg cgcccgttgc catggagttt
     3841 tcatgatctc tttgaccgaa accgagttca accgccttgc cgccgaaggt tacaaccggc
     3901 tgccgctgac gctggagacc tttgccgacc tcgatacacc gctgtcgatc tatctcaagc
     3961 tggccgatgc gcccaacagt tttctgctgg agtcggtggt gggcggcgaa cgctttggcc
     4021 gctactcctt catcggcctg ccggcccaca ccttgcttcg ggtgcgtgaa tcgatcaccg
     4081 aggtggtcag ccatggcgcg gtcatcgagc gccacgaggg caacccgctc gattttctcg
     4141 agcagtatca ggcgcgctgc aaggtccatg tgccggtcgg ctttccgcgc ttctgtggtg
     4201 gtctggcggg ctattttggt tatgacacca tccgttacat cgaatcgaag ctggccggtt
     4261 tcgaccatcc cgacgaaatc ggcatgcccg acatcctgct gctgcagagc gaagagttgg
     4321 cggtgatcga caatctggcc agcaagatct atctgatcgt ctacgtcgat ccagcgttgc
     4381 ccgctgccta tgcccagggt caggcacggc tgcagcaact gctcgaactg ctgcgcggtc
     4441 cgctgaccct tccgcagagt cgaccgagcc cggcgaccct gccgcagcgt gattttcgca
     4501 aggaggacta tctggcggcg gtgctgcgtg ccaaggagta catcgccgcc ggcgacatga
     4561 tgcaggtgca gatcggccag cggatcaagc aacccttcag tgactcaccg ctgaccctct
     4621 atcgcgcgtt gcgctcgctg aacccgtcgc cctacatgta ctactacgat ttcggcgatt
     4681 ttcaggtggt gggcgcctct ccggagattc tggtgcggca ggagtcgagc ggttcgggca
     4741 cgaccaaggt gaccatccgc ccgctggccg gcacccggcc gcgcggtgcc gacccccagc
     4801 aggatcgcca caacgccgag gagctgctga gcgatccgaa ggagcgtgcc gaacatgtga
     4861 tgctgatcga tctggcgcgc aatgacatcg gtcgcatcgc cgagaccggc agcgtcaagg
     4921 tcaccgacca gatggtgatc gagcgctact cccacgtcat gcacatcgtc tccaatgtcg
     4981 agggggtgct gaaaccggga accagcaacc tcgatgtgtt gcgggcgacc tttccggccg
     5041 gcaccctgac tggtgcaccc aaggtgcggg cgatggagat catcgacgaa ctggagccga
     5101 tcaagcgtgg gctctatggt ggtgcctgtg gttatctgag cttctcgggc gagatggacc
     5161 tggccatcgc catccgcacc ggcatcgtca agcagggcat gctctacgtg caggccgcgg
     5221 ccggcatcgt ggcggattcg gtgccagaga gcgagtggca ggagaccgaa aacaaggcgc
     5281 gcgcgctgct gcgtgccgcc gagcaggtgc aggccggact cgaccacgtc ccatagcgga
     5341 gtcttcgcca tggccaagct gctgatcctg tttcacagcc agagtggcaa caccctgcaa
     5401 ctggcgcagt cggtctaccg cggtgcgctg gcggtggagg aggtggagag ccggctgctg
     5461 cgtgcgaccg atgcgggact ggatgagctg ctctggtgtg atggcctgct gctggggacg
     5521 ccggaaaatt tcggctacat gtcgggcggg ctgaaggatt ttttcgatcg aacctactat
     5581 ccggcgcagg atcgggtgac cgcaaagccc tatgcgctct tcatcagctg cgaaaacgat
     5641 ggcaccggtg cagtgggcca gatcgagcgc attgcccgcg gctatgcgct caagccggtt
     5701 ctcgaaccgc tgatctgccg aggtgcggtc tcggcgtcat gcctggcaca ggcgcatgaa
     5761 ttgggaatga cgatggcggc cggactggat atcggcattt actgacggtt gcgccgcggt
     5821 ggggagaaat ccaggcagaa gaaaaagcag gcgggaaggg gaggggtcca ctgcgggcca
     5881 aagcccgcag tggcaggagt cggtcagggc ttccagggac ggcaggtggc gttgctctcg
     5941 atcagcactt cacctttctg gttgacagcg gtgaggtcga gatcgacatg cttgacgccg
     6001 ccttcgctgt agaccttggt ggccacaccc ttgaaggtga catcatcgcc cggccacagg
     6061 cgggtcttga agcgaaaacc gtacttcttc accgaggtgg cgccgaacca gtcggtcagc
     6121 acccgtgcca gcatgcccga ggtgagcatc cccatgccaa agaccgtcgg caggccggcg
     6181 gactcggcga aggtctgatc atggtggatc gggttgaagt cgccaccggc gccggcgtac
     6241 ttgacgaact gggtgcggtt gaaattcttg acgacgaagc tgtattcgtc accttccttg
     6301 attttgtcga atgctgaatc agccatcttt gaccactcct ccagtctgca atacggtgtt
     6361 cttgaccttg atcacgacgt tgccgttttg atcgacatat tcgctgaaca tgtcgaagat
     6421 tttcatctgg ccgccgcgct tgccttcctt gacgaaggat ttgacttcac cggtgcgcat
     6481 cgtcagcaca tcgccggcaa agagtggtcg gacgaactcg aactcctggc caccgtggag
     6541 cacgaagcgc aggtcaagac ccagactttt gatcgcgctg ccatcatcgc cgctggaaaa
     6601 atgggcggag gtggcggtgt aggtcggagg ggccgggact gaaggcagac cggctgcctt
     6661 ggctgcgacc tcgtcgtgat acatggggtt gtcgtcttga agcgagttgg caaattcatg
     6721 gatcttgcca cgctcgatcg gaaatcccgg aggattgatc gtagccataa gcgtccgttc
     6781 tccttgtttg acagatgctg ttgttatcgc atcgccgggt ggcgatgtgc tgttgttccg
     6841 caccaagcat ttctggccac cagaagtgaa cactgcctga cggcaagttg ctggtgcgtg
     6901 gcctcgacga ggagccgcaa aagtatcatc ggcgcttcgc ccgagtcaat tgccccatga
     6961 cccggtgtca ctcgatggct gccgcgacat cgggttcgcg tgcaggcacc ttgaccaggg
     7021 cgatggtgat ggcagcgatc agcagtgcca ggatcatgat caggtaggag atgcggtagc
     7081 tgccggtttg atcatagatc cagccggcca gcgggaccgc gcacatctgc agcggaatca
     7141 ttgccggtcg gatcagaccc gaaactcggc cgaacgacag gcgaccgaag atctgtccgg
     7201 tggtggaact ttgcagtggc acgacaccgc ccatgccgaa accgaacaag gcgccaccca
     7261 gcaacagcca gaggtaactg ctgcagagca tcagcaggat cgcgccaagc agttgcgtga
     7321 tgatgcagag ccagaccgcc aacttcggct cattgtggtc ggtcagccag ccgaacacca
     7381 ccttgccgat cacgccaatg cctgccgaga ccgagaggat catggcggcc tgggtggtcg
     7441 aaagcccctg gctggtcaat tgcggcacca tgtggatcag aatggcctga aacaccccga
     7501 ccagcaacgc gaaggtcagc gcaatgaacc agaaattgct cgatcgcaga atggcagccg
     7561 tgctccattc gacttcggcg ccacccagaa gggtgcggtg ctgcgcgtca gtggcaccgt
     7621 caggccgttg gccaagttct tctggattgg atctgaccag cagtgccacc acgggcgtca
     7681 cgcaaagcag ggcaacagtg cagtagacca ggtagccacc gcgccagccg atatgatcga
     7741 tcagccaggc ggagagtgtt ggcatcacca ggcccgaggc ggagatgccc atggtggcga
     7801 tgcccagcgc cagaccacgc ttgcggatga accaggccgc aaccagtttg gaagaggcct
     7861 gcccgcccat ggccgcacca ccaaaggcat tgcaggtggc gagaatcaga tagaactgcc
     7921 acagtgccga aatggtcgag agcaatccat aacccagtgc cagtaccgtc gcgccgagca
     7981 gcatgatcca gcgggtgggg tagcggtcga gcgcccgtcc gagaaagggt gccatcaggg
     8041 cgccgctgac attgctgatc atgaaaccga gcgacacatc caggtgactg ccgccgaact
     8101 ccttgctcag tgcaagaaag aagatgccgt aggagtagaa gaaaaagccg accgagatga
     8161 agtcggtaaa gagggcgatg gccaccatcc gccagccgtg aaatggaggc tttgccgaag
     8221 ggtcggcagg attttcaggc agaggctgca tgggtgggct cggacaggat ggtgcgggag
     8281 ggtgaatcta tccgttcatg aacagaactg ccatgaaaag cacatcgacg ctgcaatgga
     8341 aaacggtccg gatggtggag gcttgcgaag gtttcaattt cctgcagctt caatggaggg
     8401 tgttgatcga accggtggtt tcatccagcc gacgcagagc gcgcagagtg cagtcaatgc
     8461 ggccagagag atgaacaccg tctgataact gccggtgcgg tcgaagatgg ctcctgcggc
     8521 gatgggtccg gatgcggcaa ccagcgtgta ggagagcgcc tgtatcgcag cgatggcgga
     8581 aaagttgagc gcgccgaaat agttggcggt cagcgccgtc agcgcgatca ggtagacacc
     8641 gatacccatc ccgaacagca ccgtgaacag atagaccagc ggctgggagg tggcgaaatt
     8701 gagcaggaga aacccgatcg cctggaacac catgcctgcg gccagaatcc accttggatc
     8761 gatgcggtcg ccaatgccgc cgctgagaat gcgtcctgtc gagttgagga tggcgaccat
     8821 ccccagactg ctcgcagcaa ccagcgatga gacaccgatg tcggacagat gcagaatcag
     8881 atgggcgttg atggttgccg aagcagtggt ggccgcacag gccaccagaa cggtgagcca
     8941 gaaaaaacgg gtgcgcagcg cttcgcgcag ggtccagttt tcactggtct tgaacacggc
     9001 attggtcgag gttgcggtga catgcgcctc agcggggttc ttgacaccgt ctggatgctg
     9061 gccaatctcc tcaggccgtt ctcgaatggc ccaaaagccg aggaggccga ccagcaggcc
     9121 gcagacagcc atcagcagcc agacgatccg ccagttgccg gtctgctcca ccagtgccgc
     9181 gaccatgggt gaggccacga agcctcccag tccaccattg gccatgaaca gccccatcgc
     9241 cagtgcgcgg cgctgcacga accaggtcac cagcagattg cttccaggca gcacgctggt
     9301 catggagaga ccgatggcga tgaagaagcc ggtacccaga tagaaaatgt acagagagga
     9361 tgtctggtag atcagcagag cgccgcagac gatcaggatg ccgcccgtca gataggtctt
     9421 tcgtgtgctg aatcggcgga tgaagatcga caccagcgga gcactgacgc cccacaccag
     9481 cagcagcaga gagaatccca ccgaggcctg acctcgactc cagccgaact cctcgatcat
     9541 cagcggcagc acgacgctga ggccgtacat gcacagcccg accccgagga aatagcagag
     9601 gctgatcacg gccagaagaa cccatccata gaacatcccg tcattttttt tgcgcatggg
     9661 tctgcctgtt tttattgttt tcaaagaatg ttctggaaag tgtgtcgtca agctgcgttg
     9721 cgcggcatcg aatgccgcaa cccttgacgg tttgcattat tgcacgaaaa aacagcacat
     9781 gaaagttttt tcatgccggt ttcgatgcag gcgcccgatg gtgtcgagga gccatccgct
     9841 gcggccgtca caactgcttt ttttcttcct cggtcaacag gcgggcttcg gactcgagca
     9901 tgatcggaat gtcatcgcgg atggggtagg cgagaccgct ggctttgcac agcaactcgt
     9961 tgcgctcctt gtgatagacc agcggtgcct tggtgacggg gcagaccaga atctgcagaa
    10021 gtttggggtc cagcatgaag gggctcgtga aagatgaaag gtagggcggc gggattatag
    10081 caatgcgcac gactgggcat catccttcag aagcgtggtt ttgcgcagtc gatgccgtgc
    10141 tcttggcatt gtcgctacaa tgaccgttcc gtcaccttga gaaaccgcca tgagccgtgc
    10201 cgaccacctg atctggatcg atctggagat gaccgggctc gatcccgacc gggaccgcat
    10261 catcgagatc gccaccctga tcaccgatgc cgatctcaat gtggttgccg aaggtccggt
    10321 gatggcgatt caccagtccg atgaggtgct ggcggcaatg gatgagtgga accagcacca
    10381 ccatggcgtc tccgggttga tcgaccgagt ccgaaccagt ggtgtcgacg agcaggatgc
    10441 cgagcgcatc acactcgatt ttctcggccg ctatatcgag ccacggcgct caccgatgtg
    10501 cgggaacagc atctgtcagg atcgccgctt tctggcccga cacatgccac ggctggagcg
    10561 gttctttcac taccggaacc tcgatgtcag caccatcaag gagctggcgc ggcgctggcg
    10621 tccggagttg ctgaatggcc tgaacaagcg ggccacccat gtcgccatgg atgatgtcaa
    10681 ggagtcgatc gccgaactca gccactaccg ggcgcagttc tttcgcatga atgaggacgg
    10741 gtagtctgcg ctgagtcggg ccttctgcgc tcttttttgg agcggacgga ggctcgacct
    10801 tggcgcaaag ggcgggtccg agctcgactt tcgatcgttt tgcacgctcg atcaccgttt
    10861 tttccgattt ttccagttgg caggaggatt gcctgtcacc gttgagactg gaagaaacgg
    10921 agatcgagga tgagtacgat cactgcacct cccgccatga cccgttggct tgccgggtca
    10981 cggtgggcgc cggatttcgt ttctggaggg gccgatcctg cgttgttctg cgactgctgt
    11041 gcagcggggt cgggagttcc atcgctgacg gcattgcctg cggcattgca ggaggcgcgg
    11101 cgtgccgaga ccctgtccct gatccgggcg caactgcggc acttcgcgct gctgctgtac
    11161 atgaagggca agcccgatca accgcgctgt gcgcgttctg cccgcgtcgt ggcgctgctg
    11221 cgccatgcac aactgccgtt tgccttcgtc gatgtgctgg cacagccgtt gattgatgag
    11281 ttgttgcagg agttttctgg cctgtctggc tacccgcaac tgtttctgga tgggaacctc
    11341 gtgggtggtt atgcggccat cgcgcgtgcg atcgaggatg gcacgctgtc gcgctggatc
    11401 gaacagcgtt gccgtgccga ggaggtggtg attccatgac gccgcaacag atcgagcact
    11461 gcatcattga ggatctgccc gatgcagtga tcgatgtgga gatcaaaaga ggagcgaccg
    11521 ttctgcgagt ggtgagtcag aagtttgctg gccgagggca gtcggaacgg cagcgtcatg
    11581 ttctctcact gctgaaacag gggctcgcga acggcagcct gcacatcagt catctcaagc
    11641 tttattcgca tgatgagtgg gcggtgctca agccgaccga agagattctg ctgatacaac
    11701 ggaacggctc atggccagcg tccagttaag acgctatgat gcttcagcat aaataacaac
    11761 aagatagggt tttatcctca tgctgacagc tctgttgcga cgcaaatcga tcagccaggc
    11821 gctcgcgctc agtgaagaga atgggctgca ccgtgcgctg ggtcggttcg acctgatctt
    11881 tctgggcatt ggtgcggtca tcggtgccgg catcttcgtg ttgaccggca ttgctgcagc
    11941 gaccaaggca ggaccggcgt tgtcgctctc attcgtcgtg gccggattgg cctgcttttt
    12001 tgccgcgctg gtctatgccg aactggcctc gacggtgccg gtgtcgggca gtgcctatac
    12061 ctactcctat gtgaccatgg gcgaattcat tgcctggatc atcggctggg atctgattct
    12121 ggagtatggc gtggcctcgg cggcggtggc catcggctgg tcgggctact ttttgcagct
    12181 gtcgaccgcg atgggactcg atctgccgga gttgctgacc aaggcaccgc acgaaggtgg
    12241 ctggatcaac ctgccggcgg caggggtgat catgctggtg acgctgctgc tggcggcggg
    12301 cgtgcggcag agcagccggg tcaacaatgt ggtggtgctg gtcaagctgg ccgtgatcgg
    12361 cctgtttctg gtggtggcgg cccccaaggt gcaggtcggg aactggcagc cgttctttcc
    12421 gttcggctgg cagggggtga tggggggggc ggcgctgatc ttctttgcct acatcggttt
    12481 tgatgcggtc tcgactgcgg ccgaggaggc gcgcaatccg cagcgggatc tgccgctagg
    12541 cattctcggg tcgctgctgt tctgcacggt gatctatatc gccgtggcga tggtgctgac
    12601 cggcgcagtt cactacggtc agctcaacgt cagccatccg gtggcccatg cgctggagct
    12661 gatcggcagt ccgaccgctg ccggactggt gagcgtcggg gcgattgccg ggctctcatc
    12721 ggtgctgctg gtgctgctct atggccagag ccgcatcttc tttgccatgt cgcgtgatgg
    12781 gctgctgccg gtggcctttt cccaggttca tccgcgcacc cgcacaccgg tcaccaccat
    12841 cattgccacg ggtgtggtcg tcgcgctggt ggccgggttg ctaccgatcg gtgcagtggc
    12901 cgaattggtc aatgtgggaa ccctggcggc cttcatgctg gtctcgatcg gtgtgatcgt
    12961 gctgcgacgc acccaaccgg agctgaaaag accctttcgc acacccttta tgccgtggac
    13021 gccgattctg gcgattctgt tctgcggcta tctgattgcc agtctggacg cggtgacgct
    13081 gctgcgtttc gtggtctgga tggcgatcgg catggtgctc tacttctgct attcggttcg
    13141 ccacagcacg ctgggcaggg cagccgccaa cgactgaccg cggtcatgcc tgtcaaccca
    13201 gctcgaggta gcgcagcaga tcagcctcgg acaggggacg gctgtagaaa taaccctgga
    13261 tgccgtaaca gccctgttcg cgcagaaatc ggacctgttc ggcccgttcg accccttcgg
    13321 caatcagttg cagattgagg ctcttgccga gggcgatgat ggtgttgacg atggcggcgt
    13381 cttcggggtt gctgtcgaca tcctggatga aggagcggtc gattttcagg atgtcgatcg
    13441 ggaagcgctt cagatagctg agcgatgagt agccggtgcc aaagtcatcg atcgagatct
    13501 tgatgccctg ccgatgaatc gcctggagcc gatcgatggt ctcctggaca tcctgcatga
    13561 tggcccgctc ggtcaactcc agttcgatca actcggcggg tacctgatgg cgccgcatcg
    13621 tttcgagcag tgagtcgagg aaggtggcat gggtgaactg gtgggccgag atgttgatgg
    13681 ccaccgggat gctgtagccc tgtttttgca actggctttg cagtcggcac acctcttcga
    13741 tcacccaggc gccgatcggc gtgatcatgc cgctctcttc ggccagtggg atgaagtggt
    13801 tgggagggat ccagtcgccg ctctcatcct gccagcgcag cagcgcctcg atgccgatca
    13861 cctgatgttc acggatgtcg atctgcggct ggtagtgaag ctggaactgg ccattgacca
    13921 gcgcatggcg cagcttgttg ccgagggcca actgatgcag tgactcctgt cccatgctgg
    13981 gctgatagag caggtgacga ttgcgtcccc gttgcttggc ctgatgcagg gcgatttcgg
    14041 caagttgcag cagcttttcg ggctccgcgg catggtcggg atagcaggcg acgccggcac
    14101 tgaaggcgag cagcagttca tgctgtgcca ccttgaacgg ctcgctcaac gccctgctga
    14161 tgcgctcgat gatggcgttg atctggctct gggcattctg tccactcaac aggacgcaaa
    14221 actcgtcgcc gccgaagcgg ccaacgtggg agatgtcgct gctttgcacc aggcgcgaga
    14281 gggggtcata ttcacggaca ttgctggcca gacgctgtgc cacccgcttg agaatcaggt
    14341 cacccacggc atggccgagg ctttcgttga tccgtttgaa aaagtcgatg tcgagcagaa
    14401 tcagcgccat gctggagccg tcgcgctggg cgcgctggat ttcgcggcgc acctcctcct
    14461 gaaaatgctc ccgggttggc agatcggtca gggcatcgta tgtgctggca tgttcgagct
    14521 ggtcgttgcg ctccgtgatg agcttttcga gatagtggtt ctggcgctca acttggtgat
    14581 agagccgcac attgtgcagc gcataggcac aatgctggag cacggccatc agcagccgct
    14641 gtgcccgagg cggcagggcg cgttcatcct cgaaggtggc gatcatcatg ccgagcgtct
    14701 ggttgggcac ggcgatcgga caggccagga cgttgacctg ctccttctgc ttgtagaggg
    14761 tgggcaactg ctgcttgacc gcccagccaa actggccatt gtcgatcaac tgatcaagca
    14821 gtcggggcag ggtgacatcg aagctgccgc tgccgacgcc acgcagattg aagttgaggc
    14881 cctcttcgtc ggtcagtgca aaaccgatgg cattgaacgg agcaatacgc tgcaaaaaag
    14941 ggagagtggc tcccagtatc cgttcactgt tgccatgcgg aaggcagtgc tgatagacca
    15001 cggcgatcga agagagaatg tccaactcgt tcgcatacca gcggttgagc tcttcgagaa
    15061 aatcgacgcg ctcctgcagc ttgccagccg gcagctcgag tagtgtgttg ggctcagcca
    15121 tcggtttcgc ccagcagcag tggttcgaca tcctggtact gtcgctcgag ttcattcagc
    15181 aggctctcca gtgtcgctgc acacagacca agtgtccgca gtgcaccggg gtcgagccgt
    15241 ggcgcaaaat ggtcgccggc atggccgaac gcggcaccat ccgccagcca gttggccagg
    15301 tggacgatgc aggccaccac actggtggtg ggcgcgatcg agggggcgat gtggtggcgc
    15361 acgccttcgc agtgcagggc cgggaggttc cactgcttga gcagtgcatg gccgatttcg
    15421 gcatggtgaa agcccagcag ctgctcctcg gcatcgctca gcagcagacc ggcttgctgc
    15481 gcctggatca gagactgtgt ggccagttcg ggatggtcgg taaagagcac caggcggccg
    15541 atcttgtgga acagtccggc aagaaagaac cgctcgctgt cgcgttcgcg cagatggatg
    15601 gcgatgaccc gcgcggcgat cgcggtggcc agcgcattgc gccagaattc acgcatgctg
    15661 agctgcgggg tctccagcag ccggaagcgt tcgatcacca gcgtggccag cgccagctcc
    15721 tgcagttgtc gggtgccgat cagcgtcaag gcccgggtga tggtttcgat cgggccgctg
    15781 tgtcgataga gcgcgctgtt ggccagtttg agcagcctgg cggcgaggcc aggttcgttg
    15841 ccgatgatcc gcgccaggtc atccagtgtg ctggcaggcg agagaatggc ggcttccaac
    15901 tgctgatgca gttcgggcag cgacgccacg gtggtggtgt tggccgcaat cgattcagcg
    15961 ctgagcggtg ccatgcagat atcccaactg tttggccacc cggttgaaga ccagccgttt
    16021 catctcttca acggccggat ccgctggatc gttgtggcga aagcgttcgc agacgatggc
    16081 ttccaccagc aggctctgtg cctcagggag ttccaccttg ccgggccgca gcgcctctgc
    16141 accatccacc accacctcgg tgatgcccca actgcgcagc gccttgagtt tgcgctcatc
    16201 cagggtgcag tcggcgggca gcaggatgcg tccgttgcca tccttgatgt cgtggatgat
    16261 gcgcatgccg ggttgcgcgt gctggatcga tagagactgc atcggtgttg gcgctgtttg
    16321 tgattggagt cgctgaccct ctttgagaaa tagcatagtc aataattttc tctggtacag
    16381 aattcagtcg tttcaggttc ggtcgacttt ggttcgatgc gatgaatgtc ctgctgaaaa
    16441 agcgggaaca catcttgctg aacaggggtg aacggcattt cggcgtcaaa aaaccgtgat
    16501 gcctcaccgc tgctgaacag agacaaaccg gatgaacact cgacttgatg cgggcccggc
    16561 acccattgcc tggcggcgtc tgctgcaggg cagcctcggc attccgctga ttctgctggc
    16621 gctgctgggc atgatggtgc tgccgctgcc ggcggcgttg ctcgacctct tcttcacctt
    16681 caacatcacg ctgtcgatcg cggtgctgct ggtctgtgtc tacgccagaa agccgctcga
    16741 tttcgcggtt tttcccacgg tgctgctgat caccaccctg ttccggctgg cgctcaatgt
    16801 cgcctcgaca cgggtggtgc tgctgcatgg ccatgagggc ggtgatgcgg ccggcaaggt
    16861 gatcgaggcc ttcggcgagg tggtgatcgg tggcaactat gcggtggggt tggtggtctt
    16921 tgccattctg atgatcatca actttgccgt ggtgaccaag ggtgcggggc gcatctccga
    16981 ggtgagtgcc cgtttcaccc tcgatgccat gccgggcaag cagatggcga tcgatgccga
    17041 cctcaatgcc ggcatgttga cgcaggatga ggcccagcaa cggcggcggg aggtcgcaca
    17101 ggaggccgat ttctacgggg cgatggatgg cgccagcaag ttcatccgcg gcgatgcgat
    17161 cgccgggctg ctgattctgg tgatcaacct tctgggtggt cttggcatcg gcatgttgca
    17221 gcatgatctc ggctttgccg aggcgatgag ccgctactca ttgctgacca tcggtgatgg
    17281 tctggtggcg cagttgccct cgctgatgct ctcgattgct gccgcgatca tggtgacgcg
    17341 ggtcgagagc atgagtgaca tgagcgaaca gatcgtcgcg cagatgttca gctccccaag
    17401 aattctgctg gtgaccgcgg cggtgatggg ggtgatcggg gcgattccgg gcatgcccca
    17461 tctgattttc ggtgtgctgg cggcggcgac cggcgcgctg ggttattggt tggagcagaa
    17521 aaaacagcgc gcgctgttgg caccggtgac cgatgaggcg ccagtggttg cagtgacgcc
    17581 cgaagtgcgg gagctcggtt gggacgatgt gacgccagtc gatgtgaccg gcctcgaggt
    17641 gggctatcgg ctgattccgc tggtcgacag cacccagggc ggtgaattgc tggcgcgcat
    17701 caagggcata cgcaagaagc tgtcgcagga tcttggattc ctgattccat cggtgcacat
    17761 ccgcgacaac ctggagctgc cgccgaacca ctaccgcatc attctgatgg gggtggcagt
    17821 ggcggaggga cggatcgaga tggggcggga gctggcgatc aacccgggca aggtgttcgg
    17881 caccctgccg ggcatcgaga cccaggatcc ggccttcggc ctgccggcgg tctggatcga
    17941 tcccggccag cgtgagcggg cgcagtcgct gggctatacc gtggtggatg gcagcaccgt
    18001 ggtggcgacc catctcaacc agattctgct ggagcatgcg gtcgacctgc tcggacatga
    18061 agaggtacag aagatggtcg atcttctgat caagtcttca cccaaactgg ccgaggagct
    18121 gattcccaac cgcatcagcc tcagtctgct gctgaagata ttgcaaaacc tgctgcgcga
    18181 gcgggtgtcg atccgcgacc tgcggaccat cgccgaagcc atcacctccg tgacaccacc
    18241 gagtcaggat ccgaccgtgc tgaccgccgc ggtgcgcatt gcgctgtcgc gcatgatcgt
    18301 tcagggattg gtcggcaatg gcggtgagat tccggtcatt acccttgaac cgcaattgga
    18361 acagatgttg ctttcttcat ataaacaggc gcgcgacgcc ggaaatccgc cggaagaggg
    18421 gatggtgatc gagccgagcc tggcggagcg gatgcagcgg atgctgcgcg aagccgccgc
    18481 gcgtcaggag atggacggcc agagtgcggt gctgctggtg tccgcaccgc tgcggccagt
    18541 gctggcacgc ttcgtccgtt acggtggcgt gtcgatgtcg gtgctgtcgt accaggaagt
    18601 gcctgaaaac aagcagatca tggtggtcgc gaccgtaggt cgtcactgat gggttgtctg
    18661 aagctggatc aatgtcatgc agataagacg ttttgttgcg cccgatagcc gcactgccct
    18721 gcaacaggcg cgtgaagcct tgggaccgga tgcggtgatc ctggccaacc gcaccctggc
    18781 caatggcgtc gaaattctct gtacagcgga ttacccgctg ccgatgcaac cccaaggcga
    18841 tgctccgctg gcggcgccga cgatggctgc ggcgccagtg caacagccag ccgaacctgc
    18901 cactgggact gccacagcga tggcatcctt tgtccctgcc agcgccacca ccgatacagc
    18961 tcaaggaaca gcgagcgcag ccgtcggcca gagcagagtg ccgagcgcga tccgcttccc
    19021 cagaacgggt cagttccgtc tgggtgagaa agtggcatca atgttgacca aggagttcca
    19081 gcaggcgaga ccacagaacc agcaacctcc cacgcagcag ccgatcgcaa cgcccaaggc
    19141 tgctgaactg ccaaagggtg ctgcggagaa tggagcgcac gcagcggtct cgacgcagtg
    19201 ggccgaacgg catgaactgg agcagttgcg caacgagttg cagggggtgc gtgagttgtt
    19261 gagcagacgg atcgttgcgc ccgctgcggc accgctgcat gcgcagggcg aatccattcc
    19321 gccatccccg tggcgccgca cgctcgagga gagactgcaa cgtcttggtc tgtcagcaag
    19381 gctcgctgcc agactgctcg atcggtttga cgccgacgtg tcggctgagt cggagctggt
    19441 ctggcgtgaa gtgctggggg aactggttgc ccgaattccg gtcgtcggcg aagaccgcgc
    19501 catgcagggt ggcgtgatcg cgctgattgg tccgaccggt gccggcaaaa ccaccacggt
    19561 gggcaagttg gcggcacgtc atgtactcag gcatggggcc gatcaggtcg cactggtcac
    19621 caccgactgc caccgtgtcg ccgctcatga acaactgcgc atctttgggc gaatcctcga
    19681 catcccggtg aaggtggtcg atgagcgccg tgatctcgac accgtgctgc acagcctgcg
    19741 tgaccgccgg ctgattctga tcgacaccgc tggtctgcat cctgcctccg agcagttcgc
    19801 cagccagctt gccacgctgt cgagtgccag aacccccatc gacagctatc tgttgctgcc
    19861 ctgcaccagt cagcgttcgg tgctggagcg gaccgtcagg cactacggcg ccatcgatct
    19921 ggccggggtg atgctgacca aactcgatga ggcgtgcagc ctgggcgagg cgctggaggt
    19981 ggtgatcgaa cggcagtttt cgatcgccta tgtcaccgat ggccaatcga ttcccgatga
    20041 catcagactg ccgagtgcat tggaactggt cagccaggcc gtcgacctga tggaaaccga
    20101 acagaacggc atgaaacagg atttgatctg ggcatctccc gcctctcagg agagcacgct
    20161 tgccgtgccg gcctgagcgg tgccatgccc gactcaccac tttgtcagga gcatttcgca
    20221 ggatgaacag ttttcacccg gtacaggtca ttgccgtgac tggtggcaag ggcggcgtcg
    20281 gcaagaccaa tgtctcggtc aacctgagtc tggcgctggc ggatctcggt cgcagggttg
    20341 cactgctcga tgccgacctc gggctggcca acgtggacat cctgctggga ctgacgccga
    20401 aaaagaacct cgccaacgtc atcaatggtg aatgcggact caaggaggtg atgctcgatg
    20461 gtccgaacgg catcaggatc tttccggcct catccggcac gcagaagatg gtcgggctcg
    20521 atccgcgcga acatcttggt ctgatccacg ccttcagcga actggatgac gagcttgacc
    20581 tgatggtcat cgacaccgcc gccggcatct ccgacagcgt ggtcagcttc gtgcgggcga
    20641 gtcaggaggt gctggtggtg gtctgtgatg agcccacctc gatcgccgat gcctatggcc
    20701 tgatcaaggt gctcaaccgt gaccatggtc tcagccgctt ccgtattctg cccaacatgg
    20761 tcaaatcgcc gcaggaggcg agccagattt tcgccaagtt gaccaaggcg accgaccgct
    20821 ttctggatgc ctccctgcaa ttgtgcggct tcattcccta cgatgacttt ctgcgcaagg
    20881 cggtcaagcg ccagcgcgca ctctatgagc tctatcctgg cgccatggcc tccaaggcct
    20941 ttcaggagct ggccaaaaaa gtgatcgcct ggccggttcc gaaaacaccc agcggccatt
    21001 tgcagttctt cgtcgagcgg ctgctggcgc cgctgcgggc gactggctga accgtgcgcc
    21061 acacgctcta cgccgaggcg tcgcgctgca cgaccagtga actggtgacg cgccacagcg
    21121 aactggtcaa acgcattgcc tatcacctgt tgtgtcgcct gccaccgacg atacagatcg
    21181 aggatctggt gcaggcgggc atggtggcgt tgatcgaggc agcgcagaag ttcgatgccg
    21241 gcaagggcgc cagcttcgaa acctatgccg gcattcgcat ccgtggcgcg atgatcgacg
    21301 aggtgcgcaa gggcgactgg acgccacgct cggtgcaccg caacgcacgt gccatcagcg
    21361 aggcgatcca gctgctggaa aatcgccttg gcagagaggc cagagagagt gaaatcgccg
    21421 cggaactggg tgtggacctt gccagttacc acaccatgct gcaggaccag gctttcgcca
    21481 agctgcaaag tttcgaggag ctgctgcccg aggccgaaga ggatgagggt gatgagttcg
    21541 atctacccgg atctcatgcc ggaccacagc aacagatcga gcgcgagcac ttcatcgccg
    21601 cgctgggcag tgccatcggc cagttgccgc agcgcgagca gctggtgctg tcgctctact
    21661 acgacgaaga gctgaacctc aaggagatcg cggcggtgct gggcgtcacc gagtcgcgca
    21721 tcagccagat catcagccag gccgcactgc ggctgcgggc gcgtctgtcg agctggcgat
    21781 caagctaacc tgctgtttca aaggataaat accttcccgg ccgtcggcgt ctctgtcggt
    21841 ggcttggcca tcgtgcgtgc ttctgcctgt cagatgcatt tatccgggta aaaaactggc
    21901 cagctcgcca tccccgtcta gacttgtgga agtgggtatg acgcctgatg ttcggattgc
    21961 agcgagtgga ggtttccttg gacaagaaca tgaaaattct catcgtcgac gacttctcga
    22021 cgatgcgacg catcatcaag aacctgttgc gggatctcgg tttcaccaat accgatgagg
    22081 ccgatgatgg cctgaccggt ctgccgatgc tgcaaaaggg caactacgac tttctggtga
    22141 ccgactggaa catgccaggc atgaccggtc ttgatttgct caaagcggta cgtgccgacg
    22201 agcggctgaa gaatctgccg atcctgatgg tgacggcgga ggcgaagcgt gagcagatca
    22261 tcgaggccgc ccaggcgggg gtcagcggtt acgtggtcaa accgttcacg gcagccacgc
    22321 tcaaagagaa aatcgagaag attttcgaac gaatcgcggc ctgatcacga agatgggagg
    22381 ctaatacaat gattcagatg gaacagcaca tcgagcgtga cgaaaccatg gagcatctgg
    22441 tggacaaggc acgttctttg gttgaaaacc ttgaaagcgg caatctctat gacacgcata
    22501 agttgctggg tgacatctat gccgcgcggg agagaaagct ctatgaagag gtgggaaaac
    22561 tcacccgtgg cctgcacgat gcattgatgg attttcacat cgacgccggt atcggtgaag
    22621 aggcccaaga ggaaatctcg aagatctcgg atgcatcgga ccggcttggc tatgtcatca
    22681 aaatgaccga tgacgcggcc aatctgacga tggacaaagt cgatgaagcg gtgccgatcg
    22741 cggatgaaat acgttctacc tcaaaggacc tgaatgaaga ttggaagcga ttcaaaagaa
    22801 gggagctgac tcccgaggag ttcaggaatc tttaccagcg cgtgggtgac tacctcgacg
    22861 gcaccgaagt cagagcagag cggatgcaga attcgctcaa cgacattctg atggcgcagg
    22921 gctatcagga tttgaccgga caggtgatca agcgggtcat cacgctggtc aaggaggtcg
    22981 aaaacagtct ggtggaactg gtcagaatgg ccagcagcgt agagaaaatt gccggaatca
    23041 atgctgagac aggcgacagt gtcgagcctc aggaagtcgc cctggccgga cccaatgtcc
    23101 atggagaaaa aaatgtgacc gtggtttccg gacaggatga ggtcgacgat cttctctcca
    23161 gccttggatt ctgaggggca gcatcatgaa ccatgaagat gaagaaattt tgcaggattt
    23221 tctgctggag gctggagaaa ttcttgagca gctctcggag caactggtgg cgcttgagcg
    23281 tgatgccgac aacatcgatc tgctgaactc gatttttcgt ggttttcaca ccgtgaaggg
    23341 cggtgcgggc tttcttggtc tgaaaccatt ggtagagatc tgccacgtta ccgaaaacct
    23401 gttcgatgca ttgcgcaaag gacaatgcag aatcaccggc gaattgatgg atctgacttt
    23461 gcaaggtctt gattcaatca atgtcatgta ccagcagttg cagcagcgcg aagagcttgc
    23521 tgccgccaat tctggacttc tggcacgaat caaacgtctg gttgagggtg aacaggagtg
    23581 cgtggcgccc aaaaaggagg ttgaaactgc cagaccggca gctgcggtac cgcagaaaaa
    23641 agtggctggt tgcgccaatg acgtggtcga gcaagcgctg cgcgagatgc tggatgaagc
    23701 cgaagcacca gccgcccaag gtctgtctgc agcagagcgt gatgagatca ccgacgatga
    23761 gttcgagatg ctgctcgatc agttgcatgg caagggaaaa catggcggtg ttccggccaa
    23821 caaaacggac ggggtgaaaa tcgacaacaa agcggctggc agtgatctga tcaatgatga
    23881 tgagttcgaa gcggtgctgg atggcctgta tggaaagggc aaaggtccga ctttcgagca
    23941 aaagtccggc aaaacccggc acaatgatgt cgtttcgacg ccggtttccg ccctcgaatc
    24001 atcttcatct tctggcattc tgaaaaaggc agagccggtc agtgccgcca gtgcaaaaga
    24061 gacaccggcg gccgctgccg agaacaccgt tcgggtcgac acgcatcggc tcgacgacat
    24121 catgaacatg gtcggcgaac tggtgctggt cagaaaccgc ctggtccgtc tcggtgtcga
    24181 cagccatgat gaggagttga gcaaggtcgt cacccatctg gatgtggtga ccgcggattt
    24241 gcagaatgcg gtgatgaaga cccgaatgca gccgatcaag aaggtgttcg gacgctttcc
    24301 gcgggtggtc agggatctgg cgcgttcgct gggcaaagag atcaatctgg agatgatcgg
    24361 cgaagagacc gatctggata aaaacctggt cgaggcactg gccgatccat tgattcacat
    24421 ggtcagaaac tcggttgatc atggcatcga gatgcctgat gagcgggttg agtcgggcaa
    24481 atccagagcg ggtcgcatcg ttctttctgc agtgcaggag ggcgaccata tcattctgtc
    24541 gatcaccgac gatggccgcg gcatggaccc caacaaattg cgcgccatcg cggtcgcgcg
    24601 tggtgtgctt gacaaggatg cggccgaaag actttcggac acggaatgtt tcgatctgat
    24661 cttcgcgccg ggtttttcaa cgaagagtga gatctctgac atctctggcc gtggcgttgg
    24721 catggatgtg gtcaagaccc ggattggcca attgaatggc tcggttttca tcacctctga
    24781 gttgggtcgt ggaacgaaaa tagacatcaa ggttcccctg acattggcca tcatgccgac
    24841 gctgatggtg atgctgggag atcaggcctt tgccttccca ctggccaatg tcaacgagat
    24901 tttcatgctc gatctcagcg acaccaatgt cgtgaatggt caggagatca ttctggtcag
    24961 aaacaagccc ttgccgcttt ttcatctcgg tcgctggctg tgcaagaacc ctggagagac
    25021 acggaatgtc gagaatgcac atgtcgtgat cgtcactgtt ggaaatcagc gaatcgggct
    25081 tgtcgtcgat cagcttcttg gtcaagagga ggtggtcata aaatcattgg gtgcgatgtt
    25141 ggcgggcacg cagggtgtgg ccggagcgac cattaccgga gatggtcgaa ttgccctgat
    25201 tctggatatt cctggaatcc tgaagaagta tgcctgattt ccatgtctgg aatcattcag
    25261 ttcggaatcc ttgtgctgcg caaatggatt ctttctgaaa tgaacccaga gcgtgcatgt
    25321 tgagaattcc gcaagggcag gatgtcatgt cgattcgagt tctggttgtt gatgactcac
    25381 gatttttctg tcgcaggctg tgtgaaatat tgagcagcga cccggagatc gaagtggtcg
    25441 acaccgccat gaatggacag gaggcgatcg acaagaccat cgaattttcg cctgatctgg
    25501 tcaccatgga ttacgcgatg ccgatgatgg acggcatcca ggcggttcgt ggaatcatgg
    25561 agcggcgtcc gacgccgatc ctgatgctct ccagcatgac ctccgaaggc acgcaggtca
    25621 cgctggattc actggaggcc ggcgcggtcg atttcatcgc gaaggattcc atcgcgctgg
    25681 ccggccggga tggcgcgcag cgcgctgatt tgatcagccg cgtcaaaggc ctggccagca
    25741 gcaatttcag agcgaggcgc actgcccttg cccatggagt gggtacaagg agtgcaagac
    25801 cagttaccgc gcgaagcgcg acgaaaagaa tacgtctggt ggtgattggc gcatccaccg
    25861 gtggtcccgt tgcggttcag aaaatcctgc aggagctgcc ggcgactttt tcagcgccga
    25921 ttgtggtcgc catccacatg ccgcagacat ttaccggaat tttcgcggag cggctcaacc
    25981 agagttcaaa gctcaaggtc aaggaggccg ctgaaggcga tctattgcgc tctggagagg
    26041 tgctgctggc accgggcaac aaaatattga gcatctccgg tcagcagggt ggctgtatcc
    26101 atctgaacga accaggccgg aatgacattt actctccatc ggttgaccat gccttttcga
    26161 ctgccgcaca ggcctttccc ggctcggttc tgggagtcgt cttgactgga atgggtcagg
    26221 atggactgct gggcgcaaaa cagctgaaag agagccattc acgtctgtgg gcgcaggatc
    26281 aggccagtag cgtgatctat ggcatgccga tggcggtcag caaggccaat ctcgtcgatc
    26341 acgtctactc gcttgcagag ttgtccgaac atctcgttgc cgaggtgtgt tgatggatat
    26401 tctgagcatt ctcggcctca tcgtcggttt tggcggaatc attggcggca accttctcga
    26461 aggcggccac atcagtgcac tgctcaacct gccggcgatc atcattgtgg ccagtggcgc
    26521 cttcggtgct gtcatgctgc aaacaccgat gccaacgttt ctgcgtggac tgaagatgat
    26581 ggcgtgggtc ttcatgccac ctccggtccg catcgacgag gcaatccaga aaatcatcga
    26641 atggagccag attgcgcgaa aggaggggct gcttggtctg gaggccattg ccgaggatga
    26701 gccacacccc tttgcgaaaa agggactgca actcgtggtc gatggcggtg aacctgatga
    26761 catcagggag attcttgagg ttgagctgca tcagatcgaa gtgcgtgatc tggcctcggc
    26821 aaagatctat gaaggcttgg ggggctatac gccaacggtc gggatcattg gtgcagtact
    26881 gggtctgatc cacgtgatgg gcaacctggc cgatccaagc gcattgggtc caggcattgc
    26941 cgtggccttc gttgccacca tttatggggt ggcactggcc aacctcatat ttcttccggt
    27001 cggaaacaaa ttgaagagcg tggtccagtc gcaaagccgt tactacgaag tgatgatcga
    27061 agggttgctg tcgattgccg aaggtgaaaa tccgcgcatc atcgaactca agttgtccgg
    27121 ctatctaagc taggaaatct gatggccaag cgtcatgtcg agcatgaaca tgaaaaccac
    27181 gaacggtggc tggtctctta tgctgacttc attaccctgc tgtttgcatt cttcgtggtg
    27241 atgtatgcgg tctcttcggt caatgagggc aagatgaagg ttctctccga agcgatgacc
    27301 gaggaattca gaacgcggtc tgtgcaattt ccggtgccac ccacttcgtt gaaaccgatt
    27361 cagattggag agctgaaccg ggagcaaccg gcctcgatgg tggagggtca taaactttcc
    27421 ccgggcaatc agattgagca ggcgcagggc gatgaggccg aagaggagct ggaagggatc
    27481 aaaaaagagc tggaagtcaa acttggagag ttgattggtg aaaaccttct gaccatttcc
    27541 ggaaacggca gatggcttga gatcgagatc aactcgaaca ttctgttcga gagtggtgaa
    27601 gcggagccca ttggtgaggc caacacgatt ttcctcaagt tggcacagat attgggagaa
    27661 tatggcaacg cgatccgcgt ggagggtttc accgatgatc tgccgatcaa caccgccaga
    27721 tttccgtcga attgggaatt gtcggcggca cgggctgcca gaatcgtcac gattttgacg
    27781 aaatatggtg tcgatcccag gcgaatggca gcagtggggt atggtgagta tcaacccgtc
    27841 ggcgacaacc gcagcagtga agggcgcgcc aggaatcgtc gggtggtgct ggtgatttca
    27901 gcggatcgtc aggtgcgcaa acaacttgat ctgtcagtcg attctcctgg tcagtccagg
    27961 gatgtgatga agctggatgg cagtgagaag agcgaaactg tccagcgacc agaggtcatc
    28021 ggtagaaacc ctgccgtgtc ggcctcagga aactcgaact aacgaccttg tcggtacagg
    28081 ttttcatttt tggagagcgg tcatggagat catcgcgagt gcgaatcaga agggtggtgt
    28141 tggcaagacc acatccgtgg tcacgctggc gggcctgctg gcaaaaaatg gttccagagt
    28201 gttgatggtc gacatcgatc cgcagtgttc attgacctgc tattttggtt tcacacccga
    28261 actgctcgat gcgaccagct atcatctttt tcctgttccg ggcggcaata tcgatgccgt
    28321 tgacatgctg attcctctgg aggagaaaaa cctcgttctg ctgccatcgt cgccggccat
    28381 tgcaacagtt gaaaagcagc ttttcgatca gaagggcatg ggttttgtca tttccagcgc
    28441 gttgaaggga ttgagaagcc attttgacta cattttcatc gacacaccgc cggcactggg
    28501 cattctgatg atcaatgcac tggcggcgtg cgataaattg ctggttccgg tgcagaccga
    28561 atatcttgca ttgaaggggc tcgaacgaat gatcggctcg ctggagatga tggcgcggat
    28621 ccgcaaaaaa ccgctggact acacgatcgt tccgacaatg gtcgatcgtc gaacccaagc
    28681 ctcattgcgc agcttgcagg cacttcgtga caattatcga aggaacatct ggtccggctc
    28741 aattccagtt gacaccaagt ttcgcgaagc aagcctgctg ggccgaccgc ttgcgaatgc
    28801 ttttccatcg agccgcggcg ccaaggccta tgaagccctg tggcacgact tgaccaggac
    28861 acacccatct catgtcgaga gcgatgcgct ggagatggct tgaagttgat ctatattttt
    28921 agaaagccgg acgcggagtt tttccgttcc atccatatcg aatcaaaaat tgaatagagg
    28981 atacccggat gaatacagct gcagcacaga acgcctatga ccaggtcata caatgggtca
    29041 gcttcaagct ggatggcgag acctattgca tcgatgtgat gaaggttcag gaggtgctga
    29101 gatacactga aattgcccct gttcctggcg caccatccta tgttcttgga ataataaatc
    29161 tgcgcggaaa gatcgtgacg gtcattgata cccgggcacg tttcggattg aaagaggatg
    29221 agatcaccag caatacacga atcgtgatcg tcgagaccag ccagaagatc atcggcattc
    29281 tggtcgacag tgtttccgag gtggtctaca tacgtcagtc agagatcgaa acggcgccga
    29341 atgtgggaag catggacaat gcaaaattca ttcaaggtgt ctgcagcaag aatgatgagc
    29401 tctatattct tgtcgatctc gatcagcttc tcaatgaaga cgagtggctc gaggtggaaa
    29461 aggtctgatc tgttgctgcg gtcgaatggg gcggttgaaa tattgaggag atcgccatga
    29521 cggatctatc ggcacttgaa cagtcgtggc cggcgctgtt gctgtttttt tcaacgctcc
    29581 tggcctcctg tctgcttgcc agaatgcagt tcaacaggtt tgagaaaaaa atcacatcgg
    29641 atctgaaagt gcttgctcaa actgtagcgc tcattgagca gagcagtttt ggttttggga
    29701 gaaatctgca tctgatcgaa cgcaatgtca aagagctcag gatgctgcat gatgaacttg
    29761 agtcgaagag tggcgccgag gatctctatc tgcaggcagc ccaagtggcg gaaatgggcg
    29821 caacggctgc tgagctgatg aataacttcg gtttgtccaa ggtggaagcc aaactgatcg
    29881 aggcacttca cgccgcgcgc gctgacgcca ggcttcattg acctcacgga gtccttcttg
    29941 attcaacacc ggcccatggc cggtgttttt tattccagcg ttttcacccg ttgcgcttct
    30001 tcgatgaccc ggtgattttc tgctcaggtg ttactttgag aaggagcact tgtgagaggt
    30061 gtggttcgga gggtttgaaa agcgggcccc tgtcggaact ttcggaaatc gatctgaccg
    30121 tatgatcgct gtcgtttcac ggtccgccgg aatcagcgaa cgcaggcagc acatggctgc
    30181 cggccggttg ccagcgaaag tgcaggccac ccgctccggc tgcactgcgc cagcccggca
    30241 tgatggcaac gccgggaatg gcgaccaggc tgtggtcctg atagatcaac ggaatatggc
    30301 tgcgcagcca aggaggcacc cctgcctgtt gcagcagcct cttgaggcga tgccggtcag
    30361 cctgctgcgc ggggtcacca ctgtgccgaa agcgaagggt gatggggatg ccggggcgca
    30421 gtgccacgcc gcctgcatcc tgccactgct gccagagccg gctgccgtcg ggcagttgca
    30481 ggaacgctgt gctcagatcc cactccagtt ccattgtgct ggccggcggc ggcaggggcg
    30541 gcatcgcgta gagaaaatca cggtagcggc gcacttcgac ccctggccag ttcaccagcg
    30601 gctggcggtc ggcggcggcg ggcatcactt cgtccaggat gcgttgcagg cggttgtggt
    30661 cgggcagcgg caatccattc agccccagcc agtggcgcag caggttgcgc tggcgcgcat
    30721 cgtcgagctg ttgcagtgag gggatgtgca gacgatcgag acggtgttgg ggtgcatttg
    30781 agaagtcgat cgcggccagt tgttgcagca gttgtgcgct ttcggcggcc agcgcggcac
    30841 ttcgcagcag ggtccgggca tggttgggcc agcgggcggc gatggcaggc cgcagcgtgt
    30901 ggcgcaggta gttgcggtcg aatgcggggt cgaggttgga gggatcttcg atccaggtca
    30961 gctggaccgc atcggcatag gcgcgcagtt gcgtgcgggg cagggcgagc agtgggcggg
    31021 ccaggctggc gtggccgagt ggacgttggg caggaatgcc ggccactccg gccggaccgc
    31081 tgccgcgcag cagtcgcagc aacagggttt cgatctggtc atcctggtgg tgggccatca
    31141 gcagttggtc gccgggcagc aggaagtcgc tgaaggcgcg gtagcgcacc tcgcgcgcgg
    31201 cggcttcgac gccgcggcca cctgatctgt ccaccgcgac gcagcgcagg tgaaagggca
    31261 cctcgagctg ttgtgccagc gctcgacaat ggctggccca agtggccgcc tcggggtgca
    31321 gatcgtgctg gacatgcagt gcatgcagcg gttgcgtggg cagggcatgc agccggcgct
    31381 gtgccagaca gtggacggtg gcgtggagca gcaccatcga gtcgagtccg ccactgaagg
    31441 cgaccaccca gcgtgccggg gcgggttcgg ccgccagctg gcgggagagg gcggtgacga
    31501 ccggggagtc gctcactgcc ggccggcctc agttcacata gtggccgtag gagaggtagc
    31561 ggcggtagcg gcgttcgacc agctcctcgg ctgaatgacg tttgaactcg gcgagccgtt
    31621 cgatcagcgc atgcttcatc gcggcggcgg tggcgtcata gtcgcggtgg gcaccgccca
    31681 gtggttcggg aatgacctgg tcgaccagac ccagctcttt cagtcgtggc gcaatcatcg
    31741 ccatcagttc ggccgcttcg gcggcctttt cgttgctttt ccacagaatg gtcgagcagc
    31801 cttcgggcga gatgactgag taggtggcat attgcagcat catcagatgg tcgcagacac
    31861 cgagcgccag cgcgccaccc gagccccctt cgccgatcac ggtggccaga atcggcgtct
    31921 tcagccgtga catcactgcc aggttgcggg cgattgcctc gctctgtccg cgctcttcag
    31981 actcgatgcc cgggtaggcg ccgggggtgt cgatcagtgt gatgatcgga atcgaaaagc
    32041 gttcggccag ctccatcagc cggcacgcct tgcggtagcc ctcgggctgc ggcatgccga
    32101 agttgcgcag gatcttctct ttggtgccgc ggcccttttc gtgggcgatc accatcaccg
    32161 gttcgccatc cagccgcgcc agaccgccga tgatcgaggg gtcgtcgcga aagtggcggt
    32221 cgccatgcag ctcatcgaat tcgtcaaaga tgcggttgat gtagtcgagg gcgtagggtc
    32281 gcagtggatg gcgcgagatc tgcacgatct gccaggccga gaggttgcca aagatcgatt
    32341 cggtcagctt gcggctcttc tcttcgagct tggcgatctc gtcgctgatg ttgagctcgt
    32401 tgtcgctgcc gaccatgcgc agctcttcga tcttggcgcg caattcggcg atgggctgtt
    32461 cgaaatcgag gtggttgagg tgcatgttcc ggcggttccg aagggttgat gcggtcggca
    32521 ggcaagagcg ccgaaggtcc gcaaaaaggg ctcaatgata actcattctg acccgatcga
    32581 tgccgaactg gccgcgcagc cgttcgagca gctgatcggt cggacgcacc cgccaggcag
    32641 cgcccagatg gatgcgccca cccactgcat cgccgcggta gttgagcagc accgggcagc
    32701 cgtcgccgcg gtagggggcg agcagttcgg cgagctggcg gtcgaagtcg ttcggcaacg
    32761 catcgccccg cagatcgagt tcgagctggc gaaccaggcg tgttcgtgcc tcgtcgaggg
    32821 tgctgaggct cttggcgcgc gccttcaggc tgctgttgta ctcatcgagg ctgacgctgg
    32881 cctcgatcag cagaatctca tccttgatca gtttgtcgcg ctgctgctga tagtcatcgg
    32941 caaagagcga gatctcgatg cgggcggtgc ggtcatccag tgtcagaaag gccatggtgt
    33001 cgccgcgctt gctgcgcagc gtgcgcacac ccagcagcag tccggcgaag agctgcggac
    33061 cgttgtcggg ctgcaggtcg gcgatctggg tcaccttcat cgcccgcagt tcgtcacggt
    33121 actgttcgat cggatggccg gaaagataga ggccgagggt ctccttctcg gcctgcaact
    33181 gctcgcgcag gctccagcgc tgcgcgctga cctgcatgac cggttgactg ctgatcaggc
    33241 tgcccggcag cagttcgccg aacagatcgc gggtgccgct ggcactgttg gcggcgctct
    33301 gctcggccgc ctgcagggca ttgggcaggg tggcgagcag ggtggcgcgg tcgctgccca
    33361 gctcatccat ggcgcccgat ttgatcagcg cttcgagcgc tcgcttgttg agcttgtggc
    33421 tgtcgcagcg gcggcagaag tcgaacaggt cgatgaagcg gccgccgctg cggcgggcac
    33481 tgttcaatgc gtcgatcggc ccctcgccca gccccttgat ggcgccgagc ccgtagatga
    33541 tcgacccctc ctcgacatcg acactgaagc ggaattcgcc gcggttgacg ttgggtggtg
    33601 acacccgcag ccccatcttg cggcactcct ccaccagcag caccaccttg tcggtgtgct
    33661 gcatgtccgc tgacagcacg gcagccatga agaaggccgg gtagtggtgc ttgagccagg
    33721 cggtctggta ggagatcagc gcgtaggcgg ccgagtgcga cttgttgaaa ccatagccgg
    33781 cgaacttctc catcaggtcg aagatctcct cagccaccgc cggcggcacg ccacgctggc
    33841 tggccccggc agtgaagctc ttgcgctgat cggccatctc ttcggccttc ttcttgccca
    33901 tcgcccgccg cagcatgtcg gcgctgccca gggtatagcc ggccagcacc tgggcgatct
    33961 gcatcacctg ctcctgatag aggatcaccc catgggtgtt ggcgagaatc ggcgccaggg
    34021 ttggatgcag gtagcgcacg gcggcccggc catgcttgcg attgatgaag tcatcgacca
    34081 tgcccgattg cagcggtccg ggccggtaga gcgcaaccag cgcgatgatg tcctcgaagc
    34141 agttgggcag caggcggcgg atcagctcct tcatgccgcg cgattcgagc tggaacaccg
    34201 ccgtggtctc gccaccttgc aggtagcgga acacctccgg gtcgtcgagc ggcagacttt
    34261 cgatcgcgat cggcggttgg ccctgactgg cgcgagcctg attgatcgcc ttcactgccc
    34321 agtcgatgat ggtcagcgtg cgcagaccga gaaagtcgaa cttgaccaga cccgccgcct
    34381 cgacatcatc cttgtcgaac tgggtcacca ggctgccgcc cgactcatcg acatagagcg
    34441 gcgtgaagtc ggtcagccgg cctggcgcga tcaccacgcc gccagcatgt ttgccgacat
    34501 tgcgggtgat gccttcgagc ttgaacgaca tctcgatgat ctcggccacc tcgtcgttct
    34561 gcgccatcaa ctccttcagc gccggctcct gtttcagcgc cttctccagc gtcatgccga
    34621 cctcgaacgg gatcagcttg gagatgcgat cacccaggct gtagggcttg ccctgcaccc
    34681 gggcgacatc acgcaccacc gccttggccg ccatggtacc gaaggtgatg atctgcgaga
    34741 ccgcctcgcg gccgtagcgt tcggagacat agtggatcac ccggtcgcgg ccatccatgc
    34801 agaaatcgat gtcgaaatcg ggcatcgaca cccgctccgg attcagaaag cgctcgaaca
    34861 gcagctcgta gccgatcggg tcgaggtcgg tgatccgcaa tgcataggcg acgatcgaac
    34921 cggcgcccga gccgcggccg gggccgaccg gcacgccgtt gtcgcgcgcc cagcggatga
    34981 aatccatcac gatcagaaag tagccggaaa aacccatctg cacgatcaca tccagctcaa
    35041 attcaaggcg ttgcagatag ctcttgcgct tttcttcatc atcgatcgac tgctgggcga
    35101 accgctgcat cagcccctca tgggccagcc ggcgaaagaa ggcctcttcg ctttcgtcgg
    35161 tcggcactgg atagtccggc agaaagttct tgcccaactg caactcgagc gtgcagcggc
    35221 gggcgatctc gacgctgttc tccagcgcct cgggcaggtc gtggaacagc tcggccatct
    35281 cctgcgggct gcgcagatac tgcgcctcgc tgtagagccg gggacggcgc ggatcgtcga
    35341 gcgtgcgccc ctcctgaatg cagacccgcg cctcatgggc ctcgaaatcg tcccggtcga
    35401 gaaagcagac ttcgttggtc gccaccaccg gacaggcgag ccgctgcgcc agcgccaccg
    35461 cccgttgcag gtagagctct tcgtccgctc ggccggtgcg ggtcagctca agatagaagc
    35521 ggtcagcaaa ggcctgttgc cacgccttca gtcgtgcttc ggcctgttcc ggccggtcgt
    35581 tcagcagcag ccggccaatc tcgccatcac ggccacccga cagggcgatc agcccgggcg
    35641 cggcctccat gacccactcc tgcctgacca gcgcgcgccc ctgatggcgg ttttccagat
    35701 agccacgcga gatcagcgtg gtcaggttgc ggtagccggt ctggtcacgc accagtaaca
    35761 gcagcggcgc gaactgcccg ggctgttccg ggttttcgac cagcagttgc gcgccgacga
    35821 tcggtttgat gccggcggaa cgggctgccc gatagaactt caccagcgcg aacagattgc
    35881 tgatgtcggt gatggccacg gccggcatcg ccagctcgcg cgtccgttcg accagggcat
    35941 cgatggtgat caggccatcg accagcgaat attcggagtg cagatgcaga tggacaaagc
    36001 cgggggcggt gctgttcatg ccgtgaaccg cgcggttgcg tcatgccgga aaggcgagac
    36061 agcagaggaa taaaaacagc ctggagtcac cggttggatc tcgcggaggg tggacagcgg
    36121 gtttgctttg gcgcaaggcg cagtgtgtag tgcgacctgg gctgattttc tccgatcacg
    36181 gcatcctcga ccagcgacag gatctcgatg gctggcggtc agaagtgcag ctgtacgctc
    36241 ccagtcgctt tcaatatcaa tccccccggt gatgcgataa aatcgcccgt tccatgacga
    36301 atggccaatt catcaataac ttctggagca tgggcatgcg tttgatcctg ttgggtcctc
    36361 cgggcgcggg caaggggact caggcccgtt tcatctgcga gcatttctcg attccgcaaa
    36421 tctcgaccgg cgacatgctg cgtgcggcgg tgaaggccgg cacgcccttg gggcagcagg
    36481 tcaaggcggt gatggattcg ggcgccctgg tcaccgatga gatcatcatt gcgctggtga
    36541 aggagcgcat cgaacagcca gattgccaga agggctttct gtttgatggc tttccgcgca
    36601 ccattcccca ggccaatgcg atggtcgatg ccggcgtgca gatcgaccat gtggtcgaga
    36661 tcgcagccga cgacgaggag atcgtgcggc gcatcagtgg ccgtcgtgtg catgcgcaat
    36721 cgggccgcgt ctaccacgtg ctgttcaacc ggccgcaacg cgaaggcctc gatgatgtga
    36781 ccggtgagcc gctgatccag cgcgatgatg acaaggaaga gaccgttcgc aatcgactga
    36841 agatctacca cgagcagacc cggccgctgg tcgatttcta ccggaaacgg gcggagcaat
    36901 ccgcctcgct gcactatcac accatcgatg gcatgggtga ggtcgatcag gtgcgtgcag
    36961 cgattctggg tgtgcttggc tgaggcggtc cgcccgcacg gccgatttca cgctcgacca
    37021 cttcgttcca tgccgcaacc aacctcagtg gcgcagtcgc cgcgtcgata tggcgttctg
    37081 ctggtcaatc tcggttcgcc agccgcgccc gatgccgccg ctgtgcgccg ctttctgcgc
    37141 ccgttcctgg gcgatcggcg ggtggtcgag attcctcgac cactgtggtg gccgttgctc
    37201 aatctgttca ttctgccgtt tcgcgcgccc agggtggcca ggctctatca gcgcatctgg
    37261 atggccgagg gtgcgccgct gcaagtccat ctcgagcggt tgcgagaggc cgttacggcc
    37321 cggtttcatg cagggagcag cggaggagat cggatcgagg tgcgtgcggc agtgacctat
    37381 ggtgcgcctg cgattgccga tcagctcgat gcactgatgg cagccggcag tgatgaggtc
    37441 atcgtcattc cgctctatcc gcaatattcc gccaccacca ccgcggcggt gttcgatgcg
    37501 gtgtcacgct ggatgcagca gcgccgtgat ctgcccggcc tgaccttggt caaggattac
    37561 catgaccatc cgcgttacat cgaggcgctg gcgcaatcgg tgcggacagc gcagcagcag
    37621 ttcggcacac ccgagcggct gctgttctcc tttcatggca ttccgcaagc caatgttgac
    37681 cagggtgatc cttacgcacg gcactgccac cgaacggctg aagcagtggc gcaggcgctg
    37741 ggactggagc tggggcagtg gctggtggcg ttccagtcac gcttcgggcc ggcgcagtgg
    37801 ctgcaaccct acatggatga agcagtgaag gcgctggccg cagagggtgt ccggcacctg
    37861 caggtgattt cgccgtcatt ctcgctcgac tgtctggaga cgctcgatga gatcgaccat
    37921 gaatatcggc agctttttct ttcgcagggt ggcgagcggt tccactacgt tccggcgctc
    37981 aacgccggtg attctcaggt cgagctgctg gtcgagctga tctcatgcca tctgccggtg
    38041 gcacgctgag gcggccgatg ggcgctgctc tggggtcagg caatggccag acaggcgtgg
    38101 acgaagctgt agatggccag ataattgttt cggctcaagg tgtcgagttc gatgccgtag
    38161 ccgattttgc gttcagcgtc agcagttgaa gtgctcttgc ctggttcgct catcgagcga
    38221 atggtgccgg ccggtgtcat cgcatagcgg tgctcgtcga tctgcagctc catttcgatg
    38281 tgtatcggct gtcccacctg caaggcgttg ggcgtcgtct ccagccgcat tccgcgggtg
    38341 ctgatgtcca gaatgtgtgc cggcaatcgc gcttcgtctg cttctcccag attggtgatc
    38401 actgtcgcca tcagatcgca gggggcgcgc agggccgagc gcaccttttc gatggcgaca
    38461 tcggccgggt aactcagatg cagataggga aaggggtagg tg
//
LOCUS       DAORCQ010000012        35634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_615_length_35634_cov_5.820006, whole genome shotgun sequence.
ACCESSION   DAORCQ010000012 DAORCQ010000000
VERSION     DAORCQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 35634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35634
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_615_length_35634_cov_5.820006"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKI23_02195"
     CDS             <1..591
                     /locus_tag="PKI23_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNN35781.1"
                     /translation="FKFTTSNKQFNFLQHIMTILDTHGRAGVVLPDNVLFEAGRAGED
                     IRRRLLEGFNFHTLLRLPTGIWYSPGVKANVLFFDKRPAAREAQTRELWVYDYRTNVH
                     KTLKTKRLERGDFDDFIRCFHARQETERFRRFSYAELAQRDKLNLDIFWLKDDSLEDL
                     GSLPEPDVLAAEIVENLEAALTQFRSVSLELAGEEG"
     gene            complement(634..1530)
                     /locus_tag="PKI23_02200"
     CDS             complement(634..1530)
                     /locus_tag="PKI23_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN35782.1"
                     /translation="MGKRTVFSIVILLLAVAADARPRDGLDDPQTPPDRHGKSAARTI
                     AYGRDALQRLDVWTVPGSDAAPLVMFVHGGGWQRGSKDNASSRWLPAHLTQQGYAYAS
                     IDYRLVPAVAVEQQAEDVAHALRALLDRAAELGIDRRRVVLIGHSAGAHLVALVGTDE
                     RHLKSAGLGFADLRGVIANDGAAYDVPAQLQEDAPMMHQTYLEVFGSDPARQWALSPT
                     HQAAAPNAPAFLLLHVQRPDAVRQTEALGRALAAAGSAVDYGSFAGTGLRGHMEINRR
                     LGDPDYPATALVDGWLRKNLAE"
     gene            complement(1576..2025)
                     /gene="fliJ"
                     /locus_tag="PKI23_02205"
     CDS             complement(1576..2025)
                     /gene="fliJ"
                     /locus_tag="PKI23_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNN35783.1"
                     /translation="MINSKAIWPVVKLAEEREQTALLALNQAAGQLREEQQRFDQLRG
                     YLDDYLSSTPGDYPLPGAAFMLQNRQGFVDRLKVAIGQQERRLLIVRNDHAALEAGWR
                     ELRLRRLGLQQLAEEADRQARLRANKKEQHRLDEMATQRVIANRVQR"
     gene            complement(2022..3371)
                     /gene="fliI"
                     /locus_tag="PKI23_02210"
     CDS             complement(2022..3371)
                     /gene="fliI"
                     /locus_tag="PKI23_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNN35784.1"
                     /translation="MTRPQLLERIARYPLDTASPPRPLVSGRLVRSVGLTLEVVGLGA
                     ALGECCLIESANGQHIEAEVVGFDEGKLFLMPMRRIGGLKPGARVLPGGDEARIEVGD
                     ALLGRVIDGLGLPLDGLPPPQCSHTIPFAGPMINPLRRLPVREPLDVGIRAINGLLTV
                     GRGQRMGLFAGSGVGKSVLLGMMTRFTTAQIVVVGLVGERGREVREFIEHNLGAEGLA
                     RAVVVAAPADESPVMRLRAARLATRIAEHFRDQGHDVLLLLDSLTRFAQAQREVALAI
                     GEPPATRGYPPSVFAQLPELVERAGNGDQGGGSITAFYTVLMEGDDDQDPVADSARAI
                     LDGHVLLARRLAEEGHYPAIDIEASISRVQPQVVSAGQLAQAQRFKQLYSAYQRSREL
                     IQIGAYQRGADADTDLAIQLIPQLRGYLRQGLNESFSLASSSGELEQLFNTAETAGR"
     gene            complement(3368..4051)
                     /locus_tag="PKI23_02215"
     CDS             complement(3368..4051)
                     /locus_tag="PKI23_02215"
                     /inference="COORDINATES: protein motif:HMM:NF018358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNN35785.1"
                     /translation="MTKSLLLDVESVRKLYRWAPPQISSSGEVIRSPDAARQAEVEEQ
                     VALRIAALKAAAHTEGHAAGYALGREEGERAGREIWQNEAARLTRLLTHLADPLAERE
                     AEIETALLNLVIEISRALLEHELKSSGETVLSVVRQAIAALPKGAEQVSLLLHPDEQA
                     LLLKLPQKLPLTIEADPGVSRGGCRIESRDSCIDFTTEYRFRELVRNLLGRELSAAQR
                     FIDPAVDST"
     gene            complement(4051..5064)
                     /gene="fliG"
                     /locus_tag="PKI23_02220"
     CDS             complement(4051..5064)
                     /gene="fliG"
                     /locus_tag="PKI23_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNN35786.1"
                     /translation="MSERGNSSLRPIQRAAILLMTIGEENAAAVLKNMGPKEVQRVGA
                     AMAELNNISRSDVEQAVYGFVDEAGDLTGLGVGNDQYIRKMLVSALGEDKAKSLADRI
                     LIGGNTSGLDTLKWMDARSVAGMIRYEHPQIQAIVIAYLDGDHAAEVLVNFPPQVRLD
                     IMMRVARLDTVQPEALQELNRIFERQFSGGAGTQSATLGGVKTAAEIINNLDSSVAAQ
                     VMEGIREENEEMSNNIQDRMFVFDNLIGIADSGIQALLREISSENLILALKGADEALK
                     QKIFGNMSKRAGDMLRDDLEAKGPVRVSEVEAAQKEILAVARRLADAGTIILSAGGGE
                     EML"
     gene            complement(5057..6775)
                     /gene="fliF"
                     /locus_tag="PKI23_02225"
     CDS             complement(5057..6775)
                     /gene="fliF"
                     /locus_tag="PKI23_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNN35787.1"
                     /translation="MGLLSGMVNLPLLRQFGLLVGLAASVAIGVAVALWSREEPYMPL
                     DAALGGADAARVVSLLDAQKIPYHIDPRSGALLVPADKVQIVKMKMAGAGLGGERAQG
                     YELLDQESPFGSSQFMEATRLQRSLEGELARTITGMYAVRSARVHLALPKSTTFVRGG
                     NKPRASIFVELQSGNALTPDQVSAMVNLVTSSVADLDAKDVSVVDQKGRLLSRNEEEG
                     ALADAGKQLEHVRRIEESLIGRVRRVVEPLVGYDHFTAEISADVDFTEQEQTSEQYQP
                     DPPALRSEQTLVERMMGGAGDRGGVPGALSNQPPGAVSVPEVAARPTTPPLPAEAAAG
                     AAASSSAETGRSRNQATRNYELDRTLSHTRQQVGKVRRLSIAVVVDDLPDLAPPKKGS
                     AKKGAAEGKEAANTVPRRAWSDEELRKLTELVKGATGYDAARGDTLSVVNSSFVKREI
                     EPAGEPVAFWSEPWFWSLAKQLLGGLFVVLIAIAILRPVMKNISAFSGTRPATLVAYP
                     AGTVVMGEGGEMMQTAAPITEPGRMISFDGSKSYEQQLDAVKGLVQQDPGRVAQVVKK
                     WVNDGE"
     gene            complement(6882..7256)
                     /gene="fliE"
                     /locus_tag="PKI23_02230"
     CDS             complement(6882..7256)
                     /gene="fliE"
                     /locus_tag="PKI23_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNN35788.1"
                     /translation="MVNRINDRSDIQQLLQQMRSIKAEMNSQRSDVGSVPGMEMAGIG
                     APAVTSSSGFGDLLRQAVDGVNDMQSKSAQLAQAYELGQPGVDITQVMVAMQKSSLSF
                     QALTQVRNKVVDAYQTIMNMPI"
     gene            complement(7544..8905)
                     /locus_tag="PKI23_02235"
     CDS             complement(7544..8905)
                     /locus_tag="PKI23_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNN35789.1"
                     /translation="MMNRTTILVIEDDLDLAEALTETLELAGFDACSVNSGEAALARL
                     EQIEPGLLLCDVNMPGMDGHQLLQQVKGSRPMLPVVLMTAYGSIEKAVQAMRQDAADY
                     LVKPFSSERLIETVRRHLAAVPVDEHDPLAEAASSRRLLALAQRVAQSDSNVLICGES
                     GTGKEELARFIHRHSPRASQPFVAINCAAIPENMLEATLFGHEKGAFTGAYQSQPGKF
                     EQANGGTLLLDEISEMALPLQAKILRVLQEREVERIGGRRTLQLDLRILATTNRLLHQ
                     EVEQGRFREDLYYRLSVFPLEWLPLRERHEDILPLARRLLARHAQRAQRPAPLLTPAA
                     ERALLAHPWPGNVRELDNVLQRALILQEGCEIDLSQLHLGPLTGTASHSVRVAISHSE
                     PVTAATDLGSDVKSHEHRLIMETLRRCQGSRRDSAQQLGISERTLRYKLAQMREAGLA
                     LEV"
     gene            complement(8950..10134)
                     /locus_tag="PKI23_02240"
     CDS             complement(8950..10134)
                     /locus_tag="PKI23_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN35790.1"
                     /translation="MLHPVPFAPQTTLHWPESVGLSAHPPAASGAYRALSERVNQLRD
                     ELASESAERRHELAQKERLADRHEALLRLLPGGVVVLDERGIVTECNPAAIELLGEPL
                     LGARWSTVIERCFAPQAGDGHEVSLKDGRLIALATRSLDGGHGQLILLTDQSETRALQ
                     RRLHRDERLVSLGKMMAALAHQLRTPLAAAMLYSDQLRSERLSAERRDRAVQRLTERL
                     QHMEEQIRQMTVFIKGEVTLTEQITLAQLLEQIERSAEVAVTAAGARIDYLLEAPPAL
                     LRCNQAVLVGAVVNLINNALQAATAQPRLRVAVTRLDAERIAIRVDDNGPGLHGREQA
                     ALGEFYTTKSSGTGLGLPVVLAVARAHHGDFLLTDLPGLQGRDPGGARAELRLPVVQW
                     LT"
     gene            complement(10285..11787)
                     /locus_tag="PKI23_02245"
     CDS             complement(10285..11787)
                     /locus_tag="PKI23_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017477453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNN35791.1"
                     /translation="MRNEQWLLVIEDEPARQAELRIITDFLGEAAVVVASHGWREQLK
                     AAPQAEGPPVAVLLGSRAEGGSLPELLTQLHQWDGCVAVIDISGQAADESVPNELKRM
                     VIAREAWPLGYNRLIDHLHRARVYRDQHERARGESAPRRREIHLFRSLVGTSRAIQKV
                     REMMNHVADRDATVLITGESGTGKEVVARNLHYHSPRRDKPFVPINCGAIPPELLESE
                     LFGHEKGAFTGAITARAGRFEMAHGGTILLDEIGDMPLNMQVKILRVLQERTIERVGG
                     SRPIEIDVRVIAATHKDLEAMIERGEFREDLYYRLNVFPIEMPALRERREDISLLLQE
                     LIARIEHEERGAPRFSSAAIMSLCRHDWPGNVRELANLIERMAIMHPYGVIGVHDLPK
                     KYRYDDSEVETFLPEGEPRPIPQALPTMAQPLSTPPQQQDEALPRDLKLPLNGVDLRE
                     YISHLETTLIQQSLDYNNGVVARAAEQLHLRRTTLVEKMRKYGMQRKEWE"
     gene            complement(11919..12686)
                     /gene="kdsB"
                     /locus_tag="PKI23_02250"
     CDS             complement(11919..12686)
                     /gene="kdsB"
                     /locus_tag="PKI23_02250"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNN35792.1"
                     /translation="MDFVVVIPARHASSRLPGKPLALVGGLPMVQRVHQQARRSQARE
                     VVIATDDLRIAEVARGFGAEVVMTSTEHASGTDRLEEVVRLRRYAPEQIVVNLQGDEP
                     LIPPQLLDQVAAALLHGTAPMATLCEPIVEREDFFNPNVVKVVRDERGHALYFSRAPI
                     PYPRGIALGSDVAWQQAGVPALRHIGLYAYRAGLLHRFVRWPIGTLERIEALEQLRLL
                     SQGIDIRVELAQVTPPPGVDTPEDLERINRLWQMRMP"
     gene            complement(12690..13727)
                     /gene="lpxK"
                     /locus_tag="PKI23_02255"
     CDS             complement(12690..13727)
                     /gene="lpxK"
                     /locus_tag="PKI23_02255"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNN35793.1"
                     /translation="MTLRGRIEARLLHAWYGGGPPLPGSRVLAWLYGRLLALREWLYR
                     HGILSARQVGCPVLVVGNLTVGGTGKTPLVIALAEQLQAWGYRPGILSRGYGAAPSSL
                     PHRVESEEDATRCGDEPLLLARRTGLPVMIDPDRVRAARALLAAEEVDLLLCDDGLQH
                     RRLAREIEIVVIDGQRRFGNGLLLPAGPLRSPLDRLDSVDYRVVNGAAGSESDGAVAM
                     RLKPQHLVNLATGEEIEVTAWQGERRVHALAGIGHPQRFFATLRELGFDPIEHPFADH
                     HPFVAADLPADGHPLIMTEKDAVKCAAFARPHHWWLRVEAELPERFWQALHVQLAQLA
                     QPCGATPPKEQ"
     gene            complement(13724..15508)
                     /gene="msbA"
                     /locus_tag="PKI23_02260"
     CDS             complement(13724..15508)
                     /gene="msbA"
                     /locus_tag="PKI23_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNN35794.1"
                     /translation="MNRTPDATEVARTDWMVYRRLLGHAYPYWKFLLLALFGYLLMSG
                     ASIGAAELMKTIIDSLSQKQPGYRAFIPLAMVALIGGRGLGSFVGNYSMAYVSNALVF
                     DLRQLVFGKFMTLPAVYYDTHAGGHLISMITFQVTQVTDAITKAGKIMLEHGLFLIGL
                     VAYLLWMNWKLSLLLLVVAPPIFFIVRYAGRRFKRISRRIQHSMGDVTHVTGEMINGY
                     REVRIFGGEASEQARFQRVSNFNRQQLMKLTATEASNGPAVQLLVSLAMALLMWLALD
                     PTLVDQLSPGGFVAYITAASLLARPAKMLADVQSIIQRGLAAADEIFHMLDQPSEPDG
                     GTLSIERARGDIDYRDVSFGYHGGELPVLKQISFSIPAGKMVALVGRSGSGKSTIVSL
                     LPRFYDHQSGQITLDGIEIGQLRLASLRAQIAYVSQQITLFNDSVRNNIAYGATAGRS
                     EVQIREAARRAHALEFIEKLPDGFDTLVGDDGVMLSGGQRQRIAIARAFLKDAPILIL
                     DEATSALDTESEQKIQAALEEVMKNRTTLVIAHRLSTIIHADQILVIEGGELLESGSH
                     AELLARNGAYTALYERQFADEAAPVVTP"
     gene            complement(15587..16012)
                     /locus_tag="PKI23_02265"
     CDS             complement(15587..16012)
                     /locus_tag="PKI23_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNN35795.1"
                     /translation="MKFRRRREDPIDTAINVTSLIDVMFVLLLFFMVSTTFTKQSNLK
                     VDLPEAGGAQTPVDEKRIEITISASGEYRLNALPLPNASLDALKAALIAQVGSDRNLP
                     FVITADRQTPHHAVVTAMDAAAQLGFARLSITTQQPAGQ"
     gene            complement(16067..16681)
                     /locus_tag="PKI23_02270"
     CDS             complement(16067..16681)
                     /locus_tag="PKI23_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNN35796.1"
                     /translation="MLEIIVAGGWVMLPILLCSVIALAIGFERAWALNRRRILPDNLL
                     IQVWGWIKNNQLDSDKLKKLKEESPLGRILAVGLANSKYGRNIMKECIEEEAGHVIHE
                     LERYLDTLGIVAAISPLLGLLGTVFGMIKIFAVMMGQGATNVSSLAGGISEALITTAA
                     GLSVAIPALVLHRYFLRRVDNLVVELEQEARKLVEVLHSERDLG"
     gene            complement(16685..19102)
                     /locus_tag="PKI23_02275"
     CDS             complement(16685..19102)
                     /locus_tag="PKI23_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNN35797.1"
                     /translation="MVARLLALCGGIALVALLPELPDRTLLASCVVLLALLRCKYRAV
                     APALAFFIGLSWATGYGCLGLASRLPAEFEGVDLRVEGVVGSLPQWRTGAAQRPEALF
                     LFDVERWLPSEPDGEVPAALERVGRLRLSWRDAPPVAPGEKWQLRLRLKRPHGSVNPG
                     GFDYEAWLFRQGIDATGYVRSGDDNLRSAPAGWHAWDSQLRAWVDGLLVPQLHERRHA
                     GLMRALIIGESSQLTPQEWRLFGATGTIHLMVISGLHIALVAWCCHQLGGWVGRHLLA
                     PGLLWSAGSVAAWSALLGAFLYTALSGFALAATRALIMTAIWAAAKLLRRQIDPWFGL
                     VLALSLVLLSDPLAPSSSGFWLSFGAVMTLMLLFQGTQQQEWSRLRRFGREWWLAQWG
                     LFFAMLPILLWWNYSASLASPIANLLVVPLFDLLLVPLLLLATLLTPLSSTLADLLFA
                     GGDWLLDLTDRFLHWMAASPLAAARVIQPPAWLLWLLLPALLLWLLPRTLPGRPWALL
                     LLLPLWLMPVRHTDSEPLRLTVLDVGQGLAVVVEVGERLLIYDTGPHASERFDAGSQI
                     IAPYLRWRGRTVIDALVVSHADRDHDGGAAGLLREVPVGRVYVSGDSLWGLQRDQIDL
                     HPCRSGDGWRWQEVEFRFLHPDLPRFGSDNNNSCVLQIKSGDQSILLTGDIEAAAERQ
                     LLQRWGSALQATLLVAPHHGSNSSSMPDFIDAVAAKQVIFSAGYRNSYRHPHPQVVQR
                     YAETGATLYNSAATGAVGYAIQSGGGMAPPSLARQQARRYWYDRLSASAADAALMPHE
                     SDSRKEK"
     gene            19179..19775
                     /locus_tag="PKI23_02280"
     CDS             19179..19775
                     /locus_tag="PKI23_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNN35798.1"
                     /translation="MPRRIFKRIAPDPHKIREQRALRFLGETIHHPALWHFSRRSVAT
                     AFAIGIFCAILPMPGQMVLATLLALWLRCNIPIAAGLAWATNPITAPPILYVTYRTGC
                     WLLGQEPQIDHIEVSFEWLKEFALSAWEPLWVGSVVVGLLLGAASYLAIRMVWRWHIT
                     RKWQRRTPTRPALNPVAAAVPLPAAIPDPSARQMPGHE"
     gene            complement(19696..20391)
                     /locus_tag="PKI23_02285"
     CDS             complement(19696..20391)
                     /locus_tag="PKI23_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN35799.1"
                     /translation="MSSRSAQPVLQGSGLIKTYHQGPEEVQVLRGVDFAAWRGERVAV
                     VGSSGSGKTTLLNLLGGIDHPTAGQVRLAGIDLKTLDERALCRFRNEQIGFVYQFHHL
                     LPEFTALENVALPLLLNRHAVALAREQATQMLQQVGLGHRLQHKPSELSGGERQRVAI
                     ARALVNGPALVLMDEPTGNLDSRTSNEIQELMARLSEGGDTCFVVITHDQAFAERMDR
                     VLRLEAGRLLPQD"
     gene            complement(20384..21628)
                     /locus_tag="PKI23_02290"
     CDS             complement(20384..21628)
                     /locus_tag="PKI23_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNN35800.1"
                     /translation="MFRPLPLFIGLRYTRAKRRNHFISFISLVSAIGLTLGVTVMIVV
                     LSVMNGFHRELRDRILGMVPHATVFPEQGALADWQSLGAQLQKHPEVVGFAPYINGEG
                     MLAANGQVRGALVQGILPELEPKVSILPQHMVAGTLEALKPGEYGIVMGDLLAAILRV
                     GVGDKVTLVMPEANVSPAGIYPRLKRFTVVGLFRLRAELDASLAVIHLEDAGTLFRIP
                     GEVHGLRLKLQDLFMAPRVSWEVARDLPGTFRASDWTHSHGNLYQATQLEKRMLGLLL
                     MMIVAVAVFNIVSSLVMLVVDKQGDIAILRTLGATPRTILGIFMVQGSVIGVVGTLMG
                     TLFGTLLAWTITDLVEWLERLSGHRILSSDVYFIDYFPSQLLWSDVAIVCGATLTMSF
                     LATIYPALRAARIEPAEVLRYE"
     gene            21742..22380
                     /locus_tag="PKI23_02295"
     CDS             21742..22380
                     /locus_tag="PKI23_02295"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNN35801.1"
                     /translation="MNQHKERREYYRIQDTLALEFQSVPKEQALAQTPVMPFTLDPRF
                     QVLHALHELDHESHATLRQISDADRHLGSYLRTLNRKIELIAMGMFASSNSEAPSPTH
                     DVTLSESGLSFRHEQPLAPDSHLALKLVLFPAYFGLLLFGRVVQCTPLALPDAGNQSS
                     AAPTARLFNIGVEFTHVQESERELLARYILRKQAQQRREQRNRSKPDSPENG"
     gene            22423..23460
                     /locus_tag="PKI23_02300"
     CDS             22423..23460
                     /locus_tag="PKI23_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNN35802.1"
                     /translation="MNHPRHLPAEWAPQWAVMLTWPHAGMDCQRNFAEVEALFLTLAS
                     AIAQRQQLIVSVTQPQQLDALRDHLRQAGIAAERLIGQVVPANDFWARDHGPITVLSG
                     DQPRLLDFRFNGWGGKYPAELDNQISSQLHRRGAFGSAALEPIDWVFEGGNLECDGAG
                     TLLLNRQSVLTASRNPGLNEAMVEAEFRRLLGIDRVLWISHGHIEGDDTDGHIDVLAR
                     FTDPDTLCHISTEDRDDPNFAELDAMAAELRALRKRDGTPYRLVPLPLPAAQRSLGGE
                     PLALSYANFLIINGAVLLPVYRDPADRIALAQLARCFPDREIVPIMALPLVEHGGSIH
                     CVTMQLPAARW"
     gene            23757..26114
                     /locus_tag="PKI23_02305"
     CDS             23757..26114
                     /locus_tag="PKI23_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNN35803.1"
                     /translation="MRRSTAPLRLRPLFLTLPVALTATHGNAEPSYELPTIAVEESAL
                     RAAFDGTTIDSDQLRWRRAISSDSATLLKDVPGVSLYGAGGVSSLPVIHGLADDRLRI
                     KLDGMDLIAACPNHMNPVLSYIDPTQVGSIRVYAGVAPVSVGGDSIGGAIVAETAEPK
                     FAAPGEGSLTAADVGTFYRSNGNARGANLAATYATEAVSLSYSGATAEADNYDAGGNY
                     KNFVISGIAPYVEPDEVGSTAYETRNHTLGVAIRHANHLAEAKFGYQDLPHQLYPNQR
                     MDMLDNEQKRLNLRYRGQFDWGAFDARAYHEEVDHFMDFGQDKVYNYGALMPPNTTGA
                     TYLVNGMPMYTEGKTDGVSLRGDIDLGAPDVLRIGLDLQQYRLDDWWPPAPNCGVGNC
                     VGGMAPLTFWNINDGERDRLGLFAEWEARWTPQWLSLVGIRSERVETDTGPVVGYNTS
                     TTPLSMGFMKNMYEASSVGTRADFNAMDRSRTDHNLDLSAIARYTPDANLTFEFGLAQ
                     QTRSPNLYERYAWSRNVMALEMNNFVGDGNGYLGNPNLDPEVAHTLSITGEWHSSDGE
                     YQARVTPYYTRVKDYIDAVQWNASTNLPANPVVPGQFVVMKYENQQARLYGVDVSGKM
                     PLGKTPIGTFGLTGLVNYTDGENTDTHDDLYNIMPLNARVSLTHQYGGWDNALEWVGV
                     KGKKDVSDARNEIKTPGYGLVNLRSGHAWQKLRVDVGVENLFDRMYYLPLGGAYVGEG
                     ATMSFNKEAGNVARSGMGLSGTQSMWGTAVPGMGRSIYAGISYSF"
     gene            26206..28359
                     /locus_tag="PKI23_02310"
     CDS             26206..28359
                     /locus_tag="PKI23_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNN35804.1"
                     /translation="MKNPRTTTALPRLSTMPLAMLAGLLTATPATAATPAAAEFEAIT
                     VTDERSREGAQIALDAPTSAGSRLDLTVRETPASVQIIDRDTLRSRGLTTTQQALDSA
                     VGVTAGQCFGLTCYSTRGFSGTLSLPFLYNGLRYPGLAMSPRGTFNYERIEVIRGPAS
                     VLNGFGSIGGAVNFVTKAADGVEEQELFTSFDSWSQKTVGIGVGGSATEQLAYRMDLS
                     HSGGPHGSSGFVDRTRYDYYHASGELRLAATDSFTATLAAESIKDQGEWYFGTPHRAG
                     RIIDGSEQNNYNIDDDRVAKEMHWLRLKLEQRFDDSTALSNETYFNTENRYWHNAEVY
                     ELDPALPLVRRSDFLHIAHDQELLGNLLQLKLTGPLFGLPNRLVLGYDLSRNLHQRDS
                     NSPFSSAANSVDLFDPVAGQFTTNSPFLPFRKTRLVQQALFIEDYLNLTDALKLSLAA
                     RRDWIDLTSDNLRTATGFDQQWSGDSYRAGLVHDLRPGVTLYGQWSKALEAPAQIVTL
                     SEAQRNFDLTEGRQWEVGAKADLPRKLGEVTLAWFDITRSNILTRDPLNSTLTVQIGE
                     QSSDGIELAVALRPHPQWAIDASLSLLDARFDEFNERVAGVGVSRAGNLPPDVAEKVG
                     DLWLSFYPDRDWRFAANAQYVGPRAANNANSITMQSYTTLGASVSHALAGGELTLRLR
                     NLTDALYANRSYGNSGSQFLLGEPRAVELSWHGNF"
     gene            28341..29582
                     /locus_tag="PKI23_02315"
     CDS             28341..29582
                     /locus_tag="PKI23_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35805.1"
                     /translation="MARQLLSRRGGAIAATALLLAVATLLWWLRYPAASVAASVQPIS
                     APAGAGARLPRLAAHPAGGAVLSWVEPAPEGGHLLRVARYRNGRFEAPVEVARGRDWF
                     VNWGDFPSVVPIAADFWLAHWLVRHPRADSPYHYGIAMAGSSDDGRRWTTRPAPHADV
                     GPAEHGFAAIAALDDRAAMVWLDGRANDARHSFALRTTEIERDGAAAAESIIDSDVCS
                     CCWPALARTRNQLWVAYRGRTSDEIRDFQLRYRTVAGWSAPIALAGEGWRIAGCPTNG
                     VSLAAQDERLAAAWYTAADGVARVRVAFIRDGGNALESIHDVDVATPVGRAAIAWLDS
                     ERVAALHIAASSSALADLRLTLLSDRGIERSLTLIQVPANRDSGVPQLLAVDGELLVA
                     WTDAAPGHGVRVLRVPQRALR"
     gene            complement(29598..30035)
                     /locus_tag="PKI23_02320"
     CDS             complement(29598..30035)
                     /locus_tag="PKI23_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNN35806.1"
                     /translation="MALGDGVASTARSSAEINIVPLVDVILVLLVIFIVTAPLITQAV
                     KVKLPEAKSQPVQTPSKPVVVAIAADGQLHWDGQPLSLAELEERARALAARPAAELHV
                     EADRAVRYDAVAQVLAAATRAGVLHIGFVTDPGQARSQAAPVP"
     gene            complement(30039..30593)
                     /locus_tag="PKI23_02325"
     CDS             complement(30039..30593)
                     /locus_tag="PKI23_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNN35807.1"
                     /translation="MRTILETAGLAQSPPEPFGRPFEASLLAVVRDGRRLADAGEQFD
                     EAGGLAAHLTRGLRQQLDRAAVRGERGLALLSTTASTAPYLGLLGTVWGIYHALLRIA
                     ATGEAGLAAVAGPIGEALVMTALGLAVAIPAVFAHNTLQRRHRLLQAQREDFATTLLQ
                     RATLPGSPSGAIKRERAAIHPLRS"
     gene            complement(30767..31027)
                     /locus_tag="PKI23_02330"
     CDS             complement(30767..31027)
                     /locus_tag="PKI23_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNN35808.1"
                     /translation="MHCPRRPPPQYPMAARRLGEEGVVELLVALSADGSVRQVSVSRP
                     SGFGRLDQAAVAAVRLWQCDPIRVAGVPAAATARQRIHFSLR"
     gene            31140..31445
                     /locus_tag="PKI23_02335"
     CDS             31140..31445
                     /locus_tag="PKI23_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35809.1"
                     /translation="MTGAGAGSSRSATGASSRGFLSGLGGFVLAIFAGAGVGSGIAAD
                     GGLGATGGAGGGVGRSIIRIATGWIDNAGGAGRSHNSRTTPPWSATAISHANPCSAG"
     gene            complement(31701..32954)
                     /locus_tag="PKI23_02340"
     CDS             complement(31701..32954)
                     /locus_tag="PKI23_02340"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNN35810.1"
                     /translation="MLKKITTEQVCLGMYIHGLEGSWLSHPFWKSKFVLDDPTDLRAL
                     KKSGVSAVWIDCDKGRDVAGLTDASDVAAEPAAEPTPEPAATETVAVAGKQPVAPAIQ
                     LNKTTSAQEELGRASGIIHHSKEAVARLFTEARLGKVIEVEQCLPLVEEISGSVARNS
                     TALISLARLKSKDEYTYMHSVSVCALMIALARQMQLDEQQTQQAGVAGLLHDIGKMAM
                     PEKILNKPGALTTDEFSVIRLHPERGHAILTRSGFTAPSALDVCLHHHEKIDGSGYPY
                     GLKGEQISQLARMGAVCDVYDAITSNRPYKNGWDAADSLARMAKWEGHFDTAIFHAFV
                     KMVGIYPLGSLVRLHSGRLGVIVEQNPQSLTRPLLKVFFSTRTNMPIPVQLLDLSKSG
                     QTDTIVNREDPQKWQFSNLNALWQG"
     gene            33251..35287
                     /gene="metG"
                     /locus_tag="PKI23_02345"
     CDS             33251..35287
                     /gene="metG"
                     /locus_tag="PKI23_02345"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNN35811.1"
                     /translation="MTASSRQILVTSALPYANGPIHLGHMLEYIQTDIWVRFQRLQQQ
                     RCFYVCADDAHGTAIMLKAEELGITPEALIEQVSAEHQRDFADFGISFDNYHSTHSEE
                     NRHFAELIYQRCRQAGHIETRTISQFYDPDKELFLADRFIKGSCPRCGAADQYGDNCE
                     ACGATYSPDQLIEPRSAISGATPVMRSSEHQFFRLADFTELLKQWTRSGALSEEVANK
                     LDEWLEGGLESWDISRDAPYFGFRIPGSDDKYFYVWLDAPIGYMASFKNLCDRTPGLD
                     FDHYWRPGSSSELYHFIGKDIITFHSLFWPAMLHAARFRTPTAVHVHGFVTINGQKMS
                     KSRGTFIKARTFLDHLEPEYLRYYFAAKLSARIDDLDLNFDDFVQRINADLVGKLVNI
                     ASRSAGFIHKQHAGRLAAQLPEPALWDEFVAAGEQIAEHYEAREFARVVREVMALADR
                     ANQYIDQQKPWLLAKDPARQPQVQQIASFGLNLFRLLVLYLQPILPRLAERVEGFLAT
                     PLPNWSRRNAPLLDHLIAPFTPLFTRIEPDTITALLAASQQNATETAPVISAPEPPGG
                     EAIAATIDYDSFAKVDLRIARIVAAEPVEGADKLLRLTLDLGGEQRTVFAGIKSAYTA
                     ERLIGRLTVMVANLAPRKMRFGLSEGMVLAAGPGGEQIWLLSPDSGALPGMRVR"
     gene            35298..>35634
                     /locus_tag="PKI23_02350"
     CDS             35298..>35634
                     /locus_tag="PKI23_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNN35812.1"
                     /translation="MSQPGVDAVPALVEAIDALLPQTQCGRCGHPGCRPYAEAIASGE
                     PFNRCPPGGQLTIDLLATLLERPKLTLDERHGTEQPPQVALIREEECIGCTKCIQACP
                     VDAIVGAAQW"
BASE COUNT         6314 a        12307 c        11048 g         5965 t
ORIGIN      
        1 ttcaagttca ccacgtccaa caagcagttc aactttttgc agcacatcat gaccatcctc
       61 gacacccacg gccgcgccgg cgtggtgctg ccggacaacg tgctgttcga ggccggccgc
      121 gcgggcgagg acatccgccg ccggctgctg gagggcttca acttccacac cctgctgcgc
      181 ctgccgaccg gcatctggta cagccccggc gtgaaggcca acgtgctgtt cttcgacaag
      241 cgcccggccg cgcgcgaggc gcagacgcgc gagctgtggg tctacgacta ccgcaccaac
      301 gtgcacaaga cgctgaagac caagcgcctg gagcggggcg atttcgacga cttcatccgc
      361 tgcttccacg cgcgccagga gaccgagcgc ttccgccgct tcagctacgc ggaactcgcc
      421 cagcgcgaca agctgaatct cgacatcttc tggctgaagg acgacagcct ggaggacttg
      481 ggcagcctgc ccgagccgga cgtgctggcg gcggagatcg tcgagaacct ggaggcggcg
      541 ctcacgcagt tccgcagcgt gagcctggag ctggcggggg aggagggcta aaacgcctct
      601 gcgcgccgga ttggagtgca ctccggcgct gcttcattca gcaaggttct tccgcagcca
      661 gccatcgacc agcgccgtgg cggggtaatc gggatcgccg agccggcggt tgatctccat
      721 gtggccacgc aagccagtgc cggcaaaact gccgtagtcg accgcgctgc ccgcggcggc
      781 gagcgcgcgg cccagtgcct cggtttgccg cacggcgtcg ggacgctgca cgtgcagcag
      841 caaaaaggcc ggcgcattgg gcgcggcggc ctggtgagtg ggcgaaagcg cccactgacg
      901 cgccggatcg ctgccgaaca cttcgagata ggtctgatgc atcatcggcg catcttcctg
      961 caactgcgcc ggcacgtcgt aagctgcacc gtcgttggcg atcacaccgc ggaggtcggc
     1021 aaaaccgagc ccggccgact tcagatggcg ctcgtccgtg ccgaccaggg caaccagatg
     1081 cgcgccggcg ctgtggccga tcagcaccac ccggcggcga tcgatgccca gttccgccgc
     1141 acggtcgagc agcgcccgca gtgcgtgggc gacatcctcg gcctgctgct cgaccgcgac
     1201 ggccggcacc agccggtagt cgatcgaggc ataggcgtaa ccctgttgcg tcaggtgcgc
     1261 cggcagccat ctgctcgagg cgttgtcctt gctgccgcgc tgccagccgc caccatgcac
     1321 gaacatcacc aagggcgcag catcgctgcc cggcactgtc cagacatcca accgttgcag
     1381 cgcatccctg ccataggcga tggtcctggc ggcggatttg ccgtgccggt cggggggtgt
     1441 ctgagggtca tccagcccgt cgcggggacg ggcatcggcg gcaaccgcca gcagcaggat
     1501 gacgatcgaa aagactgttc tcttgcccat cttcatgctc acttttgcgt cagcgcgcag
     1561 gcgccggtcg gaccttcaac gctgcacccg gttggcgatc acccgctggg tggccatttc
     1621 gtcaagtcgg tgctgctctt ttttgttcgc ccgcagccgg gcctggcggt cggcctcctc
     1681 ggccagttgt tgcagcccca ggcggcgcag tctcagttcg cgccagccgg cctcgagcgc
     1741 cgcatggtcg ttgcggacga tcagcagtcg gcgctcctgc tggccgatgg cgaccttgag
     1801 ccgatcgacg aaaccctggc gattctgcag catgaaggcc gctccgggca gcgggtagtc
     1861 gccgggcgtg gatgacaggt agtcgtcgag gtagccgcgc aactggtcga accgctgctg
     1921 ctcctcgcgc aactgaccgg cagcctgatt gagtgccagc agcgccgtct gttcgcgctc
     1981 ttcggccagc ttcaccactg gccagatcgc cttgctgttg atcatcggcc ggcggtttca
     2041 gcggtgttga acagctgttc cagttcacca ctgctgctgg ccagcgagaa gctttcgttc
     2101 aatccctgac gcaggtagcc gcgcagttgc ggaatcagct ggatggccag atcggtgtcg
     2161 gcatcggcgc cgcgctggta ggcgccgatc tggatcagtt cacggctgcg ctgataggcc
     2221 gagtagagct gcttgaagcg ttgcgcctgg gccagttgcc cggccgacac cacctgcggc
     2281 tgcacccggc tgatcgaggc ttcgatgtcg atggcgggat agtggccctc ttcggccagc
     2341 cggcgggcca gcagcacgtg gccatcgaga atcgcccgcg ccgagtcggc caccggatcc
     2401 tggtcatcat cgccctccat cagcacggtg tagaaggcgg tgatggagcc accgccctga
     2461 tcaccattgc cggcacgttc gaccagctcg ggcagttggg caaagaccga gggcggataa
     2521 ccacgggtgg ccggcggttc accgatggcc agcgccactt cgcgctgcgc ctgggcgaag
     2581 cgggtcagcg agtcgagcag cagcagcacg tcgtggccct ggtcacgaaa gtgttcggcg
     2641 atgcgggtgg ccagccgggc ggcccgcagc cgcatcaccg gtgattcgtc ggccggggcg
     2701 gccaccacca ccgcacgtgc cagcccttcg gcgccgaggt tgtgttcgat gaactcccgc
     2761 acttcacggc cgcgctcgcc caccagcccc acgacgacga tctgcgcggt ggtgaagcgg
     2821 gtcatcatgc cgagcaggac gctcttgccg acgccgctgc cggcaaacag ccccatgcgc
     2881 tgaccgcggc cgacggtcag caggccgttg atggcgcgga tgccgacatc gagcggctcg
     2941 cgcaccggca atcgccgcag cgggttgatc atcggtccgg caaaggggat ggtgtggctg
     3001 cattgcggcg gtggcaggcc gtcgagcggg aggcccagtc cgtcgatcac ccgcccgagc
     3061 agcgcgtcgc ccacctcgat gcgtgcctca tcgccaccgg gcagtacgcg ggcgcccggt
     3121 ttgagtccac cgatgcggcg catcggcatc agaaacagtt tgccctcgtc gaaaccgacc
     3181 acctcggctt cgatatgctg tccgttggca ctttcgatca gacagcactc ccccagcgca
     3241 gcaccgaggc cgaccacttc gagcgtcagc cccaccgagc gcaccagccg acccgacacc
     3301 agcgggcgcg gtggcgaagc ggtgtcgagc ggatagcgcg cgatccgctc cagcagctgc
     3361 ggcctggtca tgtcgaatcc accgccggat cgatgaagcg ctgcgccgcg ctcagctcgc
     3421 ggcccagcag gtttctgacc agttcacgaa agcggtactc agtggtgaaa tcgatgcagc
     3481 tgtcgcggct ttcgatccgg cagccgccac ggctgacgcc gggatcggcc tcgatggtca
     3541 gcggcagttt ctgcggcagc ttcagcagca gcgcctgctc gtcgggatgc agcagcaggc
     3601 tgacctgctc cgcgcccttc ggcagtgcgg caatggcctg gcgcaccacg ctcagtaccg
     3661 tctcgcccga gctcttcagt tcatgttcga gcaaggcgcg gctgatctcg atgaccagat
     3721 tcaacagcgc ggtctcgatc tcggcttcgc gctcggccag cggatcggcc agatgggtca
     3781 gcagccgggt caggcgtgct gcctcgttct gccagatttc gcgtccggcc cgctcgccct
     3841 cctcgcgccc cagcgcgtaa ccggccgcat ggccctcggt gtgggcggcc gccttcagcg
     3901 ccgcgatgcg cagcgccacc tgctcttcga cttcggcctg ccgcgcagca tcgggcgagc
     3961 gaatcacctc gccacttgaa ctgatctgcg gcggcgccca gcgatagagc ttgcgtacgc
     4021 tctcgacatc cagcagcaga ctcttcgtca tcagagcatc tcctcaccgc cgccggcact
     4081 gagaatgatc gtcccggcat cggccagacg ccgcgccacg gcaagaatct ctttctgcgc
     4141 ggcctcgacc tcgctgaccc gcaccggccc cttggcttcg aggtcatcgc gcagcatgtc
     4201 accggcgcgc ttcgacatgt tgccgaagat cttctgcttc agcgcctcgt cggcgccctt
     4261 cagcgccaga atcaggttct cggaggagat ctcgcgcagc agcgcctgaa tgccgctgtc
     4321 ggcaatgccg atcaggttgt cgaagacgaa catccgatcc tggatgttgt tgctcatctc
     4381 ttcgttctct tcgcgaatgc cctccatcac ctgcgcggca accgagctgt caaggttgtt
     4441 gatgatctcg gccgcagtct tgacgccacc cagcgttgcg ctctgggtac cggccccgcc
     4501 ggagaactgg cgctcgaaga tccggttcag ctcctgcagc gcctcgggct gcacggtgtc
     4561 gagccgggcg acgcgcatca tgatgtcgag ccgcacctgt ggcggaaagt tgaccagcac
     4621 ctcggcggca tggtcaccgt cgaggtaggc aatgacgatg gcctgaatct gcggatgttc
     4681 gtagcggatc atcccggcca ccgaacgggc atccatccac ttgagggtgt cgaggccgct
     4741 ggtgttgccg ccgatcagga tgcggtcggc cagactcttg gccttgtcct cacccagcgc
     4801 cgagaccagc atcttgcgga tgtactggtc gttgcccacc ccgaggccag tcaggtcacc
     4861 ggcctcatcg acaaaaccat agaccgcctg ctcgacatcg ctgcgcgaga tgttgttcag
     4921 ctcggccatt gccgcaccga cccgctgcac ctccttcggc cccatgtttt tcagcacggc
     4981 agcggcgttc tcctcaccga tggtcatcag caggatggcg gcacgctgga tcggccgcag
     5041 cgaagagttg ccgcgctcac tcaccgtcgt tcacccactt cttgaccacc tgcgccaccc
     5101 gccccggatc ctgctgcacc agtcccttga ccgcatccag ttgctgctca tagctcttgc
     5161 tgccatcgaa cgagatcatc cggcccggtt cggtgatcgg cgccgccgtc tgcatcatct
     5221 cgccgccctc tcccatcacc accgtgccgg ccgggtaggc caccagcgtg gcagggcgcg
     5281 tgcccgaaaa ggcagagatg ttcttcatca ccggccgcag gatggcaatg gcgatcagca
     5341 cgacaaagag cccacccagc agctgtttgg ccagactcca gaaccagggc tcgctccaga
     5401 aagccaccgg ctcaccagcc ggttcgattt cccgtttcac aaaggaggag ttgaccacgc
     5461 tcagggtgtc gccgcgcgcg gcgtcatagc cggtcgcacc cttgaccagt tcggtgagct
     5521 tgcgcagctc ctcgtcgctc caggcacgtc gcggcacggt gttggccgcc tccttgccct
     5581 ccgccgcacc ctttttggca ctgcctttct taggaggggc gaggtcgggc agatcgtcga
     5641 ccaccactgc gatcgacagc cggcgcacct tgcccacctg ctgccgggtg tggctcagcg
     5701 tgcgatcgag ctcgtagttg cgggtggcct gattgcggct gcggccggtt tcggcactgc
     5761 tggaagccgc tgcacccgct gcggcctcgg ccggcagcgg cggagtcgtc ggcctggcgg
     5821 ccacttccgg aacgctgacg gcaccgggcg gctggttgga gagcgccccc ggcaccccgc
     5881 cccgatctcc ggcacccccc atcatccgct cgaccagcgt ctgctcgctg cgcagcgccg
     5941 gcgggtcggg ctggtactgc tcgctggtct gctcctgttc ggtgaagtcg acatcggccg
     6001 agatctcggc ggtgaagtgg tcatagccca ccagcggctc aaccacgcgc cgcacgcgcc
     6061 caatcagcga ctcctcgatg cggcggacat gttcgagctg ctttccggca tcggccagcg
     6121 caccctcctc ctcgttgcgc gacagcagtc gtcccttctg gtcgaccacc gagacatcct
     6181 tggcatccag atcggccacg ctggaagtga ccagattgac catcgcgctc acctgatccg
     6241 gcgtcagcgc attgcccgac tgcaactcga cgaagatcga ggcgcgcggc ttgttgccgc
     6301 cacgcacgaa ggtggtcgac ttgggcagcg ccagatgcac ccgggcactg cgcaccgcgt
     6361 acatgccggt gatggtgcgg gccagttctc cttcgaggct gcgctgcagg cgggtcgcct
     6421 ccatgaactg gctgctgccg aacggtgact cctgatcgag cagttcgtag ccctgcgcgc
     6481 gttcgccacc caggccggcg ccggccatct tcatcttgac gatctgcacc ttgtcggcag
     6541 gcaccagcag cgccccgctc ctggggtcga tgtgataggg aatcttctgc gcatcgagca
     6601 ggctcaccac ccgcgcggca tcggcgccac ccagcgcggc gtcgagcggc atgtagggct
     6661 cctcgcgcga ccagagcgca acggccacac cgatggcgac actggcggcc agcccgacca
     6721 gcaggccgaa ttggcgcagc agcggcagat tgaccatgcc ggaaagcagg cccatgccac
     6781 cgctgttctg agcttgcgaa gctgccggaa gcgtctccat gttctttcaa ccctgcctgc
     6841 gttgcgaatg tactgtattg atatgaaaaa tcattctttg atcagatcgg catgttcatg
     6901 atggtctgat aggcatcgac caccttgttg cgcacctggg tcaacgcctg gaatgagagg
     6961 ctcgacttct gcatcgccac catcacctgg gtgatgtcga ccccgggctg ccccagctca
     7021 taggcctgcg ccaactgcgc cgatttgctc tgcatgtcgt tgaccccgtc gaccgcctgc
     7081 cgcaacagat cgccgaatcc gctgctggag gtgaccgctg gagcaccgat cccggccatc
     7141 tccatgccgg gcacgctgcc cacatcggaa cgctggctgt tcatctcggc cttgatcgag
     7201 cgcatctgct gcagcagctg ctgaatgtcg ctgcggtcgt tgatgcggtt caccatggtt
     7261 ctctcctgtc atcgacatgg acggactttc gacgtttttt gttcttcaaa catgttccgg
     7321 ggcagttcgg cgtcggttcc ttgacgaaac cgccctgctg acccggttct gcagcggttc
     7381 gatcagtaaa tagcagggat caggccaact ctgcggggtc atggccgcca cgcgcgggcc
     7441 agcgacagga attcgggaag ggctgaaacg acagcggcca ctgctggagt gaccgcgtcg
     7501 agaacgggat gtcgtgatgc agaccggcgt tcgctgtgcc agctcagacc tccagcgcca
     7561 aaccggcctc gcgcatctgt gccagcttgt aacgcagggt ccgctcgctg atgccgagct
     7621 gctgggcgct gtcacggcga ctgccctggc agcggcgcag tgtctccatg atcaggcgat
     7681 gctcatgcga cttcacatcg ctgccaaggt cggtggcggc ggtgactggc tcgctgtgcg
     7741 agatggcgac tctgacagaa tgggaagcgg ttccggtcag ggggccgagg tggagctgcg
     7801 agagatcgat ctcacacccc tcctgcagaa tcagcgcccg ctgcaacacg ttgtcgagtt
     7861 cgcgcacatt gcccggccac ggatgggcca gcagcgcgcg ctcggcagcc ggcgtcagca
     7921 gcggcgccgg gcgttgggca cgctgggcat ggcgggccag cagccgccgc gccagcggca
     7981 ggatgtcttc gtgccgctcg cgcagcggca gccactccag cggaaagacg ctgaggcgat
     8041 agtagagatc ctcgcgaaag cgcccctgct cgacctcctg atgcagcaga cggttggtgg
     8101 tggccagaat gcgcagatcg agttgcaggg tgcgccggcc accgatccgc tccacctcgc
     8161 gctcctgcag cacgcgcaga atcttggcct gcagcggcag cgccatctcg gagatctcat
     8221 cgagcagcag ggtgccaccg ttggcctgct cgaatttgcc cggctggctc tggtaggcgc
     8281 cggtgaaggc gcccttctca tggccgaaca gcgtcgcttc gagcatgttc tcgggaatgg
     8341 cggcgcagtt gatcgcgacg aagggctggc tggcacgggg cgagtggcgg tggatgaaac
     8401 gggccagctc ctccttgccg gtgccgctct cgccacagat cagcacattc gaatcgctct
     8461 gtgccacccg ctgcgccagc gccagcaggc gtcgactgct ggcggcctcg gccagcggat
     8521 catgctcgtc aaccggtact gctgccagat gacgccgcac cgtttcgatc agccgctcgc
     8581 tgctgaaggg tttgaccaga tagtcggccg catcctgccg catcgcctgc accgcctttt
     8641 cgatcgagcc gtaggcggtc atcagcacca ccggcaacat cggtcggctg cccttgacct
     8701 gttgcaacag ctgatggcca tccatgccgg gcatgttgac gtcacacagc agcaggccgg
     8761 gctcgatctg ttcgagccgc gccagcgccg cctcgcccga attgacgctg caggcatcga
     8821 aaccagccag ctccagggtt tcggtcaagg cctcggccag atcgaggtca tcctcgatca
     8881 ccagaatggt tgtccggttc atcatggttt ctgctcccgg attgcgggtt ggagggtgcg
     8941 gtgcatggct caggtcagcc actgcacgac cggcagccgc agctcggcac gggcaccgcc
     9001 cgggtcacgc ccttgcaggc ccggcaggtc ggtgagcaga aagtcgccat ggtgggcgcg
     9061 cgccaccgcc agcaccaccg gcagaccgag gccggtgccg ctgctcttgg tggtgtagaa
     9121 ctcaccgagc gccgcctgct cgcggccatg cagtccaggg ccgttgtcat cgacgcggat
     9181 ggcgatgcgc tcggcatcga gccgcgtcac agccacccgc aaccggggct gcgcagtggc
     9241 cgcctgcaac gcgttgttga tcagattgac caccgcaccg accagcaccg cctggttgca
     9301 gcgcagcagc gccggcggtg cctccagcag gtagtcgatg cgggcaccgg cggccgtgac
     9361 cgccacctcg gcgctgcgct cgatctgctc cagcagctgc gccagggtga tctgctcggt
     9421 cagggtcacc tcacccttga tgaacaccgt catctgccga atctgctcct ccatgtgctg
     9481 cagccgctcg gtcagccgct gcactgcacg gtcgcgccgc tcggccgaca gccgttcgct
     9541 gcgcaactgg tcgctgtaga gcatcgcggc cgccagcggc gttcgcagtt gatgggccag
     9601 cgccgccatc atcttgccga gactgaccag ccgctcatcg cggtgcagac ggcgctgcag
     9661 tgcgcgggtt tcgctctgat cggtcagcag gatcagctgt ccatgccccc catccagcga
     9721 gcgggtggcc agtgcgatca gccggccatc cttcagcgag acttcatggc catcaccggc
     9781 ctgtggtgcg aagcagcgct cgatcaccgt cgaccagcgc gctccgagca gcggttcgcc
     9841 cagcagctcg atcgcggccg gattgcactc ggtgacgatg ccgcgttcat cgagcaccac
     9901 cacaccaccc ggcaacagcc gcagcagcgc ctcgtgccgg tcggccagcc gttccttctg
     9961 tgccagttca tgccgtcgct cggcactctc gctggccagc tcatcacgca gttggttgac
    10021 ccgttcgctc agtgcccgat aggcaccgga cgcagcgggc gggtgtgcgc tcaggccgac
    10081 actttcaggc cagtgaagcg ttgtctgagg cgcgaaggga acagggtgca gcatgggcat
    10141 cggtcaccat tccgtgagtc agggagggca gcgcggcgga aattcgtcgc gctgcggcac
    10201 tgttccctga aaatgcaacc gccatgccta ttttattttt ctttttattc aacaagataa
    10261 aaacacaagc cggattgttg acggctactc ccactccttg cgctgcatgc cgtacttgcg
    10321 catcttctcg accagcgtgg tgcggcgcag gtgcaactgc tcggcggcgc gcgccaccac
    10381 accgttgttg tagtcgagcg actgctggat cagcgtcgtc tccagatggc tgatgtactc
    10441 gcgcaggtcg acaccgttga gcggcagctt gagatcgcgc ggcagcgctt cgtcctgctg
    10501 ctgtggcggt gtggagagcg gctgcgccat ggtgggcagc gcctgaggaa ttggccgagg
    10561 ttcaccctcg ggcaggaagg tctcgacctc gctgtcgtcg tagcggtact tcttcggcag
    10621 gtcatgcacg ccgatcacgc catagggatg catgatcgcc atccgctcga tcagattggc
    10681 cagctcgcgc acattgcccg gccagtcatg gcgacagagc gacatgatcg ccgccgagct
    10741 gaagcggggc gcgccacgct cttcgtgctc gatgcgggcg atcagctcct gcagcagcag
    10801 gctgatgtcc tcacgccgct cgcgcagcgc cggcatctcg atcggaaaga cattgagccg
    10861 gtaatagaga tcttcgcgga actcgccgcg ctcgatcatc gcttcgagat ccttgtgggt
    10921 ggcggcgatg acccgcacat cgatctcgat cggccggctg ccaccgaccc gctcgatggt
    10981 gcgctcctgc aacacccgca gaatcttgac ctgcatgttg agcggcatgt cgccgatctc
    11041 gtcgagcagg atggtgccgc catgggccat ctcgaagcgg ccggcccgcg cggtgatggc
    11101 gccggtgaag gcgccctttt cgtggccgaa cagctcgctt tcgagcagct ccggcggaat
    11161 ggcaccgcag ttgatcggta caaagggctt gtcgcggcgc ggcgagtggt agtggagatt
    11221 gcgcgccacc acctccttgc cggtgcccga ttcaccggtg atcagcacgg tggcatcgcg
    11281 gtcggcgaca tggttcatca tctcccgcac cttctggatc gcacggctgg tgccgaccag
    11341 actgcggaac aggtggatct cacgccggcg cggcgcgctc tcaccacgcg cccgctcatg
    11401 ctgatcacgg tagacccgtg ctcgatgcag gtggtcgatc agccggttgt aaccgagcgg
    11461 ccaggcttca cgggcgatca ccatgcgttt cagttcgttc ggcacgctct catccgcggc
    11521 ttggccactg atgtcgatga cggcgacaca gccatcccac tggtgaagct gggtcagcaa
    11581 ctccggcagg gaacctccct cggcgcggct gcccagcagc actgccactg gcgggccctc
    11641 ggcctgcggc gcggctttca gttgctcccg ccagccatga ctggccacca ccacagccgc
    11701 ttcacccaga aaatcggtga tgatcctcag ttcggcctgc cgcgccggct catcttcgat
    11761 caccagcaac cactgctcat tgcgcatcga cgctcgatcc tcctgcttcc actgctccgc
    11821 atcccgtcag accggtttgg gagtcaaaca actgtcagcc ctgccttgag cctagaccat
    11881 tttctgacgc cgcgccgaga ttggccggca atttttcgtc acggcattcg catctgccag
    11941 agccggttga tccgttcgag atcttccggg gtgtcgaccc ccggtggcgg cgtgacctgc
    12001 gccagctcga cccggatgtc gatgccctga ctcagcagcc gcaactgctc cagtgcctcg
    12061 atccgctcca gcgtgccgat cggccagcgg acgaaccgat gcagcagccc ggcacggtag
    12121 gcatagagac cgatgtggcg cagcgccggc acgcccgcct gctgccaggc cacatcgctg
    12181 ccgagcgcaa tgccacgtgg atacggaatc ggcgcgcggc tgaagtagag cgcatggccg
    12241 cgctcgtcac gcaccacctt gacgacattg gggttgaaga agtcctcccg ctcgacgatc
    12301 ggctcgcaga gcgtggccat cggtgcggtg ccatgcagca gtgccgccgc cacctggtcg
    12361 agcagttgcg gcggaatcag cggctcgtcg ccctgcaggt tgaccacgat ctgctccggc
    12421 gcgtagcggc gcagccgcac cacctcttcc agccggtcgg tgccggaggc atgttcggtc
    12481 gaggtcatca ccacttcggc gccaaaaccg cgcgccacct cggcgatacg cagatcgtcg
    12541 gtggcgatca ccacctcccg tgcctgactg cgccgcgcct gctgatggac ccgctggacc
    12601 atcggcaggc cacccaccaa tgccagcggt ttgcccggca agcgggatga ggcatggcgg
    12661 gccggaatca ccaccacgaa atccactgct cactgctcct tggggggtgt ggcaccgcag
    12721 ggctgcgcca actgtgccaa ttgcacatgc aacgcctgcc agaagcgctc cggcagttcg
    12781 gcctcgaccc gcagccacca atggtgcggg cgggcgaagg cggcacattt gacggcatct
    12841 ttctcggtca tgatcaacgg atggccgtcg gccggcagat cggccgcgac aaaggggtgg
    12901 tgatcggcaa acggatgttc gatcgggtcg aaacccaact cgcgcagcgt ggcgaaaaag
    12961 cgctgcggat ggccgatgcc ggccagcgca tggacccgac gctcaccctg ccaggcggtc
    13021 acttcgatct cttcgccggt cgccagattg accaggtgct gcggcttgag ccgcatcgcc
    13081 accgcaccat cggactcact gccggcagcg ccgttgacca cccggtaatc gacactgtcg
    13141 agccggtcga gcgggctgcg cagtggtccg gccggcagca gcaggccatt gccgaagcgg
    13201 cgctgaccat cgatcacgac gatttcgatc tcgcgcgcca gccggcggtg ctgcaggcca
    13261 tcatcgcaca gcagcagatc aacctcttca gcggccagca atgctcgcgc ggcacgcacc
    13321 cggtcggggt cgatcatcac cggcagaccc gtgcgccgcg ccagcagcag cggctcatca
    13381 ccacaccggg tcgcatcctc ctctgattcg acccgatggg gcaggctgga cggtgccgcg
    13441 ccatagcccc ggctgagtat gcccggccga tagccccagg cttgcagctg ctcggccaat
    13501 gcgatcacca gcggggtctt gccggtgcca ccgaccgtga ggttgccgac caccagcacc
    13561 ggacagccca cctgccgtgc agagaggatg ccgtgccgat agagccattc acgcagcgcc
    13621 agcagccgtc cgtagagcca ggccagcacc ctgctgccgg gcagcggcgg ccctccccca
    13681 taccatgcgt gcagcagccg cgcctcgatc cgaccgcgca acgtcatggc gtgaccacgg
    13741 gcgccgcctc gtcggcgaac tgccgttcat acagtgcggt gtaggcaccg ttgcgcgcca
    13801 gcagctcggc atggctgccc gactccagca gttcacctcc ctcgatcacc agaatctggt
    13861 cggcgtggat gatggtcgag aggcgatggg cgatcaccag cgtggtacgg ttcttcatca
    13921 cctcctccag cgccgcctgg atcttctgct ccgactcggt gtcgagcgcc gaggtggcct
    13981 cgtcgagaat caggatcggc gcatccttca gaaaggcgcg ggcaatggcg atgcgctggc
    14041 gctgaccgcc ggagagcatg acgccatcgt ctccgaccag ggtgtcgaag ccgtcgggca
    14101 gcttctcgat gaactccagc gcatgggcgc gccgtgccgc ttcgcgaatc tgcacctcgc
    14161 tgcgaccggc cgtggcgcca taggcgatgt tgttgcgcac cgagtcgttg aacagcgtga
    14221 tctgctgcga gacataggcg atctgggcgc gcaggctggc cagtcgcaac tgcccgatct
    14281 cgatgccgtc gagggtgatc tggccggatt ggtggtcata gaagcgcggc agcaggctga
    14341 cgatggtcga cttgccgctg ccggagcgcc ccaccagtgc caccatcttg ccagccggaa
    14401 tcgagaagga gatctgcttc aacaccggca actcaccgcc gtggtaacca aagctgacat
    14461 cgcggtagtc gatgtcgccg cgcgcccgct cgatgctgag cgtgccgccg tccggttccg
    14521 acggctgatc gagcatgtga aagatctcat cggccgccgc cagaccgcgc tggatgatcg
    14581 actgcacatc ggccagcatc ttcgccggac gtgccagcag gctggccgca gtgatgtagg
    14641 cgacgaagcc accgggactc aactgatcga ccagcgtcgg atcgagcgcc agccacatca
    14701 gcagcgccat cgccagcgac accagcagct gcaccgccgg tccgttgctc gcttcggtcg
    14761 cggtcagctt catcagctgc tgacggttga agttgctgac ccgctggaat cgcgcctgtt
    14821 cactcgcctc gccgccgaag atgcgcacct cgcggtagcc attgatcatt tcaccggtga
    14881 catgggtgac atcacccatc gagtgctgga tgcgccggct gatgcgtttg aagcggcgcc
    14941 cggcatagcg gacgatgaag aagatcggcg gcgccaccac cagcagcagc agtgacagct
    15001 tccagttcat ccacagcagg taggccacca gtccgatcag gaacagccca tgctccagca
    15061 tgatcttgcc ggccttggtg atggcatcgg tcacctgcgt gacctgaaag gtgatcatcg
    15121 agatcagatg cccgcccgca tgggtgtcgt aatagaccgc cggcagcgtc atgaacttgc
    15181 cgaacaccag ttgccgcagg tcgaacacca gcgcgttgct gacataggcc atcgagtagt
    15241 tgccgacaaa actgcccaga ccgcgcccgc cgatcagggc caccatcgcc aacggaatga
    15301 aggcgcgata gcccggctgt ttctggctca gggagtcgat gatggtcttc atcagctcgg
    15361 cggcgccgat gctggcaccc gacatcagca gataaccgaa gagtgccagc agcagaaact
    15421 tccagtaggg gtaggcatgg ccaagcagcc ggcgatagac catccagtcg gtgcgagcca
    15481 cttcggtggc atcgggcgtt cgattcattg cgacctgtga ttgacagcgc ccggtggcgg
    15541 gcggtcgaca attatccccg attcaaccgg cagacccaaa ccgccgtcac tgtccggccg
    15601 gctgctgggt ggtgatgctg agccgggcaa aaccgagctg ggccgcggca tccatcgccg
    15661 tcaccaccgc atggtgcggg gtctgccggt cggcggtgat gacgaacggc aggttgcggt
    15721 cactgccgac ctgggcgatc agcgccgcct tcagagcatc gaggctggca ttgggcagtg
    15781 gcagggcgtt gaggcggtac tcgccgctgg cgctgatggt gatttcgatc cgcttctcgt
    15841 cgaccggcgt ctgtgcgcca ccggcctcgg gcaggtcgac cttgagattg ctctgcttgg
    15901 tgaaggtggt cgagaccatg aagaacagca gcagcacgaa catcacgtcg atcagcgagg
    15961 tgacgttgat cgcggtgtcg atcggatcct cgcggcggcg gcgaaacttc atcggcgcgc
    16021 ctgcatggcc ggcgcaaaac gggaatggag ggtcatgcag cgcacctcaa ccaagatcac
    16081 gttcactgtg cagcacctcg accagcttgc gtgcctcctg ctccagttcg accaccagat
    16141 tgtcgacccg gcgcagaaag tagcggtgca gcaccagcgc cgggatggcg acgctgagac
    16201 cggccgcggt ggtgatcagc gcctcggaga tgccgccggc cagcgacgag acattggtgg
    16261 cgccctgccc catcatcacc gcaaagatct tgatcatgcc gaagacggtg ccgagcagcc
    16321 ccagcagcgg tgagatcgct gcgacgatgc cgagcgtgtc gaggtaacgc tccagctcat
    16381 gaatgacatg gccggcctcc tcctcgatgc actccttcat gatgttgcgg ccgtatttgg
    16441 agttggccag ccccaccgcc agaatgcgcc cgagcggcga ctcctccttc agcttcttca
    16501 gcttgtcgct gtcgagctgg ttgttcttga tccagcccca cacctgaatc agcaggttgt
    16561 ccggcaggat gcgacggcga ttcagtgccc acgcccgctc gaagccgatg gccagtgcaa
    16621 tcaccgagca gagcagaatc ggcagcatca cccagccgcc ggcaacaatg atctccagca
    16681 cctgtcattt ctccttgcgc gaatccgatt catggggcat cagcgccgca tcggcagccg
    16741 acgcggacag ccgatcgtac cagtaacgcc gtgcctgttg ccgtgccaga cttggcggcg
    16801 ccatcccgcc cccactttgt atagcgtagc cgacggcgcc agtggcggcg ctattgtaga
    16861 gagtcgcgcc ggtttcggca taccgctgca ccacctgcgg atgcggatgg cggtagctgt
    16921 tgcggtaacc ggccgagaag atcacctgtt tcgcggccac tgcatcgatg aagtccggca
    16981 tcgacgacga attgctgcca tggtgcggcg ccaccagcag cgtggcctgc agcgcactcc
    17041 cccagcgttg cagcagttgc cgctcggcag ctgcctcgat gtcgccggtc agcaggatcg
    17101 actggtcacc cgatttgatc tgcaacacac aggagttgtt gttgtcactg ccgaaccgcg
    17161 gcaggtccgg atgcagaaaa cggaattcca cctcctgcca gcgccagcca tcgccactgc
    17221 ggcagggatg cagatcgatc tggtcgcgct gcaggcccca cagcgaatca ccgctgacat
    17281 agaccctgcc gaccggaacc tcccgcagca gccccgccgc gccgccatca tggtcgcggt
    17341 cggcatggct caccaccagg gcgtcgatca cggttcggcc acgccagcgc agataggggg
    17401 cgatgatctg actgccggca tcgaaccgct cactggcatg ggggccggtg tcatagatca
    17461 gcagccgctc acccacctcg accaccaccg ccaacccctg cccgacatcc agcaccgtca
    17521 gccgcaacgg ctccgagtcg gtgtgccgca ccggcatcag ccacagcggc aacaacaaca
    17581 gcaacgccca gggccgtccc ggcaaggtgc gcggcaacag ccagagcagc agcgccggca
    17641 gcaataacca cagcagccac gctggcggct gaatcacccg ggccgcggcc agcggcgagg
    17701 ccgccatcca gtgcagaaag cgatcggtca ggtcgagcag ccagtcaccg ccagcaaaga
    17761 gcagatcggc cagggtcgac gacagtggcg tcagcagcgt ggccagcagc aacagtggca
    17821 ccagcagcag gtcgaacagc ggcaccacca gcaggttggc gatcggcgaa gccaggctgg
    17881 cgctgtagtt ccaccacagc agaatcggca acatcgcaaa gaacagtccc cactgcgcca
    17941 gccaccactc ccgtccaaag cgacgcagcc ggctccactc ctgctgctgc gtgccctgaa
    18001 acagcagcat cagcgtcatc accgcaccaa aggagagcca gaaaccgctg ctgctcggcg
    18061 ccagcgggtc gctcagcagc accaggctga gcgccagtac cagcccgaac caggggtcga
    18121 tctgccgccg cagcagcttc gccgctgccc agatggcggt catgatcagg gcgcgggttg
    18181 ccgccagcgc aaaaccggac agggcggtgt agagaaaggc gccgagcagc gcgctccagg
    18241 ctgcgacgct gccggccgac cagagcagtc ccggggccag cagatggcgc ccaacccatc
    18301 cgcccagttg atgacagcac caggccacca gcgcgatgtg cagtccggag atcaccatca
    18361 gatggatggt gccggtggcg ccgaacagcc gccactcctg cggcgtcaac tgcgaacttt
    18421 caccgatgat cagcgcccgc atcagaccgg catggcgtcg ctcgtgcagc tgcggcacca
    18481 acagcccatc gacccaggcg cgcaactggc tgtcccaggc gtgccagccg gccggggcac
    18541 tgcgcaggtt gtcatcgcca ctgcgcacat aaccggtggc atcgatgccc tgccggaaca
    18601 gccaggcttc gtagtcgaac ccccccggat tgaccgaacc atgcggccgc ttgagccgca
    18661 gccgcaactg ccacttctct ccgggtgcga ccggcggcgc atcacgccaa ctgagccgca
    18721 accgtcccac ccgctccaat gcggccggca cctcaccgtc gggttcggag ggcagccagc
    18781 gctcgacatc gaacagaaac agcgcctccg gccgttgcgc agcgccggtg cgccactgcg
    18841 gcaggctgcc gaccactcct tcgacccgca gatcgacccc ctcgaattcg gcgggcagcc
    18901 tgctggcgag accgagacaa ccatagccgg tcgcccacga cagaccgatg aaaaatgcca
    18961 gtgccggcgc cactgcccta tacttgcagc gaagcagcgc cagaagcacg acgcaactgg
    19021 ccagcagagt ccggtcgggc agttctggca gcagtgcgac cagcgcaatg ccgccacaca
    19081 gtgccaacaa ccgtgcaacc attggcaccc tttttgatcc aactccgttg cgtgccccga
    19141 tcatcatgct gtcatcgaaa ccggattagc ttccattcat gcccagacgc atttttaaac
    19201 ggatcgcccc cgatccgcac aagatcaggg agcagcgcgc actgcgtttt ctcggtgaaa
    19261 cgattcacca tccagcgctg tggcacttca gccgccgctc ggtggcgacc gcctttgcca
    19321 tcggcatctt ctgcgcgatc ctgccgatgc ccggtcagat ggtgctggcc acgctgctgg
    19381 cgctctggct gcgctgcaac attccgattg ccgctggact tgcctgggcc accaacccga
    19441 tcaccgcccc gccgattctc tatgtgacct accgcaccgg ctgctggctg ctggggcagg
    19501 agccgcagat cgatcacatc gaggtttcgt tcgagtggct caaggagttc gcgctgagcg
    19561 cctgggagcc gctctgggtc ggctcggtgg tggtcggcct gctgctcggt gcggcgagct
    19621 atctggcgat ccgcatggtc tggcgctggc acatcacccg gaagtggcag cgtcggactc
    19681 cgacccgtcc cgcgctcaat cctgtggcag cagccgtccc gcttccagcc gcaatacccg
    19741 atccatccgc tcggcaaatg cctggtcatg agtgatgacg acgaaacagg tgtcgccgcc
    19801 ttcgctgagg cgggccatca gctcctgaat ctcgttcgag gtgcgtgagt cgaggttgcc
    19861 ggtcggctca tccatcagca ccaatgccgg accattgacc agggcccgcg caatcgccac
    19921 ccgctgacgt tcgccacccg agagctcgct cggcttgtgc tgcaaccggt ggcccagccc
    19981 gacctgctgc aacatctgcg tcgcctgctc gcgcgccagc gccacggcgt ggcggttgag
    20041 cagcagcggc agcgcgacat tctccagcgc cgtgaactcc ggcagcaggt ggtggaactg
    20101 gtagacgaag ccgatctgct cgttgcggaa tcggcacagc gcacgctcat cgagcgtctt
    20161 cagatcgatg ccggccagtc tgacctgacc ggcggtcgga tggtcaatgc cgcccagcag
    20221 attgagcagg gtggtcttgc cggagccgga gctgccgacc accgccaccc gctcgccgcg
    20281 ccaggccgcg aaatcgacac cgcgcagcac ctgcacctct tcgggcccct ggtggtaggt
    20341 cttgatcaga ccactgccct gcagcaccgg ctgcgctgat ctgctactca taacgcagca
    20401 cctcggccgg ttcgatgcgg gccgcccgca acgccggata gatggtggcg agaaagctca
    20461 tcgtcagcgt ggcaccgcag acgatggcga cgtcgctcca gagcagttgc gagggaaagt
    20521 agtcgatgaa gtagacatcg gagctgagaa tgcggtggcc gctcaaccgc tccagccact
    20581 cgaccaggtc ggtgatggtc caggccagca gggtgccgaa cagcgtgccc atcaaggtgc
    20641 ccaccacacc gatcaccgac ccctgcacca tgaagatgcc gagaatcgtg cgcggcgtcg
    20701 cgcccagcgt gcgcagaatg gcgatgtcac cctgcttgtc gaccaccagc atcaccagcg
    20761 aggagacgat gttgaacacc gccaccgcga cgatcatcat cagcagcagc cccagcatcc
    20821 gtttttcgag ctgggtggcc tgatagaggt tgccatggct gtgggtccag tcactggccc
    20881 gaaaggttcc gggcagatcg cgcgccacct cccagctcac ccgtggcgcc atgaacagat
    20941 cctgcagctt cagccgcagg ccatgcacct cgccggggat gcggaacagc gtaccggcat
    21001 cctccagatg aatcaccgcc agcgaggcat ccagctcggc ccgcaaccgg aacagcccga
    21061 ccacggtgaa gcgtttcagc cgtggataga tcccggccgg cgagacattc gcctccggca
    21121 tcaccagcgt gaccttgtca ccgaccccca cccgcaatat cgccgccagc aggtcgccca
    21181 tgacgatgcc atactcaccc ggcttcagcg cctccagcgt gccggcgacc atgtgctgcg
    21241 gcaggatcga caccttgggc tccagctccg gcaaaatgcc ctgcaccagg gcaccgcgca
    21301 cctggccgtt ggccgccagc atcccctcgc cattgatgta aggggcaaag ccgaccacct
    21361 caggatgctt ctgcaactgc gctcccagcg actgccagtc ggccagcgcc ccctgctcgg
    21421 gaaagacggt cgcgtgcggc accatgccca gaatgcggtc acgcagttcg cgatgaaagc
    21481 cattcatcac cgacagcacc acgatcatca ccgtcacccc cagcgtcagg ccgatcgccg
    21541 acaccagcga gatgaacgag atgaagtggt tgcggcgctt ggcacgggta taacgcagcc
    21601 cgatgaagag cggcagcggt ctgaacattc ggcatcaacc tgtgggagaa attttcggct
    21661 caccaatgcg ctgccgatcc catgctagac tttttttatc catggaatca gagcaggcaa
    21721 gaccagaagc tggatcacga catgaaccaa cacaaggagc ggcgcgaata ttaccgcatt
    21781 caggacacgc tggcgctgga attccagagc gtgcccaaag agcaggcgct ggcacagaca
    21841 cccgtcatgc cattcacgct ggacccacgc tttcaggttc tgcacgcgct gcatgaactc
    21901 gaccatgagt cacatgccac cctgcggcag atcagcgatg cagaccgcca tctgggcagc
    21961 tatctgcgca ccctcaaccg caagatcgaa ctgatcgcca tggggatgtt cgccagctcg
    22021 aacagcgagg ccccctcccc gacccacgat gtcaccctga gcgagagcgg actgtcgttc
    22081 cgccatgaac agccgctcgc gcccgacagc catctcgcgc tgaagctggt gctctttccc
    22141 gcctacttcg gtctgctgct gttcggccgg gtggtacaat gcactccgct cgccctgcct
    22201 gacgccggca accagagctc ggccgcaccc acggcgcggc tgttcaacat cggtgtcgaa
    22261 ttcacccatg tccaggagag cgaacgggag ctgctggccc gctacatcct gcgcaagcag
    22321 gcgcagcagc ggcgcgagca gcgcaatcgc agcaaaccgg attcacccga gaatggctga
    22381 gcctgcccat cggttttcac cgccgccttc gagagatcat cgatgaacca cccccgccac
    22441 ctgcccgccg aatgggcacc gcagtgggcc gtcatgctca cctggcccca tgccggaatg
    22501 gactgccagc gcaacttcgc cgaggtcgag gcgctctttc tgaccttggc cagcgcaatc
    22561 gcccaacgcc agcaactgat cgtaagcgtc acccagccgc aacagctcga cgccctgcgc
    22621 gaccatctgc ggcaggccgg cattgcggcc gagcggctga tcggtcaggt ggtgccagcc
    22681 aatgatttct gggcacgcga ccatggtccg atcaccgttc tgagcggtga ccagccgcga
    22741 ctgctcgact ttcgcttcaa cggctggggc ggcaagtacc cggccgaact cgacaaccag
    22801 atcagcagcc agctgcatcg gcgcggcgca ttcggcagcg ccgccctgga gccgatcgac
    22861 tgggtcttcg agggcggcaa tctggagtgc gatggcgcag gcaccctgct gctgaaccgg
    22921 cagagcgtgc tgacggcgag ccgcaatccg ggattgaacg aggcgatggt cgaggccgaa
    22981 ttccgccgcc tgctcggcat cgaccgcgtg ctgtggatca gccatggcca catcgagggt
    23041 gacgacaccg acggccacat cgacgtgctg gcccgcttca ccgaccccga caccctctgc
    23101 cacatcagca ccgaagatcg ggatgacccc aacttcgccg aactcgacgc catggccgcg
    23161 gagctgcgtg ccctgagaaa gcgtgacggc accccctatc ggctggtgcc gctgccactg
    23221 cccgccgctc agcggagcct cggcggtgaa ccgctggcac tcagctacgc caactttctg
    23281 atcatcaatg gcgcggtgct gctcccggtc taccgcgatc cggccgaccg gatcgccctg
    23341 gcgcaactgg cccgctgctt ccccgaccgc gagatcgtgc cgatcatggc gctgccactg
    23401 gtcgaacatg gcggcagcat ccactgcgtc accatgcaac tgccggccgc acgctggtaa
    23461 gcaccaacac gaagccggca cttcagccac agcgcactcc ccgatcgaaa ccgctctgct
    23521 gcaccagatc ggcaaagccc cgacacgcct gccatgcagg catggatcgg tgacgagaag
    23581 attgcgccgt ccctgccctg cgacaatttg tcacattttt cctgcccgct cgcctgagta
    23641 acatccctgc cgcaaccggc caggtcgatc acgattgggt cggctccctt gattcgaatg
    23701 atgcgcatcg cgaattgaac ccatccgcgc cccagcctgc cttccggtga ccgagaatga
    23761 gacgatcgac cgcccccctg cgactgcgac cactgtttct gacactgcca gtggcactga
    23821 ctgccaccca cggcaacgcc gaaccctcct acgagctgcc gacgatcgcc gtggaggagt
    23881 ccgcgctgcg cgcggccttc gatggcacca ccatcgacag cgaccaactg cgctggcggc
    23941 gcgccatctc cagcgacagc gccaccctgc tgaaggatgt gcccggtgtc agcctctacg
    24001 gcgccggcgg cgtctccagc ctgccggtga tccatgggct ggccgacgac cggctgcgca
    24061 tcaagctcga cggcatggat ctgatcgccg cctgtcccaa tcacatgaac cccgtgctct
    24121 cctacatcga cccgacccag gtcggcagca tccgcgtcta tgccggcgtc gccccggtca
    24181 gcgtgggcgg cgacagcatc ggcggcgcca tcgtcgccga aacggcggag ccgaaattcg
    24241 ccgcaccggg cgagggcagt ctcaccgccg ccgatgtcgg caccttctat cgcagcaacg
    24301 gcaacgcccg gggcgcgaac ctggccgcga cctatgccac cgaagccgtc agcctgagct
    24361 actcgggcgc caccgccgag gccgacaact acgacgccgg cggcaactac aagaacttcg
    24421 tgatatcggg catcgcaccc tacgtcgagc cggacgaggt cggctccacc gcctacgaga
    24481 cgcgcaacca tacgctgggc gtcgcgatcc ggcacgccaa ccacctggcg gaagccaagt
    24541 tcggctacca ggacctgccc caccagctct acccgaacca gcgcatggac atgctcgaca
    24601 acgagcagaa acggctcaac ctgcgctacc gcggccagtt cgactggggt gcgttcgacg
    24661 cccgcgccta ccatgaagag gtcgaccact tcatggattt cggccaggac aaggtctaca
    24721 actacggcgc cctgatgccg cccaacacca ccggcgccac ctatctggtc aacggcatgc
    24781 cgatgtatac cgagggcaag accgatggcg tttcgctgcg cggagacatc gatctcggcg
    24841 cgcccgacgt gctgcgcatc ggcctcgacc tgcagcaata ccgcctcgac gactggtggc
    24901 cgccagcccc caactgcggc gtcggcaact gtgtcggcgg catggcgccg ctgaccttct
    24961 ggaacatcaa cgacggcgag cgcgaccgcc tgggcctgtt cgccgagtgg gaagcgcgct
    25021 ggacgccgca gtggctgtcg ctcgtaggca tccgcagcga gcgggtcgag accgataccg
    25081 gcccggtggt gggctacaac accagcacca cgccgctgtc gatgggtttc atgaaaaaca
    25141 tgtacgaagc cagctcggtg ggcacgcgcg cggatttcaa cgccatggac cgcagccgca
    25201 ccgatcacaa cctcgacctg tcggccatcg cccgctacac ccccgatgcc aatctcacct
    25261 tcgagttcgg ccttgcgcag cagacccggt cgcccaacct gtacgagcgc tacgcctggt
    25321 cgcgcaacgt gatggcgctg gagatgaaca actttgtcgg cgacggcaac ggctatctcg
    25381 gcaatccgaa cctggacccg gaggtggcgc acaccctgag catcaccggc gaatggcaca
    25441 gcagcgatgg cgaataccag gcccgcgtca cgccctacta cacccgcgtc aaggattaca
    25501 tcgacgccgt gcagtggaac gcctccacca acctgccggc caatcccgtg gtgcccggac
    25561 agttcgtggt catgaaatac gaaaaccagc aggcccggct ctacggcgtc gatgtgtcgg
    25621 gcaagatgcc cctcggcaag acgcccatcg gcaccttcgg tctcaccggc ttggtcaact
    25681 acaccgacgg cgaaaatacc gatacccacg acgacctcta caacatcatg ccgctcaatg
    25741 cccgggtctc gctgacccat caatacggtg gctgggacaa cgccctcgaa tgggtgggag
    25801 tgaaagggaa aaaagacgtc tccgacgcgc gcaacgagat caagaccccg ggttacggcc
    25861 tggtcaacct gcgcagcggc catgcctggc aaaaactgcg cgtcgacgtc ggcgtcgaga
    25921 acctgttcga ccgcatgtac tacctgcccc tgggcggtgc ctacgtcggc gagggcgcga
    25981 ccatgtcgtt caacaaggag gccggcaacg tcgccagaag cggcatggga ctcagcggca
    26041 cgcagagcat gtggggcacc gcggtgcccg gcatgggtcg ctccatctat gccggcatca
    26101 gctacagctt ttgaatgacc gacatgcgca acccggcgcc tgcgcacgct gccgtgtcgc
    26161 gtgttgaccc gctgctccat tcctgcgaca gaagaagagt gaccgatgaa gaatccacgc
    26221 accacgaccg cattgccccg cctctccacc atgccgctcg cgatgctggc gggattattg
    26281 accgccacac cggccactgc ggcgacacct gctgccgccg aattcgaagc gatcaccgtc
    26341 accgatgagc gcagccgcga aggcgcgcag attgcgctcg atgcgccgac gagcgcaggc
    26401 agccggctcg acctgaccgt gcgcgagacg ccagccagcg tgcagatcat cgaccgcgac
    26461 accctgcgca gccgcggtct caccaccacc cagcaggcgc tcgacagcgc ggtgggggtg
    26521 acggccggac agtgcttcgg tctgacctgc tactccaccc gcggcttttc tggcacgctc
    26581 agcctgccgt tcctctacaa cggcctgcgc tatccggggc tcgcaatgtc gccgcgcggc
    26641 accttcaact atgaacggat cgaggtgatc cgcggtccgg cctcggtgct gaatggcttt
    26701 ggcagcatcg gcggggcggt caatttcgtc accaaggcgg ccgatggggt ggaagagcag
    26761 gagctgttca ccagtttcga cagttggagc cagaaaaccg tgggcatcgg ggtgggcggc
    26821 agcgccaccg aacagttggc ctaccggatg gacctcagcc attcaggcgg cccgcatggc
    26881 tcgtcgggct tcgtcgatcg cacccgttac gactattacc atgccagcgg cgagctgcgc
    26941 ctggcggcca ccgacagttt caccgccacg ctggcggccg agtcgatcaa ggatcagggc
    27001 gagtggtact tcggcacccc gcaccgcgcc ggccgcatca tcgatggcag cgaacagaac
    27061 aattacaaca tcgatgatga ccgggtcgcc aaggagatgc actggctgcg cctgaagctc
    27121 gaacagcgct ttgatgactc gaccgcgctc agcaatgaaa cctacttcaa cacggagaac
    27181 cgctattggc acaacgccga agtctatgaa ctggacccgg cattgccact ggtacgccgc
    27241 agcgacttcc tgcacatcgc ccatgaccag gagctgctcg gcaacctgct gcaactgaaa
    27301 ttgaccgggc cactgttcgg tctgcccaac cggctggtac tgggctacga cctgtcgcgc
    27361 aacctgcacc agcgcgacag caactcaccc ttttccagtg ctgccaacag tgtcgatctt
    27421 ttcgatccgg ttgccgggca gttcaccacc aactcgccat ttttgccatt tcgcaagacc
    27481 cggctggtgc agcaggcgct attcatcgag gattacctga acctgaccga cgcgctcaag
    27541 ctgtcgctgg cggcacggcg cgactggatc gacctcactt ccgacaacct gcgtaccgcg
    27601 accgggttcg accagcagtg gagcggtgac tcttaccgcg ccggtctggt gcatgacctg
    27661 cgcccgggcg tcaccctcta cggccagtgg tcgaaggcgt tggaagcccc cgcgcagatc
    27721 gtcacgctga gcgaagcgca acgcaacttc gatctgaccg aagggcggca gtgggaggtt
    27781 ggtgcaaaag ccgatttgcc gagaaagctg ggggaagtga cgctggcctg gttcgacatc
    27841 acgcgcagca acatcctgac ccgcgatcca ctcaactcca ctttgacggt gcagatcggt
    27901 gaacagtcct ccgacggtat cgaactcgcc gtggcgctgc gcccacatcc gcaatgggcg
    27961 atcgatgcca gcctgtcact gctcgatgcc cgcttcgatg agttcaacga gcgtgttgcg
    28021 ggtgtcggcg tgtcacgcgc cggcaacctg ccacccgacg tggccgagaa ggtcggcgac
    28081 ctctggctca gcttctaccc ggaccgcgac tggcgattcg ccgccaatgc ccagtatgtc
    28141 ggcccacgcg cggccaacaa tgccaattcg atcaccatgc agagctacac cacgctgggc
    28201 gcatcggtga gccacgcact ggcaggtggc gagctgacgc tgcggctgcg caacctcacc
    28261 gacgcgctct acgccaaccg ctcctatggc aacagcggtt cacagtttct gctgggtgaa
    28321 ccgcgcgcgg tcgagctgtc atggcacggc aacttctgag tcgacgcggc ggggccatcg
    28381 ccgccacggc gctgctgctt gccgtggcca ccctgctgtg gtggctgcgc tacccggcgg
    28441 cctcagtcgc cgccagcgtt caacccatct cagcgccggc gggtgccggg gcgcgcctgc
    28501 cgcgactcgc cgcccatccc gccggtggcg cggtgctctc ctgggtcgag ccggcgccag
    28561 aaggtggcca tctgctgcgc gtggcacgct atcggaacgg ccgtttcgaa gcgccggtgg
    28621 aggtggcgcg cggccgtgac tggttcgtca actgggggga tttcccctcc gtggtaccca
    28681 tcgccgcgga tttctggctg gcccactggc tggtgcgcca tccgcgggcc gacagcccct
    28741 atcactacgg catcgccatg gccggttcca gcgacgatgg ccggcgctgg acgacccggc
    28801 cggcgccaca cgccgatgtc ggaccggccg agcacggctt tgccgccatc gccgcgctgg
    28861 atgaccgggc cgccatggtc tggctggacg gccgcgccaa cgatgcccgc cacagcttcg
    28921 cgctgcgcac cacggagatc gagcgcgacg gtgctgccgc cgccgagtcc atcatcgaca
    28981 gcgacgtctg ctcctgctgc tggccggcgc tggcgcgcac acggaaccag ctgtgggtgg
    29041 cctatcgtgg ccgcaccagc gacgagatcc gcgattttca actgcgctac cgcactgtcg
    29101 ctggctggtc ggcaccgatc gcgctcgccg gcgaaggttg gcgcatcgcc ggctgcccga
    29161 ccaacggcgt gagtctggca gcacaggatg aacggctggc agcggcctgg tacacggcgg
    29221 cggatggggt ggcacgggtg cgggtcgcct tcattcgcga tggcgggaac gccctggaaa
    29281 gcatccacga tgtcgacgtc gccacgccgg tgggacgcgc cgccatcgcc tggctggaca
    29341 gcgagcgggt ggcggcactg cacatcgccg cgtccagcag cgcgctggcc gacctgcggc
    29401 tcacactgct ctccgaccgc ggcatcgagc gcagcctgac gctgatacag gtgcctgcca
    29461 accgcgactc cggtgtgccg caactgctgg cagtcgatgg cgaactgctg gtcgcctgga
    29521 ccgatgccgc ccccggtcac ggggttcggg tgctgcgtgt tccgcagcgc gccttgcggt
    29581 aaatccggcc ctcgcgctca gggcaccggc gcggcctgcg atcgggcctg gcccggatcg
    29641 gtgacgaaac cgatgtgcag cacgccggcg cgggtggcgg cggccagcac ctgggccacg
    29701 gcatcgtagc gcaccgcgcg gtcggcctcg acatgcagct cggcagcggg ccgtgccgcc
    29761 agcgcacggg cgcgctcctc cagctcggcc aggctcagcg gctgaccgtc ccagtgcagt
    29821 tgtccatcgg cggcaatggc caccaccacc ggcttgctgg gcgtctgcac cggctggctc
    29881 ttcgcctccg gcagcttcac cttcaccgcc tgggtgatca gcggtgccgt gacgatgaag
    29941 atcaccagca gcaccagaat cacatccacc aacggcacga tgttgatctc ggcgctgctg
    30001 cgcgccgtag aggcaactcc gtctcccagc gccatggctc agctccgcag cggatgaatg
    30061 gctgcgcgct ctcttttgat cgcgccagaa ggcgagccgg gcagcgtggc gcgttgcagc
    30121 agggtggtgg cgaaatcttc ccgctgcgcc tgcaacagac ggtgacggcg ctgcagggtg
    30181 ttgtgggcaa agaccgccgg aatcgccacg gcgagaccca gtgcggtcat caccagcgcc
    30241 tcgccaatcg gtccggccac cgcggccagc cccgcctcgc cggtggcggc aatgcgcagc
    30301 agcgcatgat agatgcccca cacggtgccg agcagaccaa ggtaaggggc cgtcgatgcg
    30361 gtggtcgaca gcagtgccag gccacgctcg ccacgcaccg cagcacggtc aagctgctgg
    30421 cgcaatccac gggtcaggtg ggcggccagt ccgccggcct catcgaactg ctcgccggca
    30481 tcggcgagtc ggcggccatc gcgaaccacc gcaagcaggc tggcttcgaa cggtctgccg
    30541 aagggctctg gtggcgactg tgccagcccc gcagtttcga ggatcgtgcg catgtagcgc
    30601 tgactgcttc gaacctccag caggctgcgc agcagccaga gcagaaagaa ggtccagctc
    30661 gccagcgaca gcagtgccag aatggtcagc aggctccagc ccagcggatc gagctgctgg
    30721 acaaaatggg caaatccggt gtcgatcatc atcacctcgg cagggttcaa cgcaatgaaa
    30781 agtggatgcg ttgccgcgcc gtcgccgccg ctggcacgcc ggcgacccgg atcggatcac
    30841 actgccacag ccgcacggcc gcaacagccg cctgatcgag ccggccgaag cccgagggtc
    30901 ggctcacact cacctgtcgc acactgccat cggccgagag tgccaccagc aattcgacca
    30961 ccccctcttc gcccagacgc cgtgccgcca tcgggtattg aggcggagga cgccgtgggc
    31021 agtgcaccga cagctccggc ggttctgcat cggcagaaag cggggctgct gccggtggtg
    31081 gcgtcgcaac cgaaggcggg gccgggtcaa ccgctgccac ggcaggctcg gcaaccggtg
    31141 tgacgggtgc cggagcgggc tcgtcccgca gcgccaccgg agccagctcg cgcggctttc
    31201 tgtcgggtct gggtggtttt gtactggcga tctttgccgg tgcgggtgtc ggttccggca
    31261 tcgccgccga tggcggactg ggtgcaaccg gtggcgccgg cggcggagtc gggcgctcga
    31321 tcatccggat cgccaccggc tggatcgaca acgctggcgg cgccgggcgc tcccacaaca
    31381 gcaggacgac gccgccatgg agtgccactg caatcagcca tgccaatcca tgcagcgcag
    31441 gttgactcag tggcgaaggg tgctcaccac gccagcgatt ggatggctgg agtgcgcagg
    31501 taccgatcct gagaaaaagg ctgttcaagg tgccgccaac cctcggcaga gtcatgcccg
    31561 acatgcgtca acgcagcgag atccacgcac tccaatgcca aaacggccat catccttgag
    31621 tcgggccgtg acgggaatgc gacaaatcgt cgcaccgctt ccggctcgat ggtcacggcc
    31681 tcgtcaatgc cgaacgccgt tcacccctgc cacagtgcat tcaggttgct gaattgccac
    31741 ttctgcggat cttcgcggtt gacgatggtg tccgtctgcc cgctcttcga caggtcgagc
    31801 aattggaccg gtatcggcat gttggtgcgg gtcgagaaga acaccttcag cagcgggcgg
    31861 gtcaggctct gcggattctg ctcgacgatc acgccaaggc ggccggagtg cagcctgacc
    31921 aggctgccga gcggatagat gcccaccatc ttgacgaagg catggaagat ggcagtgtcg
    31981 aaatggccct cccacttcgc catgcgcgcc agagaatccg ccgcatccca accgttcttg
    32041 taaggccggt tcgaggtgat ggcatcatag acatcgcaga ccgcccccat ccgcgccaac
    32101 tggctgatct gctctccctt caacccatac ggatagccac tgccatcgat cttttcgtgg
    32161 tgatgcaggc agacatcgag cgcgctcggc gcggtgaatc cactccgggt cagaatcgcg
    32221 tggccacgct ccggatgcag ccggatgacg ctgaattcat ccgtagtcaa tgcgcccggc
    32281 ttgttcagta tcttctccgg catcgccatc ttgccgatgt cgtgcagcag accggccaca
    32341 cccgcctgct gggtctgctg ctcatccagc tgcatttgcc tcgccagcgc gatcatcagc
    32401 gcgcagaccg acaccgagtg catgtaggtg tactcatcct tgctcttcag ccgcgccagg
    32461 ctgatcaggg cggtggagtt tctcgccacc gagccactga tctcctcgac cagtggcagg
    32521 cactgctcca cttcgatcac cttgcccagc cgggcttcgg tgaacagcct ggcgaccgcc
    32581 tccttggagt ggtggatgat cccggatgcc cggcccagct cctcctgggc gctggttgtc
    32641 ttgttgagct ggatcgccgg ggcgaccggt tgtttgccgg caacggccac cgtctcggtc
    32701 gctgccggtt ccggggtcgg ctctgctgcc ggctctgcgg ccacatccga tgcgtccgtc
    32761 agtcctgcca catcccggcc cttgtcgcag tcgatccaca ccgccgagac gccacttttt
    32821 ttcagtgccc gcagatcggt cgggtcatcg agcacgaact tgctcttcca gaacggatgg
    32881 ctcagccaac tgccttcgag cccatggatg tacatgccga gacaaacctg ctcggtggtg
    32941 attttcttca gcatccgcct cgatctccct ggcgacggtc atacgaaggt tcgggaacga
    33001 tgaaccctgt catggcagag gtgcgcgcag tcacggcagt gaacgacccc gacgggcaga
    33061 agggttgaag gtataatggc cgaccttttg cgaaggacgg aattcaggcg tgacatcctg
    33121 ccggctggtt cgcaccagtt cggagcacca gcgccgctgc aggccggttc cgcctgtcga
    33181 aaagtataga cctggcttca gcaaccgcca ggcttcagaa ccccccagcg atctccaccg
    33241 gtgccgtttc atgaccgcct catctcgcca gattcttgtc accagcgcgc tgccctatgc
    33301 caatggaccg atccatctcg gccacatgct ggagtacatc cagaccgaca tctgggtgcg
    33361 ctttcagcgg ttgcagcaac agcgctgttt ctacgtctgc gccgacgatg cccacggcac
    33421 cgccatcatg ctcaaggccg aggagctggg catcacgccc gaggcattga tcgaacaggt
    33481 gagcgccgag catcagcgcg attttgccga cttcggcatc agtttcgaca actaccactc
    33541 cacccactcc gaagagaacc gccacttcgc cgagctgatc taccagcgct gccggcaggc
    33601 aggccacatc gaaacccgca ccatcagcca gttctacgac ccggacaagg agctgtttct
    33661 ggccgaccgc ttcatcaagg gcagctgccc acgctgtggc gcggccgatc agtatggcga
    33721 caactgcgag gcttgtggcg ccacctacag ccccgatcag ctgatcgagc cgcgcagtgc
    33781 gatctccggc gccacaccgg tgatgcgcag cagcgagcat cagttcttcc ggctggccga
    33841 cttcaccgag ttgctgaagc agtggacccg cagcggcgcc ctctccgagg aggtggccaa
    33901 caagctcgat gagtggctgg agggcgggct ggagtcctgg gacatcagcc gcgatgcacc
    33961 ctacttcggc tttcgcattc ccggcagcga cgacaagtac ttctacgtct ggctcgatgc
    34021 gccgatcggc tacatggcga gcttcaagaa cctctgcgac cgcacgcccg ggctcgattt
    34081 cgaccactac tggcgcccgg gcagcagcag cgagctctac cacttcatcg gcaaggacat
    34141 catcaccttc cattcgctgt tctggccggc gatgctgcat gccgcccggt tccgcacgcc
    34201 aacggcggtg catgtgcacg gtttcgtcac catcaacggg cagaagatgt cgaaatcgcg
    34261 cggcaccttc atcaaggcgc gcacctttct cgaccatctg gagccggaat atctgcgcta
    34321 ctacttcgcc gccaagctct cggcgcgcat cgatgacctc gacctcaact tcgacgactt
    34381 cgtgcagcgc atcaatgccg acctggtcgg caagctggtg aacatcgcca gccgctcggc
    34441 cggtttcatc cacaaacagc acgccgggcg actggctgcg caactgcccg agccggcact
    34501 gtgggatgaa ttcgtcgccg ccggcgagca gatcgccgaa cactacgagg cccgcgagtt
    34561 cgcccgggtg gtgcgcgagg tgatggcgct ggccgaccgc gccaaccagt acatcgatca
    34621 gcagaagccc tggctgctgg ccaaggatcc ggcccggcag ccacaggtgc agcagatcgc
    34681 cagcttcggg ctcaacctgt tccggctgct ggtgctctat ctgcaaccga tcctgccgcg
    34741 cctggccgag cgggtcgaag gctttctggc cacgccgctg ccgaactggt cccgacgcaa
    34801 cgcgccgctg ctcgaccatc tcattgcccc gttcaccccg ctgttcaccc gcatcgagcc
    34861 cgacaccatc acggcactgc tggccgccag tcagcagaac gcgaccgaga ctgcacccgt
    34921 gatcagcgca ccagaacccc cgggcggcga ggcgatcgcc gccaccatcg attacgacag
    34981 ctttgccaag gtcgacctgc gcatcgcccg catcgtcgcg gccgaaccgg tcgaaggggc
    35041 cgacaagctg ctgcgcctca ccctcgacct cggcggcgag cagcgcacgg tctttgccgg
    35101 catcaagagc gcctacaccg ccgagcgcct gatcggccgc ctgacggtga tggtggccaa
    35161 cctggcgccg cgcaagatgc ggttcggcct ctccgagggg atggtgctgg ccgccggtcc
    35221 cggtggcgag cagatctggc tgctgagtcc cgacagcggc gccctgcccg gcatgcgggt
    35281 gcgctaggca agggccgatg tcgcaaccgg gagtcgatgc ggtgccggca ctggtcgaag
    35341 ccatcgatgc gctgctgccg cagacccagt gtggccgctg cggccatccc ggctgtcgcc
    35401 cctatgccga ggccattgcc agtggcgagc ccttcaatcg ctgcccgccg ggcggacagc
    35461 tcaccatcga cctgctggcc accctgctcg agcgccccaa actgacgctg gatgaacgcc
    35521 acggcactga gcagccgccc caggtggcgc tgattcgcga ggaggagtgc atcggctgca
    35581 ccaagtgcat ccaggcctgc ccggtcgatg cgatcgtcgg cgccgcgcaa tgga
//
LOCUS       DAORCQ010000013        34256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_652_length_34256_cov_6.145025, whole genome shotgun sequence.
ACCESSION   DAORCQ010000013 DAORCQ010000000
VERSION     DAORCQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 34256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34256
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_652_length_34256_cov_6.145025"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1177)
                     /locus_tag="PKI23_02355"
     CDS             complement(248..1177)
                     /locus_tag="PKI23_02355"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNN35813.1"
                     /translation="MAETSFWHKVDKTLRHHQRRAQVWLRDAAWPLFRMRDCRPLFIV
                     GCSRAGTTLVYKTFSESVALGSLQRETHDFWAALHPPAAIGWKSHAVAAELATPEEAD
                     RIARHFYAETGHQRWVDKNNQGGLSIPYLLRLFPRAHFVYVKREPGDNVNSLIEGWMR
                     PDEFATWSQQLPGEVALEQGRLRQWCFFLAEGWEAYRHASVEAVCAFQYQAMNQAILT
                     ARDAVDPRQWSEIHYEALVDDPVGGFRTLFESCDLPFDAAMAHHCATVLKRPYNAFSP
                     IGVEKWRASRFADKVERVLPQLTEVAAAMGYPR"
     gene            complement(1170..2483)
                     /locus_tag="PKI23_02360"
     CDS             complement(1170..2483)
                     /locus_tag="PKI23_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HNN35814.1"
                     /translation="MSGFRDFFVLIVVMGSLPLILSRPFLGVLMWSWLSYLNPHKLSW
                     GFAREFPFAMLVALATLAALLLTREEKKVPVNGITILLLLFLFWMLITSVTAFFPELA
                     WRQFDKVWKIFLMTFVVMMLAVSRERIQALVAVMALSLAFYGVKGGIFTLTSGGSYAV
                     YGPGGTFIGGNNEIGLALIMTIPLLRYLQLQASARWAKQACLLSMLLCFVSVVGTQSR
                     GAFVAVLVMSLYLFFKSRGSLLLLVPMVAVGAFIYSFMPDSWHYRMSTIETYDQDASA
                     LGRINAWKMAFNLAQHRLTGGGLECFQGPVFGLYAPVPWDVHDAHSIYFEVLGEHGFV
                     GLALFLLLGLSGLLLAGRTIRLVGDDPQQRWLRDLAAMLQVALIGYAAAGLFLGLAYF
                     DFYYALLALIAVGHRIATGDLRVTGLWKLEQAGSVLARPGVAGHG"
     gene            complement(2480..3742)
                     /locus_tag="PKI23_02365"
     CDS             complement(2480..3742)
                     /locus_tag="PKI23_02365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN35815.1"
                     /translation="MSSDSRPHLLLFSTLFPSVVQPQAGLFIRERMFRVARELPLQVV
                     SPQPWFPGQGLIRRFRPGYRPQAPAIEQQQGITIHHPRFLALPAIGRRWDGLSMALCC
                     WPLLRRLRAAGQIDLIDAHFGYPDGYAAVLLGRWLKRPVTITLRGTEVPHAQGVQRNR
                     LRQAVTGATQLFTVSDSLRRLALELGAAPERLLVVPNGVDRACFHPLDRQAARAELGL
                     PAQARILITVGGLVERKGFHRVIAALPTLLQRHPQLHYLLVGGPCAEGNIEPQLREQV
                     KALGLEGRVHFLGALPPQRLRLPLSAADLFVLPTRNEGWANVLLEALACGLPVVASDV
                     GGNAEVINDPRLGTIVALEDQAALVAAIDAWLGCEVDHGYLANYVGRNGWEQRVSTLV
                     QQFRRLAGHPGNASATECLTAKQSEECE"
     gene            complement(3742..4938)
                     /locus_tag="PKI23_02370"
     CDS             complement(3742..4938)
                     /locus_tag="PKI23_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HNN35816.1"
                     /translation="MHILHILDHSIPLQSGYTFRTRNILRQQRALGWQTSHLTGPKQT
                     GCTLEREEVDGLLFHRTPATAAGGGWLPEPLAVIAALGRRLDQVIEELRPDLLHAHSP
                     ALDGIAALRAGRRHRLPVVYECRAFWEDAAVDHGTSREWGVRYRLTRLMESWVFRRAD
                     AITTICAGLRDEIIGRGIAAGKITVIPNAVDLAQFGSADEPDAALQAQLGLTGQRVLG
                     FVGSFYGYEGLDLAVAALAQIGDPAVKLLLVGGGPQEQALRAQVERLGLGQRVIFTGR
                     VPHQEVNRYYSLIDLLLYPRHSMRLTDLVTPLKPLEAMAQQKLLLASDVGGHRELIED
                     GRTGWLFPAGDAAALARRAEQILAQPDEWPKIRAAGRAFVEQERNWPASVARYRDLYA
                     RLLTAA"
     gene            complement(4946..6838)
                     /locus_tag="PKI23_02375"
     CDS             complement(4946..6838)
                     /locus_tag="PKI23_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HNN35817.1"
                     /translation="MCGIAGIVDLTDRREPDRALLERMNQSQFHRGPDEGGYHLEAGV
                     GLAHRRLSIIDLSAGQQPMHSADGERVVVFNGEIYNFIEVRKKLQALGQQFRTHSDTE
                     VILAGWAVWGEQCVDHFRGMFAFALWDRPRQTLFLARDRLGVKPLYYALIDGWLLFGS
                     ELKVLLCDPRLSRRLDPTAIEEYFALGYVPDPKCILSSVQKLPAGHTLCLQRGQGVPA
                     SREYWDIPFTPLPQASEAELAEELIERLREAVTIRMIAEVPLGAFLSGGVDSSAVVAM
                     MAQAQQEPVNTCSIGFDVAAFDESSYALQVAERYHTQHHVKRVDPNDFDLIDRLAALY
                     DEPFADSSALPTYRVCQLARERVTVALSGDGGDENFAGYRRYRWHMNEERLRGLLPRT
                     IRRPLFGTLGRLYPKLDWAPRYLRAQSTFQALARDPIEGYFHNFSILVDAQRDQLYSD
                     AFKRQLQGYRAVELFRHHAERAPTEDPLARVQYIDMKTYLVGDILTKVDRASMAHALE
                     VREPLLDHPLIEWASGLPADFKLRGQQGKYLLKRALEPHLSNEILYRDKMGFAVPLAN
                     WFRGPLKERLRRSLLEGVLADSGLFNGDSITQLVEQHQSGRRDHSAALWSLLMFESSL
                     RHLGVQ"
     gene            complement(6851..8044)
                     /locus_tag="PKI23_02380"
     CDS             complement(6851..8044)
                     /locus_tag="PKI23_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNN35818.1"
                     /translation="MAEATAPPLIAHVVHRFDIGGLENGVVNLINRLGSEYRHAVICL
                     TDYNPQFCRRIHHPAVSYHALHKRPGQDWAMLWQLGRLLRQLRPAVLHTRNLATLEAQ
                     AIGWLARVPARLHGEHGWDMADLHGKNRRYRMLRRLFVPLIDRYIALSVEGERYLHDE
                     IGVAAGKIERIINGVDAEQFQPLPRRRDTLLPAGFVPEQGVVIGSVGRLAEVKNHVAL
                     AHAFGQLLQQRPELRQRARLVIVGAGPQREAVQAILQKSGHAHALYLAGAQQNIAEWM
                     QSFDLFVLPSLAEGISNTILEAMACGLPVVATAVGGNRELVEPGVTGQLVPLDEPESM
                     VQAVLDYVEDAPLRARHGAAGRARIERELSLARMVERYRGLYRQLAFTDSTAAGLHAR
                     TDSTD"
     gene            complement(8037..9146)
                     /gene="rfbB"
                     /locus_tag="PKI23_02385"
     CDS             complement(8037..9146)
                     /gene="rfbB"
                     /locus_tag="PKI23_02385"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNN35819.1"
                     /translation="MNHRPRRLLVTGGAGFIGANFVHHWLAGHRDDRLLVLDALTYAG
                     NLRSLQPVADDPRLRFVKGSICDTELVEQLLREERIDTLVHFAAESHVDRSITGPDAF
                     IDTNVVGTHSLLKAARSVWLGQNRVEAHHFHHVSTDEVYGTLAPTDPPFAETTPYAPN
                     SPYSASKAASDHLVRAYHHTYGLRTTTSNCSNNYGPYHFPEKLIPLCLINILFGRPLP
                     IYGDGQQIRDWLYVTDHCRGIEAVIERGQLGETYNIGGNNEKSNLEVVTTLCTLLDQA
                     FAANPELAVRFPDAPAARGLASRTLITHVQDRAGHDRRYAIDARKIEQALQFRPLESF
                     ESGIARTVTWYLDHEAWWREVMDGSYRDWIQRQYG"
     gene            complement(9143..10009)
                     /gene="rfbD"
                     /locus_tag="PKI23_02390"
     CDS             complement(9143..10009)
                     /gene="rfbD"
                     /locus_tag="PKI23_02390"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNN35820.1"
                     /translation="MKVLLTGATGQLGHALIRSAPATVELVALDRAALDLARPEGLAA
                     LIEQHRPALLINAAAYTAVDRAEQEPELAQRINAQAVAALAAACSASAVRLMQISTDF
                     VFDGRQSRPYRTEDQPAPLGVYGASKQAAEALLLASSQLDWLIIRTAWVYASHGSNFV
                     KTMLRLMAERDSLGVVADQIGTPTHAATLARAVWAAAGQPALRGIHHYTDAGVASWYD
                     FAVAIMEEAVALGLLQRPLQIRPITTADYPTPARRPAYSVLDKQASWAAFGFTPDHWR
                     VELRTMLKELQR"
     gene            complement(10006..10554)
                     /gene="rfbC"
                     /locus_tag="PKI23_02395"
     CDS             complement(10006..10554)
                     /gene="rfbC"
                     /locus_tag="PKI23_02395"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016394179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNN35821.1"
                     /translation="MKFIPTEIAEVVLIEPQRFGDARGFFMETWQRRHFAAAGLDLDF
                     VQDNHSRSAQGVLRGLHYQLQQPQGKLVRVTLGEVYDVAVDIRRSSPTFGRWVGVLLS
                     AEQPRMLWVPPGFAHGFYVLSDVAEFVYKCTDYYAPQHERSLRWDDPDLAIDWPLLPE
                     QPPLLSAKDQQGQWLVDAELDP"
     gene            complement(10551..11459)
                     /gene="rfbA"
                     /locus_tag="PKI23_02400"
     CDS             complement(10551..11459)
                     /gene="rfbA"
                     /locus_tag="PKI23_02400"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNN35822.1"
                     /translation="MSNPVTHPPKGIILAGGSGTRLHPLTLTVSKQLMPVYDKPMIYY
                     PLATLMLAGIRDILIITTPADQAAFQRLLGDGANWGINLSYAVQPSPDGLAQAFIIGE
                     SFIGRSPVCLILGDNIFYGEGLSKKLQTAASRSEGATVFGYYVQDPERYGVVEFDTAG
                     RVVSLEEKPQQPKSHYAVTGLYFYDNQVVEIAKAVRPSPRGELEITDVNQTYLERNRL
                     TVELFSRGTAWLDTGTHNSLLDAAHFIRVVEERQGLKIACPEEIAYRMGFIDAAQLAR
                     LAEPLKKSGYGSYLLGLLSRQQEPAP"
     gene            complement(11456..12994)
                     /gene="xrtA"
                     /locus_tag="PKI23_02405"
     CDS             complement(11456..12994)
                     /gene="xrtA"
                     /locus_tag="PKI23_02405"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HNN35823.1"
                     /translation="MATDPLQPILAWRWPLLRLALLLTLLFAGFWPTFSSMVQIWARS
                     ETFTHAFLILPITLYLIWLRRGELAALTPGTSRWPWLLLLPTGLGWFLARLVDVAVVE
                     QAAWVAMLPLAVWAVLGSAVVWRLLFPLGYLFFMVPFGEGFIPPLMQFTAFFTINAVK
                     LTGIPIFAEGTFFTLPSGNWSVVEGCSGVRYLIASIALGALFSYINFRSTGRRLLFML
                     LFIVVPIVANGLRAFMIVMIAHYSDMKLALGVDHYIYGWVFFGLVMFILFAIGMVWSE
                     PPAHRAPAPGRSRSTVGATASAWSTLLVTALLIGGWPLLEQWSEQSTTVPTAAAPSLP
                     ERLGDWRRLDGTPPPDWKPHYQGMAQEQLGWYRQQGQWVGVYLAYYPLQRQDAELINS
                     QNVLIPQKHPLWHQPVDRLIEAEALPGLTRLREGRLDGPQQLLVWSWNRIGPTQTTDP
                     YWGKLLTAYNRLILGDTRAVGVVIFTPYSETDSVADRQRLQAVAQFLTETLNQQSQME
                     KPAR"
     gene            complement(12984..14216)
                     /locus_tag="PKI23_02410"
     CDS             complement(12984..14216)
                     /locus_tag="PKI23_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNN35824.1"
                     /translation="MTREPLLFLAHRIPYPPDKGDKVRSYHFLRHLAERYRLFLGTFI
                     DDAADLPHRARLEALCADSCFIRLRRGTLPVAAARGLLRGAPLTNFHYRSATLRRWVE
                     ATLRREPIGKAFLFSSPMAQYLTAARHRGLHRVIDFVDVDSDKWRQYSERHRPPLRWL
                     YQREQRTLLRFERGIARQFQASLFVSEHEATLFRGFAPESAERVLSVPNGVDLDYFAP
                     DASLPSPYAPQEQVLVFTGAMDYWANIDAVGWFAQAIFPRLKARHPALRFYIVGSNPA
                     PQVLALARTPDVVVTGRVADVRPYLQHARLAVAPLRVARGIQNKVLEAMAMGRPIVVS
                     TAALEGITPQPWMQEIVAEGAEAMAERIDRLLTSERLDSLGALGRQTMLSHYSWQNSY
                     HLLDSVIENRAPRSLHGH"
     gene            complement(14220..15254)
                     /locus_tag="PKI23_02415"
     CDS             complement(14220..15254)
                     /locus_tag="PKI23_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNN35825.1"
                     /translation="MTTDHAIHPLDVSNRARWDAFVRARPEGTFFHLSGWQQVLEQAF
                     GHRTHFLYLEQQGQIEAVLPLAEVKSRLFGHSLVSTPFCVYGGPIATAPHHRDALLDA
                     ATRLATELGVDHLELRSMSRQAPDWPCKELYVTFRKEISADDEQNLKAIPRKQRAVVR
                     KGIDAGLHSEIDTGIDRFFHAYSSSVRNLGTPVFSRKYFRVLREVFGDECEILTITRQ
                     GETVASVMSFYFRDQVLPYYGGGTEAARACKANDFMYWALMCHAAGRGIRLFDYGRSK
                     IGTGAYDFKKNWGFTPEPLYYEYHLVKGQALPDLNPLNPKYRLLINTWQRLPLWLSQW
                     IGPWVSRYLG"
     gene            complement(15320..16177)
                     /locus_tag="PKI23_02420"
     CDS             complement(15320..16177)
                     /locus_tag="PKI23_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNN35826.1"
                     /translation="MKQGESVAPVRQTNAMTLDVEDFFQVAAFEGLIDPARWDSYPLR
                     VDQNTRRVMALFAERGIKATFFLLGWVAERLPQLVRDIAAEGHELANHGYAHARVNSQ
                     SREAFRSDIRHAKALIEDLSGMAVHGYRAPSYSIDVTTPWAHDEIAEAGHRYSSSVYP
                     VRHDLYGMPDAPRFPYRCSSGLTEIPITTLRLGGRNWPIGGGGYFRLLPYQLYRQALL
                     RFNQQEKSPGLFYFHPWEIDPDQPRPAGAGFRSRFRHYLNLERMESRVSRLLGDFHWD
                     RMDRVFLSE"
     gene            complement(16174..17313)
                     /gene="wecB"
                     /locus_tag="PKI23_02425"
     CDS             complement(16174..17313)
                     /gene="wecB"
                     /locus_tag="PKI23_02425"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNN35827.1"
                     /translation="MSAPLLCVVGARPNFMKIAPIMAAWRARAPQRPALLLHTGQHYD
                     ESMKRQFFEQLQIPQPDIDLGVGSGSHAVQTAQIMLQFEPVLERVRPAAILVVGDVNS
                     TVACALTAIKRGIPVVHVEAGLRSGDRSMPEEINRLITDQLSARLYLTERSAFDNLLR
                     EGIESSRCRFVGNVMIDTLHHHSRAALPLAQIPALAQPQRQVAARGAGHGVVTLHRPA
                     NVDDPQVLRRLLETLNQIGRRLPLIFPLHPRTRAQIVQQRLESLLDGETLLPVEPLGY
                     LEMLGLMRDARLVLTDSGGIQEETTALGVPCLTLRENTERPITLTEGTNTLVGRDPAT
                     ILAQVEEILASGGKRGRAPELWDGHAAERIVDDLLAWLDRGDLPL"
     gene            complement(17324..18370)
                     /locus_tag="PKI23_02430"
     CDS             complement(17324..18370)
                     /locus_tag="PKI23_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HNN35828.1"
                     /translation="MYEEFYQFSAKPFQVSPDPRFFYGSKAHKRAMSYLRYGLSQGEG
                     FVVITGGVGTGKTTLIRNLFADLGYDDVIAAQLVTSNLQADEMLKMISTAFNLPTEGL
                     TKAALLHQFEAFLRQADREGRRVLLVVDEAQNLPPATVEELRMLSNFQIENRPLLQSF
                     LLGQEEFRHIVQSPNMEQLRQRIIASCHLTPLDQLETRAYIEHRLKLVGWQGDNPEIT
                     DAAFDEIHRHTQGVPRRINSFCDRLLLYGYLEELKTLSSEAVAVVERELAQENGSNRP
                     LPMTRSPVRDDADDDEFESGSESQLPRTAPLSMSELDKRIHDIEYVIEVLEKNFNKKI
                     SLLRSVVHALKSSL"
     gene            complement(18381..19991)
                     /locus_tag="PKI23_02435"
     CDS             complement(18381..19991)
                     /locus_tag="PKI23_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HNN35829.1"
                     /translation="MHRGWYRLGGALLLVIGLPCHAVSWRAAPSVNVDLTYTDNIFLE
                     KNDRDSDTILIARPGFDLERNSGRSKLNLGYRLTQLTHLQQSGLDNTYHSLNGAGQWE
                     AIKQHLFLDAGVLASQELIDPTRSVNADLLGNEQNITTAWRGYFQPRWTQRLGGWGSY
                     EVGYGVDYVDYSGGSGNLNQSQGSTLTFNLTSGSAGMPLFWSANYLDNNIDYANSQQD
                     GGQQNGDATLGVWAGKRLRFSGTAGYSEYSYTPNSTLVADNRQPNNHYYRATIGWVPS
                     RKSQFDLFYQVREYDDDTLSETENKGSFGGRASWDPSRRWHLEGGYNEDFYGPAYDFS
                     LTHRLRRSSFSLKYEEEVTNTRQNLLQNQQIGTLICPSGTTFNPAQCVFVNPNQPVQL
                     GINQQLIGLFLPVGVIADDQFLEKRYTATWGYQRGRSAFSLNGYQVDRTYLTSSNESQ
                     DRGANFDWTLSLSGRTSLTTGVSWLTRDFMTGEKLDYDEFHLGLTQTVTRDASLSATY
                     RRISNDAQGSLLNDYRENRLTLSGNMRF"
     gene            complement(19996..20913)
                     /locus_tag="PKI23_02440"
     CDS             complement(19996..20913)
                     /locus_tag="PKI23_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HNN35830.1"
                     /translation="MDTIEKAFKKLQERRGEQSEARRRPGSGAVVRLPDEPPHSVTAQ
                     AMPEIDPEAQSRPYCEVKSSRYVEIDFDHLREGGLIIPDDERTRLKEEYRHIKRPLLK
                     KVAGLPDKQRNHRNIIMVTSARPGEGKTFNAINLALSIAEERNHRVLLIDADVLKSTV
                     AKKLGVEEGAGLVDYLTGDSDTPGELMYKTNIPQFTLMQAGKRHHLTTELLASKRMEQ
                     LVHELATRYADRIIVIDSPPLLSTTEASVLAQLAGQVVLVVAEGLTPQADVKKSLSLM
                     GTSVDVGLILNKSHHASDGYGYYYYGYGQ"
     gene            complement(20971..22530)
                     /locus_tag="PKI23_02445"
     CDS             complement(20971..22530)
                     /locus_tag="PKI23_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNN35831.1"
                     /translation="MNEQIELVLSYLRDILRRKWWILGSTWLVCAIGWVVVQRLPDQY
                     EASARVYVDTKSLLKPLLRGLTIEMDVNQQVRLMERTLLSRPNLEKIIRMADLDLSAG
                     ADEEFEALIASVGNRIHMKRDSEENLYSINYPDSDPERAKRVVQAVVTTFVENTIGEG
                     KGDGAAASAQRFLDKQIEEYEARLRESEDRLKEFKQKNVAFMSGSGGYFGELKSATEQ
                     LEAARLQVKEMQQRRDALREQVQASASSSGGDDELLLPTPGAGSGSTQYDGRIARLNE
                     QLDSLLLKYTENHPSVVEIRNTLKNLEELRQKDLQTQAQQPQSEFSSTPDPLKQQLKL
                     AFSQAEAELASLNARVQSYEQKVRDLQNRVDTVPQIEADLANLNRDYGIAKQKYEEFL
                     GRRESALISQRVEEDANDIQFRVIDPPRVPTAPAGPNRLLLMSATLGGGLLLGLGVAT
                     LFAVLRPTFTSTLLLAQMTNLQVLGTVSFVASSTLRKRHRLESILFVAGALLLVAVYA
                     ALASYYAFIRG"
     gene            complement(22614..23252)
                     /locus_tag="PKI23_02450"
     CDS             complement(22614..23252)
                     /locus_tag="PKI23_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNN35832.1"
                     /translation="MNNRVHFVAGLICATMLAGCGTLNSGQQTLPPATLHPSFNADRA
                     DYDYLIGPGDSLQIFVWNNPEVSMSVTVRPDGKISTPLMEDLPVTGKTPTQMARQVEV
                     ALAKYVKDPIVTIMVGGFNGPYSEQIRIIGQAARPQSLPYKQHMTLLDVMIAVGGVTD
                     FADANRVQLARIVNGQQEVYRVRLDDLVNGGDISANVDMRPGDILIIPEAWF"
     gene            complement(23437..24165)
                     /locus_tag="PKI23_02455"
     CDS             complement(23437..24165)
                     /locus_tag="PKI23_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNN35833.1"
                     /translation="MLFDDHYQVFLANGVHSEDLHFAVRYRVYCEERGFEDRQQFPEG
                     LEHDRWDPFSFKFIVRCRLSGEYVAAMRLVRSSSGQLPLHSHCQVAPGWWTQLPGQSI
                     VELSRLCVLRSHSGNFWDMKRAAKGAVYEPGIFWGMVRAAFSVSQDEEIGQWYFLTTR
                     ALARRLQQCMIHTDQVGEPCELNGTRHPYLVDVERSRQRIMTESPQLRRFYYSEPFCR
                     RLGDLDPQPQKSLHRPATQPAIML"
     gene            24403..25200
                     /locus_tag="PKI23_02460"
     CDS             24403..25200
                     /locus_tag="PKI23_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNN35834.1"
                     /translation="MINFSTPPAVAEMVCTLAPEHPARLFWSHFTPLYADSEALRDQV
                     FRIRHEVFCEEFGFEPLSADQRERDEFDPIAHHFLLGHNQSQQFAGCVRLIVPRPEAN
                     QSMLPIARFCWPHLDPYVQMLLQSRARCGEISRLAVRARYRRRQSDPHTPEGGALQAE
                     AAERSADRRLFPFVSFGIYLCIGAALELLHQRGQLDAALIMMEPKLYRNLKQQGFNFQ
                     PAGPVIDYHGRRAPFVITKQTLAPRLSSEMQLLKTSIAVALAPVIQP"
     gene            25222..27585
                     /locus_tag="PKI23_02465"
     CDS             25222..27585
                     /locus_tag="PKI23_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNN35835.1"
                     /translation="MNINLTRKILQHRTFSIVSMLLLIVFGSYGIRFLSLTVDYREYF
                     SATDPQLAAYNRIQADYSNNDNILIAIRARQGDLFNRPLLALIEALTTAGWSLPYSHR
                     VDSITNFQYSRAEGDELVVEPLFAHLDRMNEATIAQRRQVALQEPRLRGLLLATDAQA
                     TAVNISFTLPHQDDLAETAAAVGATRALVERFARQHPEVEFHLGGQLITNQAFPEATS
                     QDTTRLFPLVLLVMTLMIALAIPHLWSVLLVVLLMLFSVTVALGTVGWFGVRLTPDSI
                     AGSAMIMPIAIANAVHLLLAYFHRLHKGVQPLEAMAQSITANFMPMFLTAFTTVVGFL
                     AVNFSDSPPYRLMSNIVAIGQTVTFILTFLLLAPVATLMQRRVEIRSVQDRLDPLMCW
                     LSRCTTRHQKALLLFMGISTVLAIPLTMANRVSDNPLDWFSEQVPFRQDVDWINRHLS
                     GVTFIDYPLAAGGDMEITEPAFLQKLDAFTSWLRSQPRVRHVASISDTFKQLNQEMNQ
                     GEAVHYRIPESRELASQYLLLYEMSLPFGLDLTNQLNFNKSSTRVSVALDKLSTVETI
                     AFDQQVQQWMQANLPPQMRSQGSGLSVMLAHMTDRNTRYMLYGAVVAKLLMGLTLLLA
                     FRSWKISLVGLVPNMVPAFYAFAIWWFCVAEVGLALSMVLSMTFGIIVDNTIHLIDKY
                     DQARRTQGHAHAEATLYSYREAGPGVVVSSLSLCCGFAVLMVSDFTQNAQLGLMTTLI
                     MLLALGTNLLFLPPLLACFTPARWRHLQSAQQASGMQGQESDSPSGA"
     gene            complement(27555..29180)
                     /locus_tag="PKI23_02470"
     CDS             complement(27555..29180)
                     /locus_tag="PKI23_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNN35836.1"
                     /translation="MQHPRLSADLQAQLVAVVGAEHLLLDEQIHGYLADTLGSRRQLL
                     GVVRPASTGQVQAVVRLAGDHGLPLYPISSGRNWGYGSALPVTDGCLLVDLSRMKRIL
                     HLDPDLALLTVEPGVTQGDLWRHLQQQPRRFLVPVTGAGPDCSLIGNALERGYGITPV
                     ADHCAAIQSLEAVLPDGTLYRGALATLGHDEIEGCFKWGIGPHLDGLFNQSNLGIVTR
                     MTFQLVPEPQSMLALYFWLDDDQKLAAAVQAIQQLLHRLPGLLGGVNLMNSRRLLSMM
                     EPYPHEQIAPDGIMSDALVQQLARRNRVSAWMGFGALYGEKPLLRAARKVIARQLKGV
                     ASRTLFLAPSTIHRLLPWTQRLPGAPGRHLAGLVETLDRSLRVVAGEPSEIALPLAYW
                     RSRQPLPAGDYHPARDGCGLIWYAPLVPMRPEAVARYREMVERICPEHGIEPLITFTS
                     ISPRCFDSTVPILFDRSDAVATARARACYDALLAAGRQQGFFPYRLHVDAMSALIDAD
                     TPHWRMALKIKQALDPQGIIAPGRYAPLGESDS"
     gene            complement(29221..30135)
                     /locus_tag="PKI23_02475"
     CDS             complement(29221..30135)
                     /locus_tag="PKI23_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNN35837.1"
                     /translation="MELAFHTTDVFTNHPFGGAQVAVFPVADGLEPEQMQQIASEMNL
                     SETLFVLSTDTPQQRRVRIFTPDREVDFAGHTVLAAGHVLGSIGAIPLQEGDTRLDLL
                     HNFGPVEVHLTVSQSQPNFVQFTHTVHPVVDRFVPTDEELAAILSLERRAIGLDKFRP
                     LLVSCDHPYLIVPLRSLDALQQARFNHGAWAESLAPSMLAQQILLFCRQTESSEANFH
                     ARLVGPQVGPHDDPPVGAALPAFAAYLAEQPSTRQGTYTFTVERGFDATRKSLLHAEM
                     DKKPGKGLKLRVGGSVVSVTSGTLHLPG"
     gene            complement(30283..31134)
                     /locus_tag="PKI23_02480"
     CDS             complement(30283..31134)
                     /locus_tag="PKI23_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN35838.1"
                     /translation="MEVWKIGAATITRVVDLVSRIPATALVPDATLENLAPLMHWLKP
                     HFLNDDGTFALSIHTFLIESCGARILVDTCIGNHKPRGLPDWNMREGRYLEELASLGA
                     PRESIDRVLCTHLHVDHVGWNTMKSGTQWLPTFPNARYLIGEAEWGYWQNEEDPFGKE
                     ARADSILPIFAAGLVDLVDANHRINDEVCLIPTPGHTPGHVSVMVTSQGARAVITGDM
                     LHHPLQVARPQWRDIADVDPDQARATRHEFLNRFSDGATLVLGTHFATPTAGRIVRDG
                     DSYRFEV"
     gene            31336..32103
                     /locus_tag="PKI23_02485"
     CDS             31336..32103
                     /locus_tag="PKI23_02485"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNN35839.1"
                     /translation="MTRLADKFALVTGGASGLGRAQSLTMAREGAAVAVTDLNIAGAE
                     AVAAEIRAQGGRAIALPHDVADESSWQEVMGQTLNQFGRLSILVNNAGIAASVDAEEG
                     SLADWRHLMSINLDGVFLGVKHGIRALKQSGGGSIINISSIMGLIGSPGSGAYNASKG
                     GVRLLTKSAALYCAKAGYGIRVNSVHPGFIDTPMIQRIMQGPQAEAARQNLIALHPIG
                     RLGVPQDIANGVLFLASDESAFVTGSELVIDGGYTAQ"
     gene            complement(32181..>34256)
                     /gene="katG"
                     /locus_tag="PKI23_02490"
     CDS             complement(32181..>34256)
                     /gene="katG"
                     /locus_tag="PKI23_02490"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNN35840.1"
                     /translation="LNLKILSQHSAKSDPMDAGFDYAAEFNQLDYAALKADLKALMTD
                     SQDWWPADWGHYGGLFIRMAWHSAGTYRIADGRGGAGTGNQRFAPLNSWPDNGNLDKA
                     RRLLWPIKRKYGKRISWADLMILAGNVALESMGFKTFGFGGGREDIWEPEEDVYWGAE
                     AEWLATSDKPNSRYSGNRELENPLAAVQMGLIYVNPEGPDGNPDPVASGRDVRETFAR
                     MAMNDEETVALVAGGHTFGKAHGAGDPKLVGAEPEGAAIEEMGLGWRNALGTGKGSDT
                     TTSGIEGAWKPNPTRWDNGYFDMLFGYEWELTKSPAGAHQWTPKNCKPEHLIPDAHDP
                     SKKHPPMMTTADLSLRFDPIYEPIARRFWQNPDQFADAFARAWFKLTHRDMGPLERYL
                     GPEVPSEVLIWQDPVPAGRRDYDIEAVKARIAASGLGVSELVATAWDSARTFRGSDKR
                     GGANGARIRLAPQKEWEANEPARLARVLGVLEGIAAATGASVADVIVLGGNVGIEQAA
                     KAAGFDLTVPFAPGRGDATAEQTDVESFAVLEPAADGFRNYMKAGVTSPAEELLLDRA
                     QLLTLTAPEMTVLVGGLRALGAVHGGSRHGLLTTRPGVLTNDFFVNLLDMGVEWKAAG
                     AGLYEAHDRATGAVKWTGTRADLVFGSNAELRAIAEVYASDDAAGKFVRDFVAAWVKV
                     MNADRFDLG"
BASE COUNT         6150 a        11051 c        11066 g         5989 t
ORIGIN      
        1 catgcacgct gttaaagccg tggctctgcc ccagaaaata ggctggagtc ttggagctac
       61 agccgatacc gctcagctta attacctgac tttgattcag tcccaattcc caagcagcgt
      121 tgatgatgcg cgagctgatg gaatcgtgac cacagccatt gcaaagtgtg gagtttgacc
      181 cctggtagac ctttttatca agacccaaag cgttgacctt cggcgcacga gcagccgcag
      241 ccgccactca ccgcggataa cccatggcag cggccacctc ggtcagttgc ggcaacaccc
      301 gctcgacctt gtcggcaaag cggctggcgc gccacttctc gacgccgatc ggtgaaaagg
      361 cgttgtaggg ccgcttcagc accgtggcgc agtggtgggc catcgccgca tcgaatggca
      421 ggtcgcagga ttcgaacagc gtgcgaaagc ccccgaccgg gtcgtcgacc agtgcctcgt
      481 agtggatctc gctccattgg cgcggatcga ccgcatcacg cgcggtcaga atcgcctggt
      541 tcatcgcctg atactggaag gcgcagaccg cctcgacgct ggcatggcgg taggcctccc
      601 acccctcggc cagaaaaaag caccactgcc gcaatcggcc ctgctccagc gccacctcgc
      661 cgggcagctg ttgcgaccag gtggcaaact catcgggccg catccagccc tcgatcagcg
      721 agttgacgtt gtcgcccggc tcccgcttga cgtagacgaa gtgggcgcgg gggaacagcc
      781 gcagcagata ggggatcgac agcccaccct ggttgttctt gtcgacccag cgctgatggc
      841 cggtttcggc gtagaaatgg cgggcaatcc ggtctgcttc ttccggcgtg gccagttcgg
      901 cggcgacggc atggctcttc cagccgatgg cggccggtgg atggagcgcc gcccagaagt
      961 catgggtctc gcgttgcagc gaacccagcg ccaccgattc ggaaaaagtc ttgtagacca
     1021 gcgtggtgcc ggcgcggctg cagccgacga tgaagagcgg tcggcagtct cgcatcctga
     1081 acagcggcca ggcggcatca cgcagccaca cctgcgcgcg tcgctggtgg tggcgcaggg
     1141 tcttgtcgac cttgtgccag aaagaggtct cagccatggc ccgccacccc cggtcgtgca
     1201 agcacactgc cggcctgctc cagcttccac aaaccagtca cccgcagatc gccggtggcg
     1261 atgcggtgac ccaccgcgat cagggccagc agcgcatagt agaagtcgaa ataggccagc
     1321 cccagaaaga gtccggcggc cgcatagccg atcagcgcca cctgcagcat ggcggccagg
     1381 tcgcgcagcc agcgctgctg cggatcatct cccaccagcc ggatggtgcg cccggccagc
     1441 agcagccccg acagccccag cagcaggaac agggcaaggc cgacaaagcc atgctcaccc
     1501 agcacctcga aatagatgct gtgggcatcg tggacatccc agggcaccgg agcgtaaagg
     1561 ccaaagaccg gcccctggaa gcattccaat ccgccacccg tgagccgatg ctgtgccaaa
     1621 ttgaaggcca tcttccaggc gttgatccgc cccagcgccg aggcatcctg gtcataggtt
     1681 tcgatcgtgc tcatccgata gtgccaactg tccggcatga agctgtagat gaaggcgccg
     1741 acggccacca tcggcaccag cagcagcagc gagccgcgac ttttgaagaa cagatagagg
     1801 ctcatcacca gcacggcgac aaaggcgccg cgcgactgag tccccaccac tgagacaaag
     1861 cagagcaaca tgctcaacag gcaagcctgc ttggcccagc gtgcactggc ctgcaactgc
     1921 aggtagcgca gcagcggaat ggtcatgatc agcgccaggc caatctcgtt gttgccaccg
     1981 atgaaggtgc cacccgggcc atagactgca tagctgccac cgctggtcag cgtgaagatg
     2041 ccgcccttga cgccgtagaa ggcgagcgac agcgccatca ccgccaccag cgcctggatc
     2101 cgttcgcggc tgaccgccag catcatcacc acgaaagtca tcagaaaaat tttccacacc
     2161 ttgtcgaact gccgccaggc cagttcagga aaaaaggccg tgacgctggt gatcagcatc
     2221 cagaacagga acagcagcag caggatggtg atgccattga ccggcacctt cttctcttcg
     2281 cgggtcagca gcagcgcggc cagcgtggcc agcgccacca gcatcgcgaa cgggaactcg
     2341 cgcgcgaacc cccagctcag cttgtgcggg ttgagatagc tgagccacga ccacatcagc
     2401 acaccgagaa aggggcggct caggatcagc ggcaggctgc ccatcaccac gatcagcacg
     2461 aagaaatcac gaaagccgct cattcgcact cctcactctg tttcgcggtc aggcattcgg
     2521 tcgccgaggc gttgcctggg tgaccggcca gccggcggaa ctgctgtacc aatgtgctga
     2581 cccgctgttc ccagccgttg cggcccacat agttggccag atagccatgg tcgacctcgc
     2641 atcccagcca cgcgtcgatc gccgcgacca gcgccgcctg atcctccagc gcgacgatgg
     2701 tgcccaggcg cggatcattg atcacctcgg cattgccgcc gacatcgctg gccaccaccg
     2761 gcaggccgca ggccagtgcc tcgagcagca cattggccca gccctcgttg cgggtcggca
     2821 gcacgaacag gtcggccgcc gacagcggca gccgcagccg ctgcggtggc agggccccga
     2881 gaaagtgaac ccgcccctcg agcccgagcg ccttcacctg ctcgcgcagt tgcggctcga
     2941 tgttgccctc ggcgcaggga ccgcccacca gcaggtagtg cagttgcgga tggcgctgca
     3001 gcagggtcgg cagcgccgcg atgacccgat gaaaaccctt gcgctcgacc agaccaccga
     3061 cggtgatcag gatccgcgcc tgcgccggca agccgagttc ggcacgggcg gcctggcggt
     3121 cgagcggatg gaagcacgcc cgatcgacgc cattgggcac caccagcagc cgttccggcg
     3181 ccgcgcccag ctccagcgcc agccggcgca atgaatcgga gacggtaaag agctgggtcg
     3241 cgccagtcac tgcctgacgc aaccggttgc gctgcacacc ctgggcatgt ggcacctcgg
     3301 tgccgcgcag ggtgatggtg accggtcgct tcagccagcg gccgagcagc accgccgcat
     3361 agccatcggg gtagccgaag tgggcatcga tcaggtcgat ctgcccggcg gcgcgcagcc
     3421 gacgcagcag cggccagcag cagagcgcca tcgacaggcc atcccagcgc cggccgatcg
     3481 ccggcagcgc cagaaagcgc ggatggtgga tggtgatgcc ctgctgctgc tcgatggcgg
     3541 gcgcctgcgg ccggtagccg ggccggaacc ggcgaatcag cccctgaccg ggaaaccacg
     3601 gctgcgggct gaccacctgc agcggcagtt cgcgggccac ccggaacatc cgttcgcgga
     3661 tgaacagtcc ggcctgcggc tgcaccacgc tgggaaacag cgtgctgaac agcagcagat
     3721 ggggccggct gtcgctggac atcaggctgc cgtcagcagt cgggcataga ggtcgcggta
     3781 gcgcgccacg ctggcgggcc agttgcgctc ctgctcgaca aaggcgcggc cggcggcgcg
     3841 gatcttcggc cattcatcgg gctgggccag aatctgctcg gcgcgccggg ccagcgcggc
     3901 ggcatcgccg gcgggaaaga gccagccggt gcggccatcc tcgatcagct cgcggtggcc
     3961 accgacatcg gaggccagca gcagcttctg ctgcgccatc gcctccagcg gcttcagcgg
     4021 cgtcaccaga tcggtcagcc gcatcgaatg gcgcggatag agcagcaggt cgatcaggct
     4081 gtagtagcgg ttgacctcct ggtgcggcac ccggccggtg aagatcaccc gctggccgag
     4141 gccgagccgc tcgacctgcg cccgcagcgc ctgctcctgc ggtccgccac cgaccagcag
     4201 cagcttcacc gccgggtcac cgatctgtgc cagtgccgcc accgccagat cgagcccctc
     4261 gtagccatag aaggagccga cgaaaccgag cacccgctgg ccggtcagcc ccagttgcgc
     4321 ctgcaacgcc gcatcgggct cgtccgcgct gccgaactgg gccagatcga ccgcgttggg
     4381 gatcaccgtg atcttcccgg cggcgatgcc gcggccgatg atctcgtcac gcagaccggc
     4441 acagatggtg gtgatggcat cggcacggcg aaacacccag ctctccatca gccgggtcag
     4501 ccggtagcgc acaccccatt cacggctggt gccatggtcg acggcagcat cctcccagaa
     4561 ggcgcggcac tcatagacca ccggcagccg atggcggcgg ccggcacgca gcgcggcaat
     4621 gccatcgagc gccggtgaat gggcatgcag caggtcgggg cgcagctcct cgatcacctg
     4681 atcgagtcgc cggccgagcg ccgcgatcac cgccagcggt tcgggcagcc agccaccgcc
     4741 cgccgcagtg gctggcgtgc ggtggaacag caggccatcg acctcctcac gctccagcgt
     4801 gcagccggtc tgcttgggcc cagtgaggtg cgaggtctgc cagccgagcg cgcgctgctg
     4861 cctcaggatg ttgcgggtgc ggaaggtgta gccgctctgc agcggaatcg aatggtcgag
     4921 gatgtgcagg atgtgcatgg ccgactcact gcacgccgag gtgacgcagt gaggattcga
     4981 acatcagcag cgaccagagt gcggcactgt ggtcgcgccg ccccgactga tgctgttcga
     5041 ccagttgggt gatgctgtcg ccgttgaaca ggccgctgtc ggccagcacc ccctccagca
     5101 gcgaacggcg cagccgctcc ttgagcggac cacggaacca gttggccagc ggcaccgcaa
     5161 agcccatctt gtcgcggtag agaatctcgt tggagagatg cggctccagc gcccgcttca
     5221 gcaggtattt gccctgctgg ccccgcagct tgaaatccgc gggcagaccc gaggcccact
     5281 cgatcagcgg atggtcgagc agcggctcac gcacctccag cgcatgggcc atgctggcac
     5341 ggtcgacctt ggtgaggatg tcgccgacca gataggtctt catgtcgatg tactgcacac
     5401 gcgccagcgg atcctcggtg ggggcccgct ctgcatggtg acggaacagt tcgacggcac
     5461 ggtagccctg cagctgccgc ttgaaggcat cgctgtagag ctgatcacgc tgcgcatcga
     5521 ccagaatcga gaagttgtgg aaatagccct cgatcgggtc gcgcgccagc gcctgaaagg
     5581 tgctctgggc acgcagatag cgtggcgccc agtcgagctt gggatagaga cggccgaggg
     5641 tgccgaacag cggacggcgg atggtgcgcg gcagcagtcc gcgcagccgc tcctcattca
     5701 tgtgccagcg gtagcggcgg taaccggcga agttctcatc gccgccatca ccggagagcg
     5761 cgaccgtcac ccgttcgcgc gccagttggc agacgcggta ggtcggcaat gcggagctgt
     5821 cggcgaaggg ttcgtcatag agcgccgcca gccggtcgat cagatcgaaa tcgttgggat
     5881 cgacccgttt gacatggtgc tgggtgtggt agcgctctgc cacctgcagc gcatagctcg
     5941 actcgtcgaa ggccgcgaca tcgaaaccga tcgagcaggt gttgaccggc tcctgctgcg
     6001 cctgggccat catcgccacc accgcgctcg aatcgacacc tccggacaga aaggcgccca
     6061 gcggcacctc ggcgatcatg cgaatggtca ccgcctcgcg cagccgctcg atcagctctt
     6121 cggccagttc ggcctcgctg gcctgcggca gcggtgtgaa cggaatgtcc caatactcgc
     6181 gggaggccgg cacgccctga ccgcgttgca agcagagggt gtggccagcc ggcagcttct
     6241 gtacgctgga gagaatgcat ttcggatcag gcacatagcc cagagcgaag tactcctcga
     6301 tcgccgtcgg gtcgaggcgg cgcgacagcc gtggatcgca cagcagcacc ttcaactccg
     6361 aaccgaacag cagccagcca tcgatcagtg cgtagtagag cggcttgacg ccgagccggt
     6421 cacgggccag aaacagcgtc tgccgtggtc gatcccagag tgcgaaggcg aacatgccgc
     6481 gaaagtggtc gacacactgc tcgccccaga ctgcccagcc ggccaggatc acctcggtgt
     6541 cgctgtgggt gcggaactgc tggcccaagg cctgcaactt cttgcgcact tcgatgaagt
     6601 tgtagatctc gccgttgaac accaccaccc gttcgccgtc ggcgctgtgc atcggctgct
     6661 ggccggcgga gaggtcgatg atcgacagcc ggcggtgcgc cagaccgaca ccggcttcga
     6721 ggtgataacc accctcgtca ggaccgcgat ggaactgact ctggttcatc cgctccagca
     6781 gtgcccggtc aggctcacgc cgatcggtca ggtcgacgat cccggcaatt ccacacattt
     6841 ggctttccag tcaatcagtt gagtcagttc gagcatgcag tccggccgct gtcgagtcgg
     6901 tgaacgcaag ttgccgatag agcccgcggt agcgctccac catgcgggcg aggctcagct
     6961 cgcgctcgat gcgggcccgg ccggccgcac catggcgcgc acgcagcggc gcatcttcga
     7021 catagtcgag cacggcctgc accatgctct ccggttcatc cagcggcacc agttgcccgg
     7081 tcacccccgg ctccaccagc tcccggttgc cgccgaccgc ggtggccacc accggcaggc
     7141 cgcaggccat ggcttcgaga atggtgttgg agatgccctc ggccagtgac ggcagcacga
     7201 agaggtcgaa gctctgcatc cattcggcga tgttctgctg cgcacccgcc aggtagaggg
     7261 cgtgcgcgtg gccgctcttt tgcagtatag cctgcaccgc ttcgcgctgc gggccagcgc
     7321 cgacgatgac cagccgcgcc cgctggcgca gctccggccg ctgctgcagc agttggccaa
     7381 aggcgtgggc cagcgccaca tggttcttca cctcggcgag ccgccccacc gagccgatga
     7441 cgacgccctg ctcggggacg aaaccggccg gcagcagtgt gtcgcgccgc cgcggcagcg
     7501 gctggaactg ctcggcatcg acgccgttga tgatccgctc gatcttgccg gcggccacgc
     7561 cgatctcatc gtgcaggtag cgctcaccct cgaccgacag ggcgatgtag cggtcgatca
     7621 gcggcacgaa gagacgccgc agcatgcggt aacggcggtt tttgccatgc agatcggcca
     7681 tgtcccagcc atgttcgcca tgcagccgcg ccggcacccg cgccagccag ccgatggcct
     7741 gcgcctccag cgtcgccagg ttgcgggtgt gcagaacggc cggacgcaac tgccgcaaca
     7801 gccggccgag ttgccacagc atcgcccagt cctgccccgg ccgtttgtgc agcgcatggt
     7861 agctgacggc gggatggtgg atgcggcggc agaactgcgg gttgtagtcg gtcaggcaga
     7921 tcacggcatg acggtactca ctgcccagcc ggttgatcag gttgacgacg ccattctcca
     7981 gcccaccgat gtcgaagcga tgcacgacat gggcgatcag cggcggtgcg gtggcttcag
     8041 ccatattgcc gctggatcca gtcacggtag ctgccatcca tcacctcacg ccaccaggcc
     8101 tcatggtcga ggtaccaggt gacggtccgg gcgatgcccg actcgaagct ctccagcggc
     8161 ctgaactgca atgcctgctc gatcttgcgg gcatcgatcg catagcggcg gtcatggccg
     8221 gcgcggtcct gcacatgggt gatcagcgtg cggctggcca gaccccgcgc agccggcgca
     8281 tccggaaagc gcactgccag ctcggggttg gcggcaaagg cctgatcgag cagcgtgcag
     8341 agggtggtga ccacctccag gttgctcttt tcgttgttac cgccgatgtt gtaggtctca
     8401 ccgagctggc cgcgctcgat caccgcctcg atgccgcggc agtggtcggt gacatagagc
     8461 cagtcgcgaa tctgctggcc atcgccatag atcggcaatg gacggccgaa caggatgttg
     8521 atcaggcaga gcggaatcag cttctcggga aagtggtagg ggccatagtt gttggagcag
     8581 ttgctggtgg tggtgcgcaa cccgtaggtg tggtggtagg cgcgcaccag gtggtccgat
     8641 gccgccttgc tcgccgaata gggtgaattg ggggcgtagg gggtggtctc ggcaaagggc
     8701 ggatcggtcg gcgccagcgt gccatagacc tcgtcggttg agacatggtg aaagtggtgc
     8761 gcctccaccc tgttctgccc cagccagacg ctgcgggctg ccttcagcag gctgtgggtg
     8821 ccgacgacat tggtgtcgat gaaggcatcg ggtccagtga tcgaacggtc gacatggctc
     8881 tcggccgcga agtggaccag cgtgtcgatc cgctcttcgc gcagcagttg ctcgaccagc
     8941 tcggtgtcgc agatcgagcc cttgacgaag cgcaaccgcg ggtcatcggc gaccggttgc
     9001 agactgcgca ggttgccggc ataggtgagg gcatccagca ccagcagccg gtcgtcgcga
     9061 tggcccgcca gccagtggtg aacgaagttg gcgccaatga aaccggcgcc gccggtgacc
     9121 agcagccgtc gcggtcggtg attcatctct gcagctcctt cagcatggta cgcagttcga
     9181 cccgccagtg gtcgggggtg aagccgaatg ccgcccagct cgcctgcttg tcgagcacgc
     9241 tgtaggcagg gcgacgcgcc ggtgtcggat agtcggcggt ggtgatcggt ctgatctgca
     9301 gcggtcgctg cagcagtccc agtgccacgg cctcctccat gatggcgacg gcgaagtcat
     9361 accagctggc cacgccggca tcggtgtagt gatggatgcc gcgcagtgcc ggctggccgg
     9421 cagcggccca cacggcacgg gccagcgtgg cggcatgggt cggggtgccg atctgatcgg
     9481 cgaccacccc gaggctgtcg cgctccgcca tcagccgcag catggtcttg acgaagttgc
     9541 tgccatggct ggcgtagacc caggcggtgc ggatgatcag ccagtcgagc tggctggagg
     9601 ccagcagcag cgcttcggcg gcctgcttgc tggcgccata gacgccaagg ggtgcgggct
     9661 gatcctcagt gcggtagggt cgactctgcc ggccatcgaa gacgaagtcg gtcgagatct
     9721 gcatcagccg gacagcgctg gcgctgcaag ccgccgccag cgccgccacc gcctgggcat
     9781 tgatccgctg cgccagctcc ggctcctgtt cggcgcggtc gacggcggtg taggctgccg
     9841 cgttgatcag cagtgccggg cggtgctgct cgatcagcgc ggcaaggccc tcgggtcggg
     9901 ccagatcgag tgcggcacga tcgagcgcga ccagctcgac cgtggccggc gcgctgcgga
     9961 tcaacgcgtg accgagttgg ccagtggcac cggtcagcag caccttcatg ggtcgagctc
    10021 cgcgtcgacc agccactgtc cctgctgatc cttggccgac agcagcggcg gctgctcggg
    10081 cagcagcggc cagtcgatgg cgaggtcagg gtcatcccag cgcaggctgc gctcatgttg
    10141 cggggcgtag tagtcggtgc atttgtagac gaactcggcc acgtcgctca gcacatagaa
    10201 gccatgggca aagccgggcg gcacccagag catgcgcggc tgctccgccg acagcagcac
    10261 cccgacccag cggccgaagg tgggcgagct gcggcggatg tcgaccgcga catcgtagac
    10321 ctcgcccagc gtcacccgca ccagcttgcc ctgcggctgc tgcaactggt aatgcagacc
    10381 gcgcagcacc ccctgggccg aacggctgtg gttgtcctgc acgaagtcga gatcgagccc
    10441 ggcggcagcg aagtggcgac gctgccaggt ctccatgaag aaaccgcgcg catcgccgaa
    10501 gcgctgtggc tcgatcagca cgacctcggc gatctcggtg gggatgaact tcatggtgcg
    10561 ggctcctgct gtcggctcaa caggccgaga agatagctgc catagccgct cttcttcaat
    10621 ggctcggcca gccgggccag ctgtgcggca tcgatgaagc ccatccggta ggcgatctct
    10681 tcggggcagg cgatcttcag cccctgccgc tcctcgacca cccggatgaa gtgggccgca
    10741 tcgagcagcg agttgtgggt gccggtatcg agccaggcgg tgccacggct gaacagttcg
    10801 acggtgaggc ggttgcgctc caggtaggtc tgattgacgt cggtgatctc gagctcgccg
    10861 cgcggcgagg ggcgcaccgc cttggcgatc tccaccacct ggttgtcgta gaagtagagg
    10921 ccggtcaccg catagtgcga cttcggttgc tgcggcttct cttcgagcga gacgacgcgg
    10981 ccggcggtgt cgaactccac cacgccgtag cgctctgggt cctgcacgta gtagccgaag
    11041 acggtggcgc cctcgctgcg gctggcggca gtttgcagct tcttcgacaa cccctcgcca
    11101 taaaagatgt tgtcgccaag gatcaggcag accgggctgc ggccgatgaa ggactcgcca
    11161 atgatgaagg cctgcgccag gccatccggg ctcggctgca ccgcatagct gagattgatg
    11221 ccccagttgg caccatcgcc aagcagccgc tgaaaggccg cctgatcggc cggggtggtg
    11281 atgatcagaa tatcgcggat accggccagc atcagcgtgg ccagcggata gtagatcatc
    11341 ggcttgtcat agaccggcat cagctgcttg ctgacggtca gtgtcaacgg atgcagccgg
    11401 gtgccggagc caccggcgag gatgatccct ttcggggggt gggtgacggg gttgctcatc
    11461 gggctggttt ttccatctgt gattgctggt tcaaagtctc ggtcaagaac tgtgccacgg
    11521 cctgcagccg ctggcggtcg gcgacactgt cggtttcgct gtagggggtg aagatcacca
    11581 cccccaccgc ccgggtgtcg cccagaatca gccggttgta ggcggtcagc agtttgcccc
    11641 agtagggatc ggtggtctgg gtcggaccga tccggttcca cgaccagacc agcagctgct
    11701 gtggcccatc gaggcgtccc tcgcgcagcc gggtcaatcc cggcagcgcc tcggcctcga
    11761 tcagtcgatc gaccggttga tgccagagcg gatgcttctg cgggatcagc acgttctgcg
    11821 agttgatcag ttcggcatcc tgccgctgca gcggatagta ggctagatag acgccgaccc
    11881 actgaccctg ctgccggtac cagccgagct gctcctgcgc catgccctga tagtggggct
    11941 tccagtcggg cggtggcgtg ccatcgagcc gccgccagtc gccgagtcgc tcaggcaggc
    12001 ttggtgctgc ggcggtcggc accgtcgtgc tctgctcgct ccactgctcc agcagcggcc
    12061 agccgccgat cagcagcgcg gtgaccagca gcgtgctcca cgcactggcc gtggcgccaa
    12121 ccgtcgatct gctgcgaccc ggtgctggtg cgcggtgagc gggcggctcg ctccagacca
    12181 taccgatggc gaacaggatg aacatcacca gaccaaagaa gacccaacca tagatgtagt
    12241 gatcgacgcc cagcgccagc ttcatgtcac tgtagtgggc aatcatgacg atcatgaagg
    12301 cgcgcagccc gttggcgacg atcggcacga cgatgaacag cagcatgaac agcagtcgac
    12361 gaccggtcga gcggaagttg atgtagctga acagcgcgcc cagcgcgatc gaggcgatca
    12421 gatagcgcac gccgctgcag ccctcgacca ccgaccagtt gccactcggc agcgtgaaga
    12481 aggtgccctc ggcgaagatc ggaatgccgg tcagcttcac cgcattgatg gtgaagaagg
    12541 cggtgaactg catcagcggc ggaatgaacc cctcgccaaa gggcaccatg aagaagagat
    12601 aaccgagcgg aaagagcagc cgccagacca ccgcgctgcc gagcacggcc cacaccgcca
    12661 gtggcagcat cgccacccag gccgcctgct cgacgacggc cacatcgacc agccgcgcca
    12721 gaaaccagcc cagcccggtc ggcagcaaca gcagccaggg ccaacggctg gtgcctggcg
    12781 tcagcgccgc cagttcgccg cggcgcagcc agatcagata gagggtgatc ggcaggatca
    12841 aaaaggcgtg ggtgaaggtc tccgagcgcg cccagatctg caccatcgag ctgaaggtcg
    12901 gccagaaacc ggcaaacagc agcgtcagca gcagcgccag tcgcagcagt ggccagcgcc
    12961 aggcgaggat gggttgcaga gggtcagtgg ccatggagac tccttggggc gcgattttcg
    13021 atcacgctgt cgagcagatg gtagctgttc tgccaactgt agtggctcag catcgtctgg
    13081 cggcccagcg cccccaggct gtcgagccgc tccgaggtca gcagccggtc gatccgctcc
    13141 gccatcgcct cggcgccctc ggcgacgatc tcctgcatcc atggctgtgg cgtgatgccc
    13201 tcgagcgctg cggtcgagac cacgatcggt cggcccatcg ccatcgcctc cagcaccttg
    13261 ttctgaatgc cccgcgccac ccgcagcggc gcgaccgcca gccgcgcatg ttgcagatag
    13321 ggccgcacat cggccacgcg gccggtcacc accacatcgg gcgtgcgcgc cagcgccagc
    13381 acctggggcg ccggattgga accgacgatg tagaagcgca gtgccggatg gcgggccttg
    13441 agccggggga agatcgcctg cgcgaaccag ccgaccgcat cgatgttggc ccagtagtcc
    13501 atcgcaccgg tgaagaccag cacctgctcc tgcggagcat agggggatgg caggctggca
    13561 tcgggcgcga agtagtcgag atcgacgcca ttgggcacgc tcagcacccg ttcggcgctc
    13621 tcgggcgcga agccgcggaa cagcgtggcc tcgtgctcgg agacgaacag cgacgcctga
    13681 aactgccggg cgatgccgcg ctcgaaccgc agcagcgtgc gctgttcgcg ctgatagagc
    13741 cagcgcagcg gcgggcgatg gcgctcgctg tattgacgcc atttgtccga gtcgacatcg
    13801 acgaagtcga tcacccgatg cagaccgcga tgccgcgcag ccgtcaggta ctgcgccatc
    13861 ggcgacgaga acaggaaggc cttgccgatc ggttcgcgcc gcagcgtggc ctcgacccag
    13921 cggcgcaggg tcgcgctgcg gtagtggaaa ttggtcagcg gggcaccgcg caacagcccc
    13981 cgtgcggccg ccaccggcag cgtgccgcgg cgcagccgga tgaagcagct gtcggcgcag
    14041 agggcttcga gccgggcgcg gtgcggcaga tcggccgcgt catcgatgaa ggtgccgaga
    14101 aacagccgat agcgctcggc cagatggcgc agaaagtggt aggagcgcac cttgtcgccc
    14161 ttgtcgggcg gatagggaat gcggtgggcg agaaacagca gcggctccct ggtcatggct
    14221 caacccagat agcgcgagac ccaggggccg atccactgac tcagccacag tggcagccgc
    14281 tgccaggtgt tgatcagcag gcgatacttg gggttgagcg ggttgaggtc gggcagcgcc
    14341 tggcccttga ccaggtggta ttcgtaatag agcggctccg gcgtgaagcc ccagttcttc
    14401 ttgaagtcgt aggcgccggt gccgatcttg ctgcggccat agtcgaacag cctgatgcca
    14461 cgccctgccg cgtggcacat caatgcccag tacatgaagt cattggcctt gcaggcacgg
    14521 gccgcctcgg tgccgccgcc atagtagggc agcacctgat cgcgaaagta gaagctcatg
    14581 acgctggcca ccgtctcgcc ctgccgggtg atggtgagaa tctcgcactc atcgccaaac
    14641 acctcgcgca gcacccggaa gtactttcgg gagaagaccg gcgtgcccag gttgcgcacg
    14701 ctgctgctgt aggcatgaaa gaaacggtcg atgccggtgt cgatttcgct gtgcagtccg
    14761 gcgtcgatgc ccttgcgcac caccgcccgc tgcttgcgcg gaatcgcctt gaggttctgc
    14821 tcgtcgtcgg ccgagatctc cttgcgaaag gtgacataga gctccttgca gggccagtcg
    14881 ggcgcctgcc ggctcatgct gcgcagttcg aggtggtcga cccccagctc ggtcgcgagc
    14941 cgggtggcgg catcgagcag tgcatcgcga tggtggggcg cagtggcgat gggtccgcca
    15001 tagacacaga agggggtcga gaccagtgag tggccgaaca gccgactctt cacctctgcc
    15061 agcggcagca cagcctcgat ctgcccctgc tgctccagat agagaaagtg ggtccggtgg
    15121 ccgaaggcct gctccagcac ctgctgccag ccgctgagat gaaagaaggt gccctccggc
    15181 cgggcgcgca cgaaggcatc ccagcgggcg cggttcgaca catcgagcgg atggatggcg
    15241 tggtcggtgg tcaagcgtct ctcctcaaaa gcgggcgtcc cagacactgc aagccggcgt
    15301 tgtcacggca acggccgatc tattcggaca gaaagacgcg gtccattcga tcccagtgaa
    15361 aatcacccag cagccgggaa acccgtgact ccatccgctc gaggttgagg tagtgacgaa
    15421 agcggctcct gaagccggcg ccggccgggc gcggttgatc cggatcgatc tcccatggat
    15481 gaaaatagaa cagtcccgga gacttttcct gctggttgaa ccgcagcagc gcctggcggt
    15541 agagctggta gggcagcaga cggaaataac caccgccacc gatcggccag ttgcgcccgc
    15601 cgagccgcag cgtggtgatc gggatctcgg tcaggccgct ggagcagcga tagggaaagc
    15661 gcggagcatc gggcatgccg tagaggtcat gccgcaccgg atagacactg gagctgtaac
    15721 ggtgaccggc ttcggcgatc tcatcgtgcg cccagggggt agtgacatcg atcgagtagc
    15781 tgggggcgcg gtaaccatgg accgccatgc ctgagaggtc ctcgatcagc gccttggcat
    15841 ggcggatgtc gctgcggaac gcttcgcgtg actggctgtt gacgcgggca tgggcatagc
    15901 catggttggc cagctcatgc ccttcggcgg cgatgtcgcg caccagttgc ggcagccgtt
    15961 cggcgaccca gcccagcaga aagaaggtcg ccttgatgcc gcgctcggca aagagcgcca
    16021 tcacccgacg ggtgttctga tcgacccgca gtggatagct gtcccagcgg gccggatcga
    16081 tcagcccctc gaaggccgcc acctgaaaaa aatcctccac atccagcgtc atggcattgg
    16141 tctgccgcac tggcgccacc gattcgccct gcttcatagt ggcagatcac cgcgatcgag
    16201 ccatgccagc aggtcatcga caatccgctc ggcggcatgg ccatcccaca gctccggcgc
    16261 ccggccacgc ttgccaccgc tggcgaggat ctcctcgacc tgggccagga tggtggccgg
    16321 atcgcgtccc accagcgtgt tggtgccttc ggtgagggtg atcggccgtt cggtgttctc
    16381 gcgcagtgtc agacagggca cgccgagggc ggtggtctcc tcctggatgc cgcccgaatc
    16441 ggtcagtacc agacgggcat cgcgcatcag accgagcatc tccagatagc cgagcggctc
    16501 caccggcagc agcgtctcgc catcgagcaa cgactccagc cgctgctgga cgatctgggc
    16561 gcgggtgcgt ggatgcagcg gaaaaatcag cggcaaccgg cgaccgatct ggttcagcgt
    16621 ctccagcagc cgacgcaaca cctgcggatc atccacattg gccggacgat gcagagtgac
    16681 gacgccatgg ccggcgccgc gtgccgccac ctgccgctgc ggttgtgcca gcgccggaat
    16741 ctgcgccagc ggcagtgccg cccggctgtg atggtgcagg gtgtcgatca tcacattgcc
    16801 gacgaaacgg cagcggctgg attcgatgcc ttcgcgcagc aggttgtcga aggcactgcg
    16861 ttcggtcaga tagagccgtg ccgacagctg atcggtgatc agccggttga tctcttcggg
    16921 catcgagcgg tcaccgctgc gcaggccagc ctcgacatgc accaccggga tgccccgctt
    16981 gatcgccgtc agcgcgcagg ccaccgtgga gttgacatca cccaccacca ggatggcggc
    17041 gggccgcacc cgctccagca ccggctcgaa ctgcaacatg atctgggcgg tctgcacggc
    17101 atggctgccg gagccgacac caaggtcgat gtcgggctgc ggaatctgca actgctcgaa
    17161 aaactgccgc ttcatcgatt cgtcgtagtg ctgcccggtg tgcagcagca gggcaggacg
    17221 ttgcggcgca cgcgcgcgcc acgccgccat gatcggagcg atcttcatga aattggggcg
    17281 ggccccgacg acacagagca gtggcgcgct catcgtgggc agttcagagc gagcttttca
    17341 aggcgtggac gacacttcgc aacaggctga tcttcttgtt gaagttcttc tccagcacct
    17401 cgatcacata ttcgatgtcg tgaatgcgct tgtcgagttc gctcatcgac aacggcgccg
    17461 tgcgtggaag ctgcgattca ctgccgcttt cgaactcatc gtcatcggca tcgtcacgga
    17521 ccggcgagcg ggtcatcggc agtggtcgat tgctgccgtt ctcctgggcc agctcgcgct
    17581 cgaccactgc gaccgcctcg ctgctcagcg tcttcagctc ttcgagatag ccgtagagca
    17641 gcagccggtc acaaaaactg ttgatgcgcc gcggcacccc ctgggtgtgg cggtgaatct
    17701 catcgaaggc agcgtcggtg atctccggat tgtcgccctg ccagccgacc agcttcagcc
    17761 gatgctcgat gtaggcacgg gtctccagct gatccagcgg cgtcagatgg caggaggcga
    17821 tgatgcgctg gcgcaactgc tccatgttcg gcgactgcac gatgtggcgg aactcctcct
    17881 gacccagcag aaaactctgc agcagcgggc ggttctcgat ctggaagttg gagagcatgc
    17941 gcagctcctc gacggtcgcc ggcggcaggt tctgcgcctc gtcgaccacc agcagcaccc
    18001 gtcgcccctc acggtcggcc tggcgcagaa aggcctcgaa ctgatgcagc agcgccgcct
    18061 tggtcaaccc ttcggtcggc aggttgaagg cggtcgagat catcttcagc atctcgtcgg
    18121 cctgcaggtt ggaggtgacc agctgggccg cgatcacatc gtcatagccg aggtcggcaa
    18181 acaggttgcg gatcagcgtg gtcttgccgg tgccgacacc accagtaatg acgacaaagc
    18241 cctcaccctg actgagacca tagcgcaggt aggacatcgc acgcttgtgc gccttgctgc
    18301 catagaagaa gcgcgggtcc gggctgacct gaaacggctt ggcgctgaat tgatagaact
    18361 cttcgtacat ggtgacggcc tcaaaaacgc atgttgccgc tcaacgtcag gcgattttcc
    18421 cggtaatcgt tgagcaggct gccctgggca tcgttgctga tgcggcggta ggtcgccgac
    18481 agtgatgcat cccgtgtgac tgtctgggtc aggcccagat gaaactcgtc gtagtcgagc
    18541 ttttcgccgg tcatgaagtc gcgggtcaac cagctcacac cggtcgtcag actggtccgg
    18601 ccactgagac tgagggtcca gtcgaaattg gcgccgcgat cctgggattc gttggatgag
    18661 gtcagatagg tgcgatcgac ctgatagccg ttgagactga aggcggaacg accccgctgg
    18721 taaccccagg tcgcggtgta gcgcttttca agaaattgat catcggcgat cacacccacc
    18781 ggcagaaaga gtccgatgag ttgctggttg atccccagct gcactggctg gttcggattg
    18841 acgaacacgc actgtgccgg attgaaggtg gtgccgctgg gacagatcag ggtgccgatc
    18901 tgctgattct gcagcaggtt ctgccgcgta ttggtcacct cttcctcata cttcagcgag
    18961 aatgacgagc gacgcagccg gtgggtcagc gagaaatcgt aggccggacc ataaaagtcc
    19021 tcattgtagc caccctccag atgccagcgc cggctgggat cccatgaagc acggccaccg
    19081 aacgaaccct tgttctctgt ctcactcagg gtgtcatcgt cgtactcacg cacctggtag
    19141 aacaggtcga actggctctt gcgcgagggg acccagccga tggtcgcccg gtagtagtgg
    19201 ttgttgggct ggcgattgtc ggcgaccagt gtgctgttgg gcgtgtagct gtattcgctg
    19261 taacctgcgg tgccggagaa gcgcagccgc ttgccggccc agacaccgag ggtggcatcg
    19321 ccattctgct ggccaccatc ctgctggctg ttcgcgtagt cgatgttgtt gtcgaggtag
    19381 ttggcgctcc agaacagcgg cattcccgcc gaaccgctgg tcagattgaa ggtcagcgtg
    19441 ctgccctgac tctggttcag attgccactg ccaccggagt agtcgacata atcgacgcca
    19501 taacccacct catagcttcc ccagccaccc agccgctggg tccagcgcgg ttggaaatag
    19561 ccgcgccagg cggtggtgat gttctgctca ttgcccagca gatcggcatt caccgaccgg
    19621 gtcgggtcga tcagctcctg cgatgccagc acgccggcat cgagaaagag atgctgtttg
    19681 atcgcctccc actgacccgc tccgttaagg ctatggtagg tgttgtcgag gccgctctgc
    19741 tgcaggtggg tcagctgcgt caacctgtag ccgaggttca gtttggagcg tccgctgttg
    19801 cgctccagat cgaaaccggg ccgggcgatc aggatggtgt ccgagtcgcg gtcgttcttt
    19861 tcgagaaaga tgttgtcggt gtaggtcagg tcgacattga ccgagggtgc cgcgcgccag
    19921 ctcacggcat ggcagggcag tccgatcacc aacaacagcg ctccacccag tcgataccat
    19981 ccacggtgca tgtcgttact gtccatagcc atagtaatag tagccatagc catcgctggc
    20041 atggtggctc ttgttcagaa tcagaccgac atcgacactg gtacccatca gtgacaggga
    20101 ctttttcacg tcagcctgcg gggtgagccc ttcggccacc accagcacca cctgccctgc
    20161 cagttgtgcc agcacgctgg cttcggtggt gctcagcagt ggcggggagt cgatgacgat
    20221 gatccgatcg gcatagcggg tggccagttc atggaccaac tgctccattc tcttgctcgc
    20281 cagcagctcg gtggtcagat ggtgacgttt gccggcctgc atcagggtga actggggaat
    20341 gttggtcttg tacatcagtt cgccgggcgt gtcgctgtcg ccggtcaggt aatcgaccag
    20401 gccagcaccc tcttcgaccc ccagtttctt ggcgacggtc gatttgagca cgtcggcgtc
    20461 gatcagcaac acccgatggt tgcgctcctc ggcaatgctg agcgccagat tgatggcatt
    20521 gaaggttttg ccctcgcccg gacgcgcact ggtcaccatg atgatgttgc ggtgattgcg
    20581 ctgcttgtcc ggcaatccgg cgaccttctt cagcagcggc cgcttgatgt ggcggtactc
    20641 ctctttcagc cgggtccgct cgtcatcagg gatgatcaga ccgccttcac gcagatggtc
    20701 gaaatcgatc tcgacatagc gactgctctt gacttcgcag tagggccgcg actgcgcttc
    20761 tggatcgatc tccggcatgg cctgcgcggt gacggaatgc ggcggttcat caggcaatct
    20821 gaccacggcg ccgctgcccg gacggcgccg ggcttcgctc tgctcgccgc ggcgctcctg
    20881 gagtttcttg aaggcttttt cgatggtgtc cataaggtga ttcagtgtgg attgcagcca
    20941 acgaacgatg cagtgatcgg ggttgttccg tcagcccctg atgaaagcat agtaggaagc
    21001 aagcgcggcg tagaccgcca ccagcagcaa cgcgcctgcg acaaacagga tgctctccag
    21061 ccggtgccgc ttgcgcaggg tagagctggc cacgaaggag acggtaccaa gcacctgcag
    21121 gttggtcatc tgcgccagca gcagtgtgct ggtgaaggtg gggcgcaaca ccgcgaaaag
    21181 cgtcgccacg ccaagaccaa gcagcaatcc gccgcccagt gtggccgaca tcagcaacag
    21241 tcgattgggc ccggccggtg ccgtcggcac gcggggcgga tcgatgaccc ggaactggat
    21301 gtcgttggcg tcctcttcga ccctttgcga gatcagcgcc gactcgcgcc gaccgaggaa
    21361 ctcttcatac ttctgcttgg cgatgccgta gtcgcggttc aggttggcaa gatcggcctc
    21421 gatctgcggc acagtgtcga cccggttctg cagatcgcgc actttctgtt cataggactg
    21481 gacccgggca ttgagcgatg ccagttcggc ctcggcctgc gagaaggcga gcttgagctg
    21541 ctgtttgagc ggatcggggg tactgctgaa ttcgctctgc ggctgctggg cctgggtctg
    21601 cagatccttc tggcgcaact cttcgaggtt cttcaacgta ttgcgtattt ccaccacgct
    21661 gggatggttt tcggtgtatt tgagcagcag gctgtcgagc tgctcattca gccgcgcaat
    21721 gcgcccatcg tattgggtgc tgccggagcc tgcgccgggt gtcggcaaca gcagttcatc
    21781 atcgccaccg gacgaactcg cgctcgcctg cacctgttcg cgcagggcgt cgcggcgctg
    21841 ctgcatctcc ttcacctgca ggcgcgccgc ctccagctgc tccgttgcac tcttcagctc
    21901 cccgaagtag ccgccactgc cggacatgaa ggcgacattc ttctgcttga actccttcag
    21961 ccgatcctcg gactcacgca gacgggcttc atactcctcg atctgcttgt cgaggaagcg
    22021 ctgcgcactg gcggcggcac catcgccctt gccctcgccg atggtgttct cgacgaaggt
    22081 ggtcaccacc gcctgcacca cccgcttggc gcgctccggg tcggagtcgg gatagttgat
    22141 cgagtagaga ttctcctcgc tgtcccgctt catgtgaatg cggttgccga ccgaggcgat
    22201 cagggcttcg aactcctcat cggccccggc cgagagatcg agatcggcca tgcggatgat
    22261 tttttcgaga ttgggacggc tcaacagcgt gcgctccatc agccgcacct gctgattgac
    22321 atccatctcg atggtcagac cgcgcagcag cggcttgagc agactcttgg tgtcgacata
    22381 gacccgggcc gaggcctcat actgatcggg aagccgctga accaccaccc agccaatcgc
    22441 gcagaccagc caggtgctgc ccaatatcca ccacttgcgg cgcagaatgt cgcgcagata
    22501 ggagaggacc agttcgatct gttcgttcac tgccaggact cgttggaaag tggaaccacg
    22561 ggggcgccct ggccgccggc cacagaccgg ggttgggcag attcaggctg gtcttagaac
    22621 caggcctccg gaatgatcag gatgtcaccg ggccgcatgt cgacattggc ggagatgtcg
    22681 ccaccgttga ccagatcatc caggcgcacc cggtagacct cctgctggcc attgacgatc
    22741 cgcgccaact gcacccggtt ggcatcggcg aagtcggtca cgccacccac cgcgatcatc
    22801 acatcaagca gcgtcatgtg ctgtttgtag ggcagcgatt gtggtcgggc cgcctgcccg
    22861 atgatgcgga tctgctcgga ataggggccg ttgaagccac cgaccatgat ggtcacgatc
    22921 gggtctttta cgtacttggc cagcgccacc tcgacctgtc gcgccatctg cgtcggcgtc
    22981 ttgccggtca ctggcagatc ttccatcagt ggcgtgctga tcttgccatc gggccggacg
    23041 gtgaccgaca tcgacacttc ggggttgttc cagacgaaga tctgcagcga gtcaccagga
    23101 ccgatcaggt agtcgtagtc ggcccggtcg gcattgaagg agggatgaag tgtggcgggt
    23161 ggcagggttt gctgaccgct gttcaatgtg ccacagcccg caagcatggt ggcacagatc
    23221 aaacccgcaa cgaaatggac tctgttattc atgcgctccc ccgacactcc atgattgaat
    23281 tcacccagcc agcatcatgg ccggcatcaa gtcctgaaag agtagcgcat ggcgcagaaa
    23341 aagcccaacg ggtaccggca atcccggcca tgaccgcaca ctgcaacact tgtcgacgcg
    23401 gtcatgggtg atcggcgtca cgcctgccct cgagggttac agcatgatgg caggttgtgt
    23461 ggcggggcgg tgtagcgatt tctggggttg tggatcgagg tcgccaagcc gtcggcagaa
    23521 gggttcactg tagtagaaac ggcgcagctg gggtgactcg gtcatgatcc tctgtcgtga
    23581 cctctccacg tccaccaggt agggatggcg ggtgccattc agttcgcaag gttcacccac
    23641 ctgatcggtg tggatcatgc actgctgcag tcttctggcc agtgcgcggg tggtcagaaa
    23701 ataccactgc ccgatctcct catcctggct gacgctgaag gccgcacgca ccatgcccca
    23761 gaagatgcct ggctcgtaga cggcaccctt ggcagcgcgc ttcatgtccc agaagttgcc
    23821 gctgtggctg cgcaaaacgc agagccgcga gagttcgacg atgctctgcc ccggcagctg
    23881 tgtccaccac cccggtgcga cctggcaatg tgaatgcagc ggcagttggc cactgctgct
    23941 cctgaccagc cgcatcgcgg ccacatactc gccactcagc cggcagcgca cgatgaactt
    24001 gaacgagaaa gggtcccagc gatcatgctc gagcccctcc gggaattgct gccgatcctc
    24061 aaagccccgc tcctcgcaat agacgcggta acgcacggca aaatggagat cttcgctgtg
    24121 cacgccgttg gccaggaaca cttggtaatg gtcatcgaac agcatgactc tctccaattc
    24181 aacggatttt gaagatcgaa caaccatcct gtgcgccgga tcacagtgga tgccggctga
    24241 tgagcagacc ccggactaga ctcaaaccca caagcggagg cttttcaacc atcccatcgg
    24301 cacagtgatt ccttcaccat gatcgttcaa gcacgccctc ttcatcttcc tgcgcaggcg
    24361 actgctccat cttttcaaag ttccgcggcg caagcaacag acttgatcaa tttctctaca
    24421 ccacctgccg tggccgagat ggtctgcacg ctggcaccgg agcatccggc ccggctgttc
    24481 tggagccact tcaccccgct ctacgccgac agcgaggcgt tgcgcgatca ggtgtttcgc
    24541 atccgtcacg aagtcttttg cgaggagttc ggcttcgaac cactgagcgc ggatcagcgg
    24601 gaacgcgatg agttcgatcc gatcgcccac cattttctgc tcggacacaa ccagtcgcag
    24661 cagtttgccg gctgcgtgcg tctgatcgtc ccccgtccgg aagccaatca gagcatgttg
    24721 ccgatcgccc gtttctgctg gcctcatctc gatccctatg tgcagatgct gctgcagagc
    24781 cgggcccgtt gcggagagat ctcccgtctg gcggtcagag cccgctaccg acgtcgccag
    24841 agtgatccgc acacgccgga gggtggtgca ctccaggcag aggcggcaga gcgatcggcc
    24901 gatcggcgac tctttccctt tgtctccttc ggcatctatc tctgcatcgg cgccgcgctg
    24961 gagctgctgc atcagcgcgg ccaactggat gccgcgctga tcatgatgga gcccaaactt
    25021 tacagaaatt tgaagcagca gggcttcaac tttcagccag cgggaccggt gatcgattac
    25081 catggccgcc gggcgccctt cgtgatcacc aagcagaccc tggcacccag gctgtcaagc
    25141 gaaatgcagc tgctgaaaac gtcgatcgcc gtggcgctgg cgccggtcat ccagccgtaa
    25201 ttccaccctt cggccccgca tgtgaacatc aacctcacca ggaaaattct tcagcaccgc
    25261 accttttcca tcgtctcgat gctgctgctg atcgtgtttg gcagctacgg catccgcttt
    25321 ctcagtctca cggtcgacta ccgcgaatac ttttccgcca ccgacccgca actggccgcc
    25381 tacaacagga ttcaggccga ctattcgaac aatgacaaca tcctgattgc gattcgggcc
    25441 aggcagggcg atctgttcaa ccggccactg ctggcgctga tcgaagcact caccaccgcc
    25501 ggctggagtc tgccctacag tcaccgcgtc gactcaatca ccaacttcca gtacagccgg
    25561 gccgaaggcg atgagctggt ggtggagccg ctcttcgcac acctcgaccg gatgaatgag
    25621 gccaccattg cccagcggcg gcaggtcgcg ctgcaggagc cgcggctgcg tggcctgctg
    25681 ctggcaaccg atgcgcaggc gaccgcggtc aacatctcct tcacgctgcc acaccaggat
    25741 gacctggccg agaccgccgc ggcggtgggt gcaacccgcg cactggtcga gcgcttcgcg
    25801 cgccagcatc ccgaggtcga gtttcatctc ggcgggcagc tgatcaccaa tcaggccttt
    25861 ccggaggcga cctcgcaaga caccacccgg ctgtttccgc tggtgctgct ggtgatgaca
    25921 ctgatgatcg ccctggccat tccacacctg tggtcggtgc tgctggtggt gctgctgatg
    25981 ctcttttcgg tgacggtcgc gctcggcacc gtcggctggt tcggggtcag attgacgcct
    26041 gacagcatcg ccggctcggc gatgatcatg ccgatcgcca ttgccaatgc ggtccacctg
    26101 ctgctggcct attttcaccg actgcacaag ggcgtgcagc cgctcgaggc gatggcgcag
    26161 agcatcaccg ccaacttcat gccgatgttc ctcaccgcct tcaccacggt ggtcggtttt
    26221 ctggccgtca acttcagcga ctcgccaccc tatcggctga tgagcaacat cgtcgccatc
    26281 ggtcagaccg tgaccttcat cctcaccttc ctgctgctgg cgccggtcgc caccctcatg
    26341 caacgccgcg tggagatccg cagcgtgcag gatcggctcg acccgctgat gtgctggctg
    26401 agccgctgta ccacccggca ccagaaggca ctgctgctgt tcatggggat ttcgacagtg
    26461 ctggccattc cattgacgat ggccaaccgg gtcagtgaca acccgctcga ctggttctcc
    26521 gaacaggtgc cattccgcca ggatgtcgac tggatcaacc ggcacctctc cggtgtcacc
    26581 ttcatcgact acccgctcgc cgcggggggt gacatggaga tcaccgagcc ggcgtttctg
    26641 cagaaactgg atgccttcac cagttggctg cgcagccagc cacgggtgcg gcacgtggcc
    26701 agcatcagcg acaccttcaa gcagctgaat caggagatga accagggcga ggccgtccac
    26761 taccgcatcc cggagagccg cgagctggcc agccaatatc tgctgctgta tgagatgtcg
    26821 ttgccattcg ggctcgatct gaccaaccag ctcaacttca acaagtcgag cacccgggtc
    26881 agcgtggcgc tcgacaagct ctccaccgtc gagaccatcg ccttcgacca gcaggtgcag
    26941 cagtggatgc aggccaacct gccgccgcag atgcgctctc aaggctcggg gctgtcggtg
    27001 atgctggcgc acatgaccga ccgcaacacc cgctacatgc tctatggtgc cgtcgtcgcc
    27061 aagctgttga tggggctgac gctgctgctg gccttcaggt catggaagat cagccttgtc
    27121 ggcctggtgc ccaacatggt gccggccttc tatgcctttg cgatctggtg gttctgtgtg
    27181 gccgaggtgg ggctggcgct gtcgatggtg ctgtcgatga ccttcggcat catcgtcgac
    27241 aacaccatcc atctgatcga caaatatgac caggcacgcc ggacgcaggg tcatgcccat
    27301 gccgaggcaa ccctctacag ctatcgcgaa gccggtcccg gggtggtggt gtcgagcctg
    27361 tcgctctgct gtggctttgc ggtgctgatg gtctccgact tcacccagaa tgcccaactc
    27421 gggctgatga cgacgctgat catgctgctg gcactcggca ccaacctgct gtttctgcca
    27481 ccgctgctgg cctgtttcac gccggcacgc tggcgacatc tgcaaagcgc tcagcaagca
    27541 tcggggatgc aaggtcagga gtcggattcg ccgagcggcg cgtaacggcc cggcgcgatg
    27601 atcccttgcg gatcgagcgc ctgtttgatc ttcagcgcca tccgccagtg cggggtgtcg
    27661 gcatcgatca acgccgacat cgcatcgaca tgcagccgat agggaaagaa accctgctga
    27721 cgtccagccg ccagcagggc gtcgtagcag gcgcgggcgc gggcggtcgc caccgcatcg
    27781 ctgcggtcga acaggatcgg caccgtgctg tcgaagcagc gcggcgagat cgacgtaaag
    27841 gtgatcagcg gctcgatgcc atgttcgggg cagatgcgtt cgaccatctc ccgatagcgc
    27901 gccaccgcct ccggccgcat cggcaccagc ggcgcgtacc agatcaagcc acagccatcg
    27961 cgggccgggt ggtagtcacc cgccggcagc ggctgccggc tgcgccagta ggccagtggc
    28021 agggcgatct cgctcggttc gccggccacc acccgcaggc tgcggtcgag cgtctcgacc
    28081 agaccggcca gatgacgccc cggcgcaccg ggcagccgct gggtccacgg cagcagccgg
    28141 tggatggtcg agggggccag gaacagtgtc cggctcgcca cccccttcaa ctggcgggca
    28201 atcaccttgc gggcggcccg cagcagcggc ttctcgccgt aaagcgcccc gaaacccatc
    28261 caggccgaca cccggtttcg ccgcgccagt tgttgcacca atgcatcgga catgatgccg
    28321 tcgggcgcaa tttgttcgtg cggatagggc tccatcatcg acagcagccg ccggctgttc
    28381 atcaggttga cgccgccgag cagacccggc agccggtgca gcagctgctg gatcgcctgc
    28441 accgctgccg ccagcttctg gtcgtcatcg agccagaagt agagcgccaa catcgactgc
    28501 ggttcgggca ccagttgaaa ggtcatccgg gtgacgatgc cgaggttgct ctggttgaag
    28561 agtccatcga ggtggggacc gataccccat ttgaagcagc cctcgatctc gtcatggccg
    28621 agcgtggcca gcgccccccg gtagagggtg ccatcgggca gcaccgcttc gagcgactgg
    28681 atcgcggcac agtggtcggc caccggggtg atgccatagc cgcgctccag cgcgtttccg
    28741 atcagactgc aatcgggccc ggcgccggtc accggcacca gaaagcgccg tggctgctgt
    28801 tgcagatggc gccacagatc gccctgggtc acccccggtt cgacggtcag cagcgccaga
    28861 tcggggtcga gatgcagaat ccgcttcatg cgcgacagat cgaccagcag gcagccgtcg
    28921 gtcaccggca gcgcactgcc gtagccccag ttgcgtccgc tgctgatcgg atagagcggc
    28981 aggccatggt cgccggccag ccgcaccacc gcctgcacct gcccggtcga agccggtcgc
    29041 accactccca gcagttggcg cctgctgccc agagtgtcgg ccagatagcc gtggatctgt
    29101 tcgtcgagca gcagatgttc cgcacccacg acggcgacca gttgcgcctg caaatcggcg
    29161 ctgagccgag ggtgttgcat cgtcgaaccc tgtttttctg agccgcgtcg agaaaaatcc
    29221 ctacccgggc agatgcagcg tgcccgacgt caccgaaacc acgctgccgc cgacccgcag
    29281 cttcagcccc ttgccgggct ttttgtccat ctcggcatgg agcaggctct tgcgggtggc
    29341 gtcgaagcca cgctcgacgg taaaggtgta ggtgccttgg cgcgtgctgg gctgttcggc
    29401 caggtaggcg gcaaaggcgg gcagtgccgc cccgaccggc gggtcgtcgt gggggccgac
    29461 ctgcggtccc accagccggg catggaagtt ggcctcgctg ctttcggtct ggcggcagaa
    29521 cagcaggatc tgttgggcca gcatcgaggg ggcgagcgat tcggcccagg cgccgtggtt
    29581 gaagcgggcc tgctgcagtg catccagtga gcgcagcggc acgatcagat agggatggtc
    29641 acaggagacc agcagcggcc ggaacttgtc caagccgatg gcgcggcgct cgagcgagag
    29701 gatcgccgcc agctcttcat cggtcggaac gaagcggtcg accaccggat gcaccgtatg
    29761 ggtgaactgc acgaagttgg gctggctctg actgacggtg aggtgcacct ccaccggacc
    29821 gaagttgtgc agcagatcga gccgggtgtc gccctcctgc aacgggatgg cgccgatgga
    29881 gcccagcaca tggccagcgg ccagcaccgt gtggccggcg aaatcgacct cacggtccgg
    29941 tgtgaaaatg cgcacccgcc gctgctgggg ggtgtcggtc gagagcacga acagcgtttc
    30001 cgacaggttc atctcactgg cgatctgctg catctgctca ggttccagac catccgcaac
    30061 cggaaagacc gccacctggg cgccgccaaa ggggtggttg gtaaagacat cggtggtgtg
    30121 aaaggccagc tccatcgatt cgctcctcaa tctcatgttg atgccccgtc agccgggcag
    30181 tggcaatggc gatgtctggg tgccatcgcc tcgtcgctca tgttaaatga tacgttgcgc
    30241 gaacgttgca cggctgatgc agatcaacca cttgctgtga ggtcacacct cgaagcggta
    30301 gctgtcgcca tcgcgcacga tgcgcccggc ggtcggcgtg gcgaagtggg tgcccagcac
    30361 cagggtggcg ccatcgctga agcggttgag aaattcgtgg cgggtcgcgc gcgcctgatc
    30421 ggggtcgaca tcggcgatgt cacgccactg cggtcgtgcc acctgcagcg gatggtgcag
    30481 catgtcgccg gtgatgaccg cccgtgcacc ctgtgacgtg accatgacac tgacatggcc
    30541 cggcgtgtga cccggggtgg gaatcaggca gacctcgtcg ttgatgcggt gattggcatc
    30601 gaccagatcg accaggccgg cagcgaagat cggcaggatg gagtcggcac gggcctcctt
    30661 gccgaaggga tcctcttcgt tttgccagta gccccactcc gcctcgccga tcagatagcg
    30721 ggcattggga aaggtgggca accactgcgt tcctgacttc atggtgttcc agccgacatg
    30781 gtcgacatgc aggtgggtgc agagcacccg gtcgatcgac tcgcgcggcg cgccgaggct
    30841 ggccagctct tcgagatatc gcccctcgcg catgttccag tccggcaatc cacgcggttt
    30901 gtggttgccg atgcaggtgt cgaccagaat gcgggcaccg cacgactcga tcagaaaggt
    30961 gtggatgctg agcgcaaagg tgccgtcatc gttgagaaag tgcggcttga gccagtgcat
    31021 cagtggcgcc agattctcca gcgtggcatc gggcaccagc gcggtggccg gaatgcggct
    31081 gaccagatcg accacccggg tgatggttgc cgcgccgatt ttccagactt ccatatagag
    31141 gctcctgttg ttgtcatggc tgtcatcgtg cggcgaactg cccggcccgc acccgtatgg
    31201 aagcagccgc gccggcaaaa cgcaatcgat gcgctgtttc gagcctggca tggttttggg
    31261 taggatgcag cagacggaac ctggaattgc tgagttccgg acctcgacaa taaccacaac
    31321 aaggagcatt cagccatgac tcgactcgca gacaagttcg cactggtgac cggcggggcc
    31381 tcgggcctgg ggcgggccca atccctgacc atggcgcgcg aaggcgccgc cgttgccgtc
    31441 accgacctca acatcgccgg cgccgaggcg gtcgcggccg agattcgtgc gcagggtggc
    31501 cgcgccattg ccctgccgca cgatgtggcc gacgagtcga gctggcagga ggtgatggga
    31561 cagacgctga atcagttcgg ccggctcagc atcctggtca acaacgccgg cattgccgcc
    31621 agcgtcgatg ccgaagaagg cagcctggcc gactggcgcc atctgatgag catcaacctc
    31681 gacggggtct ttctcggcgt caagcatggc atccgtgcgc tgaagcagag cgggggcggc
    31741 tcgatcatca atatctcctc gatcatgggg ctgatcggct cacccggcag tggcgcctac
    31801 aatgccagca agggcggggt gcggctgctg acaaaatcgg cggcgctcta ctgtgccaag
    31861 gcgggatatg gcatccgcgt caattcggtc catccgggct tcatcgacac gccgatgatc
    31921 cagcgcatca tgcaggggcc gcaggccgag gccgcccggc agaatctgat tgcactgcac
    31981 ccgatcggcc ggctcggggt gccgcaggac atcgccaatg gcgtgctctt cctcgcctcg
    32041 gatgagtcgg ccttcgtcac cggctccgaa ctggtgatcg atggcggcta caccgcccag
    32101 taagattgcc ctccatcacc acgcggcggc agcgggcgac ccaatcagta cccgctgccg
    32161 ccggttggat ggcgtgacga tcagcccaga tcgaaccgat cggcattcat caccttgacc
    32221 caggcggcca cgaagtcgcg gacgaacttc ccggccgcgt cgtcgctggc gtagacctcg
    32281 gcaatcgcgc gcagctcggc gttcgagccg aacaccagat ccgcccgggt gccggtccac
    32341 ttgacggcgc cggtggctcg gtcatgcgcc tcgtagagcc ccgcgccggc cgccttccac
    32401 tccacgccca tgtcgagcag gttgacgaag aagtcgttgg tcagcacgcc ggggcgggtg
    32461 gtgagcaggc cgtgtctgga gccgccgtgc acggcgccga gtgcgcgcag gccgccgacc
    32521 agcacggtca tctccggcgc ggtgagcgtg agtaactgcg cgcggtcgag cagcagttcc
    32581 tcggccgggc ttgtgacgcc tgctttcatg taattgcgga agccgtccgc ggcaggttcc
    32641 agaacggcaa acgactcgac gtcggtctgc tcagccgtcg cgtcgccgcg accgggcgcg
    32701 aacggcaccg tgaggtcgaa cccggccgcc ttggccgcct gctcgatgcc gacgttgcca
    32761 ccgagcacga tcacgtccgc cacgctggcg ccggtcgctg cggcaatacc ttcgagcacg
    32821 cccaaaacgc gggccaggcg cgccggctcg ttggcttccc actccttctg cggcgccagg
    32881 cgaatgcgcg caccgttggc cccgccgcgc ttgtccgaac cgcggaaggt gcgcgcgctg
    32941 tcccaggcgg tggcgaccag ttcactcacg ccaaggccgc tcgcggcgat ccgcgccttc
    33001 accgcctcta tgtcgtagtc gcgtcgtccg gccggcaccg ggtcctgcca gatcagcacc
    33061 tcgctcggca cctcggggcc cagatagcgc tcgagcggcc ccatgtcgcg gtgcgtcagc
    33121 ttgaaccagg cgcgggcgaa ggcgtcggcg aactggtccg ggttctgcca gaaacggcgc
    33181 gcgattggct cgtagatcgg atcgaagcgc agggacagat ccgccgtggt catcatcggc
    33241 gggtgcttct tggacggatc gtgcgcgtcc gggatcaggt gctcgggttt gcagtttttc
    33301 ggcgtccact ggtgcgcacc ggccgggctc ttggtcagtt cccactcgta gccgaacagc
    33361 atgtcgaagt agccgttgtc ccagcgcgtc gggttcggct tccaggcgcc ctcgatgccg
    33421 ctggtggtgg tgtcggagcc cttgccggtg ccgagcgcgt tccgccagcc aaggcccatt
    33481 tcttcgatcg ccgcgccttc gggttcggcg ccgacgagct tcggatcgcc ggcgccgtgc
    33541 gccttgccga aagtgtggcc gccggccacc agcgccacgg tctcctcgtc gttcatcgcc
    33601 atgcgggcga aggtctcgcg cacgtcgcgg ccggaggcca ccgggtcggg gttgccgtcc
    33661 gggccttccg gattcacgta gatcagaccc atctgcaccg ccgcgagcgg gttttccagc
    33721 tcgcgattgc ccgaatagcg gctgttcggt ttgtccgagg tggccagcca ctcggcctcg
    33781 gcgccccagt agacgtcctc ctcgggttcc cagatgtcct cgcggccgcc gccgaaaccg
    33841 aaggtcttga agcccatcga ctccagcgcc acgttgccgg ccaggatcat caggtcggcc
    33901 caggagatgc gcttgccgta tttgcgcttg atcggccaca gcagccggcg cgccttgtcg
    33961 aggttgccgt tgtcgggcca gctgttgagc ggcgcgaagc gctggttacc ggtgccggcg
    34021 ccgccgcggc cgtcggcgat gcggtaggtg ccggcgctgt gccaggccat gcggatgaac
    34081 agcccgccgt agtggcccca gtcggccggc caccagtcct gcgagtcggt catcagggcc
    34141 ttcagatcgg ccttcagcgc ggcgtaatcg agctggttga actcggcggc gtagtcgaag
    34201 ccggcgtcca tcgggtcgga cttggccgag tgctggctga gaatcttcag gttcag
//
LOCUS       DAORCQ010000014        33712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_672_length_33712_cov_5.847045, whole genome shotgun sequence.
ACCESSION   DAORCQ010000014 DAORCQ010000000
VERSION     DAORCQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 33712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33712
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_33712_cov_5.847045"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKI23_02495"
     CDS             <1..451
                     /locus_tag="PKI23_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNN35841.1"
                     /translation="RENKEIKAPQPKPVEAPQAPQPLKMEGAAGEGSSPFAAGTVSSD
                     YIGGKVGGGGGGMQFAFFSSALQRHIQQELARNRALKLGDYRVLVNVWIDAQGVMRRV
                     ELDESTGNPDTDRAIREALNQLPPLRTAVPENLPQPIRLRITNRMAG"
     gene            502..2169
                     /locus_tag="PKI23_02500"
     CDS             502..2169
                     /locus_tag="PKI23_02500"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNN35842.1"
                     /translation="MTFKTLRCGVLLASALLSAPVWAEDSRASLEALRQTTLNLINAL
                     VDSGAITRQKADELIKKAEQDAAATVAQTPKRADNTVRVQYVPEVVKSEMREQIKQEV
                     LEQAKREHWATPNAIPDWLDRIKWEGDLRLREQLDRFDENNATPFDYATAAGTNAPPP
                     LPNDQTRAAEFAKVNGQGIPTANTTEDRDRLRLRARLGMLARLSDEWSGGVRLATGSN
                     SDRVSTNQTLGQDFNKYTLLLDRAYLKFDPTDWLSLTGGRIPNPWFSTDLVWDEDLNF
                     EGLAANFRPVLSSSRVKPFVTLGAFPLQEENPPSSSGRWLRGVQGGTQWDFSPDMRLK
                     VGLAWYDYQDLAGEVEPASALDSTGNPITANYGQYEYGNGLRQKGNSLFVTNALGDTG
                     STPYWGLAAEFRPVNLTASLELAQFAPVHVVLSGDWVKNTAFDRAQILSRTQGRINLT
                     DGSDQGYQYRLTVGTAEVRNRRDWQLSVAYRHLGSDAVLDAFTDSDFGLGGTNLKGYL
                     LGAQYGLDRNTVLNLRWLSADSIDSFSFIADHKFAVDLFQADVSVKF"
     gene            2172..2840
                     /locus_tag="PKI23_02505"
     CDS             2172..2840
                     /locus_tag="PKI23_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35843.1"
                     /translation="MVAQLTPRRLRLAVLLLCALSGLVAGTLHAADDPKSDREQMQLR
                     RLQQAVRKLEQEKAQIEAEKAQMVQEKSQLDTQLQQNRDLLDESDRNRQGLAERVAVL
                     ERELAASRAENASLTQGAAQTQQALQRAATAAQRLDERLAEQRTALSACAASNEKLHG
                     YGMELLERYEKKRCSDALLQADPFTQLKRAEIENFQETYRDQLDEQKLSASPAESDGA
                     RDRQ"
     gene            2933..3628
                     /gene="phoB"
                     /locus_tag="PKI23_02510"
     CDS             2933..3628
                     /gene="phoB"
                     /locus_tag="PKI23_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNN35844.1"
                     /translation="MAAAQILVVEDDLEIQELISVNLARAGYRVQRAADAETAQNLVN
                     STLPDLILLDWGLPGLSGVDLMRRLRTNPRSRELSIIMLTARSEERDKIVGLDAGADD
                     FITKPFSPRELLARITAVLRRRLPHTSLDAIEIDGLRLDPLAHRVTVSGMEVALGPTE
                     FRLLRFLMANPERVHSRNHLLDQVWGDHAFFDDRTVDVHIRRLRVALEPSGRDRLIQT
                     VRGSGYRFSSCSS"
     gene            3771..4199
                     /gene="gspG"
                     /locus_tag="PKI23_02515"
     CDS             3771..4199
                     /gene="gspG"
                     /locus_tag="PKI23_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNN35845.1"
                     /translation="MFPRSVSKIRARGFTLLELLVVMVIIGMLAGFVAPRFFSQIGKS
                     QVKTARAQLDALGKALDQYRLDVGRYPATEQGLTALNERPAGEARWSGPYLQKAVPLD
                     PWGRPYLYRSPGEHGEYDLLSYGKDGQAGGSDEAEDITNW"
     gene            4206..5396
                     /locus_tag="PKI23_02520"
     CDS             4206..5396
                     /locus_tag="PKI23_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNN35846.1"
                     /translation="MKYQVKTLNADKGVDLIVLEASDQAQARHQAAAQGHAVLSVTPL
                     PLGGGLRSAGKARFPLLLFSQELLSLLNSGISIVEAIETLAEKEQRPAIQQTLRRILS
                     GLREGKAFSATLEEAPHSFPPLYAATLRASERTGDLGEALSRYIAYASQLETLRGRLI
                     SAAIYPLLLVVVGTLVILFLLGYVVPRFSHIYQDMGGDLPLLSRLLLEWGLAVERYWP
                     LLIGMVAALLGLAMQERVRHWMAQRLLGLLWRIPAVGERMRVFQLTRLYRTLGMLLLG
                     GIAVVPALEMVSGLLSAALQPRLDSATQAIREGRGISQAFEAGGLTTAVALRMLRVGE
                     RAGNMGEMMERIAAFHDEEMARWAEWATRLFGPLLMLLMGLMIGFIVVLMYLPIFQLT
                     EGIQ"
     gene            5396..7081
                     /locus_tag="PKI23_02525"
     CDS             5396..7081
                     /locus_tag="PKI23_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017337949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNN35847.1"
                     /translation="MGHAALDPPVVPFSPAELQAARVLMRARGCRLIEALAQAHDGPD
                     AACVARLAVTVRMKVAGIEAMHEMTPAFDLLAYGEALQRECLLFRAGTDGQGLVALLA
                     DPFDAGLRGWLEERAPERLEIRLASRGDIAAYLARHEETLRAMDGLVAMQASSRRDAA
                     AEDISLRSISEDSSPVVRLVNSTLYDALKAGASDIHLETVPSGIFIKYRIDGVLASVG
                     GIQGSENAEQVISRIKVMAELDIAERRVPQDGRFKVALAGREIDIRVSIMPSIHGEDA
                     VLRILDKQRLSAEVSGLRLESLGFDAPTLFALRRLATEPYGMLLVTGPTGSGKTTTLY
                     AAIGEINTGHDKIITIEDPVEYQLNGVLQIPVNEKKGLTFARGLRSILRHDPDKVMVG
                     EIRDGETAQIAVQAALTGHLVLTTVHANNVFDVLGRFTHMGLDPYSLVAALNGIVAQR
                     LLRVICSDCSQQSQPDAALLAMSGIDAAEEFDFRSGRGCGHCRGSGFRGRKAIAEVLL
                     LDDELREMIVTREPVRRIKEAACHKGTRLLREAGLEMVRRGETTLQELNRVVS"
     gene            7068..7886
                     /locus_tag="PKI23_02530"
     CDS             7068..7886
                     /locus_tag="PKI23_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35848.1"
                     /translation="MWSARRLRVALSTQQLVLVEHSRPWSRRPTAVQQLHYRSEGVAE
                     SAPWRPAVDALTRLLAQRQAQGAALHVVLCGRFARWQLLPWRAGLTRPQEWSAYAAVT
                     FQETFGAVTREWQVQPSLQPPQQAVPACAVDAGLLRALRTACAEAGATLATVAPYFSV
                     AFDHWRSALEGRVVWFGVLEADSLALGLLQQEQWLGLRSQRLDSDWRELLPGMMAQIG
                     IQAGLDEVETAAPRFYLTGAGEAPAADPRHPFVWLQPRSQTQFDGIGCRMALGL"
     gene            7895..8422
                     /locus_tag="PKI23_02535"
     CDS             7895..8422
                     /locus_tag="PKI23_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNN35849.1"
                     /translation="MPPLEIDFSARRRLASPLGWLLLAAGGVAMALVSLDHLAALDAL
                     RLVEVEQARLSQQREQVQPAGRAGRSELEQAELKSAQRIAAQLNLPWNALLATLERSA
                     DPNVALLSFESEGQTRQLHLMGEARTIDDVVVYAQRLEQSSQVAAVTLSRHEQRRVEQ
                     ESVIRFTLDVDWSRQ"
     gene            8419..8964
                     /locus_tag="PKI23_02540"
     CDS             8419..8964
                     /locus_tag="PKI23_02540"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HNN35850.1"
                     /translation="MITAELFTAHLRHQLHRHGWPAAAGLLLGLMALLWQLVIVQAVR
                     SETLAQQAALEALRHQRAAQSTPRQVEGRRLSDLHASLPTDSDALAVIERLHHAATLH
                     GVELPQGDYRLSRDASGTLLRYQITLPARGGYPQLRAWLADLMNALPTIALDGLAVQR
                     DDVGSGRIDARVRLTLFLKAG"
     gene            8976..9491
                     /locus_tag="PKI23_02545"
     CDS             8976..9491
                     /locus_tag="PKI23_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35851.1"
                     /translation="MAPSRSLLRQAVLALALIATLIAAFVDFGEPELVQPRQREPLAG
                     RAAGEADASSRRVTESRVPPAVREQHVAQGIDLFAAHSWQRLPLVERMPAPPRSTAPR
                     VPPLPFRYLGQLFDGRQSVLFVGQGPRSHLLREGDVLERYRLVRIAAQEATFVHLPSN
                     QRQLLKFTREN"
     gene            9491..11536
                     /locus_tag="PKI23_02550"
     CDS             9491..11536
                     /locus_tag="PKI23_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HNN35852.1"
                     /translation="MSPHAHATLRRLVLTLLLLLGGCATHPAIDESDRLIAQGHTEEG
                     VLRLAQAVQENPDDHQLQGRYLRQRERLLGQLSEAAASEREAGRIDAAEALYQRMLKL
                     DANNQRARNGLTEVAMERRHQAALAEVRVLMAQNDTAAAERILRTLVAENPLNSEAKR
                     LLQDVRRKAQQSEEPLRRALKSPFTAPISLEFHDAEMKTVFEMISRSAGINFVFDKDV
                     KSQTKVTLFVRDTSIDEVVRLILATNQLERKMLNENSVLIYPNTPAKAKEYQELVTRS
                     FYLANAEVKQAQALVKTLVKSKDIFVDEKLNLLVIKDTPEAVRMAERLLESLDIAEPE
                     VMLELEVMEMTRSKLRELGLRYPDRIGYGLLRNPNIVLQPTQGVGGQVVPEAGAEVAP
                     GVVDLSNADELTAYVTNPALVLNLRNEAGDGSLLANPRIRVKNRSRAKIHIGDKLPVF
                     TTTSTANVGVSSSVSYLDVGLKLDIEPNVYLDDEVSMKVDLEVSSVVKEVPGPAGSLA
                     YQIGSRTAGTVLRLKNGETQVLAGLISDEERSSANRLPGLGDIPVVGRLFSNQRDSGS
                     KTEIILLITPRVVRNLALPEGATAVNAAGSEAAVGAPALRVNQTGLHGLSLSSQPAGG
                     SAVVSPPLSEQPAEPVETPELPLPPPAPEPPSLPPEPAAMPALPLPAAGTPPLPEPQ"
     gene            11536..12009
                     /locus_tag="PKI23_02555"
     CDS             11536..12009
                     /locus_tag="PKI23_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNN35853.1"
                     /translation="MQRRSRGFTLLELVVTVAIIGVLASVALPFGELTVQRMKERDLR
                     SALRQIRDGLDAYRQAVDEGRIRGRADGSGYPRSLDELVAGVEDARSPSGAKIHFLRR
                     LPRDPFAAPQLPAADSWGKRAYRSPPDAPFEGEDVFDVYSRSQRIGLNGVPYREW"
     gene            11999..12403
                     /locus_tag="PKI23_02560"
     CDS             11999..12403
                     /locus_tag="PKI23_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNN35854.1"
                     /translation="MSGDRRRGFTLIELLVVMAIIATLLSIVAPRYFTHLDRAREAAL
                     RETLVVVRDAIDKFHGDTGRYPESLEELVSRRYLRTLPIDPVSESSETWILLPPPAEP
                     AEPTDPADTQAASGVWDIRSGAGGEAQPYAQW"
     gene            12390..12902
                     /locus_tag="PKI23_02565"
     CDS             12390..12902
                     /locus_tag="PKI23_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNN35855.1"
                     /translation="MRSGERLGSAAAQRGFGYLLALFAIAAIGLLLAGAGEVWSTTMQ
                     REKEAELLWIGEQFRRALSRYQQSSPEAASRAPATLQELLEDRRFPTPRRHLRQIYRD
                     PLTGQRDWGLVRLDGRIVAIHSLSSARPLKQRFDGRDADLTGKQRYSDWLFSAAVAGD
                     AAGESMRVPE"
     gene            12899..13693
                     /locus_tag="PKI23_02570"
     CDS             12899..13693
                     /locus_tag="PKI23_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNN35856.1"
                     /translation="MSDDILLSSQDREHLLFTIEASLKIGRRFQFFLWAQGVLQGVIP
                     HQTLLCAHGDLEKRQLSHLSFSADAQPDRQTGQALDPPLHDLLPRLVTLWLREGGTPC
                     LLGADALPEADVQSGRWQLLRALREADLDHVAAHGLRQMQGGQGSFFVFIGTVPYGPR
                     DRHLLELLVPHLHAALQRLLANEPDEADAAPDAIERLLTPRERQVLHWVRNGKSNKEI
                     GRLLGISPLTVKNHVQRILVKLKVNNRTQAANEGSAAKPPADETAD"
     gene            13974..14837
                     /locus_tag="PKI23_02575"
     CDS             13974..14837
                     /locus_tag="PKI23_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35857.1"
                     /translation="MNSQLKLLAAAVALAGSATAHAAMDNFISGNSSLAFVALDSVGS
                     PISVMMDLDFRLDDFLPSNQGAAGTKIVWDFNQNRLTVNGVLSTSTQANWSSALGTFQ
                     ATANSSETKWAVIGGDSLAGIGNTARYLSTSTAPLATLQNQTKFNLQGFSGVDSLYNA
                     NNLLQSAASGQNGSTATAGAAYVGTPDAFNVAGTWENKTIGWSAYALEGAAQSLYLLS
                     TDNGISSTKALAVQYALANPTGPATFHYSSGVLTYSVPAPVPLPPAVGLMAAGLGLLG
                     TLARRRAGQAG"
     gene            15042..16430
                     /locus_tag="PKI23_02580"
     CDS             15042..16430
                     /locus_tag="PKI23_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35858.1"
                     /translation="MQLKSLALAVAALSSAGLAQAYTDSATNLYLTGASAIRNNVAAA
                     IKAQCTADGGTLTVYKNGASTSSLANQMAYVCSAAMAGTPITTVLHTVTGGSLNSILG
                     MSDDAAKQQQPVSLTSGCATAVAGSGALAGYSVRASCALETAAPSDGGFSDVEYAPVR
                     EQVDVLTSDVDGFSVDEVTQGFTGVAQVFGLAVSEKLYKDLQAAQGLISAVCTAGDPL
                     PACQPSLSRADVVSLINNNVFTAHKNGAEALGLTAGAAIEYARRVTTSGTQTSAQVLF
                     LGKGCLTGSNGGELQVIGDELAAGATQGYTDQFTVSANSGTGDVISRLNAAGNSAYAF
                     GWVSGENRAPTTTTGWKFIKLNGAHFSDGTDTGLNKQQAIDGKYDYFVEAVSYKGPAA
                     KTDDAEGTLLDAISGKMATVTADGGPATVGLFIIPENAAGYSHADHPTEVSVYQRGGS
                     GPNSCQPVSRPF"
     gene            16544..18163
                     /locus_tag="PKI23_02585"
     CDS             16544..18163
                     /locus_tag="PKI23_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNN35859.1"
                     /translation="MPRGCRFDARMPRRLARWLPCALLPWSALLSAEAPAAAPLRFDV
                     LVYLVEGNSVLSTPEIERAVYPFLGPQRSVQEIEAARAALEKAYQQRGYLTVSVELPE
                     QQVENGEVRLQVVEGRVEKLRVTGARYHLPSTIREGLPSLAAGSVPQFGQVQQELADL
                     GRQPDRRLTPLLRPGKAPGTLEVELQVEEQLPLHGTLEANSKQSPNTVAGRLEGGLRY
                     DNLFQLGHTLGLNWIVAPGNPDHADILTLNYGLPFAGGDYAYAFITRSDSDVPTGVGG
                     ATVVKGTSLGARYRIDLPNRGGLFSHGFSFGVDHKDNQDDLTQAGLLVPRSLKYWSLA
                     ARYDQSRFDSGDGVTANFDLNLTLGIRGLAERTVDCDGVPTDQFACKRFNAQPNFMVW
                     RAAAEQRRPLWSGWTLRLRGEAQWANGPLVSQEQYGSGGSSSVRGYQEYEQFGDQGGL
                     LSLELGTPPFWQWSALKFSALGFVERGQAWLIDALPGEDASMALASYGLGLAAEGDGF
                     SSRFEWGMPLFSTRHTRRNDGLFYLSARGEF"
     gene            18197..28654
                     /locus_tag="PKI23_02590"
     CDS             18197..28654
                     /locus_tag="PKI23_02590"
                     /inference="COORDINATES: protein motif:HMM:NF023959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNN35860.1"
                     /translation="MNRGIHRLIFDPRRAMLVPVAESVRARRSTAAGSGRLVGLCALL
                     TLLLPAALWPRLASAARPLPSSATQAPQANLPQAAAQFNQLGRATQAVDATNPQRLLV
                     EQQDQRVILNWSSFDIGRDHSVRFNQPTGGSALNRIGGSSPSVILGSLEANGELILYN
                     ANGVLFGSGATVSSGNFIATTLNIADQQFEQGFRNITRHAPAFAAADGELEGFVRVEA
                     GASIAAASGGDIMLFAPRLLNEGRLSAPGGQVALAAGQKVYLAASLNPSERGLLVEVD
                     PFEAAVTGGDPDYNRAENAARGSTSTLSNGQLVQRVNEIVAERGSVSMVGLSVRQNGT
                     VRATTAVKGQNGAIRLLAHGKTVSASHSDGITGHTMAAASEFGELVVGPQSVTRIDAD
                     TSSATQLDAEPFYNSTVSLEGKQIHLQGGAQIIAPGAGGVTPAADAQYPETRRGVTLR
                     ASATPLSSGALGVANASSSDDSRIVIDPGVLIDVSGNDVDLPMQRNVLTGRLFSIQLA
                     DSPLQRTGVLYRQEISFDLRKGLSIADVDDFYGQIGRTAAERLTAGGRIDIRADGAVV
                     IGEGATLDFSGGSLRYAGGEIRTTQLRKGNQLVDISAARPDVVYDQIMPSTRGRYEPG
                     YVEGRNAGELTLVGRQLLLDGTLRGGVQLGRYQRGGPAGASPLAGRLQLGMVAGPAAS
                     PDHYLSSVLLAAAGGERVGADFFANPFTALLPAARTATTTLSSRMLMAGGLGRIELLA
                     NDRVQVAADSDLDLGPGGSFTATARRIEVEGRIQAAAGSITLNARPTILTGATPAPNA
                     GLSVLLAPSARLDTAGRWSNDALGTLHGTTFDAPVAVGGGSIKVAAYDSVLLAAGAAL
                     DVSAGAWRSATGKVSQGAAGAIDLAANVGNGDPLTQSGTVQLAGSVTGYGFAGGGRLT
                     LSSLEITVADLASGGLWLMPAFFSDHGFETIDLRSIDDLLIADGTRIDARLRNRVLDI
                     DAADRPSGTPISALSHFAAPGEQDEVANRPVNLNFAATRLPSASAGITGANLQVGRGA
                     SVATTAGGALSFSAGNSLTVAGTLQALGGTIDLSIEGVRGGSASDPDKIGFLPAQALW
                     LTDTARLLAGGTAEQSYSPLGLRSGVVHGGGTVTLNANRGYLLAEAGSLIDLSGHETT
                     FDLYAGRANALISRDAGSLTLASPEGILFDATLRAMAPSPHALGGRLALSISRQGLDN
                     FTEGTAYPTGERKILLQRDGTVLPAGLHPGDVLDLALGNGVAALSAAAVESAGFASVR
                     LRADDRIEFTQDVALATARALELDSRVISAQGSAAVLLEAPYLRLGDSDIQPVAGMAA
                     PTAAAGMATLTAHADLIDLVGTLGLQGFSATQLNAARHGLQQGEIRLHGAPLSGTAQR
                     GALLFAGTLDLTAGQITPTTFSTFDITGLSGDSLLRTHAPVGGSTAALPLSAFGSLRL
                     SADAIEHGGVIRAPFGRLELAATNRLELLDGSELSLAGSGLTVPVGTTVNGSSWNYFP
                     LGTAAGNAELFRTITALPVDRQVLLNGGTMAISAGARVSAAGGGAVQAAEFIAGAGGS
                     IDYLASTPGLYAVLPGYSFGYAPHDAQIQAGTDLLPGEQIVITMAGSALAPGRYTLLP
                     AAYALLPGAVLVSRAADQGSSALDRALLLSDGSRVVTGYRTVAGTAAMDDLQRYLVEP
                     AATFRAKAEYRLVSGDDFFSGQAARLGGDPGRLTRDAGRISLVANQLLDWKALFDLSA
                     PLGGRAGEFDLVLPNIVLTREGEAASAGHTAADGYTQVSIDRLMATGAGSILLGGTRS
                     GDGVVSITTGSERVRVESGLQLDELLLAARRAVEVADDVDMSATGRRDSAAGQLNLSG
                     DGALLRISNRMGVDLVRSGVARAAGFGDLLIGANTTFNAAQVEIDATGSLTLDSSARL
                     FADAFGLGAGRIAFSAASPEGDGVVIEGALLDSVRGASSLSLRSYSTIDFLDSLDLAL
                     VGSGRLLLDAASLRGLGDADDVVHLRAGEVLLRNSSGGALAAQGQGRLTIEAQPPLRD
                     RVTGGLELGGGSQALGFDQVTLSSAGDLIFSGSGQLTTLDRLDLLAQRLTTTTAADHT
                     VTAGGVLTIGRPTAGHSLNERVGLGGKLTLTGQQVFQHGTIDLPGGQISLIATNSANA
                     VQGIELSAGSITRAGGFATTLPQGGAAFGDAGWIDLIARQGAILIDGRVSVAAQAGGG
                     HAGRLSLSAIGSDGTLILGDNALLEGEAGDGGAGGSFSADLMTLPDDAADGSDGLDGV
                     AAATGDFSHLFNLRVGSGDVALNTGQVKAGQILISADAGSLTIGSTLDARAARGGVVQ
                     LSARDALTLANGGRILANSTRAGANGGDVLLSSSTGRVTVAAGAAIDAGGDDDKDGRV
                     VLRAERSGTTVKVDPIAGVIDAAEVSIEALRRYVGSSIKSGTTLGAAIGQSTLLADIT
                     SFMANKSAILTALGVATDPRFHLRPGVEVTSSADLTLTNDWNLYAASRPGGEPGFLTL
                     RAAGNLNISNNLSDGFNGLLRTSLLQAGDAWSMRLVGGADLAAANPLAVNRNPIGDLT
                     LAGGKLLRTSSGSIELAAGRDIVLGGTTTAPATVMVTGTASTLAPALAAEFTTPANAL
                     FTAHGGRLTASAGRDIRSPVSDQTLINNWFWRSGRLDAGGNFSSSSLAWWSRLDLFRH
                     GFGSFGGGNILLSAGRNLQDVGAFAPSSARMTSRIPDASKLLVENGGDIEVLAGGDIA
                     GGSFFVGRGQGLIRAAGEIGSGTALNSTIGPLAPVLALNDGGWTLRGRSDVAITAAFN
                     PTIMVPNSSNSGSFFYSYGSSATLNLASTAGDFHWQDLSSAGLNSLRALPTGTGTLNE
                     DLVSFVPVTTPTPYIAPPRVEIMAHAADVVLSGNRNNTLGISLYPSALGSLVLHAGGD
                     LFLNSSLRMMDNRPADLAGVTNPVFTATNSAILGLVGSTIGYDINKLLFTDLHQADSN
                     PVRIDARGSLNVVDGKKLIMPKQALLSAGQDILNLTLFGQHHRSTDVTLIEAGRNLLN
                     TTLLGNNGLISLAGPGRLEISAGRQLNLGASAGVETVGNLYNDTLPVQGAAVQLAASQ
                     AATLDAQGLDQFLSRYLAASGAPSGADYRNALVDQVRQTLGQGREVEYGYDDALALFR
                     GFGSEAQVAFIQQVLAQEFANSYLQPGQTYAAAWAANAAAAGVAVDHHAGSAFEQMRR
                     EVMFNEIRLAGIAGAAAKGAAAKEAGYAPGYRAIELAGHGAPFGFVGGIDLSQSKVQT
                     KSGGGIDFVNPGGGVNVGLSAEVSNVIGQAKSADQRGVVTFGGGGIRAFTDGDFLVNA
                     QKVFVIGSGDILLWSSNGNIDSGKGSNNTVTVPPLVPKVNPLDGSISFELPTLATGSG
                     IGILAAAGSSADGAAYLFAPRGEVIALDAFIRAPVIFFDPEKIRGADNALGVAPNPVS
                     APAVPTVTGLGSSATQNSEAQSKGSEAPQRSERERSSILTVEVLALGEMPAAAGGATG
                     PEPATEGDPDERKKKKSIQ"
     gene            28799..29395
                     /locus_tag="PKI23_02595"
     CDS             28799..29395
                     /locus_tag="PKI23_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNN35861.1"
                     /translation="MPEKPFSQACENNRQPIVELLQRILADCRELLEIGSGTGQHAVH
                     FAPRLPQLRWQTSDLAQHHAGISAWLAEQPAPNLLPPIELDVDRLPWPVAGKDGIFTA
                     NTCHIMGWSTVCNLFRGAQALLPSGGLLVVYGPFNYGGEFTAPSNARFDLWLKQQAPQ
                     MGIRDFEAVDRLANEAGMTLREDNAMPANNRLLVWQKR"
     gene            29541..30146
                     /locus_tag="PKI23_02600"
     CDS             29541..30146
                     /locus_tag="PKI23_02600"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNN35862.1"
                     /translation="MTRPNHLYQLILVLTLALWSAAAAATRGPAALEVDPELRSHLLK
                     VIRDEEASLFVDRFDAEVWMMDMEQRLRRFVPATQERITLLRLVHRESVRHGLRPEIL
                     LAVIEVESLFDPYAVSSSGAQGLMQVMPFWRKEIGRPHDNLTQTEINVRYGAAILAHY
                     LQRERGNLPNALARYNGSLGQAWYPRRVLSAWRKHWYLGEI"
     gene            complement(30159..31058)
                     /gene="yccX"
                     /locus_tag="PKI23_02605"
     CDS             complement(30159..31058)
                     /gene="yccX"
                     /locus_tag="PKI23_02605"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNN35863.1"
                     /translation="MTRCLHVLVSGKVQQVGFRAATRREALRLGVTGHARNLADGQVE
                     VVMSGEEEAVTALAQWLRRGPALARVDRLRVDPLPTQRFDDFVIRQQPSASPMTVAFV
                     LFDQIEPMDLAGPFEVFSTAARLHRRAHPAAPALFELITVAEQPGHVVRSRGDLLLMP
                     SHGLDQHPAIDLLLLPGGDIDALLDRTTLIDWIATTAQGAQITATVCTGAFLAAQAGL
                     LAGRRVTTHWEDCAELAARFGDLQVVEGVRYLDEGKVVTSAGVAAGIDMALHLLTRLR
                     SLHLALDTARQMAHRWRPANEQD"
     gene            31167..31565
                     /locus_tag="PKI23_02610"
     CDS             31167..31565
                     /locus_tag="PKI23_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35864.1"
                     /translation="MTEKSIVIEAIGPQGTRYQELAKGIQASFRGRGIVVQAPRTKSE
                     SSWTSVIVSVSSGIETDLLNNLIDLLTTLADGEQEKHDLSIHLEGDEVFYDLQKEKQK
                     FLTRYPQMKPKSGAVTKARSTDDLEESAIE"
     gene            complement(31603..33126)
                     /locus_tag="PKI23_02615"
     CDS             complement(31603..33126)
                     /locus_tag="PKI23_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNN35865.1"
                     /translation="MTLIRQQDLIDSVADALQYISYYHPLDFIQALHRAYQAEESPAA
                     KEAMAQILVNSRLCAEGHRPICQDTGIVNVFLKVGMQLRWEGTLSVTEMVNEGVRRAY
                     THPDNLLRGSVLADPAGARRNTRDNTPAVIHMELVPGDRLEVTVAAKGGGSENKSKLA
                     MLNPSDSILDWVVKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKESLMEPIDIDELK
                     ARGPSNRLEELRLQIFEAVNDLGIGAQGLGGLTTVLDVKIMDHPTHAASLPVAMIPNC
                     AATRHVHFTLDGSGPAELTPPRLDDWPKLVWEAGPTSRRVNLDTLTADEVASWKPGDN
                     LLLSGKMLTGRDAAHKRIADLFARGEQLPDGVDFKGRFIYYVGPVDPVRDEVVGPAGP
                     TTATRMDKFTEVMLEKTGLLGMVGKAERGQAAIDSIRAHKAVYLIAIGGAAYLVSKAI
                     RKSRVVAFADLGMEAIHEFEVEEMPVTVAVDTSGNSVHRSGPEQWQARIAAQKVTIR"
     gene            complement(33217..>33712)
                     /locus_tag="PKI23_02620"
     CDS             complement(33217..>33712)
                     /locus_tag="PKI23_02620"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35866.1"
                     /translation="VVNGVDLDLKRRLGKGAYLLTMARMVGRYGRQRYRVRSNDQQWQ
                     VGSLIISHARHYAGPFVLDPEASLFAPHLNLVMLERHTRLGLLSYLLALPTGRSSRLP
                     GVRRALGSHIHVEGAVGEPIQLDGDPHGCLPVTITLDPEPVRIAVDATHPHSHTDQHW
                     MQAI"
BASE COUNT         5565 a        11005 c        11569 g         5573 t
ORIGIN      
        1 cagggaaaac aaggagatca aggcgccgca acccaaaccg gtggaggcgc cgcaggcgcc
       61 gcaaccgctg aagatggagg gtgctgccgg ggagggttcg agcccatttg cggccggcac
      121 ggtgtcgagc gattacatcg gcggcaaggt gggcggcggt ggtggcggca tgcagttcgc
      181 gttcttttcc agtgcgctgc aacgccacat ccagcaggag ctggcgcgca accgcgcgct
      241 caagctgggc gattaccggg tgctggtgaa tgtctggatc gatgcccagg gcgtcatgcg
      301 ccgggtggaa ctggacgagt cgaccggcaa tcccgacacc gaccgcgcca ttcgggaggc
      361 gctgaaccag cttccaccgc tgcgcaccgc cgtgcccgag aatctgccgc aaccgatcag
      421 gctgcgcatc accaatcgca tggcgggctg atgcagccag tgcgaccacc gcgagaaccc
      481 aaccatgcaa aggcccggac catgacattc aagacactgc gctgcggcgt gctgctggcc
      541 tcggcgctgt tgagcgcgcc ggtgtgggcc gaagattcgc gtgcctcact ggaagcgctg
      601 cggcaaacga cgctgaatct gatcaatgca ctggtcgact ccggcgcaat cacccggcag
      661 aaggccgatg aactgatcaa aaaggcggag caggatgcag cggccacggt ggcgcagacg
      721 ccgaagagag ccgacaacac cgttcgggtg cagtacgtgc ccgaggtggt gaagagcgag
      781 atgcgtgagc agatcaagca ggaggtgctg gagcaggcca agcgtgagca ttgggccacg
      841 cccaatgcga taccggactg gctggatcgc atcaagtggg agggcgacct gcggctgcgc
      901 gagcagctcg accgtttcga cgaaaacaat gcaacgccgt ttgactacgc caccgccgcc
      961 gggaccaatg caccgccacc gctgccgaac gaccagacgc gcgctgccga gttcgccaag
     1021 gtcaatggcc agggcatccc cactgccaac accacagaag accgcgaccg cctgcggttg
     1081 cgggcaaggc tgggaatgct ggcccggctt tccgatgagt ggagcggtgg ggtgcgactc
     1141 gccaccggca gcaacagcga ccgggtgtcg accaaccaga cgctgggaca ggatttcaac
     1201 aaatacaccc ttctgctcga ccgcgcctac ctgaaattcg acccgaccga ttggctctcg
     1261 ctgaccggcg gtcgcattcc caacccctgg ttttcgaccg atctggtctg ggatgaggat
     1321 ctgaacttcg aagggctggc cgccaacttt cgacccgtgc tctccagcag cagggtcaag
     1381 cccttcgtga cgctgggcgc ctttccactt caggaagaga acccgccctc ttccagtggc
     1441 cgctggctgc gtggggtgca gggcggcacg cagtgggact tcagtccgga catgcgactg
     1501 aaggtgggac tggcctggta tgactaccag gatctggccg gggaggtcga gcctgccagt
     1561 gcgctcgaca gcaccggaaa cccgatcacc gccaattacg ggcagtatga gtatggcaac
     1621 ggactgcggc agaagggcaa ctcgctgttc gtcaccaatg cgctgggcga caccggatcg
     1681 accccctact gggggcttgc cgcggagttc cggccggtca acctgactgc atcgctggag
     1741 ctggcgcagt ttgcgccggt gcatgtggtg ttgagcgggg actgggtcaa gaacaccgcc
     1801 ttcgaccgcg cgcagatcct ctcgcgcacc caggggcgga tcaacctgac cgatggcagc
     1861 gaccagggct accagtaccg gctcaccgtc gggacggccg aggtcagaaa tcgacgggac
     1921 tggcagctgt cggtggccta tcgccatctc ggttccgatg cggtgctgga tgccttcacc
     1981 gattccgact tcgggctggg cggcaccaac ctgaagggct atctgctggg cgcgcagtat
     2041 ggactcgatc gcaacaccgt gctgaacctg cgctggctgt cggccgattc gatcgactcc
     2101 ttctcgttca ttgccgacca caagtttgcc gtcgacctgt ttcaggccga cgtgagcgtg
     2161 aagttctgac catggtggcc cagctcacac ctcgtcggct gcgtctcgcc gttctgctgc
     2221 tctgtgcgct gagcggactg gtcgccggca cgctccatgc cgctgacgac cccaagagcg
     2281 accgcgagca gatgcaattg cggcggctgc aacaggcagt gcgcaagctc gaacaggaaa
     2341 aggcgcagat cgaggcagaa aaagcacaga tggtgcagga aaaatcccaa ctcgatacgc
     2401 agttgcagca aaaccgcgac ctgctggacg agagcgatcg caaccggcag gggctggcgg
     2461 agcgcgtggc cgtgctggag cgggagctgg cggccagcag ggcggaaaat gcgtcgctga
     2521 cccagggcgc tgcccagacg cagcaggcgc tgcagcgagc ggccaccgcc gcgcaacggc
     2581 tcgatgagag gctcgcagag cagcgtacgg cgctctcggc ctgtgcggcc agcaacgaaa
     2641 agctgcatgg ctacggcatg gagctgctgg agcgctatga aaagaagcgt tgcagcgatg
     2701 cgctgctgca ggctgacccc ttcacccagt tgaagcgcgc cgagatcgaa aatttccagg
     2761 agacgtaccg cgaccaactg gatgagcaga agctgagcgc ttccccggct gaatcggatg
     2821 gcgcccgtga tcggcagtga ggccggatct gaacattttt caatctgtca ccgagctgtc
     2881 atcactgctg tctagagtgc gctgattcaa aaacatcagc ttgttcatgg acatggcagc
     2941 agcacagatt ctggtggtgg aagatgacct ggagattcag gaactgatct cggtcaatct
     3001 ggctcgagcg ggttaccggg tgcaacgtgc cgccgatgcc gagacggcgc agaatctggt
     3061 gaacagcaca ctgcccgacc tgattctgct cgactggggc ctgcccggtc tgtcgggcgt
     3121 cgacctcatg cggcgactga gaaccaatcc gcgctcgcgc gagctgtcga tcatcatgct
     3181 cacggcgcgc agcgaagagc gcgacaagat cgtcggcttg gacgcggggg ccgacgactt
     3241 catcaccaag cccttctccc cgcgtgaact gctggcccgc atcaccgccg tgttgcggcg
     3301 tcgtctgccc cacacctccc ttgacgcgat cgagatcgat ggcctgcgtc ttgatccgct
     3361 cgcccaccgg gtcacggtgt cgggcatgga ggtggccctg gggccgaccg aatttcgtct
     3421 gctgcgtttt ctgatggcca atccggagcg ggtgcacagc cgcaaccacc tgctcgatca
     3481 ggtgtggggc gaccatgcct ttttcgacga ccggacggtc gacgtgcaca tccgccggct
     3541 gcgggtcgca ctcgagccga gcggccggga tcggctgatc cagaccgtgc gtggcagcgg
     3601 ttaccgcttc tcttcctgtt cgtcctgatc gggcaggtcc gcatcggtgg tgccggagtg
     3661 ctccggtcac ctgcaagctg cataaaaatt gagcgctgcg ccggtttgtc attgttctgt
     3721 catcgatgaa gcctagcctg cgctggctct tcagccggga tccggtcgat atgtttccac
     3781 gctctgtgtc aaagatccgt gctcgcggat tcacgctgct cgaactgctg gtggtgatgg
     3841 tgatcatcgg catgctggcc ggctttgttg cgccccggtt cttctcccag atcggcaagt
     3901 cgcaggtcaa gacggcgcgg gcccagctcg atgccctcgg caaggcgctg gaccagtacc
     3961 ggctcgatgt gggtcgctac ccggcgaccg agcagggcct gactgcattg aacgagcgcc
     4021 ccgccggcga ggccaggtgg tctggcccct acctgcaaaa agccgtgccg ctggatccct
     4081 gggggcggcc ctacctctac cgctctccgg gagagcatgg cgagtacgat ctgctctctt
     4141 acggcaagga tggccaggcg ggcggcagtg acgaggccga agacatcacc aactggtgat
     4201 cgatgatgaa gtaccaggtc aagacgctga atgcagacaa gggtgtcgac ctcatcgtgc
     4261 tggaggcgag tgatcaggcg caagcccgac accaggccgc cgcacagggc catgccgtgc
     4321 tgtcggtcac ccctctgcca ctcggtggcg ggttacgcag tgccggcaag gcgcgctttc
     4381 cgctgctgct gttcagtcag gagctgctct ccctgctcaa ctccggcatc tcgatcgtcg
     4441 aggccatcga gacgctggcc gagaaggagc agcgtcccgc catccagcag acgctgcggc
     4501 gcatcctgag cgggttgcgt gaaggaaagg ccttctcggc gacgctcgaa gaggcgccgc
     4561 acagttttcc gccactctat gccgccaccc tgcgcgccag cgaacgcacc ggtgacctgg
     4621 gcgaagcgct gtcgcgctac atcgcctacg catcacaact cgagaccttg cgcggtcggc
     4681 tcatcagcgc agcgatctat ccgctgttgc tggtggtggt cgggacgctg gtcattctgt
     4741 ttctgctcgg ttatgtcgtg ccgcgcttct cgcacatcta tcaggacatg ggtggcgatc
     4801 tgccgctgct gtcgcgactg ctgctggagt ggggcttggc ggtggagcgc tactggccgc
     4861 tgttgatcgg catggtggcg gcactgctgg ggctggccat gcaggagcgg gtgcgacact
     4921 ggatggcaca gcgcctgctc ggcctgctgt ggcgcattcc agcggtcggc gagcggatgc
     4981 gggtgtttca actgacccga ctctaccgca cgctcggcat gttgctgctg ggcggcattg
     5041 ccgtggtgcc ggcgctggag atggtgagcg ggctgctctc cgccgcgctg cagccgcggc
     5101 tggacagtgc gacccaggcc atccgcgagg ggcgcggcat ctcgcaggcc ttcgaggcgg
     5161 gtgggctgac caccgcggtg gcgctgcgca tgctcagggt cggcgagcgt gccggcaaca
     5221 tgggcgaaat gatggagcgc atcgcggcct ttcacgatga agagatggcg cgctgggcgg
     5281 agtgggcgac ccggctgttc ggtccgctgc tgatgctgct gatggggttg atgatcggat
     5341 tcatcgtcgt gctgatgtac ctgccgatct tccagttgac cgagggcatc cagtgatggg
     5401 ccatgccgcg ctcgacccgc cggtcgtccc cttctcgcct gcggagctcc aggccgcccg
     5461 cgtgctgatg agggcacgcg gctgtcgcct gatcgaggcg ctcgcgcagg cgcatgatgg
     5521 cccggatgcg gcctgcgtcg cgcggctggc cgtcaccgtc cgcatgaagg tggccggcat
     5581 cgaggcgatg cacgagatga cgccggcctt cgatctgctc gcctatggcg aggcgctgca
     5641 gcgggagtgt ctgctgtttc gtgctggcac ggatgggcag gggctggtgg cgctgctggc
     5701 cgatccgttc gatgccggac tgcgcggctg gctggaggag cgggcgcccg agcggctgga
     5761 gatccggctg gccagccgcg gtgacattgc cgcctacctc gcgcgccacg aagagacgct
     5821 gcgggcgatg gatggcctgg tggcgatgca ggcatcgagc cgacgcgatg cggcggccga
     5881 ggacatctcg ctgcgcagca tcagcgaaga cagcagtccg gtggtgcgtc tggtcaactc
     5941 gacgctctac gacgcgctca aggccggcgc cagtgacatc catctggaga cggtgccgtc
     6001 gggcatcttc atcaaatacc gcatcgatgg cgtgctggcc tcggtgggtg gcattcaggg
     6061 gagcgaaaac gccgagcagg tgatctcgcg catcaaggtg atggcggagc tcgacatcgc
     6121 cgaacggcgt gtcccgcagg atggccgctt caaggtggcg ctggccggcc gcgagatcga
     6181 catccgggtc tcgatcatgc ccagcatcca tggcgaggat gcggtgctgc gcatcctcga
     6241 caagcagcgc ctctccgccg aggtgagcgg actgcgcctc gaatcgctcg gcttcgatgc
     6301 ccccacgctc ttcgcgctgc gccggctggc gaccgagccc tacggcatgt tgctggtcac
     6361 cggacccacc ggcagcggca agaccaccac gctctacgcc gccatcggcg agatcaacac
     6421 cggccatgac aagatcatca ccatcgagga tccggtcgaa taccagttga acggcgtgtt
     6481 gcagattccg gtgaatgaaa agaaggggct gaccttcgca cgcgggctgc gctccatcct
     6541 gcgccacgac ccggacaagg tgatggtcgg agagatccgc gacggcgaga cggcccagat
     6601 cgcggtgcag gcggcgctca ccggccatct ggtgctcacc accgtccatg ccaacaacgt
     6661 cttcgacgtg ctgggccgtt tcacccacat ggggctcgac ccctacagcc tggtcgcggc
     6721 gctcaacggc atcgtcgcgc agcgtctgct gcgggtcatc tgttcggact gttcgcagca
     6781 gagccagccc gatgccgccc tgctggcgat gtccggcatc gatgcggccg aggagttcga
     6841 ctttcgcagc gggcgcggct gcggccactg ccgcggcagc ggctttcgtg gacgcaaggc
     6901 gattgccgag gtgctgctgc tggacgacga gctgcgcgag atgatcgtga cccgtgaacc
     6961 ggtgcgccgc atcaaggagg ccgcctgtca caagggcacg cggctgctgc gcgaggcggg
     7021 gctggagatg gtccggcgcg gcgaaaccac gctgcaggag ctcaaccgtg tggtcagcta
     7081 gacgactgcg ggtggcgctg tcgacgcagc aactggtgct ggtcgagcac agccgaccgt
     7141 ggtcgcggcg gccgacggcg gtccagcagc tccactatcg atctgaaggc gtggcggagt
     7201 ccgcgccgtg gcgtccggct gtcgacgcgc tgacgcggct gctggcgcag cggcaggcgc
     7261 agggtgctgc gctgcatgtc gtgctctgcg gacgctttgc ccgttggcag ttgctaccat
     7321 ggcgtgccgg attgacccga ccccaggagt ggagcgccta cgccgccgtg acttttcagg
     7381 agaccttcgg tgccgtcacc cgcgagtggc aggtgcaacc gtcgttgcaa ccaccgcagc
     7441 aggccgtgcc ggcctgtgcg gtcgatgccg gacttctgcg cgcactgcgc acggcctgcg
     7501 ctgaggccgg cgcgaccctg gccacggtgg cgccctactt ttcggtcgcc ttcgaccatt
     7561 ggcgcagtgc gctcgagggc agggtggtct ggttcggcgt gctcgaagcc gactctctgg
     7621 cgctggggct gctgcaacag gagcagtggc tgggtctgcg cagccagcgg ctcgacagtg
     7681 actggcgcga actgctgccg gggatgatgg cgcagatcgg cattcaggcc ggcctcgatg
     7741 aggtcgagac tgcggcgcca cggttctatc tgaccggagc gggcgaagcc ccggccgccg
     7801 acccgcggca ccccttcgtc tggctgcaac cgcgcagcca gacccagttc gatggcatcg
     7861 gctgtcgcat ggcgctcggg ctgtgaccat gaggatgcca ccgctcgaga tcgacttcag
     7921 cgcacgtcgt cgcctggcct cgccgctcgg ctggctgctg ctggcagcgg ggggcgtggc
     7981 catggcgctg gtcagcctcg atcatcttgc ggcgctcgat gcactgcgcc tggtcgaagt
     8041 cgagcaggcg cgcctgtcgc agcagagaga gcaggtgcag ccagccgggc gcgctggccg
     8101 gagcgagctg gagcaggctg aactgaagtc ggcccagcgc atcgctgccc agctgaatct
     8161 gccgtggaac gcactgctgg ccacgctcga gcggagtgcc gaccccaatg tggcgctgct
     8221 ctcattcgaa agcgaaggac agacgcgcca gttgcacctg atgggcgagg cgcggacgat
     8281 cgatgacgtg gtggtctatg cgcagcggct ggagcagtca tcgcaggttg ccgcggtgac
     8341 actctcgcgt catgaacagc ggcgagtgga gcaggagagc gtgatccggt tcacgctgga
     8401 tgtcgactgg agccgccagt gatcaccgcg gagctgttca ctgcccacct tcggcatcaa
     8461 cttcaccggc acggctggcc ggcggcggcc ggactgctgc tgggcctgat ggccctcctc
     8521 tggcagcttg tcatcgtgca ggccgtgcgc agtgagacgc tggcgcagca ggccgcgctg
     8581 gaggcgctgc ggcaccagcg cgcggcgcag tcgacccccc ggcaggtgga gggccgccgg
     8641 ttgagcgatc tccacgccag cctgccgacc gattcggatg cactggccgt catcgaacgc
     8701 ctgcaccatg ccgccaccct ccatggcgtc gagctgcccc agggcgacta ccggttgagc
     8761 cgcgatgcca gcggcaccct gctgcgctac cagatcaccc tgccggcacg cggtggctat
     8821 ccgcaactgc gcgcctggct ggccgatctg atgaatgcgc tgccgaccat cgcgcttgat
     8881 gggttggccg tccagcgtga cgacgtcggc agtggccgga tcgacgccag agtgcggctg
     8941 accctgtttc tgaaggcggg ctgagcatgg caacggtggc accctcccga tcgctgctgc
     9001 ggcaagctgt tttggcactg gcgttgatcg ccacgctgat cgctgccttc gtcgacttcg
     9061 gtgaaccgga gctggtgcaa ccacgacagc gtgagccgct cgccgggcgc gccgccggcg
     9121 aggccgatgc ctcgtcccgg agagttacgg agtcgagggt tccgcctgcc gtacgggaac
     9181 agcatgtcgc ccagggcatc gacctcttcg cggcccacag ctggcaacgc ctgccgctgg
     9241 tcgagcggat gccggcaccg ccgcggtcca ccgcacccag ggtgccaccg ttgccgttcc
     9301 gttacctcgg ccaactgttc gacggccggc agagcgtgct cttcgtcggc caggggccgc
     9361 gcagccatct gctgcgcgag ggcgatgtgc tggagcgcta ccggttggtg cgcatcgcag
     9421 cgcaggaggc caccttcgtc catctgccca gtaaccagcg acaactgctg aaattcacca
     9481 gagaaaattg atgtcacctc acgcgcacgc cacgcttcgc cgcctcgtgc tgaccctgtt
     9541 gctgctgctg ggcggctgtg cgacccaccc ggcgatcgac gagagcgatc gactgatcgc
     9601 gcaggggcac accgaagagg gggtgctgcg gctggcgcag gcggtgcagg aaaatcccga
     9661 cgaccatcaa ttgcaggggc gctatctgcg tcagcgtgag cggctgctcg gccagttgtc
     9721 cgaagctgcg gcttccgagc gcgaggcagg ccggatcgat gcggccgaag cgctctacca
     9781 gcgcatgctg aagctcgatg ccaacaacca gcgcgcccgc aacggcctga ccgaggtcgc
     9841 gatggagcgt cggcaccagg ccgccctcgc cgaggtcagg gtcctgatgg cccagaacga
     9901 caccgcggcc gccgaacgca tcctgcgtac gctggtggcc gagaatccgc tcaacagcga
     9961 agcgaaacgg ctgttgcagg atgtgcggcg caaggcgcag cagagcgagg agccgctcag
    10021 gcgcgcgctc aagtcaccct tcaccgcacc gatcagcctg gagtttcacg acgccgagat
    10081 gaagacggtg ttcgagatga tctcgcgcag cgccgggatc aacttcgtct tcgacaagga
    10141 cgtcaagtcg cagaccaagg tgacgctgtt cgtgcgcgac acctcgatcg atgaggtggt
    10201 gcggctgatt ctcgccacca accagctcga gcgcaaaatg ctcaacgaga actcggtgct
    10261 gatctacccc aacaccccgg ccaaggccaa ggagtatcag gagctggtga cccgcagctt
    10321 ctatctggcc aatgccgagg tgaagcaggc gcaggcgctg gtgaagacac tggtcaagag
    10381 caaggacatc ttcgtcgatg agaagctcaa cctgctggtg atcaaggaca cccccgaggc
    10441 ggtgcggatg gccgaacggc tgctcgaatc gctcgacatt gccgagcccg aggtgatgct
    10501 ggagctcgag gtgatggaga tgacccgcag caagctgcgc gagctggggc tgcgttaccc
    10561 cgaccggatc ggctacgggt tgttgcgcaa ccccaacatc gtgttgcagc ccacccaggg
    10621 ggtcggcggg caggtggtac cggaggccgg ggccgaggtg gcgcctggcg tggtcgatct
    10681 gagcaatgcc gatgaactga ccgcttatgt gaccaacccg gcgctggtgc tcaacctgcg
    10741 caacgaggcc ggcgatggca gcctgctggc caatccgcgc atccgggtca agaaccgctc
    10801 gcgggccaag atccacatcg gcgacaagct gccggtcttt accaccacct cgaccgccaa
    10861 tgtcggcgtc tcctcctcgg tctcctacct cgacgtcggg ctcaagctcg acatcgagcc
    10921 caacgtctat ctcgacgacg aagtctcgat gaaggtcgac ctcgaagtga gcagcgtcgt
    10981 caaggaggtg ccggggcccg ccggctcact ggcctatcag atcggcagtc gcactgccgg
    11041 caccgtgctg cggctgaaga atggcgagac ccaggtgctg gccggcctga tcagcgacga
    11101 ggagcgcagc agcgccaacc ggctgccggg cctcggcgac atcccggtcg tcggccggct
    11161 cttctccaat cagcgcgaca gtggcagcaa gaccgagatc atcctgctga tcaccccacg
    11221 ggtcgtgcgc aatctggcac tgccggaggg cgccacggcg gtgaacgcag cgggctccga
    11281 ggccgcggtg ggcgccccgg cactgcgggt gaaccagacg gggctgcatg gcctgtcact
    11341 ctcatcgcag ccggcgggcg gctctgccgt ggtgtcgccg ccgctgtcag agcagccggc
    11401 cgaaccggtc gagacgcccg agctgccact gccgccaccc gcccccgaac cgccgtcact
    11461 gccgccggaa ccggccgcca tgccggcgct gccactgccc gcggcgggga cgccgccact
    11521 gccggaaccg cagtgatgca gcgccgcagc cggggcttca cgctgctcga actggtggtg
    11581 acggtggcga tcatcggcgt gctggcctcg gtggcgctgc cctttggcga actgaccgtg
    11641 cagcggatga aggagcgtga tctgcgcagt gcgctgcggc agattcgtga cggtctcgat
    11701 gcctaccgcc aggcggtgga tgaagggcgc atccgcggcc gggccgacgg ctccggctat
    11761 ccccgttcgc tcgatgagct ggtcgcaggg gtcgaggatg ccaggagccc cagcggggcg
    11821 aagatccatt ttttgcgccg cctgccgcgc gatccctttg ccgcgcccca gctgccggcg
    11881 gccgacagct ggggcaagcg tgcctaccgc agtccacccg atgcaccctt cgagggcgag
    11941 gatgtgttcg atgtctactc gcgttcgcag cggatcggcc tgaacggggt gccctaccgt
    12001 gagtggtgac cggcgccgtg gcttcacgct gatcgaactg ctggtggtga tggcgatcat
    12061 cgccacgctg ctcagcatcg tcgcgccgcg ctacttcacc cacctcgacc gggcgcgcga
    12121 ggcggcgctg cgcgagacgc tggtggtggt gcgcgatgcg atcgacaagt ttcatggcga
    12181 caccggccgc tacccggaga gtctcgaaga gctggtgagc cggcgctatc tgcgcacgct
    12241 gccgatcgat ccggtcagcg agagcagcga gacctggatc ctgctgccgc caccggccga
    12301 gcccgccgag ccgaccgatc cggccgacac gcaggccgcc agcggtgtct gggacatccg
    12361 cagtggcgcc ggcggcgagg cgcagcccta tgcgcagtgg tgagcggttg ggctcggctg
    12421 cggcccagcg cggcttcggc tatctgctgg cgctgtttgc gattgccgcc atcggcctgc
    12481 tgctggccgg tgccggcgag gtctggtcca cgacgatgca gcgcgagaag gaggccgagc
    12541 tgctctggat cggcgagcag ttccgtcggg cgctcagccg ttaccaacag agctcgcccg
    12601 aggcggccag ccgcgctccg gcgacgctgc aggagctgct ggaggatcgc cgctttccga
    12661 cgccacgccg tcatctgcgg cagatctacc gcgatccgct gaccggtcag cgcgactggg
    12721 ggctggtccg gctcgatggc cgcatcgtcg ccatccacag cctgtcgagc gcacggccgc
    12781 tgaagcagcg cttcgacggg cgcgatgccg acctgaccgg caagcagcgc tacagcgact
    12841 ggctgttcag cgccgctgtg gccggtgacg ccgccggcga gtcgatgagg gtgccagagt
    12901 gagtgacgac atcctgctca gcagtcagga ccgcgagcac ctgctgttca ccatcgaggc
    12961 ctcgctgaag atcggtcggc gcttccagtt ctttctctgg gcgcaagggg tgctgcaggg
    13021 agtgattccc catcagacgc tgctgtgcgc ccatggcgac ctcgagaagc gccaactcag
    13081 ccacctgagc ttctctgccg atgcccagcc cgaccgtcag accgggcagg cgctcgaccc
    13141 gccgctgcat gacctgctgc cccggctggt cacgctctgg ctgcgcgagg ggggcacgcc
    13201 ctgtctgctc ggtgccgacg cgctgcccga ggccgatgta cagagcggac gctggcaact
    13261 gctgcgtgcg ctgcgggagg ccgatctcga ccatgtcgcc gcccatggcc tgcggcagat
    13321 gcagggcggg cagggcagct tcttcgtctt catcggcact gtgccctatg gcccgcgcga
    13381 ccgccacctg ctcgaactgc tggtgccgca cctgcacgcc gcgctgcagc ggctgctggc
    13441 caatgagccc gacgaggcgg acgccgcgcc cgatgccatc gagcggctgc tcaccccgcg
    13501 tgagcggcag gtgttgcact gggtgcgcaa cggcaagagc aacaaggaga tcgggcggct
    13561 cctcggcatc agtccgctga cggtcaagaa ccatgtgcag cggattctgg tcaagctcaa
    13621 ggtcaacaac cgcacccagg ccgccaacga aggcagtgcg gcgaagccgc cagccgacga
    13681 aacggccgat tgaacccgcc caatagtcca tatggactat ttttgggcca tcacgcctta
    13741 acgaccctgt cacaacggcg ccttagagtg gtggagtcgg aggagggtgc gtgcagcgac
    13801 gccggccgca gcggaaccgg gccaaatgga ctatctccgc agcggcagcg cgaccgcaaa
    13861 acggtaactt tcgtcggcta tcgtgctgct gtctccacac ggaggccccc atgaatcaag
    13921 tgaagacgat ttttgcgatc tgtcaaaccc aactgctcaa aggagatcaa ccgatgaact
    13981 cacaactcaa actgctggcg gccgcggtgg cgctggccgg ctccgccacc gcccatgcgg
    14041 cgatggacaa cttcatcagc ggcaacagca gcctggcctt cgtcgcgctc gactcggtcg
    14101 gctcgccgat ctcggtgatg atggacctcg acttcaggct cgacgacttc ctgccgtcca
    14161 accagggcgc cgccggcacc aagatcgtct gggacttcaa ccagaacagg ctgacggtca
    14221 atggcgtgct gtcgaccagc actcaggcca actggagcag cgcccttggc accttccagg
    14281 ccactgccaa cagcagcgag accaaatggg cggtgatcgg cggtgattcg ctggccggta
    14341 taggtaacac ggctcgctat ctgagcacca gcacggcgcc gctggcgacg ctgcagaacc
    14401 agaccaaatt caacctgcag ggtttctccg gcgtcgacag cctgtacaac gccaacaacc
    14461 tgctgcagag cgccgccagt ggccagaatg gcagcacggc cacggccggc gccgcctatg
    14521 tgggcacccc tgatgccttc aacgtcgcgg gcacctggga gaacaagacc attggctgga
    14581 gtgcctatgc actggagggc gccgcgcagt cgctctacct gttgagcacc gacaacggca
    14641 tcagctcgac caaggcattg gcggtgcagt atgcgctggc caacccgacc ggcccggcga
    14701 ccttccatta cagcagcggc gtgctgacct actcggtgcc ggcaccggtg ccgctgccgc
    14761 cggcggtcgg tttgatggcg gccggcctcg gcctgctcgg cacgctggcg cgccgccgcg
    14821 ccggccaggc gggctgaccc cgacctgagc cgttcgctgc caccgccggg gtgcggtggc
    14881 agcggaccct cctcgacgcc tctgctgcgc ggtcgccacc tctggcgtca ccgcaggttg
    14941 cggtcgcgct gaagaagccg ataagaagtc gattcgcctc tcatgtcttt tgtgctttga
    15001 ctttgaccat gttcttgaac ccaccacagg agaaaacgac catgcaactg aaatccctcg
    15061 ccctcgccgt cgccgccctc tcttctgccg ggctggcgca ggcctacacc gacagcgcca
    15121 ccaacctcta cctgaccggc gcctcggcga tccgcaacaa cgtcgccgcc gcgatcaagg
    15181 cgcagtgcac tgccgatggc ggcacgctga cggtctacaa aaatggcgca tcgaccagct
    15241 cgctggccaa ccagatggcc tatgtctgct cggcagcgat ggccggcacg ccgatcacca
    15301 cggtgctgca caccgtcacc ggcggctcgc tgaactcgat tctgggcatg tccgacgacg
    15361 ccgccaagca gcagcagcca gtgtcgctga cctcgggctg cgcaacagcg gttgccggca
    15421 gtggtgcgct ggccggctac agcgtgcgcg ccagctgcgc gctcgagacg gcggccccct
    15481 ctgacggtgg cttctccgat gtcgagtacg cgccggtgcg cgagcaggtc gatgtgctga
    15541 ccagcgatgt cgatggcttc agcgtcgacg aggtgaccca gggcttcacc ggggtggccc
    15601 aggtgttcgg cctggcggtc agcgagaagc tgtacaagga tctgcaggcg gcgcaggggt
    15661 tgatcagcgc cgtctgcacc gccggcgatc cgctgccagc ctgccagcca tcgctgagcc
    15721 gcgccgacgt ggtgtcgctg atcaacaaca atgtgttcac cgcccacaag aatggcgccg
    15781 aggcgctcgg gctgactgcc ggcgccgcga tcgagtatgc ccgccgcgtc accacctccg
    15841 gcacccagac ttcggcgcag gtgctctttc tcggcaaggg ctgcctgacc ggcagcaacg
    15901 gcggtgagtt gcaggtgatc ggcgatgagc tcgccgccgg ggccacccag ggctacaccg
    15961 accagttcac ggtgtcggcc aactccggca ccggtgacgt gatcagccgg ttgaacgccg
    16021 ccggcaacag tgcctacgcc ttcggctggg tctcgggcga gaaccgcgcg ccgaccacca
    16081 ccaccggctg gaagttcatc aagctgaatg gtgctcattt ctcggatggc accgacaccg
    16141 gcctcaacaa gcagcaggcc atcgatggca agtatgacta cttcgtcgag gcggtcagct
    16201 acaaggggcc ggcggccaag accgacgacg ctgaaggcac gctgctcgac gccatcagcg
    16261 gcaagatggc gacggtgacc gccgatggcg gcccggccac cgtcggcctc ttcatcatcc
    16321 ccgagaatgc ggccggctac agccatgccg accacccgac cgaggtctcg gtctaccagc
    16381 gcggtggctc gggccccaac tcctgccagc cggtcagccg gccgttctga gcgactcgtg
    16441 cgtgatctga cggccggggg ttgaccccgg ccgggcgggc gcgacccggc aacggcctct
    16501 tctgcaatgg aaggggccgt tgcatttttc tggagggtgt ttcatgccgc gaggctgtcg
    16561 attcgatgca cggatgcccc gccggttggc gcgctggctg ccgtgcgcgc tgctgccgtg
    16621 gagcgcgctg ctgtccgccg aggcgccggc cgcagcgcca ttgcgttttg atgtgctggt
    16681 ctatctggtc gagggcaaca gcgtgctgtc gacgccggag atcgagcggg cggtctaccc
    16741 gtttctgggt ccgcagcgca gcgtgcagga gatcgaagcc gcccgcgcgg cattggagaa
    16801 ggcctatcag cagcgcggct acctgaccgt cagcgtcgaa ctgccggagc agcaggtgga
    16861 gaatggcgag gtgcggttgc aggtggtcga gggccgggtg gagaagctgc gcgtgaccgg
    16921 ggccagatac cacctgccga gcacgattcg cgaaggcttg cccagtctgg ccgccggcag
    16981 cgtgccgcag ttcggtcagg tgcagcagga gctggccgat ctggggcggc agcccgaccg
    17041 gcggctgacg ccactgctgc gaccgggcaa ggcgccgggc acgctggagg tggagctgca
    17101 ggtcgaagag cagttgccgc tgcatggcac gctggaggcc aacagcaagc agagccccaa
    17161 cacggtggcc ggacggctcg aaggggggct gcgctatgac aacctgttcc agcttggcca
    17221 caccttgggg ctcaactgga tcgtggcgcc gggcaatcca gaccatgccg acattctgac
    17281 gctcaactac gggctgccgt tcgcgggggg cgattacgcc tatgccttca tcacccgctc
    17341 cgacagcgat gtgcccaccg gtgtcggcgg cgccaccgtg gtcaagggca cctcgctggg
    17401 ggcgcgctac cgcatcgacc tgcccaaccg cggcggcctg ttcagccacg gcttcagctt
    17461 cggggtcgac cacaaggaca accaggatga cctgacccag gccgggctgc tggtgccgcg
    17521 ctcgctcaaa tactggtcgc tggcggcgcg ttatgaccag agccgtttcg acagtggcga
    17581 cggcgtcacc gccaacttcg acctcaatct gacgctcggc atccgcggtc tggccgagcg
    17641 cacggtcgac tgcgacgggg tgccgaccga tcagttcgcc tgcaagcgct tcaatgccca
    17701 gcccaacttc atggtctggc gcgctgccgc cgagcagcgg cgcccgctct ggagcggctg
    17761 gacgctgcgg ctgcgcggcg aggcgcagtg ggccaacggg ccactggtgt cgcaggagca
    17821 gtatggcagc ggcggcagca gttcggtgcg cggctatcag gagtatgagc agtttggcga
    17881 ccagggcggc ctgctgagtc tggagctggg cacgccaccc ttctggcagt ggagcgcgtt
    17941 gaaattcagc gcgctcggtt tcgtcgagcg cggtcaggcc tggctgatcg acgcgctgcc
    18001 cggcgaggat gccagcatgg cactcgccag ctatggcctg gggctggcgg ccgagggcga
    18061 cggcttcagc agccggttcg aatggggcat gccgctcttt tccacccgtc acacccgccg
    18121 caacgatggg ctgttctacc tgtcggcccg gggcgaattt tgacgatgac ggccgaacac
    18181 cacggcagga gctagcatga accgaggcat ccaccggctg attttcgacc cgcggcgcgc
    18241 catgctggtg ccagtggcgg agtcggtgcg cgcccgcaga tccacggcgg ccggcagcgg
    18301 tcggctggtg gggctctgcg cgctgctgac cctgctgttg ccggccgcgc tctggccgcg
    18361 gctggccagc gcggcccggc cattgccgag ttcggcgacc caggcacccc aggccaacct
    18421 gccgcaggcg gcggcccagt tcaaccagct cggacgggcc acccaggccg tggatgcgac
    18481 caacccgcag cggctgctgg tcgagcagca ggatcagcgg gtgattctca actggtcgag
    18541 tttcgacatc ggccgggacc acagcgtcag gttcaatcag ccgaccggcg gctcggcgct
    18601 caaccgcatc ggcggcagct cgccgagcgt cattctgggg tcgctcgaag ccaatggtga
    18661 actgattctc tacaacgcca atggcgtgct gttcggcagt ggcgccaccg tcagcagcgg
    18721 caacttcatc gccaccacgc tgaacatcgc cgatcagcag ttcgagcagg gttttcgcaa
    18781 catcacccgc catgcgccgg ccttcgccgc ggccgacggc gagctggagg gtttcgtgcg
    18841 ggtcgaggcc ggcgccagca tcgctgcggc cagtggcggc gacatcatgc tgttcgcgcc
    18901 acggctgctc aacgaggggc ggctgagtgc accgggcgga caggtggcat tggccgccgg
    18961 gcagaaggtc tatctggcgg cgtcgctcaa cccctccgag cgcggcctgc tggtcgaggt
    19021 cgatccgttc gaggccgcgg tcaccggcgg tgatccggac tacaaccggg ccgagaatgc
    19081 ggcccggggt tcgaccagta cgctgagcaa tggccaactg gtgcagcggg tcaatgagat
    19141 cgtcgccgag cgtggcagcg tcagcatggt ggggctgtcg gtgcggcaga acggcacggt
    19201 gcgggccacc accgcggtga agggacagaa tggcgcgatc cgtctgctgg cccacggcaa
    19261 gaccgtcagt gccagccaca gcgatggcat caccggccac acgatggcgg ccgccagcga
    19321 attcggcgaa ctggtggtcg ggccgcagag cgtgacccgg atcgatgcag ataccagcag
    19381 tgccacccag ctcgatgccg aacccttcta caactcgacc gtcagcctgg aaggcaagca
    19441 gatccatctg caagggggcg cgcagatcat cgcgcccggt gccggcggcg tgacgccggc
    19501 ggccgatgcc cagtatccgg aaacgcggcg cggcgtcacg ctgcgtgcca gcgcgacgcc
    19561 gctcagctcg ggcgcactgg gggtggccaa tgccagcagc agtgacgaca gccgcatcgt
    19621 catcgatccc ggcgtgctga tcgatgtgtc gggcaacgat gtcgatctgc cgatgcagcg
    19681 caacgtactg accgggcggc tgttctcgat ccagttggcc gactcgccgc tgcaacgcac
    19741 cggcgtgctc taccggcagg agatctcctt cgacctgcgc aaggggctgt cgattgccga
    19801 tgtcgacgac ttttacggac agattggccg cacggcggcc gagcggctga ccgcgggtgg
    19861 ccgcatcgac atccgggcgg atggcgcagt ggtgatcggt gagggggcga cgctcgactt
    19921 ctccggcggc agcctgcgtt atgccggtgg cgagatccgc accacgcagt tgcgcaaggg
    19981 caaccagctg gtcgacatca gcgccgcccg gcccgacgtg gtctatgacc agatcatgcc
    20041 gtcgacgcgg ggccgttatg aacccggcta tgtcgagggc aggaacgccg gcgagctgac
    20101 gctggtcgga cggcaactgc tgctggatgg caccctgcgc ggcggcgtgc agttgggacg
    20161 ttatcagcgc ggcggtccgg ccggcgccag cccgctggca ggtcgcctgc aactgggcat
    20221 ggtggcggga cccgccgcat cgccggacca ctatctgagc agcgtgctgc tggctgcggc
    20281 cggtggcgag cgcgttgggg ccgacttttt cgccaatccg ttcacggcgc tgctgccggc
    20341 cgcgcgcacc gccaccacca ccctctcgtc ccggatgctg atggcgggcg ggctgggtcg
    20401 catcgagctg ctggccaatg atcgggtgca ggtcgccgcc gacagcgacc tcgatctcgg
    20461 tccaggtggc agcttcaccg ccacggcccg gcgcatcgag gtggagggtc gcatccaggc
    20521 cgcagcgggg agcatcaccc tcaatgcccg gccgacgatt ttgaccggcg ccacgccggc
    20581 gccgaacgcc gggctctcgg tgctgctggc gccgtcggcc cggctcgaca ccgcagggcg
    20641 ctggagcaac gatgcgctgg gcacgctgca cgggacgacc ttcgatgccc cggtggcggt
    20701 cggcggtggg tcgatcaagg tggcggctta cgattcggtg ctgctggcgg cgggtgcggc
    20761 gctcgatgtg tcggcggggg cctggcgcag tgccaccggc aaggtcagcc agggcgcggc
    20821 cggcgccatc gatctggccg ccaatgtcgg caatggcgat ccattgacgc agagcggcac
    20881 ggtgcaactg gcgggcagcg tcaccggcta cggttttgcg ggtggggggc ggttgacgct
    20941 ctcatcgctg gagatcacgg tggccgatct ggccagcggc gggctgtggc tgatgccggc
    21001 attcttctcc gatcacggtt tcgagacgat cgacttgcgg tcgatcgatg atctgctcat
    21061 cgccgatggc acccggatcg acgcccgctt gcgcaaccgg gtgctcgaca tcgatgccgc
    21121 cgatcgcccc agcggcacgc cgatcagtgc gctcagccac tttgccgcgc cgggcgagca
    21181 ggatgaggtg gccaacaggc cggtcaatct gaattttgcc gccacccgtc tgccctcggc
    21241 ctcggccggc atcaccggtg ccaatctgca ggtcggtcgc ggtgccagcg tggccaccac
    21301 ggccggtggc gcattgagct tcagcgccgg caacagcctg accgtggccg gcacgctgca
    21361 agccttgggc ggcaccatcg atctgtcgat cgagggggtg cgcggtggct cggccagcga
    21421 tccggacaag atcggctttc tgccggcaca ggcgctctgg ctgaccgaca ctgcacggct
    21481 gctggccggc ggcaccgccg aacagtccta cagtccgctc ggcctgcgca gcggcgtggt
    21541 ccatgggggc ggcacggtga ccttgaacgc caaccgtggc tatctgctgg ccgaagccgg
    21601 ttcgctgatc gacctcagtg gccatgagac gaccttcgac ctttacgctg gcagagcgaa
    21661 tgcactgatc tcgcgggatg ccggcagtct caccctggcc tcgcccgaag gcattctgtt
    21721 cgacgccacg ctgcgggcga tggcgcccag cccgcatgcg ctgggcggcc gcctggcgct
    21781 gtcgatcagt cggcaggggc tggacaactt caccgagggc accgcctacc cgaccggcga
    21841 gcgcaagatc ctgctccagc gtgacggcac cgtgctgccg gccgggctgc atccgggcga
    21901 tgtgctcgat ctggccctcg gcaatggcgt ggccgcgctg tcggccgcag cggtggaaag
    21961 cgccgggttc gcctcggtca gactgcgggc cgatgaccgc atcgaattca cccaggatgt
    22021 cgcactcgcc acggcgcgtg cactggaact cgacagccgg gtcatctctg cccagggcag
    22081 cgcggcggtg ctgctggagg cgccctatct acggcttggc gacagtgaca ttcagccggt
    22141 ggccggcatg gccgcgccga cggcggctgc gggcatggcg acattgacgg cccatgccga
    22201 cctgattgat ctggtgggca cgctgggttt gcagggcttc tctgccaccc agctcaatgc
    22261 cgcgcgccac ggcctgcagc agggtgagat tcgcctgcat ggcgcgccgc tcagcggcac
    22321 ggcacagcgc ggtgcactgc tgtttgccgg cacgctcgat ctgaccgccg ggcagatcac
    22381 gccgaccacc ttctccacct tcgacatcac cggattgtcc ggtgactcgc tgctgcgcac
    22441 ccatgcgcca gtcggcggtt cgaccgcggc actgccgctg tcggcctttg gcagcctcag
    22501 gttgagcgcg gacgccatcg agcatggcgg tgtcattcgc gcgccctttg gccgactgga
    22561 gctggccgcg accaaccggc tggagctgct ggacggcagc gagctgtcgc tcgctggcag
    22621 cggtctgacc gtgccggtgg gcaccacggt gaatggcagc agctggaact acttcccgct
    22681 gggaacggct gccggcaatg cggagctgtt ccgcaccatc acggcgctgc cggtcgaccg
    22741 gcaggtgctg ctgaatggcg gcacgatggc catctctgct ggggcgcggg tctcggcggc
    22801 cggtggcggc gcggtgcagg cggcggagtt catcgccggc gccggtggtt cgatcgacta
    22861 tctggccagc acgccgggcc tctatgcagt gctgcccggt tacagcttcg gctatgcgcc
    22921 gcacgatgcc cagattcagg ccggcaccga cctgctgccg ggtgagcaga tcgtcatcac
    22981 catggcgggc tcggcgctgg cgcccggccg ctacacgctg ttgccggcgg cctacgccct
    23041 gctgcccggc gccgtgctgg tctccagggc ggccgatcag ggcagcagtg cgctcgaccg
    23101 ggcgctgttg ctgagcgatg gcagccgggt ggtgaccggc tatcgcacgg ttgctggtac
    23161 ggcggcgatg gacgatctcc agcgctatct ggtcgagccg gccgcgacct ttcgtgccaa
    23221 ggcggaatat cggctggtca gcggcgatga ctttttcagc gggcaggcgg cccgactcgg
    23281 tggcgatccg ggtcgcctga cgcgggatgc cggacgcatt tcactggtgg ccaaccagtt
    23341 gctcgactgg aaggcgctgt tcgatctctc cgcaccactg ggtggccggg ccggggagtt
    23401 cgatctggtg ctgccgaaca tcgtgctgac gcgggagggt gaagcggcgt cggcagggca
    23461 cacggcggcc gatggttaca cgcaggtgag catcgacagg ctgatggcca ccggcgccgg
    23521 cagcatcctg ctggggggta cgcgcagcgg tgatggggtc gtctccatca ccaccggcag
    23581 tgagcgggtg cgtgtcgaga gtggcctgca gctcgatgag ctgctgctgg cggcgcgcag
    23641 ggcagtggag gtggccgatg acgtcgacat gagtgccacc ggccgccgcg acagcgcagc
    23701 cgggcagttg aatctgagcg gtgatggcgc gctgctgcgc atctccaacc gcatgggcgt
    23761 cgacctggtg cgcagcgggg ttgcgcgtgc cgctggcttc ggtgatctgc tgatcggcgc
    23821 gaacacgacc ttcaacgcag cgcaggtgga gatcgacgcg accggcagcc tgacgctcga
    23881 cagcagtgcc cgcctctttg ccgacgcctt tggcctgggt gccggacgca tcgccttctc
    23941 ggccgcttcg cccgagggcg atggcgtggt catcgagggg gcgctgctcg actcggtgcg
    24001 tggcgccagc agcctctcgc tgcgcagtta cagcaccatc gattttctcg acagcctcga
    24061 tctggcgctg gtgggcagtg gccgtctgtt gctggatgcc gcgtcgctgc gcggtctggg
    24121 cgatgcagat gacgtggtgc atctgcgtgc gggcgaggtc ctgctgcgca acagttcggg
    24181 cggggcgttg gcggcacagg gacagggcag gctcaccatc gaggcgcagc cgccgctgcg
    24241 tgaccgggtc accggtgggc tcgaactcgg cggtggcagc caggcgctcg gcttcgatca
    24301 ggtcacattg agttcggcgg gtgacctgat cttctccggc agcggccagc tcaccacgct
    24361 ggatcggctc gatctgctgg cgcagcggct caccaccacc actgccgccg accacaccgt
    24421 cacggccggc ggtgtgctga ccatcggcag gccgaccgcg ggccacagcc tgaatgagcg
    24481 cgttggcctc ggtggcaagc tgacattgac cgggcagcag gtgtttcagc atggcaccat
    24541 cgacctgccc ggcgggcaga tttcgctgat cgcgaccaac agtgccaatg ccgtgcaggg
    24601 catcgaactg tcggccggtt cgatcacccg tgccggtggc ttcgccacca ccctgccgca
    24661 gggtggggcg gcattcggcg atgccgggtg gatcgatctg atcgcccggc agggcgccat
    24721 cctgatcgat ggcagggtca gtgtcgcggc ccaggcgggg ggtggccatg cagggcgact
    24781 gagcctctcg gcgatcggca gtgatggcac gctgattctg ggcgacaacg cactgctcga
    24841 aggcgaggcc ggtgacggcg gcgcgggtgg gagcttctct gccgacctga tgacgctgcc
    24901 ggatgatgcc gccgacggca gcgatggact cgatggagtc gctgccgcga ccggcgactt
    24961 cagccacctc ttcaacctgc gggtcggcag cggcgatgtg gccctcaata ccgggcaggt
    25021 gaaagccggg cagattctga tttccgccga tgccggcagc ctgaccatcg gctcgaccct
    25081 cgatgcccgt gccgcccggg gcggggtggt gcaactgagc gcccgcgatg cgctcacgct
    25141 ggcgaacggc ggacgcatcc tggccaactc gacgcgcgcg ggggcgaatg ggggtgatgt
    25201 gctgctctct tccagcacgg gccgggtcac tgtcgccgcc ggcgccgcca tcgatgccgg
    25261 cggcgatgac gacaaggatg gccgcgtggt gttgcgggcc gaacgcagtg gcacgacagt
    25321 caaggtcgat ccgattgccg gcgtgatcga tgcggccgag gtttcgatcg aggcgctcag
    25381 gcgctatgtc ggcagttcga tcaagagcgg caccaccctg ggtgcggcca ttggccagag
    25441 cacgctgctg gcggacatca ccagcttcat ggcgaacaag agcgcgattc tgaccgcgct
    25501 gggggtggcc accgatccgc gctttcatct gcggccggga gtggaggtca cctcaagcgc
    25561 tgatttgacg ctgacgaatg actggaacct ctacgccgcg agccgtcccg gcggtgaacc
    25621 cggctttctg acgctgcgcg cggccggcaa tctcaatatc agcaacaacc tcagcgatgg
    25681 tttcaatggg ctgctgcgca cttcgctgct gcaggcaggg gatgcctggt cgatgcggct
    25741 ggtgggcggt gccgatctgg ctgcggccaa tccgctggcg gtcaaccgca acccgatcgg
    25801 tgatctgacg ctcgccggtg gcaagctgct gcgcaccagc tccggctcca tcgaactggc
    25861 ggccgggcgc gacatcgtgc tgggtggcac cacgacggcg ccggcgaccg tgatggtcac
    25921 cggcacggcc tcgacgctcg ctccggcgct cgcggccgag ttcaccacgc cggccaacgc
    25981 gctcttcacg gcccatggcg gccgactgac cgccagcgcc gggcgtgaca tccgcagtcc
    26041 ggtcagcgac cagaccctga tcaacaactg gttctggcgc tccggacggc tcgatgcagg
    26101 tggcaacttc tcatcgagca gcctggcctg gtggagccgc ctcgacctgt tccgtcacgg
    26161 cttcggctcc tttggcggcg gcaacatcct gctcagcgcc gggcggaacc ttcaggatgt
    26221 cggcgccttc gcccccagct cggcacgcat gacgtcgcgg atccccgatg catcgaagct
    26281 gctggtcgag aatggcggcg acatcgaggt tctggccggc ggcgacattg ccggcggcag
    26341 cttcttcgtc gggcggggtc aggggctgat cagggcggca ggcgaaatcg gcagcggcac
    26401 ggcactcaat tcgaccatcg gtcctctggc gccggtgctg gccctcaatg acggcggctg
    26461 gacgctgcgt ggccgcagcg acgtggcgat cactgccgcg ttcaacccga ccatcatggt
    26521 gcccaacagc agcaacagcg gcagcttctt ctacagctat ggcagcagtg ccactctgaa
    26581 tctggccagc acggccggcg actttcactg gcaagatctg agcagcgccg ggctcaacag
    26641 tctgcgggcg ctgcccaccg gcaccggcac cctgaatgaa gatctggtca gtttcgtgcc
    26701 ggtcaccact ccaaccccct acatcgcacc gccccgggta gagatcatgg cccatgccgc
    26761 ggatgtggtt ctgtccggca accgcaacaa cacgctgggg atctctcttt acccgtcggc
    26821 gctgggcagc ctggtgctgc atgccggtgg tgatctgttc ctcaacagct cgctgcggat
    26881 gatggacaac cggcctgccg acctggccgg tgtcaccaat ccggtcttca ccgccaccaa
    26941 cagcgccatt ctcggtctgg tgggcagcac catcggttac gacatcaaca agctgctgtt
    27001 caccgacctg catcaagcgg acagcaaccc ggtgcgcatc gatgctcgag gctcactcaa
    27061 tgtcgtggac ggcaagaagc tgatcatgcc caagcaggcg ctgctcagtg ccggacagga
    27121 catcctcaat ctgaccctgt tcggccagca ccatcgcagc actgacgtca ccctgatcga
    27181 ggccggtcgc aacctcctca acaccacctt gctcggcaac aatggtctga tctcgctggc
    27241 ggggcccggc aggctggaga tcagcgccgg acggcagctc aatctcggcg cctcggccgg
    27301 ggtggagacg gtgggcaacc tctacaacga cacgctgccg gtccagggcg cagcggtgca
    27361 gttggcggcg agccaggcgg ccacgctcga tgcgcagggg ctcgatcagt tcttgagtcg
    27421 ctatcttgcc gcctctggag ccccgagcgg cgcggattac cggaatgcac tggtcgatca
    27481 ggtcaggcag acgctcgggc agggcagaga ggtcgaatac ggctacgacg atgcactggc
    27541 gctgttccgt ggcttcggca gtgaggcgca ggtcgccttc atacagcagg ttctggcaca
    27601 ggagttcgcc aacagctatc tgcaaccggg ccagacctat gcggcagctt gggccgcgaa
    27661 tgccgctgcg gccggggtgg cggtggacca ccatgccggc agcgccttcg agcagatgcg
    27721 ccgcgaggtc atgttcaacg agatcaggct ggccggcatc gccggtgcgg ccgccaaggg
    27781 tgcggctgcc aaggaggcag gctatgcccc gggctaccgg gccatcgaat tggcgggcca
    27841 tggcgcaccc tttggcttcg ttggcggcat cgacctcagc cagagcaagg tgcagaccaa
    27901 gagcggtggc ggcatcgact tcgtcaaccc gggcggtgga gtgaacgtcg ggctgagtgc
    27961 cgaggtctcc aacgtcatcg gccaggccaa gtcggcggac cagcgtggcg tggtgacctt
    28021 tggcggtggc ggcatccgcg ccttcaccga tggcgatttt ctggtcaacg cacagaaggt
    28081 cttcgtgatc ggcagcggcg acatcctgct gtggagttcc aacggcaaca tcgactccgg
    28141 caagggctcg aacaacacgg tgacggtgcc gccactggtg ccgaaggtca atccgctcga
    28201 cggcagcatc tcgttcgagt tgccgaccct ggccaccggc agcggcatcg gcattctggc
    28261 cgcggccggc agcagcgcgg atggcgcggc ctacctgttt gcaccacgtg gcgaggtgat
    28321 tgcgctggat gccttcattc gcgcgccagt catcttcttc gatccggaaa agattcgtgg
    28381 cgccgacaac gcgctggggg tggcgcccaa tccggtcagc gcgccggcgg tgccgacagt
    28441 gaccggcctc ggcagcagcg ccacgcagaa cagtgaagcg cagagcaagg gcagcgaagc
    28501 gccccagcgc agcgaacgcg agagaagctc gattctgacg gtcgaggtgc tggcactcgg
    28561 cgagatgcct gccgctgccg gtggcgcgac aggcccggag ccggcgacgg agggcgatcc
    28621 cgacgagcgg aaaaagaaga aatcgataca gtgagtgatg ccggctgtgg cgacgaagcc
    28681 gatggcaggg agcgaccgaa gcgggcagga ctcgcgcctg ccgatgcggg ggttgttgaa
    28741 ccgaagccgc tgcggcatgc tttggcaccg ctgcgccgca gacaggagag ctcgatcgat
    28801 gccggaaaaa cctttttccc aagcctgcga aaacaaccgg cagccgatcg tcgagctgtt
    28861 gcagcgcatc ctggccgact gccgtgagct gctcgaaatc ggcagcggca ccggccagca
    28921 tgcggtccac tttgcgccgc ggctgcccca gctgcgctgg cagaccagtg acctggcgca
    28981 gcaccatgcc ggcatcagtg cctggcttgc cgagcagccg gcgcccaatc tgctgccacc
    29041 gatcgaactg gatgtcgacc ggttgccatg gccggtggcc ggcaaggatg gcattttcac
    29101 cgccaacacc tgccacatca tgggctggtc cacggtctgc aacctgtttc gtggtgcgca
    29161 ggcgctgctg ccgagcgggg gcctgctggt ggtctatggt cccttcaact atgggggcga
    29221 gttcaccgcg ccgagcaatg cccgtttcga tctctggctg aagcagcagg cgccgcagat
    29281 gggcattcgc gatttcgagg cggtggaccg actggcgaac gaggccggca tgacgctgcg
    29341 cgaagacaac gccatgccgg ccaacaaccg gctgctggtc tggcagaagc gttgaccttc
    29401 gcggttttca tgccctttgc cggctgtgct acagttagga tcgtgaattc gcgcacgaat
    29461 cctgtcgagg accgaggccg gcgtgccgat cgatgacctg ctgtttcaaa gccccatgga
    29521 ctgtcccggt tgagtgaccg atgaccagac cgaaccatct gtaccagctc attctcgtgc
    29581 tcacgctcgc gctctggagc gccgcggccg cggcgacacg tggtccggcg gcgctggagg
    29641 tcgatccgga gctgcgcagc catctgctga aggtgattcg cgacgaagag gcctcgctct
    29701 ttgtcgaccg cttcgatgcc gaagtgtgga tgatggacat ggagcagcgg ttgcgccgct
    29761 tcgtgccggc cacccaggag cggatcacgc tgctgcggct ggtgcaccgc gagtcggtgc
    29821 gtcatggcct gcggccggag attctgctgg cggtgatcga ggtcgagagt ctgttcgacc
    29881 cctatgcggt ctcttccagc ggtgcccagg ggttgatgca ggtgatgccg ttctggcgca
    29941 aggagatcgg ccgcccccat gacaacctga cccagaccga aatcaatgtc cgctacggcg
    30001 ccgcgattct ggcgcactac ctgcaacgcg agcgtggcaa cctgcccaac gcgctggccc
    30061 gctacaacgg cagcctgggg caggcgtggt atccgcgccg ggtgctgagt gcctggcgaa
    30121 agcactggta tctcggcgag atctgaacgc cgccagggtc agtcctgctc gttggctggc
    30181 cgccagcgat gcgccatctg ccgcgcggta tcgagcgcca gatggagtga cctcaagcgg
    30241 gtgagcagat gcagtgccat gtcgatgccg gccgcgacgc ccgccgaagt gacgaccttc
    30301 ccctcgtcca gatagcgcac cccctccacc acctgcaggt caccaaaacg ggcagccagt
    30361 tcggcgcaat cttcccagtg ggtggtgacg cggcggccgg cgagcaggcc ggcctgcgcg
    30421 gccagaaagg caccggtaca gaccgtggcg gtgatctgcg cgccttgggc agtggtggcg
    30481 atccagtcga tcagtgtcgt gcgatcgagc agggcgtcga tgtctccgcc gggcaggagc
    30541 agcaggtcga tcgcggggtg ctggtcaagg ccgtggctcg gcatcagcag cagatcgccg
    30601 cgactgcgga ccacgtggcc gggctgctcg gccacggtga tcagctcgaa cagcgccggt
    30661 gccgcagggt gggcacgccg gtgcagccgc gcggcggtcg agaacacctc gaacgggccg
    30721 gccagatcca tcggttcgat ctggtcgaac agcacaaagg ccactgtcat cggcgatgcg
    30781 gagggttgtt gccggatgac gaaatcatcg aagcgctgcg ttggcaatgg atcgacccgc
    30841 agccggtcga cccgggccag cgctgggccg cgccgcagcc actgtgcgag cgccgtgacc
    30901 gcctcttctt cgccgctcat caccacttcg acctgcccat ccgccaggtt gcgggcatgg
    30961 ccggtgacgc ccagtcgcag ggcttcgcgt cgggtggcgg cgcgaaagcc gacctgctgt
    31021 accttgccac tgacgagaac atgcaggcag cgtgtcatgc cgtactccga aggtcgcttt
    31081 gctctggcgg ataggttaac ttggggcccg ccggtccgta aaatgtgaac cagcgcacaa
    31141 catcgaccat tgcacggggt atggagatga ccgaaaagag cattgtgatc gaggccatcg
    31201 gaccacaggg cacacgctat caggagctgg ccaagggcat tcaggcgagc tttcgcggcc
    31261 gcggcatcgt ggtgcaggca cccaggacca agagcgaatc aagctggacc agcgtcatcg
    31321 tctcggtcag cagcggcatc gagaccgacc tgctcaacaa cctgatcgac ctgctgacga
    31381 cgctggccga tggcgagcag gagaagcatg acctgtcgat ccatctggaa ggtgacgagg
    31441 tcttctatga cctacagaaa gagaagcaga agttcctgac gcgttacccg cagatgaagc
    31501 cgaagtcagg ggccgtgacc aaggcgcggt cgaccgacga cctcgaagag tcggccatcg
    31561 agtagcggtc gacccttgca catttgagcc atcgaccctt cgtcagcgaa tggtcacctt
    31621 ctgtgcggcg attcgtgcct gccactgctc cggcccactg cggtgcaccg agttgcccga
    31681 ggtgtcgacc gcgactgtga ccggcatctc ttccacctcg aattcgtgga tcgcctccat
    31741 gcccaggtcg gcgaaggcca ccacccgtga ttttcgaatc gccttggaga ccagataggc
    31801 cgcaccgcca atcgcgatca ggtagaccgc cttgtgggcg cggatgctgt cgatggcagc
    31861 ctgcccccgt tcggccttgc cgaccatgcc gagcaggccg gtcttctcca gcatcacctc
    31921 ggtgaacttg tccatccggg ttgccgtggt cgggccggcc gggccgacca cttcgtcgcg
    31981 caccggatcg accggcccga cgtagtagat gaagcggccc ttgaagtcga caccgtccgg
    32041 caactgttcg ccgcgggcga agaggtcggc aatgcgcttg tgcgcggcat cgcggccagt
    32101 cagcatcttg ccgctcagca gcaggttgtc acccggcttc cagctggcga cctcgtcggc
    32161 ggtgagggtg tcgaggttga cgcgacggga cgtggggccg gcctcccaca ccagcttggg
    32221 ccagtcatcc agacgcggtg gcgtcagctc ggccggcccg gagccatcca gcgtgaagtg
    32281 gacatggcgg gtggcggcgc agttgggaat catcgccacc ggcagcgagg cggcgtgggt
    32341 ggggtggtcc atgatcttga cgtcgagcac ggtggtgagg ccgcccaggc cctgggcacc
    32401 gatgccgagg tcattgaccg cttcgaagat ctgcaaccgc agctcctcca gccggttgct
    32461 cgggccgcgg gctttcagtt catcgatgtc gatcggttcc atcagcgact ccttggccat
    32521 cagcatcgcc ttttcggcgg tgccgccaat gccgatgccc aacatgccgg gcggacacca
    32581 gccggcgccc atggtcggca ccgtcttcac cacccagtcg aggatgctgt cgctggggtt
    32641 gagcatcgcc agcttcgatt tgttctccga accgccaccc ttggcagcga cggtcacctc
    32701 gagtctgtcg cccggcacca gctccatgtg gatcaccgcc ggggtgttgt cacgggtgtt
    32761 gcggcgcgcg ccggccgggt cggccagcac cgagccgcgc agcaggttgt cggggtgggt
    32821 gtaggcgcgg cgcacccctt cgttgaccat ctcggtgacg ctgagcgtcc cctcccagcg
    32881 cagctgcatg ccgaccttca gaaagacgtt gacgatgccg gtgtcctggc agatcgggcg
    32941 gtggccctct gcgcagaggc gtgaattgac cagaatctgc gccatcgcct cctttgcggc
    33001 cggcgactcc tcggcctgat aggcgcggtg cagcgcctga atgaagtcga gtggatggta
    33061 gtaggagatg tactgaagcg cgtcggcgac gctgtcgatc agatcctgct ggcggatgag
    33121 ggtcatggga agggctcctg gtggacggtg tcgacgcgat gacgcgcatt ttactacagc
    33181 agcgcggtgg cactgctgca agccatgggt gcgggttcag atcgcctgca tccagtgttg
    33241 gtcggtgtga ctgtgggggt gcgtggcatc gacggcgatg cgcaccggct ccggatcgag
    33301 cgtgatcgtc accggcaggc agccatgcgg atcgccatcg agctggatcg gttcacccac
    33361 tgcgccttcg acgtggatgt ggctgccgag ggcccgccgc acgccgggca gtcggctgga
    33421 gcggccggtg ggcagcgcca gcagatagct cagcaggcca agccgggtgt ggcgctcgag
    33481 catgaccagg ttgaggtggg gcgcgaacag gctcgcctcg gggtcgagca cgaaggggcc
    33541 ggcatagtga cgggcatggc tgatgatcag cgagccgacc tgccactgct ggtcgttgct
    33601 gcgcacccga tagcgctggc ggccatagcg gccgaccatt ctggccatcg tcagcaggta
    33661 ggcacccttg cccagccggc gtttgaggtc gaggtcgacg ccattcacca cc
//
LOCUS       DAORCQ010000015        33457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_686_length_33457_cov_5.856476, whole genome shotgun sequence.
ACCESSION   DAORCQ010000015 DAORCQ010000000
VERSION     DAORCQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 33457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33457
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_686_length_33457_cov_5.856476"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PKI23_02625"
     CDS             <1..1341
                     /locus_tag="PKI23_02625"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNN35867.1"
                     /translation="TVQMHAIQTSGNCIRNVTSDQFAGIAPDEVVDPRPYCELIRQWS
                     TFHPEFAYLPRKFKIAVNGAKSDRAAIAVHDIGVQLDRNAQGEVVCQIWVGGGLGRTP
                     VIGTLIRDQLPERHLLGYLEAILRVYNMLGRRDNKYKARIKILVKALGAEEFTRLVEA
                     QWNGLRDGAITLTREEIERVQGFFTPPAYATLADRSAELAALAARQPAFGRWLEHNVM
                     PHKQPGYAAVTLSLKRTGIAPGDLDDWQMEAVADLADRFSFGELRVSHEQNLILADVR
                     QSELEQLWQQLEALHLATPNIGTLNDIICCPGGDFCSLANAKSIPIAEAIQRRFDDIE
                     QLYALGRIDLNISGCMNACGHHHVGHIGILGVDKKGAEYYQISLGGSAGEGEEAALGD
                     VMGPSFSQQQIPDVIEKIVKVYVEQRATGEKFIDTYRRVGLEPFKEQVYGPASH"
     gene            1322..1819
                     /locus_tag="PKI23_02630"
     CDS             1322..1819
                     /locus_tag="PKI23_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNN35868.1"
                     /translation="MAQPVIKHRALCQDQWQLLRDGDAVPAQGQLIVSLECWQTQRQA
                     LLERGSPLGIWLGCDQPPELIAADLPHFALIAIHFPTFTDGRGYSYARLLRDHYRYSG
                     ELRALGDIGLDQIYFLGRCGFDSLVLPESLDPDSALAAFDDFTAPYQGASDDPQPLFR
                     RHQRR"
     gene            2001..2618
                     /locus_tag="PKI23_02635"
     CDS             2001..2618
                     /locus_tag="PKI23_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNN35869.1"
                     /translation="MKLYSSMGPNPRLVRLFMAEKGLNIETVPVDLLAGENRKEPFLN
                     KNPAGQLPALELDDGTVISETVAICEYLEELQPQPALIGSNAQERAETRMWTRRIDLK
                     ICEPLGSGFRFAEGARLFESRMRIIPEAAPGLKAVAQDGLKWLDGQMAGKEFVCGKRL
                     TLADLMLFAFIDFGRGVGQNIDPGCTQLLAWYERMKQRPSAAATA"
     gene            complement(2667..4976)
                     /locus_tag="PKI23_02640"
     CDS             complement(2667..4976)
                     /locus_tag="PKI23_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35870.1"
                     /translation="MLRLLIQKSLAVVLLLPIGWFVYRFPLLRPELALFYGSYLLLLH
                     RYPRAWLLVVPALLPLLCLAPWSGRLFFDEFDALVLTTLAGILLVGQRAPVRVGWPPL
                     RVLLLLSLFLLLFIFGVLRGLWPPPSLDHNSTANYYSPLNSLRSGKGFLWALLLAWLW
                     RWQSGFDRASRLLFLRGTTLGGMGVLLVVLWERGVLENLFFYQNHWALLGSLLDFHTT
                     SRATALFFDMHVGGAAIDGYLLCALPLVAIHILHESRALPRAVAAMAFFGLLYVALVT
                     FSRGLYLGVLTLFVVAGLWIASHAGSRYPRIQFPLTFLCLLLLLLTSMLLQRHGGFLA
                     TCAALAAFSGAALLWGQSARLHWMVGLTLSGLILGSATALVVHAMLTSKWVSNTPMFA
                     WTMAAAATTIVVILGAVLMQRWKPYLSAPRQFSLLLAVSLTLALMVPSIFGYRMESRF
                     SSSLDDLSERIQHVRTSLGLMGNDVLTRLLGVGVGRFPASYYWQEEIANQKAVGTFWF
                     QHETGHENYLTFAGAHDLRLGQAIDMKPAERYLLSLDVRTADPEAALSLRICHRRLIE
                     PSEYNPLCWDRGEIVKGASGQWQHLQFTVDNEFLGSWRNQIKAPLFLLFSNRREYRFN
                     LIPQTFLDIDNLSLKDASGRELLSNGDFERGIDRWFGFYDFNHQHWHIESLLPHLYFE
                     LGGIGVGLFGLLVMVSIIRASRLVGAGELFHAAPMLSLCGVLSAGIFNGMVDAPRVQL
                     LIYLLLLLPLLQPGRFMVPARPEGQAQPS"
     gene            complement(5147..5743)
                     /locus_tag="PKI23_02645"
     CDS             complement(5147..5743)
                     /locus_tag="PKI23_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNN35871.1"
                     /translation="MAVALRKGQEHVVGKPPTAALPTLAFGLGWDVKQSFSKGVLGIG
                     AGKRKRAVDLDASCLLFDGNKELVDQVWFQQYQSEGGAVVHSGDNQSGSAPGADEIIH
                     VNLSKLPDKVQTLVFTVTCFSELSFDKVDNAFISLVDTVTRAEEARFDLAVEGGQHTA
                     LVIGKLTRRGNDWVFTALGVEGEGHTFVKLLPLIKTLI"
     gene            complement(5857..7533)
                     /locus_tag="PKI23_02650"
     CDS             complement(5857..7533)
                     /locus_tag="PKI23_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35872.1"
                     /translation="MTPSLDAEQQLPPSAGVTLSSSFALLYQGLHETLVESVKEARSL
                     VEESCHQPRSIATLSQLLHQIGGALRLAELKGGIELVDEIDAVVCAANSEGFDSQRAT
                     LLLDALDTLLRYLDCLHAGRKPLPALLLNAINRVRVASRRPPLPDSCFDLGHFRPSHP
                     LPEFGVAKTQPIDSQKRLRQLYQVGLTNLLREQNVEQSLGWMAHALDRLAPGGSNPWR
                     TTLWRLGNTLLRRFALDKRVPDDGGKRLFSALDRQIRLQIEQSDETALIETLRALITE
                     FVYLLRLDRSSDDAIDQWLTLIAAPELDHSERDLLVGRLHLDGEDQRTLDAVCGSLLN
                     ELQSLRRYLEGLAIAAPFRREVVSSFVDQFRELNGALHAGNLRVPVEEGLRLQQALLE
                     WLESGTDAPPTEAQLKQVAEQVVALEQRLIGFRHDDSAAGQQPPLLREARQALAQEAQ
                     ANLAICKQAIESYLSSRERLHLANLAEVLDSVRGAALFLERSELIELIERVLRFITLL
                     PARDGENLLAEVEALADILVILEFLFGTPVDQPLDAALLQLARVTAMTLS"
     gene            7662..8531
                     /gene="folD"
                     /locus_tag="PKI23_02655"
     CDS             7662..8531
                     /gene="folD"
                     /locus_tag="PKI23_02655"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNN35873.1"
                     /translation="MSAMLLDGKSLAQSCERQIAQRVTRLESASGGATPILATLLVGD
                     DPASATYVRMKGNACRRVGMKSHAIELPASTTTEQLLAEIDRLNRDPDVHGILLQHPV
                     PAQIDERRCFDAIAAEKDVDGVTCLGFGRMAMGEAAYGAATPAGIMRLLAHYQIPLIG
                     KHAVVVGRSPILGKPMALMLLNAHCTVTVCHSRTVDLADHLRRADLIVGAVGIPAFIR
                     ADWVKEGAVVVDAGYHPGGMGDVDLEGVKSRVMAYTPVPGGVGPMTINTLILQTLEAA
                     ERAQRQREIPPVE"
     gene            complement(8574..9476)
                     /locus_tag="PKI23_02660"
     CDS             complement(8574..9476)
                     /locus_tag="PKI23_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN35874.1"
                     /translation="MSNIRFDGRVAVVTGAGNGLGRSHARLLAARGAKVVVNDLGGDI
                     FGSGNDKRAADLVVDEIKAAGGEAVANYNSVEDGDRIIETAVNAFGRVDIVINNAGIL
                     RDKSFAKMTEEDWELIYRVHVLGAFRVLKAAWPIMREQAYGRIVNTVSAAGIYGNFGQ
                     VNYSMAKLGLHGMTQSLAQEGASKNIVVNSIAPIAGSRLTATVMPPQLLEALKPECVS
                     PLVAYLCSETNKDSGGLYEVGAGWIGKLRWERTQGVEFSSSGTFTPDDVASKWAELGD
                     FTNSTHPTNTQESMAPAMKLAGLG"
     gene            9659..10801
                     /gene="dapE"
                     /locus_tag="PKI23_02665"
     CDS             9659..10801
                     /gene="dapE"
                     /locus_tag="PKI23_02665"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNN35875.1"
                     /translation="MPDPIATLELAMELMRCPSVTPRDAGCQQLIGERLAQIGFRLEP
                     LPFGEVENLWARRGSTAPLLLFAGHTDVVPTGPVEAWSSPPFVPTLVDGILRGRGAAD
                     MKGSLSAMVTACEAFVAHHPQHRGSIAFLLTSDEEGPSVDGTVRVVETLMAREERIDF
                     ALVGEPSSDQRLGDVVKNGRRGSLNGWIKVIGKQGHIAYPQLADNPIQPLARALAELS
                     AIEWDRGNAFFPPTSFQISNISGGTGVTNVIPGTAEAVINFRYSTEVTAEQLEQQVEA
                     VLARHAHHFKIDWVRNGLPFLTGEGPFLDAVSAAVQTVTGQAPQLSTSGGTSDGRFIA
                     LTGAQVVELGPINATIHKVDEQVAVADLAQLAQIYFELLVRVLGTD"
     gene            10915..12318
                     /locus_tag="PKI23_02670"
     CDS             10915..12318
                     /locus_tag="PKI23_02670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN35876.1"
                     /translation="MAFLTFYGATRQVTGSCFLLEHAGQRLLLDCGMYQGGNETEAAN
                     REEFPFIPAEIDAVLLSHAHLDHCGLLPKLVKEGFRGPIYLTPATRDLLRLMLEDAAH
                     LENKDTEWENRQRLRSGDEPIEPLFDLQDVDSVLRQCRSIDYQRPEQILPGVTVTFRD
                     AGHILGSATVELQLASRDGRRRLLFSGDLGNSGSALLRDPEPIDGADLLLLESTYGDR
                     CHRTLDATLQELKEILVTAHRSGGNVLIPAFAVGRTQELLYWLGQFDRQGDLPQQQVF
                     LDSPMAISASEIYARHHHLFSPEEQRAFARTGSTEWESWLPKLRYSRTTADSMALNQI
                     QGGAIIIAGSGMCSGGRIRHHLKHGLWRRQNHLLIVGFQARGTLGRLLVDGAQRVRLF
                     GQEIAVNAKIHTVGGFSAHADQRQLIEWAGRLRDPAPKLFLVHGELEPMVTLQQAIHE
                     QLGWFAGIPVRGQKIEL"
     gene            12391..12846
                     /locus_tag="PKI23_02675"
     CDS             12391..12846
                     /locus_tag="PKI23_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNN35877.1"
                     /translation="MTFEPLYWHWLTLGLLLAGLEIFIPSFTVIWFGLGAILVGLLTW
                     LLPGLSITLQLLVWTGASITFAVGWFAFFKPRMVDHTRAGLSREAVLGEVGQVIELPL
                     DHRRGKVRFSKPLLGADEWAFICEEKVQCGDRVIVLDVSGNTLIVKRKT"
     gene            12875..13726
                     /locus_tag="PKI23_02680"
     CDS             12875..13726
                     /locus_tag="PKI23_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNN35878.1"
                     /translation="METTVVILALLALTLLTLFKGVRQVPQGSKWVVQRLGKYHSSLS
                     PGLNFIIPYIDSVAYKVTTKDIVLDIPSQEVITLDNVVIVANAVAYINILSPEKAVYG
                     VEDYQLAIRTLVQTSLRSIIGELTLDEALSSRDQIKTKLKTAISDDIADWGITLKTVE
                     IQDINPSQTMQKAMEEQAAAERLRRATVTRAEGDKTAAILQADGRLEASRRDAEAKVV
                     NATGTTEAIEKISSAIGQNQLPLIYLLGERYVDSLNDMATSNNAKLILLPADIPAAVR
                     GLMNAEK"
     gene            13713..14390
                     /locus_tag="PKI23_02685"
     CDS             13713..14390
                     /locus_tag="PKI23_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNN35879.1"
                     /translation="MPRSEVFAASRQPMPVRTLIFAKAPCAGIAKTRLIPALGAQGAA
                     RLAQRMLHHSVNEAIAARIGAVELCATPAIDDSAWAGQSLPRSISCSDQGAGDLGERM
                     ARAAQRTLHEGESLLLIGTDCPALDARRLQCAAQALQQVDCVITPTADGGYALLGLNR
                     FHPSLFAEIPWSSDRVAAITLQRLQQLKWSVQTQPTLHDIDHPADLRWLPAQWPSATE
                     LQADDMP"
     gene            14484..17012
                     /locus_tag="PKI23_02690"
     CDS             14484..17012
                     /locus_tag="PKI23_02690"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNN35880.1"
                     /translation="MDAINETELLSSTLDERTGLDKEALERAFFDHLFFSQGKFPALA
                     TMNDYYLALAYAVRDRLLHRWISTATAYTEQGARTVAYLSAEFLMGTQLGNNLLNLGI
                     HAQAQEAIEEMGLDFRELLRQEEEPGLGNGGLGRLAACFLDSLATLEIPSLGYGIRYE
                     FGIFDQEIVDGWQVEKTDKWLRFGNPWEIPRPEWAVEVKFGGHSEAYQDEQNRQRMRW
                     FPSWVVKAVPYDTPVLGYRNNTANTLRLWRAEAPESFDFAVFNRGDYYGAVDEKIRSE
                     NLSKILYPNDEAIQGKALRLQQQFFLVSASLQDMLRILRQQKIALEQFHEKFAVQLND
                     THPALAVCELMRLLLDDHRCTWEQAWSITRQSFAYTNHTLLPEALEQWPLALFQRILP
                     RHLELIYTINDQFLESVRLRYLGDVERLARLSIINEQGERYVRMAHLAAIGSHAINGV
                     AALHTELLKKDVLRDFHELWPEKISNKTNGITPRRWLFLSNPLLCRLISDALDPGWST
                     DLDRLRQLAPLADDAAFRQRWRSIKQHNKENLTRFIKKRFDTVINPESIFDVQVKRIH
                     EYKRQHLNALQIIAHYLRLKNSPNSEFFPRTYIFGGKAAPGYHMAKLMIKLINNVAAV
                     VNNDPDTRERMQVFFLPNFHVSNGERLYPAADLSEQISTAGKEASGTGNMKFMMNGAL
                     TIGTLDGANVEIREQVGEENFFLFGHTAEQIERLRASGYRPLDWLHGNDELQEVISLL
                     RSGYFSRGDSELFRPLLDSLLHHDPYFLLADFGAYMECQDQVNRVYLDHPEQWARMSI
                     FNTAHSGMFSSDRTIRQYCQEIWRVRPVPIRLLDPDDIHAEMMQ"
     gene            17070..17145
                     /locus_tag="PKI23_02695"
     tRNA            17070..17145
                     /locus_tag="PKI23_02695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17103..17105,aa:Arg,seq:cct)
     gene            complement(17255..18022)
                     /locus_tag="PKI23_02700"
     CDS             complement(17255..18022)
                     /locus_tag="PKI23_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNN35881.1"
                     /translation="MTEATRRLTGLLLALLCVLPLQAAPPDTIARIKPAILGLGTHQP
                     MGKPPSRLLGTAFVVADGRHAITNAHSLPEALQSDGVEKLVIFIGTAPQLKLREVRVI
                     EVDSDHDLALLGFDGEPLPALRIGDSQQVREGESYLFTGYPIGAVLGLYPVTHHAMVA
                     SITPVAVPQISATRISSRMVRELRDPYVVFQLDATAYPGNSGSPLYDPESGAVVGVIN
                     KVFVQQSKEAAIEHPSGITYAIPGQHIRELLRRNGLL"
     gene            complement(18015..19826)
                     /locus_tag="PKI23_02705"
     CDS             complement(18015..19826)
                     /locus_tag="PKI23_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNN35882.1"
                     /translation="MAGAEKSGLLGRVSFSGQSNAEADAPPLWPAWQRLSDAGVWLHW
                     HLPLLKHQDDAGTLLLGSAQFGSRLDGEALAQWWQSYQQQGDAALAQLEGHFLAIILD
                     RRRQCVTLAVDRFSTIPLYYAAAGDGVALASQLPLLRHAMAHRPALSMQGIYHYLYFH
                     MVPAPLSVWEGVHKIPAGHLLKISQGRAELVRYWQPVFLPAASGATQLRTQSAAAALL
                     VERLTTAVERAGAQSSAAFLSGGLDSSTVTGLLSRQREGDVDAYTVGFDAAGYDEMPY
                     ARIAARHFGVRHHELYLQPDDVVEIVPKIAGAYDEPFGNSSAVPTYFCAAAAAAAGHD
                     TLLAGDGGDELFAGNARYAKQKLFEHYLALPAWLRAGIEQGVERLPALGQVPLIQKGY
                     RYIEQARVPLPDRLESYNFFATHEPAQILHADLLAAIELDQPLKLLRDTYQAVTADHA
                     LQRMLWLDWKQTLADNDLRKVSVMTQSAGIQVRYPMLDDALIDLALQLPPDWLLPGQK
                     LRHFYKQAFSDFLPQQILTKPKHGFGLPFGVWMRDAPRLRELAYDALASLKKRTVLRS
                     DYLDQVVQLHQHDHAAYYGESIWIYMMLELWLQAWHD"
     gene            19964..21352
                     /locus_tag="PKI23_02710"
     CDS             19964..21352
                     /locus_tag="PKI23_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35883.1"
                     /translation="MIPALSRYIIYPLQERLLGRTTFSYLHELEQSQWLDRNQLEALQ
                     LRKLTQLLSTALRHSPWHAERIESAALADSIRNQSLTLSDLSRLPTMTKADASQQRER
                     IAWQQVPGGCHSYNTGGSSGQPLIFYFGRSRQASDAAGRMRARRWWGVEPGDREVYLW
                     GAPVELNKTDRIKTLRDRLINQWVLNAFRMSPADMRHYLVVLQQVQPRCIYGYASSLA
                     LLAAFCEAEGASLGLSELKVVCTTGEPLYPQQRALIQRVFGAPVANEFGSRDIGFTAH
                     ETPQGEMLLLSESMILEILDVEGRPVAADEIGEAVITGLCSEAQPFIRYRTGDMVRAA
                     DPAQCQDARGLHRIGEVIGRSTDFIVAADGTIMHALALIYVLRAVAGVGEFKIIQESL
                     DDCRVEVVPTAGWQAGSIDAIRNGLTARLGSGVRVDVRLVDEIPPEASGKHRYVVSRV
                     PLPAGLAGVLWH"
     gene            21395..22585
                     /locus_tag="PKI23_02715"
     CDS             21395..22585
                     /locus_tag="PKI23_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35884.1"
                     /translation="MLKKLLSVPVRYQLWRPLHWQLILADLFNRGGQPVRHHLTHLHG
                     TIDWLCRAQDVRVGHADSGGISAGWSFEDGWLPSYPETSGYIVETFLDAAALLGRQEL
                     VTRAQRILDWELSIQLDDGAFPGHFGEQGSHPVIFNTGQIMHGMNAGHERLGRTECLA
                     AAVRAGHWMLSKQDDDGCWRRSVHNGIPHTYNSRSAWALLKTGLLADDRALRDGAIRN
                     LDWVLTQQNEAGWFATNGFTPEGHAAPFTHTIAYAIRGLLESGLLLDDERYINAARRA
                     ACAVAAVLPVDGALPGTLDQQWQSDSYYVCLTGVAQMSMIWSRLIRCCDEQTLQPAVD
                     RALHYLKRHQRLKQDGRIDDGAIAGSSPLWGRYSMFEFPNWAGKFFADALMFDLAASA
                     RSKE"
     gene            22591..23415
                     /locus_tag="PKI23_02720"
     CDS             22591..23415
                     /locus_tag="PKI23_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HNN35885.1"
                     /translation="MSQLTTLILCGRSARHVHVANRLSAHSQVLAIVQENGSDLSLKK
                     VQQWLKPGVLRQKVSRWLRDRKRYRGNPEARFFFGDQPVRLDREDLRIEVPHINDPKV
                     VELANRLKPDLILVFGTSLIRGPLLEAGRLGMVNLHGGLSPEYRGADCTFWALYNREP
                     DKVGCTLHFINAGIDTGDLIAHICPPVTAQDDELTLFWRGVKASAEVYCELLDRLERG
                     EKPGVKQAGKGRLYQVKQRQGRHEAELAQAMRAGLLDGIDLPLRVRWFFKDEQVPG"
     gene            23463..24443
                     /locus_tag="PKI23_02725"
     CDS             23463..24443
                     /locus_tag="PKI23_02725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNN35886.1"
                     /translation="MSPFLISIVIPAHNRPQLLLEAINSVTAQNYSNYEVVVIDDGST
                     PPISHSALKDALGDRLTLHRHDSAQGVPKAKNAGINAAQGEVILLLDDDDLLMPNSLE
                     QIFHAFSKHPQIDCLFLGVRPFGAYHEGPEKSRKEAMKKILERTNPEKCDGLYFFSST
                     LFDALLDTVPIDFQRPAARRSMWNITGGFDENALFSESAWAIHAACIGTIALTEEAIT
                     QWRIHGNNFGWPSGLELEEIRLRQIENSISSSAQLMGKFVKKNQQWHERNRKIKKHHS
                     EQLFSKAYYLYGKNRKEGMKALAASSSLSFSKAHAKLWIKYFLSPLIARK"
     gene            24493..25296
                     /locus_tag="PKI23_02730"
     CDS             24493..25296
                     /locus_tag="PKI23_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35887.1"
                     /translation="MTNIIISYWVGRPPNRLYQLLNQIARHDAQAPFIITIVCNGGDR
                     HPLILPEKYVKNGVHVLNRVNSGYNIGAWEHGWRSDGESEYFLFLQDECRILKTGWLK
                     AFLDKMQESREIGLLGESINWKRAWSEQRNNPVAASCLNREGEVPFNAVDFLRAFIVL
                     SGIDPGESAEHLQSLILFSSRTVLEEVSGFITSDIYAEAVGSEIAISKKVQERGYRIA
                     QVGAQPFSFIGHLQWSVTWYRNWIKFKLFARPYKAKIEKVLEKISSRAD"
     gene            25299..25931
                     /locus_tag="PKI23_02735"
     CDS             25299..25931
                     /locus_tag="PKI23_02735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNN35888.1"
                     /translation="MKNYPHNPRQNMHAFIPTSARKVVDIGCNTGAFGEGLKNTRSIE
                     VWGVEPNANAAKLASQRLDNVIVSLFDADVPLPDATFDVVIFNDVLEHFAEPWNALRI
                     AARKLRPGGCVVASIPNLRQIDNLIHILKDKDFHYETMGIRDRTHLRFFTRKSILRLF
                     EESGYRVEILEGINEEWWTSSIWRRFGFRIFKDYLEDTKYIQFAVVAFPE"
     gene            26026..26865
                     /locus_tag="PKI23_02740"
     CDS             26026..26865
                     /locus_tag="PKI23_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNN35889.1"
                     /translation="MTPLLLLLGLAIGAVLGLTGAGGSIFAVPLLMWGLHWSLPQATP
                     VALLAVSASALIGTLAAWDVSYIRYRAALLMAGCGWFTAPLGIQLAHSLPVSLLNLLF
                     AVVLTIVALRMLRQAIRKPEEANVVRATVAGDGEPAAGPCCRLDPLTGRIQWTWRCAV
                     TIASGGGATGFLAGLIGVGGGFVIVPLLRATTELSMHSAVATSLMVIALTSAGTVASA
                     ILMQGIELPWGIALPFIAGTVAGMLGGRQLAPRIAGVRLQQLFALLMLIVAVGMAFHA
                     LSG"
     gene            complement(26878..27195)
                     /locus_tag="PKI23_02745"
     CDS             complement(26878..27195)
                     /locus_tag="PKI23_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35890.1"
                     /translation="MQILRQLIDTAALLAAHEDWRGLAEVDARLRNELDRVVKEVVER
                     ANPVELQALSEVVAELNGAYSHWLARGRDLRDQVSYEVRHYRRQLDGVEFYQRNGLPR
                     EEP"
     gene            complement(27227..27649)
                     /gene="fliS"
                     /locus_tag="PKI23_02750"
     CDS             complement(27227..27649)
                     /gene="fliS"
                     /locus_tag="PKI23_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNN35891.1"
                     /translation="MESHKMVYGNQAARQYRAVDTGSIVLEADPHRLIQMLFDGALER
                     LALAKGFLHNGEIAGRLQAVNGAIAIIDGLHGALDMERGGALASQLDQLYRYMNRRLV
                     EANLHRDASIIEEVAHLLRELKEGWDGIRPLALAAQPA"
     gene            complement(27663..29486)
                     /gene="fliD"
                     /locus_tag="PKI23_02755"
     CDS             complement(27663..29486)
                     /gene="fliD"
                     /locus_tag="PKI23_02755"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNN35892.1"
                     /translation="MPSVLGVGSGLDLPGVVRSLVAAEGNAKAAILATEQSAVELKIS
                     SYGLLASAVNDFKGAISAMRNLTAGGGISATSSDSKVVSATATAAAVPGNYEVIASQL
                     ATSHRLISLSETNGTTALGGGTLTLSNGSGKSFNVTIAAALSGLNDIRDAINNSPDNF
                     GVTASVVTTGTGSVLVLDGQGVGSDNAIKLTVADADTLNGDLSGLSRLAYDPLGSSGA
                     GKNMTEDRAAVDASIKVNGVTLTNKSGNLFDKAIDGVTLTLNSTSLTPVTVKVAAKSA
                     DRNAIEQALNKFVTGYNTLLGKLNPMISYDAVQKQGGALLGESSARLLSQQLRTLLVT
                     KVEGLPEGASSLSQIGLTSKRDGTLEINSTLLNSALNDHLDDTARLLASSGDPINQLH
                     KLESTPFTAVTDPVGSGTLTIHQGSKSFDVTVTAGVNDKVSDLISAINQAATSNGVAV
                     KASYRSLVGGGVALTLQSQSEGAAIRVTANDDDGNDSDAAGLSRLVFDPDGNALQMNQ
                     SQAASRSVAQGLVKSIDRMVDSYTGKSGIFSLKNSQLTESISRIEERRTALNRRLDQY
                     QQSLARQFAALDGLVGKLQSTQSYLTNQFDMLSNLAKGSSK"
     gene            complement(29576..29983)
                     /locus_tag="PKI23_02760"
     CDS             complement(29576..29983)
                     /locus_tag="PKI23_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNN35893.1"
                     /translation="MAIEPLLTRSLNSYSVKPQSPAASMVAADSVPEQSNQSIGGNSL
                     PARLAGVHLEADASPEQAQLAVQKLNEFIQKLQRDIHFDLDQQTGKTVITVRDRQTQE
                     VIRQIPPEYVIKMAKMLQDEISARQGQLVQTSA"
     gene            complement(30065..32284)
                     /locus_tag="PKI23_02765"
     CDS             complement(30065..32284)
                     /locus_tag="PKI23_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNN35894.1"
                     /translation="MALSVNTNLASLNTQRTLSISSGKLGTAMERLSSGLRINSAKDD
                     AAGLGISARMTSQIRGLDQAVRNANDGISLAQTAEGAMGEMSNILQRMRELSIQSANG
                     SNSATDRASLNGEVNQLKQELSRIANNTTFNGKRLLDGTLRSEQFQVGAQANETISMS
                     IGDGRATGLGNHAVNTSVNTSTTTNGIQANNGLEAASKRGFILSDGSVIGKVQTAGTT
                     NGYTATTFTTTAADGTTDTYLTTANDSAKTIAAGINSNLTGASATAYNMVTVAVSGTA
                     DATDADDITISDGTNTVTYSVAASGTATNAALVTAINADSAFTGAGGFARLNATSGNV
                     EIINTEGRDIRVALAKGTTTSGSTAVTVQGLGDSSATSVTNATTRTAGGQISLYLNQG
                     TSVAASATTIFTAAPTAVAVGLADASGGNGVAAQTLSVAGAQGTSSVAVNANDSAYTI
                     ATAVNQYSSTTGVSATARTVATLSGLSAAGTITFGLQGSNGTATTISANITGTGNLVD
                     LVTAINGKTGDTGITAKLNNSGGVELTQAEGHDIKITNFTHSAAVNNGSSSAPTQVAV
                     EQSITVTGGEGQSVKLYDGGNRDGADSTVVGGQVSFTGSAAYNVSSSIAGTVGSVFSG
                     AASSANASSLSSVNSVDISTTTGANDAIAVIDGAIDQINSIRSDLGAIQNRFLSAISN
                     MQATSENISAARSRVMDADFASETAALSKGQVMQQAGMAMLSQANALPQQVLSLLRG"
     gene            32659..>33457
                     /locus_tag="PKI23_02770"
     CDS             32659..>33457
                     /locus_tag="PKI23_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN35895.1"
                     /translation="MYPSVHAAKNPEKPAYIMASTGQVISYGELNERSIQVSRLFRSL
                     GLKVGDHVAILLENHPWFHIIGFGAHRAGLYFTPISSRLTASEVDYIVGDCTAGAFIT
                     QHAKSEVAEELTGLMPGVPAAARFMLDGSIAGFRSFEEAVLAQSTMPLEDETSGNEML
                     YSSGTTGRPKGILRPLSGEPITAPNRMTQTLPARYGFDENTLYLSPAPLYHAAPLGYN
                     RCVLCVGGTVIIMEKFDPEEALKLIEKYRITHSQWVPTMFVRMLKLPE"
BASE COUNT         6541 a        10405 c        10116 g         6395 t
ORIGIN      
        1 acggtgcaga tgcatgcgat ccagaccagc ggcaactgca tccgcaatgt caccagcgac
       61 cagttcgccg gcatcgcgcc cgacgaggtc gtcgatccgc gtccctactg tgagctgatc
      121 cggcagtggt ccaccttcca ccccgaattc gcctacctgc cgcgcaagtt caagattgcg
      181 gtcaatggcg cgaaatccga ccgcgccgcc atcgcggtgc atgacatcgg cgtgcagctc
      241 gatcgcaatg cgcagggcga ggtggtttgt cagatctggg tcggtggtgg ccttggccgc
      301 acgccggtca tcggcacgct gattcgcgat cagctgccgg aacggcatct gctgggctac
      361 ctcgaagcca tcctgcgggt ctacaacatg ctgggacggc gcgacaacaa gtacaaggcg
      421 cgcatcaaga ttctggtcaa ggcactcggc gccgaggagt tcacccgcct ggtcgaggcg
      481 cagtggaacg ggctgcgcga cggcgccatc acgctgacgc gcgaggagat cgagcgggtg
      541 cagggcttct tcactccgcc agcctatgca acgctggccg atcgcagtgc cgagctggcg
      601 gccctggcag ctcgccagcc ggctttcggc cgctggctgg agcacaatgt catgccgcac
      661 aagcagcccg gctatgcggc ggtgacgctg tcgcttaaac ggaccggcat tgcaccgggc
      721 gaccttgatg attggcagat ggaggcggtg gccgacctgg ccgaccgctt cagcttcggc
      781 gagctgcgcg tcagccacga acagaacctg attctcgccg acgtgcgcca gagcgaactc
      841 gaacagctct ggcagcagct cgaggcgctg catctggcca cccccaacat cggcacgctg
      901 aacgacatca tctgctgccc cggcggcgac ttctgctcgc tggccaacgc caagtcgatt
      961 ccgatcgccg aagcgatcca gcgccgtttc gacgacatcg agcagctcta tgcactgggc
     1021 cggatcgatc tgaacatctc cggctgcatg aatgcctgcg gccaccacca tgtcggtcac
     1081 atcggcattc tcggtgtcga caagaaaggg gcggagtact atcagatttc actcggcggc
     1141 tccgctggcg aaggcgaaga ggcggcgctc ggcgacgtga tgggcccctc tttctcgcag
     1201 cagcagatcc ctgacgtcat cgagaagatc gtcaaggtct atgtcgaaca gcgcgcgacg
     1261 ggtgagaagt tcatcgacac ctaccgccgc gtcggtcttg aacccttcaa ggagcaggtc
     1321 tatggcccag ccagtcatta agcatcgagc gctctgccag gatcagtggc aactgctgcg
     1381 tgatggcgat gccgtaccgg cacagggtca gctgatcgtt tcgctcgaat gctggcagac
     1441 gcaacggcag gcgctgctgg agcgtggttc gccactgggc atctggctcg gttgcgatca
     1501 gccgcccgag ctgattgccg ccgacctgcc ccacttcgcc ttgattgcga tccactttcc
     1561 gaccttcacc gatgggcgcg gctactccta tgcccgtctg ctgcgcgacc actaccgcta
     1621 ttccggcgaa ctgcgcgccc tgggtgatat cgggctggac cagatctatt ttctcggtcg
     1681 ttgcggcttt gactcactgg ttcttccaga gagcctcgat cccgacagtg cgctggccgc
     1741 ctttgatgac ttcactgccc cctatcaggg cgccagcgat gacccgcagc cgctgtttcg
     1801 ccgccatcaa cgaagatgac aactcaataa ctctataaaa cattgagttg agcgtttttt
     1861 ttgatgcatg agaagagctg ttttcggcgg tgatgcgacc atgaccccat ggccaccgca
     1921 gttgactgcc gacttgtggc cgaatgggct atcggcaaca atccacctgc cgagaatcac
     1981 aataacagga gaagatgaag atgaaactct actcgtcgat gggtcccaat ccgcggctgg
     2041 tccgactgtt catggccgaa aaagggctca acattgagac cgtgccggtc gatctgttgg
     2101 ccggagagaa ccgcaaggag ccctttttga acaagaatcc ggcaggtcaa ctgccggcac
     2161 tcgaactgga cgatggcact gtcatcagcg aaaccgtggc catctgcgaa tatctcgaag
     2221 agctgcaacc gcaaccggca ctgatcggca gcaacgccca ggagcgcgcc gaaacccgca
     2281 tgtggacccg ccgcatcgat ctgaagatct gtgaaccgct gggcagcggt tttcgctttg
     2341 ccgaaggggc cagattgttc gagtcgcgga tgcggatcat ccccgaagcg gcaccggggc
     2401 tgaaagccgt ggcccaggat ggactcaaat ggctggatgg ccagatggcc ggcaaggaat
     2461 tcgtctgcgg caagcggctg acgctggctg acctgatgct ctttgccttc atcgacttcg
     2521 gtcgtggcgt cggacagaac atcgatccgg gctgcaccca gttgctggcc tggtatgagc
     2581 gcatgaagca gcgccccagc gcggccgcca ctgcctgatg ccatcgggct gcccggtgag
     2641 tagcgatcac cgggcagcca ttcacctcat gacggttgcg cctgcccttc cggccgagcc
     2701 ggcaccatga agcgtccagg ttgcagcaac ggcaacagca gcagcagata gatcagtagt
     2761 tgtacccgcg gcgcgtcgac catgccgttg aagatcccgg cactcagcac tccgcacagc
     2821 gacagcatcg gtgcggcgtg gaacagctcc cctgccccga ccaacctgct ggcgcggatg
     2881 atcgagacca tcaccagcag gccgaacaga cccactccga tcccacccag ttcgaaatag
     2941 agatgcggca gcaggctctc gatgtgccaa tgctggtggt tgaagtcgta gaagccgaac
     3001 cagcggtcga tgccgcgctc gaaatcgcca ttgctgagca gttcccggcc cgatgcatcc
     3061 ttcagcgaga ggttgtcgat gtcgagaaag gtctgcggaa tcaaattgaa acggtattca
     3121 cgccggttgc tgaacagcag gaacagcggt gccttgatct gattgcgcca gctgccgaga
     3181 aactcgttgt cgaccgtgaa ctgcaggtgt tgccactgcc cggatgcgcc cttgacgatt
     3241 tcgcccctgt cccagcagag cgggttgtat tcggagggct cgatcagcct gcggtggcag
     3301 atgcgcagtg acagcgcagc ctcggggtcg gccgttctga catcgagact gagcagatag
     3361 cgctctgccg gcttcatgtc gatggcctga ccaagccgca gatcgtgggc cccggcaaag
     3421 gtcagatagt tttcgtgtcc cgtttcgtgt tgaaaccaga aggtgccgac ggccttctgg
     3481 ttggcaatct cctcctgcca gtagtaggag gctggaaacc gcccgacccc gacgcccagc
     3541 agtcgagtca gaacatcgtt gcccatcagg ccgagcgaag tgcgtacatg ctggatgcgt
     3601 tcagacagat catccagcga tgaagagaaa cggctctcca tccgatagcc gaagatcgac
     3661 ggtaccatca atgccagcgt caggctgact gcaagcagca gggagaattg ccgtggcgcc
     3721 gagagatatg gcttccagcg ctgcatcaga acagccccga gaatgaccac gatcgtcgtc
     3781 gctgccgctg ccatcgtcca ggcgaacatc ggcgtattgc tgacccattt gctggtcagc
     3841 atggcgtgga cgacgagtgc cgtggcactg cccagaatca ggccagacag cgtcagtccg
     3901 accatccagt gcaaacgggc cgattgtccc cacagcaggg ccgcacccga aaatgccgcc
     3961 agtgcggcac aggtcgccag aaagccacca tgacgctgca agagcatgct cgtcagcagc
     4021 aacagcagca ggcagagaaa ggtgagtgga aactggatgc gcggatagcg cgatccggca
     4081 tggctggcga tccagaggcc cgccaccacg aatagggtga gcactcccag atagagccca
     4141 cgcgagaagg tcaccagagc cacgtagagc agtccgaaga aggccatcgc cgccacggcc
     4201 ctcggcagcg ctcgcgattc atggagaatg tgaatcgcca ccagcggcaa ggcgcagagc
     4261 agatagccat cgatggctgc accgcccaca tgcatgtcga aaaacagcgc ggtggcgcga
     4321 ctggtggtat ggaaatcgag cagactgccc agcagtgccc agtgattctg gtagaaaaac
     4381 agattttcca gtacaccgcg ctcccacagt accaccagca gcacacccat tccgcccaag
     4441 gtcgtgccac gcagaaacag cagccgtgag gcgcggtcaa aaccgctctg ccagcgccac
     4501 agccacgcca gcagcaacgc ccacaagaag cccttgccgc tgcgcaggct gttgagcggg
     4561 ctgtagtaat tcgctgtact gttgtggtcc agcgatgggg ggggccaaag tccacgcagg
     4621 acaccgaaaa tgaagagcag cagaaacagg ctcagcagaa gcagaaccct cagcggtggc
     4681 cagccgaccc tgacgggcgc tctttgcccc acgagcagaa tcccggccag cgtggtcagc
     4741 accaaggcat cgaattcatc gaaaaagagc cggccactcc agggcgcaag gcagagcagc
     4801 ggcaacagcg cgggaaccac cagcagccag gcacgcggat atctgtgcag cagcagcaga
     4861 tagctgccat agaacagcgc aagttcagga cgcagcagtg gaaagcggta gacgaaccag
     4921 ccgatcggca gaagcagcac aacggccaga ctcttctgga tcagcaatct gagcatcggt
     4981 gaaggattct gcggtgccgt cagtggggcg ctcagttcga gggtttcaga accgaagctg
     5041 ctctgatcgg ctggatcaat ggatcggagg cgcctggtga gcccgcaaac ctgtgtcggc
     5101 agtgacaagg cggccccgcg ttgctgggcc gccttgtgcg actgcgtcag atcagggtct
     5161 tgatcagcgg cagcagcttg acgaaggtgt gtccctcgcc ctcgacaccg agggcggtga
     5221 agacccagtc attgccacgg cgggtcagct tgccgatcac cagtgcagtg tgttggccgc
     5281 cctctaccgc cagatcaaaa cgcgcctctt cggcacgggt gaccgtatcg accagtgaga
     5341 tgaaggcgtt gtcgaccttg tcgaaactga gttcgctgaa gcaggtgacc gtgaagacca
     5401 gcgtctgcac cttgtccggc agcttggaga ggttgacatg gatgatctcg tctgccccgg
     5461 gagcactgcc tgactggttg tcacccgaat ggacgaccgc gccgccttcg ctctggtact
     5521 gctggaacca gacctggtcg accaactcct tgttgccatc gaacagcagg caggaggcat
     5581 cgagatcgac cgcgcgcttt ctctttccgg caccaatgcc caagacgcct ttggagaagc
     5641 tctgtttgac atcccagcca agcccaaagg ccaaggttgg cagtgcggca gtgggtggct
     5701 tgccaaccac atgttcttgc ccttttctca gtgcaacggc catgaaagct cctcgcgatc
     5761 gttttattgt gatgaaaagc cgcagggtcg gtgcaatcaa ccctgcattt tctggcaagt
     5821 taactcagat cgatccggcg agcaattggc gagcagtcat gacagcgtca tggccgtcac
     5881 ccgggccagt tgcagcagtg cggcatccag tggttgatcg acgggtgtac cgaacaggaa
     5941 ctccagaatg accaggatgt cggccagggc ttcgacctca gcaagcagat tctctccatc
     6001 gcgcgccgga agcagggtga tgaaccgcaa cacccgctcg atcagttcga tcaattcgct
     6061 gcgttcgaga aagagcgcag cgcccctgac actgtcgagc acctcggcca gattggccag
     6121 atgcaggcgc tccctgctgc tcagatagct ctcgatggcc tgcttgcaga tcgccagatt
     6181 ggcctgggcc tcctgcgcca atgcctgcct cgcttcacgc agcagcgggg gctgctgacc
     6241 agcggcggaa tcgtcatggc gaaacccgat caaccgctgt tcgagcgcga cgacctgttc
     6301 agcgacctgc ttgagttgcg cctcggtggg cggcgcatcc gtccccgatt cgagccactc
     6361 cagcagcgcc tgttgcagtc gcagaccttc ttcaaccggg acgcggagat tgccggcatg
     6421 caatgcgcca ttcagctccc ggaactgatc aacgaaactg ctcaccacct cgcgccggaa
     6481 aggcgcagcg atcgccagcc cttcgaggta gcggcgcagg ctttgcagct cgttgagcag
     6541 cgaaccgcac acggcatcca gcgtgcgctg gtcttcgcca tcgagatgga gccgaccgac
     6601 cagcaggtca cgttcactgt ggtcgagctc gggggcggcg atcagcgtca accactggtc
     6661 gatcgcatcg tcactgctgc gatcgagccg cagcagatag acgaattcgg tgatcaatgc
     6721 acgcaatgtc tcgatgagcg ctgtctcgtc gctctgctcg atctgcagac ggatctgccg
     6781 gtccagtgcg ctgaacagcc gttttccccc gtcatccggc accctcttgt cgagagcgaa
     6841 gcgtcgcaaa agtgtattgc cgagtcgcca cagggtggtg cgccatggat tgctccctcc
     6901 tggcgccagt cggtcgaggg catgcgccat ccagccgaga ctctgttcga cattctgttc
     6961 ccgcaacagg ttggtcaacc cgacctgata gagttggcgc agccgtttct ggctgtcgat
     7021 cggctgggtt ttggcgacac caaattctgg caaggggtga ctcggtctaa agtgaccgag
     7081 atcgaagcat gaatccggca gcggcgggcg ccggctggcg acacgcaccc ggttgatcgc
     7141 attcagcagc agtgccggca gtggtttgcg ccctgcatgc aggcagtcca ggtagcgcag
     7201 cagggtatcg agggcatcga gcagcagtgt ggcccgttgt gaatcgaaac cttcgctgtt
     7261 ggcagcacag acaactgcat cgatttcatc caccagctcg atgccgccct tcagctccgc
     7321 caaccgcagt gcaccgccga tctggtgcag cagttggctc agggtggcga tcgagcgtgg
     7381 ctggtggcaa ctctcctcga ccagcgaccg cgcctccttc accgactcga ccaacgtctc
     7441 atgcagcccc tgataaagca gcgcaaaact ggatgagagc gtgacccctg cagagggtgg
     7501 cagctgctgt tcggcgtcca gactgggggt caagcgcgct ctccgtggat ggactgcgat
     7561 aaaatgacgc ctcatggcga tgcaatgcaa tagatatagc acaacccacc atggcgggtt
     7621 cttgaccact tcgacaaatt caacaacgga aatccaaatc gatgagtgcg atgctgctcg
     7681 atggcaaatc actggctcag agttgcgaac ggcagattgc ccagcgggtg acccggctcg
     7741 aatcggcgtc aggcggtgcc acgccgatcc tggccaccct gctggtcggt gatgacccgg
     7801 cctcggcaac ctatgtgcgc atgaagggca atgcctgtcg acgggtgggc atgaaatcac
     7861 atgccatcga actgccggca tcgaccacca ccgaacagct gctggccgag atcgatcggc
     7921 tcaaccgtga tcctgatgtc catggcattc tgttgcagca tccggtgccg gcgcagatcg
     7981 atgagcggcg ctgcttcgat gccattgctg ccgaaaagga tgtcgatggc gtcacctgtc
     8041 tcggttttgg tcgcatggcg atgggcgagg cggcttacgg cgcggccacg ccggcgggca
     8101 tcatgcggtt gctggcacac tatcagattc cattgatcgg caagcatgcg gtggtggttg
     8161 gtcgcagccc gatcctcggc aaaccgatgg cgctgatgct gctcaatgcc cattgcacgg
     8221 tgacggtctg ccactccaga accgtcgatc tcgccgacca tctgcggcgg gccgatctga
     8281 tcgtcggggc agtgggtatt ccggccttca ttcgtgccga ctgggtgaaa gagggtgccg
     8341 tggtggtcga tgcgggctac catccgggcg gcatgggtga tgtcgacctg gagggcgtca
     8401 agtcgcgggt gatggcctac acgccggtgc ccggtggcgt gggtccgatg acgatcaata
     8461 cgctgattct gcaaaccctg gaagcggcgg agcgggcaca gcggcaacgc gaaattccac
     8521 cggtagaatg aacgatcgcc ccgcacagtg gggcgatcgc gcagaccctg atctcagccc
     8581 agacccgcca gcttcatggc tggcgccatc gactcctggg tgttggtcgg atgagtcgag
     8641 ttggtgaaat cacccagttc ggcccacttg ctggccacat catccggggt gaaggtgccg
     8701 ctgctggaga attcgacccc ctgggtgcgc tcccagcgca gcttgccgat ccagccggcg
     8761 ccgacttcat agagcccacc gctgtccttg ttggtttcac tgcacagata ggccaccagc
     8821 gggctgacgc actcgggctt gagcgcttcg agcagttgcg gcggcatcac ggtcgcggtc
     8881 aggcgcgatc cggcgatcgg agcgatcgag ttgacgacga tgttcttcga tgcaccctcc
     8941 tgcgccaggc tctgggtcat gccatgcagg cccaatttgg ccatcgagta gttcacctgt
     9001 ccgaaattgc catagatgcc ggcggcggag acggtgttga cgatgcggcc ataggcctgc
     9061 tcacgcatga tcggccaggc ggccttcagc acgcggaagg cgccaagcac atggacgcga
     9121 tagatcagct cccaatcctc ttcggtcatc ttggcgaagc tcttgtcgcg cagaatgccg
     9181 gcgttgttga tgacgatgtc gacacgacca aaggcattga cggcggtttc gatgatcctg
     9241 tcgccatcct cgaccgagtt gtagttggcg acggcttcgc caccggcggc cttgatttca
     9301 tcgaccacca ggtcggcggc acgcttgtca ttgccgctgc cgaagatatc gccgcccaga
     9361 tcattgacca ccaccttggc accccgggcc gccagcagcc gtgcatggct gcgccccaga
     9421 ccattgccgg cgccagtgac cacggccacc cggccatcga aacgaatgtt actcatcctg
     9481 aattaccttc ttttgttacc tgttgcgcag aggccaacac agcgcatcgg cctccggtag
     9541 agaaaaagcc ttcatgacga aggcgcgcca ttatcccatg aatgcccgat ggcggcactt
     9601 caagggtccg gagattgggt atcatgcccg gcttcaaagc ttcaagagat cgacagcgat
     9661 gcccgacccg attgccaccc tcgaactggc catggaattg atgcgctgcc catcggtcac
     9721 gccgcgcgat gccggttgcc agcaactgat cggtgagcgg ttggcgcaga tcggctttcg
     9781 gctggaacca ctgccgtttg gcgaagtgga gaacctgtgg gcgcggcgtg gcagcactgc
     9841 accgctgctg ctgtttgccg gccacaccga tgtggtgcca accgggccgg tcgaagcctg
     9901 gagcagcccg cccttcgtgc cgactctggt cgatggcatc ctgcgcggcc gtggtgcagc
     9961 ggacatgaag ggcagtctgt ccgcgatggt gaccgcctgc gaagcctttg ttgcgcacca
    10021 tccgcagcac cgtggctcga ttgcctttct gctcaccagc gacgaagagg ggccatcggt
    10081 cgatggcacg gtgcgggtgg tcgaaaccct gatggcgcgt gaggaacgga tcgacttcgc
    10141 gctggtgggc gagccttcga gtgaccagcg tctgggtgac gtggtcaaga atggccgacg
    10201 cggttcactc aatggctgga tcaaggtgat cggcaagcag ggccacattg cctacccgca
    10261 attggccgac aatccgattc agccgctggc acgggccttg gccgagctga gcgcaatcga
    10321 gtgggaccgg ggcaatgcct tctttccgcc caccagcttt cagatctcca acatcagcgg
    10381 cggcaccggc gtcaccaacg tcatccccgg cacggccgag gcggtgatca attttcgcta
    10441 ctccaccgag gtgaccgctg aacagctcga acagcaggtc gaagcggtgc tggccaggca
    10501 tgctcaccac ttcaagatcg attgggtgcg caacggtctg ccattcctga ccggtgaggg
    10561 tccatttctc gatgccgtca gcgcggcagt gcagacggtg acgggtcagg cgccgcaact
    10621 ctccacctcg gggggcactt ccgatggccg tttcattgcg ctgaccggtg cccaggtggt
    10681 cgaactgggc ccgatcaacg ccaccatcca caaggtcgac gagcaggtgg cggtggctga
    10741 tctggcgcag ttggcgcaga tctacttcga gttgctggtg cgggtgctcg gcaccgactg
    10801 agtgccgggc cgcccggcag tgatgagggt gcgccgaaag ctggtagcat gaagccatgg
    10861 tgttccgccc atgacgacgg acggttttca cactcttcga ttccaaggtg tcccatggcc
    10921 tttttgacgt tttacggtgc cacacgccag gtcactggct cctgcttcct gctcgaacat
    10981 gccggccagc gcctgctgct cgattgcggg atgtatcagg gcggcaacga gaccgaagcg
    11041 gccaaccggg aggagttccc ctttatcccg gctgaaatcg atgccgtact gctctcccac
    11101 gcccacctcg accactgcgg cctgctgccg aagctggtca aggaggggtt cagaggcccg
    11161 atttacctga cgccggccac ccgtgatctg ctcagattga tgctcgagga tgcggcccat
    11221 ctcgagaaca aggacaccga gtgggaaaac cggcagcggc tgcgcagtgg cgacgagccg
    11281 atcgaaccac tgttcgacct gcaggatgtc gacagcgtgt tacggcagtg ccgttcgatc
    11341 gactaccaac ggcccgagca gattctgccc ggcgtgaccg tgacattccg cgatgccggc
    11401 cacattctcg gatcggccac cgtcgagttg cagctcgcca gccgtgacgg ccgcagacgc
    11461 ctgctgttct ccggcgacct cggcaacagc ggctcggcac tgctgcgcga cccggaaccg
    11521 atcgacgggg cggatctgct gctgctggag tcgacctatg gcgaccgctg tcatcgtacc
    11581 ctcgatgcca ccttgcagga gctgaaggag attctcgtca ctgcgcatcg atcgggcggc
    11641 aatgtcctga ttccggcctt cgccgtcggc cgcacccagg agctgctcta ctggcttggg
    11701 cagttcgacc gccaaggtga tctgccacag cagcaggtgt ttctcgacag tccgatggcg
    11761 atcagcgcca gcgaaatcta tgctcggcac caccacctgt tcagccccga agagcagcgc
    11821 gccttcgccc gcactggcag caccgagtgg gagtcatggc tgcccaagct gcgctactcc
    11881 cgcaccaccg ccgactcgat ggcgctgaat cagatccagg gcggagcgat catcatcgcc
    11941 ggcagcggca tgtgcagcgg gggccgcatc cgccaccacc tgaagcatgg cctgtggcgc
    12001 cggcagaacc atctgctgat cgtcggcttc caggcgcgcg gcaccctggg tcggctgctg
    12061 gtggatggcg cccagcgggt gcgcctgttc gggcaggaga tcgccgtcaa tgccaagatt
    12121 cacacggtcg gcggcttctc ggcccatgcc gaccagcggc aactgatcga atgggccggc
    12181 cggttgcgcg atccggcacc gaaactgttt ctggtgcatg gcgagctgga accgatggtc
    12241 acgctgcaac aggccatcca tgagcagctt ggctggtttg ccggcatccc cgttcgtgga
    12301 cagaaaatcg agctgtagcc accctcttct tgcggatgcc cggcgccgcg aaaacagccg
    12361 acacacgctg aaatggaacg agaaaccatg atgacctttg aaccgctcta ctggcactgg
    12421 ctgaccctgg gactgctgct ggcggggctg gagatcttca tccccagctt cacggtgatc
    12481 tggttcggtc tgggggcgat tctggtcggg ctgctgacct ggctgctgcc tggtctgtcg
    12541 atcacgctgc aactgctggt ctggactggc gcctcgatca cttttgcggt gggctggttt
    12601 gccttcttca aacccagaat ggtcgaccac acccgtgccg gcctgagccg cgaggcggtg
    12661 ctgggcgagg tgggccaggt gatcgagctg ccactcgatc atcgccgcgg caaggtccgc
    12721 ttcagcaaac cgctgctggg cgcggacgag tgggcattca tctgtgaaga aaaagtgcag
    12781 tgtggtgatc gtgtaatagt tctggatgtt tccggcaata cattgatcgt caaaagaaaa
    12841 acatgactca ttggccaaac aaagaggagc tcaaatggaa accaccgtcg tcatcctcgc
    12901 gctgctggcg ctgactctcc tgaccctgtt caagggtgtg cgtcaggtgc cgcagggtag
    12961 caaatgggtg gtgcagcgac ttggcaagta ccacagctcg ctgtcacccg gactcaactt
    13021 catcatccct tacatcgaca gcgtcgccta caaggtgacc accaaggaca tcgtgctcga
    13081 cattccgtcg caggaggtga tcacgctcga caacgtcgtg atcgtcgcca atgcggtggc
    13141 ttacatcaac atcctctcgc cagagaaggc ggtttatggg gtggaagatt accaactggc
    13201 cattcgcacc ctggtgcaga cctcgctgcg ctcgatcatc ggcgaattga cgctcgatga
    13261 agccctgtca tcgcgtgacc agatcaagac caaattgaag acggccatct ccgacgacat
    13321 cgccgactgg ggcatcacgc tcaagaccgt cgagattcag gacatcaacc catcgcagac
    13381 catgcagaag gcgatggagg agcaggccgc cgccgaacgg ctgcgccgtg ccaccgtcac
    13441 ccgcgccgaa ggcgacaaga ccgccgccat cctgcaggcc gatggccgcc tcgaagcctc
    13501 gcgccgcgac gccgaggcca aggtggtcaa tgccaccggc accaccgagg cgatcgaaaa
    13561 gatcagttcg gcgatcggcc agaatcaact gccactgatc tacctgctgg gagagcgcta
    13621 tgtcgactca ctcaacgaca tggcgacatc gaacaacgcc aaactgattc tgctgccggc
    13681 cgacatcccc gccgccgtgc gcggactgat gaatgccgag aagtgaagtc tttgccgcca
    13741 gcaggcagcc gatgccggta cggaccctca tcttcgccaa ggcaccctgc gccggcatcg
    13801 ccaagacccg gctgattccg gcgcttgggg cgcaaggggc cgcccgtctg gcgcaacgga
    13861 tgttgcatca cagcgtgaat gaggccatcg cagcacggat cggagcggtg gaactctgcg
    13921 ccacccccgc catcgatgac agcgcctggg cggggcaatc actgccgcgg tcgatctcct
    13981 gctctgacca gggagcgggt gatctgggcg agcgcatggc ccgtgccgcg cagcgcaccc
    14041 tgcacgaggg cgagtcgctg ctgctgatcg gcaccgactg tccggcgctg gatgcacggc
    14101 ggctgcaatg tgccgcacag gcactgcagc aggtcgactg cgtcatcacc cccaccgccg
    14161 atggcggcta tgcgctgctc ggactcaacc ggtttcaccc ctcgctgttt gccgaaatac
    14221 cctggagcag cgatcgagtg gccgccatca cgctgcaacg gttgcagcaa ctgaagtggt
    14281 cggtgcagac ccagccgacc ctgcatgaca tcgaccatcc agccgatttg cgctggctgc
    14341 cggcgcagtg gccgtcggcc actgaactgc aagcagacga catgccctga tccatcctgc
    14401 aagccggcaa tggcgtaccg ctgattcggc atcagacttg cttgttttat tgcatcactt
    14461 ttaccttttt tgcagcgggt gttatggacg ccatcaacga aactgaactg ctctcctcga
    14521 cactggatga acgcaccgga ctcgacaaag aggcgctcga gcgcgctttc tttgatcacc
    14581 ttttcttcag tcagggcaaa ttccccgcgc tggcaacgat gaacgactac tacctggcgc
    14641 tggcctatgc cgtgcgcgac cggctgctgc accgctggat cagcacagcc acggcctaca
    14701 ccgagcaggg tgccaggaca gtggcctatc tctcggcaga atttctgatg ggcactcaac
    14761 tcggcaacaa cctgctcaac ctcggcatcc atgcgcaggc gcaggaggcc atcgaggaaa
    14821 tggggctcga ttttcgcgaa ctgttgcgcc aggaagagga gcccggactc ggcaacggcg
    14881 gtctgggacg gctggcggcc tgctttctcg actcgctggc cacgctggag attccgagcc
    14941 tcggctacgg catccgctat gaattcggca tcttcgatca ggagatcgtc gatggctggc
    15001 aggtcgagaa gaccgacaaa tggttgcgct tcggcaaccc ctgggagatt cctcgccccg
    15061 agtgggcggt ggaggtgaag ttcggcggcc acagcgaagc ctatcaggat gagcagaacc
    15121 gccagcggat gcgctggttc cccagttggg tcgtcaaggc cgtgccctat gacacaccag
    15181 tgctgggtta ccgcaacaac accgccaaca ccttgcggct gtggcgggcc gaagcgccgg
    15241 aatcgttcga tttcgccgtg ttcaaccgcg gcgactacta cggcgcggtg gatgagaaga
    15301 tccgctcgga aaacctgagc aagatcctct accccaacga cgaagcgatt cagggcaagg
    15361 cgctgcggct tcagcagcaa ttctttctgg tctccgcctc actgcaggac atgctgcgca
    15421 tcctgcgcca gcagaagatt gcgctggagc agttccacga aaaattcgcc gtacaactga
    15481 acgacaccca tccggcactg gcggtctgcg aactgatgcg gttgctgctg gatgaccacc
    15541 gctgcacctg ggagcaggcc tggtccatca cccggcagag cttcgcctac accaaccaca
    15601 cgctgttgcc cgaagcgctg gagcagtggc cgcttgccct gttccagcgc atcctgccgc
    15661 gccatctcga gctcatctac acgatcaacg accaatttct ggagagcgtc cgcctgcgct
    15721 atctgggcga tgtcgaacgg ctggcccggc tgtcgatcat caatgagcag ggcgagcgct
    15781 atgtgcgcat ggcccatctt gcggccatcg gcagccatgc catcaacggc gtggcggcac
    15841 tgcacaccga actgctgaaa aaggatgtac tgagagattt ccacgaactc tggcccgaga
    15901 agatctccaa caagaccaat ggcatcaccc cgagacgctg gctgtttctg agcaatccgc
    15961 tgctctgtcg actgatcagc gacgcgctgg atcccggttg gagcaccgat ctcgatcgac
    16021 tcagacagct ggcgccactg gccgatgacg ccgcctttcg ccagcgctgg cgcagcatca
    16081 aacagcacaa caaggagaac ctgacccgct tcatcaagaa gcgcttcgac accgtgatca
    16141 atccagaatc gatcttcgat gtacaggtca agcgcattca cgaatacaag cgccaacatc
    16201 tcaacgccct gcagatcatt gcccactatc tgcggctcaa gaacagcccc aacagcgaat
    16261 tctttccgcg cacctatatc ttcggcggca aggcggcacc gggctaccac atggccaagc
    16321 tgatgatcaa actgatcaac aacgtcgccg ccgtggtcaa caacgacccc gacacgcggg
    16381 agcggatgca ggtgttcttt ctgcccaact ttcatgtcag caatggcgag cgcctctacc
    16441 cggcggccga cctctccgag cagatctcga cggctggcaa ggaggcgtcc ggcaccggca
    16501 acatgaagtt catgatgaat ggcgcactca ccatcggcac gctggatggc gccaatgtcg
    16561 agattcgtga gcaggtgggc gaggagaact tctttctgtt tggtcacacc gccgagcaga
    16621 tcgagcgact gcgcgccagc ggctaccggc cactcgactg gctccatggc aatgacgagt
    16681 tgcaggaggt gatctccctg ctgcgcagtg gctacttctc ccgcggagac agcgagctgt
    16741 tccgaccgct gctcgattcg ctgctgcacc acgaccccta cttcctgctg gccgatttcg
    16801 gcgcctacat ggagtgccag gatcaggtga accgggtcta tctcgaccat ccggagcagt
    16861 gggcacgaat gtcgattttc aacaccgccc actccggcat gttctcgtcg gatcgcacca
    16921 tccggcagta ctgccaggag atctggcggg tcaggccggt gccgatcagg ctactggacc
    16981 ccgatgacat ccacgccgaa atgatgcagt agccgctgaa ccaacgggct gctctacaag
    17041 cggcggctgg atcgctacaa tggcggcccg ccccggtggc acagaggata gcgcggcccc
    17101 ctcctaaggg gcaggtcaca ggttcgaatc ctgtccgggg cgccagacac gcctgcaaca
    17161 gagcagtcat gaaggccgca tcggctgatg cctgcctctt cggccgaccg aaacgcacgc
    17221 aacggcacgg cgccagagct gctgaaaacc gaccctaaag cagtccgttg cgccgcaaca
    17281 actcgcggat gtgctggccc ggaatcgcat aggtgatgcc gctggggtgc tcgatggccg
    17341 cctccttgct ctgctgtacg aacaccttgt tgatcacacc gaccaccgcg ccactctccg
    17401 gatcatagag cgggctgccg ctgttgccgg ggtaggcggt ggcatccagt tgaaagacca
    17461 catagggatc acgtagctcc cgcaccatgc ggctgctgat gcgggtggcc gagatctgcg
    17521 gcacggcgac cggggtgatg ctggccacca tggcgtggtg ggtgactgga tagagtccaa
    17581 ggacggcacc gatcggatag ccggtgaaga ggtagctctc cccttcacgc acctgctgcg
    17641 aatcgccgat gcgcaaggcc ggcaacggct cgccgtcaaa acccagcagc gccagatcat
    17701 ggtcactgtc gacctcgatc acgcgcactt cgcgcagctt caactgcggg gcggtgccga
    17761 tgaagatgac cagcttctcc acgccatcgc tctgcaacgc ctccggaagc gaatgggcat
    17821 tggtgatggc atggcggcca tcggcgacga caaaggcggt gccgagcagc cgcgacggcg
    17881 gcttgcccat cggctgatgg gtgccgaggc cgagaatcgc cggtttgatg cgcgcgatgg
    17941 tgtcgggcgg ggctgcctga agcggcagca cgcagagcag tgccaacagc agaccggtca
    18001 gccttcgcgt cgcttcagtc atgccaagcc tgcagccaga gttcgagcat catgtagatc
    18061 cagatcgact caccatagta ggcggcatgg tcatgctggt gcagctgcac cacttggtca
    18121 aggtagtcgg accgcagcac agtacgcttc ttcaggctgg ccagtgcgtc gtaggccagt
    18181 tcgcgcagtc gcggcgcatc gcgcatccag acaccaaacg gcaggccaaa gccatgcttg
    18241 ggcttggtga gaatctgctg tggcagaaag tcgctgaagg cctgtttgta gaagtggcgc
    18301 agtttctgtc ccggcagcag ccagtcgggc ggcagttgca gcgccaggtc gatcagggcg
    18361 tcatcaagca tcggatagcg cacctggatg ccggcactct gcgtcatcac gctcaccttg
    18421 cgcaggtcgt tgtcggccag cgtctgcttc cagtcgagcc agagcatgcg ctgcaacgca
    18481 tggtcggcag tgacggcctg ataggtatcg cgcagcagct tcagcggctg atcgagctcg
    18541 atggccgcca gcagatcggc atggagaatc tgtgccggtt cgtgggtggc aaagaagttg
    18601 tagctctcca gccggtcggg cagcggcacc cgcgcctgct cgatgtagcg gtagcccttc
    18661 tgaatcagcg gcacctgtcc gagtgccggc aggcgctcga cgccctgctc gatgccagcg
    18721 cgcaaccagg ccggcagcgc aaggtagtgt tcgaacagct tctgcttggc ataacgggca
    18781 ttgccggcaa aaagctcgtc gccgccatca ccagccagca gcgtgtcgtg ccctgccgcg
    18841 gccgcagccg cggcgcagaa gtaggtcggc actgccgagg agttgccgaa aggctcatcg
    18901 taggcgccgg cgatcttcgg cacgatctcg accacatcat ccggttgcag gtagagctca
    18961 tggtggcgaa cgccaaaatg gcgggcggcg atgcgggcat agggcatctc gtcataaccg
    19021 gctgcgtcga agccgaccgt gtaggcatcg acatcaccct cacgctgtcg gctcagcagc
    19081 ccggtcacgg tcgagctgtc gagtccgcca ctcaaaaagg cggccgaact ctgggcacca
    19141 gcccgctcga ccgccgtggt cagccgctcc accagcagtg ccgcagctgc cgattgagtg
    19201 cgcaactgcg ttgcaccgct cgccgctggc agaaagaccg gttgccaata gcgcaccagt
    19261 tcggcccggc cctggctgat cttgagcaga tggccggccg gaatcttgtg caccccttcc
    19321 cacacactca gcggcgcagg caccatgtgg aagtagaggt agtggtagat gccctgcatc
    19381 gacaatgccg gccggtgcgc catcgcatga cgcagcagcg gcaactgact ggccagtgcg
    19441 acgccatcgc ccgcggcggc gtagtagagc ggaatcgtgg aaaatcgatc gacggccaac
    19501 gtaacgcact gccggcgacg atcgagaatg atggccagaa aatggccttc gagctgcgcc
    19561 agcgccgcat caccctgttg ctggtaactc tgccaccact gcgcaagggc ctcgccatcg
    19621 aggcggctgc cgaactgcgc gctgccgagc agcagtgtgc cggcatcgtc ctgatgcttc
    19681 agcagtggca gatgccaatg cagccagact cccgcatcac tcagccgctg ccacgccggc
    19741 cagagtgggg gtgcatccgc ttcggcattg ctctggccac tgaaactcac ccgaccgagg
    19801 agtccgctct tttccgctcc cgccatgtcg actcctcgat gcaatgacta taatcaacga
    19861 attccggcat ggatctgccc tgcccctgcc acccttcgat gttgtcagac aggattgccc
    19921 agcacgtgac acttcaggca ctgacgccgg atagagcggc cgaatgattc ccgcactgtc
    19981 gcgttacatt atatatccgc tgcaggagcg gctgctggga cgcaccacct tcagctacct
    20041 gcatgaactg gaacagagtc agtggctcga ccgcaatcag ctcgaagctc tgcagctgcg
    20101 caagttgacg cagttgctgt cgacggcgct gcgccactct ccctggcatg ccgagcgcat
    20161 cgaatcggca gcgctggccg acagcatccg caaccagagt ctcacgctga gtgacctgag
    20221 ccgtctgccg accatgacca aggctgacgc atcccagcag cgggagcgga tcgcctggca
    20281 gcaggtgccg ggcggttgcc acagctacaa caccggcggt tcctccgggc agcccctgat
    20341 cttctacttc ggtcgcagcc gccaggcctc cgatgccgcc ggccggatgc gcgcccgtcg
    20401 ctggtggggg gtcgaaccgg gcgaccggga agtctatctg tggggcgcac cagtcgagct
    20461 gaacaagacc gaccgaatca agaccttgcg tgaccggctg atcaaccagt gggtgctcaa
    20521 tgcctttaga atgtcacctg ccgacatgcg gcactatctg gtcgtactgc agcaggtgca
    20581 gccgcgctgc atctatggct atgccagcag cctggcgctg ctggccgcct tttgcgaggc
    20641 cgagggggca tcgctcggat tgagcgaatt gaaagtggtc tgcaccaccg gtgaaccgct
    20701 ctatccccaa cagcgggcac tgatccagcg ggtatttggc gcgccggttg ccaatgagtt
    20761 cggcagccgc gacatcggct tcaccgccca cgaaacgccg cagggcgaga tgctgctgct
    20821 gagcgaaagc atgattctgg agattctcga tgtcgaaggc cggccggtcg cggccgatga
    20881 aatcggcgaa gcggtgatca ccggactctg ctccgaagcg cagcccttca tccgctatcg
    20941 caccggtgac atggtccgcg cagccgatcc ggcacagtgc caggatgcac gcggcctgca
    21001 ccggatcggc gaggtgatcg gccgctccac cgacttcatc gtcgcggccg atggcaccat
    21061 catgcacgca ctggcgctca tctacgtgtt gcgtgctgtg gccggtgtcg gcgagttcaa
    21121 gatcatccag gagagcctcg acgactgccg ggtcgaagtg gtacccaccg ccgggtggca
    21181 ggccggcagc atcgatgcga tccgcaacgg cctcaccgcc cggctcggca gcggtgtgcg
    21241 ggtcgatgtt cggctcgtcg acgagattcc cccggaagcc tccggcaagc accgctatgt
    21301 cgtcagccgg gtgcccctgc cagccggact tgcaggagtc ctttggcact gatcgtgcgt
    21361 catccattca tccctctcga tattcattga tcacatgctc aaaaaactgc tgtctgtccc
    21421 cgtccgctac caactctgga gaccactcca ctggcaactg attctggctg atctgttcaa
    21481 ccgtggcggc caaccggtcc ggcaccacct gacccatctg catgggacca tcgactggct
    21541 ctgccgcgcc caggatgtgc gtgtcggcca tgccgacagc ggtggcatca gcgccggctg
    21601 gagtttcgag gatggctggc tgcccagcta tcccgagacc agcggctaca tcgtcgagac
    21661 cttcctcgat gccgccgcac tgctcgggcg ccaggagctg gtcacccgcg cccagcgcat
    21721 actcgactgg gagctgtcga tccagctcga tgatggcgcc tttccgggcc actttggcga
    21781 acagggcagc catccggtca tcttcaacac cggccagatc atgcatggca tgaatgccgg
    21841 ccatgaacgt ctcggccgta ccgaatgtct ggctgctgcc gtgcgtgccg gccactggat
    21901 gctgtcgaaa caggacgacg acggctgctg gcgccgcagc gtgcacaacg gcattccgca
    21961 cacctacaac agccgctcgg cctgggcact gctgaagacc ggcctgctgg ccgatgatcg
    22021 cgcactgcgc gacggggcga tccgcaacct cgactgggtg ctgacgcagc agaacgaagc
    22081 gggctggttc gccaccaacg gcttcacgcc tgaaggtcat gccgcaccct tcacccacac
    22141 catcgcctat gcgatccgcg gcctgctcga aagcggcctg ctgctcgacg atgagcgcta
    22201 catcaacgcg gcgcgccgtg ctgcctgcgc ggtggctgcg gtgctgcccg ttgatggtgc
    22261 actgcccggc accctcgacc agcagtggca atcagacagc tattatgtct gcctgaccgg
    22321 ggtggcgcag atgagcatga tctggtcacg gctgattcgc tgctgcgatg aacagacgct
    22381 ccagcccgcc gtcgaccgcg cgctgcacta cctgaagcgc caccagcggc tcaagcagga
    22441 tggccgcatc gacgatggcg ccattgccgg ctcatcgccg ctgtggggac gctactcgat
    22501 gttcgagttt cccaactggg ccggcaagtt cttcgccgac gcactgatgt tcgacctcgc
    22561 ggcgtcggcc aggtcgaagg agtagtgaac atgtcgcaac tcaccaccct gattctctgt
    22621 gggcgctcgg cccgtcacgt ccatgtcgcc aaccggttgt cggcccactc acaggtgctg
    22681 gccatcgtgc aggagaacgg cagcgacctg agcctgaaaa aagtacaaca gtggctcaaa
    22741 cccggcgtgc tcaggcagaa agtcagccgc tggctgcgcg accgcaagcg ctaccgggga
    22801 aatccggagg ccagattttt cttcggcgat cagccggttc ggctcgaccg tgaagatctg
    22861 cgcatcgaag tgccccatat caatgacccc aaggtggtcg agctggccaa ccgtctcaaa
    22921 ccggacctga tcctggtttt tggcacctcg ctgatccgcg gtccgctgct cgaagccgga
    22981 cggctcggca tggtcaacct gcatggcggg ctctcgccgg aatatcgcgg tgccgactgc
    23041 accttctggg cgctctacaa ccgcgaacct gacaaagtgg gctgcacgtt gcacttcatc
    23101 aacgccggca tcgacaccgg tgacctgatt gcccacatct gcccgccagt caccgcgcag
    23161 gatgatgagc tgacgctgtt ctggcgcggc gtcaaggctt cggccgaggt ctattgcgaa
    23221 ctgctcgacc ggctggaacg cggtgaaaaa ccgggtgtga aacaagccgg caagggccgg
    23281 ctctatcagg tcaaacaacg gcagggccga catgaagccg aactggccca ggccatgcgt
    23341 gccggactgc tcgatggcat cgacctgccg ttgcgggtgc gctggttttt caaagacgaa
    23401 caggtgcccg gataaatgat cggcaggcac aatctctctt ctgggcccag gtgccatcca
    23461 ccatgtcgcc ttttctgatc tcgatcgtga tcccggcgca taaccggcct caacttctgc
    23521 tggaagcgat caacagcgtc actgcgcaaa attactcaaa ctacgaagtg gtcgtcatcg
    23581 atgacggctc aacgccgccg atttcacatt cggcattgaa agatgcactg ggagatcgcc
    23641 tcacgctgca ccggcacgat tcggcacaag gtgttcccaa ggcaaaaaat gccggaatca
    23701 atgcggcgca gggagaagtc atcctcctgc tcgatgatga tgacctgctg atgccaaact
    23761 cgctggagca gattttccac gctttttcca agcacccaca gattgactgc ctgtttctgg
    23821 gcgtccgtcc atttggtgca taccatgagg gaccagaaaa aagcagaaaa gaggccatga
    23881 agaagattct ggagagaacg aatccagaaa aatgcgatgg cttatatttt ttttccagca
    23941 ctcttttcga tgcccttctg gatacagttc caatagactt tcaacggccg gcggcacgcc
    24001 ggagcatgtg gaacatcacc ggaggattcg acgagaatgc tcttttcagc gaatcagcat
    24061 gggcgataca tgccgcctgc attggaacca ttgccctgac tgaagaggcc atcacgcagt
    24121 ggagaataca cggaaataat tttggatggc cctccggtct cgagctggag gaaataagac
    24181 tcaggcagat tgagaattca atttcatcct cagcgcaact catgggaaaa ttcgtaaaaa
    24241 aaaatcagca atggcatgag cgcaacagaa aaataaaaaa gcatcattcc gaacagctgt
    24301 tttccaaggc atattatctg tacggaaaaa accgaaagga aggcatgaag gcgctcgcgg
    24361 catcctcctc actttccttc agcaaggcac atgcaaaatt atggatcaaa tactttcttt
    24421 caccattgat cgcaagaaaa taaacaggcg ctgatttcac cgaaatactc cttctcggag
    24481 catgcgatca aagtgacaaa catcatcatt tcatactggg tcggccgccc accaaacagg
    24541 ctctaccagc tgttgaacca gatagccagg catgatgcgc aggctccatt catcataacc
    24601 atcgtttgca atggtggaga caggcacccg ctgatccttc ctgaaaaata tgtcaaaaac
    24661 ggcgtccatg tgctcaatcg ggtaaactcc ggatacaaca tcggcgcatg ggagcatgga
    24721 tggcgttccg atggtgaaag tgaatatttc ctttttcttc aggatgagtg tcgcattctg
    24781 aaaacaggct ggctgaaggc ctttctggat aaaatgcagg agtccagaga aattggactg
    24841 ctgggtgaaa gcatcaactg gaaacgtgca tggagcgagc aaaggaacaa tccggtcgct
    24901 gcaagctgcc tcaacaggga aggcgaagtg ccgttcaatg ccgtcgattt tctgagagct
    24961 tttatcgtcc tgagcggcat cgaccctgga gaaagcgcgg aacatctgca aagtctgatt
    25021 ctgttttctt ccagaacagt ccttgaagag gtttccggat tcataaccag tgacatctat
    25081 gctgaagcgg taggaagtga aatcgccatc tcgaagaagg tccaggagcg cggatatcgg
    25141 attgctcaag tcggagcaca gccattttcc tttatcggtc accttcaatg gagcgtcaca
    25201 tggtacagga actggataaa attcaagctg ttcgcaaggc catacaaggc taaaattgaa
    25261 aaagtcttag aaaagatttc cagtcgagca gattgaaaat gaaaaattac ccccacaacc
    25321 cacgtcagaa catgcatgcc ttcattccaa cttcggcacg caaagtggtt gacatcggtt
    25381 gcaacaccgg cgcctttgga gaagggttga agaacacgcg ctccatcgag gtttggggtg
    25441 tcgaacccaa cgccaatgca gcgaaattgg caagtcagcg actggacaac gtcattgttt
    25501 cactcttcga cgcagatgtt cccctgcctg atgcaacctt cgatgtggtg attttcaacg
    25561 atgttctgga gcatttcgca gaaccgtgga atgcattgag aatcgctgcc agaaaattgc
    25621 ggccaggagg atgcgtcgtg gcttcgattc cgaacctcag gcaaatcgac aacctcattc
    25681 atatcctcaa ggataaagac ttccattacg aaaccatggg cattcgcgat cgtacacacc
    25741 ttcgcttttt tacccgaaag agcatccttc gtctatttga agagagtggc tatcgtgtcg
    25801 agattctcga aggaatcaat gaggagtggt ggacctcttc catttggaga cgttttggtt
    25861 ttcgcatctt caaagattat ctcgaagaca ccaaatacat ccagtttgca gtggtcgctt
    25921 ttccagaatg atccaatcaa gacaagcaag aaaccgcgac tcctcgaccc cactcaaatt
    25981 tctgctgagc tctacaccaa aacgtgacgg atgaatgaat agcgcatgac cccccttctg
    26041 ctcttgctgg gtctggcgat tggtgcggtg ctggggttga ccggtgctgg cggctccatc
    26101 tttgccgtac cgctgttgat gtggggcttg cactggagtc tgccgcaggc aacgccggtt
    26161 gcactgcttg ccgtcagtgc ttcggcactc atcggcactc tcgccgcctg ggatgtcagt
    26221 tacatccgct accgcgccgc gctgctgatg gccggttgcg gctggttcac ggcgcctttg
    26281 ggcatccagc tcgcgcattc actgccggtc tcactgctta acctgctgtt tgccgtggtg
    26341 ctgaccatcg ttgcgctgcg gatgctgcgc caggccatcc gcaagcctga agaggccaat
    26401 gtggtacgcg ccacggtggc aggtgatggt gaaccagcgg ctggcccctg ttgccggctc
    26461 gacccgctca ctgggcgcat tcagtggacc tggcgctgcg cagtgaccat cgccagtggt
    26521 ggtggcgcca ctggctttct ggccggtctg atcggggtcg gtggcggctt cgtgatcgtg
    26581 cctctgctgc gggccaccac cgagttgtcg atgcactcgg ccgtcgccac ctcactgatg
    26641 gtcattgcac tgaccagcgc cggcacggtg gccagcgcca tcctgatgca gggcatcgag
    26701 ttgccctggg gcattgcgct gcccttcatt gccggcacgg tggccggcat gctgggagga
    26761 cgccagctgg cgccacgcat tgccggggta cgccttcagc aactgtttgc gctgttgatg
    26821 ctgatcgtcg ccgtcggcat ggccttccat gccctcagcg gctgagtcga tgcaccgtca
    26881 gggctcttct ctgggcagtc cgttgcgctg atagaactcc acgccgtcga gttggcggcg
    26941 atagtggcgc acttcgtaac tgacctgatc acgcagatca cgtccacgtg ccagccagtg
    27001 gctgtaggcg ccattgagtt cggcgaccac ttcactcagc gcctgcaact cgacagggtt
    27061 ggcgcgctcg accacctcct tcaccacccg atcgagctcg ttgcgcaatc tggcatcgac
    27121 ttcggccaat cctcgccaat cttcatgtgc tgccaacagc gcagcagtat caatgagttg
    27181 tctcaaaatc tgcatgaatt tcatccagcc cggaattcgc caagtatcag gccggctgcg
    27241 ctgccagcgc aagggggcga atgccgtccc agccctcttt cagctcacgc agcagatgtg
    27301 ccacctcttc gatgatgctg gcatcgcggt gcagattggc ctcgaccaga cgacggttca
    27361 tgtaacgata gagttggtcg agctggctgg ccagcgcgcc accgcgctcc atgtcgagtg
    27421 caccatgcag gccatcgatg atggcgatgg cgccgttgac cgcctgcagc cgtccggcaa
    27481 tttcgccgtt gtgcagaaag cccttggcca gcgccaaccg ctccagtgcg ccgtcgaaca
    27541 gcatctggat cagccggtgc ggatcagcct caagcacgat gcttccggtg tcgaccgccc
    27601 gatattgccg tgccgcctgg ttgccgtaga ccattttgtg tgactccatg agttttggtt
    27661 gtttatttgc tgctgccctt ggccagattc gacagcatgt cgaactggtt ggtcaggtag
    27721 ctctgtgtcg attgcagctt gccgaccagg ccatcgaggg ccgcgaactg gcgcgccaga
    27781 ctctgctgat actgatcgag ccgtcggttc agcgcggtgc gccgctcttc gatcctgctg
    27841 atgctctcgg tcagctggct gttcttcagc gagaagatgc cgctcttgcc ggtgtagctg
    27901 tcgaccatcc ggtcgatcga tttcaccaac ccctgcgcca ccgaacggct ggccgcctgg
    27961 ctctgattca tttgcagggc gttgccatca gggtcgaaca ccagccgcga cagcccggcc
    28021 gcatcactgt cgttgccatc gtcatcgttc gccgtcaccc gaatcgccgc gccttcgctc
    28081 tggctttgca gcgtcagcgc aacgccgccc cccaccagcg aacggtacga ggccttcacc
    28141 gccacaccgt tgctggtcgc ggcctgattg atggcggaga tcagatccga caccttgtcg
    28201 ttgacgccgg cggtgaccgt gacatcgaag cttttgctgc cctggtggat ggtcagggtg
    28261 cccgagccca ccggatcggt gaccgccgtg aagggcgtcg attccagttt gtgcagctgg
    28321 ttgatcggat cgccgctcga cgccagcagg cgtgccgtgt catcgaggtg gtcattcagg
    28381 gcactgttga gcagggtgga gttgatctcc agcgtgccgt cgcgcttgct ggtgaggccg
    28441 atctgcgaca ggctgctggc gccctcgggc agcccctcga ccttggtgac cagcagcgtg
    28501 cgcaactgct gactcagcag ccgcgccgag ctctctccca gcagcgcccc gccctgcttc
    28561 tggacggcgt catagctgat catcggattg agcttgccca gcaacgtgtt gtagccggtc
    28621 acgaacttgt tgagcgcctg ctcgatggcg ttgcggtcgg cacttttggc cgccaccttg
    28681 accgtcaccg gcgtcagcga tgtgctgttg agggtcagcg tcacgccgtc gatcgccttg
    28741 tcgaacaggt tgccagactt gttggtgagg gtgacgccat tgaccttgat cgaggcatcg
    28801 accgcggcac ggtcttcggt catgttcttt ccagcgcccg agctgcccag cggatcatag
    28861 gccaaccgcg acagtccgct cagatcgcca ttgagggtgt cagcatcggc cactgtcagt
    28921 ttgatggcgt tgtcgctgcc gactccctgg ccatcgagca ccagcacgct gccggtgccg
    28981 gtggtgacca ccgaggcggt gacgccgaag ttgtccgggc tgttgttgat ggcgtcacgg
    29041 atgtcattga ggccactcag tgccgccgca atggtgacat tgaagctctt gccgctgcca
    29101 ttgctgaggg tcagggtgcc gccacccaat gcagtggtgc cattggtttc gctcaggctg
    29161 atcagccgat ggctggtcgc cagttggctg gcgatcacct cgtaattgcc cggcaccgcc
    29221 gcagcggtgg ccgtggcact gaccaccttg ctgtcgctcg aggtcgcgct gatgccgcca
    29281 cctgccgtga ggttgcgcat cgcactgatc gcgcccttga aatcgttgac ggccgacgcc
    29341 agcagaccat aggaggagat cttcagctcg accgcgctct gctcggtggc cagaatcgct
    29401 gccttggcat tgccttcggc cgccaccagc gatctgacca cacctggcaa atccagtccg
    29461 gaaccgacgc caagaaccga tggcattgag tcatcctctt caagtgtttg tctcacccgc
    29521 cagcacggcg cgccagccct ttcagcggcg ctcagttcgc gcgccctgtt tcagtctagg
    29581 ccgaagtttg caccagctgg ccctggcggg cgctgatctc gtcttgcagc atctttgcca
    29641 tcttgatgac gtactcgggc ggaatctgcc ggatcacctc ctgtgtctgg cgatcgcgca
    29701 ccgtgatcac ggttttacca gtctgctgat cgagatcgaa atggatgtcg cgctgcagtt
    29761 tctggatgaa ttcgttgagt ttctgcacgg ccaactgcgc ctgttcgggt gaggcatcgg
    29821 cttcgaggtg aacacctgcc agccgcgccg gcaaactgtt gccgccaata ctctgattgg
    29881 attgctccgg cacggagtcg gcagccacca tcgaggcagc cggtgactgc ggtttgactg
    29941 aatagctatt gagggaacgg gtcagtaatg gttcgattgc catggccgca cctgtgtact
    30001 gctcatgaag tgttcaaaca gaaacaggag gccggcaccc cggcctcctg tccccttcat
    30061 gccatcaacc tctcagcagt gacagcacct gctgcggcag cgcattggct tgcgacagca
    30121 tcgccatgcc cgcctgctgc atcacctgcc ccttgctgag ggcagccgtt tcgctggcga
    30181 aatcggcatc catgacccgt gagcgggcag cgctgatgtt ctccgacgtg gcctgcatgt
    30241 tggagatcgc cgacaggaag cggttctgaa tcgcacccag atcagagcgg atgctgttga
    30301 tctggtcgat ggcgccatcg atcaccgcaa tcgcatcatt ggcgccggtg gtggtgctga
    30361 tgtcgacgct gttcactgat gacaggctcg atgcattggc cgaactggca gcgccagaga
    30421 agaccgagcc aaccgtaccg gcaatgctcg acgagacgtt gtaggctgcc gagccggtga
    30481 agctcacctg gccgcccacc accgtcgaat cggcaccatc acggttgcca ccgtcataga
    30541 gtttgaccga ctgcccttcg cctccggtga cggtgatcga ctgctcgaca gccacctggg
    30601 tcggtgcgct cgacgagcca ttgttcaccg ccgcactgtg ggtgaagttg gtgatcttga
    30661 tgtcatggcc ttcggcctgg gtcagctcga cgccgccact gttgttgagc ttggcggtga
    30721 tgccggtgtc accggtcttg ccgttgatgg cggtgaccag atcgaccagg ttgccggtgc
    30781 cagtgatgtt ggcgctgatg gtggtcgccg tgccattgct gccctgcagg ccaaaggtga
    30841 tggtgccggc cgccgacagc ccggagaggg tcgccaccgt gcgtgccgtg gcgctgacgc
    30901 cggtggtgga gctgtactgg ttgaccgccg tagcgatggt gtaagccgaa tcattggcat
    30961 tgacggccac cgatgaggtg ccctgcgcgc cagccacact caaggtctga gcggccacgc
    31021 cattgccacc gctggcatcg gccagcccca cggccaccgc ggtcggagca gcggtgaaga
    31081 tcgtggtggc cgaggccgcc accgaagtac cctgattgag atagaggctg atctggccac
    31141 cggcggtgcg ggtggtggcg ttggtaacgc tggtcgcact gctgtcgccc agaccctgca
    31201 cggtgaccgc ggtcgaaccg gaagtcgtgg tgcccttggc cagagcgacc cggatgtcgc
    31261 gcccttcggt gttgatgatc tcgacattgc ccgaggtggc gttgaggcgg gcaaagccgc
    31321 cggcaccggt gaaggcgctg tcagcattga tcgccgtcac cagtgccgcg ttggtggcgg
    31381 taccgctggc ggcgaccgaa taggtcacgg tgttggtgcc atcgctgatg gtaatgtcat
    31441 cggcatcggt ggcatcggcc gttccgctga ccgccaccgt gaccatgttg taggcggtgg
    31501 ccgaggcgcc ggtcaggttg ctgttgatgc cggcagcgat ggtcttggcg ctgtcgttgg
    31561 cagtggtgag gtaggtgtcg gtcgtgccat cggctgccgt cgtggtgaag gtggtggcgg
    31621 tgtagccgtt ggtggtgcct gccgtctgca ccttgccgat caccgagccg tcggagagga
    31681 tgaaaccgcg cttgctggcg gcttcaaggc cattgttggc ctgaatgccg ttggtggtgg
    31741 tgctggtgtt gacactggta ttgacggcat ggttgccaag gccggtggcg cggccatcgc
    31801 cgatcgacat gctgatggtt tcgttggcct gtgcgccaac ctggaactgc tcgctgcgca
    31861 gcgtgccatc cagcagccgc ttgccgttga aagtggtgtt gttggcgatc cgtgacagct
    31921 cctgcttgag ctggttgacc tcgccattga gcgaggcgcg gtcggtggcg ctgttgctgc
    31981 cgttggccga ctggatcgac agttcacgca tccgctgcag gatgttgctc atctcgccca
    32041 tcgcgccttc ggcggtctgc gccagcgaga tgccgtcgtt ggcattgcgc acggcctgat
    32101 ccagaccgcg aatctgcgag gtcatccgcg ccgagatacc cagaccggcc gcgtcatcct
    32161 tggcgctgtt gatgcgcagg cccgacgaga gccgctccat cgccgtgccc agtttgcccg
    32221 atgagatcga cagcgtgcgc tgggtattga gcgacgccag gttggtgttg acactgagag
    32281 ccatttttca ttccccttaa ggagtcaccg cattgatgcg gctttgcttg cattagcgga
    32341 tagatcgagg attcgacatt caccgctccc aagagagtta tcggccagtg gtgacggaga
    32401 tttagccttg cgggggattt ttttttcaca agctccaccg tcgacgcggt ggcgtgattg
    32461 atccggctac cggccagcgc ggacgccatt gccaaatcga ggtcgcttgg cctattctgc
    32521 ccggctgccg caaaccgtcc gtccgagacg gtatcggcca atacccgaaa gattcaataa
    32581 cagcctggca gccgcgtgac tctgcgcaga cgcagcggca ctgccagtac tctaatgatc
    32641 cgactggagc agttgcccat gtatccgtcc gtccatgccg ccaagaaccc cgaaaaaccc
    32701 gcctacatca tggccagcac cggccaggtg atcagctacg gcgagctgaa tgagcgttcg
    32761 atccaggtgt cacggctgtt ccgcagcctg gggctgaagg tgggcgacca tgtggcgatc
    32821 ctgctcgaaa accacccctg gtttcacatc atcggctttg gcgcgcatcg cgccgggctc
    32881 tacttcaccc cgatcagctc gcggctgacc gccagcgagg tcgactacat cgtcggcgac
    32941 tgcaccgccg gtgccttcat cacccagcat gccaagagcg aagtggccga ggagctgacc
    33001 ggcctgatgc cgggggtgcc ggctgcagcg cgcttcatgc tcgatggcag catcgccggc
    33061 ttccgctcgt tcgaagaggc ggtgctggcg cagtcgacca tgccgctgga ggatgagacc
    33121 tccggcaacg agatgctcta ctcctccggc accaccgggc ggccgaaggg cattctgcgg
    33181 ccactctccg gcgagccaat caccgcaccc aaccgcatga cccagacgct gccggcgcgc
    33241 tatggcttcg acgagaacac cctctacctc tcgccggcac cgctctatca tgccgcgccg
    33301 ctcggctaca accgctgcgt gctctgcgtc ggcggcaccg tgatcatcat ggagaagttc
    33361 gaccccgaag aggcgctgaa gctgatcgag aagtaccgca tcacccacag ccagtgggtg
    33421 ccgacgatgt tcgtgcggat gctgaagctg cccgaag
//
LOCUS       DAORCQ010000016        33395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_689_length_33395_cov_6.116827, whole genome shotgun sequence.
ACCESSION   DAORCQ010000016 DAORCQ010000000
VERSION     DAORCQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 33395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33395
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_689_length_33395_cov_6.116827"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1371
                     /locus_tag="PKI23_02775"
     CDS             262..1371
                     /locus_tag="PKI23_02775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN35896.1"
                     /translation="MNFQFDEDQDALRSAARKLLTSKATLEAFHKHMDQGSDYDRELW
                     ALMAENGYQGIGIPEAYGGAGMGYINQVVVADELGYVLAHTPYTSSIVGAAEAIMLAG
                     SEAQKQKYLPKLASGEWIGTFAYCEQNGRYDAEGVTAQASAGKISGTKMPVTDAACAN
                     FAVVAARGAAGVSLYLVDLKQSGVTIEPLRSMELTRKQAAVKFNGATAELLGGEGQGA
                     QLLDALYDRMAILVAFEQLGGAQRCVEMARDYSLERYIFGRPLAMYQGIKHRISDMHA
                     KIELARSNCLYGAWALDSSPADIAEAAALARSTLCDAYEFAAQENLQVHGGIGFTWEG
                     NCHMFVKRMRHLEALLGGRILWREKLLKALERKVA"
     gene            1423..2685
                     /locus_tag="PKI23_02780"
     CDS             1423..2685
                     /locus_tag="PKI23_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN35897.1"
                     /translation="MDFNDTPQEAAFRAEARAWLDQNAVLKDPKKSERYGMFSENESK
                     EMVKQAQAWQAKKYDAGWACITWPKQYGGRGGTVMENIIFSQEERRYETPPNVFGIGL
                     GMAGPTIMYHGTEEQKKRHLPTMARGDIIWCQLFSEPSAGSDLAALRTRSERQGDEWV
                     INGQKVWTSGAHYSKWGILVTRSDFNAPKHKGLTYFVVDMESPGIEIRPIKQMTGGAN
                     FNEVFFTNVRIPDANRLDREGNGWAVAITTLMNERMSIGTGMGLTPDAAYTALTALAK
                     NCYVNGKPAIENGLVRSKIGEFYSRLKALELTGYRVLSKLSSGAAPGTESAVLKLSGG
                     ILLQELSAFAMELQGPFGADHGAGAQFGGGLFQDIYLGIPAMRIAGGSDEVQRNVIAE
                     RELGLPQEARIDKNVPFKEIPTGSAGVR"
     gene            2825..3391
                     /locus_tag="PKI23_02785"
     CDS             2825..3391
                     /locus_tag="PKI23_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN35898.1"
                     /translation="MNRFVYDGKRRLYFLLGLALLLVAGYFLYFRDLNALQEALDAYQ
                     QGDFPRAMALYNELAAQQNPTALFSLGVMAERGEGGPVDRKQARIWYQRAADAGNQFA
                     QYNLGQMFSLGRSDERQPEQAVRYYQMAAEQGYDLAQLRLGQIYAHGDGVSRNLSEAR
                     RWFERAAKQDNPLARLELSKLERTDDAP"
     gene            3536..4093
                     /locus_tag="PKI23_02790"
     CDS             3536..4093
                     /locus_tag="PKI23_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNN35899.1"
                     /translation="MGWHNSPKRYGLTAQLFHWVTLLLVVALFALASYFEEMPAGPEK
                     GVVIGWHKSIGITVMLLTLLRLGWRLLDTAPPPVFAPTQWQQSLTLWTHRGLYLLLIV
                     LPFSGYLMSLAAGRPVVLFNQIALPVLLGENKPLAEQLKELHEILATLLFLLVALHSA
                     AALWHQFFLRDSLLSRMWPGGDDQA"
     gene            4153..4545
                     /locus_tag="PKI23_02795"
     CDS             4153..4545
                     /locus_tag="PKI23_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNN35900.1"
                     /translation="MLWIDLVTVLAILEFLTFGMLVGRARGLYGVAAPATSGHEMFER
                     YYRVQMNTLEQLVAFIPALWLAARYWSPEWVALVGAVFLVGRVIYLRAYVREPASRSL
                     GFALSFLPTVGLLLSVLVGVAMKGFSAG"
     gene            4554..5009
                     /locus_tag="PKI23_02800"
     CDS             4554..5009
                     /locus_tag="PKI23_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HNN35901.1"
                     /translation="MQIWVDADACPGAIREILFRAAQKRAVTVTLVANQAIRLPPSPH
                     LKMVCVPAGFDMADQRIADAMQPGDLVVTADIPLAAQVIDRGGVALNPRGQLYTPENM
                     RQQLSLRNFMSELRDSGVRTGGPATLSPRERQQFANQLDAWLTRRAGNG"
     gene            5126..5749
                     /locus_tag="PKI23_02805"
     CDS             5126..5749
                     /locus_tag="PKI23_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNN35902.1"
                     /translation="MHSAVEPNDYHHGNLKQALFEGALRQLRQQGAERLSLRALAREI
                     GVSQTAPYRHFKDKTELLQALAVEGYRNLRACNLAAIRLAGDDLRAALRAAACSYIRF
                     AKENSELFDLMFGGLVRCRANPQLDEAGAAALGVISELMEQGIRQGLLQNRESHAMAE
                     TAWGLAHGFATIFRNKQVDWDTAAQMRHVESAFDLLLDGLCVDPPRG"
     gene            complement(5756..6094)
                     /locus_tag="PKI23_02810"
     CDS             complement(5756..6094)
                     /locus_tag="PKI23_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020192100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNN35903.1"
                     /translation="MGPLNLLTDLPSQLAEERVEILVEHPGLRIERIVSTGQASPPGF
                     WYQQPEHEFVLLLQGTASLTLEREDGALQQHRLQPGDWLLLPAHCRHRVDSTAADTPT
                     VWLAVFQMAT"
     gene            6242..6607
                     /locus_tag="PKI23_02815"
     CDS             6242..6607
                     /locus_tag="PKI23_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013785949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HNN35904.1"
                     /translation="MATSVATAAVGSEQVVLTKALLNAGKALGLTQAQLGQVIGKDRT
                     AISRGGVDPDSKAGELALLLIRCYRSLYVLVGGDVAQMRHWMQSENRHTGGIPAQQIR
                     QVQGLLTVLEYLDAMRAKV"
     gene            6610..7359
                     /locus_tag="PKI23_02820"
     CDS             6610..7359
                     /locus_tag="PKI23_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNN35905.1"
                     /translation="MEIWTACQAEVTLRPLDGILLRMVESQEQVATTALVDDLTEQAL
                     LERMLERTKPAPRPGTGRLHYLLATPWRYPPLRHGSRYGRRSEPSLFYGSLSLTTLLA
                     EVAYYRLLFRAGMVQPATAIYRTQHTQFSASYRTEQGLSLQRPPFDRYRDWLISPDDY
                     SATQPLGSRLRAAQVMAFEYPSARDRAGGINIALFEPGALAVERPLEQEEWLCETSDQ
                     QVLFRSRSQPQPIIHHRDAFTVAGVLPAPAS"
     gene            7470..9050
                     /gene="gpmI"
                     /locus_tag="PKI23_02825"
     CDS             7470..9050
                     /gene="gpmI"
                     /locus_tag="PKI23_02825"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNN35906.1"
                     /translation="METTRKPTVLTILDGWGHREACEANAIALADTPVWDRLWREAPH
                     GLISGSGHDVGLPEGQMGNSEVGHMNLGAGRVLHQDLTRIDLAIEQGTFADNPVLAAA
                     IAQAVQGDRALHVLGLLSPGGVHSHEDQIAALLEAAAQRGVQRLYLHAFLDGRDTPPR
                     SAAPSLRRMENLLAGFQGADCRIASVIGRYYAMDRDQRWERVEQAYALIVDGQGRHQA
                     ASATAALEAAYARGEDDEFVTATRISDADHPPVTMQDGDLVIFMNFRADRARQISYAL
                     TRDDFSGFVRARRPKLGAFVTLTRYAEDLAAQVAYLPDRPVNVLGEYLSSLGKTQLRI
                     AETEKYAHVTFFFSGGREVPFAGEERILIPSPKVATYDLQPQMSAEAVTDQMVAAIGS
                     GRFDLIVCNYANGDMVGHTGKLEAAILAVQTLDHCLGRLIEAIDASGGEMLITADHGN
                     VEKMLDDETGQPHTAHTSDLVPLVYVGPRTLQIKAGGVLADVAPTLLELMQLPIPEEM
                     TGHSLIEPVQPQRHLARG"
     gene            9050..>9450
                     /locus_tag="PKI23_02830"
     CDS             9050..>9450
                     /locus_tag="PKI23_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35907.1"
                     /translation="MTTLALLQRCRPWLVILWLLLASASASAEEGTTRQRIEQVRQGI
                     GEATRWLKDAVGQGDAITRQLRNSELALAEHKAALAASATQIRQHEAAIAELDGRRQQ
                     LQQQLDEQKQQIAEQLRLAQRSGQDEPLKLLL"
     assembly_gap    9451..9550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9551..10518
                     /locus_tag="PKI23_02835"
     CDS             <9551..10518
                     /locus_tag="PKI23_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNN35908.1"
                     /translation="QLRLAQRSGQDEPLKLLLNQQDPSQVARLMSYYGYLSRAQNSRI
                     EAYRRNLAELDALEPRIRQQQEALQAAQRQQQAQLASLEQERTRHSAALNRLQSSIVD
                     KQSELKRLEKDRAELQQVLEAILAANREAERKRAEAARRQAEAEQKQAQQAKARAERA
                     QQAEKSASAKAEAPRHDEEAPAREVVASPRGGFARGQLPWPVRGKLLYRYGSAGAGGI
                     ARQGILIAAQEGSPVTAVQGGRVVFANWLRGTGLLLIIDHQNGFMSLYAHNQSLGKRV
                     GDAVRSGEPIGTVGSSGGQRSSGLHFEIRYQGQPQDPLTWLTPPG"
     gene            10572..11867
                     /locus_tag="PKI23_02840"
     CDS             10572..11867
                     /locus_tag="PKI23_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNN35909.1"
                     /translation="MAFKPHRPLLLLLLCAVVVRPMAAPSEPRDPLPLDDLRIFVEVF
                     DRIRTAYVHEVDDRTLLRNAIKGMLNELDPHSSYLDAESFKELEVGTTGEFGGLGIEL
                     GVEDGFIKVITPIDDTPAQRGGIEAGDIILKIDERSMKGVDLSEGVKLMRGEVGSKVT
                     LTIMREGEQQPRQFTLARDTIKLPSIKSRLLESGYGYLRIAQFQANTGTDLLKALEKL
                     RKEAKPLRGLVLDLRNNPGGLLDAAVAVADAFLSDGLIVYTQGRLSDSRMTYGATPPN
                     PAGEMPVVVLTNGGTASAAEIVAGALQDQHRAVIMGTSSFGKGSVQTILPLTEETGVK
                     LTTALYYTPSGRSIQAEGIQPDIVVERARITRLKEDDRSIKEQDLAGHLLNGSHKNEP
                     SSSRKKPAEQDELDGDYQLQEALNLLKGLAILNRPLKGG"
     gene            complement(11901..12284)
                     /locus_tag="PKI23_02845"
     CDS             complement(11901..12284)
                     /locus_tag="PKI23_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35910.1"
                     /translation="MKLGFFGRAARLLLQQRLLSATWLHCERQGLIAYLKLLQAVRLG
                     MAGALLLLCAAQMLVAGLIGGTVTALFLFVDEPRTRLWLLLGFFGLLLLLSLIAIGTL
                     LSQRLWLKHSGAARQIAQLKGQEPP"
     gene            complement(12281..12553)
                     /locus_tag="PKI23_02850"
     CDS             complement(12281..12553)
                     /locus_tag="PKI23_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35911.1"
                     /translation="MREPNSLSDAITQLQAQGGSTITDEAKALLRRDIERLRQTVAEL
                     KAELGIDAQTLQQQVEQSTAERPWTALGLAGLAGLMLGLLLRSGRK"
     gene            complement(12695..13156)
                     /locus_tag="PKI23_02855"
     CDS             complement(12695..13156)
                     /locus_tag="PKI23_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35912.1"
                     /translation="MHDLYAASKENQTFLHARFALGDDVLQPYKSTLDRWLWPDAFKN
                     QDTSVAKAKKAIADYRKAVGQPDGLAELMVFYCERAAGFSHDIGLQDEGYFDALVRMF
                     GQALTAIAVLPDAARPALLERLAVVRNISRDLGYGVSDDMDELLEAHGGDD"
     gene            complement(13327..13494)
                     /locus_tag="PKI23_02860"
     CDS             complement(13327..13494)
                     /locus_tag="PKI23_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35913.1"
                     /translation="MKNAFHDGAIPTTGTAITKVLPPGSRFSRENGHGLKKQAVLDKL
                     AAFFECYFGLA"
     gene            complement(13491..15053)
                     /locus_tag="PKI23_02865"
     CDS             complement(13491..15053)
                     /locus_tag="PKI23_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN35914.1"
                     /translation="MAAHWIEQLLNPACYPHPTGRIELIETHISWVILTGELAYKVKK
                     PVNFGFVDFSTLALRKRFCEEELRLNRRFTLSLYLRVVPITGTPQAPRIGGTGEVLDH
                     AVVMRQFDQSALLDRQLAAGQLSLEEAFRLGALIAEKHQLLAEAAADSAHGSAARVMA
                     PVRENFAQLRPRIDDPVDAARLLRLERWSEAEGKRLGRHFTARKAAGCVRECHGDLHL
                     GNIARIQGEFVLFDCLEFNADLRFIDVQSELAFVTMDLTDRGHPELAAGLLNGYLEAS
                     GDHEGLLLFNFYQVYRALVRAKVALLRAEQLQGAARQQAVQQGSHYLRLAEQFIQPRH
                     PWLGLAHGLSGSGKSHLALYIASQTGAVRLRSDVERKRLHGLDRTQRGGAAAGLYSAE
                     ATQRTFARLAELARTLIAAGFAVIVDATFLHRPQRATFAAIATELGVRRIILSISAEL
                     ATLVSRLKARASDPNEVSDANLAVLESQLKTQEPLTADELADTITLNAERLPAISELV
                     EEIERRAQTPAQ"
     gene            complement(15068..16354)
                     /gene="hemL"
                     /locus_tag="PKI23_02870"
     CDS             complement(15068..16354)
                     /gene="hemL"
                     /locus_tag="PKI23_02870"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNN35915.1"
                     /translation="MTHSETLFSAAQQVIPGGVNSPVRAFRSVGGTPRFIERGEGAWL
                     IDVDGRRYVDYVCSWGPLILGHAHPAVVAAVESRLRLGFSFGAPTALETELAEKIRAL
                     IPSIEQVRMVSSGTEATMSAIRLARGFTGRDKIVKFEGCYHGHGDSLLVKAGSGALTF
                     GVPSSLGVPKALAELTLTLEYNNLAQLEALFAAQGSEIACVIIEPVAGNMNCIPPLPG
                     FLEGLQHLCNRHGALLIFDEVMTGFRVALGGAQARYGITPDLTVLGKVIGGGLPVGAF
                     GGRRDVMARLAPLGPVYQAGTLSGNPLAMAAGLATLQQLEQPGVIARLEAQTSALSQG
                     LAELARSAGIPFITQHVGSMFGLFFTTQPEVSSYDQATRCDSARFNRFFHAMLDEGVY
                     LAPSAFEAGFLSTAHGAAELEHTFAAARRVFARLME"
     gene            complement(16351..16983)
                     /gene="thiE"
                     /locus_tag="PKI23_02875"
     CDS             complement(16351..16983)
                     /gene="thiE"
                     /locus_tag="PKI23_02875"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNN35916.1"
                     /translation="MIRGLYAITPTTTDDARLLQWVEAALKGGAQLVQYRDKSTDAQR
                     RRQQAEALLALCRKQGRPLIVNDDLQLALDIGADGLHLGREDGDLATARRQLGPTALI
                     GATCHQRLDWAETAVAAGASYVAFGRFFASRSKPQAPPAPLGLLHEARRRLDVPIVAI
                     GGISVDNARSVIEAGADAIAVIEGLFGSHDIAQRALQFQRLFIEPQAARP"
     gene            complement(16980..17756)
                     /locus_tag="PKI23_02880"
     CDS             complement(16980..17756)
                     /locus_tag="PKI23_02880"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNN35917.1"
                     /translation="MRPLILCLGGHDPTGGAGIQADAEAAAAAGCHALTVVTALTVQN
                     SHDIARIEPVPAGWITAQTRCLLDDFAISAIKIGLLGSTAAIDEVAALLETALSRLPL
                     VVDPILRSGGGHLVTDHAYLDALRQRLLPRATCLTPNREELFQLAGVDDEASAVGRLL
                     DRGCEHLLVSGGHDGDTPWLENRLYGRQGLIQRDRWPRLPHRFHGSGCTLAALLCAQI
                     ALGQPLPTAVRLSSEQSHRAMVLAEPLGGGQWIPRRIAPR"
     gene            complement(17770..18192)
                     /gene="hemJ"
                     /locus_tag="PKI23_02885"
     CDS             complement(17770..18192)
                     /gene="hemJ"
                     /locus_tag="PKI23_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HNN35918.1"
                     /translation="MLWLKAFHLIAVVCWFAGLFYLPRLFVYHSMSEDQPSRDRFKVM
                     ERKLYRGIMTPSMVATVALGLAMLLPNWESYRTAYWMHAKLTLVLILLAYHLYCGRLL
                     QRFAADQNRHGEIFYRWFNEFPVLILLPVVILVIVRPF"
     gene            18348..19388
                     /gene="argC"
                     /locus_tag="PKI23_02890"
     CDS             18348..19388
                     /gene="argC"
                     /locus_tag="PKI23_02890"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNN35919.1"
                     /translation="MVKVGIVGGTGYTGVELLRLLARHPEVSVELITSRGEAGIRVDQ
                     IYPNLRGFYDLAFVAPDPAALSELDVVFFATPHGVAMEMAPALVAAGVRVIDLSADFR
                     LKDPLLFQKWYGMPHSATDLLQEAVYGLPECNRAQIRQARLVAVPGCYPTAIQLGLLP
                     LLEAKLVDERSLIADAKSGVSGAGRQGKIANLLCEVADSFKAYGVPGHRHLPEITAHL
                     GQYLGCAAPGLTFVPHLAPMIRGIHATLYARLKDPAVQPDLQQLYAERYRDEPFVDLL
                     PAGEHPETRSVRGANLCRLALHRPQGGDTLVVLSVIDNLVKGAAGQAIQNMNLCCGLP
                     ETSGLDAPALLP"
     gene            19479..19832
                     /gene="erpA"
                     /locus_tag="PKI23_02895"
     CDS             19479..19832
                     /gene="erpA"
                     /locus_tag="PKI23_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNN35920.1"
                     /translation="MSSVQVQQPVALEVTAAAARKVRRLVDEEGNSQLKLRVFVTGGG
                     CSGFQYGFSFDDEAAEDDTRIERDGITVLVDPMSFQYLVGSEIDYSEGLEGSRFVVRN
                     PNATTTCGCGSSFAV"
     gene            complement(19842..20978)
                     /locus_tag="PKI23_02900"
     CDS             complement(19842..20978)
                     /locus_tag="PKI23_02900"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNN35921.1"
                     /translation="MSTEERSDGDCYLGVMSGTSLDAVDVGLFRFTDTSCLLLAKHSL
                     PIPADIRQEAIALATPGPDELNRLHRLDIDHAELIATAVNQLLAQCGIDRARIRAIGS
                     HGQTLRHSPGGDHAFTCQVGDPNRIAYLTGLVTVADFRRKDMVAGGEGAPLAPAFHHW
                     LFSHPTEYRMVLNLGGIANLTVLPPTQHPAAVVGFDTGPGNALLDGWCQRHLGQPYDH
                     RGNWSRSGSCHEPLLRALLDDAFFQATPPKSTGRERFNLNWLTQRLRDFAHCAPVDVQ
                     ATLLELTARSVAQAIDRWGCGTELYLCGGGVENRALVDRLQAALPGRRIHSTELLGLP
                     PQWVEAATFAWLARQRLLGRPGNLPSVTGARRPVMLGGVYLPDI"
     gene            complement(20975..22288)
                     /locus_tag="PKI23_02905"
     CDS             complement(20975..22288)
                     /locus_tag="PKI23_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNN35922.1"
                     /translation="MKTAFPRLHLIAATLLLSATVLIAWLLPSEEAAAIKSVVPLALP
                     APTTPQSTEQPAVAVTTALVEPELWDEYVVQTGDTLGTLFKRAGIAPNELAMLLDSGK
                     ETKRLHQIHPGHKLALQVGTEGQLERFKFSPNALESVQWQRTNDSFDVTVEKREPELQ
                     LRRHSGVIDQSLFLSCQKAGLSQAVASQLADLFGWDIDYTEDLRQGDRFSVIYQERFI
                     DGKKIDDGPILAAEFINQGRRYQTVRHVDAQGNEGYYTPDGRGMRKAFIRNPLDVVRI
                     TSLFSLSRFHPILHTLRAHKGIDYSASAGTPVKSVGDGRVTFAGRKGGYGNTITIQHG
                     DRYTTLYAHLQGFAPSIRNGQKVRQGEVIGYVGASGLATGNHLHYEFQVDGVHRNPLT
                     VPLPQAPAVAGRELDRFKSQTGPLVAMLKQGESTPAVVARAGLND"
     gene            22533..23744
                     /gene="tyrS"
                     /locus_tag="PKI23_02910"
     CDS             22533..23744
                     /gene="tyrS"
                     /locus_tag="PKI23_02910"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNN35923.1"
                     /translation="MTSVKAALELIKAGAHEIISEPELIEKLGSGRKLRVKAGFDPTA
                     PDLHLGHAVLLNKLHQFQQLGHEVLFLIGDFTGMIGDPTGKNVTRKPLTRQQVEQNAQ
                     TYSEQVFTVLDRAKTQVVFNSQWMGRFDASDMIKLASRYTVARMLERDDFHKRYRGGQ
                     PIALHEFLYPLIQGHDSVELRADIELGGTDQKFNLLVGRELQRDAGQTPQVVMTMPIL
                     EGLDGVQKMSKSLDNYIGLREPPNTMFGKLMSISDTLMWRYYALLSRRLPTEVAAMQA
                     QCEAGELNPRDAKVELARELVTRFHSAAEAEAAHQAFVARFQQGALPQEIPELCIAHG
                     GYPAEGVPIANLLKEAGLVASTSEALRLIGQGGVRIDGIRLQEKNHRVAPGSEHLYQA
                     GKHRFARIRVN"
     gene            23792..>24048
                     /locus_tag="PKI23_02915"
     CDS             23792..>24048
                     /locus_tag="PKI23_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HNN35924.1"
                     /translation="MYKLVFFVPIEQLEAVKAALFAAGGGRIGHYDCCAWQVLGLGQF
                     RPRAGSNPFLGSQEQLEQVPEYRVEMVVADALIEPVIRALHA"
     assembly_gap    24049..24148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24149..25817
                     /locus_tag="PKI23_02920"
     CDS             <24149..25817
                     /locus_tag="PKI23_02920"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNN35925.1"
                     /translation="LQLKGELSMEMHFATLWEQIAAQIPQRTALIHGERKVAWGAYQE
                     RAARIAQFLHQQGLGHDSKMGLYLYNSPEYMEAQFAGFKVRGVPVNVNYRYLEDELVY
                     LLDNSDSEALFFHGRFAERIANIRHKLPKVRCLIQVEDGSGAPLLAGAHDYEQIIRTH
                     APMAPIERGWDDTYMLYTGGTTGMPKGVMYKLGEMCAGFLAPFEARGVSPPPATIEET
                     LAAIKRLEPAGLLPVTLVACPLMHGTGMWLGAMMPHNMGGTVVTLAGTKFDADELWAA
                     VQRERVTDIVIVGDAFAKPMLDALRAAQAAGRPYDISSVRFISSSGVMWTQEVKAGLL
                     EFGDMILNDGLGSTEGGMGRSLTTRANIGETAKFQMMESTKVFDDNDEEVKPGSGAIG
                     MIAQAGRVPTGYYKDPEKSAKTFRTIRGLRYSFPGDFATVEADGTIRLLGRGSMCINT
                     MGEKVYPEEVEEAIKTHPAVYDCLVVGVKDPKYGERVAAVVSLRPGQQADQQTLIDHV
                     AHRLAGYKKPRNIFIVDEVQRASNGKADYKWARSRADEEARKLEMGG"
     gene            25818..26738
                     /gene="prmB"
                     /locus_tag="PKI23_02925"
     CDS             25818..26738
                     /gene="prmB"
                     /locus_tag="PKI23_02925"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNN35926.1"
                     /translation="MVAMAPTAAFQPDFEAAQSELLTLRDFLRWGVSSLGAAQVALGH
                     GCDDLWDEALRLILHALHLPLEVDDRVLDARLLPRERQQVVELFRRRIVDRLPNAYLT
                     GEAWFAGLPFFVDQRVLIPRSPLAELIARRFEPWIDSSRVDRVLDLCTGSGCIAIALA
                     HAFEAAEVVATDLSAAALEVAAINRARHGLEQRLTLIEGDLFSGVTGTFDLIVSNPPY
                     VAEQELAALPAEYRHEPRLALAAGVEGLDIVERILAQARRHLRPGGLLVVEVGSAAEA
                     LVARHPDLGFFWPDFEQGGEGIFILEADQL"
     gene            complement(26772..27335)
                     /locus_tag="PKI23_02930"
     CDS             complement(26772..27335)
                     /locus_tag="PKI23_02930"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNN35927.1"
                     /translation="MKFCTHCGEAIVLKVPESDNRERHVCGDCGTIHYQNPKIIAGCL
                     ITQNDQVLLCRRAIEPRLGFWTLPAGFMENDETTQEAAARETWEEANARVRVGDLYTL
                     FNLVHINQIYLFFRGELIEPGFAAGKESLEVKLFAREEIPWEMLAFAVIRKTLEFHFA
                     DRERGDYPLRMQDIRAADPRFFQPKPL"
     gene            complement(27422..27769)
                     /locus_tag="PKI23_02935"
     CDS             complement(27422..27769)
                     /locus_tag="PKI23_02935"
                     /inference="COORDINATES: protein motif:HMM:NF022777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HNN35928.1"
                     /translation="MPTFTPLPAPEQLRQLAEQDPQALEALRLQLVDELIAQAPESRQ
                     QRLRGLQFQIDMERRRAKTPMAACIKLSQMMHDSLYTLNLALKGEPLPTSQQARAEPT
                     ATVTPFRTREARA"
     gene            27768..28244
                     /locus_tag="PKI23_02940"
     CDS             27768..28244
                     /locus_tag="PKI23_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35929.1"
                     /translation="MAVAPGEGRQTVHERKICPRDLLKLKSSLPGAVELINICTDCGW
                     RWCGTGIFWCFCRNIAAKSVWMLAVERSCLHGSQSIHLHNRGIGKNIFLFESRNRRFF
                     VLSRPVLAEIPTLLAALACRRLWVDWFRMAADSKQPCPASSLINPPWRENSDLPSG"
     gene            28225..28761
                     /locus_tag="PKI23_02945"
     CDS             28225..28761
                     /locus_tag="PKI23_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNN35930.1"
                     /translation="MIYRLGERQLEVCGDEFFVADSAVVIGSVRLERNASIWFNAVLR
                     GDNDLITIGEDSNIQDGSVLHTDHGVPLTVGRGVTVGHKVMLHGCTIGDYSLIGMNAV
                     VLNGARIGKYCLIGANTLIPEGKEIPDGSLVMGSPGKVVREISDLQRKILEGSAQHYV
                     ENSRRYTQQLRLDPRFHG"
     gene            28772..28981
                     /locus_tag="PKI23_02950"
     CDS             28772..28981
                     /locus_tag="PKI23_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNN35931.1"
                     /translation="MGERPPPSPCVSVCALDEMDICIGCHRSVDEIAAWSQLSAAQKW
                     QVIQLSQARVRAAGGWLIPPDGAEP"
     gene            28978..30159
                     /gene="purT"
                     /locus_tag="PKI23_02955"
     CDS             28978..30159
                     /gene="purT"
                     /locus_tag="PKI23_02955"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNN35932.1"
                     /translation="MTEIGTPNSPTALRVLLLGSGELGKEVAIELQRLGVEVIAVDRY
                     ADAPAMQVAHRAHVISMLDGAALRRVVELEQPHYIVPEIEAIATDELLKLEAEGHRVV
                     PTARAARLTMDREGIRRLAAEELGLPTSPYGFADSEAELIEQVARIGFPCVVKPVMSS
                     SGKGQSVAHDQAGLIKAWRYAQEGGRAGAGRVIIEGFVRFDYEITLLTIRHRDGTTFC
                     APIGHRQEAGDYRESWQPQRMSRAALERSEAVAAKVTEALGGFGLFGVELFICGDEVY
                     FSEVSPRPHDTGMVTMISQDLSEFALHARALLGLPIPNVRQLGPAASAVILVQGASQQ
                     PSYGALEVALAEPDTQLRLFGKPDVAGQRRMGVGLARADSVEAALEKAKRVAAAVRVR
                     L"
     gene            30202..30867
                     /locus_tag="PKI23_02960"
     CDS             30202..30867
                     /locus_tag="PKI23_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNN35933.1"
                     /translation="MKPTLYGAPLSPFVRKARIVLAEKGIDYDLVPLDPFRKSEELLR
                     LSPLGRVPALVDDGKTLADSGVIAQYLEKKWPTPPLYADDPYLHARVVWFEKYADYEL
                     VPLCTFSVFRQRLLMPMMGQGCDEQLVQSALQKLPAHFDYLEQQLDGADHLIDNRLTV
                     ADIAVASQCINMGHGGECVDAQRWPTLAALVERVKARPSAADCLAQEAAIIDKIRART
                     AGG"
     gene            complement(31221..31838)
                     /gene="pdxH"
                     /locus_tag="PKI23_02965"
     CDS             complement(31221..31838)
                     /gene="pdxH"
                     /locus_tag="PKI23_02965"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNN35934.1"
                     /translation="MTTLFPPLNEHEAPPHPWGLFREWYDAARPLHPPEPDAMTLATV
                     GVDGRPSARIVLLKQFDEDGLVFHTHYESRKGAELSHNPSAALLFYWPVQLRQVRIEG
                     GVERIDPAESDAYFRTRPQGSQIGAWASPQSRVLQGREELDARIDRFTRDFADREIPR
                     PPGWGGYRLLPLRFEFWQARIDRLHDRLLYQRRPDQTWSLKRLAP"
     gene            32272..32754
                     /locus_tag="PKI23_02970"
     CDS             32272..32754
                     /locus_tag="PKI23_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNN35935.1"
                     /translation="MSSDLLPLRVDPFKLVKSGAVLEGRLQMGRFRRFCAELTGDEGQ
                     LFVQLQAGRDEQGVACLAGRLTGEVGLICQRCLEPMRHPIDVPFRLALLQSEASIERL
                     PDSYDPLVLPAEAGAVEWPVLLEDELLLSLPIIPRHPWAECSAPGEYFAAADRTDPAQ
                     "
     gene            32801..32983
                     /gene="rpmF"
                     /locus_tag="PKI23_02975"
     CDS             32801..32983
                     /gene="rpmF"
                     /locus_tag="PKI23_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNN35936.1"
                     /translation="MAVQQNRVTRSKRDKRRSHDALTPPTLSVDSVTGETHRRHHVSA
                     NGYYRGRKIVSGKDDD"
     gene            32993..>33395
                     /locus_tag="PKI23_02980"
     CDS             32993..>33395
                     /locus_tag="PKI23_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNN35937.1"
                     /translation="MRTVAIDVMGGDHGPAVTVPAALQSLSRDPLLHLLLVGRQEQIT
                     PFLRDVPAALHERLQTVDARDVVAMDERPSVALRSRKPSSMWLAIECVQSGRAQACVS
                     AGNTGALMVIGRHLLKMVAGIDRPAIITQIPT"
BASE COUNT         5854 a        10223 c        11147 g         5971 t
ORIGIN      
        1 tacgacctcg gtctcgacgg cgtcttcgcg gcggagcggg cgcgcggggc cgcggccccg
       61 ggcgtgccgg agccatgccg gccgcggccg agcggtctgt ttattccatc accgcagttg
      121 cggtacccta ggtcgcgatc aagcaattgg tcgaagggtg catgggcgat ctggccggtt
      181 tgtctttgaa tcatcgggtc agtgtggatc gccatgcgca ggtgagcgga accccaacaa
      241 gaaagaggga gtaaagagtc aatgaacttt caattcgacg aagaccagga tgccctgcgc
      301 agtgcagcac gcaagctgct gaccagcaag gccaccctgg aggcattcca caagcacatg
      361 gaccagggca gcgactatga cagggagctg tgggcgttga tggccgagaa cggctatcag
      421 ggcatcggca ttcccgaagc ctatggcggt gccggcatgg gttacatcaa ccaggtggtg
      481 gtggccgacg aactgggcta tgtgctcgcc cacaccccct acacctcttc gatcgtcggt
      541 gcggccgagg cgatcatgct ggccggcagc gaagcgcaga agcagaagta cctccccaaa
      601 ctggcgtcgg gtgagtggat cggcaccttc gcctactgcg agcagaatgg ccgctacgac
      661 gccgagggcg tgaccgccca ggccagtgcc ggcaagatct ccggcaccaa gatgccggtc
      721 accgacgcgg cctgtgccaa tttcgccgtg gtggcggcgc gtggcgcggc cggtgtctcg
      781 ctctatctgg tcgacctcaa gcagtcgggt gtcaccatcg aaccgctgcg cagcatggag
      841 ctgacccgca agcaggcggc cgtcaagttc aacggtgcca ctgccgagtt gcttggtggc
      901 gagggtcagg gtgcgcaact gctcgatgcg ctgtacgacc ggatggcgat tctggtggcc
      961 ttcgagcaac tgggcggcgc ccagcgctgc gtcgaaatgg cgcgcgacta ctcgctggag
     1021 cgctacatct tcggccggcc gctggcgatg taccagggca tcaagcaccg catctccgac
     1081 atgcacgcca agatcgagct ggcccgctcc aactgcctct acggcgcctg ggcactcgac
     1141 agcagcccgg ccgacatcgc cgaggccgcc gccctggccc gcagcaccct ctgcgatgcc
     1201 tacgagtttg ccgcgcagga gaacctgcag gtgcatggcg gcattggctt cacctgggag
     1261 ggcaactgcc acatgttcgt caagcggatg cgccacctcg aggccctgtt gggcggccgc
     1321 atcctgtggc gggaaaaact gctcaaggcc ctggagcgca aggtggcctg agtggccccg
     1381 gcgaccggtt gctgacaccc cgataatcaa aaggaacgga acatggactt caacgatacc
     1441 ccccaagaag cggcgtttcg cgccgaggcg cgtgcctggc tcgaccagaa tgccgtactg
     1501 aaagacccga aaaaatccga gcgctacggc atgttctccg aaaacgagag caaggagatg
     1561 gtcaagcagg cgcaggcctg gcaggccaag aagtacgatg ccggctgggc ctgcatcact
     1621 tggcccaagc agtatggtgg ccgtggcggc accgtcatgg agaacatcat cttcagccag
     1681 gaggagcgcc gttacgagac cccgcccaac gtcttcggca tcggcctcgg catggccggc
     1741 cccaccatca tgtaccacgg caccgaagag cagaagaagc gtcacctgcc gaccatggcg
     1801 cgtggcgaca tcatctggtg tcagctgttc agcgaaccaa gcgccggctc tgacctcgcg
     1861 gccctgcgca cccgctccga gcggcagggt gacgagtggg tcatcaacgg ccagaaggtg
     1921 tggacctccg gtgcccacta ctccaagtgg ggcattctgg tcacccgcag cgacttcaac
     1981 gcaccgaagc acaagggact gacctacttc gtggtcgaca tggagagccc gggcatcgag
     2041 atccggccga tcaagcagat gaccggcggc gccaatttca acgaagtctt cttcaccaac
     2101 gtgcgcatcc ccgacgccaa ccggctcgac cgcgaaggca acggctgggc ggtggccatc
     2161 accacgctga tgaacgagcg gatgtcgatc ggcaccggca tggggctcac ccccgatgcc
     2221 gcctacacgg cgctcaccgc gctggccaaa aactgctacg tcaatggcaa gccggcgatc
     2281 gagaatggac tggtgcgcag caagatcggc gagttctaca gccggttgaa ggcgctggag
     2341 ctgaccggct accgcgtgct gtcgaagctg tcgagcggtg ccgcacccgg caccgagagc
     2401 gcggtgctga agctctccgg cggcattctg ctgcaggagc tttcggcctt cgcgatggag
     2461 ctgcaaggtc cgttcggagc cgatcacggg gcgggtgccc agttcggcgg cggactgttt
     2521 caggacatct acctcggcat tccggccatg cgcatcgccg gtggttcgga tgaagtgcag
     2581 cgcaacgtca tcgccgagcg tgagctgggt ctgccgcagg aggcgcgcat cgacaagaat
     2641 gtgcccttca aggagattcc aaccggctcg gccggcgtgc gctgagcgcc aggcaaaccg
     2701 ccgatcgaag ggcccggcca cgccgggcct ttctttatgt cggggtcaga ggtttccagg
     2761 gcggccgcaa accggcatca tcacttcaac acactgattt tttgatcaaa tccgggttcg
     2821 atcgatgaac agatttgtct acgatggcaa gcgccggctt tactttctgc tgggtctggc
     2881 cctgctgctg gtggcgggtt actttctcta ctttcgtgac ctcaatgccc tgcaggaggc
     2941 gctcgacgcc taccagcagg gcgatttccc ccgggccatg gcgctctaca atgaacttgc
     3001 ggcccagcag aaccccaccg cgctcttctc gctcggcgtc atggccgagc gtggcgaggg
     3061 tggcccggtc gatcgcaagc aggcccgtat ctggtatcag cgcgccgccg atgccggcaa
     3121 ccagtttgcc cagtacaacc tgggacagat gttcagcctc ggtcgcagtg acgaacgcca
     3181 gcccgagcag gccgtgcgtt attatcagat ggctgccgag cagggctacg acctggccca
     3241 gcttcggctg gggcagatct atgctcatgg cgacggcgtc agccgcaacc tgagcgaggc
     3301 gcgccgctgg ttcgaacggg ccgcgaagca ggacaacccg ctggcgcggc tcgaattgag
     3361 caagctggag cgaacagacg acgcgccttg agcaactgag tttcaccaca gcgatctgca
     3421 gccatgacaa aaagactgac gatggcctcc cggagtgggc cgaatgcccg gagcagggta
     3481 gaatcgacct gactgcctcg cagaccacca aagtaataaa aaaggaggag tgacaatggg
     3541 ttggcataac agtcccaaac gctatggcct gacggcgcaa ctgttccact gggtcacgct
     3601 gctgttggtg gtggcactct ttgcgctggc cagttacttc gaggagatgc cggccggacc
     3661 cgagaagggg gtggtgatcg gctggcacaa gtcgatcggc atcacagtga tgctgctgac
     3721 gctgctgcgg ctgggatggc ggctgctcga cacagcgccg ccgccagtgt tcgccccgac
     3781 gcagtggcag cagagtctga cgctctggac ccatcggggg ctctatctgc tgctgatcgt
     3841 gctgccgttc agcggctacc tgatgtcgct ggccgcagga aggccggtgg tgctgttcaa
     3901 ccagatcgcc ttgccggtgc tgctgggcga gaacaagccg ctggccgagc agttgaagga
     3961 gctgcacgaa attctggcca cgctgctctt tctgctggtg gcgctgcaca gtgccgcggc
     4021 actgtggcat cagttctttc tgcgcgactc gctgctgtca cggatgtggc cgggtggcga
     4081 cgatcaggcg tgagcagggt cgcttgtaca gtgctgtttt tgctttccct ttcatcgact
     4141 ggagattgaa cgatgctgtg gatcgatctg gtgacggtgc tggccattct ggagttcctc
     4201 accttcggca tgttggtggg gcgggcgcgc ggtctgtatg gcgtggcggc gccggccacc
     4261 agcggccacg agatgttcga gcgctactac cgggtacaga tgaacaccct ggagcagctg
     4321 gtggccttca ttcccgcgct ctggctggct gcgcgttatt ggtcgccgga gtgggttgcg
     4381 ctggttggcg cggtctttct ggtgggacgg gtgatctatc tgcgcgccta tgtgcgtgag
     4441 ccggcgtcgc gcagtctggg gtttgccctc agttttctgc cgaccgtcgg gctgctgctg
     4501 agcgtgctgg tgggggtggc gatgaagggc ttcagcgccg gttgagcctg tcgatgcaga
     4561 tctgggtcga tgccgatgcc tgtccgggcg cgatcaggga gattctgttt cgcgctgcgc
     4621 agaagcgcgc ggtcaccgtg acgctggtgg ccaaccaggc cattcgtctg cccccttcac
     4681 cccacctcaa gatggtctgc gtgccggccg gcttcgacat ggccgaccag cggattgccg
     4741 atgcgatgca gccgggtgat ctggtggtga ccgccgacat tccgctggcg gcacaggtga
     4801 tcgaccgcgg cggcgttgca ctcaatccgc gtggccagct ctacacccct gaaaacatgc
     4861 ggcagcagct ttcgctgcgc aacttcatga gcgagctgcg tgacagcggg gtgcggactg
     4921 gcggtccggc cacgctgagc ccgcgggagc gacagcagtt cgccaatcag ctcgatgcct
     4981 ggctgacgcg gcgagccggt aacggttgat ggcctgacgg cgcttgggaa agcacgccag
     5041 aacggctaca atcctcgcca atgtgtgcga tgagtacatt gccatcgcac gctgttgacc
     5101 agcgataaca atcaagaccg tcatgatgca ttccgcagtc gaaccgaacg attaccacca
     5161 cggcaacctc aagcaggcgc tgttcgaagg cgcgctgcgc caactgcgcc agcagggggc
     5221 cgagcggctc agcctgcgcg cgctggcgcg ggagatcggc gtctcccaga ccgcacccta
     5281 tcgccacttc aaggacaaga ccgagctgtt gcaggcgttg gcggtcgagg gttaccggaa
     5341 tctgcgggcc tgcaatctgg cggcgatacg gctggcgggc gatgacctgc gggctgcgct
     5401 gcgggctgcc gcatgctcct atatccggtt tgccaaggag aacagcgaac tgttcgacct
     5461 gatgttcggt ggtctggtcc gctgtcgggc caatccgcaa ctcgatgaag ccggcgccgc
     5521 cgcgctcggg gtgatcagtg aactgatgga gcagggcatt cgccagggcc tgctgcaaaa
     5581 ccgcgagagc catgcgatgg ccgagaccgc ctggggactg gcgcatggct ttgcgaccat
     5641 ctttcgcaac aaacaggtcg attgggatac cgcagcgcag atgcgccacg tcgagagcgc
     5701 cttcgatctg ctgctggatg gcctctgcgt cgatccaccg cgcggctgat ccgcttcacg
     5761 ttgccatctg gaaaacggcg agccacaccg tcggggtatc ggcggcggtc gaatcgaccc
     5821 gatgccgaca gtgcgccggc agcagcagcc agtcaccggg ttgcagccga tgttgctgta
     5881 acgcgccatc ctcccgttcc agcgtcaggc tggccgtgcc ttgcagcagc agcacgaatt
     5941 catgctccgg ttgttgatac cagaaacccg gcgggctggc ctgcccggtc gagacgatcc
     6001 gctcgatgcg cagccccgga tgctcgacca ggatctcgac ccgttcttcc gccagttggc
     6061 tgggtagatc ggtcagcagg ttgagcgggc cgatgggctt caacggatga ttcatagcgc
     6121 gctcctttga tttatgtttc tgccgtgcat ctgcgtggaa gggtgttggc agccatcttg
     6181 atgtgctata tgctgcatta atcaatgcaa atgtgcatat attgcattct gggagcggcg
     6241 catggcaact tcggtggcaa cggcagcagt ggggtcggag caggtggtgc tgaccaaggc
     6301 gctgctgaat gccggcaagg cgctggggtt gacccaggcg caactgggac aggtgattgg
     6361 caaggaccgc acggcgatca gccgcggcgg tgtcgatccc gacagcaagg cgggtgaact
     6421 ggcgctgctg ctgatccgct gctatcgatc gctctatgtg ctggtgggtg gcgatgtcgc
     6481 acagatgcgg cactggatgc agagcgaaaa ccgccacacc ggcggcatcc cggcgcagca
     6541 gatcaggcag gtgcaggggt tgctgaccgt gctcgaatac ctcgatgcga tgcgggccaa
     6601 agtgtagccg tggagatctg gacggcctgt caggccgagg tgacgttgcg gccgctggac
     6661 ggcatcctgc tgcggatggt cgagagtcag gagcaggtgg cgaccaccgc cctggtcgat
     6721 gacctgaccg agcaggccct gctcgaacgg atgctcgagc gtaccaagcc ggcgccgcgc
     6781 cccggcaccg ggcggctgca ctacctgctg gccaccccct ggcgctatcc gccactgaga
     6841 catggctcgc ggtatggccg gcgcagtgag ccgagcctgt tctatggctc actgtcgctg
     6901 accacgctgc tggccgaggt ggcctactac cggctgctgt tccgggccgg catggtgcag
     6961 ccggccacgg cgatctatcg gacgcagcac acccagttct ccgccagcta ccgcaccgaa
     7021 caggggctgt cgctgcaacg gccgcccttc gaccgctacc gcgactggct gatcagcccc
     7081 gatgattaca gcgcgaccca gccactgggc agccggctgc gcgccgccca ggtcatggcc
     7141 tttgaatacc cctcggcgcg cgaccgcgcg ggcgggatca acattgcgct gttcgagccg
     7201 ggcgcgctgg ccgtcgaacg gccgctggag caggaggagt ggctgtgcga gaccagcgat
     7261 cagcaggtgc tgttccgcag ccgcagccag ccgcagccga tcatccacca tcgcgatgcc
     7321 ttcaccgtgg ctggcgtact gccggccccg gccagttgag tctcggtgtg gcaccgatgg
     7381 cgcgttgcgc ctgcgggtga cagcgcctag cattggcaat tgatctgttg aacagcgccc
     7441 tttcaccgat tttaccgaag gaatttcgca tggagaccac ccgcaagccc accgtgctga
     7501 ccatcctcga tggctggggc caccgtgagg catgtgaggc caatgccatc gcgctggccg
     7561 atacaccggt gtgggatcgg ctctggcgcg aggcgccaca tgggctgatc tccggttccg
     7621 gccacgatgt cgggctgccg gaagggcaga tgggcaactc cgaggtgggc cacatgaatc
     7681 tcggtgccgg tcgggtgctc catcaggatc tgacccgcat cgatctggcg atcgagcagg
     7741 gaacctttgc cgacaatccg gtgcttgctg cggccatcgc acaggccgtg cagggcgacc
     7801 gcgcgctgca tgtgctgggc ctgctgtcgc cgggtggcgt gcacagccat gaggatcaga
     7861 ttgccgcgct gcttgaggcg gcggcgcagc gcggggtgca gcggctctac ctgcatgcct
     7921 ttctcgatgg acgcgacacg ccgccgcgca gtgcggcgcc gtcgttgcgg cgcatggaga
     7981 acctgctggc cggttttcag ggtgccgact gtcgcatcgc ctcggtgatc ggtcgttact
     8041 atgcgatgga ccgcgatcag cgctgggagc gggtcgaaca ggcttatgcg ctgatcgtcg
     8101 atggtcaggg gcggcatcag gcggcgagcg cgactgctgc gctggaggcg gcctacgccc
     8161 gcggtgagga tgatgaattc gtcaccgcca cccggatcag cgatgccgac catccgccgg
     8221 tgaccatgca ggatggcgat ctggtcatct tcatgaattt tcgtgccgac cgcgcccgac
     8281 agatcagcta cgccctgacc cgcgatgact tctccggttt cgttcgcgcg cgccggccga
     8341 agctgggcgc cttcgtcacc ctgacccgct atgccgagga tctggcggcc caggtggcct
     8401 atctgccgga ccgtccggtc aacgtgctgg gcgaatatct gtccagcctt ggcaagacgc
     8461 agttgcgtat cgccgagacc gagaagtatg cccatgtcac cttcttcttc agcggcggac
     8521 gcgaagtccc cttcgccggc gaggagcgca tcctgattcc gtcaccgaag gtggccacct
     8581 atgacctgca accgcagatg agtgccgagg cggtgaccga ccagatggtg gcggccatcg
     8641 gcagcggccg gttcgacctg atcgtctgca actatgccaa tggcgacatg gtcggccaca
     8701 ccggcaagct ggaggcggcc atccttgcgg tgcagacgct cgaccactgc ctgggccggc
     8761 tgatcgaggc gatcgatgcc agcggcggcg agatgctgat caccgccgac catggcaatg
     8821 tcgagaagat gctcgatgac gagaccggtc agccgcacac cgcccacacc agtgacctgg
     8881 tgccactggt ctatgtcggc ccccggacgc tgcaaatcaa ggcgggcggc gtgctggccg
     8941 atgtcgcacc caccctgctg gagctgatgc aactgccgat tccggaggag atgaccggcc
     9001 actcgctgat cgagccggtg cagccgcaac ggcatctcgc ccgcggttga tgacgactct
     9061 ggcgcttctc cagcgctgcc gaccgtggct ggtcatcctc tggctgctgc tggcctcggc
     9121 cagcgccagc gccgaggagg gcaccacccg ccagcgcatt gagcaggtca ggcagggcat
     9181 cggcgaggcc acccgttggc tgaaggatgc cgtcggccag ggcgatgcca tcacccggca
     9241 actgcgcaac agtgagctgg cgctggccga acacaaagcg gcgctcgctg ccagcgcgac
     9301 gcagatccgc cagcacgaag ccgccatcgc cgaactcgac ggacggcgcc agcagttgca
     9361 gcagcagctc gatgagcaga agcagcagat cgccgagcag ttgcggctgg cccagcgcag
     9421 cggccaggat gagccactca agctgctgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn agcagttgcg gctggcccag cgcagcggcc aggatgagcc actcaagctg
     9601 ctgctcaacc agcaggatcc cagccaggtg gcgcggttga tgagttatta cggttacctc
     9661 agccgggcgc agaacagccg catcgaagcc tatcggcgca acctggccga gctggatgcg
     9721 ctcgaaccgc gcatccgcca gcagcaggag gcgctgcagg cggcgcagcg acagcagcag
     9781 gcacagctcg ccagcctcga gcaggagcga acccgccaca gtgccgcact gaaccggctg
     9841 cagagcagca tcgtcgacaa gcagagcgag ctgaagcggc tcgaaaaaga ccgtgccgag
     9901 ttgcagcagg tgctcgaagc cattctggcc gccaaccgtg aagccgaacg caagcgggcc
     9961 gaggccgcgc gacgtcaggc cgaggcggaa caaaaacagg cccagcaggc aaaagcgcgt
    10021 gccgagcgtg cacaacaggc agaaaaatcg gcttcggcca aggccgaggc ccccaggcat
    10081 gacgaagagg cacccgccag ggaggtcgtt gcgtcgccca ggggcggttt cgcccgtggc
    10141 cagttgccct ggccggtccg cggtaaactg ctctaccgct atggcagtgc cggtgccggc
    10201 ggcatcgccc gccagggcat tctgatcgcc gcgcaagagg gcagcccggt gaccgccgtg
    10261 cagggtggtc gggtggtctt cgccaactgg ttgcgtggaa ccgggctatt gctcatcatc
    10321 gaccaccaga atggcttcat gagcctctat gcccacaatc agagcctcgg caagagggtg
    10381 ggcgacgcgg tcaggagcgg cgagccgatc ggcacggtcg gatcgagcgg tggccaaaga
    10441 agcagcggtc tgcacttcga gattcgctat cagggccagc cccaggatcc gctcacctgg
    10501 ctgacgccac ctggctgagc cggtccgatc cattctcctg tctcctcacc tcctcactgg
    10561 aatgaccctc aatggccttc aaaccgcacc gtccgctgtt gcttctgctg ctctgcgccg
    10621 tcgtcgtcag accgatggcg gccccttcgg aaccgcgcga tccgctgccg ctcgatgatc
    10681 tgcgcatctt cgtcgaggtg ttcgaccgca tccgcaccgc ctatgtgcat gaggtggacg
    10741 accggaccct gctgcgcaac gccatcaagg ggatgctgaa cgagctcgat ccgcactcca
    10801 gctatctcga cgccgagagt ttcaaggagc tggaggtggg caccaccggc gagttcggtg
    10861 gtctgggcat cgaactgggt gtcgaggatg gtttcatcaa ggtgatcacg ccgatcgatg
    10921 acaccccggc acagcgtggc ggcatcgagg cgggtgacat catcctcaag atcgacgagc
    10981 ggtcgatgaa gggggtcgac ctgagtgaag gggtcaagct gatgcgcggc gaggtcggtt
    11041 ccaaggtgac gctgaccatc atgcgcgaag gcgagcagca gccgcgccag ttcacgctgg
    11101 cgcgcgacac catcaagctg cccagcatca agagccggct gctcgagtcg ggctatggct
    11161 acctgcgcat cgcccagttt caggccaaca ccggcaccga cctgctgaag gcgctggaga
    11221 agctgcgcaa ggaggccaag ccgctgcggg ggctggtgct cgatctgcgc aacaatcccg
    11281 gcggtctgct cgacgcggcc gtcgcggtgg ccgatgcctt cctgagcgat ggcctgatcg
    11341 tctacaccca ggggcggctg tccgactcca gaatgaccta tggggcgaca ccgcccaatc
    11401 cggccggcga gatgccggtg gtggtgctga ccaatggcgg caccgcctcg gcggccgaga
    11461 tcgttgctgg cgcgctgcaa gaccagcacc gtgccgtgat catgggcacc agcagcttcg
    11521 gcaaggggtc ggtgcagacc attttgccgc tgaccgagga gaccggtgtc aagctgacca
    11581 ccgccctcta ctacaccccg tccggccggt cgatccaggc cgagggcatc cagcccgaca
    11641 tcgtcgtcga gcgtgcccgc atcacccggc tcaaggagga tgatcggtcg atcaaggagc
    11701 aggatctcgc cggccacctg ctcaatggca gccacaagaa tgagccatcc agcagcagga
    11761 aaaagccggc ggagcaggat gagctcgacg gcgactacca gttgcaggag gcgctcaacc
    11821 tgctcaaggg gctcgccatt ctgaaccggc cactcaaggg gggctgatag gccacggtcg
    11881 gctgtcgcga agggtggtgg tcagggcggc tcctgcccct tgagctgggc aatctgccgg
    11941 gccgcgccgg aatgcttcag ccacagccgc tgcgacagca gcgtgccgat ggcaatcagc
    12001 gagagcagga gcagcaggcc aaagaacccc agcagcagcc agagccgggt gcgtggctca
    12061 tcgacgaaca gaaagagcgc ggtcacagtg ccgccaatca gtccggcgac cagcatttgc
    12121 gcggcgcata gcaacagcag tgcgccggcc atgccgagac gcactgcctg cagcagtttc
    12181 agataggcga tcaacccctg ccgttcacag tgcagccagg tggcactcaa caggcgctgt
    12241 tgcagcagca gacgcgccgc gcggccgaaa aagcccagct tcatttgcgg ccacttctga
    12301 gcagaagccc cagcatcagg ccggccagtc cggccaggcc gagtgccgtc caggggcgct
    12361 cggcggtgct ctgctcgacc tgctgctgca atgtctgcgc atcgatgccg agttcggcct
    12421 tcagctcggc caccgtctgc cgcagccgtt cgatgtcccg ccgcagcagt gccttcgcct
    12481 catcggtaat ggtcgaacca ccctgcgcct gcaactgggt gatggcatcg gaaagcgagt
    12541 tcggttcgcg catgggtgtc tcctgctgcc gacccagggt ggatcagaca ggtcgttcaa
    12601 tcggggtcag cgcagctgaa ttaagcaaat cggccgggta aaagcaacgc gccgccggtg
    12661 gttcctggcc tggagcgctg caaggagcgg cagatcagtc atcgccccca tgcgcttcaa
    12721 gcaactcatc catgtcgtcg ctgacgccgt aaccaaggtc gcggctgatg tttcgtacca
    12781 ccgccaggcg ttccagcaag gcgggtcgcg ccgcatcagg cagcacggca atggctgtca
    12841 atgcctggcc gaacatccgc accaatgcat cgaagtagcc ctcgtcctgc aggccaatgt
    12901 cgtggctgaa acccgccgca cgctcacagt agaacaccat cagttccgcc agtccgtcgg
    12961 gttgaccaac cgcttttctg tagtccgcaa tcgccttctt ggccttggcg accgaggtgt
    13021 cctggttctt gaatgcatct ggccagagcc agcggtcgag ggtgctcttg tagggttgca
    13081 gaacatcgtc gccgagcgca aagcgcgcgt gcaggaatgt ctggttctcc ttgctggcgg
    13141 cgtagaggtc atgcaccaga ccggatcagg ccggcacggt cgaagccggc cagcctggct
    13201 ttgacgtcgc tccagctggg cctgtttttc ttcgtggtca tcgtcttgtt cctcgtcgct
    13261 ggccggctcc gttcctcttg ggcggcgggt gatgattttc ttccattcat ccgggatccc
    13321 tgttcatcat gccaacccga agtagcattc aaaaaacgcc gcgagtttgt ccagcacggc
    13381 ctgcttcttc agcccgtggc cgttttccct ggagaagcgt gatcccggcg gcagcacctt
    13441 ggtgatggcg gtgccggtgg tcgggatggc gccgtcatgg aaggcgtttt tcattgtgcc
    13501 ggcgtctgtg cccgccgctc gatctcctcg accagctcgc tgatggcggg cagccgttcg
    13561 gcgttgaggg tgatggtgtc agcgagctca tcggccgtca gtggctcctg cgttttcaac
    13621 tggctctcca gcaccgccag gttggcatcg gagacctcgt tcgggtcgct ggcgcgggcc
    13681 ttgagtcggc tcaccagggt ggcgagttcc gcgctgatcg agaggatgat ccggcgcacg
    13741 ccgagctcgg tggcgatggc ggcaaaagtg gcgcgttgcg gtcgatgcag aaaggtggca
    13801 tcgacgatga cggcaaagcc ggccgcgatc agggtgcggg ccagttcggc cagccgcgcg
    13861 aaggtgcgct gggtggcttc ggcgctgtaa agccccgccg ctgcaccgcc acgctgggtg
    13921 cggtcgaggc catgcagccg tttgcgctcg acatcggagc gcagccgcac ggcgccggtc
    13981 tggctggcga tgtagagcgc caggtgtgac ttgccgctgc cggagaggcc atgggccagt
    14041 cccagccagg ggtgtctggg ctggatgaac tgctcggcca gccgcaggta gtggctgccc
    14101 tgctgcacgg cctgctggcg tgctgcgcct tgcagttgtt cggcgcgcag cagcgccacc
    14161 ttggcgcgca ccagcgcgcg gtagacctgg tagaaattga acagcagcag cccttcatgg
    14221 tcgccgctgg cctcgaggta gccgttcagc aggccagcgg ccagttcggg gtgaccgcgg
    14281 tcggtcagat ccatggtgac gaaggccagc tcgctctgca cgtcgatgaa gcgcagatcg
    14341 gcgttgaatt cgaggcagtc gaacagcacg aactcaccct ggatgcgggc gatgttgccc
    14401 agatgcaggt cgccatggca ttcgcgcacg cagccggccg ccttgcgggc agtgaagtgg
    14461 cggccgagcc gcttgccctc ggcttcgctc cagcgctcca gccgcagcag ccgggcggcg
    14521 tcgaccgggt catcgatgcg cggccgcagc tgggcgaagt tctcccgcac cggcgccatc
    14581 accctggccg cgctgccatg ggccgagtcg gcggcggcct cggcgagcag ttggtgcttt
    14641 tcggcgatca gggcgccgag gcgaaaggcc tcttcgaggc tgagctggcc agccgcgagc
    14701 tgacggtcga gcagggcact ctggtcgaac tggcgcatca ccaccgcatg gtcgagcacc
    14761 tcaccggtgc cgccgatgcg cggtgcctgc ggcgtgccgg tgatcggcac cacccgcagg
    14821 tagagcgaca gcgtgaagcg gcgattgagg cgcagctcct cttcacagaa ccgcttgcgc
    14881 agcgccaggg tcgaaaaatc gacgaagccg aagttgaccg gcttcttgac cttgtaggcc
    14941 agttcgccgg tcaggatcac ccaggagatg tgggtctcga tcagctcgat ccgcccggtc
    15001 gggtgggggt agcaggcggg gttgagcagt tgctcgatcc aatgtgcagc catggcgccc
    15061 ttcgtgttca ctccatgagg cgggcgaaga cccgccgggc ggcagcgaag gtgtgttcca
    15121 gttcggctgc accatgcgcg gtggagagaa agccggcctc gaaggccgag ggtgccagat
    15181 agactccttc gtccagcatg gcgtgaaaga agcggttgaa gcgtgcgctg tcgcagcggg
    15241 tggcctggtc gtagctgctg acctcgggtt gtgtggtgaa gaagaggccg aacatgctgc
    15301 cgacatgctg ggtgatgaag gggatgccgg cgctgcgggc cagttcggcc agcccctggc
    15361 tgagggcgct ggtctgggct tcgagccggg cgatgacgcc gggctgttcg agctgttgca
    15421 gcgtggccag cccggcggcc atcgccagcg gattgcccga cagggtgccg gcctggtaga
    15481 ccggcccgag cggcgccagc ctggccatca cgtcacgccg gccgccgaat gcgccgaccg
    15541 gcaggccgcc accgatcacc ttgccgagca cggtcaggtc cggcgtgatg ccatagcgcg
    15601 cctgggcacc gcccagcgcg acgcgaaagc cggtcatcac ctcgtcaaag atcagcaggg
    15661 cgccatggcg gttgcagagg tgctgcaacc cttcgagaaa gccgggcagc ggcgggatgc
    15721 agttcatgtt gccggccacc ggctcgatga tgacgcaggc gatctcactg ccctgcgcgg
    15781 cgaagagcgc ttcgagctgt gccaggttgt tgtattcgag ggtcagcgtc agttcggcca
    15841 gtgccttggg cacgcccagc gagctgggca cgccgaaggt gagggcaccc gaaccggcct
    15901 tgaccagcag tgagtcgcca tggccatggt aacagccctc gaacttgacg atcttgtcac
    15961 ggccggtgaa gccgcgtgcc agccggattg cgctcatggt ggcctcggtg ccggagctga
    16021 ccatccgcac ctgttcgatc gatggaatca gcgcacggat cttctcggcc agttcggttt
    16081 cgagcgcagt gggggcgccg aagctgaaac ccagccgcag ccgcgactcg acggctgcca
    16141 ccacggccgg atgggcgtgg ccgagaatca gtgggcccca ggagcagaca tagtcgacat
    16201 agcggcggcc atcgacatcg atcagccagg cgccttcgcc gcgttcgatg aagcgtggcg
    16261 tgccaccgac cgagcgaaag gcgcgcaccg gcgagttgac gccgccggga atcacctgct
    16321 gggcggcgct gaagagggtc tctgagtggg tcatggtctg gctgcctgcg gttcgatgaa
    16381 gagtcgttga aactggagag cgcgctgcgc gatgtcgtgg ctgccgaaca gcccttcgat
    16441 cacggcgatg gcatcggcac cggcttcgat cacgctgcgg gcattatcga cgctgatgcc
    16501 gccgatggcc acgatcggca cgtcgagccg tcgacgcgcc tcatgcagca gcccgagcgg
    16561 cgccggcggc gcctgtggct tgctgcgtga ggcaaagaag cggccgaagg caacatagct
    16621 ggcgccggcg gccacggccg tttcggccca gtcgagccgc tgatggcagg tggcgccgat
    16681 cagtgccgtc gggcccagtt ggcgccgtgc ggtggcgaga tcgccatctt cacggcccag
    16741 atggagtcca tcggcgccga tgtcgagcgc cagttgcagg tcgtcgttga cgatcagcgg
    16801 ccgaccctgc ttgcggcaga gcgcgagcag ggcctcggct tgctgccggc ggcgctgcgc
    16861 atcggtcgac ttgtcgcggt actgcaccag ttgggcgccg cctttcaatg cggcttcgac
    16921 ccactgcaac agccgtgcgt cgtcagtggt ggtcggggtg atggcgtaga gtccgcggat
    16981 catcggggcg cgatccgccg gggaatccac tgtccaccgc ccagcggctc tgccagcacc
    17041 atggcgcggt gactctgctc gctgctcaat ctcaccgccg tcggcagtgg ctggcccagc
    17101 gcgatctggg cgcagagcag ggccgccaag gtgcagccgc tgccatggaa gcggtgcggc
    17161 agccggggcc agcggtcacg ctggatcagc ccctgccggc cgtagaggcg gttctccagc
    17221 cagggcgtgt cgccatcatg gccaccactc accagcaggt gctcgcagcc cctgtcgagc
    17281 agccggccga ccgcactggc ttcgtcgtcg accccggcga gctggaacag ttcttcgcgg
    17341 ttgggggtga ggcaggtcgc ccgtggcagc agccgctggc gcagcgcatc gaggtaggcg
    17401 tggtcagtga ccaggtgtcc gccaccgctg cgcaggatcg gatcgaccac cagcggcagc
    17461 cgtgacagtg cggtttccag cagggcggcg acttcgtcga tggcggccgt gctgccaagc
    17521 agaccgatct tgatggcgct gatggcaaag tcgtcgagca ggcaacgggt ctgggcagtg
    17581 atccagccgg ctggcaccgg ctcgatccgc gcgatgtcgt ggctgttctg cacggtcagt
    17641 gccgtcacca cggtcagcgc atggcagcct gccgcggcgg cggcctcggc gtcggcttgg
    17701 atgcctgcgc cgccggtcgg atcgtgaccg ccaaggcaga ggatcagcgg gcgcatggtc
    17761 ggggctggct cagaagggac gcacgatcac cagaatcacc accggcagca ggatcagtac
    17821 cgggaactca ttgaaccagc ggtaaaaaat ctcgccatgg cgattctgat cggccgcgaa
    17881 gcgttgcagc agccggccgc agtataggtg gtaggcaagc aggatcagca ccagtgtcag
    17941 cttggcgtgc atccagtagg cagtgcggta gctctcccag ttgggcagca gcatcgccag
    18001 ccccagagcg accgtcgcca ccatcgaagg ggtcatgatg ccgcgataga gcttgcgctc
    18061 catcaccttg aagcggtcgc ggctcggctg atcttcgctc atgctgtgat agacgaacag
    18121 ccgaggcagg tagaagaggc ccgcaaacca gcagaccacg gcaatcaggt gaaaggcctt
    18181 gagccagagc atcgagcact cccgcgcatt gagttgggcg aaaccggcac cattgtaatg
    18241 cggtgccgct gatctgtcgg cgttgacgca ctcattcccg ggtagccgag ccgctataat
    18301 ctccgccatt cgtcttggct gttctgaaga gatcaaagga gatcgtcgtg gtcaaagtgg
    18361 gcattgtcgg gggcaccggc tacaccggtg tggagctgct gcggctgctg gcgcgccatc
    18421 cggaggtttc ggtcgagctg atcacctcgc gcggcgaggc cggcattcgc gtcgaccaga
    18481 tctatcccaa cctgcgcggc ttctatgact tggcctttgt cgcccccgat cctgccgccc
    18541 tgagcgagct ggatgtggtc ttcttcgcca cccctcacgg cgtggcgatg gagatggcgc
    18601 cagcactggt ggcggccggc gtgcgggtga tcgacctctc cgccgacttt cgcctgaagg
    18661 atccgctgct gttccagaag tggtacggga tgccgcacag cgccaccgac ctgttgcagg
    18721 aggcggtcta tggtctgccg gaatgcaacc gcgcgcagat tcggcaggcg cggctggtgg
    18781 cggtgccggg ctgctatccg acggcgatac agctgggcct gctgccactg ctggaggcga
    18841 aactggtcga cgaacggtcg ctgatcgccg acgccaaatc gggggtgagc ggcgctggcc
    18901 gtcagggcaa gatcgccaac ctgctgtgtg aggttgcgga cagcttcaag gcctatggcg
    18961 ttcctggcca ccgtcatctg cccgagatca ccgctcatct tggtcaatac ctcggctgcg
    19021 ccgcacctgg cctcaccttc gtgccccatc tggcgccgat gattcgcggc attcatgcca
    19081 ccctctatgc gcggctgaag gatccggcag tccagcccga tctgcagcag ctctacgccg
    19141 agcgttaccg cgatgaaccc ttcgtcgatc tgctgcccgc cggagagcat ccggagaccc
    19201 gcagcgtgcg cggcgccaac ctctgccggc tggcgctgca ccgcccgcaa gggggcgaca
    19261 ccctggtcgt gctctcggtg atcgacaact tggtcaaggg ggctgccggg caggcgatcc
    19321 agaacatgaa cctctgttgc gggctgccgg agacgagtgg tctcgacgcg ccggcgctgc
    19381 tgccctgaag cgagcgctct gccgacgaaa tagttgactt taatgcttgg ttttaatagg
    19441 gtgattgccg gtgctttcac aatgcaggtg agtgagcaat gagcagcgta caggtgcagc
    19501 agccggttgc actcgaggtg accgcggccg cggcacgcaa ggtgcgtcgt ctggtcgatg
    19561 aagagggcaa cagccagctc aagttgcggg tgttcgtgac cggcggtggc tgttccggtt
    19621 ttcagtatgg cttcagcttc gatgacgaag cggccgagga tgacacccgg atcgagcgcg
    19681 atggcatcac cgtgctggtc gatccgatga gctttcaata tctggtcggc tcggagattg
    19741 actactccga agggctcgaa gggtcacgtt tcgtggtgcg caatcccaat gcgaccacca
    19801 cctgcggctg cggatcgagc ttcgcggtct gatccgctgg atcagatgtc aggcagatag
    19861 accccgccga gcatgacggg ccggcgggcg ccggtgacgc tgggcaggtt gcccggtcga
    19921 cccagcaacc gctgccgtgc cagccaggcg aaggtggcgg cctcgaccca ctgcggtggc
    19981 agtcccagca gttcggtgga gtgaatccgc cgcccgggca gggcagcctg caaccgatcg
    20041 accagcgcgc ggttctcgac gccccctcca cacaggtaga gttcggtccc gcacccccag
    20101 cggtcgatgg cctgcgccac gctccgggcg gtcagttcga gcagcgtcgc ctgcacatcg
    20161 accggagcgc agtgcgcgaa gtcgcgcagt cgttgcgtca gccagttgag gttgaaccgt
    20221 tcacgaccgg tgctcttcgg cggagttgcc tggaaaaaag cgtcgtccag caaggcgcgc
    20281 agcagcggtt catggcaact gccactgcgg ctccagttgc cacggtgatc atagggctgg
    20341 ccgaggtggc gctgacacca gccatcgagc agcgcattgc ccggcccggt gtcgaagcca
    20401 acgaccgcgg cagggtgctg cgtcggcggc agaaccgtca gattggcgat gccgcccagg
    20461 ttgagcacca tccgatattc agtggggtgg gagaacagcc agtggtggaa tgccggcgcc
    20521 agcggtgccc cctctcctcc ggccaccatg tccttgcggc ggaaatcggc caccgtgacc
    20581 aggccggtca ggtaggcgat gcggttggga tcgcccacct ggcaggtgaa ggcatggtcg
    20641 ccgccgggtg aatggcgcag ggtctggcca tggctgccga ttgcgcggat gcgcgcgcga
    20701 tcgatgccgc actgcgccaa cagttgattg accgccgtcg cgatcagctc ggcatgatcg
    20761 atgtcgagcc gatggaggcg attgagttca tcggggcccg gtgtggcgag cgcgatggcc
    20821 tcctgccgga tgtcggcggg aatcggcagg ctgtgcttcg ccagcagcag acaggaggtg
    20881 tcggtgaacc ggaacaggcc gacatcgacg gcatcaaggc tggtgccgga catgactccc
    20941 agatagcagt cgccgtcgga tctctcttcc gtgctcaatc attcaacccg gcgcgtgcca
    21001 ccaccgcggg ggtggactca ccctgcttca gcatcgccac caaggggccg gtctgtgact
    21061 tgaagcggtc gagctcgcgc ccggccacgg cgggcgcttg cggcagcggg acggtcagcg
    21121 ggttgcggtg cacgccatcg acctggaact catagtgcag gtggttgccg gtggcgagac
    21181 cgctggcgcc gacatagccg atcacttcac cctgccgcac cttctgacca ttgcggatcg
    21241 agggggcgaa cccctggagg tgggcataga gggtggtgta gcggtcacca tgctggatcg
    21301 tgatggtgtt gccgtagcca cccttgcggc cggcgaaagt cacccggcca tcgccgacgc
    21361 tcttgactgg cgtgccggca ctggccgagt agtcgatgcc cttgtgggcg cgcagggtgt
    21421 gcagaatcgg gtggaagcgg ctcaggctga acagtgaggt gatgcgcacg acgtcgagcg
    21481 ggttgcggat gaacgccttg cgcatgccgc ggccatcggg agtgtagtaa ccctcgttgc
    21541 cctgcgcatc gacatggcgc acggtctggt agcggcgtcc ctgattgatg aattcggcgg
    21601 ccagaatcgg accgtcatcg atcttcttgc cgtcgatgaa gcgctcctga tagatgacag
    21661 agaagcggtc gccctggcgc agatcctcgg tgtaatcgat gtcccagccg aagaggtcgg
    21721 ccaactggct ggccacggct tgggagagcc ccgctttctg gcaagagaga aacagcgact
    21781 gatcgatcac gccgctgtgc cggcggagct ggagctcggg ctcgcgcttc tcaacggtga
    21841 catcgaagct gtcgttggtc ctttgccact gtaccgactc aagcgcgttg gggctgaatt
    21901 tgaacctctc cagttgaccc tccgtgccga cttgcagtgc cagtttgtgc cctggatgga
    21961 tctgatgcag ccgcttggtc tccttgcccg aatcgagcag catcgccagt tcgttgggtg
    22021 cgatgccggc gcgtttgaac agcgtgccca gcgtgtcgcc ggtctggacg acatattcat
    22081 cccacagttc cggctcgacc agagcggtgg tgacggccac cgccggctgt tcggtgctct
    22141 gcggtgtggt gggggcgggc agggccagcg gcacgacact cttgatggcg gcggcctctt
    22201 cgcttggcag cagccaggcg atcagcacgg tggcgctcag cagcagggtg gcggcgatca
    22261 ggtgcagtcg agggaaggct gttttcacag cagaagctgg tggcagtgct gccatcggat
    22321 acaatctcac tttatcgttc ttccgttgcg ggccggatta tatcaactga atccggcagg
    22381 gctaccccgt cgagtgggcg gcccggtgcg ttagtgcgac atccgggcca atggttcaat
    22441 gcatctgttc ggtgccgtct gaaaattggc actgaaacaa ggtgtcaacc tcaaataagc
    22501 aatatagaca ccaatttaat gggttgtgac gaatgacatc ggtaaaggcc gcgctcgagc
    22561 tcatcaaggc cggggcgcat gagatcatct ccgagccgga gctgatcgaa aaactgggca
    22621 gtggccgcaa actgcgggtc aaggcgggct tcgatcccac cgcgcccgat ctgcatctgg
    22681 gtcacgcggt gctgctgaac aagctccatc agttccagca gttgggccat gaggtgctgt
    22741 ttctgatcgg tgacttcacc ggcatgatcg gcgaccccac tggcaagaat gtcacgcgca
    22801 agcccctcac ccgtcagcag gtcgaacaga atgcgcagac ctacagcgag caggtgttca
    22861 ccgtgctcga ccgggcaaaa acccaggtgg tgttcaattc gcagtggatg ggcaggttcg
    22921 atgccagtga catgatcaag ctggcgagtc gctacaccgt tgcccgcatg ctcgagcgcg
    22981 atgattttca caaacgctac cgtggcggcc agccgatcgc cctgcatgag tttctctatc
    23041 cgctgattca gggccatgac tcggtcgaac tgcgcgccga catcgagctg ggcggcaccg
    23101 accagaagtt caatctgctg gtcgggcgtg agttgcagcg cgatgccggt cagacgccgc
    23161 aggtggtgat gaccatgccg attctcgagg gattggatgg cgtgcagaag atgtccaagt
    23221 cgctcgacaa ctacattggc ctgcgtgaac cgcccaacac gatgttcggc aaactgatgt
    23281 cgatcagcga caccttgatg tggcgctact atgcgctgct gagcaggcgg ttgccgacag
    23341 aggtcgccgc catgcaggcg cagtgcgagg caggagagct caatccgcgt gatgccaagg
    23401 tggagctggc ccgggagctg gtgacgcggt tccacagtgc cgccgaggcc gaggcggcgc
    23461 atcaggcgtt cgtggcccgc tttcagcagg gtgccctgcc gcaggagatt cccgaactgt
    23521 gcatcgccca tggcggttat ccggcagagg gggtgccgat cgccaacctg ctgaaggagg
    23581 ccggtctggt cgccagcacc tccgaggcgc tgcggctgat cggccagggc ggggtcagga
    23641 tcgatgggat ccgtttgcag gaaaaaaacc atcgggtggc acccggcagc gagcatctct
    23701 atcaggccgg caagcaccgt tttgcccgga ttcgggtgaa ttgatgcgtc ccgcgcaccg
    23761 acacggagcg ctggagcact gttgaggccc catgtacaaa ctggtctttt tcgttccgat
    23821 cgagcagctc gaagcggtga aagcagcgct ctttgccgca ggtggcgggc ggatcggcca
    23881 ctatgactgc tgtgcctggc aggtgctggg gctgggtcaa tttcgtccac gtgccggcag
    23941 caatcctttt ctgggcagcc aggagcagtt ggagcaggtg ccggagtacc gggtcgaaat
    24001 ggtggtggcc gatgcactga tcgagccggt gatccgggcg ctccatgcnn nnnnnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tacaactcaa aggagaactg tcgatggaga
    24181 tgcactttgc caccctgtgg gagcagatcg ctgcccagat tccccagcgc acggcgctga
    24241 tccatggcga gcgcaaggtc gcctggggcg cctaccagga gcgcgccgcg cgcatcgccc
    24301 agtttctgca tcagcagggg ctggggcatg acagcaagat gggactctat ctctacaatt
    24361 cgcctgagta catggaagcg cagttcgctg gcttcaaggt tcgcggcgtt ccggtcaacg
    24421 tcaactaccg ctacctcgag gatgagctgg tctatctgct cgacaactcc gacagcgagg
    24481 cgctgttctt tcatggccgc ttcgccgagc gcatcgccaa cattcggcac aagctgccca
    24541 aggtgcgctg cctgatccag gtggaggatg gcagtggtgc gccgctgctg gccggcgcgc
    24601 acgactatga acagatcatc cgtacccatg cgccgatggc gccgatcgag cgcggctggg
    24661 atgacaccta catgctctat accggtggca ccaccggcat gccgaagggg gtgatgtaca
    24721 agctgggcga gatgtgtgcc ggatttctgg cgccgttcga ggcgcgcggc gtctcgccgc
    24781 ctcccgccac catcgaagag acgctggcgg cgatcaagcg gctggagccg gccggactgc
    24841 tgccggtcac gctggtggcc tgtccgctga tgcacggcac cggcatgtgg cttggcgcga
    24901 tgatgccgca caacatgggc gggaccgtgg tcaccctggc cggcaccaag ttcgatgccg
    24961 atgagctgtg ggcggcggtg cagcgcgaac gggtgaccga catcgtcatc gttggcgacg
    25021 cctttgccaa accgatgctg gatgccctgc gcgctgcaca ggcagcaggc cgtccttatg
    25081 acatctcgtc ggtcaggttc atcagctctt ccggcgtcat gtggacccag gaggtcaagg
    25141 ccgggctgct ggagttcggc gacatgatcc tcaacgatgg gctgggctcc accgagggcg
    25201 gcatgggccg ttcgctgacc acccgcgcca acatcggcga gaccgccaag ttccagatga
    25261 tggagtcgac caaggtgttc gacgacaacg acgaggaggt caagcccggt tccggcgcga
    25321 tcggcatgat cgcccaagcc ggtcgggtgc cgaccggtta ctacaaggat ccggagaaat
    25381 cggccaagac cttccgtacc attcgcgggc tccgctactc gtttcccggc gatttcgcca
    25441 ctgtcgaggc cgatggcacc attcgtctgc tggggcgcgg ctcgatgtgc atcaacacca
    25501 tgggcgagaa ggtctatccc gaagaggtcg aggaggcgat caagacccat ccggcggtct
    25561 atgactgcct ggtggtgggg gtcaaggatc caaaatatgg cgagcgggtg gcggccgtgg
    25621 tctcactgcg tcccgggcag caggccgacc agcagacgct gatcgaccat gtggcacaca
    25681 ggctggctgg ctacaagaag ccccgcaaca tcttcatcgt cgatgaggtg cagcgcgcca
    25741 gcaacggcaa ggccgactac aagtgggccc gcagccgcgc cgacgaagag gcgcgcaaac
    25801 tggagatggg aggctgaatg gtcgcaatgg caccgacggc agcgttccag cccgacttcg
    25861 aggccgccca gtccgagctg ctgaccctgc gcgactttct gcgttggggg gtcagcagtc
    25921 tgggggcagc gcaggtggcc ctgggccatg gctgcgatga tctgtgggat gaggcgttgc
    25981 ggcttattct gcacgcgctg catctgccgc tggaggtcga tgaccgggtg ctggatgccc
    26041 ggctgctgcc gcgcgagcgt cagcaggtgg tcgagctgtt ccggcgccgc atcgtcgatc
    26101 gattgccgaa tgcctacctg accggcgagg cctggtttgc cggcctgcca ttcttcgttg
    26161 atcagcgggt gctgattccg cgctcgccgc tggccgagct gatcgcgcgc cgcttcgagc
    26221 cttggatcga ttccagccgg gtcgatcggg tgctcgacct ctgcaccggc agtggctgca
    26281 tcgccatcgc tctggcccat gcgttcgagg ctgccgaggt ggtggcgacc gatctctcgg
    26341 ccgccgcact cgaggtggcg gccatcaacc gggcccgcca tggcctcgaa cagcggctga
    26401 cgctgatcga aggtgacctc ttttccggag tgaccggcac ctttgatctg atcgtcagca
    26461 acccgcccta tgtggccgag caggagctgg ccgcgctgcc cgctgagtat cgccatgaac
    26521 cgcgactggc gctggcggcg ggggtggagg ggctcgacat cgtcgagcgc attctggcgc
    26581 aggcccggcg ccatctgcgt ccgggcggtc tgctggtggt cgaggtgggc tcggcggccg
    26641 aggcgctggt ggcgcgccat cctgacctcg gcttcttctg gcccgatttc gagcagggtg
    26701 gcgagggaat cttcattctg gaggccgatc agctctgatc ggcctgtcgg ttctctgttt
    26761 cagtcggcca atcagagtgg ttttggctgg aagaaacgag gatccgcggc gcgaatgtcc
    26821 tgcatccgca gtggatagtc acctcgttca cggtcggcaa agtggaattc gagggtcttt
    26881 ctgatcaccg caaaggcgag catttcccac ggaatctcct cgcgcgcgaa cagcttcacc
    26941 tccaggctct ccttgccggc ggcaaagccg ggctcgatca gttcgccgcg aaaaaacagg
    27001 tagatctgat tgatgtgcac gaggttgaag agggtgtaga gatcgccgac ccgtacccgc
    27061 gcattggcct cttcccaggt ttcccgcgcg gccgcctcct gggtggtctc atcgttttcc
    27121 atgaagccgg ccggcagggt ccagaaaccc aggcgcggct cgatcgcgcg tcggcagagc
    27181 agcacctgat cattctgggt gatcaggcag ccggcgatga tcttggggtt ctgataatgg
    27241 atggtgccgc agtcgccgca aacatggcgt tcacggttgt ccgactccgg cactttcagc
    27301 acgatggcct cgccgcagtg ggtgcagaac ttcatgggct gactccggca ggcaatgacg
    27361 gtggaaccag caccaatcta cgcaaccact gccctgaatg aaagcggtgg gcgagcagtg
    27421 atcaggcccg tgcttcgcgg gtgcgaaacg gcgtcaccgt ggcggtgggt tcggcgcgcg
    27481 cctgctggct ggtcgggagg ggttcgccct tcagtgccag attcagcgtg tagagcgagt
    27541 catgcatcat ctgcgagagc ttgatgcagg cggccatcgg ggtcttggcg cgccggcgtt
    27601 ccatgtcgat ctggaactgc aacccgcgca gccgctgctg ccggctttca ggcgcctgtg
    27661 cgatcagctc atcgaccagt tgcaggcgca gggcttcgag tgcctgtgga tcctgctcgg
    27721 cgagctggcg cagttgctcg ggggcgggca gcggagtgaa tgtaggcatg gcagtggctc
    27781 ccggtgaagg caggcaaaca gtgcatgaaa gaaaaatttg cccgcgcgac ttgctaaaac
    27841 tcaaaagcag tctacccggc gcggtcgagc tgatcaatat ttgcacagac tgtggctggc
    27901 ggtggtgcgg caccggaatt ttttggtgtt tttgtcgaaa tatcgctgca aaaagcgtat
    27961 ggatgctggc tgttgagcga agttgtctgc atggatctca atcaatccat ttgcataata
    28021 gaggcatagg caagaacatt ttcttatttg aaagccgaaa tcgtcggttt tttgtcctgt
    28081 cacgcccggt tttggcagaa attccgacgc tgttggcggc gctggcttgc cgccgtctat
    28141 gggtggattg gtttagaatg gcggcagact cgaagcagcc ctgtcccgct tcatccttga
    28201 tcaacccgcc atggagagag aacagtgatc taccgtctgg gtgaacgtca gctcgaagtg
    28261 tgtggcgatg agttctttgt cgccgacagc gccgtggtca tcggcagcgt gcggctcgag
    28321 agaaatgcca gcatctggtt caacgcagtg ctgcggggtg acaacgacct gatcaccatc
    28381 ggcgaagatt ccaacatcca ggatggctcg gtgctgcaca ccgaccatgg ggtgccgctg
    28441 accgtgggtc ggggggtcac cgtcggtcac aaggtgatgc tgcatggctg caccatcggt
    28501 gattacagtc tgatcggcat gaacgcagtg gtgctgaacg gcgcccgcat cggaaaatat
    28561 tgtctgatcg gcgccaacac gctgattccg gagggcaagg agattcccga cggctcgctg
    28621 gtgatgggtt cgccgggcaa ggtggtacgc gagatctctg atttgcagag aaagattctc
    28681 gagggcagcg cccaacacta tgtcgaaaat tccaggcgct acacgcagca gttgcggctc
    28741 gatccgcgct ttcatggctg atccgcagat catgggtgag cggccgccgc cgtcgccctg
    28801 tgtgtcggtc tgtgcgctgg atgaaatgga catctgcatc ggctgtcacc gctccgtgga
    28861 tgagattgcg gcgtggtcgc agctcagcgc tgcgcagaag tggcaggtca ttcaattgag
    28921 tcaggcacgc gtccgggctg cgggtggctg gctgattccc ccggatggag cagaaccgtg
    28981 acagagatag gtaccccgaa ctccccgacc gcgctgcggg tgctgctgct gggcagcggt
    29041 gaactcggca aggaggtggc gatcgagctg caacggctcg gtgtcgaggt gatcgctgtc
    29101 gaccgctatg ccgatgcgcc ggcgatgcag gtggcgcatc gcgcccatgt catctcgatg
    29161 ctcgatggcg ctgcgctgcg gcgggtggtc gagctggagc agccccacta catcgttccg
    29221 gaaatcgagg cgatcgccac cgatgagctg ctgaaactcg aggccgaagg ccatcgggtg
    29281 gtgcccaccg cgcgggcggc acggttgacg atggaccggg agggcattcg ccggctggct
    29341 gccgaagagc tgggcctgcc cacctcacct tacggctttg ccgacagcga ggcggagctg
    29401 atcgagcagg tcgcgcgaat cggctttccc tgcgtggtca agccggtgat gagctcctcc
    29461 ggcaaggggc agtcggtggc gcatgaccag gccgggctca tcaaggcgtg gcgctatgcc
    29521 caggagggcg gtcgggccgg tgccggacgg gtcatcatcg aaggcttcgt ccggttcgac
    29581 tatgagatca ccctgctgac catccgtcac cgcgacggca ccaccttctg tgcgccgatc
    29641 ggccaccgac aggaggcggg cgactaccgt gaatcctggc aaccacagcg gatgagccgg
    29701 gctgcgctcg aacgctccga agcggtggcc gccaaggtga ccgaagcgct gggcggcttt
    29761 ggcctgttcg gtgtcgagct gttcatctgc ggcgatgagg tctacttcag cgaggtgtcg
    29821 ccccgccccc atgacactgg catggtgacg atgatctcgc aggatctctc cgaattcgcc
    29881 ctgcatgcac gcgccctgct ggggttgccg attccgaatg tgcgccagct ggggcctgcc
    29941 gcctcggcgg tgattctggt gcagggggcg tcgcaacagc cgagctatgg tgccctggag
    30001 gtcgctctgg ccgagcccga tacccagttg cgcctgttcg gcaagcccga tgtggccgga
    30061 cagcggcgca tgggcgtcgg cctggcacgg gccgacagcg tcgaagcggc gctggaaaag
    30121 gccaagcggg tggcagccgc ggtcagggtc aggctgtaaa tcgatgctgt cccactcaat
    30181 aagatcacca aggagactcg catgaagccg accctctatg gcgctccact gtcacccttt
    30241 gtgcgcaagg cacgcatcgt gctggccgaa aagggcatcg actacgatct ggtgccgctc
    30301 gatccgtttc gcaagagtga ggagctgctg cgactcagtc cgctcggacg ggtcccggcg
    30361 ctggtcgacg atggaaaaac cctggccgac tccggcgtca ttgcccaata cctcgaaaag
    30421 aagtggccga cgccgccgct ctatgccgat gacccctacc tgcatgcacg ggtggtctgg
    30481 ttcgagaagt acgccgacta cgaactggtg ccactgtgca ccttttcggt ttttcgccag
    30541 cgcctgctga tgccgatgat gggacagggt tgcgatgagc agctggtaca gagcgcgctg
    30601 cagaagttgc cggcccactt cgactacctg gagcagcagc tcgacggcgc cgaccatctg
    30661 atcgacaacc gtctcaccgt ggccgacatc gcggtggctt cgcagtgcat caacatgggg
    30721 catggcggcg aatgtgtcga tgcccagcgc tggccgacgc tggcagcact ggtcgagcgg
    30781 gtgaaggcac gccccagtgc cgcggactgc ctggcgcaag aggcggcgat catcgacaag
    30841 attcgcgctc gcacggctgg cggctgagct gcatcaaccc ggcgatgttt caatggctgt
    30901 tcgcccggaa gttcctggag cgctgatcgg atcgccgctg gggttgggtc tgccagttcg
    30961 agacggcatc gatctgcatg agatgctccg tcatcggtcg gaccgcgatg ggagggccga
    31021 ccgtgtcgtt tggtctctct gcgcgatgga tgtccgttga agacccgtta attggaaaaa
    31081 tcagccagtt ggccggcagg cgccttctgg tttgggatcg agtttcgttt cgtcgggaag
    31141 ggaggaaaaa gagggcggtc gattcccggg agggcgaaca gatcaagggg cgcccggtcg
    31201 cgccattggt acagggacca tcacggagcc aaacgtttca gggaccaggt ttgatccggg
    31261 cggcgctgat acagcaagcg gtcgtgcagt cggtcgattc gcgcctgcca gaattcaaaa
    31321 cgcaacggaa gcagccggta tccgccccag cccggcggcc gcggaatctc cctgtcggcg
    31381 aaatcgcggg tgaagcgatc gattctcgcg tccagctcct cgcgtccttg cagcacccgg
    31441 ctttgcggcg acgcccaggc gccgatctga ctgccctgtg ggcgggtgcg gaagtaggcg
    31501 tccgattcgg cgggatcgat ccgttcgacg ccgccttcga tgcgcacctg ccgaagctga
    31561 accggccagt agaacagcag ggccgcagag gggttgtggg agagttccgc acccttgcgg
    31621 ctttcataat gcgtatggaa gaccagccca tcttcatcga actgcttgag cagcacgatt
    31681 cgcgcgctgg ggcgaccatc gacaccgacg gtcgccaagg tcatggcgtc cggttcgggt
    31741 ggatgcaggg gccgggcagc atcataccat tcacggaaca atccccacgg gtggggcggc
    31801 gcttcgtgtt cattcaaggg agggaatagg gtggtcatgg tcaagatggc tgctccagac
    31861 aagaaaatgg ggacagtctg atctatccca agatagctca ggcccggatc atttccctca
    31921 tcaggatagt ctggtgagct gtccgatctg aacagggtgt tccatggtcc gcctgtcgcg
    31981 acctttgtgc ttggccaacc gctgtatgcg atccagcgca gcaaccgccg cgaacccacc
    32041 ctccaggagc ggttcatcga ttgccatgca acactgctct gcatcaaccc ggcctgcttc
    32101 aactcaaagc tcgccggctg gcttgtcatc tgccacgctt tcatcttaaa tcgttgctcg
    32161 gcctgatctc cccggtaaac tggcgcctcc agaaccaccc ggtgccgaag gtgctggagc
    32221 agtgcgactc tttgacacgt ctgcttgcgg catttagaat ctcgccccct tatgtcatct
    32281 gatttgctgc ctcttcgggt cgatccgttc aagttggtca aaagcggtgc tgtgctcgag
    32341 ggacggctcc agatggggcg ctttcgccgg ttctgtgccg agctgaccgg cgatgaaggt
    32401 cagctcttcg tccagttgca ggccggacgt gatgagcagg gggttgcctg cctggcgggc
    32461 cgtctgaccg gcgaggtggg gctgatctgt cagcgctgtc tggagccgat gcgccacccg
    32521 atcgatgtgc cgtttcggtt ggcactgctg cagagcgagg cgtcgatcga gcggttgccc
    32581 gacagttacg atccgctggt gctgccggca gaggccggcg ccgtcgagtg gccggtgctg
    32641 ctcgaggatg agctgctgtt gagcttgccg atcattcccc gccatccgtg ggcggagtgt
    32701 tcggcgcccg gggagtactt tgctgcggcc gatcggaccg atccggcgca atgaattttg
    32761 agtgagacat tgattccgtc attgaacgag agaacagaag atggccgtac aacagaaccg
    32821 tgtgacccgc tccaagcgtg acaagagacg ctcgcatgat gccctgacgc cgccgacgct
    32881 gtcggtcgac agtgtcaccg gcgagaccca tcgccgccac catgtcagcg ccaatggcta
    32941 ctaccgtgga cgcaagatcg tctcgggcaa ggacgacgat tgatgtctga ccatgcggac
    33001 cgtcgccatt gatgtgatgg gcggggacca cggtcccgcc gtcacggttc cggccgcgct
    33061 tcagtcgctc tcccgcgatc cgctgctcca tctgctgctg gtcgggcggc aggagcagat
    33121 cactccgttt ttgcgcgatg ttccggctgc gctgcacgag cggctgcaga ccgtggatgc
    33181 ccgtgacgtg gtggccatgg atgagcgccc gtcggtggcg ctgcgcagca gaaaaccctc
    33241 gtccatgtgg ttggccatcg aatgtgtgca gtccggtcgg gcgcaggcct gtgtcagtgc
    33301 cggcaacacc ggcgcactga tggtgatcgg tcgtcatctg ctgaagatgg tggccggcat
    33361 cgaccgtccc gccatcatca cccagattcc gaccg
//
LOCUS       DAORCQ010000017        33269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_694_length_33269_cov_5.663576, whole genome shotgun sequence.
ACCESSION   DAORCQ010000017 DAORCQ010000000
VERSION     DAORCQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 33269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33269
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_694_length_33269_cov_5.663576"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKI23_02985"
     CDS             complement(<1..255)
                     /locus_tag="PKI23_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNN35938.1"
                     /translation="MTMFETLLGFVIVLTVIVTVHEFGHFWVARRMGVRVLRFSIGFG
                     APLYAWTSRAGTEFVLAAIPLGGYVKMLDEREGEVAPAQQH"
     gene            complement(304..1485)
                     /gene="ispC"
                     /locus_tag="PKI23_02990"
     CDS             complement(304..1485)
                     /gene="ispC"
                     /locus_tag="PKI23_02990"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNN35939.1"
                     /translation="MQRITILGSTGSIGTRTLDVIALHPQRYQVFALTAHRRVDLLFA
                     QCRQFNPLYAVVADERLADALISRLRAAELATEVLWGAEGLCQVAAESRVDTVMAAIV
                     GAAGLLPTLAAVRSGKRVLLSNKESLVMSGELFMEAVADSGAQLLPIDSEHNAIFQCI
                     AGCSKKIFDERVIDRIILTASGGPFRTLPIDQLQSVTPEQACAHPNWEMGRKISVDSA
                     SMMNKGLELIEACWLFDATPDQVDIVIHPQSIIHSMVQYVDGSTLAQLGNPDMRTPIA
                     HALAWPERIVSGVPALDLVAIGQLEFSKPDLARYPALALAIAAARAGGGAGAVLNAAN
                     EVAVDAFLQRRLLFTEMVALLEQVLEQLVPLRVDSIEQVLEVDMRARQLANEWLRRRL
                     K"
     gene            complement(1485..2315)
                     /locus_tag="PKI23_02995"
     CDS             complement(1485..2315)
                     /locus_tag="PKI23_02995"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNN35940.1"
                     /translation="MLRQRILTGILLGALLLGLILATSFKQFALITALITLAASWEFF
                     NLASIERLAVRGLCVGVMLLLVGGSYWLIDTDLDLLRLPLALSAIVWLLPIPWLVRYP
                     EASALWQAPLMRTLWGWWSLMATWLALAALKKLPNGEWHLLYFFALVAAADIGAFAIG
                     RTWGQTKLAPRVSPGKTWEGFAGGLGATLALAAGIALAQGLSLQRSLLAMGIAAVTML
                     FSVVGDLSISMLKRYRGVKDSSHLLPGHGGLLDRLDSICAAAPWFAWVMLCSSQQVDG
                     "
     gene            complement(2309..3085)
                     /gene="uppS"
                     /locus_tag="PKI23_03000"
     CDS             complement(2309..3085)
                     /gene="uppS"
                     /locus_tag="PKI23_03000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNN35941.1"
                     /translation="MAQTVLPSGEDLLPSSRPRHIAIIMDGNSRWAKRRGLSPKAGHR
                     AGIESVRSVVEYCVEQQIEVLTLFAFSTENWQRPAGEVRALMALFLAVLKYEVSRLHQ
                     NGVRLRVIGDRDRFHPSIRRHIERAERLTADNAKSTLVIAADYGGRWDMVQAARRIAM
                     DAMAGLIDPARIDEETVQRYVCLADLPPPDLYIRTAGEYRISNFLLWQAAYSEFYFSE
                     AFWPDFQKEELARAIDSYMERERRFGRRDQESPSQEQTGC"
     gene            complement(3187..3744)
                     /gene="frr"
                     /locus_tag="PKI23_03005"
     CDS             complement(3187..3744)
                     /gene="frr"
                     /locus_tag="PKI23_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNN35942.1"
                     /translation="MINEIRQDASERMSKAVDALGVAFSKIRTGRPSPGLLDGVKVNY
                     YGTDTPLKQVASVSVDDSRNLIVSPWEKKILPAIEKAILSAGLGLNPTSSGELIRIPM
                     PALTEETRKNHIRQARNEAEQARVAIRNIRRDANGDFKELLKEKEISEDDLRRAEEEI
                     QKLTDRFIKEVDALLATKETDLMEI"
     gene            complement(3772..4491)
                     /gene="pyrH"
                     /locus_tag="PKI23_03010"
     CDS             complement(3772..4491)
                     /gene="pyrH"
                     /locus_tag="PKI23_03010"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNN35943.1"
                     /translation="MAATDRKYKRILLKLSGEALTGDQSFGIDPKVLDRMALEIGQLV
                     GIGVQVGLVVGGGNLFRGAQLHEVGLDRVTGDHMGMLATVMNSLALRDALERSDISSR
                     VMSAIPMSGVVEHYDRREAIRYLEQGDVLIFAAGTGNPFFTTDSAACLRGIEINAELV
                     LKATKVDGVYSADPFTDSSAIKYDRLTYQEVLDRKLGVMDLTAICLCQDHGMPLRVFR
                     MDRAGALLNIVVGGSEGTQIE"
     gene            complement(4512..5381)
                     /gene="tsf"
                     /locus_tag="PKI23_03015"
     CDS             complement(4512..5381)
                     /gene="tsf"
                     /locus_tag="PKI23_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNN35944.1"
                     /translation="MTAISASQVKELRERTGLGMMECKRALQEANGDIEKAIDELRKS
                     SGMKAAKKAGRIAAEGVIVTRLAEDGSYGVMAEINSETDFVARDENFLNFSRQVVDRL
                     FSAKTVDVDALMGSELGAVREALVQKIGENISVRRAELKQADSAIVGSYVHGNNKIGV
                     LVVLKNGSSALARDIAMHVAASNPLVVRSTDVSADVIAKEREIYSAQAAESGKPAEIV
                     EKMIDGRIRKFLAEVSLVEQPFVKDPDVKVGDLVKKAGAEILSFTRYEVGEGIVKEQV
                     DFAAEVAAQAGLS"
     gene            complement(5522..6265)
                     /gene="rpsB"
                     /locus_tag="PKI23_03020"
     CDS             complement(5522..6265)
                     /gene="rpsB"
                     /locus_tag="PKI23_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNN35945.1"
                     /translation="MPHVSMKEMLAAGVHFGHQTRYWNPKMGPYIYGARNKIHIINLE
                     YTLPAFNRALGLLGDLAASNSKILFVGTKRSASKIIKEQAERCGAPYVCHRWLGGTLT
                     NYKTIRSSIRRYQEIEKQSQDGTFDKITKKEALMRMRTLEKLERSIGGIKEMNGLPDA
                     MFVVDVDHEHIAITEARKLGIPVIAIVDTNSNPEGIDYVIPGNDDAIRAIQLYVTAVA
                     DAIIDGKERARGVVRQDEFVEVQDESASV"
     gene            complement(6545..7060)
                     /locus_tag="PKI23_03025"
     CDS             complement(6545..7060)
                     /locus_tag="PKI23_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HNN35946.1"
                     /translation="MSTAPANPPPMIPLHGLTEAIHISESEVPFVDTGDGGSMQLLQV
                     DLNQGLWILRTRMPPGRTITKHYHTGSVFAVTLKGRWFYKEYPSYVNAPGSYLYEPAG
                     SVHTLMTPKDQEGITDVWFAIYGANINIDEAGNVVGMVDAAAILRTYRMLCAAQGLSA
                     DKVIVLGEPNS"
     gene            complement(7175..8296)
                     /locus_tag="PKI23_03030"
     CDS             complement(7175..8296)
                     /locus_tag="PKI23_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN35947.1"
                     /translation="MRISNILNLGIKELRSLSRDPTMLVLIVFAFTFAIYSAATAMPE
                     SLNRAAISIVDEDRSTLSTRIVGAFYPPHFMPPVLINQTQMDARMNAGLDTFALDIPP
                     DFERDLLAGRRPTIQLNVDATRMSQAFAGSGYIQSMVNSEVAEFVQRHRSNSVLPVEL
                     TLRARFNPELNKAWFGSVMEVINNVTMLSIVLTGAALIREREHGTIEHLLVMPVTPFE
                     IMTSKVWAMGLVVLVACTLSLLGVVQGLLAVPIEGSLLLFLAGATLHLFATTSMGIFL
                     GTLARSMPQFGLLLMLVLLPLQMLSGGVTPRESMPELVQWIMALAPTTHFVAFSQAIL
                     YRGAGIGVVWPHFAALGLIGAALFGLSLARFRKAISLMA"
     gene            complement(8298..11063)
                     /gene="rbbA"
                     /locus_tag="PKI23_03035"
     CDS             complement(8298..11063)
                     /gene="rbbA"
                     /locus_tag="PKI23_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HNN35948.1"
                     /translation="MSDSAADPTLTPVVRLRDVALHHGAVRALEAVTLELPRGQRVGL
                     IGPDGVGKSSLLALIAGAREIQTGQIEVLDGDMAQRRHRLAVCPRIAYMPQGLGRNLY
                     ATLSVFENIDFFGRLFGHGDGERRRRIDELLISTGLAPFRDRPAGKLSGGMKQKLGLC
                     CALIHDPDLLILDEPTTGVDPLSRVQFWELIDRIRSHRAGMSVLVATAYMEEAARFDW
                     LVAMDGGTVLASGTPAQLLAQSGSETLEQAFIALLPELRRREHRPVVIPPRQPDGDGD
                     AAIEASGLTMRFGDFVAVDNVSFRIARGEIFGFLGSNGCGKTTTMKMLTGLLPASEGH
                     AWLFGQPVDPKAIDTRRRVGYMSQGFSLYAELTVRQNLLLHARLFGVAPAQVARRVLE
                     MGQRFGLLEIMEMLPMDLPLGQRQRLSLAVAMIHQPEMLILDEPTSGVDPIARDAFWQ
                     IMVDLARNDQVTIFVSTHFMNEAERCDRISLMHAGKVLASDSPVRLVAERGVGSLEEA
                     FISHLREVVDEEPPHAVVEVANDVASGSASATTPIRRRRFDPERMFSYSRREALELRR
                     DPIRLTLALLGSLLLMLVMGYGISMDVEDLTFAVLDRDQSTLSRDYVSNLSGSRYFIE
                     RHPIADHAELDRRMRSGEISLALEIPPGFAADLQRGRPVEIGAWIDGAMPQRAETVQN
                     YVRGMHLKWLADLQLRRSGQSGSQGLVNIELRFRYNPDVKSLVAMVPAVIPLLLMMIP
                     AMLTALSVVREKELGSIINLYVTPVTRMEFLLGKQLPYVALAMLNFLMLTLLAVTLFG
                     VPLKGSFLTLAAAALLYVCTATAIGLLISSFMRSQIAAIFGTALLSLLPAIQFSGMID
                     PVSSLEGVGRLIGQIYPTTHFLIVARGVFSKALGFADLQTEMMALLLATPVIFTFCVL
                     LLKKQER"
     gene            complement(11060..12130)
                     /locus_tag="PKI23_03040"
     CDS             complement(11060..12130)
                     /locus_tag="PKI23_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNN35949.1"
                     /translation="MKSRLGGWLKAGALIALVAIGYLVWQRLQPTPLGAGFASGNGRI
                     EAVEVDVAAKVPGRVQAILVDEGDFVTAGQQVAQMDTEVLTAQRREAEARLRQAESAV
                     ATARSQAAQRESEKKAAEAVVAQRMAELDVARKRLKRAELLAAKGVVTAQDLDDGRAR
                     AASAEAALNGAKAQVVAMEAAIVTAQAQVTGALAAVEAGRATIERLQADIDDSALKAP
                     RDGRVQYRIAQPGEVVGAGSRLLNVLDLTDVYMTFFLPTAAAGRLAIGSEVRLRLDAA
                     PEYVIPAQISFVADVAQFTPKTVETASEREKLMFRVKARIAPELLKQHLRSIKTGLPG
                     MAYVRIDPQAQWPEELQVKLPQ"
     gene            12305..13000
                     /locus_tag="PKI23_03045"
     CDS             12305..13000
                     /locus_tag="PKI23_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNN35950.1"
                     /translation="MDKLHYQIQDSVCTIHLDDGKVNAMNLVFFEELHAALDQAEKFR
                     CRAVVIDGREGVFSAGLDLKYMPTLEPAERARFSQTFARAILRLYTLPIPSVAAIAGH
                     AIAGGAILAFACDCRYAIDGHYLIQLNEIKNRMALPNWMSLIASTVAAPATLTQLALH
                     ARAYRPREAFAAQLLTGLAGDGNELNELLQQCCRDFFEFDPASYATTKRWMRNGAAEQ
                     ALSHLKDVQATLM"
     gene            complement(13042..14109)
                     /locus_tag="PKI23_03050"
     CDS             complement(13042..14109)
                     /locus_tag="PKI23_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN35951.1"
                     /translation="MTNRLRLPLNRLLLALLLGAFLAGCDSAGHSAESPSHHLPGGGF
                     RNLPQSPPTGSWWRKLPFIVQFPFQRADMSQLPADHRWSAPQVQAGLDRHAQGDSVTW
                     IGHMTALVRMGGVTLLTDPWFSDHCSPLPPFGPRRVVPPALPVESLPRIDLLLISHSH
                     YDHFDTATLRQLARRQNPLVIVPLGLGELVREQGLTQVVELDWHQSSEFSGLRITALP
                     VIHWSKRTLFKTNDTLWAGFSIVSATGKRLYFGGDAEYGPHYRQLGEQYGPFDLALLS
                     IGAFLPREVMAGHHCEPADCLRLGLDLRARRMAAMHWGTVQLGFDGLMEAPTRFRAAA
                     TQQGVAAERIWIPHIGETQPF"
     gene            14169..14756
                     /locus_tag="PKI23_03055"
     CDS             14169..14756
                     /locus_tag="PKI23_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNN35952.1"
                     /translation="MQLKDRAQGYGWISWLLHWPSALLIVGLFALGLYMRGLDYYHPW
                     YHRAPALHKQIGILLATITLLRLCWRLFNRTPPFEAMLAPWERIVATLAHQLLYLLLF
                     LTLATGYLIATGGGQAVSLTDMLSIPSLVRLHDSDTEELVGELHELFSFLLIGLTLLH
                     ALAACKHHWIDRNRTLLRMLWPGSTSPTAPQEREP"
     gene            14753..15340
                     /locus_tag="PKI23_03060"
     CDS             14753..15340
                     /locus_tag="PKI23_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNN35953.1"
                     /translation="MKRPFHRRLPLAATVAGLLLSPIGQAADYLIDTKGSHAFIQFRI
                     QHLGYSWLYGRFNSFDGRFSWDEKQPTASKVEVNIDPASIDSNHVERDNHLRSKDFLD
                     VKQFPTAHFVSTSVAAEAGGGLLLKGKLSLHGVTRDIDIKMHKLGEGADPWGGYRAGF
                     AGTTTLRLKDYGIQRDLGPASQEVELTLSIEGVRQ"
     gene            15449..15784
                     /locus_tag="PKI23_03065"
     CDS             15449..15784
                     /locus_tag="PKI23_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HNN35954.1"
                     /translation="MAERQLYKVIFTNQGKVYEIYARQIYQSELYGFIEIEELVFGST
                     TQLVVDPSEERLRSEFDSVTRSYIPIHSVVRIDEVEREGVAKISESTGGSNVTPFPLP
                     AFRPGGHKE"
     gene            complement(15803..17086)
                     /locus_tag="PKI23_03070"
     CDS             complement(15803..17086)
                     /locus_tag="PKI23_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNN35955.1"
                     /translation="MSLLQLPEEQLLAREQVLSHAYTQWCAAGLKLDLTRGKPSPAQL
                     DLSDALLGPLEGGHLAEDGTDARNYGGLDGLPEAKRLGAALLEVGSDEVLVGGNSSLE
                     LMYRVLFFSHFFGLRPDATPWREQGGVKFLCPVPGYDRHFSICQHLGIEMVPVALTGQ
                     GPDMAQVEALIGQDPSIKGIWCVPKYANPTGETYSDETVARIAALGRIAPKDFVVMWD
                     NAYTVHDLHTPGDALANLMELARRLGTQTSIFITGSTSKITHAGAGISFLASAPANLA
                     RFRAHLGMSTIGPDKVNQLRHVRFLKDESTLRALMRQHAEILRPKFDRVLQRLEAALG
                     GKEIASWTSPRGGYFISFNTLPGLASEVVGLCRAAGVALTPAGASFPYGRDPQDSNIR
                     LAPSFPSLEEIDRSMEVFVLCVELATLRQRLASRA"
     gene            complement(17233..18333)
                     /locus_tag="PKI23_03075"
     CDS             complement(17233..18333)
                     /locus_tag="PKI23_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNN35956.1"
                     /translation="MTVPTEKLHKALARCGLGSRRALEQLIAEGQVTLNGRVARLGDR
                     VTASDKVTLAGRPVTIRPPVKQQTRLLIYHKPEGEICTRSDPEGRPTVFSNLPKLPYG
                     RWIAVGRLDLNSSGLMLFTNEGELANRLMHPSTAIDREYLVRVFGELPEGVRERLLTG
                     VQLEDGPARFTDLGDGGGQGINRWFYVTVMEGRNRLVRRMWESQGIRVSRLKRVRFGP
                     IFLPSAVKQGRYQELERPQIEALCRYANIPLPADFVADEATAERTRKPAGRGRGMVGR
                     ERTDAPAAPRFGASLRRSPRSRTADETRETAPARGRGSASSPAAAAQRAAERPPRRRT
                     PDDALSNRPQTARKQPARRPAGGKSAPTRPRR"
     gene            complement(18405..18935)
                     /gene="scpB"
                     /locus_tag="PKI23_03080"
                     /pseudo
     CDS             complement(18405..18935)
                     /gene="scpB"
                     /locus_tag="PKI23_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            complement(18932..19738)
                     /locus_tag="PKI23_03085"
     CDS             complement(18932..19738)
                     /locus_tag="PKI23_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNN35957.1"
                     /translation="MPLPVPLQQELPLARVQGEALFRLPEGLYIPPDALEIFLETFEG
                     PLDLLLYLIKRQNLDILDIPVAAITRQYMAYVELMQDSRFELAAEYLVMAATLAEIKS
                     RLLLPKPASTDEESEDPRSELIRRLRDYEQFRQAGERLDQLPRLGRDLALLNCALPPL
                     VRAPLPPSLQLDELLQALRGLLERARLFEHHQVSRELLSTRERMSAILARLSQSHDGF
                     LPFTLFFTVEEGRLGVVVTFLAMMELVKEGLVTLLQNAPLAPIHLQAKRA"
     gene            complement(19769..20980)
                     /locus_tag="PKI23_03090"
     CDS             complement(19769..20980)
                     /locus_tag="PKI23_03090"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNN35958.1"
                     /translation="MSIMESRRRVLSGMRPTGKLHLGHYGNVRTWARLQQEYECYFFV
                     ADWHALTTHYESEMDLEQNALDMVIDWLAAGVNPSLATLFVQSQVPEHAELHLLLSMI
                     TPLGWLERVPSYKDQQEQLKEQDLATYGFLGYPLLQSADILAYRAGVVPVGADQVAHV
                     ELTREVARRFNHLYGGEQGFRENAEAAAARMGKKLAKLYVELRRAYQEQGDGDALERA
                     RILVREQPNLSLADRERLMGYLEGDAKVILPEPQALLTPIPKMPGLDGRKMSKSYGNT
                     LALRDPPELIEKKILTMQTDPARVRRSDPGDPDKCPVWQFHQIYSDGVQQAWVREGCS
                     SAGIGCVDCKRPIIDSIQGELRPLQLKASEYEQDLPLVRNILKEGNARAREEARATLD
                     EVREVMGLRYR"
     gene            complement(21049..21684)
                     /locus_tag="PKI23_03095"
     CDS             complement(21049..21684)
                     /locus_tag="PKI23_03095"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNN35959.1"
                     /translation="MSQFFQIHPQNPQPRLLQQAVAILRKEGVIAYPTDSAYALGCRL
                     ENKAGADRIRAIRRLDERHNFTLVCRDLTEISTYAKLDNIRFRLLKRYTPGAYTFILP
                     ATGEVPRRLQHPKRKTIGIRVPEHRIVQALLEELGEPLMSTSLILPGESEPMTDPHEI
                     RQLLQHQLDLVIDGGFSGLEPSTVVDLTGEAPQVVRLGKGDPAPFADMQAD"
     gene            complement(21681..22541)
                     /locus_tag="PKI23_03100"
     CDS             complement(21681..22541)
                     /locus_tag="PKI23_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNN35960.1"
                     /translation="MKVDLHSHSTASDGALSPTALVERAHRAGVTLLALTDHDTVAGV
                     AEATAAAEQHGEAIRVVPGIEISCHWGRCALHLIGLGIDIDHEPLLRLIEQLRRGRLR
                     RAEILADHLRQRGIEGALLGATALAGSAAPGRVHFANWLVASGHVDTFQQAFDRHIGN
                     EKWQEEVTHWPHLGDAIAVVKAAGGVAVLAHPHHYRLGRLQRLALVSDFSGWGGEALE
                     LGMAGQSPDAQRLWSALARQHRLGGSVGSDFHHERTPWADLGRSLPLPVDIAPIWEHQ
                     RLRQPYGVCA"
     gene            22677..23228
                     /locus_tag="PKI23_03105"
     CDS             22677..23228
                     /locus_tag="PKI23_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNN35961.1"
                     /translation="MKQLLEFIPLLAFFAAYKLYDIYIATAVLIIATSLHYGILWLVE
                     RKLDKTQRWTWIGVVLFGGLTLALGDDTYIKWKAPVLDWLFALAFLGSQLFGEKSLVE
                     RLMGNAVSLPKTIWNRLNLSWVIFFAATGFANLYVVFYHPEFWVDFKVFGSLGLTLLF
                     MVGQAIYLVRHITPEAVEEQGKQ"
     gene            23231..23530
                     /locus_tag="PKI23_03110"
     CDS             23231..23530
                     /locus_tag="PKI23_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNN35962.1"
                     /translation="MLYAIIAEDVPHSLPLRQTARPAHLARLQALQQQGRLLLAGPHP
                     AIDHPDPGEAGFSGSLIIAEFDSLAAAQQWAQADPYVSAGVFAHVTVKPFKQTLP"
     gene            23682..24302
                     /locus_tag="PKI23_03115"
     CDS             23682..24302
                     /locus_tag="PKI23_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HNN35963.1"
                     /translation="MLTLLLATASHAETRYVTDQWRFELRSGNSNEHRIIDYLGSGTA
                     VEVLEPVSNGFIHISTAKGVEGWMRESYLQTALPATLRLEQLTNSATAAAVAAGKLPA
                     TRPLAPSARETELETANQSLTQQLTEAKALCENAAQLDQRNRELLQENQLLQDQSTLL
                     KVESERLREQLEKKEFYLGAGAILLGLLLGVILPHLKPKRKYSEWR"
     gene            complement(24354..26489)
                     /locus_tag="PKI23_03120"
     CDS             complement(24354..26489)
                     /locus_tag="PKI23_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN35964.1"
                     /translation="MLETAVLRSSAWPWLRLPVLPQLLLHLQRRKLDGAVDAQTQWTR
                     LLESDVVLSARVLALQGRAPTAESSDDRLGDCVAALGQWRIEALLDQAVTSQFFSVFN
                     QLGDAGWLDLWARITLRRTFAVELANHLAYPELDDLRLGTLLLGLMELRELAATIDAD
                     FATASALEDPDLERSRQLDLLTTLLGVPRRVVDALHFLDEPLSRTRNAHPLIRLLRAA
                     VDAYPVAITPLLPAQSVGLDEIAANLALPSEELRHANRKAIEAVTVVGQRLELPAAFL
                     TRLAESPDWSGWPLSSSCWGASDDAVALSLAATLRDQALVKDIVTPWAWQPDADLQTL
                     ASAVSQELRLLAGVERFMLYLCTDGSLELLCPAAHDDGMLSDEITLAAGHGDSMVAHC
                     LAQGVALGSWQWEAPLALLDRQLIRELDATAIFCLPLMQNATAGALLVIGTEISTRDR
                     LEKRLPALRYYGLLLGRRLGAIRMHQAQTVKQRQALRGQTSLLLRELRHELGSPLSIA
                     RNYLSIIALRFQHSGLTRTDLLPVEAEIDRVVELVDSIVQRAEEHPTAMAWQAFDLNE
                     VVCELVQALQQADTSGKDVRYRLDLDEANPQIRGNPLLLRQVLINLLDNAADASAFQG
                     EITLTTYGQVLVDGKHYVELTIRDQGPGMADEVLSNLFKPIPTRKAPPHQGLGLSIVK
                     QLMEQLEGVIELRSSGRGTLWSLKFLRVL"
     gene            26640..27563
                     /locus_tag="PKI23_03125"
     CDS             26640..27563
                     /locus_tag="PKI23_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35965.1"
                     /translation="MARLPALIGMMGAHHGAGVTTLSFNLAAALLALDKSVEIIEGEL
                     GSTTATHRLAAARRQSVQQHSGISPIIHQLQEATQLPALLRRLIEPTQRQSAIDFILI
                     DLPSLDITADSAQGEILELLDQLILVTTAQRSSFHATTERLWRLANQEIRLPIGLLIN
                     RVKGYHESQHCFQRLQQEVTPALRSAFDYLGCITCDNGIDLAMTLGKPVALAFPDSPA
                     ALNLRFLVSHKLQHLLSTGRIDRLLWSVDPMQSQPLPAMPVAATAPQGTTAERQPLTA
                     PDEIEAEPSRPNSHGEQSALHRALRLAAALV"
     gene            complement(27594..28409)
                     /gene="queF"
                     /locus_tag="PKI23_03130"
     CDS             complement(27594..28409)
                     /gene="queF"
                     /locus_tag="PKI23_03130"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716074.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNN35966.1"
                     /translation="MSEHGSLLGRQVAHPQRYAPELLEAIPRQAGRLPYGLNEGAWPF
                     FGADRWRAYEISWLMPSGKPMVAVGELTFAAGLPNLVESKSLKLYLNSLNQECFAAMA
                     EVEQRIAADLTARLGGEVRVRLQSLASYHPDWQPLPGSSLDELEVAISDYLPRPELLR
                     VAGDEVVEQLHSHLLRSNCPVTGQPDWGSVLIDYQGVSIDRSGLLAYLISYRCHSGFH
                     EACTEQIFIDILNHCRPRRLTVATWYLRRGGLEINCWRSTQQSAGLNLRLARQ"
     gene            28398..31580
                     /locus_tag="PKI23_03135"
     CDS             28398..31580
                     /locus_tag="PKI23_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN35967.1"
                     /translation="MFAHSDHSSADSTTDCSTISEAFAHHISQTPLIFPQPAIIHPSP
                     TLSHPVPRGLRGGFGLILLILLLLGCQEPQPADPATQIDTARQKWQEGEVDAARNLLQ
                     QLLTRYPRLSPARTLLGRIELAQGNLDRAEHHLRHDLGCVDDGDQARLAYAELLRARG
                     DLQQLSLLLAADLLSPAADNSARHHFLLAEVASGRGDWASALDHYRRAIQIDPRMSEA
                     QLGLLRTLIRQGQDAEALQQITLLLNEPAAPAELFELRAEIEARQGRLETAVQSLEMA
                     IALAPNRAEARIAHARLRLRMRQPDLARQEIAVLLKQMPDSIAVRLLDADLHEQLGDR
                     DGTISRLREIFLLDQNQSDALGRLGTLLYQRQRWREAEIYLDRWLTLSQRGSTDEVAI
                     RQMLARSQLQLRNLTAAEKNLLWLRTHGGGDASEVDLVKLYLLGGRTSEGVSLATALT
                     ERLPQNDAPLANQLAELLLQQNQTQPALKLLSRITSTQPQTPPPTRLLLIRTLIASQD
                     LDRARRELESWHQRDTASALAWQAAGEVAAARGQWPEAQSAWQRARAIDDSDPRLWLD
                     IATAARMQNRADEGRRLLDEALVRFPDQLELQLTAADFERMTHHPENYHSRMKQLIER
                     HPEEAVPRIRYARTLLELGQAEAAQLWLVPLETHEERNADYHLIAAQVALKLADANSA
                     VIHCKRLTQLQPNSAAHQQLLGDAYLLQNEPLSAASAYERAWQIEPANGLAFTAMIQT
                     QLRLQKHAMAQRAILRASKQPTGLDAQTLASAAATVAEAQLEAGQSAEAEQLLEQLPK
                     LTLTTPRLLALRIELLIASNAAAEAERLLSLLRQIEQEGPYSHFAQARLLQQRGRQAE
                     AIAELQQSLARVPLPRAHLALLDLLLERNAYTEAEAQATAWQHQHPDDASNLQRLARL
                     SQHNGNSEQAIAQYQALLKLQPGQIDAMANLIRLLEPSDPALALGYAKQAYLLKPGSA
                     EISGLYGWLLLRTRQSPDQAQRLLLSAHQSQTDDSEILYHLAQAELALERKAEAIKHL
                     RVLAERENDPYREQAGALLDSLAWRPSQPN"
     gene            31668..32750
                     /locus_tag="PKI23_03140"
     CDS             31668..32750
                     /locus_tag="PKI23_03140"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNN35968.1"
                     /translation="MPHSLENINVISQDVLLTPVQLKREIPASELAQSTVAAGRQTLC
                     NILDGKDHRLFVVVGPCSIHDLDAAREYAGKLRQLARQVEDTLVLVMRVYFEKPRTTV
                     GWKGLINDPYMDDSFRIDEGLRMGRRLLVELGELGLPTATEALDPITPQYLQDLIAWS
                     AIGARTTESQTHREMSSGLSMPIGFKNGTDGGLEVAINALKSVKHPHSFLGINPEGQV
                     SVIRTRGNRYGHIVLRGGNDRPNYDSVCIAICEQQLAKARIPANIMVDCSHANSNKDP
                     ALQPLVLEDITHQIQEGNQSIIGVMLESNLEWGNQPIPADLTQLKRGVSVTDACVDWK
                     TTEEALLLMRRRLVDHLPQRRRSLGS"
     gene            complement(32810..>33269)
                     /locus_tag="PKI23_03145"
     CDS             complement(32810..>33269)
                     /locus_tag="PKI23_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNN35969.1"
                     /translation="MGAPAWAEDDRFQTLSGRLTHQALLDQHLGDWTRDQDGRALMYL
                     LQPEVAAAPVQSQSDLYEDPQVLYRGYFVELEHSVMGRVPYNGSQAVLSQTPALLSRA
                     SPCVGEDSLYVLEQLLGMDPAEVDQLVAAGVVEVSASGRKGGRTAHTAAG"
BASE COUNT         6376 a        11152 c         9938 g         5803 t
ORIGIN      
        1 gtgctgctgc gctggcgcga cttcaccttc gcgctcatcg agcatcttga cgtagccgcc
       61 gagcgggatg gcggccagta cgaactcggt gcctgcccgg ctcgtccagg catagagggg
      121 tgcgccaaag ccgatcgaga agcgcagcac cctgacgccc atgcgccgag caacccagaa
      181 atggccaaat tcgtgcaccg tgacgatcac ggtcaacacg atcacaaagc ccagcagggt
      241 ttcaaacatc gtcatgaatg ctttgcacgt tgctaaaaaa gagaagcatc tgtcgtcgtc
      301 gcgtcatttc aggcgccgcc tcagccattc gttggccagc tggcgtgccc gcatgtccac
      361 ttccagaacc tgctcgatgg agtcgactcg aagtggaacc aactgctcca gtacctgctc
      421 aagcaatgcc accatctcgg taaacagcag tctgcgctgc aaaaaagcgt cgacggcgac
      481 ctcgttggcg gcattcagca cggcacccgc gccaccgccg gcgcgcgccg cggcaatcgc
      541 caatgccagc gcaggatagc gggccagatc gggtttggag aactccaact gaccgatggc
      601 caccagatcg agtgctggaa caccggacac gatgcgctca ggccaggcca gtgcatgggc
      661 gatgggtgta cgcatgtcgg gattgccgag ctgcgccagc gtcgaaccgt cgacgtactg
      721 caccatcgag tgaatgatgc tctgcggatg aatgacgatg tcgacctgat ccggcgtggc
      781 atcgaacagc caacaggctt cgatcagttc cagccccttg ttcatcatgc tcgccgagtc
      841 gaccgaaatc ttgcgcccca tctcccagtt gggatgggca caggcctgct ccggggtcac
      901 cgattggagt tgatcgatcg gcagcgtccg aaatggcccg cccgaagcgg tcaggatgat
      961 ccgatcgatc accctttcat caaatatttt cttggaacat cccgcaatac attgaaatat
     1021 cgcgttatgc tcactgtcta tcggcaacag ttgtgcgccg gaatctgcca ccgcctccat
     1081 gaagagctcg ccgctcatca ccagactctc tttgttcgac agcaacactc ttttgccaga
     1141 acgcacggca gccagggtcg gcagcaatcc ggccgctccg acgatggcgg ccatgacggt
     1201 atcaacccga ctctctgcgg ccacttggca gagtccctct gccccccaca gcacttcggt
     1261 ggcgagctcc gccgcacgca gccgcgaaat cagagcgtcg gccagtcttt catccgccac
     1321 cacggcatag agcggattga attgccgaca ttgcgcaaag agcagatcga cgcgtcggtg
     1381 cgccgtcagc gcaaacacct gatagcgctg cggatgcagg gcgatgacat cgagcgtgcg
     1441 ggtgccgatc gagccggtcg agcccagaat ggtgatgcgt tgcatcagcc atccacctgc
     1501 tggctcgaac agagcatgac ccaggcaaac cagggtgcgg ctgcgcagat gctgtcgagg
     1561 cgatcgagca atccgccatg gccgggcagc agatggctgc tgtccttcac gccccggtaa
     1621 cgtttcagca tgctgatcga cagatcaccg accactgaaa acagcatcgt cactgccgcg
     1681 atgcccatgg ccagcaggct ccgctgcagc gacagaccct gcgcgagtgc aatgccagca
     1741 gccagcgcca gggtggcgcc cagccccccg gcaaagccct cccaggtctt gccggggctg
     1801 acgcgcggcg ccagcttggt ctgcccccag gttcgcccga ttgcgaatgc gccgatgtcc
     1861 gccgccgcga ccagcgcgaa gaaatagagc agatgccact cgccattggg cagtttcttc
     1921 aatgcggcca aggccagcca ggtcgccatc aacgaccacc aaccccagag agttcgcatc
     1981 agcggtgcct gccacagcgc tgacgcttcg ggatagcgca ccagccatgg aattggcaac
     2041 agccagacga tggcagaaag cgcaagcggc agacgcagca gatcgagatc ggtatcgatg
     2101 agccagtagc tgccgcccac cagcagcagc atgacgccga cgcagagtcc gcgtacggca
     2161 agccgttcga tcgatgcaag gttgaaaaac tcccagcttg ccgccagggt gatgagcgca
     2221 gtgatgaggg cgaactgctt gaacgaggtg gccagaatca atccgagcag cagcgccccg
     2281 agcaggatgc cggtgaggat gcgctgcctc aacatccggt ctgctcttga ctgggactct
     2341 cctgatcacg gcgcccgaat cgccgctcac gctccatgta gctgtcgatc gcccgcgcca
     2401 gctcctcttt ctggaaatcg ggccagaagg cttcgctgaa gtagaattcg ctgtaggccg
     2461 cctgccacag caggaagttg ctgatgcggt attcgcccgc tgtgcggatg tacagatcgg
     2521 gcggtggcag atcggccagg cagacataac gctgcacggt ctcttcatcg atccgtgccg
     2581 gatcgatcag cccagccatg gcatccatgg cgatgcggcg cgcggcctgg accatgtccc
     2641 agcgcccgcc gtagtccgcg gcgatcacca gcgtgctctt tgcgttgtct gcggtcagcc
     2701 gctccgcgcg ttcgatgtgg cgccgaatgc tcggatgaaa acggtccctg tcaccgatca
     2761 cacgcaaccg gacgccattt tggtgcaatc gactgacttc gtacttgagt acggccagaa
     2821 acagcgccat cagcgcacgc acttcaccag ccgggcgttg ccagttctcg gtgctgaacg
     2881 cgaagagtgt cagcacctcg atctgctgct cgacacagta ctcgaccacg gaacgcaccg
     2941 attcgattcc ggcacggtgg ccggccttgg gtgacagacc acggcgtttg gcccaccggc
     3001 tgttgccatc catgatgatg gcgatgtgcc ggggccgaga ggatgggagg agatcctcac
     3061 ccgaaggcaa aactgtttgc gccatcatct tcgaggctgc ttcggtcata ccgacctgca
     3121 ctatgcttat tagaaggtgc cactgtaggg atcaatcgac accgaaccac tcagaaagac
     3181 gaaaaactaa atctccatca gatcggtctc cttggtggcc aacagtgcgt cgacctcctt
     3241 gatgaaccga tcggtcaatt tctgaatctc ttcctcggca cggcgcaggt catcttccga
     3301 gatctccttc tctttcagca gctccttgaa atcgccgttg gcatcgcgcc ggatgttgcg
     3361 gatggccacg cgcgcctgct cggcttcgtt gcgcgcctga cggatgtggt tcttgcgcgt
     3421 ctcttcggtc aatgccggca tcggaatgcg gatcagctca cccgaagagg tggggttcag
     3481 acccagaccg gcactcagaa tggctttctc gatggccggc agaattttct tctcccacgg
     3541 cgagacgatc aggtttctcg aatcgtccac gctgacgctg gcaacctgct tgagtggcgt
     3601 gtcggtgccg tagtagttga ccttgacgcc atcgagcagg cctggactgg gccggccggt
     3661 gcggatttta ctgaaggcga cacccaatgc atccaccgcc ttggacatgc gttcgctggc
     3721 gtcctgtctg atctcgttga tcacggtctc aacacccctt ttcggataga tctattcaat
     3781 ctgcgttcct tccgagccgc caacgacaat gttgagcagc gcccccgctc gatccatcct
     3841 gaatacccgc agcggcatgc catggtcctg acacaggcag atcgcggtca gatccatcac
     3901 tcccaatttg cgatccagca cctcctggta ggtgagccgg tcatacttga tcgcggatga
     3961 gtcggtgaag gggtcggcag agtagacccc atccaccttg gtcgccttga gcaccagttc
     4021 ggcattgatc tcgatgccgc gcagacaggc cgccgagtcg gtggtgaaaa aggggttgcc
     4081 ggtgcccgcg gcaaagatca gcacatcgcc ctgctccaga tagcgaatcg cctcgcgccg
     4141 gtcatagtgt tcgaccacac cactcatcgg gatggcggac atgacgcgcg aagagatgtc
     4201 cgagcgttcg agcgcatcgc gcagcgccag gctgttcatc accgtcgcca gcatgcccat
     4261 gtggtcaccg gtcacccggt cgagccccac ctcatgcagt tgcgcgccac gaaacaggtt
     4321 gccgccgccc acgaccaaac cgacctgcac gccgatgccg accagctggc caatctcgag
     4381 cgccatccgg tcgagcacct tcgggtcgat gccgaagctc tgatcgccgg tcagcgcctc
     4441 tccactgagc ttgagcagga tgcgcttgta cttgcggtct gttgctgcca ttgcgcgggc
     4501 gacctccggg ttcagctcaa gcctgcctga gcagccactt cggccgcgaa gtcgacctgc
     4561 tccttgacga tcccttcacc cacttcgtaa cgggtgaacg agagaatctc ggcgccggcc
     4621 ttcttgacca gatcgcccac cttgacatcg ggatccttga cgaaaggctg ttcgaccagg
     4681 ctcacctccg ccaggaactt gcgaatgcgg ccatcgatca tcttctcgac gatttcggcc
     4741 ggcttgccgc tctcggccgc ctgcgcgctg tagatctcgc gctctttggc gatcacgtca
     4801 gcggacacat cggtgcttcg gaccaccagc ggattgctcg cggccacatg catggcgatg
     4861 tcgcgggcca gcgcgctgct gccattcttc agcacgacca gcacgccgat cttgttgttg
     4921 ccatgcacat agctgccaac gatggcggaa tcggcctgct tgagttcggc ccggcgcaca
     4981 ctgatgttct cgccgatctt ctgcaccagc gcctcacgca cggcacccag ctcggagccc
     5041 atcagtgcat cgacatcgac tgtctttgcg gaaaacaacc gatcaaccac ctgccgcgag
     5101 aagttgagga aattttcgtc ccgggccacg aagtcggttt cactgttgat ctcggccatg
     5161 acgccgtagc tgccatcttc cgccagtcgg gtgacgatga ccccttccgc cgcgatccgt
     5221 cccgcctttt tggcggcctt catgccggaa gatttgcgca gctcatcgat cgccttctcg
     5281 atgtcgccat tggcctcctg cagcgcgcgt ttgcactcca tcatgccgag gccggttcgc
     5341 tctctcagct ccttgacctg ggaagcactg attgcggtca tttacactct ccttccgaat
     5401 ttcatccgct gcgcgacagc tgcagcggcc gttcatcttc tgcccgagca gcgggtgagc
     5461 gcgcagcgca cccatccgcc cgggccttca tttctgcaaa agcggcagga ttccggccga
     5521 ctcagaccga cgccgactca tcctggactt cgacgaactc atcctgacgc accacaccgc
     5581 gcgcccgctc cttgccgtcg atgatcgcat cggccactgc ggtcacatag agctggatgg
     5641 cgcgaatggc atcgtcgttg cccgggatca catagtcgat gccttcaggg ttgctgttgg
     5701 tgtcgacaat ggcaatgacc ggtatgccga gcttgcgcgc ctcggtgatg gcaatgtgct
     5761 cgtggtcgac atcgacgacg aacatcgcgt cgggcaggcc gttcatctct ttgatgccgc
     5821 cgatgctgcg ctcgagtttt tccagcgtgc gcatccgcat cagcgcttct ttcttggtga
     5881 tcttgtcgaa ggtgccatcc tgactctgtt tctcgatctc ctgatagcga cggatcgagc
     5941 tgcgaatggt cttgtagttg gtgagggtgc cgcccagcca gcggtggcag acatagggag
     6001 cgccgcagcg ttcggcctgc tccttgatga tcttgctggc cgagcgcttg gtgccgacaa
     6061 acagaatctt gctgttgctg gcggcaagat caccaagcag gccgagggcg cggttgaacg
     6121 ccggcagggt gtattcgagg ttgatgatgt gaatcttgtt gcgggctcca tagatgtagg
     6181 ggcccatttt gggattccag taccgggtct ggtgtccgaa gtgcactccg gccgccagca
     6241 tctctttcat gctcacgtgt ggcataacgc ctcctgttgg gttgaacctc cacgcatccc
     6301 atgattcaac cctgtgagag gggcacccag aaccatgtga cgatgcgtgt gtgtcgtttc
     6361 gttcgttgaa ttgaggtgtg cttcgcaccc cccctgcccc tcgtaaaaag aggcagggcg
     6421 cggattatgc cacagggcgg gatgcatcaa aagccgaagc gggtttgatc gaagctggcg
     6481 gcactccagg tggatacggt tgtgatcgct ccgcgaaacg ggcggaccac cgccccgtca
     6541 ccgttcagct gttgggttcg ccgaggacga tcaccttgtc ggccgagagc ccctgcgcgg
     6601 cgcaaagcat ccggtaggtt ctgagaatcg cggcagcatc gaccatgccg accacattgc
     6661 cggcctcgtc gatgttgatg ttggcgccat agatcgcgaa ccagacatcg gtaatgcctt
     6721 cctgatcctt cggtgtcatc agcgtgtgga ccgaaccggc gggttcatag aggtaggagc
     6781 cgggtgcgtt gacgtagctg ggatactcct tgtagaacca gcgccccttc agggtgaccg
     6841 caaagaccga tccggtgtgg tagtgcttgg tgatggtgcg tccgggcggc atgcgggtgc
     6901 gcagaatcca caatccctgg ttcagatcga cctgcaacaa ctgcatcgaa ccaccatcgc
     6961 cggtgtcgac gaagggcact tcgctctcgc tgatgtggat ggcttcggtc aggccatgca
     7021 gcggaatcat gggtggcgga ttggcaggcg cagtggacat gcgggcttcc tcaaaggggg
     7081 agtggacagg gatgtgtctg aaaggtggcc ggcattgttg tggtggcgaa ccccggaccg
     7141 actcacccgg ccgcatcata gcgcatccct gtggtcacgc catcagactg atcgccttgc
     7201 gaaaccgcgc cagcgacagg ccgaacagtg ccgctccgat caggccaagc gcggcaaagt
     7261 gaggccagac caccccaatg ccggcaccgc ggtagagaat cgcctgcgag aaggcgacga
     7321 agtgggtggt gggcgccagc gccatgatcc actgcaccag ctccggcatg ctctcgcgtg
     7381 gcgtcactcc gcccgagagc atctgcaacg gcagcagcac cagcatcagc agcaggccga
     7441 actgcggcat cgatcgggcc agggtgccga ggaagatccc catcgaggtg gtggcaaaca
     7501 gatgcagcgt ggcgccggcc agaaacagca gcagcgaacc ttcgatcggc accgccagca
     7561 atccctgcac cacgccgagc agtgacaggg tgcaggcgac cagcaccacc agccccatcg
     7621 cccacacctt gctggtcatg atctcgaacg gcgtcaccgg catcaccagc agatgttcga
     7681 tggtgccatg ctcacgctcg cgaatcagtg ccgcgccggt cagtacgatc gacagcatgg
     7741 tgacgttgtt gatcacctcc atcaccgaac cgaaccacgc cttgttcagc tccggattga
     7801 agcgcgcccg cagcgtcagt tcgaccggca acacactgtt gctgcggtga cgctgaacga
     7861 attcggccac ctcactgttc accatgctct ggatgtaacc gctaccggca aaggcctgac
     7921 tcatgcgggt cgcatcgacg ttgagctgga tggtcggtcg acgcccggcc agcaggtcac
     7981 gctcgaaatc gggcggaatg tcgagcgcga aggtgtcgag cccggcattc atccgcgcat
     8041 ccatctgcgt ctgattgatc agcaccggcg gcatgaaatg gggtgggtag aatgcgccga
     8101 cgatgcgcgt cgacagcgtc gaacggtctt cgtcgacgat cgagatggcc gcccggttga
     8161 ggctctccgg cattgcggtg gcggcggaat agatggcgaa cgtgaacgcg aacacaatca
     8221 gcaccaacat ggtcggatct cgagaaagac ttctcaattc cttgattcca agatttaaaa
     8281 tgttggaaat gcgcatctca tcgctcctgt tttttcagca gcagcacgca gaaggtgaag
     8341 atcaccggag tcgccagcag cagcgccatc atctcggttt gcaggtcggc gaaaccgagt
     8401 gccttggaga agaccccccg tgcgacgatc aaaaaatggg tggtcgggta gatctggccg
     8461 atcagccgac ccactccttc gagcgaggag accggatcga tcatgccgga gaactgtatc
     8521 gccggcagca ggctgagcag cgcagtgccg aagatggcgg cgatctggct gcgcatgaag
     8581 ctggagatca aaagaccgat ggccgtcgcg gtgcagacat agagcagcgc ggcggcggcc
     8641 agcgtcagaa agctcccctt cagcggcacc ccgaacagcg tgacggccag cagggtcagc
     8701 atcaggaagt tcagcatcgc cagcgcgacg tagggaagct gcttgcccag cagaaactcc
     8761 atgcgggtca ccggcgtgac gtagaggttg atgatcgaac ccagctcctt ctcgcgcacg
     8821 acgctgagcg cggtcagcat cgccggaatc atcatcagca gcagcgggat caccgccggc
     8881 accatcgcca ccagactctt gacgtcgggg ttgtagcgaa agcgcagctc gatgttgacc
     8941 aatccctggc tgccggattg accggaacgg cgcagttgca ggtcggccag ccacttcagg
     9001 tgcatgccgc gcacatagtt ctgcacggtc tcggcgcgct gcggcatggc gccgtcgatc
     9061 caggcgccaa tctccaccgg gcggccacgt tgcaggtcgg cagcaaaacc cgggggaatc
     9121 tccagcgcca gactgatctc gccactgcgc atgcgtctgt ccagttcggc atggtcggca
     9181 atgggatgac gctcgatgaa gtaacgcgag ccagagaggt tcgagacata gtcacggctc
     9241 agcgtgctct gatcgcggtc gagcacggca aaggtgagat cctcgacatc catgctgatg
     9301 ccatagccca tcaccagcat cagcagcagg ctgccgagca gcgccagcgt cagccggatc
     9361 ggatcacgtc gcaactccaa ggcctcgcgc cggctgtagc tgaacatgcg ctccggatcg
     9421 aaacggcggc gccggattgg cgtggtcgcc gatgccgagc cactcgccac atcattggcc
     9481 acctcgacca cggcatgcgg gggctcctca tcgaccacct cgcgcagatg gctgatgaag
     9541 gcctcttcga gactcccgac tccacgctcc gccaccagcc ggaccgggct gtcgctcgcc
     9601 agcaccttgc cggcatgcat cagcgagatg cgatcacagc gctcggcctc gttcatgaag
     9661 tgggtcgaga cgaagatcgt cacctggtca ttgcgtgcca ggtcgaccat gatctgccag
     9721 aaggcatcgc gggcgattgg atcgaccccg gaggtgggct cgtcgagaat cagcatctcg
     9781 ggctgatgga tcatcgccac cgccagcgac agccgctgac gctggccaag cggcagatcc
     9841 atcggcagca tctccatgat ctccagcagc ccaaagcgct ggcccatctc cagcacccgc
     9901 cgcgcgacct gtgccggtgc cacgccgaac aggcgcgcgt gcagcagcag gttctgccgc
     9961 accgtcagtt cggcgtagag cgagaagccc tgcgacatgt agccgacccg gcgccgggtg
    10021 tcgatcgcct tcggatcgac cggctgaccg aacagccagg catgcccctc gctggcgggc
    10081 agcaggccgg tgagcatctt catcgtcgtg gtcttgccac agccattgga gccgagaaag
    10141 ccgaagatct cgccgcgcgc gatgcggaag ctgacattgt cgacggccac gaagtcgccg
    10201 aagcgcatcg tcaggccact ggcctcgatc gccgcgtcgc catcgccatc gggctggcgc
    10261 ggcggaatca ccaccggccg atgttcacgg cggcgcagct ccggcagcag tgcgatgaaa
    10321 gcctgctcca gggtctcgct gccactttgc gccagcagtt gcgccggcgt gccactggcc
    10381 agcacggtgc cgccatccat cgccaccagc cagtcgaacc gggccgcctc ctccatgtag
    10441 gcggtggcga ccagcacact catcccggcg cggtggctgc gaatgcggtc gatcagctcc
    10501 cagaactgca cgcgggagag cggatcgacc ccggtggtcg gctcatcgag aatcagcaga
    10561 tccgggtcat ggatcagcgc gcagcacaga cccagcttct gcttcatgcc gccggagagc
    10621 ttgccggccg gacggtcgcg gaacggggca aggccggtgc tgatgagcag ttcgtcgatg
    10681 cgccgccggc gctcaccatc gccatggcca aacagccggc cgaagaaatc gatgttctcg
    10741 aacaccgaca gcgtcgcata gaggttgcgg ccaagcccct gcggcatgta ggcgatgcgc
    10801 gggcagaccg ccagtcgatg gcgccgctgc gccatgtcgc catccagcac ctcgatctga
    10861 ccggtctgga tctcgcgcgc accggcaatc agcgccagca ggctcgactt gccgacgcca
    10921 tccggtccga tcagccccac ccgctgaccg cgcggaagct ccagtgtcac cgcctcgagt
    10981 gcccgcaccg cgccgtggtg cagtgcgacg tcgcgcaggc ggacaaccgg tgtcaatgtc
    11041 ggatcggcag cactgtcact cattgcggca gtttgacctg cagctcttcc ggccactgcg
    11101 cctgcggatc gatccgcaca taggccatgc ccggcaggcc ggtcttgatc gagcggagat
    11161 gctgtttgag cagctctggt gcaatccgcg ccttgacccg gaacatcagc ttctcgcgct
    11221 cactggcggt ctcgacggtc ttgggggtga attgcgccac atcggcgacg aaggagatct
    11281 gcgccggaat cacatactcc ggcgcggcat cgagccgcag acgcacctcg ctgccgatcg
    11341 ccagccggcc ggcggcggca gtcggcagaa agaaggtcat gtagacatcg gtcagatcga
    11401 gcacattgag cagtcgactg ccggcaccga ccacctcgcc cggctgggca atgcggtact
    11461 gcacccgccc atcgcgcggc gccttcaagg cgctgtcatc gatgtcggcc tgcagccgct
    11521 cgatggtggc gcgaccggct tcgacggcgg ccagcgcgcc ggtcacctgc gcctgggccg
    11581 tcacgattgc cgcctccatc gccaccacct gtgctttcgc gccattcagc gctgcttctg
    11641 cactcgctgc gcgggcacgg ccatcgtcca gatcctgcgc agtcaccacg cccttggcgg
    11701 ccagcagttc ggcccgcttg agccgtttgc gggccacatc cagctcagcc atgcgctggg
    11761 ccacaaccgc ctcggcggcc tttttttcac tctcgcgctg tgccgcctga ctgcgggcgg
    11821 tggccaccgc gctctcggcc tggcgcagcc gcgcttcggc ttcacgccgc tgcgcagtga
    11881 gcacctcggt atccatctgc gccacctgct ggcccgcggt gacgaaatct ccttcatcga
    11941 ccagaatcgc ctgcactcgt cccggcacct tggccgccac atcgacctcg accgcctcga
    12001 tgcggccatt gccgctggca aagcccgcac ccaacggcgt tggttgcagc cgctgccaaa
    12061 ccagatagcc aattgccacc agcgcgatca gcgcacctgc cttcagccat cctccaagcc
    12121 tcgatttcat accccatcca ttgcgtcaaa gtgaaaaaat aatgagttta cagcgtcatg
    12181 aagcctgcag agcctgactt cgcgcaacca tctccatcaa aacagctgat ccaagagatg
    12241 gcaagacgcc ggacttggtc atgggtcaga cagactgcta aagtgacgaa aaaaggagct
    12301 gaccatggac aagctgcact atcagataca ggactcggtc tgcacgattc atctggatga
    12361 cggcaaggtc aatgcgatga atctggtctt tttcgaggag ctgcatgccg cgctcgacca
    12421 ggcggaaaaa ttccgctgcc gggccgtggt gatcgatggg cgggagggcg tcttctcggc
    12481 cggactcgac ctcaaataca tgccgacgct cgaaccggcc gagcgtgccc gcttcagtca
    12541 gacctttgcc cgcgccattc tgcggctcta cacgctgccg atcccttcgg tggcggcgat
    12601 tgccggccat gccatcgccg gcggcgcgat tctggccttt gcctgcgatt gccgctacgc
    12661 gatcgatggg cactatctga tccagctcaa cgagatcaag aacaggatgg cgctgccgaa
    12721 ctggatgagc ctgatcgcct cgacggtggc cgcacccgcc accctgaccc agttggcgct
    12781 gcatgcccgc gcctaccgcc cgcgcgaagc ctttgcagcc cagcttctga ccggattggc
    12841 cggcgacggc aatgagctga acgaactgct ccagcagtgc tgccgcgact tcttcgagtt
    12901 cgaccccgcc agttacgcca ccaccaaacg ctggatgcgc aatggtgctg ccgagcaggc
    12961 gctgtcgcat ctgaaggatg tgcaggccac actgatgtga tgtgcggctc gggccgacca
    13021 ccgtccgggc catttcacca ctcagaaggg ctgggtctcg ccgatgtgcg gaatccagat
    13081 ccgttcggcc gccaccccct gctgcgtggc ggcagcgcga aagcgtgtcg gcgcctccat
    13141 caatccatcg aagccgagct gtacggtgcc ccagtgcatc gccgccatcc gccgtgcccg
    13201 cagatcgagc cccagccgca ggcagtcggc cggttcgcag tggtgtcccg ccatcacctc
    13261 ccgcggcaga aaggccccaa tcgacagcag cgccaggtcg aacggcccat actgctcgcc
    13321 caactggcgg tagtggggac catactccgc atcgccaccg aagtagagcc gcttgccggt
    13381 ggccgagacg atcgagaagc ctgcccacag ggtgtcgttg gtcttgaaca gcgtgcgctt
    13441 cgaccagtgg atcaccggca gcgcagtgat gcgcagtccg gaaaactcac tgctctgatg
    13501 ccagtcgagc tccaccacct gcgtcagtcc ctgctcacgc accagctcac cgagccccag
    13561 cggcacgatc accagcggat tctgccgcct ggcgagctgc cgcagggtcg cagtgtcaaa
    13621 atggtcgtaa tggctgtggc tgatcagcag caggtcgatg cgcggcagac tctccaccgg
    13681 cagtgctgga gggaccaccc gcctgggacc aaagggcggc agcggcgaac agtggtcgct
    13741 gaaccaggga tcggtcagca gggtcacgcc gcccatgcgc accagtgcgg tcatgtgacc
    13801 gatccaggtg acactgtcgc cctgggcgtg gcgatcgagc cccgcctgca cctgcggggc
    13861 cgaccagcga tggtctgccg gcaattggct catgtcggcg cgctgaaacg gaaactgcac
    13921 gatgaagggc agcttgcgcc accaggagcc ggtgggcgga ctttgcggga gattgcgaaa
    13981 gccacccccc ggaagatggt gcgatggtga ctcggcagag tgtccggcac tgtcgcagcc
    14041 agccagaaaa gcgcccagca agagtgcgag cagcagcctg ttcagtggca gacgaagtcg
    14101 gtttgtcatt ttcgattcag cgcgtgtatg gtagaagtcg atcggggcaa ttgctcatga
    14161 ggcatggcat gcagttgaag gatcgcgcac aggggtacgg ctggatcagc tggctgctgc
    14221 actggccgtc ggcgctcctc attgtcggcc tctttgcgct tggactctac atgcgcggcc
    14281 tcgactacta ccacccctgg taccaccggg ccccggcgct gcacaaacag atcggcatcc
    14341 tgctcgccac catcaccctg ctgcggctgt gctggcggct cttcaaccgc acgccgccgt
    14401 tcgaagcgat gctcgcacca tgggaacgaa tcgtcgcaac tctggcacac cagctgctct
    14461 atctgctgct gttcctcact ctcgccactg gctatctgat cgccaccggc ggcggtcagg
    14521 cagtgtcgct gaccgacatg ctgagcatcc cctcactggt tcgactgcac gacagcgaca
    14581 ccgaagaact cgtgggggaa ctgcatgagc tgttcagttt tctgctgatc ggcctgaccc
    14641 tgcttcatgc cctggccgcc tgcaagcatc actggatcga ccgaaaccgc accctgctgc
    14701 gcatgctctg gccgggttcg acatccccca ccgcccctca ggagagagaa ccatgaaacg
    14761 ccccttccac cgccgattgc ccctcgccgc caccgtggca ggcctgttgc tgagccccat
    14821 cggccaggcg gccgactacc tgatcgatac caagggcagc cacgccttca tccagttccg
    14881 catccagcat ctggggtaca gttggctcta cggtcgcttc aacagtttcg atggccggtt
    14941 cagttgggac gaaaagcagc cgaccgcctc gaaggtggag gtcaacatcg atcccgccag
    15001 catcgactcg aaccacgttg aacgcgacaa ccatctgcgc tcaaaggatt ttctcgacgt
    15061 caaacagttc ccgaccgccc acttcgtcag cacctcggtc gctgcggagg ccgggggcgg
    15121 gctgctgctg aagggcaagc tcagtctgca tggcgtcacc cgcgacatcg acatcaagat
    15181 gcacaagcta ggcgaagggg ccgacccctg gggtggctat cgtgccggtt tcgctggcac
    15241 cacgacgctc aggctgaagg actatggcat ccagcgcgac ctgggccccg cttcgcagga
    15301 ggtggagctg acgctgtcga tcgagggtgt gcgacaatag ccgccctgtg caaccggggc
    15361 ggcttgattc agcatcgatc gcccccattg cacaacgaca ccccgccggg gtcaggcagc
    15421 gcctcttcat catcccgcga gaccatccat ggctgaacgc caactctata aagtgatctt
    15481 caccaaccag ggcaaggtgt acgaaatcta cgcccggcag atttaccaga gcgagctcta
    15541 cggcttcatc gagatcgagg agctggtgtt cggcagcacc acccagctgg tggtcgaccc
    15601 gagcgaggag cgactgagga gcgagttcga ttcggtgacc cgcagctaca tcccgatcca
    15661 ctcggtggtt cgcatcgacg aagtggagcg cgaaggcgtc gccaagatca gcgaatcaac
    15721 cggcggcagc aatgtcaccc cgtttcccct tccggccttt cgccccggcg gtcacaagga
    15781 gtgaccgctg caaggagacc tttcacgctc ggctggccag tcgctgcctg agtgtcgcca
    15841 gttcgacaca gagaacgaag acctccatgc tccgatcgat ctcctccagc gagggaaagg
    15901 agggggcaag acggatgttg ctgtcctgcg gatcgcgccc gtaagggaag ctggctccgg
    15961 ccggcgtcag cgccactccc gccgcgcgac agagcccgac cacttcgctg gccaaccccg
    16021 gcagggtgtt gaaggagatg aagtagccgc cccttggact ggtccagctg gcgatctcct
    16081 tgccccccag tgcagcctcc agccgctgca gcacccggtc gaacttcggc cgcagaatct
    16141 cggcatgctg ccgcatcaag gcgcgcaggg tcgactcatc cttcagaaag cggacgtgac
    16201 gcaactggtt gaccttgtcc ggaccaatgg tcgacatgcc gagatgggca cgaaaccggg
    16261 ccagattggc cggtgccgag gccagaaaag agatgccggc cccggcatgg gtgatcttgg
    16321 aggtcgaacc ggtgatgaag atcgacgtct gggtgcccag cctccgtgcc agctccatca
    16381 gattggcgag cgcatcgccc ggcgtatgga ggtcatgcac cgtgtaggcg ttgtcccaca
    16441 tcaccacgaa atccttcggt gcgatgcgac cgagcgccgc gatgcgggcc acggtctcgt
    16501 cgctgtaggt ctcgccggtg ggattggcgt atttgggcac gcaccagatg cccttgatcg
    16561 aaggatcctg cccgatcagc gcctcgacct gcgccatgtc ggggccctga ccggtcagtg
    16621 cgaccggcac catctcgata ccgaggtgct gacagatcga aaagtggcgg tcatagcccg
    16681 gcaccggaca gagaaacttc actccgccct gttcgcgcca gggcgtggca tccggccgca
    16741 ggccgaagaa gtgagagaaa aacagcaccc gatacatcaa ctcaaggctg ctgttgccgc
    16801 caaccagcac ctcgtcactg ccgacctcga gcagcgcagc gcccaaccgc ttggcctcag
    16861 gcagaccatc gagcccacca tagttgcgtg catcggtgcc atcctcggcc agatgtccac
    16921 cctcgagcgg acccagcagc gcgtcggaaa gatcgagctg ggccggactc ggcttgccac
    16981 gggtcagatc gagcttcagc ccggccgcac accactgtgt gtaggcgtgg ctcaacacct
    17041 gttcacgcgc caacagttgc tcttctggaa gctgaagcag tgacaagaga gactccttgg
    17101 gcaggtttgg aaggggtgga ccacccacga ccgcgccggc aacagagcgc cagccgatca
    17161 ggggcggcca ttataaataa gacgaacggt tcttgcccgg cgattgccga atgaagccgc
    17221 cagcagatcg cctcagcggc gcggcctggt cggcgccgac ttgccacctg ctggccggcg
    17281 agccggctgc ttccgtgcag tctgcggtcg gttgctgagc gcatcgtccg gtgtgcgccg
    17341 acgtggtggc cgctccgccg cgcgctgcgc cgcggcagca ggcgatgacg ccgaacctct
    17401 gccgcgcgcc ggagcggttt cgcgggtctc atccgccgtg cgtgaccgcg ggctgcgtcg
    17461 aagcgatgcg ccgaaacgcg gcgccgccgg cgcgtctgtg cgctcccgtc cgaccatgcc
    17521 acggccacga ccggcgggtt ttctggtacg ttcggcggtc gcctcgtcgg ccacgaaatc
    17581 ggccggcagc gggatgttgg cgtagcggca cagcgcctcg atctgcggtc gctccagctc
    17641 ctggtagcgc ccctgcttca ctgccgacgg cagaaagatc ggcccgaaac ggacccgttt
    17701 gaggcggctg acccgaatcc cctgcgactc ccacatccgc cgcaccagcc ggttgcgtcc
    17761 ctccatcacg gtgacgtaaa accagcgatt gatcccctgc ccgcccccat cgccaagatc
    17821 ggtgaagcgg gccggcccat cctcgagctg cacgccggtc aacagccgtt cgcggacgcc
    17881 ctcgggcagc tcgccgaaca cccgcaccag gtactcgcgg tcgatggcag tggaggggtg
    17941 catcagccgg ttggccagct caccctcatt ggtgaacagc atcagaccgc tgctgttgag
    18001 atcgagtcgg cccacggcga tccagcggcc atacggcagt ttgggcaggt tggagaagac
    18061 cgtcggccgc ccctcgggat cactgcgggt gcagatctcg ccctcgggtt tgtggtagat
    18121 cagcagtcgg gtctgctgct tcaccggcgg cctgatggtc accggacgtc cggccagagt
    18181 caccttgtcg cttgctgtca cccggtcgcc cagccgggcc acccgaccat tgagggtgac
    18241 ctgtccctcg gcgatcaact gttcaagcgc gcgacgggag cccagtccgc aacgggccag
    18301 tgctttgtgc aatttttcgg tcggtacagt catgggtgct ctctggtcaa tggcgtggtt
    18361 ggcggatcaa gttcacccaa ctcgagctgc ggatggctga cattcaggtc gacgagctct
    18421 gccagcggcg gcagttcact gaggctcttg aggttgaaat ggtcgaggag ctgccgggtg
    18481 gtcgcataga tcgccggtcg tcccggcact tcgcggtggc cgatgacggc aatccagccc
    18541 tgttcgagca gactcttcat gatcgagctg ctgacggcaa caccacgcac cgcctcgatc
    18601 tcggcacggg tgatcggctg ccggtagagg atcagcgcca gggtttcgag cagcgcgcgg
    18661 gagtagcgcg ggggtttctc ctgcgccagc cgggtgaccc agggggcgaa ccgcgccctg
    18721 acctgcatgc gccaaccgct ggccacctcc ttgagctcat agccgcgccc ctcggctgcc
    18781 acaccgaccc tggtcagcgc ctcgcgcaac tgcgacagcg acggctgctc tggcgggtcg
    18841 aacagctgct gaagctgctc aggcgtcaag gggcgatccg ccgccagcag caccgcttcg
    18901 acgatctcgg caagcgtcgg ctgcgccatg ctcatgcacg cttcgcctgc agatggatcg
    18961 gtgccagcgg cgcattctgc agcagtgtca ccaacccctc cttgaccagc tccatcatcg
    19021 ccagaaaggt gaccaccacg ccaagccgcc cctcttcgac agtaaagaag agggtaaagg
    19081 ggagaaaacc atcatgcgac tggctgagcc tcgccagaat cgcactcatc cgctcccggg
    19141 tcgacagcag ctcgcgtgac acctgatggt gctcgaacag ccgggcacgc tccagcagtc
    19201 cacgcagcgc ttgcagcagt tcatcgagct gcagcgacgg cggcagcggc gcccgtacca
    19261 gcggcggcag cgcacagttg agcaacgcca gatcgcggcc aagccgcggc agttggtcga
    19321 gccgctcgcc cgcctgccgg aactgctcgt agtcgcgcag ccgccgtatc agttcggaac
    19381 ggggatcttc gctctcctca tcggtggagg caggctttgg cagcagcagg cgggacttga
    19441 tctcggccag ggtcgcggcc atcaccagat actccgccgc cagttcgaag cggctgtcct
    19501 gcatcaactc gacgtaggcc atgtattggc gggtgatggc cgccaccggg atgtcgagga
    19561 tgtcgaggtt ctgccgcttg atcagataga gcagcaggtc gagcggcccc tcgaaggtct
    19621 caagaaagat ctccagcgca tcgggcggga tgtagagccc ctcagggagg cggaacagcg
    19681 cctcgccctg cactctggcc agcggcagct cctgctgcag tggcacaggc aagggcatcg
    19741 gttcagccga cacggccgtg ccgttgcatc agcggtacct cagccccatc acctcgcgca
    19801 cctcatcgag ggtggcacgc gcctcttcac gcgcccgtgc attgccctcc ttgaggatgt
    19861 tgcgcaccag cggcagatcc tgctcatatt cgctcgcctt cagttgcagc ggccgcagct
    19921 caccctggat cgaatcgatg atcggccgct tgcagtcgac gcaaccgatg ccggcactgc
    19981 tgcaaccctc gcgcacccac gcctgctgca cgccatcgct gtagatctga tggaactgcc
    20041 agaccggaca cttgtccgga tcgcccggat cgctgcgccg cacccgcgcc ggatcggtct
    20101 gcatcgtgag aatcttcttt tcgatcagct cgggcgggtc gcgcagcgcc agcgtgttgc
    20161 cgtaggattt cgacatcttg cggccatcga gcccgggcat cttcggaatc ggcgtcagca
    20221 gcgcctgcgg ctcgggcaga atcaccttgg catcgccttc gaggtagccc atcagccgtt
    20281 cacggtccgc cagtgagagg ttgggctgtt cgcgcaccag aatccgtgcc cgctccagcg
    20341 catcgccatc gccctgctcc tgataggcgc ggcgcagctc gacgtagagt ttcgccagct
    20401 tcttgcccat gcgcgccgcc gcggcctcgg cattctcgcg aaagccctgc tcgccgccat
    20461 agaggtgatt gaaacggcgc gccacttcgc gggtcagctc gacatgggcc acctggtcgg
    20521 caccgaccgg caccacgccg gcccggtagg caaggatgtc ggcgctttgc agcaacggat
    20581 agccaaggaa gccataggtg gccagatcct gctccttgag ctgctcctgc tggtccttgt
    20641 aactcggcac ccgctccagc cagcccagcg gggtgatcat cgacagcagc agatgcagtt
    20701 cggcatgttc cggcacctgc gactggacga acagcgttgc cagactcggg ttgacccccg
    20761 ccgccagcca gtcgatcacc atgtcgagcg cgttctgctc gagatccatc tcgctctcgt
    20821 agtgggtggt gagcgcatgc cagtcggcaa cgaagaagta gcactcatac tcctgctgca
    20881 agcgggccca ggtgcgcaca ttgccatagt ggcccagatg cagttttcca gtcggtcgca
    20941 tgccggacag cacgcggcgc cgcgattcca tgatgctcaa agagttctct cccgattcaa
    21001 aaccgattcc tccgcaagcg gcggagtata gcctgtcgcc ggccgctttc agtcggcctg
    21061 catgtcggca aagggtgccg gatcgccctt gcccagtcgc accacctgcg gcgcctcacc
    21121 ggtcagatcg accacggtgc tgggctccag cccactgaag ccgccatcga tcaccagatc
    21181 gagctggtgc tgcagcagtt gccgaatctc atggggatcg gtcatcggct cgctctcgcc
    21241 cggcaggatc aggctggtgc tcatcagcgg ctcacccagc tcctcgagca gcgcctgtac
    21301 aatccgatgc tccggcaccc gaatgccgat ggtcttgcgc ttggggtgct gcaaccgccg
    21361 tggcacctca ccggtcgccg gcaggatgaa ggtgtaggcc cccggcgtgt agcgtttcag
    21421 caggcgaaac cggatgttgt cgagcttggc gtaggtcgat atctcggtca gatcacgaca
    21481 gaccagcgtg aagttgtgcc gttcatccaa tcggcgaatc gcgcggatgc ggtcggcacc
    21541 ggccttgttc tccagccggc agcccagcgc ataggccgaa tcggtggggt aggcaatcac
    21601 gccctccttg cgcagaatcg ccaccgcctg ctgcaacagg cgcggctgcg gattctgcgg
    21661 atggatctga aagaactgac tcatgcgcaa actccgtaag gttggcggag ccgttgatgc
    21721 tcccagatcg gtgcaatgtc gacaggcagt ggcagcgagc gccccagatc ggcccagggc
    21781 gtccgctcgt gatgaaagtc gctgcccacc gatccaccca gccgatgctg gcgtgccagc
    21841 gcactccaga gccgctgggc atccggcgat tgtccggcca tgcccagctc cagcgcctca
    21901 ccgccccagc cgctgaaatc gctcaccagt gccagccgct gcaaccgccc cagccggtag
    21961 tggtgcggat gggccagcac cgccacccca cccgctgcct tcaccaccgc gatggcgtcg
    22021 cccagatggg gccagtgggt gacctcctcc tgccattttt cattgccgat gtggcggtcg
    22081 aacgcctgct ggaaggtgtc gacatggccg ctcgccacca gccagttggc aaagtggacc
    22141 cggcccggcg cggcactgcc cgccagcgcg gtcgcgccca agagagcgcc ttcgatgccg
    22201 cgctggcgca gatgatcggc caggatctcg gcacgccgca gccgcccacg ccgcaactgc
    22261 tcgatcaatc gcaacaacgg ctcatggtcg atatcgatgc ccagaccgat cagatgcagc
    22321 gcgcagcgcc cccaatggca ggagatctcg atgccgggca ccaccctgat tgcctcacca
    22381 tgctgttctg ccgccgctgt ggcctcggcc accccggcaa cggtgtcatg gtcggtcaat
    22441 gccagcagcg tcacgccagc ccgatgtgcc cgctcgacca gcgccgtcgg cgagagcgca
    22501 ccgtcggaag ccgtgctgtg gctgtgcaga tcgactttca tcggcgggac ggttcgctca
    22561 ctttcattca atggatcggc ggattgcagc cggcatcgac agcttctaga ctgtcagcaa
    22621 gcccctgatg gctgcaatgg taacacccct gcccccctct atctgccgaa ccaaccatga
    22681 aacaactgct cgaattcatc cccctgctcg ccttctttgc cgcctacaag ctgtacgaca
    22741 tctatatcgc caccgccgtg ctgatcatcg ccacctcgct ccactacggc attctctggc
    22801 tggtcgagcg caaactcgac aagacccagc gctggacctg gatcggcgtc gtgctgttcg
    22861 gcggtctgac cctggccctc ggtgatgaca cctacatcaa atggaaggcg ccggtgctgg
    22921 actggctctt tgcactggcc tttctcggca gccaactgtt cggtgaaaag tcgctggtcg
    22981 agcggctgat gggcaacgcc gtcagcctgc ccaaaacgat ctggaaccgc ctcaacctga
    23041 gctgggtgat cttctttgcc gccaccggct ttgccaacct ctatgtcgtg ttctatcacc
    23101 ccgaattctg ggtcgatttc aaggtgtttg gcagcttggg actgaccctg ctgttcatgg
    23161 tcggtcaggc gatctacctg gtgcgccaca tcacgcccga agctgtcgaa gagcagggca
    23221 aacagtgagc atgctctacg ccatcatcgc cgaggatgtg ccgcacagcc tgccgctgcg
    23281 gcaaacggcc cggcccgctc acctggcgcg gctgcaggca ctgcaacagc aggggcggct
    23341 gctgctcgcc ggtccccatc ccgccatcga ccaccccgat cctggcgaag ccggtttcag
    23401 cggcagcctg atcattgccg aattcgactc gctggccgcc gcgcagcagt gggcgcaggc
    23461 cgacccctat gtcagcgccg gcgtcttcgc ccacgtcacg gtcaaaccct tcaagcagac
    23521 gttgccctga acaaaaaccg gctgcttttc agaaaatatt caggcgtttt cggctcatac
    23581 cgttaaaacc cgccatggct gcgagtagaa tgccgccacc tcaatcggcc aaggagaccc
    23641 tggtggtgcc cgctttcaga ccccttcgac tgctgccacc catgctgacg ctcctccttg
    23701 ccaccgcaag ccatgccgaa acccgctatg tcaccgatca gtggcgcttc gagctgcgca
    23761 gcggcaacag caatgagcat cgcatcatcg actacctcgg cagcggcacc gccgtcgagg
    23821 tgctcgagcc ggtcagcaac ggcttcattc acatcagcac cgccaagggt gtcgagggct
    23881 ggatgcgcga gagctacctg cagaccgcac tgccggccac actgcggctc gagcagctca
    23941 ccaactcagc caccgctgcg gcggtcgccg cgggcaagct ccccgcaacc agacccctcg
    24001 ccccttcggc gcgcgaaacc gaactggaaa cagccaatca gagcctgacc caacagctga
    24061 ccgaggccaa ggcgctttgc gagaatgccg cccaactcga ccagcgcaac cgcgaactgc
    24121 tgcaggagaa tcaactgctg caggatcaga gcacgctgct caaggtcgaa agcgagcggt
    24181 tgcgggagca actggagaaa aaagagttct atctgggtgc cggcgcgatt ctgcttggcc
    24241 tgctgctggg ggtcatactc ccccatctga agccaaagag aaaatattcg gagtggcgct
    24301 gaacggctgc acttgatcgc cgccacgcgc ctgcgcggca ggtgaccacc atctcaaagc
    24361 acacgcaaaa atttcaggct ccacagggtg ccccgtccac tgctgcgcag ctcgatcacc
    24421 ccctccaact gctccatcaa ctgcttgacg atgctcaagc ccaatccttg gtgcggcggc
    24481 gccttgcggg tcggtatcgg cttgaacagg ttggacagca cctcatcggc catccccggc
    24541 ccctgatcgc gaatggtcaa ttcgacatag tgcttgccat cgacgaggac ctggccgtag
    24601 gtggtgagcg tgatctcgcc ctggaacgcc gaggcatcgg ctgcattgtc gagcaagttg
    24661 atcagcacct gccgcaatag cagtggattg ccccgaatct gcggattggc ttcatccaga
    24721 tcgagccgat aacgaacgtc tttgcccgaa gtgtcagcct gttgcagtgc ctgcaccagc
    24781 tcgcacacca cttcgttgag atcgaacgcc tgccatgcca ttgcagtcgg atgttcttcg
    24841 gcacgctgca cgatgctgtc gaccagctcg accacacggt cgatctcggc ctcgaccggc
    24901 agcagatcgg tcctggtcaa tccactgtgc tgaaaccgca gggcgatgat cgaaaggtag
    24961 tttctggcga tgctgagcgg actgccgagc tcatgccgca actcccgcaa cagcagcgat
    25021 gtctgtccac gcagtgcctg tcgctgcttc actgtctgcg cctgatgcat ccgtatcgcc
    25081 ccaaggcgac gccccagcaa caggccgtag taacgcagtg ccggcagacg tttctccagc
    25141 cggtcgcgtg tgctgatttc cgtcccgatc accagcagtg cgccggctgt cgcattctgc
    25201 atcagcggca gacagaagat cgccgtggcg tccaactccc gaatcagttg ccggtcgagc
    25261 agggccagtg gcgcctccca ctgccaactc cccagcgcaa caccctgagc gaggcagtgc
    25321 gccaccatcg aatcgccatg cccggcagca agagtgatct cgtcagacag catgccgtcg
    25381 tcgtgcgccg ctggacagag cagctcaagg ctgccgtcag tgcacaggta gagcatgaag
    25441 cgctcgacac cggccagcag ccgcagctcc tgtgacacgg cgctcgccaa ggtctgcaag
    25501 tccgcgtcgg gctgccatgc ccagggggtg acgatgtcct tgaccagtgc ctgatcgcgc
    25561 aaggtggcgg ccagactcaa cgccacggca tcatcagagg ctccccagca ggaggaagag
    25621 agtggccaac cagaccaatc tgggctctcc gccaaccgcg tcagaaatgc tgccggcagc
    25681 tcgagccgtt gcccgaccac agtcacggcc tcgatcgcct tcctgttggc gtgccgcaac
    25741 tcttcgctcg gcagggccag attggccgcg atttcgtcga gaccgacact ctgcgcgggc
    25801 agcagaggcg tgatcgcaac cggataggcg tcgaccgcag cgcgcagcag cctgatcagc
    25861 gggtgagcat tgcgggttcg ggagaggggt tcatcaagaa aatggagcgc atcaaccacc
    25921 cgccttggca cccccaacag ggtggtcagc agatcaagct gtctggagcg ctccagatcc
    25981 ggatcttcga gcgccgatgc agtggcgaaa tccgcatcga tggttgccgc cagctcgcgc
    26041 agctccatca agcccagcaa cagcgttccc aggcgcaggt catccagttc aggataggca
    26101 aggtggttcg ccagctcgac agcaaaggtc cgacgcagcg tgatgcgggc ccacagatcg
    26161 agccagcctg cgtcacccaa ctgattgaac acagagaaga actggctggt gaccgcctgg
    26221 tcgagcagag cctcgattcg ccactgaccc agcgcggcaa cgcaatcgcc aagccggtca
    26281 tccgaactct cggcggttgg cgccctgccc tgcagcgcca ggactctggc gctcagcacc
    26341 acatcggact caagcagccg cgtccactgt gtctgcgcgt cgaccgcacc atccagtttc
    26401 cgccgctgca gatgaagcag cagctgtggc agcaccggca agcgcagcca tggccatgcc
    26461 gatgagcgca ggacagcagt ttctagcatg ccatccgcac cggcacagct tgagaaactt
    26521 caagacaatt gagtgaacag cacgccaatg aattgttttt tttggttaag gtgcgcaaaa
    26581 tcagacatct ccgccccagt ttcgcagggg catcgccctc tgcgtccagg acccatcgcg
    26641 tggccagact tccagcactg atcggcatga tgggcgctca ccatggcgct ggcgttacga
    26701 cgctcagctt caaccttgcc gcagccctgc ttgcgctgga taagagtgtc gagattatag
    26761 agggggagct gggttcgaca acagccactc atcggctggc agcggcacgc cgccagagcg
    26821 tgcagcagca ctctggcatc tcaccgatca ttcatcagct gcaggaggcc acccagcttc
    26881 cggcgctgct gcgccgtctg atcgagccaa cccaacggca aagtgccatc gacttcatcc
    26941 tgatcgatct gcccagcctc gacatcacgg ccgactccgc ccagggcgag attctcgaac
    27001 tgctcgatca actgattctg gtcaccactg cgcaacgcag ttcgttccat gcaacgaccg
    27061 agagactgtg gcggttggcc aatcaggaga ttcgcctgcc catcggactg ctgatcaatc
    27121 gggtcaaggg ctaccacgag agccagcact gcttccagcg gctgcaacag gaggtgacgc
    27181 cagcgctgcg cagcgccttc gattacctgg gttgcatcac ctgcgacaat ggcatcgacc
    27241 tggccatgac gctgggcaag ccagtcgcgc tggcctttcc cgacagcccg gccgcactga
    27301 atctgcgctt tctggtctca cacaaacttc agcatctgct ctcgaccggc cgcatcgatc
    27361 ggctgctctg gagcgtcgat cccatgcagt cgcagccact tccagccatg cctgtcgcgg
    27421 caaccgcacc tcaaggcacc acggcagaac ggcaaccgtt gaccgcaccc gacgaaatcg
    27481 aggccgaacc gagccggcca aacagccacg gcgagcagtc ggcactgcat cgcgccctgc
    27541 gtctggcggc cgcgctggtc tgatccattg ccactgcggt gcgctgcaat ccactactgc
    27601 cgggcaagac gcaggttgag accggcgctc tgctgcgtcg aacgccagca gttgatctca
    27661 aggccacctc ggcgcagata ccaggtggca accgtcagcc gccgcggccg gcaatggttc
    27721 aggatgtcga tgaagatctg ctcggtacag gcttcgtgaa agccactgtg gcagcggtag
    27781 gagatcagat aggcgagcaa tcctgaccga tcgatcgaca ccccctgata atcgatcagc
    27841 accgagcccc agtcaggctg gccggtcacc ggacagttcg atcgcagcag gtggctgtgc
    27901 aactgttcga ccacctcatc tcccgccacc cgcagcagct ccgggcgcgg caggtagtcg
    27961 gagatcgcca cctccagctc atcgagcgag ctccccggca gtggttgcca gtcgggatgg
    28021 taactcgcca gcgactgcaa ccgcacccgc acttcgccgc ccagccgcgc tgtcagatcg
    28081 gcggcgatgc gctgctcgac ctcggccatc gcggcgaaac attcctgatt cagcgagttg
    28141 agatagagct tgagcgattt ggactccacc agattgggca gcccggccgc gaaggtcagc
    28201 tcgcccaccg ccaccatcgg cttgccactc ggcatcagcc aggagatctc gtaggcgcgc
    28261 cagcgatcgg caccgaaaaa tggccatgct ccctcattca agccatacgg caggcgcccg
    28321 gcctggcgcg gaatcgcttc cagcaactca ggcgcatagc gttgcggatg ggccacctgc
    28381 ctgcccagca ggctgccatg ttcgctcatt ctgaccactc cagtgccgac agcacgacag
    28441 attgctctac aatttcagaa gcgttcgccc accacatcag ccagacccca ttgatcttcc
    28501 cgcagcctgc cattatccac ccttctccaa cactgtcgca tcctgtaccg agaggtctca
    28561 gaggcggctt cggcctgatt ctgctcattc tgctgctgct cggctgtcag gagccccaac
    28621 cggccgaccc agccacccag atcgacaccg cgcggcagaa gtggcaggaa ggggaggtcg
    28681 atgcggcccg caacctgctg caacagctgc tgacccgcta tccgcgcctc tccccggccc
    28741 gcaccctgct cggtcgcatc gaactggcgc agggcaatct cgaccgcgcc gagcaccatc
    28801 tgcgccatga ccttggctgc gtcgatgacg gcgatcaggc ccggctggcc tatgccgaac
    28861 tgctgcgggc ccggggcgat cttcagcagc tctcgctgct gctggcggcc gatctgctct
    28921 cacccgctgc cgacaacagc gcacgtcatc acttcctgct ggcagaggtc gccagcggtc
    28981 gcggcgactg ggccagtgcg ctcgaccact atcgacgcgc catccagatc gatccacgaa
    29041 tgtccgaagc gcaactgggg ctgctgcgca cgctgatccg ccagggacag gatgccgagg
    29101 cactgcaaca gatcaccctg ctgctgaatg agcccgccgc gccggccgaa ctgttcgaac
    29161 tgcgtgcaga gatcgaggcg cgacagggcc gactcgagac cgcggtacag agcctggaga
    29221 tggccattgc gctggcgccc aatcgagcgg aggccaggat cgcacatgca cgcctgcggt
    29281 tgcgcatgcg gcaacccgat ctcgcccgcc aggagatcgc cgtgctgctg aagcagatgc
    29341 cggattccat tgccgtgcgc ctgctcgatg ccgacctgca tgaacagctc ggcgaccgcg
    29401 acggcaccat cagccggctg cgagagatct ttctgctcga ccagaaccag tccgacgcgc
    29461 tcgggcggct cggcaccctg ctctaccagc ggcaacgctg gcgcgaggcc gagatctatc
    29521 tcgaccgctg gctgacgctc tcccagcgtg gcagcacgga cgaagtggcg atcaggcaga
    29581 tgctggcgcg cagccagttg cagttgcgca acttgaccgc agccgaaaag aatctgctct
    29641 ggctgcgcac gcatggcggc ggcgatgcca gcgaagtcga tctggtgaaa ctctatctgt
    29701 tgggaggaag aacctccgag ggcgtgtcac tggccaccgc cctgaccgag cggctgccac
    29761 aaaacgatgc gccactggcg aaccagctgg ccgaactgct gctgcaacag aatcaaacgc
    29821 aacccgcgct gaagctgctg agccgcatca cttcgaccca gccgcaaacg ccgccaccaa
    29881 cccgattgct gctgattcgt acactgatcg ccagtcagga tctcgaccgc gcccgacgcg
    29941 agctcgaaag ctggcatcag cgcgacaccg catcggcgct cgcctggcaa gccgccggcg
    30001 aggttgcggc agccaggggc cagtggcccg aggcgcagag tgcctggcag cgcgccagag
    30061 ccatcgatga cagcgacccc cgactgtggc tcgacatcgc caccgcagcc cggatgcaga
    30121 accgcgccga cgaggggcgc agactgctcg atgaggccct ggtgcgtttt ccggatcaac
    30181 tggagctgca actgaccgct gccgactttg aacggatgac ccaccatccg gagaattatc
    30241 acagccggat gaaacagctg atcgaacgac atcccgaaga ggcagtgcca cgcatccgct
    30301 acgcccgcac cctgcttgag cttggacagg ccgaggccgc gcagctctgg ttggtgccac
    30361 tggagaccca cgaagaacgc aacgccgact accacctgat tgccgcccag gtcgcgctga
    30421 agcttgccga tgccaacagc gcggtgattc actgcaagcg gctcacccaa cttcagccca
    30481 acagtgcggc ccatcagcag ttgctgggtg acgcctatct gctgcagaac gaaccgctct
    30541 cggcagccag tgcctatgag cgggcctggc agatcgaacc ggcgaacggt ctcgccttca
    30601 ctgcgatgat ccagacccag ctcagactgc aaaaacatgc catggcgcag cgcgccatcc
    30661 tgcgggccag caaacagccg accgggctcg acgcacagac gttggccagc gccgccgcga
    30721 cagtcgccga ggcacagctc gaagcgggcc aatctgccga ggccgaacaa ctgcttgaac
    30781 aactgcccaa actgaccctg accacgcccc ggctgctcgc gctcaggatc gaactgctga
    30841 tcgccagcaa cgcagcggcc gaagccgaac ggctgttatc gctgctgcgg cagatcgagc
    30901 aggaggggcc ctacagccat ttcgcccagg ccagactgct gcaacagcgg ggtcgacagg
    30961 ccgaagccat tgcagagttg caacagtcgc tggcgcgcgt gccgctgcca cgtgcccacc
    31021 ttgccctgct cgacctgctg ctcgaacgca acgcctacac cgaagccgag gcccaggcca
    31081 ctgcctggca acaccagcat cccgacgatg ccagcaacct gcagcggctg gccagactct
    31141 ctcagcacaa tggcaacagc gagcaggcca ttgcccagta ccaggcgctg ctgaaactgc
    31201 aacccggcca gatcgatgcg atggccaacc tgatccgcct gctcgaaccg agcgatccgg
    31261 ccctggcgct tggctacgcc aagcaggcct atctgctcaa acccggttcg gccgagatct
    31321 cgggtctcta tggctggctg ctgctgcgca ccagacagtc accggaccag gcccagcgcc
    31381 tgctgctgag cgcacaccag agccagaccg atgattcaga gattctctat cacctggcac
    31441 aggcggaact ggctctggag cgcaaggccg aagcgatcaa acacctgcgt gttctggcgg
    31501 agcgcgaaaa cgacccctat cgggagcagg ccggcgcact gctcgacagc ctggcctggc
    31561 gaccctcgca gcccaactga acgacagccc ggtcagattg gctttttgcc gacggctcgg
    31621 ctttaatagt tgcccttcgt caattcatag actcgacaac gatcgccatg ccccactccc
    31681 tggaaaacat caacgtcatc tcgcaggatg tgctgctgac cccggtgcaa ctgaagcgcg
    31741 aaattcccgc ctccgaactg gcccagtcga cggtcgcggc cggtcgacag acgctctgca
    31801 acatcctcga tggcaaggac catcgcctct tcgtcgtcgt cggaccctgt tcgattcatg
    31861 acctggatgc cgcccgcgaa tacgctggca agctgcggca actggcccgg caggtcgagg
    31921 atacgctggt gctggtgatg cgggtctact tcgaaaaacc gcgcaccacc gtcggctgga
    31981 aagggctgat caacgacccc tacatggatg actcgttccg catcgacgaa ggattgcgca
    32041 tgggccgcag gctgctggtc gagctgggcg aactcggact gccgaccgcc accgaggcgc
    32101 tcgacccgat cacgccgcag tacctgcagg atctgatcgc ctggtcggcg atcggtgccc
    32161 gcaccaccga atcgcagacc caccgtgaaa tgtccagcgg tctgtcgatg ccgatcggct
    32221 tcaagaacgg caccgatggt ggcctcgaag tggccatcaa tgcactgaag tcggtcaagc
    32281 atccgcacag cttcctcggc atcaaccccg aggggcaggt gtcggtgatc cgcacccggg
    32341 gcaaccgcta tggtcacatc gtgctgcgcg gcggcaacga ccggcccaac tacgactcgg
    32401 tctgcatcgc catctgcgaa cagcaactgg caaaagccag gattcccgcc aacatcatgg
    32461 tcgattgcag ccacgccaac tccaacaagg atccggcact gcaaccgctg gtgctcgaag
    32521 acatcaccca ccagattcag gagggcaacc agtcgatcat cggcgtcatg ctggagagca
    32581 atctggagtg gggcaaccag ccgattccgg ctgatctgac ccagttgaag cggggggttt
    32641 cggtcaccga tgcctgcgtc gactggaaga ccaccgaaga agcgctgctg ctgatgcgcc
    32701 gtcgcctggt cgaccacctg ccgcaacgcc gtcgcagcct cggcagctga tcaaccgcga
    32761 cgctgcgcgc tcccctcgtc cgcatggcga aggggagcgc gcaaccgcat caacccgcgg
    32821 cggtgtgcgc ggtcctcccc cccttgcggc ccgatgccga gacctcgacc acgccggctg
    32881 ccaccagctg atcgacctcg gccgggtcca tgcccagcag ctgttccagc acatagaggc
    32941 tgtcctcgcc gacacagggc gatgcgcggc tgagcagggc cggagtctgc gacagcaccg
    33001 cctgactgcc gttgtagggc acccgcccca tcaccgagtg ctccaactcg acgaagtaac
    33061 cgcgatagag cacctggggg tcttcataga gatcggactg cgactgcacc ggtgcggcgg
    33121 ccacctccgg ctgcagcagg tacatcaggg cgcgaccatc ctgatcacgg gtccagtcac
    33181 cgaggtgctg atcgagcagc gcctgatgcg tcagccggcc gctcagcgtc tgaaaacggt
    33241 catcctcggc ccaggccggc gcgcccatc
//
LOCUS       DAORCQ010000018        33058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_705_length_33058_cov_5.523377, whole genome shotgun sequence.
ACCESSION   DAORCQ010000018 DAORCQ010000000
VERSION     DAORCQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 33058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33058
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_33058_cov_5.523377"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PKI23_03150"
     CDS             <1..1204
                     /locus_tag="PKI23_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNN35970.1"
                     /translation="LQPADAPMASSGQGVPYELVMEPTGKRWIFALDHSSSGDSTIRK
                     LTDGRLQSSTPISERRRFALRRQTDTAPATLSVQQRQRYLQLPARGNPRSRKLAEQWR
                     AQNPAAEAVVRQVLDHFHDQPFHYTLEAPLLGDQPIDQFLFETRSGYCEHYASALTFL
                     LRAAGLPARIVAGYHGGELNPIDRYLIVRQSDAHAWVEVWSDLAMTWLRVDPTAAIAP
                     ERVSQSAVRAAPAGESLTAGPEAGWRPAAWLHWLQFFRDAAEYRWHRTVLSYDLSRQQ
                     QWLGQWFEGVDGVTLARATLLVLLLAMLLPLFWLLPGRNGPHLDPADRSYQRFCRRLT
                     SAGLARRPGEPPADYARRLVLMRPDLAAEVNRISRAYMVLRYGQPTARRAARLDRVLR
                     GAVRRFRV"
     gene            1446..2705
                     /locus_tag="PKI23_03155"
     CDS             1446..2705
                     /locus_tag="PKI23_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNN35971.1"
                     /translation="MFCTPLAHASELNQGNSGWMLTATALVLFMTIPGLALFYGGLVR
                     SKNVLSVLMQCFAITCGASLLWLVCGYSLAFTDGGALNDWIGGSAQFLLASLSEAALS
                     GSIPESVFVMFQMTFAIITPALVVGGFAERVKFSAMLLFSMLWLLLVYVPVAHWVWGG
                     GWLAKLGLLDFAGGTVVHITAGVAALITALVVGNRRGFGTQAMPPHNMTMTVTGAGML
                     WVGWFGFNAGSALAADGNAGMAMLATHISAATGALAWMLMEWVKHGKPSMLGLVTGMV
                     AGLGTVTPASGYIGPGGALLIGFIAGIVCYYMTQLLKQKLKIDDSLDVFPVHGVGGIL
                     GTLLAGLFASDQLGLFSGQGYAEGRTMASQLTIQFIGVVTTLGYTALTTYLLLKLVDW
                     LVGMRVESDEETMGLDLALHDERGYDL"
     gene            2737..4080
                     /locus_tag="PKI23_03160"
     CDS             2737..4080
                     /locus_tag="PKI23_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNN35972.1"
                     /translation="MKEAENSPAAIRPTLGVVALTVYGVGNMLGAGIYGLVGKAAAEL
                     GNLVWLGFVTSMVAAGLTGLSYASLGSRYPRAGGAAYVTQRAFGLPAISYVVGLAVMM
                     SGITSMAAASQVFAGYTHEIFTAIPIKAVVLLFLLALTVINYVGMRESAWVNALCTVV
                     EAGGLLLVIVVGFRYWGSVDYLDATSLKNPGGDFSVPMVLSCAVLTFYAFIGFEDLLN
                     ISEEVREPRRIFHIALLCALGIATLIYIAVSITAVSVVPHAQLAVETGPLVAVVRVAA
                     PWVPPLLFTVVAMFAVTNTALLNSIMGSRLAYGMAHQGLLPKLLGRLHVRRQTPHVAI
                     LLLMAIVLGLALVGDIRTLASATSLLLLGSFTLVNLSLLRLQRRADEPKGGFEVPQWV
                     PAAGAGISLLFIINRVIEGVRASDLTPVYIAGGIIVAIILLYLLVRPQAVADIDG"
     gene            complement(4138..4452)
                     /locus_tag="PKI23_03165"
     CDS             complement(4138..4452)
                     /locus_tag="PKI23_03165"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNN35973.1"
                     /translation="MSKFENVSVVKQANVYFDGKVTSRSIHFSDGSMKTLGFMLPGEY
                     RFNTGDRELMEIMSGKLDLQLSGSSEWRPITAGQSFEVPGASSFTVRVSEPTDYCCSF
                     IK"
     gene            4664..6355
                     /locus_tag="PKI23_03170"
     CDS             4664..6355
                     /locus_tag="PKI23_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNN35974.1"
                     /translation="MPLSIEEANQRLIAASGGMFDIEEINIDGVMTKVWKSAPPTLRH
                     VFLNTKNYFGRDYFLYENERMSYEQHYQRVVALAWQLRSRFGIEKGDRVALSMRNYPE
                     WPVVFWATVTLGAVIVPLNAWWKGNELEYGLADSGSKVLFADGERIELLKEHFTKLPE
                     LKHVIAARTTAPLPAGMLRYEELFATAAPANELPEADIHPDDNATIFYTSGTTGNPKG
                     ALGSHRNMCSNLISGAFVRMRTEIRYGRVPAPPPGPSGHLLSVPLFHATGCHGILCGN
                     TFAGNMIVTLYKWNPEEAIRLIEREKLASFGGVPSMVWQVIESPEFAKHDLSSVQSIG
                     YGGAPSAPELVKRIGESFPQVKPSNGYGLTETSALTTSNSAEDYLEKPTSAGCPAPVS
                     EVRIAGDDGQEVPRGTIGEVWIKGPQVVKGYWRKPEATAQSFTDGWLHTGDLGYMDGD
                     GFLYIADRAKDMLIRGGENVYCVEVESALYSHPDVMDAAVVGIPHRVLGEEVGALVQI
                     RPGGNPTEEELKKHVASQLAGFKVPVRIDMQRDPLPRNANGKIMKPEVKKLMGLA"
     gene            6413..7246
                     /locus_tag="PKI23_03175"
     CDS             6413..7246
                     /locus_tag="PKI23_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN35975.1"
                     /translation="MSDFTPGEAVAVAPNVLRLTAPNPGVMTGPGTNSYLVGTKAVAV
                     IDPGPADAGHVAALLAAAPGPIRWILVTHTHLDHSPAAALLREKSGAQLVGMPAPEHG
                     AQDKKFKPGRIMIQDERLVGDDFVLTAIHTPGHASNHLCYLLEEEGLLFTGDHIMSGS
                     TVVIAPPDGDMASYLASLALLKRYPLKRLAPAHGGILEQPEAVIDWLIAHRLGREAKV
                     VEALAAAPDSTLDELLPVVYQDVQALLFPVAKHSLTAHLLKLKDEGRARRSDNGRWRN
                     C"
     gene            complement(7247..7543)
                     /locus_tag="PKI23_03180"
     CDS             complement(7247..7543)
                     /locus_tag="PKI23_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35976.1"
                     /translation="MKHLVGGWIGALLGCIALLGIDHWLRSNGHFTGLGDDTMKLGLA
                     LIAAAAAIAWYRGLAGQPLPLRIVLVGLQLAAGYLITLGLAIAYSCMAGLGCIA"
     gene            7773..9827
                     /locus_tag="PKI23_03185"
     CDS             7773..9827
                     /locus_tag="PKI23_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNN35977.1"
                     /translation="MSRLLTPGINLMKKMNFLYKFGLISLAVLVPLMLLSALLVMQTS
                     RDIGTIRAERVGIEHAKVALELRRLSGRYVDLVLAIEQVNKQMSAANDLTPLYRQTRQ
                     QIDEAIKAYQAVLEQQPLGEETQSSWTNLLNFWQQSAQIDPKAGVEVPQHLAHAEQKK
                     LLELMQHFILAVSIESGLILDSSPERYLLISHVLNEFTMLAGRLEEARAVGTSIKAQT
                     ATTASTLKWLEPIGQKLLDTEEEYHYAVATSIGRSPRAKALLEADANKAGEAYKGMAE
                     YIESVLMMGEEQSLTWEGYYNHLSAKIDPIFKVGQSTLPLVDTMLVEQESALIAQRNG
                     YLITALVVLALVVYLCAAFASSMKVTISSIYAAARGLAQGDTRVRVAVDSKDELGSLS
                     VEFNSMAEKVAELISQAHHTARQVSEQAETVERIASRSSDELQSQLSETAQVATAMNE
                     MAATVQEVARSSVSAADSAQAAHRDVQSGRQLVESTSATIRRLAEEIGGTVQVTQRLV
                     EHSHSISKVVDVIKGVAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLAQRTQQS
                     TSEIEQMIGQLQTGVNDAVKTMGSSREMTERTVVESSKVDEALASIAGSVDNILGISQ
                     QIATAAEEQSVVAREIDRNIVAINGASEHTASGARETVEASQNMAALTQQLEQLIGTF
                     KV"
     gene            complement(9903..10340)
                     /locus_tag="PKI23_03190"
     CDS             complement(9903..10340)
                     /locus_tag="PKI23_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HNN35978.1"
                     /translation="MNDNDLILASFEQTADKAGDISAAVYERYFAASPESAHMMEHMD
                     HLMRARMLDEAMMLIMMPDGDELETVLGFEVLTHTNNGVLLPMYSALFDAIRQTVRDA
                     VATAWNADFEGAWQRRIAALTQEINRHYEEVISHQPHAIGSGR"
     gene            complement(10457..11923)
                     /gene="pyk"
                     /locus_tag="PKI23_03195"
     CDS             complement(10457..11923)
                     /gene="pyk"
                     /locus_tag="PKI23_03195"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNN35979.1"
                     /translation="MLRRTKIVATLGPATDAPQVLEQMLLAGVNVVRLNFSHGEAEEH
                     QARARMVREISARHNLNVAILADLQGPKIRVRSFRDGKVTLQRGAPFALDATLDEFEG
                     DIHQVGIDYPALPADCRPGDILLLDDGRIELRVDSITTHRIDTEVLVGGPLSNHKGIN
                     RQGGGLSAPALSDKDEADIVTAAAIGVDFVAVSFVRSAEDIDTARQLIREAGSQAAVV
                     AKIERAEAVASDAALDRIITAADVIMVARGDLGVEVGDWELVGIQKQIIKRAWTLNRV
                     VITATQMMESMIDNPVPTRAEVFDVANAVLDGTDAVMLSAETATGHHPVEAVRAMART
                     CIGAEKQPSARSSSYRVDSEFHRIDEAIAMASMYIANHLTGIKAVVCLTESGATPLWM
                     SRISSAQPIYALSRHHSTLRRVALYRDVEGLLYDYTQVEPDEVNKGAIDLLEQQGAVQ
                     KGDLVIITEGDHRGKPGATNTLRILQVGTTFKGTYRPN"
     gene            12057..13556
                     /locus_tag="PKI23_03200"
     CDS             12057..13556
                     /locus_tag="PKI23_03200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNN35980.1"
                     /translation="MPVFSSLRPVLTMLLCGLLFVNAALRAEPQVPIGQDEPQLPYLG
                     DVSSSIISTDEEHFLGQIWLMQLRSQVTMLDDPVVNHYLENLLGQLAGSSQLQDRRLT
                     LVVIDSPELNAFAVPGGVVGVNAGLFSHARNEAQLASVLAHELAHLSQRHHVRMVDSA
                     RKNSVPMLAAMLAGVVLAASGGGDAGMAMITGSQAALIQEQLRFSRQYESEADRIGML
                     NLVAAGYDPKQMPAMFEQMLQARSFAGDRAPQFLLTHPLTEARIADTQNRVAQYPDNA
                     EAKVDSIEYEQARSRMQNALEGNPVRIIKQVEATLATQPGGALDDGNRYLLALAQLKA
                     QRPQEAEATLKPLLEREPLALGYLYTQVQIDMARGRAQAALARLDPALKRAPDSYPLT
                     IAQAEALLASGRPAAAEPLLARLSRERPWDAPVWYRLAEARGRSGDARGMHRAQGEYA
                     LLMGQIDDAQRQLEYARALSQGNYGESASVDERLGQIRRLRKQLGMATR"
     gene            complement(13558..14616)
                     /gene="nadA"
                     /locus_tag="PKI23_03205"
     CDS             complement(13558..14616)
                     /gene="nadA"
                     /locus_tag="PKI23_03205"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNN35981.1"
                     /translation="MNVTEKLLKSHQIDETLFVEARDGHHLAEAQYQALKRQIEALLA
                     SKNAVIVAHYYTDPQLQRLAEETGGFVSDSLQMARFGKEHPATTLMVVGVRFMGETAK
                     ILTPHKRVLMPTLEATCSLDISCPIDPFSAFCDAHPDHTVVVYANTSAAVKARADWVA
                     TSSMALELIEHLSAEGQKILWAPDKHLGSYLAEQSGADMLLWDGACIVHEEFKAQGIR
                     DLKHVYPDAAVLAHPESPKTVLDLADLVGSTTQIIEAARRLPNDTFIVATDRGLIYKL
                     EQLAPGKRFLEAPTGGRGATCHSCAHCPWMAMNHLENLLVSLRDECNEIELDAELCRQ
                     AMVPLERMLAFSKSLPPR"
     gene            complement(14717..16102)
                     /gene="purB"
                     /locus_tag="PKI23_03210"
     CDS             complement(14717..16102)
                     /gene="purB"
                     /locus_tag="PKI23_03210"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNN35982.1"
                     /translation="MTLSTLTALSPIDGRYADKSTELRRYFSEFGLIRHRLLVEVRWF
                     QHLAQSGQLPELPPLSSAANHFLNSLVDQFDEAAAEQIKQIERGTNHDVKAVEYHLKQ
                     CFASQPELRPRLEFIHFGCTSEDINNLSHALMVQGARNDILLPLIDRLIDTIAEFSVQ
                     LAGLPMLSRTHGQSASPTTVGKEFANVAYRLRRQRQQIATTALLGKMNGAVGNFNAHC
                     VSYPELDWPTLASRFVTDLGLAWNPYTTQIEPHDCLAELFDAIARFNTVLIDFTRDLW
                     GYISLGYFGQKRVAGEVGSSTMPHKVNPIDFENAEGNLGIANALLHHFSSKLPISRWQ
                     RDLTDSTVLRNLGTAFAHTTIALHSTLKGLGKLEINRERIAADLDSAWEVLAEAIQMV
                     MRRHGVEEPYEKLKELTRGQAIDQAALQQFIDQLAIPEAEKARLRQLTPAGYIGNAPH
                     QATSIRQWLDD"
     gene            complement(16152..16775)
                     /gene="hflD"
                     /locus_tag="PKI23_03215"
     CDS             complement(16152..16775)
                     /gene="hflD"
                     /locus_tag="PKI23_03215"
                     /inference="COORDINATES: protein motif:HMM:NF001246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HNN35983.1"
                     /translation="MARSIEEQALAFAALLQFAQGVDRLARTGVVSQSNIETAVTATL
                     CTHPASTEAVFGGLSPILPGLRLLSGWRQNGKSDEMVSTLRYAVAAIHLQGKLAAQPR
                     MLDQIGQELVQIGRQQQLPDFDQAALVGQLSRLYQQSLSTLRFRIQVVGEPRHLQNSE
                     IADKIRLLLFAAVRSAMLWRQLGGRPLFLLWQRRALINTADRLLQRH"
     gene            complement(16776..17921)
                     /gene="mnmA"
                     /locus_tag="PKI23_03220"
     CDS             complement(16776..17921)
                     /gene="mnmA"
                     /locus_tag="PKI23_03220"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011168961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNN35984.1"
                     /translation="MPPSFAQRIVVGMSGGVDSSVAAFLLKQQGHQVVGLFMKNWEED
                     DGTEYCTAKQDLADARQVADQLGIELHTANFAAEYWDQVFSHFLSEYRAGRTPNPDVL
                     CNREIKFKVFAEYAELLGADQIATGHYARLDRQGGQTRLLKGLDANKDQSYFLHAVSA
                     QQFARSHFPVGALQKSQVRQIAAQLGLATQSKKDSTGICFIGERRFRDFLTRYLPAQP
                     GPIETAEGRRLGEHHGLLHHTIGQRQGLGVGGVHGAAEAPWYVAAKDLDRNTLIVVQG
                     HDHPLLLADGLTTSALHWIDGEGPRLPCRLAAKTRYRQPDQPCWLEPAEDGRCRVRFD
                     TPQRAVTPGQYVVFYQDEICLGGGVIETALPVMQPAQPFSPALRHGA"
     gene            complement(17961..18413)
                     /locus_tag="PKI23_03225"
     CDS             complement(17961..18413)
                     /locus_tag="PKI23_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNN35985.1"
                     /translation="MDWYPHATVATIVERDGHFLLVEESEGERLVINQPAGHLDQGET
                     LAAAAVRETLEETGWEVTVKALLGLYTYTSAYNGISYLRTCFIAEPLRHHPERPLDTG
                     ILRTLWLTVDELRARRDRLRSPLVLRNVEDYLRGKRYPLDFIHEHSGQ"
     gene            18518..19009
                     /gene="moaC"
                     /locus_tag="PKI23_03230"
     CDS             18518..19009
                     /gene="moaC"
                     /locus_tag="PKI23_03230"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNN35986.1"
                     /translation="MSQLTHLDADGNARMVDITDKAESSREATAEAIVRMAPDTLRLI
                     TEGRHKKGDVLTVARIAGIQAAKRCAELIPLCHPLLLSAIQVELTPDIAQSRVVIRAT
                     CRLRGQTGVEMEALTAASIAALTIYDMCKGVDRGMVIDGVRLLEKRGGRSGHWQAETP
                     STP"
     gene            19006..19257
                     /gene="moaD"
                     /locus_tag="PKI23_03235"
     CDS             19006..19257
                     /gene="moaD"
                     /locus_tag="PKI23_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNN35987.1"
                     /translation="MKICYFARVREALGLDHELLQPPPQVDRVDRLMAHLIAARGPLW
                     AEVLDPERILVAVNQQIAAFDTPIGADDEVAFFPPMTGG"
     gene            19259..19714
                     /locus_tag="PKI23_03240"
     CDS             19259..19714
                     /locus_tag="PKI23_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNN35988.1"
                     /translation="MFEVRVQQHDFSLEAEVNQLRQQAPGAGAVVAFVGLVRELGEAR
                     QIEQLVLEHYPQMTENSLRQIVEEARQRWPLLAARVVHRVGALTPAEQIVLVTTATVH
                     RHDAFAAAEFIMDFLKTSAPFWKKEQGVSGAHWIDARDSDQRARERWQR"
     gene            complement(19716..20339)
                     /locus_tag="PKI23_03245"
     CDS             complement(19716..20339)
                     /locus_tag="PKI23_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNN35989.1"
                     /translation="MALLILFNKPYGVLSQFSGDGAHPTLKPFIELPNVYPAGRLDRD
                     SEGLLLLTDDGQLQHHIAHPRHKQQKSYWVQVEGEPTTEAIGTLQQGVLLNDGMTLPA
                     QVRRIDEPALWPRQPPIRWRANKPTHWLEITIREGRNRQVRRMTAAVGLPTLRLVRWR
                     IGDWTLDGLAPGEWRSVQIESPAQTAPHPRRVPASRPQRAPKRSPRG"
     gene            20547..21809
                     /gene="icd"
                     /locus_tag="PKI23_03250"
     CDS             20547..21809
                     /gene="icd"
                     /locus_tag="PKI23_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNN35990.1"
                     /translation="MKRTKLVIPKDGEKITANADLSLNVPNHPIITYIEGDGTGVDIT
                     PVMLKVVNAAVDKAYGARRSIAWMEIYAGEKATRVYGPNEWLPAETLEAFREYLVGIK
                     GPLTTPVGGGIRSLNVALRQELDLYVCIRPVRWFKGVPSPVIAPQKTDMVIFRENSED
                     IYAGIEWKADSPEAIKLIRFLREEMGVTQIRFSEHCGIGIKPVSEEGTKRLVRKAIQY
                     AIDCDLPSVTLVHKGNIMKYTEGAFKDWGYELARERFGATPLDGGSWHAFNNPRTGRQ
                     IVVKDVIADAFLQQILMRPEEYSVIATLNLNGDYISDALAAEVGGIGIAPGANLGGSV
                     SVFEATHGTAPKYAGQDKVNPGSLVLSAEMMLRHIGWSEAADLILKGVEGAIEAKTVT
                     YDFERLMPDAKLLRCSEFGDAIIRNMEG"
     gene            complement(21878..23692)
                     /gene="recQ"
                     /locus_tag="PKI23_03255"
     CDS             complement(21878..23692)
                     /gene="recQ"
                     /locus_tag="PKI23_03255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNN35991.1"
                     /translation="MTLAGLDTPLDILTRVFGYHAFRGRQAAIIDHVVGGGDALVIMP
                     TGGGKSLCYQVPALVRDGVGVVVSPLIALMDDQVAALRELGVAAAALHSGLPAGEAAA
                     IERDLLAGRLKVVYMAPERLVGERTLELLSRVPVALIAIDEAHCVSQWGHDFRPEYLG
                     LGVLGERFPGVPRLALTATADGPTQRDIVQRLGLTAAAVFTASFDRPNIRYHIRQGEG
                     LREQLLAFLDAHHRTDSGIVYCPTRRRVEETADWLERHGRPALIYHAGLPAETRTANQ
                     RRFLREDALVMVATVAFGMGIDKPDVRFVAHLGLPKNVEGYYQETGRAGRDGLPANAW
                     LGYSLADVVGTRQLLAASDAPEERRRVEQAKFSALLGLAESTGCRRQALLAYFGEQLP
                     APCGNCDNCLDPPATFDATEAAQLALSCVYRTGQRFGVAYLVEVLRGGDDPRIASFGH
                     DRLALFGRGQERPAAQWRSLFRQLAARGLVSVEIDGHGGLRLTEAARPVLRGEQRLFL
                     REENTARERRRRKGSAAPLLASAEQALFEALRAERRRLADAHGVPAYVIFHDATLAQM
                     AAERPRTLADLAQINGVGAAKLERYGEAFLAVIGGMTP"
     gene            complement(23975..24661)
                     /locus_tag="PKI23_03260"
     CDS             complement(23975..24661)
                     /locus_tag="PKI23_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HNN35992.1"
                     /translation="MTDFFRFPHTPHLAWLGPGVPRDDKVLAPAEAQALLAGEVVVEE
                     KLDGANLGLSLAPDGRLRAQNRGQYLYEPHAGQFARLPAWLAQHEAGLREALTPELIL
                     FGEWCALTHSIAYTALPDWFVGFDIYDRASQRFWSLARRNALLARIDIPAVPEVTRGR
                     LTLAMVKQLLEETPSACGAPHVEGFVLRRDAGDWCEARAKLVRADFVQAIDTHWRRRM
                     PASNRLALTT"
     gene            complement(24671..25204)
                     /locus_tag="PKI23_03265"
     CDS             complement(24671..25204)
                     /locus_tag="PKI23_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35993.1"
                     /translation="MKARYVVDTNVLIAASAADPIHPKDIDATPADPGLRKVIWDWLY
                     AFEQGPSRMVLDLAGRIYDEYINNRNLDFNAYGIQVVMHKWSTAAVDGVEVVYDNDGH
                     GVLPATLASVVHDLADRKMVAAALAAREAFGEGCIAFAGDTDWHDWERALAAHQVQLE
                     PIIEAWSRQKHAEKRHR"
     gene            complement(25201..26487)
                     /locus_tag="PKI23_03270"
     CDS             complement(25201..26487)
                     /locus_tag="PKI23_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HNN35994.1"
                     /translation="MFTRLRLINFKAWRDTGDLALKPVTMLLGTNSSGKSSLIQSLLL
                     LKQTVQSPDRTVHLNLGGDEVNDLFNFGSFDDVLNQAAASPRQFSIAFDFMRDDHERI
                     KQGGFDCSYGQTSSRSVAVQTLKLSTDSRRFQAVRRDKGAFSILVDEESQPRMKGRDC
                     APERSIALSADVIARLDKDGPLAEDLSLAIRRELESISYLGPLRRKPERDYPWNKTKP
                     GDVGSDGRAAIDALLASALLKGEEQNVVVEGVSKWLKRMKVAENLEVRQQGRSNRYEL
                     VIHRDGVACNLRDVGIGVSQVLPVLVVAYFAPRGSTIILEEPEIHLHPLAQSVLAELF
                     VEVSRQRGVQFIVETHSEHLFRRMQTLVARQKVSTDDVALYFVERKGKSAHLRTLALD
                     DFGRLPDWPPGFFGDALGETREQARLMFERQKKSAP"
     gene            complement(26487..26657)
                     /locus_tag="PKI23_03275"
     CDS             complement(26487..26657)
                     /locus_tag="PKI23_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35995.1"
                     /translation="MKTATYFLGPAEVMDADCPSETFRVLKDKEIRQYGEYRTRRLVL
                     AAWDRLETGELR"
     gene            complement(26661..26984)
                     /locus_tag="PKI23_03280"
     CDS             complement(26661..26984)
                     /locus_tag="PKI23_03280"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNN35996.1"
                     /translation="MSFAILLTEDALRDLDDLYAFIAAQDGIELGDGVLERIHEKLTA
                     LRELPERGEYPPELAALDVRQFRQTHYKPRRMVYRVGQDTVTVMLIADGRQDMQTLLQ
                     RRLLG"
     gene            complement(26981..27136)
                     /locus_tag="PKI23_03285"
     CDS             complement(26981..27136)
                     /locus_tag="PKI23_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35997.1"
                     /translation="MVQDIHSYEQTQQTLALLKILALGQQQIAEGRVVPLDDAVARVK
                     ARHQGSE"
     gene            complement(27340..27477)
                     /locus_tag="PKI23_03290"
     CDS             complement(27340..27477)
                     /locus_tag="PKI23_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35998.1"
                     /translation="MPVALPDTPRHLQTEWEDRGYTFVRFGEEHAQWALAFGKLVKLV
                     G"
     gene            27492..27737
                     /locus_tag="PKI23_03295"
     CDS             27492..27737
                     /locus_tag="PKI23_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN35999.1"
                     /translation="MQQRAAGVLGGNRRQQATRFGGEALLPGRLPAFGFIHVCFCAGW
                     RLRRGSLGQRGFGAGQAPQDAAGVFVAPVQQFGSGWL"
     gene            complement(27937..28275)
                     /gene="cspD"
                     /locus_tag="PKI23_03300"
     CDS             complement(27937..28275)
                     /gene="cspD"
                     /locus_tag="PKI23_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008156 - negative regulation of DNA
                     replication [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HNN36000.1"
                     /translation="MSLGHVKWFNNAKGYGFIIADNRSEDMFAHYSAIIMDGYKTLKA
                     GQRVSFEISKGPKGYHAIGIRPLDMLEESSSLPADAAPDLPPARPEMAKTHEHHDTAS
                     QRSLESMAVE"
     gene            complement(28570..30732)
                     /locus_tag="PKI23_03305"
     CDS             complement(28570..30732)
                     /locus_tag="PKI23_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN36001.1"
                     /translation="MSISGKQPDAARLLVVDDEIRLLRSLQELLVLHGYQTELAQGGR
                     QAIERLDNDHFDAILLDLRMEDISGHDVLQHIADQQYETPVIVISGESSFGAITQALL
                     LGATDYIRKPYSSDELLKTLSNTLTNQRLKTENRLFQQRLWQSEKLHRHLVEHSPDVI
                     YVLDHEGRFTFINNRVSDLLGYQTEALLGQHFSVVLDDEEEWQQLSCDTGHGDVESSF
                     RKQAEIRVRNKFHQVRHLYVEYFPLDFSQTGVIDQSISEITSTTQGTFGIARDITERK
                     QAEQTIQFQAYHDLLTGLPNRTLFKDRLEMAVAHAKRNKDIFAVLFIDLDRFKVVNDS
                     LGHATGDLLLQAVSETLRSSLREGDTLARFGGDEFTLLLPSLHSEQDAATIAGKIMAR
                     LASPFLLDGHEIFIGASIGIALYPRDGTSMNQLVGHADLAMYQVKKRNRNGFCFYESS
                     MDAAASERLLLENDIRRGIEGQQFCVQYQPQMDIDSGKILGMEALVRWRHPLRGLLLP
                     NEFIPIAEESGLVLMLGEWVLRQGCAALREWLDAGLEPIRMGINFSVLQIEQPNFTSL
                     VLAVLEEHRLDPSLLEVEITENLLMKDWEGVVAKLQTLHQHGVKIALDDFGTGFSSLS
                     YLQKFPLTTLKIDRVFINEINVTNPDACLVDAIASIGKGLKLNLVAEGVETAEQLDYL
                     RRLGCHEAQGFLLSRSLDPSDARSLLERNQNRATVALS"
     gene            complement(31272..31847)
                     /locus_tag="PKI23_03310"
     CDS             complement(31272..31847)
                     /locus_tag="PKI23_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HNN36002.1"
                     /translation="MNTSDPTVEHLAQQIHHFKPPASLPPVDQWHPARVGEIDIRIAR
                     DGTWYHEGEPIRRKELVRLFSTILRRDEDGHSYLVTPAERLRIEVEDAHFLVTDVEVA
                     GEGDQRRFCFTTQVGDQVLAGPDHPLWVEESATGEPSPYLAIRGRLHGRLTRALFYRL
                     IEMAEERMVDGKPQLGITSSGIFFSLGSATE"
     gene            32192..>33058
                     /locus_tag="PKI23_03315"
     CDS             32192..>33058
                     /locus_tag="PKI23_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN36003.1"
                     /translation="MALDFATRRFLEMMAERGRPPLHAGTPTEARLASAAMADLAGPA
                     PPMVRVEDHSVVSSDGGSVPMRLVVPIESPRGLIVFYHGGGWVIGSAQQSETVGRKLA
                     ERTSCAVLLVDYRLAPEHRYPIAVEDSYAALLWADANLQRIAGRRVPLMVAGDSAGGN
                     LAAVMALRARDQQGPALALQVLIYPVTDADFERASYVDPENQLFLSRESMLWFWDHYL
                     PDPARRLEPDASPLHAADLSGLPPAVVLTAEHDVLRDEGEAYAQRLQQAGVAVDFKRH
                     AGQMHGFFTVLAL"
BASE COUNT         5765 a        10143 c        10968 g         6182 t
ORIGIN      
        1 ccttcagcct gccgacgcac cgatggcctc ttccggacaa ggagtgccct atgagctggt
       61 gatggagccg accggcaagc gctggatctt tgcgctcgat cactcatcga gcggtgattc
      121 gaccatcaga aagcttacgg acggtcggtt gcaaagcagc acgccgatca gtgagcgccg
      181 ccgctttgcg ttgcgacgcc agacagacac cgcgccggca acgctttcgg tgcagcagcg
      241 ccagcgttat ctgcaactgc ccgcgcgcgg caatccgcgc agccggaagc tggctgagca
      301 gtggcgcgcg cagaacccag ccgccgaagc ggtagtcagg caggtgctcg accattttca
      361 tgaccagcct ttccattaca cgcttgaagc gccgctattg ggtgatcagc cgatcgatca
      421 attcctgttt gaaacccgca gcggctactg cgagcactac gccagtgcgc tgacctttct
      481 gctgcgcgcc gctggactgc ccgctcgcat cgtggccggc taccatggtg gtgagttgaa
      541 tccgatcgat cgctatctga tcgtgcgcca gagtgatgcc catgcctggg tcgaggtctg
      601 gagtgatctc gccatgacgt ggctccgggt cgatccgacg gcggccattg cgccggaacg
      661 ggtcagccag agcgcggtgc gcgctgcccc ggcaggcgaa tcgctgaccg ctgggccgga
      721 agctggctgg cgccctgccg cctggctgca ctggctccag tttttccgtg atgccgccga
      781 gtaccgttgg catcgaacgg tattgagcta cgacctgtcc cgtcaacagc agtggctggg
      841 acagtggttc gaaggggtgg atggcgtgac attggcgcgt gccacgctgc tggtgctgct
      901 gctggccatg ctgttgccgc tgttctggct gttgccgggc cggaacgggc cgcacctcga
      961 tcctgccgat cgcagttatc aacgtttctg tcgccgcctg accagtgccg ggctggcgcg
     1021 ccggcccggc gagccgccgg ccgactatgc ccgccgtctt gtgctgatgc gtcccgatct
     1081 tgccgctgag gtcaaccgca tcagccgggc ctacatggtc ctgcgttatg gtcagccgac
     1141 tgcgcgacgg gccgcacggc ttgatcgggt gctgcggggg gctgtgcgtc gattcagggt
     1201 ttgacgcgcc agcccagggt tgtgttgccg aggtttcgca cagaaatggc gcaatgcttg
     1261 ctttaaccgc acgcagatcc ggttttgcct caaaaatgca tttttatggt gccaatccgg
     1321 atttgttccc gttatgacca ataaaaaagc accgtttttg tgcgttttta cgtacttgag
     1381 gatccgatcc catgtcagca gccactgcca gaccccccgt ctttttgacc gtactgttgt
     1441 cattgatgtt ctgcacgccg ttggcgcatg ccagtgaact gaatcagggc aacagcggct
     1501 ggatgctgac agccaccgcg ctggtgctgt tcatgaccat tccggggctg gcactcttct
     1561 atggcggtct ggtgcgctcg aagaatgtgc tgtcggtgct gatgcagtgc tttgccatca
     1621 cctgtggggc ctcgctgctc tggctcgtat gtggctacag cctcgccttc accgatggtg
     1681 gtgccctcaa tgactggatc ggtggttcgg ctcagtttct gctggccagt ctgagcgaag
     1741 cagcgctgtc gggctcgatt cccgagagcg tgttcgtgat gttccagatg accttcgcca
     1801 tcatcacccc ggccttggtg gtgggtggat ttgccgaacg ggtgaagttt tcggcgatgc
     1861 tgctgttcag catgctctgg ctgctgctgg tctatgtgcc ggtggcccac tgggtctggg
     1921 gcggtggctg gctggcgaag ctgggattgc tcgatttcgc cggtggcacc gtggtgcaca
     1981 tcactgctgg agtggccgcg ctgatcaccg cgctggtggt cggcaaccgc cgcggttttg
     2041 gaacccaggc catgccgcca cacaacatga cgatgacggt gaccggtgcc gggatgctct
     2101 gggtgggctg gttcggcttc aatgccggat cggcgctggc ggccgatggc aacgccggca
     2161 tggcgatgct ggcgacccac atctccgccg cgaccggggc cctcgcctgg atgctgatgg
     2221 agtgggtgaa gcatggcaaa ccctcgatgc tgggcctggt gaccggaatg gtggctggcc
     2281 tgggcacggt gacgccggcg tccggttaca tcggaccggg tggtgcgctg ctgatcggtt
     2341 tcatagccgg catcgtctgt tactacatga cccagctgct caagcagaag ctgaagatcg
     2401 atgattcgct cgacgttttt ccggtgcatg gcgttggtgg tattctcggc acgctgctgg
     2461 cgggcctgtt tgcttccgat cagttgggtc tgttcagcgg tcagggctat gccgagggca
     2521 gaacgatggc cagccagctg accattcagt tcattggcgt cgtgacaacc ctcggttaca
     2581 ccgcgctgac cacctatctg ctgttgaagc tggtcgattg gctggtcgga atgcgggtcg
     2641 agagtgatga agagaccatg gggctcgacc tcgccctgca tgacgagcga ggctacgacc
     2701 tctgaagcgg tgaactgatg gtcaacaggg ggcagtgtga aggaagctga aaattcgcct
     2761 gccgccatca gacccacgct gggtgtggtg gccctgacgg tctatggcgt gggcaacatg
     2821 ctgggggccg gtatctacgg cctggtgggt aaggcggccg cagaactggg caacctggtc
     2881 tggctcggct tcgtcaccag catggtggcg gccggtctga ccggactctc ctacgcctca
     2941 ctggggtcgc gctacccgcg ggccggcggg gcggcctatg tgacccagcg ggcatttgga
     3001 ctgcccgcca tcagctatgt cgtcggtctg gcggtgatga tgtccggcat cacctcgatg
     3061 gcggcggcca gccaggtctt tgccggttat acccatgaga tcttcaccgc catcccgatc
     3121 aaggcggtgg tgctgctgtt cctgctggcg ctgacggtga tcaactatgt cggcatgcgc
     3181 gagtcggcgt gggtcaatgc gctctgcacg gtggtcgagg ccggcgggct gctgctggtg
     3241 attgtggtcg gttttcgcta ctggggcagt gtcgattacc tcgatgccac gtcgctcaag
     3301 aatcctggcg gcgacttttc ggtacccatg gtgctctcct gcgcggtgct gaccttttat
     3361 gccttcatcg gtttcgagga tctgctgaac atctcggagg aggtgcgcga accgcgccgc
     3421 atctttcaca ttgccctgct ctgtgcattg ggcattgcga ccctgatcta cattgccgtt
     3481 tcgatcacgg cggtttcggt ggtgccccat gcgcagcttg cggtcgaaac cggcccgctg
     3541 gtggcggtgg tgcgggtggc ggcgccatgg gtgccgccgc tgctgttcac cgtcgtggcg
     3601 atgtttgcgg taaccaacac cgcgctgctg aactccatca tggggtcaag gttggcctat
     3661 ggcatggcgc atcaggggtt gctgccgaag ctgctggggc ggctgcatgt ccggcgacag
     3721 acgccgcatg tggcgattct gctgctgatg gcgatcgtgc tcggcttggc cctggttggg
     3781 gacatccgca cgctggccag tgccaccagc ctgttgttgc tcggctcgtt caccttggtg
     3841 aacctgtcgc tgctgcggct gcagcgccgt gccgatgaac ccaagggcgg cttcgaggtg
     3901 ccgcagtggg tgccggccgc gggtgccggc atctccctgc tcttcatcat caaccgggtg
     3961 atcgaagggg tgcgtgcgag tgatctgacg ccggtctaca tcgccggcgg catcatcgtg
     4021 gccatcatcc ttctctatct gctggtgcgg ccacaggcag tggccgacat cgacgggtag
     4081 ggcagcgcag ctctggccgc ccgtcagggt gcctgccgct gctgcccggc gggccgatca
     4141 cttgatgaat gagcagcagt agtcggtcgg ttcgctcacc ctgaccgtga agctcgaagc
     4201 gcccggcacc tcgaaggatt gaccggcagt gatcggccgc cactcgctgc tgccggacag
     4261 ctgcaggtcg agcttgcccg acatgatctc catcagctcc cggtcgccgg tgttgaagcg
     4321 atactctccg ggcagcatga agcccagggt tttcatcgat ccatcggaaa aatgaatgct
     4381 gcggctggtc accttgccgt caaaatagac attggcctgc ttgacgacgg acacgttctc
     4441 gaatttcgac atggctgttc ctgaagaggt tgatcaaagc cggcgattat agccgtgacc
     4501 gggcagccaa tggtgagcgc ggtgcggctg aagaggtcga accaggcggc tgaactgttg
     4561 tagaatcggc cggctttgca tgccgccaac ccatgaaccg acctggcgac gcactgattt
     4621 caggtcatct cgatcattca ctccacagca caggtagtta tccatgccct tgtcgataga
     4681 agaagccaat cagcgcctga tcgccgcttc cggtggcatg ttcgacatcg aagagatcaa
     4741 tatcgatggc gtcatgacca aggtctggaa gtccgcccca cccacgctgc ggcatgtctt
     4801 tctgaacacc aagaactatt tcggccgcga ctattttctg tacgaaaacg agcggatgag
     4861 ctatgagcag cactaccagc gggtggtggc gctggcgtgg cagttgcgca gccgtttcgg
     4921 catcgaaaag ggcgatcggg tggcgctgtc gatgcgcaac tatcccgagt ggccggtggt
     4981 gttctgggcg acggtgacgc tcggtgcggt gatcgtgccg ctgaatgcct ggtggaaggg
     5041 caacgaactg gagtatggtc tggccgattc cggttccaag gtgctgtttg ccgatggcga
     5101 gcgcatcgag cttttgaaag agcacttcac caaactgccc gagctcaagc atgtgattgc
     5161 ggcacgcacg accgcgcccc tgccagccgg catgttgcgc tacgaggagc tgttcgccac
     5221 cgccgccccg gccaacgaac tgcccgaggc cgacattcac cccgatgaca acgccaccat
     5281 cttctacacc tccggcacca ccggcaaccc gaaaggggcg ctgggcagcc accgcaacat
     5341 gtgcagcaac ctgatcagcg gcgctttcgt gcggatgcgc accgaaattc gctatggccg
     5401 cgtgccggca ccaccacccg gaccatcggg tcatctgctg tcggtgccgc tgttccacgc
     5461 caccggctgt cacggcatcc tctgcggcaa cacctttgcc ggcaacatga tcgtcaccct
     5521 gtacaaatgg aaccctgaag aggcgatcag actgatcgag cgcgagaagc tggcctcctt
     5581 cggcggggtc ccctcgatgg tgtggcaggt gatcgagtcg cccgagttcg ccaaacatga
     5641 cctgtcgagc gtgcagagca tcggttacgg cggcgcgccc tcggcacccg agctggtcaa
     5701 gcgcattggc gagagctttc cgcaggtcaa gccgagcaat ggctatggac tgaccgagac
     5761 ttcggcgctc accacttcga actcggccga agactacctc gaaaaaccga ccagtgccgg
     5821 ctgtccggcg ccggtttccg aggtcaggat cgccggcgac gatggccagg aggtgccgcg
     5881 cgggaccatc ggcgaggtgt ggatcaaggg gccgcaggtg gtcaagggct actggcgcaa
     5941 gccggaggcc accgcccaga gcttcaccga tggctggctg cacaccggtg acctcggcta
     6001 catggatggc gatggctttc tctacatcgc cgaccgcgcc aaggacatgc tgatccgtgg
     6061 cggtgaaaac gtctactgtg ttgaggtcga gagcgcgctc tacagccatc cggatgtcat
     6121 ggatgcggcc gtggtcggca ttccgcaccg ggtactgggt gaggaggtcg gcgcgctggt
     6181 gcagatccgg cctggcggca atccgaccga agaggagctg aaaaagcatg tcgccagcca
     6241 gctcgccggt ttcaaggtgc cggtgcggat cgacatgcag cgtgatccgt tgccacgcaa
     6301 tgccaatggc aagatcatga agcccgaagt caagaagctg atgggactgg cctgatccgg
     6361 caagagcgcc ccgcctcttc tggccctgtc gacccagaaa aaggagtctt gtatgagtga
     6421 cttcactcca ggagaggccg ttgccgtcgc ccccaatgtg ctgcggctga ccgcgcccaa
     6481 tccgggcgtc atgaccggtc ccggcaccaa cagctatctg gtcggcacca aggcggtggc
     6541 ggtgatcgac cccggtcctg ccgatgccgg ccatgtcgct gcgctgctgg cggcggcacc
     6601 ggggccgatc cgctggattc tggtgaccca cacccacctc gaccactcgc cggcggcggc
     6661 gctgctgcgt gagaagagtg gcgcccagtt ggtcggaatg ccggctccgg agcatggggc
     6721 acaggacaaa aagttcaaac cgggtcgcat catgatccag gatgaacggc tggttggtga
     6781 cgacttcgtg ctgaccgcca tccacactcc cggtcatgca tcgaaccacc tctgctatct
     6841 gctggaggag gagggcctgc tgttcaccgg tgaccacatc atgagtggtt caacggtggt
     6901 gatcgccccg cccgatggtg acatggccag ctatctggcc tcgctggcac tgctgaagcg
     6961 ctatccgctc aagcggttgg cgccggcgca tggcggcatt ctggagcagc ccgaagcggt
     7021 gatcgactgg ctgattgccc accggctggg gcgcgaggcc aaggtggtcg aagcattggc
     7081 ggcggcgccc gactccaccc tcgatgagct gctgccggtg gtctatcagg atgtgcaggc
     7141 cctgctgttc ccggtggcaa agcactccct gacggcgcat ctgctcaaac tgaaggatga
     7201 agggcgtgcc cgtcgcagtg acaacggccg ctggcgcaac tgctgatcag gcgatgcagc
     7261 cgaggcctgc catgcacgaa taggcgatgg cgagacccag cgtgatcaga tagccggccg
     7321 ccagttgcag ccccaccagc acgatgcgca gcggcagcgg ctggccagcc agcccccgat
     7381 accaggcgat ggctgcggct gcggcaatca aggccaatcc cagcttcatg gtgtcgtcgc
     7441 ccaggccggt gaaatggccg ttgctgcgca gccaatggtc gatgccgagc agggcgatac
     7501 agcccagcag cgccccgatc caaccaccca ccagatgctt catcactcat gctctcctga
     7561 tcgctgcggc attgtagccg acaacggcgc tgtcggctgt cgcgctttgg cagtgcaacg
     7621 ccccgcactc atctgcagat tcttttttat ggtctaggct tgatgtcatg ggatcttccg
     7681 tcgcagtgcc agacaaagat cgctcgactg tgtgagcaga gaattcaagc actaccccgg
     7741 tcaggggtgc cacattgaat ggagaagaag agatgagcag acttttgaca ccaggcatca
     7801 acctgatgaa aaagatgaac ttcctgtaca agttcggttt gatttcactg gccgtgctgg
     7861 tgccgctgat gctgctgagc gcactgctgg tgatgcagac cagccgtgac atcggcacga
     7921 tcagagccga gcgtgtcggc atcgaacatg cgaaggtcgc gctcgagctg cgccgactca
     7981 gtggccgcta tgtcgatctg gtgctggcga tcgaacaggt caacaagcag atgagtgccg
     8041 ccaacgacct gacccccctc tatcgccaga cccgccagca gatcgacgag gcgatcaagg
     8101 cctatcaggc cgtcctggaa caacagccgc tgggggagga gacccagagc agttggacca
     8161 acctgctgaa cttctggcag cagagtgcgc agatcgaccc caaggcgggc gtcgaagtac
     8221 cacaacatct ggcccatgcc gagcagaaaa agctgctgga gctgatgcag cacttcattc
     8281 tggcggtgtc gatcgaaagt ggcctgatcc tcgacagcag cccggaacgc tacctgctga
     8341 tctcgcatgt gctgaatgag ttcacgatgc tggcgggccg cctcgaagag gcgcgggcgg
     8401 tgggcacctc gatcaaggcg cagacggcga ccacggccag cacgctgaag tggctcgaac
     8461 cgatcggtca gaaactgctc gacaccgaag aggaatacca ctatgccgtc gccaccagca
     8521 tcggccgttc tccgcgcgcc aaggccctgc tggaggctga tgccaacaag gctggagagg
     8581 cctacaaggg gatggccgaa tacatcgagt cggtgttgat gatgggcgaa gagcagagcc
     8641 tgacctggga gggctactac aatcacctga gcgcgaagat cgatccgatc ttcaaggttg
     8701 gccagtcgac gctgccgctg gtcgacacca tgttggttga gcaggagagc gcgctcatcg
     8761 cgcagcgaaa tggttatctg attaccgcct tggttgttct ggcactggtg gtctatctct
     8821 gtgccgcctt tgccagttcg atgaaggtca ccatttccag catctatgct gccgcacgtg
     8881 ggctggcgca gggcgacacc cgggtgcggg tggcggtgga cagcaaggat gaactgggca
     8941 gcctctctgt cgagttcaac agcatggccg agaaggtggc ggaactgatc agccaggcac
     9001 accacaccgc acggcaggtg tcggagcagg ccgaaaccgt ggagcgcatc gccagccgca
     9061 gcagcgatga gttgcagagc cagttgagtg agaccgcgca ggtggcgacg gcgatgaacg
     9121 agatggccgc cacggtgcag gaggtggcgc gcagttcggt cagcgcagcc gattcggcac
     9181 aggcggccca ccgtgacgtg cagagcggcc ggcaactggt cgagtcgacc tcggcgacca
     9241 tccgccgtct ggccgaggag attggtggca ccgtgcaggt gacgcagcgg ctggtggagc
     9301 acagccacag catcagcaag gtggtcgatg tgatcaaggg cgtggccgag cagaccaacc
     9361 tgctggcgct caatgccgcc atcgaggcgg cccgggccgg tgagcaggga cgtggctttg
     9421 ccgtggtggc ggatgaggtg cggaccctgg cgcagcgcac ccagcagtcg accagcgaga
     9481 tcgagcagat gatcggtcag ttgcagaccg gcgtgaacga tgcggtcaag accatgggca
     9541 gcagccggga gatgaccgaa cgcaccgtgg tggagtcatc gaaggtggat gaagcgctgg
     9601 ccagcatcgc cggttcggtc gacaacatcc tgggcatcag ccagcagatc gccacggcag
     9661 ccgaggagca gagcgtggtg gcacgcgaga tcgaccgcaa catcgtcgcg atcaacggtg
     9721 ccagcgaaca caccgccagc ggtgcccgcg agacggtgga agccagccag aacatggcgg
     9781 cgctgaccca gcaactggag cagttgatcg gcaccttcaa ggtgtgagag caaagcaggg
     9841 cgaccacccg acagggggtc gcccaaacga aaaacccgcc ttgaccggcg ggtttttccg
     9901 tttcagcggc cgctgccgat ggcgtggggt tgatggctga tcacctcctc gtaatgccgg
     9961 ttgatctcct gggtcagcgc ggcgatgcgg cgctgccagg cgccctcgaa gtcggcattc
    10021 catgcagtgg cgacggcatc gcgcaccgtc tgccgaatgg cgtcgaacag cgcgctgtac
    10081 atcggcagaa gcacgccgtt gttggtgtgg gtcagcacct cgaagccgag tacggtctcg
    10141 agttcgtcgc catcgggcat catgatcagc atcatcgcct cgtccagcat ccgtgcccgc
    10201 atcaggtggt ccatgtgctc catcatatgc gccgattcgg ggctggccgc gaagtagcgt
    10261 tcatagaccg ctgcggagat gtcaccggct ttgtcggcag tctgctcgaa ggaggcgagg
    10321 atcaggtcgt tgtcgttcat ggcgggctcc gggagtcgtg atggtgggca ggccggtacg
    10381 agcggccaag tcattctacc gtagaaaata tggtttatgc cgctcctgtc cggtcggcgg
    10441 attgcggagt ctggcgtcag ttcggtcgat aggtcccctt gaaggtggtg ccgacctgca
    10501 ggatgcgcag cgtgttggtg gcgcccggtt tgccgcggtg gtcgccctcg gtgatgatca
    10561 ccagatcacc cttctgcacc gcgccctgct gttcgagcag gtcgatcgca cccttgttga
    10621 cctcgtccgg ctccacctgg gtgtagtcgt acagcaaccc ctcaacatcg cggtagagcg
    10681 ccacccggcg cagggtcgag tggtggcgtg acagggcgta gatcggctgc gccgagctga
    10741 tgcgcgacat ccacagcggc gtggcgcccg attcggtcag gcagaccact gccttgatgc
    10801 cggtcagatg gttggcgatg tacatcgacg ccatcgcgat cgcctcgtcg atgcggtgaa
    10861 actcggagtc gacccggtag gaggacgaac gggccgaagg ctgcttctcg gcgccgatgc
    10921 aggtgcgcgc catcgcccgc accgcctcga ccggatggtg gccggtggcg gtctcagccg
    10981 agagcatcac cgcgtcggtg ccatcgagca ccgcgttggc gacatcgaac acctcggcgc
    11041 gggtcggcac cggattgtcg atcatcgact ccatcatctg ggtggcggtg atcaccaccc
    11101 ggttcagggt ccaggcgcgc ttgatgatct gcttctggat gccgaccagc tcccagtcgc
    11161 ccacctcgac ccccagatcg ccgcgtgcca ccatgatgac gtcggcggcg gtgatgatgc
    11221 ggtcgagcgc ggcatcgctg gcgacggcct cggcccgttc gatcttggcc accaccgcgg
    11281 cctggctgcc ggcctcacgg atcagttgcc gggcggtgtc gatgtcctcg gccgagcgca
    11341 cgaaagagac ggcgacaaag tcgacgccga tggcggcggc ggtgacgatg tcggcctcat
    11401 ccttgtcgga gagcgccggc gccgagagcc cgccaccctg gcggttgatg cccttgtggt
    11461 tgctgagggg gccacccacc agcacctcgg tgtcgatgcg gtgggtggtg atgctgtcga
    11521 cccgcagctc gatgcggcca tcgtccagca gcaggatgtc acccggccgg caatcggccg
    11581 gcagcgccgg atagtcgatg cccacctggt ggatgtcgcc ctcgaactca tcgagcgtgg
    11641 catcgagtgc gaagggcgca ccgcgctgca aggtgacctt gccatcgcgg aacgagcgca
    11701 cccggatctt cggcccctgc aggtcggcca ggatcgccac attgagattg tgccgggcgc
    11761 tgatttcgcg caccatgcgg gcgcgcgcct gatgctcctc ggcctcgccg tgcgagaagt
    11821 tgagccgtac gacattgacg ccggccagca gcatctgctc cagcacctgc ggtgcatcgg
    11881 tggcggggcc gagggtggca acgatcttgg ttcgacgcaa catggtgcct ccgaggatgg
    11941 cggtgtgggg tgctcttcgt cgcagcagaa catttcgtgc gtgtcgctgt ccattgccaa
    12001 catgcgggcg gccagtgcgg tgctttctgc acccgttcca ataccagcgg actccaatgc
    12061 ctgttttttc atccctgcgt ccggtcctga ccatgctgct gtgcgggctg ctgttcgtca
    12121 atgcagcgct gcgtgcagag ccccaggtgc cgatcgggca ggatgagccg cagttgccct
    12181 atctgggtga tgtcagctcc agcatcatct cgaccgacga ggagcacttt ctcgggcaga
    12241 tctggctgat gcagctgcgc agccaggtca ccatgctgga tgatccggtg gtcaaccact
    12301 atctggagaa cctgctcggc cagttggccg gcagcagcca gctgcaggac cgccgcctga
    12361 cactggtggt gatcgacagc ccggagctga acgcctttgc cgtgccgggt ggtgtggtcg
    12421 gcgtcaatgc cgggctgttc agccatgcgc ggaacgaggc acagctcgcc tcggtgctgg
    12481 cccacgaact ggcgcacctg agtcagcggc accatgtgcg gatggtcgac agcgcacgca
    12541 agaacagcgt gccgatgctg gcggcgatgc tggccggcgt ggtgctggcg gcctcgggcg
    12601 gtggcgatgc cggcatggcg atgatcaccg gcagtcaggc ggcgctgatt caggagcagt
    12661 tgcgcttcag ccgccagtat gagagcgagg ccgaccgcat cggcatgctc aacctggtcg
    12721 cggccggcta cgatcccaag cagatgccgg cgatgttcga gcagatgttg caggcccgct
    12781 ccttcgccgg tgaccgggcc ccacaatttc tgctgaccca cccgttgacc gaggcgcgca
    12841 tcgccgacac ccagaaccgc gtcgcgcagt atcccgacaa tgctgaggcc aaggtcgaca
    12901 gcatcgaata cgagcaggcc cgcagccgca tgcagaacgc cctcgagggc aatccggtgc
    12961 gcatcatcaa gcaggtcgag gccacgctgg cgacgcaacc gggcggtgcg ctggacgatg
    13021 gcaaccgcta tctgctggcg ctggcgcaac tgaaggcgca gcggccgcag gaggccgaag
    13081 ccaccctcaa accgctgctg gagcgtgagc cgctggcgct gggttatctc tatacccagg
    13141 tgcagatcga catggcccgc ggtcgcgcgc aggcggcgct ggcacggctc gatccggcgc
    13201 tgaagcgtgc gcccgacagc tatccgctga cgatcgccca ggctgaagcg ctgctggcca
    13261 gcggtcgacc ggcggcagcc gagccgctgc tggcccggct gagccgggaa cggccgtggg
    13321 atgccccggt ctggtatcgg ctggccgagg cgcggggacg ctcgggtgat gcgcgcggca
    13381 tgcaccgggc gcagggcgag tatgcgctgc tgatggggca gatcgacgat gcgcagcggc
    13441 agctcgaata tgcccgcgcg ctgtcgcagg gcaactacgg cgagagtgcc tcggtcgacg
    13501 agcggctggg gcagattcgg cggctgcgca agcagttggg catggcgacg cgctgaatca
    13561 gcgtggcggc aggcttttcg agaaggccag catccgctcc agcggcacca tggcctgccg
    13621 acacagctct gcgtcgagtt cgatctcgtt gcactcatca cgcaggctga ccagcagatt
    13681 ctccagatgg ttcatcgcca tccacgggca gtgggcacaa ctgtgacagg tggcgccacg
    13741 gccgccggtg ggggcctcca gaaaacgttt gcccggcgcg agctgttcga gcttgtagat
    13801 caggccgcgg tcggtggcga cgatgaaggt gtcgttgggc agcctgcggg ccgcctcgat
    13861 gatctgggtg gttgaaccga ccagatcggc cagatcgagc accgtcttcg gcgattcggg
    13921 gtgggccagc acagctgcgt cagggtagac atgcttgagg tcgcgaatgc cttgggcctt
    13981 gaactcctca tggacgatgc aggcgccgtc ccacagcagc atgtcggcgc cgctctgctc
    14041 ggccaggtag ctgcccaggt gcttgtccgg tgcccagaga atcttctgac cctctgcgct
    14101 cagatgttcg atcagttcga gcgccatgct ggaggtggcc acccagtcgg cacgcgcctt
    14161 cacggcggcg gaggtgttgg catagaccac cacggtgtgg tcgggatggg catcgcagaa
    14221 ggccgaaaaa gggtcgatcg ggcagctgat gtcgagtgaa caggtggcct ccagcgttgg
    14281 catcagcacc cgcttgtgcg gtgtgagaat cttggcggtc tcgcccatga agcgcacgcc
    14341 gaccaccatc agcgtggtgg cggggtgctc cttgccgaag cgggccatct gcagcgagtc
    14401 ggagacgaag ccgccggtct cctcggccag ccgctgcaac tgcgggtcgg tgtagtagtg
    14461 ggcgacgatg accgcattct tgctggccag cagcgcctcg atctgccgtt tcagtgcctg
    14521 atactgcgct tcggccaagt gatgcccgtc gcgggcttcg acgaagaggg tttcgtcgat
    14581 ctggtgactt ttcagcagct tttcggtcac gttcatgggc agtgcgacct ctcggcgatc
    14641 cgggtgtgat gctgggcccg attgtagcat ggcccacccg gcgcgcatgg ccgggccgtc
    14701 agcgccaccg tgcagctcag tcgtcgagcc actgccggat gctggtcgcc tggtgggggg
    14761 cattgccgat gtaaccggcc ggggtgagct gccgcagccg ggccttttcg gcctccggaa
    14821 tcgccaactg gtcgatgaac tgttgcagcg ctgcctggtc gatggcctga ccgcgggtca
    14881 attccttcag cttctcgtag ggctcctcga cgccatggcg gcgcatcacc atctggatgg
    14941 cttcggccag cacctcccag gcactgtcca gatcggcggc gatccgttcg cggttgatct
    15001 ccagcttgcc gagtcccttg agtgtcgagt gcagggcgat agtggtgtgc gcaaaggcgg
    15061 tgccgaggtt gcgcagcacg gtggagtcgg tcaggtcgcg ctgccagcgc gagatcggca
    15121 gcttgctgga gaagtggtgc agcagcgcat tggcgatgcc gagattgccc tcggcattct
    15181 cgaaatcgat cggattgacc ttgtgcggca tcgtcgagga gcccacctcg ccggcgaccc
    15241 gcttctgacc aaaatagccg agcgagatgt agccccagag gtcgcgggtg aagtcgatca
    15301 gcacggtgtt gaagcgggcg atggcatcga acagctcggc caggcagtcg tgtggctcga
    15361 tctgggtggt gtaggggttc cacgccagcc ccaggtcggt gacgaagcgg ctggccagcg
    15421 tcggccagtc gagctcgggg tagctgacgc agtgcgcatt gaagttgccg acggcgccat
    15481 tcatcttgcc gagcagcgca gtggtggcga tctgctgccg ctgccgccgc agacgatagg
    15541 cgacattggc gaactccttg ccgacggtgg tgggcgatgc gctctggcca tgggtgcgcg
    15601 acagcatcgg caagccggca agctgcacgc tgaactcggc gatggtgtcg atcagtcgat
    15661 cgatcagcgg cagcaggatg tcgttgcggg caccctgcac catcagcgca tggctcaggt
    15721 tgttgatgtc ctcggaggtg cagccgaagt ggatgaactc cagccgtggc cgcagttccg
    15781 gctggctggc gaagcactgc ttcaggtggt actccaccgc cttgacgtca tggttggtgc
    15841 cgcgttcgat ctgcttgatc tgctcggcgg ccgcctcatc gaactggtcg accagggagt
    15901 tcagaaaatg gttggccgcg ctcgacagcg gcggcagctc gggcagttga ccggattgcg
    15961 ccagatgctg aaaccagcgc acctcgacca gcagccgatg gcgaatcagg ccgaactcgc
    16021 tgaagtagcg ccgcagttcg gtgcttttgt cggcatagcg gccatcgatc ggggagagtg
    16081 cggtcagggt cgagagtgtc atgggcggtc cggggttgac ttgaaggggg cagtttcgct
    16141 gtgcggggtg gtcagtggcg ctggagcagg cggtcggccg tgttgatcag ggcgcgccgc
    16201 tgccagagca aaaacagcgg gcgtccgccc agctggcgcc agagcatggc cgagcgcacg
    16261 gcggcgaaca gcagcaggcg aatcttgtcg gcgatctcgc tgttctgcag atggcgcggc
    16321 tcgccgacca cctggatgcg aaagcgcagc gtgctgaggc tctgctgata gagccgcgag
    16381 agctgaccga ccagtgcggc ctggtcgaag tcgggcaact gctgctggcg gccgatctgg
    16441 accagctcct ggccgatctg atcgagcatc cgtggctgcg ccgcgagctt gccctgcagg
    16501 tggatggcag ccaccgcata gcgcagcgta ctgaccatct cgtcactctt gccgttctgg
    16561 cgccaaccac tgagcaggcg cagcccgggc aggatcggcg agaggccacc gaacaccgcc
    16621 tcggtcgagg cgggatgggt gcagagggtc gccgtcacgg cagtctcgat gttcgattgc
    16681 gagaccacgc cggtgcgggc gaggcgatcg accccctggg caaactgcag gagggcggca
    16741 aaggccaggg cctgttcctc gatcgatctg gccatctagg ctccgtggcg gagggcaggg
    16801 gaaaacggct gggccggctg catcacaggc agggcggttt cgatcactcc accgcccagg
    16861 cagatctcgt cctgatagaa caccacatac tgccccggcg tcaccgcccg ctgcggcgtg
    16921 tcgaaccgga cccgacagcg gccgtcctcg gccggctcca gccagcaggg ctgatccggc
    16981 tgacgatagc gcgtcttggc ggcgagccgg cagggcaggc gtgggccctc accatcgatc
    17041 cagtggagcg ccgaggtggt gagtccatct gccagcagca gcggatggtc atggccctgc
    17101 accacgatca gcgtgttgcg gtccagatcc ttggcggcca cataccaggg ggcctcggcg
    17161 gcgccatgga caccgccgac acccagaccc tggcgctgcc cgatcgtgtg gtggagcagc
    17221 ccgtggtgct cgccgagccg gcgaccctcg gcggtttcga tcggccccgg ctgcgcgggc
    17281 agatagcggg tcagaaaatc gcggaagcgg cgctcaccga tgaagcagat gccggtgctg
    17341 tcctttttgc tctgggtggc gaggccgagc tgagcggcaa tctgccgcac ctgcgacttc
    17401 tgcagcgctc cgaccggaaa gtggctgcgg gcaaactgct gtgcgctgac cgcatgcagg
    17461 aagtagctct ggtccttgtt ggcatcgagc cccttcagca gccgggtctg cccgccctgc
    17521 cgatcgaggc gggcatagtg gccggtggcg atctggtcgg cacccagcag ttcggcatac
    17581 tcggcgaaga ccttgaactt gatctcgcgg ttgcacagca catcggggtt gggggtgcgg
    17641 ccggcgcggt attcgctcag aaagtggctg aagacctggt cccagtattc ggcggcgaag
    17701 ttggcggtgt gcagttcgat gccgagctga tcggccacct gccgggcatc ggccaggtcc
    17761 tgtttcgccg tgcagtattc ggtgccgtca tcctcctccc agttcttcat gaacagtccg
    17821 accacctggt ggccctgctg cttcagcagg aaggcagcga ccgaggagtc gaccccgccg
    17881 gacatgccga cgacgatgcg ttgtgcgaag gagggtggca cctgctgtga ccgggcgagc
    17941 aaaagggcgg caattatagc ctattgcccg gaatgctcat gaatgaaatc gagcggatag
    18001 cgcttgccgc gcaggtagtc ctcgacgttg cgcagcacca gcgggctgcg cagccggtca
    18061 cggcgggcgc gcagttcgtc gacggtcagc cacagtgtgc gcaggatgcc ggtgtcgagc
    18121 ggacgttcgg gatggtgccg cagcggttcg gcaatgaagc aggtgcgcag atagctgatg
    18181 ccgttgtagg ccgaggtgta ggtatagagg ccgagcagcg ccttgacggt gacctcccag
    18241 ccggtctcct ccagcgtctc gcgcacggcc gcggcggcca gggtctcgcc ctgatcgagg
    18301 tggccggccg gctggttgat gaccaggcgc tcgccctcgc tctcttcgac cagcagaaag
    18361 tggccatcgc gctccacgat ggtggcgacc gtggcgtggg gataccaatc catgcagggc
    18421 tccggtggat gactggcaat tgggataatg gacgctttat agaataatac agccagacgg
    18481 cttcccgccg ccgaccctga tcgaccggac agatcacatg agccagctca cccacctcga
    18541 cgccgacggc aatgcccgga tggtcgacat caccgacaag gccgagagca gccgggaggc
    18601 cactgccgag gcgatcgtgc gcatggcgcc cgacacgttg cggctgatca ctgagggacg
    18661 gcacaagaag ggcgatgtac tcaccgtcgc ccgcattgcc ggcatccagg ccgccaaacg
    18721 ctgtgccgag ctgattccgc tctgtcatcc gctgctgctc agcgccattc aggtcgaact
    18781 gacgcccgac atcgcgcaga gtcgggtggt gatccgcgcc acctgccgcc tgagggggca
    18841 gaccggtgtc gagatggagg cgctgactgc ggccagcatc gccgcgctga ccatttacga
    18901 catgtgcaag ggggtcgacc gggggatggt gatcgatggc gtgcgcctgc tcgagaaacg
    18961 gggtggccga agcggccatt ggcaggccga gacgccgtcg acgccatgaa gatctgctac
    19021 ttcgcccgcg tgcgcgaggc cctggggctc gaccatgagc tgctgcaacc gccgccgcag
    19081 gtcgatcggg tcgatcggct gatggcccat ctgatcgccg cacgcggccc gctgtgggcc
    19141 gaagtgttgg accccgagcg catcctcgtt gcagtcaacc agcagatcgc cgccttcgac
    19201 acgccgattg gggcggacga cgaagtggcg ttctttccgc cgatgaccgg gggttgagat
    19261 gttcgaggtg cgggttcagc agcacgattt ttcgctcgaa gccgaggtga accagttgcg
    19321 ccagcaggcc ccgggcgccg gggcggtggt cgccttcgtc ggcctggtgc gggagctggg
    19381 tgaggcgcgg cagattgagc agctcgttct cgaacactat ccgcagatga ccgaaaacag
    19441 cctgcggcag atcgtcgagg aggcgcggca gcgctggccg ctgctcgccg cgcgggtggt
    19501 gcatcgggtg ggggcgctga caccggccga gcagatcgtg ctggtgacca cggccacggt
    19561 gcatcgccac gacgccttcg cggcggccga attcatcatg gattttctca aaacctccgc
    19621 acccttctgg aagaaagagc agggcgtcag cggcgcgcac tggatcgacg cgcgcgacag
    19681 cgaccagagg gcccgcgaac gctggcagcg ctgagtcatc ctctgggcga cctctttggt
    19741 gcgcgttgcg gtcttgatgc cggcactcgg cgggggtggg gtgccgtctg cgctggcgac
    19801 tcgatctgaa cgctgcgcca ctcaccgggc gccagaccat cgagtgtcca gtcgccgatg
    19861 cgccagcgca ccagccgcag tgtcggcaga ccgactgcgg cggtcatgcg ccgcacctgc
    19921 cggttgcgcc cctcgcggat cgtgatctcg agccagtggg tgggcttgtt ggcgcgccag
    19981 cgaatcggcg gctgccgtgg ccagagcgcg ggttcgtcga tccgccgcac ctgtgccggc
    20041 aacgtcatgc catcgttcag cagtaccccc tgttgcagcg tgccgatcgc ctcggtggtc
    20101 ggttctcctt cgacctgcac ccagtagctc ttctgctgct tgtggcgcgg gtgggcgatg
    20161 tgatgctgca actggccgtc gtcggtcagc agcaacagcc cctcggagtc acggtcgagc
    20221 cggccggccg gatagacgtt cggcagctcg atgaacggtt tgagcgtggg atgggcgcca
    20281 tcgcccgaaa actgcgacag cacgccatag ggtttgttga acagaatcag cagcgccatg
    20341 cgcggtcatc aaaaggtaaa aaaattaccg gaacggccaa aacggcgatt tcgctttggt
    20401 atactccggc catcttcggc gacgggttga aatcgaatta ccgaaccgca actgtcgttc
    20461 tgccctgcat ggatggtgcg ggtcgcgggt gaggcagtct acccatcagt cgctcccctt
    20521 ggaatgcatc tggagcagtc aggaccatga aacggacaaa gctggtgatt cccaaagacg
    20581 gtgaaaagat caccgccaat gcggatctct ctctcaacgt gcccaaccac cccatcatca
    20641 cctacatcga gggtgacggc accggtgtcg acatcacgcc ggtgatgctg aaggtggtca
    20701 acgccgccgt cgacaaggcc tatggtgcgc gccgttcgat tgcctggatg gagatctacg
    20761 ccggtgagaa ggcgacccgg gtctatgggc cgaacgagtg gctgcccgcc gagaccctcg
    20821 aggctttcag ggagtatctg gtcggcatca aggggccgtt gacgacgccg gtcggcggcg
    20881 gcattcgctc gctcaacgtg gcgctgcgtc aggagctcga cctctatgtc tgcatccgcc
    20941 cggtgcgctg gttcaagggg gtgccgagcc cggtgatcgc accgcagaag accgacatgg
    21001 tgatcttccg cgagaactcc gaggacatct atgccggcat cgaatggaag gccgattcgc
    21061 ccgaggcgat caagctgatc agattcctgc gcgaagagat gggtgtcacc cagatccgct
    21121 tttcggagca ttgcggcatc ggcatcaagc cggtctccga agagggcacc aagcggctgg
    21181 tgcgcaaggc gatccagtat gcgatcgact gcgatctgcc gtcggtcacg ctggtgcaca
    21241 agggcaacat catgaagtac accgaagggg ccttcaagga ttggggctac gagctggcgc
    21301 gggagcggtt cggcgccacg ccgctcgatg gcggaagctg gcacgccttc aacaatccca
    21361 gaaccggacg gcagatcgtc gtcaaggatg tgattgccga tgcctttttg cagcagattc
    21421 tgatgcggcc cgaagagtac agcgtcatcg ccaccctcaa cctcaatggc gactacatct
    21481 ccgacgcgct ggcggccgag gtgggcggca tcggcatcgc ccccggcgcc aacctgggcg
    21541 gcagcgtctc ggtgttcgag gccacccacg gcacggcacc caagtacgct ggccaggaca
    21601 aggtcaatcc ggggtcactg gtgctctcgg ccgagatgat gctgcgccat atcggctgga
    21661 gcgaagcggc cgacctgatt ctgaagggcg tcgagggcgc catcgaggcc aagaccgtca
    21721 cctacgactt cgagcggctg atgcccgatg ccaagctgct gaggtgctcc gaattcggcg
    21781 acgccatcat caggaacatg gaaggctgat cgagcgctgc gcctcgggtg gccatgccga
    21841 tcgctccact gcccggcatg gcgccaccgc ccgcccatca tggcgtcatg ccaccgatca
    21901 ccgccagaaa cgcctccccg taacgctcca gcttggcggc gccgacgccg ttgatctgcg
    21961 ccagatccgc cagcgtccga ggccgctcgg cggccatctg cgccagcgtc gcgtcgtgaa
    22021 agatcacgta ggccggcacg ccgtgggcgt ccgccagcct tcgccgctcg gcgcgcagcg
    22081 cctcgaacag cgcctgctcg gcgctcgcca gcagcggcgc cgcactgccc ttgcgccggc
    22141 gccgctcgcg ggcggtgttc tcctcacgaa gaaacagccg ttgctcgccg cgcagcaccg
    22201 gccgcgccgc ctcggtcaga cgcaggccac cgtggccgtc gatctccaca ctcaccagac
    22261 cccgcgcggc aagctggcgg aacagcgagc gccactgcgc cgctgggcgc tcctgcccgc
    22321 ggccaaacag agcgagccgg tcgtggccga agcttgcgat gcgcggatca tccccgccgc
    22381 gcagcacctc gaccagatag gcgacgccaa agcgctgccc ggtgcggtag acgcaggaga
    22441 gcgcgagctg cgccgcctcg gtggcgtcga aggtggcggg cgggtcgagg cagttgtcgc
    22501 agttgccgca gggcgccggg agttgctcgc cgaagtaggc cagcagcgcc tggcgccggc
    22561 agccggtgct ctcggcgagg cccagcagtg cgctgaactt ggcctgctcg acgcggcggc
    22621 gctcctcggg cgcatcgctg gccgccagca actggcgcgt gccgaccacg tccgccaggc
    22681 tgtaaccgag ccaggcgttg gccggcaggc cgtcgcgccc ggcgcggccg gtctcctggt
    22741 aatagccctc gacgttcttc ggcagcccca gatgcgccac gaagcgcacg tccggcttgt
    22801 cgatgcccat gccgaaggcc accgtcgcca ccatcaccag cgcatcctcg cgcaggaagc
    22861 ggcgctgatt cgccgtgcgc gtctcggctg ggagtcccgc gtggtagatc agcgccgggc
    22921 ggccgtggcg ctccagccag tccgcggtct cctccacccg gcggcgggtc gggcagtaga
    22981 cgatgccgct gtcggtgcgg tggtgtgcat ccagaaacgc cagcagctgc tcgcgtaggc
    23041 cctcgccctg gcggatgtgg taacggatgt tcgggcgatc gaaactcgcc gtgaacaccg
    23101 ccgcagcggt gagcccaagg cgctgcacga tgtcgcgctg cgtcggcccg tcggcggtgg
    23161 cggtcagcgc cagccgcggc acgccgggaa agcgctcgcc gagcacacca aggccgagat
    23221 attccggccg gaagtcgtgc ccccactgcg acacgcagtg cgcctcgtcg atcgcgatca
    23281 gcgccaccgg cacgcgcgac agcagttcca gcgtgcgctc gccgaccagg cgctccggcg
    23341 ccatgtaaac caccttgagc cgcccggcga gcagatcgcg ctcgatcgcc gccgcctcgc
    23401 cggccggcag gcccgagtgc agcgccgccg cggccacgcc gagctcgcgc agcgccgcca
    23461 cctgatcgtc catcagcgcg atcagcggcg acaccaccac gcccacgcca tcgcgcacca
    23521 gcgccggcac ctgatagcac agcgacttgc caccgcccgt gggcatgatc accagcgcgt
    23581 cgccgccgcc gaccacgtga tcgatgatgg ccgcctgccg gccgcgaaag gcgtggtagc
    23641 cgaagacgcg ggtgaggatg tcgagcgggg tgtcgaggcc ggcgagtgtc atggggcttc
    23701 gtctacctcg gtgacttcgc tgcagcgcgt gcgcccagca gacgaccggg cccgtgggcg
    23761 acgaggtcat cgcgcgacgc caaaccgagc ccacagtcac tgtgcccgaa acacatggcc
    23821 tcggtgccag gccgcagggc atgcgggctg tgccgcgacc gcgtgagatc gcgacccggc
    23881 atgatgaacg ccagccagcc gtcggtcgct cccggttcca ccacgttgcc ggcgccgtgg
    23941 gcgagcttgg cgagcgggat tggcgagagg ccgttcacgt cgtcaacgcc agccggttgg
    24001 aggcgggcat gcggcggcgc cagtgggtgt cgatcgcctg gacgaaatcg gcgcgcacca
    24061 gcttggcccg cgcttcgcac cagtcgccgg cgtcgcggcg cagcacgaag ccttcgacgt
    24121 gcggcgcacc gcaggccgat ggcgtttctt cgagcagctg cttcaccatc gccagcgtca
    24181 gccggccgcg agtgacttcg ggcaccgccg gtatgtcgat ccgcgccagc agcgcgttgc
    24241 gccgcgccag gctccagaag cgttggctgg cccggtcata gatgtcgaag ccgacgaacc
    24301 agtccggcaa cgcggtgtag gcaatgctgt gggtcaaggc gcaccattcg ccgaacagga
    24361 tcagctccgg cgtcagcgcc tcgcgcagcc cggcctcatg ctgcgccagc caggccggca
    24421 gccgcgcgaa ctggccggca tggggttcgt acagatactg gccgcggttc tgggcgcgca
    24481 gtctgccatc cggcgccagc gatagcccca ggtttgcgcc gtcgagcttc tcctccacca
    24541 ccacttcacc cgccagcagc gcctgtgcct cggccggcgc gagcaccttg tcgtcgcgcg
    24601 gcacgcccgg gccgagccag gccaggtgcg gggtgtgggg aaagcggaag aaatcggtca
    24661 tgtcgcccgt tcaacgatgc cgtttttcgg catgtttctg ccgtgaccaa gcctcgatga
    24721 ttggctccag ttgcacctga tgcgcggcca gtgcgcgttc ccaatcgtgc caatcggtat
    24781 cgccggcaaa ggcaatgcaa ccctccccaa acgcttcacg cgcagccaac gccgccgcta
    24841 ccatttttcg gtctgcaaga tcatggacaa cagaagcaag cgttgccggc aagacaccat
    24901 gaccatcgtt atcgtagaca acctctaccc cgtccaccgc cgccgtgctc catttgtgca
    24961 tgacgacctg aatgccgtag gcattgaagt caagatttcg attgttgatg tattcgtcgt
    25021 aaatgcgacc ggccaaatcc agcaccatcc gcgacgggcc ttgctcgaag gcgtacagcc
    25081 agtcccagat caccttgcgc aaaccggggt ctgccggggt agcgtcaata tccttcgggt
    25141 gaatcgggtc cgccgcgctg gcggcgatca gcacattagt gtccaccacg taacgcgcct
    25201 tcacggcgcg ctctttttct ggcgctcaaa catcaggcgc gcctgctcgc gcgtctcgcc
    25261 cagtgcgtcg ccgaagaatc cgggcggcca gtcgggaagg cgaccaaagt cgtccagcgc
    25321 cagcgtccga aggtgagctg acttgccctt gcgctcgacg aaatagagcg ccacatcgtc
    25381 tgtggacact ttctgcctgg ccaccagggt ctgcatgcgc cggaacagat gttctgaatg
    25441 ggtctccacg atgaactgca ccccgcgctg tcgactgacc tcgacgaaca gctcggcaag
    25501 tacggattgc gccagcggat gcaggtgaat ctcgggttcc tccaggatga tcgtgctgcc
    25561 acggggcgcg aagtaggcca ccaccaatac cggcagcacc tgcgacacgc caatacccac
    25621 gtcgcgcagg ttgcaggcca cgccatcacg gtggatcacc aactcatagc ggttcgatcg
    25681 cccctgctgg cgcacctcca gattttcggc caccttcatg cgcttgagcc atttggagac
    25741 accctctacc acgacgttct gttcctcgcc tttcagcaac gcactggcca gcagtgcatc
    25801 gatggcggca cggccgtcgc tgcccacgtc gccgggcttg gtcttgttcc acggataatc
    25861 gcgttccggc ttgcggcgca gtggccccag atagctgatg ctctccaact cgcggcggat
    25921 ggcgagactc aagtcctcgg ccagtggccc atccttgtcc agccttgcaa tgacatcggc
    25981 agagagcgcg atcgaacgct ccggcgcgca atcccggccc ttcatgcgcg gctgcgattc
    26041 ttcgtccacc aggatagaaa acgcgccttt gtcacggcgc acggcctgaa agcgtcgtga
    26101 atctgtggac agcttcaacg tttgtacggc cacactgcgg gatgaggtct ggccgtagct
    26161 gcaatcgaat ccgccttgtt tgatgcgctc gtgatcatcg cgcatgaaat cgaaggcaat
    26221 cgagaattgt cgcggcgatg cggccgcctg gttcaacaca tcgtcgaagc tgccgaaatt
    26281 gaacaggtcg ttgacctcat ccccgcccag attcaggtgc accgtgcggt cgggggactg
    26341 cacggtctgc ttgagcagca gcaggctctg gatcagggag gatttgcccg acgaattggt
    26401 gcccagcaac atggtgacgg gctttagtgc cagatctccg gtatcgcgcc aggccttgaa
    26461 gttgataagt cgtaggcggg taaacatcag cgcagttctc cggtttccag tcgatcccag
    26521 gccgcgagca ccaggcgccg ggtgcggtat tcgccatatt ggcggatttc cttgtccttg
    26581 agcacgcgga aggtttcgga cgggcagtcg gcgtccatca cctcggcggg gccgaggaag
    26641 taggtcgccg ttttcatgcg tcagcccagc agccgacgtt gcaacagcgt ttgcatgtct
    26701 tgccgaccgt cggcgatcag catgacagtg acggtgtcct gcccgacccg gtagaccatc
    26761 cgccgcggct tgtaatgagt ctggcgaaac tgccggacgt cgagcgcggc cagctccggc
    26821 gggtattcac cgcgctccgg cagctcgcgc agcgcggtga gtttttcgtg gatccgctcc
    26881 aacacgccat cgccgagctc gatgccgtcc tgcgcggcga taaaggcgta caggtcatcg
    26941 agatcgcgca gcgcatcctc ggtgagaagg atggcaaagc tcattcgctg ccctggtgac
    27001 gcgccttcac ccgggcgacg gcgtcatcga gcggcacgac cctgccctcg gcgatctgct
    27061 gctgaccgag cgccagaatc ttgagaagcg ccagcgtctg ttgtgtttgc tcgtagctgt
    27121 ggatgtcctg caccacgggc cttggcttcg ccgttcaggg tggtgaccat cgcgctgccc
    27181 tcctcgccga gctggcgcac gacctcggag gcgtgggcct tgaagtagct caaggggcga
    27241 atatgggtga cgagcttcat gtcagtgcct cgtcatgatg gctgaattcg gtccgattat
    27301 aggtcgcccg gtgacgattg gggagccgag ccggagtctt cacccgacca gcttgaccag
    27361 ctttccgaag gcgagcgccc actgggcgtg ctcctcgccg aagcggacga aggtgtagcc
    27421 ccggtcttcc cattcggttt gcaggtggcg cggcgtatcc ggcagggcga cgggcactgg
    27481 tggtcgggcc agtgcagcag cgggctgccg gcgtgctcgg cggcaaccgg cggcagcagg
    27541 ccactcgttt cggcggcgaa gccctgctgc caggtcgcct gcccgccttt ggcttcattc
    27601 atgtctgctt ttgcgccgga tggcgactgc ggcggggcag tctcggccag cgcggtttcg
    27661 gagcaggtca ggcgccacag gatgcggcgg gcgtgttcgt cgcgccggtc cagcagttcg
    27721 gttccggctg gttgtaatag tagagcaggc agcggtagca gccggcgacg caggcggtgt
    27781 cgggcaggtc cggcaagtca gcggggcgcg ggtgaccagg tcagcaggtt ttccggcatc
    27841 gcgctggagt ttgcttgctg gcttgattgt atcttcttgg aaggccgagc gttgcactga
    27901 caggcgcgtc gaatcccatc gcctttcctg gcaccttcac tctacggcca tgctttccag
    27961 agagcgctgg ctggcggtgt cgtgatgctc atgcgttttt gccatctccg ggcgagcggg
    28021 tggaagatcg ggtgccgcgt cggcgggaag tgagctgctc tcttccagca tgtccagagg
    28081 cctgatgccg atggcgtggt agcccttggg acccttggag atctcgaaac tgactctctg
    28141 gcccgccttg agggtcttgt atccatccat gatgatggcg gagtagtggg cgaacatgtc
    28201 ttcagagcgg ttgtccgcaa tgatgaaacc gtagcccttg gcgttgttga accatttgac
    28261 atggcccagg ctgattgtcg acatcacaat cgtctccttt tttattattg cgcacgggtt
    28321 ttcccgatgc tgttatggcg gacggtcgtt cagcgcggat ccatgcgctg catggatcaa
    28381 aaatgttttc tggctgcttt tggccgggaa tggcttgcct tgtcatggcc ggaactccat
    28441 ctccgcgcga gccaaacctt aatcgaaggt ggctggcaaa tcaatttctt gaagcgtgtc
    28501 atcggccaga gctgtggagt ggttctgacg gcatggtgac ggtccatgga gccttgcccc
    28561 cgtgcagcgt cagctgagcg cgaccgttgc cctgttctga ttgcgctcca ggagtgatct
    28621 ggcatcgctg ggatccagcg agcgactcag cagaaagccc tgtgcctcat ggcagcccag
    28681 gcggcgcagg taatccagct gctccgcggt ttcgacgcct tcggccacca gattgagttt
    28741 cagccccttg ccaatggatg caatggcatc gaccaggcag gcatccgggt tggtgacgtt
    28801 gatctcgttg atgaacacgc gatcgatttt caaggtggtg agggggaatt tctgcaggta
    28861 actgagcgat gaaaatccgg ttccgaaatc atcgagcgcg atcttgacgc catgctgatg
    28921 cagcgtttga agtttggcga cgacgccctc ccaatccttc atcagcagat tttcggtgat
    28981 ctccacctcc agcaggctgg ggtcgagtcg atgctcttcg agaacggcga gcaccagcga
    29041 ggtaaaattg ggttgctcga tctgcaggac ggaaaagttg atgcccatgc ggattggctc
    29101 cagccctgca tcgagccact cacgcaaggc ggcgcagccc tgcctcaata cccattcgcc
    29161 gagcatcagc accaatccgc tctcttcggc gatgggaatg aactcgttcg gcagcagcag
    29221 tccgcgcagt gggtgacgcc agcgcaccaa tgcctccatg ccgaggattt ttcctgaatc
    29281 gatgtccatc tgtggctggt actgcacgca gaactgctgg ccctcgatgc ctcgacgaat
    29341 gtcgttttcg agcagcaacc gttccgaagc ggctgcatcc atgctcgatt cgtaaaagca
    29401 gaagccattg cggttgcgct ttttcacctg atacatcgcc agatcggcat ggccgaccag
    29461 ttggttcatc gaggtgccgt cgcggggata gagcgcgatg ccaatgctgg caccgatgaa
    29521 gatttcgtgg ccatcgagca gaaacgggct ggcgaggcga gccatgatct tgccggcgat
    29581 ggtggcggcg tcctgctccg agtgcaggct gggcagcagc agggtgaact cgtctccgcc
    29641 aaagcgtgcc agcgtatcgc cttcgcgcag cgatgagcgc agtgtttcgc tgaccgcctg
    29701 cagcagcagg tcaccggtcg cgtgccccag cgagtcgttc accaccttga aacggtccag
    29761 gtcgatgaac agcaccgcaa agatatcttt gttgcgtttg gcgtgggcaa cggccatctc
    29821 cagtcgatcc ttgaacaggg tccggttggg cagcccggtc agcagatcgt ggtaggcctg
    29881 aaactgaatg gtctgttccg cctgtttgcg ctcggtgatg tcgcgcgcaa tgccgaaggt
    29941 tccctgggtt gtggaggtga tttcactgat gctctggtcg atcacgcctg tttgggagaa
    30001 atcgagcggg aagtactcga catagagatg gcgtacttgg tggaatttgt tcctgacccg
    30061 aatttcagcc tgctttctga atgaggattc gacatcgcca tggccggtat cgcatgacaa
    30121 ctgctgccac tcctcttcgt cgtccagcac gacagagaag tgctggccaa gcagtgcctc
    30181 tgtctgatag ccaagcaggt cggaaacgcg gttgttgatg aaggtgaaac ggccttcatg
    30241 atcgaggaca taaatgacgt cgggagagtg ttcgaccagg tggcggtgca gtttttccga
    30301 ctgccacagc cgttgttgaa acagccggtt ttcggttttc agccgttgat tggtcagcgt
    30361 attggagaga gtcttgagca gttcatcgct cgagtagggc ttgcggatgt aatcggtggc
    30421 gcccagcaac agcgcctggg tgatggcgcc gaatgaagat tcgccgctga tgacgatgac
    30481 cggcgtctca tactgttggt cggcaatgtg ctgaagcaca tcatgcccgc tgatgtcttc
    30541 catgcgcagg tcgagcagga tggcgtcgaa gtggtcgtta tcgagtcgct cgatcgcctg
    30601 tcgaccaccc tgggccagtt cagtctggta tccatgcagg acgagcagtt cttgcaggct
    30661 ccgcagcaga cggatttcat catcgaccac cagcaacctg gcagcgtcgg gctgcttgcc
    30721 ggagatgctc atctgcattg tcgtgccacc ctgcggacgc tctgctgtga tgaatggatg
    30781 ttctccgtct ggaaacggag atcgcctggc cgtcctggct cttgtgactc ctttgagctg
    30841 cgctccctta catgtcgatg ttcagtggta caaagcgaaa aagtggttaa taaaaaatac
    30901 ggcacactcg atgtcaatcg agtgacgtgt cttgctgaag tcgcggggac ctgtctgagg
    30961 tgccgatggg tgacggtgca tggaaactgc attggcctgc atgaaattga tgcgtctttt
    31021 tttgttatct ggcgaggtaa aatgctcgac ccttcaaatc ggactgtctt tttttgcgca
    31081 tctttttcag aaccataaca aagacggatg accatgtcca gataaagcat ttcgttgtcg
    31141 agacaagcat gcgtgcctgt catcagcagg ggtggggcag gcgcaatctg acatcaccaa
    31201 gaaaaaaacc atgcggggag tgcatcctgc gcatctgcgg tcatgatgga gatcgaaaat
    31261 gcgccggccg attattctgt ggcgctcccg agcgagaaaa aaataccgga actggtgata
    31321 cccaattgag gtttgccgtc caccatgcgt tcttcggcca tttcgatcag acgataaaag
    31381 agtgcgcggg tcagccgtcc atgaaggcgt ccgcgaatcg cgaggtaggg cgatggttca
    31441 ccggtggcgc tctcttcgac ccacaggggg tggtcggggc cggccagcac ctggtcgccg
    31501 acctgggtgg tgaagcagaa gcgccgttga tcgccttcgc ccgccacttc cacatcggtg
    31561 accagaaagt gggcatcctc gacctcgatg cgcagtcgtt cggccggtgt caccagatag
    31621 ctgtggccat cttcatcgcg acgcaggatg gtcgagaaca gccgtaccag ctccttgcgc
    31681 ctgatcggtt cgccttcgtg gtaccaggtg ccgtcacgcg caatgcgaat gtcgatctcg
    31741 ccaacccgcg ccggatgcca ctgatcgacg ggcggcaagc tggcaggagg tttgaagtgg
    31801 tgaatctgtt gggccagatg ttccactgtg ggatcggagg tgttcatgtc gggagtctca
    31861 tgggtggctg acgcaaggcg gggaaattct cctgtgagcc gggccgccaa tgcaatggat
    31921 cgatgccggc aggcttgcgc tgactgtact ggatggcaga agaagggtgg ctggcctgat
    31981 ctacgggagg gtcaccaggg cagaggagag agattgacgg ggatcgcctg catggtggat
    32041 gctaaccagt cgaatatttc cgttcggtag ccgtcggtgg gccagcgtcg gcggacatgg
    32101 cgtcatggag ggcgatgtgc ttttcgcccc gccgcgttcc gccccttggt caatgccgca
    32161 tgacataaca ataaaacagg agggagagcg aatggcgctt gattttgcga cgcgtcggtt
    32221 tctggaaatg atggctgaac ggggccggcc gccgctgcac gcgggaacac cgaccgaagc
    32281 gcggttggcc agcgcggcga tggccgatct ggcgggcccg gcaccgccga tggtgcgagt
    32341 cgaagaccat tcggtggtca gcagcgatgg cggttcggtg ccgatgcggc tggtggtgcc
    32401 gatcgagtcg ccgcgcggat tgatcgtctt ctaccatggc ggtggctggg tgatcggctc
    32461 ggcacagcag agcgagaccg tcggccgcaa gctggcggag cgcacctcct gtgcggtact
    32521 gctggtcgac taccggctgg cgccggagca ccgctatcca attgcggtcg aggactccta
    32581 cgcggcgctg ttgtgggccg atgcgaacct gcagcggatc gccggccgca gggtgccgtt
    32641 gatggtggcc ggtgacagtg ccggcggcaa tctggcggca gtgatggcgc tgcgcgcccg
    32701 cgaccagcag ggtcctgcct tggcgttgca ggtgctcatc tatccggtga ccgatgccga
    32761 tttcgagcgt gcttcctacg tcgatccgga aaaccagctc tttttgtcgc gtgagtcgat
    32821 gctctggttc tgggatcact atctgccaga cccggcacgg cggctggagc ccgatgcctc
    32881 gccgctgcac gcggccgatc tttccggatt gccgccggca gtggtgctga ccgccgagca
    32941 tgacgtgctg cgcgatgagg gcgaggccta tgcacagcgc ctgcagcagg cgggtgtcgc
    33001 cgtcgatttc aagcgccatg ccggccagat gcatggtttt ttcaccgtac tggccctg
//
LOCUS       DAORCQ010000019        33023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_707_length_33023_cov_6.191883, whole genome shotgun sequence.
ACCESSION   DAORCQ010000019 DAORCQ010000000
VERSION     DAORCQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 33023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33023
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_33023_cov_6.191883"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKI23_03320"
     CDS             complement(<1..379)
                     /locus_tag="PKI23_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNN36004.1"
                     /translation="MPRRARITLPNVPLHLIQRGNNRQACFFADEDYRFYLDWLAEYA
                     EKTDCRVHAYVLMTNHVHLLLSPGHAGAAGALMKALGQRYVQYVNRIYRRSGTLWEGR
                     FRSCPTQEETYLLACQRYIELNPV"
     gene            534..836
                     /locus_tag="PKI23_03325"
     CDS             534..836
                     /locus_tag="PKI23_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNN36005.1"
                     /translation="MIEIRKTDLFTEWLDGLQDIQARARIQARIERLAAGNPGDVESV
                     GEGVSELRINMGPGYRVYFRQRGRELIILLAGGDKRTQARDIKTALRLARNLLESQ"
     gene            837..1136
                     /locus_tag="PKI23_03330"
     CDS             837..1136
                     /locus_tag="PKI23_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008038711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HNN36006.1"
                     /translation="MTKITTTRYDVAEHLRTPEEMAAYLEACLEEADGDAAFIAKALG
                     DIARAKGMAQVARDAGLSRESLYKALSGERSPSFDTILKVIGALGLKLHAEASQR"
     gene            1321..1554
                     /locus_tag="PKI23_03335"
     CDS             1321..1554
                     /locus_tag="PKI23_03335"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HNN36007.1"
                     /translation="MSTSTSIRIDQKLYEQARSEAVSEHRTIAGQIEYWAKVGRAALD
                     NPDLPVSFIAESLAALAESRTEVTDFAPRSRRA"
     gene            1551..1832
                     /locus_tag="PKI23_03340"
     CDS             1551..1832
                     /locus_tag="PKI23_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNN36008.1"
                     /translation="MTYGLKQTRRFARAYKKLHDNVAADVDAATEVIAADPAVGERKK
                     GDLAELRVYRFRSQNQLYLLGYSIDDSVRLVYLEAVGPHENFYRELKRS"
     gene            1863..1994
                     /locus_tag="PKI23_03345"
                     /pseudo
     CDS             1863..1994
                     /locus_tag="PKI23_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003221244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(2050..3576)
                     /gene="ppx"
                     /locus_tag="PKI23_03350"
     CDS             complement(2050..3576)
                     /gene="ppx"
                     /locus_tag="PKI23_03350"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNN36009.1"
                     /translation="MTETPPLQHELIDSPTQYAAIDLGSNSFHMLIAELVEGELRPLQ
                     RFREKVRLAEGLDDHRLLDEAARERGFQCLRRFAQRLRNMPSERVAIRATNTLRVAIN
                     ANDFIAVAEGILGHPVEVISGVEEARLIYLGVAHTLADDAGRRLVVDIGGGSTELIVG
                     ERFEPQVLESLAVGCVAYERRFFANGELSEARFKRARLAACSELVEIADELKPFDWSS
                     VVGASGTVRAIARILQQNGWSDDEQIGRDGLEKLRMALLKCSHVGQIRLAGLERERAS
                     ILPAGLAILTAIFDTLGIAQMRYSDGALREGVLYDLIGRSSHEDVRARTIDALSRRFR
                     VDRPQARRVERTVLALHDQVVERWALHDPRCRRWLGWAAQLHEIGLAVSHSQFHRHGA
                     YLVRNVDLPGFTQQTQQMLALLVRGHRRRFPAADDFAVLRGQGDVARRLVLLLRLAVL
                     LNHGRAAHEVPRLRIEVDGPAILLRFPPRWLADHPLTQADFDNEAQLLRPAGFELQIC
                     "
     gene            complement(3599..4663)
                     /gene="murB"
                     /locus_tag="PKI23_03355"
     CDS             complement(3599..4663)
                     /gene="murB"
                     /locus_tag="PKI23_03355"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNN36010.1"
                     /translation="MKVDIVEQVDLTPLNPLALPATSRHFATVDSLAALQWLLSIARE
                     RHWPLLLLGGGSNLLLRGDHPGLTLKMAFDEIRLHQQGDQLWVRAGAGVVWDRLVETV
                     VAADWHGIENLSGIPGTVGAAPIQNIGAYGAELADCFDHLIAVDATSGAVHRFERDQC
                     QFGYRDSLFKRNGQWIIVEVTLRLDRAMSPRSDHGAVQSELRARGITAPSVADLRRAV
                     LAIRASKLPDPRQLPNAGSFFHNPVVSAAHHARLRRSHPDLVSHPLPSGDFKLAAGWL
                     IERLGWKGVRQGPVGVHPQQALVLVNHGGATAAQLLALAGQISDQVRQLFGVTLTIEP
                     VCVPPLPLPLPGLPHAALAT"
     gene            complement(4701..6212)
                     /locus_tag="PKI23_03360"
     CDS             complement(4701..6212)
                     /locus_tag="PKI23_03360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNN36011.1"
                     /translation="MPTCEPLTRPAARPWRAWWLLLWVLLGGSSGCATNPVSGQSDFV
                     MMSEAEEVAIGRQYHPRILKEYGYYDNEALQEYVQQVGGKVAAMSHRPGLVYRFTVVD
                     SPTINAFALPGGYIYITRGLLAYLDSEAELAAVLGHEIGHVTARHAVRQQSAAQAAGI
                     GYTVAQILLPELRSSAAQDMFNILAQATLSGYGREQELESDRLGAEYLARSGYPPQAM
                     LDVLRVLKAQEEYGRQLAQAEGRDPQSVGGYHGLFASHPDNDTRLKEVVNEALPLYVD
                     RPAADRGVESFLKQIDGLVYGDSPEQGVRRQNRFYHARMGIAVEFPTGWEVQNLADRV
                     VAVAPSGEATQVMTVQDLNRAMPPEQFIAERLRLQLSDVQPLAVEPLQGVTGRTVVST
                     RQGKRPARVSVIYKGPQAFILLGTSRELADFELYDGKLQQSAASFHALTPDERALAQA
                     LRIRLIRADDGTRYATLAQQSILPGLKEAQLRLLNQHYPAGEPRTGQMLKVVK"
     gene            complement(6199..7122)
                     /gene="lpxL"
                     /locus_tag="PKI23_03365"
     CDS             complement(6199..7122)
                     /gene="lpxL"
                     /locus_tag="PKI23_03365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HNN36012.1"
                     /translation="MAKSKERGSLYHPRHWPSWLVIGLWYLSSFLPYRWLYQLGRPIG
                     WLLFALGRSRAAVGRRNLALCFPELSPAQRERLLKRNFEQMGITLMETGFAWWASDRR
                     IDRISRVEGLEQLIERQQRGEGALLISFHFLAIDLTARIVGRHGDFHALYKIQGNRVF
                     EAVSKRIRERHHVYIIPHKQVQELLDRAHSGKLVAVVPDQDFGPRRSLFVPFFGIATA
                     TIPNVSDYARQTGFPVFPLTFLRDADGRGLSIKVYPPFDDFPSGDDFADTLRMNRFYE
                     EIIRDHPQQYLWVHRRFKTRPPGEESLYADL"
     gene            complement(7268..8362)
                     /locus_tag="PKI23_03370"
     CDS             complement(7268..8362)
                     /locus_tag="PKI23_03370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36013.1"
                     /translation="MEFGLSSEQKLLSETVRRFVEQQVPLDRVRQIAAERSGFDQAIW
                     QGLADLGVLGIIVPEEYGGAGLSFFDAALVQEHLGWAVTPAPYSSTALMAPVAFLHAA
                     TEMQKQVWLPKIAAGEVKIGVGLSEAVGAREGAGLTLKEGKLHGKALFVLDAMVADLL
                     LLAVGRDTLVLVPANEAGVTRNALNNIDRTRGLAELLFDGATAIPLGSVGGAATAIRK
                     TIDAGRVALAADNLGAAQRMIEKTVVYSLERKQFNRPIGSFQGYKHMCAEMAAELEPS
                     RSLVWYAAHCQSEIPNEASVMACHAKAHLAEVCTEIANKATLGHGGMGFTDLMGVHYW
                     FKRIGYNRQILGGPEAVRADAAALQGLVAA"
     gene            complement(8381..9577)
                     /locus_tag="PKI23_03375"
     CDS             complement(8381..9577)
                     /locus_tag="PKI23_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36014.1"
                     /translation="MDLRYGKEYEDFRQEVRAFLAKHGHKSPSLQGEMGRPTAQAREW
                     QALLIEQGYAARTIPRQYGGYGGEPDVLKSVIVDQEFDQAGVSKGLSGQGISMLVPTL
                     LELGTEEQKQRWIRPTITGEIFWCQGYSEPNAGSDLASLQTRAHVEGDEFVINGQKIW
                     TSSAHFSDMMFVLVRTEADAPKHMGISYLLLPMTTPGIEVRPLVTMTGDATFNEVFFT
                     DVRLKTDQIVARRGDGWMVANATLKHERGMLGDPSQSANLLHQVTELLTSETVDGVRL
                     IDSPLYRDRLMRLQARVASMQWHSMRLLTNTLKNELELMPILVVKLIGTHLNQDICAL
                     AIDALGELGILYDQSKYLRAMGMWQSRYMFSLGLIIGGGTSHIQKNIISERGLGMPRE
                     PKAVSA"
     gene            complement(9734..10630)
                     /gene="rsmI"
                     /locus_tag="PKI23_03380"
     CDS             complement(9734..10630)
                     /gene="rsmI"
                     /locus_tag="PKI23_03380"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNN36015.1"
                     /translation="MSGTTAVSFEGLLAPALYIVATPIGNMGDITLRALEVLRGVDLI
                     AAEDTRHSRGLLSHFGISTPVTAYHEHSSERVRERLLARILAGERVALISDAGTPTIS
                     DPGYRLVKRVAEAGGRVIPIPGANAAIAALSVAGLPSDRFAFEGFLPSREEARSAQLQ
                     RLANESRTLLFYEAPHRLVATLEAMVAAFGAERSAVVARELTKLHESVRRATLAQLAE
                     RFRGGEESARGEVVIVVAGCEREVTLSQQQAERMLRELLTELPLARAARLASRMTGLE
                     RDWLYARGVAWQRGDDADRSEK"
     gene            10660..12531
                     /locus_tag="PKI23_03385"
     CDS             10660..12531
                     /locus_tag="PKI23_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNN36016.1"
                     /translation="MPCSTLQFRSARPGAWLLALLWLLTAAGCVSTRPVEPPPGTPEQ
                     LTVEALLKQAEKLSSPQREEYLILAAEQRLQQQQIAEAEQLSSPLDIGLLPLDGRRRL
                     ALLRAEIALDGNQLDTASNWFNLPSAQFEQLSDASTLARLSLLRARWFEAKDQPLLAA
                     RERILADPLLAETARGDNHRQIWTLLGRVNPDRLRATARALPDNDLRGWLELAALDRQ
                     FTEANPQRLAALADWRSRWPRHPAAIEPPAGIADTLRPLEAAQQQGHARVLLLLPLSG
                     PLAAAGEVVRDGFMARYYQQLAAQQPLPELVLIDSAATPQASWLPQAQALKVDAIIGP
                     LPKEMVEALNQQPLDIPVLALNYLPDGVTPQPNLHQFGLANEVSAQLTARQASSAGQR
                     RAALLFADSPWSRRLERAFRTEWHKLGGEVVASASFNEATADHAQAVRQLLQVTAPAA
                     PTVAGSATAPVDGTAATASRRQDIDCIVLIAAPEQGRLIAPLFRLYFAGDLPLYGVTT
                     LYAGRSNPRQDSALDGIIFNDIPWLVDPAFTERAEIAALWPAAYPDHARLYALGRDAL
                     DLLPALEQLKAPGAELHGATGLLRLDADRRIQREQVPTRFENGVVVRLPAAGSGPAR"
     gene            12528..12920
                     /locus_tag="PKI23_03390"
     CDS             12528..12920
                     /locus_tag="PKI23_03390"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNN36017.1"
                     /translation="MKLFDRQRNRRIGAEAEASALDHLSRAGLRLVARNYRCRGGEID
                     LIMRDRQTLVFIEVRYRSGGRFASAAESVDASKQRRITTAAHHYLQRQPQYRDLPCRF
                     DVVAFDPAAQQGPALQPRWIQDAFHSTH"
     gene            12924..13520
                     /locus_tag="PKI23_03395"
     CDS             12924..13520
                     /locus_tag="PKI23_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HNN36018.1"
                     /translation="MNTQQRIFDLFQESIATKQAALELLGPSIELAAQMMVSCLLQGG
                     KILSCGNGGSAGDAQHFSSELLNRFERERPGLPAIALTTDSSTLTAIANDYSYHEVFS
                     KQVRALGQAGDLLLAITTSGNSPNLLQAVGAAHDRGMQVVALLGKGGGELRNLLGEVD
                     VEICVPSEVTARIQEVHLLVIHCLCDLIDNQLFGSIEM"
     gene            complement(13554..13937)
                     /locus_tag="PKI23_03400"
     CDS             complement(13554..13937)
                     /locus_tag="PKI23_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNN36019.1"
                     /translation="MSSQRPYLLRAIHQWILDNGCTPYLVIDADAPGVQVPRQYVNEG
                     RIVLNVAPGAVNALIIGNSEIDFDCRFGGVSTHLTLPLHAVRGIYAKENGQGLIFDPN
                     DEEPPPGGPDRTAGKSSPRHLSVVK"
     gene            complement(13983..14585)
                     /locus_tag="PKI23_03405"
     CDS             complement(13983..14585)
                     /locus_tag="PKI23_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNN36020.1"
                     /translation="MTFLSGATDHYSHRVRIVLAEKGVTVDLIELTEETKPTDLVEIN
                     PYNTLPTLVDRELVLYQPNIMMEYLDERFPHPPLLPVYPVARAQSRLLIYRIERDWSP
                     LADRILGGEEKEATLNKLRKELRDSLVGVAAIFEEAPFFMSEEFSLVDCCVVPLLWRL
                     PLMGIELPIRQIKPMAKYMERMFERESFQASLTDQEREMR"
     gene            complement(14785..15567)
                     /locus_tag="PKI23_03410"
     CDS             complement(14785..15567)
                     /locus_tag="PKI23_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNN36021.1"
                     /translation="MKKILKILILGWLPLLAWASGEEVALDHVKIDPADRHALQRGAA
                     LYMNYCMGCHSLKYERYEKLSLGLGVPPELFMKHLVFGQAKIGDLMTNAMPPANAKRW
                     FGATPPDLTLVTRLRGSDWVYSYLRAFYTDPARPWGVNNVVFKDVGMPNVLRELQGEQ
                     RMVCKQVPQLAENGGIKRDPLSGQNITVEDCHQLQVVEGSGKLSAKQFDTAVSDLVNF
                     LAYVAEPEKLERHRLGVYVLLFLAFFFVVAYMLNREYWKDVH"
     gene            complement(15567..16778)
                     /locus_tag="PKI23_03415"
     CDS             complement(15567..16778)
                     /locus_tag="PKI23_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNN36022.1"
                     /translation="MSGLIGWVDERLPIVEAYERHMSKYYAPKNFNFWYFFGVLAMLV
                     LVNQLLTGIWLTMSYNPSAEGAFASVEYIMRDVEWGWLLRYMHSTGASAFFVVIYLHM
                     FRGLLYGSYKKPRELIWIFGMMIYLALMAEGFFGYLLPWGNMSFWGAQVIVSLFTAIP
                     YIGEPLAQWVRGDYLISGITLNRFFALHVVALPIVLLALVVLHLLALHEVGSNNPDGV
                     EIKKLKDKDGIPLDGIAFHPYYTVHDIVGVVVFLAVFCSVIFFFPEMGGYFLEFANFE
                     PADPLKTPAHIAPVWYYTPFYAMLRAVPDKFGGLVVMAGAIAILFVLPWLDRSPVKSI
                     RYKGLLSKLFLWVFGISFLILGVLGAMAPTPQRTLLAQVCTVLYFAYFFLMPFYTRLE
                     KTKPVPQRVTG"
     gene            complement(16790..17389)
                     /gene="petA"
                     /locus_tag="PKI23_03420"
     CDS             complement(16790..17389)
                     /gene="petA"
                     /locus_tag="PKI23_03420"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNN36023.1"
                     /translation="MSSEGEVNAGRRRFLIGATSVVGAVGVVGAAVPFVGSWNPSAKA
                     KAAGAPVKADLSKLEPGQMVVVEWRGKPVFVVRRTKEALDQLTKMDAVLADPAGAVTS
                     QQPESIKSPYRSIEPEYLVVLGVCTHLGCSPKFRPELAPADLGADWKGGFFCPCHGSR
                     FDLSGRVYKGVPAPTNLEVPPYSFVGDKVILIGSEEGAA"
     gene            complement(17680..18072)
                     /gene="rpsI"
                     /locus_tag="PKI23_03425"
     CDS             complement(17680..18072)
                     /gene="rpsI"
                     /locus_tag="PKI23_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNN36024.1"
                     /translation="MAQTHYYGTGRRKTAAARVYLRAGSGTITVNDRPLDQYFGREVA
                     RMVVMQPLEAANLAGRFDAKISVSGGGSFGQAGAIRHGIARALVQYNEDLRGTLRKEG
                     HLTRDAREVERKKIGLRKARKRPQYSKR"
     gene            complement(18108..18536)
                     /gene="rplM"
                     /locus_tag="PKI23_03430"
     CDS             complement(18108..18536)
                     /gene="rplM"
                     /locus_tag="PKI23_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNN36025.1"
                     /translation="MKTLSAKPAEVRREWLLVDADGKTLGRLASEIAQRLWGKHKPIF
                     TPHVDAGDYIVVINAEKIRVTGSKRTDKTYYRHTGYPGGLKSSSFEEMIGSHPERVLG
                     AAVKGMLPKNSLGQAMFKKLKIYAGSEHPHAAQQPLPVDL"
     gene            complement(18603..19439)
                     /gene="xthA"
                     /locus_tag="PKI23_03435"
     CDS             complement(18603..19439)
                     /gene="xthA"
                     /locus_tag="PKI23_03435"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNN36026.1"
                     /translation="MRVISFNINGLRARLHQLAAVIERHRPDIIALQETKVSDAEFPQ
                     AAIDALGYHVVYHGQKGHYGVALLSRVAPRQVECGFSGDGAEAQCRLIRADYPLADGG
                     ELSVINGYFPQGESRDHPVKFPAKQKFYRDLRQLLDADYDPARRLIVTGDLNVSPADI
                     DIGIGEENRKRWLRSGKCSFLPEERAWFAELLVWGLQDSYRVLHPDRDDLYSWFDYRS
                     RGFEQQPRRGLRIDLLLATPGMIDRLQQVGIDYDIRGMERPSDHCPVWASFDCGVGVQ
                     EG"
     gene            19529..21043
                     /locus_tag="PKI23_03440"
     CDS             19529..21043
                     /locus_tag="PKI23_03440"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNN36027.1"
                     /translation="MQAPMHWFTTLLDLLAVLFIFMLGALCLTLLAWYFIDRHQKSHA
                     IRRNFPLIGRFRYLFEWLGEFFRQYFFAMDREELPFNRAERSWVYRAAKNLEQTAAFG
                     STNNLATPGALFFVHSTFPTAAAEPPAPVVIGPDTPHPYQADKFFNISAMSYGAISRP
                     AVLALSRGAALAGCWLNTGEGGLSPYHLEGGADLVFQLGTAKYGARDPQGNLSEEKLA
                     RIAAHPAVRLFEIKLSQGAKPGKGGILPAAKVTEEIAAIRGIPMGHDSLSPAGHLDAR
                     DIDGLLTLIDRVRRATGKPVGIKACIGESSWLDQLCIELNCRGFDSAPDFITVDGAEG
                     GSGAAPQSLLDYMGLPIRESLPLVVDTLVTHGLRERIKVVASGKLINPVEVAWALCMG
                     ADCINSARGFMFALGCIQSLKCNQNNCPTGITTHRIDLQQGLDPTDKAERVRHYQQNL
                     EREVAVIAHACGVESPRQLRRHHARMVVEAGRSVSLHERYPPAVIPVTAVGADR"
     gene            complement(21009..22028)
                     /locus_tag="PKI23_03445"
     CDS             complement(21009..22028)
                     /locus_tag="PKI23_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019362729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HNN36028.1"
                     /translation="MQSGESNRSSPLRRTLIALPIGLLLLVWTGLSFYWSRKPALFDV
                     RAEAATQLAQLGGPTTPPPGTVVTATAIGIGTRLLDKPGGYLGNDLFPPGVWLDDIAN
                     WEFGALTQLRDLTEAMRESFSRSQSQSAEDRDLVIAEPQFKFDNASWALPSTEGEYRQ
                     GLRALRSYLQRSSDDNHNDGQFHARADNLNFWLATVETRLGSLSQRLSASVGQVRIDT
                     TSGGTVDATAAAPAAVEQMTRTPWLQIDDVYYESQGSAWALLHILKAVEVDFAEVLQK
                     KGAQVSLRQIIRELEATQRPLTSPMVLNGDGFGLFANHSLVLASYISRAHAAIIDLRQ
                     LLSQG"
     gene            22180..25017
                     /gene="uvrA"
                     /locus_tag="PKI23_03450"
     CDS             22180..25017
                     /gene="uvrA"
                     /locus_tag="PKI23_03450"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNN36029.1"
                     /translation="MDKIVVRGARTHNLKNIDLELPRDQLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDHIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPRHQAPLNAQTVSQMVDQVLALDPERRLILLAPVVQERKG
                     EHLQLLETLKAKGFIRARINGSVVELDEAPLLEKNRKHSIEVVVDRFRPRSDLQLRLA
                     ESFETALQLSDGIVKVLLDDGEELIFSARFACPHCGYSIKELEPRLFSFNNPAGACSS
                     CDGLGVKQSFDLQRIVPNPELSLSEGAIRGWDRRNVYYFHLLSSLADHCGFSLDLPFA
                     KLKKRHQEIILHGSGDEQIDFRYMNDRGDLFKRRHTFEGILPNMERRYRESDSLSVRE
                     ELAKYLSSHPCGDCGGARLREEARHVFIHDWNLPAVSRLPVAEALHLFDTLTLQGQRA
                     EIASKIIKEIGARLRFLTDVGLGYLSLDRGAETLSGGEAQRIRLASQIGAGLVGVMYI
                     LDEPSIGLHQRDNERLLRSLFHLRDIGNTVIVVEHDEDAIRQADHLIDIGPGAGLHGG
                     QVVAQGSVAEIIANPASITGDYLAGRRHIAVPARRVAPQAERWIRLRNARGNNLRGID
                     VAIPLGVMTCVTGVSGSGKSTLINNTLYPLAATRLNGAGPLPIAPCDGIDGLELLDKV
                     IDIDQSPIGRTPRSNPATYTGLFTPIRDLFAGTQEARSRGYQAGRFSFNVKGGRCEAC
                     QGDGLIKVEMHFLPDVYVPCDLCQGKRYNRETLEILYKGRSIHQVLEMTVEEAREFFA
                     AVPMLTRKLQTLLDVGLGYIRLGQSATTLSGGEAQRVKLAKELSKRDTGRTLYILDEP
                     TTGLHFHDIQQLLQVLHSLRDEGNSIVVIEHNLDVIKTADWIIDLGPEGGDQGGTLIA
                     CGTPEQIATHPDSHTGHFLRPLLGEIRSAAG"
     gene            complement(25131..25829)
                     /locus_tag="PKI23_03455"
     CDS             complement(25131..25829)
                     /locus_tag="PKI23_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNN36030.1"
                     /translation="MSKDPNHQTRALLLGSAERESTALSVDSRRVLRNTYALLGLTLA
                     FSAAVAGLSMRLGLPHPGLLLTLIGFFGLSFLINRTADSGWGVLSVFALTGFMGYTLG
                     PLLSSVLSLPNGSAVVTNALGTTAVAFLGLSAIALTSKRDFSFMGKFLFIGVLVAFVL
                     GLGALFFEMPALSLAVSGMFVLLMSGMILHETSRIVNGGETNYVLATTSLFVSLYNLF
                     VSLLALFGLGGSDD"
     gene            complement(25871..26263)
                     /locus_tag="PKI23_03460"
     CDS             complement(25871..26263)
                     /locus_tag="PKI23_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36031.1"
                     /translation="MNVSICTQGIRLSREEHDHLHGRLRQLLARFGQRAMGVTLHLSD
                     VNGPRGGVDKECHLVVELVDTTAVVRDRGDHLLAVVDRVLHRAVQTIGRQIGKARDRA
                     VRAPQLGRHRAGGNRHGLQTLDDASAVT"
     gene            26365..27270
                     /gene="nhaR"
                     /locus_tag="PKI23_03465"
     CDS             26365..27270
                     /gene="nhaR"
                     /locus_tag="PKI23_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019411355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HNN36032.1"
                     /translation="MNYRHLQYFWAVARLGGVIRASEQLHVTPQTVSGQVQLLEEAIG
                     RPLFRKKGRKLELTETGELVYSYARDIFHLGSELEELLRQPRGVQRPVELRVGIVDAV
                     PKLVAGQLMTPAMVGAQPFRVICREGKIESLLADLSVHRLELVLSDRPMPPALSVSAY
                     THRLGGSHVGFYAANQLAATFDRPFPQLLDGAPLLSPGSESAMGLRLRQWLGKHKLRP
                     RVVGEFDDSAMVKSLGMQGLGVFIAPRVLDASVCRQYDATCLGEATDLIEEFHVITVE
                     RRITHPGVAAITEAARQGLFADWGR"
     gene            complement(27321..29162)
                     /locus_tag="PKI23_03470"
     CDS             complement(27321..29162)
                     /locus_tag="PKI23_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN36033.1"
                     /translation="MIIRHTDTFTAIRALRQVVEALSLEADFEAFFRKAAGAAADLVG
                     ADAAALILLNDDGQLEYQFFLGVPDGYSDKFLGYRFGVQAGVAGEALTHRRPVFVPDY
                     PAHPGAIAEFVASGLRANLAVPLHSAGEAVGVLVVSWFRNAVHAPDQALLSLLSTISA
                     QVGLAHHRWRLEQRLEHRANHDDLTGLPNRGHFLERLQQAVADGRRYGRRYALMVIDL
                     DGFKAVNDRAGHAVGDALLRDVAQGLREVVRGGDVIARLGGDEFVLLMEFQSWPEEPA
                     TVAARVKARLTLRVAVAGGTTAVTPSIGIAAFPEDGDDAGTLLANADLAMYEAKRRHG
                     EREVCFFNRSIAQAVRQREVLLEEVEQALNDGRFVLHYQPIVELPGGHMVAVEALLRW
                     PQPDGSLRPPVSFIPDVERHSRRLMVALGRYVLRSALAQAAAWHRQGHPIAVSVNVSA
                     REFLEEGFLASLTELLADHGDLPRERLMLEITETAALEDLPRAQALMQACRELGVRFA
                     IDDFGAGHASLANLRELPVDRLKIDRAFVAGLPDADGDRAVVQAILAVAKAFGVGVVA
                     EGVERAAQSQTLQELGCRVMQGYHFARPMPAALITERLDAERQASPK"
     gene            complement(29442..29807)
                     /locus_tag="PKI23_03475"
     CDS             complement(29442..29807)
                     /locus_tag="PKI23_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36034.1"
                     /translation="MELSELIAHYADAWSQPDRARRQQLLESVWDEQGTYADPFGLVA
                     GRMALVDHMGAFIQQYPGSRLERTSEIDAHDRYFRFNWRMLNADGTLFVEGIDFGELS
                     PTGQLQRLVGFFGALEAKG"
     gene            complement(29880..30749)
                     /locus_tag="PKI23_03480"
     CDS             complement(29880..30749)
                     /locus_tag="PKI23_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNN36035.1"
                     /translation="MQHNAADFPNQGVIPLFNLDADPPQAAHATPAMLTVNGQPIAPQ
                     RIAAEMQHFPGDQFESSWQQAARALIIRELLLQQARRMALDAIPQAEPQGGIETEEDA
                     LIRTLIEREVPPCEAPAEQVRRHYDDHPERFMTPLLHEAEHILVAARRDQADAFAAAR
                     AKAEQLHARLQQAPERFAELARAFSDCASGADGGRLGQLGPGETTPPFEAALQCLAPG
                     ELSAPLETAYGFHLIRLLRRGEPQRLPFEAVSATIAARLAEQAQRRAIARYIAQLIEQ
                     ADIQGFTPEPPLR"
     gene            complement(30760..31464)
                     /gene="narI"
                     /locus_tag="PKI23_03485"
     CDS             complement(30760..31464)
                     /gene="narI"
                     /locus_tag="PKI23_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNN36036.1"
                     /translation="MSATLNMTLFGWYPYLCLTIFLLGSLLRFDREQYSWKSGSSQLL
                     RKGQLRWGSNLFHVGILTILAGHFVGLLTPIEIFDQLGISHGFKQTLAIVVGGLAGVV
                     CFIGITLLAHRRLFDARIRATSSVGDIAILLLLWLQLTLGLCTIFVSLGHLDGHEMVK
                     FMAWAQGIVTLQPAAAVHVADVHPIFKAHLLLGLTLLLLFPFTRLVHIWSAPIWYLGR
                     PGYQIVRRRAQPPRNA"
     gene            complement(31484..32164)
                     /gene="narJ"
                     /locus_tag="PKI23_03490"
     CDS             complement(31484..32164)
                     /gene="narJ"
                     /locus_tag="PKI23_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNN36037.1"
                     /translation="MSLTFKALSALLCYPSETLQAAIAEIRGLIAAEGQIAPAVLAEL
                     EGFCAFIEQSDLIDLQEHYVALFDRSRQLSLHLFEHVHGESRDRGQALVDLAALYEQG
                     GLERVDNELPDYLPLFLEYLSTRPLDEAQSLLAETLQILAALEERLRARDSGYASLLR
                     AIRSAAGDQSLPTVAVNLASDLLAELDAEWAEPPVIFGPGAAACEGAGATVASVPLSA
                     VPRRARQT"
     gene            complement(32161..>33023)
                     /gene="narH"
                     /locus_tag="PKI23_03495"
     CDS             complement(32161..>33023)
                     /gene="narH"
                     /locus_tag="PKI23_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477584.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNN36038.1"
                     /translation="KIYYNWKSGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLL
                     YDADRIEAAASAADERDLYQAQLDLFLDPHDPAVIAQARADGIPESWLEAARHSPVWK
                     MAMEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAAAAGQIGIDAGLPDVRSLRIPLRYL
                     ANMLTAGDEAPVALALERMLAMRGYMRAKSVEGRIDESIAERVGLSGAVIEEMYRIMA
                     IANYEDRFVIPTARREAHEDLYALRGNCGFSFGNGCSGGSSKAELFTPVKLFSPAQGS
                     QSHNPGEVLK"
BASE COUNT         6022 a        11057 c        10419 g         5525 t
ORIGIN      
        1 gcaccggatt gagttcaatg tagcgctgac aggccagcag gtaggtctct tcctgtgtcg
       61 ggcatgaacg gaagcgccct tcccagagtg tgccgctgcg ccgatagatg cgattcacgt
      121 actgcacgta acgctgtccg agcgccttca tcagcgcgcc agccgccccg gcatgaccgg
      181 gagagagcag cagatgcacg tgattggtca tcagcacata ggcatgaacc cggcagtccg
      241 ttttttcggc gtattcggcc aaccaatcca gataaaagcg gtaatcctcg tcggcaaaaa
      301 agcaggcctg ccgattgttg ccgcgctgga tcaaatggag tggcacattc ggcagtgtga
      361 tgcgagcgcg gcgtggcatg gcagggctcc gtgggaaaat tggcctgagt ctagcagaaa
      421 accgtggtct gtccctattt tctccgtggt ctgtccctat tttctctgtc cctattttct
      481 ctgtccctat tttctccttt gacaaaacgt atccaaatgg ctacattttg tcaatgatcg
      541 aaattcgcaa gaccgacctc ttcaccgaat ggctggatgg acttcaggat attcaggccc
      601 gcgcccggat tcaggccagg atcgaacgtc tggcggccgg gaaccccggt gatgtcgaat
      661 ccgttggcga gggcgtttcc gagttgcgaa tcaacatggg tcccggttac cgggtctatt
      721 tcaggcagcg cgggcgcgag ctgatcatcc tgttggcagg tggcgacaag cgcacccagg
      781 ccagagacat caagacggcc ttgcgcctcg ccaggaacct tttggagtcg cagtagatga
      841 cgaagatcac gaccacccgc tatgacgttg cagaacacct cagaaccccc gaggagatgg
      901 ctgcctatct cgaagcctgc cttgaagagg cggacggtga tgcggctttc atcgccaagg
      961 cgctcggtga tatcgcccgc gccaagggca tggcccaggt cgctcgcgac gcgggcctgt
     1021 cgcgtgaaag tctctacaag gcgctctctg gcgaacgcag cccgagcttc gacaccatcc
     1081 tcaaggtgat cggtgcgctt ggattgaaac tgcatgccga ggccagccag cgctgacagc
     1141 gcgaaacggg gacactgctc aagattcact tgcctgatca atagccaaga acaggaaacc
     1201 gtggtctgtc cccaatttgg gcctttgtgc caatttgccc aatttgcctg cgcattgggg
     1261 agctaccgca tttgtagtaa tctcgtcgca gtgaacaccg ttctcagccg gaggcacaag
     1321 atgagcactt caacatccat ccgcatcgat cagaagctgt atgaacaagc ccgcagcgag
     1381 gcagtgagtg agcaccgcac cattgccgga cagatcgagt attgggccaa ggtcggacgg
     1441 gcggcgctgg acaaccccga cctgcccgtg agcttcattg ccgagtcgct ggccgcgctg
     1501 gccgagtcac gcacggaggt cactgacttc gcgccccggt cacgccgcgc atgacctatg
     1561 ggctcaagca gacccgacgc tttgcccgcg cctacaaaaa gctgcacgac aacgtcgccg
     1621 ccgacgtcga tgcagccacc gaggtgatag ccgctgaccc cgccgtgggc gagcgcaaaa
     1681 aaggcgacct ggccgaactg cgggtctaca ggtttcgcag ccagaaccaa ctgtatctgc
     1741 tgggctactc catcgatgac agcgttcggc tggtgtatct ggaagccgtc gggcctcacg
     1801 aaaacttcta tcgcgagctc aagcgaagct gatacgacgc ctgccgtgac cacagcccgc
     1861 gccaagggca tggccaggtc gctcgcgatg cggacctgtc gcgcgaaagt ctctgcaagg
     1921 cgctctgtgg cgaacgcagc ccgagcttcg acaccatcct caaggtgatc ggtgcgcttg
     1981 gattgaaact gcatcccgag gccagccagc gctggcagag gcactgcctt tcctctccca
     2041 tcccccgctt cagcagattt gcagctcgaa accggccggg cgcagcagtt gcgcttcgtt
     2101 gtcgaagtcg gcctgggtca gcgggtggtc ggccagccag cgcggcggaa agcgcagcag
     2161 aatcgccgga ccatcgacct cgatccgcag ccgtggcact tcatgggcag cacggccatg
     2221 gttgagcagc acggccagac gcagcagcag caccagccgg cgggcgacat cgccctgacc
     2281 gcgcagcacg gcaaagtcgt cggcggccgg aaagcggcgg cggtggccac gcaccagcag
     2341 cgccagcatt tgctgggtct gctgggtgaa accgggcaga tcgacgttgc gcaccaggta
     2401 ggcgccatgg cggtggaact gcgaatgcga caccgccagg ccaatctcgt gcaactgggc
     2461 agcccagccg agccagcggc ggcagcgcgg gtcgtgcagt gcccagcgct cgacgacctg
     2521 gtcgtgcagc gccagcacgg tgcgctcgac acggcgcgcc tgggggcggt cgacgcgaaa
     2581 gcggcggctc agggcatcga tggtgcgggc ccgcacatct tcatggctcg aacggccgat
     2641 caggtcatag agcacccctt cgcgcagcgc gccgtcggag tagcgcatct gcgcgatgcc
     2701 gagggtgtcg aagatggcgg tgagaatcgc cagacccgcc ggcaggatgg aggcacgctc
     2761 acgttcgaga ccggccagcc tgatctggcc gacatgcgag catttcagca gcgccatgcg
     2821 caacttttca agcccatcac gcccgatctg ctcgtcgtcg ctccagccat tctgctgcag
     2881 gatgcgcgcg atcgcccgca cggtgcccga tgcgccgacc acgctgctcc agtcgaaggg
     2941 tttgagctcg tcggcaatct cgaccagttc gctgcaggcg gccagccgcg cccgcttgaa
     3001 gcgggcctcg ctcagttcgc cattggcaaa gaagcgccgc tcgtaggcga cgcagccgac
     3061 cgccagactc tccagcacct ggggttcgaa gcgttcgccg acgatcagct cggtgctgcc
     3121 gccgccgatg tcgaccacca gccgccggcc ggcatcgtcg gccagcgtgt gggcgacgcc
     3181 aaggtagatc agccgcgcct cctcgacgcc ggagatcacc tcgaccggat ggccgagaat
     3241 cccctcggcc accgcgatga agtcgttggc attgatcgcc acccgcagcg tgttggtggc
     3301 gcggatcgcc acccgctccg acggcatgtt gcgcagccgc tgggcaaagc ggcgcagaca
     3361 ctgaaagccg cgctcgcgcg ccgcctcgtc gagcagccgg tggtcgtcga gcccctcggc
     3421 cagccgcacc ttttcgcgga accgctgcaa tggccgcagt tcgccctcga ccagttcggc
     3481 aatcagcatg tgaaagctgt tgctgccgag gtcgatggcg gcatattggg tcggcgagtc
     3541 gatcaattcg tgctggagcg ggggcgtttc ggtcatggaa atcgtctcta cctgtcggtc
     3601 atgtcgccag cgcggcgtgc ggcagtccgg gcagcggcag cggcagcggc gggacgcaga
     3661 ccggctcgat ggtcagcgtc acaccgaaga gctgccgcac ctggtcgctg atctgccctg
     3721 ccagcgccag cagttgcgcc gcagtggcgc cgccatggtt gaccagcacc agcgcctgtt
     3781 gcggatgcac gccgaccgga ccttgacgca cgcccttcca gcccagccgc tcgatcagcc
     3841 agcccgctgc cagcttgaag tcaccgctgg gcagcggatg gctgaccagg tcgggatggc
     3901 tgcgccgcaa ccgtgcatga tgggcggcac tgacgacagg attgtgaaag aagctgccgg
     3961 cattgggcag ttgccgggga tcgggcagct tgctggcacg aatcgccagc accgcccggc
     4021 gcaagtcagc aacgctcggt gcggtgatgc cgcgcgcccg cagttcgctc tgcaccgcgc
     4081 catggtcgct gcgtggcgac atggcacggt cgagccgcag tgtcacctcg acgatgatcc
     4141 actggccatt gcgcttgaac aggctgtcgc ggtagccgaa ttggcactga tcgcgctcga
     4201 agcggtgcac cgcgccgctg gtggcatcga ccgcgatcag atgatcgaag cagtcggcca
     4261 gttcagcccc gtaggcaccg atgttctgga tcggcgcggc gccgaccgtg ccggggatgc
     4321 cgctgaggtt ctcgatgcca tgccagtcgg ctgcgacgac ggtctcgacc agccgatccc
     4381 agaccacgcc ggcgccggca cgcacccaaa gttggtcgcc ctgctgatgc agtcgaattt
     4441 catcgaacgc catcttcagc gtcaggccgg ggtggtcgcc gcgcagcagc aggttgctgc
     4501 caccccccag cagcagcagc ggccagtgcc gttcacgggc gatactcaac agccactgca
     4561 gcgcggcaag gctgtcgacg gtggcgaagt ggcggctggt cgccggcagc gccagggggt
     4621 tgagcggcgt caggtcgacc tgctcgacga tgtcgacctt catccagcca cccgctgccg
     4681 catggcccgc cccaaccgcc tcacttgacc accttcagca tctgaccggt ccgtggctcg
     4741 ccagccgggt agtgctgatt gagcagccgc agttgtgcct ctttcagtcc cggcaggatc
     4801 gactgctgcg ccagcgtggc atagcgggtg ccatcatcgg cgcggatcag ccggatgcgc
     4861 agcgcctggg ccagcgctcg ctcatcgggc gtcagcgcgt ggaagctggc ggcactctgc
     4921 tgcaacttgc cgtcgtacag ctcgaagtcg gccagctcgc ggctggtgcc gagcagaatg
     4981 aaggcctggg ggcccttgta gatgacgctg acccgcgccg gccgtttgcc ctggcgggtc
     5041 gagaccaccg tgcggccggt caccccctgc aatggctcca ctgccagcgg ctgcacatcg
     5101 ctcagctgca gccgcagccg ctcggcgatg aactgctcgg gcggcatggc gcggttgaga
     5161 tcctgcaccg tcatcacctg ggtcgcctcg ccagaggggg ccactgccac cacccgatcg
     5221 gccaggttct gcacctccca accggtcgga aactcgacgg cgatgcccat gcgggcatgg
     5281 tagaagcggt tctgccgccg caccccctgc tccggactgt cgccatagac cagcccgtcg
     5341 atctgcttca gaaaggactc gacgccgcgg tcggccgccg gccggtcgac gtagagcggc
     5401 agcgcttcat tgaccacctc cttcagccgg gtgtcgttgt cgggatgcga ggcgaacagg
     5461 ccatggtagc cgccgaccga ttgagggtcg cgcccctcgg cctgggcgag ctgcctgcca
     5521 tactcctcct gtgccttcag cacccgcagc acatcgagca tcgcctgcgg cggatagccg
     5581 ctgcgggcca gatactccgc gccgagccgg tcggactcca gctcctgctc gcggccatag
     5641 ccactcagcg tggcctgggc gaggatgttg aacatgtcct gtgccgccga ggagcgcagt
     5701 tcaggcagca gaatctgcgc cacggtgtag ccgatgccgg ccgcctgtgc cgcactctgc
     5761 tgcctgaccg catggcgggc ggtgacgtga ccgatctcgt ggccgagcac ggcggccagt
     5821 tcggcctcgg agtcgaggta ggcgagcagg ccacgggtga tgtagatgta gccgcccggc
     5881 agtgcgaagg cgttgatggt cggcgagtcg acgacggtga aacggtagac cagcccgggg
     5941 cggtggctca tcgccgcaac cttgccgccc acctgctgca cgtactcctg cagcgcctca
     6001 ttgtcgtagt agccatactc cttgaggatt ctcggatggt actgccggcc aatggcgacc
     6061 tcctcggcct cgctcatcat cacgaagtcg gactgtccgg agaccggatt ggtggcacag
     6121 ccgctcgatc cgcccagcag gacccacagc agcagccacc acgcccgcca gggccgggcg
     6181 gcggggcggg tcagtggctc acaggtcggc atagaggctc tcttcacccg gcggccgggt
     6241 cttgaagcgg cggtgcaccc agaggtactg ctgcggatgg tcacggatga tctcttcata
     6301 gaaccggttc atccgcaggg tgtcggcaaa gtcgtcacca ctgggaaagt catcgaacgg
     6361 cgggtagacc ttgatcgaga ggccgcggcc atcggcatcc cgcagaaagg tgagcggaaa
     6421 gaccggaaaa ccggtctgac gcgcatagtc cgagacattg gggatggtgg cggtggcgat
     6481 gccgaagaag ggcacgaaca ggctgcggcg tggaccgaaa tcctgatcgg gcaccaccgc
     6541 caccagcttg ccgctgtggg cgcggtcgag cagctcctgc acctgcttgt gcggaatgat
     6601 gtagacatgg tgccgctcgc ggatccgctt gctcaccgcc tcgaacacgc ggttgccctg
     6661 aatcttgtag agcgcatgga agtcgccatg ccggccgacg atgcgggcgg tcaggtcgat
     6721 cgccaggaag tgaaaggaga tcagcagggc gccctctccc cgctgctgcc gctcgatcaa
     6781 ctgctccagc ccctcgaccc ggctgatccg gtcgatccgc cggtcactgg cccaccaggc
     6841 gaagccggtc tccatcagcg tgatgcccat ctgctcgaaa ttgcgcttca gcagccgttc
     6901 gcgctgcgcc ggcgacagct cgggaaagca gagcgccaga ttgcgccgcc ccaccgccgc
     6961 ccgcgatcgg cccagtgcga acagcagcca gccgatcggt cggcccagtt gatagagcca
     7021 gcggtagggc agaaaggagg agaggtacca cagcccgatc accagccagc tcggccagtg
     7081 ccgcggatgg tagaggctgc cgcgctcttt tgatttggcc atggtcaggc atccggtcag
     7141 ggcagaagac aggcgaccat tctagcgcaa gccccgatcg cggatggagt cccagcaaaa
     7201 aagggcatgg atcactccat gcccttttca tgttcgacac gccagccgct ggcgcgcctg
     7261 ctgcgcctca ggcggccacc agcccctgca gtgccgcggc atcggcccgc acggcttcgg
     7321 gtccacccag aatctggcgg ttgtagccga tccgtttgaa ccagtagtgc acccccatca
     7381 ggtcggtgaa gcccatgcca ccatggccga gggtcgcctt gttggcgatc tcggtgcaga
     7441 cctcggccag gtgcgccttg gcgtggcagg ccatcacgct ggcctcgttg ggaatctcgc
     7501 tctggcagtg cgccgcgtac cagaccagcg agcgcgaggg ttcgagctcg gccgccatct
     7561 cggcacacat gtgcttgtag ccctggaacg agccgatcgg ccggttgaac tgcttgcgtt
     7621 cgagcgaata gaccacggtc ttctcgatca tgcgctgcgc cgcaccgagg ttgtcggccg
     7681 ccagggccac ccggccggcg tcgatcgtct tgcggatcgc cgtggcggca ccgccgacgc
     7741 tgccgagcgg aatggcagtg gcgccatcga acagcagctc ggccagaccg cgggtgcggt
     7801 cgatgttgtt gagcgcattg cgggtgacac ccgcttcatt cgccggcacc agcaccaggg
     7861 tatcgcgccc caccgccagc agcagcagat cggccaccat ggcatcgagc acgaacagtg
     7921 ccttgccatg gagcttgccc tctttcagcg tgagtcccgc cccttcacgg gcacccaccg
     7981 cctcgctcag ccccacgccg atcttcacct cgccggcggc gatcttcggc agccacacct
     8041 gcttctgcat ttcggtggcg gcatgcagaa aggcgaccgg cgccatcagc gccgtgctgc
     8101 tgtagggggc cggggtgacc gcccagccga ggtgctcctg caccagcgca gcgtcgaaga
     8161 agctgagtcc cgcaccgcca tactcctcgg gcacgatgat gccgagcacg ccgaggtcgg
     8221 cgagcccctg ccagattgcc tgatcgaacc cgctgcgctc ggctgcgatc tgacgcaccc
     8281 ggtcgagtgg gacctgttgc tcgacgaagc gacggacggt ttcgctgagc agtttctgtt
     8341 ccgatgacag accaaattcc atcttgttgt tccccctgaa tcaggcgctg accgctttgg
     8401 gctcgcgcgg catgccgagt ccacgttcgc tgatgatgtt cttttgaatg tgcgaggtgc
     8461 cgccgccgat gatcaggccg aggctgaaca tgtagcggct ctgccacatg cccattgcac
     8521 gcaggtattt gctctggtcg tagagaatgc ccagctcgcc gagggcatcg atcgccagtg
     8581 cgcagatgtc ctggttgagg tgggtgccga tcagcttgac caccaggatc ggcatcagct
     8641 ccagctcgtt cttgagggtg ttggtcagca gccgcatgct gtgccactgc atcgaggcca
     8701 cccgcgcctg caaccgcatc agccgatcgc ggtagagcgg gctgtcgatc agccgcacgc
     8761 catcgacggt ctcgctggtc agcagctcgg tcacctggtg caacaggttg gcgctctgcg
     8821 acgggtcacc cagcatgccg cgctcatgct tcagcgtggc attggccacc atccagccat
     8881 cgccacgcct ggcgacgatc tggtcggtct tcagacgcac gtcggtgaag aagacctcgt
     8941 tgaaggtggc atcgccggtc atggtgacca gcgggcgcac ctcgatgccc ggtgtcgtca
     9001 tcggcagcag caggtagctg atgcccatgt gcttgggcgc gtcggcctcg gtgcgcacca
     9061 gcacgaacat catgtcggag aagtgggccg agctggtcca gatcttctgg ccgttgatga
     9121 cgaactcatc gccctcgaca tgggcgcggg tttgcaggct ggccaggtcg ctgccggcat
     9181 tcggctccga atagccctga caccagaaga tctcgccggt gatggtcggc ctgatccagc
     9241 gctgcttctg ctcttcggta ccgagttcga gcagcgttgg aaccagcatc gagatgccct
     9301 ggccactcaa ccccttggag acgccggcct ggtcgaactc ctggtcgacg atgacgctct
     9361 tgagcacatc gggctcgccg ccatagccgc catactgtct ggggatggtg cgggccgcat
     9421 agccctgctc gatcagcagt gcctgccact cgcgcgcctg ggcagtgggt cgccccatct
     9481 cgccctgcag gctgggcgac ttgtggccat gcttggccag aaaggcgcgc acctcctgcc
     9541 gaaaatcttc gtactctttg ccgtacctga gatccatgga tgactcctcg cggctccctg
     9601 cccagtctgg gtgctgccga ctcgcgccgg cggacgctga cagggagaaa aaagtgttgt
     9661 tatggtggca acggctgaac caccgtcgcg cagccaagct agtgccaacg cccggcaaat
     9721 gcaagcgccg gggtcacttt tcggaccgat ccgcgtcgtc gccgcgctgc caggcaacgc
     9781 cgcgggcata gagccagtcg cgctcgagcc cggtcatgcg gctggccaga cgcgccgccc
     9841 gcgccagtgg cagttcagtc agcagttcgc gcagcatccg ctcggcctgc tgctgcgaca
     9901 gtgtcacctc gcgctcgcaa ccggccacga cgatcaccac ctcgccgcgg gccgactcct
     9961 cgccgccacg gaagcgctcg gccagttgcg ccagcgtggc gcgccgcacc gactcatgca
    10021 gcttggtcag ttcacgtgcc accaccgccg accgttcggc accgaaagcc gccaccatcg
    10081 cctcgagggt ggcgaccaga cggtgcggtg cctcataaaa gagcagcgtc cgactttcgt
    10141 tggccagccg ctgcaactgc gcactgcgcg cctcttcacg ggacggcaga aaaccttcga
    10201 aggcgaagcg atcgctgggc agccccgcca ccgacagtgc ggcaatggcg gcattggcac
    10261 cgggaatcgg aatcacccgc ccaccagcct ccgccacccg cttcaccagc cggtaaccgg
    10321 ggtcggagat ggtcggcgtg ccggcgtcgc tgatcaacgc cacccgctcg ccggcgagaa
    10381 tgcgcgccag cagccgctcc ctgacccgct ccgaactgtg ttcgtggtag gcggtcaccg
    10441 gggtggaaat gccgaaatgg cttagaaggc cccggctatg tctggtatcc tcggcagcga
    10501 tcagatcgac accacgcagc acctcgagcg cacgcagggt gatgtcgccc atgttgccga
    10561 tcggtgtcgc cacgatgtag agcgccggcg ccaacagacc ttcgaaactg accgcggttg
    10621 tcccactcac tcaagcatca ccctctgttg ccgactccca tgccctgttc aactctccag
    10681 tttcgatccg cgcgccccgg tgcctggctg ctcgccctgc tgtggctgtt gaccgccgcc
    10741 ggctgcgtct ccacccggcc ggtcgagccg ccgcccggca cgcccgagca gctcaccgtt
    10801 gaagcgctgc tgaaacaggc tgaaaagctc tcttctccgc agcgcgaaga atacctgatc
    10861 ctggccgccg aacagcgtct gcaacagcag cagatcgccg aagccgagca gctcagcagc
    10921 ccgctcgaca tcgggctgct gccgctcgac ggccgccggc gtctggcact gctgcgggcc
    10981 gagatcgcgc tcgacggcaa tcagctcgat accgcttcca actggttcaa cctgccctcg
    11041 gcccagttcg aacaactgag cgatgcatcc accctcgccc ggctttcgct gctgcgcgca
    11101 cgctggttcg aagccaagga tcagcctttg ctcgccgcgc gtgaacgcat cctggccgac
    11161 ccgctgctgg ccgaaaccgc ccgtggtgac aaccatcggc agatctggac gctgctcgga
    11221 cgggtcaatc ccgatcgcct gcgcgccacc gcgcgggcac tgccggacaa cgacctgcgc
    11281 ggctggctgg agctggccgc gctcgaccgg cagttcaccg aagccaaccc gcaacggctg
    11341 gccgccctgg ccgactggcg cagccgctgg ccccgccatc cggccgccat cgaaccgccc
    11401 gccgggatcg ccgacacact gcggccgctg gaggccgcgc agcagcaagg tcacgctcgc
    11461 gtgctgctgc tgctgccgct gtccggaccg cttgccgccg ccggcgaggt ggtgcgcgat
    11521 ggcttcatgg cccgctacta ccagcagctc gctgcccagc aacccttgcc ggaactggtg
    11581 ctgatcgaca gcgccgccac gccccaggcc agctggttgc cccaggcgca agcgctcaag
    11641 gtcgatgcga tcatcggccc gctgccgaag gagatggtcg aggccctcaa ccagcagccg
    11701 ctcgacattc cggtgctggc actgaactac ctgcccgacg gagtgacacc acagcccaat
    11761 ctccatcagt tcggtctggc caacgaagtc agtgcgcaac tgacggcccg gcaggccagc
    11821 agcgccggcc aacgccgcgc cgcgctgctg tttgccgaca gcccctggag ccggcggctg
    11881 gagcgcgcct ttcgcaccga gtggcacaag ctgggcggcg aagtggtcgc cagcgccagc
    11941 ttcaacgagg ccacggccga tcatgcgcag gcggtccggc agttgttgca ggtcacggca
    12001 cctgctgccc cgactgtcgc cggcagcgcg accgctccgg ttgatggcac cgccgccacc
    12061 gccagccgtc gacaggacat cgactgcatc gtcctgatcg ccgcgccgga acagggtcgg
    12121 ctgatcgcgc cgctgttccg gctctacttc gccggcgacc tgccgctcta cggcgtcacc
    12181 acgctctacg cgggccgcag caacccacgg caggacagcg cgctcgacgg catcatcttc
    12241 aatgacattc cctggctggt cgacccggcc tttaccgaac gggccgaaat cgccgccctc
    12301 tggccggccg cctatccgga ccatgcgcgg ctctatgcgc tcggccgcga tgccctcgac
    12361 ctgctcccgg cgctcgaaca gctcaaggcg ccaggcgccg aactgcatgg tgccaccggc
    12421 ctgctgcggc tcgatgccga tcgccgcatc cagcgcgaac aggttccgac ccggttcgaa
    12481 aatggtgtcg tggtcagact gcccgcagcc gggagcggac cagcacgatg aagctgttcg
    12541 accggcaacg caaccggcgc atcggcgccg aggccgaggc cagtgcgctc gaccatctgt
    12601 cacgggccgg attgcggctg gtggcccgca actaccgctg ccgtggtggc gagatcgatc
    12661 tgatcatgcg tgatcggcag acactggtgt tcatcgaggt gcgttaccgc tcaggcggcc
    12721 gcttcgccag cgccgccgag agtgtcgatg ccagcaaaca gcggcgcatc accaccgccg
    12781 cgcaccacta tctgcaacga cagccgcaat accgcgacct gccctgccgc ttcgacgtcg
    12841 tcgccttcga tcccgccgcg cagcagggtc cagcgctaca accccgctgg attcaggatg
    12901 cctttcactc gacccactga accatgaaca cgcaacaacg catcttcgac ctgttccagg
    12961 agagcatcgc caccaagcag gccgcactgg agctgctcgg ccccagcatc gaactggccg
    13021 cacagatgat ggtctcctgc ctgctgcagg gcggcaagat tctctcctgc ggcaacggcg
    13081 gctccgccgg cgacgcccag cacttctcgt cggagctgct caaccgtttc gagcgcgaac
    13141 gacccggtct gccagccatc gccctgacca ccgacagctc gaccctgacc gccatcgcca
    13201 acgactacag ctaccacgag gtgttcagca agcaggtgcg tgcgctgggt caggccggcg
    13261 acctgctgct ggcgatcacc accagcggca actcacccaa tctgctgcag gccgtcggcg
    13321 cggcccatga ccgtggcatg caggtggtgg cgctgctcgg caagggcggc ggtgaactgc
    13381 gcaatctgct gggagaggtg gatgtcgaga tctgcgtccc ctccgaggtg acggcacgca
    13441 tccaggaggt gcatctgctg gtcatccact gcctgtgcga cctgatcgac aatcagctgt
    13501 tcggcagcat cgagatgtga cgaatcgcgc gccgcaacag cgcgcacgca cccctacttc
    13561 accaccgaaa gatggcgcgg tgacgacttg ccagccgtgc ggtccggacc gccgggcggc
    13621 ggctcctcat cattgggatc gaagatcagc ccctgaccat tctccttggc atagatgccg
    13681 cgcacggcat gcagcggcag ggtcaggtgg gtcgacacgc caccgaaccg gcaatcgaaa
    13741 tcgatctcgc tgttgccgat gatcagcgca ttcaccgctc ccggcgccac attgagcacg
    13801 attctgccct cgttgacata ctgcctcggc acctgcaccc ccggcgcgtc ggcgtcgatc
    13861 accagatagg gcgtgcagcc gttgtcgagg atccactggt ggatcgctcg caacagatag
    13921 gggcgctgtg aactcatcga cggcatgact gcaccctgaa agattggccg aaacaggcgc
    13981 ggtcagcgca tctcccgttc ctgatcggtc agactggcct gaaaggattc acgctcgaac
    14041 atccgctcca tgtacttggc catcggcttg atctgccgga tcggcagttc gatgcccatc
    14101 aacggcaggc gccacagcag cgggacgacg caacagtcga ccagcgagaa ctcctcactc
    14161 atgaagaagg gcgcctcttc gaagatcgcc gccacgccga ccaggctgtc acgcagctcc
    14221 ttgcgcagct tgttgagggt cgcctccttc tcctcgccac cgaggatccg gtcggccagc
    14281 ggcgaccagt cgcgctcgat gcggtagatc agcaggcggc tctgcgcccg cgcgaccgga
    14341 tagaccggca gcagtggcgg atggggaaaa cgttcatcca gatactccat catgatgttg
    14401 ggctgataga gcaccagctc ccggtcgacc agcgtcggca gcgtgttgta ggggttgatc
    14461 tcgaccagat cggtcggctt ggtctcttcg gtcagttcga tcagatcgac agtgaccccc
    14521 ttctcggcca gaacgatgcg cacccggtgg ctgtagtggt ccgtggcgcc agacagaaac
    14581 gtcatggatg aacgcttggt gatcattcca atgcgcaatt cagcacgttg cgatttcaaa
    14641 gccaatacct cgatggatga cgaagatcag gttgttatcc gtaccatgca gaaatgttct
    14701 cgcggccaag gccgcaaccc cgcgcagaaa cagaaaaggg cgggcgattg gattcgcccg
    14761 ccccaggacg gaaggagacg cgtctcagtg gacgtctttc cagtactcgc ggttcagcat
    14821 gtaggccacc acgaagaaga aggcgaggaa cagcagcacg tagacgccaa gccgatggcg
    14881 ctcgagcttt tcgggctctg cgacataggc caggaagttg accagatcgc tgaccgccgt
    14941 gtcgaactgt ttggccgaga gcttgccgct gccttcgacc acctgcagtt gatgacaatc
    15001 ctcaacggtg atgttctggc cgctcagcgg gtcacgcttg atgccgccgt tttcggcaag
    15061 ctgcggcacc tgcttgcaga ccatgcgctg ctcgccctga agctcccgca gcacattggg
    15121 cataccgaca tccttgaaca ccacgttgtt gacaccccat ggccgggccg gatcggtgta
    15181 gaaggcacgc agatagctgt agacccagtc gctgccacgc aggcgcgtga ccagcgtcag
    15241 atcgggtggc gttgcaccga accagcgctt ggcattggcc ggcggcattg cattcgtcat
    15301 cagatcgccg atcttggcct gcccgaacac cagatgcttc atgaacagtt caggcggcac
    15361 gcccagcccc agcgacagct tttcataacg ctcgtacttg agcgaatgac agcccatgca
    15421 gtagttcatg tagagggccg caccgcgctg cagggcatgc ctgtcggcgg ggtcgatctt
    15481 gacatggtcc agcgcgacct cttcgcccga ggcccacgcc agcagtggca accagccgag
    15541 gatgagtatt ttcagaattt ttttcatcag cctgtcactc tctgcggaac cggcttggtc
    15601 ttctcgagcc gggtgtaaaa gggcatcagg aaaaagtagg cgaaatagag cacggtgcag
    15661 acctgcgcga gcagggtccg ctgtggcgtc ggcgccatcg cgcccagcac gcccaagatc
    15721 aggaagctga ttccgaacac ccacaggaag agtttgctca acaacccctt gtagcgaatc
    15781 gatttgaccg gactgcgatc gagccagggc agcacgaaca ggatcgcaat cgcaccggcc
    15841 atcaccacca gcccgccgaa cttgtcgggc accgcccgca gcatcgcgta gaagggggtg
    15901 tagtaccaga ccggcgcgat gtgtgccggg gtcttcagcg gatcagccgg ctcgaagttg
    15961 gcaaactcga gaaagtagcc acccatctcc ggaaagaaga agatcaccga acagaacacc
    16021 gccagaaaga ccacgacccc gacgatgtca tgcaccgtgt agtaggggtg gaaggcgatg
    16081 ccatcgagcg gaatgccgtc cttgtccttc agcttcttga tctcgacgcc atcggggttg
    16141 ttggaaccga cttcgtgcag cgccagcagg tgcagcacca ccagcgccag cagcacgatc
    16201 ggcagtgcca ccacatgcag cgcaaagaag cggttcagcg tgatgccgga gatcaggtag
    16261 tcgccgcgca cccactgcgc cagcggctcg ccgatgtagg ggatggcggt gaacagggag
    16321 acgatcacct gcgcacccca gaacgacatg ttgccccatg gcagcaggta accgaagaag
    16381 ccctcggcca tcagcgccag atagatcatc atgccgaaga tccagatcag ttcgcgcggc
    16441 ttcttgtagg agccgtagag caggccacgg aacatgtgca ggtagatcac cacgaagaag
    16501 gccgaagcac cggtcgaatg catgtagcgc agcaaccagc cccactcgac atcgcgcatg
    16561 atgtattcga ccgaggcaaa ggcgccttcg gcactggggt tgtagctcat cgtcaaccag
    16621 atgccggtca gcagctggtt gaccagcacc agcatcgcca gcacgccgaa gaagtaccag
    16681 aagttgaagt tcttcggcgc atagtacttg ctcatgtgcc tttcataggc ttcgacgatc
    16741 ggcagacgct cgtccaccca gccgatcagc ccgctgatcc agttgctcat caggcagccc
    16801 cctcttccga accgataaga atcactttat caccgacgaa actgtagggc ggcacttcca
    16861 gattggtggg tgccggcacc cccttgtaga cccggcccga caggtcgaag cgcgagccgt
    16921 gacaggggca gaagaagcca cctttccagt cagcgccgag atcggccggc gccagttccg
    16981 gacgaaactt gggcgagcag cccaggtggg tgcagacgcc gagcaccacc aggtactcag
    17041 gctcgatcga acgatagggc gacttgatcg actctggctg ctgggaggtg accgcgcctg
    17101 ccgggtcggc cagcacggca tccatcttcg tcagctggtc cagcgcctcc ttggtgcgac
    17161 gcacgacgaa caccggtttg ccgcgccact cgaccaccac catctggccg ggctcgagct
    17221 tgctgagatc ggccttgacc ggcgctcccg ccgccttggc tttcgcgctc gggttccagg
    17281 agccgacaaa gggcaccgcg gcaccaacca cccccacggc acccaccacc gaggtggcgc
    17341 cgatcaggaa tcgccgcctg ccggcattga cctcgccttc actgctcatg agtttttcac
    17401 tcctcgatca atatgttctt cttgacggat actggatgcg attggacgcc cagtgaaggg
    17461 tcggcagccg ctgaaaagcc accgaaaaat gcgccgatcc accgcagatg acacgcgcaa
    17521 aaccggcgaa attctaatga tttccgcttt gcgtgacaag ccggaccact gcctgccgtt
    17581 gttcatggaa ccaaaaaaaa acgcccagaa cacaggaact gggcgttttg caatgttcga
    17641 aaacggaacc cctgcgctgc gctgcccaga caggccctgt caacgcttcg agtattgtgg
    17701 ccgcttgcgc gccttgcgca gaccgatctt cttgcgttcg acctcgcgcg catcgcgggt
    17761 cagatggccc tctttgcgca gggtgccacg cagatcttcg ttgtactgga ccagcgcacg
    17821 ggcaatgcca tgacgaatcg caccggcctg accaaagctg ccgccaccgc tgacgctgat
    17881 tttggcatcg aaccggccgg ccagattggc cgcttcgagc ggttgcatca ccaccatgcg
    17941 cgccacttcc cgaccaaaat actggtcaag cggccgatcg ttgaccgtga tggtgccgct
    18001 gcccgcccga aggtaaaccc gcgcggcagc cgtctttctg cgcccggtgc cataatagtg
    18061 tgtttgcgcc atagcctgaa tcgatcctga tctcgttctg aaagatgtta caaatcaacc
    18121 ggaagcggtt gttgcgccgc atgcggatgc tcggagcccg catagatttt caactttttg
    18181 aacatcgcct gacccagcga gttcttgggc agcatgccct tgaccgctgc accgaggaca
    18241 cgctcaggat ggctgccgat catctcttcg aaagagctgc tcttgagccc gcccggatat
    18301 cccgtgtgac ggtagtaggt cttgtcggtg cgcttgctgc cagtgacccg aatcttctcg
    18361 gcattgatca cgacgatgta gtcgccggcg tcgacatgcg gggtgaagat cggcttgtgc
    18421 ttgccccaca gacgctgggc gatttcactg gcgagccgac ccaaggtctt gccgtcggca
    18481 tcgaccagca gccactcccg ccggacttcg gccggttttg cgcttagagt cttcataaaa
    18541 aactgttgcc tgataccctg atccaaaatg aggcggcatc ttaccgatgc ggcaacacga
    18601 tttcaacctt cttgcacccc gacaccgcaa tcgaagctgg cccagaccgg gcagtggtcg
    18661 gagggccgct ccatgccgcg gatgtcgtag tcgatgccga cctgctgcag ccgatcgatc
    18721 atgccgggcg tggccagcag cagatcgatc cgcagaccgc gtcgcggctg ctgttcgaag
    18781 ccccgactgc ggtagtcgaa ccagctgtag aggtcatcgc ggtcgggatg aagcacgcgg
    18841 tagctgtcct gcagccccca gaccagcagc tcggcgaacc aggcgcgctc ctccggcaga
    18901 aaactgcatt tgccgctgcg cagccatctt ttgcggttct cttcaccaat gccgatgtcg
    18961 atgtcggccg gcgagacgtt gaggtcaccg gtgacgatca atcgccgcgc cggatcatag
    19021 tcggcatcga gcagttgtct caggtcacgg tagaacttct gtttggccgg aaacttgacc
    19081 ggatggtcgc ggctctcacc ctgcgggaag tagccattga tgaccgacag ttcgccgcca
    19141 tcggccaggg gatagtcagc ccggatcagc cggcactgtg cctcggcgcc gtcaccagaa
    19201 aagccgcatt cgacctggcg cggcgcaacc cggctcagca gtgcgacgcc atagtggcct
    19261 ttctggccat ggtagacgac atggtagccc agcgcgtcga tggccgcctg cgggaactcg
    19321 gcgtcgctca ccttggtctc ttgcagtgcg atgatgtccg gccgatgccg ctcgatcact
    19381 gctgcgagct gatgcagccg ggcacgcaat ccgttgatgt tgaaactgat aactctcatg
    19441 acccgctcca cgccccgtct cctctggaac agcggattat aatggcgggt cgagatccat
    19501 tcatgttcgc agtgccaagg agatgttcat gcaagcgccg atgcattggt tcaccaccct
    19561 tctggacctt ctggcggtgc tgttcatttt catgctgggt gcgctctgcc tgacgctgct
    19621 ggcctggtat ttcatcgaca gacatcagaa aagccacgcc atccgccgca actttccgtt
    19681 gatcggccgc tttcgctatc tgttcgagtg gctgggagag ttcttccggc aatacttctt
    19741 cgccatggac cgcgaggagc tgcccttcaa tcgcgccgag cgcagttggg tctaccgcgc
    19801 cgccaagaac ctcgaacaga ccgccgcctt tggttcgacc aacaacctgg ccacgcccgg
    19861 cgcactcttc ttcgttcaca gcaccttccc gaccgccgcc gcggaaccgc cggcgccggt
    19921 ggtgatcgga cccgatacgc cccaccccta tcaggccgac aagttcttca acatctcggc
    19981 gatgagttac ggcgccatct ccagacccgc tgtgctggca ctgtcacgcg gtgcggcgct
    20041 ggccggctgc tggctcaaca ctggcgaggg cggattgtcg ccctatcacc tcgaaggcgg
    20101 tgccgacctg gtgttccagc tcggcaccgc caagtacggc gcgcgcgacc cgcagggcaa
    20161 tctgagtgaa gagaagctgg cacggatcgc cgcccatccg gcggtgcggc tgttcgagat
    20221 caagctgagc cagggggcca agccgggcaa gggcggcatc ctgccggccg ccaaggtgac
    20281 cgaggagatc gccgcgattc gcggcatccc gatgggccac gactcattga gtccggctgg
    20341 ccacctcgac gcccgggaca tcgacggact gctgacgctg atcgaccggg tcagacgggc
    20401 caccggcaag ccggtcggca tcaaggcctg catcggcgag agcagttggc tcgatcagct
    20461 ctgcatcgag ctcaactgcc gcggtttcga cagcgcgccc gatttcatca ccgtcgatgg
    20521 cgccgaaggg ggcagcggtg ccgcgccaca gagcctgctc gactacatgg gactgccgat
    20581 ccgcgagagc ctgccgctgg tggtcgacac gctggtcacc catggcctgc gcgagcgcat
    20641 caaggtggtg gcctcgggca agctgatcaa cccggtcgag gtggcttggg cgctctgcat
    20701 gggcgccgac tgcatcaatt cggcacgcgg cttcatgttc gcactgggct gcatccagtc
    20761 gctgaagtgc aaccagaaca actgcccgac cggcatcacc acccaccgca tcgacctgca
    20821 gcaggggctc gaccccaccg acaaggccga acgggtgcgc cactaccagc agaatctgga
    20881 acgggaggtg gcggtgatcg cccacgcctg cggcgtcgag tcgccccggc agttgcgccg
    20941 ccaccatgcc cgcatggtgg tcgaggccgg gcgctcggtt tcgctgcatg agcgctaccc
    21001 gccggcagtc atccctgtga cagcagttgg cgcagatcga tgatggcggc gtgggcgcga
    21061 ctgatatagg aggccagcac cagtgaatgg ttggcgaaca ggccaaagcc gtcaccgttg
    21121 agcaccatcg ggctggtgag cggtcgctgg gtcgcttcga gctcgcggat gatctgccgc
    21181 aggctcacct gggccccctt cttctgcagc acctcggcga aatcgacctc gaccgccttc
    21241 aggatgtgca gcagcgccca ggccgagccc tgcgactcgt agtagacgtc atcgatctgc
    21301 aaccagggcg tgcgggtcat ctgctcgact gccgccggcg ctgcggcggt cgcatcgacc
    21361 gtgccgcccg aagtcgtgtc aatccgcacc tggccgacac tggcgctcag ccgctgcgac
    21421 aggctgccga ggcgggtttc gaccgtcgcc agccagaagt tgaggttgtc ggcccgggca
    21481 tggaactgcc catcgttgtg attgtcgtcg ctgctgcgct gcaggtagct gcgcagtgcc
    21541 ctgagcccct ggcgatactc gccctcggtc gatggcagcg cccaactggc gttgtcgaac
    21601 ttgaactgcg gttcggcgat gaccaggtcg cggtcctcgg ccgactgtga ctgcgagcgg
    21661 ctgaaggatt cacgcatcgc ctcggtcagg tcgcgcaact gggtcagcgc cccgaactcc
    21721 cagttggcga tgtcatcgag ccagacgccg ggcggaaaga ggtcattgcc gaggtaaccg
    21781 cccggcttgt cgagcaaccg tgtgccgatg ccgatcgcgg tggccgtcac cacggtgccg
    21841 ggcggcggcg tggtcgggcc acccagttgc gccagctgcg tcgccgcctc ggcgcgcacg
    21901 tcgaacagtg ccggcttgcg actccagtag aacgacagcc cggtccacac cagcagcaac
    21961 agaccgatcg gcagtgcgat cagcgtgcgc cgcagcggac tggaacggtt gctttcgcca
    22021 ctctgcattg tgcactctcc tttactgccc gcagacaccg aaccgcatgg tcggcgatgc
    22081 aaccatgctt ggcaagacaa cccctccccg gcacgcaata tcggcagtga cgctgccgcc
    22141 gtataatcgg tccttttcgt ccgcaataca gggctgcgca tggataagat cgtcgttcgc
    22201 ggggcacgca cgcacaatct gaagaacatc gacctcgaac tgccgcgcga ccagctcatc
    22261 gtcatcaccg gactctccgg ttcgggcaaa tcctcgctgg ccttcgacac cctctatgcc
    22321 gagggacagc ggcgctatgt cgaatcgctg tcggcctatg cccggcagtt tctgtcgatg
    22381 atggagaagc ccgacgtcga ccacatcgaa gggctgtcgc cggcgatctc gatcgagcaa
    22441 aaggccacct cgcacaaccc gcgctcgacg gtcggcaccg tcaccgaaat ccatgactac
    22501 ctgcgcctgc tcttcgcgcg ggtcggcgaa ccgcgctgcc cccggcacca ggcgccgctc
    22561 aatgcccaga ccgtcagcca gatggtcgat caggtgctgg cgctcgaccc cgaacggcgc
    22621 ttgattctgc tggcgccggt ggtgcaggag cgcaagggcg agcatctgca gctgctggag
    22681 acgctgaagg ccaagggctt catccgcgcc cgcatcaacg gcagcgtggt cgagctcgac
    22741 gaagcgccac tgctcgagaa aaaccgcaag cacagcatcg aggtggtggt cgaccgcttc
    22801 cggccacgca gcgacctgca actgcggctg gccgagtcgt tcgagaccgc gttgcagttg
    22861 agcgacggca tcgtcaaggt gctgctcgat gatggcgaag agctgatctt ctcggcccgc
    22921 ttcgcctgcc cccactgcgg ctacagcatc aaggagctgg aaccgcggct gttctcgttc
    22981 aacaaccccg ccggtgcctg cagcagctgc gatgggctcg gagtgaagca gtcgttcgac
    23041 ctccagcgca ttgtgcccaa ccccgagctg agcctgagcg agggggccat tcgcggctgg
    23101 gaccgccgca atgtctacta ctttcacctg ctcagttcgc tggccgacca ctgcggcttc
    23161 agcctcgacc tgccctttgc caagctgaag aagcgccatc aggagatcat cctgcatggc
    23221 agcggcgacg agcagatcga cttcagatac atgaatgacc gtggcgacct gttcaagcgg
    23281 cgtcacacct tcgagggcat cctgccgaac atggagcggc gctaccgcga aagcgactcg
    23341 ctgtcggtgc gcgaagagct ggccaaatac ctcagcagcc acccctgcgg cgactgcggc
    23401 ggtgcccggc tgcgtgagga ggcacgccat gtcttcatcc atgactggaa cctgccggcg
    23461 gtgagccgcc tgccggtggc cgaggcgctg cacctcttcg acaccctgac cctgcagggg
    23521 cagcgcgccg agatcgcgag caagatcatc aaggagatcg gcgcccgcct gcgctttctg
    23581 accgatgtcg gcctcggcta cctgagcctc gaccgcggcg ccgagaccct ctccggcggc
    23641 gaggcgcagc gcatccgcct ggccagccag atcggcgccg gcctggtcgg agtgatgtac
    23701 atcctcgacg aaccctcgat cgggctgcac cagcgcgaca acgaacggct gctgcgcagc
    23761 ctgttccacc tgcgcgacat cggcaacacc gtcatcgtgg tcgagcatga cgaggatgcg
    23821 atccggcagg ccgaccacct gatcgacatc ggccccggcg ccggcctgca tggcggccag
    23881 gtggtcgccc agggcagcgt ggccgagatc atcgccaatc cggcgtcgat caccggcgac
    23941 tatctggccg gacggcggca catcgcggtg ccggcacggc gggtggcgcc ccaggcggag
    24001 cgctggatca ggctgcgcaa cgcccgtggc aacaacctgc gcggcatcga tgtcgccatt
    24061 ccgctgggcg tgatgacctg cgtcaccggc gtctccggtt ccggcaagtc gacgctgatc
    24121 aacaacaccc tctacccgct cgccgccacc cggctcaatg gcgccggccc gctgccgatc
    24181 gccccctgcg acggcatcga cggactggaa ctgctggaca aggtgatcga catcgaccag
    24241 agcccgatcg gccgcacgcc acgctccaac ccggccacct acaccggcct gttcaccccg
    24301 atccgcgacc tgttcgccgg cacccaggag gcgcgttcac gcggctatca ggccggacgc
    24361 ttcagcttca acgtcaaggg tggccgctgc gaagcctgtc agggcgacgg cctgatcaag
    24421 gtggagatgc actttctgcc cgatgtctac gtcccctgcg acctctgcca gggcaagcgc
    24481 tacaaccgcg agacgctcga aatcctctac aagggccgga gcatccatca ggtgctggag
    24541 atgaccgtgg aggaggcgcg ggagttcttc gcggcggtgc cgatgctgac gcgcaagctg
    24601 caaaccctgc tcgatgtcgg tctgggttac atccgcctcg gccagtcggc caccaccctc
    24661 tccggcggcg aggcccagcg ggtcaagctg gccaaggagc tgtcgaagcg cgacaccggc
    24721 cgcacgctct acatcctcga cgagcccacc accggcctgc acttccacga catccagcag
    24781 ctgctgcagg tgctgcattc gctgcgcgac gaaggcaaca gcatcgtcgt catcgaacac
    24841 aacctcgacg tgatcaagac cgccgactgg atcatcgacc tcggacccga aggcggcgac
    24901 cagggcggca ccctgatcgc ctgcggcacg ccggagcaga tcgccacaca ccccgactcc
    24961 cacaccggac attttctgcg accgctgctt ggcgagatca gatcggccgc aggctgaccg
    25021 gaatcaccct gccgccagca gcatctcctg aacatcaccc atgaaagaaa aaaagcccga
    25081 acctgtcacc accggttcgg gctgcggaat cggccgcgat cagcccacgc tcagtcgtcg
    25141 ctgccgccca ggccgaacag cgccagcagg ctgacgaaca ggttgtagag ggagacaaac
    25201 aggctggtgg tcgccagcac atagttcgtc tcaccaccgt tcacgatgcg gctggtttca
    25261 tggaggatca tgccactcat cagcagcacg aacatgcccg acaccgccag gctcagggcc
    25321 ggcatctcga agaagagcgc gcccagaccc agaacaaagg ccaccagcac gccgatgaac
    25381 agaaacttgc ccatgaaact gaagtcacgc ttcgacgtca gcgcaatggc cgacagcccg
    25441 aggaaggcga cggcagtggt tcccagcgcg ttggtgacga cggcactgcc atttggcaga
    25501 gacagcacgg aactcagcag cgggcccagc gtataaccca tgaaaccggt cagcgcgaac
    25561 accgacagca ccccccagcc gctgtcggcg gtcctgttga tcagaaagct caggccgaaa
    25621 aagccaatca gcgtcaggag caggcccgga tgcggcaacc ccagacgcat cgacaggccg
    25681 gccaccgcgg cgctgaacgc cagcgtcagg cccagcaggg cataggtgtt gcgcagcaca
    25741 cgtcggctgt cgaccgacaa ggccgtgctt tcacgctcgg cactgcccag cagcagcgcg
    25801 cgggtctggt ggttcggatc ttttgacatg atgcactccc tcaatcgatg atcgaaaaat
    25861 ggttgaagat tcaggtcacc gcgctggcat catccaatgt ctgcaagcca tgacggttgc
    25921 ctccggcccg gtgccgcccc aattgggggg cacgcacggc ccggtcacgg gctttgccaa
    25981 tctggcggcc gatggtctgc accgcacggt gcagcacgcg gtcgaccacg gcaagcaggt
    26041 gatcgccgcg gtcgcgcacc accgcggtgg tgtccaccag ttcgaccacc agatgacact
    26101 ccttgtcgac tccgccacgc gggccattca catcactcag atgcagggtc acgcccatgg
    26161 cgcgctgacc gaagcgcgcc agcagctgcc gcaaacggcc gtggagatgg tcgtgctctt
    26221 cacgggagag cctgatgcct tgggtgcaga tcgacacgtt cattggatgc gcctcggtgc
    26281 aggttggaca tgcattgcaa cctaccggtc aggttttctc acgtaaattc gaatttgatt
    26341 aaaactcaat aaggaaaaat cgaagtgaac taccggcact tgcaatactt ctgggccgtc
    26401 gcccgcctgg gaggggtgat cagggccagt gagcagctgc acgtcacccc ccagacggtc
    26461 agtggccagg tccagttgct cgaagaggcg atcggacgac cactgtttcg caagaagggc
    26521 cgcaagctgg agctgaccga aaccggcgag ctggtctaca gctatgcgcg cgacatcttc
    26581 cacttgggtt ccgagctgga agagctgctg cggcaacccc gcggcgtcca gcgcccggtt
    26641 gaactgcggg tcggcatcgt cgatgccgtg cccaagctgg tggccggcca attgatgaca
    26701 ccggccatgg tgggggcaca gcctttccgg gtcatctgcc gcgagggcaa gatcgagagt
    26761 ctgctggccg acctgtcggt gcaccggctg gagctggtcc tgtcggaccg ccccatgcca
    26821 ccggccctga gcgtgagcgc ctacacccat cgactgggcg gctcgcatgt gggcttctac
    26881 gctgcgaacc agctggcggc gaccttcgac agaccctttc cccaactgct cgacggggca
    26941 ccgctgctgt cgccgggcag cgagtcggca atgggcctgc gtctgcgcca gtggcttgga
    27001 aagcacaaac tgcgtccgcg ggtggtgggc gagttcgacg acagcgcgat ggtcaagtca
    27061 ctgggaatgc agggactggg cgtctttatc gcgccgcggg tgctcgacgc ctcggtgtgt
    27121 cggcaatatg acgcgacctg cctgggcgag gccaccgacc tgatcgagga gttccacgtc
    27181 atcacggtgg agcgccgcat cacccacccc ggcgtggcgg ccatcaccga ggccgcccga
    27241 caggggctgt tcgccgactg ggggcgttga gccaccagcc ctgcggatgc gatgccgcac
    27301 tggcgaagac gcgggcggcg ttatttcgga ctcgcctgcc gctcggcgtc gaggcgctcg
    27361 gtgatcagcg ccgccggcat cggccgggcg aaatggtagc cctgcatgac acggcagccg
    27421 agctcctgca gggtttgcga ctgggcagct ctctccacac cctccgccac cacgccgacg
    27481 ccgaatgcct tggcgaccgc caggatcgcc tgcaccaccg cccggtcacc gtctgcgtcc
    27541 ggcaggccgg cgacgaaggc acggtcgatc ttgagccggt cgaccggcag ctcgcgcagg
    27601 ttggcgagcg aggcgtggcc agcaccgaag tcatcgatgg caaaacgcac gccgagttca
    27661 cgacaggcct gcatcagtgc ctgcgcgcgc ggcaggtctt cgagcgccgc ggtttcggtg
    27721 atctccagca tcagccgctc gcgcggcagg tcgccgtgat cggccagcag ttcggtgagg
    27781 ctggcgagaa aaccctcctc cagaaactcc cgtgccgaca cgttcaccga cacggcgatc
    27841 gggtggccct gccggtgcca cgccgcagcc tgcgccagcg cgctgcgcag cacgtagcgg
    27901 ccgagtgcga ccatcaggcg ccggctgtgg cgctccacat ccgggatgaa gctgaccggt
    27961 ggacgcaggc tgccgtcggg ctgtggccag cgcagcagcg cctcgaccgc caccatgtgg
    28021 ccacccggca gctcgacgat cggctgatag tgcagaacga agcggccgtc gttgagcgcc
    28081 tgctccacct cctccagcag cacttcgcgc tggcgcacgg cctgtgcgat ggagcggttg
    28141 aagaagcaga cctcacgctc gccgtggcgc cgtttggcct cgtacatcgc cagatcggca
    28201 ttggcgagca gcgtgccggc gtcgtcgccg tcttcgggaa aagccgcgat gccaatgctc
    28261 ggcgtcaccg ccgtggtgcc acccgccacc gccacgcgca gcgtcaagcg tgccttgacg
    28321 cgcgccgcca cggtggctgg ctcctccggc caggactgga actccatcag cagtacgaac
    28381 tcgtcgccgc cgaggcgggc gatcacgtcg ccgccacgca ccacctcgcg cagcccctgc
    28441 gccacgtcgc gcagcagggc atcgccgacc gcatgcccgg cccggtcgtt gacggccttg
    28501 aagccgtcga ggtcgatcac catcagtgcg tagcgccgcc cgtagcgtcg accatcggcc
    28561 accgcctgtt gcagacgttc gagaaaatgg cctcggttcg gcagacccgt caggtcatca
    28621 tggttggcgc ggtgctccag acgctgttcg agtcgccagc gatggtgagc aagaccgact
    28681 tgggcggaaa tggtgctcag cagtgacagc agcgcctggt cgggcgcgtg caccgcattc
    28741 ctgaaccagg agaccaccag cacaccgacc gcctcgcccg cgctgtgcag cggcacggca
    28801 agattggcgc gcagcccgct cgccacgaac tccgcaatgg cgcccggatg cgctggatag
    28861 tccggcacga aaaccggacg acggtgcgtc aaagcctcgc cggcaacgcc cgcctgcacc
    28921 ccgaagcgat agccgagaaa cttgtcgctg tacccatccg gcacgccgag aaagaactga
    28981 tattcgagct ggccatcatc gttcagcaag atcagcgccg ccgcgtcagc gccgaccaga
    29041 tccgccgccg cgcccgccgc cttgcgaaag aacgcctcga aatcggcctc cagcgacagc
    29101 gcctcgacca cctgacgcag ggcacggatg gcggtgaaag tgtcggtgtg acggatgatc
    29161 atgcaatggc tgcttctcgc ggtgatggcg gtaaccgatg tggcgcataa aatgcaatat
    29221 ttgtgcccgc agcacgcttc ggcggcagtg cacagtcaca acaacacacg cccatcactc
    29281 tggcccggaa ttggatcacc accccaaccg catacgcacc acaacagtgc tgacgttcgc
    29341 tgaaaaccga tcagaggctc gcgagagaac acacagcagg acgctttgtc ggcgagaaga
    29401 ggaatcaggc ggggcgcagc ggttacagga ttcgtcgcac cctacccctt cgcttccagc
    29461 gctccgaaga agccgaccag gcgttgcagt tgaccggtgg gagacagctc gccgaagtcg
    29521 atcccttcga caaagagcgt tccatcggcg ttcaacatgc gccagttgaa gcgaaagtag
    29581 cgatcgtgcg cgtcgatctc gctggtacgc tcgagacgtg accccggata ttgctgaatg
    29641 aacgccccca tgtggtcgac aagcgccatt ctgccggcca cgagaccgaa cgggtcggca
    29701 taggtgccct gctcatccca gaccgattcg agcagttgct gccgacgcgc gcggtcgggc
    29761 tggctccagg catcggcgta gtgcgcgatc agttccgaca gttccatgat tgacctccgc
    29821 tgtactgggt gggtgctcca gtgtgcggca tcggcaccgg tcggacaatc cgcagggatt
    29881 cagcgcaacg gcggctcggg tgtgaagccc tgaatgtcgg cctgctcgat cagctgggcg
    29941 atgtagcggg cgatggcgcg gcgctgcgcc tgttcggcga ggcgggcggc gatggtggcg
    30001 ctgaccgcct cgaacggcag gcgctggggt tcgccgcgcc ggagcaggcg gatcagatgg
    30061 aagccgtagg cagtctccag cggggcggag agctcacccg gtgcgaggca ttgcagggcg
    30121 gcttcgaacg gtggcgtggt ctcgcccggg ccgagctggc cgagacggcc gccgtcggcg
    30181 cccgaggcgc agtcggagaa agcgcgcgcc agttcggcga accgctccgg ggcctgttgc
    30241 agccgtgcat ggagttgctc cgccttggcg cgtgcggcgg caaaggcgtc ggcctgatca
    30301 cggcgcgcgg cgaccaggat gtgctcggcc tcatgcagca gcggggtcat gaaacgctcg
    30361 gggtggtcgt cgtagtggcg gcgcacctgc tcggccgggg cttcgcatgg cggaacctcc
    30421 cgttcgatca gggtgcggat cagcgcatcc tcttcggttt cgatgccgcc ctggggctcg
    30481 gcctgcggga tggcgtcaag cgccatccgg cgcgcctgct gcagcagcag ttcgcggatg
    30541 atcagcgccc gcgcggcctg ctgccaactg ctctcgaact gatcgcccgg aaagtgctgc
    30601 atctcggcgg cgatgcgctg cggcgcgatc ggctggccgt tgaccgtcag catcgctggc
    30661 gtggcgtgtg ccgcctgggg cggatccgcg tccaggttga acagtggaat gacgccctga
    30721 ttcggaaaat cggctgcgtt gtgctgcatg atccgttcct caggcattgc gcggcggctg
    30781 ggcgcggcgg cgcacgatct ggtagccggg ccgaccgagg taccagatcg gcgcgctcca
    30841 gatgtgcacc agccgggtga aggggaacag caacagcagc gtcagcccca acagcaggtg
    30901 ggccttgaag atcggatgga catcggcgac atggaccgcg gcagccggtt gcagggtgac
    30961 gatgccctgg gcccacgcca tgaacttgac catttcgtgg ccatccagat ggccgagcga
    31021 gacgaagatg gtgcagagcc ccagcgtgag ttgcagccag agcagcagca ggatggcgat
    31081 gtcaccgacg ctcgaggtgg cgcggatgcg tgcatcgaac agccgccggt gggccaacag
    31141 cgtgatgccg atgaagcaga ccacgccggc cagaccgccg acgacgatgg cgagcgtctg
    31201 cttgaagccg tggctgatgc ccaactgatc gaagatctcg atcggcgtga gcaggccgac
    31261 gaagtggccg gccagaatgg tcaggatgcc gacatggaac aggttcgatc cccagcgcaa
    31321 ctgccccttg cgcagcagtt gcgaggagcc gctcttccag ctgtactgct cgcggtcgaa
    31381 gcgcagcagg ctgccgagca ggaagatggt caggcagaga tagggatacc agccgaacag
    31441 ggtcatattg agggtcgcgg acatgggtgc ttctccgtcg gattcaggtt tgccgcgccc
    31501 gtcggggcac ggctgacagg ggaacgctgg ccaccgtcgc gcccgcgcct tcgcaggccg
    31561 ccgcgccggg accgaagatc accggcggct cggcccactc ggcatcgagt tcggccagca
    31621 gatcgctcgc caggttgact gccacggtag ggagcgactg gtcgccggct gcgctgcgaa
    31681 tggcccgcag caggctggca tagccgctgt cgcgtgcccg cagccgctct tcgagggcgg
    31741 cgaggatctg cagcgtctcg gccagcagcg attgggcctc gtcgagcggt cgggtcgaga
    31801 ggtattcgag gaacagcggc aggtagtccg gcagctcgtt gtcgacccgc tccagtccgc
    31861 cctgttcgta gagcgcagcc agatcgacca gcgcctggcc gcggtcgcgg ctctcgccat
    31921 ggacatgctc gaacaggtgg agtgacaact ggcggctgcg gtcgaacagc gccacgtagt
    31981 gctcctgcag gtcgatcaga tcgctctgct cgatgaaggc gcagaagccc tccagctcgg
    32041 ccagaaccgc cggcgcgatt tgcccttcag ccgcgatgag gccgcgaatc tcggcgatgg
    32101 ccgcctgcaa cgtctcactc ggatagcaga gcagcgccga cagcgccttg aaggtgaggc
    32161 tcatttcagc acctccccgg ggttgtgcga ttgactgccc tgcgccgggc tgaacagttt
    32221 caccggcgtg aacagctcgg ccttcgaact gccgccggag cagccgttgc cgaaagagaa
    32281 gccacaattg ccgcgcaacg cgtagagatc ctcgtgcgcc tcgcggcggg cggtggggat
    32341 gacgaagcgg tcttcgtagt tggcgatcgc catgatccgg tacatctcct cgatcaccgc
    32401 gcccgacagc ccgacccgct cggcgatcga ctcatcgatg cgcccctcga cgctcttggc
    32461 gcgcatgtag ccgcgcatcg ccagcatccg ctccagcgcc aaggcgacgg gcgcctcgtc
    32521 gccggcggtc agcatgttgg cgaggtagcg cagcgggatg cgcagcgagc gcacatccgg
    32581 caggccggcg tcgatgccga tctgcccggc ggcggccgcc gactggatcg gcgacagcgg
    32641 cggcacatac cagaccatcg gcagcgtgcg gtattccgga tgcagcggaa aggcgatctt
    32701 ccactccatc gccatcttcc acaccggcga atggcgggcc gcctcgagcc agctctcagg
    32761 aatgccgtcg gcgcgcgcct gggcgatcac cgccgggtcg tgcggatcga ggaagaggtc
    32821 gagctgcgcc tggtagagat cacgctcatc cgctgcgctg gccgccgcct cgatgcggtc
    32881 ggcgtcgtag agcagcacgc cgaggtagcg gatgcggccg acgcaggtct cggagcacac
    32941 cgtgggctgg ccggcctcga tgcgcgggta gcagaagatg cacttctcgg ccttgccgct
    33001 cttccagttg tagtagatct tct
//
LOCUS       DAORCQ010000020        32597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_726_length_32597_cov_5.119323, whole genome shotgun sequence.
ACCESSION   DAORCQ010000020 DAORCQ010000000
VERSION     DAORCQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 32597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32597
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_32597_cov_5.119323"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKI23_03500"
     CDS             complement(<1..773)
                     /locus_tag="PKI23_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNN36039.1"
                     /translation="MTHPDQPNRTRTTCAYCGVGCGLEVSVAASNAGQPVPRVELRGD
                     AAHPANHGRLCSKGSALGETLGLDNRLLHPQIGGLRASWNLAIDTIANRLRDIIAESG
                     PEAVAFYVSGQLLTEDYYVANKLMKGFIGAGNIDTNSRLCMSSAASAHKRAFGSDSVP
                     GCYEDLELADLILLTGSNTAWAHPVVYQRIVKAKAERPQLKVVVIDPRRTASCDLADL
                     HLPIAPGSDALLFNGLLADLARRDRLDPAYIAAHTEGFEA"
     gene            complement(803..2047)
                     /locus_tag="PKI23_03505"
     CDS             complement(803..2047)
                     /locus_tag="PKI23_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNN36040.1"
                     /translation="MQPERLIIIGNGMVGSRLLEELLKRDRDRYHITLFGAEPHVAYN
                     RILLSSLLAGDKSEAQIMFHDRSWYQQQQITLHLGETVTTIDPIKHLVRSDRGTCLHY
                     DKLVLATGSRPMVPPIPGHDLPGVIALRTLDDTRRLLDACTDRHHAVVIGGGLLGLET
                     AHGLLRQGMAVTVVHNSTHLMNRQLDETAARLLQGHLEQQGIRFMLNADTRVIEGDPL
                     QGVSGVAFADGSRIDADLVVMAIGIRPNVDLARQTGLAIGRGITVDSQLRSSDPAIFA
                     VGECVELHGELFGLVAPLYQQARALAEHLTGGSDDHFVPAVTSTQLKVTGVDLFSAGD
                     FTESTQRQTLLYSDLACGIYKKLVLQDDRLIGAVLHGAVEDGNWYFDLIQNGTSVAGL
                     RELLIFGSAFCAQEGAGTLQAA"
     gene            complement(2064..2432)
                     /gene="nirD"
                     /locus_tag="PKI23_03510"
     CDS             complement(2064..2432)
                     /gene="nirD"
                     /locus_tag="PKI23_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009407445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNN36041.1"
                     /translation="MSIHTMSTPLSQQWHPVCPLEEILPDSGICAAIDQRQIALFRVG
                     DGSDLYALDNYDPIGKANVLSRGLVGDLRGTLVVASPLYKQHFSLASGRCLEQEEVAV
                     ATYPVRVNDGWVEVALTTAE"
     gene            complement(2429..4978)
                     /gene="nirB"
                     /locus_tag="PKI23_03515"
     CDS             complement(2429..4978)
                     /gene="nirB"
                     /locus_tag="PKI23_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNN36042.1"
                     /translation="MKKQNLVVIGNGMVGQKLLDQLAESGATARYAITVLCEEPRPAY
                     DRVQLTAYFSGSSAADLALVEQTFFDTHGIALQLNCRSVAIDRDKRQVHSAAGACFDY
                     DKLVLATGSYPFVPPIPGHDRDGCYVYRTIEDLDAIRQGASGRSSGVVVGGGLLGLEA
                     AKALKDLGLKTHVVEFAPRLMAVQVDEGGGRILRGKIEALGVTVHTEKNTQEIVDGSG
                     MRHRMRFVDGSELESDLIVFSAGIRPRDELARQAGLEVGPRGGVVINDWCQTSDPNIY
                     AIGEVALWDGRIFGLVAPGYQMARVAASHLQGSEVVAFTGADMSTKLKLMGVDVASIG
                     DAHARTPGARSYTYIDEAKEIYKRIIVSGDGKKLLGSVLVGNSDEYGTLLQQVLNDIP
                     LPENPDQLILPSYDGSAAKGLGVDALPDSAQICSCNNVSKGALCGAIAGGGVTLAELK
                     ATTRAATSCGGCSALVTQVLNAELVKQGIEVKRDLCEHFPYSRQELYHLVRVGEIRSF
                     RALIGKHGRGLGCDICKPVVASILASCWNDHVLDGQHAVLQDTNDYYLANMQKDGSYS
                     VVPRVAGGEITPDKLVAIGQVAMKYDLYTKITGGQRIDLFGARVEQLPLIWKDLIEAG
                     FESGHAYGKALRTVKSCVGSTWCRYGMQDSVGMAILVENRYKGLRAPHKLKMAVSGCT
                     RECAEAQSKDVGVIATERGWSLYLCGNGGMKPRHADLFATDLDDAALLHAIDRFLMFY
                     IRTADRLQRTSVWMDNLEGGIDYLRAVILDDSLGIGAELEQQMQLIIDTYQCEWKSAV
                     EDPAKLKRFRHFVNSDAGDSNIRFVQERGQKRPISERERNLENKPRIKLVQ"
     gene            complement(5027..6700)
                     /locus_tag="PKI23_03520"
     CDS             complement(5027..6700)
                     /locus_tag="PKI23_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="This model describes the ATP binding subunits of
                     ATP-binding cassette (ABC) transporters for nitrate
                     transport, or for bicarbonate transport, in bacteria and
                     archaea; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HNN36043.1"
                     /translation="MSLLNLRNISKSFGAGSQKSEVLKQINLSIDEGEFVAIVGFSGS
                     GKSTLINTIAGLIFPDSGEALVRDEKIRGPGPERGMVFQSYSLMPWLSVYDNVALAVN
                     QVHRSWSKAQRDAHIRKYIALVNLTAATEKRPAELSGGMRQRVAVARALAASPDILLL
                     DEPLSALDALTRGNLQEEILRIWEEEKKTVILITNDVDEGLLMADRVIPLTLGPGATF
                     GPEFVVDLPRPRDRVEMNHNARFKTLRGEITQYLLALGQGKQEQGSRNEIELPPVRPN
                     TSNDFARDLSTLKESPRNLGQARYLEFANLSKIYPTPKGPVAVVKGFDLLMKKGEFVS
                     IIGHSGCGKSTVLSMAAGLNDISEGGIVLDGKEIDGAGPDRAVVFQAPSLFPWMSAFD
                     NVALGVEQVYPHATPNERDDIVEYYLTRVGLANSMHKRASELSGGMKQRVGIARAFAL
                     SPKLLLLDEPFGMLDSLTRWELQEVLMEVWSRAQLTAIMITHDVDEAILLGDRVVMMT
                     NGPEARVGKILEVDLPRPRSRRELLSHPRYYELRESLIQFLAECDHARH"
     gene            complement(6729..7751)
                     /locus_tag="PKI23_03525"
     CDS             complement(6729..7751)
                     /locus_tag="PKI23_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN36044.1"
                     /translation="MTARLVRFFDLIGLQFMVPMTRLAAGESPRQQARELWMMVGVPL
                     VALGIFLLLWHLGAARIQTSLGTIPGPLQVWTEFTGLLDEHRAERLRETEFKQRQIER
                     NKALLAENPQAKPAPLRAYTGKATYLDQIATSLMTVFAGFLLATLLAVPIGILCGLSR
                     VINSALNLLIQIFKPVSPLAWLPIVTIVVSALYTNTSGGWMSKSFIVSSITVTLCSLW
                     PTLINTAMGVASIDKDLMNVGRMLRLNWFTQVRKIVMPSALPLIFTGLRLSLGVGWMV
                     LIAAEMLAQNPGLGKFVWDEFQNGSSQSLGRIMVAVFTIGIIGFALDRLMLTLQKLFS
                     FGQNTP"
     gene            complement(7867..9255)
                     /locus_tag="PKI23_03530"
     CDS             complement(7867..9255)
                     /locus_tag="PKI23_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNN36045.1"
                     /translation="MSRSNHLSSRPLWQRIITTVALSAAGLLPVTLVSAAGPAAEKSE
                     LKFGFIKLTDMAPLAVAYEKRYFEDEGLYVTLEPQANWKVLLDRVIDGQLDGAHMLAG
                     QPLGASVGIGTKAEVITAFSMDLNGNGITLSNEVWQKMKPALPLGADGKPLHPIKADA
                     LKPVVAEFRAQGKPFKMGMVFPVSTHNYELRYWLAAGGIHPGYYSANDPAGLTSAEVQ
                     LAVTPPPQMPVTLEAGTINGYCVGEPWNQQAVFKGIGVPVITDYEIWKNNPEKVFGVT
                     KTWAEQNPVTHKKVLKALIRAAIWLDENNNANRAEAVKMLSRAEYVGADAAVIANSMT
                     GTFEYEKGDKRAVPDFNVFFRHQATYPFYSDAIWYLTQMRRWGQIAEQKPDEWYAQTA
                     KQVYRPDLYLAAAKELVSEGHAKQEDFPWSSDGYRPAQSDFIDGVRYDGKQPNAYLEQ
                     FKIGLKGAQSAQ"
     gene            complement(9300..10595)
                     /locus_tag="PKI23_03535"
     CDS             complement(9300..10595)
                     /locus_tag="PKI23_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNN36046.1"
                     /translation="MSTDLEKRRLKLGFLPLTDCAPLVVAHELGLFEEFGLEVELVRE
                     ASWASLRDKVAFGLLDGAQMLSSLPIATTLGLGGIQKTMLTGCCLSLNGNAITVSNTL
                     FQRLLAVDGSTSQSDAVDHGWRLRQLIERDRQAGRPPLRFAHVFPTSNHHYQLRHWLT
                     AAGIDPDRDLSLLVIPPARMVEHLRDGSIDGCCVGEPWGSLAVCEGVGQLLTSGHAIW
                     NNSPEKVFGVTAQWHQQHPASHHALLKALLLACDWLDRPRHRNEAAQLLARPEYVDAP
                     AEVLEMALTGNFRAGQQKSALNCGDFIVFSRYAANFPWLSHAEWLLTQMYRWGQLDQA
                     LDLRELAGQVYRPDLFRAAATELGLPVPTIDRKSEGSHAGHWMLSEASTPLLMGPDQL
                     IDGSRFDPAQPIAQLLTQPPPAQLRVDLAALAALNHIPH"
     gene            complement(10927..11520)
                     /locus_tag="PKI23_03540"
     CDS             complement(10927..11520)
                     /locus_tag="PKI23_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNN36047.1"
                     /translation="MTPTKPTLRILVIDTSPARSSALLQALESAGYQVVALLHSHQLL
                     REQIERHQPDVILIDMESPDRDTLESLHEVHCNQPRPIVMFSDANDSALIEAAVQAGV
                     SAYVVDGMQPHRLRPIIEVAIARFREFQALRDELQQTRNKLAERREIEKAKGILMKQK
                     GMAEEEAYQALRKLAMDQGLKIGEVARQLISIAKLLG"
     gene            complement(11734..13329)
                     /locus_tag="PKI23_03545"
     CDS             complement(11734..13329)
                     /locus_tag="PKI23_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN36048.1"
                     /translation="MVERTLLRLLVLDGEDDFAQLAINRLRKAGQPLRAQRIEDELQL
                     ERALMQPDEWDLLLAPEHCPFVDPAALTGLLAGRQIDLPVLLESASDESAHITTLLQQ
                     GIAEVIPAGDDLRLNLLMQRQHQQLLDRRAAKSLAQQLQVAEGRAEHLANLAALPLAY
                     LQDGMHMQANPIYSALFGQIDHEELLATPFLDLIAPRSVEPFKAHLRLLNEADEQQVI
                     PPLTLTLQTVDGRDFTALLHFAPTRYQGESCLQLWVEPKVAPPLAAPEPLEVAEETAA
                     RSTDSEEIHTTLIRTLEQAIDGHLLSLLFEPIVGLHGAGTELYECHLTLIDQQGVEHP
                     CSSLWSWADAGGLARKLNHWLLLNAIKRLVEHRDQGHQAALFVNLPASALLDEGLTAW
                     LSVALRAADLPAHSLVIQIDEQDTAHHLKESIAFAERLKTLGCPLSLRQFGRSEDPFR
                     AFRQINPDYVQIAPELLHDSERLQKQVRELQSQGKLIIVPAVRSATVMPMLFQLGINF
                     IEGSYLRPPMSRMDFDFGLDEAV"
     gene            complement(13395..15062)
                     /locus_tag="PKI23_03550"
     CDS             complement(13395..15062)
                     /locus_tag="PKI23_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36049.1"
                     /translation="MTELNQLVTTAAVRLALLELLRPPTQQLSMLLERHFSNQPLQLR
                     NAALETARLAQAMQALLWRGYRQVLVDADNEGLLTPGRSAAVPALVVSAIARAIAAQS
                     EILLRSSQLYAPTPAGFWQALHQLHLLAERLDCTAVEVPDPLLQQRSGLSIEAAYLRV
                     LLFDIAQPQQMRQQSCLPLYRALELWSREARLLPLQQTPASHHFRVDLQSDLPAIYTD
                     HERPITPGTWRLLDLKPILERLEQIAATPTDQPGPIERELVDQLFVSWGARATRRFQR
                     IESHEQVEIIIGLPSIHFHCANRLDFSEWLNRLLPRLELTQLPPEKGSPFAAPSADVW
                     DGAFDAREKFNPLIHLQLPPVLEFTRPVTAAAEQTLPHQPSYPIHQVQVANSSVNGYR
                     LRWHQSIPERIQNGTLLGIRTEPSLPWSIATVRWTQLDERSQAHVGVELLGPNATACA
                     VRLEGKRGEVTEFMRGLLLPTLPTTGQAASLLLFCKPFRSKDKVTLIQPGQRNSIKLV
                     RQLRKNHTFAQFEFLALQACARTENTEKIERNLPIPASGFDQLWQIL"
     gene            complement(15377..16408)
                     /gene="epmB"
                     /locus_tag="PKI23_03555"
     CDS             complement(15377..16408)
                     /gene="epmB"
                     /locus_tag="PKI23_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HNN36050.1"
                     /translation="MPDSKPPIIPLSTHCWHGPSWQQLLREAPLSLPELARRLKLDPA
                     QLAGDATTARQFTLRVPAPYLARIEPGNPDDPLLRQVLPVAEELLARPGFTSDPLDEA
                     HHSPLPGVLHKYRSRLLIVTTGACAVHCRYCFRRHFPYQAHQQGRTGQQAVLEYLAHH
                     PEISEVILSGGDPLSLSDTVLERWIEQLARLPQLLRLRLHSRLPVVIPQRITAELLDI
                     LTGSRLTTVMVLHSNHPNEIDGDVVSAAQRMRAAGITLLNQSVLLRGVNDDAGTLAEL
                     SEKLFVAGILPYYLHLLDPVAGAAHFAVPEPEARQIHQSLLARVSGYLAPRLVVEVPG
                     ERSKRPLGS"
     gene            16482..17051
                     /gene="efp"
                     /locus_tag="PKI23_03560"
     CDS             16482..17051
                     /gene="efp"
                     /locus_tag="PKI23_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNN36051.1"
                     /translation="MANYSTNEFRAGIKVMLDGDPCAIVENEFVKPGKGQAFNRVRLR
                     NLKTGRVWERTFKSGETLEAADVTDRNLQYLYTDGEQWHFMDNDSFEQYAVDKRTVGD
                     AVQWLREQDVCIVTLWNNAPLAVTPPNFVELEIVETDPGIRGDTATGGTKPARLSTGA
                     VVKVPLFVNQGEVIRVDTRTGDYQSRVKE"
     gene            17048..18025
                     /gene="epmA"
                     /locus_tag="PKI23_03565"
     CDS             17048..18025
                     /gene="epmA"
                     /locus_tag="PKI23_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HNN36052.1"
                     /translation="MSQAPAAGGDWQPSASLAALRQWAELLSRVRAFFARRALLEVTT
                     PLLCTTTATDPQLVSFVVTEGEPLPRFLQTSPELAMKRLLAAGSGPIYQIAKAFRQHE
                     RGARHNPEFHLLEWYRPGFSLERLMDEVAELVAELTGRHHFERLSYRTLFARHLDLDP
                     FVTPTEELEHTARRQIDLGPTVAGDRQFWLDLLLTHLIEPRLASIPALFIHDYPPEQA
                     ALARLRRDECGVWVAARFELYLDGIEIANGYDELTDADEQRRRFIADNQRRQQQGQPQ
                     LPVDERLLAALAAGLPATAGVALGLERLAMAATGQRRIDEVLAFTFERA"
     gene            complement(18039..18884)
                     /gene="asd"
                     /locus_tag="PKI23_03570"
     CDS             complement(18039..18884)
                     /gene="asd"
                     /locus_tag="PKI23_03570"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699415.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNN36053.1"
                     /translation="MRDSLFILLQHLLPQKLLSRLMGLLADRPLGALTQWLIRLFVRR
                     YQVDLAEAEPSTPTGYASFNAFFTRPLRAGARPIAAEPHSLVSPADGQITEHGPLLGQ
                     RLVQAKGRHYTLQALLAGDQQAVEQFRDGDYCTLYLSPRDYHRVHMPVDGRLTSMVHV
                     PGRLFSVNQLTAERVEDLFARNERVICHFDTDEGPLAVILVGALIVGSIETGWAGVVT
                     PPGRGISRWQGGTPVALKRGDELGRFRLGSTVILLLPPGRMRWNANLTQQPVRMGQAL
                     GQFKG"
     gene            19298..20056
                     /locus_tag="PKI23_03575"
     CDS             19298..20056
                     /locus_tag="PKI23_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HNN36054.1"
                     /translation="MKLRTLLLDLGGVFYQGDHLIPGALEALARLRASGLALRFVTNT
                     TRQSRAALQGELAGRGIAIAEDELLTPAAAARDTLLARRLRPLLLIHPDLAPDFAGLD
                     TAEPNAVVVGDAGEHFSYTGLNQAFRLLQAGAPLLALGRNRYFQERDGLSLDAGPFVA
                     ALEYAAGVQAELLGKPAPALFAAALESAGCPPTAAAMIGDDVEADINGALAAGLRGVL
                     VRTGKYRPGDEAHLAPGGELADDVGTAATLLGIA"
     gene            complement(20678..21565)
                     /locus_tag="PKI23_03580"
     CDS             complement(20678..21565)
                     /locus_tag="PKI23_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNN36055.1"
                     /translation="MHDLSETPEHRGHEREAVLFNAAVRALGAFGYSTLEFDIGGKKA
                     RRRYVSAIAPNGEPVNIWIKSATQWPGLAEAVRFPWKKLSVSPDGLSAISVACEDAAK
                     RGATHFLAIVGNDRSGVLSFARLYSISEVPTLAATQSTQINSVFYRAHSAALILEARA
                     PEFVEAAKAACAIGEDILSAPHREQSTGATQVVLRARSGNVYRRDPRVREQVLKIARG
                     RCECCGELGFLTESGERYLETHHVVGVSERGPDSPDNIVAVCPLCHRKAHFSAEKTEV
                     ERKMLEAIRRRGRPSAAQA"
     gene            complement(21809..24418)
                     /gene="alaS"
                     /locus_tag="PKI23_03585"
     CDS             complement(21809..24418)
                     /gene="alaS"
                     /locus_tag="PKI23_03585"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNN36056.1"
                     /translation="MNSSELRRAFLDFFAEQGHEVVPSSSLVPGNDPTLLFTNAGMVQ
                     FKDVFLGQDVRPYRRAASSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIHYAWRFLTERLGLPKARLWVTIFHEDQEAERIWLDEIGIDPQRFSRLGDASNFW
                     SMGETGPCGPCTEIFYDHGSEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRLPDGRLEP
                     LPKPSVDTGMGLERIAAVMQGVHSNYQIDLFRHLLEAAARITGRQDAHTNDSLKVIVD
                     HIRSSSFLIADGVIPGNEGRGYVLRRIIRRALRHGHKLGVKQPFFHRLVAALAQQMGA
                     AYPELPARQAQIERILLAEEEQFSRTLDQGMRILDEAIAQLNGACIGGETLFQLYDTY
                     GFPLDLTADIARERGLQIDSAGFEAAMQAQRERARAASQFALDSTALIRQSDHECLFT
                     GYEQTADDAEVVALYRDHEPVERLSHGETGWVLLDRTPFYAEAGGQVGDRGQLRAGGG
                     RAEVLNTLKQGNNHLHQVRLLQGNLEAGSRLTAEVDPARRQATALNHSATHLLHAALR
                     EILGSHVEQKGSLVDAQRLRFDFAHFEAIAPSTLRQIEQRVNDEIRRNSPISVELLPI
                     DVAKARGAMALFGEKYGAEVRVLTMGDGFSVELCGGTHARRTGDIGLFRITAESGISA
                     GVRRIEAVSGAAALELAQYEANLLRELAQQLRAPIDQLPEKLTALLDEQRRLERIVQQ
                     LGARLASAASADLLDRLVQVQDVPLLVAAVDGMEAKGLRDLLDKVRDKLGSGVILLAS
                     VSAGKVNLIAGVTADLTARIAAGELIKAITALMGGRGGGRADMAQGGSDQPDALDAAL
                     AEAPAIVARLLSA"
     gene            complement(24482..24961)
                     /locus_tag="PKI23_03590"
     CDS             complement(24482..24961)
                     /locus_tag="PKI23_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNN36057.1"
                     /translation="MPSDPPQPAKTRPLRHQALDLLARREHASHELATKLLAKGGDRT
                     EIETVLTQLQQEGLLSDARFAEMRCRQRREAGFGPLHVSSDLLARGLSRQLITEVIDA
                     ESELWLSAARHAKQKRFGPGRPRDPATQAKWMRFLQARGFTQRQIRHALHDTEAEED"
     gene            complement(24976..25485)
                     /locus_tag="PKI23_03595"
     CDS             complement(24976..25485)
                     /locus_tag="PKI23_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNN36058.1"
                     /translation="MVTIKLKRILTHLWSGERPLRRAFPASALRQIEQAIQRSEASYS
                     GELRFVVEAALDIGPLWAGTSAADRALELFSQLRIWDTEQNNGVLIYLLLAERDVEII
                     ADRGIHRRVGSAGWAQICRMMEQAFGRGAYLEGSIAGIEAIAQQLALHFPAQDQANPN
                     ELPDAPLLL"
     gene            complement(25479..26348)
                     /locus_tag="PKI23_03600"
     CDS             complement(25479..26348)
                     /locus_tag="PKI23_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HNN36059.1"
                     /translation="MNRLGLPKSMAGLLLTALLGIVWISHAELPVPPLTAPVIDQTAT
                     LTPEQQRNIEQRLRSFEQRKGSQIALLLVATTQPESIEQYSMRVVEQWKLGREKIDDG
                     VLLIIAKDDRTLRIEVGYGLEGVLNDALSKRIISELITPRLRQGDFYGGITAGIEQII
                     RLIDGEPLPPPSQRPARDRLEIANLLPLLFIIVLMAGGALRALLGRPLGALVSGTLIG
                     LLTWFLAGALLIALLAGIGAFLFTLLGHAGRGLGGFPGGFGGRGGFGGGGFGGGFSGG
                     GGGFGGGGASGRW"
     gene            complement(26345..26956)
                     /locus_tag="PKI23_03605"
     CDS             complement(26345..26956)
                     /locus_tag="PKI23_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNN36060.1"
                     /translation="MHPMRIWLIALLTLILSGCGYNTLQSQDEQIKAAWSEVLNQYQR
                     RADLVPNLVNSVKGYAAQEKAVLTEVTQARASVGSIQLTPELLNDPAAFARFQAAQGQ
                     LTSSLSRLLVVAENYPQLKSDANFRDLQAQLEGTENRITVARNRYIKAVQAYNVTVRS
                     LPSNLTAMLLGYHVKPNFSVENEQQISTAPKVDFAPAPDSKSR"
     gene            27177..27998
                     /locus_tag="PKI23_03610"
     CDS             27177..27998
                     /locus_tag="PKI23_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36061.1"
                     /translation="MLTLASLLLPTASWAARYQAVVQSPNSSGTAGEVRNVVLAFDDQ
                     WNYLTADFTILPSENGGVANGFWMVVSPANATGNAARSLTLYADLNTHQVSLYRLSGD
                     PLNFAVDRNKDFLSSHANAVRVSDEETSGRRVVRIAVPLMVENGLGRFGAGGVSWQMQ
                     LLLDARFSYDANHHLLGVKSSAQSDSQVQAQPVARLNSPAEAPQLAGSKESPLWMLLF
                     VFGLGGLIWVWRHPRYRRHVRQSEQRAAAAAAAAKVTTASVDAVESDRLIAAPKP"
     gene            complement(27937..29022)
                     /gene="dinB"
                     /locus_tag="PKI23_03615"
     CDS             complement(27937..29022)
                     /gene="dinB"
                     /locus_tag="PKI23_03615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNN36062.1"
                     /translation="MSQINESTRKIIHIDMDAYFAAIEQRDHPQWRGKPLIVGGAPNS
                     RGVVATCSYEARRYGIHSAMPSARAYQLCPQALFVKPRFEIYQQVSLQIREIFQHYTD
                     LVEPLSLDEAYLDVTGSTLHRGSASLIAQEIRQRIRQKTQLTASAGVSYNKFLAKLAC
                     ERGKPDGLFVIMPSEGAAHAAALPIDRFHGIGRSTASRMRSLGIEFGADLLRFGLPEL
                     EQHFGKLARHYHGMAAGIDPRPVNPSRIRQSIGAETTFADDLTDSAQMVSELERLLDR
                     VTQELQQTGQRAQTLTVKVRFSDFRSVTRGMTLRQGFASAPQVDSVLKTLLQRALTDF
                     RPVRLLGITVSGLQSADHSQPRQLTLW"
     gene            29180..29479
                     /locus_tag="PKI23_03620"
     CDS             29180..29479
                     /locus_tag="PKI23_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNN36063.1"
                     /translation="MNSPQLAMQYQAAGAADPVWIASQPDRLMLSEVRATEQVTLLQI
                     AGGNQVTLRLLSMGIRPGQQIQVLHNRTGAVVIAAGSGRIAVGKRLAQQITVQRG"
     gene            29491..31809
                     /gene="feoB"
                     /locus_tag="PKI23_03625"
     CDS             29491..31809
                     /gene="feoB"
                     /locus_tag="PKI23_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HNN36064.1"
                     /translation="MRQYTIAVVGNPNCGKTTLFNALTGARQRTGNWPGVTVDRKEGQ
                     FEIDGQPIRLIDLPGVYSLDRFSQALDEKVACDYLLAGEADLLINILDAANLERSLYL
                     TAQLIEMGAPLLVVLNMMDHATAQGLTVDPLQLSQQLGCPVVVTVASREQGIAQLKTA
                     IAERLTHPTTRGDGILHPVQIEFAVATLVDRLDPLGRDPARRWLALELLANEGVAPAV
                     EPELLHDVAEMRRQISTELHEDVELLIADSRYGFARRVAQEVATRGQAPGSTSSDRID
                     RIVLNRFAGVPIFLGVIYLMFLFSINFSGALIDFFDQVTGALLVDGLGQLLLALGLPQ
                     WVKVLLADGLGGGVQVVATFIPVIGALYLFLSFLEDSGYMARAAFVMDRLMNRIGLPG
                     KAFVPLIIGFGCNVPAIMATRTLESHRERILTSMMAPFMSCGARLSVYALFAAAFFPV
                     GGANVVFSLYLLGILIAVLTALLLKNSLLKGEADAYLLELPAYHLPTLKGICIHTWDK
                     LKGFVVRAGRIIIVMVLVINLLNSIGRDGSFGNENSENSLLSWIAREGTPLFAPMGLR
                     EENWPAVVGILAGVLAKEVVVGTLDSIYSRMDRESAGEADAATPFDLLAQLSAAAATI
                     PQNLLQLLDRLGDPLGFSVIKEGDPSAAAAEQQVSLSTFGAMVSRFDGQAGAYAYLLF
                     ILLYFPCVSTIAALRRETGTGWTLFAAGWGTFIAYTSATFCYQLASFARHPGSSSFWI
                     ALVLLSWVAVVGVFRWLGRSAPSTASVKAGAA"
     gene            31806..32078
                     /locus_tag="PKI23_03630"
     CDS             31806..32078
                     /locus_tag="PKI23_03630"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HNN36065.1"
                     /translation="MNLIQLKNHLKQHHECSLRDLALRFDSSAEVLRHQLALLERKGL
                     VKKLPSGTLCSSGCRSCATEQIELYRWVAAEEGSAVPLRFGNSGRN"
     gene            complement(32009..>32597)
                     /locus_tag="PKI23_03635"
     CDS             complement(32009..>32597)
                     /locus_tag="PKI23_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNN36066.1"
                     /translation="TLLVLGDSLSAGYGLTLDQGWVELLKQRLAAEAFPVEVINASIS
                     GETTDGGLRRLPDLLDRHTPSLVIIELGANDGLRGFALDSIRANLTRMVQLVQQRGAR
                     PLLVGIRIPPNYGSRYSEPFFIQYQEIAKENSIPLVPFLLEGVAAQPGMMQADGLHPT
                     AAAQPRLLDTVWLQLRPLLPKRSGTALPSSAATQR"
BASE COUNT         5720 a        10061 c        10679 g         6137 t
ORIGIN      
        1 gcctcgaaac cttcggtgtg ggccgcgatg taggccggat cgagtcggtc gcggcgggcc
       61 aggtcggcga gcagtccgtt gaacagcagt gcatcgctgc ccggggcgat cggcagatgc
      121 aggtcggcca ggtcgcaact ggcggtgcgg cgcgggtcga tcaccaccac cttcaactgc
      181 ggtcgctcgg ccttggcctt gacgatgcgc tgatagacca ccggatgggc ccaggcggtg
      241 ttggagccgg tcagcagaat caggtcagcc agctccagat cttcatagca gcccggcacg
      301 ctgtcgctgc cgaaggcgcg cttgtgggcc gaggcggccg aggacatgca aagacgcgag
      361 ttggtgtcga tgttgccggc gccgatgaac cccttcatca gcttgttggc gacatagtag
      421 tcctcggtca gcaactggcc ggagacgtag aaggccacgg cttctggacc tgactcggca
      481 atgatgtcgc gcagccggtt ggcgatggtg tcgatggcaa gattccaact ggcacgcagg
      541 ccgccgatct gcggatggag cagacggttg tcgaggccga gggtctcgcc gagcgccgac
      601 cccttggagc agaggcggcc atgattggcc ggatgggcgg catcgccgcg cagttcgacg
      661 cgggggacag gttgtccggc attgctggcg gcgacgctga cctcgaggcc gcagcccacg
      721 ccgcagtagg cgcaggtggt gcgagtgcgg ttgggttgat caggatgggt cacgatgcgc
      781 tccgcggatt gacacttcag cttcaggcgg cctgcagcgt gccggcgccc tcctgggcgc
      841 agaaggcgct gccgaagatc agcagctcac gcagcccggc caccgatgtg ccgttttgga
      901 tcagatcgaa gtaccagttg ccatcctcca cggcaccatg gaggacggcg ccgatcagtc
      961 gatcatcctg cagcaccagt ttcttgtaga tgccgcaggc caggtcggag tagagcagcg
     1021 tctgccgttg tgtgctctcg gtgaaatctc cggccgagaa gaggtcgacg ccggtcacct
     1081 tgagctgggt cgaggtgacc gctggaacga agtggtcgtc actgccgcca gtcagatgtt
     1141 cggccagcgc gcgggcctgc tgatagagcg gcgccaccag tccgaacagt tcgccatgca
     1201 gttcgacgca ctcgccgacg gcgaagatgg ccgggtcgga gctacgcagt tggctatcga
     1261 cggtgatgcc acggccgatg gcgagaccgg tctggcgggc cagatcgaca ttgggtcgaa
     1321 tgccgatcgc catcaccacc agatcggcat cgatgcggct gccatcggca aaggccactc
     1381 cactgacgcc ttgcagtgga tcgccctcga tgacacgggt gtcggcattc agcatgaagc
     1441 ggatgccctg ctgttcgaga tggccctgca gcagtcgtgc ggccgtttcg tcgagctgtc
     1501 ggttcatcag atgggtgctg ttgtgcacca ccgtcaccgc catgccttgg cgcaacaggc
     1561 catgggcggt ctccaggcca agcagaccgc cgccgatcac caccgcatgg tgccgatctg
     1621 tgcaggcatc gagcaatctg cgggtgtcat cgagcgtacg cagcgcgatg acgccgggca
     1681 gatcatggcc gggaatcggc ggaaccatgg gcctggagcc ggttgccagc accagcttgt
     1741 cgtagtgcag gcaggtgccg cggtcactgc gcacaaggtg cttgatcggg tcgatggtgg
     1801 tgacggtctc acccagatgc agcgtgatct gttgctgctg gtaccagctc cggtcatgga
     1861 acatgatctg cgcctcgctc ttgtcgccgg caagcagcga ggagagcagg atgcggttgt
     1921 aggcgacgtg gggttcggcg ccgaacaggg tgatgtggta gcgatcgcgg tcacgcttga
     1981 gcagctcctc cagcaggcgg ctgccgacca tgccattgcc gatgatgatc agacgttccg
     2041 gttgcatcgt tggtagctcc ccgtcattcc gccgtggtca acgccacttc gacccaaccg
     2101 tcgttgaccc gcaccggata ggtggcgact gccacctcct cctgttcgag acagcggccg
     2161 ctggccaggc tgaagtgctg cttgtagagc ggcgaggcca ccaccagggt gccgcgcagg
     2221 tcgccgacca gcccgcggga gagcacgttg gccttgccga tcgggtcgta gttgtcgagg
     2281 gcatacaggt cgctgccgtc accgacccgg aagagggcga tctgccgttg atcgatcgcg
     2341 gcacagatgc cgctgtcggg cagaatttct tccagtgggc agactggatg ccactgttgg
     2401 gagagggggg tgctcatggt gtggatgctc attggaccag tttgatcctg ggtttgtttt
     2461 cgaggttgcg ctcgcgctcg ctgatcggac gtttctgccc gcgctcctgc acgaagcgga
     2521 tgttgctgtc gccggcatcg ctgttgacga agtggcggaa gcgtttgagt ttggctggat
     2581 cctcgactgc gctcttccat tcacactggt aggtgtcgat gatcagctgc atctgctgct
     2641 ccagctcggc accaatgccg aggctgtcat cgagaatcac tgccctcagg tagtcgatgc
     2701 cgccttcgag gttgtccatc cagaccgagg tgcgctgcaa ccggtcggcg gtgcggatgt
     2761 agaacatcag gaagcggtcg atggcgtgca gcagcgcggc gtcgtccaga tcggtggcga
     2821 acaggtcggc gtgacggggc ttcatgccgc cattgccgca gaggtagagg ctccagccgc
     2881 gttcggtggc gatgacgccg acatccttgc tctgggcctc ggcgcattcg cgggtgcagc
     2941 cggagaccgc catcttcagc ttgtgcggcg cgcgcagccc cttgtagcgg ttctcgacca
     3001 gaatcgccat gccgacgctg tcctgcattc catagcggca ccaggtgctg ccgacgcagg
     3061 atttcaccgt gcgcagcgcc ttgccgtagg catggcccga ttcgaagccg gcttcgatca
     3121 gatctttcca gatcagcggc aactgctcga cacgggcacc gaacaggtcg atgcgctggc
     3181 cgccggtgat cttggtgtag aggtcatact tcattgccac ctgaccgatc gccaccagtt
     3241 tgtccggagt gatctcgcca ccggccaccc gtggcaccac cgagtagctg ccatccttct
     3301 gcatgttggc cagatagtag tcgttagtgt cctgcagcac ggcgtgctgg ccatcgagca
     3361 catggtcgtt ccagcaggag gcgagaatcg acgccaccac cggcttgcag atgtcgcagc
     3421 cgaggccgcg gccatgcttg ccgatcagcg ccctgaacga gcggatctcg ccgacccgca
     3481 ccaggtggta gagctcctgg cgcgagtagg ggaagtgctc gcagaggtcg cgcttgacct
     3541 cgatgccctg cttgaccagt tcggcattga gcacctgggt gaccagcgca ctgcaaccac
     3601 cgcaactggt ggccgccctg gtggtggcct tgagctcggc cagtgtcacc ccgccgccgg
     3661 caatggcacc gcacagcgcg cccttggaga cattgttgca ggagcagatc tgggcgctgt
     3721 cgggcagggc gtcgacgccg aggcccttgg cggcactgcc gtcgtaactg ggcagaatca
     3781 gctggtcggg gttctccggc agcggaatgt cgttcagcac ctgctgcaac agcgttccat
     3841 actcatcgct gttaccgacc agcacgctgc cgagcagctt cttgccatca ccgctgacga
     3901 tgatgcgttt gtagatctcc ttggcttcgt cgatgtaggt gtagctgcgc gcacccgggg
     3961 tgcgggcgtg ggcatcgccg atgctggcga catcgacccc catcagcttg agcttggtgc
     4021 tcatgtcggc gccggtgaag gcgaccacct cgctgccctg caggtggctg gctgcgaccc
     4081 gcgccatctg gtagcccggt gccaccagac cgaagatgcg gccatcccac agcgccacct
     4141 cgccgatggc gtagatattg gggtcggagg tctggcacca gtcgttgatc accacgccgc
     4201 cgcgtggacc gacttcaagg ccggcctggc gtgccagttc gtcgcgcgga cggatgccgg
     4261 cggagaagac gatcaggtcg ctttcgagct cgctgccatc gacgaagcgc atccggtggc
     4321 gcatgccgct gccatcgacg atctcctggg tgttcttctc ggtgtggacg gtcaccccga
     4381 gcgcctcgat cttgccacgc aggatgcggc cgccgccttc atcgacctgc accgccatca
     4441 gccgtggcgc gaactcgacg acatgggtct tgaggccgag atctttcaat gccttggccg
     4501 cttcgaggcc gagcaatccg ccgccgacca ccacgccgct gctgcggcca ctggccccct
     4561 gccggatcgc gtcgagatcc tcgatggtgc gatagacgta gcagccgtca cggtcatggc
     4621 ccggaatcgg tggcacgaag gggtaggagc cggtggccag caccagcttg tcatagtcga
     4681 agcaggcgcc cgcggcactg tgcacctgcc gcttgtcgcg gtcgatggcg acactgcggc
     4741 agttgagctg cagggcaatg ccatgggtgt cgaagaaggt ctgctcgacc agcgccagat
     4801 cggcggcgct gctgccggag aagtaggcgg tgagctgcac ccggtcatag gccgggcggg
     4861 gctcctcgca cagcacggtg atggcgtagc gcgccgtcgc acccgattca gccagctgat
     4921 cgagcagctt ctgcccgacc atgccattgc cgatgacgac gaggttctgt tttttcatgg
     4981 ctctgctccg atggttgtgc aggcgtgatg tgatggaatg gttcgatcaa tgtctggcat
     5041 ggtcacactc ggccagaaac tggatcagcg attcacgcag ttcgtagtag cggggatggc
     5101 tcagcagttc gcgccggctg cgcggccggg gcaggtcgac ttcgagaatc ttgccgacgc
     5161 gggcctcggg gccgttggtc atcatcacca cccggtcgcc gagcagaatg gcttcgtcga
     5221 catcgtgggt gatcatgatg gcggtgagct gggcacggct ccacacctcc atcagcacct
     5281 cctgcagctc ccagcgggtc agcgaatcga gcatgccgaa gggctcatcc agcaacagca
     5341 gcttgggcga gagggcaaag gcgcgagcga tgccgacccg ctgcttcatg ccgccggaaa
     5401 gctcgctggc gcgcttgtgc atcgaattgg ccaatccgac acgggtcagg tagtactcga
     5461 cgatgtcgtc gcgctcattc ggtgtggcat ggggatagac ctgctcgacg ccgagcgcga
     5521 cattgtcgaa ggcgctcatc caggggaaca gactgggggc ctggaacacc accgcgcggt
     5581 cggggccggc gccgtcgatc tccttgccat cgagcacgat gccgccttcg ctgatgtcat
     5641 tgagcccggc cgccatcgac agcacggtcg acttgccgca gccggagtgg ccgatgatgg
     5701 agacgaactc tcctttcttc atcagcaggt cgaacccctt gaccaccgcc accggcccct
     5761 tcggagtcgg gtagatcttg ctgaggttgg cgaattcgag gtaacgcgcc tgaccgaggt
     5821 tgcgtgggct ctctttcagc gtcgacaggt cacgggcaaa gtcgttgctg gtgttggggc
     5881 gcaccggcgg cagctcgatc tcgttgcgac tgccctgttc ctgcttgccc tggccgagcg
     5941 cgagcaggta ctgggtgatt tcaccacgca gcgtcttgaa gcgggcgttg tggttcatct
     6001 cgacgcggtc gcgcggccgc ggcaggtcga cgacgaactc ggggccgaag gtggcgccgg
     6061 ggcccagcgt cagcggaatc acccggtcgg ccatcagcag cccttcgtcg acatcgttgg
     6121 tgatcaggat gacggtcttc ttctcctctt cccagatgcg caggatctcc tcttgcagat
     6181 tgccgcgggt cagggcatcg agcgccgaca gcggttcatc gagcagcagg atgtcggggc
     6241 tggcggccag tgcccgcgcc acggcgaccc gctggcgcat gccgccggag agctcggccg
     6301 gccgtttttc ggtggctgcc gtcaggttga ccagagcgat gtacttgcgg atgtgggcgt
     6361 cgcgttgcgc ctttgaccag ctcctgtgca cctggttgac ggccagcgcg acgttgtcat
     6421 agacgctgag ccacggcatc agcgagtagc tctgaaacac catgccgcgc tcagggccgg
     6481 ggccacggat tttctcgtcg cggaccagtg cctcgccgct gtcggggaag atcagcccgg
     6541 cgatggtgtt gatcagcgtg ctcttgccgc tgccggagaa gccgacgatg gcgacgaact
     6601 ccccctcgtc gatgctgagg ttgatctgtt tgagcacctc gctcttctgg ctgccggcgc
     6661 cgaaactctt gctgatgttg cgcagattga gcagtgacat ggtggttgct tccgctggat
     6721 gtgtcggttc agggggtgtt ctggccgaag gagaacagct tttgcagcgt cagcatcagc
     6781 cgatcgagcg caaagccgat gatgccgatg gtgaagaccg ccaccatgat ccgccccagc
     6841 gattgcgagc tgccgttctg gaactcatcc cagacgaatt ttccaagacc cgggttctgc
     6901 gccagcatct cggcggcgat cagcaccatc cagcccaccc ccagcgacag ccgcaggccg
     6961 gtgaagatca gcggcagcgc cgagggcatg acgatcttgc gcacctgggt gaaccagttc
     7021 aggcgcagca tgcggccgac gttcatcaga tccttgtcga tcgaggccac ccccatcgcg
     7081 gtgttgatca gggttggcca gagcgagcag agggtgacgg tgatcgagga gacgatgaag
     7141 gacttcgaca tccagccgcc cgaggtgttg gtatagagcg cgctgaccac gatggtgacg
     7201 atcggcaacc aggccagtgg cgagaccggt ttgaaaatct ggatcagcag gttgagtgcc
     7261 gagttgatca cccggctgag accgcagagg atgccgatcg gcaccgccag cagggtggcg
     7321 agcagaaaac cggcgaacac cgtcatcagg ctggtcgcga tctggtcgag gtaggtcgcc
     7381 ttgccggtgt aggcacgcag cggggcgggt ttggcctgcg ggttttcggc cagcagtgcc
     7441 ttgttgcgct cgatctggcg ctgcttgaat tcggtttcac gcaggcgctc ggcgcggtgc
     7501 tcgtcgagca gcccggtgaa ctccgtccac acctgcagtg gtccgggaat ggtgccgagg
     7561 ctggtctgaa tccgcgcggc gccgaggtgc cacagcagca ggaagatgcc gagcgccacc
     7621 agcggcacac ccaccatcat ccacagctca cgtgcctgct gccttgggct ttcgcccgcg
     7681 gccagccggg tcatcgggac catgaactgc agaccgatga ggtcgaagaa tctaacgagg
     7741 cgtgccgtca tggttcaaac tcctgccatt ttggacttgg gttgggcaaa ttggctgatt
     7801 tgcttgggtg gatcgattca ttccggaggt cagggtggcg gtcatgccac cccgacgccg
     7861 tacagatcac tgcgcgctct gtgcgccctt gagtccgatc ttgaactgct ccaggtaggc
     7921 gttgggctgc ttgccgtcat agcgcacgcc atcgatgaag tcgctctggg cgggacggta
     7981 gccgtcgctg ctccagggga aatcttcctg cttggcatgg ccctcgctga ccagctcctt
     8041 ggccgccgcc agatagagat cggggcgata gacctgtttg gcggtctgcg cgtaccactc
     8101 atccggtttc tgctcggcaa tctggcccca gcggcgcatc tgggtcaggt accagatcgc
     8161 gtcggagtag aaggggtagg tcgcctggtg ccggaagaag acgttgaagt cgggcacggc
     8221 acgtttgtcg cccttctcat actcgaaggt gccggtcatg ctgttggcga tcaccgctgc
     8281 atccgcaccg acatattccg cacgcgacag catcttcacc gcttcggcgc ggttggcgtt
     8341 gttgttctcg tcgagccaga tggcggcgcg aatcagcgcc ttcagcacct tcttgtgggt
     8401 gaccggattc tgctcggccc aggtcttggt gacgccaaag accttctcag ggttgttctt
     8461 ccagatctcg tagtcggtga tcaccggcac gccgatgccc ttgaacaccg cctgctggtt
     8521 ccagggctca ccgacgcagt agccattgat ggtgccggct tcgagggtga ccggcatctg
     8581 cggcggcggc gtcaccgcca gttgcacctc ggcactggtc agacccgctg ggtcattcgc
     8641 gctgtagtag cccgggtgaa tgccgccggc ggccagccag tagcgcagct cgtagttgtg
     8701 ggtcgacacc gggaacacca tgcccatctt gaacggcttg ccctgggcgc ggaactcggc
     8761 caccaccggt ttcagcgcgt cggccttgat cggatgcagc ggcttgccat cggcgccgag
     8821 cggcagcgcc ggtttcatct tctgccacac ctcgttggag agggtgatgc cattgccatt
     8881 gaggtccatg ctgaaggcgg tgatcacctc ggccttggtg ccgatgccga ccgaggcgcc
     8941 gagcggctgc ccggccagca tgtgggcgcc atcgagctgg ccatcgatca cccggtcgag
     9001 cagcactttc cagttggcct ggggttcgag ggtgacgtag aggccttcat cctcgaaata
     9061 gcgcttctca taggccaccg ccagcggtgc catgtcggtc agcttgatga agccgaactt
     9121 cagctcgctc ttctcggcgg cgggaccggc ggcgcttacc agcgtgaccg gcagcaggcc
     9181 ggctgcactc agtgccaccg tggtgatgat ccgctgccag agtgggcggg atgagaggtg
     9241 attggatcgg gacatcaatg gatctccttg cagggtgatg gaaaacggat gtgtgctgct
     9301 caatgcggga tgtggttgag tgcggccagt gcggccagat cgacccgcag ctgtgccggt
     9361 ggtggctggg tcagcagctg tgcgatcggc tgtgcaggat cgaagcggct gccatcgatc
     9421 agttggtctg gccccatcag cagcggtgtg gaggcttcgc tcagcatcca gtggccggca
     9481 tggctgccct cgctcttgcg atcgatggtc ggaaccggca atcccaattc ggtggcggcg
     9541 gcgcggaaca gatcgggtcg atagacttgc cccgcgagtt cacgcagatc gagtgcctga
     9601 tcgagctgtc cccagcggta catctgcgtc agcagccatt cggcatggct cagccaggga
     9661 aagttggcgg cgtagcggct gaagacgatg aagtcgccgc aattcagggc gctcttctgc
     9721 tgtccggcgc ggaaattgcc ggtcaacgcc atctccagca cttcggccgg ggcatccacg
     9781 tactccgggc gggcgagaag ctgcgccgcc tcgttgcggt ggcgcggacg gtcgagccag
     9841 tcgcaggcca gcagcagcgc cttcagcagc gcatggtggc tggcgggatg ctgctggtgc
     9901 cactgcgcgg tgaccccaaa caccttctcc gggctgttgt tccagatggc atggccgctg
     9961 gtcagcagct ggcccactcc ttcgcagacc gccagtgaac cccagggttc gccgacgcag
    10021 cagccatcga tgctgccatc gcgcagatgc tcgaccatgc gcgccggagg aatcaccagc
    10081 aggctcaggt cgcgatcggg atcgatgccg gccgcggtca gccagtgacg cagctggtag
    10141 tggtggttgg aggtgggaaa gacatgggca aagcgcagcg gtggccgtcc ggcctgccga
    10201 tcgcgctcga tcaactgccg caaccgccag ccatggtcga ccgcatcgct ctgcgacgtc
    10261 gagccatcga cggccagcag tcgctggaac agggtgttcg agacggtgat ggcattgccg
    10321 ttgaggctga ggcagcagcc ggtcagcatc gtcttctgaa tgccgccgag gccgagggtg
    10381 gtggcaatcg gcaagctcga cagcatctgc gcgccatcga gcaggccgaa ggcgaccttg
    10441 tcgcggaggc tggcccagga ggcctcgcgg accagctcga cctcgaggcc aaactcctcg
    10501 aacagaccca gctcatgagc gaccaccagc ggcgcgcagt cggtcagtgg gaggaatccc
    10561 agcttgagtc tgcgtttttc caggtcggtc gacacgttgt tggctctcca gataaaaagt
    10621 ccagaacgaa aaaaggcgcc agcaagacag ccgaacgctg cttgatgacg cctttgtcaa
    10681 ttcttgagct gggtcagacc gccgttggcc tgctggcgct cctgtggtcg atcctgcgtt
    10741 ggatcaatcg gagtgccatc cactctgcca tttcaaaggc aaaacggagg ccaacttagt
    10801 aaaagatggg ttttcagttg aaatgcccgt aaatcaatgc attgccttcc tgaccttgcg
    10861 ggtgtcggcc gaagtggccg cacagcaata tggctgtgca ctgtgcaggt gccgtctttt
    10921 ggtgcatcaa cccagcagtt tggcgatgct gatcaactgg cgcgccacct cgccgatctt
    10981 caatccctgg tccatcgcca gtttgcgcag cgcctggtag gcctcctctt cggccattcc
    11041 tttctgtttc atcagaatgc ccttggcctt ttcgatctcg cgccgttcgg ccagtttgtt
    11101 gcgggtctgt tgcagttcat cgcgcagcgc ctgaaattcg cggaagcggg cgatggccac
    11161 ttcgatgatc gggcgcaatc gatgcggctg catgccgtcg accacatagg cgctgacgcc
    11221 ggcctgcacc gccgcttcga tcagcgcgct gtcattggca tcgctgaaca tcacgatcgg
    11281 tcgcggctgg ttgcaatgca cctcatgcag cgattccagg gtgtcgcggt cgggtgactc
    11341 catgtcgatc agtatcacat cgggctgatg gcgttcgatc tgctcgcgca gcagctggtg
    11401 actgtgcagc agtgccacca cctgatagcc ggccgactcc aacgcctgca gcagggcact
    11461 ggagcgggcc ggcgaggtgt cgatcaccag aatgcgcagt gtcggcttgg ttggagtcat
    11521 gaattgcaaa gtggctgaaa gctgaaagga gcgggcatgc gccagcaagg tcaagcaaca
    11581 atcgtgctct atcgtcggtg attgccgggc cgtttcgcac ccgggtgcgg gcgccgcgca
    11641 gcgtggggga tggtcaggcc gcagtcaagc ctgatatttt gaatggatcg gccggtccgg
    11701 gcagagcgtg ggtcggaagg ggttgccgca gggtcacacg gcttcgtcca gcccgaagtc
    11761 gaaatccatc cggctcatcg gtggtcgcag gtagctgccc tcgatgaagt tgatgccgag
    11821 ctggaacagc atcggcatca cggtggcgct gcgcacggca ggcacgatga tcagcttgcc
    11881 ctggctttgc agctcgcgca cctgtttctg cagccgttcg ctgtcatgca gcagttcggg
    11941 tgcgatctgc acatagtcgg gattgatctg gcgaaaggcg cggaacgggt cttcactgcg
    12001 gccaaactgc cgcagtgaca gggggcagcc gagcgtcttc agccgttcgg cgaaggcgat
    12061 gctctctttc aggtggtggg cggtgtcctg ttcatcgatc tggatcacca ggctgtgggc
    12121 gggcaggtcg gctgcgcgca gggcaacgct gagccaggcg gtcagccctt catcgagcag
    12181 tgcgctggcg ggcaggttga cgaagagcgc tgcctgatgg ccctggtcgc ggtgctccac
    12241 cagtcgcttg atcgcattga gcaggagcca gtggttcagc ttgcgtgcca atccgccggc
    12301 atcggcccag ctccacaggc tgctgcaggg atgctcgacg ccctgctgat cgatcagggt
    12361 caggtggcat tcgtacagtt cggtgccggc accatgcagt ccgacgatcg gttcgaacag
    12421 caggctgagc agatggccgt cgatggcctg ttccagtgtg cgaatcagcg tggtgtggat
    12481 ctcctcgctg tcagtggatc gagccgccgt ttcttcagcg acctccagtg gttcgggtgc
    12541 ggccagtggt ggggcaactt tgggttctac ccagagctgc aggcaggatt cgccctgata
    12601 acgggtgggt gcaaagtgca ggagggcggt gaaatcgcgg ccatcgacgg tttgcagtgt
    12661 cagcgtcagc ggtgggatga cctgttgttc gtcggcctcg ttgagcaggc gcagatgggc
    12721 cttgaacggt tccacgctcc ggggcgcgat caggtcgaga aagggggtgg ccagcagctc
    12781 ttcgtggtca atctggccaa agagtgcgct gtagatcggg ttggcctgca tgtgcatgcc
    12841 atcctgcaga taggccagcg gcagggcggc gaggttggcc agatgttcgg cacggccttc
    12901 ggccacttgc agctgttgcg ccagagactt tgcggcccgc cggtcgagca gttgctgatg
    12961 ctggcgttgc atcagcaggt tcagccgaag gtcatcacca gccggaatca cttcggcaat
    13021 gccctgttgc agcaaggtgg tgatgtgggc cgactcatcg ctggcgctct cgagcagcac
    13081 tggcagatcg atctgtcgac ccgccagcag gccagtcaat gcagcggggt cgacgaatgg
    13141 gcagtgctcg ggggccagca gcagatccca ctcatcgggc tgcatcaatg cgcgctcgag
    13201 ctggagttca tcctcgatgc gctgggcacg cagcggctgc cccgccttgc gcaggcggtt
    13261 gatggccagt tgggcgaagt catcctcgcc atccagcacc aacagccgca aaagggtgcg
    13321 ttcaaccatg ccgcgtgacc gtcatgaccg gcgcgcacag ctggagagag ggtggcgcag
    13381 cttcgcgcgc cggatcagag aatctgccat agctggtcga aaccggatgc agggattggc
    13441 agattccgct cgattttttc cgtgttctcg gtgcgtgcgc aggcttgcag cgccaggaat
    13501 tcgaactggg cgaaggtgtg gtttttacgc aattggcgca ccagcttgat gctgtttctt
    13561 tggccgggct ggatgagtgt gactttgtcc ttgctgcgaa atggcttgca gaacagcaac
    13621 aatgaggccg cttggccggt ggtcggcagc gttggcagca gcaggccgcg catgaattcg
    13681 gtgacctccc cccgtttgcc ctccaggcgc accgcacagg cggtcgcatt gggccccagc
    13741 agttcgacgc cgacatgggc ctgactgcgc tcatcgagct gagtccaccg cacggtggcg
    13801 atgctccagg gcagtgatgg ctcggtgcga atgccaagca gggtaccgtt ctggatgcgc
    13861 tccggaatcg actggtgcca acgcaggcgg tagccattga cactgctgtt ggcgacctgc
    13921 acctgatgga tcgggtagct gggctggtgt gggagcgtct gctcggcagc cgcagtcacg
    13981 ggacgcgtga actccagcac tggcggcaac tgcagatgga tcagcggatt gaacttctct
    14041 ctggcatcaa aggcaccatc ccagacatcc gcggatggtg cagcgaaggg ggagcctttc
    14101 tcaggaggca attgcgtcag ctccagccgc ggcagcaggc ggttgagcca ttcgctgaaa
    14161 tcgagccggt tggcgcagtg aaaatggatg ctcggcaggc caatgatgat ctcgacctgc
    14221 tcatggctct cgatgcgttg aaaccggcgg gttgcccgcg caccccaact gacaaacagt
    14281 tgatcgacaa gctcgcgctc gatcgggcca ggctgatcgg ttggtgtcgc agcgatctgt
    14341 tcgagtcgct ccagaatcgg tttgaggtcg agcagtcgcc atgtgccggg cgtaatcggc
    14401 cgctcatggt cggtgtagat tgccggcagg tcgctctgca gatcgacccg gaagtggtgg
    14461 ctggcaggcg tctgttgcag tggcagcaag cgcgcctcgc ggctccacag ttcgagcgcg
    14521 cggtacagtg gcaggcaact ctgctggcgc atctgctgag gttgggcgat gtcgaacagc
    14581 agcacacgca ggtaggcggc ctcgatcgac aggccactgc gctgttgcag cagtgggtca
    14641 ggcacctcca cggccgtgca gtcgagccgt tcggccagca gatggagttg atgcagcgcc
    14701 tgccagaaac cggctggcgt cggggcatag agttgactgc tgcgcagcag aatctcgctt
    14761 tgggcggcga tggcgcgcgc gatggcgctc accaccaacg ccggaacagc ggccgatctg
    14821 cccggggtga gcagcccctc gttgtcggca tcgaccagca cctgccggta accgcgccac
    14881 aacagcgctt gcatcgcctg cgccaatcgg gcggtctcca gtgcggcgtt gcgcagctgc
    14941 agcggctgat tgctgaagtg ccgttccagc agcatgctca gctgttgggt cggtggccgc
    15001 agcagctcca gcagtgccag ccggacggcc gccgtggtca ccagctggtt gagctcagtc
    15061 aaggcattga aaacttgccg cgcactggtg ccgacactgg tgaggggcag atgggcaatc
    15121 caatcagcga tggccgcctc attggccgca cagaatgaga gccgctcgcg tgagggcttg
    15181 ccgacagtaa agtgaacctg gtcgttgatg tggtgcttcg agtccaaaat cgggcgctca
    15241 ctgaaggggg acagccggtc gaaccgcctg cggtgggagt tgctgtttca agtataggcc
    15301 gctttgtgca gcgcgccgga cgcggccgtg ccgattctca tcagtggcag cgtgccgagt
    15361 gagctgttgt catcgctcaa ctgcccagtg ggcgcttgct gcgctctccc ggcacctcga
    15421 ccaccagccg tggtgccaga tagccactca cccgcgccag cagtgactgg tggatctgcc
    15481 gggcctcggg ttccggcacg gcaaaatggg ccgcgccggc caccggatcg agcagatgga
    15541 ggtagtaggg cagaatgcct gccacgaaca gcttctcgct cagctcggcc agggttccgg
    15601 catcgtcgtt caccccgcgc agcagcacgc tctggttgag cagggtgatg ccggcagcac
    15661 gcatccgctg cgccgcgctg accacgtcac cgtcgatctc gttggggtgg ttgctgtgca
    15721 gcaccatcac cgtggtcagg cggctgccgg tgagaatatc cagcaattcg gcggtgatcc
    15781 gctgcggaat caccaccggc agccgactgt gcagccgcaa tcggagtagc tggggcaggc
    15841 gggcgagctg ttcgatccag cgctccagca cggtgtcact cagcgacagc ggatcgccgc
    15901 cactcagaat cacttcggag atctcggggt ggtgagccag atactccagc accgcctgct
    15961 ggccggtgcg tccctgctgg tgtgcctgat agggaaagtg gcggcgaaag cagtagcggc
    16021 aatgaaccgc acaggcgccg gtggtgacga tcagcagccg cgaacggtac ttgtgcaaca
    16081 caccgggcag cggtgagtgg tgcgcttcgt cgagcggatc gctggtgaaa ccggggcgtg
    16141 ccagcagttc ctcggccacc ggcagcacct gtcgcagcag cggatcatcc gggttgcccg
    16201 gctcgatgcg ggccaggtag ggcgcgggca cccgcagggt gaactgccgg gcagtggtgg
    16261 catcgccggc caactgtgcc ggatcgagct tcaaccgccg cgccagttcg ggcagcgaca
    16321 gcggggcttc gcgcagcagt tgctgccagg atgggccgtg ccaacagtgg gtcgatagcg
    16381 gtatgattgg cggctttgaa tcaggcaagg gggtcaatga tctccggctg tggtcgcgca
    16441 tcgactgccc gcagtccgac acatcatcaa tagaggagct tatggcgaac tattctacca
    16501 atgaatttcg tgccggcatc aaggtgatgc tcgatggcga tccctgcgcg atcgtcgaga
    16561 acgagttcgt caaaccgggc aaggggcagg ccttcaaccg ggtcagactg cgcaacctca
    16621 agaccggtcg ggtctgggag cgcaccttca aatcgggcga gacgctcgag gcagcggatg
    16681 tgaccgaccg caacctgcaa tacctctaca ccgatggtga gcagtggcac ttcatggaca
    16741 acgacagctt cgagcagtac gcggtcgaca agcgcaccgt tggcgatgcc gtgcagtggc
    16801 tgcgtgagca ggatgtctgc atcgtcacgc tgtggaacaa cgccccgctg gcagtcactc
    16861 cacccaattt cgtcgaactc gagatcgtcg aaaccgaccc cggcatccgt ggcgacaccg
    16921 ccaccggtgg caccaagccg gcccggctca gcaccggcgc cgtggtcaag gtgccgctgt
    16981 tcgtcaatca gggcgaggtg atccgcgtcg acacccgcac tggcgactac cagagccggg
    17041 tcaaggagtg agtcaggcac ccgctgccgg tggcgactgg cagccgagtg ccagcctcgc
    17101 cgcactgcgg cagtgggccg agttgttgag ccgggtgcgc gccttctttg cccggcgcgc
    17161 gctgctcgaa gtgaccacgc cgctgctctg caccaccacc gccaccgacc cgcagcttgt
    17221 cagtttcgtc gtgaccgagg gcgagccgct cccgcgcttt ctgcaaacct cgccggaact
    17281 ggcgatgaaa cgactgctgg cggcgggcag tggccccatc taccagatcg ccaaggcttt
    17341 ccgccagcat gagcgcggtg cccgccacaa ccccgagttt catctgctgg agtggtaccg
    17401 gcccggcttt tcactcgaac ggctgatgga tgaggtggcc gaactggtgg ccgagctgac
    17461 cggccgtcac cacttcgaac ggctcagcta ccgcacactg tttgctcgcc acctcgatct
    17521 cgaccccttc gtcaccccga ccgaagaact ggaacatacc gcccgccgcc agatcgacct
    17581 gggccccacc gtggcgggcg accggcagtt ctggctcgac ctgctgctga cccatctgat
    17641 cgaaccgcgg ctggcgtcga ttccggccct cttcatccat gactacccgc cagagcaggc
    17701 cgcactggcg cggttgcggc gcgatgagtg tggcgtctgg gtcgcggccc gcttcgagct
    17761 ctacctcgat ggcatcgaga tcgccaacgg ctacgatgag ctgaccgatg ccgatgaaca
    17821 gcgtcgccgc ttcatcgccg acaaccagcg ccgtcagcag cagggccagc cgcaactgcc
    17881 ggtcgatgag cggctgctgg cggcgcttgc cgctggcctg ccagcgactg cgggggtggc
    17941 gctcgggctg gagcggctgg cgatggcggc gaccggtcag cgcaggatcg acgaagtgct
    18001 cgccttcacc ttcgagcggg cctgactgcc gccagagttc aacccttgaa ctgccccagt
    18061 gcctgcccca tgcgcactgg ctgctgtgtg aggttggcat tccagcgcat gcgcccgggt
    18121 ggcaacagca ggatcaccgt cgacccgagc cggaagcgac ccaactcatc gccccgcttc
    18181 aacgccaccg gcgtgccacc ctgccagcgg ctgatgccgc gcccgggagg tgtcaccacc
    18241 ccggcccaac cggtttcgat gctgccgacg atcagcgcgc cgaccagaat caccgccaac
    18301 ggcccctcgt cggtgtcgaa gtggcagatc acccgctcgt tgcgggcaaa cagatcctcg
    18361 acccgctcgg ccgtgagctg attgaccgaa aagagccgtc ccggcacatg caccatgctg
    18421 gtcagacgac catcgaccgg catgtgcacc cggtgatagt cgcgtggcga gaggtagagc
    18481 gtgcagtagt cgccatcgcg gaactgctcc accgcctgct gatcgcccgc caacaacgcc
    18541 tgcaaggtgt agtgacgccc cttggcctgc accagccgct gtccgagcag cggaccatgc
    18601 tcggtgatct ggccatcggc cggggagacc aggctgtggg gctcggcagc gatcggtcgc
    18661 gcgcccgcac gcagcggccg ggtgaaaaag gcattgaagg aggcgtaacc ggtcggtgtc
    18721 gacggctccg cctcggccag atcgacctga tagcgccgca cgaacagccg aatcaaccac
    18781 tgggtcagcg cgcccagtgg ccggtcggcc aacagcccca tcagccggct cagcaacttc
    18841 tgcggcagca gatgttgcag cagaatgaac agcgaatcac gcatgcaatg ctccgtaagc
    18901 tccaacaaga gtcgagggcg gggctgttgg cgccccgagt ggccgccatt atagggaaat
    18961 gaaaaatgaa taaaaaaatg gggccggttt gagctgttcc gcagtgttgg tcattctgga
    19021 gaatggcccg tttcaaagag tgccgccacc atccagagtg cggtcgatga ctcggcggcc
    19081 acctgctcgc ggcatatcag catcccaagt cggcgggttt tcaggtgacg actcgttgcc
    19141 ggcaccggca ggcgtccgat cgatgaccgg acgcatccag catgacgacc cggcacaacc
    19201 cttgctggcg cccagtgcga ccaattcgaa agcgtctgcc gacgccccga gccgaagaga
    19261 ccacggcgcc ggcgctgcct aaacggaggt ttgaacgatg aagctgcgca ccctgctgct
    19321 cgatctgggt ggcgtcttct accagggcga ccacctcatc ccgggggcgc tcgaggcact
    19381 ggcccggctg cgcgccagcg gtctggcgct gcgcttcgtc accaacacca cgcgccagag
    19441 ccgcgccgcc ctgcagggcg aactggccgg tcgcggcatc gccattgccg aggatgaact
    19501 cctcacgccg gccgccgccg cccgggacac gctgcttgca cgccgcctgc gcccgctgct
    19561 gctgatccac cccgacctgg cgcccgactt cgccggtctc gatacggctg aacccaacgc
    19621 cgtggtggtg ggcgatgccg gcgagcactt cagttatacc gggttgaacc aggcctttcg
    19681 gctgctccag gccggcgcgc cgctgctggc gctcggccgc aaccgctact ttcaggagcg
    19741 cgacggcctg tcgctggatg ccggtccctt cgtcgccgcg ctcgagtatg ccgccggcgt
    19801 gcaggccgaa ctgctcggca agccggcgcc ggctctgttc gccgctgcgc tggagagtgc
    19861 gggctgtcca ccgacggcgg ccgcgatgat cggcgacgat gtggaagcgg acatcaacgg
    19921 cgccctggcc gctggcctgc gcggcgttct ggtcagaacc ggcaagtacc ggcccggtga
    19981 cgaagcgcat ctggctcccg gcggcgaact ggcggacgac gtcggcaccg cagcgacgct
    20041 gctgggcatt gcctgaagcg cgggtcaagc aggcgtagtg cgcgcaaccc caggcgcgcg
    20101 aacgggaagc cgccgatttt cctgatcagg aacaggaaac cgtggtctgt ccccaattag
    20161 gtgtccccaa ttatgccgga cttgcgcggc tttatgcgca aggtccggtt gaccgaaggg
    20221 ttaggcatca ccgcgccatg gagaacttcc ttaacgttca aggccgtgtt ctcggacgca
    20281 actcagatag taggcttcct tgttggcttg actgtacaaa ccctctggta agcccttggg
    20341 agcctgcttg cccataattt gcctaagaaa ttccgcagcc tgctcggttg actgcgtctc
    20401 gcaagccgcc cgaatttgtg acgggcggta ttcataccaa taaaacgcaa gccccatgcc
    20461 gacgattagc atcacagcgc caagcgctac gatcgacctg ttgtccatga accccccttt
    20521 ctcaattgtt gctcatcaca tctcggccac tgacgttgcc acgaaagggc aagttcccgc
    20581 cgccgacgcg agaactgttt ctgatgccta acgcaaagct aacctgcgag ctacgaggcg
    20641 cgaagcaccg acgtagcgag tcaggttgag cgtcgtgtta ggcctgtgct gcactgggac
    20701 gcccccgccg ccgaattgcc tccagcattt ttcgttcgac ctctgtcttc tctgcggaga
    20761 agtgtgcctt tcgatgacac aatgggcaca cagcaacgat attatccggg ctatcaggac
    20821 cacgctcaga aacaccaaca acatgatgtg tttctagata tctctcaccg ctctcagtaa
    20881 gaaacccaag ctctccacag cactcacaac ggccccttgc aattttgaga acctgctcgc
    20941 gaactcgcgg gtcccggcga taaacgttgc cgcttcgagc gcgcaaaacg acttgcgttg
    21001 cgccggttga ttgctcacgg tgcggggccg acaaaatgtc ttcgccaatt gcgcaggcag
    21061 cctttgctgc ttctacaaac tcgggagcgc gcgcctcaag gattagcgct gcactgtgag
    21121 cacggtagaa gacgctgttg atttgagtgg attgagttgc ggccagagtt ggtacttcgc
    21181 ttatggaata caaccgagca aaagacaata cgcccgaccg gtcgttgcct acgatagcta
    21241 aaaagtgtgt tgctcctcgt ttggcggcgt cttcgcaggc taccgagatt gcagacaagc
    21301 catcaggtga aacagaaagt tttttccaag gaaaacgcac ggcctccgct agacccggcc
    21361 attgtgtggc gcttttgatc cagatgttta caggttctcc attaggagcg attgctgaca
    21421 cataacgccg ccgtgctttc ttgccgccaa tatcgaattc cagcgtcgaa tagccgaaag
    21481 cgcctagcgc acgaaccgcg gcgttgaata gaaccgcttc ccgttcatgg cctctgtgtt
    21541 ctggcgtttc agatagatcg tgcatagaga acaggcctaa cgtaaattag acgaacccaa
    21601 acatgttgat agatgtcgcc caaatcaagc tctgcccctg agtttcaagc ggttacgccg
    21661 atcatgccga tttgggcgaa ccaaattttt ccggatgacc gatcccgctt ttcccaagcc
    21721 gccgaagctg ttcgatcctc cgatcggagc cttgcgcgcg acattgcggc cagtccgcgt
    21781 cgatcgagca cggcatcgtc gcctcccact aagccgacag cagccgcgcg acgatggcgg
    21841 gcgcttcggc cagtgcggcg tcgagcgcat cgggctggtc gctgccgccc tgtgccatgt
    21901 cggcgcggcc gccgccgcgg ccacccatca gggcggtgat ggccttgatc agctcgccgg
    21961 cggcgatgcg ggcagtgagg tcggcggtga cgccggcaat caggttgacc ttgccggcgc
    22021 tgaccgaggc gagcaggatc acgccgctgc cgagtttgtc gcgcaccttg tcgagcaggt
    22081 cgcgcagccc tttggcctcc atgccatcga cggcggccac cagcagcggt acatcctgaa
    22141 cctgaaccag ccggtcgagc aggtcggcgc tggcggcact ggccagtcgg gcgccgagct
    22201 gttgcacgat gcgctccagc cggcgctgtt cgtcgagcag ggcggtgagc ttctcgggca
    22261 actggtcgat tggtgcgcgc aactgctggg ccagttcgcg cagcaggttg gcttcgtact
    22321 gggccagttc gagcgcggcg gcgccactca ctgcttcgat gcggcgcacg ccggccgaga
    22381 tgccgctttc ggcggtgatg cggaacaggc cgatgtcgcc ggtgcggcgg gcatgggtgc
    22441 cgccgcagag ttcgaccgaa aagccgtcgc ccatggtgag cacccgcacc tcggcgccgt
    22501 acttctcgcc gaacagcgcc atcgcaccgc gcgccttggc cacatcgatc ggcagcagtt
    22561 cgacgctgat cggactgttg cgacgaatct cgtcattgac ccgctgttcg atctggcgca
    22621 gcgtcgaggg cgcgatggcc tcgaagtggg cgaagtcgaa gcgcagccgc tgcgcatcga
    22681 ccagtgagcc cttctgctcg acatggctgc cgagtatctc gcgcagggcc gcatgcagca
    22741 ggtgggtggc ggagtggttg agggcagtgg cctggcgccg tgccgggtcg acctcggcgg
    22801 tgagtctgct gcctgcttcg aggttgccct gcagcagccg cacctggtgc aggtggttgt
    22861 tgccctgctt gagggtgttg agcacttcgg cccggccgcc gccggcacgc aactggccac
    22921 ggtcgccgac ctggccgccc gcttcggcgt agaacggggt gcgatcgagc agcacccagc
    22981 cggtttcgcc gtggctgagc cgttcgaccg gttcatggtc gcggtagagg gccaccactt
    23041 cggcatcgtc tgcggtctgt tcatagcccg tgaacaggca ttcatggtcg ctctggcgaa
    23101 tcagcgcggt gctgtcgagg gcgaattggc tggcggcgcg ggcacgctca cgctgcgcct
    23161 gcatggcggc ttcgaagccg gcggagtcga tctgcaggcc gcgttcgcgg gcgatgtcgg
    23221 cggtcaggtc gagcggaaag ccataggtgt cgtagagctg gaacagcgtc tcgccgccga
    23281 tgcaggctcc gttcagttgg gcgatcgctt cgtcgaggat gcgcatgccc tggtcgaggg
    23341 tgcggctgaa ctgctcctct tcggccagca gaatccgttc gatctgcgcc tgcctggccg
    23401 gcagctccgg ataggccgcc cccatctgct gcgccagcgc ggccaccagc cggtggaaga
    23461 agggctgttt gacgcccagt ttgtggccgt gtcgcaacgc acggcggatg atgcggcgca
    23521 gcacgtagcc acgcccttcg ttgccgggaa tcacaccatc ggcaatcaga aagctgctgg
    23581 agcggatgtg gtcgacgatc accttcagtg agtcgttggt gtgggcatcc tgccggccgg
    23641 tgatgcgggc cgccgcttcg agcaggtggc ggaacaggtc gatctggtag ttgctgtgca
    23701 cgccctgcat caccgcggcg atgcgctcca gccccatgcc ggtgtcgact gagggcttgg
    23761 gcagcggctc cagccggcca tcgggcaggc ggttgaactg catgaagacc agattccaga
    23821 tctcgatgta ccggtcgcca tcttcatcgg gcgagccggg tgggccgccg gccacctcgg
    23881 agccatggtc gtagaagatc tcggtgcagg gtccacaggg gccggtctcg cccatcgacc
    23941 agaagtttga agcgtcgcca agccgtgaaa accgttgtgg atcgatgccg atctcatcga
    24001 gccagatgcg ctcggcctcc tggtcttcgt ggaagatggt gacccacagc cgcgccttgg
    24061 gcaggccgag acgctcggtc agaaagcgcc aggcgtagtg gatcgcctcg cgcttgaagt
    24121 agtcgccaaa gctgaagttg cccagcatct cgaagaaggt gtggtggcgg gcggtgtagc
    24181 cgacattctc cagatcgttg tgcttgccgc cggcacggac acagcgctgt gacgaggcgg
    24241 cgcgacggta ggggcgcaca tcctgtccca gaaaaacatc cttgaactgc accatgccgg
    24301 cgttggtgaa gagcagggtg gggtcgttgc ccggcaccag tgaactgctc ggcacgactt
    24361 cgtgcccctg ttcggcaaag aaatcgagaa aggcgcggcg aagttcagag ctgttcatgg
    24421 tctgcctgtt ggagtgaaaa ggggtgccga ggccgggccg gcggcaaaat tatatgccga
    24481 atcaatcttc ttcagcttca gtgtcatgca gtgcgtggcg aatctgtcgc tgggtgaaac
    24541 cacgcgcctg cagaaagcgc atccatttgg cctgggtcgc cgggtcacgc gggcggcccg
    24601 ggccaaaacg tttctgtttg gcatggcgcg cggcgctcag ccagagttcg gactccgcat
    24661 cgatgacctc agtgatcaac tgccgtgaca ggccgcgcgc cagcaggtcg ctgctgacat
    24721 gcagtggacc aaaaccggct tcgcggcgct ggcgacagcg catttcggca aaacgggcat
    24781 cactcagcag cccctcctgt tgcagttggg tcagcaccgt ttcgatttcc gttcggtcgc
    24841 cccctttggc gagcagcttg gtcgccagct cgtgactcgc atgttcccgg cgcgccagca
    24901 aatcgagcgc ctggtggcga agcgggcggg tcttggcagg ttgtggtggg tctgaaggca
    24961 tggacacggt gtgcttcaca gcagcagcgg ggcatcgggc agttcattcg ggttggcctg
    25021 atcctgcgcc ggaaaatgca gggccaactg ctgggcaatc gcctcgatgc cggcaatgct
    25081 gccctccaga taggcgccac gcccgaatgc ctgctccatc atccggcaga tctgcgccca
    25141 gcccgcgctg ccgacccggc ggtgaatgcc acggtcggcg atgatctcga catcgcgctc
    25201 tgccagcagc aggtagatca gcacgccgtt gttctgttcg gtgtcccaga ttcgcaactg
    25261 tgagaagagc tccagcgccc gatccgctgc gctggtgccg gcccacagcg gcccgatgtc
    25321 gagcgcggcc tcgaccacga accgcagttc gccgctgtac gaggcttcgc tgcgctggat
    25381 cgcctgctcg atctgccgca gcgcgctggc cggaaaggcg cggcgcaggg gtcgctcgcc
    25441 gctccacaga tgggtcagga tgcgcttgag tttgatggtc accatcgtcc ggatgcacct
    25501 ccgccgccaa aaccgccgcc accgccactg aaaccaccgc cgaagccgcc accgccaaaa
    25561 ccgccgcgcc ctccgaaacc gccgggaaaa ccgccgaggc cacggcctgc atggccgagc
    25621 agggtgaaga gaaaggcgcc gatgccggca agcagcgcaa tcagcagcgc accggccaga
    25681 aaccaggtga gcaggccgat caacgtgccg ctcaccagcg cgccgagtgg tcgtcccagc
    25741 aaggcgcgca gtgcgccgcc ggccatcagc acgatgatga aaagcaacgg cagcaggttc
    25801 gcaatttcca gacggtcgcg ggcagggcgc tgggaagggg gcggcagggg ttcgccatcg
    25861 atcaaccgga tgatctgctc gatgccggcg gtgatgccgc cgtaaaaatc accctggcgc
    25921 aagcgcggcg tgatcagctc gctgatgatc cgtttgctga gggcatcgtt cagcacccct
    25981 tcgagcccat agcccacctc gatgcgcagg gtccggtcat ccttggcgat gatcagcagc
    26041 acgccgtcgt cgatcttctc ccgtcccagc ttccactgct ccaccacccg catcgagtac
    26101 tgctcgatcg actcgggctg ggtggtggcg accagcagca gcgcgatctg gctccccttg
    26161 cgctgctcga agctgcgcag tctctgttcg atgttgcgct gctgttccgg ggtcagggtg
    26221 gcggtctggt cgatgaccgg cgcagtcagg ggcggtaccg gcagctcggc atggctgatc
    26281 cagacgatgc cgagcagtgc tgtcagcaac agcccggcca tgctttttgg caggccgagc
    26341 cggttcatcg ggatttgctg tcgggcgccg gggcaaagtc gaccttgggc gcggtggaga
    26401 tctgctgttc gttctccacg ctgaagttgg gcttcacgtg gtagccgagc agcatcgcgg
    26461 tgaggttgga ggggagtgag cgcacggtga cgttgtacgc ctgcaccgcc ttgatgtagc
    26521 ggttgcgcgc cacggtgatg cggttctcgg tcccttcgag ctgggcttgc aggtcgcgga
    26581 agttggcgtc ggatttcagt tgcgggtagt tctccgccac caccagcagg cgcgacaggc
    26641 tgctggtgag ttggccctgc gcggcctgaa agcgggcgaa ggccgccgga tcattcagca
    26701 gttccggggt gagctgaatc gagccaacgc tggcacgggc ctgggtcacc tcggtcagca
    26761 ctgctttttc ctgtgcggca tagcccttca cgctgttgac caggttgggc accagatcgg
    26821 cgcggcgctg gtactggttc aacacctccg accaggccgc cttgatctgc tcatcctggc
    26881 tctgcagggt gttgtagccg cagccactca gaatcaacgt caacagggcg atcagccaga
    26941 ttcgcattgg atgcatcggt cactcctcca tctgtttttc agacattgtg cgtgacttca
    27001 cgtttttgtg gttgttcttt ctgacgcgtc atggctgccg gtctaagctt gaactcatgc
    27061 atccctgcaa acaagcccaa cccaggaggc gatcgaccat gtccatgcct gtaacatccg
    27121 acggtaactg ttcatattct gccagcagga ccttgttctc tcgcggtttt tgtctgttgt
    27181 tgacgctggc cagcctgctg ctgccaacgg caagctgggc agcacgctat caggcggtgg
    27241 ttcagtcacc caacagcagc ggcacggccg gcgaggtgcg caatgtggtg ctggcctttg
    27301 atgaccagtg gaactatctg accgccgact tcaccattct gccgagcgaa aatggcggtg
    27361 tggccaatgg tttctggatg gtcgtgagtc cggccaatgc gaccggcaat gccgcgcgct
    27421 cgctgaccct ctatgccgat ctgaacaccc atcaggtgag cctctatcga ctgagcggtg
    27481 atccgctcaa ctttgccgtc gaccgcaaca aggattttct gtcgagccac gccaatgccg
    27541 tgcgcgtgag cgatgaggag acgtcgggcc gccgggtggt tcgcattgcg gtgccgctga
    27601 tggtcgagaa cggcctgggg cgctttggcg ccggcggggt gagctggcag atgcagcttc
    27661 tgctcgatgc ccgcttcagt tacgacgcca atcaccatct gctgggcgtg aaatcgagcg
    27721 cccagagcga cagccaggtg caggcgcaac cggtcgcacg gctcaactcg ccagccgagg
    27781 cgccgcaact ggccggcagc aaagagagcc cgctgtggat gctgctgttc gtctttggcc
    27841 tgggcgggct gatctgggtc tggcgccatc cacgctaccg tcgccatgtg cggcagagcg
    27901 agcagcgcgc cgccgcagcc gctgccgcag cgaaagtcac cacagcgtca gttgacgcgg
    27961 ttgagagtga tcggctgatt gcagccccga aaccgtgatt cccagcagcc gcactggccg
    28021 aaaatcggtc agtgcgcgct gcaacagcgt cttcaacact gaatcgacct gcggtgccga
    28081 ggcaaaaccc tgtcgcagcg tcatgccgcg ggtgaccgag cggaagtcac tgaacctgac
    28141 cttgacggtc aacgtctgcg cccgctgccc ggtttgctgc aactcctgag tgacgcgatc
    28201 gagcagccgc tcgagttcgg acaccatctg cgctgaatcg gtcagatcat cggcaaaggt
    28261 ggtctcagcg ccgatcgatt ggcggatgcg tgaagggttg accgggcgcg gatcgatgcc
    28321 ggcagccatg ccatgatagt ggcgcgccag tttgccgaag tgctgctcaa gctcgggcag
    28381 gccaaagcgc agcagatcgg cgccaaactc gatgccgagc gatctcatcc ggctggcggt
    28441 gctgcggccg atgccatgga acctgtcgat cggcaaggcg gctgcatggg ccgccccctc
    28501 gctgggcatg atgacgaaga ggccgtctgg ttttccccgc tcacaggcca gtttggccag
    28561 aaacttgttg tacgagacgc cagccgaggc ggtgagctgg gtcttctgcc ggattctctg
    28621 cctgatctcc tgcgcgatca gactcgccga gccccgatgc agggtgctgc cggtgacatc
    28681 cagataggcc tcatccaacg acagcggctc caccagatcg gtatagtgct gaaaaatctc
    28741 gcgaatctgc agtgaaacct gttgatagat ctcgaagcgg ggcttgacga acagtgcctg
    28801 cggacagagc tgataggcgc gcgccgatgg catcgccgaa tggatgccgt aacggcgggc
    28861 ttcataactg caggtggcta ccaccccgcg cgaattgggc gcgccaccca cgatcagcgg
    28921 cttgccgcgc cactgcggat gatcgcgctg ctcgatggca gcaaaatagg catccatgtc
    28981 gatgtgaatg atctttcttg tcgattcatt gatctgcgac atggcccgac cagtctttca
    29041 acccgaaact ggccacggca ttgaagaggg gagggggcag gcccgggtcc cacgggagca
    29101 ggatggattt gacattgcaa tgagaaacgt tatcattaac aacaccagca gattgcaacg
    29161 cagcgtacca ggtggtgaca tgaacagccc tcaactcgcg atgcagtacc aggcggctgg
    29221 ggcggcggac ccggtgtgga ttgccagcca gccggaccgg ctcatgctca gtgaggtgcg
    29281 cgcgaccgag caggtgacac tgctgcagat tgccggcggc aaccaggtca cgctgcgtct
    29341 tctgagcatg ggcattcgcc ccgggcagca gattcaggtg ctgcacaacc gcacgggtgc
    29401 cgtggtgatc gcagcaggat cgggccgcat cgcggtcggc aagagattgg cgcagcagat
    29461 caccgtgcag cgcggctgaa cggccggccc atgcgccaat ataccattgc ggtggttggc
    29521 aaccccaact gcggaaagac caccctcttc aatgccctga ccggggcccg ccagcgaacc
    29581 ggcaactggc ccggtgtcac cgtcgatcgc aaggaagggc agttcgagat cgatggacag
    29641 ccgatccgat tgatcgatct gcccggtgtc tacagtctcg accgcttcag ccaggcactg
    29701 gatgagaagg ttgcctgcga ctacctgctt gccggcgaag ccgacctgct gatcaacatc
    29761 ctcgacgctg ccaatctgga gcgcagcctc tacctcaccg cacaactgat cgaaatgggt
    29821 gccccgctgc tggtggtgct caacatgatg gaccatgcga cggcgcaggg actgactgtc
    29881 gacccgttgc aactcagcca gcaactgggt tgtccggtgg tggtgacggt ggcgagtcgc
    29941 gagcagggca tcgcgcaatt gaagacggcg atcgccgaac gactgactca ccccacgacg
    30001 cggggtgatg gcattctgca tccggtgcag atcgagttcg ccgtcgccac cctggtggat
    30061 cggctcgatc cgctgggccg cgatccagcg cggcgctggc tcgcgctcga actgctggcg
    30121 aatgagggtg tcgcgcctgc ggtcgagcca gaacttctgc atgacgtcgc cgagatgcgg
    30181 cgccagatca gcaccgaact gcacgaagat gtcgaattgc tgatcgccga cagccgctac
    30241 ggttttgccc ggcgggtcgc acaggaggtg gccacgcgcg gtcaggcacc aggcagcact
    30301 tcttcggatc gaatcgatcg gatcgtgctc aaccgttttg ccggtgtgcc gatctttctg
    30361 ggcgtcatct atttgatgtt cctgttttcg atcaacttca gcggcgcgct gatcgacttt
    30421 ttcgatcagg tgacaggtgc gttgctggtc gatgggctgg gtcaactgct gttggcgctc
    30481 ggcctgccgc agtgggtcaa ggtgctgctg gcggacggtc tgggtggtgg agtgcaggtg
    30541 gtggcgacct tcattccagt gatcggcgca ctctacctgt tcctgtcgtt tctcgaggat
    30601 tccggctaca tggcgcgcgc cgccttcgtg atggaccggc tgatgaaccg gatcggtctg
    30661 cccggcaagg cctttgtgcc actgatcatc ggctttggct gcaatgtgcc cgccatcatg
    30721 gcgacccgca cgctggagtc acatcgagaa cgcattctca cctcgatgat ggcgcccttc
    30781 atgtcctgcg gcgcgcggct gtcggtctat gcactctttg ccgcagcgtt ctttccggtg
    30841 ggtggtgcca atgtggtgtt cagcctctac ctgctgggca tcctgatcgc cgtgctgact
    30901 gcgctgctgc tgaaaaacag cctgttgaaa ggcgaggccg atgcctacct gctggagttg
    30961 ccggcctacc acctgcccac gctgaaaggc atctgcattc acacctggga caaactgaag
    31021 ggcttcgtgg tccgcgccgg ccgcatcatc atcgtcatgg tgctggtgat caatctgctc
    31081 aactcgatcg gccgcgatgg cagcttcggc aacgaaaaca gcgaaaattc gctgttgagc
    31141 tggattgcac gcgaaggcac gccgctgttc gcgccgatgg gcctgcgcga ggagaactgg
    31201 ccggccgtcg tcggcatcct ggcgggtgtt ctggccaagg aggtggtggt cggcacgctc
    31261 gactccatct acagccggat ggaccgcgaa tccgccggcg aagccgacgc cgcaacaccc
    31321 ttcgacctgc tggcgcaact gagcgcggca gcggccacga ttccgcagaa tctgctgcaa
    31381 ctgctcgatc gactgggtga tccactcggc ttcagtgtca tcaaggaggg ggatccgagc
    31441 gcggcggcgg ccgagcagca ggtgagtctt tcaaccttcg gcgcgatggt cagccgcttc
    31501 gatgggcagg ccggagccta tgcctacctg ctgttcatcc tgctctactt tccttgcgtg
    31561 tcgaccatcg ccgcgttgcg acgggagacc ggcactggct ggaccctgtt tgccgccggc
    31621 tggggcactt tcatcgccta caccagtgcg accttctgct atcaattggc cagctttgcc
    31681 cgccatcccg gcagctcatc gttctggatt gccctggtcc tgctgtcgtg ggtcgcagtg
    31741 gtgggcgttt tccgctggct gggtcgcagt gccccctcga ccgcgagtgt gaaggcgggt
    31801 gcagcatgaa tctgatccag ctcaagaacc atctgaaaca gcatcatgag tgctcgctgc
    31861 gcgatctggc cctgcgcttc gacagcagtg ccgaggtgct gcgccatcaa cttgcactgc
    31921 tcgagcgcaa aggactggtg aagaaactgc cgtcgggcac gctgtgcagc agcggttgcc
    31981 gcagttgcgc cacggagcag atcgaactct atcgctgggt ggccgcagaa gaggggagtg
    32041 ccgtgccact gcgcttcggc aacagcggcc gcaactgaag ccagacggta tcgagcaggc
    32101 gcggctgtgc tgcggcggtc ggatgcaaac catccgcctg catcatcccg ggttgcgcag
    32161 cgacaccttc gagcagaaac ggcacgagcg gaatcgagtt ttccttcgca atctcctgat
    32221 attgaataaa aaaaggttcg ctataacggc tgccgtagtt gggtggaatg cgtataccca
    32281 ccaacagcgg cctggcgcca cgctgctgga ccagttgcac catccgcgtc agattggcgc
    32341 gaatcgaatc gagcgcaaat ccgcgcaacc cgtcgttggc gcccagttcg atgatcacca
    32401 gtgacggtgt gtggcgatcg agcagatcag gcagtcgccg cagaccgcca tcggtggtct
    32461 caccgctgat ggaggcattg atcacctcga ccggaaatgc ttcggcggcc agtcgctgct
    32521 tcagcagctc gacccagccc tgatcgaggg tcaggccata accagcactc aggctgtcac
    32581 cgagcaccag cagggtt
//
LOCUS       DAORCQ010000021        31348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_773_length_31348_cov_6.264532, whole genome shotgun sequence.
ACCESSION   DAORCQ010000021 DAORCQ010000000
VERSION     DAORCQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 31348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31348
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_31348_cov_6.264532"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKI23_03640"
     CDS             <1..359
                     /locus_tag="PKI23_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNN36067.1"
                     /translation="EISPYLDYAFEVIGLPVTVQQAFAMIRPGGMAVVIGVGTGHIAE
                     IPLGDFLQEKRLVGSIYGSGQVHVDIPRLVDLYMEGKIMLDQLVSQVRPLNEVNQAIK
                     ALKAGEVARTLLSMAL"
     gene            821..2107
                     /gene="gltA"
                     /locus_tag="PKI23_03645"
     CDS             821..2107
                     /gene="gltA"
                     /locus_tag="PKI23_03645"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNN36068.1"
                     /translation="MTDKKATLTIPGMAPIELPIYSGTLGADVVDVRDLTNRGLFTYD
                     PGFVSTASCESKITYIDGEQGVLLHRGYPIEQLAEQSDYLETCYLLLKGELPNPQEKE
                     AFVSTIKNHTMVHEQIRSFYNGFRRDAHPMAIMCGVVGALSAFYHDSTDINNPLHREI
                     AANRLIAKMPTMAAMCYKYSVGQPFMYPQNSLSYAENFLHMTFGVPCEPVNINPVLAK
                     AMDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIAALWGPAHGGANEAVLIML
                     DQIGSSANIDKFVAKAKDKNDPFKLMGFGHRVYKNFDPRARVMRQTCYEVLDQLGIQD
                     PQLELAMKLEEIALKDPYFIEKKLYPNVDFYSGIILKAIGIPVTMFTVIFALARTVGW
                     IAHWNEMVGNSYKIGRPRQLYTGAQARDFVSIDQRG"
     gene            complement(2161..3135)
                     /gene="cysB"
                     /locus_tag="PKI23_03650"
     CDS             complement(2161..3135)
                     /gene="cysB"
                     /locus_tag="PKI23_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNN36069.1"
                     /translation="MKLQQLRYIWEVARHNLNVSQTAESLYTSQPGISKQIRALEDEL
                     GVEIFSRNGKHLTHVTPAGEAVLKVAGEILQRVESIRQLAQEFRDENKGSLSIATTHT
                     QARYALPPTIQRFIARYPDVSLHMHQGTPMQIAEMAAEGKVDFAIATEALDLFDALLM
                     MPCYRWNRSIVVPHGHPLTQLPRLTLEQVSRYPIVTYVFGFTGRSQLDDAFNAERLKP
                     RVVFTATDADVIKTYVRLGFGIGIVASMAYDPLQDRDLVALDASALFEPSITRIGFRR
                     GTFLRRYMYDFMQFFAPHLSRDLVDRAVACKSREEVEALFAQLVLPEH"
     gene            complement(3349..5001)
                     /locus_tag="PKI23_03655"
     CDS             complement(3349..5001)
                     /locus_tag="PKI23_03655"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNN36070.1"
                     /translation="MERESMQFDVVVVGGGPAGLSAACRLMQLAQEAGQELTVCLVEK
                     GSEIGAHILSGAVFEPSALNELFPNWQELDAPLKTPVKGDVFHYMLSPTSAAKVPNLF
                     IPKPMHNEGNYIVSLGNLCRWLATQAESLGVEIFPGFAASEILYNEDGSVKGIATGDM
                     GVGKDGSHKDSFTPGYELHAKYTLFTEGCRGHLGKQLIRKFNLDEGRDPGHYGIGIKE
                     IWEIDPAKHDQGLVVHTLGWPMDNQTEGGGFLYHAENNQLYLGYIVSLSYTNPHLSPF
                     EEFQRWKQHPKIRHYLEGGKRLTYGARAMNKGGLQSLLKMSFPGGLLAGCEAGTLNTA
                     KIKGSHTAMKSGMLAAEVLAEVLFAGDEGGKDLTQMQQKFETSWLYQELHATRNYGPA
                     LHKFGTFLGAAFVYVDQNLFGGKMPFTWRNPDPDHKAMKPAAECARIVYPKPDGVISF
                     DRLSSVYLSSTNHEEDQPCHLTLKDPAVPLRNNLPLYDEPAQRYCPAGVYEIVEETNG
                     DKRFQINAQNCVHCKTCDIKDPAQNINWVVPEGGGGPNYPNM"
     gene            5309..6061
                     /locus_tag="PKI23_03660"
     CDS             5309..6061
                     /locus_tag="PKI23_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNN36071.1"
                     /translation="MKVLVPVKRVVDANVKVRVLSDQTGIDLNNVKMAINPFCEIAVE
                     EAVRLKEKGVATEIVAVCMGPAASQEQLRTALALGADRAIHVETNAELQPLAIAKLLK
                     AVYDKEQPGLVIFGKQSIDADNGQTGQMFAALAGLAQGTQVSKIEVAGGSVTVTREVD
                     DGLQTIELKLPAVVTADLRLNEPRYASLPNIMKAKKKPLDRITPEDLGVDAAPRLTTV
                     KVEPPAERKAGIKVESVEQLVDKLKNEAKVIS"
     gene            6058..6987
                     /locus_tag="PKI23_03665"
     CDS             6058..6987
                     /locus_tag="PKI23_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNN36072.1"
                     /translation="MSVLVIAEHDNSTLKGATLNTVAAAKQIGGDVVVLVAGSGCAAV
                     ADQAAKVDGVSKVLLADNPAYAQQLAENMGLLIAELGKGYSHILAPASSNGKSALPRA
                     AALLDVAQISEITAVVSADTFVRPIYAGNAMATVKSGDPIKVITVRATGFDAAKAEGG
                     SAAVEAVGTAHNAGVSTFVSQEVVKSDRPELTAARVVISGGRGMQNGENFKLLEGIAD
                     KLNAAIGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYGLVADLFEAVPALDKLL"
     gene            7071..7814
                     /locus_tag="PKI23_03670"
     CDS             7071..7814
                     /locus_tag="PKI23_03670"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008007090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNN36073.1"
                     /translation="MNGKLAIIGGSGLTQLEGFQLSETRQIDTPYGTPSAPISCGHFH
                     GQALLFLPRHGAQHSLPPHLINYQANLLALQQAGADRVIAVNTVGAIPASLPPGILVF
                     PDQIIDYTWGRAHTYADLNRVEHVDFTHPYDAGLQQQLVEAASALSLPYSPAGVYGCT
                     QGPRLESKAEVDRLERDGCDLVGMTGMPEAALARELGLRYGCVCLVVNPAAGRGSGLI
                     TMEEINQVLDSGMAHVRQLLARLLQQMAA"
     gene            7937..9940
                     /locus_tag="PKI23_03675"
     CDS             7937..9940
                     /locus_tag="PKI23_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNN36074.1"
                     /translation="MQRLNPQQLAAVHYIDGPLLVLAGAGSGKTSVITRKIAYLVREC
                     GLSPRHIAAVTFTNKAAREMQARIKQLLQREGGGLMVSTFHTLGLTIVRQECSQLGLR
                     PGFSIFDEEESLTLIKEIMRRDDGAQDEQVALVRGLISSAKSGLQSPEQLRAMAATPL
                     EALVAEVYRHYQESLQAYNAVDFDDLIRLPVELLRNDATARERWQNRIHYLLVDEYQD
                     TNESQYQLIKLLVGSRAALSVVGDDDQSIYAWRGARPENLVELQRDFPTLKVIKLEQN
                     YRSTGTILKAANTLISRNPHLFEKALWSELGPGEPIRVLRLPNEEAEVERIVHGIIER
                     NLKQRVRYGDCAVLYRGNHQARLLELKLQSFRVPYRISGGTSFFARSEVRDLVAYLKL
                     IVNPDDDSAFLRIINVPRREIGPATLRALAEYAGARQISLLAACGELGLQSALPARAL
                     EKLRQFQQLLERSRRQIFTRNSLTAIRELVDEIDYPQWLRQNSKSEITAERRIANVEL
                     LLGSIGRQLGDDEEERDIESAVARLLLLDLLEQRDEEEDDNRVQLMTLHSAKGLEFAH
                     VYIMGMEEELLPHRNSIELGQIEEERRLAYVGITRARQSLTLTLTARRKRYQELFTTT
                     PSRFLDELPAELLDWEGRSDEDPKAKQQRGAAALSSLRHLLEQ"
     gene            complement(10005..10475)
                     /locus_tag="PKI23_03680"
     CDS             complement(10005..10475)
                     /locus_tag="PKI23_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNN36075.1"
                     /translation="MNHPVITIKRAMLATALIACAGWMGAVNAAEQSRTSHHSNTEAA
                     IAERIHPFGSVCVEGKECAANQGVAGAQVASAAKSGEEVFKASCFSCHGTGAAGAPKL
                     GDKAAWAPRIAKGMDTLLKSATSGLNAMPPKGLCMTCSDDELKGAIKYMVDGSK"
     gene            complement(10629..11513)
                     /locus_tag="PKI23_03685"
     CDS             complement(10629..11513)
                     /locus_tag="PKI23_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNN36076.1"
                     /translation="MTFFRRSPQSEASGGRLQGLTQTLGHYLAYGTLMRSALSQTFSR
                     FNGMIAQIQNSTHLLSDVMAQVRHNADEVQSHSQVMLLTAAETAQAQVGLVDSFSARL
                     EESHQAVHKGQRVHGEMEQFSQAFVAIGQMAQVITDIADHTNLLALNAAIEAARAGDA
                     GRGFAVVADEVRALARKSNHSSQQIQQAIEDLRQRMAACLHLLTDLVGTVTTLEEHMV
                     EVSGIATQTLEQANSVRHASETINQSVTRQCEAIDQAAEVSARMLTLTEQARQTTTSL
                     GEAMAAAWQVYQAGGHGR"
     gene            complement(11522..12316)
                     /locus_tag="PKI23_03690"
     CDS             complement(11522..12316)
                     /locus_tag="PKI23_03690"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN36077.1"
                     /translation="MRTSAQLRPADWSHSETIYQDQDHRFIWLGWEEEEDEGMVQTNQ
                     YLIIHKGEGILLDPGGVQVFPRVIGNVAQHIDIARIRHIFFTHQDPDVSGGIALWAGA
                     TPAQIHLSKLWGRFVPHLGQVDESRFTSVDDRGGVIALPGMELQLIPSHFMHSVGCLS
                     LWDPRARILFSGDVGTAVFPPNDRYLRVENFDRHRQRMEGFHRRYMVSQSVCRRWVGM
                     VRRLEPQIIAPQHGAIFPAPAIAPFLSWLENLRCGIDLLDDWYGAA"
     gene            12567..12643
                     /locus_tag="PKI23_03695"
     tRNA            12567..12643
                     /locus_tag="PKI23_03695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12601..12603,aa:Arg,seq:ccg)
     gene            complement(12792..13094)
                     /locus_tag="PKI23_03700"
     CDS             complement(12792..13094)
                     /locus_tag="PKI23_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNN36078.1"
                     /translation="MAVELLRDSDGSIQLIGQLDFDNVIECRRRGEQLLDDSASPVRI
                     SLARLERPSSIVVSLLLGWQRHALRQQKPLLFQSPPGALRQIITVCGLDELFAFDG"
     gene            complement(13099..13782)
                     /locus_tag="PKI23_03705"
     CDS             complement(13099..13782)
                     /locus_tag="PKI23_03705"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNN36079.1"
                     /translation="MTRPRPHSNFQRGINAMIRHLLLALLLLLPWSGPVLAAEPPPDQ
                     VIQQITDRMIALILQEKSRFDREPQHFYQAIDQLMTPASDFDRIAKGVMGKYYRQASD
                     AQRKRFAGVFKERMLQTYAKAFMNFDNQKIVVLPYRPQPGDEPGQASVMMEVHGHDGT
                     IYPVAYSMFLDDARQWRVQNVIVNGINLGLTFRSQFASSMDQKQNIDQVIDGWSAELD
                     EVNKVTRKG"
     gene            complement(13779..14249)
                     /gene="mlaD"
                     /locus_tag="PKI23_03710"
     CDS             complement(13779..14249)
                     /gene="mlaD"
                     /locus_tag="PKI23_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNN36080.1"
                     /translation="MQTRTLEILVGLFLLLGAAALTGLALRVSGLSLTAPEATYTLYA
                     DFQNIGGLKVRAPVTVAGVEIGSVTTITLDQQRYVGHVTMQINASVNALPVDSAAMIL
                     TQGMLGQQYIGISIGAEEKMLSDGNRFKSTQSALVLEDLIGKFLTSSIDKSGSK"
     gene            complement(14252..15028)
                     /gene="mlaE"
                     /locus_tag="PKI23_03715"
     CDS             complement(14252..15028)
                     /gene="mlaE"
                     /locus_tag="PKI23_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNN36081.1"
                     /translation="MALLQRLGRATLEFLVIWGRAGNLLLHSLAVWPSLQGLPLLVKQ
                     IHFVGVLSLVIILVSGLFIGMVLGLQGYTILSDYGTTSAVGQLVALSLTRELAPVVTA
                     LLFAGRAGSALTAEIGLMKATEQLASMEMIGVDPLRRIIAPRLLAGFISLPLLTMIFC
                     MVGVWGGKLVAVDWLGLHAGTFWSTMQANVSFRDDVVNGLLKSLAFAFVVVWIAVVQG
                     YECLPTSEGISRATTKTVVFSSLAVLGIDFILTAVMFGNL"
     gene            complement(15028..15843)
                     /locus_tag="PKI23_03720"
     CDS             complement(15028..15843)
                     /locus_tag="PKI23_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN36082.1"
                     /translation="MARTTDNFVEIRNLTFRRGSRAIFDNVDFDIRRGAITAVMGPSG
                     CGKTTLLRLIGAQLRPDAGAIRVDGEDIARLNRRRLFEVRRKMGMLFQSGALFTDLSL
                     YENVAFPLRAHTRLPEEMIRDLVLMKLHAVGLRGARDLMPAELSGGMARRAALARAIA
                     LDPAMIMYDEPFAGQDPISMGVLVELIKLLNHALGITSVIVSHDLVETMEIADYIYVI
                     ANGKIIGQGRPDELKNSDSALVRQFLTGSPDGPIAFHYPAPDYRTDLLGGQPW"
     gene            16050..17033
                     /locus_tag="PKI23_03725"
     CDS             16050..17033
                     /locus_tag="PKI23_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNN36083.1"
                     /translation="MKTDYRTFDFLGSARRTLTLESAALTALEARLDMAFVQACLLML
                     DCQGRVVVTGMGKSGHVANKISSTLASTGTPAFFVHPAEASHGDLGMITRQDVVLALS
                     NSGTTAEVLAIVPLIKRMQARLISMTGDAASPLARAAEVHLDVAVAQEACPLGLAPTA
                     STTTALAMGDALAVALLEARGFTAEQFAFSHPGGSLGRRLLLKVEDLMHGDESVPRVT
                     ASTPLRDALAEISRCGFGLTSVVDGQGRMIGIFTDGDLRRVLDRGLDVRATSVAEVMT
                     PKGAVVQPEQLAVDALQCMEARQIMALIVVDEERRPVGVLHMHDLVRSGVV"
     gene            17064..17588
                     /locus_tag="PKI23_03730"
     CDS             17064..17588
                     /locus_tag="PKI23_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNN36084.1"
                     /translation="MSDKLLQKIERIRLLALDVDGVLTDGRLYFLPDGGEIKVFNSLD
                     GHGLKMLQRSGVTVALITGRSSTAVARRAAELGISHLHQGSEQKLPIFDGLLEQLGLD
                     YSQAAFMGDDLPDLPLIRRAAVGMTVANAHPLVRQSADWQSTLAGGQGAVREACDLIM
                     QTQGTLCRLLQAYE"
     gene            17597..18178
                     /gene="lptC"
                     /locus_tag="PKI23_03735"
     CDS             17597..18178
                     /gene="lptC"
                     /locus_tag="PKI23_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNN36085.1"
                     /translation="MRANTWWIGCILLLCGGLLLKVSLDMDASRQRGGLVDDNQPDFF
                     MARAATRIYDEEGRVAYLLSAERWSHVRGEPGISLAQPNLIAHRTESSQRWQAQAERG
                     YWVESDGVLTLDQAVTFELWAQQRRLQQLLTPHMQIDYRRRTAHTDAPVRLLAEQGEM
                     SGNGIDIDLVAGKAVLPSQVRGHYLPSQPGASK"
     gene            18175..18708
                     /gene="lptA"
                     /locus_tag="PKI23_03740"
     CDS             18175..18708
                     /gene="lptA"
                     /locus_tag="PKI23_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNN36086.1"
                     /translation="MKKNLLSLRRLLLLSLLPWLPSTLLALSGDSRQPIEIVSDRAEF
                     DDNSASAHYIGNVVVTQGSLRIEADDLLLISDKGSVTEVKASGTPARFHQQLDGQEPL
                     MMEGEATSISYHAKSRQLELKGTAKLRQGERFFYGERIEYQLEKRLMRAFGGSEAKGE
                     GKRVRLILPPPKDEATP"
     gene            18705..19463
                     /gene="lptB"
                     /locus_tag="PKI23_03745"
     CDS             18705..19463
                     /gene="lptB"
                     /locus_tag="PKI23_03745"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNN36087.1"
                     /translation="MTVAASEQARADSCLNVSHLCKRYQKRQVVRDVSLTLRSGEIVG
                     LLGPNGAGKTTCFYMIIGLVRGDGGTITLDGQEITHLPMHRRARRGLGYLPQEPSVFR
                     QMSVADNILAVLETRARLSRSEQRQRLDQLLDEFNIAHLRNELGMSLSGGERRRVEIA
                     RALAVEPRFMLLDEPFAGVDPISMSDIKAIVRQLQQRSIGVLITDHNVRETLDICERA
                     YIVSDGGIIAEGDPAAIMQNRQVREVYLGKEFSL"
     gene            19506..20993
                     /locus_tag="PKI23_03750"
     CDS             19506..20993
                     /locus_tag="PKI23_03750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNN36088.1"
                     /translation="MKPSLQLKLGQSLTMTPQLQQAIRLLQLSTLDLHQEIQQALDSN
                     PMLELVEDEDERRADESIDAYGEQTSAEADDSGDLDGAEFDSHGDDEWSEAIPEDLSI
                     DTSWDEIYQSSGTGSSNPESDEDFESRNSVTESLQDHLRWQINLTHLSDVDRLIALNL
                     IDGINPDGMLTTSIEDVQLSLARDQIEVDLDEIEAVLHLVQQLDPIGVGARTLAECLL
                     LQLRQFPADTPWRKQAEKILTDHMAALGSRDYSQLMRLLKLTSEELGQVVQLIQQLNP
                     RPGSIISSSQSEYIIPDVYTRKVRGEWVVELNPESMPRLRVNEHYAAMVRRADNGADN
                     TFLRNNLQEARWFLKSLTSRNETLLKVARKIVEVQRGFLDHGAEAMKPLVLYDIAEAV
                     GMHESTISRVTTQKFLHTPRGIFELKYFFSSHLETDSGGECSSTAIRAVIKKLISAES
                     AKKPLSDSKITAILSQQGIKVARRTIAKYRESMGIAPSNERKQLP"
     gene            21048..21356
                     /gene="raiA"
                     /locus_tag="PKI23_03755"
     CDS             21048..21356
                     /gene="raiA"
                     /locus_tag="PKI23_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNN36089.1"
                     /translation="MQINVSGHHVEVSPALHDYVTSKMNRIERHFEQITSIDVILSVE
                     KQRQKAEATIRFSGGEIFADIESDDMYAAIDAMADKLDRQMIKHKEKSIGRHHGGSNR
                     "
     gene            21434..22354
                     /gene="rapZ"
                     /locus_tag="PKI23_03760"
     CDS             21434..22354
                     /gene="rapZ"
                     /locus_tag="PKI23_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNN36090.1"
                     /translation="MDTRTRLVIISGRSGSGKSTALHVLEDLGFYAIDNLPASLLNTL
                     AAGVAAGQYAEQRRIAVSIDARNVPGDLSRLPEILEEVSSITLTPQILFLDADDQTLL
                     QRFSETRRRHPLTGPHLPLTEAIGRERELLRPVAALADVTLDTSRLSLHDLRDLIRRR
                     FSTTEGENFSLLFESFGFKKGTPADADFVFDVRALPNPYWHTALRVHDGREPPVIEFL
                     EAQPEVRQMLDDIGTFLDRWLPRFLASGRSYVTVALGCTGGQHRSVYLCERLAERFCG
                     RFPTVLLRHRELSNAREPVQVAVASAPETG"
     gene            22468..23832
                     /gene="mgtE"
                     /locus_tag="PKI23_03765"
     CDS             22468..23832
                     /gene="mgtE"
                     /locus_tag="PKI23_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNN36091.1"
                     /translation="MADSNQGARARTQTQLQALSSALESGTFVQIRRMLNGVPAVDVA
                     RLLESSPPNLRDLLWELVDKEKEGEILQHLSDELQGLFLRRMNAETVASVVEGFDTDD
                     LADLLQQLPERVIREVLDAMDAQDRHRVERVLAYPEDSAGGLMNTDTITVRPRFTLDV
                     VFRYLRRHDSLPPMTDNIIVVNSEDRFIGLLPLTKMLVSDPSITVREIMVTDVAAIPA
                     STSASEVAKLFERHDWVSAPVVDETGHLLGRITIDDVVDVIIEAADQQVMSMAGLGDE
                     ESTFIAPFKAATQRATWLGANLLTGFIAAGVVGLFAETIHQVVALAVLMPIIPSMGGV
                     AGSQTLTLMIRGMALGHIGRANRFWLLRQELLIGLLNGLLWATIVGVATAYWFDDRLL
                     GVILAVAMLCNLLAAALTGVLLPLMLRTFGIDPILAGSVLLTTVTDVVGFVVLLGLAS
                     WLLL"
     gene            23848..24360
                     /gene="yjgA"
                     /locus_tag="PKI23_03770"
     CDS             23848..24360
                     /gene="yjgA"
                     /locus_tag="PKI23_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNN36092.1"
                     /translation="MQAPEEKPSKSARKRRMHELQRLGGTLLQFSDAQLRRLPLTEAL
                     REAVREGKRLKAPAALQRHCQFLGRLLDEADNEALLIAIVTLEQGTQRADPWHHQAER
                     LRDALLAGETVHFQAWLVQLDPADQAQVNAQAERARQAVRRGQEVARESRQLYRLLHR
                     LLEAAASASD"
     gene            complement(24361..24630)
                     /locus_tag="PKI23_03775"
     CDS             complement(24361..24630)
                     /locus_tag="PKI23_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36093.1"
                     /translation="MSTLQEDLKQLAAQLKVERDALHLQAHLLKLDLLDEWQKSEKKW
                     EQLNQKLQRAGAEGVASGQEIGAAVKLLGEELKSAYQRINRQLKG"
     gene            complement(24781..>27198)
                     /locus_tag="PKI23_03780"
     CDS             complement(24781..>27198)
                     /locus_tag="PKI23_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HNN36094.1"
                     /translation="SDRIQLGSDRFGAQGVFRLLLPRHDLDAATLGLSIGINPASLLE
                     KDQFLPTLTENARLIDWIRQSLTTGLLEQGSVVYQGRLHGPEQGKRTLLFFRGRDMRL
                     RFQPDWPILEELAGSVWVSDANLDIEVIKGKSGNLALTQGKVVGRRSDRNPHYHLSIE
                     SDLTSTLAESKKWVLTTPLRQELLPWLGELELHGPLQGRLKLDVPLGEKPKIGYQLDL
                     RTNNGRVAAPRYRLRYDQVEGALRLATDQAVHADQIKARFLGQPVTVRLQQSSDPTGH
                     TTLLLTQEGRVDVADLTDWLDLQPLDFFQGEAPYVAQLQLPLTGGNGSELTFESNLVG
                     LGSSLPPPLSKAPQQAVPLFLLTRFNSAGLESLSLLLNEEIGFDLTFDAAGRDRGQIL
                     LGSSQSARPLSAHNPLRGNLPPIDLDQWIAFYRAHLAHIDGIEKSVDWFTDVGKIDFT
                     LPQLRFLGQALNEVHLTISQQQERVDLRVASDKVRGTLQLALDHSAPIRIALEQLTLT
                     TASNRPDNDPLANFDPATLPSLDFQAAAIHIDEQPLGSWAFQLRPTAQGITAQQINAT
                     IDGFTLRNALMVWQRDTGGLHRSQLTSTLEGGDIAQLARYFGVVPGISSQSARIDVNL
                     AWPGSPAHFALERTRGDYRLLFRNGRFETGGGTSPLGLFSLFNFNNLISKLQLNLSDF
                     GGGSIGYQQVDGRMKLANNVLTLDDRINIESAVGRFKLAGTIDLASQNLDLDMAMTLP
                     IGTNLPWYVAAIGGLPTAAAAYLAGQLFEKPLAQLTSTRYQVTGPINDPKITLVELFQ
                     DQPTRNR"
     assembly_gap    27199..27298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27299..28741)
                     /locus_tag="PKI23_03785"
     CDS             complement(<27299..28741)
                     /locus_tag="PKI23_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36095.1"
                     /translation="MTPWRLITLALQALLWLGFALLLGAAVVVGVGRNLLPRIDEFQS
                     QLSAEANRRCQCRITFTQITGDWEGLAPVIRLQGLEIETANWSLRGDAVEFSLHLLQS
                     LWHRQPRLVALSARGVTLSVDAGAGGRARPLDALLVPLGLPERLQLHDVTLLWQDAAA
                     TSTVPPLLLPQLDLEQAAAGYRIAADFKLDATAPVTTAILHLHGFDGSSLPSTGEFHL
                     RLPQQPIDARWLRLLGGFEPPAHLTATAELWGDLEGLQLVGLTGEISAAVAPTATAAS
                     ADAPVIQLGFHYQHESSATPTASLARPAWRLELEPRLLLPNEAPRTTHLHLIGAGAPD
                     QAAIELFADRIDLRPWRSWLIGSNLLPSAWQQRLEQLAPTGQIEALHLLAPRLTTDAF
                     DDLELSARLTTFSMQAWQGAPAVSGVDGTLFIRGGHGLLQFDSKQLDLHFPKLFAQSW
                     RIDTAQGTLFWQVTPELIEIDSDRIQLGSDR"
     gene            complement(28805..29422)
                     /locus_tag="PKI23_03790"
     CDS             complement(28805..29422)
                     /locus_tag="PKI23_03790"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008092504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNN36096.1"
                     /translation="MDAPGFVYLASASPRRRALLEQIAVPCRLLAVNVDESPLAGESS
                     PAHVLRLAQAKAETGRDSLPLADAPVLGADTVVVAEADEILGKPRDRDHFMAMMTQLS
                     GRWHRVISAVAVVTATACHTAVVTTRVRFRPLDPAELARYWESGEPLDKAGGYAIQGL
                     AAPFVERIEGSYSAVVGLPLRETQALLARCGMVTWPALDRSGSRC"
     gene            complement(29423..29911)
                     /gene="mreD"
                     /locus_tag="PKI23_03795"
     CDS             complement(29423..29911)
                     /gene="mreD"
                     /locus_tag="PKI23_03795"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNN36097.1"
                     /translation="MRSSPIGNWLVILLTFVIALLLEVVPLPADWQPWRPTWALLCLI
                     YWTMALPERIGLLFGWLLGLPLDVLHDVTLGQHGLALMVVSYIDLSLHQQLRLFPRVQ
                     QALIVCVLVSLYLLLLSLMQLLISQQPWRSDRLLSALSSGLLWPLLFPLLRRLRRHYR
                     VE"
     gene            complement(29901..30758)
                     /gene="mreC"
                     /locus_tag="PKI23_03800"
     CDS             complement(29901..30758)
                     /gene="mreC"
                     /locus_tag="PKI23_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNN36098.1"
                     /translation="MFVKRPRLGLRLLLLGLLSVGLMVADAHYHALDLLRERLDWALA
                     PMIELADWPLRLYAGSRDSLSSRQQLARENDALRTERLLLSQKAQKMAALQAENIRLR
                     QLLNSTTHLDENALIAEIIGVSSDRSRHFVIIDRGAKSGIRKGQPLLDASGIMGQVIS
                     VRPHHSRVLLITDQNHAVPVQVLRNGVRAIAVGTGDDSHLELLNVPETSDIEVGDQLV
                     TSGLGGRFPANYPVGVVESVNRNAGQPFTQIRAIPTAQLAISRHVLVILTTPQPGQNS
                     VTEATDDAQ"
     gene            complement(30824..>31348)
                     /locus_tag="PKI23_03805"
     CDS             complement(30824..>31348)
                     /locus_tag="PKI23_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNN36099.1"
                     /translation="AIISLSGIVYSDSVRIGGDRFDEAIITHVRRQYGSLIGDATAER
                     IKQEIGIAFASVESSEIDVRGRNLAEGIPRSFTLDSEEILEALQEPLSGIVQAVKRAL
                     EQSPPELASDIAESGIVLTGGGALLRGLDQLISKETGLPVRVADDPLTCVARGGGRAL
                     EMMDASNFELLSSE"
BASE COUNT         5798 a         9654 c        10005 g         5791 t
ORIGIN      
        1 aggagatcag cccctacctc gactacgcct tcgaggtgat cggtctgccg gtcactgtgc
       61 agcaggcctt cgcgatgatc cgcccgggcg gcatggctgt cgtcatcggg gtgggcaccg
      121 gtcacattgc cgagattccg ctcggcgact tcctgcagga gaagcggctg gtcggttcga
      181 tctacggctc cgggcaggtg catgtcgaca ttccccggct ggtcgacctc tacatggagg
      241 gcaagatcat gctcgatcaa ctggtctcgc aggtgcggcc tttgaacgag gtgaatcagg
      301 cgatcaaggc actgaaagcg ggcgaagtgg cgcgcacgct gttgtcgatg gcactgtgat
      361 cgctcaccgg ccagtctgag cggccattca cgctgttcag gctggccttc aatcctccgc
      421 aagcgctggg ggtgcggtca gccaccgcaa tcgtgcccgg tcggtccctt ttggatgcct
      481 catgcgcatc tgcgatgccc tttcaggcgc cacaaccggg cagattcgtc gcgatcggca
      541 ctgctctgtg cagaaaatca ctccgaagca gcccgtgcag cagggggcag acacgaacag
      601 ggagcggatt ttgcagggta aaaggcattc cacttggcat gtcggccgat tttcagcaac
      661 cccggcctcg actagaatgg tgcacacaaa cattcaatga tgcaggttca tgaccccatt
      721 ccatctgtcg cgctctcccc tgaaaaccac tgcccttcgt gtcatgtgtc gcccacgccg
      781 ctgaccagtt ccattcgtat tcaccgcagg agccccgtta atgaccgata aaaaagccac
      841 cctgaccata ccgggaatgg cgcccatcga actgcccatc tattccggta cgctgggtgc
      901 tgacgtcgtc gacgtgcgtg acctgaccaa tcggggactt ttcacctacg atccgggttt
      961 tgtctcgacc gcctcctgcg aatcgaagat cacctacatc gatggcgagc agggcgttct
     1021 gctgcaccgc ggctacccga tcgagcagtt ggcggagcag tctgactatc tggagacctg
     1081 ctacctgctg ctgaagggcg aactgcccaa cccgcaagaa aaagaggcct ttgtcagcac
     1141 catcaagaat cacacgatgg tgcatgaaca gatccgctcc ttttacaacg gttttcgccg
     1201 cgatgcccac ccgatggcga tcatgtgtgg cgtcgtcggc gcgctgtcgg ccttctatca
     1261 cgactccacc gacatcaaca atccgctgca tcgggagatc gcggccaacc ggctgatcgc
     1321 caagatgccg acgatggccg cgatgtgcta caagtattcg gtcggccagc cgttcatgta
     1381 tccgcagaac agcctctcgt atgccgagaa tttcctgcac atgacctttg gtgtgccgtg
     1441 cgagccggtc aacatcaatc cggtgctggc caaggcgatg gaccgcatct tcattctcca
     1501 tgctgaccac gagcagaacg cctcgacctc gacagtgcgg ctggcgggct catcgggcgc
     1561 caatcccttt gcctgcatcg ccgccggcat cgccgcactg tgggggccgg cccatggcgg
     1621 cgccaacgag gcggtgctga tcatgctcga tcagatcggt tcgtcggcca acatcgacaa
     1681 gttcgtggcc aaggccaagg acaagaacga tccattcaaa ctgatgggct ttggccatcg
     1741 ggtctacaag aacttcgatc cgcgcgcccg ggtgatgcgc cagacctgct acgaggtgct
     1801 cgatcagctc ggcatccagg atccccagct cgaactggcg atgaagctcg aggagatcgc
     1861 gctgaaggat ccttacttca tcgagaagaa gctctacccc aacgtcgact tctattccgg
     1921 catcattctg aaggccatcg gcattccggt gacgatgttc acggtgatct tcgcgctggc
     1981 ccgcaccgtc ggctggattg cccactggaa cgagatggtc ggcaacagct acaagatcgg
     2041 tcggccgcgg cagctctaca ccggcgcgca ggcacgcgac ttcgtgtcga tcgaccagcg
     2101 cggctgagtg gccgccctct cgcagcgcca ttcgccatcc atgcaccggc gatggcgctg
     2161 ctaatgttcg ggcagcacca gttgcgcgaa cagcgcctcg acctcttccc ggcttttgca
     2221 ggccaccgcc cgatcgacca gatcgcggct gaggtgcggg gcaaaaaact gcatgaagtc
     2281 atacatgtag cgccgcagaa aggtgccgcg ccgaaagccg attcgggtga tgctgggctc
     2341 gaacagcgcg ctggcatcca gtgcgaccag gtcgcggtcc tgcaacgggt cgtaagccat
     2401 cgaggccacg atgccgatgc cgaaacccag gcgcacgtag gtcttgatca catcggcgtc
     2461 ggtggcggtg aacaccaccc tgggcttcag ccgttcggcg ttgaaggcgt catccagttg
     2521 tgagcggccg gtaaagccga agacataggt gacgatcgga tagcgggaaa cctgttccag
     2581 cgtgagcctg ggcagttgcg tcaatggatg cccatgcgga acgacgatgg agcggttcca
     2641 gcgatagcag ggcatcatca gcagcgcatc gaacagatcg agcgcctcgg tggcgatggc
     2701 aaaatcgacc ttgccttccg ccgccatttc ggcgatctgc atcggcgtgc cctgatgcat
     2761 gtgcagcgag acatcgggat agcgtgcaat gaaccgctga atcgtcggcg gcagggcgta
     2821 gcgtgcctgg gtgtgggtgg tggcgattga caggctgccc ttgttctcgt cgcgaaactc
     2881 ctgggccagc tgccggatcg actcgacgcg ctgcagaatc tcgcccgcga ctttcagcac
     2941 cgcctcaccg gccggcgtga catgggtcag gtgcttgccg ttgcgtgaga agatctccac
     3001 ccccagttca tcctcgagag cgcgaatctg cttgctgatg cccggttgtg aggtgtagag
     3061 gctctcggca gtctgcgaga cgttgaggtt gtgacgggcc acttcccaga tgtagcgcag
     3121 ttgttgcagc ttcatgtctg ctccggttcg ctgtcggttg ccatgtccgt accgtgtctg
     3181 ggtcggtcag ttcagttata actcaaaaca cgatttattc catctttgca gcgtcagaca
     3241 gccagtcatc cagccgaggc cgtcaggcat gactgtaaac gacaagggcc tgcattgcgc
     3301 aggcccttgt cggtctgaat cggttcggaa ccgaacggcg ttttgccgtt acatgttggg
     3361 gtagttcgga ccgccgcccc cttcaggcac cacccagttg atgttctggg ccggatcctt
     3421 gatgtcgcag gtcttgcagt gcacgcagtt ctgcgcgttg atctggaagc gcttgtcgcc
     3481 gttcgtctct tcgacgatct catagacgcc ggccgggcag tagcgttgcg ctggctcgtc
     3541 gtagagcggc agattgttgc gcagcggcac cgcagggtcc ttcagcgtca ggtggcaggg
     3601 ttgatcctct tcatggttgg tgctggagag gtagaccgat gacagccgat cgaagctgat
     3661 caccccatcg ggtttcgggt agacgatcct ggcgcactcg gccgccggtt tcattgcctt
     3721 gtggtcgggg tcggggttgc gccaggtgaa gggcatcttg ccgccgaaga ggttctggtc
     3781 gacatagaca aaagcagcac cgaggaaggt gccgaacttg tgcagggccg ggccgtagtt
     3841 gcgggtggca tgcagctcct gatagagcca tgaagtctcg aacttctgct gcatctgcgt
     3901 cagatccttg ccgccctcgt cgccggcgaa gagcacctcg gccagcacct cggctgccag
     3961 catgcccgac ttcatcgcgg tgtgcgaacc cttgatcttg gcggtgttga gcgtgcccgc
     4021 ctcacaaccg gccagcaagc cgccggggaa cgacatcttg agcagcgact gcaggccacc
     4081 cttgttcatt gcccgcgcgc cataggtcag gcgcttgccg ccttcgaggt agtgtctgat
     4141 cttcggatgc tgcttccagc gctggaactc ctcgaacggg ctcaagtggg gattggtgta
     4201 gctcagcgag acgatgtagc cgaggtagag ctggttgttc tcggcatgat agagaaagcc
     4261 accgccttca gtctggttgt ccatcggcca gcccagcgtg tgcaccacca gcccctgatc
     4321 gtgcttggcc gggtcgatct cccagatctc tttgatgccg atgccgtagt ggcccggatc
     4381 gcgcccttcg tcgaggttga attttctgat cagctgcttg ccgaggtggc cacggcaccc
     4441 ttcggtgaag agcgtgtact tggcatgcag ctcgtagcca ggcgtgaagc tgtccttgtg
     4501 gctgccatcc ttgcccacac ccatgtcgcc ggtggcgatg cctttgacgc tgccatcttc
     4561 gttgtacagg atctcggaag cggcaaagcc ggggaagatc tcgacgccga gcgactcggc
     4621 ctgggtggcc agccagcggc agaggttgcc gaggctgacg atgtagttgc cctcgttgtg
     4681 catcggcttg gggatgaaca ggttcggcac cttcgccgcg ctggtcgggc tcagcatgta
     4741 gtggaacacg tcacccttga ctggcgtctt cagcggtgcg tcgagctcct gccagttggg
     4801 aaacagctca ttgagtgccg aaggctcgaa aaccgcaccg gagagaatgt gcgcgccgat
     4861 ctcggaaccc ttctcgacca ggcagacggt cagctcctgt ccggcttcct gcgccaactg
     4921 catcaaacgg caggcggcag agaggccggc aggccccccg ccaaccacca ccacatcaaa
     4981 ttgcattgat tcacgttcca cggcgatcac tcctcactct tgagctgggc ttcttcctgg
     5041 ctgctttgtt tctgctgaac ctggctggcc ggtccggctg gccaacccag tcatccacga
     5101 agaacacgaa gaaaatgaca gcgggcgatt atagagcgac ccggaacaaa gaccaattca
     5161 aaagatgtgc agagcccctg cacggatgcc ctcgaatatt gacctcgggc gactaaacga
     5221 ggagaatcat gccgctttgc gcgtccgtca tgcccggtca tctgccgggt ttttatttat
     5281 tcacagctca attgggaaac ggcacatcat gaaggttctt gtcccggtaa agcgcgtcgt
     5341 tgatgccaat gtcaaggttc gggtgctctc ggaccagacc ggcatcgacc tgaacaacgt
     5401 caaaatggcg atcaacccct tctgcgaaat cgccgtcgaa gaagcggttc gcctgaaaga
     5461 gaaaggggtc gccaccgaaa tcgtcgcggt ctgcatgggc cccgccgcca gccaggagca
     5521 gttgcgcacc gcgctggccc tgggcgccga ccgcgccatc catgtcgaga ccaatgccga
     5581 actgcaacca ctggccatcg ccaagctgct gaaggcggtg tacgacaagg agcagccggg
     5641 cctcgtcatc ttcggtaaac agtcgatcga cgcggacaac ggccagaccg gccagatgtt
     5701 cgccgcactg gccggtctgg cccagggcac gcaagtctcc aagatcgagg tggccggtgg
     5761 cagcgtcacg gtgacccgtg aggttgatga tggcttgcag accatcgagc tcaaactgcc
     5821 ggcagtggtg accgccgacc tgcgactcaa cgagccgcgt tacgcctcgc tgccgaacat
     5881 catgaaggcg aagaaaaagc cgctcgatcg gatcacgccc gaagatctgg gtgtcgatgc
     5941 cgcaccgcgt ctgaccactg tgaaggtcga gccgcccgca gagcgcaagg ccggcatcaa
     6001 ggtcgaaagc gtcgagcaac tggttgataa actcaaaaac gaagcgaagg tgatttcatg
     6061 agtgttctag tcatcgccga gcacgacaac agcaccctca agggtgccac gctcaatacc
     6121 gtggccgctg ccaagcagat tggtggcgat gtggtggttc tggttgccgg cagcggctgt
     6181 gccgcggtgg ccgatcaggc cgccaaggtc gatggcgtca gcaaggtgct gctggccgac
     6241 aaccctgcct atgcccagca actggccgag aacatgggcc tgctgatcgc cgaactcggc
     6301 aagggctaca gccacattct ggcaccggcc tccagcaacg gcaaaagcgc cctgccgcgt
     6361 gccgccgcac tgctcgacgt ggcccagatc tccgagatca ctgccgtggt cagcgccgac
     6421 accttcgtgc ggccgatcta tgccggcaat gcgatggcca ctgtcaagtc gggtgatccg
     6481 atcaaggtga tcaccgtgcg tgccaccggc ttcgatgccg ccaaggccga aggcggttcc
     6541 gccgcggtcg aggcggttgg cactgcccac aatgccggcg tttccacctt cgtttcgcaa
     6601 gaggtggtca agtccgaccg tcctgagctg accgcagccc gcgtcgtcat ctccggtggc
     6661 cgtggcatgc agaatggtga gaacttcaaa ctgctcgaag gcatcgccga caaactcaat
     6721 gccgccatcg gtgcctcgcg tgccgctgtc gatgccggct tcgtgcccaa cgacatgcag
     6781 gtgggacaga ctggcaagat cgtggcaccc gagctttaca tcgccgtcgg catctccggc
     6841 gccatccagc acttggccgg gatgaaggac agcaaggtga tcgtcgccat caacaaggat
     6901 gaagaggcgc cgattttcca ggtggccgac tacggtctgg tggccgacct gttcgaagcg
     6961 gtccccgccc tcgacaaact gctttgagcg agcggcggcg tgacgatgaa aaacccggca
     7021 ctggccgggt ttttcatttc cagcaacgat tcaatcagtg aaaaatgcgc atgaacggca
     7081 aactggccat catcggcggc tccggtctga cccagctcga gggttttcag ctgagcgaga
     7141 cccgccagat cgacacccct tacggcaccc cctcggcacc gatcagctgt ggccactttc
     7201 acggtcaggc gctgctgttc ctgccgcgtc atggcgcgca gcattcgctg ccgccccatc
     7261 tgatcaacta ccaggccaac ctgctggcac tgcaacaggc cggtgccgac cgcgtcatcg
     7321 cggtcaacac ggtcggtgcc atcccggcca gcctgccgcc cggcatcctg gtgtttcccg
     7381 atcagatcat cgactacacc tgggggcgtg cccacaccta tgccgacctg aaccgggtcg
     7441 agcatgtcga cttcacccat ccctacgatg ccgggttgca acagcagttg gtcgaagcgg
     7501 ccagcgccct gtcgctgccc tacagtccag ccggtgtcta tggctgcacc caggggccac
     7561 ggctcgaaag caaggccgag gtcgaccgtc tggagcgcga cggttgcgac ctggtcggca
     7621 tgaccggcat gcccgaggcg gcactggcgc gtgaactggg gctgcgctac ggttgcgtct
     7681 gcctggtggt caacccggcg gctggtcgtg gcagcgggct gatcacgatg gaggagatca
     7741 accaggtgct ggacagcggc atggcgcatg tccgccaact gctggcgcgg ctgctgcaac
     7801 agatggccgc ttagctggct ggttcagtct ccacagtgag caacggcgcc tgagcagcca
     7861 gaaccgacgg cgcggctgtt gtcgccggca cgattgcgcc tgttaaaatc cgcccccttt
     7921 tctgaccggc gcagcgatgc agcgactcaa tcctcaacaa ctggccgcgg tgcactacat
     7981 cgacggccca ctgctggtgc tggcgggtgc cggcagcggc aagaccagcg tcatcacccg
     8041 caagatcgct tatctggtgc gtgagtgtgg cctgtcgccg cggcacatcg ccgccgtcac
     8101 cttcaccaac aaggccgcgc gtgaaatgca ggcgcgcatc aagcagctgt tgcagagaga
     8161 gggcgggggg ctgatggtct ccacctttca tacccttggc ctgacgatcg tgcgtcagga
     8221 gtgcagtcag ctcggtctgc ggcccggctt ctcgattttc gacgaggagg agagcctgac
     8281 gctgatcaag gagatcatgc gacgcgacga cggggcgcag gatgagcagg tcgcgctggt
     8341 gcgcggactc atctccagtg ccaagtcggg attgcagagt ccggagcaac tgcgggccat
     8401 ggccgcgaca ccgctcgaag cgctggtggc ggaggtgtac cggcattatc aggagagttt
     8461 gcaggcttac aatgcagtcg atttcgatga cctgatccgc ctgccggtcg agctgctgcg
     8521 caacgatgcc acggcgcggg agcgctggca gaaccgcatt cactacctgc tggtcgatga
     8581 gtatcaggac accaacgaga gccagtatca gctgatcaaa ttgctggtgg gcagccgtgc
     8641 ggcgctgagc gtggtcggtg acgatgacca gtcgatctac gcctggcgcg gtgcccggcc
     8701 agagaacctg gtcgagttgc agcgcgattt tccgacactc aaggtgatca agctggagca
     8761 gaactaccgc tcgaccggca ccattctcaa ggcggccaac acattgatca gccgcaatcc
     8821 ccatctgttc gagaaggccc tctggagcga actggggccg ggcgagccga tccgggtgct
     8881 gcggttgccg aatgaagagg ccgaggtcga acgcatcgtg cacggcatca tcgagcgcaa
     8941 cctgaagcag cgggtccgtt atggcgattg cgccgtgctc tatcgtggca accaccaggc
     9001 acggctgctc gaactcaaat tgcagagctt tcgtgttccc taccgcatca gcggcggcac
     9061 ctcgttcttt gcccgcagcg aggtgcgtga cctggtcgcc tacctcaagc tgatcgtcaa
     9121 tccggatgat gacagtgcct ttttgcgcat catcaatgtg ccgcgccgcg agatcgggcc
     9181 ggccacgctg cgtgcgctgg ccgagtatgc cggtgcgcgc cagatcagcc tgctggccgc
     9241 ctgtggcgaa ctgggattgc agagtgcgct gccggcacgg gccctggaga agctgcgtca
     9301 gttccagcag ttgctggagc gcagccgtcg gcagatcttc acccgcaaca gcctgaccgc
     9361 gattcgcgaa ctggtcgatg aaatcgacta cccgcagtgg ctgcggcaga acagcaagag
     9421 cgagatcact gccgaacggc gcatcgccaa tgtcgagctg ctgctcggtt cgatcggtcg
     9481 ccaactgggc gatgacgaag aggaacggga catcgaatca gccgttgccc gattgctgct
     9541 gctcgacctg ctcgaacagc gtgacgaaga ggaggacgac aaccgggtgc agttgatgac
     9601 gctgcactcg gccaaggggc tggagtttgc gcatgtctac atcatgggca tggaggagga
     9661 gctgctgccg caccgcaaca gcatcgagct gggacagatc gaagaggagc ggcggctcgc
     9721 ctatgtcggc atcacccgtg cccgccagag cctgacgctg acgctgaccg cccgccgcaa
     9781 gcgctaccag gagctcttta ccaccactcc gagccgcttt ctcgacgaac tgccagcgga
     9841 gctgctcgac tgggaggggc gcagcgacga agatcccaag gccaaacagc aacggggtgc
     9901 cgccgcactg tcgagcctgc ggcatctgct ggagcagtga cccctgaatg caacaggggg
     9961 ccttcgggcc ccctgttcag caaaatctgc gcgcctggag cggattattt gctgccatcc
    10021 accatgtact tgatcgcgcc cttcagttcg tcgtcgctgc aggtcatgca cagccccttg
    10081 ggcggcatgg cgttgaggcc actggttgcg ctcttcagca gggtgtccat ccccttggcg
    10141 atgcggggtg cccaggcggc cttgtcgccc agtttcggtg cgccggcggc gccagtgcca
    10201 tggcaactga agcagctggc cttgaacacc tcttcaccac tcttggcggc actggcgacc
    10261 tgcgcgccag cgacaccctg gttggctgcg cactccttgc cttcgacgca gaccgaacca
    10321 aacgggtgga tgcgctcggc aatcgcggct tcggtgttgc tgtggtggct ggtgcggctc
    10381 tgctccgcgg cgttgaccgc acccatccaa ccggcgcagg cgatcagcgc agtggccagc
    10441 attgcgcgct tgatggtgat aacaggatga ttcatcgggc acattctcca gtttgggtca
    10501 ttgaaaaaac ggatggcgat cgatcgaagc ctgtgccaag tatagtccga ttgccgtgtg
    10561 gaatcagcac cgtgcgcatg gagctgggca gtgggctctc ccggtttcgc catccatcct
    10621 gctcaaggtc agcggccatg gccgcctgcc tggtaaacct gccaggcggc agccatcgcc
    10681 tcgccgaggg aagtggtggt ctgtctggcc tgttcggtga gcgtcaacat ccgtgccgag
    10741 acctcggcgg cctgatcgat ggcctcgcac tgccgggtga ccgactggtt gatggtctcg
    10801 gaggcgtggc ggaccgagtt ggcctgctcc agcgtctgtg tggcgatgcc gctgacctcg
    10861 accatgtgct cttccagcgt ggtcaccgtc ccgaccaagt cggtgagcag atgcagacag
    10921 gctgccatgc gctgccgcag atcctcgatg gcctgctgta tctgctggga ggagtggttc
    10981 gacttccgcg ccagagcgcg cacctcgtcg gccaccaccg cgaagccgcg gcccgcgtca
    11041 ccggcgcggg cggcttcgat ggcggcattg agcgccaaca ggttggtgtg atcggcgatg
    11101 tcggtgatca cctgggccat ctggccgatg gcgacgaagg cctgggagaa ctgctccatc
    11161 tcgccgtgga cccgctgccc tttgtgtacc gcctgatgcg actcctccag ccgcgcggag
    11221 aaagagtcga ccagccccac ctgcgcctgt gcggtttcag ccgccgtcag cagcatcacc
    11281 tggctgtggc tctgcacttc gtcggcattg tgtcgtacct gcgccatcac gtcggagaga
    11341 agatgggtgc tgttctggat ctgggcgatc atgccgttga aacgactgaa ggtctgactc
    11401 agtgcactgc gcatcagggt gccataggcc agatagtggc ccagggtctg ggtcagaccc
    11461 tgtaagcggc ctccgcttgc ttctgattgc ggcgagcgcc gaaaaaatgt caacctggcc
    11521 atcatgcagc cccataccag tcgtcgagca ggtcgatgcc gcagcgcagg ttctcaagcc
    11581 atgacagaaa gggcgcaatg gcgggtgccg ggaagatggc gccatgctgg ggggcgatga
    11641 tctgcggctc aaggcgtcgt accatgccga cccagcggcg gcagacactc tgactgacca
    11701 tgtagcggcg atgaaaacct tccatgcgct ggcggtggcg atcgaagttc tccacccgca
    11761 ggtagcggtc gttcggcggg aagaccgcgg tgccgacatc gccggagaag agaatcctcg
    11821 cgcggggatc ccacaggctg agacaaccga ccgagtgcat gaagtgcgag gggatcaact
    11881 gcaactccat gcccggcagt gcgatgacgc caccccggtc atcgaccgag gtgaagcggc
    11941 tctcgtcgac ttggcccagg tgcgggacga accttcccca cagcttggag agatggatct
    12001 gggctggagt ggcgccggcc cacagggcga tgccaccgga gacatcggga tcctgatggg
    12061 tgaaaaagat gtggcgaatc ctggcgatat cgatgtgttg cgccacattg ccgatcaccc
    12121 gcggaaagac ctgcaccccg cccggatcga gcaggatccc ctcacccttg tggatgatca
    12181 gatactggtt ggtctgcacc atcccttcat cctcttcctc ctcccagccg agccagatga
    12241 agcggtgatc ctgatcctga tagatggttt cggagtggct ccagtctgcc ggtcgcaact
    12301 gcgcgcttgt tcgcatgatt tgcctcgttt tgaaagaatc agcgggtggg gctgatcatg
    12361 agaagcatag gagaatatct ggagtcgctt cgggcgcgca ttttcgccag atgcccgccc
    12421 gggccgcgtt ggagtctgtc ctgcgacctt tggtttgcat ctttgacaca gcgatggaac
    12481 tggcacaatt cgcatcgcag aggcgaattg tggatggact cagccgccgc gactgcgtag
    12541 aatccgctgc tcgatcaagc ctgtacgcgc ccgtagctca gttggataga gtgttggcct
    12601 ccgaagccaa aggtcagagg ttcaaatcct ctcgggcgcg ccattttccc tcccccctca
    12661 cgatttcacc ccgcgagacg ttcgttcgct gaactactcg ggttggggtc cggtccgtcc
    12721 tgcgcaaaga ggcaaacagc cagctgccaa gaggtggcgg gaaccgggca gatgtcgaga
    12781 ggatggccgg atcagccgtc gaatgcgaac agctcgtcga ggccgcagac agtgatgatc
    12841 tgccgcaatg ccccgggggg agactgaaag agcagtggct tctgttgccg cagtgcgtgg
    12901 cgctgccagc cgagcagcag cgagaccacg atgctgctcg gccgctccag tcgggccagc
    12961 gagattcgca ccggactggc tgaatcatcc agcagttgct cgccgcggcg acggcactcg
    13021 atgacattgt cgaagtcgag ttggccgatc agctgaatcg agccatcgct gtcgcggagg
    13081 agttcgaccg ccatcgcgtc agcctttcct ggtcactttg ttgacctcgt ccagctcggc
    13141 cgaccagcca tcgatcacct gatcgatgtt ctgcttctga tccatcgagc tggcaaactg
    13201 actgcggaag gtgaggccga ggttgatgcc attgacgatc acattctgca cccgccactg
    13261 tcgggcatcg tcgaggaaca tcgagtaggc gaccggatag atggtcccat cgtggccatg
    13321 gacctccatc atgacgctgg cctgacccgg ctcgtcgccc ggctgcggcc gatagggcag
    13381 caccacgatc ttctggttgt cgaagttcat gaaggccttg gcataggtct ggagcatccg
    13441 ctccttgaac accccggcga agcgcttgcg ctgcgcatcg ctggcctggc ggtaatattt
    13501 gcccatcact cccttggcaa tgcgatcgaa atccgatgcc ggcgtcatca gttgatcgat
    13561 ggcctgataa aagtgctgcg gttcgcggtc gaaacgggac ttctcctgca ggatcagcgc
    13621 gatcatgcgg tcggtgatct gctggatcac ctgatccggt gggggttcgg cggccaaaac
    13681 cggaccggac cagggtaaca gcagcaggag ggcgagcagc aggtgacgaa tcatcgcatt
    13741 gattccacgc tgaaaattgg agtgcggtct ggggcgggtc acttcgagcc gctcttgtcg
    13801 attgagctgg tgaggaactt gccgatcaga tcctcgagca ccaaggccga ctgggtgctc
    13861 ttgaagcgat tgccatcgct cagcatcttc tcttcggcac cgatcgagat gccgatgtac
    13921 tgctgtccga gcatgccctg ggtgaggatc atcgcagcgc tgtcgacggg caatgcgttg
    13981 acgctggcgt tgatctgcat ggtgacatgg ccgacatagc gctgctgatc gagggtgatg
    14041 gtggtcacgc tgccgatctc gacgccggcc acggtgaccg gggcacgcac cttgaggccg
    14101 ccgatgttct gaaagtcggc gtagagggtg taggtggcct cgggagcggt cagtgacagc
    14161 ccgctgaccc gcagcgccag acccgtcaag gccgcggcgc cgagcagcag aaagagtccg
    14221 accaggattt caagagtacg tgtctgcatg gctagaggtt cccgaacatg actgccgtca
    14281 gtatgaagtc gatccccaat actgccagcg aagagaagac cacggtcttg gtggtggccc
    14341 ggctgattcc ctcggaagtg gggaggcact catagccctg caccaccgcg atccagacca
    14401 ccacaaaagc aaaggccaga ctcttgagca ggccgttgac cacatcatca cggaaggaga
    14461 cattggcctg catggtcgac cagaaggtgc cggcatgcag tcccagccaa tcgaccgcca
    14521 ccagtttgcc gccccagacc ccgaccatgc agaagatcat ggtcaacaga ggcagcgaga
    14581 tgaagccggc cagcaggcgt ggtgcgatga tgcgccgcaa cgggtcgaca ccgatcatct
    14641 ccatgctggc caactgttcg gttgccttca tcaggccgat ctcggcggtc agcgctgaac
    14701 cggcacgtcc ggcgaacagc agtgcggtga ccaccggtgc cagttcccgg gtcaggctca
    14761 gggccaccag ttgccccact gccgaggtgg tgccgtagtc ggaaagaatg gtgtagccct
    14821 gcaaccccag caccatgccg atgaagagcc cggagaccag gatgatgacg agcgacagca
    14881 cgccgacgaa gtggatctgc ttcaccagca gcggcaggcc ctgcaggctg ggccagaccg
    14941 cgagcgaatg cagcagcagg ttgccggcac ggccccagat gaccaggaat tcgagcgtgg
    15001 cacggccgag ccgttgcagc agtgccatca ccatggctgc cccccgagca ggtcggttcg
    15061 atagtcgggg gccggatagt gaaacgcgat cgggccatcc ggcgaaccgg tcaggaactg
    15121 ccgcaccagc gcagagtcgc tgttcttaag ttcgtccgga cggccctgac cgatgatctt
    15181 gccgttggcg atcacgtaga tgtagtcggc aatctccatg gtttcgacca gatcgtggga
    15241 gacgatcacg ctggtgatgc ccagtgcatg attgagcagt ttgatcagtt cgaccaacac
    15301 ccccatcgag atcggatcct gtccggcaaa gggttcgtcg tacatgatca tcgccgggtc
    15361 gagcgcaatc gcccgcgcca gcgctgcccg gcgcgccatg ccgcctgaca gttcggccgg
    15421 catcaggtcg cgggcaccgc gcagacccac cgcatgcagt ttcatcagta ccaggtcgcg
    15481 aatcatctct tcgggcagcc gggtgtgggc gcgcagcgga aaggcgacat tctcatagag
    15541 cgaaaggtcg gtgaagaggg cgccgctctg gaacagcatg cccatcttgc gccgcacctc
    15601 gaacagccgg cggcggttca gtctggcgat gtcctcgcca tcgacacgga tggcgccggc
    15661 atcgggccgc agctgtgcgc cgatcagccg cagcagcgtg gtcttgccgc agccactcgg
    15721 tcccatgacg gcggtgatgg cgccgcggcg gatgtcgaaa tcgacattgt cgaagatcgc
    15781 acggctgccg cgacgaaagg tcagattgcg aatctcgacg aagttgtcgg tggtcctggc
    15841 catcggtatc gcgaaacaaa aagggctaga atgctctggc acgacacact ggctgtgtcg
    15901 tgccggcgga ggctttgaaa aagttttctc aattctagca gagggttgtc atggcgtctt
    15961 caactgccag gtgatgcaac tggtcagagg ctccatgtcg caccggtttt ccatccttac
    16021 catttttctg tcaatccgat cgattcagca tgaaaaccga ttaccggacc tttgactttc
    16081 tcggctctgc ccgccgcacc ctgacgctgg agagcgccgc gctgaccgcg ctggaggcgc
    16141 ggctcgacat ggcgttcgtg caggcctgcc tgctgatgct cgattgtcag ggacgggtcg
    16201 tcgtcaccgg catgggcaag tcgggccatg tcgccaacaa gatctcatcg accctggcca
    16261 gcaccgggac gccggcgttc ttcgtgcatc cggccgaagc cagccatggt gacctcggca
    16321 tgatcacccg gcaggatgtg gtgttggcgc tctccaactc cggcaccacc gccgaggtgc
    16381 tggcgatcgt gccgctgatc aaacggatgc aggcccgcct gatcagcatg accggcgatg
    16441 ccgcttcgcc actggccagg gctgccgagg tgcatctgga tgtggcggtg gcgcaggagg
    16501 cctgtccgct cggactggcc cccaccgcca gcaccaccac ggcgctggcg atgggcgatg
    16561 ccctggcggt ggcactgctc gaagcacgcg gtttcaccgc cgagcagttt gctttctccc
    16621 atcccggtgg cagccttggt cgccgcctgc tgctgaaggt cgaggatctg atgcatggcg
    16681 acgaatcagt gcccagggtc acggcctcga ccccgctgcg cgatgcgctg gccgaaatca
    16741 gccgctgtgg ctttggcctg accagtgtgg tcgatgggca gggccgcatg atcggcatct
    16801 tcaccgatgg cgatctgcgt cgcgtgctcg accgcggtct cgacgtgcgt gccacctcgg
    16861 tggccgaagt gatgacgccg aagggcgccg tggtccagcc ggagcaactg gccgtggatg
    16921 ccttgcagtg catggaggcg cggcagatca tggccctgat cgtcgtcgat gaagagcgcc
    16981 gtccggtcgg cgtgttgcac atgcacgatc tggtccgctc gggtgtggta tgatcgcgcg
    17041 ctgaatatct ggaagaaatc tccttgtccg acaagctgtt gcaaaaaatc gaacgaatcc
    17101 ggctgctggc gttggatgtc gatggcgtgc tcaccgatgg ccggctctac tttctgcccg
    17161 atggcggcga aatcaaggtg ttcaacagcc tcgatgggca tggcctgaag atgctgcaac
    17221 gcagtggagt cacggtggcg ctgatcaccg gacgcagttc gactgcggtg gcgcgccgtg
    17281 ctgccgaact cggcatcagt cacctccatc agggcagcga acagaagttg cctatattcg
    17341 atgggctgct ggagcagctt gggctcgact acagccaggc agcgttcatg ggcgatgacc
    17401 tgcccgatct gccgctgatc aggcgggccg cagtggggat gaccgtcgcc aatgcccacc
    17461 cgctggtgcg gcaaagcgcc gactggcaga gcaccctggc cggtggccag ggtgcggtgc
    17521 gcgaagcgtg cgacctgatc atgcagacgc agggtacgct ctgccggttg ctgcaagcct
    17581 acgagtgacc actcccatgc gtgccaacac ctggtggatc ggttgcattc tgctgctgtg
    17641 tggcgggctg ctgctgaagg tctcgctcga catggatgct tcccggcaac gtggcggtct
    17701 ggtcgatgac aaccagcccg attttttcat ggcccgggct gccacccgca tctacgatga
    17761 agagggccgt gtggcttacc tgctcagtgc cgagcgctgg agtcatgttc gcggcgagcc
    17821 tggcatatcg cttgcgcagc cgaatctgat cgcccatcga accgagtcat cgcagcgctg
    17881 gcaggcacag gccgagcgcg gctactgggt cgagagcgat ggtgtgttga ccctcgatca
    17941 ggccgtcact tttgagttgt gggcgcagca acggcggttg caacagctgc tcacgccgca
    18001 catgcagatc gactaccggc ggcgcaccgc ccataccgat gcgccggtca ggctgctggc
    18061 cgagcagggt gaaatgagtg gcaatggcat cgacatcgat ctggttgccg gcaaggccgt
    18121 gttgccgagt caggtgcggg ggcactatct tcccagtcaa ccaggagcat cgaagtgaaa
    18181 aaaaatctgc tttcgctccg gcgccttctg ctgctgtcac tgttgccatg gctgccctcc
    18241 acgctgctgg cgctcagtgg cgacagccgg cagccgatcg aaatcgtctc cgaccgcgcc
    18301 gaattcgatg acaacagcgc atcggcgcat tacatcggca atgtggtggt gacacagggt
    18361 tcgctgcgca tcgaggccga cgatctgctc ctgatcagtg acaagggcag tgtcaccgag
    18421 gtcaaggcca gcggcacccc ggcccgtttt catcagcagc tcgatggcca ggagccgttg
    18481 atgatggagg gtgaagccac ctcgatcagc taccatgcca agagccgcca gcttgaactg
    18541 aaaggcaccg ccaaactgcg gcagggtgag cggttcttct atggcgagcg catcgaatac
    18601 cagctggaga agcggttgat gcgcgccttt ggcggcagcg aggccaaggg ggagggcaag
    18661 cgggtccggt tgatcctgcc gccgcccaag gatgaggcga cgccatgaca gtggccgcca
    18721 gcgagcaggc acgggccgac agttgtctga acgtcagcca tctgtgcaag cgctaccaga
    18781 agcggcaggt ggtgcgtgat gtctcgttga cgctgcgcag cggcgagatc gttggtctgc
    18841 tggggcccaa cggtgccggc aagaccacct gcttctacat gatcatcggc ctggtgcgtg
    18901 gtgatggtgg caccatcacc ctcgatggtc aggagatcac ccatctgccg atgcatcgac
    18961 gggcacgccg aggcttgggc tatctgcccc aggagccctc ggtgttccgc cagatgtcgg
    19021 tggccgacaa catcctggcc gtgctcgaga cccgtgcccg attgagccgc tccgaacagc
    19081 gccagcggct cgatcagctg ctggatgagt tcaacatcgc ccacttgcgc aacgagctgg
    19141 gcatgagtct gtcgggcggt gagcgccggc gggtggagat tgcccgggcg ctggcggtgg
    19201 agccgcgctt catgctgctc gatgaaccct tcgccggcgt cgatccgatc tcgatgagcg
    19261 acatcaaggc catcgtgcgg cagttgcagc agcgttcgat cggcgtgctg atcaccgatc
    19321 acaacgtccg cgaaacgctc gatatctgtg aacgggccta catcgtcagt gatggcggca
    19381 tcattgccga gggtgatccc gcggctataa tgcagaacag gcaggttcgt gaggtttatc
    19441 tcggcaagga gttcagtctg tagtgcgcat catgcctcgt tttaccctcg gtcacgggct
    19501 ctgacatgaa accttcgctt cagctcaagc tgggtcagag cctgaccatg accccgcaac
    19561 tgcagcaagc gattcgtctt ctgcagcttt cgaccctcga tctccatcag gagattcagc
    19621 aggcgctcga ctccaacccg atgctcgaac tggtcgagga tgaagatgaa cggcgtgccg
    19681 atgagtcgat cgacgcctac ggtgagcaga cttcggccga agcggacgac agcggcgacc
    19741 tcgatggggc agagttcgac agtcacggcg atgatgagtg gagcgaggcg attcccgaag
    19801 atctgtcgat cgacaccagc tgggacgaga tctatcagag cagcggaacc ggttcgtcca
    19861 atccggagag cgatgaggat ttcgagtccc gcaacagcgt caccgagagc ctgcaggacc
    19921 acctgcgctg gcagatcaac ctgacccatc tcagtgatgt cgatcggctg atcgcgctga
    19981 atctcatcga tggcatcaat ccggatggga tgctcaccac ctcgatcgag gatgtacagc
    20041 tcagcctggc gcgcgatcag atcgaggtcg atctggatga gatcgaagcg gtgctgcatc
    20101 tggtgcagca gctcgatcca atcggtgtcg gcgcacgcac gctggccgaa tgtctgctgc
    20161 tgcaattgcg ccagtttccg gccgacaccc cttggcgcaa gcaggccgaa aagatcctga
    20221 ccgaccacat ggctgcgctc ggcagccgtg actacagcca gctgatgcgg ctgctcaagc
    20281 tgaccagcga ggagctgggc caggtggtgc agttgatcca gcagctcaat ccgcgtcccg
    20341 gcagcatcat ctccagcagc cagtcggagt acatcattcc cgatgtctac acccgcaagg
    20401 tgcgcggaga gtgggtggtc gagctcaatc cggaaagcat gccgcggttg cgggtcaacg
    20461 agcactatgc cgccatggtg cgccgcgccg acaatggtgc cgacaacacc tttctgcgca
    20521 acaacctgca ggaggcgcgc tggtttctga agagcctgac gagtcgcaac gagacgctgc
    20581 tgaaggtggc gcgcaagatt gtcgaggtgc agcgcggctt tctcgaccat ggcgccgagg
    20641 cgatgaaacc gctggtgctc tacgacattg ccgaggcggt cggcatgcat gagtcgacca
    20701 tctcccgcgt caccacccag aaattcctgc acacgccacg tggcattttc gagctgaaat
    20761 atttcttctc cagccatctg gagaccgatt cgggcgggga gtgctcatcc actgcgatcc
    20821 gcgccgtcat caaaaagctg atcagcgctg aaagtgcaaa aaaacccttg agtgacagca
    20881 aaatcaccgc catcctgtcg cagcagggca tcaaggtggc gcggcgtacc atcgccaagt
    20941 accgcgaatc gatgggcatt gccccctcca acgagcgcaa gcagttgccc tgatcgatgg
    21001 cggtcccgct gtcgccttcc acgtctcaca caacaggaac ccttcgcatg cagatcaatg
    21061 tcagtggcca ccacgtcgaa gtctcacccg cgctgcatga ttacgtcacc agcaagatga
    21121 accggatcga acgccatttc gaacagatca ccagcatcga tgtgatccta tcggtcgaga
    21181 agcagcggca gaaggccgag gcgaccatcc gcttttccgg tggcgagatc tttgccgaca
    21241 tcgagtcgga tgacatgtat gcggccatcg atgcgatggc cgacaagctc gatcggcaga
    21301 tgatcaagca caaggagaag tcgattggcc gccaccatgg tggttcgaat cgataaccgt
    21361 tcacatcccc gcaacgttga tgtcgtcgcg gtcggcggcg tcaacgccgg ttgatgcaac
    21421 catcctccca tcgatggaca cccgcacccg actcgtcatc atcagtggcc gttccggctc
    21481 gggcaagagc acggcgctgc atgtgctcga ggatctcggt ttctatgcca tcgacaacct
    21541 gccggccagt ctgctgaaca ccttggctgc gggggtcgct gccggtcagt atgccgagca
    21601 gcggcgcatc gcggtcagca tcgatgcccg caatgtgccg ggtgacctgt cgcggttgcc
    21661 ggagatcctc gaggaggtca gcagcatcac actgacgccg cagatcctgt ttctcgatgc
    21721 cgacgaccag acgctgttgc agcggttcag cgagacccgc cgccgccatc cgctgaccgg
    21781 cccccacctg ccgctgacgg aagcgatcgg tcgcgagcgt gaactgctgc ggccggtggc
    21841 ggcactggcc gatgtcaccc tcgacaccag ccgtctcagc ctgcatgacc tgcgcgatct
    21901 gatccgccgc cgtttctcga ccaccgaagg ggagaacttc tcgctgctgt ttgaatcctt
    21961 cggcttcaag aagggaacac cggccgatgc cgatttcgtc ttcgatgtcc gcgcgctgcc
    22021 caacccctac tggcacactg cgctgcgcgt ccatgatggg cgtgagccgc cggtgatcga
    22081 attcctggag gcccagcccg aagtgcggca gatgctcgat gacatcggca cttttctcga
    22141 ccgctggctg ccgcgctttc tggccagtgg tcgcagctat gtgaccgtgg ccctcggctg
    22201 caccggtggg cagcaccgct cggtctacct gtgtgaacgg ctggccgaac ggttctgcgg
    22261 tcgattcccc acggtcctgc tgcgccaccg cgagctgagc aacgcacgcg agccggtgca
    22321 ggtcgcggtg gcatcggcgc ctgaaaccgg ctaacctgcc ggccagttcg cagcgatcgc
    22381 tgccgcctgc tcctccccgt cgttcgatgc ggtgtcaccg gcttctcacc gggctgggca
    22441 gtgcccgttt tgtccggaat gcccgtcatg gccgactcca accaaggcgc ccgcgcgcgc
    22501 acccagaccc agcttcaggc gctcagcagc gcactggaga gcggcacctt cgtgcagatt
    22561 cggcgcatgt tgaacggtgt gccggcggtc gacgtggcgc ggctgctgga atcctcgcca
    22621 cccaacctgc gtgacctgct gtgggagctg gtcgacaagg agaaagaggg agagatcctg
    22681 cagcatctct ccgatgagtt gcaggggctg tttctgcggc ggatgaatgc cgagacggtc
    22741 gcctcggtgg tggagggctt cgacaccgac gacctggccg acctgctgca gcagttgccg
    22801 gagcgggtga tccgcgaagt gctcgatgca atggatgcgc aggaccgtca tcgggtcgag
    22861 cgggtgctgg cctatccgga agacagcgcc ggtggcctga tgaacaccga caccatcacc
    22921 gtgcggcccc gctttacgct cgacgtggtg ttccgctatc tgcgccgtca tgacagcctg
    22981 ccgccgatga ccgacaacat catcgtggtc aacagcgagg accgcttcat cggcctgctg
    23041 ccattgacca agatgctggt cagcgatccg tcgatcacgg tgcgcgagat catggtcacc
    23101 gatgtcgcgg cgattccggc ctccacctcg gccagcgagg tggccaagct gttcgagcgc
    23161 catgactggg tctcggcacc ggtggtcgat gagactggcc atctactcgg gcggatcacc
    23221 atcgatgacg tggttgacgt gatcatcgaa gcggctgatc agcaggtgat gagcatggcc
    23281 ggcctcggcg acgaggagtc caccttcatc gccccgttca aggccgcgac ccagcgcgcc
    23341 acctggctgg gcgccaacct gctgaccggc ttcatcgcgg ccggcgtggt ggggctcttc
    23401 gccgagacca tccatcaggt ggtggcgctg gcggtgttga tgccgatcat ccccagcatg
    23461 ggcggcgtgg ctggcagcca gacgctgacc ttgatgatcc gcggcatggc ccttggccac
    23521 atcggccgcg ccaaccgctt ctggctgttg cgtcaggagc tgctgatcgg gctgctgaat
    23581 ggcctgctgt gggccaccat cgtcggcgtg gccacggctt actggttcga tgaccggctg
    23641 ctgggggtga tccttgcagt ggcgatgctc tgcaacctgc tggcggcggc gttgaccggg
    23701 gtactgctgc cactgatgct gcgcaccttc ggcatcgacc cgattctggc cggatcggtg
    23761 ctgctgacca ccgtgaccga cgtggtgggc tttgtcgtgc tgctgggtct ggccagttgg
    23821 ttgctgctgt aaaaggagga ggacgggatg caggcgccag aagagaaacc aagcaagagt
    23881 gcgcgcaagc ggcggatgca tgaactgcaa cggctgggcg gcacgctgtt gcagttcagc
    23941 gatgcccagc tccggcggct gccgctgacc gaagcgctgc gtgaagcggt gcgcgaaggc
    24001 aagcggctca aggcgccggc ggcactgcaa cggcactgtc agtttctggg gcgcctgctc
    24061 gatgaagccg acaatgaagc actgttgatc gccatcgtga cactggaaca gggtacacag
    24121 cgggccgatc cctggcacca tcaggccgag cggctgcgcg acgcactgct ggcgggcgag
    24181 acggtgcact ttcaagcttg gctggtccag ctcgatcccg cggatcaggc gcaggtgaac
    24241 gctcaggccg agcgggcacg ccaagcagtg cgacgtgggc aggaggtggc gcgggagtcg
    24301 cgtcagctct accgactgct gcaccgcctg ctcgaggcag ccgccagcgc cagcgattga
    24361 ctaccccttc agttgccggt tgattcgctg gtaagcgctc ttgagctctt cgcccagcag
    24421 tttgaccgcc gcgccaattt cctggcccga ggcaaccccc tccgcgccgg cccgctgcaa
    24481 cttctggttg agctgctccc acttcttctc gctcttctgc cactcatcaa gcaggtcgag
    24541 cttgagcagg tgggcctgca gatgcagtgc atcccgttcg accttcaact gcgcggcaag
    24601 ctgtttcaga tcttcttgaa gcgtgctcat cgttggctcc ccatcgtata aaccggtaga
    24661 gaggatggat catacaggga ggccgggcaa cagtgttgcc acaggtcaag cccatggtcg
    24721 ttggttcgat tttgcggagc ggcccgactc accgcggtct ggtttcggac cctgcggagg
    24781 tcatcggttg cgggtgggtt gatcctgaaa caactcgacc agggtgatct tggggtcatt
    24841 gatcgggcca gtgacctggt aacgggtgct ggtgagctgg gcgagcggtt tttcgaacag
    24901 ctgtccggcg aggtaggcgg cggcggcggt gggcaaccca ccgatggcag ccacatacca
    24961 gggcagattg gtgccgatcg gcagcgtcat cgccatgtcg agatcgagat tctggctggc
    25021 aagatcgatg gtacctgcca gtttgaagcg cccgacagcc gactcgatgt tgatccggtc
    25081 atccagtgtc agaacattgt tggccagttt cattcggcca tcgacctgct gatagccaat
    25141 gctgccgccg ccgaagtcgg agaggttgag ctggagcttg ctgatcaggt tgttgaagtt
    25201 gaacaggctg aacagcccca gtgggctggt gccgccaccg gtctcgaagc gaccattccg
    25261 gaacagcagg cggtagtcgc cacgtgtccg ctccagcgcg aaatgggcgg gggagccggg
    25321 ccaggccaga ttgacatcga ttcgggccga ttggctgctg atgcccggca ccacgccgaa
    25381 atagcgcgcc agctgggcga tgtcgccccc ctccagcgta ctggtcagct gcgagcggtg
    25441 caggccaccg gtatcgcgct gccacaccat gagtgcgttg cgcagcgtga agccatcgat
    25501 ggtggcattg atctgttgcg cagtgatgcc ctgagcagtg ggccgcaact gaaaggccca
    25561 acttcccagc ggctgctcat cgatgtgaat ggcggcagcc tgaaaatcga gtgatggcag
    25621 cgtggcggga tcgaaattgg ccagtgggtc gttgtcggga cgattggagg cggtggtcag
    25681 cgtgagctgt tccagagcga tccggatcgg cgccgaatgg tcgagcgcca gctggagggt
    25741 gccacggacc ttgtcactgg caacccgcag atcgacccgc tcctgctgct gggaaatggt
    25801 cagatggacc tcattgagcg cctgcccgag aaagcgcagc tgtggcaggg tgaagtcgat
    25861 cttgccgaca tcggtgaacc agtcgacact cttctcgatg ccatcgatat gggccaggtg
    25921 ggcacggtaa aacgcgatcc actggtcgag atcgatcggt ggcagattgc cgcgcaacgg
    25981 attgtgggcc gagagtggcc gagcactctg actgctgccg agcaggatct gcccccggtc
    26041 gcggcctgcg gcatcaaagg tcagatcaaa accgatctct tcgttcagca gcagcgacag
    26101 cgattccagg ccggcggagt tgaagcgggt gagcaggaac agcggcaccg cctgttgcgg
    26161 cgccttcgac agcggtggtg gcaggctgct gccgagcccg accaggttgg attcgaaggt
    26221 gagttcgctg ccgtttccgc cggtcagtgg caactggagc tgggccacgt agggggcctc
    26281 gccttggaag aagtcgagcg gttgcagatc gagccagtcg gtcagatccg cgacgtcgac
    26341 gcgcccctcc tgggtcagca gcagggtggt atggccggtt gggtcactgc tctgttgcag
    26401 gcgcaccgtc accggctgcc cgagaaaccg cgccttgatc tggtcggcat gaactgcctg
    26461 atccgtggca aggcgcagcg cgccctccac ctggtcgtaa cgcagccggt aacggggtgc
    26521 ggcgacccga ccgttattgg tgcgcaggtc gagctgatag ccgattttcg gcttctctcc
    26581 gagggggaca tcgagtttca gccgcccctg caatggccca tgcagctcca gttcgccgag
    26641 ccaggggagc agctcctgac gcagcggcgt ggtcaacacc cactttttgg attcggccag
    26701 cgtactggtc agatctgact cgatcgagag atgatagtgc gggttacggt cgctccggcg
    26761 gccgacgacc ttgccttgcg tcagagcaag gttgccgctc ttgcccttga tgacctcgat
    26821 atcgaggttg gcatcgctga cccagaccga gccggccaac tcttcgagta tcggccaatc
    26881 cggctgaaag cgcagccgca tgtcgcggcc acgaaagaac agcagcgtcc gtttgccctg
    26941 ctcggggcca tggagccggc cctggtagac cacgctgccc tgctccagca gcccggtggt
    27001 caggctctgg cggatccagt cgatcagcct ggcattctcg gtcagggttg gcagaaactg
    27061 atctttctcc agcaggctgg ccgggttgat gccgatcgac agccccagtg tggccgcatc
    27121 gaggtcatgt cggggcagca gcaggcggaa cactccctgg gcgccgaagc ggtcggagcc
    27181 gagctggatg cggtcggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    27301 ggtcggagcc gagctggatg cggtcggaat caatttcgat cagttccggg gtcacctgcc
    27361 agaacagcgt accttgggcg gtgtcgattc gccagctttg cgcaaacagc tttggaaagt
    27421 gcaggtcgag ttgcttcgag tcgaactgca gcaggccgtg tccgccgcgg atgaacaatg
    27481 taccgtcgac gccactgacg gccggcgccc cctgccaggc ctgcatgctg aaggtggtga
    27541 gtcgggccga gagctcaagg tcgtcgaatg catcggtcgt cagccggggt gccagcagat
    27601 ggagtgcctc gatctgccca gtgggagcaa gctgttccag acgctgctgc caggcggaag
    27661 gcagcaggtt cgacccgatg agccagctcc gccatggccg cagatcgatc cgatcggcaa
    27721 acagctcgat ggccgcctga tcgggcgctc cggcaccgat caggtgcaga tgggtggtcc
    27781 ggggcgcttc attcggcagc agcaggcgcg gttcgagttc cagccgccag gccggccgtg
    27841 ccagcgaggc ggtgggtgtt gccgagctct cgtgctggta atgaaagccc agctggatga
    27901 cgggcgcatc ggctgaagcg gcagttgccg ttggcgccac ggcggcgctg atctctccgg
    27961 tcagcccgac cagttgcagg ccctcgagat cgccccacag ttcggcggtg gccgtgagat
    28021 gcgctggtgg ctcgaatcca cccagcagcc gcagccagcg ggcatcgatc ggctgttggg
    28081 gcaggcgcag atggaattcg ccggtgctgg gcagactgct tccgtcgaaa ccatgcagat
    28141 ggaggatcgc cgtggtgacg ggggccgttg catcgagctt gaagtcggcc gcgatgcgat
    28201 agccggctgc ggcctgctcc agatcgagct gtggcagcag cagcggcggc actgttgatg
    28261 tggcggccgc atcctgccac agcagcgtga catcatgcag ctgcaaccgt tcgggcagcc
    28321 caagcgggac gagcagtgcg tcgagcgggc gcgctctgcc gcctgcgccg gcatcgaccg
    28381 acagtgtcac gccgcgggcc gacagcgcga ccagcctggg ttggcgatgc cacagtgatt
    28441 gcagcagatg gagcgaaaat tccactgcat caccgcgcag tgaccagttc gctgtttcga
    28501 tttcgagtcc ctgcaggcga atcaccggcg ccaacccctc ccaatcgccg gtgatctgcg
    28561 tgaaggtgat ccggcattgg cagcggcggt tggcctcggc gctgagctga ctctggaact
    28621 catcgatcct gggcagcaga ttgcggccca cgccgaccac cacggctgcg cccagcagca
    28681 gcgcaaagcc gagccacagc agggcctgca gggccaaggt gatgaggcgc cagggcgtca
    28741 tgggggctgg agtcgggtca gcgcgaacag gcgggggcgg tggaaggtcg cgcatggccc
    28801 aagcctaaca tcgcgaaccg gatcgatcga gtgctggcca ggtcaccatg ccgcagcgtg
    28861 ccagcagcgc ctgggtttcg cgcagtggca atcccaccac ggcagagtag ctgccctcga
    28921 tgcgctcgac gaacggcgcc gccagtccct ggatcgcata gccgccggcc ttgtcgagcg
    28981 gttcaccgct ctcccagtag cgcgccagct ccgcgggatc gagcggacga aaccgcaccc
    29041 gagtggtgac cacggccgtg tggcaggcag tggcggtcac cacggcgacc gcgctgatga
    29101 ctcgatgcca gcggccgctc agttgcgtca tcatcgccat gaagtggtcg cggtcgcgcg
    29161 gtttgccgag aatctcgtcc gcctcggcca ccaccacggt gtcggcaccc agcaccggcg
    29221 catcagccag cggcaggctg tcgcgcccag tctcggcctt ggcctgtgcc agccgcagca
    29281 catgcgccgg ggatgactcg ccggccaagg gcgattcgtc gacattgaca gccagcagcc
    29341 ggcagggcac cgcgatctgc tccagcagcg cccgcctgcg cggcgaggcc gaggccagat
    29401 agacgaagcc gggcgcgtcc attcactcca cccggtagtg gcgccgcagg cggcgcagaa
    29461 gcggaaacag cagcggccag agcagcccgc tgctcaatgc actcaacagg cggtcgctgc
    29521 gccatggctg ctggctgatc agcagttgca tcaggctgag cagcagcagg tagagcgaaa
    29581 ccagcacgca gacgatcaac gcctgctgca cccggggaaa cagccgcaac tgctgatgca
    29641 ggctgaggtc gatgtagctc accaccatca gtgccaagcc atgttggccc agggtgacat
    29701 cgtgcagcac atcgagcggc agtcccagca gccagccgaa cagcagcccg atgcgttccg
    29761 gcagggccat ggtccagtag atcaggcaga gcagggccca agtgggacgc cagggttgcc
    29821 agtcggccgg cagcggcacg acctccagca gcagcgcgat gacgaaggtc agcagaatga
    29881 ccagccagtt gccgatcggg ctactgcgca tcgtcggtgg cctcggtcac ggagttctgt
    29941 ccgggttgtg gtgtggtcag aatcaccagc acatggcgac tgatcgccag ttgtgcggtc
    30001 ggtatcgccc tgatctgtgt gaaaggctgg ccggcattgc ggttcaccga ctccaccacc
    30061 ccgaccggat agttggccgg aaagcgcccg cccaagccgg aagtcaccag ttggtcgccc
    30121 acctcgatgt cgctggtctc gggcacgttg agcagctcga gatggctgtc gtccccggtg
    30181 ccgacggcga tggcccgcac tccgttgcgc agcacctgca ccggtacggc atggttctga
    30241 tcagtgatca gcagcacccg gctgtggtgt ggccgcaccg agatcacctg tcccatgatg
    30301 ccgctggcat cgagcagtgg ctgccccttc ctgatgccac tcttcgcgcc ccggtcgatg
    30361 atcacgaagt ggcgtgatcg gtcgctggag accccgatga tctcggcgat cagcgcattc
    30421 tcatcgaggt gggtggtgga attgagcagc tggcgcaggc ggatgttttc ggcctgcaac
    30481 gcagccatct tctgcgcctt ctgcgacagc agcagccgct cggttctgag tgcatcgttc
    30541 tcgcgtgcca actgctggcg gctgctgaga ctgtcgcggc tgccggcata gagccgcagt
    30601 ggccagtcgg ccagttcgat catcggcgcc agcgcccagt cgagccgttc gcgcagcaga
    30661 tcgagggcat ggtaatgggc atcggcgacc atcagcccga ccgacagcag gccgagcagc
    30721 agcagccgca ggccgaggcg ggggcgtttg acgaagatca gcttgatggc aatctcacct
    30781 cagcctgttc ggtcactgca gcgggccgac ccgatcggtc cgttcattcg ctggagagca
    30841 gctcgaaatt gctggcgtcc atcatctcca gtgcccgacc gccgccgcgg gcgacacagg
    30901 tcagcgggtc atcggccacc cgcaccggca ggccggtctc cttggagatc aactggtcga
    30961 gcccgcgcag cagcgcgcca ccgccggtca gcacgatgcc ggactcggcg atgtccgatg
    31021 ccagttcggg cggcgactgt tccaatgccc gcttgaccgc ctgcacgatg ccggagagcg
    31081 gctcctgcag cgcttcgaga atctcctcgc tgtcgagggt gaagctgcgc gggatgccct
    31141 cggccaggtt gcggccacgc acatcgatct cgctcgattc gacgctcgcg aaggcgatgc
    31201 cgatctcctg cttgatccgc tcggcggtgg catcgccgat caggctgcca tactggcgcc
    31261 gcacatgggt gatgatcgcc tcgtcgaagc ggtcgccgcc gatgcgcacc gaatcggaat
    31321 agacgatgcc gctgagcgag atgatggc
//
LOCUS       DAORCQ010000022        29254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_855_length_29254_cov_5.837529, whole genome shotgun sequence.
ACCESSION   DAORCQ010000022 DAORCQ010000000
VERSION     DAORCQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 29254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29254
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_855_length_29254_cov_5.837529"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKI23_03810"
     CDS             complement(<1..234)
                     /locus_tag="PKI23_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003204963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNN36100.1"
                     /translation="MAIRHFLTLADLTPTELRQVIGRAIELKRMQRRGFAHQPMIGKV
                     LGMIFEKSSTRTRVSFEAGMAQMGGHAIFLSPHD"
     gene            complement(258..1451)
                     /locus_tag="PKI23_03815"
     CDS             complement(258..1451)
                     /locus_tag="PKI23_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNN36101.1"
                     /translation="MHNVMNTYRRLPVEFTHGEGIWLHDSQGRRYLDALSGIAVCGLG
                     HAHPAVTQAIRLQAEKLLHVSNLFQIPNQERLAELLCKVSGLDTVFFCNSGAEANEAA
                     IKLARLHGHRRGIDNPAIVVMEGAFHGRTLATLSASGNRAIQAGFEPLVKGFVRAPFN
                     DLAAIDTIARNNHEVVAILVEPIQGESGIKLADANYLAELRQRCDQHGWLLMLDEVQS
                     GNGRSGSYFAYQNRGILPDVVTTAKGLGNGVPIGACLAGGAAATLFQPGNHGSTFGGN
                     PLATAAARAVVSTIDQQQLAANAGQIGERLLAGFRSELTGCDSVRDIRGEGLLLGIEL
                     SEPCAEFPSLCAAQGLIVNVTADRVIRLLPPLIMTPDEADQLIERLVRLIRTHAADER
                     TKPRN"
     gene            1646..3223
                     /locus_tag="PKI23_03820"
     CDS             1646..3223
                     /locus_tag="PKI23_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNN36102.1"
                     /translation="MNPVERALFISRIEALCEEMGSVLRRAAFSPNIKDRLDFSCALF
                     DHEGKLAAQAAHIPVHLGSMAHAMADVVASIDWQPGSMVMLNDPYLGGTHLPDVTLIA
                     PLFCPSGSCQSRLVGFVANRAHHAHIGAASAGSMPLSRTLAQEGVVIPPTRVIANGRW
                     LPAGLELIRSIDAGRASAATDPALFGDFAAQVSANRAGAERAAELLSALGPSGFSSAL
                     NEVNDYAGRLADSALAAIPQGEYHHIDWLDDDGQGQRRIPLEVRLQVEPGRIEVDFSG
                     SASQVEGNINCPLSVAAAAVYYVFRCLMPAQTPNCAGAFRSIMLHAPPGSLLNARPPA
                     AVAAGNVETSTRVVDLLLGALAQAVPGRIPAASHGSMNNVAMGSRGDIGAAWDYYETI
                     GGGMGGSPLAAGLSAVQTHMTNTLNTPVESLERHFPLRISRYAVRRGSGGAGLHRGGD
                     GLIREFLLLQPTEVTLLTERRTLQPWGLAGGEPGAAGRNLLNGQPLPAKCQFVAQAGD
                     RLTVMTPGGGGWGLAEV"
     gene            3300..5324
                     /locus_tag="PKI23_03825"
     CDS             3300..5324
                     /locus_tag="PKI23_03825"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003301397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNN36103.1"
                     /translation="MSSYDHLLAPLDLGFVRLKNRVLMGSMHTGLEEHPDGFRRMAAF
                     YGERARGEVGLIVTGGIAPHISSVGPRGAKLTTEEEAQRHQIITEAVHREGGLICMQI
                     LHTGRYAHHPGLIAPSPIRAPISQFSPRQVSSEEIEEQIEAFVHTAALARHAGYDGVE
                     VMGSEGYFINQFLAAHTNQRSDEWGGSFERRSRLPIEIIRRMRQRVGPDFIIIYRLSI
                     LDLIERGSSWEEVVQLARAIEAAGVTLINSGIGWHEARIPTIATMVPRAAFTWATAKL
                     KGEVKVPLVTSNRINTPEVAEAVLARGDADMVSMARPFLADSDFVRKARLGRSDEINT
                     CIACNQACLDHTFTGRLNSCLVNPRACAETEFDYRPTSTPKRVAVVGAGPAGLAFATT
                     AAERGHRVTLFEAADRIGGQFNLARRIPGKEEFNETLRYFGRRIELTGVDLRLNHRAQ
                     ASELIDGGYDLVLLASGIQPRTPEIPGIDHPKVVGYIDAIREPQRIGQRVAIIGAGGI
                     GFDVAELLVHQGASSSLDPLLFAAEWGIDTELKARGAVAGIEPRFAPAAREVVLLQRK
                     ADRFGSTLGKTTGWIHRINLEKRGVRMIAGVAYRQIDDAGLHIEVNGEPRLLDVDHVV
                     LCAGQEPLCSLQQPLEQAGIRVQLIGGAKLAVELDAKRAIDEGCRLAAAL"
     gene            complement(5352..6377)
                     /gene="rsgA"
                     /locus_tag="PKI23_03830"
     CDS             complement(5352..6377)
                     /gene="rsgA"
                     /locus_tag="PKI23_03830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HNN36104.1"
                     /translation="MSKRHLTQQQRRRIDRHQGDRIRRQTVDEALASQGDEQIGLVMV
                     HRGALLEVESRDADGHPLRVQCHQRANIEPIAVGDRVVWRRTGIAGEGVITALLPRSA
                     LLARPSSHGGLRPIAANLDLVVIVLAPQPAPIEILLDRYLVAVEQIGIDAMLVLNKCD
                     LLTSLPAADADSVRALLELYRGLGYPTLETSCHDHSGMAALTERLQRRTAILVGQSGV
                     GKSSLLKALLPEAEIALGAISAANLRGRHTTTTAQLYHLPQGGDLIDSPGVREFGLWH
                     LSPDAVWAGFRELRPLLGRCRFRDCQHQQEPDCALTAAIATGVMAPSRWQNLRRILAN
                     DGSDSGQ"
     gene            complement(6425..7069)
                     /locus_tag="PKI23_03835"
     CDS             complement(6425..7069)
                     /locus_tag="PKI23_03835"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNN36105.1"
                     /translation="MSIKVIGAGFGRTGTLSFKQALEQLGFNPCYHMLEVFNNPTHIP
                     QWQAAAEGRLPDWKALLNGYQAAVDWPVCTFWRELTEAFPQAKVVLTLRDPQKWYQSI
                     MSTIYQTLVAPSSTDESPQLTAHRAMTRQLILQNTFDGRLDDPTHAIAVYQRHIETVK
                     SSLSPRQLLVYEVAQGWEPLCEFLGCPVPDSPFPQVNSTEEFQTRMLNRRTLKL"
     gene            complement(7203..8402)
                     /locus_tag="PKI23_03840"
     CDS             complement(7203..8402)
                     /locus_tag="PKI23_03840"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002985.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNN36106.1"
                     /translation="MQFKDYPIDHLMEITPRPPLVMTHGEGSWLWDHTGRRYLDFVQG
                     WAVNALGHSPRCIVEALAAQAPQLITPSPAFYNEPALQLAALLTRESCLDQVFFTNSG
                     AEANEGAIKLARKWGRQHKGGAHEIITTLDSFHGRTLATMSASGKAGWDTMFAPQVPG
                     FPKAVLNDLSSVERLITAHTVAIMVEPVQGEGGVNCASPAFLQGLRELADQHRLLLIF
                     DEVQTGIGRLGTLFGYQHYGVEPDIMTLGKGLGGGVPLAALLAKRAACVFEHGEQGGT
                     YNGNPLMTAVGLAVVQRLLEPGFLPQVRAMGAHLSSRLTELCVELDLGGERGIGLLRA
                     LELKQEIGTQVVTAARDALEPDGLLLNAPRPSLLRFMPALNVTAAEIDRMIDGLRSAI
                     TLVHKRA"
     gene            complement(8419..9426)
                     /locus_tag="PKI23_03845"
     CDS             complement(8419..9426)
                     /locus_tag="PKI23_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNN36107.1"
                     /translation="MRILVTGSAGFIGSALSLRLLERGDEVIGLDNLNDYYDVQLKRD
                     RLARLTAHPNFTDLRISLEDRPAVDALFASHAPQRVVHLAAQAGVRYSITHPHTYVDA
                     NLVGFMNIIEGCRHHQVEHLVYASSSSVYGANTAMPFSIHHNVDHPVSLYAATKKANE
                     LMAHTYSHLYRLPTTGLRFFTVYGPWGRPDMALFIFTKAILAGEPIDVFSHGHHRRDF
                     TYIDDIVEGVIRTLDQVAAPNPVWSGDQPDPGTSLAPYRLYNIGSNRPVELLRYIEVL
                     EACLGKQANKRLLPMQPGDVPDTYADVDDLIRDVGYRPETPIEVGIERFVRWYRDYYR
                     I"
     gene            complement(9455..10741)
                     /gene="tviB"
                     /locus_tag="PKI23_03850"
     CDS             complement(9455..10741)
                     /gene="tviB"
                     /locus_tag="PKI23_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HNN36108.1"
                     /translation="MFNFVVEQANIAIIGLGYVGLPLAVEFGKRYPTIGFDINIDRIN
                     ELKNNHDSTLEVSDAELRGSSQLRFSNDPAPLQGANVYIVTVPTPIDRHRRPDLTPLQ
                     GASRTIGQVIKPGDVVIFESTVFPGATEEECIPLIEAASGLKYNQDFFAGYSPERINP
                     GDKSHRLPMIRKVTSGSTPQVADYVDQLYRSIITAGTHRASSIRVAEAAKVIENTQRD
                     LNIALVNELALIFSRLGIDTEEVLVAAGTKWNFLPFRPGLVGGHCISVDPYYLTHKAQ
                     EIGYHPEVILAGRRINDGMGAHVVEQVIRLMTRKRVPVVDSRVLVMGLAFKENCPDFR
                     NTRVVDIVEALQGYHVQVDVHDPWIDPHHLRAEYEGINLVDTPQPGQYDAIVVAVGHD
                     QFRTLGSAGMRRFGKPLHVLFDVKYLFPKEEVDGRL"
     gene            10926..12326
                     /locus_tag="PKI23_03855"
     CDS             10926..12326
                     /locus_tag="PKI23_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNN36109.1"
                     /translation="MTTIRIAKHHVHVSYLILFALELLMLAASVLIGAYFRFGEPMLI
                     EEEVAHPLWRALLFALVLMLSMLAMGVYQVRFREGVLGMMLRTASSFMAGGLALSILF
                     YLFPMFFLGRGILALSLGVAFVLLGLLRVAFFGLVDTEAMKSRVLVLGAGVRAKNILD
                     RLRRRADRKGFVIHGFVPMEGEVVEVPRTEVLGFAQPLVDYSRAHRIDEIVCAMDDRR
                     RAFPLDALYQCKLAGIAVLDVAAFFEREIGRIELDLLHPSNFIFSDGFDRSAFRAFSK
                     RLCDLLFAVLALPLLLPVMLLARVAIGLEEGFGAPVIYRQQRVGLDDKPFTLYKFRSM
                     HTDAEKHGAQFAQKNDSRITRVGAVIRKYRIDELPQIFNVLKGEMSFVGPRPERPEFV
                     SQFGEEIPHYHSRHRVKPGVTGWAQINYPYGDSAEDARQKLQYDLYYVKNNSLMLDFL
                     ILIRTCETVLFGNGAR"
     gene            complement(12323..13390)
                     /locus_tag="PKI23_03860"
     CDS             complement(12323..13390)
                     /locus_tag="PKI23_03860"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNN36110.1"
                     /translation="MSPSWLIRLCLQPEQALALSLTDWSLLIRQARAANLLALLADTL
                     DRAGLLEQLPEPPRHHLNSALTLVQRQRQSVLWEIERLEEALQPLEQPFVLLKGAAYV
                     AAELPLAAGRLLSDVDILVAEQELAHAEQLLAIHGWHGAHHHPYDQHYYRTWMHELPP
                     LLHLKRQSVLDVHHRILPRTCRNSPDPASLLADSQPLAGFDKARLPSNVDLLLHSAIH
                     LFHESELHHGLRDLFDLRQLFRLFGSQPRFWSQLLDRATQWRQRQALFHACRHVEGLL
                     DDELPTPVKQALADSAPRWPVLKMLDFCYRRALQPQHPSCAPTGTAVALAGLYLRGHW
                     LRMPPHLLLPHLLRKALRSED"
     gene            complement(13387..14238)
                     /locus_tag="PKI23_03865"
     CDS             complement(13387..14238)
                     /locus_tag="PKI23_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HNN36111.1"
                     /translation="MTTGPFVVTVRSPIAAVARSLLRLYADFALASPTAFSDFHLHVA
                     PPATLRRWIRPQVFFHFDQTIPFKPLPRSQAYPLFEWGFNYAISTTAHRYLIIHAAVV
                     ERGGRGLILPGPPGSGKSTLCATLVHSGWRLLSDELALIDLTSGLLAPIPRPVSLKND
                     SIELIRQRFADAVLSDPCHDTQKGTVAHLKPPTASVELAHRMVEPAWVVMPRYRSGSR
                     SRLRPVPKGEMLMQLAEQSFNYTILGARGFEVLADLVERCSSHRLIYSDLDIALDRLD
                     QLTGMTP"
     gene            complement(14280..14576)
                     /locus_tag="PKI23_03870"
     CDS             complement(14280..14576)
                     /locus_tag="PKI23_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HNN36112.1"
                     /translation="MSATGPLTFNHARFGQWRQWDDTAILYDSRSGDTHLLSGIAAVL
                     VGRLAGHSAGLSHDQLQAALQQFDLQSLPAVIDQLCLLGIVAPFSASLASPPCH"
     gene            complement(14593..15258)
                     /locus_tag="PKI23_03875"
     CDS             complement(14593..15258)
                     /locus_tag="PKI23_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36113.1"
                     /translation="MKSSKETPVKPDATPGQTSRSTLASPARRALALNSLKAAPVVVT
                     TLSARSALAAECWSGKMSGNLSHPNNGCVLGRSPGYYKTRVGPWGGEPGWPPGFDSGC
                     SPLDSNGSITQTCQTMFEDESLNRGGRVPGTRFGTVFTSAPGNLKKLTLMQVLWQKPG
                     SFAFHAIAAYFNALNFSSYVLKPDVVLWIANSILATGSFTDDRGTVWTESQMKAYLNT
                     TWT"
     gene            15630..17714
                     /gene="prsK"
                     /locus_tag="PKI23_03880"
     CDS             15630..17714
                     /gene="prsK"
                     /locus_tag="PKI23_03880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNN36114.1"
                     /translation="MPGESVDFSQVGYGCVAVVALVLMPLVASRWRDRLPRLALLLVL
                     ILSVGWAAALGSGEVSLRMVLALEVMRGFAWMVLLWRMMAAPANQDGWRLYLDRQRMR
                     PRLALGAAAYLLLGVTLMLWPELSDRLTARIRLGADGRVVYLLLLNVFGLLMVELFYR
                     NTRVEQRWGIKFLAIAIGALFAYDFALYTEALLFARINPTLFGARGFVNAALLPLIGV
                     TAARLGNKPVELFVSRRVVVHTTALMGAGLYLLVMASIGYYLRNYGGSWGGVIQILFL
                     SGAVVLLVALFFSGTLRARLRVFFAKNFFAHRYDYREAWLKLSRELAALGDFTTVTTG
                     GIKLLADLVESSAGTLWWQSEQEGYRCCGAWGMALESDARVDADDSLAHFLCASSWVV
                     ELPEYLRTPEMYRGLDLASWQQRYPQLWLVVPLMRQEALAGFVCLLKPRVQRAINWED
                     HDLLKTVGIQLANHIALVSASEELARARQFEAFNRLSAFIVHDLKNVVAQLSLVVTNA
                     QRYKHNPEFIDDALDTLANAVTKMNRMLGQLRQRAVEAPDPQRTELCQLAREVVALHR
                     HRQPVPTLSCQLESLMLRADRERLGRVMGHLLQNAQEATAPQGRVELSVETNAAGEAV
                     VRVSDTGCGMDQKFIDERLFRPFDTTKGNAGMGIGVFEVEQYIRSIGGRVEVQSEPGL
                     GTRFVLTMPLMH"
     gene            17779..19161
                     /gene="prsR"
                     /locus_tag="PKI23_03885"
     CDS             17779..19161
                     /gene="prsR"
                     /locus_tag="PKI23_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNN36115.1"
                     /translation="MSSSMELKKILVVEDDPGLQKQLKWSFDDYEVVVAGDREEAIAA
                     LRRHEPAVITLDLGLPPDPANASEGLATLAEILQLLPSAKVIVVTGNDERDNAVKAIG
                     MGAYDYYQKPIDPQVLGLIIQRAFHLHALEEENRRLQRGGDRSLTPLAGVIASSPQMV
                     KLCRTVEKLAPTDVTALLLGESGTGKEVLARALHTLSRRQRKPFVAVNCAAIPENLLE
                     SELFGYERGAFTGANKQTKGKIEYAQGGTFFLDELGDLPQSLQAKLLRFLQERTIERI
                     GGRELIPVDVRVVCATNKDLKRQVEAGQFREDLYYRVSEVTIDIPPLREREGDAVVIA
                     RLLLDRFAEQNSRALKGFTEEALSAIAGHDWPGNIRELENRIKRAVIMADGKWITADD
                     LELESQNREQPKLLNLREARERAETEAIRQALNAVEGNVSRAADTLGVARPTLYHMME
                     KYGITGTFAE"
     gene            19222..19635
                     /locus_tag="PKI23_03890"
     CDS             19222..19635
                     /locus_tag="PKI23_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36116.1"
                     /translation="MKRLLQKWLKRRQPPSAPSDQSGRGWIGVDLDGTLAHYDEWLGP
                     HHIGDPIEPMVARIRAWQASGVEVRIFTARASDYRHRRFVELWLERHELCGLKVTNQK
                     DYGMIELWDDRCVQIRTNSGEPVSSFFHQQEQTSS"
     gene            19632..20246
                     /gene="cysC"
                     /locus_tag="PKI23_03895"
     CDS             19632..20246
                     /gene="cysC"
                     /locus_tag="PKI23_03895"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNN36117.1"
                     /translation="MSDSPVSSNVVWHKSAVTRDLRQQQNQHRSMVLWFTGLSGSGKS
                     TLSHAVEQRLYERGCRTYVLDGDNVRHGLCGDLGFNEADRVENIRRVGEVSKLFLEAG
                     VIVLTAFISPFRSDRQRVRSILPHGDFIEIYCACPLEICEQRDVKGLYRRARAGEIKD
                     FTGISSPYEAPEKPGLVVDTGHVSLDACAQQVVDYIQPRLTAVV"
     gene            20277..21719
                     /locus_tag="PKI23_03900"
     CDS             20277..21719
                     /locus_tag="PKI23_03900"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNN36118.1"
                     /translation="MTSSMPKAPVLVPVILSGGAGTRLWPLSRELHPKQLLPVVNETT
                     LLQDTAQRLRGLAGSHPPIVICNDEHRFMVAEQLRMSGVGISAILLEPCGRNTAPAVA
                     MAAFEALQQWPNENPLLLVLPADHVVRQPDRFVQAVADGIAFAASELLVTFGIVPHAP
                     ETGYGYIKAGADLGSGTACRIERFVEKPDRETALGYLAEGSHYWNSGMFLFSARSFLE
                     ELERAAPLIFQSCAAAHQAVERDLDFSRIDRDIFAACPSDSIDYAVMEKTERGVVIPL
                     DAGWSDVGSWSSLWEISEGDADGNVILGDVLTSDTRNCYLRSEDRLIATIGLEDCIVV
                     DTADAVLVAHRDRVQEVKGIVSRIKADNRSEALLHRQVVRPWGSYEGIEQSERYQVKR
                     ITVNPGATLSLQMHYHRAEHWIVVKGTAEVTRGDEVFLLTENQSTFIPLGERHRLANP
                     GKVPLEIIEVQSGPYLGEDDIVRFKDVYGR"
     gene            21753..23057
                     /locus_tag="PKI23_03905"
     CDS             21753..23057
                     /locus_tag="PKI23_03905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNN36119.1"
                     /translation="MHVVMVGAGYVGLVSGACFAEFGANVVCVDVDARRVEALQQGRI
                     PIYEPGLDELVVRNVQAGRLGFSTDLSQHVPQADLVFIAVGTPSRRGDGHADLSYVHA
                     AAREIAGALAGYTVVVDKSTVPVGTARQVERIIREVNPQADFDVASNPEFLREGAAIK
                     DFMHPDRVVLGVATPRAEALLRELYRPLNLIETPILVTDWESAELIKYAANAFLATKI
                     SFINEMSMLCESVGADVHAVAKGMGLDGRIGRKFLHPGPGYGGSCFPKDTLALIRIAQ
                     EHDTPCRIVESVIEVNAAQKARMVKKIRDALGGELSGRQIAVLGLTFKPETDDMRDAP
                     ALSILPALLDKGAVVRAHDPQGMEEAKKYLPDTVRYYDDIYEMSEGVDAVVLLTEWNE
                     YRGIDLERLLQRMRGRVFLDLRNVYEPTAMRALGFDYTGVGR"
     gene            complement(23112..24767)
                     /gene="cysN"
                     /locus_tag="PKI23_03910"
     CDS             complement(23112..24767)
                     /gene="cysN"
                     /locus_tag="PKI23_03910"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNN36120.1"
                     /translation="MSHQSDLIATDIEAYLQQHEQKELLRLLTCGSVDDGKSTLIGRL
                     LHDSHMIYEDQLAAIKSDSVKSGTTGGDIDLALLVDGLQAEREQGITIDVAYRYFSTA
                     RRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARQGVLTQTRRHSFIVSLLGIRHV
                     VVAINKMDLVGFDPGRFEQIRQDYLDFAGRLNLPDIHFLPISALNGDNVVHRSTETPW
                     YHGKPLLELLEQIEISEDRNFDQFRLPVQYVNRPNLDFRGFSGTIASGVINRGDRLIA
                     LPSGKSSRVASIVTYDGELGTAWTPMAVTLTLEDEIDISRGDMLVRPEQLPQVSQRLS
                     AHLVWMHEDGLKVGREYYIKQSTKTVIGQVPQIHHRIDVNSLEQLPGGDTLSLNEIAL
                     CDLSVNAPLVFDAYDNNRGTGAFIFIDRLTNITVGAGMIVGARRDSDSDAELPRLGRS
                     ERENRFRQRTALVALTAANAQQLAEQLERRLCELGHNPLLIDQSNFPGATGHLAALKA
                     WGKAAGHLLITPLTEAAPLADLLLSEGSLGELLETLKQQEILE"
     gene            complement(24815..25726)
                     /gene="cysD"
                     /locus_tag="PKI23_03915"
     CDS             complement(24815..25726)
                     /gene="cysD"
                     /locus_tag="PKI23_03915"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNN36121.1"
                     /translation="MIPDHRLTHLKLLEAESIHILREVAAEFRKPVMLYSIGKDSAVM
                     LHLAMKAFYPGKPPFPLLHVDTTWKFHEMIEFRDQRARELGLELLVHINPEGLKMNIS
                     PFVHGSKKHTDVMKTQGLKQALDKYGFDAAFGGARRDEEKSRAKERVYSFRDKNHRWD
                     PKNQRPELWNLYNGRIDKGESIRVFPLSNWTELDIWQYIYLESIPIVPLYFAKERPVV
                     TRDGVDIMVDDDRMPLAEGETPQMKQVRFRTLGCYPLTGAIESTAASLTEIIQEMLLT
                     KSSERQGRVIDFDQAGSMEEKKREGYF"
     gene            complement(25822..28767)
                     /locus_tag="PKI23_03920"
     CDS             complement(25822..28767)
                     /locus_tag="PKI23_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNN36122.1"
                     /translation="MPNPSTTASGTLPHPSFELVRQHPIPALKLLLNEYRHGRTGARH
                     FHISADHDENVFLVAFPTVPMDSTGVAHILEHTSLCGSERYPVRDPFFMMIRRSLNTF
                     MNAFTSSDWTAYPFASQNRKDFFNLLDVYLDATFFSRLDPLDFAQEGHRLEFEQPDDP
                     STPLCYRGVVYNEMKGAMSSPTSLLWQTLTRHLYPTTTYHFNSGGEPDCIPQLSHAQL
                     LDFYRDHYHPSNAIFMTFGNLPVAELQQRFEQQALHRFDRREIMLPLHDEQRFHAPVK
                     VLEYYPTEAEADEVDRTHLVIGWLLGHCTDLDELLEARFLSAVLLDNSASPLLQLLET
                     SALGSAPSPLCGLEDGNRELLFVCGLEGSRPEHAEQLAADIMALLEKIADEGVPQPQI
                     EAVLHQLELTQREISGDGYPFGLQLLLAGLGAAVHGGDPAQLIDLDPALAKLRRHVED
                     PDYIKQLVRRLLLDNFHRVHLTLAPDPELAQRREQAVAAQLARIKAGLDAEQSRQIVE
                     QAAALKARQNRVDDESILPRVTLADVPATLQLAEPQRRDQLAGRTVTSYGQGTNGLLY
                     QQLVIDLPQFDESQLLELFLYASFLTDLGLGADDYLSIQQLQSQISGGYSAQISLRGK
                     AEDEQQLRGYLVLSGKALLRNHAPFVELLYRTLAEARFDELPRLRELIAQIRTSRERS
                     ITGSGHSLAMSAATAGMSPLARLGHRQSGLAGIRALKALDRTLDDPQRLAGLAERLQQ
                     IHQRLVAAPKQELLIAEPEQTAELLARLAHMQPPAAQGEFTPFALPPLREPTHALWLT
                     NTQVNFCARAYPTVPASHADAAALTVLGGFLRNGYLHRAIREQGGAYGGGAGQDSGIA
                     AFRFYSYRDPRLSETLADFDRAIDWVLGSQHELRQVDEAILGAIGSIDKPGSPAGEAK
                     RAFFNQLHGRDADHLQQVRQQILRVTLTDLQRVTERYLKPELASTAILTDSSRRDQAA
                     ALRLQVEEL"
     gene            complement(28850..>29254)
                     /locus_tag="PKI23_03925"
     CDS             complement(28850..>29254)
                     /locus_tag="PKI23_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNN36123.1"
                     /translation="GCTKCIQACPVDAIVGAAQWMHSVIASECSGCELCIEPCPVDCI
                     DLVRIEDETQRSWPQRRAQADQFRRRHQARTARLARLAAEQAERRQQTRNPAVAATAH
                     SSPTQADILAAVMRARARRAAQPPGSDPRQCD"
BASE COUNT         4999 a         8690 c        10021 g         5544 t
ORIGIN      
        1 gtcatgcggc gacagaaaga tcgcatggcc gcccatctgc gccatgccgg cctcgaagga
       61 gacgcgggta cgggtcgagg atttctcgaa gatcatcccc agcaccttgc cgatcatcgg
      121 ctggtgggca aagccgcgcc gctgcatccg cttcagttcg atggcgcgcc cgatcacttg
      181 gcgcaactcg gtgggagtca ggtcggcgag ggtcagaaag tgacggatgg ccatggtcct
      241 tgcttcgtga acggcgctca gttgcgcggt ttggtccgct catcggcggc atgggtcctg
      301 atcagccgca ccagtcgctc gatcaactga tcggcctcgt cgggggtcat gatcaatggc
      361 ggcagcaggc ggatcacccg atcggcggtg acattgacga tcagcccctg tgctgcgcag
      421 agcgagggga attcggcaca gggttcgctc agttcgatgc cgagcagcaa gccttcacca
      481 cggatgtcgc gcacagagtc gcaaccggtg agctcgctgc gaaagccggc cagcagccgc
      541 tcgccgatct gtccggcatt ggccgccagt tgctgctggt cgatggtgga gaccactgcc
      601 cgtgcggcgg cggtggccag cggattgccg ccgaaggtgg agccatggtt gcccggctgg
      661 aacagcgtgg cggcggcgcc accggcgaga caggcgccga tcggtacgcc gttgcccagc
      721 cccttggcgg tggtgaccac atcaggcagg atgccgcggt tctggtaggc gaagtagctg
      781 ccactgcggc cgttgccgct ctgcacctca tcgagcatca gcagccagcc atgctggtcg
      841 caacgctggc gcagttcggc cagatagttg gcatcggcca gcttgatgcc cgattcaccc
      901 tgaatcggct ccaccaggat ggcgaccacc tcatggttgt tgcgggcgat ggtgtcgatt
      961 gcagcgaggt cgttgaacgg ggcgcggaca aagcccttca ccagcggctc gaagccagcc
     1021 tggatcgccc ggttgcccga ggcgctcagc gtggccagcg tgcggccatg aaaggcgccc
     1081 tccatcacca cgatggcagg gttgtcgatg ccgcgccggt ggccatgcag gcgcgccagc
     1141 ttgatggccg cttcgttggc ttcggcgccg gagttgcaga aaaagaccgt gtcgagcccg
     1201 gagaccttgc agagcagctc ggccagtcgc tcctggttgg gaatctggaa cagattggag
     1261 acatgcagca gtttttcggc ctgcaggcga atggcctggg tgacggccgg atgggcatgg
     1321 cccagaccgc agacggcgat gccgctcagg gcatcgagat agcgacggcc ctggctgtca
     1381 tggagccaga tgccctcccc gtgggtgaac tccacgggga ggcggcggta ggtgttcatc
     1441 acgttgtgca tctgtcgttc gatccgaaaa cggccctcgg aaaaaaagag aaggcagcca
     1501 tggccgcctt gcatcgattc gcgatagtag tgattccatg ccggcttggc aacgaatcgg
     1561 gccggatcgg cagggcgtgg gcatgcggat gtcatcgtgc tgttccaagc agtagaatcg
     1621 gccagagcat catcagggca ctgcaatgaa tccggtcgag cgagcgctgt tcatcagtcg
     1681 catcgaagcg ctgtgcgagg agatggggtc ggtgctgcgg cgggcggcct tttcacccaa
     1741 catcaaggat cggctcgact tctcctgcgc actcttcgat cacgagggca agctggcggc
     1801 ccaggcggcc cacattccgg tccacctcgg cagcatggcc catgcgatgg ccgatgtcgt
     1861 tgccagcatc gactggcagc ccggttcgat ggtgatgctg aacgacccct atctgggcgg
     1921 tacccacctg cccgatgtca cgctgattgc gccgctgttc tgtcccagcg gcagttgcca
     1981 gagccgtctg gtcggttttg tcgccaaccg ggcccaccat gcccacatcg gtgcggcctc
     2041 cgccggttcg atgccgctgt cgcggacact ggcacaggag ggggtggtga ttccgccgac
     2101 ccgggtgatc gccaatgggc gctggctgcc ggctggcctc gaactgatcc gttcgatcga
     2161 tgccgggcga gcctccgcgg ccaccgaccc tgcactcttc ggtgactttg ccgcccaggt
     2221 cagcgccaac cgcgctggcg ccgagcgtgc cgcggagctg ctgagtgccc tcggacccag
     2281 tggtttctcc agcgcgctga atgaggtcaa cgactatgcc ggccgactgg ccgacagtgc
     2341 gctggcggcc attccccaag gtgaatacca ccacatcgac tggctggatg acgatggcca
     2401 ggggcagcgg cggattccgc tcgaggtgcg gttgcaggtg gagccggggc gcatcgaggt
     2461 cgatttcagt ggcagcgcca gtcaggtcga gggcaacatc aactgcccgc tgtcggtggc
     2521 cgcagcggcg gtctactatg tctttcgctg cctgatgccg gcgcagacgc ccaactgtgc
     2581 gggtgccttt cgcagcatca tgctgcatgc gccgcccggc tcgctgctca atgcccgccc
     2641 gccggcggcc gtggcggccg gcaatgtcga gacctcgacc cgggtggtcg acctgctgct
     2701 gggggcgctg gcccaggcag tgccggggcg gattcccgcc gccagccacg gcagcatgaa
     2761 caatgtcgcc atgggcagtc gcggcgacat cggtgccgcc tgggactatt acgaaaccat
     2821 cggtggcggc atgggcggca gtccgctggc ggccgggctc tcggcggtgc agacgcacat
     2881 gaccaacacc ctcaacacgc cggtggagag tctggagcgg cacttcccgc tgcggatcag
     2941 ccgctatgcg gtgcggcgcg gctcgggtgg cgccggtctg caccggggcg gcgatggcct
     3001 gatccgggag tttctgctgc tgcaaccgac cgaggtgacg ctgctgaccg agcggcgtac
     3061 gctgcaacct tgggggttgg ccggtggcga gccgggtgcc gccggccgca atctgctgaa
     3121 tggccagccg ctgccggcca agtgccagtt cgtcgcccag gccggagatc ggctgaccgt
     3181 gatgacgccg ggaggtggcg gctggggtct ggccgaagtt tgagtggcgt gcgcgcgccg
     3241 ccggacgcgt ggccggtgtt tgtcgaatcg atgatccata acaacaacga ggtgatgaga
     3301 tgagttccta tgaccacctg ctggcaccgc tcgatctggg ctttgtccgg ctgaagaacc
     3361 gggtactgat ggggtcgatg cacaccgggc tcgaggagca tcccgacggt tttcgccgca
     3421 tggcagcctt ttatggcgaa cgggcgcgcg gtgaggtggg attgatcgtc accggtggca
     3481 ttgcaccgca catcagcagt gtcgggccgc gtggcgccaa gctgaccacc gaggaggagg
     3541 cgcagcggca ccagatcatc accgaggcgg tgcaccgcga aggtggcctg atctgtatgc
     3601 agatcctgca taccgggcgc tatgcgcacc accccgggtt gatcgcccct tcgccgatcc
     3661 gggcgccgat cagccagttt tccccacggc aggtcagcag cgaagagatc gaggagcaga
     3721 tcgaggcgtt cgtccacacc gcggcgctgg cccgtcatgc cggctacgat ggcgtggagg
     3781 tgatgggctc cgagggctac ttcatcaacc agtttctggc agcgcacacc aaccagcgca
     3841 gcgacgagtg gggtggcagc ttcgagcggc gcagccggct gccgatcgag atcatccggc
     3901 gcatgcgcca acgggtgggg ccggacttca tcatcatcta ccggctgtcg atcctcgatc
     3961 tgatcgaacg gggcagcagt tgggaagagg tggtgcagct cgccagggcg atcgaggcgg
     4021 ccggcgtcac gctgatcaac agcggcattg gctggcacga agcgcgcatt ccgaccatcg
     4081 ccaccatggt gccgcgcgcc gccttcacct gggcgacggc gaagctgaag ggtgaggtga
     4141 aggtgccgct ggtcacctcc aaccgcatca acacccccga agtggccgag gcggtgctgg
     4201 cgcgcggtga tgccgacatg gtgtcgatgg cgcgcccctt tctggccgac agcgacttcg
     4261 tcaggaaggc gcggctggga cggtcggacg agatcaacac ctgcatcgcc tgcaaccagg
     4321 cctgtctcga ccacaccttc accggacggc tcaacagctg tctggtcaat ccgcgcgcct
     4381 gtgccgagac cgagttcgac tatcgaccga ccagcacgcc gaagcgggtc gcggtggtgg
     4441 gtgcgggccc cgctggcctg gcctttgcca ccaccgcggc cgagcgtggc caccgggtga
     4501 cgctgttcga ggcggcggat cgcatcggcg gccagttcaa tctggcccga cggattccgg
     4561 gcaaggagga gttcaatgaa acgctgcgct acttcggccg ccgcatcgag ctgaccggcg
     4621 tcgacctgag gctcaaccac cgagcgcagg cgagcgaatt gatcgatggc ggctatgatc
     4681 tggtgctgct ggccagcggc attcagccac gcacgccgga gatccccggc atcgaccatc
     4741 ccaaggtggt cggttacatc gatgccattc gtgagccgca acggatcggt cagcgggtgg
     4801 cgatcatcgg cgccggcggc atcggcttcg atgtcgccga gctgctggtt catcaggggg
     4861 cttcgagcag tctcgatccg ctgctgtttg ccgccgagtg gggcatcgac accgaattga
     4921 aggcacgcgg cgcggtggcg ggcatcgagc cgcgctttgc cccggcggcg cgggaggtgg
     4981 tgctgctgca gcgcaaggcg gaccgttttg gttccacgct gggcaaaacc accggctgga
     5041 tccatcgcat caatctggag aagcgcggcg tgcggatgat cgccggtgtc gcctatcgcc
     5101 agatcgatga tgccgggctg cacatcgagg tgaacggcga gccgaggctg ctcgatgtcg
     5161 accatgtcgt cctctgcgcc gggcaggagc cactgtgcag cctgcaacag ccgctggagc
     5221 aggctggcat cagggttcag ctgatcggcg gcgccaagct ggcggtggag ctcgatgcca
     5281 agcgggcgat tgatgagggt tgccggctgg cggctgcgct gtgaacgctg ggtgacaccg
     5341 ggcagcgcag ctcactggcc gctgtcgctg ccatcgttgg ccaggatgcg cctgaggttc
     5401 tgccaccggg agggtgccat cacgccggtc gcgatggccg ccgtcagcgc gcagtcgggc
     5461 tcctgctgat gctggcagtc gcgaaagcgg catcgcccca gcagtgggcg cagttcgcgg
     5521 aagcccgccc acaccgcatc cggcgacaaa tgccacagcc cgaactcgcg cacacccggc
     5581 gagtcgatca gatcgccgcc ctgtggcaga tggtaaagct gggccgtggt ggtggtgtga
     5641 cggccgcgca ggttggctgc ggagatcgcg ccgagggcaa tctctgcttc gggcaacagt
     5701 gccttcagca gcgaggattt gccgacgccg gactggccga ccaggatcgc ggttcgccgc
     5761 tgcaaccgct cggtcagtgc cgccatgccg ctgtggtcgt ggcaggaggt ttcgagggtg
     5821 ggataaccga ggccgcggta gagttcgagc agtgccctca ccgagtcggc gtcggcggct
     5881 ggcagtgagg tgagcaggtc acacttgttg agaaccagca tcgcatcgat gccaatctgc
     5941 tccaccgcga ccagatagcg gtcgagcaga atctcgatcg gcgccggctg cggtgccagc
     6001 acgatgacca ccagatcgag gttggcggcg atcggtcgca ggccgccgtg gctgctgggg
     6061 cgtgccagca gcgcgctgcg gggcagcagg gcggtgatca ccccttctcc cgcgatgccg
     6121 gtgcggcgcc agaccacccg gtctcccacc gcgatcggct cgatgttggc gcgctggtgg
     6181 cactgcaccc gcagcgggtg gccatccgcg tcgcggcttt cgacctcgag cagtgcacca
     6241 cggtgcacca tcaccaggcc gatctgctca tcgccctggc tggccagcgc ctcgtccacg
     6301 gtctgccgcc gaatccggtc accctggtga cggtcgatgc ggcggcgttg ctgctgggtc
     6361 agatggcgct tgctcacgtg gtcgaggcag aaatcacagg tgagtcaagg cggtgaaact
     6421 ccgttcaaag cttcagggtg cggcggttga gcatgcgggt ctggaactcc tcggtggagt
     6481 tgacctgcgg gaaggggctg tcgggcacag ggcacccgag gaattcgcac agcggctccc
     6541 agccttgcgc gacctcatac accagcagtt gccggggcga caggctcgac ttcaccgtct
     6601 cgatgtgacg ttgatagacc gcgatcgcgt gggtcgggtc atccagccgt ccgtcaaagg
     6661 tgttctgcag aatcagttgc cgggtcatcg cgcggtgagc ggtcagttgt ggcgactcgt
     6721 ctgtcgaact gggcgcgacc agcgtctggt agatggtgct catgatgctt tggtaccact
     6781 tctgcggatc gcgcagcgtc agcaccacct tggcctgcgg gaaggcctcg gtcagttcgc
     6841 gccagaaggt gcagaccggc cagtcgacgg cggcctgata gccgttcagc agcgccttcc
     6901 agtcgggcag tcgaccttcg gctgccgcct gccactgcgg aatgtgggtt ggattgttga
     6961 acacttcgag catgtggtag caggggttga aaccgagctg ctccagcgcc tgcttgaacg
     7021 agagggtgcc cgtgcgaccg aagccggcgc caatcacctt gatgctcatt ataaaatcct
     7081 ctttgcaata tgctgttttt acgatgttat ttttttgatt ctcaggcgga gttcagcttg
     7141 ggccgccgtc gcattctcgg cggtttgtca aaactgcgca aaccggtcgc cggggccatt
     7201 cgtcaggcgc gtttgtgcac cagggtgatg gcgctgcgca gtccatcgat catccggtcg
     7261 atctcggcag ccgtgacatt gagcgccggc atgaagcgca gcagtgacgg gcgtggcgcg
     7321 ttgagcagca gtccatcggg ttcgagcgca tcgcgcgcgg cagtcaccac ctgcgtgccg
     7381 atctcctgct tgagttccag ggcgcgcaac aggccgatgc cgcgctcgcc gcccagatcc
     7441 agttcgacac agagctcagt gagccgggag gagaggtgcg cccccatggc gcgcacctgc
     7501 ggcagaaaac caggctcgag cagcctctgc accaccgcca gacccaccgc agtcatcagt
     7561 ggattgccgt tgtaggtgcc gccctgctca ccatgttcga agacgcaggc agcgcgcttg
     7621 gccagcagtg ccgccagtgg cacgccgccg cccagcccct tgccgagggt catgatgtcc
     7681 ggttcgacgc cgtagtgctg gtagccgaac agcgttccca gccgaccgat gccggtctgg
     7741 acctcatcga agatcagcag caggcggtgc tggtcggcca gctcgcgcag cccctgcaga
     7801 aaggccgggc tggcgcagtt gacaccgcct tccccctgca ccggttccac catgatggcg
     7861 acggtgtgcg ctgtgatcag ccgctccacc gatgacagat cattcagcac ggccttggga
     7921 aaaccgggca cctggggtgc gaacatcgtg tcccagcccg ccttgccgga ggcggacatg
     7981 gtggccagcg tgcggccatg aaagctgtcg agggtggtga tgatctcgtg ggcaccgccc
     8041 ttgtgctgac gcccccattt gcgggccagc ttgatcgccc cttcgttggc ctcggcaccg
     8101 ctgttggtga agaagacctg atcgagacag gattcccggg tcagcagggc cgccaactgg
     8161 agggccggtt cgttgtagaa ggccgggctg ggagtgatca gctgcggtgc ctgggcagcg
     8221 agcgcctcga cgatgcagcg tggtgaatga cccagcgcat tgaccgccca gccctgcacg
     8281 aagtcgagat agcgccggcc ggtgtggtcc cacagccagg agccctcgcc atgggtcatc
     8341 accagcggtg gccgtggcgt gatctccatc aggtggtcga tcggataatc cttgaactgc
     8401 atggtggtga ttcctgggtc agatccgata gtagtcgcgg taccagcgca cgaagcgctc
     8461 gatgcccacc tcgatcggag tctcggggcg gtagccgaca tcccggatca agtcgtcgac
     8521 atcggcatag gtgtcgggca cgtcgccggg ctgcatcggc agcagccgct tgttcgcctg
     8581 cttgccgaga cacgcttcga gcacttcgat gtagcgcagc agttcgaccg gccggttgct
     8641 gccgatgttg tagaggcggt agggcgccag gctggtgccg gggtcgggct gatcccccga
     8701 ccagaccgga ttgggcgccg ccacctggtc gagcgtgcgg atcacccctt cgacgatgtc
     8761 atcgatgtag gtgaagtcac gccgatggtg gccgtggctg aagacgtcga tcggttcgcc
     8821 ggccaaaatc gccttggtga agatgaacag cgccatgtcg ggccggcccc aggggccata
     8881 gaccgtgaag aagcgcagtc cggtggtcgg cagccggtag agatggctgt aggtgtgcgc
     8941 catcaattcg ttggccttct tggtcgcggc atagaggctg accgggtggt cgacgttgtg
     9001 atggatcgaa aacggcatgg cggtgttggc gccatagacc gaactgctcg aggcatagac
     9061 cagatgttcg acctggtggt gccggcagcc ttcgatgatg ttcatgaagc cgaccaggtt
     9121 ggcatcgaca taggtgtgcg gatgggtgat cgagtaacgc acaccggcct gggccgccag
     9181 atgtaccacc cgctgcggcg cgtggctggc aaagagcgca tcgaccgccg gccgatcctc
     9241 caggctgatg cgcaggtcgg tgaaattggg gtgagcggtg agccgggcca gtcggtcccg
     9301 cttcaactgg acatcgtagt agtcgttgag gttgtcgagg ccgatcacct cgtcaccgcg
     9361 ctcgagcagc cgcaggctga gtgccgagcc aatgaagccg gcactgccgg tcaccagaat
     9421 acgcatgggg ttttcctgaa aaatactgcg agaatcagag gcggccgtcc acttcctcct
     9481 tggggaagag gtattttacg tcgaacagca catgaagtgg cttgccgaag cggcgcatgc
     9541 cggcgctgcc gagtgtgcgg aactgatcgt gtcccaccgc gacgacgatg gcgtcgtact
     9601 gccccggctg tggcgtgtcg accaggttga tgccctcata ttcggcacgc aggtggtgcg
     9661 gatcgatcca ggggtcatgc acatcgacct gaacgtgata gccctgcaat gcctcgacga
     9721 tgtcgaccac ccgggtgttg cgaaaatcag ggcagttctc cttgaaggcg agccccatca
     9781 ccagcacccg tgaatcgacc accggcaccc gcttgcgggt catcagccgg atcacctgct
     9841 cgacgacatg ggcacccatg ccatcattga tgcgccgccc ggccaggatc acctcggggt
     9901 ggtagccgat ctcctgcgcc ttgtgggtca ggtagtaggg gtcgacgctg atgcagtggc
     9961 cgccgaccag accggggcga aacggcagaa agttccattt ggtgccggcc gcgaccagca
    10021 cctcttcggt gtcgatgccg agccggctga agatcagcgc caattcgttg accagcgcga
    10081 tgttgaggtc gcgctgggtg ttttcgatca ccttggcggc ttcggccacc cggatgctgc
    10141 tggcgcggtg ggtgccggcc gtgatgatgc tgcggtagag ctggtcgaca tagtcggcca
    10201 cctgcggcgt cgaaccggag gtgaccttcc tgatcatcgg cagacggtgg ctcttgtcgc
    10261 ccggattgat ccgctccggg ctgtagccgg caaagaaatc ctggttgtat ttcagcccgg
    10321 aagccgcttc aatcaacggg atgcactcct cttcggtggc gcccggaaag accgtcgatt
    10381 cgaagatgac cacgtcaccc ggcttgatca cctggccgat ggtacggctg gcgccctgca
    10441 gtggcgtgag gtcggggcgg cggtggcgat cgatcggcgt cggaaccgtg acgatgtaga
    10501 cgttggcccc ttgcaacggg gccggatcgt tgctgaaacg caattgcgag ctgccacgca
    10561 actccgcgtc agacacttcc agtgtgctgt catggttgtt tttcaactca ttgatacgat
    10621 cgatattgat gtcaaaaccg atcgtcggat agcgtttgcc aaattcgacg gccagtggca
    10681 gacccacata gccgagaccg atgatggcaa tgttggcctg ctccacaacg aaattgaaca
    10741 tgtcgatgaa ccgctctgca aggaatggct gggggtgaaa gggctgaatg gagatcagcg
    10801 atacagggtg cgtcctgacg cagtcagctg ggcaacaggg caccagtcga atcttttcta
    10861 taatagaagc gtgatccttt cattgccatc cggcagggac tcgttgaaca ggagtcgaat
    10921 ccgcagtgac caccattcgt atcgccaagc atcatgtgca tgtctcctat ctgattctgt
    10981 ttgcgctgga actgctgatg ctggcggcct cggtgctgat cggtgcctat ttccgtttcg
    11041 gcgagccgat gctcatcgag gaggaggtgg cgcacccgtt gtggcgggcg ctcctgtttg
    11101 cgctggtcct gatgctgtca atgttggcga tgggggtcta tcaggtacgg tttcgcgaag
    11161 gagtgctcgg catgatgctg cgcaccgcca gcagcttcat ggcgggcggc cttgcgctgt
    11221 cgattctctt ttacctcttt cccatgtttt tcctcgggcg cggcattctg gcgctgtcgc
    11281 tgggggtggc cttcgtgctg ctggggttgc tgcgagtggc cttctttggt ctggtcgaca
    11341 ccgaggcgat gaagagccgg gtgctggtgc tgggtgccgg ggtccgggcc aagaacattc
    11401 tcgatcgcct gcgccgtcgc gcagacagaa agggctttgt cattcatgga ttcgtgccga
    11461 tggagggtga ggtggtcgag gtgccgcgca cagaagttct cggatttgcg cagccactgg
    11521 tcgactacag ccgggcgcat cggatcgatg agatcgtctg tgcaatggat gaccgtcggc
    11581 gcgcctttcc gctcgacgcg ctctatcaat gcaagctggc cggtatcgcc gtgctcgacg
    11641 tggctgcgtt cttcgagcgg gagattggcc gcatcgagct cgatctgctc caccccagca
    11701 acttcatttt ctctgatggc ttcgatcgca gcgcctttcg tgcattctcc aaacggctgt
    11761 gcgatctgct ctttgcggtg ctggcactgc cgctgttgct gccggtgatg ctgctcgccc
    11821 gggtggcgat cggtctggaa gagggcttcg gtgcgccggt gatctatcgg cagcagcgcg
    11881 tggggctgga cgacaagccc ttcacgctct acaaatttcg cagcatgcac accgatgccg
    11941 aaaaacatgg tgcccagttc gcccagaaaa acgacagccg aatcaccagg gtcggcgcgg
    12001 tgatccgcaa gtaccgcatc gatgagttgc cacagatctt caatgtgctc aagggcgaga
    12061 tgagctttgt cgggccacgg cccgaacggc cggagttcgt gtcgcagttt ggtgaggaga
    12121 ttccgcacta ccacagtcgg catcgggtca agccgggggt gactggctgg gcacagatca
    12181 actaccccta tggcgactcg gccgaggatg cccgccagaa gctgcaatat gacctttatt
    12241 atgtgaagaa caacagcctg atgctcgatt ttctgatatt gatcagaacc tgcgagaccg
    12301 tgctgttcgg caacggcgcg cgctaatcct cgctgcgcag cgccttgcgc agcaggtgtg
    12361 gcagcagcag gtgcggcggc atccgcagcc agtgtccgcg cagatagagt ccggccagcg
    12421 ccactgcggt gccagtgggt gcgcaactgg ggtgctgtgg ttgcaatgcg cggcggtagc
    12481 agaaatcgag catcttcagc actggccagc gcggtgcgct gtcggccagc gcctgcttca
    12541 ccggggtggg cagttcatca tccagcaacc cttcgacatg gcgacaggca tgaaagagtg
    12601 cctgtcgctg ccgccactgc gtggcccgat cgagcagttg cgaccagaat cgcggttgtg
    12661 acccgaacag tctgaacagt tgacgcaaat cgaacaggtc gcgcaggccg tggtgcagct
    12721 cgctttcatg gaacaggtgg atcgcgctgt gcagcagcag atcgacgttc gacggcaatc
    12781 gcgccttgtc gaaaccggcc agcggttgcg aatcggccag caggctggcg ggatcggggc
    12841 tgttgcggca ggttcggggc aggatgcggt ggtgcacatc cagcacgctc tgccgcttca
    12901 gatgcagcag cggcggcagc tcatgcatcc aggtgcggta gtagtgttga tcatagggat
    12961 ggtggtgggc accgtgccag ccatggatcg ccagcagctg ttcggcgtgg gccagctcct
    13021 gttcggccac cagaatgtcg acatcgctca gcaaccgtcc ggctgccagc ggcagctcgg
    13081 ccgcgacata ggcggcccct ttcagcagca cgaagggctg ttccagcggt tgcagcgcct
    13141 cttcgagccg ttcgatctcc cacagcaccg actgcctctg tcgctgcacc agcgtcaggg
    13201 ccgagttcag gtggtgccgt ggcggctccg gaagctgttc cagcagtccg gcgcggtcca
    13261 gcgtgtcggc cagcagcgcc agcaggttgg ccgcgcgcgc ctgccggatc agcaggctcc
    13321 agtcggtcag gctcaaagcg agcgcctgct cgggttgcag gcagagtctg atcagccagg
    13381 acgggctcat ggcgtcatcc cggtcagttg gtcgagtctg tcgagggcga tgtcgaggtc
    13441 ggagtagatc agccgatggc tgctgcaacg ctcgaccaga tcagccagca cctcaaagcc
    13501 acgggcaccc agaatggtgt agttgaagct ctgctcggcc agttgcatca gcatctcgcc
    13561 cttcggcact ggccgcagcc ggctgcgcga cccgctgcgg tagcgcggca tcaccaccca
    13621 ggccggctcg accatgcggt gtgccagctc cacgctggcg gtcggtggtt tgagatgggc
    13681 caccgtgccc ttctgggtgt catggcaggg gtcgctcagc accgcatcgg caaagcgctg
    13741 tcggatcagc tcgatcgagt cgttcttgag gctgaccggc cgtggaatcg gtgccagcag
    13801 tcctgaggtc aggtcgatca gcgccagctc gtcggagagc agccgccagc cggagtggac
    13861 cagggtcgcg cagagggtgc tcttgcccga gccgggcggc ccgggcagga taagaccgcg
    13921 gccaccgcgc tccaccacgg cggcatggat gatcagatag cggtgggcgg tggtcgagat
    13981 ggcatagttg aaaccccatt cgaacagcgg ataggcctga ctgcgcggca gcggcttgaa
    14041 cgggatggtc tggtcgaagt ggaagaacac ctgtggccga atccagcggc gcagcgtggc
    14101 gggcggcgcg acatgcaggt ggaagtcgct gaaggcggtt gggctggcca gtgcaaagtc
    14161 ggcatagagc cgcagcaggc ttcgcgccac cgcagcgatg ggcgagcgca ccgtcaccac
    14221 gaacggaccg gtggtcagcg acagacccgc cggccggtgc agttgccgcc ggatctcggt
    14281 cagtgacagg gtggcgaggc gaggctggct gagaatggcg cgacgattcc gagcagacag
    14341 agttgatcga tcaccgccgg caggctttgc aggtcgaact gctgcaaggc tgcctgcaac
    14401 tgatcatggc tgagcccggc agagtgcccg gcaagccggc cgaccagtac agcggcgatg
    14461 ccgctgagca ggtgggtgtc gcccgaacgg ctgtcataca gaatagccgt gtcatcccac
    14521 tgtcgccact ggccaaagcg ggcgtggttg aaggtcaacg ggccagtggc tgacatcgtg
    14581 cggcggcggg ggctaggtcc aggtggtgtt cagataggct ttcatctgac tctccgtcca
    14641 caccgtgccg cggtcatcgg tgaagctgcc ggtggccagg atcgaattgg caatccagag
    14701 cacgacatcg ggcttcagca catagctgct gaagttcagc gcattgaagt aggcggcaat
    14761 ggcatgaaag gcaaaggagc ctggtttttg ccacaaaacc tgcatcaggg tcagtttttt
    14821 cagattgccg ggagcggatg tgaacaccgt gccgaagcgg gtgcctggca ccctgccgcc
    14881 tcggttgaga gattcgtcct caaacatcgt ctggcaggtt tgggtaatac tgccgttgga
    14941 atccagtggc gaacagccgg agtcgaaacc gggaggccag ccgggttcgc caccccaggg
    15001 cccgaccctg gttttgtaat aaccggggct gcggcccagc acgcagccgt tgttggggtg
    15061 cgacaggttg cccgacatct tgccggacca gcattcggcg gccagtgccg agcgcgcgct
    15121 cagcgtggtc accaccaccg gggccgcctt gaggctgttg agtgccaggg cgcgccgcgc
    15181 aggtgaggcc agcgtcgaac ggctggtttg accgggtgtg gcgtctggct tgactggggt
    15241 ctctttgctg gatttcatca tggtgtccgt ttgatcgctc ggtgaggccg cgggcgccgt
    15301 tgcaaatctg tttctactat accaaaagcg aaccagaaga atcagcgggc agtcggtgac
    15361 tgggttcgtt cttcaaatac aagaatcatg cctttttttg gatattcatg tttatcagca
    15421 aggtagagca gatgtgtcct gcgtgcaaaa atcacccatg ccgactttgt aaaaaatatg
    15481 gacactcgtg tcccattgcg caagggcagg gtctgtcgat cagcggcagc cggagtgagc
    15541 caccaatcgc cacgccactg tcgcgtgtgg agcatcggcc ccgcagttca acgcttttgt
    15601 aaacctgaac cgatagagtg cgtgtcgtca tgccgggaga gtctgtggat ttttcgcaag
    15661 tcggatatgg ttgtgtggct gtggtggcgc tggtgttgat gccactggtg gccagccgct
    15721 ggcgtgaccg gctgccacga ttggcgctgt tgctggtgct gatcctatcg gtcggttggg
    15781 ccgccgcgct gggctcgggt gaagtttcgc tgcggatggt tctggcgctg gaggtgatgc
    15841 gcggatttgc ctggatggtg ctgctgtggc gcatgatggc ggccccggcc aaccaggatg
    15901 gctggcgcct ctatctggat cggcagcgga tgcgtccgcg actggcgctg ggtgctgccg
    15961 cttacctgct gcttggggtc acgctgatgc tgtggccgga gctgagtgat cggttgaccg
    16021 cacggattcg ccttggcgcg gatggccggg tggtctatct tctgctgttg aacgtgttcg
    16081 gtctgttgat ggtcgagctg ttctaccgca acacccgggt cgagcagcgc tggggcatca
    16141 agtttctggc gattgcgatc ggtgcgctct tcgcctatga cttcgcgctc tacaccgagg
    16201 cgctgctgtt tgcgcggatc aatccgacgc tctttggtgc ccgtggcttc gtcaatgccg
    16261 cgctgttgcc gctgattggc gtgaccgccg cgcgcctcgg caacaagccg gtggagctgt
    16321 tcgtctctcg cagagtcgtg gtgcacacca cggcgttgat gggtgccggg ctctatctgc
    16381 tggtcatggc gtcgatcggc tactacctgc gcaactacgg tggcagttgg ggcggagtca
    16441 tccagattct ttttctcagc ggcgcggtgg tgctgctggt cgcgctgttc ttctccggca
    16501 cgctgcgtgc ccggctccgg gtcttttttg ccaagaactt ctttgcccac cgctacgact
    16561 accgggaggc gtggctgaag ttgagccggg aactggctgc gctgggtgat ttcaccacgg
    16621 tgacgaccgg cggcatcaag ctgctggccg acctggtcga gagctcggcc ggcaccctct
    16681 ggtggcagag tgagcaggag ggctaccgct gctgtggtgc ctggggaatg gcgctggaga
    16741 gtgacgcacg ggtcgatgcc gatgattcac tggcacattt tctctgcgcg tcctcctggg
    16801 tggtcgagtt gccggaatac ctcagaacgc cagagatgta ccgcggcctc gatcttgcgt
    16861 cgtggcagca gcgctacccg caactgtggc tggtggtgcc gctgatgcga caggaggcgc
    16921 tggctggttt cgtctgcctg ctgaagccgc gggtgcagcg tgcgatcaac tgggaagacc
    16981 atgacctgct gaagaccgtc ggcatccagt tggccaacca cattgccctg gtcagtgcca
    17041 gcgaggagct ggcgcgcgct cggcagttcg aggcgttcaa tcggctctcg gccttcatcg
    17101 tccatgatct gaagaatgtc gtggcgcaac tgtcactggt ggtcaccaat gcgcagcgtt
    17161 acaagcacaa tccggagttt attgacgacg cactcgatac gctggccaat gcagtgacta
    17221 aaatgaaccg tatgctgggc cagttgcggc agcgggccgt ggaagcgccc gatcctcagc
    17281 gcaccgagtt gtgtcaactg gcgcgcgagg tcgtcgccct gcatcgacac cggcaaccgg
    17341 ttccgacgct gagctgccag ctggagtcgc tgatgctccg tgccgaccgc gagcggctgg
    17401 gtcgggtgat gggccatctg ctgcagaacg cgcaggaggc cactgcgcca cagggtcggg
    17461 tcgagttgag tgtcgagacc aacgcagcag gagaggccgt ggtgcgggtg agtgacaccg
    17521 gttgcggcat ggaccagaag ttcatcgatg aacggctgtt ccggcccttc gacaccacca
    17581 agggcaatgc cggcatgggc atcggggtgt tcgaggtcga gcagtacatt cgcagcattg
    17641 gtgggcgggt cgaggtgcag agtgaacccg gcctgggcac ccggttcgtg ctgacgatgc
    17701 cgttgatgca ttgagtcgtt ttggcaaagg gtgctgtgtt gcagctcctt aaaattcaaa
    17761 gagattggta aatcaggcat gtccagttca atggaactga aaaaaatatt ggtggtcgag
    17821 gatgatccgg gtctgcagaa gcagctcaaa tggtcgttcg atgactatga ggtggtggtt
    17881 gcgggcgatc gggaagaggc gatcgccgcg ctgcgccgcc atgagccggc agtgatcacg
    17941 ctggacctcg gtctgccacc cgatccggcc aatgccagcg aggggctggc gacactggcc
    18001 gagattctgc agctgctgcc cagtgccaag gtgatcgtgg tgaccggcaa cgatgagcgt
    18061 gacaacgcgg tcaaggcgat cggcatgggc gcctatgact actaccagaa gccgatcgat
    18121 ccgcaggtgc tggggctgat cattcagcgg gcctttcatc tgcatgcgct ggaggaggag
    18181 aaccggcggc tgcaacgcgg gggtgaccgg tcattgacac cactggccgg ggtgattgcc
    18241 agcagtccgc agatggtcaa actctgccgc acggtcgaaa aactggcgcc gaccgatgtc
    18301 acggccctgt tgctgggcga gagcggcacc ggcaaggagg tgctggcgcg cgccctgcat
    18361 accctaagcc ggcgccagcg caaacccttc gtggcggtca actgcgccgc gattccggag
    18421 aacctgctgg agagtgagct gttcggctat gagcgaggcg ccttcaccgg tgcgaacaag
    18481 cagaccaagg gcaagatcga atatgcccag ggcggtacct tttttctcga tgaactcggt
    18541 gacttgccgc agtcgttgca ggccaagctg ctgcgtttct tgcaagagag aaccatcgag
    18601 cgcatcggcg gacgcgagct gattccggtg gatgtccggg tggtctgcgc caccaacaag
    18661 gatctcaagc gacaggtcga agcaggacaa ttccgtgaag atctctacta ccgggtcagt
    18721 gaagtgacca ttgacattcc tccgttgcgt gagcgtgaag gtgatgcggt ggtgattgcg
    18781 cgcctgctgc tcgaccgttt tgccgaacag aacagccgcg ccctgaaggg cttcaccgag
    18841 gaggcattga gcgccattgc cggtcacgac tggccaggca acatccgtga actggagaac
    18901 cgcatcaagc gggcggtgat catggccgat ggcaaatgga tcaccgccga tgatctggag
    18961 ctggagagcc aaaaccgtga gcaaccgaag ttgctcaacc tgcgcgaggc gcgtgagcgg
    19021 gccgagaccg aagccatccg ccaggccctc aatgccgtcg agggcaatgt cagcagggcg
    19081 gccgacacct tgggtgtggc gcgtcccacc ctctatcaca tgatggaaaa gtatggaatc
    19141 accggaactt ttgccgagtg agacgccttt cgatcggact gccagcaccc cttggcaacc
    19201 cggtggatgg ccgccaggac catgaaacgc ctgcttcaga agtggctgaa gcgccgacag
    19261 ccaccgtccg cacccagtga ccagagcggg cgcggctgga ttggtgtcga cctcgatggc
    19321 acgctggccc actatgatga gtggctgggt ccgcaccaca tcggtgatcc gatcgagccg
    19381 atggtggcgc gaatccgcgc ctggcaggcc agcggtgtcg aggtacgcat cttcaccgcc
    19441 cgcgccagcg attaccgcca ccggcgtttt gtcgagctgt ggctggagcg gcacgagctc
    19501 tgcggcctca aggtcaccaa ccagaaggat tacggcatga tcgagctgtg ggacgatcgc
    19561 tgcgtgcaga ttcgcaccaa cagtggcgag ccggtgtcat ctttttttca tcagcaggag
    19621 caaacctcgt catgagtgat tccccggtca gcagcaacgt cgtttggcac aagtcggcgg
    19681 tgacccgcga cctgcgtcag cagcagaacc agcaccgcag catggtgctg tggttcaccg
    19741 gtctttccgg ctccggcaag tcgacgctgt cgcatgcggt ggagcagcgg ctgtacgagc
    19801 gcggctgtcg cacctatgta ctcgatggcg acaatgtgcg ccacgggctt tgcggcgacc
    19861 tcggtttcaa tgaggccgat cgggtcgaga acatccggcg ggtcggggag gtctccaagc
    19921 tcttcctcga ggccggtgtg atcgtgctga ctgcattcat ctcgccgttt cgcagcgacc
    19981 gccagcgggt gcgcagcatc ctgccgcatg gtgatttcat cgagatctac tgtgcctgtc
    20041 cgctggagat ctgcgaacag cgcgatgtga agggactcta ccggcgtgcc cgggctgggg
    20101 agatcaagga tttcaccggc atttcctcgc cctacgaagc gccggaaaaa ccagggctgg
    20161 tggtcgacac cggccacgtt tcgctcgatg cgtgcgcaca gcaggtcgtc gactacatcc
    20221 aaccgcgtct gaccgctgtg gtctgaccct ctttttcatc tcaagagcag tcatcgatga
    20281 catcatccat gccgaaggcg ccggtgctgg tgccagtgat tctgtcgggc ggggcgggca
    20341 cacgactgtg gccgttgtcg cgcgaactcc atcccaaaca gttgctgccg gtggtcaacg
    20401 agaccacgct gctgcaggac accgcgcagc ggctgcgtgg actggccggc agccatccgc
    20461 cgatcgtcat ctgcaatgac gaacaccgct tcatggtggc ggagcagttg cgcatgagcg
    20521 gtgtcggcat ctcggccatc ctgctcgaac cctgtggccg caacaccgcc ccggccgtgg
    20581 cgatggctgc cttcgaagcg ctgcagcagt ggcccaacga aaatccgctg ctgctggtgc
    20641 tgccggccga ccatgtggtc aggcagcccg accgtttcgt gcaggccgtc gccgacggca
    20701 ttgcgtttgc ggccagtgag ctgctggtca ccttcggcat cgtgccccat gcaccggaga
    20761 ccggttacgg ttacatcaag gccggtgccg accttggcag cggcacggcc tgccgcatcg
    20821 agcgtttcgt cgagaaaccc gaccgcgaaa cggcgctcgg ctacctggcc gaaggcagcc
    20881 actactggaa cagcggcatg ttcctgttct cggcacgcag ttttctggag gagctggaac
    20941 gggcggcacc gctgatcttt cagagctgtg ccgccgcgca tcaggcggtc gagcgcgatc
    21001 tcgacttcag ccgcatcgac cgcgacatct ttgccgcctg ccccagtgat tcgatcgact
    21061 atgcggtgat ggagaagacc gagcgcggtg tggtgatccc gctcgatgcc ggctggagtg
    21121 atgtcggctc ctggtcgtca ctgtgggaga tcagtgaggg cgatgccgac ggcaatgtga
    21181 ttctgggtga tgtgctgacc agcgacaccc gcaactgcta tctgcgctcc gaagaccggc
    21241 tgatcgccac cattggcctg gaggattgca tcgtggtcga caccgccgat gcggtgctgg
    21301 tggcgcaccg tgatcgggtg caggaggtca agggcatcgt cagccgcatc aaggccgaca
    21361 accgctccga ggcgctgttg caccggcagg tggtccgtcc atggggcagc tacgagggca
    21421 tcgagcagtc cgagcgttat caggtcaagc gcatcacggt caatccgggc gcgaccctgt
    21481 cgttgcagat gcactatcac cgtgccgagc actggatcgt ggtcaagggc actgccgagg
    21541 tgacgcgggg cgacgaggtg ttcctgctca ccgaaaacca gtccaccttc attccgctgg
    21601 gagagcgcca tcggctcgcc aacccgggca aggtgccgct cgaaatcatc gaggtgcagt
    21661 ccgggcccta cctgggcgag gatgacatcg tccgcttcaa ggacgtctat ggccgctgag
    21721 cgctcagccg cttgcagtaa caggagaaga gcatgcatgt cgtgatggtg ggtgccggct
    21781 atgtggggct ggtatcaggt gcctgttttg ccgagtttgg cgccaatgtg gtctgcgtcg
    21841 atgtcgatgc ccggcgcgtc gaggcgttgc agcagggccg gattccgatc tatgagccgg
    21901 gtctggatga gctggtggtg cgcaatgtcc aggccggacg gctcggcttc tcgaccgatc
    21961 tgtcgcagca tgttccgcag gccgatctgg ttttcatcgc ggtcggcacg ccaagccggc
    22021 gcggcgacgg ccatgccgac ctcagttatg tgcatgccgc agcgcgggag attgccggcg
    22081 cgctggcggg ttacaccgtg gtggtggaca aatcgacggt gccggtcggc acggcgcgtc
    22141 aggtggagcg gatcatccgc gaggtcaatc cgcaggccga tttcgatgtc gcttccaacc
    22201 cggagtttct gcgcgaaggg gcggcgatca aggatttcat gcacccggac cgggtggtgc
    22261 tgggcgtggc cacgccacgc gcagaggcac tgctgcgcga gctctatcga ccgctcaacc
    22321 tgatcgaaac gccgatcctg gtgaccgatt gggagagcgc cgagttgatc aaatatgccg
    22381 ccaatgcctt tctggccacc aagatcagct tcatcaacga gatgtcgatg ctgtgcgaga
    22441 gtgtcggggc cgacgtccat gcggtggcga aggggatggg gctcgatggc cgcatcggcc
    22501 gcaaattcct gcatccgggg ccgggctacg gcggctcctg ttttcccaag gacacgctgg
    22561 cgctgattcg catcgcccag gagcatgaca ccccctgccg catcgtcgag agcgtgatcg
    22621 aggtgaacgc ggcgcagaag gcgcgcatgg tgaagaagat tcgcgatgcg ctgggcgggg
    22681 agctgtcggg ccggcagatt gccgtgctgg ggctcacctt caagcccgaa accgacgaca
    22741 tgcgtgatgc gccggcactg tcgattctgc cggcactgct cgacaagggc gcagtggtgc
    22801 gggcgcatga tccgcagggg atggaggagg cgaagaaata tctgcccgac acggtgcgtt
    22861 actacgacga catctatgag atgagcgaag gggtcgatgc cgtggtgctg ctgaccgagt
    22921 ggaacgagta tcgcgggatc gatctggagc ggctgctgca gcggatgcgc ggacgggtct
    22981 ttctcgacct gcgcaatgtc tatgaaccaa cagcgatgcg ggcactcgga ttcgactaca
    23041 ccggcgtcgg ccgttagaaa ctcccgccaa ggcgccggtt gcagtgcgca accggcgctt
    23101 tttcgttggg gttactccag aatctcctgc tgcttcaacg tttcgagcag ctcgccgagc
    23161 gaaccttcgc tcaacagcag atcggccagt ggcgcggctt ctgtcagcgg cgtgatcagc
    23221 aggtggccgg ccgccttgcc ccacgccttg agcgcggcca gatggccggt ggcgccgggg
    23281 aagttgctct gatcgatcag cagcggattg tggcccagct cgcacagtcg ccgttcaagc
    23341 tgttccgcca gctgctgggc gttggcggca gtcagtgcaa ccagtgcggt gcgttgccgg
    23401 aaacggttct cgcgctcgct gcggccgagc cggggcagct ccgcgtcact gtcgctgtcg
    23461 cgccgggcgc cgacgatcat gccggcaccg acggtgatgt tggtcagccg gtcgatgaag
    23521 atgaaggcgc cggtaccgcg gttgttgtcg taggcgtcga acaccagcgg cgcattgaca
    23581 ctgaggtcgc acagcgcgat ctcgttgagc gacagcgtgt ctccgcccgg cagctgttcg
    23641 aggctgttga cgtcgatgcg gtgatggatc tgcggcacct ggccgatcac cgtcttggtg
    23701 ctctgcttga tgtagtactc gcggccgacc ttcagcccat cttcatgcat ccacaccaga
    23761 tgggcgctca ggcgctggct gacctggggc agctgctctg gcctcaccag catgtcgccg
    23821 cggctgatgt cgatctcatc ctccagcgtc agggtgaccg ccatcggtgt ccaggccgtg
    23881 ccgagttcgc catcgtaggt gacgatgctg gcgacccggc tgctcttgcc ggagggcagg
    23941 gcgatgaggc ggtcgccgcg gttgatcacg ccgctggcga tggtgccgga aaagccgcgg
    24001 aagtcgaggt tggggcggtt gacatactgc accggcaggc ggaactggtc gaagttgcgg
    24061 tcttcgctga tctcgatctg ctccagcaac tcgagcagcg gcttgccgtg ataccagggg
    24121 gtctcggtgg agcggtgcac cacgttgtcg ccgttgaggg ccgagatcgg gaggaagtgg
    24181 atgtcgggca ggttgagccg cccggcaaag tcgaggtagt cctgcctgat ctgctcgaac
    24241 cggcccgggt cgaagccgac caggtccatc ttgttgatgg ccaccaccac atggcggatg
    24301 cccagcagcg agacgatgaa gctgtgacgg cgggtctggg tcagcacccc ctggcgggcg
    24361 tcgatcagaa tgatggccag gtcacaggtc gaggcgccgg tggccatgtt gcgggtgtac
    24421 tgctcgtgcc ctggggtgtc ggcgatgatg aacttgcgcc gcgcggtcga gaagtagcgg
    24481 taggcgacat cgatggtgat gccctgctcc cgctcggcct gcagaccatc gaccagcagg
    24541 gccagatcga tgtcgccacc ggtggtgccc gatttgacgc tgtcgctctt gatggcggcc
    24601 agttgatcct cgtagatcat gtgcgagtca tgcagcagac ggccgatcag cgtgctcttg
    24661 ccgtcgtcga cactgccgca ggtgagcagc cgcagcagtt ctttctgctc atgctgctgc
    24721 aggtaggctt cgatgtcggt ggcgatcagg tcggattgat gagacatggt gcaacggatt
    24781 ccttgagttc ggtacggatg gcgggcagcc gggttcagaa gtagccttca cgctttttct
    24841 cttccatcga gccggcctgg tcgaaatcga tcacgcgccc ctgtcgctcc gagctcttgg
    24901 tcagcagcat ctcctggatg atctcggtca ggctggcggc ggtcgattcg atggcgccgg
    24961 tcagtggata gcagccgagg gtgcggaagc gcacctgctt catctgcggt gtttcaccct
    25021 cggccagcgg catccggtcg tcatccacca tgatgtcgac gccgtcgcgg gtgaccaccg
    25081 ggcgctcctt ggcgaagtag agcggcacga tcgggatgct ctccagatag atgtattgcc
    25141 agatgtccag ttcggtccag ttggagagcg ggaagacgcg gatgctctcg cccttgtcga
    25201 tgcggccgtt gtagaggttc cacagctcgg gccgctggtt tttcgggtcc cagcggtggt
    25261 tcttgtcgcg gaaggagtag acccgctcct tggcgcgcga cttctcttcg tcgcggcggg
    25321 ccccgccaaa ggcggcatcg aagccatatt tgtcgagcgc ctgcttgagc ccctgggtct
    25381 tcatcacatc ggtgtgcttc ttgctgccgt ggacgaaggg gctgatgttc atcttcagcc
    25441 cttccgggtt gatgtggacc agcagctcca gccccagctc gcgcgcccgc tggtcgcgga
    25501 actcgatcat ctcgtgaaat ttccaggtgg tgtcgacatg cagcagcggg aagggcggtt
    25561 tgcccggata aaacgccttc atcgccagat gcagcatcac ggcggaatcc ttgccgatcg
    25621 aatagagcat caccggcttg cggaactcgg ccgcgacctc gcgcaggata tggatgcttt
    25681 cggcttcgag cagtttgaga tgggtcagtc tgtggtctgg gatcatggtg acttccgttg
    25741 cgggcgtgaa aggatcatca actcttctca ttcaacatca aatctgcata gtgagttcgg
    25801 gcaaagtctg atcctgaaaa attacaactc ttcgacctgg aggcgcagcg cggcggcctg
    25861 atcgcggcgg ctgctgtcgg tcaggatggc ggtgctggcc agttccggct tcaggtagcg
    25921 ttcggtgacc cgctgcagat cggtcaaggt gacgcgcaga atctgctgcc tgacctgctg
    25981 caggtggtcg gcatcgcggc catgcagctg gttgaagaag gcgcgcttgg cctcgccggc
    26041 aggtgagccg ggcttgtcga tgctgccgat ggcgccgagg atcgcctcgt cgacctgccg
    26101 cagctcatgc tggctgccga gcacccagtc gatggcgcgg tcgaaatcgg ccagcgtctc
    26161 gctcagtcgc ggatcgcggt aggagtagaa gcggaatgcg gcgatgccgg aatcctggcc
    26221 ggcaccaccg ccataggcgc cgccctgttc gcggatggcg cggtgcaggt agccgttgcg
    26281 caggaagccg ccgagcacgg tgagggccgc ggcatcggca tggctggccg gcacggtcgg
    26341 gtaggcgcgg gcgcagaagt tgacctgggt gttggtgagc cacagggcat gggtcggctc
    26401 gcgcagcggc ggcagggcaa agggggtgaa ctcgccctga gcagcggggg gttgcatgtg
    26461 cgccagccgt gccagcagct cggcggtctg ctcgggttcg gcgatcagca gctcctgttt
    26521 gggtgccgcg accagccgtt gatggatctg ctgcagccgt tcggccagcc cggccagtcg
    26581 ttgcggatcg tcgagcgtgc ggtcgagtgc cttgagcgca cggatgccgg ccagtccgct
    26641 ctgccggtgg ccgagacggg ccagtggact catgccggcg gtggcggcgc tcatcgccag
    26701 gctgtggcca ctgccggtga tgctgcgctc gcggctggtg cggatctgcg cgatcagctc
    26761 gcgcagccgc ggcagctcgt cgaagcgcgc ctcggccagc gtgcggtaga gcagctcgac
    26821 gaagggggca tggttgcgca gcagcgcctt gccggagagc accagataac cgcgcagctg
    26881 ctgctcatcc tcggccttgc cgcgcaggga gatctgcgcc gagtagccgc cgctgatctg
    26941 gctttgcagt tgctggatgc tgaggtagtc atccgctccg agccccagat cggtcaggaa
    27001 gctggcatag aggaacagct ccagaagttg cgattcgtcg aactgcggca gatcgatcac
    27061 cagttgctga tagagcagcc cgttggtgcc ctggccatag ctggtgaccg tgcggccggc
    27121 gagctgatcg cggcgctgcg gttcggccag ttgcagggtg gcgggcacat cggccagcgt
    27181 cacccggggc aggatcgact catcgtcgac gcggttctgc cgtgccttca atgcggcggc
    27241 ctgctcgacg atctgccggc tctgctcggc atcgagtccg gccttgatgc gggcgagctg
    27301 tgcggccact gcctgttcgc ggcgctgcgc cagttcgggg tcgggcgcca aggtcaggtg
    27361 gacacggtgg aagttgtcga gcagcagacg gcgcaccagt tgcttgatgt agtcgggatc
    27421 ctcgacatgg cggcgcagct tggccagcgc cggatcgagg tcgatcaact gggcggggtc
    27481 gccgccatgc accgcggcgc ccagcccggc cagcagcagt tgcaggccga agggatagcc
    27541 atcgccgctg atctcgcgct gggtcagctc cagctgatgc agcacggcct cgatctgcgg
    27601 ctgcggcacc ccctcgtcgg cgatcttttc gagcagtgcc atgatgtcgg cggccaactg
    27661 ttcggcatgt tcggggcgac tgccttcgag gccgcagacg aacagcagtt cgcggttgcc
    27721 atcttccagt ccgcacagcg gtgacggggc gctgccgagc gcagaggttt cgagcagttg
    27781 cagcagcggc gacgcactgt tgtcgagcag caccgcgctc agaaaacgcg cttcgagcag
    27841 ctcgtcgagg tcggtgcagt ggccgagcag ccagccgatc accagatggg tgcgatcgac
    27901 ttcgtccgct tcggcttcgg tcgggtagta ctccagcacc ttcaccggcg catgaaagcg
    27961 ctgctcgtca tgcagcggca gcatgatctc gcggcggtcg aagcggtgca gcgcctgctg
    28021 ctcgaaacgc tgttgcaact cggccactgg caggttgccg aaggtcatga agatggcgtt
    28081 gctcgggtgg tagtggtcgc ggtagaagtc gagcagttgc gcatggctca gctgcgggat
    28141 gcagtcgggc tcgccgccgc tgttgaagtg gtaggtggtg gtcgggtaga gatgcctggt
    28201 cagggtctgc cacagcaggc tggtgggcga gctcatcgcg cccttcatct cgttgtagac
    28261 cacgccacgg tagcagagcg gcgtcgaagg gtcgtccggc tgttcgaatt cgagccggtg
    28321 gccctcctgg gcgaagtcga gcgggtcgag ccgggagaag aaggtggcgt cgaggtagac
    28381 atcgagcagg ttgaagaaat ccttgcggtt ctggctggcg aacgggtagg cggtccagtc
    28441 gctgctggtg aaggcattca tgaaggtgtt gagcgagcgg cgaatcatca tgaagaacgg
    28501 gtcgcgcacc ggatagcgtt cgctgccgca cagcgaggtg tgttcgagaa tgtgggccac
    28561 gccggtcgag tccatcggca ccgtcgggaa ggcgaccaga aagacatttt cgtcatggtc
    28621 ggcgctgatg tggaagtgac gggcgccggt gcggccgtgg cggtactcat tgagcagcag
    28681 cttgagtgcc ggaatcggat gctgccgcac gagttcgaag ctgggatggg gcagggtgcc
    28741 ggacgccgtc gttgaagggt tgggcatggt ctggatcttg tcctgagtga aagggtgcaa
    28801 tgctgccatc aagtgtaatc aaccgccatg ccactgtcag gcggtggtgt cagtcgcatt
    28861 ggcgcggatc gctgcctggt ggttgagccg cacgtctggc ccgggcgcgc atcaccgccg
    28921 ccaggatgtc cgcctgtgtg ggtgagctgt gcgcagtggc ggcgactgct ggattgcgtg
    28981 tctgctgacg gcgctcggcc tgttcggccg cgaggcgcgc caaccgtgcc gtgcgcgcct
    29041 gatggcggcg gcggaactgg tcggcctgtg cgcgccgttg tggccatgag cgctgcgttt
    29101 catcctcgat tcgaaccaga tcgatgcagt cgaccgggca gggttcgatg cagagctcac
    29161 agccgctgca ctcgctggcg atgacgctgt gcatccattg cgcggcgccg acgatcgcat
    29221 cgaccggaca ggcctgaatg cacttggtgc agcc
//
LOCUS       DAORCQ010000023        28686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_891_length_28686_cov_6.732423, whole genome shotgun sequence.
ACCESSION   DAORCQ010000023 DAORCQ010000000
VERSION     DAORCQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 28686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28686
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_28686_cov_6.732423"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKI23_03930"
     CDS             <1..688
                     /locus_tag="PKI23_03930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNN36124.1"
                     /translation="DRGAKQPVRTIPTVQSPTQTRVYLIDKPGSIQSTIFAGDLLTPG
                     GGDDEIAVDLMNQILGGTFTSRLNMNLREDKHWSYGARSLVVDASGQRPFLAYSSVQT
                     DQTAPAMAEILNEVRVLTGQNPPNGAEINKVKRFETLKLPGKYETGAALGGAIADLVR
                     FKRAESYITDYPGRVSDTSVEAIVTAARQLHPDQLTWVVVGDRARIEKAIRELKIGEI
                     ELLSTDGSAQ"
     gene            685..1185
                     /locus_tag="PKI23_03935"
     CDS             685..1185
                     /locus_tag="PKI23_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNN36125.1"
                     /translation="MKWPRAAVIAALLLAGCASTPPQIANPPAQPLSVSEATGHLAEH
                     LHQPVRWGGIILSVTPQPEEIWIEIEARPLGHWGHPNEDAASLGRFYARLPATRMDSD
                     LRSGRLITLYGVLSDEATQQLGNRRILLPVMTAEQFSTWERRAEQPPAFPRSPYGWPY
                     PPYYFP"
     gene            1222..2331
                     /locus_tag="PKI23_03940"
     CDS             1222..2331
                     /locus_tag="PKI23_03940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN36126.1"
                     /translation="MSTAAPIAFFFSTSGHSGVDRCVRNLLPAITGRGHRIDLLHVRG
                     HGPSIENLPAGVRVVDLGTRHTYSALPAVVRYLRQNRPVAMLSDKDRVNRTALLARWL
                     AGVPTRLVLRSGTTISIDLAHRGAFERWLQRNSMGKLYRFADQVIVPAQDVADDLSRY
                     TGLAREHIQVVPSPVVPASCFERNASRPDHPWLQPGEPPVVLGVGELCMRKDFAMLLR
                     AFAQVRAQRDCRLLILGKGRQHDQLLALAASLGVAQDVCLAGFQPNPYDYMQHAALLA
                     FSSRWEGLGFVLIEALALGTPVVSTDCPSGPREILHDGRYGPLVPVGDDQAMARAILD
                     TLEQPLPPATLQQAARRYEIEAATDAYLTALGLPP"
     gene            2328..3242
                     /locus_tag="PKI23_03945"
     CDS             2328..3242
                     /locus_tag="PKI23_03945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNN36127.1"
                     /translation="MNDRAARRLADRVTAIVVTYNSAAVLSAALDSIAELDHCLVVDN
                     ASHDQSCTLVESGHPRVRLLKNPRNLGFGVANNRAAALADTEFLLLLNPDARLLPGAL
                     AALVDAADRYPDAAILAPRLVDETGQPRAILRHPFFRRHKIKGRFDQSWIAGDLCAEM
                     LSGAVMLIRRAWFAGEPLFDPALFLYYEDDDLCLRARARQGALVIVAGALAYHAEDCS
                     SLPCARLSALKQRETIASQLHLIRKYRGGSASLAQAALLLLRNLLNIPFYLLTGKARR
                     ARRSLHKVAGTLLFMQRWASGYPSSSRR"
     gene            complement(3218..4348)
                     /locus_tag="PKI23_03950"
     CDS             complement(3218..4348)
                     /locus_tag="PKI23_03950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN36128.1"
                     /translation="MAELSAEAGVQRPLCLFIYQNRDGGVERNMANLAQGFADRGIAV
                     DLLVSDATGPFMQPLAAGVRVIGLGKGSKRTVIAALARYLRQNRPRAVITGKPSDDRF
                     ILRARRRAGVESRCFFGAGTAISRQIEERGFHPLRRRWKAWRRGRLYRQADGIIATSN
                     WVARDIARLSGLPVERITVIGNPVVTPQLHPLAAEPVEHPFFCPGAVPVIIAAGRLSH
                     EKDFSTLLRAFGLLRRSHDARLLILGEGAQRPMLEQLVAQLGLDGAVSLPGFVANPYA
                     WMARARLFVLSSLWEGFGNVLVEAMAVGTPVVSTDCPGGPREILHDGAYGPLVPVGDA
                     VALSRAMARMLDQPTAAGLLREAAQSYSVEACVARYLRLLEG"
     gene            complement(4341..5003)
                     /gene="yihA"
                     /locus_tag="PKI23_03955"
     CDS             complement(4341..5003)
                     /gene="yihA"
                     /locus_tag="PKI23_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNN36129.1"
                     /translation="MTQPPPAVNPFRRAVFMKSALLLNDCPADGGREVAFCGRSNAGK
                     SSAINTLTESARLARTSKTPGRTQLLNFFALNESARLVDLPGYGYAQTSKEIQAHWKR
                     HLDHYLRERLSLVGLVLVMDIRHPLRPFDWMMLEWAQARVLPVCILLTKADKLSRNQA
                     QQSRLKVLKSLAEVNISEQVNVQCFSTLSREGMAELIALLKGWLALDGPPSDGEESGP
                     HG"
     gene            5167..5772
                     /locus_tag="PKI23_03960"
     CDS             5167..5772
                     /locus_tag="PKI23_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNN36130.1"
                     /translation="MQRLFLTAVLTIGLIGSVQAAGDATAGQAKAAACAACHGADGNS
                     AAPNFPKLAGQGERYLLKQMHEIKSGARAAPLMAGQLDNLSEQEMADIAAYFAAQKGT
                     VGAAKADLVKQGEELYRAGSKRKNAAACSACHSPTGQGNDLAGFPSLSGQHAQYTAAQ
                     LQAFRNGTRSNDAERIMRDNAELLSDAEIEAVASYISGLHP"
     gene            5869..6537
                     /locus_tag="PKI23_03965"
     CDS             5869..6537
                     /locus_tag="PKI23_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNN36131.1"
                     /translation="MNTVIRHHWRWILSALLLGLLPLQLRAESGYQEGVHYQKLESAV
                     ITRDPGKIEVIEVFWYGCPHCYKLEPTLQHWSANLSRDVDFWRSPAMWNGRMELHAQA
                     FYTAEALNLLEKLHKPLFDALNPQGQQLASKEEIRTLFTRQGVKAEEFDQVFDSFGVQ
                     SKVKQANSRFRALRLTGVPAFIVNGKYRVTGEKLKSEDEMLKVVDYLIGQERKARAAP
                     AAVR"
     gene            6551..7387
                     /locus_tag="PKI23_03970"
     CDS             6551..7387
                     /locus_tag="PKI23_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008092073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNN36132.1"
                     /translation="MHEHQAHPPSPQAAEALPTAGQKLRLLTYNMQVGIATHRYRHYI
                     TRGWQHLLPHGLRERNLERIAELIQTFDIVALQEADGGSLRSGFVNQVEYLAHRARFP
                     YWYQQRNRNLGRLGQHGNGLLTRFPPEILEDHKLPGALPGRGAIVARFAGPVEPLLVV
                     GVHLSLGARSQRLQLSYLLELMSNYRHVIVMGDMNSETERLLLESPLQHGNFHRPAAE
                     LATYPSWRPERAIDHILISRSITIDRTEVLDFPFSDHLPVAVELTLPSAVRLPVTATG
                     LH"
     gene            7423..8358
                     /locus_tag="PKI23_03975"
     CDS             7423..8358
                     /locus_tag="PKI23_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNN36133.1"
                     /translation="MTLNELRYLVALARERHFGRAADTCFVTQPTLSVAIKKIEGELG
                     ITLFERNASQVTLTEAGIPIVTQAQRVLDELALLRSLAQEMRNPLELPVRIGAIHTIG
                     PYLFPALIPRLREQAPKLPLQITEGTTDVLLSQLRLGELDLLVLALPFAQSGLTVRPL
                     YDEPLLLALPHDHPWNQRDTIDIDALGHERMLLLGEGHCLRRQVLELCPNCLLRSEER
                     RAQPTLIGNSLETLCNMVAAGNGVTVLPATAVHRIAGEERGLMTVRPFTAPAPQRRVV
                     LAWRNSFSRNALTDLVAEAIQQVSAPLGARITADG"
     gene            complement(8382..9518)
                     /locus_tag="PKI23_03980"
     CDS             complement(8382..9518)
                     /locus_tag="PKI23_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNN36134.1"
                     /translation="MNFEDFLRMMVEQGASDLFITAGIPPSMKVNGRVVPIGRTPLGP
                     EQSRELVFGVMNDQQRLDFIRNHECNFAISARGLARFRVSAFHQRNLAGMVLRRIETR
                     IPEIGELGLPDILKDLSMTKRGLVVFVGATGTGKSTSLASMIGYRNQNSHGHIISIED
                     PIEFLHQHRNCIVTQREVGIDTDSFEVALKNTLRQAPDVIMIGEVRSREVMGHAIAFA
                     ETGHLCLCTLHANNANQALERIIHFFPADRHNQIWMDLSLNLKAIVAQQLIPTADGNG
                     RRAAIEVMLNTPLVSDLIRKGEVHNIKPLMTRSRELGMQTFDQALYDLFSSGIITFEN
                     AIAHADSPNDLRLMIKLGKSGVSQEIGKSAANLTLADDYEPPRR"
     gene            complement(9573..10607)
                     /locus_tag="PKI23_03985"
     CDS             complement(9573..10607)
                     /locus_tag="PKI23_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNN36135.1"
                     /translation="MDITEMLAFCVKQKASDLHLSAGLPPIIRVDGDVRRINLPPLEH
                     KEVHELIYEIMNDKQRRDFEEFLETDFSFEVPGLARFRVNAFNQNRGAGAVFRTIPSK
                     VLTMEELGHGQVFREVSMVERGLILVTGPTGSGKSTTLAAMVDYINENRYSHILTIED
                     PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGELRDLETIRLALTAA
                     ETGHVVFGTLHTNSAAKTIDRIVDVFPGEEKAMVRSMLSESLQAVISQTLLKRVGGGR
                     VAAHEIMLATPAIRNLIREDKIAQMYSAIQTGGGMGMQTMDQSLRNLLQKGLITRQAA
                     REKAKQPDAF"
     gene            10732..11439
                     /locus_tag="PKI23_03990"
     CDS             10732..11439
                     /locus_tag="PKI23_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015649764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNN36136.1"
                     /translation="MISIAENLAALTRRIHDAEQHHGRRPGSVTLLAVSKGQPAAQIR
                     AAHQAGVRHVGESYLQEALPKQAELADLDLCWHFIGSLQANKSRAVASHFDWLHSLDR
                     LQLAQRLDAQRPAELPPLNVLIQVGFEQRTGRAGVAPDDLPRLAGQIAQLPRLRLRGL
                     MTLPLPQQACSPHPFVRLADLLDRLNGAGHRLDTLSMGMSDDFEAAIAAGATLIRIGS
                     ALFGQRPTPFTAQTQYG"
     gene            11450..12289
                     /gene="proC"
                     /locus_tag="PKI23_03995"
     CDS             11450..12289
                     /gene="proC"
                     /locus_tag="PKI23_03995"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNN36137.1"
                     /translation="MHPATARLAFIGAGNMSSSLIGGLLEQGYPASQIIATDPSPQQR
                     DAVANRFGIEVSADNVAAVATAEVVLLAVKPQVLKAVALELAPALGHRPLLLSIAAGI
                     GLESLASWLGPQLPLVRCMPNTPALVRSGAAALCGNRQVSAAQRELSLGLLEAMGTAL
                     WLEDESLLDAVTAISGSGPAYFFLLIEALQMAAVELGLPAETARQLTLQTALGAARMA
                     LAEPISAAELRRRVTSPGGTTERAIQVFEAGGFTALVRQAALAAAQRSAELATELGEP
                     ATR"
     gene            12331..12921
                     /locus_tag="PKI23_04000"
     CDS             12331..12921
                     /locus_tag="PKI23_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNN36138.1"
                     /translation="MGQLSHAGIYLVQTLVSLYLLAVLLRFFLQLVRADFYNPVSQFV
                     VKVTDPLLRPLRRLIPGFGGVDLAALVLALLVQGVAIALILSLAGAPLPNPLLILLWS
                     LVGLVGLTIKIFFVALLLTIILSWVAPMSHHPLADLLLQLTEPVMAPVRRIIPPIGGL
                     DLSPIFVFIGINLLEMLVVDSLVTLLAMPRGLVLGL"
     gene            12918..13211
                     /locus_tag="PKI23_04005"
     CDS             12918..13211
                     /locus_tag="PKI23_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HNN36139.1"
                     /translation="MSAHYRWEGDRLLLSVRLTPRAGHSRIIGPVEGALRVGIAAAPV
                     DGAANAELLRLLASCFAVPKSRVALLRGDRSRSKQLAIERPQQLPAEAEIKVQ"
     gene            13305..14465
                     /locus_tag="PKI23_04010"
     CDS             13305..14465
                     /locus_tag="PKI23_04010"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNN36140.1"
                     /translation="MPDSIPPDSVGIVTPQRQRFDLPLPLDCGRSLPGFELVYETYGT
                     LNAQRDNAVLICHALSGNHHAAGYHGMEERKPGWWDVCIGPGKPIDTNRFFVLALNNL
                     GGCDGSTGPSSLNPATGRSYGPDFPMVTTADWVRSQACLADHLGIDRFAAVVGGSLGG
                     MQALQWSVDLPQRLRHAVVIAAAPKLTAQNIAFNEVARQAIMKDPDFHGGRYLEHGTY
                     PKQGLRLARMVGHITYLSDDAMGQKFGRELHSDTLRYDFGTQFQIESYLNYQGEIFSE
                     RFDANTYLHMTRALDYFDLAKAHGDDLVAAFRQARCSFLVVSFTTDWRFSSARSRELV
                     TALVEAERNVSYADIEAAQGHDAFLLPIPRYMELLRAYFDRIANESWPVAAA"
     gene            14462..15052
                     /gene="metW"
                     /locus_tag="PKI23_04015"
     CDS             14462..15052
                     /gene="metW"
                     /locus_tag="PKI23_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNN36141.1"
                     /translation="MRPDLGIIMEWIAAGSRVLDLGCGDGTLLAELTRQKGVHGWGIE
                     NNPHDISHCIAQGVNVIEHDLDQGLAEFRTQRFDTVVMSLALQAVRFPDLILDEMLQI
                     GRECIVTFPNFGHWSCRHDIGLRGRMPRSDFLPHEWYNTPNIHFCTIADFEALCRKKN
                     FQILNQRVVDRHYRSNPLMRRWPNLLAEIAIYRLTR"
     gene            15063..15503
                     /locus_tag="PKI23_04020"
     CDS             15063..15503
                     /locus_tag="PKI23_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HNN36142.1"
                     /translation="MHHRILLLLSLMLLGVQLAQAEQMVRFDGYELHYNVSPSRLLTT
                     PTAARLGIVQAPNRALVNVTVLKLQADGRSQPVTSQVQGQARNLMGQSVPLQFRTVIE
                     SDQPSAIAELRFDDEELFHFELEVTPAGSDRGYPLRFDQQLFIE"
     gene            15500..16138
                     /gene="rdgB"
                     /locus_tag="PKI23_04025"
     CDS             15500..16138
                     /gene="rdgB"
                     /locus_tag="PKI23_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNN36143.1"
                     /translation="MINEDSGQRMDHSATFRQRMVLASNNAGKLREFADLFAGLRIEL
                     LPQSAFAVAEVEESGLTFVENALLKARAASVASGLPAVADDSGLAVDALGGAPGLYSA
                     RYAGAGAGDAANNARLLAALAGVPEAQRTARYHCLLVLLRHAEDPAPLISHGIWEGRI
                     LTAARGAGGFGYDPLFWVESEQASAAELAPARKNQLSHRGQALRHLLRQLQP"
     gene            16135..17301
                     /gene="hemW"
                     /locus_tag="PKI23_04030"
     CDS             16135..17301
                     /gene="hemW"
                     /locus_tag="PKI23_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNN36144.1"
                     /translation="MKRETTPRRFGRLPPLGLYVHIPWCVRKCPYCDFNSHAADGSLP
                     EADYVEALLDDLLQDLPLAQDRPLQSLFIGGGTPSLFSADAIARLLEGIGALLPFTRD
                     IEITLEANPGAADQARFRGYRAAGVNRLSLGIQSFDNDKLRALGRIHDAEAADRAIEA
                     ARAAGFDNFNLDLMFGLPQQSIEAALADLHRAIDHRPTHLSWYQLTIEPNTAFHKRPP
                     PLPDDELLWPMQQQGQRLLAAAGFEQYEISAYCQPQRHSKHNLNYWKFGDYLGIGAGA
                     HGKITLGSAQQVIRTRKSRQPRHYLDHQKSYLAGKQVLNEADLPLEFLMNGLRLNSGV
                     SSQSFVQRTGLPLSSIDDALLQAREKGLLEEESTRLNATLRGRQYLNSLLELFL"
     gene            complement(17321..18034)
                     /gene="trmB"
                     /locus_tag="PKI23_04035"
     CDS             complement(17321..18034)
                     /gene="trmB"
                     /locus_tag="PKI23_04035"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNN36145.1"
                     /translation="MSVPASDRDLRAIRSFVIRGGRLSPAQQRALEQLWAHYGIDWQP
                     DQPLDWTTCFGRTAPVVLEIGFGMGESLVTMAQNAPECDFVGVEVHPPGIGNLLQRIE
                     QERLGNLRLLRGDVMAMLHAGVMPEGSLTRVQIYFPDPWPKKRHHKRRLVQAALLDLL
                     HRVVRPQGQLHIATDWQPYAEAMLLSLEQASGWRNLAADGGFVPRPPWRPVTKFERRG
                     ERLGHGVWDLLFERSDRDG"
     gene            complement(18055..18861)
                     /locus_tag="PKI23_04040"
     CDS             complement(18055..18861)
                     /locus_tag="PKI23_04040"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNN36146.1"
                     /translation="MDSTQPIQPQQDPLLIGGVSYHSRLLVGTGKYRDFDETRRAVEA
                     SGAQIVTFAVRRVNIGQQADAPSLLDILPPTRYTLLPNTAGCYSARDAVRTCQLAREL
                     LDGAALVKLEVLGDEKTLYPNVTETLIAAEALVKEGFAVMVYTNDDPLIAQRLEQLGC
                     IAVMPLGAPIGSGLGIRNPYNIRTIVENATVPILVDAGVGTASDAAIAMELGCDGVLM
                     NTAIAEATQPVWMAAAMKKAVEAGREAYLAGRMPRRRYASASSPLDGRFF"
     gene            complement(18881..19096)
                     /gene="thiS"
                     /locus_tag="PKI23_04045"
     CDS             complement(18881..19096)
                     /gene="thiS"
                     /locus_tag="PKI23_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNN36147.1"
                     /translation="MSSAELTIQLNGVPQQVPAGLTIAGLIERLALQQRRIAVELNGT
                     IVPRTLHAQQTVQAGDQIEVVTAIGGG"
     gene            complement(19093..19473)
                     /locus_tag="PKI23_04050"
     CDS             complement(19093..19473)
                     /locus_tag="PKI23_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNN36148.1"
                     /translation="MTARGWLVAGAVSGALAVMTGAFGAHALKARLAAAQLDTWATAV
                     QYHFYHALALIAVGLAMRLLPEVAGLHRVGWLFLFGLLAFSGSLYLLALTGVRQLGFI
                     TPLGGVAFIAGWLLLAHLLWRGLR"
     gene            complement(19549..20397)
                     /gene="rpoH"
                     /locus_tag="PKI23_04055"
     CDS             complement(19549..20397)
                     /gene="rpoH"
                     /locus_tag="PKI23_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNN36149.1"
                     /translation="MSTDILPMNVLAPVGNLNAYLQAVQRIPMLSQEDEQRLARQYSE
                     EGDLDAARQLVLSHLRFVAHVARGYAGYGLPQADLIQEGNIGLMKAVKRFDPNMGVRL
                     VSFAVHWIKAEIHEFILRNWRIAKIATTKAQRKLFFNLRGAKKRLDWLSNDEVSSIAR
                     ELNVDPKTVRQMEGRLAAHDTSFDAQDDDDSSPPAPAEYLEDSSADPALILEQLDHGS
                     HQGEQLLMALEALDPRSRDILERRWLNEPKATLHDLAAIHGVSAERIRQLEQNAMKRL
                     RSAIDA"
     gene            complement(20477..21448)
                     /gene="ftsX"
                     /locus_tag="PKI23_04060"
     CDS             complement(20477..21448)
                     /gene="ftsX"
                     /locus_tag="PKI23_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNN36150.1"
                     /translation="MARGQNSARRQPPGWRDRLRGYVLQHRSHCRDSLRRLLAEPMAT
                     LMIWSMVGIALALPSGLYLLLDNLKQVSEGWSEGSRRITLYLKIEQSDEMARLLGERL
                     GRDAEIAAVKVIGREAALLEFRQRSKLDDVIDALGSNPLPPLLLITPRADSGGIEPLR
                     ALARRLATLPEVEQAQVDTEWLARLLALIELGQRAVLALTLLLAVAVLLIVATTLRLT
                     ITNRRREIEVIKLVGGTAAFIRRPFLYTGLWYGLGGGMAALLLTGALLLWLRAPVQEL
                     AALYQSSFTPVGLGMHGTTILLLGSALLGVAGAWIAAERQLRSIEPD"
     gene            complement(21457..22143)
                     /gene="ftsE"
                     /locus_tag="PKI23_04065"
     CDS             complement(21457..22143)
                     /gene="ftsE"
                     /locus_tag="PKI23_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNN36151.1"
                     /translation="MLRFEQVSKRYEGGHEALRDLSFHLPKRSLAFLTGHSGAGKSTL
                     LKLIMRMERPSQGRVLVGGIDLDRLPQRSIPFYRRNIGVVFQNHRLLFDRTLFDNVAL
                     PLVIAGASRHEIGRRVRAALDKVGLLDKERMNPIQLSGGEQQRAGIARAVVSRPPLLL
                     ADEPTGNLDPDLSAEIMALFSAFSQAGVTVLIASHDTALIDAMDKPVFELHEGRLIND
                     PFDDGDAAPP"
     gene            complement(22147..23301)
                     /gene="ftsY"
                     /locus_tag="PKI23_04070"
     CDS             complement(22147..23301)
                     /gene="ftsY"
                     /locus_tag="PKI23_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNN36152.1"
                     /translation="MFKFLKRNNTPQTPEAAGSGTASPSAEHDAEAPESSPSPIDALL
                     HEPAPTADTEPQQAQAAPTVAAPAETEPSASKPGLFSRIRSGLTRTRVSLAEGLSNLI
                     LGRKQIDAELLEALEEQLLLADVGMAATRQIIDQLSDRVARKQLADADALHAALRAAL
                     SEILQPVSLPLQIDDSKRPYVLLVIGVNGVGKTTTIGKLAKRYGQQGKKVMLAAGDTF
                     RAAAVEQLKVWGERNQVPVVAQQSGADSASVLFDALSAAKARGIDLLIADTAGRLHNK
                     AHLLEELKKVVRVLKKIDPEAPHEVLLVLDASTGQNALNQAQQFRDAVDVSGIALTKL
                     DGTAKGGVIFAIAQQLGLPIRFIGVGEAIDDLRPFDADEFIRALFERDPA"
     gene            23459..24052
                     /gene="rsmD"
                     /locus_tag="PKI23_04075"
     CDS             23459..24052
                     /gene="rsmD"
                     /locus_tag="PKI23_04075"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNN36153.1"
                     /translation="MNPAHTAPGRIRIIAGRWRGRRLPVIASEMLRPTPDRVRETLFN
                     WIGPAVRGTRCLDLFAGTGILGLEALSRGAAQVVWIEADIRLVRALRQIQATLQAGGE
                     VVCGDVTDWLTGFDPARSAPFDLVFLDPPFRRGLLEQVVQQLEQRALLSPQALIYLEH
                     EREHQPALPPNWLPLRATHAGQVTAQLLRRQPVAQSD"
     gene            24116..24601
                     /gene="coaD"
                     /locus_tag="PKI23_04080"
     CDS             24116..24601
                     /gene="coaD"
                     /locus_tag="PKI23_04080"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNN36154.1"
                     /translation="MKTAIYPGTFNPLTNGHVDILERAARLFDRVLLGIAPSAGKQPL
                     FTLQERIDLARLCVAHLPNVEVLAIEGLLINFSRQHDAIAIVRGIRAVSDYEYELQLT
                     NMNRAMAPQIETIFLTPADNLSFISSTLVREIASFHGDVSKFVRPEVATALQAKFPPP
                     K"
     gene            24621..24878
                     /locus_tag="PKI23_04085"
     CDS             24621..24878
                     /locus_tag="PKI23_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNN36155.1"
                     /translation="MALMITSACINCDVCEPECPNGAIHAGELVYEIDPALCTECVGH
                     FDRPQCIEICPVDCILTDPECIESPQALQRKFQQLHPSTEP"
     gene            complement(24888..25238)
                     /locus_tag="PKI23_04090"
     CDS             complement(24888..25238)
                     /locus_tag="PKI23_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HNN36156.1"
                     /translation="MNLRNRRSLCQTGILTLSLLLGTQPLWAGSSDDGNRPGAGAMAA
                     DLLVARPLLIGMTVVGTGLFIVSLPFSALGGNVDEAADQLVAGPARNAFVRCLGCTAA
                     SRKPAPAVGDGPTQ"
     gene            complement(25314..26408)
                     /locus_tag="PKI23_04095"
     CDS             complement(25314..26408)
                     /locus_tag="PKI23_04095"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNN36157.1"
                     /translation="MKLSDRSQSRDNNFNLIRMVAACSVLVSHSFALTGSGEDPVKRL
                     IGMVTLGDLAVDLFFVASGFLVTASLLKRQNGVDFVWARFLRIYPAYWLMLLMMAFVL
                     GPLFTTLPPSDYFAQSSIYVRLAKAATVFIHTASTLPGVFTTNPLPNAVNGSLWTMPY
                     EVRMYAILAVTWLLLRWRSDRIRALQRAVIVYALLAAGYLLAEEFALIDQSPRLHQLA
                     RFIFMFFSGATFYVLRARIRLHGALFWPLLLLLLLSVEHRHLFFVLYHGSIAYLLLYL
                     AYVPSGLVRGYNRVGDYSYGFYIYAFPVQQAVVALIPDVSVLEMVLIAVPVVLLLSAL
                     SWHLLEKRVLTLKERYVQRTRAWLGAAPAE"
     gene            complement(26405..27223)
                     /gene="mutM"
                     /locus_tag="PKI23_04100"
     CDS             complement(26405..27223)
                     /gene="mutM"
                     /locus_tag="PKI23_04100"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNN36158.1"
                     /translation="MPELPEVETTLRGLAPHLIGRRIHDMVLRNGRLRWPIPDELPER
                     LRDRRIGSLQRRAKYLLLQLDGGHLLLHLGMSGRFMLLEPPAVAPTAHDHYDLLLEGG
                     ALLRYRDPRRFGCCLWLEGDPLTHPLLSRLGPEPLGEAFDGGHLHRSARGRTVAIKSL
                     LMQSEVVVGIGNIYANEALHRAGIDPRRPAGTLSHADCERLVGQIRRLLEQAITQGGT
                     TLRDFLNSDGQAGYFGFALDVYGRTGQPCPRCGGQVASIRLNQRSTFFCQECQQ"
     gene            27317..>28686
                     /locus_tag="PKI23_04105"
     CDS             27317..>28686
                     /locus_tag="PKI23_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNN36159.1"
                     /translation="MMVLGIDTGGTFTDFVLFDGERLHIHKVLSTPEAPDEAIATGMA
                     ELGLEAPLRQGKLLLVHGSTVATNAALEGKGVPTLFVTNRGFRDLLTIGRQIRRELYE
                     LMPAPLPPPVPAELCFEVDTRRDHLGRLITPLTDADLTRLRHAIATLAPQAVAICLLY
                     SFMDESEEQALAAALPPGLFVSCSSQVLPEYREYERGIATWLNSWLGPLVSDYLGRLS
                     ARTAPAPLTIMQSSGGTVAAEQARQRAVNLLLSGPAGGMAAARHLADTLGERQLITFD
                     MGGTSTDVGLLSGGELTLTNEGRIGPYPVAVPMVDLHTIGAGGGSIAFLDDGGGLWVG
                     PRSAGALPGPACYGRGGREPTVTDANLLLGRLRPERFLGGAMPLDVAAAQRAIATLAD
                     PLGLSLEETAAGIIEIANEQMIRALRLISVERGHDPRQFRLCCFGGAGGLHLCRLAEA
                     LGIRRAL"
BASE COUNT         4953 a         9780 c         9151 g         4802 t
ORIGIN      
        1 cgaccgtggc gcaaaacagc ccgtccgcac cattcccacc gtacagtcgc cgacgcagac
       61 ccgggtctat ctgatcgaca agcccggttc gatccagtcg accatcttcg ccggtgacct
      121 gctcaccccc ggtggcggtg acgatgaaat cgccgtcgac ctgatgaacc agattctggg
      181 cggcaccttc acctcgcggc tcaacatgaa tctgcgcgaa gacaagcact ggtcctatgg
      241 tgctcgcagc ctggtcgtcg atgccagcgg ccagcgcccg tttctcgcct acagctcggt
      301 gcagaccgat cagaccgctc cggccatggc cgagatcctg aacgaggtcc gggtgctgac
      361 cggacagaac ccgcccaatg gcgccgagat caacaaggtc aagcggttcg agacgctgaa
      421 attgcccgga aagtatgaaa ccggcgcggc cctgggtggc gccatcgccg atctggtgcg
      481 gttcaagcgg gccgagagtt acatcaccga ctaccctggg cgggtcagcg acacctcggt
      541 cgaggcgatc gtcaccgccg ccagacagct gcatcccgac cagctcacct gggtggtggt
      601 gggcgatcgg gccaggatcg aaaaggcgat ccgcgagctc aagatcggcg agatcgagct
      661 gctctcgacc gacggttcag cgcagtgaaa tggccgcgcg cggcggtgat cgcggcactg
      721 ctgctggccg gttgtgcctc gaccccgcca cagatcgcca acccgccggc gcaaccgctg
      781 tcggtgagtg aagccaccgg gcatctggcc gagcacctgc atcaaccggt gcgctggggc
      841 ggcatcattc tgtcggtcac cccgcaaccc gaagagatct ggatcgagat cgaggcgcga
      901 cccttgggcc actggggcca cccgaacgaa gatgccgctt cgctgggtcg tttctatgcc
      961 cgactgccag ccacccgcat ggattccgat ctgcgcagcg ggcggctcat cacgctctat
     1021 ggcgtgctga gcgatgaggc gacccagcag ctgggcaacc gccgtatcct gctgccggtg
     1081 atgaccgccg agcagttcag cacctgggag cgccgcgccg aacagccacc ggccttcccg
     1141 cgttctccct atggttggcc ctacccgcct tactactttc cctgaaccgc gctttctctc
     1201 caaagcccac cgccagcacc gatgagcaca gccgcaccca tcgccttctt cttctccacc
     1261 tccggacaca gcggtgtcga ccgctgcgtg cgcaatctgc tgccggcgat caccgggcgc
     1321 ggccatcgga tcgatctgct gcatgtgcgc gggcatggac cgagcatcga gaacctgccg
     1381 gccggcgtgc gggtggtcga tctgggcacc cgccacacct actcggcgct gccggccgtg
     1441 gtccgttatc tgcgccagaa ccgcccggtg gcgatgctga gcgacaagga ccgggtcaac
     1501 cgcaccgcac tgctggcgcg ctggctggcc ggcgtgccca cccggctggt actgcgctcc
     1561 ggcaccacca tctcgatcga cctggcccac cgcggtgcct tcgaacgctg gctgcagcgc
     1621 aactcgatgg gcaagctcta ccgctttgcc gatcaggtga tcgtgccagc ccaggatgtc
     1681 gccgatgacc tgagccgcta caccggcctg gcccgcgagc acatccaggt ggtgccaagc
     1741 ccggtggtgc cggcatcctg tttcgaacgc aatgcatcac gccccgatca cccctggctg
     1801 caaccgggcg aaccaccggt ggttctcggt gtcggcgagc tgtgcatgcg caaggatttc
     1861 gccatgctgc tgcgcgcctt cgcccaggtg cgggcgcagc gcgactgccg cctgctgatt
     1921 ctcggcaagg gccgccagca tgaccaactg ctggcgctgg ctgcctcact gggggtggcg
     1981 caggatgtct gcctggccgg cttccagccc aacccttacg actacatgca acacgccgcg
     2041 ctgctggcct tcagctcccg ctgggaaggg ctcggctttg tactgatcga agccttggcg
     2101 ctgggcacac cggtggtctc gaccgactgc ccgagtggcc cgcgcgagat tctgcacgac
     2161 gggcggtacg gcccgctggt gccggtgggc gatgaccagg ccatggcgcg cgccatcctc
     2221 gacacgctgg agcagccgct gccgcccgcc accttgcaac aggcggcacg ccgttatgag
     2281 atcgaggccg ccaccgacgc ctatctgacc gcgctgggcc tgccaccatg aacgacaggg
     2341 cggctcgccg cctcgccgac cgggtcaccg caatcgtcgt cacctacaac agcgctgccg
     2401 tgctcagcgc cgcgctcgat tcgattgccg aactcgacca ctgtctggtc gtcgacaatg
     2461 ccagccatga tcagagctgc acgctggtcg agagcggcca tccgcgggtc aggctactga
     2521 aaaacccgcg caatctgggg tttggcgtgg ccaacaaccg tgccgccgca ctggccgaca
     2581 ccgaattcct gctgctgctg aaccccgatg cacggctgct gcctggcgca ctggctgcgc
     2641 tggtcgatgc agccgaccgc tatcccgacg ccgccattct ggcacctcgg ctggtcgatg
     2701 aaaccggtca gccgcgcgcc atcctgcgcc accccttctt caggcgccac aagatcaagg
     2761 gccggttcga ccaatcctgg attgccggcg atctgtgcgc cgagatgctc tccggcgcgg
     2821 tgatgctgat ccggcgcgcg tggttcgccg gcgaaccgct gttcgacccg gcactgttcc
     2881 tctattacga agatgacgac ctctgtctgc gcgcccgcgc ccggcagggt gcgctggtca
     2941 tcgtcgccgg cgcgctggcc taccatgcag aggactgctc atcgctccct tgcgcacggt
     3001 tgagtgcgct gaagcagcgt gaaaccatcg cttcacaact ccacctgatc cgcaaatacc
     3061 ggggtggttc ggcaagcctc gctcaggcag cgctgctgct gctgcgcaac ctgctcaaca
     3121 tcccgttcta tctgctgacc ggcaaggcac gacgggcccg gcgcagcctg cacaaggtgg
     3181 ccggaacgct gctcttcatg cagcgctggg ccagcggtta cccttcgagc agccgcagat
     3241 agcgcgccac acaggcctcg accgaatagg attgcgcggc ctcgcgcaac agcccggccg
     3301 cagtgggctg atcgagcatc cgcgccatgg cacggctgag cgcgacagca tcacccaccg
     3361 gcaccagcgg gccataggct ccatcgtgca gaatctcgcg tggaccgccc gggcagtcgg
     3421 tcgagaccac cggcgtgccc accgccatcg cttcgaccag cacgttgccg aacccctccc
     3481 acagtgacga gagcacaaag agccgggccc tggccatcca ggcatagggg ttggcgacaa
     3541 aaccgggcag gctgaccgcg ccatcgagcc ccaattgcgc caccagctgc tccagcatcg
     3601 gtcgttgtgc cccttcgccc agaatcagca gccgcgcatc gtggctgcgc cgcagcaggc
     3661 cgaatgcccg cagcagggtg gagaagtcct tttcgtgact cagccgtccc gcagcgatga
     3721 tcaccggcac tgcaccggga caaaagaacg gatgctcgac cggttcggcc gcgagcgggt
     3781 ggagctgcgg cgtcaccacc ggattgccga tcaccgtgat ccgttcgacc ggcagtccgc
     3841 tcagacgggc aatgtcgcgg gccacccagt tcgatgtagc gatgatgccg tcggcctgcc
     3901 gatagagccg accgcgccgc cacgctttcc accggcggcg cagcggatga aagccgcgct
     3961 cctcgatctg ccgcgaaatg gccgttccgg ctccgaagaa gcagcggctc tccacaccgg
     4021 cacggcgccg tgcccgcaga atgaagcggt cgtcacttgg cttgccggtg atgaccgcac
     4081 gcggccggtt ctgtcgcaga tagcgggcca gcgcggcaat caccgtccgc ttcgatccct
     4141 tgccgaggcc gatcacccgc acgcccgccg ccagcggctg catgaaaggg ccggtggcat
     4201 cgctcaccag tagatcgacc gcgatgccac ggtcggcgaa tccttgcgcc agattggcca
     4261 tgttgcgttc gacaccgcca tcgcggttct ggtagatgaa gaggcagagc gggcgctgta
     4321 caccggcttc ggcggacagt tcagccatgc ggtccgcttt cttcgccatc cgacggcggg
     4381 ccatcgagcg ccagccaccc cttgagcagc gcgatcagct cggccatgcc ctcacggctc
     4441 agggtcgaga agcactggac attgacctgt tcggagatat tgacctcggc caggctcttc
     4501 agcaccttca gccggctctg ctgcgcctgg ttgcggctca gcttgtcagc cttggtgagc
     4561 aggatgcaga ccggcagcac gcgcgcctgc gcccactcca gcatcatcca gtcgaaggga
     4621 cgcagcggat ggcggatgtc catcaccagc accagcccca ccagtgacag ccgctcgcgc
     4681 aggtagtggt cgaggtgacg cttccagtgg gcctggatct ctttcgaggt ctgggcatag
     4741 ccataaccgg gcagatcgac cagacgagcc gactcgttga gcgcgaagaa gttgagcagc
     4801 tgggtgcggc ctggcgtctt gctggtgcgg gccaggcggg ccgattcggt cagggtgttg
     4861 atggcgctcg atttgccggc attggagcgg ccacaaaagg cgacctcacg accgccgtcg
     4921 gcgggacagt cgttcagcag cagtgcgctc ttcatgaaaa cggcacggcg aaaggggttg
     4981 acggcaggcg gaggttgagt cattcaccga caaaatgcag aggatgcgtt gccgggcatg
     5041 gcaagcaatg gcccctagta tataatggcc gcgatttttt tggcctgatt tgatcgtgcc
     5101 tgtcacgccg catgggcaca cggtcaaaac cagctttccg agagcctcta cgcgttggat
     5161 tcaaacatgc aaagactatt cttgaccgca gtgctgacca tcggtctgat cggcagcgta
     5221 caggcagcgg gcgatgcgac cgcaggccag gccaaggcag ccgcctgcgc agcctgtcat
     5281 ggtgccgatg gcaacagtgc ggcacccaac tttcccaaac tggccggtca gggcgagcgc
     5341 tatctgctca agcagatgca tgagatcaaa tccggtgccc gcgccgcgcc gttgatggcc
     5401 ggacagctgg acaacctgag cgaacaggag atggccgaca tcgccgccta cttcgccgca
     5461 caaaagggca cggtcggcgc tgccaaggcc gatctggtca aacagggtga agagctctac
     5521 cgcgccggca gcaagcgcaa gaatgcggcg gcctgcagtg cctgccactc tccgaccgga
     5581 cagggcaatg atctggccgg cttccccagc ctctccggtc agcatgccca atacaccgcc
     5641 gcgcagttgc aggcattccg caatggcacc cgcagcaacg atgccgagcg catcatgcgc
     5701 gacaatgctg aacttctgag cgacgccgag atcgaagcgg tggcaagcta tatttcaggt
     5761 ttgcaccctt aatcattccg ccatcgacag cgggtacaaa aagggtgtct gacacccttt
     5821 ttgtttttgg gccacccaag attccctgac cagacaagga gaaccctcat gaacaccgtg
     5881 atccgtcatc actggcgctg gatcctgtca gcactgctgc tgggcctgct gccgctgcaa
     5941 ctgcgggcgg agtcgggtta ccaggaggga gtgcactacc agaagctgga aagcgcggtc
     6001 atcacccgtg atccgggcaa gatcgaggtg atcgaagtgt tctggtacgg ctgcccgcac
     6061 tgctacaaac tggagccgac cctgcagcac tggtctgcca acctctcgcg cgatgtcgat
     6121 ttctggcgct cacccgcgat gtggaatgga cgcatggagc tgcatgccca ggccttctac
     6181 accgccgagg cgctcaacct gctcgaaaaa ctgcacaaac cgctgttcga tgcgctcaat
     6241 ccccagggtc agcagcttgc cagcaaggag gagatccgca ccctcttcac ccgccagggg
     6301 gtcaaggccg aggagttcga ccaagtgttc gactcctttg gcgtgcagag caaggtcaag
     6361 caggccaata gccgctttcg tgctttgcgt ctgaccggtg tgcccgcctt catcgtcaat
     6421 ggcaaatacc gggtgaccgg tgaaaagctc aagagcgagg atgagatgct gaaggtggtc
     6481 gattatctga tcgggcaaga gcgcaaggcg cgcgccgcgc cggccgctgt ccgctgacgt
     6541 gatcgcagga atgcacgaac accaagcgca tccgccctcc ccgcaggctg ccgaagccct
     6601 cccaaccgcc ggccagaagc tgcggttgct gacctacaac atgcaggtgg gcatcgccac
     6661 ccaccgctac cgccactaca tcacccgcgg ctggcagcat ctgctgccgc acgggctgcg
     6721 cgaacgcaat ctggagcgga ttgccgaact gattcagacc ttcgacatcg tcgcactgca
     6781 agaggccgac ggcggcagcc tgcgcagcgg cttcgtcaac caggtcgaat atctggccca
     6841 ccgcgcccga ttcccctact ggtaccagca gcgcaaccgc aacctcggtc ggctcggcca
     6901 gcatggcaat ggcctgctga cccgctttcc acccgagatt ctcgaagacc acaaactgcc
     6961 cggcgccctg cccggccgcg gcgcgatcgt ggcacgcttc gccggaccgg tcgaaccgct
     7021 gctggtggtg ggggtgcatc tgtcgctcgg cgcccgttcc cagcggctcc agctctccta
     7081 tctgctcgag ttgatgtcca attaccgcca tgtgatcgtc atgggtgaca tgaacagcga
     7141 aaccgaacgc ctgctgctcg aatcgccgtt gcagcatggc aattttcatc ggcccgcagc
     7201 ggagctcgcc acctatccga gctggcggcc ggagcgggcc atcgatcaca ttctgatcag
     7261 ccgcagcatc accatcgacc gcaccgaggt gctggacttt ccgttctccg accatcttcc
     7321 ggtggcggtc gagctgacgc tgccgagcgc agtccggctg ccggtcacgg ccaccgggct
     7381 gcattgaaat ggaccgcggc atcagaccga caggatgcag gaatgacgct gaatgagctg
     7441 cgctatctgg tcgcgctggc ccgcgagcgc cacttcggcc gcgccgccga cacctgcttc
     7501 gtcactcagc ccacgctcag cgtcgcaatc aagaagatcg agggtgaact cggcatcacg
     7561 ctgttcgagc gcaacgccag tcaggtaacg ctgaccgagg cgggcatccc gatcgtgacc
     7621 caggcgcaac gggtgcttga tgaactggcc ctgctgcgtt cactggcaca ggaaatgcgc
     7681 aatccgctgg agctgccggt caggatcggc gccatccaca ccatcgggcc ctacctcttt
     7741 cctgcactga ttccgcggct gcgtgagcag gcgccgaagc tgccgctgca gatcaccgaa
     7801 ggcaccaccg atgtgctgct ctcccagctg cgtctgggcg agctcgatct gctggtgctg
     7861 gcactgccct ttgcacagag cgggctgacc gtgcgtccac tctatgatga gccactgctg
     7921 ctggcgctgc cgcacgacca cccctggaac cagcgcgaca ccatcgacat cgatgcgctg
     7981 ggccatgagc ggatgctgct gctgggtgaa ggccactgtc tgcgccgtca ggtgctggag
     8041 ctctgcccca actgcctgct gcgcagcgag gagcgtcgtg cccagcccac actgatcggc
     8101 aactcccttg aaacgctctg caacatggtg gcggccggca acggtgtcac cgtgctgccc
     8161 gccactgcgg tgcaccgcat cgccggtgag gagcgcggtc tgatgacagt gcgtcccttc
     8221 accgcaccgg cgccacagcg gcgggtggtg ctggcatggc gcaacagctt cagccggaac
     8281 gcactgaccg acctggtggc cgaggcgatc cagcaggtga gcgcgccgct cggcgcccgg
     8341 atcaccgcag acggctgatc agcccgctct tcgcgtgccc ttcagcgccg cggcggctca
     8401 tagtcatcgg ccagcgtcag gttggccgca ctcttgccga tctcctggct gacgcccgac
     8461 ttgcccagct tgatcatcag ccgcaggtcg ttgggcgaat cggcatgggc aatggcattt
     8521 tcaaaggtga tgatgccact gctgaacagg tcatagagcg cctgatcgaa ggtctgcatg
     8581 cccagttcac gggagcgggt catcaggggc ttgatgttgt gcacctcacc cttgcggatc
     8641 aggtcggaaa ccagcggcgt gttgagcatc acctcgatgg ccgcgcggcg gccgttgcca
     8701 tcggcagtcg ggatcaactg ctgcgccacg atcgccttca ggttgagcga caggtccatc
     8761 cagatctggt tgtgccggtc ggccggaaag aaatggatga tccgctccag cgcctggttg
     8821 gcgttgttgg cgtgcagcgt acagaggcag aggtggccgg tttcggcaaa ggcgatggcg
     8881 tggcccatca cctcgcggct gcgcacctcg ccgatcatga tcacatcggg cgcctgccgc
     8941 agcgtgttct tcagcgccac ctcgaaggag tcggtgtcga tgccgacctc gcgctgggtg
     9001 acgatgcagt tgcggtgctg gtgcaggaat tcgatcggat cctcgatcga gatgatgtgg
     9061 ccatggctgt tctggttgcg atagccgatc atcgaggcca gcgaggtcga cttgccagtg
     9121 ccggtggcgc cgacgaagac caccagcccc cgcttggtca tcgacagatc cttcaggata
     9181 tcgggcaatc ccagttcacc gatttcggga atccgggtct cgatgcgccg cagcaccatg
     9241 ccggccaggt tgcgctgatg gaaggcgctg acccgaaacc gggccagacc ccgggccgag
     9301 atggcaaagt tgcactcatg gttgcggatg aagtcgagcc gctgctggtc gttcatcacg
     9361 ccaaacacca gctcgcggct ctgctccggt cccagcggcg tgcggccgat cggcaccacc
     9421 cggccattca ccttcatgct gggcggaatg ccggcggtga tgaagaggtc ggatgcgccc
     9481 tgctccacca tcatccgcaa gaaatcctcg aaattgatcg acatggcttt gttctcacct
     9541 caatgctgtg ctgtccgcac gggttcggcg ggtcagaagg cgtcaggctg cttcgccttc
     9601 tcacgggccg cctgccgggt gatcaacccc ttctgcagca ggttgcgcag cgactgatcc
     9661 atcgtctgca tgcccatgcc gccaccggtc tggatcgccg agtacatctg cgcaatcttg
     9721 tcctcacgga tcaggttgcg gatcgctggc gtcgcgagca tgatctcgtg cgccgcgacc
     9781 cggccaccgc cgacccgctt cagcagggtc tgcgagatca ccgcctgcaa cgattccgaa
     9841 agcatggaac ggaccatcgc cttctcttca cccggaaaga cgtcgacgat ccggtcgatg
     9901 gtcttggccg ccgagttggt gtgcagcgtg ccgaagacca catggccggt ctcggccgcc
     9961 gtcagcgcca gccggatggt ctccaggtcg cgcaactctc ccaccaggat gacgtcgggg
    10021 tcttcacgca gcgccgagcg cagcgcctcg ctgaagccga gcgtgtcgcg gtgcacctcg
    10081 cgctggttga ccagacactt cttgctctcg tggacgaatt cgatcggatc ctcgatggtg
    10141 aggatgtgtg aatagcggtt ctcgttgatg tagtcgacca tcgccgccag cgtggtgctc
    10201 ttgccggagc cggtcgggcc ggtgaccaga atcaggccgc gctccaccat cgagacctcg
    10261 cgaaacacct ggccgtggcc cagctcctcc atcgtcagca ccttggaggg aatggtccgg
    10321 aacaccgcgc cggcaccgcg gttctggttg aaggcgttga cccggaaccg cgccagtccc
    10381 ggcacctcga acgagaagtc ggtttcgaga aactcctcga agtcgcgccg ctgcttgtcg
    10441 ttcatgatct cgtagatcag ctcgtgcacc tccttgtgct ccagcggcgg caggttgatg
    10501 cgacgcacat cgccatcgac ccggatgatc ggtggcaggc ccgccgacag gtgcagatcc
    10561 gacgccttct gcttgacgca gaaggccagc atttcggtga tgtccatggc cattggctgc
    10621 gttgctcctc atcagaaccg aagatgatgc cggacaattc cgggcgaaat tacagcaggg
    10681 gccaaagcag ttacaattgg acacaaacac cggctctggc ctgcccattc gatgataagc
    10741 atagcggaaa acctggcggc gctgacgcgg cgaatccatg atgccgaaca gcatcacgga
    10801 cgcaggcccg gcagtgtgac gctgttggca gtcagcaagg gacaaccggc cgcgcagatt
    10861 cgcgccgccc atcaggccgg cgtgcgccat gtcggcgaga gctacctgca agaggcgctg
    10921 cccaagcagg ccgaactggc tgacctcgac ctttgctggc atttcatcgg ctcgctgcag
    10981 gccaacaaga gccgcgcggt ggccagccac ttcgactggc tgcacagcct tgatcggctg
    11041 cagctggcgc aacggctcga tgcccagcga cccgccgaac tgcctccgct caacgtgctg
    11101 attcaggtcg gcttcgaaca gcggaccggg cgggccggcg tcgcgcccga cgatctgccg
    11161 cggctggccg ggcagatcgc ccaactgccg aggttgcggc tgcgcgggct gatgacgctg
    11221 ccgctgccgc aacaggcgtg ctcgccccac ccgtttgtgc ggctggctga cctgctcgat
    11281 cggctcaatg gcgccggcca tcggctcgac acactgtcga tgggcatgag cgacgatttc
    11341 gaggcggcca tcgcagccgg cgcgaccttg atccgcatcg gcagcgcgct ctttggccaa
    11401 cggcccaccc ccttcactgc acagacccag tacggataac agatcgccca tgcaccctgc
    11461 caccgcccgc ctcgccttca tcggcgccgg caacatgtcc tccagcctga tcggcggatt
    11521 gctggaacag ggctaccccg ccagccagat catcgcgacc gaccccagtc cgcaacagcg
    11581 cgacgccgtg gccaaccgct tcggcatcga ggtgagcgcc gacaatgtcg cggcggtggc
    11641 gacggccgag gtggtgctgc tggcggtgaa gccgcaggtg ctcaaggcgg tcgcgctgga
    11701 gctggccccg gcgctcggcc accggccgct gctgctgtcg atcgccgccg gcatcggcct
    11761 ggagagcctg gccagctggc tcggcccgca gctgccgctg gtgcgctgca tgcccaacac
    11821 gccagcgctg gtacggagcg gcgcggcggc actgtgcggc aaccgccagg tgagcgcggc
    11881 acagcgcgag ctcagcctcg gcctgctcga agcgatgggc acggcgctct ggctggagga
    11941 tgagtccctg ctcgatgccg tgacggcgat ttcgggcagc ggtccggcct acttctttct
    12001 gctgatcgag gcgctgcaga tggcagcggt cgagctgggt ctgccggccg agaccgcccg
    12061 ccaactgacg ttgcagacgg ccctcggtgc cgcgcggatg gcgctggccg agccgatctc
    12121 ggcagccgag ctgcgccggc gggtcacctc gccgggcggc accaccgaac gggcgatcca
    12181 ggtgttcgag gccggcggct tcaccgcgct ggtgcggcag gcggcgctgg ctgccgcgca
    12241 acgttcggcc gaactggcca ccgagctggg cgaaccggca acccgctgaa tcaccccggc
    12301 cgcccgtcgc acaaatttca ggagtccaga atgggtcaac tctctcacgc cggcatctat
    12361 ctggtgcaaa ccctggtcag cctctacctg ctggccgtgc tgctgcgctt ctttctgcaa
    12421 ctggtgcggg ccgatttcta caatccggtg tcgcagttcg tggtcaaggt caccgacccg
    12481 ctgctgcggc cactgcggcg gctcatcccc ggcttcggtg gtgtcgatct ggcggcgctg
    12541 gtgctggcgc tgctggtgca gggcgtggcc atcgcgctga tcctgagtct ggccggtgcg
    12601 ccgctgccga acccgctgct gatcctgctc tggtcgctgg tcggtctggt cgggctcacg
    12661 atcaagatct tcttcgttgc gctgctgctg accatcatcc tcagctgggt ggcaccgatg
    12721 tcgcaccacc cgctggccga cctgctgctg cagctgaccg aaccggtgat ggcgccggtg
    12781 cgtcgcatca tcccgccgat cggcggcctc gacctgtcgc cgatcttcgt cttcatcggc
    12841 atcaatctgc tcgagatgct ggtggtcgac tcactggtca ccctgctggc gatgccgcgc
    12901 gggctggtgc tcggtctgtg agtgcgcact atcgctggga aggcgatcgt ctgctgcttt
    12961 cggtgcggct caccccgcgt gccggccatt cgcggatcat cggaccggtc gagggcgcgc
    13021 tcagggtcgg catcgccgcg gcgccggtcg atggcgccgc caacgccgaa ctgctgcggc
    13081 tgctggccag ctgcttcgcc gtgccgaagt cgcgggtggc gctgctgcgc ggcgaccgct
    13141 cgcgcagcaa gcaactggcc atcgagcggc cccaacaact gccggccgaa gccgaaatca
    13201 aggttcagta gcatgttttg ccagcacttt tcaggcacgg cgccgcattg ccgatggccg
    13261 ggccagaccc tagactgcgc gctttgcgct gcgcggtagt ctccatgccg gactccatcc
    13321 ctcccgactc ggtcggtatc gtcacccctc aacggcagcg gttcgatctg ccgctgccgc
    13381 tcgattgtgg ccgcagcctg ccgggcttcg agctggtcta cgaaacctac ggcaccctca
    13441 atgcccagcg cgacaatgcc gtgctgatct gccatgccct gagcggtaac caccatgcag
    13501 ccggctacca cggcatggag gaacgtaaac cgggctggtg ggatgtctgc atcggccccg
    13561 gcaagccaat cgacaccaac cgcttcttcg tgctggcgct gaacaacctc ggtgggtgcg
    13621 atggcagcac cgggcccagc agcctcaatc ccgccaccgg tcgcagctat ggacccgatt
    13681 ttccgatggt gaccaccgcc gactgggtgc gcagccaggc ctgcctggcc gaccacctcg
    13741 gtatcgatcg cttcgcggcg gtggtgggcg gcagtctggg tggaatgcag gcgttgcagt
    13801 ggtcggtcga tctgccgcag cggctgcgcc acgcggtggt gatcgcggcc gcccccaaac
    13861 tgacggcgca gaacatcgcc ttcaacgagg tggcgcggca ggcgatcatg aaggatcccg
    13921 acttccatgg cggccgctat ctggaacatg gcacctaccc gaagcagggg ctgcggctgg
    13981 cgcggatggt cggccacatc acctatctct ccgacgatgc gatgggacag aaattcggcc
    14041 gcgagctgca cagcgacacg ctgcgctatg acttcggcac ccagttccag atcgagagct
    14101 acctgaacta ccagggcgag atcttctccg agcggttcga tgccaacacc tacctgcaca
    14161 tgacccgcgc gctcgactac ttcgatctgg ccaaggcgca tggcgatgac ctggtggcgg
    14221 ccttccgcca ggcacggtgc agctttctgg tggtgtcgtt caccaccgac tggcgcttct
    14281 cgtcggcccg ctcgcgggag ctcgtcactg cactggtgga ggccgagcgc aacgtcagct
    14341 acgccgacat cgaggcggcg caggggcacg atgcctttct gctgccgatt ccgcgctaca
    14401 tggagctgct gcgcgcctac ttcgaccgca ttgccaacga atcctggccg gtggcggcgg
    14461 catgaggccc gatcttggca tcatcatgga gtggatcgcc gccggctcgc gggtgctcga
    14521 cctcggctgc ggcgatggca ccctgctggc cgagctgacc cggcagaagg gggtgcatgg
    14581 ctggggcatc gagaacaatc cgcacgacat cagccactgc atcgcccaag gggtcaatgt
    14641 catcgagcat gacctcgacc agggtctggc cgaattccgc acccaacgct tcgacaccgt
    14701 ggtgatgtcg ctggcgctgc aggcggtccg ctttcccgat ctgatcctcg acgagatgtt
    14761 gcagatcggc cgtgaatgca tcgtcacctt tcccaatttc ggccactgga gctgccgcca
    14821 cgacatcggg ctgcgcgggc ggatgccacg ctccgatttt ctgccccacg agtggtacaa
    14881 cacccccaac atccacttct gcaccatcgc cgacttcgag gcgctgtgcc gcaagaagaa
    14941 cttccagatc ctcaatcagc gggtggtcga tcgccactac cgcagcaatc cgctgatgcg
    15001 acgctggccc aacctgctgg ccgaaatcgc catctaccgt ctgacccgct gagagctcga
    15061 ccatgcacca ccggattctg cttctgctgt cattgatgct gctcggtgtc cagctggccc
    15121 aggccgagca gatggtccgg ttcgatggct acgagctgca ctacaacgtc agccccagcc
    15181 ggctgttgac cacaccgacc gctgcccggc tcggcatcgt gcaggccccc aaccgggcgc
    15241 tggtcaatgt caccgtgctg aagcttcagg ccgacggtcg cagccagccg gtgaccagtc
    15301 aggtgcaggg ccaggcgcgc aacctgatgg ggcagagcgt gccgctgcaa ttcagaacgg
    15361 tcatcgaaag tgaccagccc tccgcgatcg ccgagctgcg gttcgatgat gaagagctgt
    15421 tccatttcga actcgaggtg accccggccg gcagtgaccg tggctatccg ctgcgcttcg
    15481 accagcagct cttcatcgaa tgatcaacga ggactcgggc cagcgcatgg atcattcagc
    15541 cacttttcgg caacggatgg tgctggccag caacaatgcc ggcaagctgc gcgaatttgc
    15601 cgatctcttc gccggtctgc ggatcgagct gctgccacag tcggccttcg cggtcgccga
    15661 ggtcgaggag agcgggctca ccttcgtcga aaatgccctg ctgaaggcgc gcgctgcctc
    15721 tgtcgccagc ggcctgccgg cggtggccga cgactccggc ctggcggtcg acgcgctcgg
    15781 tggcgcccct ggactctact cggcacgcta tgccggggcc ggcgccggcg atgcagccaa
    15841 caatgccagg ctgctcgctg cgctggccgg ggtgcccgag gcgcaacgga ccgcccgcta
    15901 ccactgcctg ctggtgctgt tgcgccatgc cgaggatccc gcacccctga tcagccacgg
    15961 catctgggag gggcgcatcc tgactgcagc gcgcggtgcc ggcggcttcg gctacgaccc
    16021 gctgttctgg gtcgaatcgg agcaggcgag cgccgccgag ctcgccccgg cgcgcaagaa
    16081 ccagttgagc caccgcggtc aggcgctgcg ccatctgctg cgccagttgc agccatgaag
    16141 cgggagacca cgccgcgccg cttcggccgc ctgccgccgc tcgggctcta tgtgcacatc
    16201 ccctggtgtg tccgcaagtg tccctactgc gacttcaact cccatgccgc cgacggcagc
    16261 ctgcccgaag cggactatgt cgaggcgctg ctcgatgacc tgctgcagga tctgccgctg
    16321 gcgcaggatc gcccgctgca gagcctcttc atcggtggcg gcactccgag cctgttcagc
    16381 gccgacgcca tcgcccggct gctggagggg atcggcgcgc tgctgccctt cacccgcgac
    16441 atcgagatca cgctcgaggc caaccccggc gcggccgacc aggcgcggtt ccgcggctac
    16501 cgggccgccg gggtcaatcg gctgtcgctc ggcatccaga gcttcgacaa tgacaaactg
    16561 cgcgccctgg gccgcatcca cgatgccgag gcggccgacc gggcgatcga ggcggcccgc
    16621 gcggccggct tcgacaactt caacctcgat ctgatgttcg gattgccaca acagtcgatc
    16681 gaagccgcgc tggccgacct gcaccgcgcc atcgaccatc gcccgactca cctcagctgg
    16741 tatcagttga cgatcgagcc caacaccgcc ttccacaagc ggccgccgcc actgccggat
    16801 gacgagctgc tctggccgat gcagcagcag ggccagcggc tgctcgccgc cgccggtttc
    16861 gagcagtacg aaatctcggc ctactgccag ccgcagcgcc actcgaaaca caacctcaac
    16921 tactggaagt tcggcgacta cctcggcatt ggcgccggcg cccacggcaa gatcaccctc
    16981 ggcagcgcgc agcaggtgat ccgcacccgg aagagccgcc agccgcgcca ctacctcgac
    17041 caccagaaga gctacctggc cggcaaacag gtgctgaacg aggccgatct gccgcttgag
    17101 ttcctgatga acgggctgcg gctcaacagt ggcgtcagca gtcagagctt cgtgcagcgc
    17161 accgggttgc cgctcagcag catcgacgac gcgctgctgc aggcccggga gaagggattg
    17221 cttgaagagg agagcacgcg cctcaacgcc accctgcgtg gccggcagta cctcaactcg
    17281 ctgctggagc tgtttctgta ggctgtccgg ctctcggaga tcagccgtcg cggtcgctgc
    17341 gttcgaacag cagatcccac acgccgtggc ccagccgctc gccgcgccgc tcgaacttgg
    17401 tgaccggccg ccacggcgga cggggcacga agccgccatc ggcagcgaga ttgcgccagc
    17461 cggacgcctg ctcgaggctg agcagcatcg cctcggcgta gggttgccag tcggtggcga
    17521 tgtgcagttg gccttgcggt cgcaccacgc gatgcagcag atcgagcagc gctgcctgca
    17581 ccagccgccg cttgtggtgg cgcttcttcg gccaaggatc gggaaagtag atctgcaccc
    17641 gcgtcaggct gccctcgggc atgacgccgg catgcagcat cgccatcaca tcgccgcgca
    17701 gcagcctgag gttgccgagc cgctcctgct cgatgcgctg cagcaggttg ccgatgccgg
    17761 gcggatgcac ctcgacaccg acaaaatcac attccggggc gttctgcgcc atcgtcacca
    17821 gcgactcgcc catgccgaag ccgatttcga gcaccaccgg cgcggtgcgg ccgaagcagg
    17881 tcgtccagtc gagcggctgg tcaggctgcc agtcgatgcc gtaatgcgcc cagagctgct
    17941 ccagcgcccg ctgctgcgcc gggctcagcc ggccgccacg gatgacgaag ctgcggattg
    18001 cgcgaagatc gcggtcactg gccggaaccg acatcgaatc cacggtgcga gccgtcagaa
    18061 aaagcggcca tcgagcggcg aagaggcact ggcataacgg cggcgcggca tgcggccggc
    18121 cagataggcc tcgcggcccg cctccacggc cttcttcatt gccgccgcca tccagaccgg
    18181 ctgcgtcgcc tcggcgatcg cggtgttcat cagcacgccg tcacagccca gctccatcgc
    18241 aatggccgca tccgaggcag tgccgacacc ggcatcgacc agaatcggta cggtcgcgtt
    18301 ctcgacgatg gtgcggatgt tgtaggggtt gcggatgcca aggcccgagc cgatcggcgc
    18361 gccgagcggc atcaccgcga tgcagccgag ctgttcgagc cgctgggcga tcagcggatc
    18421 gtcattggtg tagaccatca ccgcgaagcc ctccttgacc agcgcctcgg cggcaatcag
    18481 ggtctcggtc acattggggt agagcgtctt ctcgtcaccc agcacctcca gcttgaccag
    18541 cgccgccccg tcgagcagct cccgcgccaa ctggcaggtg cgcaccgcat cgcgggcact
    18601 gtagcaaccg gcggtgttgg gcagcagggt gtagcgcgtg ggtggcagga tgtcgagcag
    18661 gctgggcgca tcggcctgct gcccgatgtt gacgcggcgc acggcgaagg tgacgatctg
    18721 ggcaccactg gcctcgaccg cccgccgggt ctcatcgaaa tcgcggtact tgccggtgcc
    18781 gaccagcaga cgggagtggt aactgacgcc accgatcagc agtggatcct gttgcggttg
    18841 aattggctgg gtgctgtcca tccgatacct tcacctctat tcagccgcca ccaatggcgg
    18901 tcacgacctc gatctggtct ccggcctgca ctgtctgctg ggcatggagc gtgcgcggca
    18961 ctattgtacc gttcagttcc accgcgatgc gccgctgctg caacgccagc cgctcgatca
    19021 gcccggcaat ggtcaggccg gccggcacct gctgcggcac gccattgagc tggatggtca
    19081 gttcagcact gctcaccgca agcctcgcca gagcagatgg gccagcagca gccaaccggc
    19141 aatgaatgcg acgccgccca gcggcgtgat gaaaccgagc tggcgcacgc cggtcagggc
    19201 cagcaggtag aggctgccgc tgaaggccag cagaccgaac agaaacagcc agccaacccg
    19261 atgcagtccg gccacctccg gcagcagccg catggccaga ccgacggcga tcagcgcgag
    19321 cgcatggtag aagtggtact ggacggcggt ggcccaggtg tcgagctggg ccgccgcgag
    19381 ccgtgccttc agcgcatggg cgccgaaggc ccccgtcatc accgccagtg cgccgctcac
    19441 ggccccggcc accagccagc ctctcgccgt catcgccatg ctccgcttgc gttcaacgca
    19501 acaggccgct gaatgcggcc tgttggcatc ggctggcagc atgcatgctc aggcgtcgat
    19561 ggcactgcgc agccgcttca tcgcattctg ttcgagctgg cggatgcgct cggccgagac
    19621 gccatggatg gccgccagat cgtgcagggt cgccttcggc tcattcagcc agcgccgttc
    19681 gagaatgtcg cggctgcgcg gatcgagcgc ttcgagcgcc atcagcaact gctcgccctg
    19741 gtggctgcca tggtcgagct gctcgaggat cagtgccggg tcggcgctgc tgtcctcgag
    19801 gtactcggcc ggtgccggcg ggctggagtc gtcgtcatcc tgtgcatcga acgaggtgtc
    19861 gtgggccgcc agccgcccct ccatctgccg caccgtcttc gggtcgacat tgagctcgcg
    19921 ggcgatgctg ctcacctcgt cgttgctgag ccagtcgagc cgcttcttgg cgccgcgcag
    19981 gttgaagaag agcttgcgct gcgccttggt ggtggcgatc ttggcgatgc gccagttgcg
    20041 caggatgaac tcgtgaatct cggccttgat ccagtgcacg gcgaacgaca ccagccgcac
    20101 ccccatgttc gggtcgaacc gcttgaccgc cttcatcagg ccgatgttgc cctcctggat
    20161 caggtcggcc tgcggcagcc catagccggc ataaccccgg gcgacgtggg cgacgaagcg
    20221 caggtgcgac agcaccagtt gccgggcggc gtcgaggtca ccctcttcgc tgtactggcg
    20281 ggcgaggcgc tgttcatctt cctgcgacag catcggaatg cgctgcaccg cctgaaggta
    20341 ggcgttgagg ttgccaaccg gcgccagcac gttcatgggc agaatgtcgg tactcatgat
    20401 cacctcttgc agatcggctg agaattctag cacttggccg attggagtgc taaaaccggt
    20461 cgaagttcca agtgattcaa tcaggctcga tcgagcgcag ttgccgctcg gccgcgatcc
    20521 aggcgcccgc cacaccgagc agcgcactgc ccagcagcag gatcgtcgtg ccatgcatcc
    20581 ccaatcccac cggcgtgaag ctgctctgat agagggcggc cagctcctgc accggcgcgc
    20641 gcagccagag cagcagcgcc ccggtcagca gcaatgccgc catgccgccg cccagaccat
    20701 accagaggcc ggtgtagaga aacggccggc ggatgaaggc cgcggtgcca ccgaccagtt
    20761 tgatcacctc gatctcgcgc cggcggttgg tgatggtcag ccgcaaggtg gtggcgacga
    20821 tcagcagcac cgccaccgcc agcagcagcg tcaaggccag taccgcccgc tgtcccagct
    20881 cgatcagcgc cagcagccgc gccagccact cggtgtcgac ctgcgcctgc tcgacctccg
    20941 gcagcgtggc caaccgccgg gccagtgccc gcagcggctc gatcccaccg ctgtcggccc
    21001 gcggcgtgat caacagcagc ggcggcagcg ggttgctgcc gagcgcgtcg atcacatcat
    21061 ccagtttcga gcgctgccgg aactccagca gcgccgcctc gcggccgatc accttgaccg
    21121 cggcgatctc ggcatcgcga cccagccgct caccgagcag gcgggccatc tcgtcactct
    21181 gctcgatctt gaggtagagg gtgatgcggc ggctgccttc actccagccc tcgctcacct
    21241 gcttgaggtt gtcgagcaga agatagaggc cgctcggcag cgccagcgcg atgccgacca
    21301 tgctccagat catcagcgtc gccatcggtt cggccagcaa ccgcctgagg ctgtcccggc
    21361 agtggctgcg gtgctgcagc acgtaaccgc gcaggcggtc acgccagccg ggcggctggc
    21421 ggcgggcgga gttctggcct ctcgccatct tggcactcat ggcggggcag cgtcgccgtc
    21481 gtcgaacggg tcgttgatca accggccttc gtgaagttcg aagaccggtt tgtccatcgc
    21541 atcgatcagc gcggtgtcgt ggctggcgat cagcaccgtg acccccgcct ggctgaaggc
    21601 ggagaagagc gccatgatct cggcggagag gtcgggatcg aggttgccgg tcggctcatc
    21661 ggccagcagc agcggcggcc tgctgaccac cgcgcgggca atgccggcac gctgctgctc
    21721 gccaccggag agctggatcg ggttcatccg ctccttgtcg agcaacccca ccttgtcgag
    21781 ggcggcccgc acccggcggc cgatctcgtg ccgcgaagcg ccggcaatca ccaacggcag
    21841 cgcgacattg tcgaacagcg tgcgatcgaa cagcagccgg tggttctgga acaccacgcc
    21901 gatgttgcgg cgatagaaag gaatgctgcg ctgtggcaga cggtcgagat cgatgccgcc
    21961 caccagcacc cgcccctggg agggccgctc catgcgcatg atcagcttca gcagcgtgct
    22021 cttgccggca ccggagtggc cggtcagaaa ggccagcgac cgtttcggca gatgaaagga
    22081 gaggtcgcgc agcgcctcgt ggccaccttc atagcgtttg ctgacctgct cgaaccgcag
    22141 catcgatcag gccggatcgc gctcgaacag tgcgcggatg aactcatcgg catcgaacgg
    22201 acgcaggtcg tcgatcgcct cgccgacgcc gatgaagcga atcggcagac cgagctgctg
    22261 ggcaatggcg aagatgacgc cgcccttggc agtgccatcg agcttggtca gcgcgatgcc
    22321 gctgacatcg accgcatcgc gaaactgctg cgcctggttg agggcattct ggccggtgct
    22381 ggcatccagc accagcagca cctcgtgggg cgcctcgggg tcgatcttct tcagcacccg
    22441 caccaccttc ttcagctctt ccagcagatg cgccttgttg tgcagccggc cggcggtgtc
    22501 ggcgatcagc agatcgatgc cgcgcgcctt ggcggccgac agcgcatcga acagcacgga
    22561 ggcgctgtcg gcaccgctct gctgggcgac caccggcacc tggttgcgct cgccccacac
    22621 cttgagctgc tccaccgccg ccgcgcgaaa ggtgtctccg gcggccagca tcaccttctt
    22681 gccctgctgg ccatatcgtt tcgccagctt gccgatggtg gtggtcttgc cgacgccatt
    22741 gacgccgatc accagcagca cgtaggggcg cttgctgtcg tcgatctgaa gcggcaggct
    22801 gaccggctgc aggatctcgc tcagcgcggc acgcagcgcc gcatggaggg catcggcatc
    22861 ggccagctgc ttgcgcgcca cccggtcgct cagttgatcg atgatctgcc gggtcgccgc
    22921 catgccgaca tcggccagca gcaactgctc ttccagcgcc tccagcagct cggcatcgat
    22981 ctgcttgcgg ccgagtatca gattgctcag tccctcggcc agcgagacac gggtgcgggt
    23041 cagaccactg cggatgcggc tgaacagccc cggcttgctg gcactcggct cggtctcggc
    23101 gggcgcggcc accgtgggag cagcctgcgc ctgctgcggt tcagtatcgg cagtcggcgc
    23161 tggttcgtgc aggagtgcat cgatgggtga cggcgacgat tcaggcgctt cagcgtcatg
    23221 ctccgcggaa ggcgacgccg tgcccgatcc cgccgcctcg ggcgtctggg gggtgttgtt
    23281 gcgcttgagg aacttgaaca tgacgccgat cgctggagtg gaaacgggcg cgctggcggt
    23341 gactcgcgcg ggttgggtat cctagcaccc cttgccccgt tcggggtatg cagagcgcag
    23401 aggtctgttc gatgttgcca gcccctttcg acggcctccc gccggcctcc tcgcggtggt
    23461 gaatccagcg cacacggccc ccggacgcat ccgcatcatt gccggccgct ggcggggacg
    23521 acgcctcccg gtgatcgcca gcgagatgct gcgccccacc cccgatcggg tgcgcgagac
    23581 cctgttcaac tggattgggc ccgccgtgcg cggcacacgc tgcctcgatc tgttcgccgg
    23641 caccggcatc ctcgggctcg aagcgctgtc acgcggcgcc gcgcaggtgg tctggatcga
    23701 ggcggatatt cggctggtca gggcgctgcg gcagatccag gccacgctgc aagccggcgg
    23761 cgaggtggtc tgtggtgatg tgaccgactg gctgaccggc ttcgacccag cgcgctctgc
    23821 acccttcgac ctggtctttc tcgatccgcc cttcagacgc ggtctgctcg aacaggtggt
    23881 gcagcaactg gagcagcgcg ctctgctgag cccacaggca ctgatctacc tggagcacga
    23941 gcgggagcat cagcctgcac tgccgccgaa ctggctgccg ctgcgcgcca cccacgccgg
    24001 tcaggtgacg gcgcaactgc tacggcggca gccggtggcg cagtccgatt gagcggccgc
    24061 gaaccgccgt agtaacatcg ccactccatc tggcagtgca gcgaagagaa taaccatgaa
    24121 aaccgccatc tatcccggca ccttcaaccc tctgaccaac ggccatgtcg acattctgga
    24181 gcgggccgcc cggctgttcg accgggtgct gctcggcatt gcgcccagtg ccggcaagca
    24241 gccgctgttc acgctgcagg agcggatcga cctcgctcgc ctctgcgtcg cccatctgcc
    24301 caatgtcgaa gtgctggcca tcgaggggct gctgatcaac ttcagccgcc agcacgatgc
    24361 catcgccatc gtgcgcggga ttcgcgccgt ctccgactac gaatatgagc tgcaactgac
    24421 caacatgaac cgggcgatgg cgccgcagat cgagaccatc tttctgaccc cggccgacaa
    24481 cctgtcgttc atctcatcga ccctggtgcg cgagatcgcc agctttcatg gcgatgtcag
    24541 caagtttgtc cggccggagg tcgccacggc attgcaggcc aagtttccgc caccgaaata
    24601 gcaacgcacc ggcagccggc atggccttga tgatcaccag cgcctgcatc aactgcgatg
    24661 tctgcgaacc cgagtgcccc aacggtgcca tccatgccgg cgagctggtc tatgagatcg
    24721 acccggcgct ctgcaccgag tgcgtcggcc atttcgatcg gccgcagtgc atcgagatct
    24781 gcccggtcga ctgcatcctc accgacccgg agtgcatcga atcgccgcag gcgctgcaac
    24841 ggaagttcca gcaactgcac ccctccaccg agccctgagg cgctggacta ctgcgtcggt
    24901 ccgtcgccga cggctggtgc aggcttgcgg gaggcggcgg tgcagcccag gcagcggaca
    24961 aaggcgttcc tggccggacc ggccaccaac tgatcggccg cctcgtcgac attgccgccc
    25021 agcgccgaaa agggcaggct gacgatgaac agcccagtgc ccaccaccgt catgccgatc
    25081 agcagcggcc gtgccaccag cagatcggcc gccatcgcgc cggcacccgg ccggttgccg
    25141 tcatcgctgc tgccggccca cagcggctgg gtgccgagca gcaggctcag cgtcagaatc
    25201 ccggtctggc agagagaccg ccggttgcgc aggttcatct gaactctcca tgcaggttga
    25261 agccaagaac attcgataag cctggtccat ggtagcgcaa tggcgcaccg atgctactcg
    25321 gccggcgcag caccgagcca ggcccgggtg cgctgcacgt agcgctcctt cagcgtcagc
    25381 acgcgcttct ccagcagatg ccaggagagc gcgctgagca gcagaaccac cggcaccgca
    25441 atcagcacca tctccagcac cgagacgtcg ggtatcaatg cgaccaccgc ttgctgcacc
    25501 gggaaggcat aaatatagaa gccgtaggag taatcaccca cccggttgta gccacggacc
    25561 agacccgaag gcacataggc cagatagagc agcaggtagg cgatcgagcc atggtagagc
    25621 acaaaaaaga ggtggcggtg ctcgaccgac agcaacaaca gcagaagcag tggccagaac
    25681 agcgcgccgt gcaaacggat gcgcgcccgc agcacataaa aggtcgcacc actgaagaac
    25741 atgaagatga atctggcaag ctggtggagc cgcggagatt gatcgatcaa ggcaaactcc
    25801 tcggccagca gatagcccgc cgcgagcagc gcatagacga tgaccgcccg ctgcaatgcc
    25861 ctgatgcgat cgctccgcca gcgcaacagc agccaggtga cggccagaat ggcgtacatc
    25921 cgcacttcgt aaggcatggt ccagagtgac ccattgacgg cattcggcag cggattggtt
    25981 gtgaacacac cgggcagcgt agaggccgtg tggatgaaga ccgtggccgc cttggccagc
    26041 cgcacataga tcgagctctg cgcaaagtag tccgacggcg gcaaggtggt gaagagcggc
    26101 cccagcacga acgccatcat cagcagcatc agccagtaag ccggatagat ccgcagaaag
    26161 cgggcccaga caaagtcgac gccgttttgc cgcttcagca gactggcagt gaccagaaac
    26221 ccgctggcga cgaagaagag atcaaccgcc agatcgccca gtgtcaccat gccgatcagc
    26281 cttttgaccg ggtcttcgcc cgagccggtc agcgcaaagc tgtggctcac gagcaccgag
    26341 caagccgcca ccatgcgaat gagattgaag ttgttgtcgc ggctctggct gcggtcggag
    26401 agcttcactg ctggcactcc tggcaaaaaa aggtggagcg ctggttgagc cgtatcgagg
    26461 ccacctggcc cccgcagcgc gggcagggct gcccggtgcg tccgtagaca tcgagcgcga
    26521 agccgaaata gccggcctgg ccatcgctgt tcagaaagtc gcgcagcgtg gtgccgccct
    26581 gggtgatcgc ctgctccagc agccgcctga tctgcccgac cagccgctcg cagtcggcat
    26641 gggagagggt gccggccggt cgtcgcggat cgatgccggc tcgatggagc gcctcgttgg
    26701 cgtagatgtt gccgatgccg accaccactt cgctctgcat cagcaggctc ttgatcgcca
    26761 ccgtgcggcc acgtgcgctc cgatggaggt ggccgccgtc gaacgcctcg ccgagcggct
    26821 ccggccccag cctgctcagc aggggatggg tcagcggatc gccctcgagc cacaggcagc
    26881 agccaaagcg gcgcggatca cggtagcgca gcagcgcgcc gccttcgagc agcaggtcat
    26941 agtggtcatg tgccgtcggc gcgacggcgg gcggttcgag cagcatgaag cggccggaca
    27001 tgcccagatg gagcagcagg tgtccgccat cgagttgcag cagcagatac ttggcgcgcc
    27061 gctgtaatga gccgatgcgc cggtcgcgga ggcgttcggg cagctcatcc ggaatcggcc
    27121 agcgcagccg gccattgcgc agcaccatgt cgtggatgcg gcggccaatc agatgggggg
    27181 caaggccacg caaggtggtt tcgacttcgg gcagttccgg catgcaaaaa cggctgggta
    27241 aaatggcggt gagtgagcgg ccgtcacctt agcagccagc gatcgacaag gggagagtcc
    27301 agacacgatg cgcgacatga tggttctggg aatcgacacc ggcggcacct tcaccgactt
    27361 cgtgctgttc gatggcgaac ggctgcatat ccacaaggtg ctctccacgc cagaggcccc
    27421 cgacgaagcc atcgccaccg gcatggccga actggggctg gaagcgccac tgcgccaggg
    27481 caagctgctg ctggtccatg gctccacggt cgcgaccaac gccgcactgg agggcaaggg
    27541 ggtgccgacg ctgttcgtca ccaaccgtgg ttttcgcgac ctgctgacca tcggccgcca
    27601 gatccggcgc gagctctacg aactgatgcc ggcgccgctg ccgccaccag tgcccgccga
    27661 gctctgcttc gaggtcgaca cccggcgcga ccaccttggc cggctgatca cgccgctgac
    27721 cgacgccgat ctgacccggc tgcgccacgc catcgccacg ctggcgcccc aggcggtggc
    27781 gatctgcctg ctctacagct tcatggatga gagcgaggag caggcgctgg ccgccgcgct
    27841 gccgccggga ctgttcgtct cctgttcgtc gcaggtgctg cccgagtacc gcgaatatga
    27901 gcgcggcatc gccacctggc tcaacagttg gctcggcccg ctggtgtccg actatctggg
    27961 ccggctcagc gcacgaacgg cgccggcacc gctcaccatc atgcagtcat cgggcggtac
    28021 cgtcgccgcc gaacaggccc ggcagcgcgc cgtcaacctg ctgctgtcgg gtccggccgg
    28081 cggcatggcg gcggcacgcc atctggccga caccctcggc gaacggcagt tgatcacctt
    28141 cgacatgggc ggcacctcga ccgatgtcgg cctgctcagc gggggcgagc tgacgctgac
    28201 caatgagggg cggatcggcc cctatccggt cgcggtgccg atggtcgatc tgcacaccat
    28261 cggtgccggc ggcggatcca tcgcctttct cgatgatgga ggcggactct gggtcggacc
    28321 gcgctcggcc ggcgcactgc cgggcccggc ctgttatgga cgcggcggcc gcgaaccgac
    28381 cgtcaccgat gccaacctgc tgctgggccg gctgcggccg gaacggtttc tcggcggcgc
    28441 catgccgctc gatgtcgcgg cggcgcagcg cgccatcgcc acgctggccg atccgctggg
    28501 actgtcgctc gaagagaccg ccgccggcat catcgagatt gccaatgagc agatgattcg
    28561 cgcattgcgg ctgatctcgg tggagcgcgg ccacgatccg cgccagttcc ggctctgctg
    28621 cttcggcggc gccggcggtc tgcacctgtg ccggctggcc gaggcgctcg gcatccgccg
    28681 cgctct
//
LOCUS       DAORCQ010000024        28202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_919_length_28202_cov_5.966213, whole genome shotgun sequence.
ACCESSION   DAORCQ010000024 DAORCQ010000000
VERSION     DAORCQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 28202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28202
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_28202_cov_5.966213"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKI23_04110"
     CDS             complement(<1..674)
                     /locus_tag="PKI23_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN36160.1"
                     /translation="MKQTIQHTQLSPEQAAQCGMGLAIHARQTPQRLAIQSEHGVRTF
                     DALNASANQLLQALRRRGLRPGDAVALLCANRPEFPETLIACMRGGLRVTPVNWHLTP
                     REISYIVDDCEARVLIGDARFTEALQQVKASCNQALIGLAVGGDIAGFDRYDAALEGE
                     STADPDDAVAGSMMLYTSGTTGHPKGVQRPPNAPPNHQLVGMLLHADYQPGVSLDLCT
                     GPLYHAA"
     gene            958..4821
                     /locus_tag="PKI23_04115"
     CDS             958..4821
                     /locus_tag="PKI23_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNN36161.1"
                     /translation="MSVDSVSPWQQVRALQTALFNGVQPQKLLAEALQLLVEQMQVRI
                     GTLLRVERQHDGLYRLEPLCCHQQGQNGPDRALPPFLSLNGDEQPLLQRMLMAETVQR
                     WLAADCPVSLTAKLGHADPCDSVSTLTLHHGGEPIALLWLVDPSADPLPADGSALELL
                     RLTLIHLLLDLNRRRQQQRRHKLAEGCTGALTALAQQRPIEEILTLLADTLVRLFPDR
                     GCLFMRLNRKRDRLEPVAWAGLSAAHVAAVRDFPVDARSICCGLAAWRGEEVMVDSLE
                     EVLTDAPLRGVIIGSGFQACWSRPLLDAAQRVAGTLAIYGRSPLHLSEGDRRMIEQVT
                     QVAALTLSHWTALELLRERDQMLAGAFDRAVHPMALCDTQANFLQVNDAFCDWLGYPR
                     EQLLRMSVFDIVFPDALSSAREILQRGSAERAFRADERRYRHRDGSVRHAAVSISVVV
                     DQHGTFSSAVLDFFDLTPHRQMLDTLRQREAELLASQRLARLGSWRLEVASGHLSYNQ
                     EAMKILGSDPRVTEALTTLSGFQQLVHPADRSRLAEALEATLRDLQPYQIEYRFLLPE
                     GRLLWVDSRGEVELAADGQLVAVSGVFQDVTERKRVEESLRASEARFRGTFESAAHGM
                     AITSPTGHFIQVNQSLARILGRAPEALIGRPVADFAPPQDLERDREIIRQFSSAKLRQ
                     LTFDKRYLRPDGHLVWATLSVAGVYDQNGALQHFITHTLDVTERKQAEAALDEERERL
                     FVTLNSIGDAVITTDAEGRVTFLNPVAEQLTGWYNTEVLGRPLEQVFHIISEDHRQQV
                     ESPVQRVLLEGRIVGLANHTLLINRLGQEIAIEDSAAPIRNLAGDIIGVVLVFHDVSQ
                     ARQMAVEMSWQATHDALTGLVNRREFEHRLSEAIKRCWQEQVTHIFLYLDLDQFKLVN
                     DTCGHLAGDELLRQLTHLLHGRIRDIDTLSRLGGDEFGVLLEHCSLEKGLEIADTLRT
                     LVREFRFVWEEKIFEVGVSIGLVLIDEQIESADKALSLADLACYAAKDLGRNRVHVYQ
                     ADDHELARRHGEMQWAARISRALAENRFTLYRQQIRPVRDEQREPLHFELLIRMLGEQ
                     DELILPSQFIPAAERYNLMPNIDRWVVEHCFRYLSRHHDGQTRFTINLSGMSLGEEAT
                     LDFIRERMRHYQIDPLCLCFEITETAAIANLVKASRFINSLKSLGCRFALDDFGSGLS
                     SFAYLKNLPIDYLKIDGSFVKDMVDDPIDRAMVSAIHQIGQAMGIRTIAEFVENGAIL
                     EQLRAIGLDYAQGYAIAWPEPLPNF"
     gene            complement(4910..5833)
                     /locus_tag="PKI23_04120"
     CDS             complement(4910..5833)
                     /locus_tag="PKI23_04120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNN36162.1"
                     /translation="MLRRCRSIVLMMALLGSTPLFAASYSQLVVIGDSLSDTGNLYDL
                     TASLPPIFGIPATPPPPYSNGRFSNGPVYVEYLSSSLGLGAVTPSRQGGTDYAYGGAL
                     VSAAALGIIPSLSSQRTALFTDTGGSLDSNALYVVWGGNNDLFGLLGQSLTPHDAQAA
                     VEGVASLLVSVVGDLVAHGAQHLLVPNIPDLGMTPAELGNADATQYSQWFNSQLSSGL
                     ASLQADIELFDTFSTLQQLAPTFANATSGCIGLPAGCAGYLFFDDKHPTTAAQQILAD
                     RMYAQLTAVPLPAGVWLFGSALTLLLVRRRG"
     gene            complement(5913..6842)
                     /locus_tag="PKI23_04125"
     CDS             complement(5913..6842)
                     /locus_tag="PKI23_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN36163.1"
                     /translation="MGLASSRVEPPPVLEFGRAQVLMGRNGGKFPYGNSILVRGSEES
                     LIIDPSKSLKYHSVDLAGVDRVLLSHCHQDHVAGVHLFPDSLCQAHPLELPAIQGIDG
                     LLELHGVVDEEREQFSQNVRDYFNYTPRPDATAFEVERELDLGRVKVQFLHTPGHTHG
                     HTCFIVRGEQRRHELIFLADIELTGFGPYYGGPNSSLVDFDRSLTEVRQLDLAWFVTY
                     HHIGVLDQPAFRARAAQYHGMIAQREARLLDYLSEPHTLEEAVAHRIVYRPADTGHHT
                     DRNERNMLRQHLDRLLEQERVIEVAPGRYQRRS"
     gene            7015..7668
                     /locus_tag="PKI23_04130"
     CDS             7015..7668
                     /locus_tag="PKI23_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNN36164.1"
                     /translation="MSDQAIVSTQRAQALTASSKVLRNTYALLALTLLFSALTAALSM
                     AVNAPYFGLLSLLPYFGLLYATEKSKNSGWGLVWVFALTGWLGFTLGPILNHYVGLRG
                     YEPVLLALGGTAAIFLSLSAYVLVTRKELSFMTGFLAMGMLVAFIAAIANAFLQIQAL
                     TLAVSCMFLLLSSALIMWQTSAIIHGGETNYISATVGLYVAIYNLFTLLLGFLGQDD"
     gene            7732..8124
                     /gene="tusD"
                     /locus_tag="PKI23_04135"
     CDS             7732..8124
                     /gene="tusD"
                     /locus_tag="PKI23_04135"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HNN36165.1"
                     /translation="MRFAIAIHGAPFSHQGAQSAYRFALATLAAGHRIGRIFFYQDGV
                     HNGNRLITPAQDELNLPDCWQTLAEQHEIELVVCIAAAARRGVLDQREAARHQHPAAN
                     LAPGFTLSGLGQWIDALAESDRFIAFGP"
     gene            8139..8492
                     /gene="tusC"
                     /locus_tag="PKI23_04140"
     CDS             8139..8492
                     /gene="tusC"
                     /locus_tag="PKI23_04140"
                     /inference="COORDINATES: protein motif:HMM:NF001238.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HNN36166.1"
                     /translation="MKLAIVQTRPPHQQASGREGLDTALVGAVFDLEVSLLFVDDGVY
                     QLLAGQDPAAIGQKPWCAAFEAAELYGIRQLLVDAEALAERGIEVGELMVPVELRSGS
                     ELAQHLDSCDQVLVF"
     gene            8498..8806
                     /gene="tusB"
                     /locus_tag="PKI23_04145"
     CDS             8498..8806
                     /gene="tusB"
                     /locus_tag="PKI23_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HNN36167.1"
                     /translation="MPSLHTLNKSPTAAPLLRRCLRLAQPGSTIVLIEDAVYAAVDGS
                     PGAALLRAHHDRFTFHALQADLQARGLVGRRLVEAVGVIDDADFVALVVQHPRIISWY
                     "
     gene            complement(8831..9406)
                     /locus_tag="PKI23_04150"
     CDS             complement(8831..9406)
                     /locus_tag="PKI23_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNN36168.1"
                     /translation="MQAFLISTGVVALAEIGDKTQLLAFILAARFRRPWPIVLGILLA
                     TLFNHGLAGALGSWITAQLSPQSLRWLLGGAFLAMAAWVLVPDRFDEGEVASSRFGVF
                     GTTLLTFFMAEMGDKTQIATVALAAQYQSLIAVVLGTTLGMMLANVPAVLLGERIAQR
                     LPVRLVHGIAAALFAALGIATLLGIGGRFGL"
     gene            9930..11234
                     /gene="urtA"
                     /locus_tag="PKI23_04155"
     CDS             9930..11234
                     /gene="urtA"
                     /locus_tag="PKI23_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HNN36169.1"
                     /translation="MAIKQSNLRSTFSAATLAALLGTGALSVQAEETIKVGVLHSLSG
                     TMAISETTLKDTVLMLIDEQNRKGGLLGKKLEAVVVDPASNWPLFAEKAKELLQKEKV
                     AAVFGCWTSVSRKSVLPVFEEQNGLLFYPVQYEGEESSKNVFYTGAAPNQQAIPAVDY
                     LMNDLKVKRWVLAGTDYVYPRTTNKILEAYLKGKGVKSEDIMINYTPFGHSDWQNIVS
                     DIKKFGSQGKKTAVVSTINGDANVPFYKELGNQGISAEQIPVVAFSVGEEELSGIDTK
                     PLVGHLAAWNYFMSVEDAGNAEFIKKWRAFIKSDKRVTNDPMEAHYIGFNMWVKAVEK
                     AGTTDPAKVGEAIIGVTVPNLSGGYSAMMPNHHITKPVLIGEIQSDGQFQTVWKTSGM
                     VVGDAWSDFLPGSQDVTADWMKPLACGNYNVKTGKCSGQNYK"
     gene            11315..12970
                     /gene="urtB"
                     /locus_tag="PKI23_04160"
     CDS             11315..12970
                     /gene="urtB"
                     /locus_tag="PKI23_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNN36170.1"
                     /translation="MASPLSTLLGGRCTPNRLRWLLAVTLLLLINGLRAEDEFAAAVN
                     RLGEGSFAARTALIEQLSRQNDPRTLALFEALLDGRLQSRSDGKVVIVNGSAEPLSLT
                     DPVDGAALPSVTAGELKKIALNNALRSLLRGKISNLRINSSDHLLRLEAARGLVKNPA
                     AEQLPAIEAAIAREQHQQILSTFKLARAMIMAREGEEPQRLEAIHALRGALEPEVRSL
                     LTSLLETDADNSPKEPSAAIRGAAEAVLRQLETEASFYQTLENLFFGLSLGSVLLLSA
                     VGLAITFGVMGVINMAHGEMLMLGAYTTYVVQQLMPGMIDQSLWVAIPAAFIVTALFG
                     IAIERGVIRFLYGRPLETLLATFGVSLILQQLVRSIFTPLNRSVLTPSWMSGSWQLNG
                     ALSITYNRLFIILFSLMVFVGLILLLKRTRFGLSMRAVTQNRQMAQALGIRSGWVDAL
                     TFGLGSGIAGIGGVALSQLTNVGPNLGQAYIIDSFMVVVFGGVGNLLGTLVAALSLGL
                     VNKFVEPLAGAVLAKILVLLFIILFIQRRPTGLFALKGRAAEE"
     gene            13064..14107
                     /gene="urtC"
                     /locus_tag="PKI23_04165"
     CDS             13064..14107
                     /gene="urtC"
                     /locus_tag="PKI23_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNN36171.1"
                     /translation="MLVPLGNLLLPADSVLHIPTYVVSLLGKYLCYALLALAVDLIWG
                     FCGILSLGHGAFFALGGYAMGMHLMRQIGDRGVYGNPLLPDFMVFLNWKELPWYWHGF
                     DALPFALLMVVLLPATLAFLFGWLAFRSRVTGVYFSIITQALTYALMLAFFLNNLGFG
                     GNNGLTDFKELLGFSLQADGTRNALFVASALALAGGYLLCRFIVQSKLGRVLVAVRDA
                     ERRTRFLGYRVEHYKLFVFVVSAMLAGIAGALFVPQVGIINPGEFAPINSIEIIIWVA
                     VGGRATLFGAVLGALLVNYAKSYFTAAFPEVWLYLLGALFVVSTLFFPRGIIGLLERR
                     KKPDPATTTPPGA"
     gene            14111..14893
                     /gene="urtD"
                     /locus_tag="PKI23_04170"
     CDS             14111..14893
                     /gene="urtD"
                     /locus_tag="PKI23_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNN36172.1"
                     /translation="MSAALTISMHDSAPNRTILYLNGVTVSFDGFKALNNLSLVIEEG
                     ELRAIIGPNGAGKSTMMDVITGLTKPDSGEVIFQQNIDLTRHDEAHIANLGIGRKFQK
                     PSCIGSLTVHDNLDLALRGQRRLFSALLRKRSAQQLQRIDELLELVGLIQQRHQPASA
                     LSHGQKQWLEIGMLLAQDPQLLLVDEPVAGMTPQEVERTAELLTGLAGRHSVVVVEHD
                     MAFVRAIAQQRVTVLHQGSVLAEGPMAQVQSDPRVIEVYLGA"
     gene            14895..15590
                     /gene="urtE"
                     /locus_tag="PKI23_04175"
     CDS             14895..15590
                     /gene="urtE"
                     /locus_tag="PKI23_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNN36173.1"
                     /translation="MLEVRGVNQFYDQSHILWDLDLTVEEGLCTCLMGRNGVGKTTLL
                     KAIMGLLPIRSGSIRFMGEELCGKPVEYRAQAGIGYVPQGRMIFPQLTVKENLLLPLN
                     GRKGGPRTIPEQIFSMFPVLQQMLHRRGGDLSGGQQQQLAIGRALALEPRLLILDEPA
                     EGIQPNIVTLIGDVIRELNRHQGISVLLVEQKLAFARNTADRFCIIERGANVDPKITD
                     QLSDELVRRHLAV"
     gene            15670..16458
                     /locus_tag="PKI23_04180"
     CDS             15670..16458
                     /locus_tag="PKI23_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN36174.1"
                     /translation="MDLRLDGKVVMVAAASRGLGEAIARQAAAEGARVAIASSNREAI
                     EAAAQLLRDSTGAEVLASTVDLRQPAQITAWAAEVEQRFGRVDGLVVNAGGPPPGGFD
                     QFDDAAWQQAFELTLMSTVRLIRAVRPLLARQGGAIVAITSSAVKEPIDALLLSNVMR
                     AGVVGLLKSLSRTLAPERIRVNNLIPGSIHTDRIRALNSQMAQLQGVTAEQIEQAATR
                     QIPLGRYGTPDEFARAALFLLCDAASYITGASLQVDGGTLRTLW"
     gene            16493..16753
                     /locus_tag="PKI23_04185"
     CDS             16493..16753
                     /locus_tag="PKI23_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNN36175.1"
                     /translation="MIDRHLFDEFSHALERLLPDTFAPLKRELVESIGLLVEGLVSRL
                     DLVSRAEFDAQSAVLARSRVLLTELEARIAVLERQLAEPPQS"
     gene            16760..18271
                     /locus_tag="PKI23_04190"
     CDS             16760..18271
                     /locus_tag="PKI23_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNN36176.1"
                     /translation="MSLSTILTRARLGIEAPLVQVETHLSNGLPSLAIVGLPEAAVKE
                     SKDRVRSALLNAGFEFPARRITINLAPADLPKEGGRFDLAIALGILTASGQLPAAALA
                     QCEFIGELSLSGGLRPVSGVLPASLAASRAGRALFVPQENAIEAALPQAAEVYAAAHL
                     LEVCAHLRGITPLPASARTAFHATRQSPDLADVRGQHQARRALEVAAAGGHNLLMVGP
                     PGTGKTMLASRLGGILPALSEAEALEVTAIASVATGDRAPISHWPERPFRAPHHTASA
                     VALVGGGSVPQPGEISLAHQGVLFLDELPEFPRKVLEVLREPLESGEIVISRANQRVS
                     FPARFQLVAAMNPCPCGYAGDRSGRCRCTPDQIHRYRDRLSGPLLDRIDLHLTVPALP
                     VRDLLTELPGGESSASVRERVSAARLLQLQRTGKPNRDLTPRELQRHAALAPAEQARL
                     ASALERLGLSARAYHRILRVARTIADLAGSETILTEHLSEAISYRLLDRGSSG"
     gene            18368..19999
                     /locus_tag="PKI23_04195"
     CDS             18368..19999
                     /locus_tag="PKI23_04195"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNN36177.1"
                     /translation="MTCRPPLSLLCALLLCLSRFAGAEPIHLQLRWHHQFQFAGYYAA
                     IEQGYYREAGLDVVIHPGSPGVTPVAEVLTGRAHYGVANSELLLQRLRGQPLVALASI
                     FQHSSSILLSRKERCATTPHDLIGRKVMLMDRQVDADFLAMFKNEGIADSAIELLPSS
                     YQISDLAEGKVDAFNAYRTNEPFYLTQQGIGYCVLDPRNYGVDFYSDILFTSEEELRQ
                     HPERVRAFTAASLKGWRYAMQHPEEIITLLRHKYDVPKSAEHLRYEAETMRSLILPEV
                     IEIGHMNPGRWEHMADTFVRVGMVRDKRWLDGFLHQPDPQAELAQSRRRLWLASGTAV
                     LLGLIALMLYLINRKIRREVALRGQVEAELQRMAYSDALTGLPNRLQFFERADQLLKR
                     ERRDQGRLALCFIDLNDFKKINDQHGHLAGDAVLSQIGQRLRRAVRESDIVARFGGDE
                     FVVLFDNLDTPADLPRLVDHLQSAIRLPIDHQGVTLHSGASIGTALFPDDGDQIDALL
                     SHADQAMYRRKPAARSSPKGVVSPLLAMPNPAPAE"
     gene            complement(20054..21607)
                     /locus_tag="PKI23_04200"
     CDS             complement(20054..21607)
                     /locus_tag="PKI23_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN36178.1"
                     /translation="MYPSIHAARFPDKPAIIMANTGQVITYRELNERSIQVSRLLRSA
                     GLKVGDHVAMFLENHPWFHIITFGAHRAGLYFTPISSRLTASEVDYIAADCMATAFIT
                     SMAKADVAAELVPLMPAVRNRYMLDGVLAGYQSFEEAVLAQSTEPLADETAGVEMLYS
                     SGTTGRPKGILKPLPGTDVTVPNMMMAMLPSRYGFDENSIYLSPAPLYHAAPLGYTRT
                     LLCCGGTIVVMEKFDAEQALQLIEKYRVTCSQWVPTMFVRMLKLPEEDRKKYDLSSHR
                     CAIHAAAPCPVAVKEQMMEWWGPIIYEYYGGSEGNGSTAIEPLEWLQHKGSVGRTAGG
                     AIHICDDEGNELPAGESGTVYFAGMGGPLFQYHNDPKKTAESRHPIHTDWSTLGDVGY
                     LDQEGYLYLTDRKAFMIISGGVNIYPQETENLLVMHPKVADCAVFGVPNEEFGEEVKA
                     VVQLMDKNDASPELAQALIQFCRDNISHIKCPRSIDFIDELPRHPTGKLYKRLLRDRY
                     WGNRDSKIV"
     gene            complement(21769..23895)
                     /locus_tag="PKI23_04205"
     CDS             complement(21769..23895)
                     /locus_tag="PKI23_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN36179.1"
                     /translation="MAPPKNPLSPVRTALKAVDAKEYERLQLALLASRDGIWDWCIQS
                     DQVYYSPRWLEILGYQPDEFPPHLHEWQQRLHPRDADQVLQAMQAHIAGTQPHYQISY
                     RLRARDGRYRWVLARGQVLYDMHDVPVRMVGTITDITGYKQMQRRLQVALEVLRQFSD
                     NLIVIDQNFLVVATNSAFQRNYGYAQETLLGHSPAELLAEPLEAAAAEQLRSDLATQG
                     QWAGELWTRTQAGERILQWVKISAIHVEAQAAPTLHAIVFSPLSNQPRIQERLQRLAY
                     YDHLTGLPNRELFHDRLTVALRQAQRHGEQVALLFFDLNRFKNINDTLGHTMGDLLLQ
                     QVAQRLERVVRVSDTVARLGGDEFTMILTGLHQPNDLQRVMDEVLGQFRQPFRLGEHE
                     LFVTTSVGVSLFPRDGEDEESLIKNADIAMYRAKEQGSNSYQIYSPEFGTRFRQRLTL
                     EADLRKALERNELLLHYQPKIDVNSRRITGIEALLRWQHPTRGLVPPDQFIPIAEETG
                     LILPIGDWVLRKGMEDALPWVRQSSHPFTLAVNLSPVQLQQPDLVQRVARMLDDSGFN
                     PRHLELELTENLLMTNPKASLETLQQLTATGVRIAIDDFGTGYSSLNYLSRFPIDTLK
                     IDKSFIHDLLDDFNNAEIVSTIVAMGHNLNMTVVAEGVESEAQLAYLRDIGCDEAQGF
                     LFSRPIDRVSMELLLVQQADLRQPML"
     gene            24184..25029
                     /gene="traT"
                     /locus_tag="PKI23_04210"
     CDS             24184..25029
                     /gene="traT"
                     /locus_tag="PKI23_04210"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNN36180.1"
                     /translation="MACLLLITLVVAGCGQNVKKEDPSGLSVNGIDLQVKMSANLFIP
                     PPLPPVAESQRRIYLIGTNTSTAQAANERLQPLLIEQLLRRGYLITDNPAEARYMLLY
                     NVLYVGKEVRDLSTITAVSAGVGGAAVAATIGRNNETVQNAGIGGLVGTVTGAVTGHY
                     FETNTFEQVVDLQIRERQPAVPAPLPPEETKRLAVERAIRQVNLALGLGELHTETTPE
                     TDQRLPPSSDNWQLYSSQVVGFATRLRLQFEEAEPLLFQAMAREISGLFWDLHQLPGN
                     TAPRN"
     gene            complement(25044..26186)
                     /locus_tag="PKI23_04215"
     CDS             complement(25044..26186)
                     /locus_tag="PKI23_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN36181.1"
                     /translation="MATLPAEPPPDPTQQAEQGDIQAQWLLAERLAAQNLWQEAMHWY
                     AAAAGQGSASAQLQLGNGYLTGRGVPRDAVQAVQWYRRAAEQGLAAAQVRLGEAALSG
                     IGMAQDQAQAVQWFSKAAQQGDAVGQLRLALAHDSGQGVPRDPRLAAEWFAKAAEQGH
                     PVAQSALGEAYLRGEGVPRDLLQSAQWFAEAAAQGQSQAQFRLGQAYAQGLGIARDPA
                     QAVRWWRQAAEADHPEAAVALGQAYAQGVGTAVDHGSAVHWYTKAALQGDPQAQRLLG
                     LAYYSGEGVPQSYGDAMSWCSRAAEQGDLHAQILLGQFYYRGLGVPQNFEQAYIWVGV
                     AASRFSGAGRQEVLQLRDALAQLLNQEQLLRAQSRALELVRSHHWR"
     gene            26541..28043
                     /locus_tag="PKI23_04220"
     CDS             26541..28043
                     /locus_tag="PKI23_04220"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNN36182.1"
                     /translation="MTAAPALSNVETQPATHVDVLIVGAGISGIDAAYHLRTQCPEKR
                     FVLLETQESFGGTWHTHTYPGIRSDSDLYTFGFKWKPWVGAPIATAEEILKYLKETLD
                     ENDLHQHIRYRHKVKSASWNSQKQQWAVTVEQLDSGELRLFTCNFLWMCQGYYRHEQG
                     YTPDWPGMERFKGVIVHPQSWPADLDYKGKRVIVIGSGATAATIVPAMAAECAHITML
                     QRSPTYFTPGANRNELADLLRELEIPPEWTHEIVRRKVLKDSGQIARLAFEKPEELKA
                     QLIGVVRQQLGDEYDVETHFTPKYQPWRQRLAFVPNGDLFQGIRSGKASVVTDQIETF
                     TEKGLLLKSGQELEADIIVTATGFDLCLLGDVQFTVDGKPVNFADTVTYRGCMFSGVP
                     NMAWIFGYFRASWTLRADLVCELVCRMFNLMQQKGARVVTPQLRDEDRTMARLPWIDP
                     ENFNPGYLARGIHLMPKQGDHEPWVFTQDYHMEKSEFPAANLEDGALVYS"
BASE COUNT         4903 a         9274 c         9025 g         5000 t
ORIGIN      
        1 gccgcgtggt agagcggccc ggtgcagagg tcgagcgaca cacccggctg gtagtcggca
       61 tgcagcagca tccccaccaa ctggtggttg ggtggtgcat tgggtggccg ctgcaccccc
      121 ttgggatggc cggtggtgcc ggaggtgtag agcatcatgc tgccggcgac ggcatcgtcc
      181 ggatcggccg tcgattcgcc ctcgagcgcg gcgtcgtagc ggtcgaagcc ggcgatgtcg
      241 ccacccaccg ccagcccgat cagggcttgg ttgcagctcg ccttcacctg ctgcaacgct
      301 tcggtgaagc gggcatcgcc aatcagcacc cgggcctcgc agtcatcgac gatgtagctg
      361 atttcgcgcg gggtcaggtg ccagttgacc ggcgtcaccc gcaggccgcc gcgcatgcag
      421 gcgatcaggg tctcggggaa ctccggccgg ttggcgcaga gcagtgccac cgcatcgccc
      481 gggcgcagcc cgcgccggcg cagtgcctgc agcagctggt tcgcgctggc gttgagtgca
      541 tcgaaggtgc gaacaccatg ctcggactgg atcgccaacc gctgcggcgt ctgccgggca
      601 tggatcgcga gtcccatgcc gcactgggca gcctgttccg ggctgagctg ggtgtgttgt
      661 atcgtctgct tcatggccac tctccttgat ccttgttttg attggatttc tcgaacagcg
      721 tactcctgtc acgcagtcat cataggaggc cgtcagcgcc acaacaatcg gtcgacactt
      781 gagtcatgcc ggtgcagcct gcgccgctta tccaggaaaa tggcttgtgt tatggtgaaa
      841 cagtgcacat ttttcggcgg ccgttctggc tagcatggcc tcaatcactc caccggatgg
      901 tgagtatctg ccgaaaacga cggtgtgcag catgttcatg gtacggaagg agccactatg
      961 agtgtcgatt cagtctcacc ctggcagcag gtccgcgcat tgcagactgc gctgttcaac
     1021 ggggtgcaac cacagaaact gcttgccgaa gcgttgcagc tgctggtcga gcagatgcag
     1081 gtgcgcatcg gcacgctgct gcgggttgag cgtcagcacg atggccttta tcgactggag
     1141 ccgctctgtt gccatcaaca gggtcagaac ggtcccgacc gagcgctgcc tccgttcctg
     1201 tcactgaatg gcgatgagca gccgctcctg cagcggatgc tgatggccga aacagtgcag
     1261 cgctggctgg ccgccgactg cccggtttcg ctgacggcaa aattgggcca cgccgacccg
     1321 tgtgacagcg tgagcaccct cacgctgcat catggcggag agccgatcgc gctgctgtgg
     1381 ctggtcgacc cttcggccga tccactgccc gctgacggca gcgcgctcga gctgctgcga
     1441 ctgaccttga tccaccttct gctcgatctc aaccggcgtc gccagcagca gcggcgccac
     1501 aaactggccg agggctgcac cggggccttg acggcactgg cgcaacaacg gccgatcgag
     1561 gagattctga cgctgttggc cgacaccctg gtgcgactgt tccccgatcg tggctgtctg
     1621 ttcatgcggc tcaaccgtaa gcgagaccgg ctcgaaccgg tggcgtgggc agggctgagc
     1681 gccgcccatg tcgctgcggt gcgcgacttt ccggtcgatg cccgatcgat ctgttgcggg
     1741 ctggctgcct ggcgcggcga agaggtgatg gtcgattcgc tcgaagaagt gctgaccgat
     1801 gcaccgctgc gcggtgtgat catcggcagt ggctttcagg cctgctggag tcggccactg
     1861 ctcgatgccg cgcaacgggt ggccggcaca ctggcgatct atggccgttc acccttgcat
     1921 ctgagtgagg gcgatcggcg catgatcgag caggtcacgc aggtggcggc cctgaccctg
     1981 agccactgga ccgcccttga actgctgcgc gagcgcgacc agatgctggc tggcgctttc
     2041 gaccgcgcgg tccacccgat ggcgctgtgc gacacccagg ccaacttcct gcaggtcaac
     2101 gatgccttct gtgactggct tggctatccg cgcgaacaac tgttgcggat gagtgttttc
     2161 gacatcgttt ttcccgatgc actctcctcg gcacgcgaga ttttgcagcg cggcagcgcg
     2221 gagcgtgcgt tccgcgccga tgagcggcgc taccgccacc gtgatggctc ggtacgccat
     2281 gccgcggtca gcatctcggt ggtggttgac cagcacggca ccttcagctc ggcagtgctc
     2341 gatttcttcg atctgacacc gcaccggcag atgctcgaca ccctgcggca gcgcgaagcc
     2401 gaactgctgg cctcgcagcg gctggcccgg ctgggctcct ggcggctgga ggtggcgagc
     2461 ggtcacctca gctacaacca ggaggccatg aagattctag gcagcgatcc acgggtgacc
     2521 gaggccttga ctacattgag tggtttccag cagctggtgc atcccgccga ccggtcacgg
     2581 ctcgcagagg ccctcgaggc gacgctgcgt gatcttcagc cctatcagat cgaataccgc
     2641 tttctgctgc cggagggccg gttgttgtgg gtggattcaa gaggagaggt cgaactcgct
     2701 gccgacggcc agttggtggc ggtctccggg gtgtttcagg atgtgaccga acgcaagcgg
     2761 gtcgaggagt cgttgcgggc cagcgaagcc cgctttcgcg gcaccttcga aagcgcagcc
     2821 catggcatgg ccatcaccag cccgaccggt cacttcatcc aggtcaacca gtcgctggcg
     2881 cgcattctcg gccgcgcgcc cgaggcgctg atcggccggc cggtggccga tttcgctccg
     2941 ccacaggatc tggagcggga tcgggagatc atccggcagt tcagcagcgc gaagctgcgc
     3001 cagctcacct tcgacaagcg ctatctgcgg ccggatggcc atctggtgtg ggccaccctc
     3061 agtgtggcgg gcgtctatga ccagaacggc gcattgcagc atttcatcac ccacacgctc
     3121 gatgtgaccg aacgcaagca ggccgaggcg gcgctcgatg aagagcggga gcggctcttc
     3181 gtcaccctca actcgatcgg cgacgcggtg atcaccaccg atgccgaggg ccgcgtcacc
     3241 tttctcaacc cggtggccga gcagctcacc ggctggtaca acaccgaggt gctgggccgt
     3301 ccgctcgaac aggtcttcca catcatcagc gaagatcatc ggcaacaggt ggagagcccg
     3361 gtacagcggg tgctgctcga agggcgcatc gtcgggctgg ccaaccacac gctgctgatc
     3421 aaccgcctcg gccaggagat cgccatcgag gattcggccg cgccgattcg caacctcgcg
     3481 ggcgacatca tcggcgtggt gctggtcttt catgacgtca gccaggcgcg acagatggcg
     3541 gtcgagatga gctggcaggc gacccatgac gccctgaccg ggctggtcaa ccgccgcgaa
     3601 ttcgagcacc gcttgagcga ggcgatcaag cgctgctggc aggagcaggt cactcacatc
     3661 tttctctacc tcgacctcga ccagttcaag ctggtcaacg acacctgcgg ccatctggcc
     3721 ggtgacgaac tgctgcggca actgacccat ctgctgcacg ggcgcatccg cgacatcgac
     3781 accctgtcac ggctgggtgg cgacgagttc ggcgtgctgc tggagcactg ctcgctggag
     3841 aaggggctgg agattgccga cacgctgcgc acgctggtgc gcgaattccg cttcgtctgg
     3901 gaagagaaga tcttcgaggt gggggtcagc atcggcctgg tgttgatcga cgaacagatc
     3961 gagagcgccg acaaggcact gagtctggcc gatctggcct gctatgccgc caaggatctc
     4021 ggccgcaacc gggtccatgt ctatcaggcc gatgatcacg aactggcgcg ccgccatggc
     4081 gagatgcagt gggcggcacg catcagccgg gcactggccg aaaaccgttt cacgctctac
     4141 cggcagcaga ttcgaccggt gcgggacgag cagcgcgaac cgctccattt cgagctgctg
     4201 atccgcatgc tgggcgagca ggatgagctg atcctgccct cgcagttcat cccggccgcc
     4261 gagcgctaca acctgatgcc gaacatcgac cgctgggtcg tcgagcactg cttccgctac
     4321 ctgtcacgcc accatgatgg ccagacccgc tttaccatca acctgtcggg aatgagcctt
     4381 ggtgaagagg ccaccctcga cttcatccgg gagcggatgc gccactacca gatcgatccg
     4441 ctctgcctct gcttcgagat caccgaaacc gccgccatcg ccaacctggt caaggcgtcg
     4501 cgcttcatca actcgctgaa gtcgctgggc tgccgcttcg cgctggatga cttcggcagc
     4561 ggtctctcct cgttcgctta cctgaagaac ctgccgatcg actacctgaa gatcgatggc
     4621 agcttcgtca aggacatggt cgacgacccg atcgaccggg cgatggtcag cgccattcac
     4681 cagatcggcc aggcgatggg catccgcacc attgccgaat tcgtcgagaa cggggcgatt
     4741 ctggaacagt tgcgcgccat cggcctcgac tacgcgcagg gctatgcgat cgcctggccc
     4801 gaaccgctgc ccaacttctg aatccagccg gaatcaggcg aaagaccgaa ctctcgggca
     4861 caaaaaaggg tctgcctcac cggaggcaga ccctctggtc tgatcgatct cagccgcggc
     4921 gacgcaccag cagcagcgtc agcgccgaac cgaacagcca gacaccggcc ggcagcggca
     4981 cggcggtgag ctgggcatac atccggtcgg ccagtatctg ctgggcggcg gtggtggggt
     5041 gtttgtcatc gaagaacagg tagccagcgc aacccgccgg caggccaatg cagccgctcg
     5101 tggcattggc aaaagtaggc gccaactgtt gcagggttga gaaggtgtcg aacagctcga
     5161 tgtcggcctg gaggctggcc agcccgctgc tcaactggct gttgaaccac tggctgtact
     5221 gggtggcatc ggcattgccc agctcagcgg gagtcatgcc cagatcgggg atgtttggca
     5281 ccagcagatg ctgggcgcca tgggccacca gatcgcccac cacgctcacc agcaggctgg
     5341 ccaccccttc gaccgcagcc tgtgcatcgt gaggcgtcaa tgactggcca agcagaccga
     5401 acaggtcatt gttgccgccc cagaccacat agagcgcatt ggagtcgagg ctgccgccgg
     5461 tatcggtgaa cagcgcggtg cgctgcgaag agagtgaggg aatgatgccc agcgccgcag
     5521 cagagaccag cgcaccgccg tacgcataat cagtgccgcc ttggcgcgac ggggtgacgg
     5581 cgcccagacc cagactgctg ctcagatatt caacatagac cgggccattg gagaagcggc
     5641 cgttggagta gggtggcggc ggtgtggctg gaatgccgaa gattggtgga agggaggcag
     5701 tgaggtcgta gaggttgccg gtgtcgctga ggctgtcgcc aatcaccacc agttggctgt
     5761 aggaggcggc gaacagcggg gtggaaccga gcagagccat catcagcacg atcgagcggc
     5821 agcggcgcaa catggtcatc tccctgattg agttgtcgga acggaccagc ggacgatacg
     5881 tcaacccgat ggctctgtaa atcgcgctgc ggtcacgatc gccgctggta acggcccggc
     5941 gccacctcga tgacccgctc ctgctccagc agccggtcga ggtgctggcg cagcatgttg
     6001 cgttcgttgc ggtcggtgtg gtggccggtg tcggccgggc ggtagacgat gcggtgggcc
     6061 actgcctctt cgagcgtgtg cggctcgctc aggtagtcga gcagcctggc ttcgcgctgc
     6121 gcgatcatgc catggtactg ggcagcgcgc gctctgaacg cgggctgatc gagcacgccg
     6181 atgtggtggt aggtgacgaa ccaggccaga tcgagctggc gcacttcggt caggctgcgg
     6241 tcgaagtcga ccaggctgga gttggggccg ccatagtagg ggccgaagcc ggtcagctcg
     6301 atgtcggcca gaaagatcag ctcatggcgg cgctgctcgc cacgcacgat gaaacaggtg
     6361 tggccatggg tgtggcccgg ggtgtgcaga aactgcacct tgacccggcc gaggtccagc
     6421 tcgcgctcga cctcgaaggc ggtggcatcc ggacgcggcg tgtagttgaa gtagtcgcgc
     6481 acattctggc tgaactgttc ccgctcctcg tcgaccacgc catgcagttc cagcaatccg
     6541 tcgatgccct gaatggccgg cagctccagc ggatgggcct ggcagaggct gtcggggaac
     6601 agatgcaccc cggcgacatg gtcctggtgg cagtggctga gcagcacgcg gtcgacgcca
     6661 gccagatcga cgctgtggta cttgaggctc ttggaggggt cgatgatcag cgactcttcg
     6721 ctgccgcgca ccagaatcga attgccatag gggaacttgc caccattgcg ccccatcagc
     6781 acctgggcac gcccgaactc cagaaccggt ggtggttcga cacgactgct ggccaatccc
     6841 atggcgactc ctcatgacaa cggtacaaaa aacggccggc tttatatcac atcgcgaggc
     6901 gcccgtcttc agcgaccggc cttgcattcg cccggtggcg ggataatatc ggaccattgc
     6961 catcaggcat tgctgcaccg actccatcga tctacccctt gcgaggtgac ccagatgtcc
     7021 gaccaggcga ttgtttcgac ccagcgcgcc caggcgctga ctgccagcag caaggtgctg
     7081 cgcaacacct atgcgctgct cgcgctgaca ctgctgttca gcgcattgac tgccgcgctg
     7141 tcgatggcgg tcaacgcccc ctatttcggt cttttgagcc tgctgcccta cttcggcctg
     7201 ctctacgcca ccgagaagag caagaacagc ggctgggggc tggtgtgggt ctttgcgctg
     7261 accggctggc tcggcttcac gctggggccg attctcaacc actatgtcgg tctgcgcggg
     7321 tatgaaccgg tgctgctggc gctcggcggc acggcggcga tctttctgtc gctgtcggcc
     7381 tatgtgctgg tgacccgcaa ggagctgagc ttcatgaccg gctttctggc gatggggatg
     7441 ctggtggcct tcatcgccgc gatcgccaat gcctttctgc agatccaggc gctgacgctg
     7501 gccgtcagct gcatgttcct gctgctctcc tcggcgctga tcatgtggca gaccagcgcc
     7561 atcatccatg gcggtgaaac caactacatc tccgccaccg tcgggctcta tgtggcgatc
     7621 tacaacctct tcaccctcct gctgggcttt ctgggacagg atgactgacg cttgcccgct
     7681 cccccgcaga ccccgcccac cggcggggtt tcgttttgcg atgaggccct gatgcgtttc
     7741 gcgatcgcca tccatggcgc ccccttcagc caccaggggg cgcagagcgc ctaccgcttc
     7801 gcgctggcga cgctggccgc gggacatcgg attggccgca tctttttcta tcaggatggg
     7861 gtgcacaacg gcaatcggct gatcacgccg gcccaagatg agctcaacct gcccgactgc
     7921 tggcaaacgc tggctgaaca gcatgagatc gaactggtgg tctgcatcgc cgcggcggcc
     7981 cggcgcgggg ttctggatca gcgcgaggcc gcccgtcacc agcatccagc cgccaacctg
     8041 gcgcccggtt tcacgctctc cggtctcggt cagtggatcg atgcgctggc cgagagcgac
     8101 cgtttcatcg ccttcggccc ctgaagcgag cagaatccat gaagctcgcc atcgtgcaga
     8161 cccgcccgcc gcatcagcag gccagcggcc gcgaagggct cgacaccgcg ctggtcggcg
     8221 cggtgttcga cctcgaggtg agcctgctgt tcgtcgacga tggcgtctat caactgctgg
     8281 ccggccagga tcctgccgcc atcggccaga agccgtggtg tgcggccttc gaagccgctg
     8341 aactctatgg catccggcaa ctgctggtcg atgccgaggc gctggccgag cgcggcatcg
     8401 aagtcggcga gctgatggtg ccggtcgaac tgcgatcggg cagcgaactg gcgcaacacc
     8461 tcgacagctg cgaccaggtg ctggtcttct gagcgccatg cccagcctgc acaccctcaa
     8521 caaatccccc accgctgcgc cactgctgcg ccgctgcctg cggctggcac agccgggcag
     8581 caccatcgtg ctgatcgagg atgcggtcta tgccgccgtc gatggctcgc cgggcgccgc
     8641 gctgctgcgt gcccaccatg accgcttcac cttccatgcg ctgcaagccg acctgcaggc
     8701 acgcgggctg gtcggccggc ggctggtcga ggcggtcggc gtgatcgacg atgccgactt
     8761 cgtcgcgctg gtggtgcaac acccacggat catcagctgg tactgagcgc ggcggttgcg
     8821 ccaaaaccca tcacaggccg aaacggccgc cgatgcccag cagggtggcg atgcccagcg
     8881 ccgcgaacag cgccgccgcg atgccatgca ccagcctgac cggcagccgt tgcgcgatgc
     8941 gctcgcccag cagcactgcc ggcacgttgg ccagcatcat ccccagcgtg gtgcccagca
     9001 ccaccgcgat caggctctgg tattgggccg ccagcgccac ggtggcgatc tgggtcttgt
     9061 cgcccatctc ggccataaag aaagtaagca gcgtggtgcc gaacacgccg aaacgactgc
     9121 tggccacctc gccctcgtcg aaccggtccg gcaccagcac ccaggcggcc atcgccagga
     9181 atgcgccacc cagcagccag cgcaggctct gcggcgagag ttgagcggtg atccagctgc
     9241 caagcgcgcc ggccagacca tggttgaaca gcgtggccag cagaatgccg agcacgatcg
     9301 gccagggtcg gcgaaagcgc gccgccagaa tgaaggcgag caactgggtc ttgtcgccga
     9361 tttccgccag ggcgacgaca ccggtcgaga tgagaaaggc ttgcacgcgg cggctccgtg
     9421 aatcgtgaca tcgatggcat cccgctgcag gaggctgcgg cggggcgcac cattctggca
     9481 cacggacaaa aaaccgccaa aagcgcggct gtaccgcgct gcatcgactc ttcatccctg
     9541 atcgcatcgg gtgcgtggca cgccgttcgt gcacccgact ggcgcatcag ctcgccctgt
     9601 tgtgtgcgca tctgattcga aggccgctca ccgcccctgt gaaccagccc tccgtgacac
     9661 tggacgccgg caatcgatcg aatcggtgcg gacaagggca acttctgctc tgtttcagcg
     9721 caatgtgccg ccctcactgc atcgactttc cgaaccgggt cgacaatttc agcaagcgcc
     9781 gaacattggc atcaaccctg cttaatgtat gcaactcgcc ttgcgagcag ccctgccaca
     9841 ccagcgcggg ccatgcagca agagggggtt caggaatggc agttcgacac aaaacggcac
     9901 aatcgcacga cacatcaagg agctacttca tggccatcaa acagagcaat ctgcggagca
     9961 ctttcagtgc cgccacgctc gccgcactgc tgggcaccgg cgccctgtcg gtgcaggcgg
    10021 aggagaccat caaggtcgga gtgctgcact cgctctccgg caccatggcg atcagcgaga
    10081 ccacgctgaa ggatacggtg ctgatgctga tcgacgagca gaacaggaag ggcggattgc
    10141 tcggcaagaa gctggaggcg gtggtggtcg atccggcttc caactggccg ctgttcgccg
    10201 agaaggccaa ggagctgttg cagaaggaga aggtcgcggc ggtctttggc tgctggacct
    10261 cggtgtcgcg caagtcggtg ctgccggtgt tcgaggagca gaacggcttg ctgttctatc
    10321 cggtgcaata cgagggtgag gagtcgtcga agaatgtctt ctacaccggt gccgccccca
    10381 accagcaggc gattccggca gtcgactacc tgatgaacga cctgaaggtg aagcgctggg
    10441 tgctggccgg caccgactat gtctatccgc gcaccaccaa caagattctc gaagcctacc
    10501 tgaagggcaa gggggtcaag agcgaagaca tcatgatcaa ctacacgcca ttcggccact
    10561 ccgactggca gaacatcgtg tcggacatca agaagtttgg ctcgcagggc aagaagaccg
    10621 ccgtggtctc caccatcaat ggcgatgcca acgtgccctt ctacaaggag ctgggtaacc
    10681 agggcatctc ggccgagcag attccggtgg tggccttctc ggtgggtgag gaggagctct
    10741 ccggcatcga caccaagccg ctggtcggcc atctcgccgc ctggaactac ttcatgagcg
    10801 tcgaggatgc cggcaatgcc gagttcatca agaagtggcg cgccttcatc aagagtgaca
    10861 agcgggtcac caacgacccg atggaagcgc actacatcgg cttcaacatg tgggtgaagg
    10921 cggtcgaaaa ggccggcacc accgacccgg ccaaggtcgg cgaggcgatc atcggcgtga
    10981 ccgtgcccaa cctctcgggt ggctactcgg cgatgatgcc caaccatcac atcaccaagc
    11041 cggtgctgat cggcgagatc cagtccgatg gccagttcca gaccgtctgg aagacctccg
    11101 gcatggtggt gggtgatgcc tggtccgact tcctgcccgg cagccaggat gtcaccgccg
    11161 actggatgaa gccgctggcc tgcggcaact acaacgtcaa gaccggcaag tgctcgggtc
    11221 agaactacaa gtgacccgca cagcgggtgc ccatcgaacg cggtgggcat ccggtgcgtt
    11281 gcagcatgtc ttgagtgacc cacggacttc gaccatggca agccctctct caacgcttct
    11341 tggcggccga tgcacgccca atcggctgcg gtggctgctc gctgtcacgc tgctgctgct
    11401 gatcaacggc ctgcgcgccg aggatgagtt cgccgctgcc gtcaatcggt tgggggaggg
    11461 ttcgttcgct gccagaaccg ccctgatcga gcagctttca cggcagaatg acccgcgcac
    11521 gctggcgctg ttcgaggcgc tgctcgacgg ccggctgcag agccgcagtg acggcaaggt
    11581 ggtgatcgtc aacggcagtg cggagccgct gtcgctgacc gatccggtcg atggcgcggc
    11641 cctgccatcg gtgactgccg gcgaactgaa aaagatcgcc ctcaacaatg cgctgcgcag
    11701 cctgctgcgc ggcaagatct ccaatctgcg catcaactcc agcgaccatc tgctgcggct
    11761 ggaggcggcc cggggcctgg tcaagaaccc agccgccgag cagttgccgg cgatcgaggc
    11821 cgccatcgcc cgtgagcagc accagcagat tctctccacc ttcaaactgg cgcgggcgat
    11881 gatcatggcg cgcgagggcg aggagccgca gcgactggag gcaatccacg cgctgcgggg
    11941 cgcgctcgaa cccgaggtgc gcagcctgct gaccagcctg ctcgaaaccg acgccgacaa
    12001 cagtccgaag gagccctcgg cggccatccg cggtgcggcc gaggcggtgc tgcggcagct
    12061 cgaaaccgag gccagcttct atcagaccct cgagaacctc ttcttcggcc tcagcctcgg
    12121 ctcggtgctg ctgctgtcgg cggtgggact ggcgatcacc ttcggcgtga tgggtgtcat
    12181 caacatggcc catggcgaga tgctgatgct gggggcctac accacctacg tggtgcagca
    12241 actgatgccg ggcatgatcg atcagtcgct gtgggtggcg attccggccg ccttcatcgt
    12301 caccgcgctg ttcggcatcg ccatcgagcg cggggtgatc cgctttctct acggccgacc
    12361 gctcgaaacc ctgctcgcca ccttcggcgt cagtctgatc ctgcagcagt tggtgcggtc
    12421 gatcttcacc ccgctcaacc gctcggtgct gacaccgagc tggatgagcg gctcctggca
    12481 gctcaatggt gcactgtcga tcacctacaa ccggctcttc atcattctct tcagcctgat
    12541 ggtctttgtc ggactgatcc tgctgctcaa gcgcacccgc ttcggcctca gcatgcgcgc
    12601 ggtgacccag aaccggcaga tggcgcaggc gctcggcatc cgctccggct gggtcgatgc
    12661 gctcaccttc ggtctcggct cgggcatcgc cggcatcggc ggcgtggccc tcagccagct
    12721 gaccaacgtc ggccccaacc tcggtcaggc ctacatcatc gactcgttca tggtggtggt
    12781 gttcggcggc gtcggcaatc tgctcggcac cctggtcgcc gcactgtcgc tggggctggt
    12841 caacaagttc gtcgaaccac ttgccggtgc ggtgctggcc aagattctgg tgctgctgtt
    12901 catcatcctc ttcatccagc ggcgcccgac cgggctgttc gcgttgaagg ggcgtgccgc
    12961 cgaggagtga tgcatgaccc gtcgatccac ttcgctgctg ccagcgctgc tcgccaacga
    13021 ccgcggcgga cagctgctgc tgggattgct gctgctggcc gccatgctgg tgccgctcgg
    13081 caacctgctg ctgccggccg actccgtgct gcacatcccc acctatgtgg tcagcctgct
    13141 gggcaagtac ctctgctatg cgctgctggc gctggcggtc gacctgatct ggggcttctg
    13201 cggcattctg agcctcggcc atggcgcctt ctttgcgctg ggcggctatg cgatgggcat
    13261 gcatctgatg cggcagatcg gtgaccgcgg cgtctatggc aatccgctgc tgcccgactt
    13321 catggtcttt ctgaactgga aagagctgcc ctggtactgg catggcttcg atgccttgcc
    13381 gttcgcgctg ctgatggtgg ttctgctgcc ggccaccctg gcctttctgt ttggctggct
    13441 cgcctttcgc tctcgcgtca ccggggtcta cttctcgatc atcacccagg cgctcaccta
    13501 tgcgctgatg ctggccttct ttctgaacaa cctcggtttt ggtggcaaca acggtctgac
    13561 cgacttcaag gagctgctcg gcttcagcct gcaggccgat ggcacccgca acgcgctctt
    13621 cgtcgcctcg gcgctggcgc tggccggcgg ctatctgctg tgccgcttca tcgtgcaatc
    13681 gaaactgggt cgggtgctgg tggcggtgcg cgacgccgag cggcgcaccc gctttctcgg
    13741 ctaccgggtc gagcactaca agctgttcgt cttcgtcgtc tcggccatgc tggccggcat
    13801 cgccggcgcg ctgttcgtgc cacaggtcgg catcatcaat ccgggtgagt tcgcgccgat
    13861 caactcgatc gagatcatca tctgggtcgc cgtcggcggg cgggccacgc tgttcggcgc
    13921 ggtgctgggt gcgctgctgg tgaactacgc caagagctac ttcaccgccg ctttcccgga
    13981 ggtctggctc tatctgctcg gcgcgctctt cgtcgtctcg acgctgttct ttccgcgcgg
    14041 gatcatcggc ctgctcgaac ggcgcaagaa gcccgatccg gccactacca cgccaccggg
    14101 agcctgagcg atgagcgcag ccttgaccat ctcgatgcac gacagcgcac ccaaccgaac
    14161 cattctctat ctgaatggcg tcaccgtcag ttttgatggc ttcaaggcgc tcaacaacct
    14221 ctcgctggtg atcgaggagg gcgagctgcg cgccatcatc ggccccaacg gcgccggcaa
    14281 gtcgaccatg atggatgtga tcaccggact caccaaaccg gacagcggcg aggtgatctt
    14341 tcagcagaac atcgacctga cccggcatga cgaggcgcac atcgccaacc tcggcatcgg
    14401 ccgcaagttc cagaagccga gctgcatcgg cagcctgacc gtccacgaca acctcgatct
    14461 ggcactgcgc ggccagcgcc ggctgttctc ggcgctgctg cgcaagcgca gcgcgcagca
    14521 gttgcaacgc atcgacgagc tgctggagct ggtcgggctc atccagcagc gtcatcagcc
    14581 cgccagcgcc ctctcccatg gccagaagca gtggctggag atcggcatgc tgctggcgca
    14641 ggatccgcaa ctgctgctgg tcgatgaacc ggtggccggc atgacgccgc aggaggtcga
    14701 gcgcaccgcc gaactgctga ctggcctcgc cggccgccat tcagtggtgg tggtcgagca
    14761 tgacatggcc ttcgtgcggg cgattgccca gcagcgggtg accgtgctgc atcagggcag
    14821 cgtgctggcc gaagggccga tggcgcaggt gcagagcgat ccgcgggtga tcgaagtcta
    14881 tctgggggcc tgagatgctc gaggtgcgtg gcgtcaacca gttctacgac cagagccata
    14941 tcctctggga tctcgacctc accgtcgagg aggggctctg tacctgcctg atgggccgca
    15001 acggcgtcgg caagaccacg ctgctgaagg cgatcatggg gctgctgccg atccgctccg
    15061 gttcgatccg cttcatgggc gaggagctgt gcggcaagcc ggtcgaatac cgtgcccagg
    15121 ccggcatcgg ctatgtgccg caggggcgga tgatctttcc gcaactgacc gtcaaggaga
    15181 acctgctgct gccgctcaat ggccgcaagg gtggaccacg gacgattccc gagcagatct
    15241 tctcgatgtt cccggtgctc caacagatgc tgcaccgccg tggtggcgac ctctccggtg
    15301 gtcagcagca gcagttggcg atcggccggg cactggcgct cgaaccccgg ctgctgattc
    15361 tcgacgaacc ggccgagggg attcagccca acatcgtcac gctgatcggc gatgtgatcc
    15421 gcgaactgaa ccgccatcag ggcatcagcg tgctgctggt cgagcagaag ctggccttcg
    15481 cccgcaacac cgccgaccgc ttctgcatca tcgaacgcgg cgccaacgtc gatccgaaga
    15541 tcaccgacca gctcagtgac gaactggtgc gccgccatct ggcggtctga ccgccacgcg
    15601 gttggcacga agcgcggttg atcccgtatc atcctgcaca gtggttcgat cgacagcggg
    15661 agcaggcaca tggatctacg gctcgacggc aaggtggtga tggtggcggc ggccagccgc
    15721 ggtctgggtg aagcgatcgc ccggcaggcg gcggccgaag gggcgagggt cgccatcgcc
    15781 agcagcaacc gcgaggccat cgaggccgct gcgcagctgc tgcgtgacag caccggcgcc
    15841 gaggtgctgg ccagcacggt cgacctgcgc cagccggcgc agatcactgc ctgggcggca
    15901 gaggtcgaac agcggttcgg ccgcgtcgat ggcctggtgg tcaatgccgg cggcccaccg
    15961 cccggcggct tcgaccagtt cgacgacgcc gcctggcagc aggccttcga actgacgctg
    16021 atgagcacgg tgcggctgat ccgtgcggtg cgtccgctgc tggcgcgcca gggtggcgcc
    16081 atcgtcgcca tcacctcgag cgcggtgaag gagccgatcg acgcgctgct gctctccaac
    16141 gtgatgcgtg ccggcgtggt cggcctgctc aagagcctgt cgcgcacgct ggcgcccgaa
    16201 cggattcggg tcaacaacct gattcccggt tcgatccaca ccgaccgcat ccgcgcgctg
    16261 aacagccaga tggcgcagtt gcagggtgtc accgccgagc agatcgaaca ggccgccacc
    16321 cggcagattc cgctcggccg ctacggcact cccgatgaat tcgcccgcgc cgcactcttt
    16381 ctgctctgtg acgccgccag ctacatcacc ggcgccagcc tgcaggtcga tggcggcacc
    16441 ctgcgcaccc tctggtaaca tctgcgctcc cgcagccgat gacgagccag ccatgatcga
    16501 tcgccacctg tttgacgaat tcagccacgc cctggagcgg ctgctgcccg ataccttcgc
    16561 cccgctcaag cgcgaactgg tggagagcat cggcctgttg gtcgaggggc tggtcagccg
    16621 gctcgacctg gtcagccgcg ccgaattcga cgcccagagt gctgtattgg cccgcagccg
    16681 tgtgctgctg accgaactgg aggcgcgcat cgccgtgctc gaacggcaac tggccgagcc
    16741 cccccagagc tgacccgaag tgtcgctctc gaccatcctc acccgggccc gactcgggat
    16801 cgaggcaccg ctggtgcagg tcgagaccca cctctccaat ggattgccga gcctggcgat
    16861 cgtcggcctg cccgaggcgg cggtgaagga gagcaaggac cgggtgcgca gcgcactgct
    16921 caatgccggc ttcgagtttc cggcccggcg catcaccatc aacctcgccc cggccgatct
    16981 gcccaaggag ggtggccgct tcgacttggc catcgcgctg ggcatcctga ccgcctccgg
    17041 ccagttgccg gccgccgcgc tggcgcagtg cgagttcatc ggcgagctgt cgctgagcgg
    17101 cgggctgcgg ccggtcagcg gcgtgttgcc ggccagcctg gccgccagcc gcgccggccg
    17161 cgcgctcttc gtgccgcagg agaacgccat cgaggcagcg ctgccgcagg cggccgaagt
    17221 ctacgcggcc gcccatctgc tggaagtctg cgcccatctg cgcggcatca caccgctgcc
    17281 ggccagcgcg cgcactgcct tccacgccac gcgccagagc ccggatctcg ccgatgtgcg
    17341 cggccagcat caggcgcggc gggcgctcga agtggccgcc gccggcggcc acaacctgct
    17401 gatggtcggc ccgcccggca ccggcaagac catgctggcg agtcgcctcg gcggcatcct
    17461 gcctgcgctc agtgaagccg aggcgctgga ggtgactgcc atcgcctcgg tcgccacggg
    17521 tgaccgcgca ccgatcagcc actggccgga gcggccgttt cgtgccccgc accacaccgc
    17581 gtcggcagtg gcgctggtcg gaggtggcag tgtgccgcag ccgggcgaga tttcgctggc
    17641 ccatcagggg gtgctgtttc tcgatgagct gccggagttc ccgcgcaagg tgctggaggt
    17701 gctgcgcgaa ccgctcgaaa gtggcgaaat cgtcatctcc cgcgccaacc agcgggtcag
    17761 ctttccagcc cgcttccagc tggtggcagc gatgaatccc tgcccctgcg gctatgccgg
    17821 cgaccgctcc ggccgctgcc gctgcacgcc ggatcagatc caccgctacc gtgaccggct
    17881 ctccggcccg ctgctcgacc gcatcgatct gcatctgacc gtcccggcac tgccggtgcg
    17941 cgatctgctg accgagctgc ccggcggcga gtccagcgcc agcgtgcgcg agcgggtcag
    18001 cgccgcccgg ctgctgcagt tgcagcgcac cggcaagccg aaccgcgatc tcaccccgcg
    18061 cgaactgcaa cgccatgccg ccctggcgcc ggccgaacag gcccgactgg ccagcgcgct
    18121 ggagcggctc gggctgtcgg cgcgcgccta ccaccgcatc ctgcgggtgg cacgcaccat
    18181 tgccgatctg gccggcagcg agaccatcct caccgaacac ctctccgagg cgatcagtta
    18241 ccggctgctc gaccgcggca gcagtggttg actgtttcgc ggccagcgca accccccatc
    18301 cacggccttg atcgccaccg gcaaacggtt accattcgac catcgtgcac agagaatgga
    18361 cgccgagatg acctgccgcc ccccactgtc cctcctctgt gcactgctgc tctgtctgag
    18421 ccgattcgcc ggcgccgagc cgattcacct gcaactgcgc tggcatcacc agttccagtt
    18481 cgccggttac tacgccgcca tcgagcaggg ctactaccgc gaggccgggc tcgacgtggt
    18541 gatccacccc ggctcgcccg gtgtgacccc ggtggccgag gtgctgaccg gccgcgccca
    18601 ctatggtgtc gccaacagcg agctgctgct gcaacggctg cgcggtcagc cactggtggc
    18661 gctggcctcg atctttcagc actcgtcgtc gatcctgctg tcgcgcaagg agcgctgcgc
    18721 caccaccccg catgacctga tcggccgcaa ggtgatgctg atggaccggc aggtcgatgc
    18781 cgattttctc gccatgttca agaacgaggg gatcgccgac tccgccatcg agctgctgcc
    18841 cagcagctat cagatcagcg acctggccga gggcaaggtc gacgccttca atgcctaccg
    18901 gaccaatgag cccttctacc tgacccagca gggcatcggc tactgcgtgc tcgaccccag
    18961 gaattacggg gtcgacttct acagcgacat tctcttcacc agcgaagagg agctgcgcca
    19021 gcaccccgag cgggtgcgtg ccttcaccgc cgccagcctg aaggggtggc gctacgcgat
    19081 gcagcatccc gaggagatca tcaccctgct gcgacacaag tacgatgtgc ccaagagcgc
    19141 cgaacacctg cgctacgagg ccgagacgat gcgcagcctg atcctgccgg aggtgatcga
    19201 gatcggtcac atgaacccgg gccgctggga gcacatggcc gacaccttcg tccgggtggg
    19261 catggtccgc gacaagcgct ggctcgatgg ttttctgcac cagcccgatc cccaggccga
    19321 actggcccaa tcgcgtcgcc ggctctggct cgcctccggc actgccgtgc tgctggggct
    19381 gatcgcgctg atgctttatc tcatcaaccg gaaaatccgc cgcgaggtgg cgctgcgcgg
    19441 tcaggtcgag gccgaactgc aacgcatggc ctacagcgac gcattgaccg gcctgcccaa
    19501 ccggctacag ttctttgagc gggccgacca actgctgaag cgggagcgac gcgaccaggg
    19561 caggctggcg ctctgcttca tcgatctgaa cgatttcaag aagatcaacg accagcatgg
    19621 ccatctggcc ggtgatgccg tgctgagcca gattggccag cgcctgcgcc gggcggttcg
    19681 cgaatcggac atcgtcgccc gctttggtgg cgatgagttc gtcgtgctgt tcgacaacct
    19741 cgacacgccg gccgacctgc cgcggctggt cgatcatctg cagagtgcca ttcgcctgcc
    19801 gatcgaccat cagggcgtca ccctgcacag cggcgccagc atcggcaccg cgctctttcc
    19861 cgatgatggc gatcagatcg atgcgctgct gagccatgcc gaccaggcga tgtatcggcg
    19921 caagcccgcc gccaggtcat cgccaaaagg cgtggtcagc ccactgctgg cgatgccgaa
    19981 tccggcaccg gccgagtagg cgacacggca tggaggaaaa aggctgcccg cggttgcggg
    20041 cagcctgatg cgatcagacg atcttggagt cgcggttgcc ccagtagcgg tctcgcagca
    20101 gccgcttgta gagcttgccg gtcgggtggc gtggcagctc gtcgatgaag tcgatcgagc
    20161 gcgggcactt gatgtgcgag atgttgtcac ggcagaactg gatcagtgcc tgcgcgagct
    20221 cagggctggc gtcgtttttg tccatcagct gcaccaccgc cttcacctct tcgccgaact
    20281 cctcgttggg cacgccgaac actgcacagt cggccacctt ggggtgcatc accagcaggt
    20341 tttcggtctc ctgcggatag atgttgacgc cgcctgagat gatcatgaac gccttgcggt
    20401 cggtcagata gaggtagccc tcctggtcga gatagccgac gtcaccgagt gtgctccagt
    20461 cggtgtggat cgggtggcgc gactcggcgg tcttcttcgg atcgttgtgg tactggaaca
    20521 gcgggccgcc catgccggcg aagtagaccg tgcccgattc gccggccggc agctcgttgc
    20581 cttcgtcgtc acagatgtgg atggcgccgc cggcggtgcg gccgaccgag cccttgtgct
    20641 gcaaccactc cagcggctcg atggcggtgc tgccattgcc ttctgaaccg ccatagtatt
    20701 cgtagatgat cggtccccac cactccatca tctgctcctt gaccgccacc gggcagggcg
    20761 cggcggcgtg gatggcgcag cggtgcgagg agaggtcata cttcttgcga tcctcctccg
    20821 gcagtttcag catgcgcacg aacatcgtcg gcacccactg gctgcaggtg acccggtact
    20881 tttcgatcaa ctgcaacgcc tgttcggcat cgaacttctc catcaccacg atggtgccac
    20941 cgcagcagag cagggtgcgg gtgtagccga gcggcgcggc atggtagagc ggcgccggcg
    21001 agaggtagat gctgttctcg tcgaagccat agcgtgacgg cagcatcgcc atcatcatgt
    21061 tgggcacggt gacgtcggta ccgggcagcg gcttcagaat ccccttgggt cgtccggtgg
    21121 tgccggagga gtagagcatc tcgacgccgg cggtctcgtc ggccagcggc tcggtcgact
    21181 gcgccagcac cgcctcttcg aatgactgat agccggccag cacgccatcg agcatgtagc
    21241 ggttcctgac cgccggcatc agcggcacca gttcggcggc gacatcggcc ttggccatgc
    21301 tggtgatgaa ggcggtggcc atgcagtccg cggcgatgta gtcgacctcg ctggcggtca
    21361 gccgcgagct gatcggcgtg aagtagagcc cggctcggtg ggcgccgaag gtgatgatgt
    21421 gaaaccatgg gtgattttcg aggaacatcg cgacatggtc acccaccttc agaccggccg
    21481 aacgcaacag ccgggagacc tggatcgacc gctcgttcag ctcgcggtag gtgatcacct
    21541 gaccggtgtt ggccatgatg atggcgggct tgtcgggaaa acgggcagca tggattgatg
    21601 gatacatgaa aggctcctga ttgttatagg aatatccggc gcaagcacag aggccggcgc
    21661 ggtacagact gcggttcccg gctgtttctg atggttcgat tgtcgacgac ggctcaaaag
    21721 ccgtcaatgg gatgggcggc agtccgccga ttcagtgcac gcgcggggtc agagcatcgg
    21781 ctgccgcaga tcggcctgct gcaccagcag cagctccatc gacacacggt cgatcggtcg
    21841 gctgaagagg aaaccctggg cctcatcgca gccgatgtcg cgcagatagg ccagctgtgc
    21901 ctcgctctcc accccttcgg ccaccaccgt catgttgagg ttgtgtccca tcgcgacgat
    21961 ggtggagacg atctcggcat tgttgaaatc atccagcagg tcatggatga aggatttgtc
    22021 gatcttcagc gtgtcgatcg gaaaacggct cagatagttc aatgatgagt agccggtgcc
    22081 gaaatcatcg atggcgatgc gcacaccggt cgcggtcagt tgctgaagcg tttccagtga
    22141 ggccttgggg ttggtcatca gcagattttc ggtcagctcc agctccagat ggcgggggtt
    22201 gaatccgctg tcgtcgagca tccgggcaac ccgctggacc agatcgggct gctgcaactg
    22261 caccggcgag aggttgaccg ccagcgtgaa gggatggctc gactgcctga cccagggtaa
    22321 ggcatcttcc atgcccttgc gcagcaccca gtcgccaatc ggcaggatca gtccggtctc
    22381 ttcggcgatc gggatgaact gatcgggcgg caccagaccg cgggtcggat gctgccagcg
    22441 caacagcgcc tcgatgccgg tgatgcgccg gctgttgacg tcgatcttgg gctggtagtg
    22501 cagcagcagc tcattgcgct ccagtgcctt gcgcaggtcg gcctccagtg tcagccgctg
    22561 ccggaaccgc gtgccgaact ccggactgta gatctgatag ctgttgctgc cctgttcctt
    22621 ggcccggtac atcgcgatgt cggcattctt gatcaggctc tcttcgtcct cgccatcgcg
    22681 cgggaacagg ctgacgccga cgctggtggt gacgaacagc tcatgctcgc ccaaccggaa
    22741 cggctggcgg aactgcccca gcacttcatc catcacccgt tgcaggtcgt tgggctgatg
    22801 cagtccggtc aggatcatcg tgaactcatc accgccgagg cgcgccaccg tatcgctgac
    22861 gcgcaccacc cgctccagcc gctgcgccac ctgttgcagc agcaggtcgc ccatggtgtg
    22921 gcccagggtg tcattgatgt tcttgaaacg gttcaggtcg aagaacagca gcgccacctg
    22981 ctcgccatgg cgctgggcct gccgcagcgc caccgtcagc cgatcgtgga acagctcccg
    23041 gttcggcagt ccggtcagat gatcatagta ggccagccgc tgcagccgct cctggatccg
    23101 cggctggttg ctcagcggcg agaagacgat ggcatggagc gtgggcgcgg cctgcgcttc
    23161 gacatgaatg gcactgatct tgacccactg caggatgcgc tcacccgcct gggtgcgggt
    23221 ccacagctca ccggcccact gtccctgggt ggccaggtcg gacctgagct gctcggcagc
    23281 agcggcttcg agcggttcgg ccagcagttc cgccggcgag tggcccagca gtgtctcctg
    23341 ggcatagccg tagttgcgct gaaaagcgct gttggtggcg accaccagaa aattctggtc
    23401 gatcacgatc aggttgtcgg agaactggcg cagcacctcc agcgccacct gcagccgccg
    23461 ctgcatctgc ttgtagcccg tgatgtcggt gatggtgccg accatgcgca ccggaacgtc
    23521 gtgcatgtca taaagcacct ggccgcgcgc cagcacccag cggtaacggc catcacgcgc
    23581 acgcaaccgg taggagatct gatagtgcgg ttgcgtaccg gcgatatggg cctgcatcgc
    23641 ctgcaggacc tggtcggcat cccggggatg cagccgctgc tgccactcgt gcagatgggg
    23701 ggggaactca tccggctggt aaccgagaat ctccagccag cgtggcgagt agtagacctg
    23761 atcactctgg atgcaccagt cccagatgcc atctctgctg gccagcaacg ccagttgcag
    23821 ccgttcgtac tccttggcat cgaccgcctt gagggcagtc cgcactggag agagcgggtt
    23881 tttgggaggg gccatctgct cgatcactgc cgatactggg ggaggtcttg gaaagtgtag
    23941 cagcggttga cggttctgct ctgcacgacc gggatcagcc caatcctcga ggttatcgcg
    24001 cattgcagtc gtgtgctcgt tcacggagtt ggggcggaac cgggcattga tctcctgcag
    24061 gccccaaggt atggtgaccc tgcggccgtg gtgtgtcgcg ccctctctgc catgcctgaa
    24121 ctgatgttcc cacagccctt cttgagcccg aacaggctgc tccacccaag tggacggcgc
    24181 gcgatggcct gcctgctgct catcaccctg gtggtcgctg gctgcggcca gaatgtaaaa
    24241 aaagaggacc ccagcggact gtcggtcaac ggcatcgacc tgcaggtgaa gatgtcggcc
    24301 aacctcttca tcccgccacc gctgccaccg gttgctgaat cgcagcggcg catctacctg
    24361 atcggcacca acacctcgac tgcccaggcc gccaacgagc ggttgcagcc gctgctgatc
    24421 gagcaactgc tgcgacgcgg ctacctgatc accgacaacc cggccgaggc caggtacatg
    24481 ttgctctaca acgtgctcta tgtcggcaag gaggtgcgcg atctttcgac catcacggcg
    24541 gtgtcggccg gcgtcggcgg tgccgccgtc gccgccacca tcggccgcaa caacgagacg
    24601 gtgcagaatg ccggcattgg tggcctggtc ggcaccgtca ccggtgcggt gaccggccac
    24661 tacttcgaga ccaacacctt cgagcaggtg gtcgatctgc agattcgcga acgccagccc
    24721 gccgtcccgg ctccgctgcc gccggaggag accaagcgcc ttgccgtgga gagggccatc
    24781 cgccaggtca atctggcact gggactgggc gagctccaca ccgaaaccac gcctgaaacc
    24841 gaccagcgac tgccgccatc atccgacaac tggcaactct acagcagcca ggtggtcggt
    24901 tttgccaccc gcctgcggct gcagttcgag gaggccgagc cgctgctgtt ccaggccatg
    24961 gcgcgggaga tcagtggtct gttctgggat ctgcatcaat tgccgggcaa caccgcacca
    25021 cggaactgaa caggtgcgcc ggttcaacgc cagtggtggc tgcgcaccag ttcaagcgcg
    25081 cggctctggg cgcgcagcaa ctgctcctga ttcagcagct gtgccagtgc atcacgcagt
    25141 tgcagcacct cctgccgccc agcaccgctg aagcgcgacg cggcgacacc gacccagatg
    25201 taggcctgct cgaagttctg cggcacgccc agtccccggt aatagaactg cccgagcaga
    25261 atctgtgcat gcagatcgcc ctgttcggcc gcccggctgc accagctcat cgcatcgcca
    25321 taactctgcg gaacgccctc accgctgtaa taggccagcc ccagcagcct ctgcgcctgc
    25381 ggatcgccct gcagcgccgc cttggtgtac cagtgcacgg cactgccgtg atccacggca
    25441 gtgccgactc cctgtgcgta ggcctgaccc agcgccacgg cggcttcggg atgatcggcc
    25501 tcggcagcct gtcgccacca ccgcaccgcc tgcgccggat cgcgtgcgat gccaagaccc
    25561 tgcgcatagg cctgccccag ccgaaactgc gcctgcgact gcccctgcgc ggccgcctcg
    25621 gcaaaccact gtgcggactg gagcaggtcg cgcggcaccc cctctccgcg cagataggcc
    25681 tcgcccagcg ccgactgcgc caccggatgg ccctgctcgg ctgccttggc gaaccactcg
    25741 gccgccaacc gcggatcgcg tgggactccc tgtccgctgt catgggccag cgccagacgc
    25801 agttgaccca ccgcatcgcc ctgttgtgcc gccttgctga accactgtac ggcctgggcc
    25861 tgatcctgcg ccatgccgat gccagaaagc gcggcctcgc ccaggcgcac ctgggctgcc
    25921 gccagcccct gctcggcggc ccggcggtac cactgcactg cctggacggc atcgcgcggc
    25981 acaccgcgcc cggtcagata gccattgccc agttgcaact gggctgatgc gctgccctgc
    26041 ccggccgctg ccgcatacca gtgcatcgcc tcctgccaga gattctgtgc ggccagacgt
    26101 tcggccagca gccattgcgc ctgtatgtcg ccctgctctg cctgttgcgt cggatctgga
    26161 ggtggttcag cgggaagcgt tgccacgggc ggtggttcgc ctgccgctgg tgacggcgcc
    26221 gattcggtcc tgccgaaccc ggcgagcgcc agcagaacca gccccgggag gagagtgcga
    26281 aatcgagaca gcatcatctg cagtggccca tcgaatggaa aaccggccga ccgctcgaaa
    26341 gcattgcgtc gaccaggtgg cagtctggcg cctctaccgc gccacaggca agggcagcac
    26401 atttttttta cgcatgccgg ccgcctgatt gacctggatc aatgccgcat cgggaatggc
    26461 ctgaaatgcg aacccgtcga ggttgattca tcggtagaat gggcagcatc cgcacatgat
    26521 aaacaaaaga ggagtgaccc atgacggcag cacccgcgct cagcaatgtt gagacccaac
    26581 ccgccaccca tgtcgatgta ctgatcgtcg gtgccggcat ttcgggcatc gatgcagcct
    26641 accacctgcg cacccagtgc ccggagaagc gcttcgtcct gctggagacc caggagagct
    26701 tcggcggcac ctggcacacc cacacctatc ccggcattcg ctccgacagc gacctctaca
    26761 ccttcggctt caaatggaag ccctgggtcg gcgccccgat cgccaccgcc gaggagattc
    26821 tcaaatacct caaggagacg ctggacgaga acgacctgca ccagcacatc cgttaccgcc
    26881 acaaggtgaa gagcgccagc tggaacagcc agaagcagca gtgggcggtc accgtcgagc
    26941 agctcgacag cggcgagctg cggctgttca cctgcaactt cctctggatg tgtcagggtt
    27001 actaccgtca tgagcagggc tacactcccg actggccggg catggagcgg ttcaaggggg
    27061 tgatcgtgca tccgcagagc tggccggccg atctcgacta taaaggcaag cgggtcatcg
    27121 tgatcggctc gggcgccacc gccgcgacca tcgtgccggc gatggccgcg gagtgcgcgc
    27181 acatcaccat gctgcaacgc tcgcccacct acttcacccc cggcgccaac cgcaacgaac
    27241 tggccgacct gctgcgcgag ctggagattc ccccggagtg gacccacgag atcgtccgcc
    27301 gcaaggtgct gaaggattcg ggccagatcg cccggctggc cttcgagaag cccgaagagc
    27361 tgaaggccca gctgatcggc gtggtgcggc agcagctggg cgatgagtac gatgtcgaga
    27421 cccatttcac acccaagtac cagccgtggc gccagcggct cgccttcgtg cccaacggtg
    27481 acctgttcca gggcatccgc tcaggcaagg cctcggtggt gaccgaccag atcgagacct
    27541 tcaccgagaa gggcctcctg ctgaagtcgg gccaggagct cgaagctgac atcatcgtca
    27601 ccgccaccgg cttcgacctc tgcctgctgg gtgatgtgca gttcaccgtc gacggcaagc
    27661 cggtcaactt tgccgacacc gtcacctacc gtggctgcat gttcagcggc gtgcccaaca
    27721 tggcctggat cttcggctac ttccgcgcca gctggacgct gcgagccgat ctggtctgcg
    27781 aactggtctg ccgcatgttc aacctgatgc agcagaaggg cgccagggtg gtcacgccgc
    27841 aactgcgtga tgaggaccgg acgatggccc ggttgccgtg gatcgatccg gaaaacttca
    27901 acccgggcta cctggcgcgc ggcattcatc tgatgccgaa gcagggcgac catgaaccct
    27961 gggtcttcac ccaggactac catatggaga agagcgagtt tccggccgcc aacctggagg
    28021 atggcgcgct ggtctacagc tgaaagcggc ctgactggcc gccgtttccg atgctccaaa
    28081 aagcaagagg ctgccgaagc agcctcttgt cgtttgagtg ggtcggcatg gcgccgaccc
    28141 agatcgacga tcaacccgcg aatgggcact tctccacgga ggccgagccg ctctggctgc
    28201 cc
//
LOCUS       DAORCQ010000025        28000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_932_length_28000_cov_5.612739, whole genome shotgun sequence.
ACCESSION   DAORCQ010000025 DAORCQ010000000
VERSION     DAORCQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 28000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28000
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_932_length_28000_cov_5.612739"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKI23_04225"
     CDS             <1..414
                     /locus_tag="PKI23_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNN36183.1"
                     /translation="VGGLEQVVGRVHVVAREVVEHRAVANGGEGQRGLGQTEVEIGTA
                     TGPGTLLQVRSQDQAATTQRRDPTAERPSTQKTATQRVDYRVRKGDTLYRIASRFKLP
                     VEKILEWNESVRKQSIKPGDQVTLYVEPQKLPAES"
     gene            518..829
                     /gene="fis"
                     /locus_tag="PKI23_04230"
     CDS             518..829
                     /gene="fis"
                     /locus_tag="PKI23_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HNN36184.1"
                     /translation="MSNEMEPLVEVSRRRPPRAKRGRTQLQDAQTLRDCVEIAMNNYF
                     ENLDGQAVSDVYEMVLSEIEPPLLEAVLRHVHHNQTKASQVLGLNRGTLRKKMKAYGL
                     L"
     gene            1028..1801
                     /locus_tag="PKI23_04235"
     CDS             1028..1801
                     /locus_tag="PKI23_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNN36185.1"
                     /translation="MEINGPVLLEQREGVALLTLNRPPANSMNLQAFIELEQLLDTLA
                     RDPQVRAVVLTGAGDKGFCAGFDLTDTANSTTVSLKAQAVCNQIEAFPKPVVAAINGY
                     ALGGGCELALACHFRFMVAAPKAVIGLPETQLGVIPVWGGTQRLPRLIGRSRGTQMIL
                     LAERIDAEEAAQIGLVDRVCAPAELFQQAFAFAVALAKRPPLATRAVLRAIHIGLNHG
                     MAAGLQAELDEVKGVRGSKDAEEGIRAFFEKREPRFSGE"
     gene            1835..3010
                     /locus_tag="PKI23_04240"
     CDS             1835..3010
                     /locus_tag="PKI23_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNN36186.1"
                     /translation="MYRIQLLNQISRKGTDRFARERYELGGQVEAPDAILLRSHKLAV
                     SELGANLKAVARAGAGVNNIPVSVLSERGIPVFNTPGANANAVKELVLCAMLLASRGI
                     VPGLRYVETLAGMTDVAALNATLEQEKKRFAGQELAGRTLGVVGLGAIGSRLTDAALH
                     LGMTVLGYDPALSVEAAWRVPNQVQKMDNLASLLARSDYVSLHVPLLENTRNLLREET
                     LAYCKKGAVLLNFAREGVVDESAVVKFLDSGYLRQYVADFPHPLLMGRSDVLLLPHLG
                     ASTLEAEENCAVMAADQLIDYLETGNIRNSVNFPTLVLERNGGCRLAFSNRNVPKILG
                     KVLSLLADHNLNVLDMLNKSRDEIAYNLIDVECEIPPALLPAIRAVEGVIDLRVIPG"
     gene            3067..4356
                     /locus_tag="PKI23_04245"
     CDS             3067..4356
                     /locus_tag="PKI23_04245"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNN36187.1"
                     /translation="MTLIRQLADRARQSREQLLRERRMATAEMPLKYWCQQAHFTTPA
                     SGTPEKAIQGLAAARKLLQKFDISPAELADHAGAPLSALRGLIEQPDCHTPLVMVDCE
                     DAVALDAESARKARAGAIRCFSQANWGSTLAFFRPAGLTLDGCIGDLFEVLPAVASER
                     VEHYPIDGLIWPKVEHPNELVWLNERLGEIEHLLGLPEQRIKLQFLVESGYALAQLPE
                     LVKAVTPRLTGIIWGIADYAADVNLPELRNDHPSCDWARQVIVNVAGAVNVPAIDNMT
                     LNYPTPLHRGEGLSLAQQQANKLKVLNALKEAYQDACHGIRLGMSGKWVGHPGQLLMV
                     LAAYQGACSQAQLDQDIAEIELYQRSVASGSGATMIGRGEGDYMADRATDRHLRARLR
                     RATAWGRLDAQRAASLGIINENEFWQLDARANPASEA"
     gene            complement(4357..5835)
                     /gene="malQ"
                     /locus_tag="PKI23_04250"
     CDS             complement(4357..5835)
                     /gene="malQ"
                     /locus_tag="PKI23_04250"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNN36188.1"
                     /translation="MSADCMDAPGNPGSPLARRRSGVLLHLTSLPGSQGCGTLGEAAC
                     RFVDFLAESGFGVWQMLPLGPTHDDGSPYLCLSAHAGNPRLIDLQALVQHGWLDSDLT
                     STESPLLRAWLRFKATASPTEQTEFEQFRNSAAGWLPDYALFMALRSHFNDHSWVHWP
                     TPLRQRDPAALKQARHHHADAIEAICFEQFLFDSQWRQLHRHARERGVALFGDIPIFV
                     DHNSADVWANQRQFDLDADGNPRVVAGVPPDYFSATGQRWGNPLFRWEEMEADGFSWW
                     QGRVATQMRYFDLLRIDHFRGFEACWEIPADEPTAMVGRWAPARGEALFQSLIERHGT
                     LPWVAEDLGTITPEVERLMARFDFPGMKILQFAFDGGADNPYLPHNHRQQSVVYTGTH
                     DNDTSLGWYQGLTPTLQERVDEYLGRPGEAMPWPLLRAALASVARLAIIPMQDLLGLD
                     SAHRMNQPGTVEGNWRWRFAWSDLPADLSERLRRLNQLYGRG"
     gene            complement(5832..7547)
                     /locus_tag="PKI23_04255"
     CDS             complement(5832..7547)
                     /locus_tag="PKI23_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNN36189.1"
                     /translation="MSAESFTPVVLMWHMHQPHYRPLNASAFRQPWSYLHAIKDYVDM
                     AAILEQTPGARSVVNFAPVLLEQIDAYGEQIESFLAGNGEIADPLLAALASEQLPSDG
                     ESRLLLTSQSLRANEQRLIQRFAPFGQLAALARHLLAHPGQVGYLHDQFLWDLLVWYH
                     LAWLGETVRRGDSRITALMEQGNHYSLAQRRLLLSIIGELVQSVIPRYRALAEGGQIE
                     LAMSPYGHPILPLLLDIDSAREAMPTATLPAADHYPGGAERARWHLRHGIETFSRYFG
                     MIPIGCWPSEGAVSDATLQLLAAAGFRWAATGESVLRHSLTAAGRMGDACIHHPYRVD
                     GIDIACFFRDDQLSDAIGFKYANWHGDDAAADLVQHLENIAAACGNDPSRVISIILDG
                     ENAWEYYPNNGYYFLTALYQKLAQHPTLRLTTFGNLVKNRPKRPPPDRSLQLPSLVAG
                     SWVYGTFSTWIGSPDKNRGWELLIEAKQQFDRVIAAGTLSAERQQAAEQQLALCEGSD
                     WFWWFGDYNSAESVSDFERLFREHLVSLYQTLMIEPPQSLTQVISKGGGAPEQGGTMR
                     RGSET"
     gene            complement(7537..8850)
                     /gene="glgC"
                     /locus_tag="PKI23_04260"
     CDS             complement(7537..8850)
                     /gene="glgC"
                     /locus_tag="PKI23_04260"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNN36190.1"
                     /translation="METTKPTAKGKKRSSSPTAPRRFVSRITGETLALVLAGGSGSRL
                     HNLTRWHAKPAIPFGGKYRTIDFSLSNCVNSDVRRIGILTQYKSHSLINHVQKGWSFL
                     RAEMNEFIELLPAQQRIHDSWYKGTADAIYQNLDIIRLHNPEYVLILAGDHIYKMDYG
                     LLIAQHVEREADMTVCCIETSLAEASEFGVMSIGADERIIGFQEKPKHPIPMPGSPDK
                     ALVSMGIYVFNTGFLYEQLIKDAETPRSSHDFGKDIIPSAIDRYRVFAYPFRDVENGE
                     QAYWRDVGTVDAFWQANMELLDVTPPLNLYDQEWPIWTHQEQLPPSKFVFDDDDGRRG
                     MAVDSMVSAGCIVSGALVRRSLLFNNVRVNAYSELNQCVVLPRAVIGQHCRLTRAIID
                     QECIIPDGTVIGEDAKEDARRFHLSPEGIVLVSPEMLGQEVRHVR"
     gene            complement(8975..9220)
                     /locus_tag="PKI23_04265"
     CDS             complement(8975..9220)
                     /locus_tag="PKI23_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36191.1"
                     /translation="MHHLEITPWMAAKITERIVMAGLDDAPLGFIEHHGNGRRNRRRR
                     RSHVDDLAELNCWSSALKQKSAPRKNRRRSAIKPAAG"
     gene            9254..11284
                     /gene="glgB"
                     /locus_tag="PKI23_04270"
     CDS             9254..11284
                     /gene="glgB"
                     /locus_tag="PKI23_04270"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNN36192.1"
                     /translation="MTSNGAQEPLERLGRSDLFSWQGSADTLPKHYLLRWVDRWGVEH
                     EAHDPYSFGPQLLEFDLALFAAGKHWHAYRFLGAHCREIDGISGVFFAVWAPNAERVS
                     VVGDHNAWDGRHHPLRCRGASGVWELFIPGLAAGLHYKFEIRARNGAILLKSDPYAQG
                     SELRPATASVVREPSRHQWQDAQWLDQRQRHDWLHAPLSIYEVHLGSWRRDEAGHFLN
                     YRDLAHGLVDYIAQQGYTHLQLLPITEHPFDGSWGYQSTGYFCPTRRFGEPDELRYLV
                     DLCHRHGIGVLLDWVPAHFPRDGHALAWFDGSNLYEHADPRRGEHRDWGTLIFDYGRN
                     EVRNFLLSSALYWIEEFHIDGLRVDAVASMLYLDYSRQPDDWLPNIHGGNENLEAIDF
                     LKHLNGVVHERFPGVLMMAEESTAWPMVSRPVHLGGLGFSMKWNMGWMNDTLDYIGKD
                     PIHRLYHHDKLTFGLLYAFTENFILPFSHDEVVHGKRSLLYKMPGDEWQRFASLRLLL
                     TYQFTQPGKKLNFMTNDIAQGREWNHDRELEWYLLQYPFHQGVQRLVADLNRLYRNEP
                     ALHRHDFDSSGFEWIDCHDSTQSVLSFLRKSDQGWLLVLLNFTPVVREDYRVGVPAAG
                     SYRELFNSNSRHYGGSDLGNGQVVTEPVAWMGRPVSLRLTLPPLAGIVLRLA"
     gene            11304..12941
                     /gene="glgA"
                     /locus_tag="PKI23_04275"
     CDS             11304..12941
                     /gene="glgA"
                     /locus_tag="PKI23_04275"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNN36193.1"
                     /translation="MAKAKARPRKKPPAALDVLFVASEAHPLIKTGGLADVAGSLPIA
                     LTQLGQRLHLLLPAYAGVKRQSGALLERARFTLAHGRCGEVEVRLLEGRLADSEVILW
                     LVDVPEYFAREGGPYQDAAGQDWPDNAERFALFCRVAALLAIDQLGLGWRPDIVHLND
                     WQSALTALFLQPFPFRPRLLFTIHNLAYQGLFSRAQFNLLDLPESAWSIEGVEYYGQL
                     SFIKAGINCSDWISTVSPTYAREITTFAYGCGLDGLLRARQGVLVGILNGIDAQVWNP
                     ATDPLIPHHYSADELSGKRLDKVALLRRMGLPADDELPLLAMVTRLVEQKGIDLLLEI
                     LPWLMTQPLRLVVLGNGDPHYTELLLGCARRHPDRLAVVAGYDEPLAHQIEAGADLFV
                     MPSRFEPCGLNQLYSLRYGTLPLVRRVGGRHRHRCRCGNRGQRLLFRLRQRSGTACHA
                     GAGAGALSSTGALATTDALGDGGRFLLAAQLGRLPRALSADARAAVRLRVAAVHPAAT
                     QCAGGSLHSGILLCFSRIFSIRSCIFSLILSRSLWSM"
     gene            complement(12848..13150)
                     /locus_tag="PKI23_04280"
     CDS             complement(12848..13150)
                     /locus_tag="PKI23_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNN36194.1"
                     /translation="MRIKPLLLSTSLICALTAPVWAMSHGDEGGPDHQMMWKQMDSDG
                     DNRISKAEFTKSTEARFDKADANHDGYIDQSERDKMREKMHERMEKMREKHSKMPE"
     gene            13314..14285
                     /gene="cmoB"
                     /locus_tag="PKI23_04285"
     CDS             13314..14285
                     /gene="cmoB"
                     /locus_tag="PKI23_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HNN36195.1"
                     /translation="MIDYEKLWAPLARALPADGCGAIEQATAAQWHAARHGDLPRWQA
                     AVENLPAVESAQLGIDRGAVSLSSPQPLSPAETERLRSGLLALHPWRKGPFNLFGVEV
                     DSEWRCQLKWARIERAIDPLVGRRVLDVGCGNGYYSWRLALAGAELVVGIDPGLLFTL
                     QYWAVARYLPRRTAWVLPLALEALPEGEANFDIVLSMGVLYHRRSPVEHLERLRQLLR
                     PGGQLLLETLVVEGDRQTLLLPPGRYAKMRNVWFIPSVAALGNLLGRCGFRDVELVDV
                     SLTDHNEQRRTDWMRFESLADFLDPADTTLTIEGHPRPRRALLVARR"
     gene            complement(14289..15239)
                     /locus_tag="PKI23_04290"
     CDS             complement(14289..15239)
                     /locus_tag="PKI23_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN36196.1"
                     /translation="MALLEVRDLSRWFDLERQRRLLAVDRVSLTLQPDEVVGLVGESG
                     SGKSTLGRCLIGLLEKSAGEVWFDGERLPARYRTEDFRRHGSAMRMIFQDPYSSLNPR
                     MTVLEIVGEGLRLQGERDRGRIRDQVAHWLQRVGLSAEQMGRYPHEFSGGQRQRIGIA
                     RALISRPRFVVCDEPISALDVSVQAQVVNLLAALRAEMGLTLLFIAHDLSMVRHLCDR
                     VAVMYLGQLVELGPTEQLFSQPRHPYSRLLIESNPIADPQRERARQLPLIQGEMPSPI
                     DLPPGCRFAGRCPQALPICHREPPVARPVDPHHWRACHLD"
     gene            complement(15239..16264)
                     /locus_tag="PKI23_04295"
     CDS             complement(15239..16264)
                     /locus_tag="PKI23_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN36197.1"
                     /translation="MAERLLEVTDLAVDFASDGGRVQAVRGVDFGVEAGRTLAIVGES
                     GCGKSVTVQALMGLVPMPPGRILRGSARFFGEDLLTLAPRRANALRGARIGMVFQDPM
                     TSMNPTMRVGDQIAETLRLHRGLSPAAATRQAVELLEQCRIPDPQRRARNYPFEFSGG
                     MLQRAMIAMAIACQPALLIADEPTTALDVTIQAQILRLLQALQARTGMAMILITHDLG
                     VVARMADEVAVMYAGRIVERGSVESIFRRPAHPYTLGLQQAMPDPASRAPLRAIAGAP
                     PDLFQPPTGCGYHPRCPHALRICQREEPPLQPLDADVSARCWLHHPQAPAVAPLHQPL
                     RDDQGSP"
     gene            complement(16268..17659)
                     /locus_tag="PKI23_04300"
     CDS             complement(16268..17659)
                     /locus_tag="PKI23_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN36198.1"
                     /translation="MAGPLAADDFLPLPPQPAMERAVRPSRSYWQDGWRRLRQNRSAF
                     AALGLVLGLLLFALAGPLLWPVDPAAQDLEQLSRPPTLGERALLVDLSSEWSGTPAAE
                     SEVGAPLPAARLGRSSALSVVGSPTSQSVRLQWQAVPGARGYILYRTDHPPMEGDDLG
                     LPLVRIIGGSEVSHEDRLNPQPGRHYYSVVATDGVDEADGYRTLAVEVQLGLTLDEAR
                     RRGHELPAGASVTLAPHPLGTDYLGRDLLARLIHGARISLFIGLTAPLLSIVIGAFYG
                     GVAGYLGGRTDALLMRFADFVVALPFLLFMILFRIGFGIGPGESGIAPLLLAMVLLGW
                     PGTARLVRGQILRIREEGYVQAARLMGAGTPYLVLRHMLPNTMGVILVSLTFAVPSAI
                     FTEAFLSFIGMGVAPPTPSWGSMCNDGIKTMLTHPHELLFPALVISLTVLAFNLLGDG
                     LRDALDARLRGRE"
     gene            complement(17663..18595)
                     /locus_tag="PKI23_04305"
     CDS             complement(17663..18595)
                     /locus_tag="PKI23_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN36199.1"
                     /translation="MHRYLLRRLVEGVITVWFIATATFFAMHLVPGDPLLNDKAVPKE
                     IRANLEQHYGLDQPLPVQYLRYLGQMVRGDFGISFTQKNRAVNDIIREHFPVSASLGL
                     VALAIAAAGGILWGALTARYRGRWPDALIMLGVIVAISVPGFVVAALAQLAIVELNRL
                     AGRSLLPVAGWGEWRHFVVPALVLGLGTMAYLTRLMRSSMLEVIHADYIRTARAKGLG
                     EARIFFRHQLRNAILPVITVLGPAVAAITTGGFVIELVFAIPGLGRYFVQAVQQLDYT
                     VIMGTTVFYGAFLVFMVIAIDILYGFIDPRIRVG"
     gene            complement(18652..20298)
                     /locus_tag="PKI23_04310"
     CDS             complement(18652..20298)
                     /locus_tag="PKI23_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNN36200.1"
                     /translation="MSKREQLPPPRVLIKAMLAPLWLLCCTLLAPLAMADAVDAARQK
                     ITLLLGQEPPSLNSIKATDQVSFFILGHTHEGLLRYDAQGQLVGGVARDWKLDERSAT
                     FWLRDDARWSDGQPVTAHDFVFAWQRVVDPAMASEYASILYPIKNAKAISEGRLPPEQ
                     LGVRALDERTLQVDLEQPTAYFLQLTAFGVYLPIREDFYRAQGTRYGAEQDRLLYNGP
                     FRLARWVHGARLTLVKNEQYWNRAQTVLNEIDLPYVTSDSTAAYNLFKDNKVALVGLS
                     AEELNDALRQRVKIRIFPAGSLFYLEFNHRAGRITGNRHFRRAIQRVADPQELVNRVV
                     GVPGTLPGRSLFPVWIKGVNGSFRKEFPAPRVTIDPNQARQELELARQQLGLERFPPL
                     VYLTGDSPTAAKQGEYLQNLLRTQLGLELKIDKQIFKQRLAKMLAGEFDLVAAGWGPD
                     YDDIMTFGDLFASWNINNRGRYANPDYDHWVAVARDATDPRLRMEAMAALQRLVIEDA
                     VIIPQYESGSVYLQHRQLKGVVRRVIGTDPDFTQARVVAP"
     gene            complement(20453..21265)
                     /locus_tag="PKI23_04315"
     CDS             complement(20453..21265)
                     /locus_tag="PKI23_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNN36201.1"
                     /translation="MVIKKAALSLSAMLIAAPAMAGVMFTNGGFENGNLDGWQFSSDG
                     GTASYVSATAGATSFASSGNGGLSSNLVTPGADPIVGINRTYGGSYGVRTGDSLAWGY
                     SGGGTIYNRIRQTATVAAEADGSAGFLYFAWAAVEEISGHSTTSTPFFQVSVRNNTQS
                     SSIYNVQHYETDGGAWVTAGGWKYSANSNPSDPLGWNVVGLDLAALGVNVGDSLTLEA
                     IARDCTPSAHAMYVYLDGFGGTPPVQGVPEPASLALMGLGLAGIGMARRKKA"
     gene            complement(21570..22865)
                     /gene="argA"
                     /locus_tag="PKI23_04320"
     CDS             complement(21570..22865)
                     /gene="argA"
                     /locus_tag="PKI23_04320"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNN36202.1"
                     /translation="MNGSDIDYVKWFRHSSPYINAHRGRTFVLMLSGETVAHANFIHI
                     VQDIALLNSLGVRLVIVHGARPQIEAALEQRGLRSQFHDHLRITTLELLEAVKQAVGI
                     LRSEIEALLSQSSQQRLRLGVISGNFVTAKPHGVHDGIDFHHTGEVRGIDHAAIHRHL
                     AAGEVVLLSPLGYSPTGEIFNLSFEQVAAETAIALRADKLIAYSEKPGVLDRDGQLLR
                     ELTPSEIRQRLDDASLTSSPALRGCLHASSAGVQRSHLIGFGEDGALLRELFTRDGAG
                     TLISHERYEKIRPALIDDVAGIIELIRPLEEDGTLLRRSRERLEEEIGQFSVIDRDGS
                     VIGCAALYPFSDGHSGELACVVVHPDYRSGERGDLLLEQIEKRARQQGIARLFVLTTR
                     TAHWFRERGFVPATVDDLPDEKRSLYNYQRNSQVYCKGL"
     gene            complement(22877..24058)
                     /gene="argE"
                     /locus_tag="PKI23_04325"
     CDS             complement(22877..24058)
                     /gene="argE"
                     /locus_tag="PKI23_04325"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HNN36203.1"
                     /translation="MRLRHLPSLTEQLAQLVATPSVSATSAAHDLGNGAVIELLADWL
                     DRLGFAIEVTEAGRGPGGAAKRNLIATLGSGPGGLVLAGHTDTVPCDPELWRSDPFRL
                     TEREGRLHGLGSCDMKGFFPLAIEAARQFAGQQLNAPLIILATADEESSMCGARQLVA
                     LGRPRGRHAVIGEPTGLRPVRLHKGITMESVRVVGQAGHSSDPALGRNAIEGMQQVIT
                     LLLELRDELGAQFHNSAFAVPTPTLNLGCIHGGDNPNRICGDCELQFDLRLLPGMQID
                     TVRQMIGERLDRLSRQLGMPIDFQPLFPGLPAFETAADAGLVRLLERLTGHASEAVAF
                     GTEAPFLTELGLETVVFGPGEIDCAHKPDEFLALDAIRPCVDLLTDLIGQLCLAPATT
                     R"
     gene            24196..25992
                     /locus_tag="PKI23_04330"
     CDS             24196..25992
                     /locus_tag="PKI23_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNN36204.1"
                     /translation="MKSSESLSQSADRKLDLRRLLEALQAQGRLSRSDFEQALGKPRD
                     QAAVALHPLVWIARQQYGDAQQSGRTLDLETLTQWLAQQIPDQQYYHIDPLKIDVAAI
                     TEVMSFAFSQRHQILAVEVQPQEVVVASAQPLVQGWEPDLAHVLRKPIRRVVANPEEI
                     ERLSVELYTLARSVNSASARGAGPSHPGLGNLEQLLEIGQLKSPDANDQHIVNIVDWL
                     LQYAFDQRASDIHIEPRRDVGNLRFRIDGVLHTVYQFPSAVTAAVTSRLKALGRLNVA
                     EKRKPQDGRLKTRAPAGNEVELRISTLPTAFGEKMVLRIFDPEVLLRSFAQLGLTADD
                     FTRWEGMINQPNGIVLVTGPTGSGKTTTLYSSLKRLATPQVNLCTIEDPIEMIEPTFN
                     QMQVQHNIDLSFASGVRALLRQDPDIIMIGEIRDLETAEMAIQAALTGHLVLSTLHTN
                     DAPTAVTRLLELGVPPYLVKATLIGVMAQRLVRILCPHCKRAEPLDLDAWQALIHPFK
                     VNPPARMCVPVGCLECRETGFLGREGIYEILTLSDLVREQITAQCDLTALRHMGLREG
                     MHTLRLSGARKVAAGLTTVAEVLRVTPNEMRG"
     gene            complement(26117..26404)
                     /locus_tag="PKI23_04335"
     CDS             complement(26117..26404)
                     /locus_tag="PKI23_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNN36205.1"
                     /translation="MIYTARALADLDRLTDFLVEADPESAVQTVGLITEAMQVLANHP
                     LIGRPAEQALRELVISRGKSGYLALYSHEVEQDIVLVLAIRHQREAGHVPE"
     gene            complement(26401..26685)
                     /locus_tag="PKI23_04340"
     CDS             complement(26401..26685)
                     /locus_tag="PKI23_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36206.1"
                     /translation="MSTTSLKIPDDVKQLAVAAAKQQGITPHAFMVDAIRTAAANAQR
                     RARFVTDAIASREEAIKSGEGYASEDVHAYLQARAQGKSLPKPRAHSWRA"
     gene            complement(26855..26931)
                     /locus_tag="PKI23_04345"
     tRNA            complement(26855..26931)
                     /locus_tag="PKI23_04345"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26895..26897),aa:Asp,seq:gtc)
     gene            complement(26947..27022)
                     /locus_tag="PKI23_04350"
     tRNA            complement(26947..27022)
                     /locus_tag="PKI23_04350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26987..26989),aa:Val,seq:tac)
     gene            27183..27539
                     /gene="queD"
                     /locus_tag="PKI23_04355"
     CDS             27183..27539
                     /gene="queD"
                     /locus_tag="PKI23_04355"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNN36207.1"
                     /translation="MEIYKEFVFEAAHRLPAVPAGHKCGRLHGHSFRVRVVVEGTPDP
                     VSGWLIDFAEIKARFAPLYAQLDHNYLNEIPGLENPTSEQLAIWIWQRLKPLLPALCR
                     IELQETCTSGCIYSGV"
     gene            complement(27541..27996)
                     /locus_tag="PKI23_04360"
     CDS             complement(27541..27996)
                     /locus_tag="PKI23_04360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNN36208.1"
                     /translation="MTVSTQVDFYLLAEGSPHARDLFVCRLVEKAHRLGHAILLLISD
                     QPQRAKLDALLWSFRAESFLPHGEQMADEGQRIFLDDRIRLDRCDDLLINLTDALPGG
                     FAGFNRVAEIVTSDPLQRELSRQRFRAYRQTGIQPVTHDLTGETRPQPR"
BASE COUNT         4830 a         8812 c         9411 g         4947 t
ORIGIN      
        1 gtcggcggtc tcgaacaggt cgttggtcgg gtacatgtgg tcgcgcgtga ggtggtcgag
       61 catcgggccg tggcgaacgg cggcgaggga cagcgcggtc tcggccagac tgaggtcgag
      121 ataggcaccg cgaccggtcc gggcacgctg ctgcaggtgc ggtcgcagga ccaggcagcg
      181 acaacgcagc ggcgtgaccc gactgctgag cgcccgagca cgcagaagac agcgacgcag
      241 cgggtggact atcgggtgcg caagggcgac acgctctacc gcattgccag ccgtttcaag
      301 ttgccggtcg aaaagattct ggagtggaac gaatctgtcc gtaagcagag catcaagccg
      361 ggtgaccagg tgaccctcta tgtcgaaccg caaaagctgc cggcagagag ctgattcatt
      421 gtctgcgttg ttggcaacga cgtaaacttg ccgaggtctt gatttctgat cgaggctccg
      481 tgatacaggt tcgctggaag cagaaggagt gggaagcatg agcaatgaaa tggaaccctt
      541 ggtcgaggtc agccgtcgcc gtccgccgcg cgccaagcgc ggccgcacac agttgcagga
      601 tgcgcagacc ctgcgcgatt gtgtcgagat cgccatgaac aactacttcg agaatctcga
      661 tggacaggca gtcagcgatg tctatgagat ggtgctctcc gagatcgaac cgccgctgct
      721 cgaggccgta ctgcgccacg tccatcacaa tcagaccaag gcgtcacagg ttctgggcct
      781 caatcggggc accctgcgca agaagatgaa agcctacggg ctgctgtagc acgccgccat
      841 ccgagcgtcg cacggtggca gtgtcgtgct gcgacgctgc cacctcccgc ttcgtctgag
      901 ccgatctccc cgtgcgacag gccatgctgt cgaccgctgt attcccgttg gcacccattt
      961 cgtagaatga tcgggtgggt tgtcgtcccg acccccatcg acattcgaaa aaacaaggag
     1021 tcttcgtatg gaaatcaatg gtccagtgct gctggagcag cgtgaagggg ttgcgctgct
     1081 gaccctgaac cggccaccgg ccaattcgat gaatctgcag gcgttcatcg aactggagca
     1141 gctgctcgac accctggccc gcgacccgca ggtgcgggcg gtggtgttga ccggtgccgg
     1201 tgacaaaggc ttttgcgcgg gtttcgatct gaccgacacc gccaactcca ccaccgtcag
     1261 tctgaaggcg caggcggtct gcaaccagat cgaggccttc cccaagccgg tggtggcggc
     1321 catcaacggc tatgcgctgg gtggcggctg tgaactggcg ctggcctgcc attttcgctt
     1381 catggttgcg gcgcccaagg cggtgatcgg cctgcccgag acccagctcg gggtgattcc
     1441 ggtctggggt ggtacccagc gactgccgcg gctgatcgga cgctcacgtg gcacccagat
     1501 gattctgctg gccgagcgca tcgatgccga ggaggcggcc cagatcgggc tggtggaccg
     1561 ggtctgcgcg cccgcggagc tgttccagca ggcttttgcc ttcgccgtgg cattggccaa
     1621 gcgtccgccg ctggcgaccc gtgccgtgct gcgggcgatt cacatcggtc tgaaccatgg
     1681 catggcggcg ggtttgcagg ccgaactgga cgaggtgaag ggcgtgcgcg gcagcaagga
     1741 tgccgaagag gggatacgcg cctttttcga gaagcgtgaa ccgcgtttct cgggtgaata
     1801 gttcattcca ttgttttgac gagagcagtc acagatgtac cgaatccagt tgctgaacca
     1861 gatctcccgc aagggcaccg accgttttgc gcgcgagcgc tatgagcttg gcgggcaggt
     1921 cgaggcgccc gacgccattt tgctgcgcag ccacaaactg gctgtcagcg aactgggcgc
     1981 gaacctgaag gcggtggcgc gggccggtgc cggggtcaac aacattccgg tttcggtgct
     2041 gagcgagcgc ggcattccgg tgttcaacac cccgggcgcc aatgccaacg cggtcaagga
     2101 gctggtgctc tgcgcgatgc tgctcgcttc gcgcgggatc gtgccggggt tgcgctatgt
     2161 cgagacgctg gccggcatga ccgatgtggc ggcgctgaac gcgaccctgg aacaggagaa
     2221 gaagcgtttt gccgggcagg agctggccgg acggacactc ggcgtggtgg ggttgggcgc
     2281 gatcggttca cggctgaccg acgccgcgct gcatctggga atgacggtgc tgggttatga
     2341 cccggcgctg tcggtcgaag ccgcctggcg ggtgccgaac caggtgcaga agatggacaa
     2401 tctggcatca ctgctggccc gttccgacta tgtctcgctg catgttccgc tgctggagaa
     2461 tacccgcaac ctgctgcgcg aagagacgct ggcctactgc aagaagggcg cggtgctgct
     2521 caacttcgcg cgcgaagggg tggtggatga gtcggcggtg gtgaagtttc tcgacagtgg
     2581 ttatctgcgc cagtatgtcg ccgactttcc gcatccgctg ctgatgggtc gaagtgatgt
     2641 gctgctgctg ccgcatctgg gggccagcac gctggaggcc gaggagaatt gtgcggtgat
     2701 ggcggccgat caactgatcg attatctgga gaccggcaac atccgcaatt cggtcaactt
     2761 tcccacgctg gtgctggagc gcaacggtgg ctgccggctg gccttctcga accgcaatgt
     2821 gccgaagatc ctcggcaagg tgctgtcact gctggccgat cacaacctga atgtgctcga
     2881 catgctcaac aagagccgtg acgagatcgc ctacaacctg atcgatgtcg agtgcgagat
     2941 tccgccggca ctgctgccgg cgatccgtgc ggtcgaaggg gtgatcgatc tgagggtgat
     3001 tccgggctaa tcctggggtg gccggtgctg ccctgaatct tctgaatgcg atggagagcc
     3061 gcgaagatga cgctgatcag gcaactggcc gaccgggccc gacagagtcg cgagcagctg
     3121 ctgcgcgaac ggcgcatggc cacggcggag atgccactga agtactggtg ccagcaggcg
     3181 cacttcacca cgccggccag cggcacaccg gagaaggcga tccaggggtt ggcagcggcg
     3241 cgcaagctgt tgcagaagtt cgacatcagc ccggcggagc tggccgacca tgccggtgca
     3301 ccgctgtcgg cgctgcgcgg gctgatcgag cagcccgact gccacacccc gctggtgatg
     3361 gtcgattgcg aggatgcggt tgcgctcgat gccgagtcgg cccgcaaggc gcgtgcgggc
     3421 gccatccgct gtttttcgca ggcgaactgg ggttcgacgc tggccttctt tcgcccggcc
     3481 ggcctgacgc tcgatggctg catcggcgac ctgttcgagg tgctgccggc ggtggccagc
     3541 gagcgtgtcg agcactatcc gatcgatggc ctcatctggc cgaaggtgga gcatcccaac
     3601 gagctggtgt ggctgaatga gcggctgggt gagatcgagc atctgctggg attgccggag
     3661 cagcggatca agctgcaatt tctggtcgaa tcgggctacg cgctggcgca actgcctgaa
     3721 ctggtcaagg cggtgacgcc acggctgacc ggcatcatct ggggcattgc cgactatgcg
     3781 gccgacgtca atctgcccga gctgcgcaac gaccatccga gctgcgactg ggcgcggcag
     3841 gtgatcgtca acgtcgccgg agccgtcaat gtgcccgcca tcgacaacat gaccctcaac
     3901 tatccgaccc cgttgcatcg cggcgagggg ctcagcctgg cccagcagca ggcgaacaag
     3961 ctcaaggtgc tgaatgcgct gaaagaggcc taccaggatg cctgccacgg cattcgactg
     4021 ggcatgtcgg gcaagtgggt cggccatccc ggccagctgc tgatggtgct ggcggcctac
     4081 cagggtgcct gttcgcaggc gcagctcgat caggacattg ccgagatcga gctctaccag
     4141 cgcagtgtcg ccagcggctc cggcgccacc atgatcgggc gcggcgaagg tgactacatg
     4201 gccgatcgcg ccaccgatcg tcatctgcgc gcccggttgc ggcgcgccac cgcctggggg
     4261 cggctcgatg cgcagcgggc agcgtcgctc ggcatcatca atgaaaacga attctggcag
     4321 ctcgacgcgc gggcgaaccc agcttcggag gcttgatcag ccgcggccgt agagctggtt
     4381 gaggcggcgc agccgttcgc tcaggtcggc cggaaggtcg ctccaggcga aacgccagcg
     4441 ccagttgcct tcgacggtgc cgggttggtt catgcggtgt gccgagtcga gaccgagcag
     4501 atcctgcatc gggatgatcg ccagccgcgc caccgaggcc agcgcggcgc gcagcagagg
     4561 ccacggcatc gcctcgcccg gtcggcccag atactcatcg acccgttctt gaagcgtggg
     4621 cgtcagcccc tgataccagc caaggctggt gtcgttgtcg tgggtgccgg tgtagaccac
     4681 gctctgctgg cgatggttgt gcggcagata ggggttgtcg gcgccgccgt cgaaggcgaa
     4741 ttgcagaatc ttcatgcccg gaaagtcgaa ccgggccatc aaccgctcca cttcgggcgt
     4801 gatggtgccg agatcctcgg ccacccaggg cagcgtgccg tggcgctcga tcaggctctg
     4861 gaacagtgcc tccccgcgtg ccggggccca gcggccgacc atcgcggtgg gctcatcggc
     4921 cggaatctcc cagcaggcct cgaagccgcg aaagtggtcg atgcgcagca ggtcgaagta
     4981 gcgcatctgg gtggcgaccc ggccttgcca ccaggaaaaa ccgtcggctt ccatctcctc
     5041 ccagcggaac agcgggttgc cccagcgctg gccggtggcc gagaagtagt cgggcggcac
     5101 cccggcgacg acgcgtggat tgccgtcggc atcgaggtcg aactgccgct ggttcgccca
     5161 gacatcggcg ctgttgtggt cgacgaaaat cggaatgtcg ccgaacagcg ccacgccgcg
     5221 ctcccgggca tggcggtgca gctgtcgcca ctggctgtcg aacaggaact gctcgaaaca
     5281 gatggcctcg atcgcgtcgg catggtggtg ccgagcctgt ttcagcgcag ccggatcacg
     5341 ctggcgcagc ggtgtcggcc agtgcaccca ggagtggtcg ttgaagtggc tgcgcagcgc
     5401 catgaacagc gcatagtcgg gcagccagcc ggcagcgctg ttccggaact gttcgaactc
     5461 ggtctgttcg gtcggactgg cggtggcctt gaaccgcagc caggcgcgaa gcaacgggga
     5521 ttccgtggag gtcaggtcac tgtcgagcca accatgctgc accagcgcct gcaggtcgat
     5581 cagccgcgga ttgccggcat gggccgacag gcagagatag ggtgaaccgt cgtcatgggt
     5641 cggccccagc ggcagcatct gccagacgcc aaaacccgat tcggcgagaa agtcgacgaa
     5701 gcggcaggcc gcctcgccca aggtaccgca cccttggctg cctggcagcg aggtcagatg
     5761 cagcagcacg ccggagcggc gtcgggccag gggtgaaccg gggttgccag gcgcatccat
     5821 gcagtcggca ctcacgtttc gctgcctcgg cgcatggtgc cgccctgctc cggcgcgcca
     5881 ccgcccttgg agatcacctg ggtcaggctt tggggcggct cgatcatcag tgtctgatag
     5941 aggctgacca gatgctcgcg gaacagccgc tcgaagtcgc tgaccgattc ggccgagttg
     6001 tagtcgccaa accaccagaa ccagtcggag ccctcacaga gtgccaattg ctgttcggcg
     6061 gcctgttgcc gttcggccga gagcgtgccg gcggcgatca cccgatcgaa ctgctgcttg
     6121 gcctcgatca gcagctccca gccgcggttc ttgtccggac tgccgatcca ggtcgagaag
     6181 gtgccataga cccagctgcc ggccaccagg ctgggcagtt gcagactgcg atcgggaggt
     6241 gggcgcttcg gccggttctt caccaggttg ccgaaggtgg tcaggcgcag cgtcggatgc
     6301 tgcgccagtt tctggtagag cgcggtcaga aagtagtagc cgttgttggg gtagtactcc
     6361 caggcattct cgccatcgag aatgatgctg atcacccgac tcggatcatt gccacaggcc
     6421 gcggcgatgt tctccagatg ctgcaccaga tcggcggcgg catcgtcgcc gtgccagttg
     6481 gcgtatttga aaccgatggc gtccgagagc tggtcatcgc gaaagaagca ggcgatgtcg
     6541 atgccatcga cccggtaggg atggtggatg caggcgtcac ccatacggcc cgctgcggtc
     6601 aggctgtgac gcagcaccga ttcgccggtg gcggcccagc ggaagcccgc ggcggccagc
     6661 agttgcagcg tggcgtcgct gaccgcccct tcggagggcc agcagccgat cgggatcatg
     6721 ccaaagtaac ggctgaaggt ctcgatgcca tgccgcagat gccagcgcgc ccgctcggcg
     6781 ccgccgggat agtggtcggc agcgggcagg gtggccgtgg gcatcgcctc gcgggccgaa
     6841 tcgatgtcga gcagcagtgg caggatcgga tggccatagg gcgacatcgc cagctcgatc
     6901 tggcccccct cggccagggc gcggtagcgc ggaatcacgc tctgcaccag ttcgccgatg
     6961 atcgacagca gcagccggcg ctgcgccagg ctgtagtggt tgccctgctc catcagtgcg
     7021 gtgatgcgcg aatcgccgcg gcgcaccgtc tcacccagcc aggccaggtg gtaccagacc
     7081 agcagatccc acaggaactg atcatgcagg tagccgacct gaccggggtg ggcgagcagg
     7141 tggcgcgcca gtgcggccag ttggccgaag ggtgcaaagc gctggatcag ccgctgttcg
     7201 ttggcgcgca gcgactggct ggtcagcagc agtcggcttt cgccatcgct gggcagttgc
     7261 tcactggcca gggcggcgag cagcggatcg gcgatctcgc catttccggc caggaaactc
     7321 tcgatctgct ctccataggc gtcgatctgt tcgagcagca ccggggcgaa gttgaccacg
     7381 ctgcgcgcac cgggggtctg ctcgaggatc gccgccatgt cgacgtaatc cttgatcgcg
     7441 tgcaggtagc tccagggctg gcggaaggcg ctggcgttga gcggtcggta gtggggctga
     7501 tgcatgtgcc acatcagcac caccggcgtg aaggattcag cggacatgac gcacctcctg
     7561 cccgagcatc tcgggagaga ccagcacgat cccctcgggc gagaggtgga agcggcgggc
     7621 atcctccttg gcatcttccc cgatgacggt gccatcggga atgatgcact cctgatcgat
     7681 gatcgcccgg gtcagccggc agtgctggcc gatcacggcc cggggcagca ccacgcactg
     7741 gttcagttcg ctgtaggcgt tgacgcggac attgttgaac agcagcgagc ggcgcaccag
     7801 tgcaccggag acgatgcagc cggccgacac catcgaatcg accgccatgc cgcgtcggcc
     7861 atcgtcatcg tcgaagacga acttggaggg gggcagctgc tcctgatggg tccagatcgg
     7921 ccactcctgg tcgtagaggt tcagcggtgg ggtgacatcg agcagctcca tgttggcctg
     7981 ccagaacgca tcgaccgtgc cgacgtcgcg ccagtaggcc tgctcgccat tttcgacgtc
     8041 acggaacgga taggcgaaga cccggtagcg gtcgatggcc gaggggatga tgtccttgcc
     8101 gaagtcgtgg ctggagcggg gtgtctcggc atccttgatc agctgctcat agagaaaccc
     8161 ggtgttgaac acatagatgc ccatcgagac caatgccttg tcgggtgaac cgggcatcgg
     8221 gatcgggtgc ttcggtttct cctggaagcc gatgatgcgt tcgtcggcac cgatcgacat
     8281 gacgccgaat tcgcttgctt cggccagcga ggtttcgatg cagcagacgg tcatgtcggc
     8341 ctcgcgctcc acatgctggg cgatcagcag gccgtaatcc atcttgtaga tgtggtcacc
     8401 ggccaggatc agcacatatt cgggattgtg cagccggatg atgtcgagat tctgatagat
     8461 cgcatcggcg gtgcccttgt accaggagtc atggatgcgc tgctgggccg gcagcagctc
     8521 gatgaactcg ttcatctcgg cgcgcagaaa gctccagccc ttctgcacgt ggttgatcag
     8581 tgaatgtgat ttgtactggg tcaggatgcc gatgcgacgc acatcggagt tgacgcagtt
     8641 ggagagcgaa aagtcgatgg tgcggtactt gccgccaaaa gggatcgccg gcttggcatg
     8701 ccagcgggtc aggttgtgca gccgcgaacc gctgccgccg gccagcacca gcgccagggt
     8761 ctcaccggtg atgcggctga cgaaacggcg gggcgcggtc ggtgagctgc tgcgtttttt
     8821 gcccttggca gtcggtttgg tggtttccat gcggtcggct ccggggttca ttggcgttgg
     8881 cgattcagtg gccggtcgtg catccatcca aaggcgcctt ggccagagtt atgcagggct
     8941 tattccagtt ttgcaccgca ccgaggtggt gtcgtcatcc ggcagcgggt ttgatcgcac
     9001 tgcggcgtcg gtttttccgt ggtgcgcttt tctgcttcaa tgcactgctc cagcagttca
     9061 gttcagccag gtcgtcgaca tgactccttc gtcttcttct gtttcgccgc ccgtttccat
     9121 ggtgctcgat gaaacccagc ggcgcatcat cgaggcccgc catcacgatc ctttcggtta
     9181 tcttggctgc catccacggg gtgatctcaa ggtggtgcat gttcaccttc ctggtgcgcg
     9241 ttcggtccat attgtgacct caaacggtgc gcaagagcca ctggagcggc tggggcggag
     9301 cgacctgttc agttggcagg gtagtgccga cacattaccg aaacattacc tgttgcgctg
     9361 ggtcgaccgc tggggtgtcg agcatgaggc ccatgacccc tacagctttg gaccgcagct
     9421 gctggagttt gatctggcgc tgtttgccgc cggcaagcat tggcatgcct accgctttct
     9481 cggtgcacac tgtcgcgaga tcgatggcat cagcggggtg ttcttcgcgg tgtgggcgcc
     9541 caatgccgag cgggtcagcg tggttggcga tcacaatgcg tgggatggcc gccaccatcc
     9601 gctgcgctgt cgtggtgcat ccggggtgtg ggagctgttc attcccgggc tggctgccgg
     9661 cctgcactac aagttcgaga ttcgcgcccg caatggcgcc attctgctca agagcgatcc
     9721 ctatgcccag ggcagcgagt tgcgacccgc caccgccagc gtggtccgtg aaccaagccg
     9781 ccatcagtgg caggatgcac agtggctcga tcagcgacag cggcatgact ggctgcatgc
     9841 gccgctgtcg atctacgagg tgcatctcgg atcctggcgc cgtgacgagg cgggacactt
     9901 tctgaactac cgcgatctgg cccatggtct ggtcgattac atcgcgcagc agggctacac
     9961 ccatctgcaa ctgctgccga tcaccgaaca ccccttcgat ggctcatggg gctatcagag
    10021 caccggctac ttctgcccga cccgccgctt tggcgaaccg gacgagctgc gttacctggt
    10081 cgatctgtgc caccgtcatg gcatcggtgt gctgctcgac tgggtgccgg cgcactttcc
    10141 gcgcgatggc catgcactgg catggttcga tggcagcaac ctctatgaac atgccgatcc
    10201 acggcggggc gaacaccgtg actggggcac actgatcttc gactatggcc gcaacgaggt
    10261 gcgcaacttt ctgctcagca gcgccctgta ctggatcgag gagttccaca tcgacggcct
    10321 gcgggtcgat gcggtggcgt cgatgctcta cctcgactat tcacgccagc ctgatgactg
    10381 gctgcccaat atccatggcg gcaatgagaa cctcgaagcg atcgattttc tcaagcacct
    10441 caacggggtg gtccatgagc gtttccccgg cgtgctgatg atggccgagg agtcgaccgc
    10501 ctggccgatg gtgtcgcggc cggtccatct cggcggtctg ggcttcagca tgaagtggaa
    10561 catgggttgg atgaacgaca cgctcgacta catcggcaag gatccgatcc accgcctcta
    10621 ccaccacgac aagctcacct tcggcctgct ctacgccttc accgagaact tcatcctgcc
    10681 tttctcgcat gatgaagtgg tgcatggcaa acgttcgctg ctctacaaga tgccgggcga
    10741 tgagtggcag cggttcgcca gcctgcggct gctgctgacc tatcagttca cccagccggg
    10801 caagaagctc aacttcatga ccaatgacat tgcccagggg cgggagtgga accatgaccg
    10861 cgaactggag tggtacctgt tgcagtaccc ctttcatcag ggggtgcagc ggctggtggc
    10921 cgatctcaac cggctttacc gcaacgaacc ggcgctgcat cggcacgatt tcgacagcag
    10981 tggcttcgaa tggatcgact gccatgacag cacccagtcg gtgctgtcgt tcctgcgcaa
    11041 gagcgatcag ggctggttgc tggtgctgct caacttcacg ccggtggtgc gcgaagatta
    11101 ccgggtcggc gtgcccgctg ccggcagcta ccgggagctg ttcaactcca actcccgcca
    11161 ctatggcggc tccgacctcg gcaatggtca ggtggtgacc gagccggtcg cctggatggg
    11221 gcggccggtg tcgctgcggt tgaccttgcc gccactggct ggcatcgttc tgagactcgc
    11281 atgagaatca aggacccgta aagatggcca aggccaaggc acgcccgcgc aaaaaaccgc
    11341 ctgccgcgct cgatgtgctg tttgtcgcca gtgaagccca tccactgatc aagaccggcg
    11401 gtctggccga tgtcgccggc agcctgccga tcgccctgac gcagctgggg cagcggctcc
    11461 atctgctgct gccggcctac gccggggtca agcggcagag cggcgcactg ctcgagcggg
    11521 cccgcttcac gctggcccat ggccgctgcg gtgaggtcga agtcagactg ctggagggcc
    11581 ggctggccga cagcgaggtg atcctctggc tggtcgatgt gccggaatat ttcgcccgcg
    11641 agggtggccc ctatcaggat gccgctggcc aggattggcc ggacaatgcc gaacgttttg
    11701 cgctgttctg ccgggtggcg gcgctgctgg cgatcgatca gctcgggctg ggctggcggc
    11761 ccgacatcgt ccatctgaac gactggcaga gcgcactcac cgcgctgttt ctgcaaccct
    11821 tcccgtttcg tccgcggctg ctgttcacca tccacaatct ggcctatcag gggctgttca
    11881 gccgggcgca gttcaacctg ctcgacctgc cggagtcggc ctggtcgatc gagggggtgg
    11941 agtactacgg tcagctctcc ttcatcaagg ccggcatcaa ttgctctgac tggatcagta
    12001 ccgtcagccc cacctatgcg cgtgagatca ccaccttcgc ctatggctgc ggtctcgatg
    12061 gcctgctgcg ggcgcggcag ggtgtgctgg tcggcatcct caacggcatc gatgcgcagg
    12121 tgtggaatcc ggcgaccgat ccgctgattc cgcaccacta cagcgccgat gagctgagcg
    12181 gcaagcggct cgacaaggtc gctctgctgc ggcgcatggg tctgcccgcc gacgatgagc
    12241 tgccactgct ggcgatggtg acgcggttgg tcgagcagaa gggcatcgat ctgctgctgg
    12301 agatcctgcc ctggctgatg acgcagccgc tgcggctggt ggtgctcggc aatggcgacc
    12361 cgcactacac cgagctgctg ctgggttgcg cgcgccgcca tcccgaccgc ctggcagtgg
    12421 tggccggcta tgacgagccg ctggcgcacc agatcgaggc cggtgccgac ctcttcgtga
    12481 tgccgtcacg tttcgagccg tgcggactca accagctcta cagcctgcgc tatggcaccc
    12541 tgccgctggt gcggcgggtg ggtggccgac accgtcatcg atgccgatgc ggcaaccgag
    12601 ggcaacggct tctgtttcga cttcgccagc ggtccggcac tgcgtgccac gctggagcgg
    12661 gcgctggcgc tctatcatca accggcgcgc tggcaacaac tgatgcgctc ggcgatggcg
    12721 gccgatttct cctggcagcg cagctcggcc gactacctcg cgctctatcg gcggatgctc
    12781 gcgccgcagt gaggctcagg gtcgcggcag tgcatcctgc cgcgacccag tgtgcaggag
    12841 gctctcttca ttcgggcatc ttgctgtgct tttcccgcat cttctccatc cgctcgtgca
    12901 tcttctctct catcttgtcc cgttcgctct ggtcgatgta gccatcgtgg ttggcatcgg
    12961 ccttgtcgaa ccgcgcttcg gtcgatttgg tgaactcggc cttgctgatg cggttgtcgc
    13021 cgtcgctgtc catctgcttc cacatcatct gatggtcggg gccgccttcg tcaccgtggg
    13081 acatggccca gaccggcgcg gtcagtgcac agatcagtga ggtcgaaagc agcaggggtt
    13141 tgatacgcat ggtcttctcc ggtggtggat tgaagcgtcg atgggtgttg tcacccttgc
    13201 agcaggattg agccctgctg caagggcaat aacgggcgag ccggtgccgg gttgacgccg
    13261 ttgccgggcc tgctctggta aattgcccgc ttttttgcgg cggaggtctg gccttgatcg
    13321 actacgaaaa gctgtgggcg ccgctggcgc gtgctctgcc ggcggacggg tgtggcgcca
    13381 tcgagcaggc caccgcggca cagtggcacg ctgcgcgcca tggggatctg ccgcgctggc
    13441 aggcagcggt cgagaatctg ccagcggtgg agtcagcgca gctcggcatc gaccggggcg
    13501 ccgtcagcct gagcagcccg cagccgctgt cgcccgccga aaccgaacgg ctacgcagtg
    13561 gcctgctggc gctgcatccc tggcgcaagg ggccgttcaa tctgtttggc gtcgaggtcg
    13621 acagcgagtg gcgctgccaa ttgaagtggg cgcgcatcga gcgtgccatc gacccgctgg
    13681 tgggccgtcg tgtgctcgat gtcggctgcg gcaatggcta ctacagttgg cggctggcgc
    13741 tggccggtgc cgagctggtg gtcggcatcg atccggggct gctgttcacg ctgcaatatt
    13801 gggcggtggc gcgttacctt cctcggcgca ccgcctgggt gttgccgctg gcgctggagg
    13861 cgctacccga gggtgaggcg aactttgaca tcgtgctgtc gatgggggtg ctctaccacc
    13921 gccgctcgcc ggtcgagcat ctggagcggc tgcggcagct gctgcgtccc ggcgggcagt
    13981 tgctgctgga gactctggtg gtggagggtg atcggcagac cctgctgctg ccgccggggc
    14041 gctatgccaa gatgcgcaac gtctggttca ttcccagtgt cgcggcgctg ggcaacctgc
    14101 ttggacgctg cggttttcgt gatgtcgaac tggtcgatgt ctcgctgacc gatcacaacg
    14161 aacagcggcg caccgactgg atgcgcttcg aatcgctggc cgactttctc gaccctgccg
    14221 acaccacgct gaccatcgag ggccatccgc ggccacggcg tgcgctcttg gtggcgcgcc
    14281 gctgagcctc agtcgaggtg gcaggcgcgc cagtggtgcg gatcgaccgg gcgcgccacc
    14341 ggcggctcgc ggtggcagat cggcagcgcc tgtgggcagc gaccggcaaa gcggcagccg
    14401 gggggcagat cgatcggtga gggcatctcg ccctggatca gcggaagctg ccgtgcccgc
    14461 tcacgttgcg ggtcggcaat cgggttggat tcgatcagca gccggctgta ggggtggcgt
    14521 ggctggctga agagctgctc ggtcggcccc agctcgacca gttggcccag gtacatcacc
    14581 gcgacccggt cgcagaggtg gcgtaccatc gacaggtcat gggcgatgaa cagcagggtc
    14641 aggcccattt cggcgcgcag cgctgccagc aggttgacca cctgcgcctg caccgagaca
    14701 tcgagcgcgg agatcggctc gtcacagacc acgaaacggg ggcggctgat cagcgcgcgg
    14761 gcgatgccga tgcgctgccg ctggccgccg gagaactcat gcgggtagcg gcccatctgc
    14821 tcggccgaca gcccgacccg ttgcagccag tgcgccacct ggtcacgaat gcgaccacgg
    14881 tcgcgttcgc cctgcaggcg cagcccctcg ccgacgatct ccagcaccgt catgcgcgga
    14941 ttgagcgagg agtaggggtc ctggaagatc atccgcatcg cgctgccatg gcggcgaaaa
    15001 tcctcggtac ggtagcgcgc cggcagccgt tcgccgtcga accagacctc gccggcactc
    15061 ttctccagca gcccgatcag gcagcggccg agggtcgatt tgccggagcc actctcgccc
    15121 accagcccga ccacttcgtc cggttgcagc gtcagcgaca cccgatcgac cgccagcagg
    15181 cggcgctggc gttccagatc gaaccagcgg ctgaggtcgc ggacttcgag cagggccatc
    15241 agggtgatcc ttggtcgtcg cgcagcggct gatggagtgg cgcgacggca ggcgcctgcg
    15301 gatggtgcag ccagcagcgg gcgctgacat cggcgtcgag cggttgcagc ggcggctcct
    15361 cgcgctggca gatgcgcagt gcatgagggc agcgcgggtg gtagccgcag ccggtgggcg
    15421 gctggaacag gtcgggtggc gcaccggcaa tcgcccgcag tggcgcgcgg cttgccggat
    15481 cgggcatcgc ctgctgcaac cccagcgtgt aggggtgggc cgggcggcgg aagatcgact
    15541 cgacgctgcc gcgctcgacg atgcggccgg catacatcac cgccacctcg tcggccatgc
    15601 gggcgaccac gccgaggtca tgggtgatca ggatcatcgc catgccggtg cgggcctgca
    15661 acgcctgcaa cagccgcaga atctgcgcct gaatggtgac atcgagcgcg gtggtcggct
    15721 cgtcggcgat cagcagtgcc ggctggcagg cgatcgccat cgcgatcatc gcccgctgca
    15781 acatgccgcc ggagaactcg aaggggtagt tgcgggcgcg ccgctgcgga tcggggatgc
    15841 ggcactgctc cagcagctcg accgcctgac gcgtggcagc agccgggctc agaccacggt
    15901 gcagccgcag ggtttcggcg atctgatcgc ccacccgcat ggtcgggttc atcgaggtca
    15961 tcggatcctg aaacaccatg ccgatgcggg cgccgcgcag cgcgttggcg cggcgcggtg
    16021 caagggtgag cagatcctcg ccgaaaaagc gggccgaacc gcgcaggatg cgacccggcg
    16081 gcatcggcac cagccccatc agcgcctgca ccgtgaccga cttgccgcaa cccgactcgc
    16141 cgacgatcgc cagcgtcctg ccggcctcga cgccgaaatc gacgccgcgc accgcctgca
    16201 cccggccgcc atcgctggca aagtcgacgg cgagatcggt cacctcgagc agccgttcag
    16261 ccatcgctca ctcccggccg cgcagacggg cgtcgagcgc atcgcgcagg ccgtcgccca
    16321 gcaggttgaa ggccagcacg gtcaggctga tcaccagtgc cgggaacagc agctcgtgcg
    16381 gatgggtcag catggtcttg atgccatcgt tgcacatcga gccccaggat ggcgtcggcg
    16441 gtgccacgcc catgccgatg aacgacagaa aggcctcggt gaagatcgcc gagggcacgg
    16501 caaaggtcag cgacaccaga atcaccccca tggtgttggg cagcatgtgg cgcagcacca
    16561 gatagggggt tccagccccc atcagccgtg ctgcctgcac atagccctct tcgcgaatcc
    16621 gcaggatctg cccgcgcacc agccgcgcgg tgccgggcca gccgagcagc accatcgcca
    16681 gcagcagcgg cgcgatgccg ctctcgccgg ggccgatgcc gaagccgatg cggaacagga
    16741 tcatgaacag cagaaagggc agcgccacca cgaagtcggc aaagcgcatc agcaatgcat
    16801 cggtccggcc gcccagatag ccggccacgc cgccataaaa ggcaccgatc acgatcgaca
    16861 gcagcggcgc ggtcagaccg atgaagagcg agatgcgcgc gccatggatc agccgtgcca
    16921 gcaggtcgcg ccccaggtag tcggtgccga gcgggtgggg cgccagggtc acgctggcgc
    16981 cagccggcaa ttcatggccg cgccgccggg cctcatcgag cgtgagaccg agctgcacct
    17041 cgaccgccag ggttcgataa ccatcggcct cgtcgacgcc gtcggtggcc accacgctgt
    17101 agtagtggcg gccaggttgc gggttgagcc gatcctcatg gctcacctcg ctgccgccga
    17161 tgatgcgcac cagcggcagc cccaggtcgt cgccctccat cggcgggtgg tcggtgcggt
    17221 agaggatgta accgcgcgcc cccggtaccg cctgccactg caaccgcacc gactggctgg
    17281 tggggctgcc gaccacgctc aatgcactgc ttctgcccag ccgggccgca ggcagcgggg
    17341 cgcccacttc ggattcggcg gccggagtgc cgctccactc actggagagg tcgaccagca
    17401 gcgcgcgctc gccgagggtt ggtggccgtg acaactgctc cagatcctgt gcggccggat
    17461 cgaccggcca cagcagtggg ccggcgagcg cgaacagcag caggccgagc accagcccca
    17521 aggcggcaaa ggcgctgcgg ttctggcgca accgccgcca gccatcctgc cagtaggagc
    17581 gtgatgggcg caccgcgcgc tccatcgccg gctgtggcgg cagtggcaga aaatcgtcgg
    17641 cggcaagcgg accggccatc cgtcagccca cccggatccg tggatcgatg aagccgtaga
    17701 ggatgtcgat cgcaatcacc atgaagacca gaaaggcgcc atagaagacc gtggtgccca
    17761 tgatcaccgt gtagtcgagc tgctgcaccg cctgcacgaa gtagcggccg aggccgggaa
    17821 tcgcaaacac cagttcgatg acgaagccgc cggtggtgat cgcggccacc gccgggccga
    17881 gcaccgtgat caccggcagg atcgcattgc gcaactggtg gcgaaagaag atgcgcgcct
    17941 cgccgagccc cttggcgcgg gcggtgcgga tgtagtcggc atggatcacc tccagcatcg
    18001 acgagcgcat cagccgggtc agataggcca tggtgccaag ccccagcacc agcgccggca
    18061 cgacgaagtg gcgccattcg ccccagccgg ccaccggcag cagcgaccgg ccggccagcc
    18121 gattcagctc gacgatcgcc agttgcgcca gcgccgccac cacgaaaccc ggcaccgaga
    18181 tcgccacgat cacgccaagc atgatcagcg cgtcgggcca gcggccgcgg tagcgggcgg
    18241 tcagcgcgcc ccacaggatg ccgccggcgg cggcgatggc gagcgcgacc agtcccaggc
    18301 tggccgagac cggaaagtgc tcgcggatga tgtcgttgac cgcgcggttc ttctgggtga
    18361 acgagatgcc gaagtcaccg cgcaccatct gcccgagata gcgcaggtac tgcaccggca
    18421 gcggctggtc gagtccgtag tgctgttcga ggttggcgcg aatctccttc ggcaccgcct
    18481 tgtcgttcag cagtggatcg cccggcacca gatgcatcgc gaagaaggtg gcggtggcga
    18541 tgaaccagac ggtgatcacc ccttcgacca gacgtcgcag cagatagcgg tgcatggttc
    18601 agcccctgcg cctggcgagc gggtgaaccg gggtcggcag cgcggcttcg atcatggcgc
    18661 caccacccgt gcctgggtga agtcggggtc ggtgccgatc acccgccgca ccaccccctt
    18721 caactggcgg tgctgcaggt agacgctgcc gctctcatat tgcggaatga tcaccgcatc
    18781 ctcgatcacc agccgctgca gcgccgccat cgcctccatt cgcagccgtg gatcggtggc
    18841 atcgcgcgcc accgccaccc agtggtcgta gtccgggttg gcatagcggc cacggttgtt
    18901 gatgttccag gaagcaaaga ggtcgccgaa ggtcatgatg tcatcataat cggggcccca
    18961 gccggcggcc accagatcga actctccagc cagcatcttc gccagccgct gcttgaagat
    19021 ctgcttgtcg atcttcagct ccaaccccag ttgggtgcgc agcaggttct gcaggtactc
    19081 gccctgcttg gcggccgtcg gtgagtcgcc ggtcagatag accagtggcg gaaagcgctc
    19141 gagcccgagc tgctgtcggg ccagctccag ctcctgccgc gcctggttcg ggtcgattgt
    19201 caccctgggg gcgggaaact ccttgcgaaa actcccattc acccccttga tccacaccgg
    19261 aaacagcgac cgccccggca gcgtgccagg cacaccgacc acgcggttga ccagctcctg
    19321 cggatcggcc acccgctgga tcgcccgacg gaaatggcgg ttgccggtga tgcgcccggc
    19381 gcggtggttg aactccaggt agaacagcga cccggccgga aagatgcgaa tcttcacccg
    19441 ctgcctcagt gcgtcattca gctcctcggc actgaggccg accagcgcca ccttgttgtc
    19501 cttgaacagg ttgtaggccg ccgtcgaatc ggaggtgaca tagggcaggt cgatctcgtt
    19561 gagcacggtc tgcgcccggt tccagtactg ctcgttcttc accagcgtca gccgggcacc
    19621 gtgcacccag cgcgccagcc ggaacgggcc gttgtagagc agccgatcct gctccgcccc
    19681 gtagcgggtg ccctgggcgc ggtagaaatc ctcgcgaatc ggcaggtaga cgccaaaggc
    19741 ggtgagctgc agaaagtagg cggtcggctg ctccagatcg acctgcagcg tccgctcatc
    19801 gagcgcccgc acgccgagct gctccggcgg cagcctgccc tcgctgatcg ccttggcgtt
    19861 tttgatcgga tagaggatcg aggcatactc ggaggccatg gccggatcga ccacccgctg
    19921 ccaggcaaag acgaaatcat gcgcggtcac cggttggcca tcgctccagc gcgcgtcgtc
    19981 gcgcagccag aaggtggcgc tgcgttcatc cagcttccag tcgcgggcca cgccgccgac
    20041 cagttggccc tgcgcgtcgt agcgcagcag cccctcgtgg gtgtggccga gaatgaagaa
    20101 gctcacctgg tcggtcgcct tgatgctgtt cagcgacggc ggctcctgcc ccagcagcag
    20161 cgtgatcttc tgccgggcag catcgaccgc atcggccatc gccagcggcg ccagcagcgt
    20221 gcagcacagc agccagaggg gggccagcat ggccttgatc aacacccgcg ggggtggcaa
    20281 ctgctctctt ttcgacatca tgcgctcaca gcagaaccag cgcgcatatt gaacgatcct
    20341 cgggcgacag gccacagggc ggcccggcca atgttctgtc gcagccgttg tggagggcgc
    20401 aaacaaaaag gccggcgcca tggcgccggc cttttgggca aacctgagag gatcaggctt
    20461 tcttgcgtcg ggccatgccg atgccggcca gacccagacc catcagcgcc aggctggcgg
    20521 gctcgggcac gccctggacg ggtggtgtgc ctccaaaacc atccagatag acatacatcg
    20581 catgtgccga aggggtgcag tcacgcgcga tggcttccag cgtcagcgaa tcaccgacat
    20641 tcacgccgag tgcggcgagg tccagcccca ccacgttcca gccgagcggg tcactcgggt
    20701 tgctgttggc gctgtatttc cagccgccgg cagtgaccca ggcgccgcca tcggtttcat
    20761 agtgctgcac attgtaaatg gagctgctct gggtgttgtt gcgtaccgag acctgaaaga
    20821 acggcgtcga agtcgtggaa tggccagaga tctcttccac cgctgcccag gcaaagtaga
    20881 ggaaaccggc cgagccatcg gcttcggcgg ccaccgtggc cgtttggcga atgcggttgt
    20941 agatcgtgcc gccgccggaa tagccccagg ccagtgagtc acccgtacgc acgccgtagc
    21001 tgccgccgta ggtccggttg atgccgacga ttggatcggc gcccggagtc accagattcg
    21061 aagagagacc accattgcct gacgaggcaa aggaggtggc gccagcggtg gcggagacgt
    21121 agcttgccgt tccgccgtct gacgagaact gccagccatc caggttgcca ttttcgaaac
    21181 cgccattcgt gaacatcacg ccggccattg caggggctgc aatcagcatg gccgaaagcg
    21241 acagtgctgc ttttttgatt accatttttt tgctcctcag ttacttgact ctgggaatgg
    21301 atcccgccca agagaggcca cgatgtcatc aggtttgatt tgccaccgta ccgcactcag
    21361 cagtcatata agcactaacc gtgccacact gaataaaatg gttatttttc ttttttatta
    21421 caatatgttg tgttaaaaat ttcaagactc ataaggtttt ttgaggtggt gtcttaagca
    21481 aagtgtaaaa tatgttgaca ggcggtgggc tgttgcagcg caccacgccc gggtcgaatt
    21541 tcagcaggga gtgatccggg caaccctgcc tacaacccct tgcagtagac ctgcgagttg
    21601 cgctggtagt tgtagagcga gcgcttctca tccggcaggt cgtcgacggt ggccgggaca
    21661 aagccgcgct cgcggaacca gtgggcggtg cgggtggtga ggacgaagag ccgggcgatg
    21721 ccctgctggc gggcgcgctt ttcgatctgc tcgagcagca ggtcgccgcg ttcgccgctg
    21781 cggtagtcgg ggtggacgac gacgcaggcc agttcgccgc tgtggccatc actgaagggg
    21841 tagagcgcgg cgcagccgat gacgctgcca tcgcggtcga tgacgctgaa ctggccgatc
    21901 tcctcttcga gccgctcgcg cgagcggcgc agcagggtgc catcctcttc gagcgggcgg
    21961 atcagctcga tgatgccggc gacatcgtcg atcagggccg ggcgaatctt ctcgtaacgc
    22021 tcatggctga tcagcgtgcc ggcgccgtcg cgggtgaaga gttcgcgcag cagcgcgcca
    22081 tcctcgccaa agccgatgag gtggctgcgc tgcacgccgg cgctggaggc gtgcaggcag
    22141 ccacgcagcg ccgggctcga tgtcaggctg gcgtcatcga gccgctggcg aatctcgctc
    22201 ggcgtcagtt cgcgcagcaa ctggccgtcg cggtcgagca cgcccggttt ttcgctgtag
    22261 gcgatcagct tgtcggcgcg cagcgcgatg gcggtttcgg cggccacctg ctcgaacgag
    22321 aggttgaaga tttcgccggt cggcgagtag ccgagcggcg acagcagcac cacctcgccc
    22381 gctgccagat ggcggtgaat cgctgcatgg tcgatgccgc gcacctcgcc ggtgtggtga
    22441 aagtcgatgc catcatgcac gccatgcggc ttggcggtga cgaagttgcc gctgatgacg
    22501 ccgagccgca gccgctgctg cgatgactgc gacagcagcg cctcgatctc gctgcgcaga
    22561 atgccgaccg cctgcttcac cgcttcgagc agctcgaggg tggtgatgcg caggtggtca
    22621 tggaactggc tgcgcaggcc gcgctgttcg agcgccgcct cgatctgcgg tcgcgcgcca
    22681 tggacgatca ccagccgcac gccgaggctg ttcagcaggg cgatgtcctg cacgatgtgg
    22741 atgaagttgg cgtgggcgac ggtctcgccg ctcagcatca gcacgaaggt gcggccgcgg
    22801 tgggcattga tgtagggcga tgagtggcgg aaccacttta catagtcgat gtcgctgccg
    22861 ttcacggcct ctctcttcag cgggtggtgg cgggggccag acagagctgg ccgatcagat
    22921 cggtgagcag gtcgacgcag gggcggatgg catccagcgc caggaactca tcgggcttgt
    22981 gggcgcagtc gatctcgccg gggccgaaca ccaccgtctc cagccccagt tcggtcagaa
    23041 agggcgcctc ggtgccgaag gcgaccgcct cgctggcgtg gccggtgagc cgctccagca
    23101 gtcgcaccag cccggcgtcg gcggcggtct cgaaggcggg cagaccgggg aacagcggct
    23161 gaaagtcgat cggcatgccg agctgccggc tcagccggtc gagccgctcg ccgatcatct
    23221 gccgcacggt gtcgatctgc atgcctggca gcaggcgcag gtcgaactgc aactcgcagt
    23281 cgccgcagat gcggttgggg ttgtcgccgc catggatgca gccgaggttg agcgtcggcg
    23341 tcggcaccgc gaaggccgag ttgtggaact gcgcgcccag ctcatcgcgc agctcgagca
    23401 gcagcgtgat cacctgctgc atcccttcga tcgcattgcg ccccagcgcc ggatcggagg
    23461 agtggccggc ctgaccgacc acccgcaccg actccatggt gatgcccttg tgcagccgca
    23521 ccggccgcag gccagtcggt tcgccgatca ccgcgtggcg gccgcgcggc cgacccagcg
    23581 cgaccagttg gcgggcgccg cacatgctgc tctcttcgtc ggcggtggcg agaatgatca
    23641 gcggcgcgtt cagctgctgc ccggcaaact ggcgtgcggc ttcgatcgcc agcggaaaga
    23701 agcccttcat gtcgcagctg cccaggccat gcagacggcc ttcgcgctcg gtcagccgga
    23761 acggatcgga gcgccacagt tcagggtcgc agggcacggt gtcggtgtgg ccggccagca
    23821 ccaggccgcc gggaccgctg ccgagggtgg cgatcaggtt gcgcttggcg gcgccgccgg
    23881 gaccgcggcc ggcctcggtc acttcgatgg caaagccgag gcggtcgagc cagtcggcca
    23941 gcagctcgat caccgcgcca ttgccgaggt catgcgccgc gctggtggcg ctgaccgacg
    24001 gggtggcgac cagttgcgcg agctgttcgg tgagtgaggg caggtggcgc aggcgcatgg
    24061 tggattccgg ccgatggatg tcgccattct gcccagtttg gtgcgggcgg tccatgcgcc
    24121 gcggcgctac acttgcagca ggctcattgg ttaagctgcc cgctcatgcc ctcatcattg
    24181 caaggtcccg tgcccatgaa gtcgagtgaa tcgctgtcgc agtcggccga ccggaagctc
    24241 gatctgcgcc ggctgctcga agcattgcag gcgcagggtc ggctcagccg gtccgacttc
    24301 gagcaggcgc tcggcaaacc gcgcgatcag gctgccgtgg cgctgcatcc gctggtctgg
    24361 attgcccgcc agcagtatgg cgatgcgcag cagagtggcc ggacgctcga tctggagacc
    24421 ttgacgcagt ggctggcgca gcagattccc gaccagcagt actaccacat cgatccgctg
    24481 aagatcgatg tggcggccat caccgaggtg atgtcgtttg ccttctcgca gcggcaccag
    24541 attctggcgg tcgaggtcca gccgcaggag gtggtggtgg ccagcgccca gccgctggtg
    24601 cagggctggg agcccgacct cgcccatgtg ctgcgcaagc cgattcgccg ggtggtggcc
    24661 aaccccgaag agatcgagcg gctgtcggtc gagctctaca cgctggcgcg ctcggtcaac
    24721 agcgcctcgg cgcgcggcgc cgggccgagc catccgggcc ttggcaacct cgaacagctg
    24781 ctcgaaatcg gccaactgaa atcgcccgat gccaacgacc agcacatcgt caacatcgtc
    24841 gactggctgc tgcaatatgc cttcgaccag cgcgccagcg acatccacat cgagccgcgc
    24901 cgcgacgtcg gcaacctgcg ctttcgcatc gatggcgtgc tgcacaccgt ctatcaattc
    24961 cccagcgcgg tcaccgctgc cgtcaccagc cggctcaagg cgctcgggcg gctcaacgtc
    25021 gccgaaaaac gcaagccgca ggatggccgg ctgaagaccc gcgccccggc cggcaacgag
    25081 gtcgagctgc gcatctccac gctgccgacc gcctttggcg aaaagatggt gctgcgcatc
    25141 ttcgaccccg aagtgctgct gcgcagcttc gcccagcttg ggctcaccgc cgacgacttc
    25201 acccgctggg aggggatgat caaccagccg aacggcatcg tgctggtcac cggcccgacc
    25261 ggttcgggca agaccaccac cctctactcc agcctcaagc gcctcgccac cccccaggtc
    25321 aacctctgca ccatcgaaga tccgatcgag atgatcgagc ccaccttcaa ccagatgcag
    25381 gtgcagcaca acatcgacct cagcttcgcc tccggcgtcc gtgcgctgct gcggcaggat
    25441 ccggacatca tcatgatcgg cgagatccgt gacctcgaaa ccgccgagat ggcgatccag
    25501 gccgcgctga ccggccacct ggtgctgtcg acgctgcaca ccaacgacgc ccccaccgcc
    25561 gtcacccgcc tgctcgagct gggcgtgccg ccctacctgg tcaaggcgac gctgattggc
    25621 gtcatggccc agcggctggt gcggatactc tgtccgcact gcaaacgggc cgaaccgctc
    25681 gatctcgacg cctggcaggc actgatccac cccttcaagg tcaatccacc ggcgcggatg
    25741 tgcgtgccgg tcggctgtct ggagtgccgc gaaaccggtt ttctcggccg tgaaggcatc
    25801 tacgaaattc tgacgctgag cgatctggtc agagagcaga tcaccgccca gtgcgacctg
    25861 accgccctgc gccacatggg actgcgcgag gggatgcaca cgctgcgcct ctccggcgcc
    25921 cgcaaggtgg cggccggcct caccaccgtc gccgaggtgc tgcgggtcac gcccaacgag
    25981 atgcgcggct gagaccggga gcgcaggctg ctcctctgat cagcaaaggg ggtgttgggt
    26041 gcgtgcctga ttctggtggg cggaggaaaa cagttcgagt ctgagtccat ttttcctttc
    26101 cctttgcgat tcgtcattac tccggtacat ggccagcttc gcgctgatgc cggatggcaa
    26161 gaacaagaac gatgtcctgc tccacctcgt ggctgtataa cgcaaggtaa ccagacttgc
    26221 cgcgagagat gacaagctct cgcaacgcct gctccgcagg gcgtccgatc agtggatggt
    26281 ttgccaggac ttgcatcgct tcggtgatga gccccaccgt ctgtacagcg ctttctggat
    26341 cagcctcgac gagaaagtca gtgagtcgat caagatcagc cagcgctcgc gcggtgtaga
    26401 tcacgcgcgc caagagtggg ctcttggctt ggggagagac ttgccttgtg cgcgggcttg
    26461 cagataagca tgaacatcct cagatgcgta tccctcgcca gatttgatgg cttcttctcg
    26521 cgaagcgatt gcatcggtca cgaaccgtgc ccgcctttgc gcatttgcag cagcggtgcg
    26581 gattgcatca accatgaacg catgcggggt gatgccttgc tgcttggcgg cagcgacagc
    26641 aagctgcttt acatcatcag ggatttttaa tgacgtcgta gacatggccg ggcctcaggg
    26701 aaggtgtcac catggtaaca tcgcttggga aatcgcgaca gattggtccg gcgccactgc
    26761 ccgcagtgtg catcgtgcgg cgctgctgga gtggcccagt gcggcaaacg aaaaagcccg
    26821 caagcgtgaa ctgcttgcgg gcttttgacg tggttggcgg agcggacggg gctcgaaccc
    26881 gcgacccccg gcgtgacagg ccggtattct gaccaactga actaccgctc cggaacggtg
    26941 cgcgcttggt gggtggagag ggattcgaac ccccgacatt cgccttgtaa gggcgacgct
    27001 ctaccaactg agctatccac ccagcagcgc aagaagaggg gcattctacg gaaaatcacc
    27061 tctctgtcaa ttggagcttc ggctgttatg agcgaatatg agcgaagagg ctggtcggat
    27121 cggaaacgta gagggcagcc gggcgcgact gtataatcgc cgcccttttg ctgggctgga
    27181 cgatggagat ctacaaagag tttgttttcg aagcggcgca tcggctgccg gcggtgcctg
    27241 ccgggcacaa atgcgggcgg ctgcatggcc actcgttccg ggtgcgcgtc gtggtggagg
    27301 ggacgcccga tccagtcagc ggctggctga tcgattttgc cgagatcaag gcacgctttg
    27361 cgccgcttta tgcgcagctc gatcacaact atctgaatga gattccgggg ctggagaatc
    27421 cgaccagcga gcagttggcg atctggatct ggcagcggct gaagccgctg ctgccggcgc
    27481 tgtgtcgcat cgagctgcag gagacctgca cgtcgggctg catctacagc ggcgtctgat
    27541 tcagcgcggc tggggtctgg tttcgccggt gaggtcgtgg gtgaccggct ggatgccagt
    27601 ctgccgatag gcgcgaaacc gttgccgcga cagctcacgc tggagtggat cgctggtgac
    27661 gatttcggcc acgcggttga agccggcaaa gccgccgggc agtgcatcgg tcaggttgat
    27721 cagcaggtcg tcgcagcgat cgagccggat gcggtcgtcg aggaagatcc gctgcccctc
    27781 atcggccatc tgctcgccat gcggcagaaa gctctcggcc cgaaaactcc acagcagtgc
    27841 gtcgagcttt gcccgctgcg gttgatcgct gatcagcagc aggatggcgt ggccgaggcg
    27901 atgggctttc tccaccagac gacagacgaa caggtcgcgg gcatgcgggc tgccctcggc
    27961 cagcaggtag aagtcgacct gggtggacac cgtcagggct
//
LOCUS       DAORCQ010000026        27653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_955_length_27653_cov_5.425937, whole genome shotgun sequence.
ACCESSION   DAORCQ010000026 DAORCQ010000000
VERSION     DAORCQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 27653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27653
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_27653_cov_5.425937"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PKI23_04365"
     CDS             complement(<1..688)
                     /locus_tag="PKI23_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HNN36209.1"
                     /translation="MERDAALLLPTLTRIAEALERLTPRPPAAIDWSQTLAAHWLVGS
                     GGGVLAPLKTRTMRLDDLLGVERQKATLLANTRHFLAGLPANHALLWGSRGTGKSSLV
                     RALLHDCAGQGLRMVEVERSHLCDLPLIIAQLRDSTHRFIIYCDDLSFAEGDPAYKVL
                     KSVLDGSLDSASEQVLIYATSNRRHLLPEYMDDNRAARMVGELLHESESSEEKISLSD
                     RFGLWLSFYPF"
     gene            complement(688..1128)
                     /locus_tag="PKI23_04370"
     CDS             complement(688..1128)
                     /locus_tag="PKI23_04370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNN36210.1"
                     /translation="MNLPQIFAEARRTGDYGEVNELIPYAKLLGIRIEQPDAASLRFV
                     LPYQWDNVGNVKLPAIHGGVIAGLLENAATLHLLWAMESTTMPKPIDGNIDYLRSGRA
                     LETYAQCQVTKQGRRIANVAVKAWQEDADRPIAVARYHFKVTPS"
     gene            complement(1125..1628)
                     /locus_tag="PKI23_04375"
     CDS             complement(1125..1628)
                     /locus_tag="PKI23_04375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNN36211.1"
                     /translation="MSDSAQSDGQGRGYLIDDPRYYKATVEDSLTRIPHSAVLGLEIV
                     SIERDVAILRLPYSDRLIGNPVSRTVHGGAVLSLVDSASGKAVFCAVPAPETIATLDL
                     RIDYLKPPQPEQDLFCRAECYKLTRSVAFVRAEVYHQPGDPIAHSVSTFMRSSSSIAN
                     STMEPSR"
     gene            complement(1762..2115)
                     /locus_tag="PKI23_04380"
     CDS             complement(1762..2115)
                     /locus_tag="PKI23_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36212.1"
                     /translation="MEGVPMVYRFTGQFTLHRPLMDLLTPIDMSGLAIAILRSPQGPH
                     VLVSRIRGVQIDMAAQPGPDYHFSGQIEAERPVAESVLHRLSLAFTADRIAHRFELLN
                     DAGEPLQSFQGLPPQ"
     gene            complement(2115..2648)
                     /locus_tag="PKI23_04385"
     CDS             complement(2115..2648)
                     /locus_tag="PKI23_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36213.1"
                     /translation="MRSVHPMVGDVAAPDMAPLPCIIDVEASGFGAGSYPIEIGFVLP
                     TGRAHCYLLRPLPDWGHWDATAERIHRIDRATLLRHGRALPEVADALNHHLQGSKVYT
                     DAWGQDMAWLALLFDSAGVKQRFVLESIRALLSDAQAAAWSGMKQQAMVELRLDRHRA
                     SHDARIIQLAYRKSRVL"
     gene            complement(2648..3706)
                     /locus_tag="PKI23_04390"
     CDS             complement(2648..3706)
                     /locus_tag="PKI23_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNN36214.1"
                     /translation="MSSTSAAPAVGIIVNPMSGRDYRRLAARADTVSHEAKRNQIARA
                     VVGAIAAGARRILVPYEPRRLATGAVEQLDFAVEFERIETPILHKSIDTTRAVEAMRE
                     RGAAALLVLGGDGTHRVVTKAWREAPMVAVSTGTNNVFPTMQEPTLAGMAAGLVASGG
                     IALAECAERAKVVEVEIEGEAGDLALIDAVLLQNDSLGNLAPFASDKLRTIVLARAEP
                     ASVGISPLGGLLHPCGAEDEFGVTVNFCTHAQGGRLLRAPFSPGLIRTVHVADSRPVP
                     LGEPIEVTGPALLAFDGDRERQLAPGQRAWLRVVRSGPWVIDSRKALHLAAERELMLD
                     LDHWFDAYDGVAGKSGCC"
     gene            3893..5278
                     /gene="hemN"
                     /locus_tag="PKI23_04395"
     CDS             3893..5278
                     /gene="hemN"
                     /locus_tag="PKI23_04395"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNN36215.1"
                     /translation="MHSHELLFDPELINRYDRPGPRYTSYPTATQFHSGFTAADHSRH
                     ARNAAALTPALSLYVHLPYCSTICYYCACNKIVTKDRSRAQPYLDRLQRELAMQAELV
                     AGTQVEQLHWGGGTPTFITPAQISALMQAIDRHFTLSQAATREFSIEIDPREAPPATI
                     DLLRQLGFNRLSLGVQDFDPRVQQAVNRIQPEAITRRAIDAARTAGFDSLSIDLIYGL
                     PRQSVASFATTLDTLIDIAPDRVSLFNYAHMPELFKQQRGINALELPAPAEKLAILRL
                     AIERLTDAGYVYIGMDHFAKADNALTRAQQRGDLYRNFQGYSTHAHCNLLGLGISAIS
                     MLGSSYSQNCRSEAEYFAAIDQGRLPLFRGVELDADDLLRRAVITALICHFQLDIATI
                     ERDHRIDFSSYFNSEIKALKPMIEDELLELDARQITIKPRGKLLIRNICMVFDRYLAQ
                     QSVEQRFSRTI"
     gene            5430..7487
                     /locus_tag="PKI23_04400"
     CDS             5430..7487
                     /locus_tag="PKI23_04400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNN36216.1"
                     /translation="MNFEPKPTMLNAASVPELHGYRLGYRADIEGLRAVAVMLVVAAH
                     AGLPWLQGGFIGVDVFFVLSGYLITGLLVQEIDRTGRIALLDFYARRLRRLLPALLLM
                     LCGSSLLAILLLGPSEQPGQAKAAVAASLWLSNLHFNSVQLDYFGTGAESSLFLHTWS
                     LGVEEQFYLAWPLWVLFLLGSWSWQGGHRNWQRLRGGMLATMLLCLALSIFLTYVQPT
                     KGFYLMPSRAWQFALGAITLLTFSRTAGSAQSLTAPGSDFLARRVAGWLGLLLVLGSG
                     LFLDRHHAYPGGWALLPSIGTALILAAGTQTTAALGVSRLLSLPPLQRLGELSYSWYL
                     WHWPALLLGATVAIAGRPVHPMTWVILSLALAYLSHRWVESPLRHSRLIAARPLTTVL
                     GSAVLMGGTVLLGNLWQSKAVDWMGAPSQQPYVAVRSNLPALYAVPDCDEWYRSAQVR
                     VCAFGNAQGKQTVLLLGDSVTVQWFPAVMRTYAEQGWRVLVLTKSACPMVDEGYFYPR
                     IGRHYIECEQWRAAALAAANSLRPEVIVTGSTTTYPFTEAQWREGSRRTLGALSAAAE
                     QVFVLRATPALPFDGPDCLARHAWRSPLLPAPPACSAPASNSHDDAIFAWVSQATGAY
                     PNVRMLDLNALICPAQLCEAAREGRIVFRDTRHITASYIDSVGDAFAQRINETESHTA
                     AAH"
     gene            7696..8193
                     /locus_tag="PKI23_04405"
     CDS             7696..8193
                     /locus_tag="PKI23_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36217.1"
                     /translation="MRTVQDIATRHPQLSLIDAKRYTALLFGYRSWGSLAEETLRHDA
                     PPSPLDHELPKERLRTRVFNRIQRLRRETDLRPQPLLSIALSLQPRVKMVVKDMQKSL
                     WLRDFDELMEAFREVFPIRDNASIQDIFAAFDRYQLQLSAGRVDTSFEQSAVQSSAKS
                     SETQP"
     gene            complement(8230..8775)
                     /locus_tag="PKI23_04410"
     CDS             complement(8230..8775)
                     /locus_tag="PKI23_04410"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HNN36218.1"
                     /translation="MTPRQRIATAGGFTLIELLLVVGLLLTLLVMGVPSWRGSVERSE
                     VGKIQQDLQRDLALARSEAVRRGNSVTLRCANCSAGPPVVGWESGWTLFSDPNENQLL
                     DAGEELIRQHEAMPEATVRLSANPSNRLSYQASGTMAGIGGTFTVCPRRADVLQARGL
                     IYNSGGRVRVHGPNDTALSCP"
     gene            complement(8772..9731)
                     /gene="ispH"
                     /locus_tag="PKI23_04415"
                     /gene_synonym="lytB"
     CDS             complement(8772..9731)
                     /gene="ispH"
                     /locus_tag="PKI23_04415"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNN36219.1"
                     /translation="MKVLLANPRGFCAGVDRAIEIVNRALDLFGTPIYVRHEVVHNRH
                     VVDTLRERGAIFVEEVEEIPEGATAIFSAHGVSRAVRQAAEARRLRLFDATCPLVTKV
                     HMEVARLSQRGIDCVLIGHAGHPEVEGTMGQYDLTLGGAIHLVESLADVERLQVRDPA
                     RLAYATQTTLSLDDTARIVAALRARYPLIGGPRKEDICYATQNRQDAVRALAQEVDLL
                     LVIGSPNSSNSNRLQELAQRMGVAAYLIENAGALQPDWLRGCRKVGVTAGASAPDVLV
                     RQLIDRLLALGADSVEERAGRSEEVVFSLPRELKWQEAQGTPP"
     gene            complement(9728..10189)
                     /locus_tag="PKI23_04420"
     CDS             complement(9728..10189)
                     /locus_tag="PKI23_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNN36220.1"
                     /translation="MGSEAIGAGSRVRLCFSLATAEGELIDSNFGGPGAELVQGDGAL
                     PGYFETCLLGLVAGDRRSFQVAPEQGFGAVNPANRQWFNRDRFSADLTLEQGVVISFA
                     DPGGGELPGVVLAVEADRVCIDFNHPLAGMTLLFEVEILSVDNGQAEEGRQ"
     gene            complement(10179..10706)
                     /gene="lspA"
                     /locus_tag="PKI23_04425"
     CDS             complement(10179..10706)
                     /gene="lspA"
                     /locus_tag="PKI23_04425"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNN36221.1"
                     /translation="MPEARIQPADLLRMLCWLPLALLVVAIDQWSKAWIVANFALYQP
                     RPILSFFSLIHARNEGAAFSFLHDAGGWQQGFFIAIAGVISGAILLWLLRLPRSAHWQ
                     RIALLLILGGALGNLWDRLTLGYVIDFLLFHYEAHAFPVFNVADSAITAGATMLILET
                     LFCATPSSRAANHGQ"
     gene            complement(10699..13533)
                     /gene="ileS"
                     /locus_tag="PKI23_04430"
     CDS             complement(10699..13533)
                     /gene="ileS"
                     /locus_tag="PKI23_04430"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNN36222.1"
                     /translation="MSSYKETLNLPKTSFPMKANLAQREPQLLKQWQTQQLYRQIRQA
                     SAGRPRYLLHDGPPYANGEIHLGHAVNKVLKDIIVKAKGLSGFDAPYVPGWDCHGLPI
                     ELNVEKKIGKAGHKVSARAFRAACRDYASKQIDRQRQDFIRLGVLGDWDDPYLTMAPR
                     FEADIIRTLGRIIDRGHLLRGFKPVHWCIDCGSALAEAEVEYQEQRSPAIDVRFVVAE
                     EERFLGAFRELTEEPGRGPVSVVIWTTTPWTLPANQAVALNPRLDYLLVTASGPQGPE
                     RWVLAEPLVAAVMARAGIEHYRIIGRTPGSDLEGQWLLHPFLARQVPVILGEHVTAEA
                     GTGAVHTAPGHGLEDYQVGLKYGLEVLNPVDDRGCFKADTEFLAGLNVFAANEQVITL
                     LSERGRLLANRPLNHSYPHCWRHKTPVIFRATPQWFIAMQQQGLLDAAEAALEGVMFE
                     PAWGRARMSAMLNGRPDWCVSRQRAWGVPIPLFIHRTTGELHPRTAELIEQVALIVER
                     EGIDGWFELEPRTLLGDEADDYGKISDTLDVWFDSGVSWAAVLERRAELGLPADLYLE
                     GSDQHRGWFQSSLLAAVAATGAPPYRGLLTHGFTVDASGRKMSKSLGNVVAPQQVIQT
                     LGADVLRLWVAATDYRGEMTVSDEILNRTADSYRRIRNTARYLLANLDGFDPVQHRVP
                     VAEMVAIDRWVVRRALALQEELIAAYDSFQFHLIYQKLHNFCVTDLGGFYLDIIKDRK
                     YTTPADSLPQRSTQSAHYHVIEAMVRWLAPILSFTAEEIWQHLPGSRSPSVFLEQWYR
                     LPELERLQDPFSFDDWQRIMRVKEAVNRELERARSEGRIGGSLEAELRLHLAEPLRVH
                     LARLGDELRFVLITSAVELLPLSSDAAPVPAVESELPGLWIEVARSPHTKCVRCWHHR
                     PEVGTIAAHPELCGRCVDNVEGPGEARHHA"
     gene            complement(13582..14520)
                     /gene="ribF"
                     /locus_tag="PKI23_04435"
     CDS             complement(13582..14520)
                     /gene="ribF"
                     /locus_tag="PKI23_04435"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNN36223.1"
                     /translation="MELIRGMHNLRPRHRGCVATIGNFDGVHCGHQQILHALRLRAAE
                     LDLPSMVITFEPQPQEFFAGNQAPPRLTPFAHKMALLRQQGIDRVLCLRFNRHLSGMA
                     VDEFIDRLLVEGVGVRHLIVGDDFRFGHQRQGDYHALCTAGERFGFTVANTDTVVALQ
                     QRISSSRIRERLGEADLDEAELLLGRPYAFCGRVIEGQKLARQLGFPTANLSLRGRRP
                     ALNGVFAVEVELEGVQHPGVANVGMRPTVGGLQPLLEVHLLDYDGRLYGRRLTVTFRK
                     HLRAERKFADLAALTAAIAADVAAARHHFDTAMLRP"
     gene            complement(14591..16183)
                     /gene="murJ"
                     /locus_tag="PKI23_04440"
     CDS             complement(14591..16183)
                     /gene="murJ"
                     /locus_tag="PKI23_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNN36224.1"
                     /translation="MDQAERRSTALLKSSAVVALMTLLSRILGMVRDIVVARMVGSSA
                     GADAFFVAFKIPNFLRRLFAEGAFAQAFVPVLSEYRTQRSLEQTRELIDKTAGMLTLA
                     LMLVTLLAVVGAPLLTLLFAPGFQQDGAKMALTTEMLRITFPYLFFISLTAFCGSVLN
                     SFGRFAVPAVTPIFLNLTMIAAALWLAPRLDVPVHALAWGVLAAGLVQLLFQFPFLLR
                     LGLLPRPRLDWRHAGVRRIGRLMTPAIFGVSVSQINLLLDTILASFLETGSVSWLYYS
                     DRLVELPLGVIGIAIATVILPVLSRRAARQEIEAFSATLDWGLRGVLLIGLPATVALV
                     LLAEPLIFTFFQYGEMGLRDVEKSAFSLKFYALGLIAFMSIKVVATGYFARQDTRTPV
                     RIGIIAMVANMLLNLLLIWPLQHAGLALATALSGWLNAGLLLRGLLTGRIYRLATGWL
                     PFVLRIALANLLMGALLWWLSPEASVWAAWGVWQRVERVTLLCIGGFTGYLLALLLLG
                     EPLIRSTLSALLRRAVAARGGP"
     gene            16425..17306
                     /locus_tag="PKI23_04445"
     CDS             16425..17306
                     /locus_tag="PKI23_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNN36225.1"
                     /translation="MTISFEGKVAIVTGAGNGLGRQHALELARRGAKVVVNDLGGAVD
                     GSGGSSKAAEAVVAEIRAFGGTAIANGGSVSDKAGAKSMVDDAVKHFGTVHILINNAG
                     ILRDKSFSNMTFDDFEAVINVHLLGAVYVTKYCWEIMKEQKYGRILMTTSGSGLFGNF
                     GQTNYSAAKLGMVGFMNTLRLEGAKHNVYTNSLAPAALTRMTENLLPGEVAQGLAPEL
                     VTPGALFMVSDQAPNGVIIQAQGGSFGVAAILQNDKVSLGDHPTVEDVAANWERISDL
                     SKVGTRTEKLVSQPPKK"
     gene            17486..17731
                     /locus_tag="PKI23_04450"
     CDS             17486..17731
                     /locus_tag="PKI23_04450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNN36226.1"
                     /translation="MEVIHQAEHERFVVQVGEFEALLEYHLLPRQGVVNFHHTFVPPQ
                     IRGRGIAEKLVRTGLAWARTQQLKIEASCSYVRKFLR"
     gene            17827..18102
                     /gene="rpsT"
                     /locus_tag="PKI23_04455"
     CDS             17827..18102
                     /gene="rpsT"
                     /locus_tag="PKI23_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNN36227.1"
                     /translation="MPNIASAKKRTRQAEKNRQNNVSLRSKVRTYIKTAANQIEAGDV
                     EKAQQAFQRAVPIIDSMVNKGIIHKNKAARHKSRLSAKIKALALAAK"
     gene            complement(18179..19285)
                     /gene="proB"
                     /locus_tag="PKI23_04460"
     CDS             complement(18179..19285)
                     /gene="proB"
                     /locus_tag="PKI23_04460"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNN36228.1"
                     /translation="MRSTRRWVIKIGSALLTANGRGLDTEAMSAWVAQVVELRRRGVE
                     VVLVSSGAVAEGMLRLGLKQRPQLIHELQAAAAVGQMGLVQAYETYFHRFGLHTAQIL
                     LTHDDLSDRKRYLNARATLKELLRLGVIPVVNENDTVVTDEIRFGDNDTLGALVTNLV
                     GAEMLLILTDQDALYQADPRRQPDAEVIHGALATDPHLLEIAGGSGTALGRGGMVTKV
                     QAARLAARSGAMTLIAGGRLPHVILRLHEGEPLGTLLLPEQDPLAARKQWLAAHLQTR
                     GDITIDAGAVRMLRECGKSLLPVGVKAISGTFSRGEMVVCSDEAGRSVARGLVNYGHE
                     EAVKLIGQPSGRIAELIGYMGEPELIHRDNLVLL"
     gene            complement(19344..20525)
                     /gene="obgE"
                     /locus_tag="PKI23_04465"
     CDS             complement(19344..20525)
                     /gene="obgE"
                     /locus_tag="PKI23_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNN36229.1"
                     /translation="MKFVDEATIWVEAGNGGNGCLSFRREKYIERGGPDGGDGGAGGS
                     IYLEADANCNTLVDFRYQPRYRAGHGENGRGANCTGHNGADLILKVPVGTTVIDVDSE
                     QPIGDLVQAGDRLLVAAGGHRGLGNTRFKSSVNRAPRKTTSGGAGERRQLRLELKLMA
                     DVGLLGLPNAGKSTFIRAVSAARPKVADYPFTTLIPNLGVVSIEPHRSFVVADVPGLI
                     EGASQGAGLGFRFLRHLTRTRLLLHLVDVAPLDHSDPVDAARVIIDELEQFSPTLAAR
                     PRWLVLNKIDLLPIEEVEQLVQRMRAELPWEGPLHLISALAHQGTELLCQQLQEYLDQ
                     VNQEEAEDSEARQRELETRRRMEEEARKRVRDWRASQQLLPDDEDEDEDDEEAEVFYV
                     R"
     gene            complement(20749..21006)
                     /gene="rpmA"
                     /locus_tag="PKI23_04470"
     CDS             complement(20749..21006)
                     /gene="rpmA"
                     /locus_tag="PKI23_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNN36230.1"
                     /translation="MAHKKAGGSTRNGRDSESKRLGVKLYGGQLAKAGSIIVRQRGTA
                     FHPGANVGCGTDHTLFAKAEGRVQFEVKGPKQRRYVNIVPV"
     gene            complement(21046..21369)
                     /gene="rplU"
                     /locus_tag="PKI23_04475"
     CDS             complement(21046..21369)
                     /gene="rplU"
                     /locus_tag="PKI23_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNN36231.1"
                     /translation="MYVVFESGGKQHRAKLGDRIRLEKIEQAPGESVEFDRVLLVGEG
                     SDVKVGAPYLTDSNITAEVIGHGRLKKIKIIKFRRRKHHMKQAGHRQWYTEVKIMAIG
                     AVVKA"
     gene            complement(21600..22307)
                     /gene="pyrF"
                     /locus_tag="PKI23_04480"
     CDS             complement(21600..22307)
                     /gene="pyrF"
                     /locus_tag="PKI23_04480"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNN36232.1"
                     /translation="MSLPSGPRTILALDYHDPQAALRLVEMLDPQLCRLKVGKELFTR
                     SGPEFVCKLIEQGYEVFLDLKFHDIPQTVARAVQAAAELGVWMVNVHASGGRHMMESA
                     ANALARYPRAPLLIAVTVLTSLDRYDLNEIGVAHTPADWAVTLAKLAEDSGLQGVVCS
                     AQDLAMLKPEVDAAFLRVTPGIRLPGDEQHDQKRVLDPAAAIRAGSHYLVVGRSVTAA
                     ADPVAKLHVINAQISAA"
     gene            complement(22304..23485)
                     /locus_tag="PKI23_04485"
     CDS             complement(22304..23485)
                     /locus_tag="PKI23_04485"
                     /inference="COORDINATES: protein motif:HMM:NF037181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36233.1"
                     /translation="MLLGWLFGRRSMTLRLRTEPAASQARYLSDLDRLITVEKSSFVN
                     ALSKILDEDQSGVEFYLVIAALLRRRGEVEYAIECHQTLLNRFAVDSPLHGELIFELA
                     KDYIHAGLHDRAELLLNDLVERYPVLRIAAMEALLELHQGSREWHEAIAVGARMRTLL
                     RSPALLLDAKNQQRLVALHCALAHYHCELAEAGLATADHSAVRTALAAALKLDRKSVR
                     ASWLLTALELELSNQEAALRHLRRVRQQAPEWLADTVVLFLDRIQRLAPVAMVEQWML
                     NWLDEYPSVAMIMVTTEFQLQVHGPRRTYDFLLLQLHRFPSFRPLLDLLERHFQPEVT
                     DLAMLFRSLKQLSDQFMEEKSKYLCSHCGFKGTKVHWQCPSCREWNSIKPIFGQNLEY
                     R"
     gene            complement(23524..23883)
                     /locus_tag="PKI23_04490"
     CDS             complement(23524..23883)
                     /locus_tag="PKI23_04490"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNN36234.1"
                     /translation="MWRKRIQQIVSPDIFKRTMRRLKHSIQWIAVLLLMSMTAWFMTE
                     NSAPVQPQLWSLQLPQLSLGAWLVLAFFAGACAASLVTSVALARWRLRLSSVERQNAR
                     EKERAATPAVDASPSHR"
     gene            complement(23942..24229)
                     /gene="ihfB"
                     /locus_tag="PKI23_04495"
     CDS             complement(23942..24229)
                     /gene="ihfB"
                     /locus_tag="PKI23_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNN36235.1"
                     /translation="MTRSELIERIAAKQPQLPLKDVELAVRCILEQMSQVLATGKRIE
                     VRGFGSFCLHFRQPRVARNPKTGESVPLQGKHVPHFKPGKELRDRVNDLIE"
     gene            complement(24335..26014)
                     /gene="rpsA"
                     /locus_tag="PKI23_04500"
     CDS             complement(24335..26014)
                     /gene="rpsA"
                     /locus_tag="PKI23_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNN36236.1"
                     /translation="MAESFSELFEQSLKEINMQPGSIVSGTVVDIDSDWITVHAGLKS
                     EGVIPKNQFLNENGELTIQIGDTVKVALEAIEDGYGETRLSREKAKRVEVWQDLEQAF
                     TDETLVKGLINGKVKGGYTVDIQTIRAFLPGSLVDVRPVRDAVDLEGKTLEFKIIKLD
                     QKRNNVVVSRRAVLEQATSAEREALLVSLQEGMEVPGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRIKHPSEIVNVGDEITVKILKFDRERNRVSLGLKQLGEDPWLSIIQRYPV
                     GSRVMGKVTNLADYGCFVEIEEGVEGLVHISEMDWTNKNIHPSKVVQIGDEVEVQVLD
                     IDEERRRISLGIKQCKQNPWEKFSESYNRGDRISGKIKSITDFGIFIGLEGNIDGLVH
                     LSDISWDEAGEEAVRHYKKGDDIETVILSIDPERERISLGVKQLLSDPFNDYADAHDK
                     GSVVTGTVQEVEAKGAVVQLAEGVEAALKASEASSDRIDDLRQLLKPGDVIEAKIVNL
                     DRKTRAIALSIRAKDAVDQKQALKELRKQEAEAVSPTTIGDLIKAQMENQG"
     gene            complement(26158..26856)
                     /gene="cmk"
                     /locus_tag="PKI23_04505"
     CDS             complement(26158..26856)
                     /gene="cmk"
                     /locus_tag="PKI23_04505"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNN36237.1"
                     /translation="MGRVEAEPPPVITLDGPSGVGKGTVALHLARQSGWHLLDSGALY
                     RLVALIAQRQEIPLSAEARLAEAAATMDVRFDIDPLGGCTQVRLDGQVVDAALRSEEC
                     GNRASQVAVHPQVRAALLDFQHGFQRAPGLIADGRDMGTVVFPAAALKIYLTASAAVR
                     AQRRRTQLKEQGLDVSLTRLIEEIEARDQRDASRSVAPLRPADDALVIDTTDLGIDEV
                     VARVWQEAKNRRLL"
     gene            complement(26849..>27653)
                     /locus_tag="PKI23_04510"
     CDS             complement(26849..>27653)
                     /locus_tag="PKI23_04510"
                     /EC_number="1.3.1.12"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075022.1"
                     /note="catalyzes the formation of 4-hydroxyphenylpyruvate
                     from prephenate and the formation of tyrosine from
                     arogenate and catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional prephenate
                     dehydrogenase/3-phosphoshikimate
                     1-carboxyvinyltransferase"
                     /protein_id="HNN36238.1"
                     /translation="LLLAGLYAQGETAVIEPAPTRDHTERMLRSLGYRVESHPVAAGQ
                     RISLQGGGTLRAIDIQVPGDISSAAFFIVGATIAPGSNLLLRNVGINPTRTGVIDILR
                     QMGADISLERQYSAGDEPVADLRIRHAPLRGIEIPEALVPLAIDEFPAILVAAAQATG
                     ETVLSGASELRVKESDRIQAMADGLKVLGVDSVVRPDGIVLRGGAFGGGRIDSHGDHR
                     IAMAFAMAALRASAPLQITDCANVATSFPNFAASAQQAGLGIRVENADG"
BASE COUNT         5304 a         9278 c         8245 g         4826 t
ORIGIN      
        1 cgaaggggta gaacgacagc cagagcccaa agcgatctga aagcgagatc ttctcttcgc
       61 tgctctcgct ctcgtgcagc agttcaccaa ccatgcgcgc cgcacggttg tcatccatgt
      121 attcaggcag cagatggcgc cggttggagg tggcatagat cagcacctgc tcgctggcac
      181 tgtcgagcga gccatccagc acactcttca gcaccttgta ggccggatca ccttcggcaa
      241 aggagaggtc gtcgcaatag atgatgaagc ggtgcgtgct gtcgcgcagt tgcgcgatga
      301 tcagcggcaa atcgcacagg tggctcctct ccacctcgac catgcgcagg ccctgcccgg
      361 cgcagtcgtg cagcagcgcc cgcaccagcg aggatttgcc ggtgccgcgc gacccccaca
      421 gcagcgcatg gttggctggc agaccggcca gaaagtggcg ggtgttggcc agcagcgtcg
      481 ccttctgccg ctccaccccc aacaggtcgt cgagtcgcat cgtccgtgtc ttgagcgggg
      541 ccagcacacc accgccactg ccaaccagcc agtgcgcggc cagggtctgg ctccagtcga
      601 ttgcggccgg cgggcgcggg gtcagccgct ccagcgcctc ggcgatgcgg gtcagcgtcg
      661 gcagcagcag tgcggcatct ctttccatca gcttggcgtc accttgaagt ggtagcgggc
      721 caccgcaatc ggccggtcgg catcctcctg ccacgccttg accgcgacat tggcgatgcg
      781 tcgcccctgc ttggtgacct ggcactgggc gtaggtttcg agcgcgcgcc cggagcgcag
      841 gtagtcgatg ttgccatcga tcggcttggg catggtggtc gactccatcg cccacagcag
      901 atgcagggtc gcggcattct ccagcaggcc ggcgatgacc ccgccatgga tcgccggcag
      961 cttgacgttg ccgacgttgt cccactggta aggcagcacg aagcgcagcg atgccgcgtc
     1021 gggctgctcg atgcgaatgc ccagcagttt ggcatagggg atcagttcat tgacctcgcc
     1081 atagtcgcca gtccggcgtg cctcggcaaa aatctgcggc agattcatcg gctcggctcc
     1141 atcgtcgagt tggcgatact gctggatgag cgcatgaagg tggagacgct gtgcgcgatc
     1201 ggatcacccg gctggtggta gacctcggcc cgcacgaagg ccacgctgcg ggtcagcttg
     1261 tagcactcgg cacggcagaa cagatcctgc tccggctgtg gtggcttcag gtaatcgatg
     1321 cgcaggtcga gggtggcgat ggtctcgggc gcgggcaccg cacagaagac cgccttgccg
     1381 ctggcactgt cgaccagtga cagcaccgca ccaccatgca cggtgcggct caccggattg
     1441 ccgatcagcc ggtcgctgta gggcagccgc aaaatcgcca catcacgctc gatcgagacg
     1501 atctccagcc ccaataccgc cgaatgggga atgcgagtca gcgaatcctc caccgtcgcc
     1561 ttgtagtagc gcggatcatc gatcagataa cccctgccct gcccgtcgct ctgcgcactg
     1621 tcagacatcc accctttacc tcttttgctc ggtgaagcgc ctgatccaga actgctggcc
     1681 ggcgcgtgac aagcgcgcaa ttctaacccg gattgcctag gtgctccgcg tgaaaacagc
     1741 gcctgctcga tcggtgagcg gctattgggg cggcaacccc tgaaagcttt gcaacggctc
     1801 gcccgcatcg ttcagcaatt cgaagcggtg ggcgatgcgg tcggcggtga aggcgagcga
     1861 cagccgatgc agcaccgact cggccaccgg ccgctctgcc tcgatctgac ccgagaagtg
     1921 gtaatcaggc ccgggctgtg ccgccatgtc gatctgtact ccacggatgc gcgacaccag
     1981 cacatgcggc ccctgcggcg agcgcagaat ggcaatcgcc agaccactca tgtcgatcgg
     2041 cgtcagcaga tccatcaacg gccggtgcag ggtgaactga ccggtgaaac gatagaccat
     2101 cggaaccccc tccatcacag cactctgctc ttgcggtagg ccaactggat gatgcgtgca
     2161 tcatggctcg cccggtggcg gtcgagccgc agctcgacca tcgcctgctg cttcatgcca
     2221 gaccaggccg ccgcctgggc atcgctcagc aacgcacgaa tcgactccag cacaaagcgc
     2281 tgcttcaccc cggcactgtc gaacagcagt gccagccagg ccatgtcctg cccccaggca
     2341 tcggtgtaga ccttgctgcc ctgcaaatgg tggttcaacg catcggccac ctcgggcaac
     2401 gcccgcccgt ggcgcagcag cgtcgcgcgg tcgatgcggt gaatccgttc ggcagtggca
     2461 tcccagtggc cccaatcagg cagtggccgc agcagatagc agtgggcccg gcctgtgggc
     2521 aggacgaatc caatttcgat cggatagctg ccggcaccaa aaccggacgc ctcgacatcg
     2581 atgatgcagg gcagcggtgc catgtccggc gccgcgacat ctccgaccat cggatgcacc
     2641 gatctcatca gcagcagccg ctcttgccgg caacgccatc ataggcatcg aaccagtggt
     2701 cgagatcgag catcagctcg cgctcggccg ccagatgcag cgctttgcga ctgtcgatca
     2761 cccaggggcc actgcgcacc acccgcaacc aggcgcgctg gcccggcgcc agctgccgtt
     2821 cgcggtcacc gtcgaaagcc agcagcgccg gcccggtcac ctcgatcggc tcgcccagcg
     2881 gcaccgggcg cgaatcggcg acatggacgg tgcggatcag tccgggcgag aagggggcac
     2941 gcagcaatcg cccgccctgc gcatgggtgc agaagttgac cgtcacgccg aactcatcct
     3001 ccgcgccaca ggggtgcagc agcccgccga gcggcgagat gcccaccgag gccggttcgg
     3061 cccgcgccag cacgatggtg cgcagcttgt cgctggcgaa gggggcgaga ttgccgagtg
     3121 agtcgttctg cagcagcacc gcgtcgatca gcgccagatc gccggcctcg ccctcgatct
     3181 cgacctcgac caccttggcg cgctcggcac actccgccag cgcgatgccg cccgaggcga
     3241 ccagtccggc ggccatgccg gccagtgtcg gctcctgcat ggtgggaaag acgttgttgg
     3301 tgccggtcga gaccgccacc atcggcgctt cgcgccacgc cttggtcacc acccggtgcg
     3361 tgccatcgcc acccagtacc agcagtgccg ccgcaccgcg ctcgcgcatc gcctcgacgg
     3421 cgcgcgtggt gtcgatcgac ttgtgcagaa tcggcgtctc gatgcgctcg aactccacgg
     3481 cgaaatcgag ctgctccacc gcaccggtgg ccagccgcct cggctcatag ggcaccagaa
     3541 tgcggcgcgc accggccgcg atcgccccca ccaccgcccg ggcgatctgg ttgcgcttgg
     3601 cctcgtggct gacggtgtcg gcccgggcgg ccagccggcg gtaatcccgc cccgacatcg
     3661 gattgacgat gatgccgaca gcgggtgcgg cggatgttga actcatgcgg agaatcctga
     3721 acaggggttg cgggcatttc ccagccgaag ctgggcatga cggcaccatg ataaaagatt
     3781 gatcgcgaca ctccaccccg ggtgatggcg actgttgaca tggatcatcc agcagaagcg
     3841 ggctttgcgg taggctgcac gttgtattca ccgccacgac cggataccca cgatgcactc
     3901 gcacgaactg ctgttcgacc cggaactgat caaccgctac gaccggcccg gaccgcgtta
     3961 cacctcctac cccaccgcga cccagttcca cagtggtttc accgcggccg accacagccg
     4021 tcacgcacgc aacgccgcgg cgctgacccc ggcgctgtcg ctctatgtcc acctgcccta
     4081 ctgcagcacc atctgctact actgcgcctg caacaagatc gtcaccaagg accgcagccg
     4141 cgcccaaccc tacctcgacc ggctccagcg cgaattggcg atgcaggccg aactggtggc
     4201 cggcacccag gtcgagcagc tccattgggg cggcggcaca cccaccttca tcacgccggc
     4261 gcagatcagc gcgctgatgc aggcgatcga tcgccacttc acgctgagcc aggcggcgac
     4321 ccgggagttc tcgatcgaga tcgatccgcg cgaggcgccg cctgccacca ttgatctgct
     4381 gcgccagctc ggcttcaacc gcctgagcct cggcgtgcag gatttcgacc cgcgggtgca
     4441 gcaggcggtc aaccgcatcc agcccgaggc gatcacccgt cgcgccatcg acgccgcccg
     4501 caccgccggc ttcgactcgc tcagcatcga cctgatctac ggcctgccgc gccagagcgt
     4561 cgcctccttc gccaccaccc tcgacaccct gatcgacatc gcgcccgacc gggtgtcgct
     4621 gttcaactac gcccacatgc cggagctgtt caagcagcag cgcggcatca acgcgctgga
     4681 gctgccagcg ccagccgaga agctggccat cctgcgactc gccatcgagc ggctgaccga
     4741 tgccggctat gtctacatcg gcatggacca cttcgccaag gccgacaacg cactgacccg
     4801 ggcgcagcag cgcggcgacc tctaccgcaa ctttcagggc tactcgaccc atgcccactg
     4861 caatctgctg ggcctcggca tcagcgcgat cagcatgctc ggcagcagct acagccagaa
     4921 ctgccgcagc gaagccgagt actttgccgc gatcgatcag ggccggttgc cgctctttcg
     4981 cggcgtcgaa ctcgatgcgg acgacctgct gcgccgcgcg gtcatcaccg ccctgatctg
     5041 ccacttccag ctcgacatcg ccaccatcga gcgcgaccac cgcatcgact tcagcagcta
     5101 tttcaacagc gagatcaagg cactgaagcc gatgatcgag gatgagctgc tggagctcga
     5161 tgcgcgacaa atcaccatca aaccgcgcgg caagttgctg attcgcaaca tctgtatggt
     5221 gttcgaccgc tatctggcgc agcagagcgt cgagcagcgc ttctcccgca ccatttgagg
     5281 ggtgcgctgc gacagcggca aggctgcacc gtcttcgccc acggctgcta tcctttgctg
     5341 catcctgcct ctcgattcgg ctcgatgggg ctttttgacg cagccggcca gcggcctgat
     5401 cgactgatgg cgatcggcag ggagcgccct tgaacttcga acccaagccc accatgctga
     5461 atgcagcctc cgtcccggag ctccacggtt accgccttgg ctaccgtgcc gacatcgagg
     5521 ggttgcgcgc ggtcgcggtg atgctggtgg tggccgcgca tgccggcctg ccctggctgc
     5581 agggcggctt catcggcgtg gatgtcttct tcgtgctctc gggttacctg atcaccggtc
     5641 tgctggtgca ggagatcgac cgcactggcc gaatcgctct gctcgatttc tatgcgcggc
     5701 gcctgcgccg cctgctgccc gcactgctgc tcatgctgtg cggcagcagt ctgctggcca
     5761 tcctgctgct gggcccttcc gagcagccgg gacaagccaa ggcggcagtg gccgcctcgc
     5821 tctggctcag caacctgcac ttcaactcgg tccagctcga ctatttcggc accggcgccg
     5881 agagcagcct ctttctgcac acctggtcat tgggggtcga ggagcagttc tatctggcat
     5941 ggcctctgtg ggtgctgttt ctgctcggca gctggtcatg gcagggcggt catcgcaact
     6001 ggcagcggct gcgtggcggc atgctggcca cgatgctgct ctgcctggcg ctgtcgatct
     6061 ttctgaccta tgtgcagccg accaagggct tttatctgat gccctcgcgg gcctggcagt
     6121 tcgcgctggg tgcaatcacc ctgctcacct tctccagaac ggcaggctca gcacagagcc
     6181 tgactgcacc tggatcggac tttcttgcgc gcagggtggc cggctggctc ggtctgctgc
     6241 tggtgctggg cagcggactg ttcctcgacc ggcaccacgc ctaccccggc ggctgggccc
     6301 tgctgccatc gatcggaacc gcactgatac tggccgccgg cacccagacc accgcggccc
     6361 tcggcgtgag cagactgctg tcgctgccgc cgctgcaacg gcttggagag ctctcctact
     6421 cctggtatct gtggcattgg ccggcgctgc tgctcggcgc caccgtggcc atcgccgggc
     6481 ggccggtgca ccccatgacc tgggtgatac tcagtctcgc actggcctac ctcagccatc
     6541 gctgggtgga atccccactg cggcacagca ggctcatcgc cgctcgaccg ctgaccaccg
     6601 tgctgggctc ggccgtgctg atgggcggca cggtgctgct gggaaacctg tggcaaagca
     6661 aggcggtcga ctggatgggc gcaccgtcgc agcagcccta cgtcgccgtg cgttccaacc
     6721 tgccagcgct ctatgcagtg cccgactgcg acgagtggta ccgctctgcc caggtgcgtg
     6781 tctgcgcatt cgggaatgcg cagggcaaac agaccgtgct gctgctcggc gacagcgtga
     6841 cggtgcagtg gttccccgcc gtcatgcgca cctatgccga gcaaggctgg cgcgtcctgg
     6901 tcctcaccaa atcggcctgc ccgatggtcg atgaaggcta cttctatcca cgcatcggca
     6961 ggcactacat cgagtgcgaa cagtggcgcg cagccgcact ggcagcggcg aacagcctgc
     7021 gccccgaggt aatcgtgacc ggatcgacca cgacctaccc cttcaccgaa gcccaatgga
     7081 gagagggctc gcgacgcacg ctgggcgcgc tgtcggccgc tgccgaacag gtgttcgttc
     7141 tgcgcgccac acctgccctg ccatttgacg gtccagactg tctggcccgc catgcctggc
     7201 gcagtccgct gctgcctgcc ccgccggcgt gcagtgcacc ggccagcaac agccacgacg
     7261 acgccatctt cgcctgggtc agccaggcca caggcgccta cccgaatgta cggatgctcg
     7321 acctcaatgc cctgatctgt ccagcgcaac tgtgcgaggc ggcacgggaa ggacggatcg
     7381 tgtttcgcga tacaaggcac atcaccgcca gttacatcga cagtgtcggt gacgcctttg
     7441 cacagcggat caacgaaacc gaatcccaca ctgcggcggc acattgatcc gaactgcccg
     7501 atcgcggtca ccacgcgtct gccgggtcac ccgctgccaa cagagcgctg ccgaatttct
     7561 gctgtcgccc cttccacaac cacccggctg cgcactacac tgcgagcgtc atcacgccta
     7621 cttcaccggg ggatccccat ggaacagcgc cgatgtgagc aggagaaggt ccatttcgac
     7681 tcgatggacc atccgatgag aacagtgcag gacatcgcca cccgccatcc acagctgagc
     7741 ctgattgacg ccaagcgtta cacggcgctg ctgtttggct accgctcctg gggatcgctg
     7801 gccgaagaga cgctgcgcca cgacgccccg ccttctcccc ttgatcatga gttgccgaag
     7861 gagcgcctgc gaacccgcgt cttcaaccgc atccagcggc tgcggcgcga gaccgatctg
     7921 cgacctcagc cactgttgag catcgcgctc agcctgcagc cacgggtgaa gatggtggtg
     7981 aaggacatgc agaagtcgct ctggctgcgc gacttcgatg aactgatgga ggcgtttcgc
     8041 gaagtgttcc caattcgcga caatgcctcg atccaggaca tctttgccgc cttcgaccgc
     8101 tatcaactgc aactcagcgc cggtcgtgtc gacaccagtt ttgaacagag cgctgtccag
     8161 tcgtcggcaa aatcttctga aacccagcca tgaaggctgc ggagtcaatg ttgcgcggca
     8221 acagcgcggt caggggcagc tcagtgcggt gtcgttcggc ccgtggaccc ggacgcgtcc
     8281 accactgttg tagatcagtc cgcgcgcctg caggacatcg gcgcggcgcg ggcagaccgt
     8341 gaaggtgcca ccgattccgg ccatggtgcc gctggcctga tagctgaggc ggttggaggg
     8401 attggccgac agccgcaccg tcgcctccgg catcgcttca tgctgacgaa tcagctcttc
     8461 gccggcatcg agcagttgat tctcgttcgg atcactgaac agtgtccagc cgctctccca
     8521 gcccaccacc ggcggaccgg cactgcagtt ggcgcaacgc agcgtcaccg aattgccgcg
     8581 gcgcaccgcc tcgctgcggg ccagcgccag gtcgcgctgc aaatcctgct gaatcttgcc
     8641 cacctcgctg cgctcgacgc tgccccgcca gctcggcacg cccatcacca gcagcgtcag
     8701 cagcagcccc accaccagca gcagttcgat cagcgtgaag ccgccggcag tcgcgatacg
     8761 ctgccgggga gtcatggcgg agtcccctgc gcttcctgcc acttcaactc gcgcggcagt
     8821 gaaaagacca cctcctcgct gcgcccggca cgctcttcga cactgtcggc ccccagcgcc
     8881 agcagccggt cgatcagttg tcgcaccagc acgtcgggcg ccgaggcgcc ggcggtgacc
     8941 ccgacctttc ggcacccccg caaccagtcc ggttgcagcg cgccggcgtt ctcgatcagg
     9001 taggcggcca cccccatgcg ttgcgccaac tcctgcaaac ggttggagtt ggagctgttg
     9061 ggcgaaccga tcaccagcag caggtcgacc tcctgcgcca gtgcccgcac cgcatcctgc
     9121 cggttctggg tggcgtagca gatgtcctcc ttgcgcggtc cgccaatcag cggataacgc
     9181 gcccgcagcg cagcgacgat gcgggcggtg tcatcgagcg acagcgtggt ctgggtcgca
     9241 taggcaagcc gtgccggatc gcgcacctgc agccgttcga catccgccaa agattcgacc
     9301 agatgaatgg cgccgcccaa ggtcagatca tactgcccca tggtgccctc cacctcgggg
     9361 tggccggcat gaccgatcag cacacagtcg atgccgcgct ggctcagacg ggccacctcc
     9421 atgtgcacct tggtgaccag tggacaggtg gcatcgaaca gccgcagccg gcgcgcttcg
     9481 gccgcctgtc gcaccgcccg cgacacgcca tgggcgctga agatggcggt ggcgccttca
     9541 ggaatctcct cgacctcttc gacgaagatt gccccacgtt cgcgcagagt atcgacgaca
     9601 tggcggttgt gcaccacctc atggcggaca tagatcggcg tgccgaacag atcgagcgcc
     9661 cggttgacga tctcgatcgc ccggtcgaca ccggcacaga agccacgcgg gttggccagc
     9721 agcactttca ctgccgcccc tcctctgcct gaccattgtc aaccgacagg atctccacct
     9781 cgaacagcag tgtcatgccg gccagcgggt ggttgaaatc gatgcagacc cgatcggcct
     9841 cgaccgccag caccactccg ggcagttcac cgccgccagg gtcggcgaac gagatcacca
     9901 caccctgttc cagcgtcaga tcggcgctga aacggtcacg gttgaaccat tgccgattgg
     9961 ctggattgac tgcgccaaag ccctgctcgg gcgcaacctg aaagctgcgc cgatcaccgg
    10021 ccaccagtcc cagcagacag gtttcgaaat aaccagggag cgcgccgtct ccctgcacca
    10081 gctcggcacc gggaccacca aagttggagt cgatcagctc gccttctgcc gtcgccagcg
    10141 aaaagcagag cctgacccgg ctgccggcgc cgatggcctc actgcccatg gttggccgct
    10201 cgcgacgaag gggtggcgca gaacagagtt tcaagaatca gcatggtggc ccctgcggtg
    10261 atggcactgt cggccacatt gaacaccgga aatgcgtgcg cttcataatg gaaaagcaaa
    10321 aaatcgatga cgtagcccag cgtcagccgg tcccacaggt tccccagcgc gccgcccaga
    10381 atcagcagca gcgcgatgcg ctgccagtgg gccgaacgtg gcagacgcag cagccagagc
    10441 agaatcgcgc cactgatcac accggcaatg gcgatgaaga acccctgctg ccagccgccg
    10501 gcgtcatgca gaaagctgaa ggcggcccct tcattgcggg catgtatcag tgagaagaaa
    10561 gacaggatcg gccgcggttg atagagcgcg aaattggcga cgatccacgc tttgctccac
    10621 tgatcgatgg caacgaccag cagcgccagc ggcagccagc agagcatccg cagcaggtca
    10681 gccggctgga tgcgcgcctc aggcatggtg ccgtgcctca cccggaccct cgacattgtc
    10741 gacgcagcgg ccgcacaact cgggatgggc cgcgatggtt cccacctcgg gccgatggtg
    10801 ccagcagcgc acgcacttgg tgtgcggcga gcgcgccacc tcgatccaga gtccgggcag
    10861 ctcgctctcg accgccggca ccggcgcggc atcgctcgac agcggcagca gttcgacggc
    10921 cgaggtgatc agcacgaaac gcagttcgtc gcccaggcgg gccagatgca cgcgcagcgg
    10981 ctcggccaga tgcagccgca gctcggcctc cagactgccg ccgatgcgac cttcgctgcg
    11041 ggcacgctcc agttcgcggt taaccgcctc cttgacccgc atgatgcgct gccagtcatc
    11101 gaagctgaat ggatcctgca gccgctccag ctccggcagc cggtaccact gctccagaaa
    11161 caccgagggt gaccggctgc ccggcagatg ttgccagatc tcctcggcgg tgaagctgag
    11221 gatcggcgcc agccagcgca ccatcgcttc gatcacatgg tagtgggcgc tctgggtcga
    11281 gcgttgcggc agcgagtcgg ccggcgtggt gtatttgcgg tccttgatga tgtcgaggta
    11341 gaagccaccc aggtcggtga cgcagaagtt gtgcaatttc tggtagatca gatggaactg
    11401 aaagctgtcg taggcagcga tcagctcctc ctgcaatgcc agcgcgcgcc gcaccaccca
    11461 gcggtcgatc gccaccatct cggccaccgg cacccgatgc tgcaccggat cgaagccatc
    11521 gaggttggcc agcaggtagc gggcggtgtt gcgaatgcgg cggtaggagt cggcggtgcg
    11581 gttgaggatc tcatccgaga ccgtcatctc gccccggtag tcggtggcgg cgacccacag
    11641 ccgcagcaca tcggcaccca gcgtctggat cacctgctgc ggcgccacca cattgcccag
    11701 cgacttggac atcttgcggc cactggcatc gacggtgaag ccgtgggtca gcagcccgcg
    11761 atagggcggc gcaccagtcg cggccactgc cgccagcagc gaggactgga accagccacg
    11821 gtgctggtcg gagccttcga gatagaggtc ggccggcaac cccagctcgg cacgccgttc
    11881 cagcaccgcc gcccaggaga ccccggaatc gaaccagaca tcgagggtgt cgctgatctt
    11941 gccgtagtcg tcagcctcgt cgcccaacag ggtgcgcggt tcgagctcga accagccatc
    12001 gatgccttca cgctcgacga tcagcgccac ctgctcgatc agctcggcgg tgcgtggatg
    12061 gagctcaccg gtggtgcgat ggatgaagag cggaatcggc accccccagg cgcgctggcg
    12121 ggagacacac cagtcgggcc ggccgttcaa catcgcgctc atccgcgcgc ggccccaggc
    12181 cggttcgaac atcacccctt cgagcgcagc ctcggcggca tcgagcagcc cctgctgctg
    12241 catggcgatg aaccactgcg gggtggcgcg aaagatcacc ggcgtcttgt gccgccagca
    12301 gtgcggatag ctgtggttca gcggccggtt ggccagcagc cggccgcgct cgctcagcag
    12361 cgtgatcacc tgttcgttgg cggcaaagac attgaggccg gccagaaact cggtgtccgc
    12421 cttgaaacaa ccgcggtcat cgaccggatt cagcacttcg agcccatact tcaggccgac
    12481 ctgataatcc tccagaccgt ggcccggggc ggtgtgcacc gcaccggtcc cggcttcggc
    12541 cgtgacatgt tcacccagga tcaccggcac ctggcgcgcc aggaacggat gcagcagcca
    12601 ctgcccttcg aggtcgctgc ccggcgtgcg accgatgatg cggtagtgct cgatgccggc
    12661 gcgggccatc accgcggcca ccagcggctc ggccagcacc cagcgctccg gaccctgcgg
    12721 ccccgatgca gtcaccagca gatagtcgag ccgtggattg agtgcgaccg cctggttggc
    12781 cggcagcgtc cagggtgtcg tggtccagat caccaccgag accgggccgc gcccgggctc
    12841 ctcggtcagt tcccggaacg cacccagaaa gcgctcctct tcggccacga cgaagcggac
    12901 atcgatcgcc ggcgagcgct gctcctgata ctccacctcg gcctcggcca gcgccgaacc
    12961 gcagtcgatg caccagtgca ccggcttgaa gccgcgcagc aggtggccac ggtcgatgat
    13021 gcgccccagc gtgcggatga tgtcagcctc gaaccggggt gccatcgtca ggtagggatc
    13081 gtcccagtca cccagcacgc cgaggcggat gaaatcctgt cgctgccggt cgatctgctt
    13141 gctggcatag tcacggcagg cagcccgaaa tgcccgagcc gacaccttgt gtccggcctt
    13201 gccgatcttc ttttcgacat tgagctcgat cggcaggccg tggcagtccc atcccggcac
    13261 atagggcgca tcgaacccgc tcaacccctt ggccttgacg atgatgtcct tgagcacctt
    13321 gttgaccgcg tgaccaaggt gaatctcgcc attggcatag ggtgggccat cgtgcagcag
    13381 gtagcgcgga cggccggcgc tggcctggcg gatctgccga tagagctgct gcgtctgcca
    13441 ctgcttgagc agttgcggtt cacgctgcgc caggttcgcc ttcatcggaa agctggtctt
    13501 cggcaggttg agggtctctt tgtagctact catgaaccga atcgatactc ctgaaacaac
    13561 agtggacggc cgtctgcgat ctcatgggcg cagcatggcg gtgtcgaagt ggtggcgggc
    13621 cgcggccaca tcggcggcaa tcgccgccgt cagcgcggca aggtcggcaa acttgcgctc
    13681 cgcccgcaga tgcttgcgaa aggtcaccgt cagtctgcgg ccatagagcc ggccgtcgta
    13741 atcgagtaga tggacctcga gcagcggttg caaaccaccc accgtcggcc gcatgccgac
    13801 gttggcgacc ccgggatgct gcacaccctc caactccacc tcgacggcga agacgccgtt
    13861 gagggcggga cggcgccccc gcaaggagag gttggcggtg ggaaaaccga gctgtcgtgc
    13921 cagcttctgc ccttcgatga cccgaccaca gaacgcatag ggtcgcccca gcaggagctc
    13981 ggcctcatcc agatcggcct cgcccagccg ttcgcggatg cggctgctgc tgatgcgctg
    14041 ctgcagcgcc accacggtgt cggtgttggc aacggtgaaa ccaaagcgtt cacccgccgt
    14101 gcagagtgcg tggtagtcgc cctggcgctg atggccaaag cggaaatcat cgccgacgat
    14161 cagatggcgc acgccaacgc cctcgaccag cagccggtcg atgaactcat ccaccgccat
    14221 gccgctgagg tgacggttga accgcaggca gagcacccga tcgatcccct gctgtcgcag
    14281 cagtgccatc ttgtgggcaa agggggtcag tcgcggcggc gcctgattcc cggcaaagaa
    14341 ctcttgtggc tgcggctcaa aggtgatcac catcgacggc aagtccagct cggccgcgcg
    14401 cagtcgcaga gcgtgcagga tctgctgatg gccgcagtgc acaccatcga aattgccgat
    14461 ggtggccacg cagccgcgat gccgtggcct caggttgtgc attccgcgaa tcagctccat
    14521 gacgcctctc gaaccctggc cgctggcctg tcgtgatcga ccatcaaaag tggccgatta
    14581 tagcgcaaca tcacggcccg ccccgtgcgg ccaccgcacg cctcaggagt gccgacagtg
    14641 tcgacctgat cagcggttcg cccagcagca gcaatgccag cagatagccg gtgaatccac
    14701 cgatgcacag cagcgtcacc cgctcgaccc gctgccagac gccccaggcg gcccacaccg
    14761 aagcttcggg cgacagccac cacagcagcg cacccatcaa cagattggcc agcgcgatgc
    14821 gcaacacgaa gggcagccag cccgtcgcca gccgatagat ccgtccggtc agcaggcccc
    14881 gcagcagcag tccggcgttg agccagccgc tcagggcggt cgcgagcgcg agcccggcat
    14941 gctgcaacgg ccagatcagc agcaggttga gcagcatgtt ggccaccatc gcgatgatgc
    15001 cgatgcgtac cggtgtgcgg gtgtcctgcc gggcaaagta gccggtcgcc accaccttga
    15061 tcgacatgaa ggcaatgaga cccaacgcat agaacttcag gctgaacgcg ctcttttcga
    15121 catcgcgcaa tcccatttcg ccatactgga aaaaggtgaa gatcagcggc tcggccagca
    15181 gcaccagcgc cacggtcgcc ggcagaccga tcaaaagtac gccgcgcaat ccccagtcga
    15241 gcgtggcgga gaaggcctcg atctcctgcc gtgccgcgcg gcgtgacagc accggcagga
    15301 tcacggtcgc gatggcgatg ccgatcaccc ccagcggcag ctccaccagg cggtcggagt
    15361 agtagagcca ggagacgctg cccgtctcca gaaacgaggc gaggatggtg tcgagcagca
    15421 gattgatctg actcaccgag accccgaaga tcgcaggcgt catcaaccgg ccgatccgcc
    15481 gcacccccgc atggcgccag tcgagccgcg gccgcggcag cagccccagc cggagcagaa
    15541 acggaaactg gaacagcagc tgcaccaggc cagccgccag caccccccag gccaatgcgt
    15601 ggaccggcac atcgagccgc ggcgccagcc agagtgccgc cgcgatcatg gtcaggttca
    15661 gaaagatcgg cgtgaccgcc ggcacggcaa aacggccgaa actgttcagc acactgccac
    15721 agaacgccgt caatgaaatg aagaacagat agggaaaggt gatgcgcagc atctcggtgg
    15781 tgagcgccat cttggcgcca tcctgctgaa aacccggtgc aaaaagcagg gtcagcagcg
    15841 gcgcacccac caccgccagc agcgtcacca gcatcagcgc cagcgtcagc atgccggcgg
    15901 tcttgtcgat cagttctcgc gtctgctcca gcgagcgctg ggtgcggtac tcgctcagaa
    15961 ccggcacgaa ggcctgggca aaggcgccct cggcaaagag ccgacgcaga aaattgggga
    16021 tcttgaaggc cacgaaaaag gcatcggccc cggcgctgga gcccaccatc cgcgccacga
    16081 cgatgtcgcg caccatgccc agaatgcgcg acagcagggt catcagcgcc accaccgcgc
    16141 tcgatttcag cagggctgtg ctgcgccgct cggcctgatc catctttgtt ccaattgaga
    16201 agcaagttgc ctagaatggt gcgcgacttc gatcgtcctc aagcgcagag cacaaccgcc
    16261 gttgtccgcc gttcctgcat ggaacgacac gcaatgcaac actggcccac gaccaccacg
    16321 cgctggagca tggtccggcc actgccgact gccagcagcg acctcgcaac gaggcgaaac
    16381 aagccaataa aaagattcaa tcgtaacaca ggagagactc gaccatgact atcagctttg
    16441 aaggcaaagt cgccatcgtc accggcgccg gcaacggcct gggccggcaa catgcactgg
    16501 aactggcgcg ccgtggcgcc aaggtggtgg taaacgatct gggcggcgcc gtggatggca
    16561 gcggtggctc cagcaaggcg gccgaggcgg tcgttgccga gatccgcgcc tttggcggca
    16621 ccgccatcgc caatggcggt tcggtctccg acaaggccgg cgccaagagc atggtcgatg
    16681 atgccgtcaa acacttcggc accgtgcaca tcctgatcaa caatgccggc atcctgcgcg
    16741 acaaaagctt ctcgaacatg accttcgacg atttcgaggc cgtcatcaac gttcacctgc
    16801 tgggtgccgt ctatgtcacc aagtactgct gggagatcat gaaggagcag aagtatggcc
    16861 gcatcctgat gaccacctcc ggctccggac tgttcggcaa cttcggccag accaactaca
    16921 gcgccgccaa actgggcatg gtcggcttca tgaacaccct gcgcctcgaa ggagccaagc
    16981 acaacgtcta caccaactcg ctggcaccgg ccgcactcac ccgcatgacc gaaaacctgc
    17041 tgcccggcga agtggcgcaa gggctggccc ccgaactggt caccccgggc gcactcttca
    17101 tggtctctga ccaagccccc aacggcgtca tcattcaggc gcagggcggc tcatttggcg
    17161 ttgcggcgat tctgcagaac gacaaggtga gcctcggcga ccatccgacg gtcgaagacg
    17221 tggctgccaa ctgggagcgg atcagcgatc tctccaaggt cggcacccgc accgaaaaac
    17281 tggtcagcca gccaccgaag aagtgatccg ccgccgtcgg gcgcgtttgc gcaccccgat
    17341 ccggcaacca gacgcaaaac ccggcccgct tacgcggccg ggtttttttc gtgcctatcg
    17401 acaaagcccc gcaccgcgct tagagtcgat atcccgtgca gtctgttacg catggtgtcc
    17461 tgaccacaac ccaggaggca ccgatatgga ggtcatccat caagccgaac acgaacgttt
    17521 cgtcgttcag gtgggcgagt tcgaagccct gctcgaatac catctgctgc cccgacaggg
    17581 tgtggtcaat ttccaccaca ccttcgtgcc accccagata cgcggtcgtg gcattgccga
    17641 aaaactggtg cgcaccggct tggcctgggc gcggacgcag cagctcaaaa tcgaggcgag
    17701 ctgctcctat gtgcggaaat tcctgcgctg accgtgcttg gcctcaaccg ccggtccaat
    17761 tgacagcgcc aggctcgatc tggataatgc gcgcctgttt tacctcaatg ctcagaggag
    17821 ccattcgtgc ctaacattgc ttccgccaag aaacgtaccc gtcaagcgga aaaaaaccgt
    17881 caaaacaacg tcagccttcg ctcaaaagtc cgcacctaca tcaagacagc ggcaaaccag
    17941 atcgaagcgg gtgatgtcga gaaggcacaa caggcatttc agcgcgctgt tccgatcatc
    18001 gacagcatgg tcaacaaagg catcatccac aagaacaagg ccgcacgcca taaaagccgc
    18061 ctgagcgcca agatcaaggc cctcgcactg gccgcaaagt gacggcgtaa gcaccgccga
    18121 gccatgcaat aaaaaaccgg ccaaggccgg ttttttattg cccgcagaaa acgcctctct
    18181 acagcaacac cagattgtcc cgatgaatca gttcgggctc gcccatgtag ccgatgagtt
    18241 cagcaatgcg tccactgggc tgcccgatca gcttgaccgc ctcttcatgg ccatagttga
    18301 ccagaccgcg cgcgactgac ctgccggcct catcactgca aaccaccatc tcgccgcgag
    18361 agaaagtccc ggagatcgcc ttcacgccga ccggcagcag gctcttgccg cactctcgca
    18421 gcatgcgcac tgccccggca tcgatggtga tgtcaccgcg ggtctgcaag tgggccgcaa
    18481 gccactgctt gcgcgcagcc agcggatcct gctccggcag caacagagtg cccaacggct
    18541 cgccctcgtg aagtcgtaga atgacatgtg gcagcctccc accggcgatc agggtcatgg
    18601 caccggaacg ggcggccagc cgcgccgcct gcaccttggt caccatgcca cctcgcccca
    18661 gcgccgtgcc gctgccgccg gcaatctcca gcagatgggg gtcggtcgcc agcgcgccat
    18721 gaatcacctc ggcatcgggt tggcgtcggg gatcggcctg atagagcgca tcctgatcgg
    18781 tcaggatcag cagcatctca gcaccgacca gattggtcac cagcgcaccg agggtgtcat
    18841 tgtcgccaaa gcgaatctca tcggtgacca cggtgtcgtt ctcattcacc accggaatga
    18901 cgccaaggcg cagcagctct ttcagggtgg cccgggcatt gaggtagcgc ttgcggtcgg
    18961 agaggtcatc gtgggtgagc agaatctgcg ccgtatgcag cccaaaccgg tgaaagtagg
    19021 tctcgtaagc ctgaaccagt cccatctggc ccaccgcggc agccgcctgc agctcatgga
    19081 tcagttgcgg gcgctgcttg agacccagcc gcaacatccc ctcggccacc gcgccggaag
    19141 agaccagcac cacttcgacg cctcgacgac gcagttcgac aacctgcgcc acccaggccg
    19201 acatcgcttc ggtgtcgagc ccccggccat tggccgtcag cagcgcgctg ccaatcttga
    19261 tgacccagcg gcgcgtcgaa cgcagtgtcg cccgcgcagc aacaaattcg ccgacagaca
    19321 ccgcaccgcg cttcgagtcg cgactatctg acatagaaaa cctcagcctc ctcgtcatcc
    19381 tcatcttcat cttcatcgtc aggcagcaac tgctggctgg ctcgccagtc acgaacccgc
    19441 ttgcgtgcct cctcttccat gcgtcggcgg gtttcgagtt cgcgttgccg ggcttcggag
    19501 tcttccgcct cctcctgatt cacctgatca aggtactcct gcaactgctg gcacagcaat
    19561 tcggtgcctt gatgggccag cgcggagatc agatggagcg gcccctccca tggcagctcg
    19621 gcgcgcatgc gctgcaccag ttgctcaacc tcttcgatcg gcagcagatc gatcttgttg
    19681 agcaccagcc agcgtggccg tgccgccagg gtcgggctga attgctccag ctcatcgatg
    19741 atcacccgcg cggcatcaac cgggtcggag tgatccagtg gcgccacatc gaccagatgc
    19801 aacagcagac gggtgcgggt caggtgtcgc agaaagcgga atcccagacc agcgccttgc
    19861 gaagcccctt cgatcaagcc aggcacgtca gccaccacga aactgcgatg cggctcgatg
    19921 ctgaccaccc cgaggttggg aatcagagtg gtgaagggat aatcggccac cttgggacgc
    19981 gccgccgaga ccgcgcgaat gaaggtcgat tttccagcat tgggcaggcc gagcagaccg
    20041 acatcggcca tcagtttcag ttcgagacgc agctgccgtc gctcaccggc tccgccactg
    20101 gtggtcttgc gcggtgcgcg gttgacactc gatttgaaac gggtgttgcc aaggccacgg
    20161 tgcccacccg cggccaccag cagccgatcg ccagcctgca ccagatcacc gatcggctgt
    20221 tcgctgtcga catcgatcac ggtggttccc accggtacct tcagaatcag atcggcacca
    20281 ttgtgcccgg tgcagttggc gccacgccca ttctcgccat gcccagcccg atagcgcggc
    20341 tgatagcgaa aatcgaccag cgtattgcag ttggcatcgg cctcgagata gatgctgcca
    20401 cccgcgccgc catcgccacc gtccggcccc ccacgctcga tgtatttctc gcggcgaaaa
    20461 ctgagacagc cattgccgcc gtttcccgcc tcgacccaga tcgtggcttc atcgacgaat
    20521 ttcacggtgg caactcctgc acagggatgc gatggtcagc attacggtat gcaaagcccg
    20581 aactataccg caagcatgcg gtgagcaagc gtcctgacgg aggtgcagaa aaaaattcag
    20641 cggcccacgg atgggtacac cagatggccg acgacagggg gcgggcattt catccatcaa
    20701 caaaaaggcc ccggatcact gggaccggag ccttttgact gacaggtgtc agactggaac
    20761 gatgttgacg taacggcgct gcttgggtcc tttcacttca aactggactc gaccctcggc
    20821 cttggcgaac agggtgtggt cggtgccaca gccgacattc gcgcctgggt gaaatgccgt
    20881 accgcgctga cggacgatga tgctgcccgc cttggccagt tgcccgccat agagcttgac
    20941 gcccaggcgt ttggattccg aatcgcgtcc attgcgggta ctgccgcccg ccttcttgtg
    21001 tgccatgact cgctacctct gaatgatggg ttgtaacggg ttcgatcagg ccttgacgac
    21061 cgcaccgatc gccatgatct tgacctcggt gtaccactgc cgatgtccag cctgcttcat
    21121 gtggtgcttg cggcgacgga acttgatgat cttgatcttt ttcagccgcc catggccgat
    21181 gacttcggca gtgatgttgc tgtccgtcag gtaaggcgcg ccaaccttga catcactgcc
    21241 ctcacccacc agcaacaccc gatcgaattc gaccgactca ccgggcgcct gctcgatctt
    21301 ctcgagcctg atccggtctc ccagcttggc ccgatgttgc ttgccgccac tttcgaatac
    21361 cacgtacatg ttcttcgcca ctccccgctt attctttcga ccacaacaga ggacgagcag
    21421 aaatttgccg agccttgctg tcaagggcag ctattttacg cataaaggac caacgactca
    21481 acatgatctc ttcagatgaa tgatggcaaa catggctgaa ctcgcccgtt gccggcatcg
    21541 aaggcctgag ttgcgcacct gaacgcttga gtcaaattgc ccacagaatc agcaagcact
    21601 tacgcagcgc tgatttgtgc attgatcaca tgcagctttg ccactggatc ggctgccgcg
    21661 gtcaccgatc tgcccaccac cagatagtga ctgccggcac gaatggctgc cgcagggtcg
    21721 agcacccgct tctggtcatg ctgctcatct cccggcagtc tgattcctgg cgttacccgc
    21781 aaaaacgcag catcgacttc gggtttgagc attgccagat cctgcgccga gcagaccacg
    21841 ccctgcagac cggaatcctc ggccagtttg gccagcgtca ctgcccagtc ggccggcgtg
    21901 tgcgccacgc cgatttcgtt caggtcatac cgatcaagac tggtgagaac ggttacggca
    21961 atcagcaacg gcgccctggg atatctggcc aaggcattgg ccgcgctttc catcatgtgg
    22021 cggccgccac tggcatggac gttgaccatc cagaccccca gttccgctgc agcctggacc
    22081 gcccgtgcga cggtctgcgg gatgtcatga aattttaggt caagaaagac ctcatagccc
    22141 tgctcgatca gcttgcagac aaattcaggg ccactgcggg taaacagctc ttttcccact
    22201 ttcaaccggc agagctgcgg atcgagcatc tcgaccagcc gcagggcggc ctgcgggtca
    22261 tggtagtcga gggccagaat ggttctcgga cctgacggta agctcatcgg tactccaggt
    22321 tttgaccaaa gatgggtttg atactgttcc attcccgaca gcttgggcat tgccaatgca
    22381 cctttgtgcc tttgaaaccg caatggctgc acagatattt cgatttttcc tccatgaact
    22441 gatcggacaa ctgcttgaga ctcctgaaca gcatggccaa atccgtcact tccggctgaa
    22501 agtggcgctc cagcaggtcg agcagaggcc tgaaacttgg gaaacggtgc aactgcagca
    22561 gcaggaaatc ataggttcga cgtggcccat gcacctgcaa ctgaaactcg gtcgtcacca
    22621 tgatcatggc caccgagggg tattcatcca gccagttcag catccactgc tcgaccatgg
    22681 cgaccggcgc caatctctgg atgcgatcga ggaacagcac gacggtatca gccagccact
    22741 cgggcgcctg ctgccgcacc cgccgcagat gtcgcaatgc agcctcctgg ttgctcaatt
    22801 cgagttcaag tgcggtcagc aaccagcttg cacgcaccga cttccgatcg agcttgagcg
    22861 ccgctgccag ggctgtgcga acagccgaat ggtcggcggt cgccaagccc gcttcggcca
    22921 gctcacagtg ataatgggcc aatgcgcagt gcagggcgac aagccgctgc tgatttttgg
    22981 catcaaggag cagcgcgggc gaacgcagca gagtgcgcat ccgtgcaccg accgcgatcg
    23041 cctcatgcca ctcacggctg ccttgatgaa gctccagcag cgcctccatc gcggcaatgc
    23101 gcaggaccgg atagcgctca accagatcat tcaacagcaa ctcggcccgg tcatgaagcc
    23161 cggcatggat gtaatctttc gccaactcga aaatcagctc gccatgcaga ggcgaatcga
    23221 ccgcaaaccg gttcagcagg gtctgatgac actcaatggc atactcgacc tccccacgcc
    23281 gacgcagcag cgccgcgatg acgaggtaga actccacacc gctttggtcc tcgtcgagaa
    23341 tcttggacaa cgcgttgaca aacgaactct tttcgacagt gatcaaacgg tccagatcgg
    23401 acaggtagcg cgcctggctc gctgctggct cggtgcgcaa gcgcaatgtc atcgaacggc
    23461 gtccaaacag ccagcccagc agcaccgctg tcagcagcag gcaaaagagc aggagcgtat
    23521 cactcatcga tgcgatgggc tcgcatcaac ggccggggtt gcagcacgct ctttttctct
    23581 ggcgttctga cgttcgacag acgacagccg caaccgccag cgtgccagcg caaccgaagt
    23641 gaccagcgac gcagcacagg ctccggcaaa gaaggcaagc accaaccagg cgcccaaaga
    23701 gagttgcgga agctgcaggc tccagagctg tggctgcact ggcgcgctgt tctcggtcat
    23761 gaaccaagcc gtcatcgaca tcagcagcag gacggcgatc cattggatgg agtgcttcaa
    23821 tcggcgcatg gtccgcttaa aaatatctgg agatacaatc tgctgaatgc gttttctcca
    23881 caagccaccc gatcaaagag cagcgatcga ttcgctttgc gaatcagcgc agggcaaact
    23941 tttactctat caggtcattg acccggtccc gcaactcctt gcctggcttg aagtgtggaa
    24001 cgtgcttgcc ctgcagcggc accgactcac ccgtcttggg attgcgcgca actctcggct
    24061 gtcgaaagtg gaggcaaaaa ctgccaaagc cacgcacttc gatccgttta ccggtggcca
    24121 gcacctgcga catctgctca agaatgcagc gaaccgccaa ctcgacatcc ttgagtggaa
    24181 gctgcggctg cttcgcagcg atacgctcga taagctcgga tcgggtcata gggattccgc
    24241 tctggcatcg ttggctattt tcatctattg atcagcgaca tggactttac catgaagccc
    24301 atgtcgcgat catggccatc cttggccaac ctgctcagcc ctggttttcc atctgcgcct
    24361 tgatcagatc accgatggtg gtcggcgata ccgcttcggc ctcctgcttg cgcagctctt
    24421 tcagtgcctg tttctgatcc acggcatcct tggcacggat cgacagcgca atcgcccgcg
    24481 tcttgcggtc gaggttgacg attttggctt cgatcacatc acccggcttc agcagttgcc
    24541 gcaggtcgtc gatgcgatcg ctgctggctt cggaagcctt gagcgccgcc tcgacaccct
    24601 cggccaactg aaccaccgca cccttggctt ccacctcctg aacggtgccg gtcacgacgc
    24661 tgcctttgtc atgtgcatcg gcatagtcgt tgaacgggtc gctcagcagc tgcttgacgc
    24721 ccagcgaaat gcgctcacgc tcgggatcga tcgacagaat gacggtttcg atgtcatcgc
    24781 ccttcttgta atgacgaacc gcctcttcac ctgcctcatc ccaggagatg tcggagagat
    24841 gcaccagacc atcgatgttg ccttccaggc cgatgaagat gccgaagtcg gtgatcgact
    24901 tgatcttgcc ggagatgcgg tcaccacggt tgtagctttc ggaaaacttc tcccacggat
    24961 tctgcttgca ctgcttgatg cccagcgaga tgcggcggcg ctcttcatcg atatcgagca
    25021 cctgaacctc gacctcatcg ccgatctgaa ccactttcga cggatggatg ttcttgttgg
    25081 tccaatccat ttcggagatg tgaaccagac cttcgactcc ctcttcgatc tcgacaaagc
    25141 agccatagtc ggccaggttg gtgaccttgc ccatcacgcg gctgccaacc ggatagcgct
    25201 ggatgatcga gagccacgga tcttcaccca gttgcttcag accgagggag acgcggttgc
    25261 gctcgcggtc gaacttcaga atcttgacgg tgatctcatc gcccacgttg acgatttcac
    25321 tcggatgctt gatccgtttc caggccatgt cggtgatgtg cagcaggcca tcgacgccac
    25381 ccagatcgac gaaggcgcca tagtcggtca gattcttgac gatgcccggc acctccatgc
    25441 cctcttgcag cgacaccagc agcgcttcac gctcggcact ggtggcctgt tcgagcacgg
    25501 cacggcgtga aaccacgacg ttgttgcgct tctggtccag cttgatgatc ttgaattcga
    25561 gtgtcttgcc ttcgagatcg accgcatcac ggaccggacg aacatcgacc agcgaccccg
    25621 gcaaaaaggc acgaatcgtt tgaatgtcaa cggtgtagcc gcccttgacc ttgccgttga
    25681 tcagaccctt gaccaaggtc tcatcggtga aggcctgctc cagatcctgc cacacctcca
    25741 cgcgcttggc cttttcacgc gacaggcggg tttcgccata gccatcttca atggcttcaa
    25801 gtgcaacctt gacggtatcg ccgatctgaa tggtcagctc gccgttttcg ttcagaaact
    25861 gattcttggg aatgacacct tcggacttca ggccggcgtg gacggtgatc cagtcgctgt
    25921 cgatgtcaac cacagtgccc gagacaatgg aaccgggttg catgttgatc tctttcaagc
    25981 tctgctcgaa gagttcggaa aaactttctg ccatgggttt acctgttgat gaattcgcca
    26041 atgccacctg cgggtggtca ccggcaccgt taccgtgctc cagccagcac ggtctgttga
    26101 tcaaagggat cctcttctcg cagtgcgact ggggagagcg caagaagagc gatcggttta
    26161 cagcaggcgg cggtttttgg cctcttgcca gacgcgcgcc accacctcat cgataccgag
    26221 atcggtcgtg tcgatgacca atgcatcgtc tgccggtcgc aacggcgcca ccgaacggct
    26281 ggcatcgcgc tgatcacggg cttcgatctc ttcaatgagg cgcgtcaggc taacatccag
    26341 gccctgctct ttcaactgcg tccgccgtcg ctgcgcgcgc accgccgcgc tggcggtgag
    26401 gtagatcttc agcgccgcgg cgggaaagac caccgttccc atgtcccggc catcggcgat
    26461 cagacccggc gcacgctgga agccatgctg aaaatcgagc agtgcggcac gcacctgtgg
    26521 atgcaccgcc acctgcgagg cgcggttgcc gcactcctcg ctgcgcaacg ccgcatcgac
    26581 gacttggccg tcaagtcgga cctgtgtgca cccgcccagt ggatcgatgt cgaagcggac
    26641 atccatggtc gccgctgcct cagccaagcg cgcctccgcc gacagcggga tctcctggcg
    26701 ctgggcgatg agcgccacca gccgatagag ggcaccgctg tccagcagat gccagccgct
    26761 ctgacgggcc agatggagcg ccacggtgcc cttgcccacc ccgctcgggc catcgagggt
    26821 gatgaccggt ggcggctccg cttccactct acccatccgc gttttcgacc ctgatgccga
    26881 gtcccgcctg ctgggccgat gccgcgaaat tcgggaatga ggtggcgaca ttggcgcaat
    26941 cggtgatctg aagtggcgcg ctggcacgca gcgctgccat ggcaaaggcc atggcgatgc
    27001 ggtggtcgcc atgactgtcg atgcgcccgc caccgaacgc gccgccgcgc agcacgatgc
    27061 catccggccg cacgacactg tcgacgccca gtaccttcag accatcggcc atcgcctgga
    27121 tgcggtcact ctccttgacc cgcagttcgc tggcgccgct cagcacagtc tcgccagtgg
    27181 cctgcgccgc cgcgaccagg atggccggaa attcgtcgat cgccagcggc accagcgctt
    27241 cgggaatttc aatgccacgc agcggtgcat ggcggatgcg cagatcggcc accggctcat
    27301 cgccggcgct gtactggcgc tccagggaga tgtcggcacc catctgccgc aggatgtcga
    27361 tgacgccggt gcgggtcgga ttgatgccga cgttgcgcaa cagcaggttc gaaccgggcg
    27421 caatggtggc gccgacaatg aaaaaggccg ccgacgagat gtcgcccggc acctggatgt
    27481 cgatggcgcg cagcgtgcca ccgccttgca gcgagatccg ctgtccggca gcgactggat
    27541 gactctcgac ccgatagccc aggctgcgca acatccgctc ggtgtggtca cgggtcggcg
    27601 ctggttcgat caccgccgtt tcgccttggg catagagccc cgccagcagc agg
//
LOCUS       DAORCQ010000027        25154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1127_length_25154_cov_5.592494, whole genome shotgun sequence.
ACCESSION   DAORCQ010000027 DAORCQ010000000
VERSION     DAORCQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 25154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25154
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_25154_cov_5.592494"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1616
                     /locus_tag="PKI23_04515"
     CDS             219..1616
                     /locus_tag="PKI23_04515"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNN36239.1"
                     /translation="MIALRLKQFLDEQAVSYRLIEQPQGRSMEEVARLALIDPVLVAR
                     AELFFDGTTLLMVVSPLTYQIDMQRLDRLVGRPLTALSPRNSERFFPDCEPGCYPALG
                     ASYGLPVVMDAFLARFPKVYIATGHHTGLIELEGDQFRRLHGAVLEGDFTLKPARVAP
                     EPTESEAAPIDHERLKRKIERSGRLPAMPDLALRILQLVRDPEFSTAELGRLVAQDPA
                     ISAQVMKTASASAYAYQGKINSIDVAIVRVLGYRMVTHIAVGMAVSRSFRIPEEGPLG
                     LKRFWLRAIFCATACQMIAQRLPKTLGVDAGLAYLTGLLHQFGLLMLGHLFLPELRML
                     GKLAEVSPEEPLHQLERRILSEGLGADALRLGHARLGAWMLQLWNMPAELQAVVAHYE
                     ARDYAGEHALYHATLLLATDMFSAHLLGHEQLGPASTAPLARLGLSSQQINDIHSSML
                     DQITLIEELGAALAA"
     gene            complement(1619..2251)
                     /gene="rnhB"
                     /locus_tag="PKI23_04520"
     CDS             complement(1619..2251)
                     /gene="rnhB"
                     /locus_tag="PKI23_04520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNN36240.1"
                     /translation="MPQPPSRLETLLQGHAAARIAGADEAGAGPLCGDLFAAAVILDP
                     DQPLSGLDDSKRLSEQRREQLAEQIGRHAIAFAVARVTVEEIDRHNILHARMIGLARA
                     VALLDPAPTLALIDGNRLPGQLCCQGEAVVQGDRLIPAIMAASILAKVTRDREMRQLD
                     QYYPGYGLARHKGYPTAAHLAALQRLGPTPIHRRSYAPVRRLLETGAESP"
     gene            complement(2251..2655)
                     /locus_tag="PKI23_04525"
     CDS             complement(2251..2655)
                     /locus_tag="PKI23_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNN36241.1"
                     /translation="MQPDFVDANGRSGRFSDHANQWLLINYWASWCAPCREEMPQLNQ
                     LAKERSDQLALFAVNFDQLADDELRKQAQAIGIEFRVLRTDPIQQFGYQRPTVLPVTY
                     LISPDRRQTIQLLGPQNVASIEAALAKASATP"
     gene            2774..4372
                     /locus_tag="PKI23_04530"
     CDS             2774..4372
                     /locus_tag="PKI23_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36242.1"
                     /translation="MNHITQASPTAALQLTVPLLIWGSHDNTLDLTQLMRQLDDPAGR
                     QPERLMSDVFDLVRRSNRIPMEDQSRALLLDCAGRCQQFLLVQYRAARRQIGMAASSN
                     DGQSERLLRLTEELGFGYKRLLLSQIETFAPVEQQAVTLRGAITALSQQFLLCLDLRR
                     ARSKSLWGEFHRLFHHAERIKVENRQFREVDGAAGTNLHGLYLRTVLTFAADPYALPA
                     NEHWLLQSYLARWCSLVRLDPAMQPPSFSQSSRMAVDLRGLEIGPLLEQVQQHRFQLA
                     AGEQSVQALGFEAQVSTDQALRLLDHLLERWTQKRQRGAERHACQTTVTLTVGFMSIY
                     RCLRGSVTGQQETSTQLQFDAVMENESDGGAALRLPTDYRGTLPAVGELVLLAKRDGF
                     DPVRPMLGWVRWQQRSPRGEISFGMEYLHSTTQPVELDQFDGSTLSASQRPALLLVRA
                     PGESLPFSLIAQPHIYRSDRTVRLAFVQRIETQQAVAGRMIARSGSCDHFQLEGDLDV
                     DSTLPDEVIEIDYDPFPFNLGSLS"
     gene            complement(4381..5787)
                     /locus_tag="PKI23_04535"
     CDS             complement(4381..5787)
                     /locus_tag="PKI23_04535"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNN36243.1"
                     /translation="MLQDFFKAVRSHDITISAAESIEASRTAELIGFDDREILKLALS
                     VTLAKSVDEKARFNDCFDEFFTFNSFQQGEESASSASPAKAPANESQPPGQSAEEQVA
                     SHYEGKSELVKMVMERNRVALANEMKRAARSVGVTDIWLFTQKGIYTQKILKEMGMQE
                     INQEIAELANTTCPRGKAQAALLRQVRKLLLEEVRSFVEQQLSLYGSGTSKRLREEFL
                     MNTRLSNIERRDFHRMHEIVRRLAKKLSDTHAKKRKQTQRGVLDFRKTMRRNVAYDGV
                     MFETYWKTKNLERPRIVCICDVSGSVSAYARFLLLFLYSLNDVLMQIDSFAFSSHLVD
                     VNDIFKEHPVEQAIELALKEAGGGSTDYGQMLRDLKQNRIENSDNLRNIDHRSTVIIL
                     GDARNNYGEPRLDVMKLIYQRAKRVIWLNPEPQISWNTGDSVIGKYRPFCHLVKEANT
                     IKQLEGLVDNILKHSLAG"
     gene            complement(5800..6759)
                     /locus_tag="PKI23_04540"
     CDS             complement(5800..6759)
                     /locus_tag="PKI23_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNN36244.1"
                     /translation="MKELHDQRFTSVAEIEREFSNAGYICNREIATAIYIAFNLHKPI
                     LVEGPAGVGKTELAKTTASILDVPLIRLQCYEGLDESKALYEWKYGKQLLYTQLLKDK
                     LNELLGTTHSLAEAMGKLRQQDDLFFSEEFLEPRPLFKALHEERGCVLLVDEIDKSDH
                     EFEAFLLEVLSDFQVSIPEIGTIEARVKPIVFLTSNNNREMSDALKRRCLHLSIPYPE
                     PELERRILESRVPGLSERLREHLVSFVHQVRALDLKKLPSISETIDWARVLVLMHAES
                     LEPKLVQETLNTILKFQDDIEAVNEQLDALIADSEQPMAKTGG"
     gene            7056..8606
                     /locus_tag="PKI23_04545"
     CDS             7056..8606
                     /locus_tag="PKI23_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNN36245.1"
                     /translation="MMTLHNFWHWAEQTPARTAVIGPDGRQLTFAELDARVNQLSHGL
                     RALGYRRGDAVAVVIPNGIEWLEIFLATLQSGLYMVALNHHLVGAELAYIIDNCDARG
                     LIGHRRYGEALAAAVESLDFDPAQRFGIGGLPGFHDYESLLAGQPITPPAERSCGALM
                     LYTSGTTGKPKGVRRKLPEESPEEVAFKSSFLCLLFNGSVGSGSHLVQGPLYHSGPSG
                     FGMAALQAGQSLVIMDKWTAEAVLALIDRHRITATHLVPTHFERLLDLPEAVRARHDL
                     SCLTHVVHAAAPCPVGSKQRMIDWWGPVIHELYGATEGGGTSCSSQEWLQRPGTVGKA
                     WPVSQVKILDESGCELPAGTPGLIYMSTAIAQFEYHKDSEKTQRSRRGELFTTGDIGY
                     LDEEGYLFLCDRQSDMVISGGVNIYPAEIEGALLQHPKVRDVAVFGVPDPQWGEAIKA
                     VIEVSDGAEPGERLTDELKQFLEGRLARYKWPKSFDYVSTLPRTETGKLMKRTLREPY
                     WAGLERKV"
     gene            8645..9337
                     /locus_tag="PKI23_04550"
     CDS             8645..9337
                     /locus_tag="PKI23_04550"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HNN36246.1"
                     /translation="MENAEFIPLALAGVSFVAAHLLPSSLALRPRLVRILGENGYLAA
                     YTLFAFATFGWLCHAYVQAPHTVMLWNPGVLRFLPLLLMPVALWFIVCGVSGPNPSSV
                     RQEQKLAEAEVASGMLRITRHPVQWGIALFALGHLLANADLAALLFFGALLLLATLGM
                     YHIDQRKAATLGEPWQRFVAVTSLLPLGAVLAGRNRFVISELGGWRLLFAGALYLILL
                     LAHPWVIGVSPF"
     gene            9485..10246
                     /locus_tag="PKI23_04555"
     CDS             9485..10246
                     /locus_tag="PKI23_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNN36247.1"
                     /translation="MNASLSRFSAFFSRELAGALLILFTLFLQGCGAGYTAVKKRDLV
                     TQTKMSDSIFLDPVAPEKRVVFVEIRNTTDQQLQVEQAVRQRLSARGYRLTDNPDEAT
                     FLLQANILQVGRSDLRGSSDAISSGFGGAVAGAVLASATGSSNREAAGVGVLAGAASV
                     LSDAMVDDVLYTIVTDLQFRQRLAAGEQVSQQQSTTLKQGTSTELKESVTPAPTQWKS
                     YRTRIVSSANQANLKLETALPALEAGLVQALGGLF"
     gene            10528..10612
                     /locus_tag="PKI23_04560"
     tRNA            10528..10612
                     /locus_tag="PKI23_04560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10562..10564,aa:Leu,seq:tag)
     gene            10656..11987
                     /gene="tig"
                     /locus_tag="PKI23_04565"
     CDS             10656..11987
                     /gene="tig"
                     /locus_tag="PKI23_04565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNN36248.1"
                     /translation="MQVSVEAVSVLGRRLTIDVPVERIETEVSARLLKAARTMRLDGF
                     RPGKVPLKLVRERIGEGVRSEVLGDVINDSLNKAIVDQNLIPAGRPMVEAIDTEQPQD
                     LRFVATFEIYPTVDLADFSVLNVQRLSAEVTEADVDKMIETIRKSNADWTSVQRAAAN
                     GDQLTVDFEGFVDGEPLEGGSASGAKIVLGSQRMIPGFEDGLIGAEVGAERTLDLKFP
                     EPYHNAAVAGKDARFKVKVQEIEEQTLPEIGKALFERVEFDGDSVEAFRAEVRSNMER
                     EMNAALRRITKQRVMKALEEAHSFDLPGALVGSEIQQMRHQLLEQMGHRHGHDCDHDH
                     ESDFPSEIFAERAQQRVKRGLILAEIIKGVPIKSDPSRVRLFVEELASSYREPERVVE
                     WYYQNPEMISSIEALVLEDQAIDHVLANTQVSDETTTYEAVIREASEAANG"
     gene            12076..12693
                     /gene="clpP"
                     /locus_tag="PKI23_04570"
     CDS             12076..12693
                     /gene="clpP"
                     /locus_tag="PKI23_04570"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNN36249.1"
                     /translation="MIENVGLVPMVVEQSARGERAYDIYSRLLKERVIFLVGPVEDHM
                     ANLIVAQLLFLESENPDKDIHLYINSPGGSVTAGLSIYDTMQFIKPDVSTMCIGQAAS
                     MGALLLSGGAEGKRYCLPHSRMMIHQPLGGFQGQASDIEIHAREILLVRDRLNKILAR
                     HTKQDLETIARDTDRDNFMSAIEAVSYGLIDQVLEHRVDSLKSEK"
     gene            12770..14056
                     /gene="clpX"
                     /locus_tag="PKI23_04575"
     CDS             12770..14056
                     /gene="clpX"
                     /locus_tag="PKI23_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNN36250.1"
                     /translation="MVDERNGRGDEGGKLLYCSFCGKSQHEVRKLIAGPSVFICDECV
                     DLCNDIIREEIEESSNESSAEKLPTPRDIKKTLDEYVIGQSLAKKILSVAVYNHYKRL
                     GHNPKGKDDVELAKSNILLMGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGE
                     DVENIIQKLLQKCDYDVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKL
                     IEGTVASVPPQGGRKHPQQEFLQVDTSNILFICGGAFSGLDKVIRDRSEKSGIGFSAS
                     VKSKEDKKNVGETLKKVEPDDLVKYGLIPEFVGRLPIIATLEELDEAALIKILTEPRN
                     ALTKQYAKLFDMEHVELDFREDALRAVAKKAMERKTGARGLRSILESILLETMYNLPS
                     MKGVKKVVIDENVVVDESEPILLYENLEQQRAAPDQ"
     gene            14167..16500
                     /gene="lon"
                     /locus_tag="PKI23_04580"
                     /pseudo
     CDS             14167..16500
                     /gene="lon"
                     /locus_tag="PKI23_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            16647..16925
                     /locus_tag="PKI23_04585"
     CDS             16647..16925
                     /locus_tag="PKI23_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNN36251.1"
                     /translation="MNKSVLIDSVAVAAGVSKAAAFRTLDAMTELLAEKLRNGEQVTL
                     PEFGVFKVGRRMKSEEIDPEMHESCYFQSKNTPSFKVSRELKNILSSR"
     gene            16972..18858
                     /locus_tag="PKI23_04590"
     CDS             16972..18858
                     /locus_tag="PKI23_04590"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNN36252.1"
                     /translation="MLQNIRDGLSGIVSKAIVGFIVLTFVFFGIETLVQYKSESVIAT
                     VNGLDIPESTFRHAVELEKRNIIYRNQGVVPKIDEAVLASQVLDKLIDIKLLSGYAEG
                     LSVAAQKPQIDQLITAQAEFQVNGKFDPAVFKQVIESSGFTPQRYYENLKTDFVLNQL
                     SQAYLYSSFQSKNDWQDVAALVQQRRSFSTLDIPIERFFKDIDKDDPAIKSYYEAHSD
                     QFKTDEAVTVEYIELTIDQAMREVEISNDEVKARYEKEVETLSARKRLHAAHILVRAS
                     SENDLNAKTKIDKINEELRSGADFSELAKKYSEDKGSAGNGGDVGATDGSVFPPEFEH
                     ALQNLQVGEISSPIKTEAGYHLIRLLEKSDSKIPKLDELASSLHTELSRERARLILKE
                     KVENLSTVVFESPDLGEPAVRFGLSKQVAGPFGRDHGEGIAANLKVREQAFSEAVLSS
                     GQNSEVIELDEGHYVALRVLRHDPQRQLSLNEAAEKIRNIVSREAARTHAENVAKKSI
                     DELRLGKPIDEVAKNAGLKFRHYSKVLRSGDQDLDPQLLQSVFRAEIPVSGSVEIGSA
                     MLGDGDQVVFLLTEVISGAHDELTAEQQAQLVNFLAFGSGKRDFSSVQRQLKTQADIV
                     KNNF"
     gene            18943..19221
                     /locus_tag="PKI23_04595"
     CDS             18943..19221
                     /locus_tag="PKI23_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36253.1"
                     /translation="MNDLPMAGEWYCIIGQEEPFQVVAIDEESGLVEVQHLDGDIEEY
                     EIEEWEALDLEEIEPPEDSSAPYDNYSYDEDEEGEEIPDGFSLLGEEE"
     gene            complement(19431..20495)
                     /locus_tag="PKI23_04600"
     CDS             complement(19431..20495)
                     /locus_tag="PKI23_04600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNN36254.1"
                     /translation="MSAIERLCDYLPLARARLGEPLWRYLQEGSSDQAIDANARALAA
                     VRLWPRPLRDLSGGHTRLQLYGQQLDHPILLAPIAYQRLFHADGECASALAATAQGGQ
                     MLVSSLASQPFEQIAKAGHGEQGRPWFQLYWQGNRERTLRLLQRASAAGFSVVVLTID
                     APIKRSSLQLPNGVSAVNLDAVAPPEPVDPALSRVFDGWMKQAPTWNDVTWLRQQTKL
                     PLLLKGITHPDDADQALNLGCDGIIVSNHGGRVLDGAPASIDLLPAISARIAGRMPIL
                     FDSGIRSGHDLFIALALGATAVLIGRPFIWGLACNGALGVAHAVRLLRDELEMTMALT
                     GCARLSEIGPHCLADRAVVP"
     gene            complement(20497..21183)
                     /locus_tag="PKI23_04605"
     CDS             complement(20497..21183)
                     /locus_tag="PKI23_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNN36255.1"
                     /translation="MLITIDNLLTADELATSRDLLERAHWRSGLETAGIQAAVTKNNQ
                     QLSEGAEQLPALRQIVLNALTRNPLFFTAALPLKILPPLFNRYGDLTNSYGFHTDNAM
                     QRIAQQPGHYLRADLSATLFLSDPEQYDGGVLTIEDTFGNHGFKLEAGSLLLYPSSSI
                     HAVSAVTRGERLACFLFIQSMVRDPGQRRLLYEMDMALLTLRSEMGESDAVVRLTGLY
                     HNLLRRWAES"
     gene            complement(21199..23433)
                     /locus_tag="PKI23_04610"
     CDS             complement(21199..23433)
                     /locus_tag="PKI23_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNN36256.1"
                     /translation="MSYIEKKTELERNPATVPLLPLGAMMLAGMASLSAMAVDDKAAE
                     TAQQLSTVNVEAAVEQPDGYRATATRVGKLLQDPHEIPQAVTTITNSLMEEQQVGSLR
                     EALRNVSGLTFNAAEGGRSGDNMNLRGFYTFGDMYLDGIRDTAQYNRETFNYEQIDVL
                     RGAGAMLFGRGQAGGVINQVSKTPLRKAQYELTGSVGGENYREATADLNKPLSEDAAL
                     RVNLMQRNEGSWRSNPTTGNEPEIDREGVGLSLGLNLYSDNQFWINHYWLKTNDNPDY
                     GIPFDPITRKPTKAFSAETFWGTDATFDESNTQITTLVNEYRFSPDSQLRTQLRLADY
                     ERSYWARTPSLTSPPNPQALVLNGSVANGGPTRTADYETVTLQSDYSTAFSALGMAHL
                     FLTGVEYLNENSHRTTLRNLGGTTAGNPPYYRPYDPNPTAAPVNFDSDSYAFYAQDTV
                     EFIPQWKFTLGVRRDEMDADYSSQTSPSLAYGENSYRSALSFHPTTENHYYLSWSDSF
                     SPTADLYQLTVTPLPAERSEVVELGSKWLWFEGDLAFRAALYEATKEWERNTDLESTA
                     AILTKERRTRGLELEAAGRINERWEIFSGLALMEAEILEVAENVNATTGAITVANPAY
                     AGKRPRNTPPYTFNLWSSYKLTPQWKIGGGVEAKGERYGYNPSGTGAIPTLPGSSKFN
                     PNTAPAYARWDLMLSYEQPRWSARLNVKNLFNQLYYDALYDNGVFTVPGTRRIAILTG
                     ELKF"
     gene            complement(23512..23931)
                     /locus_tag="PKI23_04615"
     CDS             complement(23512..23931)
                     /locus_tag="PKI23_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010170950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNN36257.1"
                     /translation="MHSASPFEEDGDSALAEINMIPLIDIMLVLLVIFIVTAPLLTHS
                     VKVDLPKASSTPTLTPPENIQLAIDGAGQLFWNGEAIAAQELDLRLQAAAARSPQPEL
                     HLRAERTTAYEKVAEVMSTAAQHGLNRIAFVTEPRRQ"
     gene            complement(23941..24702)
                     /locus_tag="PKI23_04620"
     CDS             complement(23941..24702)
                     /locus_tag="PKI23_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNN36258.1"
                     /translation="MENSLGFVHFVANSDVVARFILLLMTLMSIGSWYLIVTKTVRLL
                     AMQRRGRTFLDHFWQAENLDTLTRLCDGSGSDEPFSHLLKHGFAAVDQCKRSGEAARL
                     ISGGAPDEFLIRALKRSIAQSKSRMEQGQTFLATVASSAPFVGLFGTVWGIYHALLAI
                     GITGQGTLDKVAGPVGEALIMTAIGLAVAIPAAVAYNVFTRANRNSVTALNGFADDIL
                     NLFATGMSIRGEAATGSASVANQPAIHTLRVQTGP"
     gene            complement(24705..>25154)
                     /locus_tag="PKI23_04625"
     CDS             complement(24705..>25154)
                     /locus_tag="PKI23_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNN36259.1"
                     /translation="PKKVVSRVKPSQKPSQVAAPSETPAVAETAPVATVPAEATPPSP
                     PIAVPPAAKSELTEPRFDADYLDNPKPAYPSISRRLGEQGRVLLRVQVDERGLAREVE
                     LHTSSGHPRLDTIALQTVRRWKFVPARLGEQKVAATVLVPIVFSLKG"
BASE COUNT         5287 a         6669 c         7759 g         5439 t
ORIGIN      
        1 aacatctgga gcatctcttc atcaaattgg gggtcgagac acgcactgcc gcagccgccg
       61 tcgcggtgca gggactgcat ggcgtgggcc gctgacggga cgaatttctg caatggtgga
      121 aatcggggct tcgtcggttt tgtgccccgc atcacgtcta ctttgcatgg agcgctctat
      181 actttgtcca ttcaggcaat tattacaggg agtttgtggt gatcgcgctg cgactgaaac
      241 agtttctgga tgagcaggcc gtttcctatc ggctgatcga gcagccgcag ggccgttcga
      301 tggaagaggt ggccaggctg gccctgatcg acccggtgct ggtggcacgg gccgagctgt
      361 ttttcgatgg cacgaccctg ttgatggtgg tctctccgct gacttatcag atcgacatgc
      421 agcgactcga ccggctggtc ggacgtccgt tgactgcact gtcaccgcgc aattccgaac
      481 ggttctttcc tgactgcgaa ccgggatgct atccggcgct gggtgccagc tatggcctgc
      541 ccgtggtcat ggatgccttt ctggcgcggt ttcccaaggt gtacatcgcc acgggccacc
      601 atacgggtct gatcgaactc gaaggggatc agtttcgtcg gctgcatggt gcggtgctgg
      661 aaggagattt caccctcaag ccggcgcgtg tggcgccgga gccgacggag agtgaagcgg
      721 ctccgatcga tcatgagcga ctcaagcgca agatcgaacg cagcggacgg ttgccagcca
      781 tgccggatct ggcactgcgc atcctgcaac tggtgcgcga cccggagttc tccaccgccg
      841 aactggggcg gctggtcgct caggatcccg ccatctccgc ccaggtgatg aagaccgcct
      901 ctgcttcggc ctatgcctat cagggcaaga tcaacagcat cgatgtcgcc atcgtgcggg
      961 tgttgggcta ccggatggtg acccacattg cggtcggcat ggcggtctcc agaagttttc
     1021 gcattcccga ggaggggccg ctcgggctga aaagattctg gctgcgggcg atcttctgcg
     1081 ccaccgcctg tcagatgatt gcacaacgat tgccgaagac gctgggtgtc gacgccgggc
     1141 tggcctacct cacggggctg ctgcatcagt tcggcctgct gatgctgggc catctgtttc
     1201 tgcctgagct gcgcatgctt ggcaaactgg ccgaagtctc gcccgaggag ccgctccatc
     1261 agttggaacg gcgcattctc agtgaagggc tgggtgccga cgcgctgcgg cttggccatg
     1321 cccgtctggg cgcatggatg ctgcagctgt ggaacatgcc cgccgaactg caggcggtgg
     1381 tcgcccacta cgaagcgcgt gactatgccg gcgaacatgc gctctaccat gcaaccctgc
     1441 tgctggctac cgacatgttt tccgcgcatc tgctggggca cgagcagctc ggcccggcat
     1501 cgaccgcgcc gctggcacgg ctcggtcttt cgtcgcagca gatcaacgac atccacagct
     1561 cgatgctcga tcagatcacg ctgatcgaag agctcggcgc ggccctcgcc gcctgaattc
     1621 agggtgattc agcgcctgtt tcaagcaatc gcctgacggg tgcataggag cgccgatgga
     1681 tcggcgtggg gcccaaccgc tgcagggcgg cgagatgggc agcggtggga taccccttgt
     1741 gccgcgccag gccataacct ggatagtact gatcgagctg gcgcatctcg cggtcacgcg
     1801 tcaccttggc cagaatcgag gccgccatga tcgccggaat cagacgatct ccctgaacga
     1861 ctgcttcacc ttggcaacag agctgcccgg gcagccggtt gccatcgatc agcgccagtg
     1921 tcggtgcggg atcgagcagt gccaccgccc gcgccagacc gatcatgcgg gcgtgaagga
     1981 tgttgtgacg atcaatctcc tcgaccgtga cccgtgccac cgcgaaggcg atggcatggc
     2041 gcccgatctg ttcggcgagc tgttcgcgac gctgttcgct cagtcgcttg gagtcatcga
     2101 gaccggacag tggctggtcg gggtcgagaa tcaccgcagc ggcaaacagg tcgccacaca
     2161 gcggtccggc accggcttca tcggcgccgg cgatccgggc ggccgcatgg ccctgcagca
     2221 gcgtttcgag tctcgatggc ggttggggca tcagggcgtt gcgctggcct tggcgagggc
     2281 ggcctcgatc gatgccacgt tctgtggccc cagcaattga atggtctgac ggcggtcggg
     2341 cgagatcaga taggtgaccg gcagcacggt cggacgctgg tagccgaact gctggatcgg
     2401 gtcggtgcgc agcacgcgga attcgatgcc gatcgcctgc gcctgtttgc gcagttcgtc
     2461 atcggcgagc tggtcaaagt tgacggcgaa gagcgccaac tggtcgctgc gctctttggc
     2521 caactggttc agctgaggca tctcttcgcg gcagggcgca caccagcttg cccagtagtt
     2581 gatcagcagc cactggttgg cgtgatcgct gaaacggccg ctgcggccgt tggcgtcgac
     2641 aaaatcgggc tgcatcccgc agccgacgag cagaagcgcg gtcagcagca gaaggcggag
     2701 aggccggcaa agccgctgac gagtgactat agttgaatga cccaatgcct ttcgcatcct
     2761 cttcagaccg cccatgaacc acatcaccca agcaagcccc actgccgcgc tgcaactgac
     2821 tgttccgctg ctgatctggg gcagccatga taacacgctc gatctgaccc aactgatgcg
     2881 ccagctcgat gatccggccg ggcgtcagcc agagcggctg atgagtgatg tcttcgatct
     2941 ggtcagacgg tccaaccgca tcccgatgga agatcaaagc cgtgcgttgc tgctcgactg
     3001 cgctggccgt tgtcagcagt ttctgctggt acagtaccgc gcagccagac ggcagattgg
     3061 tatggccgca agcagcaatg atgggcagtc cgagcggttg ctgcggctga ctgaagagct
     3121 tggttttggt tacaagcggt tgctgctgtc acagatcgaa accttcgcgc cggttgaaca
     3181 gcaggcagtg acattgcgcg gtgcgatcac cgcgctgagt cagcagtttc tgctctgcct
     3241 cgatctgcgc cgcgctcgct ccaagagcct gtggggggag ttccaccgac tgtttcacca
     3301 tgccgagcgg atcaaggtcg agaaccgtca gttcagggag gtcgacggtg ctgcgggcac
     3361 caatctgcat ggcctttacc tgcgcactgt gctgaccttc gccgctgatc cctatgcgct
     3421 gccggcaaat gagcactggc tgctgcaatc ctatctggcc cgctggtgca gcctggtgcg
     3481 cctggatcct gccatgcagc ccccttcatt cagtcagtcc agccggatgg cggtcgatct
     3541 gcgcgggctg gagatcggcc cgctgctcga gcaggtgcag cagcaccgtt ttcagctcgc
     3601 tgccggcgaa cagtccgtgc aggcgctcgg tttcgaggca caggtttcga ccgatcaggc
     3661 gctgcggcta ctcgaccacc tgctcgagcg ctggacacag aaacggcaac gtggcgccga
     3721 acggcatgcc tgccagacga cggtgacgct gacagttggc ttcatgtcga tctatcgctg
     3781 tctgcgtggg agtgtgaccg gtcagcaaga gacttcgacg cagttgcagt tcgatgcggt
     3841 catggagaat gaaagcgacg gcggggccgc attgcggttg ccaaccgact accggggtac
     3901 cctgccggca gtcggcgagc tggtgctgct ggccaagcgc gacggctttg acccggtgcg
     3961 gccgatgctg ggctgggtgc gctggcagca acgcagtccg cgtggcgaga tcagttttgg
     4021 catggagtat ctgcacagca cgacccagcc agtcgagctc gatcagttcg atggcagcac
     4081 actctcggcc tcgcagcgtc cggcgctgct gctggtgcgg gcacccggag agtcactgcc
     4141 attcagcctg atcgcccagc cgcacatcta ccgctccgat cgcaccgtgc gactggcttt
     4201 tgtgcagcgg atcgagacgc agcaggcggt ggccggacgg atgatcgccc gcagcggcag
     4261 ttgtgaccac tttcagctgg agggtgatct cgatgtcgat tcaaccttgc cggacgaggt
     4321 gatcgagatt gattacgatc cctttccgtt caacctcggt tccctctcct gaatggcgtg
     4381 tcagccagcc agcgagtgct tgaggatgtt gtcgaccagc ccttcgagct gcttgatggt
     4441 gttggcttcc ttgaccaggt gacagaacgg acggtacttg ccgatgactg aatcgcccgt
     4501 gttccagctt atctgaggtt ccgggttgag ccagatcacc cgcttggcgc gctgatagat
     4561 cagtttcatc acatccaggc gcggttcgcc atagttgttg cgggcatctc ccagaatgat
     4621 cacggtgctg cggtggtcga tgttgcgcag attgtcggag ttttcgatgc ggttctgctt
     4681 caggtcgcgc agcatctgac catagtcggt cgagccgccg ccggcctcct tgagcgccag
     4741 ttcgatggcc tgctcgaccg gatgctcctt gaagatgtcg ttgacatcga ccagatggct
     4801 cgagaaggca aaactgtcga tctgcatcag cacgtcgttc aggctgtaga gaaacagcag
     4861 cagaaagcgc gcataggcgc tcaccgaccc ggagacatcg cagatgcaga cgatgcgcgg
     4921 ccgttcgagg ttcttggtct tccagtaggt ttcgaacatc accccgtcgt aggccacgtt
     4981 gcggcgcatg gtcttgcgaa aatcgagcac cccgcgctgg gtctgcttgc gcttcttggc
     5041 atgggtgtcg ctcagcttct tcgccaaccg gcgaacgatc tcgtgcatgc ggtgaaagtc
     5101 gcgccgttcg atgttcgaca gccgggtgtt catcaggaac tcttcgcgca gccgcttgct
     5161 ggtgccgctg ccgtagagtg aaagttgctg ctcgacgaag ctgcgcacct cttcgagcag
     5221 cagtttgcgc acctgacgca gcagcgcggc ctgcgccttg ccgcgcggac aggtggtgtt
     5281 ggccagctcg gcgatctctt ggttgatctc ctgcatcccc atctccttga ggatcttctg
     5341 ggtgtagatc cccttctggg tgaagagcca gatgtcggtg acgcccaccg agcgggcagc
     5401 acgcttcatc tcattggcaa gggcgacccg gttgcgctcc atcaccatct tcaccagttc
     5461 ggacttgccc tcgtagtggc tggcaacctg ctcttcggcg gattggccag ggggttggct
     5521 ttcgttcgcc ggcgccttgg cggggctggc ggaactggcg gactcctcac cctgctgaaa
     5581 gctgttgaag gtgaagaact catcgaagca gtcgttgaaa cgggccttct catcgacgct
     5641 cttggccagg gtgaccgaga gcgccagctt caggatttcg cggtcgtcaa agccgatcag
     5701 ctcggccgtg cgactggcct cgatgctttc agccgccgag atggtgatgt cgtggctgcg
     5761 aacggccttg aagaaatcct gaagcatctg ttgcacggct tatcctccgg tcttggccat
     5821 gggttgctcg ctgtcggcga tcagtgcatc gagctgctcg ttgaccgctt cgatgtcatc
     5881 ctggaacttg agaatggtgt tgagcgtctc ctgaaccagc ttgggttcga gcgattcggc
     5941 atgcatcagc accagcaccc gtgcccagtc gatggtctcg ctgatcgagg gcagcttctt
     6001 cagatcgagc gcccgcacct ggtgaacaaa gctgaccaag tgctcgcgca gccgctcgga
     6061 gaggcccggg acccggcttt cgagaatccg ccgctccagc tcgggttcgg ggtaggggat
     6121 gctcaggtgc aggcagcgcc gcttcagtgc atcgctcatc tcccggttgt tgttgctggt
     6181 cagaaagacg atcggcttga cgcgggcctc gatggtgccg atctcgggga tcgacacctg
     6241 gaagtcggag agcacttcga gcagaaaggc ctcgaattca tggtcggact tgtcgatctc
     6301 atccaccagc agcacgcagc cgcgctcttc gtgcagcgct ttgaacagcg gtcgcggttc
     6361 gagaaactcc tcggagaaga acaggtcgtc ctgttggcgc agcttgccca tcgcctcggc
     6421 cagcgagtgg gtggtgccga gcagttcatt caacttgtcc ttcagcaatt gcgtgtagag
     6481 cagttgcttg ccgtacttcc actcatagag tgccttggac tcatccagcc cctcatagca
     6541 ctgcaggcgg atcagcggca catcgaggat cgacgcagtg gttttggcca gctcggtctt
     6601 gccgacgcca gcggggcctt cgaccagaat cggtttgtgc agattgaagg cgatgtagat
     6661 ggcggtggcg atctcgcggt tgcagatgta gcccgcgttg gaaaattcac gctcgatttc
     6721 ggcaaccgag gtgaagcgtt ggtcgtgcag ctctttcatg aaacatcctg tggttgagga
     6781 aagtggccgc aaccagccac cagcaaagtt tttgattgtc accgatccgc ttcggtcatg
     6841 gcaatgtcaa atttgctttg cgtaaagcct gactctcaaa cggcgcaagg gtcgcctcgg
     6901 gcaaaactat ttggtaggct cggcctgcct gacaacagcg ataaaaacag gctgatcgag
     6961 gatgcccccc gcgggtgtgg catcacactt ccggaagttg ccgggccgtg ctgcatggtt
     7021 cggcagggag gtgcatcatc catcagggag tgaacatgat gactttgcac aacttctggc
     7081 attgggccga gcagactcct gcgcgcaccg cagtcatcgg gccagacggt cgacaactca
     7141 cctttgccga actcgatgcc cgcgtcaacc agctctctca tgggttgcgg gcactgggtt
     7201 accgccgtgg cgatgcggtg gcggtggtga ttcccaatgg catcgaatgg ctggagatct
     7261 ttcttgccac gcttcagagc ggactctaca tggtggcgct caaccaccat ctggtcggtg
     7321 ccgaactcgc ttacatcatc gacaactgcg atgcgcgtgg cctgatcggg catcgccgtt
     7381 atggcgaggc gcttgcggct gccgtcgagt cgctcgattt cgatccggcg cagcgattcg
     7441 gcatcggcgg gttgccgggc tttcacgact atgaaagcct gttggcaggg cagcccatca
     7501 cgccaccggc cgaacgctcc tgcggcgcgc tgatgctcta cacctcgggc accaccggca
     7561 aacccaaggg ggtccggcgc aaattgcccg aagagtcgcc tgaagaagtg gccttcaagt
     7621 catcctttct ctgcctgctg ttcaatggca gcgtcggcag cggttctcat ctggtgcagg
     7681 gaccgctcta ccactccgga ccgagtggct tcggcatggc ggcgctgcag gccggacaga
     7741 gtctggtcat catggacaaa tggacggccg aagcggtgct ggcgttgatc gaccgtcacc
     7801 gcatcaccgc cacccacctg gtgcccaccc acttcgaacg gttgctcgat ctgcccgaag
     7861 cggtgcgggc gcgtcatgac ctctcctgtc tgacccatgt ggttcatgcg gcggcaccct
     7921 gtccggtggg cagcaagcag cggatgatcg actggtgggg accggtcatc cacgaactct
     7981 atggcgcaac cgaagggggt ggcacctcct gctcctcgca ggagtggctg cagcggccag
     8041 gcacggttgg caaggcctgg cccgtgtcgc aggtgaagat tctcgatgag tccggctgcg
     8101 agctgccggc gggtacaccg ggtctgatct acatgagtac agccattgcc cagttcgagt
     8161 accacaagga cagcgaaaag acgcagcgca gtcgccgtgg agaactcttc accaccggtg
     8221 acattggcta tctggatgag gagggctacc tctttctttg tgatcgtcaa tccgacatgg
     8281 tgatctccgg tggggtcaac atctatccgg ccgaaatcga gggcgcgctg ctgcagcacc
     8341 ccaaggtgcg cgatgtcgct gtttttggcg tgcctgatcc gcaatggggc gaagcgatca
     8401 aggcggtgat cgaagtgagc gacggggccg aaccgggcga gcggctcacc gatgagttga
     8461 aacagtttct tgaagggcgt ctggcccgtt acaaatggcc gaagtcattc gattatgtga
     8521 gcacactgcc gcgcaccgaa accggcaaat tgatgaagcg cacactgcgt gaaccctatt
     8581 gggccggtct ggagcgcaag gtgtaaccgc agcgtgcgcg ccattcattg atgaggagtg
     8641 catcatggaa aacgccgaat tcatcccgct ggcgctggct ggcgtctcct ttgtcgctgc
     8701 acatctgctg ccatcgagtc tggcgctgcg cccgcgtctg gtgcggatat tgggcgagaa
     8761 cggctatctt gccgcctaca cactgtttgc gttcgccacc ttcggctggc tctgccatgc
     8821 ctacgtgcag gcgccgcaca cggtgatgct gtggaatccc ggcgttttgc ggttcttgcc
     8881 gctgctgttg atgccggtcg cgttgtggtt catcgtctgc ggtgtcagcg gtcccaaccc
     8941 cagcagcgtc aggcaggagc agaagcttgc cgaggccgaa gtggccagcg gcatgttgcg
     9001 catcacccgc catccggtgc agtggggcat cgccctgttc gctctcggcc acctgctggc
     9061 caatgcagac cttgccgcgc tgctcttctt tggtgccttg ctgttgctgg ccacgctggg
     9121 catgtaccat atcgatcaac gcaaggcggc caccctgggc gagccttggc agcggtttgt
     9181 cgcagtgacc tccctgctcc cattgggtgc ggttctggcc gggcgcaacc gttttgtgat
     9241 cagtgagttg ggtggatggc ggctgctgtt tgccggagca ctctatctga tcctgcttct
     9301 cgcccaccct tgggtgatcg gggtttcacc tttttagccc aaaccgggaa cagtcgagga
     9361 gactgaaggt cgaaggcctg tcgagcgtcc atccagcgat gcgcaagtct ccgcaacagg
     9421 gctgatcggg ccgctgcacc gcgctcttcg catcgcatct catccccagc gagcagcaac
     9481 agtcatgaat gcatctctca gtcgattttc tgcctttttt tcacgggaat tggcaggagc
     9541 tctgctgatc ctcttcaccc tgtttctgca gggctgtggt gccggctaca ccgcagtcaa
     9601 aaagcgcgat ctggtgacgc agaccaagat gagtgactcc atctttctcg atccggtcgc
     9661 acccgaaaaa cgggtggtgt ttgtcgagat tcgcaacacc accgatcagc agttgcaggt
     9721 cgagcaagcg gtacggcaac ggctgagcgc acggggttat cgcctgaccg acaatccgga
     9781 tgaagccacc ttcctgttgc aggccaacat cctgcaggtg gggcgttccg acctgcgcgg
     9841 ttcgtccgac gccatctcct ccggcttcgg cggtgcggtg gccggtgcgg tattggccag
     9901 cgccaccggc agcagcaacc gtgaagcggc aggtgttggc gtgttggctg gagcagccag
     9961 tgttctgagt gatgcgatgg tcgatgacgt gctttacacc atcgtcaccg atctgcagtt
    10021 tcgccagcgg ttggcagcag gagagcaggt ttcgcagcag cagtcgacca cgctgaagca
    10081 gggcaccagc accgagctga aggagtccgt gacccccgcg cccacccagt ggaagagcta
    10141 tcgtacacgc atcgtcagca gcgccaacca ggcaaacctg aagcttgaga ccgcgttgcc
    10201 ggcgctcgag gccggactgg ttcaggcact gggcgggctc ttttgatgag actccagatg
    10261 cggccgtgca aagacgagtc gcgcaggagc aggtgtctgc acacttgcgg ttcgctcttg
    10321 aaatggtgtg tacgcgggac catattcttg tgagcggtca tcgattgtga gagactcaaa
    10381 aaaagctgtc atccgggcgg gcaggccgtt cggagcggcc tgatgggtgc tgctgattga
    10441 actcgcgtgc cgacccacgc cggacaccgt ccgggagttc gactgccacg gtttccttgc
    10501 tccatgccat aggcccggat gtccatcgcg aaagtggcgg aactggtaga cgcgctggat
    10561 ttaggttcca gtgtcgtaag acgtgagagt tcgagtctct cctttcgcac caaaaaatct
    10621 ttgctacgat tcaatctgtc acatgaggtt taggtatgca ggtttctgtt gaggctgttt
    10681 ctgttctggg acgtcgactc accattgatg ttccggtcga gcgcatcgag actgaagtca
    10741 gtgctcgtct gctcaaggca gccagaacca tgcgtcttga cggctttcgg cctggcaaag
    10801 taccgctcaa actggttcgc gaacgcattg gtgaaggggt ccgttccgaa gtgctgggcg
    10861 acgtgatcaa cgacagcctg aacaaggcga tcgtcgatca gaacctaatt ccggccgggc
    10921 gtccgatggt tgaggcgatc gatacagagc agccgcaaga cctgcggttt gtcgcgacct
    10981 tcgagatcta cccaaccgtt gatcttgccg atttttcagt gctgaatgtc cagcgcctca
    11041 gcgcagaggt gaccgaggcc gacgtggaca agatgataga gaccatccgc aagagcaatg
    11101 cggactggac ttccgtccag cgtgctgcgg ccaatggtga ccagttgaca gtcgatttcg
    11161 agggttttgt cgatggcgaa ccgctggagg ggggcagcgc cagcggtgcc aagatcgttc
    11221 tggggtcaca gcggatgatt cccggtttcg aggatgggtt gatcggcgcc gaagtcggtg
    11281 cagagcgaac ccttgacctc aagtttcccg agccctacca caacgcggcc gtggctggaa
    11341 aggatgcaag gttcaaggtc aaggtgcagg agatcgaaga gcagacgctg ccagaaatcg
    11401 gcaaggcgct gttcgaacgg gtcgaatttg atggcgacag tgttgaagcg ttccgggccg
    11461 aagtgcgcag caacatggag cgcgaaatga acgctgcgct gcgcagaatc accaaacagc
    11521 gagtgatgaa agcactcgaa gaggcgcact cattcgactt gccaggcgcc ttggtcggca
    11581 gcgaaatcca gcaaatgcgg catcagttgc tggagcaaat gggtcatcgg catgggcacg
    11641 actgcgacca tgaccatgaa agcgatttcc ccagcgaaat ttttgccgaa cgcgcccagc
    11701 agcgggtcaa aagagggctg attctggccg aaatcatcaa gggcgtgccg atcaagagtg
    11761 atccaagccg ggtgcgactg tttgtagagg agctggcttc cagctatcgg gagccagagc
    11821 gggtggttga gtggtactat cagaacccgg agatgatttc atcgatcgaa gccttggttc
    11881 ttgaagatca ggcaatcgac catgtgttgg cgaacacgca ggtgtccgat gaaacgacaa
    11941 cctacgaagc agtcattcga gaggcgtccg aagccgcgaa tggctaaagc gctacatgcg
    12001 gtggccacaa cgggtaagtg tgagccgccg atgtcctggc aatgatcacg gttttgcaaa
    12061 gttgagaaac agaagatgat tgaaaatgtt ggcttggtcc cgatggtagt cgagcaatcc
    12121 gcgcgcggcg agcgggccta tgacatctac tccaggttgc tcaaggagcg ggtgattttt
    12181 ctggtgggtc ccgtcgagga ccacatggcc aatctcatcg tggcgcagtt gctgtttctc
    12241 gaatcagaaa atccggacaa ggacattcac ctctacatca actctccggg tggatcggtg
    12301 acggccggac tctccatcta tgacaccatg cagttcatca agcccgatgt cagcaccatg
    12361 tgcatagggc aggctgcaag catgggtgca ttgctgctgt cgggcggtgc ggaaggaaag
    12421 cgctactgcc tgccacattc gcgcatgatg atccatcaac ccctgggcgg attccagggg
    12481 caggcttccg acattgaaat ccatgcgcgc gagattcttc tggtacgaga ccggttgaac
    12541 aagattcttg caaggcatac caaacaggac ctggaaacca ttgcaagaga tacggacaga
    12601 gacaatttca tgagcgcgat cgaagcggtc agttacggac tcatcgacca ggttcttgag
    12661 caccgggtcg attcattgaa atcagaaaaa tgagtttgca agccagccat tgcatggcga
    12721 gcaaagaatg gatttatatc ggccgatttt tttgagctag gatataccca tggttgatga
    12781 gcgcaacggc aggggcgatg agggcggcaa gctgctttat tgctcttttt gcgggaagag
    12841 ccagcatgaa gttcgaaagc tgattgctgg accttcagtt tttatctgcg atgagtgcgt
    12901 cgatctttgc aacgatatca ttcgtgaaga gatcgaggag agcagcaatg agtccagcgc
    12961 tgaaaagctt ccaactccaa gagacatcaa aaagaccctg gacgaatatg taattggtca
    13021 gtctttggca aagaaaatcc tttcggtcgc ggtctacaat cattacaagc ggcttggcca
    13081 caacccaaag gggaaagacg atgttgagct cgcaaaaagc aatattttgc tgatggggcc
    13141 aactggaagc gggaaaacgc ttcttgctga gacattggcg cgtctgttga atgttccatt
    13201 cacgattgcc gatgcaacga ccctgaccga agcaggttat gttggagaag atgtagagaa
    13261 catcattcag aagctcctgc aaaaatgtga ctatgatgtc gagcgggctc agcgaggcat
    13321 cgtatacatc gacgaaatcg acaaaatctc gcgaaaatcg gacaatccat cgattacgcg
    13381 cgatgtctcc ggtgaaggcg ttcagcaggc attgctcaag ctgatagaag gaacagtggc
    13441 ttcggtacca ccgcaaggtg ggcgtaagca tccgcagcag gaattcctcc aggttgacac
    13501 atcgaatatc ctgttcatct gcgggggcgc attttccggg ctcgacaaag tcattcgtga
    13561 ccgctcagag aagagtggaa tcgggttttc agcatcggta aagagcaagg aagacaagaa
    13621 aaatgttgga gaaaccctca agaaggtcga gccggacgat ctggtcaagt atggcttgat
    13681 tcctgagttt gtcggacgat tgccgatcat tgcaacgctc gaagagctcg atgaagctgc
    13741 cctgatcaag attcttactg agccgagaaa tgccttgacc aagcagtatg caaaactttt
    13801 tgacatggag catgttgaac tggacttccg cgaagatgcg cttagagccg tggccaaaaa
    13861 ggccatggaa cgcaagacag gcgcaagagg attgagatcg atccttgaat ccatcctgct
    13921 ggagaccatg tataatctgc cgtcaatgaa aggtgtcaaa aaagtggtca tcgatgagaa
    13981 tgttgtcgtt gatgagtccg aaccaatatt gctttatgaa aatctggaac aacagcgtgc
    14041 tgctccagat cagtaaggga tgaatagaga aaaagtgggg acggatgtcc cccttttttt
    14101 gttataaagg tcaaaaaaag atctgtggtt taattgagct tgatgattga ctgtggggat
    14161 agatagatga aatccgacca ggaagatact ttgattccag tgctgccttt gcgcgatgtg
    14221 gtggtctatc cgggaatggt tattccgctg tttgtcggaa gggcgcgttc aatcaaggcg
    14281 ctggaggcgg cagctgcttc agaagacaaa aaaattcttc tggttgcgca gagaaaccca
    14341 tcgatcgatg agccagagga agatgatgtt tatcgattcg gaacagtatc gacagtactg
    14401 caatcgataa aactccctga tggaacgatg aaagttcttg ttgaagggga gcagcgaagc
    14461 gaaataaaaa gcttcatgaa ggctgttgac tttttttccg cggtctcttc ccagcgctat
    14521 ccgaaagaga tctctccgcg gactgaagag ctatttcgga aatcgctctt ggagcagttt
    14581 gatcgctacg cacaggtgaa caaaaaaatc cccgcggagg tgacaagttc gttggcggat
    14641 atttcggaag tcgggcgttt gtctgacgct attgccgcac atatttctct ggagctcaat
    14701 cagaaacagg aacttcttga gctggttgaa ttgagcgaaa ggatagaaca gttgatggcg
    14761 atcatggagg cagaaattga tctccagaag atcgacaaaa agataaaaac acgcgtcaag
    14821 cagcagatgg agaaaagcca aagagagtat tatctcaatg aacagatgaa agccatccag
    14881 aaagagatgg gagagctcga gaattccgtc agcgagatcg agcagttgga gaagagagtc
    14941 tccgctgcaa aaatgcctgc ttatgcgcga gaaaaggtta aaagcgagct gaataaactc
    15001 agatcgatgt caccgatgtc ggcagaggca tcggttgttc gtggttatat cgactggatg
    15061 ataaacattc catggagcaa aagaagcaag gttgcaaatg atctgggtgc tgcgcgtaac
    15121 gtcctggatc acgaccatta tggcctcgac gaagtcaagc ggcggatcct ggagtttttg
    15181 gcagtgcaaa agcgcgtccg ctcattaaaa gggccggtac tctgccttgt tggtccgcca
    15241 ggcgtcggaa agacttcgct aggagaatcg attgcacgag ccacaaatcg taaattcgtg
    15301 cgaatggcat tgggtggagt cagagatgaa gcagaaatac gaggtcatcg ccgtacctat
    15361 gtcggctcca tgccgggaaa actattgcaa aagatggtga aggctggcgt taagaacccg
    15421 ctctttatgc tggatgaaat tgacaagatg ggaatggact atcgcggcga ccctgcctcg
    15481 gctctgcttg aggttcttga cccggagcaa aatcaccttt tcaatgatca ttatatcgaa
    15541 gtcgattatg atctttctga agtgatgttc atatgtacct ctaacagcat gaatattccg
    15601 ccggcattgc tggatagaat ggagattatt cagcttccgg gttacacaga agaagagaaa
    15661 atcagcattg ctgagcgcca tctgttgccg aaacagatga aggcgagtgg gctcaaagcg
    15721 aatgaattga cgattacaac ggcaggatta aaggagatca ttcgccacta tacgcgtgaa
    15781 gcgggcgtaa ggagtcttga gcggcagatc gcgaaaattt ctcgaaaggt catttatgag
    15841 agtgaaattt caacagagaa gagagatcca accaagcttg atgctgacgg cgtggagcat
    15901 tttcttgggg tgcaaaagtt tcgttttggt cgagctgaag aaaaggatca aattggccaa
    15961 gtgagcggtc tggcctggac ccaagtgggt ggagaaattc tgaccattga agctgcgtct
    16021 gttcctggga agggaaaaca gctctataca ggctctttgg gcgatgtcat gcaagagtcg
    16081 atacaggctg ctttcaccgt tgtcagaagc agagccaagg catttggaat ttccgaagat
    16141 ttctacgaaa agtgcgatgt gcatatccac gttccggaag gggcaacacc caaagatggt
    16201 cccagtgcgg gaatagggat gtgtgtggcg ctggtttcag ttttgacaac cattccggtt
    16261 cgcgcggatg tggcgatgac gggtgagatc accttgcgag gacaggtgct tgccattgga
    16321 gggctcaagg aaaaactcct ggctgcgcac agagctggga tccgtacagt tatcatacct
    16381 tttgacaatc agaaggactt gaaagaaatt ccggataata tcaagtccga gttggaggtc
    16441 aggccggtac ggtggatcga tgaagttttg caaattgcat tgacgaggca gccattgccc
    16501 ttgtccaatg aaaatagaga ggatgaaatt caggatccgg taattaaaaa ggcgaagagg
    16561 gtggataaga aagcgatcag gccacattga attctgtctt gtgttgcgga cgttacaccg
    16621 ctgtaaagct atgaagagtt gtatgtatga ataaatctgt tctaattgat tcggttgccg
    16681 tggctgctgg ggtttccaaa gcggcagctt tcagaactct ggatgcgatg acagagctgc
    16741 tcgcagagaa gctgagaaac ggtgagcaag taacgttgcc cgaatttggt gttttcaagg
    16801 tcgggcgccg aatgaaaagc gaggaaattg atccagaaat gcacgaatca tgttattttc
    16861 aatcgaagaa taccccatca ttcaaggtga gtagagaact taaaaatata ttgagttctc
    16921 gatagtcgat tttgatcata taatcctaat tatttttgaa gcgatttggt tatgttgcag
    16981 aatatcagag acggcttgag cggaattgtc tcgaaagcaa tagtcggatt cattgtattg
    17041 acctttgtgt tttttggcat tgagaccttg gtgcagtata agtcggaatc cgtgattgcg
    17101 actgtgaatg gattggatat tcctgagagc actttcaggc atgctgttga acttgaaaaa
    17161 agaaatataa tctatcgaaa ccaaggggtc gttccaaaga ttgatgaggc cgtgcttgct
    17221 tcgcaggttc ttgacaaatt gattgatata aagcttcttt ctggttatgc ggagggtttg
    17281 tctgttgctg cgcaaaaacc gcaaattgat caattgatca ctgctcaagc agagtttcaa
    17341 gttaatggaa aatttgatcc ggcggttttc aagcaggtga ttgagagctc cggttttact
    17401 ccgcagcgtt attatgaaaa tctcaagacc gatttcgttc tgaatcaact gagtcaagcg
    17461 tacctttata gtagctttca gtcaaaaaat gattggcagg atgtggcggc gctcgtacag
    17521 cagcgccgct cattttcgac tctggatatc ccgatcgaac gctttttcaa ggatattgat
    17581 aaagatgacc cagccataaa gagctattat gaggctcact cggatcagtt caaaactgat
    17641 gaggcagtta cagttgaata tattgagttg acgattgatc aggcaatgag ggaagttgaa
    17701 atatcgaatg atgaagtgaa agcgcgttac gagaaggaag tcgagacact ttctgcgagg
    17761 aagcgcttgc atgcagcgca tatcctggtc cgtgcttcgt cagaaaatga tctgaatgca
    17821 aaaacgaaga tcgataagat caatgaagaa ctgcgcagcg gtgctgattt ttcagaactt
    17881 gcaaaaaaat actctgaaga taaaggcagt gctggaaatg gtggtgatgt gggagcaacg
    17941 gatggaagcg tctttccgcc tgaatttgag catgctctgc aaaatcttca ggtgggagaa
    18001 atctcttcac caatcaagac cgaggctggc tatcacctga tccgcctgct cgaaaagagt
    18061 gattcgaaaa ttccgaaact ggatgaactt gcgagcagtc tgcatacaga attgagcagg
    18121 gaacgtgcac gactcattct gaaggagaaa gtggaaaatc tgtcgactgt ggtttttgag
    18181 tctcctgatt tgggtgaacc agcggttcgg ttcgggcttt caaagcaagt ggcgggtccc
    18241 tttggaaggg atcatgggga aggaatcgct gccaatctca aggtgcgaga gcaggcattt
    18301 tctgaggcgg tgctgtcatc tggacaaaac agcgaggtga tagagcttga tgagggacac
    18361 tatgtggcac ttcgcgtgtt gcgccacgat ccacagcgtc aattgagttt gaatgaagca
    18421 gcagagaaaa ttcgtaatat tgtctcaaga gaagctgcaa ggacacatgc ggaaaatgtg
    18481 gcgaaaaagt ccattgacga gttgcgctta ggaaaaccca ttgatgaagt ggccaagaac
    18541 gcgggtttga aatttaggca ttattcgaag gtgctgagaa gtggtgatca agaccttgat
    18601 ccacaacttc ttcaatcggt ttttcgtgcg gaaattccag tttcaggtag tgtagagatc
    18661 ggctcagcga tgctcggtga tggcgatcaa gtcgtttttt tgctgaccga ggtgatatcg
    18721 ggagctcatg atgaattgac agctgaacag caagcccagt tggtgaattt tttggcgttt
    18781 ggatctggaa aacgtgattt ctcttctgtt caaagacagc tcaagaccca ggctgatatc
    18841 gtgaaaaaca acttttgacc tggcagatcg cttttagata tttgttttcc acatggaaaa
    18901 atgtgatccg gtatcaaatc acacgctaca tgggagatgg caatgaacga tttgcctatg
    18961 gctggggagt ggtactgtat tatcggtcag gaagagcctt ttcaagtggt ggccattgac
    19021 gaagagagtg gcctggtcga agttcagcat ctcgatggcg atatcgaaga gtatgagatc
    19081 gaagagtggg aggctcttga tctggaggag atcgagcctc cggaagacag ctctgcgcct
    19141 tacgataact attcttatga tgaagacgag gagggagagg agattccaga cggattctct
    19201 ttgttaggtg aggaggaatg agcttttcat ttgagagtat agatgtcgcg gtctgtcgca
    19261 tcccggacaa tcatatagac ccacgcaggt atccgttgct catggcgcgc tcgataatgt
    19321 atggcgtgat cgatgaactc gaagccggga ggcggagcgt gtcaaccttt tttcaacgga
    19381 tcggcgcttt ttttatgtga caactttggg tggctcgacg ctgacttttc tcaagggaca
    19441 accgcgcgat cggccaggca gtgtggtcca atctcactga gccgggcgca gccagtcaat
    19501 gccatggtca tctccaattc gtcgcgcagc agccgcaccg catgggcaac acccagggcg
    19561 ccattgcagg cgagtcccca gatgaaaggg cgtccgatca gcactgcggt ggcgccgagc
    19621 gcgagggcga tgaagaggtc gtggccactg cggatgccgc tgtcgaacag gatcggcata
    19681 cgtccggcga tgcgcgcgct gattgccggc agcaggtcga tgctggccgg cgcaccatcg
    19741 agcacccgtc caccatggtt cgatacgatg atgccgtcgc agccgagatt cagcgcctga
    19801 tcggcatcgt ccggatgggt gatgcccttg agcagcagtg gcagtttggt ctgctgacgc
    19861 agccaggtga catcgttcca ggtgggggcc tgtttcatcc agccatcgaa cacccggctc
    19921 aaagccggat cgactggctc cggtggtgcg accgcatcga ggttgacggc gctgacccca
    19981 ttgggcagtt gcaagctgct gcgtttgatc ggggcgtcga tggtcagcac cacgaccgaa
    20041 aaacccgcgg cgctggcccg ctgcagcaag cgcagcgtcc gttcccggtt gccctgccag
    20101 tagagctgga accaggggcg gccctgttcg ccgtgaccgg ctttggcgat ctgctcaaag
    20161 ggttggctgg ccagactgct gaccagcatc tgcccacctt gggcagtggc ggccagggca
    20221 ctggcacact ccccatcggc atggaacagc ctctggtagg cgatgggtgc gagcagaatc
    20281 ggatggtcca actgctggcc gtagagttgc agccgggtgt ggccaccgct cagatcacgc
    20341 agtgggcgtg gccacagtcg aaccgccgcc agcgcgcgcg cattggcgtc gatggcctga
    20401 tcactgctgc cctcctgcaa ataacgccag agcggttcgc caagacgggc ccgcgccaac
    20461 ggcaagtagt cgcagagccg ctcgatggct gacacttcaa ctctccgccc agcggcgcag
    20521 caggttgtgg tagaggccgg tcagcctgac gacggcatct gactccccca tctcgctgcg
    20581 cagtgtcagc aaggccatgt ccatctcata gagcaggcgg cgctggccag ggtcgcgcac
    20641 catgctctgg atgaacagaa aacaggcgag tctctcaccg cgcgtgacgg ccgaaaccgc
    20701 atggatcgac gaagaggggt agagcagcag gctgccggcc tcgagcttga aaccgtggtt
    20761 gccgaaggtg tcctcgatgg tcagtacgcc gccgtcgtac tgttcgggat cggacagaaa
    20821 cagcgtggcc gacagatcgg cgcgcagata gtggccaggc tgctgcgcaa tgcgctgcat
    20881 ggcgttgtcg gtgtgaaaac catagctgtt ggtgagatcg ccatagcggt tgaacagcgg
    20941 tggcagaatc ttcagtggca gtgccgcagt gaaaaacagc ggattgcggg tgagcgcatt
    21001 cagcacgatc tggcgcagtg ccggcaactg ttcggcccct tcggacagtt gctggttgtt
    21061 cttggtcact gctgcctgga tgccggcggt ttcgagcccg ctgcgccagt gggcgcgctc
    21121 cagcagatcg cgtgaggtgg ccagctcatc ggcggtcaac aggttgtcga tggtgatgag
    21181 catggccgcc tgatccgctc agaacttgag ctcgccagtc agaatggcga tgcgccgtgt
    21241 gcccggaacc gtgaacacgc cgttgtcata gagggcatca tagtagagct gattgaacag
    21301 gttcttgaca ttgagccgtg ccgaccagcg tggctgttcg taggagagca tcagatccca
    21361 gcgcgcatag gccggtgctg tgttggggtt gaacttgctg ctgccgggca gggtcggaat
    21421 cgcaccggta ccgcttggat tgtaaccgta gcgctccccc ttcgcctcga ccccaccgcc
    21481 gatcttccat tgcggcgtca acttgtagct gctccacagg ttgaaggtgt agggcggggt
    21541 gttgcgtggc cgtttgccgg cataggccgg gttggccacg gtgatcgctc cggtggtggc
    21601 attcacgttt tcggccactt ccagaatttc ggcctccatc agcgccagcc ccgagaagat
    21661 ctcccagcgc tcattgatgc ggccggccgc ttcgagttcg aggccgcggg tgcgtcgttc
    21721 cttggtcagg atggccgctg tcgattcgag atcggtgttg cgctcccact ccttggtggc
    21781 ttcgtacagg gcggcgcgaa aggccagatc accctcgaac cagagccatt tgctgccaag
    21841 ttcgaccact tcgctgcgtt cggccggcag tggggtcacg gtgagttgat aaaggtcggc
    21901 ggtcgggctg aacgaatcac tccagctcag atagtagtga ttttcggtgg tcgggtgaaa
    21961 cgaaagcgcg ctgcgatagc tgttttcccc ataggcgagc gaaggtgagg tctgactgct
    22021 gtaatcggca tccatctcat cgcggcgaac gccgagggtg aacttccact gcgggatgaa
    22081 ttcgaccgtg tcctgggcat agaacgcata ggagtccgag tcgaaattga ccggcgcggc
    22141 ggttggattg gggtcatagg ggcggtagta gggcgggttg ccggcagtgg tgccgccgag
    22201 gttgcgcaac gtggtgcgat ggctgttctc attgaggtat tcgacaccgg tcaggaacag
    22261 atgcgccatg cccagtgcac tgaaggcggt gctgtagtcc gattgcagcg tgacggtctc
    22321 atagtcggcg gtgcgggtcg gaccgccatt ggccacgctg ccattgagca ccagcgcctg
    22381 cggattgggc ggggaggtca ggctcggcgt gcgtgcccag tagctgcgtt cgtagtcggc
    22441 cagccgcagt tgcgtgcgca actggctgtc gggtgagaag cggtactcgt tcaccagggt
    22501 ggtgatctga gtgttgctct catcgaaggt ggcatcggtg ccccagaagg tttcggccga
    22561 gaaagccttg gtcggcttgc gcgtgatcgg atcgaacgga attccgtaat cgggattgtc
    22621 gttggtcttc agccagtagt ggttgatcca gaactggttg tcgctgtaga gattgagccc
    22681 gagactgagg ccgacccctt cgcggtcgat ctccggttca ttgccagtgg tcggattgct
    22741 gcgccaactg ccctcgttgc gctgcatcag gttgacccgc agtgccgcgt cttcgctgag
    22801 cggcttgttc aggtcggcgg tcgcctcgcg gtagttctcg ccaccgacac tgccggtgag
    22861 ctcatactgc gccttgcgca gcggcgtctt gctgacctga ttgatgacgc caccggcttg
    22921 gccgcggccg aacagcatcg ccccggcgcc gcgcagcaca tcgatctgct cgtagttgaa
    22981 ggtctcgcgg ttgtactgcg cggtgtcgcg aatgccatcg agatacatgt caccaaaggt
    23041 gtagaaaccg cgcaggttca tgttgtcgcc cgaacgacca ccttcggcgg cgttgaaggt
    23101 gaggcccgac acgttgcgca gcgcctcgcg cagcgaacca acctgctgct cctccatcag
    23161 gctgttggtg atggtggtga cagcctgcgg aatttcgtgt ggatcttgca ggagcttgcc
    23221 gacacgggtg gccgtggcgc ggtaaccatc gggctgttcg accgccgcct ccacattgac
    23281 cgtggataac tgctgcgccg tttcagcggc cttgtcgtcg acagccatgg ccgacaggct
    23341 ggccatgccg gccagcatca tcgcgccaag cggcagcagc ggtaccgtgg ccgggtttcg
    23401 ctccagctcg gtttttttct caatgtagct catagaggac tcctcgatgg acagtgtgct
    23461 ggctggctcg aggcatctct ggctggctgc gatgggtgga atgaagcggt ttcactggcg
    23521 cctgggttcg gtgacgaagg cgattcggtt caatccatgc tgggcggcgg tcgacatgac
    23581 ctccgcaacc ttctcgtagg cagtggtgcg ctcggcacgc agatgcagct ccggctgtgg
    23641 cgagcgcgcg gcggcagcct gcaaccgcag gtcgagctcc tgcgcggcga tggcttcacc
    23701 attccagaac agctgtccag cgccatcgat ggccagctgg atgttctccg ggggcgtcag
    23761 tgtcggtgtg gaggatgcct tgggcagatc gaccttgacc gagtgggtca gcagtggtgc
    23821 ggtgacgatg aagatcacca gcagcaccag catgatgtcg atcagcggaa tcatgttgat
    23881 ctcggcaagt gccgaatcgc catcttcctc gaagggtgaa gcggagtgca tgggctgtct
    23941 ctatggaccg gtctgcacac gcagcgtgtg aattgccggc tggttcgcga ccgatgcgga
    24001 cccggtggct gcttcgccgc ggatgctcat gccagtggcg aacaggttca agatgtcatc
    24061 ggcaaaacca ttcagcgccg tgacactgtt gcgattggca cgggtgaaga cgttgtaggc
    24121 cactgcggcg ggaatggcga cggcaaggcc gatggcggtc atgatcagtg cctcgccgac
    24181 cggtccggcc accttgtcca gggtgccctg accggtgatg ccaatggcca gcagggcgtg
    24241 gtagatgccc cacaccgtcc cgaacaggcc gacgaagggc gcggacgaag cgacggtggc
    24301 gagaaaggtc tgtccctgct ccatgcggct tttgctctgg gcaatgctgc gtttcaacgc
    24361 cctgatcaga aactcgtccg gcgcgccacc gctgatcagg cgagcagcct cgcctgatct
    24421 tttgcactga tcgacggcgg caaagccatg tttcagcaga tgtgaaaagg gttcgtcgct
    24481 gccgcttccg tcgcagagcc gggtcagggt atccagattt tcggcctgcc agaaatggtc
    24541 gagaaaggtc cggcctctgc gctgcattgc cagcaaccgc accgttttgg tcacgatcag
    24601 ataccagctg ccgatcgaca tcagcgtcat cagcagcagg atgaaacgag caacgacatc
    24661 actgttggca acgaagtgaa caaagccgag agaattttcc atgctcaacc cttgagtgag
    24721 aaaacgatcg gtaccagaac cgttgcggcg accttctgct cccccaggcg ggctggtacg
    24781 aacttccagc gccgcacggt ttgcaacgcg atggtgtcga gccgtggatg gccgctgctg
    24841 gtgtggagtt caacctcgcg cgccagacca cgctcatcca cctgcacacg cagcaacact
    24901 cgcccctgtt cacccagtcg gcgcgaaatg ctggggtagg ccggcttggg attgtcgaga
    24961 tagtcggcgt cgaaacgcgg ctccgtcagt tcggattttg cagccggcgg gacggcaatc
    25021 ggcggtgacg gaggtgtggc ttcggcgggc acagtcgcca ccggagcggt ttccgccact
    25081 gccggggtct cactgggtgc agcgacctgg ctcggcttct gtgacggctt gactctgctg
    25141 acaacctttt ttgg
//
LOCUS       DAORCQ010000028        24934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1143_length_24934_cov_5.567989, whole genome shotgun sequence.
ACCESSION   DAORCQ010000028 DAORCQ010000000
VERSION     DAORCQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1143_length_24934_cov_5.567989"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKI23_04630"
     CDS             complement(<1..315)
                     /locus_tag="PKI23_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNN36260.1"
                     /translation="MKTVQKGFTLIELMIVVAIIGILAAVAIPAYQDYTIRAKAAELP
                     TFAGPAKMAVSEYMVSNNAAAWPADPTAAGFTTNITSKYVSSISYTAASGLIIVNGRA
                     TAL"
     gene            703..2418
                     /gene="pilB"
                     /locus_tag="PKI23_04635"
     CDS             703..2418
                     /gene="pilB"
                     /locus_tag="PKI23_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNN36261.1"
                     /translation="MASLSSIKLTGFSRRLVENGLLSEEVAIEAVSKSRQENVSLVSH
                     LVKRKLASSLDIAMAAAEEFGTPLLDLDAFDATMHPKRLIDVKLVRRHHVLPLYRRGN
                     RLYIALSDPTNLRALDEITFHTGITTQPVLVEEEKLGKSIDAYIDAQDDQLSSLGNLD
                     DASLEDLEISAGDERGEENDNSEVDEAPIVRFINKLLLDAIKLGSSDIHFEPYEKNYR
                     VRFRTDGILQEMARPPQNLAPRIAARLKVMSQMDISERRIPQDGRIKMKLSKTRAIDF
                     RVNTLPTLWGEKIVLRILDPSSAQMGIDALGYEEEQKQLYMRALEQPQGMILVTGPTG
                     SGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNPKVGLDFATALRSFLRQ
                     DPDVVMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPAFNIATS
                     VSLIIAQRLARRLCEHCKAPSSAPVETLLAEGFTPEQLETARIYTPVGCERCVRGYKG
                     RTGIYEVVPITPAISQLIMEEGNSLQIGAQAQKEGFRNLRQSGLRKVAQGITSLEEIN
                     RVTKE"
     gene            2422..3642
                     /locus_tag="PKI23_04640"
     CDS             2422..3642
                     /locus_tag="PKI23_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNN36262.1"
                     /translation="MATDIKSKQTFLWQGTDRRGNKSRGEIDGVSQALVKAQLRKQGI
                     NPTKVKKKPKPLFGGGKKKITPLDIAFFTRQLATMIKSGVPLVQAFEIVADGLDHIAF
                     KELVLTIRNDIAGGDSFAGAIRKHPLYFDDLYCSLIASGEDSGSLETMLDRVATYKEK
                     TEALKSKLKKAMKYPIAVVTVAVIVTGILLVKVVPQFQEIFTSFGAQLPAFTLFVIGL
                     SEIVQAYWFIVLAVIFALVAGFNRLMKTSESFRHNFERLTLKLPVAGPIIQQSALARY
                     ARTLSTTFAAGVPLISALESVADATGNIVYKEAVLKVRDDVSTGQQLQFSMRATGVFP
                     AMAVQMVAIGEEAGSLDEMLDKVATYYEDEVDTQVDSLTSLMEPIIMSVLGVLVGGLV
                     MAMYLPIFQLGNVV"
     gene            3660..4535
                     /locus_tag="PKI23_04645"
     CDS             3660..4535
                     /locus_tag="PKI23_04645"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNN36263.1"
                     /translation="MIELLAQHPGGLAALLFGLGALIGSFLNVVIHRLPKMMEQNWRH
                     ECQTLLELPVSAPPARFDLLWPNSHCPHCQTEIRPWHNIPLLSYLWLRGRCAHCQGRI
                     SLRYPLIELITALLSVTTFMLLWDGSQLWPPVAALLLTWTLIALTVIDLDHQLLPDSL
                     TLPLLWLGLLLNQASLFAAPADALYGAVAGYLSLWSFYWLFKLTTGKEGMGHGDFKLL
                     ALLGAWLGWRLLPLILILSSLIGALVGIALIALRGHDRQIPIPFGPFLAGAGWIALIW
                     GETLTRGYLAYLGIG"
     gene            4587..5204
                     /gene="coaE"
                     /locus_tag="PKI23_04650"
     CDS             4587..5204
                     /gene="coaE"
                     /locus_tag="PKI23_04650"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986240.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNN36264.1"
                     /translation="MFVIGLTGGIGSGKSAVSDRFAQHGITIVDADVASRVVVEPGRP
                     ALTRIAEHFGAELITSSGTLDRALLRSKVFSSEQERKWLEALLHPLIAEEIQSGLANS
                     RSPYTVLVSPLLLETTQHSYVDRILVVDVPVELQLRRTMARDNNSESQVRAIIAAQMA
                     RDTRRSRADDLISNDGSLEQLQQQVDALHQRYLALAAAKAAGAPA"
     gene            5201..5419
                     /locus_tag="PKI23_04655"
     CDS             5201..5419
                     /locus_tag="PKI23_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNN36265.1"
                     /translation="MNRSSADAPTRIHPCPVCRKPVAWNASSPQRPFCSRRCQLVDLG
                     TWASEGYQIPAEPSSALEQAPLEDEEQP"
     gene            complement(5432..6367)
                     /locus_tag="PKI23_04660"
     CDS             complement(5432..6367)
                     /locus_tag="PKI23_04660"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNN36266.1"
                     /translation="MTVEVVAAALFDRSGRVLACCRPAGKEFAGLWEFPGGKIEAGES
                     AEAALRRELHEELGIDATPGRRLIELPYRYPTKRVRIQLFAIDSFSGEPAPREGQGLR
                     WCRPEELLGLPLLSANRPLVQSLLLPQRLLITPSGLTETELLAGIEAALTRGIRLLQL
                     RLPEVKQSDYLRLAKLSLARCRAVGAKLLLHGEPELLQQVSADGLHLPSRKLLALRER
                     PLPATLLLGASCHSPAELAQAVAIGADYATLAPVQPTASHPAAQPIGWARFEQWVSAA
                     ALPVYALGGVGEADLPNVLQRGGQGVAAIRALWRG"
     gene            complement(6380..7600)
                     /gene="argJ"
                     /locus_tag="PKI23_04665"
     CDS             complement(6380..7600)
                     /gene="argJ"
                     /locus_tag="PKI23_04665"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNN36267.1"
                     /translation="MAVLPLPWPSIQPVAGVRLATAEAGIKRRGGADLTLFAFDAGSV
                     VSALFTRNAFRAAPVLLAETHLALAPPRYLLINSGNANAGTGPGGMAAARRCCELLAA
                     ETGCRPEEVLPFSTGVIGEPLPVARFETALPRLVTALAADGWEAAARAIMTTDTLPKG
                     SQREFMQGDKVVRVAGIAKGAGMIRPDMATMLAFLATDLEVSRPLLDTVLRQAVDASF
                     NRITIDGDTSTNDACVLAATGRSGATALTDLDSPLGRQFIDAVTEVCRELAQAIVRDG
                     EGATKFITIEVEQARSEAEALQVAYTVAHSPLVKTAFFASDPNWGRILAAVGRAGVEG
                     LDVGLINIDLDEVRIVSAGGRDPDYREERGQAVMDRAEIRVRIELGRGRVAQQIWTTD
                     LSHDYVRINAEYRT"
     gene            complement(7633..10353)
                     /gene="secA"
                     /locus_tag="PKI23_04670"
     CDS             complement(7633..10353)
                     /gene="secA"
                     /locus_tag="PKI23_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNN36268.1"
                     /translation="MLTAFVRRLVGTKNNRELKRMGKIVSRINGFEESCRGLDDSQLA
                     GKTAEFRQRLAAGETLDQLLPEAFAVVREAARRALGMRHFDVQLMGGITLHEGRIAEM
                     RTGEGKTLVATLPAYLNALAGKGVHLVTVNDYLAQRDANWMAPVYRFLGMSVGVILSR
                     QDQASKREAYAADITYGTNNEYGFDYLRDNMAFTVEERVQRALNFAIVDEVDSILIDE
                     ARTPLIISGQAEDSSELYRRINRLIPKLTRAVEASEVQPAVAGHYLVDEKLRQVELTE
                     EGHEHVEQLLTEAGLLGEGESLYSAANLGLLHHVHTALRAHVLFHRDVEYIVQNGQVI
                     LIDEHTGRTMPGRRLSEGLHQAIEAREGVAVQAESQTLASTTFQNYFRLYHKLSGMTG
                     TADTEAFELKEIYNLDVVVIPTNKPMLRIDHNDLVYLTLEEKYDAIIADIESCRAEGR
                     PVLVGTASIESSELLSSALKKRKIEHKVLNAKYHEQEAQIVAQAGQPGAVTIATNMAG
                     RGTDIVLGGNFEVELTQLGSVTPEQREAARQAWQLRHEQVLASGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLEDNLMRIFASERMRQFMQRLGMEQGEAIEHRMVS
                     NAIEKAQRKVEGRNFDIRKQLLEYDDVANEQRQIVYRQRRALLESDDISDTIVDFRKV
                     VVNEVIDEFIPPQSLEEQWNIAGLEQHLSADLALSLPLAQWLDQESDLHEATLRERIL
                     QALIEAYERKGQLLGTQMRLLEKQVMLQILDNLWKEHLATMDHLRQGIHLRAYAQRNP
                     KQEYKREAFELFQQLLSNLRHEVVKTLSRIEIVQEDIEEIERRRREEQARQALRYQHD
                     QASALQPPEEEAAEAAGAQPYVRVGEKVGRNAPCPCGSGKKYKQCHGKLD"
     gene            10529..10993
                     /locus_tag="PKI23_04675"
     CDS             10529..10993
                     /locus_tag="PKI23_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36269.1"
                     /translation="MPDPAHSRAQTPARFTPPRHSTLGDLYQQAETLAALQRLLAPLF
                     EQFGIDCDVAGYREGRLLLICADAARALRARHSGARLLLELRRLDRFRALQRIEWRVR
                     PVQQPARALPLPQRPKPASARLLSELAHNTADSRLRSALEKLAQPAKSADRG"
     gene            complement(11016..11927)
                     /gene="lpxC"
                     /locus_tag="PKI23_04680"
     CDS             complement(11016..11927)
                     /gene="lpxC"
                     /locus_tag="PKI23_04680"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNN36270.1"
                     /translation="MIRQRTLKNVIRATGVGLHSGKKVYLTLRPAPVDTGVIFCRTDL
                     EPMVLIPARAEHVGDTTLSTTLVKDGVRISTVEHLLSAMAGLGIDNAYIDINCAEVPI
                     MDGSAGPFIFLLQSAGIEEQARPKKFIRIKRKVEVRDGDKSAALYPFAGFKVAFTIDF
                     DHPVFRNRTQSAEVDFSSTSFVKEVSRARTFGFMSDIEQLRARDLALGGSVDNAVVVD
                     EYRILNEDGLRYEDEFVKHKILDAIGDLYLLGHGLIGRFVGHKSGHELNNRLIRTLLA
                     DKEAWELVTFDDAEQAPISYSRPLTVA"
     gene            complement(12195..13376)
                     /gene="ftsZ"
                     /locus_tag="PKI23_04685"
     CDS             complement(12195..13376)
                     /gene="ftsZ"
                     /locus_tag="PKI23_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNN36271.1"
                     /translation="MFELVDNVTQSPIIKVVGVGGGGGNAVNHMLSNDVEGVEFICAN
                     TDAQALKGLQARTLLQLGSGITKGLGAGASPDVGRQAAIEDRERIAEVLGGADMVFIT
                     AGMGGGTGTGGAPVVAQIARDLGILTVAVVTKPFTFEGRKRMQVAERGIEELAEHVDS
                     LITIPNEKLQLVLGRDVSLADAFNAANDVLLGAVQGIADLIMRPGQINVDFADVRTVM
                     SEMGIAMMGSGRASGANRAYEAAQRALHSPLLDDINLVGARGILVNITAGPSLSLGEF
                     TAVGETVAEFASESATVVVGTAIDPLMTDELRVTVVATGLGGAATQPTLTVIDTQRGE
                     EPREKSVLQPPVRPQRPLTAGSQALKPQRDQRPQPGLRELARQEIDYLDIPAFLRRQA
                     D"
     gene            complement(13452..14687)
                     /gene="ftsA"
                     /locus_tag="PKI23_04690"
     CDS             complement(13452..14687)
                     /gene="ftsA"
                     /locus_tag="PKI23_04690"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNN36272.1"
                     /translation="MVANSTNMIVALDIGTSKVVAMVGERSDNGVIEVIGIGTQRSRG
                     LKKGVVVNIESTVQSIQRAIEEAELMAGCQIHSVYVGIAGSHIRSLNSNGIVAIRERA
                     VNQSDVDRVLDAAQAVPVPADQKVLHILPQEYRIDDQEGIKEPLGMSGVRMEAKVHVV
                     TCAVNSAQNIEKCVRRCNLEIEEMILEQLASSYAVLTDDERDLGVCLVDIGGGTTDIA
                     IFHDGAIRHTGVIPIAGDQVTNDIAVALRTPVASAEEIKIKYACAHTQLARADEIIRV
                     PSVGDRPARELSRQALAEVVEPRYDELFTLVRAELQRSGYESMMTAGIVLTGGTAKME
                     GAVELAEEIFHMPVRLGVPQNVHGLADIIANPVYATAVGLLQHGFRQHEQRPAGGGSR
                     GPAVGNLLSRLKQWFQHNL"
     gene            complement(14733..15800)
                     /locus_tag="PKI23_04695"
     CDS             complement(14733..15800)
                     /locus_tag="PKI23_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNN36273.1"
                     /translation="MQSVLGRHAQPLKKAPAKSGRPSMATLQRREVPRKPARSARRDQ
                     GMARAVTPARRTAAAPTAARSRPVPSATGRMSAATGRIEPRPLSGRKVNQRIRPRVAQ
                     PPLHVRMQAVVLQGWQQLVDVGRALVWLLLRLGLVAAVVGIVWGAPRAIERLNPLIEQ
                     VAVHGTLNRLEQQHLQRQLEQVARGRFFTVDLEAVRAPLLGLPWIRAVEVKRIWPAAI
                     AVTVVEQQPVARWGERGLLNDAGEPFYPPSIEGYEHLPKLAGPTHLGKEILARYQQIR
                     QQLERVGVPCSGLGYDPAAGWWLQLDGEVKVQLGSRDLERRIDHLASLLRHSLKPALS
                     RVAAIDMRYPSAAAVRWREQN"
     gene            complement(15803..16741)
                     /locus_tag="PKI23_04700"
     CDS             complement(15803..16741)
                     /locus_tag="PKI23_04700"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNN36274.1"
                     /translation="MSTASVSLRERFGKVLLLHGGRSAEREISLRSGAAVLNALRAQG
                     IEVEVVDADERLVERLVDSRFDRAFIALHGRGGEDGSLQALLDLLDRPYTGSGALASA
                     LAMDKQMSKQMWRGLGLPTPPFMQVTAQTPWEEVLDRVGPVVAIKPNHEGSSIGISRV
                     GSAAEYAQALTLALRYDQQVLAERWIEGAEYTVSILHGEALPVIRVETSHTFYDFVAK
                     YQANDTRYLCPCGLAPADEQKVQSLALAAFAALGCRGWGRIDLLADAADNFWLLEANT
                     VPGLTDHSLVPMAAKAAGLSFEALVIDILATSERER"
     gene            complement(16738..18192)
                     /gene="murC"
                     /locus_tag="PKI23_04705"
     CDS             complement(16738..18192)
                     /gene="murC"
                     /locus_tag="PKI23_04705"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNN36275.1"
                     /translation="MVKLPEPVNRVAIDIPEMRRIRQIHFIGIGGVGMCGIAEVLLNQ
                     GYRISGSDLSAGENTRRLERLGATIGFGHEAGRISGADVVVYSSAVTSDNPELLAARA
                     QRLPVVSRAEMLAELMRFRHGIAVAGTHGKTTTTSLIASVLAKAGLDPTYVIGGRLNS
                     AGSNARLGASRYLVAEADESDASFLHLQPLVAVVTNIDLDHMETYGGDVNRLERTYLE
                     FLHNLPFYGLAVVCLDEPRLRALIPQIGRPVLTYGFSEEADFHAYDVVQQRNTVRFNV
                     RWPGGSGLPIELKMPGRHNVLNALATMAVAVDEGVDVEQIQQGLGEFAGVGRRFQLRG
                     EFALDSGGEVMVIDDYGHHPRELAVTIDAIRAGWPERRLVMLFQPHRYSRTRDLYEDF
                     VQVLSRPDLLLLLEVYAAGEPAIPGIDGRALCRSIRQRGPVEPLFVEDQATVPELLRQ
                     LLKGGDILLVQGAGNVGALANRIAASRLNWAEYR"
     gene            complement(18176..19279)
                     /gene="murG"
                     /locus_tag="PKI23_04710"
     CDS             complement(18176..19279)
                     /gene="murG"
                     /locus_tag="PKI23_04710"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNN36276.1"
                     /translation="MSQRSLTVLIMAGGTGGHVFPALAVADVLQSRGARIEWLGSRHG
                     IEQRLVPAAGFSLNCLDVGGLRGAGVRRWLGAPLQLVRAVWQAVRCIRRIAPDCVLGM
                     GGFAAGPGGLAAWLLRRPLLIHEQNAVAGVTNRLLRPLARRTFEGFPATFGGAGVEHV
                     GNPVRAEICALESPRQRFERRRADGEPLHLLILGGSLGAAALNELLPQALALLPAELR
                     PRVWHQAGAGKDEACRAGYRQAGVEARVEPFIDAMATAYGWADLVLCRAGALTLAELC
                     CAGVGGLLVPYPFAVDDHQSVNGRVVEQVGGAQLLPQATLDASTLAARLEPFVREPAR
                     ALALGMRIQTLARPQAAERMADCCQEVAHGQAA"
     gene            complement(19276..20463)
                     /gene="ftsW"
                     /locus_tag="PKI23_04715"
     CDS             complement(19276..20463)
                     /gene="ftsW"
                     /locus_tag="PKI23_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNN36277.1"
                     /translation="MSLSPPAMTTLWGNLLKRPVAADRVDLLLALPMLALALFGWVMV
                     TSASVDIDATNLGDAWYHSKRHGLWLLIGTGVLLLTRRVPLAWWERHGARLLLVAIVL
                     LVLVLVPGIGRKVNGSARWLGFGSVSVQTSEIAKAFILIYLSGYLVRHRVEVQQRWTG
                     FFNPMLILGLVIALLILEPDYGAVVVLMSTVLGLFFLAGMAVLRFSTLILGSIALGSL
                     LLVAQPYRLERIKAYIDPWADQYGSGYQLVQALIAFGRGEWFGLGLGNSVQKVFYLPE
                     AHTDFVFAVIAEELGLLGCLALVALFVMLIWRGLVIARNAECEGRLFGGYLAYGITLM
                     LAIQVLINLGVNTGLLPTKGLTLPLISYGGSSIVVTGFMLALLLRVETESGLPIRSEE
                     ESP"
     gene            complement(20454..21818)
                     /gene="murD"
                     /locus_tag="PKI23_04720"
     CDS             complement(20454..21818)
                     /gene="murD"
                     /locus_tag="PKI23_04720"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNN36278.1"
                     /translation="MHSVATTEKMQTLVVGLGATGLSVARFLARQGIHFAVADSRTMP
                     PGLEQLRTEQPGVACHLGPFDGALFSRFEQLVVSPGVPLGEPAIRQAAAAGARITGDI
                     ALFRAQTEGALVAVTGSNGKSTVTTLIGEMARRAGCRVVVGGNLGTPALELLEQGAAE
                     VVVLELSSFQLELVEQLDAEVALLLNLSEDHMDRYADLASYRAAKRRIFNGVRQVVTN
                     SDDPETRMDGATVRRWSYRLRRPASAQEFGLIEQGGRRYLAQGDEPWVATDALKLRGL
                     HNHGNMLAALAAGSAIGLPRAAMVEALLEFGGLPHRCQWIDRIDGVDYVDDSKATNVG
                     ATVAAIRGLAADLAGKLVLILGGVGKGADFSGLREPVRNHARAVVLMGVDGGEIAAAL
                     DPVTPSVQVQGIDEAVRQARDLAQTGDCVLLAPACASFDQFSGFAERGAAFQRAVREL
                     AACH"
     gene            complement(21822..22904)
                     /gene="mraY"
                     /locus_tag="PKI23_04725"
     CDS             complement(21822..22904)
                     /gene="mraY"
                     /locus_tag="PKI23_04725"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNN36279.1"
                     /translation="MLLWLTGQLAGYESLFGVFRYLTLRAILSVLTALAIALLAGPWV
                     IDRLAGLQIKQSIRTLGPATHLSKAGTPTMGGVLILLSMLTSTLLWADLSNRYILLLL
                     GVTLLFGAVGWVDDYRKVVQKNSIGLPARWKYFWQSLIGIAAALFLFQNATIAEQTQL
                     LLPFFKNVSINLGLLFIPLVYFVIVGTSNAVNLTDGLDGLAIMSTVMVGGALGLFAYV
                     AGHAGFADYLLIPHIKGAGEVLVFCAAIVGAGLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEIVLFIMGGVFVMETVSVILQVGYYKLTKRRIFRMAPLHHHYELKGWP
                     EPRVIVRFWIITLVLVLIGLATLKLR"
     gene            complement(22904..24268)
                     /gene="murF"
                     /locus_tag="PKI23_04730"
     CDS             complement(22904..24268)
                     /gene="murF"
                     /locus_tag="PKI23_04730"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNN36280.1"
                     /translation="MIGSFKLSDCCAACAGRLQGADVCFGSVCTDTRKLVAGDLFVAL
                     PGPQFDPHQFIDQAIARGAVAALVEQPIPYPLPHCQVSDSRLALGRLAELNRGRFTGE
                     LVAITGSCGKSTAKELTASILAEVAPTHATRGNLNNEIGVPLTLLELEAQHRYAVLEL
                     GANHLGEIAYTAALAKPKVAVITNAGEAHLEGFGSHAGIVAAKGEILDALPPDGVAVL
                     NRDDAGYDAWRERAAGRRVISFSLQGGSDAEITATDQVADADGRYRFELNTPVGQIAV
                     ALRLYGRHMVSNALAASAAAVAFGIDLTSIARGLSRVKPTAGRVMRLQGSSGAVVIDD
                     SYNASPTAVKAAIDLLCLQEGERILVLGQMAELGETTLELHRDIGRHARQHGVDQLVT
                     VGEVAAVAAETFGAGAVACGNVDAAIAWLKPRLQQGWVVLIKGSRSARLERVVEALSQ
                     QEVC"
     gene            complement(24265..>24934)
                     /locus_tag="PKI23_04735"
     CDS             complement(24265..>24934)
                     /locus_tag="PKI23_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNN36281.1"
                     /translation="HAEIRSIAQGELRAWLTTPWGCGELVTTLLGRFSLANLLAALAV
                     AAGRGFEFSALLAACRAVRPVEGRMERLGGAGRPTVVIDYAHTPAALQSVLEALREHC
                     DGALWCLFGCGGDRDRDKRPLMGAIAVRLADRVMVTDDNPRSESPVAITDQILSGVGA
                     AEVLVEHERSRAIEQVIGQAAAEDWILVAGKGHERYQEVAGQRHPCNDHAIVEAALQR
                     YRVA"
BASE COUNT         4454 a         8687 c         7655 g         4138 t
ORIGIN      
        1 cagcgcagtg gccctgccat tgacgatgat cagccctgag gcagccgtat agctgatgct
       61 gctgacatac ttcgaagtga tgttggtggt gaaccctgcc gccgtcggat cggccggcca
      121 agcggcggca ttgttcgaca ccatgtactc gctcactgcc atttttgccg gaccggcaaa
      181 ggtgggcagc tccgcggcct tggcgcggat ggtgtagtcc tgataggccg gaatcgccac
      241 cgccgccaga atgccgatga tcgccacgac gatcatcaat tcgatcaggg tgaaaccctt
      301 ctgtaccgtt ttcatgctca aagtctcctg aggtggattg gaatgccgga gtgcccgaca
      361 gattcgccat cgaagtatag tcaaacctgc cgggatgcaa ggatgacgac gcttgccatg
      421 gtcgcggctg gagcctaacg accatgccga accgaccaaa gcagttgcca tgccatgttt
      481 tgaagtgaaa ggatctcctg cattctttga gcaagatcac gctttatcgg ccagactgcc
      541 cggcatggcg ccctcgactg ccgcccgcat ggcccattgc ggcaacaggt gacaattctg
      601 gtcacccttg cgcccaaggt cattgggttg ccatcaccgc agcgtctact agaattgtcg
      661 gaaagctctt attcccctct acggccgaca ggaagttcca gcgtggcatc actctcttcg
      721 atcaaactga ccggcttctc acgccgtctg gtcgagaatg gcctgttgag cgaagaggtg
      781 gccatcgaag cggtcagcaa gtcacgtcag gagaatgtgt cgctggtgtc gcacctggtg
      841 aagcgcaagc tggccagcag cctcgacatc gcgatggcgg ccgccgagga gttcggcacg
      901 ccgctgctcg atctcgacgc cttcgacgcc accatgcatc cgaagcggct gatcgatgtc
      961 aagctggtcc gccgccacca tgtgctgcca ctctaccgcc gcggcaaccg gctctacatc
     1021 gcgctgtcgg acccgaccaa cctgcgtgcg ctcgacgaga tcacctttca caccggcatc
     1081 accacccagc cggtgctggt cgaggaggag aaactcggca agagcatcga tgcctacatc
     1141 gacgcccagg atgaccagct ctcctcgctc ggcaacctcg acgacgcctc gctcgaggat
     1201 ctcgaaatca gcgccggtga cgagcgcggc gaagagaacg acaattccga ggtcgacgaa
     1261 gcgccgatcg tgcgcttcat caacaagctg ctgctcgacg ccatcaagct cggctcttcg
     1321 gacatccact tcgaacccta cgaaaagaac taccgggtgc ggtttcgcac cgatggcatt
     1381 ctgcaggaga tggcacgtcc accgcagaac ctggcgccac gcatcgccgc acggctgaag
     1441 gtgatgtcgc agatggacat ctccgaacgc cgcatcccgc aggatggccg catcaagatg
     1501 aagctgtcga aaacccgcgc gatcgacttt cgggtcaaca ccctgccgac gctgtggggc
     1561 gagaagatcg tgctgcggat tctcgacccc agcagcgcgc agatgggcat cgacgcgctc
     1621 ggctatgagg aggagcagaa gcaactctat atgcgggcgc tcgaacagcc acaggggatg
     1681 attctggtca ccggcccgac cggcagcggc aagacggtgt cgctctatac cggcctcaac
     1741 atcctcaaca ccgaagagcg caacatctcc accgccgagg atccggtcga gatcaacctc
     1801 gaagggatca accaggtcca tgtcaacccg aaggtggggc tcgacttcgc caccgcgctg
     1861 cgctcgttcc tgcgccagga cccggacgtg gtgatggtgg gcgagatccg cgacctcgag
     1921 accgccgaaa tcgccatcaa ggcggcgcag accggtcacc tggtgctgtc aacgctgcac
     1981 accaacagcg cgcccgagac gctgacccga ctgcgcaaca tgggggtgcc ggccttcaac
     2041 atcgccacct cggtttcgct gatcatcgcc cagcggctgg cccgacgcct ctgcgagcac
     2101 tgcaaggcac ccagcagcgc accggtcgag acgctgctgg ccgaaggttt cacgcccgag
     2161 cagctcgaaa ccgcccgcat ctacaccccg gtcggctgcg agcgctgcgt gcgcggctac
     2221 aagggccgaa ccggaatcta tgaagtggtt cccatcacgc cagcgatcag ccaacttatc
     2281 atggaagagg gcaactccct gcagatcggc gcccaggcgc agaaggaggg gttccgcaac
     2341 ctgcggcagt ccggactgcg caaggttgcc cagggcatca cctcgctgga agagatcaac
     2401 cgcgtcacca aagagtaacc catggccacc gacatcaaat cgaagcagac ctttctctgg
     2461 cagggaaccg accgccgcgg caacaagtcc aggggcgaga tcgacggcgt cagccaggca
     2521 ctggtcaagg cgcagttgcg caagcagggc atcaacccga ccaaggtgaa gaagaagccc
     2581 aaaccgctgt tcggtggcgg caagaagaag atcacgccgc tcgacatcgc cttcttcacc
     2641 cgccaactgg cgacgatgat caaatcgggg gtgccgctgg tgcaggcctt cgagatcgtc
     2701 gccgatggcc tcgaccacat cgccttcaag gagctggtgc tgacgatccg caacgacatc
     2761 gccggcggcg acagcttcgc cggcgccatc cgcaagcatc cgctctactt tgacgacctc
     2821 tactgcagcc tgatcgcctc gggcgaggat tccggctcgc tcgaaaccat gctcgaccgg
     2881 gtggccacct acaaggagaa gaccgaggcg ctcaagtcga agctgaaaaa ggcgatgaaa
     2941 tatccgatcg cggtggtgac ggtcgcggtc atcgtcaccg gcatcctgct ggtcaaggtg
     3001 gtgccacagt tccaggaaat tttcaccagc ttcggtgccc agttgccggc cttcacgctg
     3061 ttcgtgatcg gcctgtcgga gatcgtgcag gcctactggt tcatcgtgct ggccgtgatc
     3121 ttcgccctcg tcgccggttt caaccggttg atgaagacct cggaatcgtt tcgccacaac
     3181 ttcgaacgcc tgacgctgaa gctgccggtg gccggaccga tcattcagca gtcggcgctg
     3241 gcccgctacg cccgcacgct ctccaccacc ttcgcggccg gcgtgccgct gatcagcgcg
     3301 ctggagtcgg tggccgacgc caccggcaac atcgtctaca aggaggcggt gctcaaggtg
     3361 cgtgacgatg tctccaccgg ccagcagttg cagttctcga tgcgcgccac cggcgtcttt
     3421 ccggcgatgg cggtgcagat ggtggcgatt ggcgaagagg ccggttcgct cgacgagatg
     3481 ctcgacaagg tggccaccta ctacgaggat gaagtcgaca cccaggtcga cagcctgacc
     3541 agcctgatgg agccgatcat catgtcggtg ctgggggtgc tggtcggcgg tctggtgatg
     3601 gcgatgtacc tgcccatctt ccagctcggc aacgtggtct gatcccgttg cccacagcgc
     3661 tgatcgaact gctggcccag catccgggcg ggctggccgc gctgctgttc ggccttggcg
     3721 cgctgatcgg cagctttctg aatgtggtga tccaccgcct gccgaagatg atggagcaaa
     3781 actggcgtca cgagtgccag accctgctgg aactgcccgt cagcgcgccg ccagcgcgct
     3841 tcgacctgct ctggcccaac tcgcactgcc cccactgcca gaccgaaatc cggccctggc
     3901 acaacatccc gctgctgagc tacctctggc tgcgcggccg ctgcgcccac tgccagggcc
     3961 gcatctcgct gcgttatccg ctgatcgagc tgatcaccgc gctgctgagc gtcaccacct
     4021 tcatgctgct gtgggatggc agccagctct ggccgcccgt cgcggcactg ctgctgacct
     4081 ggaccctgat tgccctgacc gtgatcgacc tcgaccacca actgctgccc gacagcctga
     4141 ccctgccgct gctctggctc ggcctgctgc tcaatcaggc ctcactgttt gccgcaccgg
     4201 cggatgcact gtatggcgcc gtcgccggct acctgtcact gtggagtttc tactggctgt
     4261 tcaagctgac gaccggcaag gaggggatgg gccatggcga cttcaagctg ctggcgctgc
     4321 tgggcgcctg gctcggctgg cggctgctgc cgctgatcct tatactttcg tcgctgatcg
     4381 gcgccctggt cggcatcgca ctgatcgcac tgcgcggcca tgaccggcag ataccgattc
     4441 cgttcggacc ctttctggcc ggcgccggct ggatcgcgct gatctggggc gagacgctga
     4501 cccgcggcta tctggcctac ctcggcatcg gctgacaaaa ctgcgcagcc cgtgcacctc
     4561 ctgcactcca acacggtaac ctcaggatgt ttgtgatcgg actcaccggc ggcatcggca
     4621 gcggcaagag cgccgtgtcg gaccgctttg cccagcatgg catcaccatc gtcgatgccg
     4681 acgtcgcctc ccgcgtggtg gtggaaccgg gtcgcccggc actgacgcgc attgccgagc
     4741 atttcggcgc cgaactgatc acatcctctg gcacgctcga ccgcgccctg ctgcgcagca
     4801 aggtgttcag cagcgagcag gagcgcaaat ggctcgaagc cctgctccat ccactgattg
     4861 ccgaagagat tcagtcaggt ctcgccaaca gccgctcgcc ctacacggtg ctggtttcgc
     4921 cgctgctgct cgaaaccacc cagcacagct acgtcgaccg catcctggtg gtcgacgtgc
     4981 cggtcgagct gcaactgcgc cgcaccatgg cgcgcgacaa caacagcgag agtcaggtgc
     5041 gcgccatcat cgcggcgcag atggcacgcg acacccgccg cagccgggcc gatgacctga
     5101 tcagcaacga tggcagcctc gaacagctcc agcagcaggt cgatgcgctg caccagcgct
     5161 atctggcact ggcggcagcc aaggccgcag gagcacccgc ttgaaccgat cgagcgccga
     5221 cgcgccgacc cgcatccacc cctgcccggt ctgccgcaag ccggtcgcct ggaatgccag
     5281 ctcgccgcaa cgccccttct gctcacgccg ctgccagctg gtcgacctcg gcacctgggc
     5341 cagcgagggc taccagattc cagccgaacc ttcgagtgca ttggagcagg caccgctcga
     5401 agacgaagag cagccgtagc cgaaccgcca ctcagccacg ccacaacgcc cggatcgccg
     5461 ccaccccctg cccgccgcgc tgcaggacgt ttggcagatc agcctcgcca accccgccca
     5521 acgcgtagac cggcaatgcc gccgcgctga cccactgctc gaaccgcgcc cagccgatcg
     5581 gctgcgccgc ggggtgtgag gcggtcggct gcaccggcgc cagcgtggca tagtcggcgc
     5641 cgatggccac cgcctgggcc agttcagcgg gtgagtggca ggaggcccca agcagcagcg
     5701 tggccggcag cggccgctcg cgcagtgcca gcagtttccg gctcggcagg tgcagcccat
     5761 ccgcactcac ctgctggagc agttcaggtt caccatgcag cagcagtttg gcacccaccg
     5821 cgcgacagcg cgccagactc agcttcgcca gtcgcaggta atcggactgc ttgacctccg
     5881 gcagccgcag ttgcagcagg cgaatgccgc gcgtcagggc ggcctcgatg ccggcgagca
     5941 gttcggtctc ggtcagccct gaaggtgtga tcagcaaccg ttgcggcagc agcaggctct
     6001 gcaccagcgg ccggttggcg gacagcagcg gcaggccgag cagctcttca gggcgacacc
     6061 agcgcagccc ctgcccttcg cgcggcgcgg gttcgccgct gaaggagtcg atggcgaaca
     6121 gctggatgcg cacccgcttg gtggggtaac ggtagggaag ttcgatcagc cgccgccccg
     6181 gagtggcgtc gatgccgagc tcttcgtgca gttcacgcct gagcgccgcc tcggcactct
     6241 cgccggcttc gatcttgcct ccggggaact cccagagccc ggcgaactcc ttgccggccg
     6301 gacggcagca ggccagtacc cggccactgc ggtcaaagag ggcggcggcg acgacctcga
     6361 ccgtcatcgc tggcctgcct caggtgcggt actcggcatt gatgcgcaca tagtcgtgcg
     6421 acaggtcggt ggtccagatc tgctgcgcca cgcgtccacg acccagttcg atgcgcaccc
     6481 ggatctcggc ccgatccatc accgcctggc cgcgctcttc acggtagtcg ggatcgcgtc
     6541 caccggcgct gacgatgcgc acttcatcca gatcgatgtt gatcagcccg acatcgagcc
     6601 cctcgacacc ggcacggccg acggcggcca gaatgcggcc ccagttggga tcactggcaa
     6661 agaaggcggt cttcaccagc ggcgagtggg ccaccgtgta ggcgacctgc aacgcctcgg
     6721 cctcactgcg cgcctgctcg acctcgatgg tgatgaactt ggtcgcccct tcgccatcgc
     6781 ggacgatcgc ctgcgccagt tcgcggcaga cctcggtgac cgcgtcgatg aactgccgcc
     6841 ccagcgggct gtcgaggtcg gtcagggcag tcgcgccact gcggccggtg gcggccagca
     6901 cacaggcgtc gttggtcgag gtgtcgccat cgatggtgat gcggttgaag gaggcatcga
     6961 ccgcctggcg cagcacggtg tcgagcagtg gcctcgacac ttcgaggtcg gtggcgagaa
     7021 aggccagcat cgtcgccatg tcggggcgga tcatgccggc ccctttggcg atgccggcca
     7081 cccggaccac tttgtcaccc tgcatgaatt cgcgctggct gcccttgggc aaggtgtcgg
     7141 tggtcatgat tgcccgcgct gccgcctccc agccatcggc agcgagtgcc gttaccagcc
     7201 tcggcagcgc cgtctcgaag cgcgccaccg gcagcggctc gccgatcacc ccggtcgaaa
     7261 acggcaacac ctcttcaggc cggcaaccgg tctcggcggc cagcagttcg cagcagcgcc
     7321 gtgcggccgc catgccgccc ggtccggtgc cggcattggc gttgccgctg ttgatcagca
     7381 aatagcgcgg cggtgccagc gccagatgcg tctcggccag cagcaccggc gccgcacgaa
     7441 aggcattgcg ggtgaagagc gcgctgacca cactgccggc atcgaaggca aagagggtga
     7501 ggtcggcgcc gccacggcgc ttgatgccgg cctcggcagt cgccagacgc actccggcaa
     7561 ccggctgaat cgacggccaa ggcagcggaa gcacggccat gctgttctat ctcctgatgg
     7621 atgagcggac gactaatcga gcttgccgtg gcactgcttg tatttcttgc cggagccaca
     7681 ggggcagggg gcgttgcggc cgaccttttc accgacacgc acatagggtt gcgcgccggc
     7741 tgcttcggcc gcttcctctt cggggggctg cagcgccgac gcctgatcgt gctgatagcg
     7801 cagcgcctgc cgcgcctgct cctcgcggcg acgccgctcg atctcctcga tgtcttcctg
     7861 cacgatttcg atgcgcgaca gcgtcttcac cacctcgtgc ctgaggttgc tcaacagctg
     7921 ctggaacagc tcgaacgcct cgcgtttgta ctcctgcttc gggttgcgct gtgcataggc
     7981 gcgcaggtga atgccctgcc gcaggtggtc catggtggcc agatgctctt tccacaggtt
     8041 gtcgagaatc tgcaacatca cctgtttttc gagcagccgc atctgcgtgc cgagaagctg
     8101 ccccttgcgc tcgtaggcct cgatcagtgc ctgcaggatg cgctcgcgca gcgtcgcctc
     8161 gtgcaggtcg ctctcctggt ccagccactg cgccagcggc agcgaaagcg ccagatcggc
     8221 actgaggtgc tgctccagac cggcaatgtt ccactgctct tcgaggctct gcggtggaat
     8281 gaattcgtcg atcacctcat tgaccaccac cttgcggaaa tcgacgatgg tgtcgctgat
     8341 gtcgtccgac tcaagcagcg cacggcgctg ccgatagacg atctgccgct gctcgttggc
     8401 cacgtcgtca tattcgagca gctgcttgcg gatgtcgaag ttgcgcccct cgaccttgcg
     8461 ctgcgccttc tcgatcgcgt tgctgaccat gcgatgctcg atcgcctcgc cctgctccat
     8521 gccgagccgc tgcatgaact ggcgcatccg ctccgaggcg aagatgcgca tcaggttgtc
     8581 ttcgagcgag agatagaagc gggaagagcc ggggtcgccc tgccggccgg accggccgcg
     8641 caactggttg tcgatgcgtc gcgactcatg ccgctcggtg ccgatgatgt gcagtccacc
     8701 actggccagc acctgctcat ggcgcagctg ccaggcctgg cgggccgcct cgcgctgctc
     8761 gggggtcacg ctgcccagtt gcgtcagctc gacctcgaaa ttgccaccca gcacgatgtc
     8821 ggtgccgcgg ccggccatgt tggtggcaat ggtgaccgcc ccgggttgcc cggcctgggc
     8881 gacgatctgc gcctcctgct cgtggtactt ggcattgagc accttgtgct cgatcttgcg
     8941 cttcttcaat gcgctggaga gcagctcgga actctcgatc gaggcggtac cgaccagcac
     9001 cggcctgccc tcggcacgac aggattcgat gtctgcgatg atggcgtcgt acttctcttc
     9061 cagcgtcagg tagaccaggt cgttgtggtc gatgcgcagc atcggcttgt tggtcgggat
     9121 caccaccaca tcgaggttgt agatctcctt cagctcaaag gcttcggtgt cggccgtgcc
     9181 ggtcatgcca gagagcttgt ggtagagccg gaagtagttc tggaaggtgg tcgaggccag
     9241 cgtctggctc tcggcctgca ccgccacccc ttcacgcgcc tcgatcgcct gatgcagccc
     9301 ttcggagagg cgccggcccg gcatcgtgcg gccggtgtgc tcatcgatca gaatgacctg
     9361 accattctgc acgatgtatt cgacatcacg atggaacagc acatgggcgc gcagtgccgt
     9421 gtgcacatgg tgcagcagcc cgagattggc cgccgaatag aggctctcgc cctcacccag
     9481 cagaccggct tcggtcaaca actgctcgac atgctcatgt ccctcttcgg tcagttcgac
     9541 ctgccgcagc ttctcgtcga ccagatagtg acccgccacc gccggctgaa cctcgctcgc
     9601 ctcgaccgcc cgggtcagct tcggaatcag ccggttgatg cgccggtaga gctcggagct
     9661 gtcttcggcc tggccggaga tgatcagcgg tgtacgtgcc tcgtcgatca ggatcgagtc
     9721 gacctcgtcg acgatggcga aattgagcgc gcgctggacg cgctcctcga ccgtgaaggc
     9781 catgttgtcg cgcaggtagt cgaagccata ctcgttgttg gtgccgtagg tgatgtcggc
     9841 cgcataggct tcacgcttgc tggcctgatc ctgccgcgac aggatcacgc cgacgctcat
     9901 gccgagaaaa cggtaaaccg gcgccatcca gttggcatcg cgctgggcca gatagtcatt
     9961 gaccgtgacc agatgcaccc ccttgccggc gagggcgttc agataggccg gcagcgtggc
    10021 caccagggtc ttgccctcgc cggtgcgcat ctcggcgatg cgcccctcat gcagcgtgat
    10081 gccgcccatc aactgcacat cgaagtggcg catgcccaaa gcacgccgcg ccgcctcacg
    10141 caccaccgca aaggcttccg gcagcagttg gtcgagcgtc tctccggccg ccagccgctg
    10201 gcggaactcg gcggtcttgc ccgccaattg gctgtcatcg agaccgcgac aactctcctc
    10261 gaagccattg atgcgggaga caatcttgcc catccgcttc agttcacggt tgtttttggt
    10321 ccctacgagc cgtctgacaa aagcggtcaa catgcctgaa tccggtgagc gttgcggtgc
    10381 aaaaaagtgg gccattctac cgtgaaaagc cggcagactt aagcagaggc ccggcacccc
    10441 gcgccgcaaa cccttgccag agcgcggtgg ccagcgcatt cttgcgcctg attcgaaggt
    10501 ggttgtttcc tcggcccgat tgatgcccat gcccgatcca gcacactccc gagcgcagac
    10561 ccctgcccgc ttcacgccac cccggcactc cacgctgggc gatctctatc agcaggccga
    10621 gactctggcg gcactgcagc ggctgctcgc cccactgttt gagcagttcg gcatcgactg
    10681 tgacgtggcc ggctaccgtg agggtcggct gctgctgatc tgcgctgacg ccgcgcgcgc
    10741 cctgcgcgcg cgccacagcg gcgcccgatt gctgctcgaa ctgcgccggc ttgatcggtt
    10801 cagggcgttg caacggatcg aatggcgggt gcgtccagtc cagcagccag cgcgtgcgct
    10861 gccactgccg cagcgaccaa agccagcgtc ggctcgcctg ctgagcgagc tggcgcacaa
    10921 cacggccgac tcacgactgc gcagcgcgct ggagaagctg gctcagccgg ccaagagtgc
    10981 ggaccggggg tgaagggagc agggagaggg ggtgatcagg ccacggtcag cgggcgggag
    11041 taggagatcg gcgcctgttc ggcatcgtcg aaggtgacca gctcccacgc ctccttgtcg
    11101 gccagcagcg tgcgaatcag ccggttattg agctcatggc ccgatttgtg accgacgaaa
    11161 cggccaatca gcccatggcc aagcaggtag aggtcaccga tcgcatcgag gatcttgtgc
    11221 ttgacgaact catcctcgta gcgcaggcca tcctcattga ggatgcggta ttcatcgacc
    11281 accactgcat tgtcgacact gccacccagg gccagatcac gcgcacgcaa ctgctcgatg
    11341 tcgctcatga agccaaaggt gcgcgcccgg ctcacctcct tgacgaagga ggtgctggag
    11401 aagtcgacct cggcgctctg cgtccggttg cggaataccg gatggtcgaa atcgatggtg
    11461 aaggccacct tgaaaccggc aaaggggtag agcgcggcgc tcttgtcgcc atcgcgcacc
    11521 tcgaccttgc gcttgatgcg gatgaacttt ttcggccgcg cctgctcttc gatgcctgcc
    11581 gactgcaaca ggaagatgaa gggaccggcg ctgccatcca tgatcggcac ttcggcacag
    11641 ttgatgtcga tgtaggcgtt gtcgatgccg agccccgcca tcgccgacag caggtgttcg
    11701 accgtcgaga tgcgcacgcc atccttgacc agggtggtgg agagggtggt gtcaccgaca
    11761 tgctcggcgc gggccggaat cagcaccatc ggctcgaggt cggtgcgaca gaagatgact
    11821 ccggtgtcga ccggtgccgg acgcaaggtc agatagacct tctttccact gtgaagaccg
    11881 acgccggtgg cccggatgac gttcttcagc gtgcgttgtc tgatcattga gccgtctctc
    11941 tcctgctgtc tgcgcgggcc agttcgccac cctgtggcca ggcccgctct attgcgatgt
    12001 cgacaccgtg ctgtcggtca gatggactga ccgcacagcg attagttccc cctgccagcg
    12061 gccaaagagg cggcatggta gcaaaagtgt caccctgcct ccagcgcgac ctggacagcg
    12121 caaggctgtc aatggacccg agtgcgcccc cgcaagaacc tgtccacctc aaatgttgct
    12181 ggcgggaacc ggcgtcagtc ggcctggcga cgcaggaaag ccgggatgtc caggtagtcg
    12241 atctcctgcc gcgccagctc acgcagacca ggctgcggcc gctgatcacg ctgcggcttg
    12301 agcgcctggg atccggcagt cagcggacgc tgcggccgca ccggaggctg gagcacgctt
    12361 ttctcccgcg gctcttcacc acgctgggtg tcgatcacgg tcaaggtcgg ctgggtggcc
    12421 gcaccaccaa ggccggtggc cacgacggtc acccgcagct cgtcggtcat cagcggatcg
    12481 atcgccgtgc ccacgacgac ggtcgcgctc tccgaggcga actcggccac ggtctcaccg
    12541 accgcggtga actcgcccag cgacagactc gggccagcgg tgatgttgac cagaataccg
    12601 cgggcaccca ccaggttgat gtcatccagc agcgggctgt gcagggcgcg ctgggcggcc
    12661 tcgtaggcgc ggttggcacc gctggcgcgg ccgctgccca tcatcgcgat gcccatttct
    12721 gacatgacgg tgcgcacatc ggcgaagtcg acgttgatct ggcccggacg catgatcagg
    12781 tcggcgatgc cctgcaccgc gcccagcagc acgtcgttgg cggcattgaa ggcatcggca
    12841 agcgaaacgt cgcgccccaa caccagttgc agcttttcgt tggggatggt gatcagcgaa
    12901 tcgacatgct cggccagctc ctcgatgccg cgctcggcga cctgcatccg cttgcggccc
    12961 tcgaacgtga acggcttggt caccaccgcc acggtcagaa tgccgaggtc acgggcaatc
    13021 tgcgccacca ccggcgcgcc gccggtgccg gtgccgccgc ccatgccggc ggtgatgaac
    13081 accatgtcgg caccacccag cacctcggcg atgcgctcac ggtcctcgat tgccgcctgt
    13141 cggccgacat cgggcgaggc acccgcgccc aaccccttgg tgatgccgct gcccagttgc
    13201 agcagggttc tggcctgcaa ccccttgagc gcctgggcat cggtgttggc gcagatgaac
    13261 tccaccccct cgacgtcgtt gctgagcatg tggttgacgg cgttgccgcc accaccaccg
    13321 acacccacca ccttgatgat cgggctctgg gtcacattgt ctacgagttc aaacatggtc
    13381 gcgctcctgt tttggtgatc gtttcctgaa aaaccttctc tgttccggcc agcctcggct
    13441 ggccgcccgg actacaaatt gtgctggaac cactgcttca gccgactgag cagattgccc
    13501 accgccggtc cgcgactgcc accgccggcg ggccgctgct catgctgtct gaagccgtgc
    13561 tgcagcagcc cgacagccgt ggcatagacc ggattggcga tgatgtcggc caagccatgg
    13621 acattctgcg gtacaccgag ccgcaccggc atgtggaaaa tctcttcggc cagctcgacc
    13681 gccccctcca tcttcgccgt gccaccagtc agcacgatgc cggccgtcat catgctttcg
    13741 tagccgctgc gttgcagttc ggcccgcacc agcgtgaaga gttcgtcgta acgtggctcc
    13801 accacctcgg ccagcgcctg ccgcgagagc tcccgcgccg gccggtcacc gacactcggc
    13861 acccggatga tctcatcggc acgcgccagc tgggtgtggg cacaggcata cttgatcttg
    13921 atctcctcgg cgctggcgac cggcgtgcgc agtgccaccg caatgtcgtt ggtgacctga
    13981 tcgccggcga tcgggatgac gccggtgtgg cgaatcgcgc catcatggaa aatggcgatg
    14041 tcggtggtgc cgccaccgat gtcgaccaga cagacaccga gatcgcgctc gtcgtcggtc
    14101 agcacggcgt agctggaagc gagctgctcc aggatcatct cctcgatttc gaggttgcaa
    14161 cggcgcacgc acttttcgat gttctgcgcc gagttgaccg cgcaggtgac cacgtgaacc
    14221 ttggcctcca tccgcacgcc ggacatgccg agcggctcct tgatcccctc ctgatcgtcg
    14281 atccggtact cctgcggcag gatgtgcagc accttctggt cggcgggcac cggcaccgcc
    14341 tgggcggcat cgagcacacg gtcgacatcg ctctggttca ccgcccgctc acggatggcg
    14401 acgatgccat tggagttgag actgcggatg tggctgccgg caatgccgac atagaccgaa
    14461 tggatctggc aacccgccat cagctcggcc tcttcgatgg cgcgctgaat cgactgcacg
    14521 gtcgactcga tgttgaccac cacccctttt ttcaggccgc gcgagcgctg ggtgccgatg
    14581 ccgatgacct cgatcacgcc gttgtcgctg cgctcgccca ccatcgccac caccttggag
    14641 gtgccaatgt cgagcgccac tatcatgttc gtactattcg ccaccatgaa ctccaggcat
    14701 ccgcctccga tcgaggccaa ttttcattgt catcagttct gctcccgcca tctgaccgcg
    14761 gcagcacttg ggtatctcat gtcgatcgcg gccacccgtg acagcgccgg cttgaggctg
    14821 tggcgcagca ggctggccag atggtcgatc cgtcgctcga ggtcacgact gcccagctgc
    14881 accttgacct cgccgtcgag ctgcagccac cagccagcgg ccgggtcata tcccagtccg
    14941 ctgcacggca ctccgacccg ctccagttgc tgcctgatct gctgatagcg ggccagaatc
    15001 tccttgccga gatgggtggg tccggccagc ttcggcagat gctcgtaacc ctcgatcgat
    15061 ggcgggtaga agggttcgcc ggcatcgttg agcagtccgc gctcgcccca gcgcgccacc
    15121 ggctgctgct cgaccaccgt caccgcgatc gccgccggcc agatccgctt cacctcgact
    15181 gcacggatcc agggcaaccc cagcaacggc gcccgcaccg cttcgaggtc gaccgtgaaa
    15241 aagcgtcccc gcgccacctg ctcgagttgg cgctgcagat gctgctgctc cagccggttc
    15301 agcgtgccat gcaccgccac ctgctcgatc agcggattga gccgctcgat cgccctgggt
    15361 gctccccaga cgatgccgac caccgcggca accagaccca gccgcagcag cagccagacc
    15421 agggcccggc cgacgtcgac cagttgctgc cagccctgca gcaccactgc ctgcatccgc
    15481 acatgcagtg gtggctgcgc caccctgggc cggatgcgct gattgacctt gcgccccgac
    15541 aacggacgcg gttcgattct gccggtcgcc gccgacattc tgccagtcgc gctcgggacc
    15601 ggccggctgc gcgccgccgt cggagccgct gccgtgcggc gcgccggtgt gactgcgcgc
    15661 gccatgccct gatcgcgcct ggcagaccgc gccggtttgc gtggcacttc gcgccgctgc
    15721 agtgtggcca tcgaaggccg ccccgatttg gcgggcgcct tcttcagcgg ctgcgcatga
    15781 cgtccaagca cgctctgcat catcaccgct ccctctcgga ggtggccaga atgtcgatca
    15841 ccagtgcctc gaatgagagt ccggccgcct tggccgccat cggcaccagg ctgtggtcgg
    15901 tcaggccggg gaccgtgttg gcctccagca gccagaagtt gtccgccgca tcggccagca
    15961 ggtcgatccg cccccaaccc cggcaaccga gggcggcaaa ggccgccagc gccagcgact
    16021 gcaccttctg ctcatcggcc ggcgcgagcc cgcaggggca gagatagcgg gtgtcgttgg
    16081 cctgatactt ggcgacaaag tcatagaagg tgtgcgaggt ctcgacccgg atcaccggca
    16141 gcgcctctcc atgcagaatc gacacggtgt attcggcgcc ctcgatccag cgctcggcca
    16201 gcacctgctg gtcgtaacgc agtgccagcg tcaatgcctg cgcatactcc gccgcggagc
    16261 cgacccggct gatgccaatg ctcgacccct catggttggg cttgatcgcc accaccggac
    16321 cgacccggtc cagcacctct tcccacggcg tctgcgccgt gacctgcatg aaaggcggcg
    16381 tcggcagacc cagcccccgc cacatctgct tgctcatctg tttgtccatc gccagtgctg
    16441 aggccagcgc cccgctgccg gtatagggac gatcgagcag atcgagcagc gcctgcagcg
    16501 agccatcctc gcctccgcga ccatgcaggg cgatgaaggc gcggtcaaag cggctgtcga
    16561 ccaagcgctc gaccagtcgc tcatcggcat cgaccacctc gacctcgatc ccctgtgccc
    16621 gcagcgcatt gagtaccgcc gcaccgctgc gcagggagat ctcgcgctcg gccgagcggc
    16681 cgccatggag cagcagcacc ttgccgaacc gctcacgcag tgaaaccgaa gccgtgctca
    16741 tcgatactcc gcccagttca gtctgctggc cgcgatgcgg ttggccaggg caccgacatt
    16801 gcccgctccc tgcaccagca ggatgtcgcc acccttgagc agttgccgca acagctccgg
    16861 tacagtggcc tgatcctcga cgaagagcgg ctcgaccggg cctctctggc ggatgctgcg
    16921 acagagcgcc cggccgtcga tgccgggaat cgccggttcg cccgccgcat agacctcgag
    16981 cagcagcaac agatcgggcc gcgacagcac ctgcacgaaa tcttcgtaga ggtcacgggt
    17041 gcggctgtag cggtgcggct ggaacagcat caccagccgc cgctccggcc agccggcccg
    17101 gatcgcatcg atggtgacgg ccagctcgcg cggatggtgg ccatagtcgt cgatcaccat
    17161 cacctcgccg ccgctgtcga gcgcaaactc accacgcaac tgaaagcgcc gccccacgcc
    17221 ggcgaactca cccagcccct gctggatctg ttcgacatcg acaccctcgt cgaccgccac
    17281 cgccatggtg gccagcgcat tgagcacgtt gtgccggccc ggcatcttca gctcgatcgg
    17341 caggccggaa ccacccggcc agcgcacgtt gaagcgcacg gtgttgcgct gctggacgac
    17401 gtcatacgca tggaagtcgg cctcttcgct gaagccatag gtgagcaccg gccggccgat
    17461 ctgcggaatc agtgcgcgca gccgtggttc atccagacag accaccgcca ggccgtagaa
    17521 cggcaggttg tgcaggaatt cgaggtaggt gcgctccagc cggttgacgt cgccgccgta
    17581 ggtctccatg tggtcgagat cgatgttggt caccacggcg accagcggtt gcaggtgcag
    17641 gaacgaggcg tcactttcgt ccgcctcggc gaccaggtag cggctggcac ccagccgggc
    17701 attgctgccg gcgctgttga ggcgcccacc gatcacatag gtgggatcga gaccggcctt
    17761 ggccagcacg ctggcgatca ggctggtggt ggtggtcttg ccatgggtgc cagcgacggc
    17821 gatgccatgc cggaaccgca tcagctcggc cagcatctcg gcacgcgaca ccaccggcaa
    17881 ccgctgggcc cgcgccgcca gcagttcggg attgtccgag gtcacggcgc tggagtagac
    17941 caccacatcg gcaccgctga tccgacccgc ctcgtgaccg aacccaatgg tggcaccgag
    18001 ccgttcgagc cggcgggtgt tctcgccggc cgacagatcc gaaccggaga tgcgatagcc
    18061 ctgattcagc agcacttcgg cgatgccgca catgcccaca ccaccgatgc cgatgaagtg
    18121 gatctggcgg atgcggcgca tctccggaat gtcgatggcg acccggttga ccggttcagg
    18181 cagcttgacc atgggccacc tcctgacagc aatcggccat gcgctcggcc gcctggggcc
    18241 gggccagggt ctggattcgc atgcccagcg ccagcgcacg ggccggttcg cgcacgaacg
    18301 gctcgagccg ggctgccagc gtcgaggcat ccagcgtggc ctgcggcagc agttgtgcgc
    18361 caccgacctg ctcaaccacc cgaccattga cactctgatg gtcatccacc gcaaagggat
    18421 agggcaccag cagcccaccg acaccggcgc agcagagctc ggccagggtc agtgctccgg
    18481 cccggcagag caccagatcg gcccagccgt aggcggtggc cattgcgtcg atgaagggct
    18541 cgacccgcgc ctcgacaccg gcctgtcgat agcccgcccg acaggcctca tccttgccgg
    18601 ccccggcctg atgccagacc cgcgggcgca gctcggccgg cagcagcgcc agcgcctgcg
    18661 gcagcagctc gttgagcgcg gccgccccca ggctgcctcc cagaatcagc agatgcagcg
    18721 gttcgccatc ggcacggcgc cgctcgaacc gctgtcgtgg cgattccaat gcacagatct
    18781 ctgcccgcac cggattgccg acatgctcca ccccggcgcc gccgaaggtg gcaggaaaac
    18841 cttcgaaggt gcgccgcgcc agcggccgca gcagccgatt ggtcactcca gccaccgcat
    18901 tctgctcatg gatcagcagc ggacggcgca gcagccaggc cgccagaccc ccagggccgg
    18961 ccgcaaaacc gcccatgccg agcacacagt cgggagcgat gcggcggatg cagcgcacgg
    19021 cctgccacac tgcccgcacc agttgcagcg gcgcgccgag ccagcgccgc actccagcac
    19081 cacgcagacc gccgacatcg aggcagttga gcgaaaaacc ggccgccggc accagccgct
    19141 gctcgatgcc gtggcggctg ccgagccact cgatcctggc gccccggctt tgcaatacat
    19201 cggccaccgc cagcgccgga aagacatgcc ctccggtgcc accggccatg atcagtaccg
    19261 tgaggctgcg ctggctcatg ggctctcctc ctcgctccgg atgggcaggc cgctttcggt
    19321 ctccacccgc agcaacagcg ccagcatgaa gccggtcacc acgatgctgc tgccgccata
    19381 gctgatcaac ggcagcgtga gccccttggt cggcagcagg ccagtgttga cgccgaggtt
    19441 gatcagtacc tggatcgcca gcatcagcgt gatgccgtag gccagataac cgccgaacag
    19501 ccgcccttcg cactcggcgt tgcgggcaat caccagcccg cgccagatca gcatgacgaa
    19561 gagcgcaacc agcgccagac aacccagcag tcccagctcc tcggcgatca ccgcaaagac
    19621 gaaatcggtg tgcgcctcag gcagatagaa gaccttctgc acactgttgc caagacccag
    19681 tccgaaccac tctcctcgtc cgaaggcaat cagcgcctgc accaactgat agccactgcc
    19741 atactggtcg gcccagggat cgatgtaggc cttgatccgc tccagacggt agggttgcgc
    19801 caccagcagc aggctgccca gggcaatgct gccgaggatc agcgtcgaga agcgcaacac
    19861 cgccatgccg gcaagaaaga acagacccag caccgtcgac atcagcacca ccaccgcgcc
    19921 atagtcgggt tcgagaatca gcagggcgat caccagcccc agaatcagca tcgggttgaa
    19981 aaagccggtc cagcgctgct gcacctcgac ccggtgacgc accaggtagc cggacagata
    20041 gatcaggatg aacgccttgg cgatctccga ggtctgcacg ctgacactgc cgaaaccgag
    20101 ccagcgcgcg ctgccattga ccttgcggcc aatgcccggc accaacacca gcaccagcag
    20161 cacgatggcg accagcaaca ggcgcgcgcc atgccgctcc caccaggcca acggcacccg
    20221 tcgggtcaac agcagcacac cggtgccgat cagcagccac agaccatgcc gcttgctgtg
    20281 ataccaggca tcccccaggt tggtggcatc gatgtcgacc gaagccgacg tgaccatgac
    20341 ccagccgaac agtgccagcg ccagcatcgg cagcgccagc agcaggtcga cccgatcagc
    20401 cgccaccggc cgcttgagca ggttgcccca cagggtggtc atggccggtg gactcagtga
    20461 catgctgcca actcccgcac tgcgcgctgg aacgccgcac cgcgctcggc aaagccgctg
    20521 aattgatcga aactggcaca ggctggtgcc agcagcacgc aatcgccggt ctgcgccaga
    20581 tcacgcgcct gccggacggc ctcatcgatc ccttgcacct gcacactggg cgtgaccgga
    20641 tcgagcgcgg cagcgatttc gccaccatca acccccatca gcaccaccgc ccgcgcgtga
    20701 ttccgcaccg gctcgcgcag gccggaaaaa tcggcgccct tgccgacacc acccagaatc
    20761 agcaccagct tgccagcgag atcggctgcc agaccccgta tcgccgcgac ggtggccccg
    20821 acattggtcg ccttggaatc atcgacatag tcgacgccat cgatgcggtc gatccattgg
    20881 cagcggtgcg gcagaccacc gaactcgagc agtgcctcga ccatcgcagc gcgcggcagg
    20941 ccgattgcgc tgccggccgc cagcgcggcg agcatgttgc cgtggttgtg caggccgcgc
    21001 agcttgagcg catcggttgc cacccaaggc tcgtcccctt gggcgagata gcgcctgcca
    21061 ccctgctcga tcaggccgaa ctcctgcgca ctggccggcc gtcgcagccg gtaggaccag
    21121 cgccgcaccg tcgccccgtc catcctggtc tcggggtcgt cactgttcgt caccacttgg
    21181 cggacgccat tgaagatgcg ccgcttggcc gcgcggtaac tggccaggtc agcgtagcgg
    21241 tccatgtggt cttcgctgag gttgagcagc agcgccactt cggcatccag ctgctcgacc
    21301 agctccagtt gaaagctgga gagctccagc accaccacct cggctgcgcc ctgttccagc
    21361 agctccagcg ccggcgtgcc gaggttgccg cccaccacca cccgacagcc ggcacgccgt
    21421 gccatctcac cgatcagcgt ggtcaccgtg ctcttgccgt tggagccggt cacggccacc
    21481 agtgcgccct cggtctgcgc ccggaacaac gcgatgtcgc cggtgatgcg cgcgcccgcc
    21541 gctgcggcct ggcggatcgc cggctcgccg agcggtacgc ccgggctcac caccaactgc
    21601 tcgaaacggc tgaacagcgc tccatcgaaa ggccccaggt ggcaggccac gcccggctgt
    21661 tcagtgcgca gctgctccag cccgggcggc atggtgcggc tgtcggccac ggcgaaatgg
    21721 atgccctggc gcgccagaaa acgggccacc gacaggccag tcgcgcccag ccccaccacc
    21781 agggtctgca tcttttcagt ggtcgcgaca ctgtgcatgc gtcacctcag cttcagcgtg
    21841 gccagaccga tcagcaccag caccagggtg atgatccaga aacggacgat cacccgcggt
    21901 tcgggccagc ccttcagctc atagtggtgg tgcagcggcg ccatgcggaa gatgcgccgc
    21961 ttggtcagct tgtagtagcc gacctgcaga atgaccgaga ccgtctccat cacgaagacg
    22021 ccccccatga tgaacagcac gatctcctgg cgcacgatga ccgccacggt gcccagtgca
    22081 gcgcccagcg ccagcgcgcc gacatcgccc atgaagacct gcgccggata ggcgttgaac
    22141 cagagaaatc caagtccggc accgacgatc gccgcacaga acaccagcac ctcgccggcc
    22201 cccttgatgt gcggaatcag caggtagtcg gcaaaaccgg catggccggc gacataggcg
    22261 aacaggccca atgcaccgcc gaccatcacc gtcgacatga tcgccagccc atcgaggcca
    22321 tcggtcaggt tgacggcatt gctggtgcca acgatgacga agtagaccag cgggatgaac
    22381 agcaacccga ggttgatcga gacattcttg aagaagggca gcaacagctg ggtctgctcc
    22441 gcaatggtgg cattctggaa caggaacagc gcggcggcga tgccgatcag cgactgccag
    22501 aaatatttcc agcgcgccgg caggccgatc gagttcttct gcaccacctt gcggtagtca
    22561 tcgacccagc ccaccgcgcc aaacagcagg gtcacgccca gcagcagcag gatgtagcgg
    22621 ttgctcagat cggcccacag cagcgtgctg gtcagcatcg acagcagaat cagcaccccg
    22681 cccatcgtcg gcgtgccggc cttgctcaga tgggtggcgg gtccaagcgt gcggatgctc
    22741 tgcttgatct gcaatccggc cagacggtcg atcacccagg gaccggccag cagcgcgatc
    22801 gccagcgcgg tcagcacgct gagaatcgcc cgcagcgtga ggtagcggaa cacgccgaac
    22861 agcgactcat aaccggccag ctgacctgtc agccacagca acatcagcag acctcctgct
    22921 ggctcagcgc ctcgaccacc cgttcgagcc gggcactgcg cgaccccttg atcagcacca
    22981 cccagccctg ctgcaaccgt ggcttcagcc aggcaatcgc cgcatcgaca ttgccgcagg
    23041 ccacggcgcc ggcaccgaag gtttcggccg ccactgccgc cacctcaccg accgtcacca
    23101 gctgatcgac cccatgctgt ctggcatggc ggccgatgtc gcggtgcagc tccagcgtcg
    23161 tctcgcccag ttcggccatc tgtcccagca ccagaatgcg ctccccctcc tgcaggcaga
    23221 gcagatcgat cgccgccttc actgccgtcg ggctggcgtt gtaactgtca tcgatcacca
    23281 ctgcaccgga actgccctgc aaccgcatca cccggccggc ggttggcttc acccgcgaca
    23341 gaccgcgggc gatgctggtc agatcgatgc cgaacgccac ggccgccgcc gaagccgcca
    23401 gcgcattgga aaccatgtgg cgaccgtaga gcctgagcgc gacggcgatc tggcctaccg
    23461 gcgtgttcag ctcgaaacgg taccgcccat ccgcatccgc cacctggtcg gtcgcggtga
    23521 tctcggcatc gctgccgccc tgcaggctga agctgatgac gcgccgacct gccgcccgtt
    23581 cacgccaggc gtcgtagccg gcatcatccc ggttcaacac cgcaacgcca tcaggcggca
    23641 gggcatccag aatctcaccc ttggccgcca cgatcccggc gtgggaacca aaaccctcca
    23701 gatgggcctc gccagcattg gtgatcaccg ccaccttcgg cttggccaga gcggcggtgt
    23761 aggcgatctc gcccaggtgg ttggccccca gctccagcac ggcatagcgg tgctgcgctt
    23821 ccagttcgag cagcgtcagc ggcacgccga tctcattgtt gaggttgccg cgggtggcat
    23881 gggtcggcgc cacttcagcc agaatcgacg cggtcagctc cttggccgtg ctcttgccgc
    23941 aactgccggt gatcgccacc agctctccgg tgaagcggcc gcggttcaac tccgccagcc
    24001 gccccagcgc cagccggctg tcactcacct gacagtgcgg cagcggatag ggaatcggct
    24061 gctccaccag cgccgccacc gcgccgcgtg caatggcctg atcgatgaac tgatgtggat
    24121 cgaattgcgg tccgggcagc gccacgaaca gatcgcccgc caccagcttg cgggtgtcgg
    24181 tgcagaccga gccgaaacag acatccgcac cttgcagccg gcccgcgcac gctgcacagc
    24241 aatcactcag tttgaagctg ccgatcatgc gacccggtac ctttgcagcg cggcctcgac
    24301 gatcgcatgg tcgttgcagg gatggcgctg acctgccacc tcctggtaac gctcatgccc
    24361 cttgccagcc accagaatcc aatcctcggc ggccgcctgt ccgatgacct gctcgatggc
    24421 gcgcgaccgc tcatgctcaa ccagcacctc ggccgcaccg acaccagaga ggatctggtc
    24481 ggtgatggcg acgggcgact cactgcgcgg attgtcatcg gtcaccatca cccggtcggc
    24541 caggcggacg gcaatcgccc ccatcaacgg ccgcttgtcg cgatcacggt cgccaccgca
    24601 accgaacaga caccagagcg ctccgtcaca gtgctcgcgc agtgcttcga gcacgctctg
    24661 cagcgccgct ggcgtgtggg cataatcgat caccaccgtg ggcctgccgg caccgcccag
    24721 ccgctccatg cgcccctcga ccggccgcac cgcacggcat gccgccaaca gggcagaaaa
    24781 ctcaaaaccg cgacccgccg cgaccgccag cgctgccagc agattggcca ggctgaaacg
    24841 accgagcagc gtcgtcacca gttcgccaca tccccaaggg gtggtcagcc aggcgcgcaa
    24901 ctcaccctgg gcgatcgacc ggatttcggc atgc
//
LOCUS       DAORCQ010000029        24105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1194_length_24105_cov_5.829688, whole genome shotgun sequence.
ACCESSION   DAORCQ010000029 DAORCQ010000000
VERSION     DAORCQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 24105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24105
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_24105_cov_5.829688"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKI23_04740"
     CDS             complement(<1..874)
                     /locus_tag="PKI23_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNN36282.1"
                     /translation="MDKSYQPAQLEQQWYARWEAGDHFRPDPAQSGEPPYCIMIPPPN
                     VTGTLHMGHAFQDTLMDVLTRYHRMRGAPTLWQPGTDHAGIATQMVVERQLAAQGLSR
                     HDLGREAFLERVWQWKAESGNTISRQLRRMGASADWSRERFTMDAGLSAAVTEVFVRL
                     YEEGLIYRGQRLVNWDPVLHTAISDLEVVSEEEEGSLWHIRYPIADAAGQPTAEWLIV
                     ATTRPETLLGDTAVAVHPEDERYRHLIGQQVLLPLTGRLIPIIADEYVDPAFGTGCVK
                     ITPAHDFNDHAVGSR"
     gene            1112..1510
                     /gene="mscL"
                     /locus_tag="PKI23_04745"
     CDS             1112..1510
                     /gene="mscL"
                     /locus_tag="PKI23_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNN36283.1"
                     /translation="MSLIKEFKEFAIRGNVIDMAVGIIIGAAFGKIVSSLVGDLIMPP
                     IGLLLGGVDFSDLAITLKAAAEGAPAVVISYGKFIQTVIDFIIIAAAVFVGVKAINQL
                     QQQKQAAPTAPSNEEKLLSEIRDLLKSQSR"
     gene            1686..3500
                     /locus_tag="PKI23_04750"
     CDS             1686..3500
                     /locus_tag="PKI23_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNN36284.1"
                     /translation="MSTLRNPETLPDRHLLAEIGNQLEQLRQAVIGDAARLQSASRGD
                     DDTPSLRNFAHYLALRSHDLRPLQALLGQCALSSLGRSEAHVMATLDCLLALIAAVLG
                     RSEPIEPAAPVTFDMGRQLLSANRIRLFGPLPSDRPSHIMVTMPRAVASDPGLAMQLV
                     AAGMDCARINCAHDDAGIWEAMVRNLRAAALAAGRECRILMDLAGPKLRTGPVALADC
                     MRLKGVVMLVSGVPTEAGLLGFPAELIAALAPGDVITLRDRRAKKRELLIEGREPDGR
                     CRALCERAAQIDAQTEFCITLGGQRRQARAAMQMPPVEIRLFEGDSLLLDRAARPGVA
                     AQPDGTPARIGCGEPAVLDQLTAGMAVWIDDGKIGALVERIDAEGALLRITAAGPKGR
                     RLRADQGLNFPDSALRLPALSDKDRRDLDFAIHHADLIGLSFAQSRADLEALRTALAE
                     RGAPLLPIIAKIETAAGVGNLPEMLLAMVDRHPFGVMIARGDLMVEIGGERMAELQEE
                     MLWLCEAAHVPVIWATQVLESLTHDGSLSRPELTDAAASARADCVMLNKGPHLLRALS
                     VLVDIHTRMAGHQQKKNPQLRALSLASRLLENGDGPAA"
     gene            complement(3507..4241)
                     /gene="rlmB"
                     /locus_tag="PKI23_04755"
     CDS             complement(3507..4241)
                     /gene="rlmB"
                     /locus_tag="PKI23_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNN36285.1"
                     /translation="MSQTVYGVHAVTALLHSDPTRISRVLLQQGREEKLQALIKQLRA
                     QGIAIEQRSRRELDQLAPGVHQGVVALVETSVEAGENELFALLDGLQRPAFLLVLDGI
                     TDPHNLGACLRTANAAGADGIVLPKDRSAPLNAVARKAASGAAELTPCFRVTNLARTL
                     GALKAQGLWLVAATADGPGTLFQADFKGPIALVMGAEGHGLRRLTREQCDFEVAIPMK
                     GAVASLNVSVASALLLYEVMRQRDSG"
     gene            complement(4275..6608)
                     /gene="rnr"
                     /locus_tag="PKI23_04760"
     CDS             complement(4275..6608)
                     /gene="rnr"
                     /locus_tag="PKI23_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNN36286.1"
                     /translation="MAGHPGNDDPFLAREQQKYSHPIVSREFILKWLSERKGPASHAR
                     LCEELGIASADDVEALRRRLIAMERDGQLIRNRRDAYLPVDKMDSIAARVQGHKDGFG
                     FAVPGDGSADLYLGAREMRKVFDGDLVLVRKVGEDRRGRPEGVIVEVVEHRTWALVGR
                     YFEERGVAFVRAENRRIQHEILIPPGEAGHARHGNYVMIELIQQPGPRQLPQGRVVEL
                     LGEYLAPGLEIEIALRSHQLPHRWSEEVEREAARLGDSIAADELTRRVDLRSLPFVTI
                     DGEDARDFDDAVLCERQAHGGFLLRVAIADVAHYVTPGSALDAAAHERGTSVYFPSQV
                     VPMLPEALSNGLCSLKPRVDRLVLVCEMVIDRKGQIDDFCFYEGIIHSHARLTYSEVA
                     ELLAAPAEAPLRQQSPHRPLLPQLENLHALYRVLHQARRARGAIDFDTLETRILFDAE
                     RKIERIVPVVRNDAHRLIEEAMLAANVCAARLLLHHKVPALFRVHEVPDETKLTRLHA
                     YLAELGLSLSGGKKPSPKQMQKLLDTIAARPDAHHIQMVMLRSMRQALYQPENLGHFG
                     LGYEAYTHFTSPIRRYPDLLVHRAIRATLRSRKPAQHLRRVKGAGVLRKQQVYPYDPQ
                     GLALIGQHCSQTERRADAATRDVVDWLKCEYLRAHVGSEFDGLITSVVAFGLFVELRD
                     IFVEGLVHITALPNDYYHFEESKHRLVGERGRAVYRLGDPLRVKVVRVDLDERTIDFE
                     PVLQRPAATPGSGKGGKKNKATQDGTGGGKRRRRKSG"
     gene            6819..7880
                     /locus_tag="PKI23_04765"
     CDS             6819..7880
                     /locus_tag="PKI23_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNN36287.1"
                     /translation="MKLPTHARIAAAALLCIGLPQLAQASAVYGTLSNFDVYNDSTTP
                     YHGFEIELEDFSLSQLAGYNGNYYTYPNWHYGSGQVSQSGTNVLIRYSKAGGQTAPFS
                     GNVVATNGHACITIAGCEHFGVSLNGAPTATRYFWLDENGNRSTQVNLLAPVMTVVQP
                     AVPAQNGNPPQPAIVRAEVEAPEVIKANGRSDAIWVQVFKREVDNDQEGEVELDDLMA
                     DNGGVVADEGDGVEIEWKLFQRKPGDDSANLLDSGEQEVGDGMEQVLRTYRFYKFAGT
                     YDASHEATCTEVGNCEDLAEAGEHMDYVGALIGQQMVALNLDGALPPAPVPLPASVWL
                     LGSGLFGLTALARRHSRGV"
     gene            8097..8173
                     /locus_tag="PKI23_04770"
     tRNA            8097..8173
                     /locus_tag="PKI23_04770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8131..8133,aa:Arg,seq:acg)
     gene            complement(8216..10387)
                     /gene="uvrD"
                     /locus_tag="PKI23_04775"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(8216..10387)
                     /gene="uvrD"
                     /locus_tag="PKI23_04775"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HNN36288.1"
                     /translation="MNSSTPIDQLNPAQRDAVRASATHQLILAGAGSGKTQVLVHRIA
                     WLIQRHQVPPHAILAVTFTNKAAREMRERIETLLGHDIRQMWVGTFHGLAHRLLRLHW
                     HDAGLAEDFQILDSDDQQRLIKRTLRELGLNEEQWPARQAQWFINAQKDEGIRPNRIE
                     PQGDRQLATLVEIYQAYEAACQRANVIDFAELLLRAHELWLNHPELLHHYQNRFRHLL
                     VDEFQDTNSIQYAWIRLLAGGGARVTAVGDDDQSIYGWRGARIENIHRFSRDFADVQT
                     VRLEQNYRSTATILDAANAVIARNRERLGKALWTDGSPGEPIALYTAYNEEDEARFVV
                     GQIRQQLAQGLACREIAILYRSNAQSRLLEEALLRAGIAYRIHGGQRFYERLEIKNAL
                     AYLRLMLNRHDDTSLERVLTAPPRGIGGKTLELLRSEARGQQWSLWRAMQQMVTARAL
                     PARALTALAAFTQNIDELDRATRDLALSQRVEQVIQRSGLIEFHASERGEKGRGRVEN
                     LQELVSASGQFSELLRSEDETLLATAAHDELALLLDSVALDAGAEQGGEVLDAVQLMT
                     LHSAKGLEFPVVFIVGIEEGLFPHQMSLEEPGRLEEERRLCYVGLTRAMHKLYLSHTE
                     SRRLHGTLCHNRLSRFVREIPPHLLQQASSGGSVGHGQIRTDVGEEQGLQLGARVHHG
                     RFGDGTVIDCEGSGNQTRVRVNFDREGEKWLVLAYARLEPI"
     gene            10654..11070
                     /locus_tag="PKI23_04780"
     CDS             10654..11070
                     /locus_tag="PKI23_04780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNN36289.1"
                     /translation="MSASTQLLAHNWSFGIYLFAIAGVCALMLLLSWLLGGRAWGRAK
                     SEAFESGVVSVGGARLRFSAKFYLVAMFFVIFDVEALFLYAWAISLRESGWAGFVEAT
                     IFIFVLLVGLVYLWRIGALDWAPQGRGQRAQPRGEK"
     gene            11073..11741
                     /locus_tag="PKI23_04785"
     CDS             11073..11741
                     /locus_tag="PKI23_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNN36290.1"
                     /translation="MDYTLTRVDEEQQRYPLEQRIPVADPLEQHVHKSIYLGKLEQVL
                     HNAVNWGRKHSLWPYNFGLSCCYVEMATSFTAPHDVARFGAEVIRASPRQADFMVIAG
                     TCFIKMAPVVQRLYEQMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYIPGC
                     PPRPEAFLQGLMLLQESIGRERRPLSWVVGEQGVYRAEAPSQRELRREERMSMTTLRT
                     PDQV"
     gene            11814..13565
                     /gene="nuoC"
                     /locus_tag="PKI23_04790"
     CDS             11814..13565
                     /gene="nuoC"
                     /locus_tag="PKI23_04790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HNN36291.1"
                     /translation="MASGDQHQVVRALRARFADVELVFQPTLDELPTLWVARERLLDL
                     LRHLRQLPQPYVMLYDLSAVDERLRTQRPGLPKADFSVFYHLLSIERNSDLRIKVALS
                     QDDLRLPSTVSIWPNANWYEREVWDLFGIEFDGHPHLERIMLPRTWSGHPLRKDYPAR
                     ATEFDPFMLDAAKQDLEQESLRFVPEEWGMKRHSSDADFMFLNLGPNHPSAHGAFRIV
                     LQLDGEEILDCVPDIGYHHRGAEKMAERQSWHSYIPYTDRIDYLGGVMNNLPYVLAVE
                     KLAGIEVPDRVKTIRVMMAEFFRITSHLLFLGTYIQDVGGMTPIFFMFTDRMKAYDVI
                     EAITGGRMHPAWYRIGGVAHDLPTGWERLVREFVNWLPKRLDEYEKAAMRNGILRART
                     IGVAQYDTAAALAWGVTGAGLRATGLGYDLRKARPYSGYENFEFEVPVAANGDAYDRA
                     MVRLEEMRQSLRIIKQCLEQMPAGPYKADHPLTCPPPRERTLQDIETLITHFLSVSWG
                     PVMPAGESSQLIEATKGINSYYLTSDGGTMSYRTRIRTPSFPHLQQIPSVIRGSMIPD
                     LIAYLASIDFVMADVDR"
     gene            13552..14085
                     /gene="nuoE"
                     /locus_tag="PKI23_04795"
     CDS             13552..14085
                     /gene="nuoE"
                     /locus_tag="PKI23_04795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNN36292.1"
                     /translation="MSTDNPNSTSAQRLTSERHPGGFELSAAERAAIEHEMSHYEDPR
                     AASIGALKIVQQARGWVPDGAIAAIGNLIGIPASDVEGVATFYNLIFRQPVGRHVIKL
                     CDSIACFLTGYDELKAALTTHLGIDFGQTTADGRFTLLPICCLGNCDKGPTLMIDDDL
                     HGPVAPGQVAALLEGYP"
     gene            14094..15389
                     /gene="nuoF"
                     /locus_tag="PKI23_04800"
     CDS             14094..15389
                     /gene="nuoF"
                     /locus_tag="PKI23_04800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNN36293.1"
                     /translation="MARSAETHPLTYRLESGEPVVDLKAYEALAGYAALRKAVPAMTP
                     AEVAQVVKDANLRGRGGAGFPAGIKWGLVPMGADAPAQKYLVCNADEMEPGTFKDRLL
                     MEKLPHQLIEGMIVAAYAIQASRGYIFLRGEYVLAAERLNRAIAQAIEAGYLGQNILG
                     SGWSFDLFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPQIAGLWGKPTVVNNCE
                     TLNNVAPIVLHGADWFKGLSQGKSPDGGTKLYGASGRVKRTGVWELPIGTTAREILEQ
                     HAGGMQDGLALKAWLPGGASTDFLLPSHLDLAMDFDTIMKAGSRMGTGLIMAIDDRQS
                     MVSVVRNLEIFFARESCGWCSPCRDGLPWTVKILSALERGEGQAGEIEILEQIARQLG
                     PGKTFCAHAPGAVEPLQSAIKFFREEFERGIAAPEAERA"
     gene            15438..18179
                     /gene="nuoG"
                     /locus_tag="PKI23_04805"
     CDS             15438..18179
                     /gene="nuoG"
                     /locus_tag="PKI23_04805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004417260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNN36294.1"
                     /translation="MATIHVDGKEYEVDGADNLLSACLSLGLNVPYFCWHPALGSVGA
                     CRQCAVKQYANADDSRGRLVMSCMTPAADNSYISIDDEEARQFRASVIEWLMINHPHD
                     CPVCEEGGNCHLQDMTVMTGHDRRRYRFTKRTHPNQELGPFIGHEMNRCIACYRCVRY
                     YRDYAEGEDFGVYGAHDNLYFGRLEEGVLENEFSGNLVEVCPTGVFTDKTHSARYNRK
                     WDMQFAPSVCQHCAVGCNISPGERYGELRRVENRYNGAVNHYFLCDRGRFGHGHVNSA
                     ERPRTPLLRQGDALVSATIDTALTTAGEALRHVRRIIGIGSPRASVEANFALRELVGA
                     DNFSVGMADQEWRVVRKILDVLGSTTAEIPTLREMEERDAVFILGEDVTQTAPRIALA
                     LRQATKGKARALAAAKKAFVWQDEAIRNIGQQARHPLIVTGVIESRLDDAASELHRDT
                     PEALAQLGFAVAHAIDAEAPAVALAPEQLERAQRIAATLLAAERPLLVSGSSLGCEAL
                     IDATANIATALRRQGRAAAITLVVPEVNTLGVAMLGGVSVDVALAELQQGAADGLIVL
                     ENDLYRRVDAATVDAALDSARMVVTVDHQLTATVARSQVTLPAASFAEGDGTVVSQEG
                     RAQRFFQVYDPGYYNLASQLKESWRWLEGLHSVIGGELPGWDLLDQITAACAAKVPAL
                     RRISEAAPDANFRVAGRKIARQPLRYSGRTAMRANLSVHEPRASQDGDSALTFSMEGY
                     GGPAEPGALIPFAWAPGWNSPQSWTRFQDEVGGHLRGGDGGVRLFEASTPQVGEYFRE
                     QPVLPISAEGRFRLLPLYHIFGSEELSIRAPAVASRAPQPYLAVAQADASRLGIAEAD
                     QVEVTIGTRLLRLPARVSATLVAGTVGLPAGLTGLPPLPALGWSELKRLETGKVATA"
     gene            18176..19168
                     /gene="nuoH"
                     /locus_tag="PKI23_04810"
     CDS             18176..19168
                     /gene="nuoH"
                     /locus_tag="PKI23_04810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNN36295.1"
                     /translation="MNWLTPDLLTITLAVVKAVVILLVVVLFAAVLSWIERRLLALWQ
                     DRYGPNRVGPFGAFQIVADMIKMFFKEDWTPHFVDRLTFVLAPVIAMSALLIAFAIVP
                     ISPDWGVADLEIGILLFFALAGLSVYAVMFAGWSSNSKYSLLGGLRSAAQTVSYEVFM
                     GLSLMGVVAQAGSFNMREIVAAQEQLWFIVPQFFGFITFALAGVAVAHRSPFDLPEAE
                     QELAAGYHTEYSGLKWGMFFVGEYVGVVLVSALLVTLFFGGWHGPFLPPALWFILKTL
                     FFVMLFILIRGALPRPRYDQIMAVGWKVCLPLTLINLLVTAAVVLSQSGVNQAG"
     gene            19179..19718
                     /gene="nuoI"
                     /locus_tag="PKI23_04815"
     CDS             19179..19718
                     /gene="nuoI"
                     /locus_tag="PKI23_04815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNN36296.1"
                     /translation="MLIKLITGFGSQLRSMWMVFAHAFRKRDTIQYPEQKVYLPPRYR
                     GRIVLTRDPDGEERCVSCNLCAVACPVGCIALQKTETEDGRWYPEFFRINFSRCIFCG
                     MCEEACPTTAIQLIPDFELAEYRRQDLVYEKEDLLIAGCGKNPDYNFYRQAGMAIAGK
                     PKGAANNEAEPISVKTLLP"
     gene            19757..20263
                     /gene="nuoJ"
                     /locus_tag="PKI23_04820"
     CDS             19757..20263
                     /gene="nuoJ"
                     /locus_tag="PKI23_04820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNN36297.1"
                     /translation="MELAFYLSAAVALLATLGVIIARNPVHALLYLVVSLLAVAMIFF
                     AIGAPFAGALEVIVYAGAIMVLFVFVVMMLNLGPSVTEQERLWTQPRLWIGPSLLSAL
                     LLAGLLRVILADASQLAGLESVGAKQVGIALFGPYLLAVELASLLLLGALVAAYHLGH
                     IESDKDKK"
     gene            20336..20644
                     /gene="nuoK"
                     /locus_tag="PKI23_04825"
     CDS             20336..20644
                     /gene="nuoK"
                     /locus_tag="PKI23_04825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNN36298.1"
                     /translation="MTGIPMAHGLALSAMLFTLGLAGLMVRRNLLFMLMCIEVMMNAA
                     ALAFVMAGSHWLQPDGQIMFILILTLAAAEASIGLAVLIQLYHRHHTLDIDVASEMHG
                     "
     gene            20641..22488
                     /gene="nuoL"
                     /locus_tag="PKI23_04830"
     CDS             20641..22488
                     /gene="nuoL"
                     /locus_tag="PKI23_04830"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNN36299.1"
                     /translation="MNLLFLTILFPLLGYLLLAFSRGRWSERQVALVGVGSVAFAAGV
                     AGWVGVEFLQQPRGVIGYTQLLWNWIEAGNFKSDFTLRLDGLSLTMLGVVSGVGFLIH
                     LFASWYMRGEEGYSRFFAYMNLFVASMLCLVLGDNLLLLYLGWEGVGLCSYLLIGFYY
                     KESANGAAAQKAFIVTRVGDVFMAIALFALYLQFGTLDIQTLLAQAPQVWQSGGAAPT
                     LVALLLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFL
                     LAPQVLELVALVGTLTLLLAGCAALVQTDIKRILAYSTMSQIGYMFLALGVGAWNAAI
                     FHLMTHAFFKALLFLSAGAVIIACHHEQNIFKMGGLRKKLPLVYAAFLVGGSALAALP
                     LVTAGFYSKDAILWGALASGHDLLVVAGLVGAFLTSLYTFRLIFIVFHGESQCDAHAG
                     HGLAHGLPLLFLLLLSTLVGAWITPPLTAVLPTDPGAAGGEAKHLLEWISGGVAVAGI
                     AMAALLFLGKRQFVDRVATAGPGRLIGQLWLNAWGFDWLYDRLFVKPYVWLAHLHRND
                     PVDQAILVIPFLARTGHDALSRVQNGRLRWYVASIGLGAVLVVALLLLG"
     gene            22510..24042
                     /gene="nuoM"
                     /locus_tag="PKI23_04835"
     CDS             22510..24042
                     /gene="nuoM"
                     /locus_tag="PKI23_04835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009877657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNN36300.1"
                     /translation="MILVWLFLIPTLGGLLAWGGERYGSGIPRWVSLLSITSVFVLSL
                     LLWQQGDYSLGQGVSAQPQWQLEFQAPWISRFGISFHFALDGLSLVMVMLTAFIGILS
                     VVCSWREINRHVGFFHFNLLLLLSGVIGVFLAIDLFLFFFFWEMMLIPMYFLISLWGH
                     KGAKGSSRIQAAIKFFIFTQAGGLLMLLAILGLVLTHYSVSGELSFDYEVLRHAILAP
                     ELEYALMLGFFMAFAVKLPIVPLHSWLPDAHSQAPTAGSVDLAGLLLKTAAYGLLRFV
                     LPMFPNASHEFAPIAIGLALVGIIYGAVLAFSQHDIKRMVAYTSISHMGFVLIGIYAG
                     STVSLQGVVVQMVAHGLSAAALFILCGMLYERLHTRDLRQMGGLWSRLPLLPAVALFF
                     AAASLGIPGTGNFIGEFLILLGSYQAAPYAVMAATGGLVLASIYSLRLVQRAFFGPAQ
                     SSEPIEAATGRETVMLLGLVVLTLWIGFYPQPLLNTSAAAIGQVQQIYAAAIQAAEAA
                     AP"
BASE COUNT         3850 a         7504 c         8056 g         4695 t
ORIGIN      
        1 gccggctgcc gacggcgtgg tcgttgaagt cgtgcgccgg ggtgatcttg acgcaaccgg
       61 tgccgaaggc cgggtcgacg tactcatcgg cgatgatcgg gatcagccgg ccggtcagcg
      121 gcagcagcac ctgctggccg atcaggtggc ggtagcgttc atcttctgga tgcaccgcga
      181 cggcggtgtc gccgagcagg gtctccgggc gggtggtggc aacgatcagc cattccgccg
      241 tcggctggcc cgcggcgtcg gcgatcgggt agcggatgtg ccagagcgag ccctcttcct
      301 cctcgctcac cacctccaga tcggagatgg cggtgtgcag caccggatcc cagttcacca
      361 gtcgctggcc gcgatagatc agcccctctt cgtacaggcg cacgaacacc tcggtcaccg
      421 ccgccgacaa gcccgcgtcc atggtgaagc gctcgcgcga ccaatcggcc gaggcgccca
      481 tgcggcgcaa ctgccgcgag atggtgttgc ccgactcggc tttccactgc cagacccgct
      541 ccagaaaagc ctcacggccg aggtcgtggc gcgacaggcc ctgggcggcg agctgacgtt
      601 cgaccaccat ttgcgtcgcg atgccggcgt ggtcggtgcc cggctgccac agtgtcggcg
      661 cgccgcgcat gcggtggtaa cgcgtcagca cgtccatcag cgtgtcctgg aaggcgtggc
      721 ccatgtgcag cgtgccggtg acgttgggcg gcgggatcat gatgcagtag ggcggctcgc
      781 cgctctgcgc cggatcgggg cggaaatggt cgcccgcctc ccagcgggcg taccactgct
      841 gctccagttg tgccggctga tagcttttgt ccagagtcgt ggtcatggtg gctcgttggg
      901 ttcgatcggc aacgggttcc ggcacggaac agcgcgcatc gggcgatcgg tggaccgggc
      961 agtttagtga gctgccgctg gcttgtgaag cgtgctgtct gtgtctgaaa agagcattga
     1021 tcgagcggca gacaccttca ccgcccaatt gattgatctt gaaattgcaa tgtgctcgaa
     1081 ttcccgctcc atttaactga cgggagcatt catgagcctc atcaaggagt tcaaggagtt
     1141 cgcaatccgc ggcaatgtca tcgacatggc ggtggggatc atcatcgggg ccgccttcgg
     1201 caagatcgtc tcgtcactgg tgggcgatct gatcatgccg ccgatcggtc tgctgctggg
     1261 tggggtcgac ttcagcgatc tggcgatcac cctgaaggcg gcggccgagg gtgcgccggc
     1321 ggtggtgatc agctatggca agttcatcca gaccgtgatc gacttcatca tcatcgccgc
     1381 tgcggtcttc gtcggcgtca aggcgatcaa ccagctgcag caacagaagc aggccgcgcc
     1441 aaccgcgccc tccaacgaag agaagctgct gagcgagatt cgtgatctgc tgaagagtca
     1501 gtcacgctga ttttgccgca tcagctgcct gcggatacga gctgggctgt ccttgtcctg
     1561 cgggccggct gccgatggac agacatctgg gccgtggtgg gcagcgtccg gactgaaaca
     1621 tgaatgtcat gcggtgcagt cacgctgtga ccgtgctgtc atgaacacgg gcgtcagtca
     1681 tcgcaatgtc aactctgcgg aatccagaaa cccttccaga ccgtcatctg cttgccgaga
     1741 tcggcaatca gctcgaacag ttgcgccaag cggtgatcgg tgacgccgct cggctccaga
     1801 gcgcctcccg tggcgacgac gacaccccca gcctgagaaa ctttgcccat tatctggcgc
     1861 tgcgcagcca cgacctgcgg ccgctgcagg cgctgcttgg gcagtgtgca ctctcgtcgc
     1921 tggggcgcag cgaagcccac gtcatggcga ctctcgactg tctgctggcg ctgatcgctg
     1981 ccgtgctggg ccggtcggaa ccgatcgagc ccgccgcacc ggtgacattc gacatgggca
     2041 gacagttgtt gtctgccaac cgcatacgtc tgttcggccc gttgccgagc gaccgcccca
     2101 gccacatcat ggtgaccatg ccgcgggcag tggccagcga ccccggcctg gccatgcaac
     2161 tggtcgccgc cggcatggac tgcgcgcgga tcaactgcgc ccatgatgat gccggaatct
     2221 gggaggcgat ggtgcgcaac ctgcgcgccg cggcgctggc tgcgggccgc gaatgccgga
     2281 ttctgatgga tctggccggt ccaaaattgc gtaccgggcc ggtggcattg gccgattgca
     2341 tgcggctgaa gggtgtggtg atgctggtgt cgggggtacc aaccgaagcc ggtctgctcg
     2401 ggtttccggc ggaattgatc gcggcactgg cacccggaga tgtcatcacg ctgcgcgatc
     2461 gacgggcaaa gaaacgcgaa ttgctgatcg agggcaggga gccggacgga cgttgccgcg
     2521 cgctgtgcga gcgcgccgcg cagatcgacg cgcagaccga gttctgcatc acccttggcg
     2581 gacagcggag gcaggcacgg gccgccatgc agatgccgcc ggtcgagatt cgcctgttcg
     2641 aaggcgattc gttgctgctc gatcgggcag cgcgtcctgg tgttgcggca cagcccgatg
     2701 gcaccccggc acgcatcggc tgtggtgagc ctgccgtgct ggatcagttg accgcgggca
     2761 tggcggtctg gatcgatgac ggcaagatcg gtgccctggt cgaacgaatc gatgccgagg
     2821 gcgcgttgct gcgcatcacc gcggcagggc cgaaggggcg tcgtctgcgc gccgaccagg
     2881 ggctgaactt tcccgattcg gccttgcggt tgccggcgct cagtgacaag gatcgccgtg
     2941 acctcgactt tgccatccac catgccgatc tgatcgggct gtcgttcgca cagtcgcgcg
     3001 ccgatctgga agcgctgcgc acggcgctgg ccgagcgtgg cgcgccgctg ctgccgatca
     3061 ttgccaagat cgagaccgcc gccggtgtcg gcaacctgcc cgagatgctg ctggcgatgg
     3121 tcgatcgcca cccctttggg gtgatgatcg cccgtggcga cctgatggtg gagatcggtg
     3181 gcgagcgaat ggcggagttg caggaagaga tgctgtggct ctgcgaggcg gcccatgtgc
     3241 cggtgatctg ggccacccag gtgctggaga gcctgaccca tgacggcagc ctgtcacggc
     3301 cggagctgac cgatgccgcg gcctcggccc gcgccgactg cgtgatgctc aacaagggcc
     3361 cacatctgct gcgtgcgctg tcggtgctgg tcgatatcca cacccgcatg gccggccatc
     3421 aacagaagaa aaatccgcaa ctgcgcgcgc tgtcgctggc cagccggttg ctggagaacg
     3481 gtgacgggcc cgccgcgtga ggtgggtcat ccgctgtcgc gctgacgcat cacctcgtac
     3541 agcagcagtg cgctggccac cgagacattg aggctggcca ccgcgccctt catcggaatc
     3601 gccacctcaa agtcgcactg ctcgcgggtc agccgtcgca ggccatggcc ctcggcgccc
     3661 atcaccagtg cgatcggacc cttgaaatcg gcctgaaaca gcgtgccggg gccatcggcg
     3721 gtggcggcca ccagccagag gccctgtgcc ttgagcgccc ccagcgtgcg tgccagattg
     3781 gtgacccgaa agcagggggt cagttcggcc gcgccggagg cggccttgcg tgccacggca
     3841 ttcagtggtg ccgagcggtc cttgggcagc acgatgccat cggcaccggc ggcattggcc
     3901 gtgcgcaggc aggcgccgag gttgtgcgga tcggtgatgc catcgagcac cagcaggaag
     3961 gccggtcgtt gcaggccatc gagcagggcg aacagctcat tttcgccggc ctcgacactg
     4021 gtctcgacca gcgccaccac cccctgatgg acgccgggcg ccagttggtc gagttcgcgg
     4081 cgtgagcgct gttcgatggc aatgccctgc gccctgagct gcttgatcag ggcctgcagc
     4141 ttctcttcgc ggccctgctg cagcagcacc cggctgatgc gggtgggatc actgtgcagc
     4201 agtgcagtga cggcatggac gccatagacg gtctggctca tcggcggtga actcatgcag
     4261 ggtggggcgt tcgctcagcc cgatttgcgc cgacgacgct tgccgccgcc ggtgccgtcc
     4321 tgagtggcct tgttcttctt gccgcccttg ccggaaccag gcgtggcggc ggggcgttgc
     4381 agcaccggtt cgaagtcgat ggtgcgctca tcgaggtcga ccctgaccac cttgacccgc
     4441 agcgggtcgc cgaggcggta gaccgcgcgg ccgcgctcgc cgaccaggcg atgcttgctc
     4501 tcctcgaagt ggtagtagtc gttgggcagt gcggtgatgt gcaccagccc ctcgacgaag
     4561 atgtcgcgca gttcgacgaa gaggccaaag gccaccacgc tggtgatcag gccgtcgaat
     4621 tcgctgccga catgcgcgcg caggtattcg cacttcagcc agtcgaccac gtcgcgggtg
     4681 gcggcgtcgg cgcggcgttc ggtctgggag cagtgctggc cgatcagcgc cagcccctgc
     4741 ggatcgtaag ggtagacctg ctgctttctg agcacgccgg cgcccttgac ccgccgcagg
     4801 tgctgggccg gcttgcggct gcgcagcgtg gcacggatgg cgcggtgcac cagcagatcg
     4861 gggtagcggc gaatcggcga ggtgaagtgg gtgtaggcct cgtagccgag gccgaagtga
     4921 ccgaggtttt ccggctggta gagcgcctgc cgcatcgagc gcagcatcac catctggatg
     4981 tggtgggcat cgggccgcgc ggcgatggtg tcgagcagct tctgcatctg cttcggtgac
     5041 ggctttttgc cgccggagag gctcaggccc agttcggcca ggtaggcgtg cagccgggtc
     5101 agcttggttt cgtccggcac ctcgtggacg cgaaacagcg ccggaacctt gtggtgcagc
     5161 agcaggcggg cggcgcagac gttggcggcc agcatcgcct cttcgatcag ccgatgggcg
     5221 tcgttgcgca ccactggcac gatgcgctcg atcttgcgct cggcatcgaa caggatgcgg
     5281 gtttcgaggg tgtcgaaatc gatggcacca cgcgcccggc gcgcctgatg cagcacccgg
     5341 tagagcgcat ggaggttctc cagttgcggc agcagcgggc ggtgtggcga ctgctggcgc
     5401 agtggcgcct ctgccggtgc ggccagcaac tcggccactt cgctgtaggt gaggcgggca
     5461 tgggagtgga tgattccttc ataaaaacag aaatcatcga tctgcccctt gcggtcgatc
     5521 accatctcgc agaccagcac cagacggtcg accctgggct tcagcgagca gaggccgttg
     5581 gagagcgcct ccggcagcat cggcaccacc tgggagggaa agtagaccga ggtgccgcgc
     5641 tcgtgggcgg cggcatcgag cgcgctgccg ggcgtcacat agtgcgcgac atcggcaatc
     5701 gcgacccgca gcagaaaacc gccgtgggcc tggcgctcgc agagcacggc atcgtcgaag
     5761 tcgcgggcat cctcgccatc gatggtgacg aacggcaagc tgcgcagatc gacccggcgc
     5821 gtcagctcat cggccgcgat cgaatcgccg agccgcgccg cttcgcgctc gacctcttcg
     5881 ctccagcggt gcggcaactg gtggctgcgc agcgcgatct cgatctccag ccccggcgcc
     5941 agatattcgc ccagcagttc gaccacccgc ccctgtggca gctgccgtgg accgggctgc
     6001 tggatcagct cgatcatcac atagttgcca tggcgcgcat gaccggcttc gccagggggg
     6061 atcagaatct catgctggat gcggcgattc tccgcccgca cgaaggcgac gccgcgctcc
     6121 tcgaaatagc ggccgaccaa cgcccaggtg cggtgctcga ccacctcgac gatcaccccc
     6181 tcgggacggc cgcgacggtc ttcgcccacc ttgcgcacca gcaccaggtc gccgtcgaac
     6241 accttgcgca tctcgcgtgc gccgaggtag aggtcggctg agccatcgcc cggcaccgca
     6301 aagccgaagc catccttgtg gccctgcacg cgggcggcga tcgaatccat cttgtcgacc
     6361 ggcaggtagg cgtcacggcg gttgcggatc agttgcccat cgcgctccat cgcgatcagc
     6421 cgccgccgca aggcctcgac atcatcggcc gaggcgatgc ccagctcttc gcagagccgg
     6481 gcgtggctgg ccggcccttt gcgttcgctc agccacttga ggatgaattc acggctgacg
     6541 atcggatggc tgtacttctg ctgctcgcgc gccagaaaag gatcgtcgtt gccggggtgg
     6601 cctgccattg gagtgacctg aaagagatga aaaatctgct ggttcgcccg gcattgtggc
     6661 ggttgaccac gatcaagaaa agggtgcggt ggcaggaggc aaggatgata aagcgtcaaa
     6721 atccactttt tttttggtat tatggcgcca cagaatcgac ggttctgcaa cccgagtctc
     6781 tccctcaaac attgtcggca acgacaggat ggcgaaccat gaaacttccg acccacgccc
     6841 gcatcgcggc ggcggccctg ctctgcatcg gcctgccgca gcttgctcaa gccagtgccg
     6901 tctatggcac gctcagcaac ttcgatgtct acaatgattc gaccacaccc taccacggct
     6961 tcgagatcga actcgaggat ttctcgctgt cgcagcttgc cggctacaac ggcaactact
     7021 acacctaccc caactggcac tatggcagcg gtcaggtgag ccagagtggc accaatgtgc
     7081 tcatccgcta cagcaaggcg ggtgggcaga ccgccccgtt cagcggcaac gtcgtcgcca
     7141 ccaatggcca tgcctgcatc accatcgctg gctgtgaaca cttcggtgtg tcgctcaatg
     7201 gcgcgcccac cgccacccgc tacttctggc tcgacgagaa tggcaaccgt tcgacccagg
     7261 tcaatctgct ggcaccggtg atgacggtgg tgcagcctgc ggtgccggcg cagaacggca
     7321 acccgccgca gcccgccatc gttcgcgccg aagtcgaggc gccggaggtg atcaaggcca
     7381 atggccgttc cgacgcgatc tgggtgcagg tgttcaagcg cgaggtcgac aatgatcagg
     7441 agggcgaggt cgaactggat gatctgatgg ccgacaatgg cggcgtcgtg gccgatgagg
     7501 gcgatggcgt cgaaatcgag tggaagttgt tccagcgcaa gccgggagac gactctgcca
     7561 acctgctcga cagcggcgag caggaggtgg gcgatggcat ggagcaggtg ctgcgcacct
     7621 accgcttcta caagttcgct ggcacctatg atgcaagcca cgaggccacc tgcaccgaag
     7681 tgggcaactg tgaagatctg gcggaagcag gagagcatat ggactatgtc ggcgcgctga
     7741 tcggccagca gatggtggcg ctcaacctcg atggcgccct gccgccggcg ccggtgccgc
     7801 tgccggcctc ggtctggctg ctgggttcag gactgttcgg actcaccgcg ctggcgcgcc
     7861 gccactccag gggcgtctga gccgttcgcc attgctgaag gcacagggag gtgccttttg
     7921 ttttttgttc gatcactcgg ctgcggctgt ttcacggcgt acgcacacgg cctcggcgtg
     7981 atcgcttcga caccccggcg cagcaaaaac cactccgaga accacaactg ccggagcagt
     8041 gcggactctt gacgtgatgg gcggcgcttc tataatgcgc cctccctgtc atgtcggcgc
     8101 cggtagctca gttggataga gtatttggct acgaaccaaa gggtcggggg ttcgaatccc
     8161 tcccggcgcg ccatgtcagc ggatttagac tgagtcactc ttgcggcgaa gccggtcaga
     8221 tcggctccag ccgcgcatag gccagcacca gccacttctc tccttcgcgg tcgaagttga
     8281 cccgcacccg ggtctggttg cccgatccct cgcagtcgat caccgtgcca tcgccaaacc
     8341 ggccgtggtg cacccgcgcg ccgagttgca atccctgctc ttcgccgaca tcggtcctga
     8401 tctggccgtg gccgacgctg ccgccgctgc tggcctgttg cagcaggtgg ggcggaatct
     8461 cgcgcacgaa gcgcgacagg cggttgtggc agagggtgcc atgcagccgg cggctctcgg
     8521 tgtggctcaa gtagagcttg tgcatggcgc gggtgagacc gacgtagcag aggcgccgct
     8581 cctcttcgag ccggccgggc tcttcgagcg acatctggtg cgggaacagc ccctcttcga
     8641 tgccgacgat gaagaccacc ggaaactcca gccccttggc cgagtgcagc gtcatcaact
     8701 gcaccgcgtc gagcacttca ccgccctgtt ccgcaccggc gtcgagcgcg acgctgtcga
     8761 gcagcagcgc cagttcgtcg tgggcggcag tggcgaggag ggtctcatcc tcactgcgca
     8821 gcagttcgct gaactggccg ctggcgctca ccagctcctg caggttctcg acccgaccgc
     8881 gccccttttc accccgttcg ctggcatgaa actcgatcag cccgctgcgc tggatcacct
     8941 gctcgacccg ttgcgacagc gccagatcgc gggtcgcccg gtcgagttca tcgatgttct
     9001 gagtgaaggc ggccagcgcg gtgagggcgc gggccggcag cgcgcgggcg gtgaccatct
     9061 gctgcatggc gcgccacagc gaccactgtt gccctctggc ctcgctgcgc agcaactcca
     9121 gggtcttgcc gccgatgccg cgcggtggcg cggtcagcac ccgctccagc gaggtgtcgt
     9181 cgtggcggtt gagcatcagc cgcagatagg ccagcgcatt cttgatctcg agccgttcgt
     9241 agaagcgctg gccgccatgg atgcggtagg cgatgccggc gcgcagcagt gcctcttcga
     9301 gcagccgtga ctgggcattg gagcggtaga gaatggcgat ttcgcgacag gccagcccct
     9361 gggcgagctg ctggcgaatc tggccgacga cgaagcgcgc ctcatcctct tcgttgtagg
     9421 cggtgtagag cgcgatcggc tcgcccgggc tgccgtcggt ccacagtgcc ttgcccagcc
     9481 gttcgcggtt gcgggcgatc accgcattgg cggcatcgag aatggtggcg gtggagcggt
     9541 agttctgctc caggcggacg gtctggacat cggcgaagtc gcggctgaaa cggtggatgt
     9601 tctcgatgcg ggcgccacgc caaccgtaga tcgactggtc gtcgtcgccc accgcggtga
     9661 cccgggcacc accgccggcc agcagccgga tccaggcata ctggatgctg ttggtgtcct
     9721 ggaactcatc caccagcaga tggcgaaagc ggttctggta gtggtgcagc agttcggggt
     9781 ggttgagcca cagctcatgg gcgcgcagca gcagctcggc gaaatcgatc acgttggcgc
     9841 gctggcaggc ggcttcgtag gcctggtaaa tctcgaccag cgtggccagt tgccggtcgc
     9901 cctgcggctc gatccggttt ggtcggatgc cctcatcctt ctgcgcattg atgaaccact
     9961 gcgcctgccg ggccggccac tgctcctcgt tcaggcccaa ctcgcgcagc gtgcgtttga
    10021 tcagccgctg ctggtcatcg ctgtcgagaa tctggaaatc ctcggcgagg ccggcatcgt
    10081 gccagtgcag gcgcagcagc cgatgggcca ggccatggaa ggtgccgacc cacatctggc
    10141 ggatgtcgtg gccgagcagt gtctcgatcc gctcgcgcat ctcccgcgcc gccttgttgg
    10201 tgaaggtgac cgccaggatt gcatgggggg gcacctggtg ccgttggatc agccaggcga
    10261 tgcggtgcac cagcacctgg gtcttgccgc tgccggcgcc ggccagtatc agttggtggg
    10321 tggcggatgc ccgcactgcg tcgcgctggg cgggattgag ctggtcgatg ggcgtggagc
    10381 tgttcatcgg gcgattgtag caaagatgcc tccatcgccg ggctgcgctg ccggcccgga
    10441 cgcttgggcc atgcgcggca tgggatgccc caaggttggg cagggcttgg aaaagggggt
    10501 gtcgactggg tagaatgtac agccaatgga gcgggccgtg ctggccccgg ccgagatgga
    10561 tgccgcggcg gcttcccgac ctccgggtcg cgacgaacaa gaaaaactcc cgccctgtcg
    10621 cgtggtcagg gcggtcgcac aacaaggatt ctcatgtccg catccaccca actgctggcg
    10681 cacaactggt cattcggcat ctatctgttt gcgatcgccg gggtctgtgc gctgatgctg
    10741 ctgctctcct ggctgctggg cggccgcgcc tgggggcggg cgaagagcga ggccttcgag
    10801 tcgggtgtgg tgtcggtcgg tggcgcccgc ttgcgcttct cggccaagtt ctatctggtg
    10861 gcgatgttct tcgtcatctt cgatgtcgaa gcgctgtttc tctatgcctg ggccatttcg
    10921 ctgcgcgaga gcggctgggc gggcttcgtc gaggccacca ttttcatctt cgtgctgctg
    10981 gtgggtctgg tctatctgtg gcgcatcggc gcgctggact gggccccgca ggggcgcggt
    11041 caaagagcgc aacccagggg tgagaaatag cgatggacta cacgctgacg cgggttgatg
    11101 aagagcagca gcgctatccg ctggagcagc gcattccggt ggccgacccg ctcgagcagc
    11161 atgtgcacaa aagcatctac ctgggcaagc tcgagcaggt gctgcacaat gccgtcaact
    11221 gggggcgcaa gcactccctg tggccctaca acttcggcct ctcctgctgc tatgtggaga
    11281 tggcgacctc gttcacggcg ccgcatgatg tggcccgttt tggcgcggag gtgattcgcg
    11341 cttcgccacg gcaggccgat ttcatggtga ttgccggcac ctgcttcatc aagatggcac
    11401 cggtggtgca gcggctctat gagcagatgc tggagccgaa atgggtgatc tcgatgggct
    11461 cttgtgccaa ttcgggtggc atgtatgaca tctactcggt ggtgcagggt gtcgacaagt
    11521 ttctgccggt cgatgtctac atccctggct gtccgccacg tcccgaggcc tttctgcagg
    11581 ggttgatgct gctgcaggag tcgatcggcc gggagcgccg gccgctctcc tgggtggtgg
    11641 gcgagcaggg cgtctatcgg gcagaagcgc cctcgcagcg cgagttgcgc cgtgaggagc
    11701 gcatgagcat gaccacgctg cgcacgccag accaggtgta gtccggtgca cgttttcttc
    11761 aggaacagct accaagtgag ctctcgatga gcgacccagc gctcgccgca cagatggcat
    11821 ccggggacca gcatcaggtg gtgcgtgcgc tgcgcgcacg ctttgccgat gtcgagctgg
    11881 tttttcagcc caccctcgat gaactcccca cgctgtgggt ggcgcgcgag cggctgctcg
    11941 acctgctgcg ccatctgcgg cagctgccgc aaccctatgt gatgctctat gacctgagtg
    12001 cggtcgacga gcggctgcgc acgcagcggc ccgggctgcc gaaggccgat ttttcggttt
    12061 tctatcatct gctgtcgatc gagcgcaaca gcgatctgcg catcaaggtg gcgctgtcgc
    12121 aggatgatct gcgtctgccg agcaccgtgt cgatctggcc caatgccaac tggtatgagc
    12181 gtgaggtgtg ggatctgttc ggcatcgagt tcgatggcca tccccatctc gagcggatca
    12241 tgctgccgcg gacctggagc ggccatccgc tgcgcaagga ctatccggcc cgcgccaccg
    12301 agttcgatcc cttcatgctc gatgcggcca agcaggatct ggagcaggag tcgctgcgtt
    12361 tcgtccctga agagtggggg atgaagcgcc attcgagtga tgccgacttc atgttcctca
    12421 acctgggccc caaccacccc tcggcccatg gtgctttccg catcgtgttg cagctcgatg
    12481 gcgaggagat cctcgactgc gtgccggaca tcggctacca ccaccgtggc gccgagaaga
    12541 tggccgagcg gcagagctgg cacagctaca ttccctacac cgaccgcatc gactatctgg
    12601 gcggggtgat gaacaacctg ccctatgtgc tggcggtcga gaagctggcc ggcatcgagg
    12661 tgccggatcg ggtcaagacc atccgggtga tgatggcgga gttcttccga atcaccagcc
    12721 atctgctgtt cctgggcacc tatatccagg atgtcggcgg catgacgccg atcttcttca
    12781 tgttcaccga ccgcatgaag gcctatgacg tcatcgaggc gatcaccggc gggcgcatgc
    12841 atccggcctg gtaccgcatt ggtggcgtgg cccatgacct gccgaccggc tgggagcgct
    12901 tggtgcggga attcgtcaac tggctgccga agcggctcga cgaatatgag aaggcggcga
    12961 tgcgcaacgg cattctgcgt gcgcgtacca tcggtgtggc ccagtatgac accgctgcgg
    13021 cgctggcctg gggggtgacc ggggctggcc tgcgtgccac cggccttggc tatgacctgc
    13081 gcaaggcgcg cccctattcg ggctatgaga atttcgaatt cgaggtgccg gtggcggcca
    13141 atggcgatgc ctatgaccgg gcgatggtgc gactcgaaga gatgcgccag agtctgcgca
    13201 tcatcaagca gtgtctcgag cagatgccgg ccggccccta caaggccgac catccgctga
    13261 cctgcccacc gcccagggag cgcacgctcc aggacatcga gacgctgatc acccactttc
    13321 tgtcggtctc ctgggggccg gtgatgcctg ccggcgagtc gagtcagctg atcgaggcga
    13381 ccaagggcat caacagctac tacctgacca gtgacggggg caccatgagc taccgcacgc
    13441 ggattcgcac gcccagcttc ccgcacctgc aacaaattcc gtcggtgatc cgcggcagca
    13501 tgatccccga cctgatcgcc tatctggcaa gcattgattt cgtgatggcc gatgtcgacc
    13561 gataacccca actcaacttc ggcgcagcgc ctgaccagcg agcgccatcc cggaggcttc
    13621 gagctcagcg cagccgagcg ggcagcgatc gagcatgaga tgtcgcacta tgaagatccg
    13681 cgcgccgcat cgatcggtgc gctcaagatc gttcagcagg cgcgcggctg ggtgcccgat
    13741 ggcgccattg ccgccatcgg taacctgatc ggcatcccgg ccagcgatgt cgagggggtc
    13801 gcgaccttct acaatctgat cttccgccag ccggtggggc ggcatgtgat caagctgtgc
    13861 gacagcatcg cctgctttct gaccggctat gacgagctca aggcggcgct gaccacccat
    13921 ctgggcatcg acttcggtca gaccacggcc gatggccgct tcaccctgct gccgatctgc
    13981 tgtctgggca actgcgacaa agggccgacg ctgatgatcg atgatgacct gcatggtccg
    14041 gtcgcgcccg ggcaggtggc cgcactgttg gagggttacc cgtgagcggt gcgatggcgc
    14101 gcagtgccga gacccatccg ctcacctatc ggctggagag cggtgagccg gtggtcgacc
    14161 tcaaggcgta tgaggcgctc gctggctatg ccgccctgcg caaggcggtg ccggcgatga
    14221 cgcctgccga ggtggcccag gtggtgaagg atgccaacct gcgcggtcgg ggtggcgcgg
    14281 gctttcccgc cggcatcaag tgggggctgg tgccgatggg cgccgatgcg ccggcacaga
    14341 aatatctggt ctgcaatgcc gatgagatgg aacccggcac cttcaaggac cggctgttga
    14401 tggagaaact gcctcatcag ctgatcgagg ggatgatcgt tgccgcctat gcgatccagg
    14461 catcacgtgg ttacatcttt ctgcgcggcg agtatgtgct ggccgccgag cggctcaacc
    14521 gggcaattgc ccaggccatc gaggctggat atctcggcca gaacattctc ggctccggct
    14581 ggagcttcga tctgttcgtc cacaccggtg ccggccgtta catctgcggt gaagagactg
    14641 cgctgatcaa ctcgcttgag ggtcggcgcg ccaacccgcg ttccaagcca ccctttccgc
    14701 aaatcgccgg tttgtggggc aagccgaccg tggtcaacaa ttgcgagacg ctcaacaatg
    14761 tcgccccgat cgtgctacat ggggccgact ggttcaaggg actgtcgcag ggcaagagtc
    14821 cggatggcgg taccaagttg tatggcgctt ctggccgggt gaagcggact ggggtctggg
    14881 agttgccgat cggcaccacg gcgcgggaga ttctcgaaca gcacgccggt ggcatgcagg
    14941 atggcctggc gctgaaggct tggctgcccg gcggggcctc gaccgacttc ctgctgccca
    15001 gtcacctcga tcttgcgatg gatttcgaca ccatcatgaa ggccggtagc cgcatgggca
    15061 ccgggctgat catggcgatc gatgatcggc agagcatggt ctcggtggtg cgcaatctgg
    15121 agatcttctt cgcccgcgaa tcctgcggct ggtgcagccc ctgtcgcgat ggcctgcctt
    15181 ggacggtcaa gatcctctct gccctcgagc gtggcgaggg tcaggccggc gagatcgaga
    15241 ttctggagca gatcgcccgt caactgggtc cgggcaagac cttctgtgcc cacgcgccgg
    15301 gagcggtgga gccgttgcag agtgcaatca aattttttcg cgaagagttc gagcgcggca
    15361 tcgcggcgcc agaggccgag cgggcctgat ggcgtgacga tgcggccact tttcgacagg
    15421 gctggataga gagacgcatg gcaacgattc atgtagatgg caaagagtac gaggtcgatg
    15481 gcgccgacaa cctgttgagc gcctgtctct cgctgggcct caatgtgccc tatttctgct
    15541 ggcatccggc gctcggcagc gtgggcgcct gccgccagtg cgcggtgaag cagtatgcca
    15601 atgccgatga cagccgcggc cggctggtga tgtcctgcat gaccccggcg gccgacaaca
    15661 gctacatctc gatcgatgac gaagaggcca ggcagtttcg cgccagcgtc atcgagtggc
    15721 tgatgatcaa ccatccgcat gactgtccgg tctgcgaaga gggcggcaac tgccacctgc
    15781 aggacatgac ggtgatgacg ggccatgacc ggcgccgcta ccgtttcacc aagcgcacgc
    15841 acccgaacca ggagcttggc cccttcatcg gtcatgagat gaatcgctgc atcgcctgct
    15901 accgctgcgt gcgctactac cgcgactatg ccgagggcga ggatttcggt gtctacggcg
    15961 cccatgacaa cctctatttc ggccgcctcg aagagggggt gcttgagaac gaattctccg
    16021 gcaatctggt cgaggtctgt ccgaccggtg tcttcaccga caagacccac tcggcccgtt
    16081 acaaccgcaa gtgggacatg cagttcgcgc ccagcgtctg ccagcactgt gcggtgggct
    16141 gcaacatcag ccccggcgaa cgctatggcg agttacgacg ggtcgaaaac cgctacaacg
    16201 gtgcggtcaa ccactatttt ctctgtgacc gtggccgttt cggccatggt catgtcaaca
    16261 gcgccgaacg gccgcgcact ccgctgctgc gtcagggcga tgccttggta tcggccacca
    16321 tcgacaccgc attgaccacg gccggcgaag cgttgcgcca cgtgcgcaga atcatcggca
    16381 tcggctcgcc gcgcgccagt gtggaggcca acttcgcgct gcgtgaactg gtcggtgctg
    16441 acaacttctc ggttggcatg gccgatcagg agtggcgtgt cgtgcgcaag atcctcgacg
    16501 tgctcggcag cacgacggcc gaaattccga cgctgcgcga gatggaagag cgcgatgcgg
    16561 tgttcattct tggcgaggat gtgacccaga ccgcgccgcg catcgcgctg gcattgcgtc
    16621 aggcgaccaa gggcaaggcg cgcgcgctgg ccgcggccaa gaaggccttt gtctggcagg
    16681 atgaggcgat ccgcaacatt ggtcagcagg cgcgtcatcc gctgatcgtc accggggtga
    16741 tcgaaagccg gctcgacgat gctgccagcg aactgcatcg cgacacgccc gaagcgctgg
    16801 cgcagctcgg tttcgcagtg gcgcatgcca tcgatgccga ggcgccggcg gttgcacttg
    16861 ctccggagca gctcgaacgg gcgcaacgga tcgccgccac gctgctggcg gccgagcggc
    16921 cgctgctggt ttctggcagc agtctcggct gcgaagcgct gatcgatgcc actgccaaca
    16981 tcgccactgc gctgcgcagg cagggcaggg cagctgccat cacgctggtg gtgcccgagg
    17041 tcaatacgct gggcgtggcg atgctgggag gggtgtcggt cgatgtcgcg ctggccgaac
    17101 tgcaacaggg tgccgccgat ggattgatcg tgctcgagaa cgacctctac cggcgggtcg
    17161 atgccgcgac cgtcgatgcc gcccttgact cagcgcggat ggtggtgacg gtcgatcacc
    17221 aactgaccgc caccgtggcc cgatcccagg tgacgctgcc ggccgccagc ttcgccgaag
    17281 gcgacggcac cgtggtcagc caggaggggc gcgcccagcg gttctttcag gtctatgacc
    17341 ctggctatta caacctcgcc agccagctga aggagagttg gcgctggctc gaagggctgc
    17401 acagcgtgat cggtggtgag ctgccgggct gggatctgct cgatcagatc accgccgcct
    17461 gtgccgcgaa ggtgccggca ttgcgccgca tcagcgaggc ggcgcccgat gcgaatttcc
    17521 gggtcgcggg ccgcaagatt gcccgccagc cgctgcgtta cagcggccgc acggcgatgc
    17581 gcgccaacct gtcggtgcac gagccgcgcg catcgcagga tggcgactcg gcgctgacct
    17641 tttcgatgga gggctatggt ggaccggccg aacccggcgc gctgattcct ttcgcctggg
    17701 cgcccggctg gaactccccg caatcctgga cccgattcca ggacgaggtg ggtggccatc
    17761 tgcgcggcgg cgatggtggt gtgcgcctgt tcgaagcgtc gacgcctcaa gtcggggagt
    17821 acttccgtga gcagcctgtg ctgccaatct ccgccgaggg tcgcttccgc ctgctgccgc
    17881 tttatcacat cttcggcagc gaagagctct cgattcgggc tccagcggtc gccagccggg
    17941 cgccccaacc ctatctggcc gtggcgcagg ccgatgccag ccgcctgggc attgccgagg
    18001 cggaccaggt cgaggtcacc atcggcaccc ggctgctgcg gctgccggcg cgggtcagcg
    18061 cgacactggt ggccggcacc gtcggtctgc cggcggggtt gaccggtctg ccgccactgc
    18121 cggcgctggg ctggagtgaa ctgaaacggc tcgaaacggg caaggtggcg acggcatgaa
    18181 ctggctcacc cctgatctgc tcaccatcac ccttgccgtc gtcaaggcgg tggtgattct
    18241 gctggtggtg gtgctgttcg ctgcggtcct gagctggatc gagcggcgcc tgctggcgct
    18301 gtggcaggac cgctacgggc ccaaccgggt cggtcccttt ggcgccttcc agatcgttgc
    18361 cgacatgatc aagatgttct tcaaggagga ttggacgccc cacttcgtcg accggctcac
    18421 cttcgtgctg gcgccggtga tcgcgatgag tgcgttgctg atcgccttcg cgatcgtgcc
    18481 gatcagcccc gactgggggg tggccgatct cgagatcggc attctgctgt tctttgcgct
    18541 ggccggcttg tcggtctatg cggtgatgtt cgccggctgg tcgagcaaca gcaaatattc
    18601 cttgctgggc gggctgcgct cggcggcgca gacggtcagc tacgaagtgt tcatggggct
    18661 gtcgctgatg ggcgtggtgg cgcaggccgg ctcgttcaac atgcgtgaga tcgtcgcagc
    18721 ccaggagcag ctctggttca tcgtgccgca gttcttcggc tttatcacct ttgcactggc
    18781 cggcgtggcc gtcgcccacc gcagcccctt cgacctgccc gaggcggagc aggagctggc
    18841 cgccggctac cacaccgagt attcgggtct gaaatggggg atgttcttcg tcggcgagta
    18901 tgtcggcgtg gtgctggtct ccgcgttgct ggtgacgctc ttcttcggtg gctggcacgg
    18961 tccattcctg ccgccggcac tctggttcat tctcaagacg ctgttttttg tcatgctgtt
    19021 catcctgatt cgtggtgcgt tgccacgccc gcgctatgac cagatcatgg cggtgggctg
    19081 gaaggtctgt ctgccgctca ccttgatcaa tctgctggtg accgctgcgg tggtgctcag
    19141 tcagagcggc gtgaatcagg caggatgagg aggccgccat gctgattaaa ctgatcaccg
    19201 gttttggcag ccagctgcgc agcatgtgga tggtgttcgc ccacgccttt cgcaagcgcg
    19261 acaccattca atacccggaa cagaaggtct atctgccgcc gcgctaccgt ggtcgcatcg
    19321 ttctgacccg tgaccccgat ggcgaggagc gctgcgtctc ctgtaacctc tgtgcggtgg
    19381 cctgtccggt gggctgcatc gccctgcaga agaccgaaac cgaggatgga cgctggtatc
    19441 cggagttctt tcgcatcaac ttctcgcgct gcatcttctg tggcatgtgc gaagaggcct
    19501 gtccgaccac cgcgatccag ttgatccccg atttcgagct ggccgaatac cgccgccagg
    19561 atctggtgta tgaaaaagag gatctgctga tcgccggctg cggcaagaac cccgattaca
    19621 acttctatcg gcaggccggc atggccattg ccggaaaacc caagggcgcg gccaacaacg
    19681 aggcggagcc gatcagtgtc aagacgttgc tgccatgaat cgtggcagcg gcacgcaacg
    19741 ggtctgagga gcgactgtgg aactggcttt ctatctctcg gcagccgtcg cgctgctggc
    19801 cacgctcggc gtcatcatcg cccgcaatcc ggtgcatgcg ctgctctatc tggtggtgtc
    19861 gctgctggcg gtggccatga tctttttcgc catcggtgca ccctttgccg gcgcgctgga
    19921 ggtgatcgtc tatgccggtg cgatcatggt gctgttcgtc ttcgtggtga tgatgctcaa
    19981 tctcgggccc agcgtcaccg aacaggagcg cctctggacc cagccgcgcc tctggatcgg
    20041 gccgagtctt ctgagtgcgc tgctgctggc tggattgctg agggtgattc tggccgatgc
    20101 cagccagttg gccggtctgg agagcgtggg ggccaagcag gtgggcattg cactgttcgg
    20161 gccctacctg ctggcggtgg agttggcctc gctgttgctg ctgggcgccc tggtggcggc
    20221 ctaccatctg gggcacatcg agagcgataa agacaaaaaa tagattcggg ttggtcggtg
    20281 gctgccggct gaaaccggca cggagcgtgg ctgacgagtc gtggagagtg ttgcaatgac
    20341 aggaataccg atggcgcatg gtctggcttt gtccgccatg ctcttcaccc tcggattggc
    20401 cggcctgatg gtgcggcgca atctgctgtt catgttgatg tgtatcgaag tcatgatgaa
    20461 cgccgctgcg ctcgccttcg tgatggcggg gtcgcactgg ctccagccgg atgggcagat
    20521 catgttcatc ctgatcctga ccctggccgc tgccgaggcc tccattgggc tggcggtgct
    20581 gattcagctc taccatcgcc accacacgct cgatatcgac gttgccagcg agatgcacgg
    20641 atgaacctgt tgttcctgac cattcttttt ccgctgctcg gctatctgct gctggcattc
    20701 agccgtggcc gctggtccga gcgtcaggtg gcgctggtcg gcgtgggttc ggtcgcattc
    20761 gcggccgggg tggccggttg ggtgggcgtc gagtttctgc aacagccgcg tggcgtgatt
    20821 ggctacacgc agttgctgtg gaactggatc gaagcgggca acttcaagtc ggacttcacg
    20881 ttgcggctcg atggcctgtc gctgaccatg ctcggcgtgg tcagcggggt cggttttctg
    20941 atccatctgt tcgcctcctg gtacatgcgc ggtgaagagg gctactcgcg cttcttcgcc
    21001 tacatgaacc tgttcgtggc cagcatgctc tgtctggtgc tgggcgacaa cctgctgctg
    21061 ctctatctcg gctgggaagg ggtcggcctt tgcagctacc tgttgattgg cttctactac
    21121 aaggagtcgg ccaatggcgc ggcagcgcag aaggccttca tcgtcacccg tgtcggcgat
    21181 gtcttcatgg ccattgcgct cttcgcgctc tatctccagt tcggcaccct ggacatccag
    21241 accctcctgg cccaggcgcc gcaggtgtgg cagagcggcg gcgccgcgcc gacgctggtg
    21301 gcgctgctgc tgctgggtgg cgcagtcggc aagtcggcac agttgccgtt gcagacctgg
    21361 cttgccgacg ccatggccgg gccgacaccg gtctcggcgc tgattcacgc tgccaccatg
    21421 gtgacggccg gtgtctacct gattgcccgc acccatgggt tgttcctgct ggcgccacag
    21481 gtgcttgaac tggtggcgct ggtcggcacg ctgacactgc tgctggcggg ctgcgcggcc
    21541 ctggtgcaaa ccgacatcaa gcggatcctg gcctattcga cgatgagcca gatcggctac
    21601 atgttcctgg cgctgggggt tggcgcctgg aatgccgcca tcttccacct gatgacccat
    21661 gcctttttca aggccctgct gtttctttcg gccggtgcgg tgatcatcgc ctgtcaccat
    21721 gagcagaaca tcttcaagat gggtggcctg cgcaagaagt tgccactggt ctacgccgcc
    21781 tttctggtgg gtggctcggc gctggcggca ctgccgctgg tgactgccgg cttctacagc
    21841 aaggatgcga ttctgtgggg tgcgctggca tcggggcatg atctgctggt cgtcgcgggg
    21901 ctggtgggtg cctttctcac ctcgctctac accttcaggc tgatcttcat cgtcttccat
    21961 ggcgagagtc agtgcgatgc ccatgccggt catggcttgg cccatggcct gccgctgctg
    22021 tttctgctgc tgctctccac cttggtcgga gcctggatca cgccgccgct gacggcagtt
    22081 ctgcccaccg atcccggtgc ggccggtggc gaagcgaagc atctgctgga gtggatctcc
    22141 ggcggcgtgg cggtggccgg cattgccatg gcggcgctgc tgtttctcgg caagcggcag
    22201 ttcgttgacc gcgtcgccac cgccggaccg ggacgattga tcggccaact ctggctgaat
    22261 gcctggggct tcgattggct ctacgacagg ctgttcgtca aaccctatgt ctggctggcc
    22321 cacctgcatc gcaatgatcc ggtcgatcag gccatcctcg tcatcccgtt tctggcgcgc
    22381 accggccatg atgcactcag tcgggttcag aatggcaggc tgcgctggta tgtggcctcc
    22441 atcggtctgg gcgcggtgct ggtggtcgcg cttctgctgc tcggttaacc aacaggaact
    22501 tcatcggcaa tgattctggt ctggctcttt ctgattccca ccctgggcgg cttgctcgcc
    22561 tggggcggcg agcgttacgg ttcgggcatc ccgcgctggg tctcgctgct gtcgatcacg
    22621 tcggtcttcg tgctttcgct gctgctgtgg caacagggtg actacagcct tggacagggc
    22681 gtctccgcgc agccgcagtg gcagttggag tttcaggcac cctggatcag ccgcttcggc
    22741 atctcgtttc atttcgcgct ggatgggctg tcgctggtga tggtgatgct gaccgccttc
    22801 atcggcatcc tgtcggtggt ctgctcgtgg cgggagatca accgccatgt cggcttcttc
    22861 cacttcaacc tgctgctgct gctgagtggc gtgatcggtg tctttctggc cattgacctg
    22921 tttctgttct tctttttctg ggagatgatg ctgatcccga tgtacttcct gatctcgttg
    22981 tggggacaca agggggccaa gggctcatcc cggatccagg cggcgatcaa gttcttcatc
    23041 ttcacccagg cgggcggtct gctgatgctg ctggcgatcc tcgggctggt gctgacccac
    23101 tactcggtca gcggggagct gagtttcgat tacgaggtgc tgcgtcacgc catcctggcg
    23161 cccgagcttg aatatgcgct gatgctgggc ttcttcatgg cctttgccgt caagctgccg
    23221 atcgtgccgc tgcactcctg gctgcccgat gcccactcgc aggcgccaac cgccggcagc
    23281 gtcgatctgg ccgggctgtt gctgaagacc gccgcctacg gcctgttgcg gttcgtgctg
    23341 ccgatgttcc cgaatgccag ccatgagttt gcgccgatcg ccatcggctt ggcgctggtc
    23401 ggcatcatct atggcgccgt gctggccttc agccagcatg acatcaagcg gatggtcgcc
    23461 tacaccagca tctcgcatat gggcttcgtg ctgattggca tctacgccgg cagcacggtg
    23521 tcgctgcaag gagtggtggt gcagatggtg gcacatgggc tgtcggcggc tgcgctcttc
    23581 atcctctgcg gcatgctcta tgagcggttg cacacccgcg atctgcggca gatgggcgga
    23641 ctctggtcgc ggctgccgct gctgccggcg gtggcgctgt ttttcgcggc ggcatcgctc
    23701 ggcattcccg gcaccggcaa tttcattggc gaattcctga tcctgctggg ttcctaccag
    23761 gctgcgccct acgcggtgat ggcggccacg ggtggtttgg tgctggcgtc gatctactcg
    23821 ctgcggctgg tgcagcgtgc cttttttggt ccagcccaat cttcggagcc gatcgaggcg
    23881 gccaccggtc gcgaaaccgt catgctgctg ggattggtgg tgctgacgct ctggatcggt
    23941 ttctacccgc aacccctgct gaacacatcg gcggccgcca tcggtcaggt tcagcagatc
    24001 tatgccgccg ccattcaagc tgccgaggcc gctgcgccat gaccattgcc ttgaccgatc
    24061 tgctgccgct cgcgccggtt ctgatcacct cggccacagc catcg
//
LOCUS       DAORCQ010000030        24005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1200_length_24005_cov_6.651566, whole genome shotgun sequence.
ACCESSION   DAORCQ010000030 DAORCQ010000000
VERSION     DAORCQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 24005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24005
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_24005_cov_6.651566"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /gene="pnp"
                     /locus_tag="PKI23_04840"
     CDS             complement(<1..1499)
                     /gene="pnp"
                     /locus_tag="PKI23_04840"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNN36301.1"
                     /translation="MNPIRKSFRYGQHTVTLETGRIARQATAAVLVSVDETTVLVTVV
                     GDRKVVPNRDFFPLTVNYQEKTYAAGKIPGGFFKREARPSEKETLTSRLIDRPLRPLF
                     PKGFMQEVQVVCTVMSVDKVNDPDIAAMIGASAAVALSGIPFDGPIGAARVGFDPQAG
                     YLLNPSYAELEKSALDMVVAGTKDAVLMVESEAKELPEDLMLGAVLYAHQEMQVVIMA
                     IEELVREAGKPRWEWQPPAINEPLYAALQAAFADEVAAAYRITDKSQRRDRLGQLRER
                     AIEALIVSEQFAPATAAELNGLFAKLEKKIVRASIVRGEPRIDGRDNHTVRPLFVETG
                     VLPKTHGSSLFTRGETQALVVATLGSIRDGATIDALEGSYRDNFMLHYNFPPYSVGEV
                     GMMGSPKRREIGHGRLARRGIEVVLPTLEEFPYTIRVVSEITESNGSSSMATVCGASL
                     ALMDAGVPISAPVAGIAMGLIKEENGFAVLTDILGDEDHLGDMDFKVAGT"
     gene            complement(1549..1818)
                     /gene="rpsO"
                     /locus_tag="PKI23_04845"
     CDS             complement(1549..1818)
                     /gene="rpsO"
                     /locus_tag="PKI23_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNN36302.1"
                     /translation="MSMSAEEKAAVVKQYQRSEGDTGSVEVQVALLTANIQKLQPHFE
                     QNKKDHHSRRGLIRMVNQRRKLLDYLKRTDNARYTQLIARLGLRR"
     gene            complement(1926..2849)
                     /gene="truB"
                     /locus_tag="PKI23_04850"
     CDS             complement(1926..2849)
                     /gene="truB"
                     /locus_tag="PKI23_04850"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNN36303.1"
                     /translation="MGRQRKARGRQVDGILLLDKPAGITSNQALQRAKAICGVAKAGH
                     TGSLDPLATGVLPLCFGEATKFSHYLLNADKRYQVTLKLGARTTTGDVDGEVIETFAP
                     LQLTQERVLSALSHFRGEIEQIPSMYSALKHQGKPLYELARAGIEVERPPRRVTIHAL
                     ELLRLGEDEIELDVRCSKGTYIRTLAEDLGKALGSGAHVVQLRRIEAGPYEISQTVTL
                     DALAELREQGGYPALDALLLPASSSVSDFPQVKLNETTLYYFRQGQPIMAPQLPSSGW
                     VRIVDGAGSFIGVGEILDDGRLAPRRLVASH"
     gene            complement(2858..3238)
                     /gene="rbfA"
                     /locus_tag="PKI23_04855"
     CDS             complement(2858..3238)
                     /gene="rbfA"
                     /locus_tag="PKI23_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNN36304.1"
                     /translation="MPREFSRTDRVADFIKRELALQIQNEMRDPRARFVTVTDVEVSR
                     DLAHANVHVTVVDQPSPQARQEILQALRGAAGFLRGRLSKVSQMRTTPELHFFYDESS
                     ERGAHLSRLIDQAIAQDRTHGAKE"
     gene            complement(3264..5870)
                     /gene="infB"
                     /locus_tag="PKI23_04860"
     CDS             complement(3264..5870)
                     /gene="infB"
                     /locus_tag="PKI23_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNN36305.1"
                     /translation="MADVTVRQLAEVVGAPVDRLLKQMHEAGLSQTGVDDLVSDEEKQ
                     KLLSHLRRSHGESGGETRKITLQRKEVSTLKLATGQGRGKTINVEVRKKKVYARRDAA
                     EETQPVPSAPPPVAQHEVVPPAPAEERSARERIAEEVQRKKQEEERLRLIEESRQRER
                     EEEARKEAEALRAREAERAASTTTETDAAGVKPEQPAQPIKTDAEAAAARAKTKPVEV
                     KDERARPRSKVKEKEKPAAQIKGARGTLSFDEDDRAGFRRGGKKIPGKNGRGRFAQHA
                     FAKPTETLTREVGIPPTITVADLAQKMSIKAGELIKVMMKMGTMATINQVIDQDVAAI
                     VAEELGHKVKLLSDSALEDELTASLSIEGEAVTRAPVVTVMGHVDHGKTSLLDHIRKT
                     RVASGEAGGITQHIGAYHVETARGMVTFLDTPGHAAFTAMRARGAKLTDIVVLVVAAD
                     DGVMPQTEEAIQHARAAEVPIVVAITKVDKATADPERVQNELVRHGVIPEEWGGDTQF
                     VQVSAHTGQGIDELLEALLLQSEILELTAVPSAPGRGVVVESRLDKGRGSVVTMLVQN
                     GTLRKGDMVLAGPYYGRVRAMMDEAGRPTEAAGPSLPVEVLGFDGTPDAGESFVVVSD
                     EKKAREVALFRQGKYREVKLSRQPAMPRMEEMFESLQAGEVKTLNVVLKADVRGSLEA
                     IQSALADIGTDEVQVRVISGGVGGISETDATLALASKAVLFGFNVRADAKARKIIEEG
                     GVDLRYYSVIYDLLDDVRQALAGMLAPRFREQILGVAEVREVFHSPKFGQIAGCMVIE
                     GVVHRNKPIRVLRDRVVIYEGELESLRRFKEDASEVRSGTECGIGVKNYNDVRAGDLI
                     EVYEKHQIAPTL"
     gene            complement(5913..7406)
                     /gene="nusA"
                     /locus_tag="PKI23_04865"
     CDS             complement(5913..7406)
                     /gene="nusA"
                     /locus_tag="PKI23_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNN36306.1"
                     /translation="MNKEILLVVDAVSHEKAVPPHLIFEALELALAAATKKRYEDEES
                     DIRVVIDQKTGDYQTFRRWQVVADDQVPELGTELTLQEAHEKSPALQPGDVWEEQVEN
                     TLFGRIAAQAAKQVIVQKVREAERGQISAQYLHRVGQLVNGQVKKVTRDHIIVDLGNN
                     AEALLSRDQAIPRESFRMGDRVRSVLTDVKPELRGPQLILSRTDPRMLIELFRLEVPE
                     ISEQIIEIKGAARDPGARAKIAVKTNDRRIDPVGACVGMRGSRVQAVSNELGGERIDI
                     VLWDDNQAQFVINAMAPAEVVSIVMDEDAHTMEIAVAEENLAQAIGRGGQNIRLASEL
                     TGWEINVMSEQAAIEKQEQESVGLLQKFVTLLDVDEEVAAVLIEAGFSTLEEIAYVPL
                     DELLSIEGFDEEIAEELRNRAKDVLLTQAIANEEKLDEVEPAQDLLEVEGMDPSLAAL
                     LARRGIVTREDLAEQSVDDLLEMEEMDEERAGRLILAARAHWFATNE"
     gene            complement(7425..7886)
                     /gene="rimP"
                     /locus_tag="PKI23_04870"
     CDS             complement(7425..7886)
                     /gene="rimP"
                     /locus_tag="PKI23_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNN36307.1"
                     /translation="MNPKTQQLWQLIEPAVQALGCELWGIEDGVHSKSARLRIYIDRQ
                     PGGVTIEDCERVSRQVGALLDVSEMIDGQYHLEISSPGLDRPLYTLEQYRRFIGSRVK
                     IRLRTLFEGRRNFDGRLTGIEGEDVVLMMGEHEYLLPLCAIDKAHIVPEFD"
     gene            complement(8078..8154)
                     /locus_tag="PKI23_04875"
     tRNA            complement(8078..8154)
                     /locus_tag="PKI23_04875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8118..8120),aa:Met,seq:cat)
     gene            complement(8206..8292)
                     /locus_tag="PKI23_04880"
     tRNA            complement(8206..8292)
                     /locus_tag="PKI23_04880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8256..8258),aa:Leu,seq:gag)
     gene            complement(8313..8777)
                     /gene="secG"
                     /locus_tag="PKI23_04885"
     CDS             complement(8313..8777)
                     /gene="secG"
                     /locus_tag="PKI23_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNN36308.1"
                     /translation="MYYGEAVMETIILIIHVLLGVALVALVLIQQGKGASMGASFGAG
                     ASQTVFGSTGGGSLLTRITTGLAMAFFATSISLAVIAKNRADGEANPLHGLPAIEQGL
                     PSATEGGEIPAQGLLPDRSATDSADVKPSDAAPVPAGGQSEELPKVETGPKQ"
     gene            complement(8756..9508)
                     /gene="tpiA"
                     /locus_tag="PKI23_04890"
     CDS             complement(8756..9508)
                     /gene="tpiA"
                     /locus_tag="PKI23_04890"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNN36309.1"
                     /translation="MRPVLLVANWKMHGSRASVDALVGRIVEGVVPDSGTEIVLCPPA
                     LYLWRLEQLLANSTLRLGVQNIHEEAQGAFTGEISAPMVREFGCRYAIVGHSERRQLY
                     AESDELVARKSMAALRHGLKPIVCVGETVTERDAGLTLSVVERQLKAVLERLDSEDAA
                     RIVVAYEPVWAIGTGRSASADEAQQVHGSIRQQLLAWTARGWAVPLLYGGSVKPDNSA
                     LLLSQPDIDGVLVGGASLDAEQFLAICTTAKR"
     gene            complement(9584..10936)
                     /gene="glmM"
                     /locus_tag="PKI23_04895"
     CDS             complement(9584..10936)
                     /gene="glmM"
                     /locus_tag="PKI23_04895"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNN36310.1"
                     /translation="MMSRRYFGTDGIRGRVGQPPITAEFVLKLGWAVGRVFARQHPDA
                     KVLIGKDTRISGYMFESALEAGLSAAGVDIGLLGPMPTPAIAYLTRTFKARAGVVISA
                     SHNPYYDNGIKFFSRQGTKLPDEVELVIEQQIDEPMVTVAPDQLGKAFRISDAAGRYV
                     EFCKSTVPSALDFDRLTLVVDCAHGATYHVATEVFSELGARVIELGTSPDGLNINQGC
                     GSTHPEALRQRVLSEGADLGIAFDGDGDRVIMVDHTGEIVDGDELLYIIAADQARSGR
                     LIGGVVGTKMSNLGLELALEELRIPFARTDVGDRYVTQALGERGWQLGGESSGHILCL
                     AATTTGDGIVSALQVLAAMISSDRTLHEIKQGMCKLPQVMINVQVDAMVDRRALCASE
                     PIRAAERQIMQRLAGRGRLLLRPSGTEPLIRVMVEGEDAAMISQLAQELAALVGGSNG
                     "
     gene            complement(10933..11805)
                     /gene="folP"
                     /locus_tag="PKI23_04900"
     CDS             complement(10933..11805)
                     /gene="folP"
                     /locus_tag="PKI23_04900"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003197708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNN36311.1"
                     /translation="MGATSRVIDCAGRRLDLAAPCVMGILNITPDSFSDGGRLYRSSS
                     GVQLDPLLATAQQMLAEGAQLLDVGGESTRPGATEVSLQQELDRVIPVVERLTAEFDT
                     IVSIDTSRPELMREAARHGAGLINDVRALRQPGALAAAVELELPVCLMHMQGDPQTMQ
                     QAPGYQDVVEEVRIFLAQRVAQCEAAGLPRSRLIIDPGFGFGKTVAHNLTLLQHLDHL
                     AELDLPLLVGLSRKSMIGAILDRPVETRLHGTLAAQVVALERGARLFRVHDVAPAVDA
                     LRVAWAIFNENVDR"
     gene            complement(11891..13819)
                     /gene="ftsH"
                     /locus_tag="PKI23_04905"
     CDS             complement(11891..13819)
                     /gene="ftsH"
                     /locus_tag="PKI23_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNN36312.1"
                     /translation="MAKNLILWLIIAAVLLTVFNNFSIQPDMQKVSYSQFIEELNQDR
                     VARISIKGDGYTIVGQRKNNGGQFEVTRPNMPDLKLMDELLEHGVEVEGQQPERQSIW
                     MQLLLASFPILIIIAVFMFFMRQMQGGAGGRGGPMSFGKSRARLMSEDQIKTTFADVA
                     GVDEAKEDVKELVEFLKDPGKFQRLGGRIPRGVLMVGQPGTGKTLLAKAIAGEAGVPF
                     FSISGSDFVEMFVGVGASRVRDMFEQAKKRAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNDGVIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDIRGREQ
                     ILKVHMRKVPLGDDVDPAVIARGTPGFSGADLANLVNEAALFSARAGKRKVDMDEFEL
                     AKDKIMMGAERKSMVMSEKEKLNTAYHEAGHVIVGRLVPEHDPVYKVSIIPRGRALGV
                     TMFLPEEDRYSHSKRSIISQICSLFGGRVAEELILGPDGVTTGASNDIQRATDIARSL
                     VTRWGLSEKLGPLMYDEDSTEVFLGRSSGSQQRSISPETARKIDDEVRSIIDSCYDRA
                     RTLLTENIDKLHAMAEALIKYETLNLEQIDDIMSGRPPREPESWHSDRDAAPPRAKSP
                     TSEEKSGKGSIGGTAGEH"
     gene            complement(13975..14613)
                     /gene="rlmE"
                     /locus_tag="PKI23_04910"
     CDS             complement(13975..14613)
                     /gene="rlmE"
                     /locus_tag="PKI23_04910"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNN36313.1"
                     /translation="MARSRSSGQWLREHFDDPYVKQAQKLGYRSRASFKLLELVEKYR
                     LLRRGMTVIDLGAAPGGWSQVAMQMTGEEGRVIAIDRLPMEPIGGVEFIEGDFTEAAA
                     LDQLRRVLGGQPVDLVLSDMAPNSSGNRAVDQPRAMELAELALDFAQQALTPGGAFIA
                     KLFQGEGFDGYLGNCRTTFEQVRCVKPKASRPRSPEVYLVGTGFRGQRPVSG"
     gene            14722..15039
                     /locus_tag="PKI23_04915"
     CDS             14722..15039
                     /locus_tag="PKI23_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNN36314.1"
                     /translation="MLNAADRKQYRTLGHGLKPVVTIANHGLSEGVLKELERALDDHE
                     LIKIKVISGDRHARKSLIDALIGATGCTLVQQVGQIALLLRRNPKANPALSNLLRPAA
                     RNR"
     gene            15405..16118
                     /locus_tag="PKI23_04920"
     CDS             15405..16118
                     /locus_tag="PKI23_04920"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36315.1"
                     /translation="MKKRNLLTIATTAAVSMAGLTAMNSASAELAANVGLSTIYLWRG
                     QDVSGGSPQFAGDLTFKDASGLYAGAWISSEANQAVLDTTVSPPSLTQDGGQEYDLYA
                     GWAATYAGIGIDASLWNYNYPSDATYDSFGDLSEFVLGLSYGPASFKWYKNIANTAGS
                     NDYQYFTLGLTIDKFSALVGTTDDSANSAGEYTHLDLTYSYNDRLSFTASTVVDKPDD
                     GSLDGDTLLVIAYKLPISL"
     gene            16339..17739
                     /gene="amt"
                     /locus_tag="PKI23_04925"
     CDS             16339..17739
                     /gene="amt"
                     /locus_tag="PKI23_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNN36316.1"
                     /translation="MAAGLAVSGFADEAPAAAAAAAVPVPNKGDVSWMLVSTLLVLMM
                     SVPALALFYGGMVRAKNMLSVLMQVFVVFSLITVLWCIYGYSLAFTEGNAFFGSFGRL
                     FLNGLFDSSTGTFAMAATFSKATVIPEMLFAAFQATFAAITCCLVVGAFAERTRFSAV
                     LLFMVLWFTFSYIPVAHMVWFWMGPDAYSDPSVVDAMNAKAGFIWAMGALDFAGGTVV
                     HINAGVAGLVGAYMVGKRTGYGREAMKPHNLTMTMIGAALLWTGWFGFNAGSALEANN
                     SAVLAFMNTFLATACAVLSWTFAEWLTKGKPSMLGAASGAVAGLVAITPAAGNVGIPG
                     AFAIGLIAGVVCLWGVNGLKRMLNVDDSLDVFGVHALGGILGALLTGVFNSPALGGPG
                     FVTDWVTAQTGFSSIGAQLLVQAKGVGVTVIWSAVVAFIAYKISDLMIGLRVPVDVER
                     EGLDVNEHGETAYHMS"
     gene            17815..18153
                     /gene="glnK"
                     /locus_tag="PKI23_04930"
     CDS             17815..18153
                     /gene="glnK"
                     /locus_tag="PKI23_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNN36317.1"
                     /translation="MKMVTAIIKPFKLDDVREALSDIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVAITDELLDQALEVIAKAANTGKIGDGKIFVVNLEQALRI
                     RTGETGKDAL"
     gene            18365..18826
                     /locus_tag="PKI23_04935"
     CDS             18365..18826
                     /locus_tag="PKI23_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNN36318.1"
                     /translation="MSRFIIAIIALLFAMQLQAADRVYRWSDAAGRPHFGDHPPADAP
                     SERMQLTSARFAAPPGEARTPQQEIVRLLRAMEADHAADAARLARQRQEAAVRAGRCQ
                     QAEQQLALYRLGMPVFKEGEQGERLFLNESERSAAFSAWRGAAERDCDSAS"
     gene            complement(18828..20669)
                     /locus_tag="PKI23_04940"
     CDS             complement(18828..20669)
                     /locus_tag="PKI23_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNN36319.1"
                     /translation="MRGMRPPIDEAPELESQSSRSDWATLRTLFPYLWRYRQRVILAL
                     LCLVLAKVATVSIPLLLKQIVDGMTAKPGIAVMVPLGLILAYGAVRLASTAFVQVRNA
                     IFAKVEQGAIRQVALEMFNHLHQLSLRFHLDRQTGGVSRDIARGTRGIGSLLSFLLFS
                     IIPTLLEIALTTAIFMTLYDWRFVSTTLVTVVIYVVFTIVVTNWRTQIRRTMNELDSR
                     ANVASIDSLLNYETVKYFGNETFEAQRYDAAMQRWEQAAIKNEKSMGVLNSGQSAIIA
                     VGVTLLVWLAADGVAQGSMTLGDLVAVNAFLIQLYTPLNALGMAYREIRQALTDMEKM
                     FALLREEAEVGDAAEARPLATTAARVDFDAVDFGYDPRRPILRKVTFSIPAGQRLAVV
                     GSSGAGKSTLVRLLFRFYDVSGGAVRIDGVDIRQLTQQSLRAHIGIVPQDTVLFNDTI
                     YYNIAYGRPTASREEVIAAARSAQIHHFIEQLPDGWQTLVGERGLKLSGGEKQRVAIA
                     RTILKNPPILIFDEATSALDSPTERSIQSALAEIARNRTTLIIAHRLSTVVDADEILV
                     LEQGEVVERGSHAQLLAQGGVYARMWLLQQHETAAELRHGGALAETG"
     gene            complement(20682..21053)
                     /locus_tag="PKI23_04945"
     CDS             complement(20682..21053)
                     /locus_tag="PKI23_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HNN36320.1"
                     /translation="MNANETLIRQFITAWSRLDAKELASYFTEDGCYHNMPTQPVCGR
                     ENVEKLIAGFSSSWKSTEWEVLNLISSGNLVMAERVDRAQTSQGDVALPCVGVFEIEA
                     GKIKVWRDYFDMATYANQLKG"
     gene            complement(21040..21867)
                     /locus_tag="PKI23_04950"
     CDS             complement(21040..21867)
                     /locus_tag="PKI23_04950"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNN36321.1"
                     /translation="MQTAPDHSRQRLLADDEPAAYQVCCPAGASPFFLTADHASHRLP
                     RVLGSLGLCDSDRQRHIGWDIGVAGLAKLLSAQLDAWLILQNYSRLVIDCNRPLDSPQ
                     SIVTLSEETPIPGNVALSPAEIAARQNEIFIPYHDRIRAELDLRQRQRRSTILVALHS
                     FTPCYLGVQRPWQIGLLYHRDPRLGRRLIELLRQEPGLTVGDNQPYFISDGSDYGIPV
                     HGEQRGIVHVEIEIRQDLLADAAGQAHWAALLARLLTQLQPEFDHPSSSKESANERQ"
     gene            complement(21931..23748)
                     /locus_tag="PKI23_04955"
     CDS             complement(21931..23748)
                     /locus_tag="PKI23_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN36322.1"
                     /translation="MDNHHLNLYCKFSAVAKAVPNAECMVQGRRRFSYAEVDRRTDAL
                     ANLLLAHGIQRGDRVSVMERNSPEHMECYLAAGKIAAIPCNVNYKYKGDELRYLLRDS
                     GSHTLFIHADFLPVLDEIRHEIDTLKLVVVIGEGAETSANQLGYEAALAEQLGQPLHF
                     DWRGPDNDDIQWLLYTGGTTGYPKGVMWDRQFWEYGHLLNTAGFAALLPKMKDAPREF
                     FAPASGERTTIVHRFLQSGLNRWLLGQQWVQDFLLKRITATALRESAGSLDELATAAR
                     KSIAAGKQARSMIACPLMHGTGYLSAIMTLTKGGALLFAESKNFSPKEIWEIIERERA
                     TSLSIVGDTFAAPLAAELEAKRYDTSALRSIVSSGVIFSAIHKRGLLKFNPQLVIMDS
                     LGASEGLSRAEPTLASDSGEAVRSMNFQLAPHMKVFDEQDNEVVPGSDTIGQLAVSGA
                     IPRGYWGDPEKTAKTFRVIRGVRYSIFGDFCKLREDGTLILLGRGTHCINTGGEKVYP
                     EEVEKVLKDLPEVEDCCVLGVPDERWGQAINAMVSFKPGQQLSEQRLIEHCEQKLSNY
                     KKPKKVWIVGEIPRRENGKMDYPQVKLLLSELTGVKLTA"
BASE COUNT         4150 a         7930 c         7337 g         4588 t
ORIGIN      
        1 gtgccggcca ccttgaaatc catgtcgccg aggtggtcct cgtcgccgag gatgtcggtc
       61 agcacggcaa agccgttctc ttccttgatc agccccatgg cgatcccggc caccggtgcg
      121 gagatcggca ccccggcatc catcagcgcc agcgaggcgc cgcagaccgt ggccatcgag
      181 ctggagccgt tggattcggt gatctccgac accacacgga tggtgtaggg gaactcctcg
      241 agagtcggca acaccacctc gataccgcgc cgcgccagcc ggccatggcc gatctcgcgc
      301 cgtttcgggc tgcccatcat ccccacctca ccgacgctgt agggcgggaa gttgtaatgc
      361 agcatgaagt tgtcgcggta gctgccctcg agggcgtcga tggtcgcgcc atcgcggatc
      421 gaaccaagcg tcgccaccac cagcgcctgg gtctcgccac gggtgaacag ggatgaaccg
      481 tgggtcttcg gcagcacacc ggtctcgacg aacagcggcc gcacggtgtg gttgtcacgg
      541 ccatcgatgc gcggttcgcc gcgcacgatc gaggcgcgga cgatcttctt ctccagtttg
      601 gcgaacagac cattcagttc ggccgccgtc gccggcgcga actgctcact gacgatcagc
      661 gcctcgatgg cccgctcgcg cagttgaccg agtcggtcgc ggcgctggct cttgtcggtg
      721 atgcgatagg cggccgccac ctcgtcggcg aaggcggcct gcagtgcggc gtagagcggt
      781 tcgttgatgg cgggcggctg ccactcccag cgcggcttgc ccgcctcgcg caccagctct
      841 tcgatcgcca tgatcaccac ctgcatctcc tggtgggcgt agagcaccgc gcccagcatc
      901 aggtcttccg gcagctcctt ggcctccgat tcgaccatca gcacggcatc cttggtgccg
      961 gccaccacca tgtcgagcgc cgatttttcc agctcggcat aggaggggtt gagcaggtag
     1021 cccgcctgcg gatcgaaacc gacccgggcc gcgccgatcg ggccatcgaa ggggatgccg
     1081 gagagcgcaa ccgccgccga agcgccgatc atcgccgcga tgtccgggtc attgaccttg
     1141 tcgaccgaca tcacggtgca gaccacctgc acctcctgca tgaagccctt gggaaagagc
     1201 ggccgcagcg gacggtcgat cagccgtgag gtcagtgtct ccttctcgct cggccgcgcc
     1261 tcgcgcttga agaagccacc cggaatcttg ccggccgcgt aggttttttc ctgatagttc
     1321 acggtgagcg gaaagaagtc ccggttcggc acgacctttc gatcaccgac caccgtgacc
     1381 agcacggtgg tctcatccac gctgaccagt acggcggcgg tggcctgccg ggcaattctg
     1441 ccggtttcga gcgtgacggt atgttgacca tacctgaagc tttttctgat tggattcacc
     1501 gaatcattcc ttaatggatt gcaagagccg gagctgggcg tgcatcgatc agcgacgcag
     1561 gccaagtcgg gcgatcaatt gggtgtagcg cgcgttgtcg gtgcgcttca ggtagtcgag
     1621 cagcttgcgg cgctggttga ccatgcgaat cagaccacgg cgggagtggt ggtccttctt
     1681 gttctgttcg aaatggggtt gcagcttctg gatgttggcg gtcagcaatg caacctgcac
     1741 ctcgacggaa ccggtgtcgc cctcactgcg ctggtactgc ttgaccactg ctgccttttc
     1801 ttctgcactc atcgacatct catgactcct gattgtggaa tatgcctgtt gatggactcg
     1861 gatccatcac tgcttggccg aacaccgcgc atatctgcag ccttcggccc cattgtggac
     1921 agcggtcagt ggctcgcgac cagtcgtcgc ggtgccagcc ggccatcatc cagaatttca
     1981 cccacgccga tgaagctgcc cgcaccatcg acgatgcgca cccagccact gctcggcagt
     2041 tgcggtgcca ttatcggctg cccctgcctg aagtaataga gcgtggtctc gttcagcttc
     2101 acctgcggga aatcactgac gctgctgctg gccggcagca gcaatgcatc cagcgccggg
     2161 taacccccct gttcgcgcag ttccgccagc gcatcgaggg tcaccgtctg cgaaatctcg
     2221 tagggaccgg cctcgatccg ccgcaactgc accacatggg cgccgctgcc cagggccttg
     2281 ccgagatcct cggccagcgt tcggatgtag gtccccttgc tgcaccgcac gtcgagttcg
     2341 atctcatcct cgcccagccg cagcagttcg agcgcatgga tggtcacccg ccgtggcggc
     2401 cgttcgacct cgatgccggc acgggccagc tcgtagagtg gcttgccctg atgtttgagc
     2461 gccgagtaca tcgacggaat ctgctcgatc tctccgcgaa agtgcgacag ggccgacagc
     2521 acgcgctcct gcgtcagctg cagcggtgcg aaggtctcga tcacctcgcc atcgacatcg
     2581 ccggtggtgg tgcgcgcccc cagcttgagg gtgacctggt aacgcttgtc ggcattgagc
     2641 agatagtgcg agaacttggt cgcctcgccg aagcagagcg gcagcacgcc ggtggccagc
     2701 ggatcgaggc tgccggtgtg tccggccttg gccacgccgc agatcgcctt ggcccgctgc
     2761 agcgcctggt tcgaggtgat gccggcgggt ttgtcgagca gcaggatgcc atcgacctgc
     2821 cggccacgtg ccttgcgctg tcgtcccact gctctgttca ctccttcgcc ccatgcgtgc
     2881 ggtcttgtgc gatggcctgg tcgatcagcc gcgacaggtg cgcgccccgc tcgctgctct
     2941 cgtcataaaa gaagtgcaac tccggcgtcg tgcgcatctg gctgactttg gagagccggc
     3001 cacgcagaaa accggccgcg ccacgcagcg cctgcagaat ctcctgccgt gcctgcgggc
     3061 tgggctggtc gaccacggtc acatggacgt tggcatgggc cagatcgcgg ctgacctcga
     3121 catcggtgac cgtcacgaaa cgggcacgcg ggtcgcgcat ctcgttctgg atctgcagcg
     3181 ccaactcccg cttgatgaag tcggccaccc gatcggtgcg agaaaactca cgcggcatgg
     3241 tccagcctca ctcgccccgg ccgttacagc gtgggtgcga tttgatgttt ttcatagact
     3301 tcgatcagat cacccgcccg cacgtcgttg tagttcttca ccccgatgcc gcactcggtg
     3361 ccactgcgca cctcgctggc atcctccttg aaccgacgca acgactccag ttcgccttcg
     3421 tagatcacca cccggtcgcg caacacccga atcggtttgt tgcggtggac caccccctcg
     3481 atcaccatgc agccggcaat ctggccgaat ttgggcgagt ggaagacttc gcgcacctcg
     3541 gccacgccga ggatctgctc gcggaaccgc ggtgccagca tgccggccag tgcctgacgc
     3601 acatcatcga gcaggtcata gatcacactg tagtagcgca ggtcgacgcc gccctcctcg
     3661 atgatcttgc gtgccttggc atcggcccgg acattgaaac cgaacagcac cgccttcgag
     3721 gccagcgcca gcgtggcatc ggtctcgctg atgccgccga caccaccgct gatcaccctg
     3781 acctgtacct cgtcggtgcc gatgtcggcc agggcgctct gaatcgcctc cagcgaaccg
     3841 cgcacgtcgg ccttcagcac cacgttgagc gtcttcacct cgccggcctg caggctttcg
     3901 aacatctcct ccatgcgcgg catggccggc tggcgactga gcttcacttc gcggtacttg
     3961 ccctggcgga acagtgccac ttcccgtgcc tttttctcgt cgctgaccac cacgaagctc
     4021 tcgccggcat cgggcgtgcc atcgaagccg agcacctcca ccggcagcga aggacccgcc
     4081 gcctcggtcg gtcgaccggc ttcgtccatc atcgcgcgca cccggccgta gtaggggccg
     4141 gccagcacca tgtcgccctt gcgcagggtg ccgttctgca ccagcatggt gaccaccgaa
     4201 ccgcggccct tgtccagccg ggattcgacc accacaccgc gacccggcgc gctcggcacc
     4261 gcagtcagtt cgaggatctc ggactgcagc agcagtgctt cgagcagctc atcgatgcct
     4321 tgtccggtgt gcgccgacac ctgcacgaac tgggtgtcgc cgccccactc ctccggaatg
     4381 acgccatggc gcaccagctc attctgcacc cgctccggat cggcggtggc cttgtcgacc
     4441 ttggtgatgg ccaccacgat cggcacctcg gcagcgcggg catgctgaat cgcctcttcg
     4501 gtctgcggca tcacaccatc gtccgccgcc accaccagca cgacgatatc ggtcagcttg
     4561 gcgccacgcg cccgcatcgc ggtaaaggca gcatggcccg gcgtatcgag aaaggtgacc
     4621 atgccgcgtg cggtctcgac gtggtaggca ccgatgtgct gggtgatgcc gcccgcttcg
     4681 ccgctggcca cccgtgtctt gcggatgtgg tcgagcagcg aggtcttgcc atggtcgaca
     4741 tggcccatca ccgtcaccac cggcgcacgg gtgactgctt caccttcgat cgagagtgac
     4801 gccgtcaact catcctcgag ggcgctgtcg ctgagcagct tcaccttgtg acccagctct
     4861 tcggccacaa tcgccgcgac atcctgatcg atcacctggt tgatggtggc catcgtgccc
     4921 atcttcatca tcaccttgat cagctcgcca gccttgatcg acatcttttg cgccagatcg
     4981 gccacggtga tggtgggtgg aatccccacc tcacgcgtca gggtctcggt gggtttggcg
     5041 aaggcgtgct gtgcaaacct gccccggcca tttttgccgg ggatcttctt gccgccacgc
     5101 cgaaaaccgg cgcggtcgtc ttcatcgaaa ctgagcgtgc cacgcgcacc cttgatctgt
     5161 gccgctggct tctctttctc cttgaccttg ctgcgtggac gcgcgcgctc atccttgacc
     5221 tcgaccggct tggtcttggc acgcgcggcg gcggcttctg cgtcggtctt gattggctgg
     5281 gcaggctgtt ccggcttgac ccccgcggcg tcggtctcgg tcgtggtcga cgcggcgcgc
     5341 tccgcttcac gggcccgcag cgcttcggcc tctttgcgtg cctcctcctc gcgctcacgc
     5401 tgccgagact cttcgatcag ccgcaaacgc tcctcttcct gcttcttgcg ctgcacctcc
     5461 tcggcgattc gctcgcgggc cgagcgctct tcggcaggcg ccggaggcac gacttcatgc
     5521 tgcgccacgg gtggtggagc ggacggaacg ggctgcgtct cttctgccgc atcgcgccgg
     5581 gcatagacct tcttcttgcg cacctcgaca ttgatggtct tgccccgccc ctgtccggtc
     5641 gccagcttga gcgtgctgac ctccttgcgc tgcagggtga tctttctggt ctcgccaccg
     5701 ctctcgccat ggctgcggcg caggtggctc aagagcttct gcttctcctc gtcggagacc
     5761 agatcatcga cgccggtttg tgacagaccc gcctcatgca tctgtttgag cagacgatca
     5821 actggtgcgc cgaccacttc ggcaagttgc ctgacggtca catctgccat gggtaccccc
     5881 ctctctcgac cgatcccaga atcgtctgat ggttattcat tcgtcgcaaa ccagtgcgcc
     5941 cgcgctgcca gaatcagtcg tccagcccgc tcttcatcca tctcttccat ctcgaggagg
     6001 tcatcgaccg actgctcggc cagatcctcg cgggtgacga tgccacgccg tgccagcagt
     6061 gccgccagtg acggatccat gccctctact tcgagaagat cctgcgcggg ctcgacctcg
     6121 tccagcttct cttcgttggc gatggcctgg gtcagcagca catccttggc ccggttgcgc
     6181 agctcttcgg cgatctcttc gtcaaaaccc tcgatggaga gcagctcatc gagcggcaca
     6241 taggcaatct cctccagcgt ggagaatccg gcctcgatca gcacggcggc cacctcttcg
     6301 tcgacatcca gcagcgtgac gaatttctgc agcaggccga ccgactcctg ctcctgcttt
     6361 tcgatggcgg cctgctcgct catgacgttg atttcccagc cagtcagctc ggaagccagg
     6421 cggatgttct gcccgccccg gccgatggcc tgcgccaggt tctcctcggc cacggcgatc
     6481 tccatggtgt gggcatcttc atccatgacg atcgacacca cttcggccgg tgccatcgca
     6541 ttgatgacga actgcgcctg gttgtcatcc cacagcacga tgtcgattcg ctcgccaccc
     6601 agttcgttgg agaccgcctg cacccgcgaa ccgcgcatgc cgacgcaggc gccgaccggg
     6661 tcgatccggc ggtcgttggt cttgaccgcg atcttggcac gggcacccgg gtcgcgggcc
     6721 gcccccttga tctcgatgat ctgctcggag atctcgggca cctccagccg gaacagctcg
     6781 atcagcatgc gcggatcggt gcggctcaga atcagttgcg gtccgcgcag ctccggtttc
     6841 acgtcggtca gcacgctgcg gacccgatca cccatgcgga agctttcacg cgggatggcc
     6901 tgatcacggc tcagcagcgc ctcggcgttg ttgcccagat cgacgatgat gtggtcgcgg
     6961 gtcaccttct tgacctgacc attgaccagt tggccgacgc gatgcaggta ctgcgccgag
     7021 atctgcccgc gctcggcctc gcgcaccttc tgcacgatca cctgcttggc cgcctgggca
     7081 gcaatccgcc cgaacagggt gttttcgacc tgctcttccc agacatcgcc cggctgcagg
     7141 gcagggctct tttcgtgggc ctcctgcagc gtcagctcgg tgcccagttc gggaacctgg
     7201 tcatcagcca ccacctgcca gcgacgaaaa gtctggtagt caccggtttt ctgatcgatc
     7261 acgacccgga tatcgctctc ttcatcctcg tagcgctttt tggtcgcggc ggccagcgcc
     7321 agctcgagcg cttcaaaaat gaggtgcggc ggtaccgctt tttcatggga taccgcatcc
     7381 accaccagca agatctcttt gttcattcga ctgtctcgct tcgctcaatc aaactcaggg
     7441 actatatggg ccttgtcgat ggcgcagagc ggcagaagat attcgtgctc tcccatcatc
     7501 agcaccacgt cttccccctc gatgccggtc aatctcccgt cgaagttgcg ccggccctca
     7561 aacagcgtcc gcagccggat cttcacccga ctgccgatga atcggcggta ctgctccagc
     7621 gtgtagagag ggcggtcgag gccgggcgag gagatctcga gatggtattg cccatcgatc
     7681 atctcggaga catccagcag tgcgcccacc tgccggctga cccgctcgca atcctcgatg
     7741 gtgacgcccc cgggctggcg atcgatgtag attctcaatc tggcactctt gctgtgcaca
     7801 ccatcctcga ttccccacag ctcgcagccc agcgcctgca ctgccggttc gatcaattgc
     7861 cacagttgct gcgtcttggg gttcatctgc gtgacaggac cgcactccat accgtgtagc
     7921 tctgttgagc cgaattcaat caaattttca cgaaaataaa aaatgggcca taggcccact
     7981 tgcgaatctc ctttgaccac accccggctg ccatgcacca gggttacaga ctcactgatc
     8041 aaccatcaat aaaaagcccc atgcggggcc tttttgatgg tagcgggggc tggatttgaa
     8101 ccaacgacct tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcatcagta
     8161 agggacatta ttctatctgc tcaaacagca ttgtcaacca gcgattggtg ccgaagagag
     8221 gacttgaacc tccacggggt tacccccact accccctcaa gatagcgtgt ctaccagttc
     8281 caccacttcg gcgaagggac ggcgagtata ccctactgct tcggccccgt ctctaccttt
     8341 ggcagctcct cggactgacc gccggccggt actggcgcag catcggacgg cttgacatca
     8401 gcagagtcgg tggcgctcct gtccggcagc agcccttggg cggggatctc gccgccttcg
     8461 gtcgcgctcg gcaggccctg ctcgatcgcc ggcaagccat gcagcgggtt ggcttcgccg
     8521 tcggccctgt tcttggcaat gacggccagg ctgatgctgg tggcaaagaa ggccatcgcc
     8581 agcccggtgg tgatgcgggt cagcaagctg ccgccgccgg tgctgccaaa gaccgtctgc
     8641 gatgcgccgg caccgaacga agcgcccatc gaggcgccct tgccctgctg gatcagcacc
     8701 agcgccacca gtgctacgcc caacagtaca tgaatgatca gaatgattgt ttccatcacc
     8761 gcttcgccgt agtacaaatc gccaaaaact gttctgcatc gagcgatgcc ccgccgacca
     8821 gaacgccatc gatgtcgggc tgactcagca gcagcgcgct gttgtcgggc ttgacgctgc
     8881 cgccatagag aagcggtacc gcccaccccc gagctgtcca cgccagcaac tgctgccgaa
     8941 tcgacccgtg cacctgctgc gcctcgtcag ccgaggcgga acggccggtg ccgatggccc
     9001 agactggctc ataagcgacc acgatgcgag ctgcatcttc gctgtcgagc cgctccagca
     9061 cggccttgag ctgccgctcc accacgctca aggtcaggcc cgcgtcacgc tccgtcacgg
     9121 tttcgccgac acagacgatg ggcttcagcc catgccgcag tgcggccatg ctcttgcgcg
     9181 ccaccagctc gtcgctctct gcgtacagct ggcgccgctc ggagtggccg acgatggcat
     9241 agcgacagcc aaactccctg accatcggcg ccgatatctc gccggtgaag gcgccctgcg
     9301 cctcttcatg gatgttctgc acaccgagtc gaagggtgct gttcgccagc aactgctcga
     9361 ggcgccacag gtagagggcc ggggggcaga gcacgatctc ggtaccgctg tcagggacga
     9421 ccccctcgac aatccggccc accaacgcat cgacgctggc gcgggagcca tgcatcttcc
     9481 aattcgccac cagcagcacc gggcgcatca gccgctcccc acgccgtcag ggcgggcgat
     9541 tctatcgccg tgcaatcgtg catggcaagc aacctgccat ctgttaacca ttggaacctc
     9601 cgaccaaggc cgccagctcc tgcgccaact ggctgatcat cgcggcatct tcgccctcga
     9661 ccatcacccg gatcagcggt tcggtgccgg aggggcgcag cagcagccgg ccgcgcccgg
     9721 ccagacgctg catgatctga cgctcggcag cgcgaatcgg ctccgaggca cagagcgccc
     9781 gtcggtccac catggcgtcg acctggacat tgatcatcac ctgcggcagc ttgcacatgc
     9841 cctgcttgat ctcatgcagc gtgcgatcgg agctgatcat cgccgccagc acctgcagcg
     9901 ccgagacgat gccatcgccc gtggtggtcg ccgccagaca gaggatgtga ccggagctct
     9961 cgccgccgag ttgccagccg cgctccccga gtgcctgcgt cacatagcgg tcaccgacat
    10021 cggtgcgcgc gaatggaatc ctgagttcct ccagcgccag ctccagcccc aggttgctca
    10081 ttttggtgcc gacgacaccg ccgatcaggc gacccgaacg ggcctgatcg gcggcgatga
    10141 tgtagagcag ttcatcgcca tcgacgatct cgccggtgtg gtcgaccatg atgacccggt
    10201 cgccgtcgcc atcgaaggca atgccgagat cggccccctc gctcaggacc cgctgtcgca
    10261 atgcctcagg atgggtcgag ccgcagccct ggttgatgtt gagtccatcg ggcgaggtgc
    10321 ccagctcgat gacccgggcg cccagctcac tgaagacttc ggtcgcgaca tggtaggtgg
    10381 cgccatgggc acaatcgacc accagcgtca gccggtcgaa atcgagcgcc gacggaacgg
    10441 tgctcttgca gaactccaca tagcggccag ccgcgtcact gatgcgaaag gccttgccca
    10501 actggtccgg cgcgactgtg accatcggct catcgatctg ctgttcgatg accagttcga
    10561 cctcatcggg cagcttggtg ccctgacggg agaaaaattt gatgccattg tcgtagtagg
    10621 ggttgtggga ggcgctgatc acgacgccgg cgcgcgcctt gaaggtgcgg gtcaggtagg
    10681 cgatggccgg ggtcggcatc ggcccgagca gcccgatgtc gacgcccgcc gccgacagtc
    10741 cggcttcaag ggcggattcg aacatgtagc cggagatgcg ggtgtccttg ccgatcagca
    10801 ccttggcgtc gggatgctgc cgggcgaaga cccggcccac cgcccagccg agcttcagca
    10861 cgaactccgc cgtgatcggc ggctggccga cccggccccg gatgccatcg gtaccgaaat
    10921 aacgtctgct catcatctgt ccacgttttc gttgaagatc gcccaggcca cgcgcagcgc
    10981 atcgacagcc ggcgcgacat catgcacccg gaacagtctg gcgccgcgct ccagcgcaac
    11041 cacctgggcc gccagagtgc catgcaggcg cgtctcgacc ggacggtcga ggatggcgcc
    11101 gatcatgctc ttgcgcgaca gcccgaccag cagcggcagg tcgagttcag ccagatggtc
    11161 gaggtgctgc aacaaggtca ggttgtgtgc gaccgttttg ccgaacccga accccggatc
    11221 gatgatcagc cgtgaacgcg gcagacccgc tgcctcacac tgcgcaaccc gctgcgccag
    11281 aaagatccgt acctcttcga ccacatcctg gtagccgggc gcctgctgca tggtttgcgg
    11341 atcgccctgc atgtgcatca gacagaccgg cagttccagt tcgaccgctg ccgccaaagc
    11401 gcccggctga cgcagcgccc gcacatcatt gatcaggcca gcgccgtgcc gggccgcctc
    11461 gcgcatcaat tccggacggc tggtatcgat cgacacgatg gtgtcgaact ccgccgtcag
    11521 ccgctcgacc accggaatga cccgatcgag ctcctgttgc agcgacacct cggtggcgcc
    11581 gggccgggtc gattcgccac ccacgtcgag cagttgtgcc ccctcggcca gcatctgctg
    11641 cgcggtcgcg agcagtggat cgagttgcac gccgctgctg ctgcggtaga gtcggccgcc
    11701 gtccgaaaag gagtccggcg tgatgttgag gatgcccatg acgcagggtg cagccagatc
    11761 gagccggcgc cctgcgcagt cgatcaccct tgatgtggcg cccatcgggc cgaaatcctc
    11821 ctggttccag aaacagtgaa accggtttcg ggccgtcgcg aaaccggttt cggtcagttc
    11881 gaccgcagac tcaatgttcg cctgccgtgc ctccgatcga gcccttgccc gatttctctt
    11941 cactggtggg ggatttcgcc ctgggcgggg cggcatcgcg gtcgctgtgc cagctctcgg
    12001 gttcccgtgg tggtcgacca ctcatgatgt cgtcgatctg ctcgaggttc agggtctcat
    12061 atttgatcag ggcctcggcc atggcatgca gcttgtcgat gttctcggtg aggagggtgc
    12121 gtgccctgtc gtagcagctg tcgatgatgc tgcgcacctc gtcgtcgatc ttgcgggcgg
    12181 tttccggcga gatcgaacgc tgctggctgc cgctgctgcg acccagaaag acttcggtgc
    12241 tgtcttcgtc atacatcaac ggacccagtt tctccgacag cccccagcgg gtcaccaggc
    12301 ttctggcgat gtcggtggca cgctggatgt cgttggacgc accagtggtg acgccatcgg
    12361 gaccgaggat cagctcttcg gccacccggc cgccgaacag gctgcaaatc tggctgatga
    12421 tcgagcgctt gctgtgactg tagcggtcct cttcgggcag aaacatggtg acgccgagcg
    12481 cccggccgcg cgggatgatg ctcaccttgt agaccgggtc gtgctccggc accagccggc
    12541 cgacgatcac atggccggct tcgtggtagg cggtgttgag cttctctttc tcggacatca
    12601 ccatcgattt gcgctcggcg cccatcatga tcttgtcctt ggccagctcg aactcatcca
    12661 tgtccacctt gcgcttgccg gcacgggcgg agaagagtgc ggcctcgttg accaggttgg
    12721 ccagatcggc gccggaaaaa cccggtgtgc cgcgggcgat gactgccggg tcgacatcgt
    12781 ctcccagcgg caccttgcgc atgtgcacct tcagaatctg ctcgcggccg cgaatgtcgg
    12841 gcagcccgac caccacctgc cggtcgaagc gaccgggacg cagcagcgcc gggtcgagca
    12901 catcgggccg gttggtggcg gcgatgacga tcacgccatc attgccctcg aagccatcca
    12961 tctcgaccag caactggttg agtgtctgtt cgcgctcgtc atggccaccg ccgagaccgg
    13021 cgccgcggtg gcggcccacg gcatcgatct cgtcgatgaa gatgatgcag ggggcgcgct
    13081 tcttggcctg ctcgaacatg tcacgcaccc tcgatgcgcc gacaccgacg aacatctcga
    13141 cgaaatcgga gccggagatg ctgaagaaag gcaccccggc ctcgccggcg atcgccttgg
    13201 ccaggagggt cttgccggtg ccgggctggc cgaccatcag cacgccacga ggaatgcggc
    13261 cgcccaggcg ctggaatttg cccggatcct tgaggaattc aaccagctcc ttgacatcct
    13321 ccttggcctc atcgacgccg gccacgtcgg caaaggtggt cttgatctgg tcttcgctca
    13381 tcaggcgggc gcggctcttg ccgaagctca tcgggccgcc acgaccgccc gcgccgccct
    13441 gcatctgccg catgaagaac atgaagaccg cgatgatgat caggatcggg aagctggcca
    13501 gcagcagctg catccagatg ctctgccgct ccggctgctg cccttcgacc tcgacaccat
    13561 gctcgagcaa ctcatccatc agcttgagat cgggcatgtt gggccgggtc acttcgaact
    13621 gaccgccgtt gttcttgcgc tgcccgacga tggtgtagcc atcacccttg atcgagatgc
    13681 gggcgacacg gtcctgattc agctcttcga tgaactgcga atagctcacc ttctgcatgt
    13741 cgggctggat gctgaagttg ttgaacaccg tcagcaacac ggccgcgatg atcaaccaga
    13801 ggatcaggtt cttggccata tcgttcaagg caatgtcctc gactggatgg ataatcgacc
    13861 ggctctgcga tggcacctgc caacccggag cccgcctgct tcattcatga ctgacggacc
    13921 actgcacgca ccacgcgaac agctttggat ttcattataa cgacagcctc caccttagcc
    13981 agaaacaggg cgctggccgc gaaagccggt tccaaccaga tagacctccg gcgagcgcgg
    14041 ccgggaggcc ttgggcttga cgcagcgcac ctgctcaaag gtggttcggc aattgccgag
    14101 gtaaccgtca aagccctcgc cctgaaacag cttggcgatg aatgccccgc ccggggtgag
    14161 cgcctgctgg gcgaaatcga gcgccagctc ggccagctcc atggccctgg gctgatccac
    14221 cgcccggttg ccgctgctgt tgggcgccat gtcggagagc accagatcga ccggctgccc
    14281 gcccagcacc cttctgagct gatcgagggc ggccgcttcg gtgaagtcgc cctcgatgaa
    14341 ctcgaccccg ccaatcggct ccatcggcaa ccgatcgatc gcaatcaccc ggccctcctc
    14401 tcccgtcatc tgcatcgcca cctggctcca gcctcccggc gcagcaccca gatcgatgac
    14461 cgtcatgccg cgacgcagca gacggtactt ctcgaccagc tcaagcagct tgaaactggc
    14521 gcgcgagcgg tagccgagct tttgcgcctg cttgacgtaa gggtcgtcga aatgttcccg
    14581 cagccattgg ccactggatc tgctgcgtgc cacaccgggc tcctggtcaa aggctgtcag
    14641 aggatatcga gatggttaca attgccagct tttcaacccc agccgatgac caatcactcc
    14701 accagggagc tacagaaaac cgtgttgaac gccgccgacc gaaaacagta tcgcacgctc
    14761 ggccatggat tgaagccggt cgtcaccatt gccaaccatg gtctgagcga gggcgtgctg
    14821 aaggagctgg aacgggcgct ggacgaccat gaactgatca agatcaaggt gatcagtggc
    14881 gatcgccatg cacgcaaatc gctgatcgac gcgttgatcg gcgccaccgg ttgtacgctg
    14941 gtgcagcagg tgggtcagat cgccctgctg ctgcgacgca accccaaagc caaccccgct
    15001 ctgtcgaacc tgctgcgtcc ggctgcacga aatcggtgac cccaggcgac cgatggttgc
    15061 gccagaatgg tgcgatcagg gctgcgacct gatcgagcat gtcgggtgca caattccacg
    15121 cactggttgt atacatcggc caaagcggca caccatcaaa acaacaatgc gcctgtttcg
    15181 cgcagagtgc gccaaaacag gtttggaaaa caggatcaat gcaccatgtt gaggcaatga
    15241 tcctttctcg atgccgggca tcgcggcaaa cctgagcggt tttgccccct ttcaatctgg
    15301 catgttatgt gaatggctcc agtggcgaac ccgccgatga agcgtgcttc gggccgcttc
    15361 tcgtcgtact tgcgcctaca cgcaccacag gagattgaat gatgatgaag aaacgcaacc
    15421 tgctgacgat cgcaaccact gctgcggtct ccatggctgg cctcaccgcg atgaacagcg
    15481 cgtcggccga actggccgcc aatgtcggtc tctccacgat ttacctctgg cgtggccagg
    15541 atgtcagcgg tggctcaccg cagtttgccg gtgacctgac cttcaaggat gccagcggcc
    15601 tctatgcggg tgcctggatc tccagtgagg cgaatcaggc ggttctggat acgactgtgt
    15661 caccgccctc tttgacccag gatggtggcc aggagtatga cctctatgcc ggctgggcgg
    15721 cgacctatgc cggcatcggc atcgatgcca gcctctggaa ctacaactac ccgtccgatg
    15781 ccacctatga ctcgtttggc gacctctccg agttcgtgct ggggctctcc tatggccccg
    15841 ccagcttcaa gtggtacaag aacatcgcca atacggcagg cagcaacgac tatcagtact
    15901 tcaccctggg tctgaccatc gacaagttca gcgccctggt cggcaccacg gacgacagcg
    15961 ccaacagcgc cggtgagtac acccacctgg atctgaccta cagctacaac gaccggctga
    16021 gcttcaccgc cagcacggtg gtcgacaagc ccgatgatgg ctcgctggat ggtgacacgc
    16081 tgctggtcat cgcctacaag ctgccgatct cgctgtaacc ggagtggtcg ctgctgagcc
    16141 ggttcaggcc ggttcagcag cggcatgaga agaaggacgg ggtcaccgca atgagtccgg
    16201 cgccccccaa agacgtcagg atcggatcca caagaaccca gacaggatac ggaggcactg
    16261 cgcgcctcgc actgtcgaac ctgaaccgtt tggaggattg aatcaatgag aaaaattgtc
    16321 tcctgtcttg ccctgctgat ggcggcaggc cttgccgttt ccggctttgc cgacgaggcc
    16381 ccggccgcgg cggcagcagc ggccgtgcca gtgcccaaca agggcgatgt cagctggatg
    16441 ctggtgtcga cgctgctggt gctgatgatg agcgtccccg cgctggcact gttttacggc
    16501 ggcatggtgc gcgccaagaa catgctgtcg gtactgatgc aggttttcgt ggtcttctcg
    16561 ctgatcaccg tgctctggtg catctacggc tacagtctgg ccttcaccga gggcaatgcc
    16621 ttcttcggca gctttggccg gctctttctc aatggtctgt tcgattcgtc aaccggcacc
    16681 ttcgcgatgg cggccacctt cagcaaggcg accgtcatcc cagagatgct gtttgccgcc
    16741 ttccaggcca ccttcgccgc catcacctgc tgtctggtgg tgggcgcctt tgccgagcgg
    16801 acccgcttct cggccgtgct gctgttcatg gtgctctggt tcaccttctc ctacattcca
    16861 gttgcgcaca tggtctggtt ctggatgggg cccgatgcct acagcgatcc gtcggtggtc
    16921 gatgccatga acgccaaggc cggcttcatc tgggcgatgg gtgcacttga tttcgccggc
    16981 ggcacggtgg tgcacatcaa cgcaggcgtg gccggactgg tcggtgccta tatggtcggc
    17041 aagcggaccg gctatggccg cgaggcgatg aagccgcaca acctgaccat gaccatgatc
    17101 ggcgcggcgc tgctgtggac cggctggttc ggcttcaatg ccggctcggc gctcgaagcc
    17161 aacaactccg ccgtactggc cttcatgaac accttcctgg ccacggcctg cgcggtgctc
    17221 tcctggacct tcgccgagtg gctcaccaag ggcaagccat cgatgctggg cgctgcctcg
    17281 ggcgcggtcg caggactggt ggccatcacc ccggccgccg ggaatgtcgg catccctggc
    17341 gccttcgcga tcggtctgat tgccggcgtg gtctgcctgt ggggcgtgaa tggcctgaaa
    17401 cggatgctga acgtcgatga ttcgctggat gtctttggcg ttcacgcgct tggcggtatt
    17461 ctgggcgcgt tgctgactgg cgtgttcaac tcgccggcct tgggtggacc cggcttcgtc
    17521 accgactggg tcacggcgca gaccggtttc agcagcatcg gtgcgcagct gctggttcag
    17581 gccaagggcg tgggcgtcac cgtcatctgg tcagccgtgg ttgccttcat cgcctacaag
    17641 atcagtgacc tgatgatcgg tctgcgggtg ccggtggatg tggagcgcga agggctcgac
    17701 gtcaacgaac atggcgagac cgcctatcat atgagctgac agagtgacgg ccgggcggta
    17761 cgctccgccc ggccggatca agaatggtta ccgcgaattc agaggattga ctgcatgaaa
    17821 atggttactg cgatcatcaa gcccttcaaa ctggacgatg tacgcgaagc gctttccgac
    17881 atcggcgttc agggaatcac cgtcaccgag gtcaagggtt tcggccggca aaagggccat
    17941 accgagctct accgtggcgc agaatatgtc gtcgactttc tgcccaaggt caagatcgag
    18001 gtggccatca ccgacgaact gctcgatcag gcgctcgagg tgattgccaa ggccgcgaac
    18061 accggcaaga tcggcgacgg caagatcttc gtcgtcaatc tggagcaggc cctgcggatc
    18121 cgcaccggcg aaaccggcaa ggatgcgctc tgagcgctga cgactgacag aggattcagg
    18181 caaccgttca gttcaccggg cttgcaggag cccggtcagg tttggcgagt gggagactta
    18241 aggggcgcac tgcgcccctc ttttttatgt gcgctgcttc acgaaggtga atgaagcatc
    18301 gtcatcgtga tcaggccgct gactatgctt cgggaaggtt ctccgcccac cccgtcccaa
    18361 gcagatgtca cgcttcatca ttgccatcat cgccctgttg ttcgccatgc agctccaggc
    18421 tgccgatcgg gtctaccgct ggagtgatgc cgcaggcaga ccccattttg gtgaccaccc
    18481 ccccgctgac gctccgtcag agcgcatgca gctcacctcc gcccgatttg ccgcaccacc
    18541 gggcgaggcc cggacaccgc agcaggagat cgtccgactg ctgcgggcca tggaggccga
    18601 ccacgccgct gacgccgccc gcctggcgcg gcaacggcag gaggctgcgg tgcgtgcagg
    18661 gcgctgccag caggccgagc agcagctcgc gctctaccgg ctcgggatgc cggttttcaa
    18721 agagggtgaa cagggtgaac ggctgtttct gaatgagagc gagcgctctg ccgcattcag
    18781 cgcctggcgt ggcgcggcgg agcgcgattg cgacagcgcg agctgactca gcctgtctcg
    18841 gccaaagcgc cgccatggcg cagttccgct gcggtttcgt gctgttgcag cagccacatc
    18901 cgcgcgtaga ccccgccctg cgccaacaac tgcgcgtggc tgccacgctc gacgacctca
    18961 ccctgttcca gcaccagaat ctcatcggcg tcgaccacgg tcgacaggcg atgggcaatg
    19021 atcagcgtgg tccggttgcg ggcgatttcg gccagcgcgc tctgaatcga ccgctcggtg
    19081 ggtgaatcga gcgccgaggt cgcctcgtcg aaaatcagga tcggcggatt cttcaaaatg
    19141 gtccgggcga tcgccacccg ctgcttttcg ccgccggaga gcttcagtcc gcgctcgccg
    19201 accagggtct gccagccatc gggcagctgt tcgatgaagt gatggatctg cgccgaacgt
    19261 gctgcggcga tcacctcctc ccggctggcc gtcgggcggc cataggcgat gttgtagtag
    19321 atggtgtcgt tgaacagcac ggtgtcctgc ggcacgatgc cgatgtgggc gcgcaggctc
    19381 tgctgggtca gttgccggat gtcgacgcca tcgatgcgca ccgcgccacc actgacgtca
    19441 taaaagcgga acagcagccg caccagcgtc gacttgcccg cccccgaact gccgaccacc
    19501 gccagccgct ggccggccgg aatcgaaaaa gtgaccttgc gcaggatcgg ccggcgcgga
    19561 tcgtagccga agtcgacagc gtcaaagtcg acccgcgctg ccgtggtggc cagtggccgg
    19621 gcctccgctg catcgcccac ctcggcctct tcgcgcagca gcgcaaacat cttctccatg
    19681 tcggtcaatg cctgacgaat ctcccggtag gccatgccga gcgcattcag tggcgtgtag
    19741 agctgaatca gaaacgcatt gaccgcaacc agatcgccga gggtcatgct gccctgggcc
    19801 acgccatcgg cggccagcca gaccagcagg gtgaccccga cggcaatgat cgccgactga
    19861 ccgctgttga gcacccccat cgatttttca ttcttgatcg ccgcctgctc ccaccgctgc
    19921 atcgccgcgt cgtagcgctg cgcttcaaag gtttcgttgc cgaaatattt gaccgtctca
    19981 tagttgagca gcgagtcgat cgaggcgaca ttggcccggg agtcgagctc gttcatcgtg
    20041 cgccgtatct gggtgcgcca gttggtgacg acgatggtga agaccacata gatgacgacg
    20101 gtgaccagcg tcgtcgacac gaaccgccag tcgtagaggg tcatgaagat cgcagtggtc
    20161 agggcgatct ccagcagggt ggggatgatg ctgaacagca ggaagctgag cagcgagccg
    20221 atgccgcgcg tcccgcgcgc gatgtcacgc gagacgccgc cggtctgccg atcgagatga
    20281 aagcgcaggc tcagctggtg caggtggttg aacatctcca gcgccacctg gcggatggcg
    20341 ccctgctcca ccttggcgaa gatggcgttg cgcacctgca cgaaggcggt cgaggccagc
    20401 cgcacggcac cgtaggccag gatcagtcca agtggcacca tcaccgcgat gcccggcttc
    20461 gccgtcatgc catcgacgat ctgcttgagc agcagcggaa tcgacacggt ggccaccttg
    20521 gccagcacca gacagagcag cgccagaatc acccgctgcc ggtagcgcca caggtagggg
    20581 aagagcgtgc gcagggtggc ccagtccgag cgtgacgact gactctccaa ctcgggcgct
    20641 tcatcgattg gagggcgcat gccgcgcatc ggcctgtctc ctcaaccctt gagctggttg
    20701 gcgtaggtgg ccatgtcgaa gtagtcgcgc cacaccttga tcttgccagc ctcgatctcg
    20761 aacacgccga cgcaggggag tgccacatca ccctggctgg tctgcgcgcg gtcgacccgc
    20821 tcggccatca ccagattgcc gctggaaatc aggttcagga cctcccattc ggtgctcttc
    20881 cagctgctgg aaaagccggc gatcagcttc tcgacattct cccggccgca caccggctgg
    20941 gtcggcatgt tgtgatagca gccatcctcg gtgaagtagc tggccagctc cttggcatcg
    21001 aggcggctcc aggcagtgat gaattggcga atcaaggtct cattggcgtt cattggcgct
    21061 ctccttggag gatgatgggt gatcgaattc gggttgaagc tgcgtcagca gccgggccag
    21121 cagcgctgcc cagtgcgcct ggccggcggc atcggccagc agatcctggc gaatctcgat
    21181 ctcgacatgg acgatgccgc gctgttcgcc atggaccggg atgccatagt cgctgccgtc
    21241 gctgatgaag tagggctggt tgtcaccgac cgtcagcccc ggctcctgcc gcagcagctc
    21301 gatcagccgc cggcccaggc gcgggtcgcg gtggtagagc aggccgatct gccacggtcg
    21361 ctgcacgccg aggtagcagg gggtgaagct gtgcagcgcc accaggatgg tgctgcgccg
    21421 ctgtcgctgg cgcagatcga gttcggcgcg gatgcggtca tggtagggga tgaagatctc
    21481 gttctggcgc gcggcgatct cggccggcga cagagcgaca ttgcccggga tcggcgtctc
    21541 ttcgctcagc gtgacgatcg actgcggact gtcgagcggc cggttgcagt cgatcaccag
    21601 ccgcgagtag ttctgcagga tcagccaggc atcgagctgc gcagagagca gcttggccag
    21661 gccggccacg ccgatgtccc agccgatgtg gcgttgccga tcactgtcgc acaggccgag
    21721 gctgccgagc acacgcggca gccggtggct ggcgtggtca gcggtcagaa agaagggtga
    21781 ggcgcccgcc gggcagcaga cctgatacgc ggcgggttca tcgtcggcga gcagtcgctg
    21841 gcggctgtga tcgggggcgg tttgcatcgg gccttgcaat catcacgtgg ctgccgcgcc
    21901 gacagtggcg cggcgcggca gggagctgga tcaggcagtg agcttgactc cggtcagttc
    21961 ggagagcagc agcttgacct gcggatagtc catcttgcca ttctcgcgac gcggaatctc
    22021 gccgacgatc cagaccttct tcggcttttt gtagttcgac agcttctgtt cgcaatgctc
    22081 gatcagccgc tgctcgctca actgctggcc cggcttgaac gagaccatcg cattgatcgc
    22141 ctgcccccag cgctcgtcgg gtacgcccag cacgcagcaa tcctccacct cgggcagatc
    22201 cttcagcacc ttctcgacct cttcggggta gaccttttcg ccgccggtgt tgatgcagtg
    22261 ggtaccgcgg ccgagcagga tcagcgtgcc atcctcgcgc agcttgcaga agtcgccgaa
    22321 gatcgaatag cgcacgccgc gaatgacacg gaaggtcttg gcggtcttct ccgggtcgcc
    22381 ccagtagccg cgcgggatgg cgccgctgac ggcgagctgg ccgatggtgt cgctgcccgg
    22441 caccacctcg ttgtcctgct cgtcgaacac cttcatgtgc ggtgccaact ggaagttcat
    22501 gctgcgcacc gcctcgccgc tgtccgaggc cagcgtcggt tcggcacggc tcagcccctc
    22561 ggaggcgccg agcgaatcca tgatcaccag ttgcgggttg aacttgagca ggccgcgctt
    22621 gtgaatcgcc gagaagatga cgccggacga aacgatgctg cgcagcgcgc tggtgtcgta
    22681 gcgcttggcc tccagctcgg ccgccagcgg tgcggcaaag gtgtcgccga cgatggagag
    22741 cgaggtggcc cgttcgcgct cgatgatctc ccagatctcc ttcgggctga agttcttgct
    22801 ctcggcgaac agcagtgcgc cgcccttggt cagggtcatg atcgccgaga gatagccggt
    22861 gccatgcatc aatggacagg cgatcatgct gcgggcctgc ttgccggcgg cgatgctctt
    22921 gcgggccgcg gtggccagct catcgagcga gccggcactc tcgcgcaggg cggtcgccgt
    22981 gattcgcttc agcagaaaat cctgcaccca ctgctgcccc agcagccagc ggttgaggcc
    23041 gctctgcaga aagcggtgaa cgatggtggt gcgctcgccg ctggccggag cgaaaaactc
    23101 tcgtggtgca tctttcatct ttggcagcag cgcggcgaag ccggccgtat tgagcagatg
    23161 gccatactcc cagaactgcc ggtcccacat cacccccttg gggtaaccgg tggtgccgcc
    23221 ggtgtagagc agccactgga tgtcgtcatt gtcggggccg cgccagtcga aatggagcgg
    23281 ctggccgagc tgctcggcca gcgctgcctc gtagccgagc tggttggccg aggtttcggc
    23341 accctcgccg atcaccacca ccagcttcag cgtgtcgatc tcgtggcgaa tctcgtccag
    23401 caccggcagg aagtcggcat ggatgaacag cgtgtgcgag cccgagtcgc gcagcagata
    23461 gcgcagctca tcacccttgt acttgtagtt gacgttgcag ggaatcgccg cgatcttgcc
    23521 ggccgccaga tagcactcca tgtgctccgg cgagttgcgt tccatgaccg acacccggtc
    23581 accgcgctga atgccgtgcg ccagcagcag attggccagc gcatcggtgc gccgatcgac
    23641 ctcggcatag ctgaaccggc gcctgccctg caccatgcac tcggcgttgg gcaccgcctt
    23701 ggccactgcc gaaaacttgc aatagaggtt gaggtggtgg ttgtccatga tgcggctcct
    23761 gctgagttcg ctgttgttat tgtcgtgatc gctccatctc gccggcgctc ggtgcgccag
    23821 cccgccgaag ctagccgatg gctgccatca agtaaagtgg ccgtcgtggt cttcgactgg
    23881 catcggcgcc cgccatctgc taaaaggcgg ctgcaacccc tccattcatt cattctcatc
    23941 ggtacggaag gtcaccatgc gccgcctgct cgccaccgct catgcgctgc tgctccttgt
    24001 actca
//
LOCUS       DAORCQ010000031        23010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1273_length_23010_cov_5.423699, whole genome shotgun sequence.
ACCESSION   DAORCQ010000031 DAORCQ010000000
VERSION     DAORCQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 23010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23010
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_23010_cov_5.423699"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="rodA"
                     /locus_tag="PKI23_04960"
     CDS             <1..1071
                     /gene="rodA"
                     /locus_tag="PKI23_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNN36323.1"
                     /translation="KPWLKRLHIDPLLLLLLLLLAGYGLVVLYSAGGGSLDAIERQGM
                     SLGLAGVLMLIAAQIDVAILRRWALVIHLGGTVLLIIVLLAGDDAKGATRWIDLQVLR
                     FQPAEIMKLAMPLAVCRYLGDHHLPPTLPTVAVSMALIAVPTLLIAKQPDLGSALLIA
                     SSGLYALFLAGIRWLWIALAGLAGVLLAPALWFLLRDYQRRRILTLIDPEADPLGSSW
                     NIIQSKIAIGSGGLHGKGWLAGTQSHLDFLPESHTDFIFAVLAEEFGLVGVLLLFGLY
                     LIIVARGLWIAAKAPDLFQRLLAGSITLTFSVYALVNVGMVSGVLPVVGVPLPLISQG
                     GTALVTLMLGFGVLMSIQTRTP"
     gene            1220..2125
                     /gene="mltB"
                     /locus_tag="PKI23_04965"
     CDS             1220..2125
                     /gene="mltB"
                     /locus_tag="PKI23_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNN36324.1"
                     /translation="MQQFITELVEHHNFSRPELEALFSQVQRQQRVIDLISKPAEKRL
                     EWFEYRRIFLDQERIRQGVQFWRAHAAELARAESQFGVEPPMVVAIIGIETRYGRNTG
                     TTPVLDALTTLAFDYPPRSKFFRQELLNYLLLAREEGKDPRSIKGSYAGAMGYGQFMP
                     SSYRNYAVDFDGDGKRDIWNNRSDAIGSVASYFNRHGWQHNAPVVMTALPSAENPALA
                     LNDTKKPTLSVAQLAAQGVRARQWVEPERKAVALRLLGDAGPEYWLAFDNFYVITRYN
                     QSQLYAMAAHQLSEAIRQHYIAGAD"
     gene            2126..3034
                     /locus_tag="PKI23_04970"
     CDS             2126..3034
                     /locus_tag="PKI23_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNN36325.1"
                     /translation="MQQNKAIQLISLLIFSTLLVACGSQPRIPPPPGTSRIDIPVTAP
                     AAPPASAEPYSPRGNRSPYQVFGQTYYVLPTAQGYQAEGTASWYGEQFHGLQTSNGEI
                     YNMYALTAAHKSLPIPCYARITHLGNGRQVVVRVNDRGPFHDDRLIDLSYGAALQLGF
                     ADVGLAQVRIEVINASATPLSPTAAVVASTPKPTRVSAENGVPAVIKRKPPGSTTAAR
                     SAAEAGAGLYLQAGAFSRREAAAAMVERLTDVVKHPISISTADTAKRESLFRVRIGPI
                     ASQDEAQQIQSLISKHGIASSFAFRQ"
     gene            3246..4409
                     /locus_tag="PKI23_04975"
     CDS             3246..4409
                     /locus_tag="PKI23_04975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNN36326.1"
                     /translation="MLSASTGAEQPVPTPAPVPVAVIPGPPALAASAYVMMDADSGRI
                     LAAQNENERMAPASLTKILTSYMVAEELQKGRIRETDEVNISIKAWQMGGSRMYVREG
                     TKVPLLDLLRGVVIQSGNDASVALAEHISGSEETFAEEMNRIAAKIGMKSSHFVNATG
                     WPSPDHYTTAHDLALLARALVKNHPEHYNLYSEKYFHYNNINQPNRNRLLWIDPSVDG
                     IKTGHTEEAGYCLVASAKRDNMRLVTVVLGTSSDQARTTESQKLLTYGFRYFETHKLY
                     NGGEALQRARVYAGAQNEVELGLAQDLHVTVPRGQYGSLAASLEIPGIIKAPLAAGQN
                     LGKIVLKLGETPLLEQPLTVIKPVEEGGWFKRLLDAVRLFFLQLFGGDGAGKS"
     gene            complement(4437..5240)
                     /locus_tag="PKI23_04980"
     CDS             complement(4437..5240)
                     /locus_tag="PKI23_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNN36327.1"
                     /translation="MATIPQLPGYAFHEQQFGDSALRFYRRGQGPGVIVMHEIPGITP
                     QVQAFADRVADAGFTVCLPWLFGVPGAPLSGGYLFRSMLGACVRREFTAFALGKSSPI
                     ITPLRALARALHQELGGPGVGALGMCFTGGFALGMMLEPAVLAPVLSQPSLPFPITER
                     RKSAIDIDPVSLSQVKNRLEQEQLSVLGLRFSHDPICTQARFDGLREQLGDHFEAIEI
                     DSGPGNPHGISGKSHSVLTNDLVDEAGHPTRVALDRVLAFFGERLRATD"
     gene            complement(5309..6472)
                     /locus_tag="PKI23_04985"
     CDS             complement(5309..6472)
                     /locus_tag="PKI23_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36328.1"
                     /translation="MGYMSEERLMIQETARQFSMKEVLPVANALDPIQGDIPLELRQK
                     MADMGYFGIRIKEEFGGLGLGCFEYCLIAEELARGWMSVASIIARSGGMMGLGNMPRE
                     KRIELTRKAARGEYLAAAALSEPNVGSDLSGVSCRAERRGDHWYITGNKYWCTFADGA
                     DYISVLARTSERDPTAPWKGLGRFIVEKPRGELPQGCQGSPIPKIGYFGWKTWELRFD
                     DCKVPVEYQMGNEDEAFTSTASGLEIARAHTAARAIGLARGALEDALKYAQQREQFGR
                     PIGEFQAIRFKLATMATEIEAARQLMYHVCDQIDRGGRCDQEASMAKYFATEMAERVT
                     SEALQIFGGAGYTKLHAVERYWRDARLTKIFEGTSEIQQRIISDRLLGKPRRG"
     gene            6585..8135
                     /locus_tag="PKI23_04990"
     CDS             6585..8135
                     /locus_tag="PKI23_04990"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNN36329.1"
                     /translation="MQATASIHYWIAPSTRSDEIDNPPLPNELHPVSQRARRGELKLY
                     DSFDWRLWFGGLALYRLDGCYHLLPRSGFRLDLARPDGSGYSRPINFAEQFREPELRQ
                     PVQRILAPRALIEIASFQCRERSVQVRNGDDKGVALLHCFELSHPLPDGRQARLEGLW
                     IAPCIGYQLELEMIEATLRAAGWQHEMRNPLELLLPAAQQPRPERTRPLTDMVGEEPA
                     DDLLRRLLLAQLRVVKEQEDGIIKQIDSEFLHDYRVALRKARSLLSLAKRVFPAAIQQ
                     RARHDFADLQRQSNQLRDLDVWLLQREHFQKMVPPRLQTGFEQLFAELQATRNQAQRR
                     LRRLLNSSAHQLRMQQWLYDLAQPGLWEEPAEVKRSARALIDRQILRRHRQLLKGAQR
                     FSQLDDEEVHDLRIEVKKLRYLLEIFSYFYSKEPCDWLLTHLRKVQGRLGDYNDLQVQ
                     QTHLQHFAETLIVGEKSTGEVLLAAGAIVAGLEREKQQARKRIAEPLHRLATPNGRAQ
                     LQQLLNEQ"
     gene            8140..8895
                     /locus_tag="PKI23_04995"
     CDS             8140..8895
                     /locus_tag="PKI23_04995"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN36330.1"
                     /translation="MKVIACYSIKGGVGKTATAVNLAHAAATSGYRTLLVDLDPQAAA
                     TFYFRIKAPEEKKVPRWFRTGQDLQQAIRGSDYPRLDLLPADLGYRDSEAMLAGMKRS
                     DKQLRKRLKEVADGYDMVLIDSPANIGLLSENIFRAADVVLVPVIPTTLSERTLRLLL
                     HFFAKQKLDVDRLLPFFSMVQPHKRLHADTMARLRAEFPQLLQSTIPFTVDIENMGVL
                     REPVLAYAPRRPAGEAYRALWLELLGRLIPRQE"
     gene            9020..9607
                     /locus_tag="PKI23_05000"
     CDS             9020..9607
                     /locus_tag="PKI23_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNN36331.1"
                     /translation="MAQSSSPVLSGERILRLNLAGQPIEWLTWQEAVSLYARDLVGWT
                     LGEVVCTLRGGISRLSQRRSRLELHSIIACRGKIHDPCQYEPPLTNRGLFRRDQNLCL
                     YCGKSLSDRLLTRDHLVPTSRGGRDEWTNVVTACRSCNQGKGNRLLHEARMELLALPY
                     RPNWAEYLALENSGRVRGDQMEFLRRQFSPHCRFA"
     gene            9604..10035
                     /locus_tag="PKI23_05005"
     CDS             9604..10035
                     /locus_tag="PKI23_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNN36332.1"
                     /translation="MINPDDLWVESFSKMDVEIVLKEKFLHVRASGHYSLDEMNAMSR
                     AMFAALQQHPRASCLIDCSQVTGSPTLNERFEWPRFTARLIEDLPAIQRQTRFAFVAS
                     EPFLDPGRFGETVAVNRGVNVVATDSLDEALRWLGVETGSA"
     gene            10032..10415
                     /locus_tag="PKI23_05010"
     CDS             10032..10415
                     /locus_tag="PKI23_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNN36333.1"
                     /translation="MKVEITPKEHYLHVRAVGEFSVAGMKAMSLAIIAALVEHSSSKC
                     LLDCRQVVGARTLAERFSWIEFAADESSKLPFASNRARIAYVGAANRLDPGRFGETVA
                     INRGLNVMVTDSLDEALRWLDLQAD"
     gene            complement(10462..11640)
                     /locus_tag="PKI23_05015"
     CDS             complement(10462..11640)
                     /locus_tag="PKI23_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNN36334.1"
                     /translation="MLIDFFMALKRSNIPVSLREYMDLLEAMNARLVYGDLDEFYYLS
                     RAIMVKDETHFDRFDVAFGRYFKGLETLEGIIAAFIPEEWLRKAFEESLTPEERAKIE
                     SLGGLEKLIEEFKKRLEEQKERHEGGNKWIGTGGTSPFGAQGYNPEGIRIGQDGNRNF
                     RAAKVWETRQFKDLDDTVELGTRDIKMALRRLRRFARSGAEVELDLDETIDATAKRGG
                     LLDLKFVPERHNAVKVLIFFDVGGSMDPYVRICEGLFSAVRTEFKHLEYFYFHNFIYE
                     SVWKSNTRRMHERTATWDLLHKFSSDYKVIFVGDAAMAPYEITNIGGSIEHNNEESGA
                     TWMQRVVETYERVIWLNPQAQDTWEYSNSVQMVNQLLDGRMFPLTLRGLDEGVSYLGK
                     "
     gene            complement(11644..12492)
                     /locus_tag="PKI23_05020"
     CDS             complement(11644..12492)
                     /locus_tag="PKI23_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNN36335.1"
                     /translation="MNFEGTQSYVATKDLQMAVNAAIRLQRPLLIKGEPGTGKTMLAE
                     EVAEALKLPIIKWHIKSTTKAQQGLYEYDAVSRLRDSQLGEARVHDISNYIVKGKLWE
                     AFDADQQVVLLIDEIDKADIEFPNDLLLELDRMEFYVYETQQRVVARRRPIVIITSNN
                     EKELPDAFLRRCFFHYISFPDRETMIQIVKVHYPEIKQELVKEALEIFFDVRTVPGLK
                     KKPSTSELIDWLKLLMADDMPEEVLKNKDAAKAIPPLYGALVKNEQDVHLLERLAFMH
                     RRQSRG"
     gene            12667..13722
                     /locus_tag="PKI23_05025"
     CDS             12667..13722
                     /locus_tag="PKI23_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNN36336.1"
                     /translation="MSSPIETDAVIVGAGPVGLFQVFELGLLDIRAHVIESLPQVGGQ
                     CSELYPDKPIYDIPALPICGAQELIDRLMEQIRPFSPTFHLGQEVNKVQQREDGRFDV
                     GTSAGTQFIAGNVIIAGGVGSFQPVQVEVPGIDRLLDHSLFYRVKDPARHHGKRLLVV
                     GGGDSALDWTLSLCDQAQSLTLIHRRNEFRAQPNSVNQMHELCAAGKMNFKVAVVADF
                     EADGTQLKRVALKSGQGEPEWIECDHILALLGLTPNLGPIGEWGLEIVRKQVVVDTEK
                     FQTNVPGIFAVGDINSYPGKKKLILSGFHETALAAFAIKERLQPGKKVHLQYTTTSPA
                     LQERLGVRGSDGETSTQ"
     gene            complement(13756..14661)
                     /gene="rimK"
                     /locus_tag="PKI23_05030"
     CDS             complement(13756..14661)
                     /gene="rimK"
                     /locus_tag="PKI23_05030"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HNN36337.1"
                     /translation="MKIAILSRNRTLYSTRRLVEAAQQRGHEVRVLDVLRCYMNITSL
                     RPSIHYKAEALEGFDAVIPRIGASVTFYGAAVLRQFEVMGVYPLNESVAISRARDKLR
                     SLQLLAREGVGLPVTGFAHSPDDIHDLLDEVGGAPLIIKLLEGTQGIGVVLAETQQTA
                     ESLIQAFMGLKTDILVQEYIAEAEGADIRCFVIGDEVVAAIKRQAKPGEFRSNLHRGG
                     RATLVELTDEERALAIRSARIIGLNTCGVDILRARRGPLVMEVNASPGLEGIEQATGL
                     DIAGMMIGFVERHARPHRTGTQGQG"
     gene            complement(14682..15134)
                     /locus_tag="PKI23_05035"
     CDS             complement(14682..15134)
                     /locus_tag="PKI23_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HNN36338.1"
                     /translation="MKHENGGDCLIEVGWREWIALPQLGIDRIKAKVDTGARTSALRA
                     SAIEPYPQRGANRVRFTVHPSQVDRQIERQCDAEVVDRRWVSDSGGHRELRYVIVTEL
                     LIPERRWSIEVTLTARPGMRFCMLLGRTAIVDRFTVDPARSYLLGRGG"
     gene            15369..16523
                     /locus_tag="PKI23_05040"
     CDS             15369..16523
                     /locus_tag="PKI23_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNN36339.1"
                     /translation="MHSDPFMLALVAAVLGILLVGMASQRLKQPHVVGYLLAGVMLGP
                     DGFALVTAPAIPERMGEIGVMLLLFFVGLEATPRQLFAHWRIALFGPLLQSAAALLLI
                     WPLGLWLGWSLTQVLLFSFIITLSSTAVVLKTLADSGELDSRLGRRLVGILLIQDLAI
                     VPMLIILRYGAGTELQGFDPLLTLVGGVLLVLLLGWLYVRPRIEVPLPGWIRADPEMQ
                     VFAALSFALGLALLTGLLNLSTALGAFVAGMLIGASRDADWVHRHLEPFRVVFVAAFF
                     VSIGMLVDLDRLLEHLGQILLLVLALLGVNTSINALLFRLFGDTAKQSLYAGALLAQA
                     GEFSFVLAAFGREQQIIDESNYQLAVAVIALSLLLSPLWVQLIRRHTDPL"
     gene            16535..17611
                     /gene="corA"
                     /locus_tag="PKI23_05045"
     CDS             16535..17611
                     /gene="corA"
                     /locus_tag="PKI23_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNN36340.1"
                     /translation="MDYFDKQYHPPGTPPGTLPQDEFIVAAEVHSRLICYDDDHFEKS
                     EGIRLDEIEPGSHDPHQVRWLAIHGAVSGTLLRELGERFQLHPLTVEDMFNQGQRPKV
                     ELFPGYLFVILRQPLIMDGAITIGQVSLLLKENLLISVVSGETDPFGPVHERLEHAIG
                     KIRQRGADHLLYCLIDALIDLGFPLLEWFSERIERLEEEVIHLPSQQTLRELHALRRE
                     LLLLRQTLWPQREVVGRLLHEQEEWFQSETQRFMRDCHDHIIQIMELLETYREMTSGL
                     LELYLSSSNYRLSEVMKVLTIIATLFIPLTFIVGVYGMNFSVGEGPWSMPELRWRYGY
                     PVLWLVMLAITGGMLLYFKRKRWF"
     gene            17608..18420
                     /locus_tag="PKI23_05050"
     CDS             17608..18420
                     /locus_tag="PKI23_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36341.1"
                     /translation="MKAEEHDLPRQWWGDFAVADGESLCWQIGPMQLQWVNAGSQWRC
                     AYRRLADVDDESTVGWQLTRGDATPAFDENPQRFVFSQPMRHATVLPALADRSVITRP
                     TTALTIPPRQSALLLFSTPLWFQLRCAGVSQPLFEVPISRPSDTWFGPSTCEGELAYA
                     SRTHARLQGEALPPRPHRAVTPVRIENRIDAPLLIERISLPVTLLSLFVAADGMLWTE
                     EVTLRREQAGEMAALQVGQGAPLLAQAATLVQGPRNANRSGILLRAFAALFH"
     gene            18442..19296
                     /locus_tag="PKI23_05055"
     CDS             18442..19296
                     /locus_tag="PKI23_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNN36342.1"
                     /translation="MESVLKYIGHIEILPAVRTVAIALAGFVVARLVSGAVIRVLGKR
                     LDRQQLPILRRILYYGILLLFLMSALRELGFDLSVLVGAAGILTVAVGFASQTSASNL
                     ISGLFLIAERPFAVGDTIKVGEFTGEVLAIDLLSIKLRTFDNLYVRIPNEQMIKTEVV
                     NLTKFPIRRVDIAVGVAYREDLGLVKAMLLGLADRHPLCLEEPRPIVYFAGFGESSID
                     LKLLVWTRRENYLELWNSLHEQIKLEFDRNAVEIPFPNRTIHPAALTEPFPVRVVAGA
                     VREETGRT"
     gene            complement(19304..19906)
                     /locus_tag="PKI23_05060"
     CDS             complement(19304..19906)
                     /locus_tag="PKI23_05060"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNN36343.1"
                     /translation="MQRLRNIFLQGLAAVLPISVTLALLYWLATTAERFLSPAIHLLL
                     PDRYYLPGLGILCGIGLIFLLGLLLNAFVIRALFDWTERTIARIPLIKGLYSGVRDLF
                     GLFAAGNNRAIEEEQVVALELNGARMIGLVTARDIRLPRRDRPADELLVAVYLPFGYQ
                     IGGHTLFLPRERLEPLDMGVEEAMRLVLTGGIGATGQSRR"
     gene            complement(19998..22868)
                     /locus_tag="PKI23_05065"
     CDS             complement(19998..22868)
                     /locus_tag="PKI23_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN36344.1"
                     /translation="MRLRLRPLPLRWLLTFSIFALILPALLLTIHLSQRNGNAQIESY
                     HRQFLHHQLANLQIEVSEHLDENDVVGIGRQVALLATLPEIEKIALLDEHDQILLSPV
                     SGWRGRAIEAVTPHYRAASMQQVRRQNRGTITSNQEKMLTAYYPVRLPAARDTIAQGL
                     NGGIYVEYSLKEPLRQQWRTVLSQGLLVILIATSAGLLLLWLLRHWIERPIQALIEAT
                     QSMARGDFHPRFEPTPGVELVRLNHAFRRLGIALARNRQQLHLQQSRWLAHVQSMPLG
                     CIEYDTAFRITAWNPAAERIFGYSAVEAIGQSGHLLIPAELADGAETAWSRLLDHKGD
                     TYLNHANLTKEGRCILCDWHITLLTKADGTVIGAAALVEEVTQREASRQALTDSAQRY
                     EKLFNSVADGVIVLNQQGRIQSLNHAAEQIFGCSQRKIAGQSIDRMIPQFATTIRLDS
                     SLSQLNGAASAHGRRHELPGRRHDGSSMTLQIESTPLEFQGERLFSVVVQDISERKRN
                     EARIERLAFYDPLTELPNRMLLMDRLEQAILHARRSHKNGAVFFIDLDNFKTINDSLG
                     HPVGDQLLRQLAVTFRHLVRAEDVIARFGGDEFIMLLNELNEEPEQAAEEALRIAEKI
                     LHQFDTPRLVLGHELRVTASIGMTLFPADSEQPDELIQQADTAMYSAKSSGRNAVRAY
                     LPSMAIAARQRHQIENELRAALPREQLCLYYQPKIDLKSGRIAGFEALLRWLHPRQGV
                     VSPNDFIPILEESGMILEVGQWVIEQICRDLPSLQSAGLLDASRTVAFNVSARQFHQA
                     DFVQKISQTLAEQQVDPGLLECELTESLLLHDTEEAIDKMAALRALGLHLAIDDFGTG
                     YSSLAYLKMLPVDVLKIDRSFIRALPDDPDDVAIVETILAIARQFDFAVVAEGVESEE
                     QARFLRERGCGSYQGYLCTAPRPLPELLERFGTPGDGDLQSA"
BASE COUNT         4092 a         6961 c         7303 g         4654 t
ORIGIN      
        1 aaaccctggc tcaagcggct gcatatcgat ccgctgctgc tgttgctgct gctgctgttg
       61 gccggttatg gcctggtcgt gctctacagt gccggtggcg gcagcctcga tgccatcgaa
      121 cggcagggaa tgtcgctggg gttggcaggc gtgttgatgc tgatcgccgc acagatcgac
      181 gtcgccatcc tgcgtcgctg ggcgctggtg atccatctgg gggggacggt cctgctgatc
      241 atcgtgctgc tggcgggtga cgatgccaag ggggcgacgc gctggatcga tctgcaggtg
      301 ctgcggttcc agccggcgga gatcatgaag ctggcgatgc cgctggcggt ctgtcgctac
      361 cttggtgacc accatctgcc gcccactctc cccaccgttg ccgtgtcgat ggcgttgata
      421 gcggtgccta cactgttgat cgccaagcag cccgatctgg gttcggcgct gctgatcgcc
      481 agttcgggtc tctatgcgct ctttctggct ggcatccgct ggttgtggat tgcgcttgcc
      541 ggccttgcag gggtgttgct cgcgccggcc ctgtggttct tgctgaggga ctatcagcgt
      601 cgacgcatcc tgacgctgat cgatccggag gccgatccgc tggggtcgag ctggaacatc
      661 atccagtcga agatcgccat cggctcgggt gggctccacg gcaagggttg gcttgccggt
      721 acccagtcgc atctcgattt tctgccggaa agccacaccg acttcatctt tgcggtgctg
      781 gccgaggagt ttggtctggt cggcgtgttg ctgctgtttg gcctctattt gataattgtc
      841 gcccgtggtc tgtggatcgc ggccaaggca cccgatctgt ttcagcgtct gcttgccggt
      901 tcgatcaccc tgaccttttc tgtctatgcc ctggtcaatg tcggcatggt gagtggtgtt
      961 cttcccgtcg tcggcgtgcc cttgccgctg atcagccagg gcggcactgc gctggtcacg
     1021 ctgatgctcg gctttggcgt gttgatgtcg atacagaccc gtacaccttg atcaatcgtt
     1081 ggtagatcca ttcatgcccc ttgaatctcc ttgctttcag gcgcgctgca ttcgcgcgct
     1141 ccgtatgctg tcgatgacac tgttgctgtc tggacattgt ggtgcgcttt gggcagcgga
     1201 ttatctggag caggtcgaag tgcagcagtt catcaccgaa ctggtcgaac atcacaactt
     1261 ctctcgaccg gagctcgaag cgctgttcag ccaggtgcag cggcagcagc gggtgatcga
     1321 tctgatctcg aagccggccg aaaaacggct cgagtggttc gagtaccgcc gcatctttct
     1381 cgatcaggag cggattcggc agggcgtgca gttctggcgc gcccatgctg ccgagctggc
     1441 acgggccgag agtcagttcg gcgtcgaacc cccaatggtg gtggcgatca tcggcatcga
     1501 aacccgttat ggtcgcaaca ctggcaccac tccggtgctg gatgcgctca ccaccttggc
     1561 attcgactat cctccccgca gcaaattctt ccgtcaggag ctgctcaact atctgctgct
     1621 ggcgcgcgaa gagggcaagg atccacgctc gatcaagggc tcctatgcgg gcgccatggg
     1681 ctatggccag ttcatgccca gcagctatcg caactatgcg gtcgatttcg atggcgatgg
     1741 caaacgcgac atctggaaca accgcagcga cgcgatcggc agtgttgcca gctatttcaa
     1801 ccgccatggc tggcagcaca atgcacccgt ggtgatgacg gcactgccca gcgccgagaa
     1861 tcctgccttg gcgttgaacg acaccaaaaa gccgaccctg tcggtggcgc agttggctgc
     1921 tcagggagtt cgtgcccgtc aatgggtcga accggagcgc aaggccgtgg cattgcggct
     1981 gctgggagat gcaggacccg aatattggct ggcttttgac aacttttacg tcattacccg
     2041 ttacaatcag agccagttgt atgccatggc tgctcaccag ctcagtgaag caatccggca
     2101 gcactacatt gcaggagccg actgagtgca gcagaacaaa gccattcaac tgatcagcct
     2161 gctgattttc tccacactgc tggttgcctg cggctcccag ccgagaattc cgcccccgcc
     2221 gggcaccagc cgcatcgata ttccggtcac cgcacctgcg gctccacctg ccagcgcgga
     2281 accctacagc ccccgtggca accgctcacc ctatcaggtg ttcggccaga cctactatgt
     2341 gctgccgacc gcccaaggct atcaggccga agggactgcc tcctggtatg gcgagcagtt
     2401 ccatggtttg cagacatcga atggcgaaat ctacaacatg tacgccctga ccgcggcgca
     2461 caagagcctg ccgattccct gctatgcgcg catcacccat ctgggcaatg gccgtcaggt
     2521 ggtggtgcgc gtcaatgacc gcggcccctt ccatgatgac cggctgatcg acctctccta
     2581 cggtgcggcg ctccagctcg gttttgccga tgtgggcttg gcccaggtgc gcatcgaggt
     2641 gatcaacgca tcggcgacac cgttgagtcc cactgcagcg gtcgtcgcat cgacaccgaa
     2701 gcccaccagg gtttcggcgg agaatggagt gcctgcggtc atcaagcgca aaccaccggg
     2761 atcgacgacc gccgcgcggt ctgctgccga agctggagcg ggtctctacc tgcaggccgg
     2821 cgccttctcc aggcgcgaag ccgccgccgc gatggtggag cggttgacgg atgtggtgaa
     2881 gcacccgatc agcatcagca cggctgacac tgccaagcgg gaatcgctgt tccgggtgcg
     2941 cattggtccg attgcgtcac aggatgaagc gcagcagata cagagcctga tcagcaagca
     3001 tggcattgcc tcgagttttg ccttccggca gtgagcggcc gtacagtcgg cgccatcgcc
     3061 atttcgcggt agaatcccga cagtttgacc gcagcgacat gttgcccgct cagcgaccag
     3121 ccgagcagcc gcgactgaat tcgcgggagt gccagccact cccgcatcca tgcacgattt
     3181 catgactcat acccatgcct gcttctgttc atcgatttgc tccatttttt ttcattctgg
     3241 tactcatgtt gtctgcttcg accggcgcag aacagccagt gcccaccccc gcgcccgtac
     3301 cggtggcggt gattcccgga ccgccggcgc tggcggccag tgcctatgtg atgatggacg
     3361 ccgacagtgg tcgcatcctc gcggcgcaga atgaaaacga gcggatggca cccgccagcc
     3421 tgaccaagat cctcaccagc tacatggtgg ccgaagagct gcaaaagggc cggattcgcg
     3481 aaaccgatga ggtcaacatc agcatcaagg cgtggcagat gggtggctcg cgcatgtatg
     3541 tccgcgaagg caccaaggtg ccgctgctcg atctgctgcg tggcgtggtg atccagtccg
     3601 gcaatgatgc cagtgtggcg ttggcggagc atatctccgg cagcgaagag acctttgccg
     3661 aggagatgaa ccggatcgcc gccaagatcg gcatgaagag cagccacttc gtcaatgcga
     3721 ccggctggcc ctcccccgac cactacacca ctgcccatga ccttgcgctg ctggcgcggg
     3781 cgctggtcaa aaaccacccg gagcactaca acctctactc cgagaaatat tttcactaca
     3841 acaacatcaa ccagcccaat cgcaaccggc tgttgtggat cgacccgtcg gtcgacggca
     3901 tcaagaccgg tcacaccgaa gaggcgggtt actgtctggt ggcttcggcc aaacgtgaca
     3961 acatgcgact ggtcacggtg gtgctcggca ccagcagcga tcaggcgcgc accacggaga
     4021 gccagaagct gctcacctat ggcttccgct attttgagac ccacaagctt tacaacggtg
     4081 gtgaagcctt gcagcgggcc cgcgtctacg caggtgcaca gaacgaggtg gagctgggtc
     4141 tggcacagga tctccacgtg acagtgccgc gtgggcaata tgggtcgctg gcggccagcc
     4201 tggagattcc cggcatcatc aaggcgccgc tcgcggcagg gcagaacctc ggcaagatcg
     4261 tgctgaagct gggcgagaca ccgctgctgg agcagccgct gaccgtgatc aagccggtgg
     4321 aagagggcgg ctggttcaag cgtctgctgg atgcagtcag gctgttcttt ctgcagcttt
     4381 tcggtggaga cggcgcgggc aaatcatgac cctctgccgg gacgaccttt tcgcgctcag
     4441 tcagtcgctc tgagccgttc gccgaagaac gccagcaccc gatcgagcgc cacccgcgtc
     4501 gggtggccgg cctcatcgac caggtcattg gtcagcaccg agtgggactt gccggagatg
     4561 ccgtgagggt tgccggggcc ggagtcgatt tcgatcgcct cgaaatggtc acccagctgc
     4621 tcgcgcaggc catcgaatct ggcctgggtg cagatcggat cgtggctgaa acgcagcccc
     4681 agcacactca actgctcctg ttcgagtcga tttttgacct gactcaggct gactggatcg
     4741 atgtcgatgg cgctctttcg gcgttcggtg attggaaagg gcagggaggg ttgactcagc
     4801 accggtgcca gcacggccgg ttccagcatc atgcccaggg caaagccgcc ggtgaagcac
     4861 atgccgagcg cgccgacccc gggaccgccc agctcctgat gcagtgcccg cgccagcgcc
     4921 cgcagtggcg tgatgatcgg actcgatttg ccgagggcaa aggcggtgaa ctcgcggcgc
     4981 acgcaggcgc ccagcatcga gcggaacagg tagccgcccg aaagcggcgc gcccggcact
     5041 ccgaacagcc agggcaggca gacggtgaag ccggcatcgg ccacccggtc ggcaaaagcc
     5101 tgaacctggg gggtgatgcc gggaatctcg tgcatcacga tcacccccgg tccttggcca
     5161 cgccggtaga agcgcagtgc gctgtcgccg aactgctgct cgtggaaggc gtaacccggc
     5221 agctgcggaa tggtcgccat ctgctctgct ccgtacatgt gggtggtaat tggtgggccg
     5281 tgcccggtgc tggcctggcg cgtgcggctc aaccccgcct gggcttgccg agcagccggt
     5341 cggagatgat gcgctgctgg atctcggagg tgccctcgaa gatcttggtc aggcgggcgt
     5401 cacgccagta gcgctcgacc gcatgaagct tggtgtagcc ggccccgccg aagatctgca
     5461 acgcttccga agtgacccgc tcggccatct cggtggcgaa atatttggcc atcgaggcct
     5521 cctggtcgca gcgaccacca cggtcgatct ggtcgcagac gtgatacatc aactgccgtg
     5581 ccgcctcaat ctcggtggcc atcgtggcca gcttgaagcg gatcgcctgg aattcgccga
     5641 tcggccggcc gaactgctcg cgctgctgtg catacttcag cgcatcctcg agcgcgccgc
     5701 gcgccaggcc gatcgcccgg gcggcggtgt gggcgcgggc gatttcgagt ccgctggcgg
     5761 tcgaggtgaa ggcttcatcc tcattgccca tctggtactc gaccggcacc ttgcagtcgt
     5821 cgaagcgcag ctcccaggtc ttccagccga agtagccgat cttgggaatc ggcgaaccct
     5881 gacagccctg cggcaactcg ccgcgcggct tctcgacgat gaagcggccc agtcctttcc
     5941 acggtgccgt ggggtcgcgc tcgctggtgc gggccagcac actgatgtag tcggccccat
     6001 cggcgaaggt gcaccagtac ttgtttccgg tgatgtacca atggtcacca cggcgctcgg
     6061 cacggcagga gacgccggag aggtcggagc cgacattggg ttccgagagc gcggcggcgg
     6121 ccagatattc gccgcgggcc gccttgcggg tcagttcgat gcgcttctca cgcggcatgt
     6181 tgccgagtcc catcatgccg ccggagcggg cgatgatgct ggccacgctc atccagccac
     6241 gtgccagctc ttcggcgatc aggcaatatt cgaagcagcc aaggcccagg ccgccgaact
     6301 cctccttgat gcggatgccg aagtagccca tgtcggccat cttctgccgc agttcaagtg
     6361 gaatgtcgcc ctggatcggg tcaagcgcat tggcgaccgg cagcacctct ttcatgctga
     6421 actgccgtgc ggtttcctga atcatcagcc gctcttcgct catgtaaccc atcgttcatc
     6481 tccctgttat tgtgatgtca tccgagctgc acaatcataa tcaaagagtt ctgccgtgat
     6541 ccatcattcg gagtgcccga gcggtcgtga gtcaaggaaa aaacatgcag gccactgcgt
     6601 cgatccatta ctggattgca ccatccaccc gcagtgacga gatcgacaat ccaccactgc
     6661 cgaatgagct ccaccctgtc tcacaacgcg cccggcgcgg cgaactcaaa ctctatgaca
     6721 gcttcgactg gcgcctctgg ttcggcgggt tggcgctcta tcgtctggac gggtgttatc
     6781 acctgctgcc gaggagcggt ttccggctcg acctggctcg acccgatggc agtggttatt
     6841 cgcggccgat caacttcgcc gagcagttcc gtgagccgga gttgaggcag ccagtgcagc
     6901 ggattctcgc tccgcgtgcg ctgatcgaga tcgcctcttt ccagtgccga gagcggtctg
     6961 ttcaggtcag gaatggtgat gacaaggggg tcgccctgct gcactgtttc gaactttccc
     7021 atccgttgcc agatggcaga caggcgcgac tggagggtct ctggatcgca ccctgcatcg
     7081 gctatcagct cgagctggag atgatcgagg cgacactgcg ggcggcgggt tggcagcatg
     7141 aaatgcgcaa tccgctggag cttctgttgc ctgccgcaca acagccgcgt ccggagcgca
     7201 cccgaccgct gaccgacatg gtcggcgaag agccggcgga tgacctgctg cggcgtctgc
     7261 tcctcgccca gttgcgggtg gtcaaggagc aggaggatgg catcatcaaa cagatcgaca
     7321 gcgagttcct gcatgattac cgtgtcgcgc tgcgcaaggc ccggtcgctg ttgagtctgg
     7381 ccaaacgggt gtttcctgcc gcgattcagc aacgggcccg ccatgatttt gccgatctgc
     7441 aacgtcagag caatcagctg cgcgatctcg acgtctggct gctgcaacgg gaacattttc
     7501 agaaaatggt gccacctcgt ctgcaaaccg ggttcgaaca gctttttgcc gagcttcagg
     7561 cgacccggaa tcaggcgcag cggcggctgc gacgcttgct gaattccagt gcccatcaac
     7621 tgcggatgca gcagtggctg tatgatctgg cacagccggg gctctgggag gagcctgccg
     7681 aggtgaagag atcggcgcgc gcactgatcg atcggcagat cctgcgccgc catcgtcagt
     7741 tgctcaaggg tgcccagcgt ttttcccaac tggatgacga agaggttcac gacttgagaa
     7801 tcgaagtaaa aaaactgcgc tatctacttg aaatattttc ttatttctat tcgaaggagc
     7861 cctgtgactg gctgctcacc cacctgcgca aggtgcaggg caggctcggc gattacaacg
     7921 atctgcaagt acagcagacc catctgcaac actttgccga aacgctgatc gtcggggaaa
     7981 aatccaccgg tgaggtattg cttgctgccg gtgccatcgt tgccggtctc gagcgtgaaa
     8041 aacagcaggc gcgcaagcgc attgcagaac cgctgcacag actggcgaca cccaatggcc
     8101 gggcgcagtt gcagcagttg ttgaacgagc aatgaattga tgaaggtcat cgcctgttac
     8161 agcatcaagg ggggtgtcgg caagaccgcc acggcggtca acctggccca tgctgccgca
     8221 accagcggct accgcacgct gctggtcgat ctcgatccac aggcggcggc gaccttctat
     8281 tttcggatca aggcgcccga agagaagaag gttccgcgct ggttcaggac gggccaggat
     8341 ctgcaacagg cgattcgtgg cagcgactac ccgcgtctcg acctgctgcc ggctgatctg
     8401 ggctatcgcg acagtgaggc catgctggct ggcatgaagc gctccgacaa acagttgcgc
     8461 aagcggctca aggaggtggc cgacgggtac gacatggtgc tgatcgattc gcccgccaac
     8521 atcggtctgc tgtcggagaa catctttcgg gcggccgatg tggtccttgt gccggtgatt
     8581 cccaccaccc tgtcggagcg caccctgcgg cttctgctgc atttcttcgc gaaacagaag
     8641 ctcgatgtcg atcggctgct gccgttcttc tcaatggtgc agcctcacaa gcgactgcat
     8701 gccgacacca tggcacgctt gcgtgcggag tttccgcaac tgctgcagag caccattccc
     8761 ttcaccgtcg acatcgagaa catgggcgtg ctgcgcgaac cggtgctggc ctatgcgccg
     8821 cgccgtccgg ccggtgaagc ctatcgcgcc ctgtggctcg agctgctcgg gcgtctcatt
     8881 ccccggcagg agtgatggca ttgcatcacc gcagacagtg gagtagcctc ggagcggtcg
     8941 agccggggtg tggcgaaatc ctgtcgtcat cttcatcggc catggtgcac cgaatccgtt
     9001 ggttgctcag gaggaggcat tggcccaatc gtcatcgcca gtcctgtcgg gtgagcgaat
     9061 tctgcggctg aatctggccg ggcagccgat cgaatggctg acctggcagg aggcggtctc
     9121 gctgtatgcc cgtgatctgg tgggctggac gctgggcgag gtggtctgca ccctgcgcgg
     9181 tggaatctca cgcctgagcc agcgccgcag tcgtctcgaa ctgcacagca tcatcgcctg
     9241 ccgcggcaaa atccacgatc catgccaata tgaaccgccg ctgaccaacc gtggactgtt
     9301 ccggcgcgac cagaatctct gtctctactg cggcaagtcg ctctccgacc ggctgctgac
     9361 ccgcgatcac ctggtgccga cgtcccgcgg cggacgggat gagtggacca acgtggtcac
     9421 cgcctgccgc agttgcaatc agggcaaggg caatcgtctg ctgcatgagg cgcgcatgga
     9481 gctgctggcc ctgccttatc gccccaactg ggcggaatat ctggcactcg agaacagcgg
     9541 ccgggtacgt ggtgaccaga tggaatttct gcgccggcag ttttcaccgc actgccgctt
     9601 tgcttgatca acccggatga tctttgggtg gagtcgtttt caaaaatgga tgttgaaatc
     9661 gttctgaaag aaaaatttct gcatgtcagg gcgagcggac actattcgct cgatgagatg
     9721 aacgccatga gccgggcgat gtttgccgca ctccagcaac atccgcgggc gagctgcctg
     9781 atcgattgta gccaggtgac cggctcgcca accctgaacg agcgattcga atggccaagg
     9841 ttcaccgcgc gactgatcga agacttgcca gcgatccagc gccagacccg atttgccttt
     9901 gtcgcttcgg agccttttct cgatccaggg cgctttggtg aaaccgtggc cgtcaaccgg
     9961 ggcgtgaacg tcgtggcgac cgacagtctc gacgaggcat tgcgctggct gggtgtcgaa
    10021 acaggaagcg catgaaagta gagatcaccc cgaaagagca ttacctgcat gtcagggcag
    10081 ttggagagtt ttcagttgcc ggcatgaagg caatgagtct ggccatcatc gctgcgctgg
    10141 tcgagcattc aagctcgaaa tgtctgcttg actgtcgaca ggtggtgggt gcgcggacgc
    10201 tggccgagcg tttctcttgg atagagtttg ctgccgatga gtcgagcaaa ctgccgttcg
    10261 cttcaaaccg ggcccgcatt gcctatgtcg gcgccgcaaa tcggctggat cctggccgtt
    10321 ttggagaaac ggtcgcgatc aatcgggggt tgaatgtgat ggtgaccgac agcctcgacg
    10381 aggcgctgcg ctggctcgat ctgcaggccg attgaacggc aaccacccaa tactggtgca
    10441 gacgcacatc ggccggatca gctacttgcc gaggtaggag accccttcgt ccagcccgcg
    10501 cagcgtgagc ggaaacatcc gcccatcgag cagttgattg accatctgca cgctgttgga
    10561 gtactcccag gtgtcctgcg cctgtggatt gagccagatc acccgctcgt aggtctcgac
    10621 gacgcgttgc atccaggtgg cgccggactc ctcgttgttg tgctcgatgc tgccgccgat
    10681 gttggtgatc tcgtaggggg ccatggcggc atcaccgacg aaaatcacct tgtagtctga
    10741 cgagaacttg tgcagcagat cccaggtggc ggtgcgctca tgcatccgcc gggtgttgct
    10801 cttccacacc gattcataga tgaagttgtg gaagtagaaa tactccagat gtttgaactc
    10861 ggtgcgcacc gccgagaaca gcccttcgca gattcgcacg tagggatcca tggagccgcc
    10921 gacgtcgaag aagatcagca ccttgaccgc gttgtgccgc tcgggaacga acttgaggtc
    10981 gagcaggccg ccacgcttgg cggtggcatc gatggtctca tcgagatcga gctccacttc
    11041 ggcaccgctg cgggcgaacc tgcgcagccg gcgcagcgcc atcttgatgt cgcgggtgcc
    11101 cagttcgacg gtgtcgtcca gatccttgaa ctgacgggtc tcccacacct tggcggcgcg
    11161 gaagttgcgg tttccatcct ggccgatgcg aattccttcc gggttgtagc cctgggcgcc
    11221 gaacggcgag gtgccgccgg tgccgatcca cttgttgccc ccctcatgcc gctccttctg
    11281 ctcctcgagt cgcttcttga actcctcgat cagtttttcc agtccgccga gcgactcgat
    11341 cttcgcccgc tcctcggggg tcagcgactc ctcgaaggcc ttgcgcagcc actcttcggg
    11401 aatgaacgcg gcgatgatgc cctcgagggt ttcgagcccc ttgaagtagc ggccgaaggc
    11461 gacatcgaaa cggtcgaaat gggtctcgtc cttcaccatg atcgcacggc tcaggtagta
    11521 gaattcatcg agatcgccat agaccagccg ggcgttcatc gcttcgagca gatccatgta
    11581 ttcgcgcagc gacactggaa tgttgctgcg cttcaaggcc atgaagaagt cgatgagcac
    11641 ggctcagccc cggctctggc gacggtgcat gaaggccagc cgttcgagca ggtgcacatc
    11701 ctgctcattc ttgaccagtg caccatagag cggcgggatg gccttggcgg catccttgtt
    11761 cttcagcacc tcctcgggca tgtcgtcggc catcagcagc ttgagccagt cgatcagttc
    11821 ggaggtcgag ggttttttct tcagtccagg cacggtgcgc acatcgaaga aaatttccag
    11881 ggcctctttc accaactcct gcttgatctc cgggtagtgc accttgacga tctggatcat
    11941 cgtttcacgg tcggggaagg agatgtagtg gaagaagcag cggcgcagaa aggcgtcggg
    12001 cagctctttt tcgttgttgc tggtgatgat cacgatcggc cgcctgcgtg ccaccacccg
    12061 ctgctgggtc tcgtagacgt agaactccat ccggtcgagt tcgagcagca ggtcattggg
    12121 aaattcgatg tcggccttgt cgatctcatc gatcagcagc accacctgct ggtcggcgtc
    12181 gaacgcctcc cacagcttgc ccttgacgat gtagttggag atgtcatgca cccgcgcctc
    12241 acccagttgc gagtcgcgca gccgcgacac ggcgtcgtat tcatagagcc cttgctgcgc
    12301 cttggtggtc gatttgatgt gccatttgat gatcggcagc ttgagtgcct cagccacctc
    12361 ttcggccagc atggtcttgc cggtaccggg ttcacccttg atcagcagtg ggcgttgcag
    12421 ccggatggcg gcgttgaccg ccatctgcag atccttggtg gcgacgtagg attgggtgcc
    12481 ttcgaaattc atgggcgatg ttcccgttcg ttgtgcaggt ggatagctat taacttttcg
    12541 actttaaagc agatcattga gccggatcaa tatgccggag tgttgaatca cggtggcaat
    12601 tctccatact gacgccctgc attaacccaa ggcgcactcg cgccctgttc aggaggtagt
    12661 ctgtcgatgt cgtctcccat tgaaaccgat gccgtgatcg ttggtgccgg tcccgtcggt
    12721 ctgtttcagg tgtttgaact cggtctgctg gatatccgtg cccatgtcat cgaatcgctg
    12781 ccccaggtgg gtggacagtg ctcggagctc taccccgaca agccgatcta tgacatcccg
    12841 gcgctgccga tctgtggcgc ccaggagctg atcgaccggt tgatggagca gatcagaccc
    12901 ttttcgccga ccttccatct ggggcaggag gtcaacaagg tgcaacagcg tgaggatggc
    12961 cgtttcgatg tcggcaccag cgccggcacc cagttcatcg ccggcaatgt gatcatcgcc
    13021 ggtggggtcg gctcgttcca gccggtgcag gtcgaggtgc ccggcatcga ccggctgctc
    13081 gaccactcgc tcttctatcg ggtgaaggat cctgcccggc accatggcaa gcggctgctg
    13141 gtggtcggcg gtggcgattc ggcgctcgac tggaccctga gcctttgcga ccaggcgcag
    13201 tcgctgacgt tgattcaccg tcgcaacgag tttcgcgccc agcccaactc ggtcaatcag
    13261 atgcatgaac tctgcgcggc cggcaagatg aatttcaagg tggcggtggt ggccgatttc
    13321 gaggccgatg gcacccagct gaagcgggtg gcgctcaaaa gtgggcaggg cgagccggag
    13381 tggatcgagt gtgaccacat tctggcactg ctggggctga ccccgaatct gggcccgatt
    13441 ggggagtggg ggctggagat cgtccgcaag caggtggtgg tcgacaccga aaagttccag
    13501 accaatgtgc cgggcatctt cgcggtgggt gacatcaaca gctatccggg caagaagaag
    13561 ctgattctga gcggctttca cgagaccgcg ctggctgcct ttgcgatcaa ggagcggttg
    13621 cagcccggca agaaggtgca cctgcagtac accaccacca gcccggcgct gcaggagcgg
    13681 ctcggggtgc gcggcagcga cggtgagacg tcgacgcaat agaaaaaagc gaacgcggtc
    13741 agggatggcg gcggcttatc cctgaccctg agtcccggtc cggtggggcc gggcatgccg
    13801 ttcgacgaag ccgatcatca tgccggcgat gtcgagcccg gtggcctgct cgatcccttc
    13861 gagaccggga gaggcgttca cctccatcac cagcgggcca cgccgggcac gcagaatgtc
    13921 cacaccgcag gtgttgaggc cgatgatccg cgccgacctg attgccaggg cgcgctcttc
    13981 gtcggtcaac tcgaccaggg tggcacggcc gccgcggtgc aggttggagc ggaactcgcc
    14041 cggtttggcc tgtcgcttga tggccgccac cacctcgtcg ccgatcacga agcagcgaat
    14101 gtcggcccct tcggcctcgg cgatgtactc ctgcaccaga atgtcggtct tcagccccat
    14161 gaaagcctgg atcaggcttt cggcggtctg ctgggtctcg gccagcacca ccccgatgcc
    14221 ttgggttcct tcgagcagct tgatgatcag tggtgcaccg cccacttcgt cgagcagatc
    14281 gtgaatgtcg tccggtgagt gggcgaaacc ggtcactggc agccccaccc cttcgcgtgc
    14341 cagcagttgc agagaacgca gcttgtcgcg tgcgcggctg atggccaccg attcgttgag
    14401 cggatagacc cccatcacct cgaattgccg cagcacggct gcaccataga aggtgaccga
    14461 agcgccgatt cgcgggatca ccgcatcgaa gccctccagc gcctcggcct tgtagtggat
    14521 gctcgggcgc agcgaggtga tgttcatgta gcagcgcagc acatcgagca cccgcacctc
    14581 atgtccacgc tgctgggcgg cctccaccag tcggcgtgtc gaatagagcg tgcggttgcg
    14641 ggagagtatg gcgattttca tcagcgttgc cttgcgggag gtcagccgcc gcgaccgagc
    14701 agataggagc gtgccggatc gaccgtgaac cgatcgacga tcgcggtgcg ccccagcaac
    14761 atgcagaagc gcatgcccgg ccgggcggtc agcgtgactt cgatcgacca gcgtcgctcg
    14821 ggaatcagca gttcggtgac gatcacatag cgcagttcgc gatggccgcc ggagtcgctg
    14881 acccagcgcc ggtccaccac ctcggcatcg cactgccgtt cgatctgtcg atcgacctgt
    14941 gacggatgga cggtgaagcg gacccggttg gcgccgcgtt gcgggtaggg ttcgatggcc
    15001 gaggcgcgca gtgccgaggt gcgggcgccg gtgtcgacct tggccttgat gcggtcgatg
    15061 cccagttggg gcagggcgat ccactcgcgc cagcccacct cgatgaggca gtcgccgccg
    15121 ttttcatgtt tcaccggccg atcttcctct gctgactgaa tccgaaaggg cttcgcatga
    15181 acggtgcagg tgtcgcatgg tctgattcgt gtcgggtaag aagcgctgga tggtggttgc
    15241 tgcatcgaga ctccctctgt cgtctgttgc gcatccggcc ccctcgtttc gatgcggtgc
    15301 gggctaaaat agcgcgagtt tcaaaatttg cccatctttc ggcactgaat aagcctgtag
    15361 agcagaccat gcattccgat cccttcatgc tggcgctggt ggccgccgtg cttggcatcc
    15421 tgctggtggg gatggcctca caacggctga agcagccgca tgtggtcggc tatctgctgg
    15481 caggcgtcat gctcgggccg gatggtttcg ctctggtgac ggcgccggcg attcccgagc
    15541 gcatgggcga gatcggcgtg atgctgctgc tgttcttcgt cgggctcgaa gcaacacccc
    15601 ggcaactctt cgcccactgg cgcatcgccc tgttcggtcc actgctgcaa agcgcggcgg
    15661 cgctgctgct gatctggccg ctgggtctgt ggctcggctg gagtctgacc caggtgctgc
    15721 tgttcagctt catcatcacg ctgagcagca ccgccgtggt gctgaagacg ctggccgaca
    15781 gcggtgaact cgacagccga ctgggccgcc ggctggtcgg tattctgctg attcaggacc
    15841 tggccatcgt gccgatgctg atcatcctgc gttatggggc cggcaccgaa ttgcagggat
    15901 tcgacccgct gttgacgctg gtgggtggag tgctgctggt gctgctgctt ggctggctct
    15961 atgtcagacc acgaatcgaa gtgccgctgc cgggctggat tcgcgccgac ccggaaatgc
    16021 aggtgttcgc cgccctgagc tttgcactgg gcctggcgct actgaccggg ttgctcaacc
    16081 tctccactgc gctgggggcc tttgtggccg gaatgctgat cggtgccagc cgcgacgccg
    16141 actgggtgca ccgccatctg gagccgttcc gggtggtgtt cgtggcggct ttcttcgttt
    16201 cgatcggcat gctggtcgat ctggaccggt tgctggagca cctcgggcag attctgctgc
    16261 tggtgctggc actgctcggc gtcaacacct cgatcaatgc actgctgttc cggctgttcg
    16321 gtgacactgc caagcagagt ttgtatgctg gcgcgctgct ggctcaggct ggcgaattca
    16381 gcttcgtgct tgccgccttc ggtcgggagc aacagatcat cgacgaatca aactaccaac
    16441 tggcggtcgc ggtgattgcg ctgtcgctgc tgctgagtcc attgtgggtg cagctgatcc
    16501 ggcggcatac cgatccactc tgaagagatg gccgatggac tatttcgaca aacagtacca
    16561 cccgcccggc acgccgcccg gcaccctgcc ccaggatgag ttcatcgtgg ccgccgaggt
    16621 gcactcccgg ttgatctgct atgacgacga ccatttcgag aagagcgagg gcattcgact
    16681 cgatgaaatc gagcccggca gccatgatcc gcaccaggtg cgctggctgg ccattcatgg
    16741 tgcggtgagt ggcacactgc tgcgcgagtt gggtgagcgc ttccagttgc atccgctgac
    16801 ggtcgaggac atgttcaacc aggggcagcg gcccaaggtg gagctctttc ccggctatct
    16861 gttcgtgatt ctccggcagc cgctgatcat ggatggagcg atcaccatcg gtcaggtgag
    16921 cctgctgctg aaggagaatc tgctgatcag cgtggtcagc ggtgagaccg atccattcgg
    16981 tccggttcat gaacgcctcg aacatgccat cggcaagatt cgccagcgtg gtgccgacca
    17041 tctgctctac tgtctgatcg acgcattgat cgatctgggc tttccgctgc tggagtggtt
    17101 cagcgagcgg atcgaacggc tcgaagagga ggtgattcac ctgccgagcc agcagaccct
    17161 gcgcgaactg cacgcgctgc gccgtgagct gctgctgctg cgacagacgc tctggccaca
    17221 gcgtgaggtg gtgggccgcc tgctgcatga gcaggaggag tggttccaga gcgagactca
    17281 gcgtttcatg cgcgactgcc atgaccacat cattcagatc atggagctgc tcgagaccta
    17341 ccgcgagatg acctcaggcc tgctggagct ctatctgtcg agcagcaatt accgattgag
    17401 cgaagtgatg aaggtgctga ccatcatcgc cacgctgttc attccgctca ctttcatcgt
    17461 cggtgtctat ggaatgaact tttcggtggg cgagggcccc tggtcgatgc ccgagctgcg
    17521 ctggcgctac ggctacccgg tgctgtggct ggtgatgctc gccatcaccg gcggaatgtt
    17581 gctctacttc aagcgcaaga ggtggttttg aaagctgaag agcatgacct gcccaggcag
    17641 tggtgggggg atttcgcagt tgccgacggc gagagcctct gttggcagat cggtccgatg
    17701 cagttgcaat gggtgaacgc cggttcgcag tggcgctgcg cttaccgccg gttggccgat
    17761 gtggatgacg agagcacagt gggctggcag ctcacccgcg gtgatgcaac ccctgcattc
    17821 gatgagaatc cgcagcgctt tgtcttctcg cagccgatgc gtcacgcaac ggtgctgccg
    17881 gcgctggcgg accgatcggt catcacccgg ccgaccacgg cgctgacgat tccgccgcgg
    17941 cagtccgcgc tgctgctgtt cagcactccg ctgtggtttc agttgcgttg tgctggcgtg
    18001 tcgcagccgc tgttcgaggt gccgatcagc cgcccctccg acacctggtt cggcccctcg
    18061 acctgtgaag gcgagctggc ctatgccagc cgcacccatg cgcggttgca gggcgaggca
    18121 ctgccaccgc ggcctcaccg cgcggtgacg ccggtgcgga tcgagaaccg gatcgacgca
    18181 ccgctgctga tcgagcgcat cagcctgccg gtgacgttgc tgtcgctgtt cgtcgcagca
    18241 gacggcatgc tctggaccga ggaggtgacc ctgcgtcgcg aacaggccgg cgagatggcc
    18301 gcgctgcagg tggggcaggg tgccccgctg ctggcacagg cggccaccct ggtgcagggg
    18361 ccgagaaacg ccaaccgatc gggcatcctg ctgcgggcct ttgccgcgct gttccactga
    18421 aaaaatagcg tgatcaatat gttggaatct gttctgaaat acatcggtca cattgaaatc
    18481 ttgcccgccg tgcggacggt ggcgattgcc ctggccggtt tcgtggtggc acgtctggtc
    18541 agcggtgcgg tcatccgggt gctgggcaag cggctcgacc gccagcaact gccgattctg
    18601 cgacgcatcc tttactacgg cattctgctg ctgtttctga tgtcggccct gcgcgagctg
    18661 ggtttcgacc tcagcgttct ggtcggggct gccggcatcc tgacggtggc ggtcggtttt
    18721 gcctcgcaaa cctcggcatc caacctgatc agcggtctgt tcctgattgc cgagcgcccc
    18781 tttgcagtgg gcgacaccat caaggtgggg gagttcaccg gcgaggtgct ggcaatcgat
    18841 ctgctgtcga tcaagctgcg caccttcgac aacctctatg tgcgcattcc caacgaacag
    18901 atgatcaaga ccgaggtggt caacctgacc aagttcccga tccggcgggt cgacattgcc
    18961 gtgggggtgg cctaccggga ggatctcggt ctggtcaagg cgatgctgct gggattggcc
    19021 gaccgccatc cgctctgtct cgaagagccg cgacccatcg tctatttcgc gggttttggc
    19081 gaatcgtcaa tcgatctgaa gctgctggtc tggacccggc gcgagaacta cctggagctg
    19141 tggaactccc tgcacgagca gatcaagctg gagttcgacc gcaacgcggt cgagattccc
    19201 tttccgaacc gcaccattca cccggcggcg ctgaccgagc ctttcccggt cagggtggtg
    19261 gccggcgcgg tcagggagga gaccgggcgc acctgaaatt tcttcagcgc ctggattgcc
    19321 cggtcgcgcc aatgccgccg gtcagtacca ggcgcatcgc ctcctcgacc cccatgtcga
    19381 gcggttcgag ccgctcgcgc ggcagaaaca gcgtgtggcc gccgatctga tagccgaagg
    19441 gcagatagac cgcgaccagc aactcatcgg ccggtcgatc gcgccggggc agccggatgt
    19501 cgcgggccgt caccaatccg atcatgcgtg cgccattcag ttcgagggcc accacctgct
    19561 cctcctcgat ggcgcggttg ttgccggccg cgaagagtcc gaacagatcg cgcaccccgc
    19621 tgtagagccc cttgatcagc ggaatccgcg cgatcgtgcg ttcggtccag tcgaacagcg
    19681 cgcggatcac gaaggcgttc aacagcaacc cgagcagaaa gatcagtccg attccgcaca
    19741 gaatgccgag tccgggcaga tagtagcgat cgggcagcag cagatggatg gccggactca
    19801 gaaagcgttc ggcggtggtg gccagccagt agagcagggc cagggtgacg ctgatcggca
    19861 gcaccgccgc cagaccctgc agaaagatgt tgcgcagtcg ctgcatcgtc atgtccatga
    19921 caggtagagg agcgcgtcag tttgcctgag tgccggggcg ctttcgagtt ttgttgtttg
    19981 attgacgcag tgcaggttca agccgattgc agatcgccgt cacccggcgt tccgaagcgc
    20041 tccagcagct ctggcagtgg gcgcggtgcg gtgcagaggt aaccctgata gctgccgcag
    20101 ccgcgttcgc gcaggaaccg tgcctgctct tcgctctcca ctccctcggc caccacggca
    20161 aaatcgaact ggcgggcgat ggcgaggatg gtctcgacga tggcgacatc atcggggtcg
    20221 tcgggcagcg ccctgatgaa ggagcgatcg atcttcaata catcgaccgg cagcatcttc
    20281 aggtaggcca gcgacgaata gccggtgccg aaatcatcga tggccagatg cagacccagt
    20341 gcccgcagcg cggccatctt gtcgatcgcc tcttcggtgt cgtgcagcag cagactctcg
    20401 gtcagctcgc actccagcag cccgggatcg acctgctgtt cggcaagggt ctgactgatt
    20461 ttctgcacga agtcggcctg gtggaactgc cgggccgaca cattgaaggc gacggtgcgt
    20521 gacgcatcga gcaggccggc tgattgcaaa gacggcagat cacggcagat ctgctcgatg
    20581 acccactgcc ccacctccag aatcatgccg ctctcttcca gaatcggaat gaagtcgttg
    20641 ggtgacacca ctccctgtcg tggatggagc cagcgcagca gcgcctcgaa accggcgatc
    20701 cggccgctct tcaggtcgat cttcggctgg tagtagaggc agagctgctc ccgcggcagg
    20761 gcggcacgca gctcattctc gatctgatgg cgctggcgtg cggcaatggc catgctcggc
    20821 aggtaggccc gcacggcgtt gcggccgctc gacttggcgc tgtacatggc ggtgtcggcc
    20881 tgctggatca gctcatcggg ctgctcgctg tcggccggaa acagcgtcat gccgatgctg
    20941 gccgtcacgc gcaactcatg gccgagcacc aggcgtggtg tgtcgaactg atgcaggatt
    21001 ttttcggcga tgcgcagcgc ttcttcggcg gcctgttcgg gctcttcatt cagctcattg
    21061 agcagcatga tgaactcatc accaccgaaa cgggcgatga catcctcggc gcgcaccaga
    21121 tgtctgaagg tcaccgccag ttgccgcagc aactgatcgc ccaccggatg gccgagggag
    21181 tcgttgatcg tcttgaagtt gtccagatcg atgaagaaca ccgcgccgtt tttgtgactg
    21241 cgccgggcgt ggagaatggc ctgctccagc cggtccatca gcagcatgcg gttgggcagt
    21301 tcggtgagcg ggtcatagaa ggccagccgc tcgatgcgtg cttcattgcg cttgcgttcg
    21361 ctgatgtcct gcaccacgac cgagaagagc cgttcgccct ggaactccag cggcgtgctt
    21421 tcgatctgca gcgtcatgct gctgccatcg tgacgcctgc ccggcagctc atggcgtcga
    21481 ccgtgtgccg acgctgcgcc gttgagttgg ctgagggagc tgtcgagtct gatcgtggtg
    21541 gcgaactgcg gaatcattcg gtcgatcgat tgtccggcga tcttgcgctg cgaacagccg
    21601 aagatctgct cggcagcgtg gttgagtgac tgaatgcggc cctgttgatt cagtacgatc
    21661 accccatcgg ccaccgagtt gaacagcttc tcatagcgct gtgcgctgtc ggtcagtgcc
    21721 tggcggctgg cctcgcgctg ggtcacctcc tcgaccagtg cggccgcgcc gatgacggtg
    21781 ccatctgcct tggtcagcag cgtgatgtgc cagtcgcaga ggatgcaccg tccctctttg
    21841 gtaaggttgg catggttgag gtacgtgtct cccttgtggt cgagcagcct gctccaggcg
    21901 gtctctgcgc catcggcgag ctccgcagga atgagcaggt gaccgctctg gccgatggcc
    21961 tcgacggcgc tgtagccgaa aatgcgttcg gcggcggggt tccaggcagt gatccggaat
    22021 gcggtgtcgt actcgatgca gcccagcggc atgctttgga catgagcaag ccagcggctc
    22081 tgttggagat gcagttgctg gcggttgcgc gccagtgcga tgcccaggcg tctgaaggca
    22141 tgattcagtc tgaccagttc gacaccgggt gtcggctcga aacgggggtg aaagtcgcct
    22201 ctggccatgc tctgggtggc ctctatcagc gcctggatcg gccgctcgat ccagtgacgc
    22261 agcagccata acaacaacag accggcactg gtcgcgatca gtatcaccag cagaccttgc
    22321 gacaggacgg tgcgccactg ctgccgaagc ggttctttca ggctgtattc gacatagatg
    22381 ccgccgttca gcccctgggc gatggtgtcg cgtgccgcag gcaatctgac cgggtagtag
    22441 gcggtcagca ttttttcctg attcgaggtg atggtgccgc ggttctgtct gcgcacctgc
    22501 tgcatcgagg ctgcccggta gtgcggggtg acggcctcga tggcacggcc gcgccagccg
    22561 gagactggac tcagcaggat ctggtcatgc tcgtcgagca gcgcgatctt ctcgatttcg
    22621 ggcagggtgg ccaaaagtgc gacctgccgg ccgatgccga cgacgtcgtt ttcgtccaga
    22681 tgctcgctga cttcgatctg caggttggcc agttggtgat gcaggaattg gcggtggtag
    22741 gattcgattt gcgcgttgcc attgcgctgc gacaaatgga tggtcaacag cagcgcaggc
    22801 agtatcaacg caaaaatgct gaaggtcagc agccagcgca gcggcagcgg gcgcagtcgc
    22861 agtctcattg ggcaggctcg gcgacaaaat catcggcgat caggccatca aaccgctggc
    22921 ccgctggcaa cagatcggcg gccgccatga tgtcgctcac ctggcgcacc gtcgcctcga
    22981 agggcggtgc agtgccgcac agcaatgcgc
//
LOCUS       DAORCQ010000032        21284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1427_length_21284_cov_6.542701, whole genome shotgun sequence.
ACCESSION   DAORCQ010000032 DAORCQ010000000
VERSION     DAORCQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21284
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1427_length_21284_cov_6.542701"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..487
                     /gene="rpsJ"
                     /locus_tag="PKI23_05070"
     CDS             176..487
                     /gene="rpsJ"
                     /locus_tag="PKI23_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNN36345.1"
                     /translation="MQNQRIRIRLKAFDHRLIDVSAQEIVETAKRTGAQVKGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTYKRLLDIVDPTDKTVDALMKLDLAAGVDVQISL
                     G"
     gene            604..1239
                     /gene="rplC"
                     /locus_tag="PKI23_05075"
     CDS             604..1239
                     /gene="rplC"
                     /locus_tag="PKI23_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNN36346.1"
                     /translation="MSIGLVGRKSGMTRLFTPAGESIPVTVLEVSPNRVVQVKSAARD
                     GYCAVQVTTGSRKASRVAKAEAGHFAKANVEAGRGLWEFVIEGESELVPGGELDLSIF
                     EVGQHVDVTGVSKGKGFAGVVKRWNFRTQDATHGNSLSHRAPGSIGQNQTPGRVWKGK
                     KMAGHMGAEQVTALNLEIVKIDLEKNALLVRGAVPGAAGGDLIVRPSVKSK"
     gene            1248..1871
                     /gene="rplD"
                     /locus_tag="PKI23_05080"
     CDS             1248..1871
                     /gene="rplD"
                     /locus_tag="PKI23_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNN36347.1"
                     /translation="MELEVKSFVDGEIPSGSISLSDQVFGQEFNESLVHQIVVAYLAG
                     GRQGSKGQKTRAEVRGGGKKPWRQKGSGRARAGSSRSPLWRSGGKSFAATPRDHGQKV
                     NRKMYRAALRSIFSELVRQERVTVLADFSLESPKTKNLLEKLSGIQEQKILIVTLEFE
                     NNLYLASRNLIGVDVLEADSVDPVSLVGSERILVTLSALKKIEEALA"
     gene            1868..2164
                     /gene="rplW"
                     /locus_tag="PKI23_05085"
     CDS             1868..2164
                     /gene="rplW"
                     /locus_tag="PKI23_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011169628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNN36348.1"
                     /translation="MNQERIYSILLSPHVSEKAAGVAGKYNQYVFRVAKNSNKREIHK
                     AVETLFKVKVSSVQTLNVKGKVKRNRHGLVNRPDWKKAYVLLEPGNDIDFTSIG"
     gene            2179..3006
                     /gene="rplB"
                     /locus_tag="PKI23_05090"
     CDS             2179..3006
                     /gene="rplB"
                     /locus_tag="PKI23_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNN36349.1"
                     /translation="MAVLSKKPTSPGRRFQISVTNPELHKGAPCAALLEKKRKNGGRN
                     NAGRITVRHQGGGHKQHYRVIDFRRDKDSIPSVVERIEYDPNRSAHIALLKYRDGERR
                     YIVAPKGLKAGDEVVSGPLAPIKAGNALALRSIPLGSLIHCVELKPGKGAQLARSAGA
                     SIQLVAKEGVYATLRLKSGEMRKVSIECRASIGEVSNSEHNLRSLGKAGASRWRGRRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVTPWGIPTKGYKTRSNKRTQDLIIKRRNQK"
     gene            3027..3302
                     /gene="rpsS"
                     /locus_tag="PKI23_05095"
     CDS             3027..3302
                     /gene="rpsS"
                     /locus_tag="PKI23_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNN36350.1"
                     /translation="MPRSLKKGPFCDFHLFRKVEVAATKSDKRPIKTWSRRSMILPEM
                     VGLTIAVHNGKQHVPIFVSEDMVGHKLGEFAATRTFRGHVADKKAKR"
     gene            3327..3659
                     /gene="rplV"
                     /locus_tag="PKI23_05100"
     CDS             3327..3659
                     /gene="rplV"
                     /locus_tag="PKI23_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNN36351.1"
                     /translation="METMARLKMAGMSAQKARLVVDQIRGKRVDEALNLLAYSPKNSA
                     KVIRKVLSSAIANAEHNFGADIDRLKISTAYVDEGVTLKRIRPRAKGRADRISKRSCH
                     ISIKVSEI"
     gene            3669..4316
                     /gene="rpsC"
                     /locus_tag="PKI23_05105"
     CDS             3669..4316
                     /gene="rpsC"
                     /locus_tag="PKI23_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNN36352.1"
                     /translation="MGQKVNPTGIRLGITKGHNSVWYADRDRYQKTLLADVRAREYIF
                     KKLAGASISRIEIERPAQTAKITLYTARPGIVIGKKGEDVEKLKKEVFGILNVPVQIS
                     IEEIRKPDIESQLVADSIAQQLERRVMFRRAMKRAVQNALRQGAEGVKVQVSGRLGGT
                     DIARTEWYREGRVPLHTLRAEIDYATSTAKTTYGIIGVKVWIFKGEVLDRRERKE"
     gene            4380..4793
                     /gene="rplP"
                     /locus_tag="PKI23_05110"
     CDS             4380..4793
                     /gene="rplP"
                     /locus_tag="PKI23_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNN36353.1"
                     /translation="MLQPKRTKFRKQFKGRNRGLAHRGSEVSFGEFALKATSRGRITA
                     RQIESARRAMTRKVRRGGKIWIRVFPDKPITQKPLEVRQGKGKGSVEYWVALVQPGKI
                     LYEIEGVSEELANEAFTLAAAKLPVQTSFVKRALM"
     gene            4793..4993
                     /gene="rpmC"
                     /locus_tag="PKI23_05115"
     CDS             4793..4993
                     /gene="rpmC"
                     /locus_tag="PKI23_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNN36354.1"
                     /translation="MKYSELSGKSLQELKEMLLSARSSQFRYRMQKATSQLAQTHLLG
                     EVKADIARLKTAIRQKAGESNG"
     gene            4986..5255
                     /gene="rpsQ"
                     /locus_tag="PKI23_05120"
     CDS             4986..5255
                     /gene="rpsQ"
                     /locus_tag="PKI23_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNN36355.1"
                     /translation="MAEKHESVRTLAGKVVSDKMDKTITVLIERKVKHPVYGKYVRKS
                     TKVHAHDEKNECRIGDKVLIKASRPLSKTKFWELVNIESRAVQLD"
     gene            5286..5654
                     /gene="rplN"
                     /locus_tag="PKI23_05125"
     CDS             5286..5654
                     /gene="rplN"
                     /locus_tag="PKI23_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNN36356.1"
                     /translation="MIQVETVLDVADNSGARRVMCIKVLGGSHRRYAGVGDLIKVTVK
                     EAIPRGKVKKGQVMNAVVVRTRSGVRRSDGSIIRFDGNAAVLLNNQKQPIGTRIFGPV
                     TRELRSEQYMKIISLAPEVL"
     gene            5666..5983
                     /gene="rplX"
                     /locus_tag="PKI23_05130"
     CDS             5666..5983
                     /gene="rplX"
                     /locus_tag="PKI23_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNN36357.1"
                     /translation="MNKLRTNDEVIILAGKDKGKRGKILRVFPDDRILVSGVNLIKKH
                     QKPNPQIGVLGGIIEKEAPIHVSNASIFNASLGKADRVGFRYLEDGQKVRFFKSSNEV
                     IEK"
     gene            6000..6539
                     /gene="rplE"
                     /locus_tag="PKI23_05135"
     CDS             6000..6539
                     /gene="rplE"
                     /locus_tag="PKI23_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNN36358.1"
                     /translation="MPALKDKFKNEIMPKLKDEFGYMNPHEIPQIVKVTLNMGVGEAV
                     GDKKILDHALADMEKISGQKPVVTRAKKSVAGFKVREGWPIGCKVTLRRDRMYEFLER
                     LLQIALPRERDFRGLNPKSFDGRGNYSMGVKEQIIFPEIDYDKVDRVRGLDICITTTA
                     QTNKEALALLKAFNFPFRG"
     gene            6543..6848
                     /gene="rpsN"
                     /locus_tag="PKI23_05140"
     CDS             6543..6848
                     /gene="rpsN"
                     /locus_tag="PKI23_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNN36359.1"
                     /translation="MARLSVIQRELKRAATVEKYAEKRAALKEVILSPSSTDEQKWNA
                     QFALQKLPRDSSAVRKRNRCQLTGRPHGYYRKFGLARNKLREHAMKGDIPGLVKASW"
     gene            6871..7266
                     /gene="rpsH"
                     /locus_tag="PKI23_05145"
     CDS             6871..7266
                     /gene="rpsH"
                     /locus_tag="PKI23_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNN36360.1"
                     /translation="MSMQDPLADLLTRIRNAQMAGLKQVSISASKIKIDVVKVLKSEG
                     YIADYSCASDDLKPLLTINLKYHEGAGVISEIKRISRPGLRRYCASQDIPKVKDGLGV
                     AILTTSRGVMTDRDARKAGVGGEVICTVF"
     gene            7297..7824
                     /gene="rplF"
                     /locus_tag="PKI23_05150"
     CDS             7297..7824
                     /gene="rplF"
                     /locus_tag="PKI23_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNN36361.1"
                     /translation="MSRVAKKPISVPKGVEIKLENNVVSIVGPKGTLSTLLNSDIALE
                     QQSGSLALSVRDSRAWAIAGTTRALLNNMVSGVAQGFSKRLQLVGVGYRAKVTGDVLN
                     LSLGYSHPIDYKLPEGITAEVPSQTEIVLSGIDKRLLGQVAAEIRAFRPPEPYKGKGV
                     RYFNEQIHRKEAKKK"
     gene            7833..8180
                     /gene="rplR"
                     /locus_tag="PKI23_05155"
     CDS             7833..8180
                     /gene="rplR"
                     /locus_tag="PKI23_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNN36362.1"
                     /translation="MNKKALRLRRSMRTRAKIKENGVHRLCVHRTPRHIYAQIIQSGS
                     SNVVASASTCEKGVVEGVTGNSESAAKIGSLIAARALAAGVDKVAFDRSGFKYHGRVK
                     ALAEAARAGGLEF"
     gene            8192..8698
                     /gene="rpsE"
                     /locus_tag="PKI23_05160"
     CDS             8192..8698
                     /gene="rpsE"
                     /locus_tag="PKI23_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNN36363.1"
                     /translation="MALTEKVDANEGLLEKLVQVNRVAKVVKGGRIFSFTALTVVGDG
                     QRRVGFGRGKAREVPQAIQKAMEAARKNMVDVRLNGSTLFYPVKASHGAAKVYMQPAS
                     EGTGVIAGGAMRAVLELAGVQNVLAKCYGSTNPVNVVQATLNGLKQIQSPDMVANKRG
                     KSLEEIVG"
     gene            8703..8894
                     /gene="rpmD"
                     /locus_tag="PKI23_05165"
     CDS             8703..8894
                     /gene="rpmD"
                     /locus_tag="PKI23_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNN36364.1"
                     /translation="MNKSGKIRVTLKSSLHGRLKKHKACLKGLGLSRIGQTVEVLNTP
                     ENCGMIFKVNYLLRVEECE"
     gene            8895..9347
                     /gene="rplO"
                     /locus_tag="PKI23_05170"
     CDS             8895..9347
                     /gene="rplO"
                     /locus_tag="PKI23_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNN36365.1"
                     /translation="MGNLLSLNELSPGLGSKTGKKRVGRGIGSGLGKTCGRGHKGQRA
                     RKSGNVKAGFEGGQMPLQRRLPKFGFTSQISLRTAEVRLQELNALPQEVIEVDFSTLK
                     KFGIVRKDMKRAKLIGASGFDRAITLRGIMVSAGALAAIEAAGGRLDS"
     gene            9355..10650
                     /gene="secY"
                     /locus_tag="PKI23_05175"
     CDS             9355..10650
                     /gene="secY"
                     /locus_tag="PKI23_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNN36366.1"
                     /translation="MAQVPQKAPAGFNELINRLGFLLLAIIVYRIGTHIPVPGINPNR
                     LAALFEKSQGTILGLFNMFSGGALERMSILALGIMPYISASIIMQLLSTITPQLEQLK
                     KEGEAGRRKITQYTRYLTLVLASIQAVGMSLGLAGQNLAFASNLSFHFVASVSLITGS
                     IFLMWLGEQITERGIGNGISMLIFAGIAAGFPSAIGQAIEQTRQGELNIFVLFVIALV
                     ALVVVAFVVFIERGQRRITINYAKRQQGRTSYAAQVSHLPLKINMSGVIPAIFASSIL
                     LFPASIAQWLGQSNGFSWLQEVAMMLSPGQPLNIILFAVLIIFFSFFYTAMMFNPREV
                     AENLKRSGALIPGIRPGDMSARYIDGVLMRLTLFGAIYIAAVCLLPQFLVVLFNVPFY
                     LGGTSLLIVVVVVMDFVAQIQSHLMSHRYESLMKKSARK"
     gene            10700..10816
                     /gene="rpmJ"
                     /locus_tag="PKI23_05180"
     CDS             10700..10816
                     /gene="rpmJ"
                     /locus_tag="PKI23_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNN36367.1"
                     /translation="MKVRASVKKVCRNCKVVKRKGVVRIICAVEPRHKQRQG"
     gene            10846..11202
                     /gene="rpsM"
                     /locus_tag="PKI23_05185"
     CDS             10846..11202
                     /gene="rpsM"
                     /locus_tag="PKI23_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNN36368.1"
                     /translation="MARIAGINIPDNKHAVISLTYIYGIGKTTALKICANSGIDPAKK
                     IQDLSGEQLDVIRNEVAKFVVEGDLRRETSMSIKRLMDLGCYRGLRHRRGLPLRGQRT
                     RTNARTRKGPRKPIRK"
     gene            11231..11620
                     /gene="rpsK"
                     /locus_tag="PKI23_05190"
     CDS             11231..11620
                     /gene="rpsK"
                     /locus_tag="PKI23_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNN36369.1"
                     /translation="MAKNDSSSRKKAKRNVTEGVAHIFASFNNTIVTITDRQGNTLSW
                     ATSGGSGFRGSRKSTPFAAQVASERAATAAQEYGLKNVNVEVKGPGPGRESAVRALHN
                     SGLKVGSITDVTPIPHNGCRPPKRRRV"
     gene            11671..12291
                     /gene="rpsD"
                     /locus_tag="PKI23_05195"
     CDS             11671..12291
                     /gene="rpsD"
                     /locus_tag="PKI23_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNN36370.1"
                     /translation="MARYIGSTCKLARREGSDLSLKSGVRPLESKCKIAALPGQHGQQ
                     KGRLSDYGVQLREKQKVRRIYGVLEKQFKNYYLKAARLKGATGENLLRLLESRLDNVV
                     YRMGFASTRAEARQLVSHKSILVDGKVVNIPSFQVEAGSSIEVREKAKGQLRIRSALE
                     LSEGRKSCDWISVDMTKLQGVFTRFPDRQDLPSEINENLIVELYSR"
     gene            12318..13307
                     /gene="rpoA"
                     /locus_tag="PKI23_05200"
     CDS             12318..13307
                     /gene="rpoA"
                     /locus_tag="PKI23_05200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNN36371.1"
                     /translation="MQNFTADLLAPGSIEVQEISRHRAKIILEPLESGFGHTLGNALR
                     RILLSSMPGAAISHVEITGVDHAYSTIEGVREDVVEIMLNLKNVAIRMDGRSRALCTL
                     TKQGPGTITAGDIKLSNDMEVANPDFVIAHIVDDSEVKMSLEVSKGTGYVTSDSTRAL
                     DSDTVPIGRLYLDASFSPIRRVAYSVESARVEQRTNLDKLIIEIETNGTIGAEEAVRG
                     AASILQQQLAVFIDLRDEIKREPSRHKSQVDPILLRPVDELELTVRSANCLKAENIHY
                     IGDLIQRTEVELLKTPNLGKKSLTEIKDVLAVHGLSLGMRLDDWPPYELKKPD"
     gene            13360..13725
                     /gene="rplQ"
                     /locus_tag="PKI23_05205"
     CDS             13360..13725
                     /gene="rplQ"
                     /locus_tag="PKI23_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNN36372.1"
                     /translation="MRHRNSGRSFSRTSAHRKVMFRNMAASLFEHELIKTTLPKAKEL
                     RSFAEPLITLAKVDTVANRRLAFDRVRDKKAVGKLFLTLGPRYKNRPGGYLRVLKCDY
                     RAGDNAPMAYVELVDRPEE"
     gene            complement(13904..15697)
                     /locus_tag="PKI23_05210"
     CDS             complement(13904..15697)
                     /locus_tag="PKI23_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNN36373.1"
                     /translation="MHSATADAPQPLNFADLGLPEPIQRALDDLGYESPSPIQARTIP
                     FLLAGRDLLGQAQTGTGKTAAFALPLLTQIDLSRPQLQGLVLAPTRELALQVSEAFRS
                     YAVHLPGFRICTVYGGQGYREQLIQLRQGVHVVVGTPGRVMDHMRRGTLDLGTINHLV
                     LDEADEMLRMGFIDDVEWILEQTPPKRQVALFSATMPVPIRRIARQHLHDAEEVAIKS
                     QTTTVEAIRQRFWRVGGVDKFEALSRILETIDHDAIIIFVRTKTETLALAEQLNARGH
                     TAAPLNGDIPQSLREQTIARLKSGETAILVATDVAARGLDIDRVTHVINYDIPYDPEA
                     YVHRIGRTGRAGRSGEAILFVTQRENRLLHAIEQTTGHKIERMYLPTVAEVNQKRIER
                     FHAHISSALDTFDLTLYQKLLQEYQTQSQRPLIDIASALAAMLIGGELLLPQPSEEEE
                     QLQQRSRDGGRTREREAPSVRRATSDTGPKRGKMTAPVDASLENYRVEVGRNQGLKPG
                     NLVGAIANEAGIDSKYIGRIEIFDDYSLVGLPQGMPKELLHLLKKVWVAGKQLKISRY
                     EQPTTTKGAAKTKYPPFAEKQKGRPAGSKNR"
     gene            complement(15848..16375)
                     /locus_tag="PKI23_05215"
     CDS             complement(15848..16375)
                     /locus_tag="PKI23_05215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNN36374.1"
                     /translation="MPRSPARGGRSRDEIDASGYRPNVGIILANPQGQLFWARRIGRQ
                     EAWQFPQGGIQRGESPEQALFRELHEEVGLEPHDVRILACTCGWLHYQLPKRLVRNQP
                     DSHFVGQKQKWFLLMMQGEESRVRVDHSRHPEFSQWRWVSYWHPLSRVVEFKREVYRR
                     ALHELSLPLAEHLAG"
     gene            16468..17121
                     /locus_tag="PKI23_05220"
     CDS             16468..17121
                     /locus_tag="PKI23_05220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNN36375.1"
                     /translation="MPLAIFDLDNTLLGGDSDHAWGEFLVSEGWVDAERFRASNDLFY
                     QRYLNGDLDIFEYQAFALAPLKEMGRAMAEQLRTRFLTERIAPLILPKGLALLEQHRR
                     RGDFLLIITATSHFITEPIASQLAVDDLIATMPELADGEFTGRVAGVPSFREGKVARL
                     RQWLDERALSLDGSSFYSDSHNDLPLLSLVENPVAVDPDPLLEAHARGASWPVISLR"
     gene            complement(17125..18318)
                     /locus_tag="PKI23_05225"
     CDS             complement(17125..18318)
                     /locus_tag="PKI23_05225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNN36376.1"
                     /translation="MTSTLPRLLLKSGHDRRIKKGHRWVYSNEVDTNRSPLTQFAPGT
                     LATVTSAQGKPLGSALVNPNTLIAARLYSREGDVVIDEEMLTRRLEAALRLRRQLFAS
                     DHHRWVYGDSDALPGLVLDCYGEVVVGQISSAGMEQLKPMLETAIAAVYAPATLIWRN
                     QGGFRAIEGLDEYTEVASGELPQQLEISENGGRFVAPAQGGQKTGWFYDHRLNRARLG
                     IHARARRVLDLYSYVGGWGIQAALAGASSVTCVDSSAHALEWVNENARRNGVAERVST
                     EQGDAIDFLKACRDSRRQFDLILLDPPSLIPRRKFHTQGLKSYHQLNQLALKLLSDEG
                     ILITSSCSSHLQPAELWEVVVSAARHDGRLAQVLEQGHQGPDHPIHPAMPETQYLKSL
                     TVRAC"
     gene            complement(18351..19838)
                     /gene="gcvPB"
                     /locus_tag="PKI23_05230"
     CDS             complement(18351..19838)
                     /gene="gcvPB"
                     /locus_tag="PKI23_05230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNN36377.1"
                     /translation="MLIFDQSRPQRAAHAQAPDRPADCSDLPTELLRATPPPLPELSE
                     LQVVRHYTRLSQRNFSIDTHFYPLGSCTMKYNPRGAHKAASLPGFLNRHPLAPISLSQ
                     GFLGALHRLQEILQSVTGMAGVSLTPMAGAQGEFAGVAMIRAYHQARADHARSEILIP
                     AAAHGTNPATAAMCGYGVREIPLKPDGDVDLTALRAALGPHTAGLMMTNPSTCGLFER
                     GISEIADAVHGAGGLLYYDGANLNAILGRAKPGAMGFDVLHLNLHKTFATPHGGGGPG
                     AGPVAVNERLLPYLPTPYVGKVVDADGQPRFGWIDQAQAPASIGHLSSFMGNAGILLR
                     ALAYALMLGREGMARVSDHATLNANYLAQRLVEIGYELAYPQRRATHEFIITLQQSAK
                     ALGITAMDVAKRLLDHGYHAPTTYFPLIVPEALLIEPTETESKETLDGFIDAMAKILT
                     EATHDPERLRQAPHSLPVRRLDDTRASRNPDLVWRPDANLNRNPS"
     gene            complement(19868..21217)
                     /gene="gcvPA"
                     /locus_tag="PKI23_05235"
     CDS             complement(19868..21217)
                     /gene="gcvPA"
                     /locus_tag="PKI23_05235"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNN36378.1"
                     /translation="MPFIPHTEAEVTQMLATIGVDRIDALFDEIPPHLHDSSLDGLDA
                     GLSEWEMLAELRTLAAQDDNVLCFAGAGCYAHHIPAAVWDIIGRGEFMTAYTPYQAEA
                     SQGTLQLLYEYQSMMATLMAMEVSNASLYDGASALAEAILMALRANRSASARRVWVAG
                     TIHPLYLRAARDIVRNQQIELDLLPLDASGVVDLKALPAASDDHAALVIPQPNYLGRL
                     EAVDQLTDWAHANGLLAIALVNPLAMALLKPPGQWGSRGADIAVGDGQPLGIPMASGG
                     PSFGFVCCREALVRQLPGRLIGRTLDRDGRVGFTLVLQAREQHIRRAKATSNICTNQG
                     LLVAAATLYLSLMGPGLARIAARCHDNCRTLAERLRAIPGVELPLDGPCFHELVVRLP
                     RDPRQTLARLADERIFGGVPLADDFAQWSDCMLVCTTETSSAADIDRYIAALERALG"
BASE COUNT         4672 a         5190 c         6698 g         4724 t
ORIGIN      
        1 cgaaggcggc cgcaccgtcg gcgccggcgt cgtctccaaa gtgattgcct gagcgaggga
       61 ttccaagcgg attgtgccgg catcctttct tgacagcagg agcggccatc tataacatgc
      121 tgcttccttt tttggccggc atggattcgg tgtctgatag acagtgtagg accaaatgca
      181 aaatcaacga atacgcatac gtctcaaagc ttttgatcat cgcctgatcg atgtttcagc
      241 acaagagatc gtggaaactg ccaagcgcac tggtgcgcag gtcaaggggc cgattccgct
      301 gccgactcgc aaggagcgtt tcacggtgct gatttcgccg cacgtgaata aggatgcgcg
      361 cgatcagtat gagatccgca cttacaaaag actgctggac atcgtcgatc cgacagacaa
      421 gacggtcgat gcattgatga agctcgatct cgctgcaggg gttgatgttc agatcagtct
      481 cggctgagtt ggtctgctga tggttaatca tcgcgcaaat cgcgatccca tgaataatga
      541 tgtgtagcac tttgaaataa gtggccatag cgggtgagag ccccatgcac aggagagagt
      601 ctgatgtcga ttggtttagt ggggcggaag agcggcatga ccaggctttt tacgcctgcg
      661 ggtgaatcga ttccggtcac ggttttggag gtttcgccca atcgcgtggt tcaggtcaag
      721 agtgccgcgc gtgatgggta ttgtgccgtt caggtcacga cgggttcgag aaaagcgtcg
      781 cgggttgcaa aggctgaagc gggtcatttc gccaaggcga atgtcgaggc ggggcgcggg
      841 ttgtgggagt tcgtgattga gggtgagtcg gagttggtgc ctggcggcga gctcgatctt
      901 tcgatctttg aggtcggcca gcatgtggat gtgactggag tgtcgaaagg caagggcttt
      961 gcgggtgtgg tgaagcgttg gaattttcgt acgcaggatg cgacgcatgg caattcgctg
     1021 tcgcaccgcg cgcctggctc gattggtcag aaccagacgc caggtcgggt ctggaaagga
     1081 aaaaagatgg ctgggcacat gggggccgag caggtcaccg cgctcaatct ggaaattgtc
     1141 aagatcgatc tcgagaagaa tgcgctgctc gtgcggggtg ccgtaccggg agcggcaggt
     1201 ggtgatttga tcgtcagacc atctgtgaag tcaaagtgag gccgatcatg gagcttgaag
     1261 ttaaatcctt tgtcgatggc gaaattcctt ctggatcgat ttcgctttct gatcaggtct
     1321 tcggtcagga gttcaacgag tcgctggttc atcagattgt cgtggcctac ttggcgggtg
     1381 gtcggcaggg ctcgaagggg cagaagacgc gcgccgaggt gcgtggtggc gggaaaaaac
     1441 cgtggcgaca aaagggttca gggcgtgcgc gcgctggatc gagccgcagc ccgctgtggc
     1501 gatcgggtgg taaaagcttt gcggccactc cgcgtgatca tgggcagaag gtcaacagaa
     1561 agatgtaccg ggcagcgctt cgctcgattt tttctgagct ggttcggcag gagcgggtca
     1621 ctgtgttggc tgacttctct cttgagtcgc ccaagaccaa gaatctcctg gagaaactga
     1681 gtggaatcca ggagcagaag atcctgatcg ttacgctgga gtttgagaac aatctctatc
     1741 tggcctcacg aaacttgatc ggggttgatg tccttgaggc cgattcggtc gatccggttt
     1801 ccttggtcgg aagcgaaagg attctagtga ctctttctgc attgaagaaa attgaagagg
     1861 cgttggcatg aatcaggagc gcatttacag catactgctg agtcctcatg tctccgagaa
     1921 agcagccgga gtagctggga aatacaacca gtatgtcttc cgggttgcaa aaaactcgaa
     1981 caaaagagag attcacaagg cggtcgagac gcttttcaag gtaaaagttt cttctgttca
     2041 aacgctcaat gtaaaaggca aagtcaaaag aaaccggcat gggctggtta acaggccgga
     2101 ctggaagaaa gcgtatgtgc ttcttgagcc cggcaatgac atcgacttca cttcgatagg
     2161 ctgatctggg gatttctgat ggcagttttg agtaaaaaac cgacttcgcc ggggcgaaga
     2221 ttccagatca gtgtcaccaa tccagaattg cacaagggtg cgccgtgcgc ggcgcttctg
     2281 gaaaagaagc gcaagaatgg cgggcgaaac aacgctggga ggatcaccgt tcggcatcag
     2341 ggcggcggac acaagcaaca ctatcgagtg atcgactttc gccgcgacaa ggattccatt
     2401 ccatctgtcg ttgagcgaat cgagtatgat ccaaaccgca gtgcccacat cgcgctgctt
     2461 aaataccgcg acggcgagcg gcgctacatc gttgcgccaa aagggttgaa agccggcgat
     2521 gaggttgttt caggtccatt agcgccgatc aaggcgggta atgcattggc gctcagaagc
     2581 atcccgttgg gaagcctgat tcactgtgtt gaactgaaac ctggaaaggg tgcgcaattg
     2641 gcccgaagtg ccggtgcgtc gatccagttg gtggcgaaag agggtgttta tgcgaccctt
     2701 cgccttaaat ctggagagat gcgcaaggtt tcgatcgagt gtcgggccag cattggcgag
     2761 gtttctaaca gcgagcacaa cctgagatcg ctgggtaaag cgggcgcgtc gcgctggcgt
     2821 ggtcgcaggc ccacagtgcg tggtgtggcg atgaacccgg ttgaccatcc gcatggaggt
     2881 ggagagggta gaacatccgg tggtcggcat ccggtaacgc cgtggggtat tccgaccaag
     2941 ggttacaaga cgcggtcaaa caagcgcacc caagacctta tcatcaagcg tagaaatcag
     3001 aagtaattga attttcggag attcttgtgc ctcgctcatt aaaaaaaggt ccattttgcg
     3061 attttcatct tttcagaaag gtggaagttg cagcgacaaa aagtgacaag cggccaatta
     3121 agacgtggtc gaggcggtcg atgattcttc cggaaatggt tggtttgacc attgcggttc
     3181 acaatggcaa gcagcatgtt ccgatctttg tttcggaaga catggttggg cacaagctgg
     3241 gagagtttgc ggcaaccaga accttccgcg ggcatgtggc ggacaagaag gccaagcgct
     3301 aacagcggtt ttagagagga tttacgatgg agacgatggc gcggctgaag atggcgggga
     3361 tgtctgccca gaaagccaga ttggtcgtgg atcagattcg aggtaagcgt gttgatgagg
     3421 cgctgaatct tctggcctac agtcctaaaa attctgcaaa agtgatcaga aaggttctga
     3481 gttcagcgat tgccaatgcg gagcataatt ttggcgcgga tatcgatagg ctcaaaattt
     3541 caactgcata tgttgatgag ggtgtaacgc tcaagagaat acggccgcgt gccaagggcc
     3601 gcgctgaccg catttccaag cgcagctgcc acatctccat taaagtgtcc gaaatctagg
     3661 agctgatcat gggtcagaaa gtcaacccca ccggaattcg cctgggcatt accaaggggc
     3721 acaattcagt ctggtatgcg gaccgtgatc gttatcagaa aaccttgctc gctgatgtcc
     3781 gggcacggga gtatatattc aagaagctcg cgggagcatc gatcagccgc atagaaatcg
     3841 agcgcccggc gcagacggcc aaaattacgc tctatacggc gcgcccagga attgtgatcg
     3901 gaaagaaagg cgaagatgtc gaaaaactga agaaagaagt cttcggcatt ctgaatgttc
     3961 cggtgcagat cagcattgag gagatccgaa aaccggatat cgaatcgcag ctggttgcag
     4021 acagtattgc gcagcagctg gagcgcaggg tgatgttccg tcgtgccatg aagcgagcgg
     4081 ttcaaaatgc acttcgccag ggggctgaag gggtcaaagt gcaggtttcc ggtcgccttg
     4141 gtggtactga cattgcgcgg accgaatggt atcgggaggg gcgcgtaccc ttgcacacat
     4201 tgcgtgcaga gattgattac gccacatcga ccgccaagac cacttatgga atcatcggcg
     4261 tcaaggtgtg gattttcaag ggcgaagttt tggatcgccg cgagcgcaaa gagtaaggtg
     4321 cgcaggttaa acttgaagga aattcgatct aaattgcttc gaagataaaa ggccaaacaa
     4381 tgcttcagcc taaaagaacc aaattcagaa aacagtttaa aggacgcaat cgcggtctgg
     4441 cgcatcgcgg cagcgaggta agctttggtg agttcgcgct gaaagcgacg tcaagaggtc
     4501 ggattactgc ccgtcaaatt gaatcagcga gacgcgccat gacgcgcaag gtccgtcgtg
     4561 gtggcaagat ctggatacgg gttttcccgg acaaaccgat cactcagaag cccttggaag
     4621 tccggcaggg caaaggcaag ggttcggtcg agtattgggt tgctctggtt cagccaggta
     4681 aaatactcta tgaaatcgaa ggggtctccg aagagcttgc caatgaagca tttacgcttg
     4741 cagcagcaaa gcttccggta cagacttctt ttgtcaagcg ggcattgatg taatgaaata
     4801 cagtgagctt tcaggcaaat ctctccagga gctgaaagag atgttgcttt cggcgcgaag
     4861 cagtcagttt cgttaccgga tgcaaaaggc gacttcacag cttgcacaaa cacacctgct
     4921 tggtgaagtg aaagcggata tcgccagatt gaagacggca atcaggcaga aagcgggtga
     4981 gagcaatggc tgaaaaacac gaaagtgtga ggacgctggc aggcaaggtt gtcagcgata
     5041 aaatggacaa gaccataacc gttctgatcg aaagaaaggt caagcacccg gtttatggca
     5101 aatatgtgcg caaatcgacc aaggtccatg cgcatgatga aaaaaatgag tgccgtattg
     5161 gggataaagt gctcatcaag gcttccagac cactgtcgaa gacaaagttt tgggaattgg
     5221 taaacattga gagtcgagcg gtacagcttg attgaagctg gcaggcgact ttctgggagc
     5281 gctaaatgat tcaggttgaa actgttttgg atgtcgccga caacagtgga gcgcgacgag
     5341 tgatgtgcat caaggttctt ggcggctctc accgccgcta tgcgggtgtc ggcgacctga
     5401 tcaaagtcac ggtcaaagag gctattccgc gtggcaaggt gaaaaaaggg caggtgatga
     5461 acgcggtcgt ggtcaggacc aggagtggcg tgcgccgttc tgatggttcg atcatccgtt
     5521 ttgatggcaa tgcagccgtg ctgctgaaca atcagaagca gccgattggg acccgcatct
     5581 ttggtccggt caccagagag ctgcgctctg aacagtatat gaagatcatt tctctggccc
     5641 cggaagttct ataggaaagg ttgagatgaa caaacttcgc acgaatgatg aggtcatcat
     5701 ccttgctggt aaggacaaag gaaagcgcgg gaagattctc agagtttttc cagatgacag
     5761 aatacttgtc tctggggtca atctgatcaa gaagcaccag aagcccaatc cgcaaattgg
     5821 cgtccttggt ggaatcattg agaaagaggc tccaatacac gtttccaatg cctcgatctt
     5881 caatgcgtca ctggggaagg ctgatagagt tggattcagg tatcttgaag atggccagaa
     5941 ggttcgcttc ttcaagtcaa gcaatgaagt gatagaaaag taattctttg gtgggccaaa
     6001 tgccagcgct gaaagataaa ttcaagaatg agataatgcc gaagctcaag gacgagttcg
     6061 gttacatgaa tccacatgaa attccccaga ttgtcaaagt gactttgaac atgggggttg
     6121 gagaagcggt tggtgacaag aagattcttg atcatgctct tgctgacatg gagaagatca
     6181 gtggacagaa gccggttgtg acccgtgcca agaagtcggt cgctggattc aaggtcagag
     6241 aaggttggcc gattggttgc aaggtcacgc ttagaaggga tcggatgtat gagttccttg
     6301 agcgtctgtt gcagattgcg ctgccgcgtg agagagattt cagggggctg aacccaaaat
     6361 ccttcgatgg gcgtggaaac tactcgatgg gggtcaagga gcagataatt ttcccggaga
     6421 tcgactacga caaggtcgat cgcgtcagag gtcttgatat ctgcattaca acaacggcgc
     6481 agaccaataa agaagcgctt gccttgttga aagcttttaa ttttcccttc agggggtgag
     6541 tgatggcacg gctttcggtg attcaacgcg agctgaaaag agccgcgact gtcgagaagt
     6601 atgcagagaa gcgagctgct ttgaaggagg taatactgag cccttcatcg acagatgagc
     6661 agaagtggaa tgcgcaattc gcgcttcaga aacttccgag agactcgagc gcggttagga
     6721 agcggaatcg ctgccaattg actggacgtc cccatggcta ttaccggaaa tttggcctgg
     6781 ccagaaacaa acttcgtgag catgccatga aaggtgatat tcctggcctg gttaaggcca
     6841 gttggtaagg tcgcactgga gagaaaattt atgagcatgc aagatccatt ggccgacctt
     6901 ttgactcgaa tcagaaatgc gcagatggca ggactgaagc aagtctctat atcggcgtcg
     6961 aagataaaga ttgatgtggt gaaagttttg aaaagcgaag gctacatcgc tgactattcc
     7021 tgcgcgtcgg atgatctaaa accgctcctg acaataaatt tgaagtatca cgagggtgca
     7081 ggagtcatct cggaaatcaa acgaatcagc cgtcctggac tgagaagata ttgcgcgagc
     7141 caggatattc cgaaagtaaa agatggtctg ggcgttgcca tactgacgac cagtcgtggg
     7201 gtgatgactg atagagatgc gagaaaagca ggtgtgggcg gagaggttat ctgcaccgtt
     7261 ttttgatgtt ttacgaatgg ttgctgggac aaagttatgt ctagggttgc aaaaaagccg
     7321 atctctgttc ctaaaggcgt ggagataaag ctggaaaata atgtcgtttc gattgtgggt
     7381 ccgaagggaa cgctttcgac gctgttgaat tcggatattg cgctggagca gcaatcggga
     7441 tcactggctc tttcagtgag agacagtcgt gcgtgggcga tcgcaggaac gacgcgtgcc
     7501 ctgttgaata atatggtttc cggggtcgct caaggatttt cgaagagatt gcagctggtt
     7561 ggtgtgggtt accgggctaa ggtgactggc gatgtattga atctttcgct tggctattca
     7621 catccgattg attacaagct tccagagggc attaccgcag aggtgccgtc ccagactgaa
     7681 atcgttctgt cagggatcga caagcgtttg cttggccagg ttgctgcgga aatccgcgcc
     7741 ttccgtccgc cagagccata caaaggcaaa ggggttcggt atttcaatga gcagattcat
     7801 cgtaaagaag ccaaaaagaa gtgattttca tgatcaacaa aaaagcatta cgcttgcggc
     7861 gctcgatgcg gaccagagcg aaaatcaaag agaatggcgt gcaccgtctg tgcgtacatc
     7921 gtactccaag acatatctac gcacagatca ttcagtcagg aagttcgaat gtcgtggcct
     7981 ctgcctcaac ttgtgaaaag ggtgttgttg agggcgtgac tggcaacagc gagtcggccg
     8041 cgaagatcgg ttccttgatc gcagcccggg cattggccgc cggggtggat aaagtggcat
     8101 tcgatcgttc cggttttaaa tatcatggca gagtcaaggc cctggcggaa gccgcccgcg
     8161 ctggcggttt ggaattttag aggaaaagat catggcgctg actgaaaaag ttgatgcgaa
     8221 cgaagggctt cttgagaagc tcgttcaagt aaacagagtt gccaaggttg tcaaaggcgg
     8281 gcgtatattc tcttttaccg cgctgactgt tgtgggtgat ggtcagcggc gtgttggctt
     8341 cggtcgcgga aaagcccggg aggttccgca ggcgatacag aaagccatgg aagcagcacg
     8401 aaagaacatg gttgatgtgc gtctgaatgg ctcgaccctg ttttacccag tcaaggccag
     8461 tcatggtgcg gccaaggtct acatgcagcc ggcatcggaa ggcaccggag tcattgcggg
     8521 cggtgccatg cgtgctgttc ttgagctggc gggcgtacag aatgttcttg ccaagtgtta
     8581 tggatcaacc aaccctgtca atgtcgtgca ggcaactctc aatgggctca agcagattca
     8641 atctcccgac atggttgcga ataaacgcgg aaagagtttg gaagagatcg ttgggtaact
     8701 atatgaacaa gtctggaaaa atcagagtaa cgcttaagtc aagtttgcat ggcagactga
     8761 aaaagcacaa agcttgtctc aaggggctgg gcctgagtcg tatcggtcaa actgtcgaag
     8821 tgctgaatac cccagaaaat tgcgggatga ttttcaaggt caattatctg ttgcgcgtcg
     8881 aggagtgtga ataaatgggc aatttactct ccctgaatga gctttctccc gggttgggtt
     8941 ccaagactgg aaaaaaacgg gtcggccgcg gcattggtag cgggcttgga aagacatgcg
     9001 gtcgcggaca caaggggcag cgggcacgca aaagtggcaa tgtcaaagcc gggtttgaag
     9061 gtggtcagat gccgctccag cggcggttac cgaaatttgg cttcacttcg cagatctctc
     9121 tgagaacggc tgaggttcgg ctccaggaat tgaacgctct ccctcaagag gtgatcgaag
     9181 tcgatttttc gacgctgaag aaattcggca tcgtcagaaa agacatgaag cgtgccaaat
     9241 tgatcggtgc ctctggattc gatcgcgcca ttactctgcg aggtataatg gtcagcgccg
     9301 gtgcgcttgc tgcgattgaa gccgctggcg gaagacttga ttcgtaatcg aacgatggcg
     9361 caagttccac aaaaagctcc agctggattc aatgagctca tcaaccggct gggctttctt
     9421 ctgttggcga ttattgtcta tcggattgga acacatatac ctgttcctgg catcaacccg
     9481 aatcggttgg ctgcactgtt tgagaagagc cagggaacga ttctcggtct tttcaacatg
     9541 ttttcaggtg gtgcgcttga gcggatgagc attcttgcgc ttgggatcat gccgtacata
     9601 tcggcatcga tcatcatgca gttgctgtcg accatcactc ctcagcttga gcagttgaaa
     9661 aaggagggag aggctggtcg ccggaagata acgcaatata cccgctatct gacgcttgtg
     9721 ctggcatcca tccaggcggt aggcatgtct ctcgggctgg cggggcagaa tttggctttt
     9781 gcttcaaact tgagtttcca tttcgtggcg tcagtctcct tgatcacggg ctcaatcttt
     9841 ttaatgtggc ttggcgaaca gattactgag agaggcattg gaaacggcat ctccatgctc
     9901 atttttgctg gaatcgctgc aggttttcct tctgcaattg gtcaggcgat cgagcagact
     9961 cggcagggtg agttgaatat atttgttctt tttgtgattg cactggttgc attggtggtc
    10021 gtggcctttg tggtcttcat tgagcgtggt cagcgacgta tcacgatcaa ctatgcaaag
    10081 cgacagcaag gtcggacaag ctatgccgcg caagtttctc atctgccact caagatcaac
    10141 atgtcaggtg tgatacctgc cattttcgcc agcagcattc ttctgtttcc tgcctcgatt
    10201 gcgcaatggt taggacagtc caatggcttc tcctggctgc aggaggttgc gatgatgctc
    10261 agccccgggc aaccgctgaa tatcattctt tttgcggttt tgataatatt tttcagcttc
    10321 ttctacacgg ctatgatgtt caaccctcgt gaagtggcgg aaaatctgaa acgatctggc
    10381 gctttgatcc cggggattcg acctggggat atgtctgcgc gctacatcga tggagtgttg
    10441 atgaggttga cgctttttgg cgcgatttat attgccgctg tctgtctgtt gccgcagttt
    10501 ctggttgttt tgtttaatgt gccgttctac cttggtggaa catcgctgct gatcgttgtg
    10561 gttgtcgtga tggactttgt ggcgcaaatc cagtcgcatc taatgagtca tcgttatgaa
    10621 tcgttgatga agaagtcggc cagaaaatga taaaggatcg aatttatact ctttacgtga
    10681 tcatgagcat ggggttgtta tgaaagtcag agcgtctgtc aaaaaagttt gccgaaattg
    10741 caaagtggtg aagcgcaaag gcgtggtccg gatcatttgt gcagtcgagc cacggcataa
    10801 gcaaaggcaa ggctgattca attaatttgt cattggagca tctgaatggc tcgtattgcg
    10861 ggaattaaca ttccagacaa caagcatgct gtcatatctc tgacttacat ctacgggatt
    10921 ggcaagacca ctgcgctcaa gatatgtgcg aattctggaa ttgatcctgc gaaaaagatt
    10981 caggatctct cgggagagca acttgatgtc atccgaaatg aagtcgccaa gtttgttgtg
    11041 gagggtgacc tcagacgtga gacgtcgatg agcatcaagc gattgatgga tcttggctgc
    11101 taccgcggat tgcgccatcg tcgtggtttg ccgttgagag gccagcgtac ccgtaccaat
    11161 gcgcgaaccc gtaaaggccc gcgcaagccg atccgtaagt aatttttttc tacagatttg
    11221 gacagaatcc atggctaaga acgatagcag ttcaagaaag aaggcgaaaa ggaatgtcac
    11281 tgaaggggtt gcgcacattt ttgcctcctt caacaatacc atcgtcacga tcactgaccg
    11341 tcaaggaaac acattgtcat gggccacctc gggtggttct ggctttcgtg gttcaagaaa
    11401 gagcacaccc tttgcggctc aggttgcctc ggagcgcgct gccacggctg cccaggagta
    11461 tggcctgaag aatgtgaatg tcgaagtaaa gggcccgggc cctggaagag agtcggcggt
    11521 gcgtgcactg cacaacagtg ggctgaaagt tggcagtatt actgatgtta caccgattcc
    11581 gcacaacggt tgccgtcctc cgaaacggcg ccgtgtctga ttggattgta gtcacaccac
    11641 tggctggatt aacagataga gagttgataa atggctaggt atattggatc tacctgtaag
    11701 ttggcgcggc gtgaagggtc agatctttcg cttaagagtg gcgtccgacc ccttgagtca
    11761 aaatgcaaga ttgcggcttt gccagggcag catgggcagc agaaaggcag gctttctgat
    11821 tatggagtcc agcttcgaga aaagcagaag gttcgccgaa tctacggagt acttgagaag
    11881 caattcaaga actactacct gaaggcagcc cgtttgaaag gcgccaccgg tgagaatttg
    11941 ctcagattgt tggagtcgcg tcttgacaat gtggtgtatc gcatggggtt tgcttcaacg
    12001 agagcggaag cacgccaact ggtgagccac aaatccattc tggttgatgg aaaggttgta
    12061 aacattccct cgtttcaggt tgaggccggt tcttcaattg aagttcgtga aaaagcgaaa
    12121 gggcagttgc ggatccgttc tgcgcttgaa ttgtctgaag gccgaaagag ctgtgattgg
    12181 atttcggtgg acatgacaaa gctgcaaggt gtcttcacgc gattcccgga tcgacaggat
    12241 ctgccatccg agatcaatga aaatttgatt gtcgagctgt attcgcgatg attttgtttg
    12301 gttacaggta cctatagatg caaaatttca cagctgatct tttggctccc ggatctattg
    12361 aagtccagga aatatcgcgt cacagagcca agatcattct ggaaccgttg gaatctggct
    12421 ttggtcatac gctgggcaat gcattgcggc gcattctgct ctcatcgatg cctggtgctg
    12481 ccattagcca tgttgaaatc actggtgtcg atcacgctta cagcaccatc gagggtgtac
    12541 gggaagatgt ggtcgagatc atgctcaact tgaaaaatgt tgcaattcgc atggatgggc
    12601 gttcgcgcgc actgtgcact ttgaccaagc aaggtccagg gaccattacc gcaggcgaca
    12661 tcaagctgtc taatgacatg gaggttgcca atcctgattt cgtcattgca cacatcgttg
    12721 acgattcaga agtgaaaatg tcactggagg tttcgaaggg cactggctac gtgaccagcg
    12781 actctacacg ggctcttgac agcgatacgg tgccgattgg cagactttat ctggatgcaa
    12841 gcttcagccc gatcagaagg gttgcttaca gcgttgagtc ggcccgagtc gagcagcgca
    12901 caaacctgga taaactgatc attgaaattg aaaccaacgg caccattggt gctgaagagg
    12961 cagtacgtgg tgctgcatca attctccaac agcagttggc agtcttcatt gatttgagag
    13021 atgagatcaa gcgtgagcca agccgccaca agagccaggt tgatccgatt cttttgcggc
    13081 ccgtcgatga gctggagctg acggtacgtt ctgccaactg tctgaaagca gagaatatcc
    13141 actatatcgg cgatctgatc cagcgcacag aagtcgaatt gctgaagacg ccaaacctgg
    13201 gcaaaaaatc gctgaccgaa atcaaggatg tgctggcggt tcatggattg tcgttgggta
    13261 tgcggttgga tgattggcct ccttacgagt tgaagaagcc ggattgattt ctgttttttg
    13321 cgattattca tacgaagtga taggtgggag attctattta tgcgccatag aaacagtggg
    13381 cgttcattca gtcgaaccag tgcgcatcgc aaggtcatgt tccggaacat ggcggcctcc
    13441 ctttttgagc atgaattgat caagaccact ttgcccaagg cgaaggaact gcgcagtttt
    13501 gcggagccgt tgatcacttt ggccaaagtg gatacggttg ccaacagaag gttggcgttt
    13561 gatcgggtca gggataaaaa ggcggtcggt aagctgtttt tgaccttggg tccacgctat
    13621 aaaaaccgcc cagggggtta tttgcgtgtt ctcaagtgcg actatcgggc aggcgacaat
    13681 gcaccgatgg cctatgtgga gttggtcgat cgtcccgagg agtgaaactc ccggcggagt
    13741 gccgagttga cagtggttcg attcggcacg tcgatcacgg atgatggttg agagaaaacg
    13801 aaaccagtga gatggcctcg ccaccactgg ttttttgatt tatgggtccg tgatccagta
    13861 cgccatgtct gcctgatgcc atcccgcggt gaagaggcga aaatcacctg tttttgctgc
    13921 ctgctggacg gcccttttgc ttttctgcaa aggggggata ttttgtcttg gctgcgccct
    13981 ttgtagtggt gggctgctca tagcggctga tcttgagctg tttgccggcg acccagacct
    14041 tcttgagcag atgcaacagc tctttgggca tgccttgcgg cagtccaact aggctgtagt
    14101 cgtcgaatat ttcgatgcgg ccgatatatt tgctgtcgat gccggcttca ttggcaatgg
    14161 cgccgaccag gttaccgggc ttgaggcctt ggttgcgacc gacttcgacg cggtaattct
    14221 ccagcgaagc atccactgga gcggtcattt ttccgcgttt cggtccggtg tctgatgtcg
    14281 cacgtcgaac gctgggggcc tcgcgttcgc gagtacgacc cccatctcga ctgcgctgtt
    14341 gaagctgctc ttcttcttca cttggttgcg gcagtagcaa ttctccaccg atcagcatgg
    14401 cggcgagtgc cgaggcgatg tcgatcagtg gccgttgact ctgtgtctga tactcctgca
    14461 gcagtttctg atagagcgtc aggtcgaagg tgtcgagggc gctgctgatg tgtgcatgga
    14521 aacgttcgat gcgcttctgg ttgacttcag ccactgtggg caggtacatc cgctcgattt
    14581 tgtgcccagt ggtctgttcg atggcatgca gcaggcggtt ttcgcgctgg gtgacgaaga
    14641 ggatcgcctc accgctgcgt ccagcgcggc cagtgcgtcc tatgcggtgg acgtaggctt
    14701 cgggatcgta ggggatgtcg tagttgatca catgggtcac gcggtcgatg tcgagtccac
    14761 gtgcggcgac atcggtggcg accaggatgg cggtctcgcc acttttgagc cgggcgatgg
    14821 tctgctcccg caggctttgc gggatgtcgc cattgagcgg tgcagcagtg tgtccacggg
    14881 cgttgagctg ctccgccaat gccagggttt cggtcttggt gcgcacgaag atgatgatgg
    14941 catcgtggtc gatggtctcg agaattcggc tcaaggcctc gaacttgtcg acaccgccga
    15001 cgcgccagaa gcgctggcga atcgcttcga ccgtggtggt ctgcgatttg atggcgacct
    15061 cttcggcgtc atgcaggtgc tgccgggcga tacggcggat cggcactggc atggtggcgg
    15121 agaagagcgc aacctgccgc ttgggtggtg tctgttccag gatccattcg acgtcgtcga
    15181 tgaagcccat ccgcagcatt tcatcggctt catcaagcac caggtggttg atggtgccga
    15241 ggtcgagggt gccgcggcgc atgtggtcca tgacccgccc cggggtgccg acgacgacat
    15301 ggaccccctg ccgcaactgt atcaactgct cgcggtagcc ctgaccgccg tagaccgtgc
    15361 agatcctgaa gccaggcaga tggactgcat agctgcggaa ggcttcagac acctgcagtg
    15421 ccagttcgcg ggtcggggcc agaaccagcc cttgcagttg cggacggctg aggtcgatct
    15481 gcgtcagcag aggcagtgca aaggcggcag tcttgccggt gccggtctgt gcttgcccca
    15541 gcaggtcccg acccgccagc agaaagggga tggttcgggc ctggatgggc gaggggcttt
    15601 catagccgag atcatcgagt gcgcgctgga tcggttccgg caggccaagg tcggcaaagt
    15661 tgaggggttg tggagcgtcg gcagtggcag agtgcatcat ggatcgtctg gtaacctgaa
    15721 aatgtaaata ggccgccgcg cggaagatgg gtcatcccgt cgcgggcttg cggagtcggt
    15781 tgggtggctt cccgccagat atgaacgaaa aagtcagtgt ctgttggatg aaggggtgtg
    15841 gctgccgtca gccggcaaga tgttcggcca ggggcagcga gagctcatgc agggcgcggc
    15901 ggtacacctc acgcttgaac tccaccacgc ggctcaacgg atgccagtag ctcacccagc
    15961 gccactggga aaattcggga tggcggctgt gatcgacccg tacccggctc tcttcgccct
    16021 gcatcatcaa caggaaccac ttctgcttct gaccgacgaa gtggctgtca ggctggttgc
    16081 gcaccagtcg cttgggcaac tggtagtgaa gccagccaca ggtgcaggcg aggatgcgga
    16141 catcgtgcgg ctcaagaccg acctcttcat ggagttcgcg gaacagcgcc tgttcaggcg
    16201 attcgccacg ttgaatgccg ccctggggaa attgccatgc ctcttgtcgt ccgatgcgtc
    16261 tggcccaaaa tagttggccc tgtggattgg ccaggatgat gccgacgttg ggacggtagc
    16321 cggatgcgtc gatctcatcg cggctgcggc caccccgcgc cggcgagcgc ggcattataa
    16381 tgccattggc gccggttggg gatggtgcaa gactttcact gttttccata ttttcactat
    16441 tgcgactttc agcggggaat gcaagcagtg ccactggcaa tctttgatct cgacaacacc
    16501 ctgctcggtg gcgacagcga ccatgcctgg ggagagtttc tggtgagcga aggctgggtc
    16561 gatgcggagc gatttcgtgc cagcaacgac ctgttctatc agcgctatct caatggtgac
    16621 cttgatattt ttgagtatca ggcgtttgcc ctggcgccgc tgaaggagat ggggcgcgcg
    16681 atggcagaac agttgcgtac acggtttctg actgagcgca tcgctccgct gattctgccc
    16741 aaggggctgg cgctgctgga gcagcatcgg cgtcgcggcg actttctgtt gatcatcacc
    16801 gccaccagtc acttcatcac cgagccgatt gccagccaac tggcggtgga tgatctgatt
    16861 gccaccatgc cggagctggc cgatggcgag ttcaccggcc gggtggccgg tgttccgagc
    16921 tttcgcgagg gaaaggtggc gcggctgcgg cagtggcttg acgaacgtgc gctgtcgttg
    16981 gatggcagca gcttctacag cgactcgcac aacgatctgc cgctgctgag cctggtggag
    17041 aatccggtgg cggtcgatcc tgacccgctg ctggaggccc acgcgcgcgg cgcgagctgg
    17101 ccggtcatct cgctgcgata gccgctagca ggcgcgcacc gtcaggctct tcaggtactg
    17161 ggtctccggc attgctggat ggatcgggtg atccggtccc tgatgcccct gctcgagaac
    17221 ctgtgccaga cggccgtcat ggcgggccgc actgaccacc acctcccaca gttcggcagg
    17281 ttgcaggtgg ctggagcagg atgaggtgat cagaatgccc tcgtcgctca acagcttgag
    17341 cgcaagctga ttgagctggt ggtagctctt gagcccctga gtgtggaact tgcggcgagg
    17401 gatcaggctc ggcggatcga gcagaataag gtcgaactgg cgccggctgt cgcggcacgc
    17461 cttgaggaag tcgatggcat cgccctgttc ggtgctgacc cgctcggcca cgccattgcg
    17521 gcgggcgttc tcgttgaccc actccagggc atgggccgaa ctgtcgacac aggtgacgct
    17581 gctggcaccg gccagtgccg cctgaatacc ccagccgccg acgtagctgt agaggtcgag
    17641 cacccgccgt gcgcgggcgt ggatgccgag tcgggcgcgg ttgaggcgat ggtcatagaa
    17701 ccagccggtt ttttgcccgc cctgggccgg cgccacgaaa cggccgccat tctcgctgat
    17761 ctcaagctgt tgcggcagtt cgccgctggc cacctcggtg tattcgtcca gcccttcgat
    17821 ggcgcgaaag ccaccttgat tgcgccagat cagcgtggcg ggcgcataga cggccgcgat
    17881 ggcggtttcg agcatcggct tcagctgctc catgccggcg ctgctgatct ggccgaccac
    17941 cacctcgccg tagcagtcga gcaccagtcc cggcaaggca tcgctgtcgc catagaccca
    18001 gcggtggtga tcgctggcga agagttgccg gcgcagccgc aatgcggcct ccagtcgccg
    18061 cgtcagcatc tcttcgtcga tgaccacatc gccctcacgg ctgtagagtc gggccgcaat
    18121 cagggtgttg gggttgacca gggcactgcc gagcggtttg ccctgggcgc tggtgaccgt
    18181 ggccagggtg ccgggggcga actgggtcag cggtgaacgg ttggtgtcga cttcgttgct
    18241 gtagacccag cggtgcccct tcttgatgcg acggtcatgg cctgatttga ggaggagtct
    18301 gggcagggtg ctggtcatgt cgataccgga tgtgaatgaa ggtgaagcaa tcagctcgga
    18361 ttgcggttga gattggcatc ggggcgccag accagatcgg gattgcggct ggcgcgggtg
    18421 tcatccaggc gacgtaccgg caggctgtgt ggtgcctggc gcagccgctc cgggtcgtgt
    18481 gtggcctcgg tgagaatttt tgccatcgca tcgatgaatc catcgagcgt ctccttgctc
    18541 tcggtttcgg ttggctcgat cagcagcgct tcgggcacga tcagcggaaa gtaggtggtg
    18601 ggggcgtggt agccgtggtc gagcagccgc ttggccacat ccattgcagt gatgccaagc
    18661 gccttggcgc tctgttgcag cgtgatgatg aactcatggg tggcgcgccg ctgcggatag
    18721 gccagctcat agccgatctc gaccagacgc tgggcgaggt agttggcgtt gagggtggcg
    18781 tggtcgctga ctcgcgccat gccttcgcgc cccagcatca gcgcataggc gagggcgcgc
    18841 agcaggatgc cggcattgcc catgaagctg ctcagatggc cgatcgaagc gggggcctgt
    18901 gcctgatcga tccagccgaa ccggggttgg ccgtcggcat cgaccacctt gccgacatag
    18961 ggcgtcggca ggtagggcag caaccgctcg ttgaccgcca ccggaccggc accgggtcca
    19021 ccgccgccat ggggggtggc gaaggtcttg tgcaggttga gatgcaggac atcgaagccc
    19081 atcgcacccg gcttggcgcg gccgaggatg gcattgagat tggcgccgtc gtagtagagc
    19141 aggccgccgg cgccatgcac ggcgtcggcg atctcgctga tgccgcgctc gaacaggccg
    19201 caggtcgagg ggttggtcat catcagcccg gcggtgtggg ggccgagcgc cgcccgcagt
    19261 gcggtgaggt cgacatcgcc atcgggcttg agcggaatct cccgcacgcc gtagccgcac
    19321 atcgctgccg tggccgggtt ggtgccatgg gcggcagccg ggatcagaat ctcgctgcgg
    19381 gcatggtcgg cgcgggcctg gtggtaggcg cgaatcatcg ccacgccggc gaactcaccc
    19441 tgggccccgg ccatcggcgt cagtgagaca ccggccatgc cggtcacgct ctgcagaatc
    19501 tcctgcagcc gatggagcgc gccgagaaaa ccctggctga ggctgatcgg cgccagcgga
    19561 tggcggttga gaaagcccgg cagcgaggcg gccttgtggg cgccgcgcgg gttgtacttc
    19621 atcgtgcagg agccgagcgg gtagaagtgg gtgtcgatcg agaagttgcg ctgcgacagc
    19681 cgggtgtagt ggcgcaccac ctgcagttcg gagagttccg gcagcggtgg cggtgtcgca
    19741 cgcagcagtt cggtgggcag gtcgctgcaa tcggccggtc gatcgggcgc ctgtgcatgg
    19801 gcggcgcgct gggggcggct ctggtcaaaa atcagcatgg cggcaactcc gtgagcgggc
    19861 agatcgatca gccgagggcg cgttcgagcg cggcgatgta gcggtcgatg tcggcggcgc
    19921 tgctggtttc ggtggtgcag accagcatgc agtcggacca ctgtgcaaag tcatcggcca
    19981 gtggcacgcc gccgaagatg cgctcatcgg ccaggcgtgc cagcgtctgg cggggatcgc
    20041 gcggcagccg caccaccagc tcatgaaaac aggggccatc aagtggcagc tcgacaccgg
    20101 gaatggcgcg cagccgctcg gccagcgtgc ggcagttgtc gtgacagcgg gcggcgatcc
    20161 gggccagacc cggccccatc aggctcaggt agagggtggc ggctgccacc agcaacccct
    20221 ggttggtgca gatgttggag gtggccttgg cgcggcggat gtgctgttca cgcgcctgca
    20281 gcaccagggt gaaaccaacc cggccgtcgc ggtcgagggt gcgaccgatc agccggccgg
    20341 gcagttggcg caccagcgcc tcacggcagc agacgaaacc gaaggagggg ccgccggagg
    20401 ccatcggaat gcccagtggc tggccatcgc ccaccgcgat gtcggcgccg cggctgcccc
    20461 attgacccgg tggcttcagc agcgccatcg ccagcggatt gaccagcgcg atggcgagca
    20521 ggccgttggc gtgggcccag tcggtcagtt gatcgaccgc ttcgaggcgg cccaggtagt
    20581 tgggctgtgg aatgaccagg gctgcatggt cgtcactcgc agcgggcagt gccttgagat
    20641 cgaccacgcc gctggcgtcg agcggcagca gatcgagttc gatctgctgg ttgcggacga
    20701 tgtcgcgcgc ggcgcgcaga tagagcgggt ggatggtgcc ggcgacccac acccgtcttg
    20761 ccgaggccga gcggttggcg cgcagcgcca tcagaatcgc ctcggccagc gccgacgcac
    20821 catcgtagag cgaggcgttg gagacctcca tcgccatcag cgtggccatc atgctctggt
    20881 attcgtatag cagttgcagc gtgccctggc tcgcctcggc ctgatagggg gtgtaggcgg
    20941 tcatgaattc gccacggccg atgatgtccc agaccgcggc cggaatgtgg tgggcatagc
    21001 agccggcgcc ggcgaagcag agcacattgt catcctgcgc cgccagcgtt cgcagttcgg
    21061 ccagcatctc ccattcactg aggccggcat ccagcccatc gaggctgctg tcgtgcaggt
    21121 gaggcggaat ttcgtcgaac agcgcatcga tgcggtcgac accgatggtg gcgagcatct
    21181 gcgtcacttc ggcttcggta tgggggatga agggcatggg gtggactgct cagagggtgg
    21241 gggagagggc ggggtcagct tcgtgtgcga taagcgtcgc aata
//
LOCUS       DAORCQ010000033        21241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1431_length_21241_cov_5.999009, whole genome shotgun sequence.
ACCESSION   DAORCQ010000033 DAORCQ010000000
VERSION     DAORCQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21241
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_21241_cov_5.999009"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1331
                     /locus_tag="PKI23_05240"
     CDS             147..1331
                     /locus_tag="PKI23_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3322 domain-containing protein"
                     /protein_id="HNN36379.1"
                     /translation="MSWTLPADLRAQVQRLWERGELLRASVRPEAIAWPLRLSLKAPG
                     AADLSDRFEAVRDWVRALAATPQVRIEWREWNHRVQGTQQLPAVVWVDTLHDALAVIG
                     KARAAEPFRALWQRTADEQPALLAWLLKRPLQALELAERWPRLLAVVAWLQGHPRPGV
                     YLRQVDVPGVDSKFIEAHRGVLGELLDLALPPAAIEPAPNGVGHFARRFGFLDKPVRI
                     RFRLLDPALPSLPGCAGLVDITLDAASFAALVLPIERVFITENEVNFLAFPPLARAIV
                     LFGAGYGWDALAGAAWLQRCWLHYWGDIDTHGFAILDQLRGHFPHAASLLMDRETLLA
                     HRAHWGEEPEPAQQDLLRLTAQEATLYDDLRFDRLQPRLRLEQERVGFGWLNAQLPSR
                     LC"
     gene            complement(1371..2501)
                     /gene="flhB"
                     /locus_tag="PKI23_05245"
     CDS             complement(1371..2501)
                     /gene="flhB"
                     /locus_tag="PKI23_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNN36380.1"
                     /translation="MAEEQAQERTEAATPRRLEKAREEGQVARSRDFAMAVLMLVASL
                     GVIASAERLLRLFGELMRFNFRLERSLLFDSSSLVSHLGGSIWQAAVAVGPLLALLFL
                     ASLLGPLAIGGWSVSSKALMPRFNRLDPFAGLKRMFAMRAWVDLLKALAKFLFVTAMT
                     LWLLYRWRSAFHTLGDLPLRPAIGEALHLLGVSFVVLCSSLVLIGLADIPWQLFEYQK
                     QLKMTKQDVREEAKESEGKPEVKGRIRQLQREFARNRMMAEVPTADVIITNPTHFAVA
                     LRYDAERMGAPRLVAKGSDALAMKIREIAQEHGVPIVELPALARAIHHHTEINQEIPA
                     GLYLGVAQVLAYIFQLRRQQQSGGKKPTPPQEVPIPDELRRD"
     gene            complement(2494..3282)
                     /gene="fliR"
                     /locus_tag="PKI23_05250"
     CDS             complement(2494..3282)
                     /gene="fliR"
                     /locus_tag="PKI23_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016263698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNN36381.1"
                     /translation="MLTLSAAAIEKELLGLLLPLFRISAFYLAVPIIGSNLVPRRVRM
                     GLALATTLVVLPTLPAMPALQAFSLAGWWLTIEQVVIGAALGFAVQLLFHAVAMAGQL
                     ISMNMGLGFASMMDPTNGISVPILGQFYLFMVTLLFLASNGHLIVIEATIESFRILPV
                     GAGLPLDGLALLARAGGWIIGAALLISLPAMTTLLVVNLAFGVISRAAPQLNLFSLGF
                     PFTLVFGLLLVWLTLSALSPQFTLLSGEAFELMRALLQNGAAHG"
     gene            complement(3297..3566)
                     /gene="fliQ"
                     /locus_tag="PKI23_05255"
     CDS             complement(3297..3566)
                     /gene="fliQ"
                     /locus_tag="PKI23_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003214552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNN36382.1"
                     /translation="MTPQTVIDIFSSAIQLVVLLVMLIVLPSLLVGLVVSIFQAATQI
                     NEQTLSFLPRLITIFVILMLAGPWILTRLVSYSQTLLGSIPQLIG"
     gene            complement(3576..4328)
                     /gene="fliP"
                     /locus_tag="PKI23_05260"
     CDS             complement(3576..4328)
                     /gene="fliP"
                     /locus_tag="PKI23_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296595.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNN36383.1"
                     /translation="MKRTPLLTLTLLGLLLCATPSWARDLGITALKMTASPDGGQEYS
                     VTLQILIIMTALTLLPALLMMMTAFTRIIIVFSILRQALGLQQTPSNQILLGLALFLT
                     FFVMAPVFKQANEVALTPYLNEQIGFEQALERASEPFRHFMLAQTRRSDLELFVQLSQ
                     QPPVAEPEKIDFATLVPAFVTSELKTAFQIGFLLFIPFLIIDLVVASVLMAMGMMMLS
                     PVIISLPFKIMLFVLIDGWVLLIGNLAASFAT"
     gene            complement(4325..4765)
                     /gene="fliO"
                     /locus_tag="PKI23_05265"
     CDS             complement(4325..4765)
                     /gene="fliO"
                     /locus_tag="PKI23_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNN36384.1"
                     /translation="MKRLSRTAIASLTLLLSGVSMALRAAEHSAVAEPLSSSQSIMQM
                     LSGLAVVLALFIALAWLLKRFGRGQLHGGGTLFRTLGAMPVGTRERLLLIEIGGEQFV
                     LAVTPGQISKIHHFEQPIALPEPGSDSDFATRLKSLIEQRRSAP"
     gene            complement(4778..5248)
                     /gene="fliN"
                     /locus_tag="PKI23_05270"
     CDS             complement(4778..5248)
                     /gene="fliN"
                     /locus_tag="PKI23_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016781565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNN36385.1"
                     /translation="MSNEKGSGRSDEELAAQWGEALSEQDGDSDWGAALDEQKKPDNI
                     YGRAKPVKLEELSSEGGHSTGPADLSNPDLDVILDIPVTISMEVGSTPITIRNLLQLS
                     QGSVIELDRLAGEPLDVLVNGTLIAHGEVVVVNEKFGIRMTDVVSPSERINRLR"
     gene            complement(5282..6250)
                     /gene="fliM"
                     /locus_tag="PKI23_05275"
     CDS             complement(5282..6250)
                     /gene="fliM"
                     /locus_tag="PKI23_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNN36386.1"
                     /translation="MSLQDLLSQDEIDALLHGADDGAKGDDRLGLPGEITPYDLATQD
                     RIVRGRMPTLEMINERFARYIRISLFNLLRRSAEVQVGGVQVMKFGEYIHTLYVPTSL
                     NMVKFRPLRGTALFILDAKLVFKLVDNYFGGEGRHAKIEGREFTPTENRIVQLMLGYV
                     FKDLCEAWSPVLKIDLEYVNSEVNPAMANIVTPSEAVVVSTFHIELDGGGGNLHITLP
                     YSMVEPIRDILDAGMQSDSNEVDDRWAKILRNDILNSCVDMNCTLYQIPASLRDVKNL
                     KPDQVLGVEMPEEVTLHANGVPIFVCKLGVSNGKYALKVLRKAELI"
     gene            complement(6267..6878)
                     /locus_tag="PKI23_05280"
     CDS             complement(6267..6878)
                     /locus_tag="PKI23_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNN36387.1"
                     /translation="MAKEKENDQEQEAKAGGGNKKAILIIALVAVVCLLLGGGGGYLL
                     FSGGEKESKSTAGGKKSDKASKSEKSAKKEEGEEEAATDAEEGEEGTEEASPKPPAQY
                     SNMKPLTVNYTVNGKPRYLQVSISVMSRDPAVNSFVEANQPLLQNNLLLIMGNYSFDQ
                     LGTLEGKEKLRAACFDEVQKVIHKELGEEHNIEQVLFFNFILQ"
     gene            complement(7113..7970)
                     /locus_tag="PKI23_05285"
     CDS             complement(7113..7970)
                     /locus_tag="PKI23_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN36388.1"
                     /translation="MTQEPAWYRDAIATPFVEGVVEVAGCPIHYQHWSNPGAPALLLV
                     HGYAAHSHWWDFIAPLLLDQYQVVALDISGCGESGHRDHYDLATYAAELMAVAEATRL
                     PEPFTLIGHSFGGGIVLKAATLYPERLRGIVLVDSSVVRPPGEASQRRTIGALPTIVH
                     PDLPTAIERFRLIPPQHCPNQFLVDYIARHSYRQSSEGWQLKSDAKLLGRFEREDQTD
                     ALLALRCRFGVIHGGLSSTFRAEQRDYLAYVAPPGSPFVELPGANHHLFIDQPLEFAA
                     QLKTLLAQW"
     gene            complement(8132..8776)
                     /gene="coq7"
                     /locus_tag="PKI23_05290"
     CDS             complement(8132..8776)
                     /gene="coq7"
                     /locus_tag="PKI23_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017476331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNN36389.1"
                     /translation="MNSYRRLSRFDRLLGELQHCVETLSRTSTSARPTPGAQLPEADL
                     SERERRHAAGLMRVNHAGEVCAQALYQGQALTARLHSVRSQMEQAAEEERDHLAWCEA
                     RLEALQSHTSRLNPLWYGLSFGLGAAAGAGSDRVSLGFVAATEELVGRHLEAHLDTLP
                     EADAKSRAVVEEMLCDELQHARSALDAGGFDFPAPIKLAMRLASRVMTHSSYHL"
     gene            complement(8861..10219)
                     /locus_tag="PKI23_05295"
     CDS             complement(8861..10219)
                     /locus_tag="PKI23_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HNN36390.1"
                     /translation="MTQDYRCFKAYDIRGRIPDQLNDEIAWRIGRAFAAYLQPRKVVV
                     GHDIRLSSPQMAAEVMRGLRESGVDVYDIGQCGTEEVYFATFDYQFDGGIMVTASHNP
                     KDYNGMKLVRDNARPISGDTGLFEIKRLAERGEFVDAEVQGSHYTLQHRARYITHLLG
                     YIDASALKPLKLVVNAGNGGAGEVIDALEPLLPFEFIKLHHAPDGHFPNGVPNPLLPE
                     NRPATIEAIRTSGADLAIAWDGDFDRCFLFDETGAFIEGYYIVGLLAEAFLRKAPGSR
                     IVHDPRLTWNTIDIVERLGGSPIQSKTGHAFIKERMRAEDAIYGGEMSAHHYFRDFAY
                     CDSGMIPWLLTAELISRSGKPLSQLVRERMAKFPVSGEINRRVEQPERLIAEIEQRFA
                     AQAVDLDHTDGLSMQFDDWRFNLRMSNTEPVLRLNVESRGDPALMERKTAELLALIGG
                     VA"
     gene            complement(10246..11073)
                     /gene="galU"
                     /locus_tag="PKI23_05300"
     CDS             complement(10246..11073)
                     /gene="galU"
                     /locus_tag="PKI23_05300"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNN36391.1"
                     /translation="MIKKCLFPVAGYGTRFLPATKSMPKEMLPVVNKPLVQYAVEEAV
                     EAGLTEIAFVTGRSKRSIEDHFDKSYELEHQIAGSGKERYLTGIRKIMDSCTFSYTRQ
                     REMKGLGHAILTGRTLIGEEPFAVVLSDDLCINQEEAVLTQMVKLYKQFRCSIVAIEE
                     VPPEETNKYGVIAGEEMKEGLYRVHDMVEKPKPEDAPSNLAIIGRYILTPDIFDILEK
                     TPPGKNGEIQITDALLTQAKDGCVLAYKFRGKRFDCGSVDGFIEATNYCYQHCYRDI"
     gene            complement(11136..12125)
                     /gene="galE"
                     /locus_tag="PKI23_05305"
     CDS             complement(11136..12125)
                     /gene="galE"
                     /locus_tag="PKI23_05305"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008041069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNN36392.1"
                     /translation="MRLLVTGGAGYIGSHVTRLLGEAGHEVVVLDNLSTGFRQAVLSG
                     ELVVGDLCDTALLDRLFRQHRFEGVLHFAANIVVPESVANPLKYYRNNSFNVLNLLEV
                     MHKHEPRYLIFSSTAAVYGMPQSALVSEESPLQPINPYGASKMVSERMIIDHAAVSGL
                     RHVILRYFNVAGADPGGRIGQSTPAATHLIKVAAQTALGLRPALQIFGTDYPTPDGTC
                     IRDYIHVEDLASAHLAALNHLAAGGTSELLNCGYGHGLSVREVVQSVKQVTGVDFAVQ
                     TSDRRPGDPAQLAADNRRILATLDWRPRHDDLETIVRSAVDWERRQLNHPWRN"
     gene            complement(12218..14254)
                     /gene="ligA"
                     /locus_tag="PKI23_05310"
     CDS             complement(12218..14254)
                     /gene="ligA"
                     /locus_tag="PKI23_05310"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNN36393.1"
                     /translation="MSEPPAAAEPAQQIRRLRQAIEQHNYNYYVLDQPTVPDAEYDRL
                     LRELQALEAAHPELVTPDSPTQRVGAAPLASFAEVQHALPMLSLENLFTADEARQFDQ
                     RLRERLGLDVAIDYSCEPKLDGVALSLRYQRGQLLQAATRGDGFRGEDVTLNVRTIRA
                     IPLRLAGNDHPEQLEVRGEVYLPLAGFEQLNQRARAAGEREFVNPRNAAAGSLRQLDP
                     RITATRPLRFCSYGVGLVEGGPLPDRHSAIMQQLQQWGLPTSREAALVQGAEGCLDYY
                     RRLQQRRGELGFEIDGVVYKVDRLDWQQQLGFVSRAPRWAAAHKFPAQEELTELLDVD
                     FQVGRTGAVTPVARLKPVFVGGVTVSNATLHNMEEIARLDLHIGDTVIVRRAGDVIPK
                     VVQCVPERRPATARPIQLPSQCTQCGSAILKPPGEVVARCSGGLYCPAQRKEAIRHFA
                     SRLALDIQGLGEKLIDQLVEQRLVESVADLYRLTTAQLTPLERMGEKSASRIVAAIDA
                     SRQTTLARFLYALGIREVGEATANALANHFGELDAVMAADRETLQQVADVGPVVAEQI
                     HTFFRQPHNLEVIAVLRSAGIHWPAISPATAGTGPLTGKTVVITGTLATLSRDAAKAQ
                     LTAQGAKLTDSLSARTDYLVVGENPGSKLARAQQLGTPQLDEAAFIELLSQSRC"
     gene            complement(14251..15033)
                     /locus_tag="PKI23_05315"
     CDS             complement(14251..15033)
                     /locus_tag="PKI23_05315"
                     /inference="COORDINATES: protein motif:HMM:NF016257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNN36394.1"
                     /translation="MRDWLFMIGGLLILIILADGFRRVWRARRNAIRVRLEEDVSFDP
                     LEELDLLRGELPSGGARPKVRACNGHEPEPAPATESATDSTPPQRPVSPMTQQLLFED
                     ECDTTADEVEEAPIEPEVLAIHLLAPAPVNGQRLLELLQQQRLEFGERAIFHCHKQRG
                     GNKGELLFSVANLLEPGTFDPEQADQLETIGVVLFLTLPGPARPLDAFDEMRRMAELL
                     RRALDGELRTSTQEPFNTQAVRSYRATIRRFIEKTTSASAAQ"
     gene            complement(15130..18648)
                     /gene="smc"
                     /locus_tag="PKI23_05320"
     CDS             complement(15130..18648)
                     /gene="smc"
                     /locus_tag="PKI23_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNN36395.1"
                     /translation="MRLQSIKLAGFKSFVDPTTVAFATNLSAVVGPNGCGKSNIIDAV
                     RWVMGESSARQLRGEQMADVIFNGSTHRKPVGQASIELLFDNSAGSLGGEYARFAEIS
                     IRRKVTRAGQSSYYLNGVRCRRRDIVDIFLGTGLGPRSYAIIEQGMISHLIEARPDDL
                     RVYIEEAAGISKYKERRRETENRMQHTKENLQRINDIKEELQRQLQTLERQARAAQKY
                     RELKEEERSQRIRLAALRWRALEQQRLRLDDEVSKESLLRDGLLAEQSRLERELEQLR
                     LRHLELHEAFNLEQGHYYRQGAEISRLEQGIRHAQERARQLHTTLADNERQMQALHAQ
                     QAHSQQLGTTLSDTLQDLTPRSEAAQVAEQQAAQQLAATEQRMQQWQEGFDRLQREAQ
                     LPARQTEAARATLRQIEQQLPRLKSRIERLHGERDSLRGQIDDTALPALRQRFDQLAD
                     EIRLSEVARQASEQRRQQLRQQRNALQPRLDQLRRLDQQLSGRISSLETLQQAALGRG
                     RLDPRQTQLLQRLELDRLPRLGERLEVEAGWEGAIERLLAHRIRALCLPAERADEPER
                     LMAALFELPLALLTTAHAPAQPPPARWPRLSERLRSSHEIADWLHGGYCADNLASALD
                     RRGQLGPGEFFLTPDALLIGRHAIERLPDDREGGGALLRARELTELKREQQQLKEQLQ
                     DAQQQLEEFDRHLRQCEAELAQTQSRTTEQRRQQATLQADLSARQARIEQSQHRITAI
                     DEELTSATREIDEDQARQARTQAALNQAIAQSGEFEQQRQRLRAEQEQLREALAQARS
                     HERDCRDQQHRLALERQRCLAELKAAQAQSARLEQEIERATAQRQQLQMAIADLVEPQ
                     QALQQQLDQALAAQLMAEQRLAAARQAVEQSERQQREQEDQRRHLDSQLRSLTQQVDE
                     KRLQLRELALHDNALAEQLRSLGSSVDLIAGSEEPLDAMQLEQALERLLQRIDRLGAI
                     NLAAIDEHRIQSERKGYLEAQQADLGEALETLQEAIRKIDRETRSRFRDTFDQINRGL
                     QALFPKVFGGGHAELVLTSDELLDSGVAIMAQPPGKRNSTIHLLSGGEKALTAIALVF
                     AIFQLNPAPFCLLDEVDAPLDDANVGRYVNLVREMSHGIQFIYITHNKIAMEMAHQLL
                     GVTMQEPGVSRLVTVDIEAAQALAGA"
     gene            19055..20188
                     /locus_tag="PKI23_05325"
     CDS             19055..20188
                     /locus_tag="PKI23_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNN36396.1"
                     /translation="MGPLAGVKIIEIAGIGPGPFCGMMLADMGAEVIRVDRASAPANA
                     PRSNDVLARGRRSIALNLKKPEAIETVLKLCEQADAVFEGFRPGVAERLGIGPDACMG
                     RNPKLVYGRMTGWGQDGPMSQAAGHDINYISLSGALHAIGPKGGKPVPPLNLVGDFGG
                     GGMLLAFGMVCALLEAGRSGRGQVVDASMVEGSAALMAMIYSFASGGGHSEERGTNML
                     DGGTHFYDTYETRDGKYISLGSIEPQFYALLIEKAGLDPQKFAPQMDRNKWPELKEEL
                     TRVFKTRSRDEWCAIMEGSDVCFAPVLSFREAPSHPHNVARNSFVEINGVMQPAPCPK
                     FSRSAPEQPRPPRRAGEDTEAVLSDFGFSSGQIASLRAAGALA"
     gene            complement(20284..21210)
                     /locus_tag="PKI23_05330"
     CDS             complement(20284..21210)
                     /locus_tag="PKI23_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36397.1"
                     /translation="MHTATAPTRFTQLHIDIARFSTDDFNPFHDQKLWWQIPGTPFSA
                     PLVLGFQLAAWIEDRLRQHRQQADEARLIEQHGLRYSNYQFDFVSPVLPGDEVTLEIK
                     PSTLVTSPTTLLRNRLLLRAGQRTALLGHKRESRQPLVLPEGTPPLPDPLSAVTDRDH
                     LPGQACFVKRKFTMTGNGKNFLLGAQVDPTHYFNELEDRVVFPEMFPLAYLSCALLER
                     GLKAGEDFMQRPRLYASQQFSVDRQLMRRVKSNSVLYMLVCEPLPAASSTGLRGRTAA
                     HCCFPCFGVLEDGALLYRALMTLVPLAAIGSG"
BASE COUNT         3405 a         6647 c         7176 g         4013 t
ORIGIN      
        1 aagatccaca tcatcgagcc cttcgtcgcc agcgtcggct tcgtgcacaa cgaggacggg
       61 cgcgactcgc gcttgcgctg cctgaccatc gccgagtacc gggcgcagaa actggcccac
      121 gcggccgacc tggcgggcgc gacggcgtga gctggacgct gccggccgac ctgcgcgccc
      181 aggtgcagcg cctgtgggag cgcggcgaac tgctgcgcgc cagcgtgcgt ccggaggcca
      241 tcgcctggcc gctgcgcctg agcctgaagg cgccgggcgc ggcggatctc tccgaccgct
      301 tcgaggcggt gcgcgactgg gtgcgcgcac tcgccgccac gccgcaggtg cggatcgaat
      361 ggcgcgagtg gaaccatcgg gttcagggca cacagcagtt gccggccgtg gtctgggtcg
      421 acaccctgca cgatgcgctg gccgtcatcg gcaaggcccg ggcggccgag ccgttccggg
      481 ccctgtggca acggacggcc gatgaacagc cggcgctgct ggcgtggctc ctgaagcgac
      541 ctttgcaggc tttggaattg gccgagcgct ggccgcggct gctggccgtc gtcgcctggc
      601 tgcaggggca tcctcgcccc ggcgtctacc tgcgccaggt ggatgtaccc ggcgtggaca
      661 gcaagttcat cgaggcgcat cgcggcgtgc tcggcgagct gctcgacctc gccctgccgc
      721 ccgcggccat cgaacccgca ccgaacggcg tgggtcactt cgctcgccgc ttcggcttcc
      781 tggacaagcc ggtgcggatt cggttccgtc tgctcgaccc ggcgctgccc agcctgccgg
      841 gctgcgcggg attggtcgac atcacgctgg acgcggcgag cttcgcggcg ctcgtgttgc
      901 cgatcgagcg cgtgttcatc accgagaacg aggtgaactt tctcgccttt ccgccgctgg
      961 cgcgcgcgat cgtcctgttc ggcgccggct acggctggga cgccctggcc ggcgccgcct
     1021 ggctacagcg ctgctggctc cactactggg gtgatatcga cacccacggc ttcgccatcc
     1081 tcgaccagtt gcgcggccac tttccccacg cggcgtcgtt gctgatggat cgggagactc
     1141 tgctcgcgca tcgggcgcat tggggcgagg agcccgagcc cgcacagcag gacttgctcc
     1201 gtctgacggc gcaggaggcg acgctctacg acgacctcag gttcgatcgc ctgcaacccc
     1261 ggctgcggct ggagcaggag cgcgtgggtt ttggctggct gaacgcgcag ttgcccagca
     1321 ggttgtgctg agactgaatt tggttctgtt gacgatccgt cgggcgtggc tcaatcgcgg
     1381 cgcagttcgt cggggatcgg gacttcctgc ggcggtgtcg gcttcttgcc gcccgactgc
     1441 tgctggcggc gcaactggaa gatgtaggcc agcacctggg ccacgccgag gtagagtccg
     1501 gccggaatct cctggttgat ctcggtgtgg tggtggatgg cccgtgccag tgccggcagc
     1561 tcgacgatcg gcacgccgtg ttcctgggcg atttcgcgga tcttcatcgc cagcgcatcg
     1621 ctgcccttgg cgaccagtcg cggtgcgccc atccgctcgg cgtcgtagcg cagcgcgacg
     1681 gcaaagtggg tcgggttggt gatgatgacg tcggcggtgg gcacctcggc catcatccgg
     1741 ttgcgggcga attcgcgctg caactggcgg atgcgcccct tcacctcggg tttgccttcg
     1801 ctctccttgg cctcttcgcg cacatcctgc ttggtcatct tcaactgctt ctgatactcg
     1861 aacagctgcc aggggatgtc ggccagtccg atcagcacca ggctgctgca cagcacgacg
     1921 aagctgacac cgagcagatg gagcgcctcg ccgatggccg ggcgcagcgg caggtcgccc
     1981 agggtgtgaa aggcgctgcg ccagcggtag agcagccaga gggtcatggc ggtgacgaag
     2041 agaaacttgg ccagcgcctt gagcaggtcg acccaggcgc gcatggcgaa catccgcttg
     2101 aggccggcga aggggtcgag ccggttgaag cgcggcatca gcgccttgct gctgaccgac
     2161 cagccgccaa tcgccagcgg tcccagcagt gaggcgagaa agagcagcgc cagcaggggt
     2221 cccaccgcca cagcggcctg ccagatcgag ccgccgaggt ggctgaccag gctgctgctg
     2281 tcgaacagca ggctgcgctc cagacggaag ttgaaccgca tcagctcgcc aaagagccgc
     2341 agcagccgtt cggcgctggc gatcaccccc aggctggcga ccagcatcag cacggccatg
     2401 gcaaagtcgc gcgaacgggc gacctgcccc tcttcgcggg ctttttccag ccgtcggggg
     2461 gtggccgctt cggtccgttc ctgggcttgt tcttcagcca tgcgccgccc cgttctgcag
     2521 gagggcacgc atcagctcga aggcttcgcc gctcagcagc gtgaactggg gtgacagggc
     2581 cgacagcgtg agccagacca gcagcaggcc gaagaccagc gtaaagggaa agccgagcga
     2641 gaagaggttg agctgcggcg cagcgcggct gatgacgccg aaggcgaggt tgaccaccag
     2701 cagcgtggtc atcgccggca gtgagatcag cagtgcggcg ccgatgatcc agccgccggc
     2761 gcgggccagc agcgccaggc cgtcgagcgg cagtccggcg ccgaccggca ggatgcggaa
     2821 gctctcgatg gtcgcttcga tgacgatcag gtggccgttg ctggcgagaa acagcagggt
     2881 caccatgaac aggtagaact ggccgaggat cggcaccgag atgccgttgg tcgggtccat
     2941 catcgaggcg aatcccagcc ccatgttcat gctgatcaac tggccggcca tggccacggc
     3001 gtgaaacagc agctgcacgg caaagccgag tgcggcaccg atcaccacct gttcgatggt
     3061 cagccaccag ccggcgagcg agaaggcctg gagggcgggc atggcgggca gtgtcggcag
     3121 caccaccagg gtggtggcca gtgccagccc catgcgcacg cgacgcggga ccagattgga
     3181 gccgatgatc ggcacggcaa ggtagaaggc ggagatgcgg aacagcggca gcagcagccc
     3241 cagcagctct ttttcgattg cggccgcaga gagggtcagc atgggcggcg tgccggctag
     3301 ccgatcagtt gcggaatgct gccgagcagc gtctgggaat aggagaccag ccgtgtgaga
     3361 atccagggtc cggccagcat caggatgacg aagatggtga tcagccgtgg cagaaagctc
     3421 agcgtctgtt cgttgatctg ggtggcggcc tgaaagatgc tgacgacgag gccgaccagc
     3481 aggctgggca gcacgatcag catcaccagc agcacgacga gctgaatggc gctggagaag
     3541 atgtcgatga ccgtctgtgg cgtcatgggc gtgccctagg tggcgaagct ggcggcgagg
     3601 ttgccgatca gcagcaccca gccgtcgatc agcacgaaca gcatgatctt gaacggcagc
     3661 gagatgatga ccggcgacag catcatcatc cccatcgcca tcagcacgct ggcgacgacg
     3721 aggtcgatga tcagaaaggg aatgaacagc agaaacccga tctggaaggc ggtcttcagc
     3781 tcgctggtga cgaaggctgg caccagggtg gcgaagtcga ttttctctgg ctcggcgacg
     3841 ggcggttgct ggctcagttg cacgaagagt tcgaggtcgc tgcggcgggt ctgcgccagc
     3901 atgaagtggc gaaagggctc cgaggcgcgt tcgagcgcct gctcgaagcc gatctgctca
     3961 ttcagatagg gtgtcagtgc cacttcgttg gcctgcttga agaccggcgc catgacaaag
     4021 aaggtcagaa acagcgccag cccgagcaga atctggttgg agggggtctg ttgcagtccc
     4081 agtgcctgac gcagaatcga aaagacgatg atgatgcggg tgaaggcggt catcatcatc
     4141 aacaacgccg gcagcagcgt cagcgcggtc atgatgatca gaatctgcag ggtcaccgag
     4201 tactcctgac caccatcggg gctggcggtc atcttcagtg cggtgatgcc aaggtcgcgg
     4261 gcccagctcg gcgttgcgca gagcagcagg cccagcaggg tcagcgtcag cagcggggtg
     4321 cgtttcatgg tgcgctccgg cgctgctcga tgaggctttt cagtcgggtg gcgaaatcgc
     4381 tgtcgctgcc cggctccggc agggcgatcg gctgttcgaa gtggtggatc ttgctgatct
     4441 ggccgggtgt gaccgccagc acgaactgct cgccgccgat ctcgatcagc agcagccgct
     4501 cacgggtgcc gaccggcatg gcgcccagcg tgcggaacag cgtgccgccg ccgtggagct
     4561 ggccgcggcc aaagcgcttc agcagccagg ccaaggcgat gaagagtgcc agcaccaccg
     4621 ccaatccgga cagcatctgc atgatcgatt gcgacgacga cagcggttcg gccacggcgc
     4681 tgtgctcggc agcccgcagc gccatgctga caccgctcag cagcagggtc agagaagcga
     4741 tggcggttct ggaaagtctc ttcatggcgg ctccggttca acgcagccgg ttgatgcgtt
     4801 cggaagggct gaccacgtcg gtcatgcgga tgccgaactt ttcgttgacc acgaccactt
     4861 cgccatgggc gatcagggtg ccgttgacca gcacatccag gggttcaccg gccagccggt
     4921 cgagttcgat caccgagccc tgacttagtt gcagcaggtt gcggatggtg atcggcgtgc
     4981 tgccgacctc catgctgatg gtgaccggga tgtcgagaat cacatcgaga tcggggtttg
     5041 agaggtctgc cggacccgtg ctgtgccccc cctcactgct cagctcttcg agtttgaccg
     5101 gtttggcccg gccgtagatg ttgtccggtt ttttctgctc atccagcgcc gctccccagt
     5161 cgctgtcgcc atcctgttca gagagcgcct cgccccactg ggccgccagc tcttcgtcgc
     5221 tgcgtccgga acctttttca ttgctcatgg cagtgtctcc atgttcgatt catcgtgatg
     5281 actagatcag ttcggccttg cgcagcacct tcagcgcata cttgccattg gagacgccga
     5341 gcttgcagac gaagatcggc acgccattgg cgtggagggt cacctcttcg ggcatctcga
     5401 cgcctagcac ctgatcgggt ttgaggtttt tcacatcccg cagcgatgcc ggtatctggt
     5461 agagcgtgca gttcatgtcg acacaggagt tgagaatgtc gttgcgcagg attttggccc
     5521 agcgatcgtc gacctcgttg ctgtcgctct gcatgccggc atcgagaatg tcgcggatcg
     5581 gctcgaccat tgaatagggc agggtgatgt gcaggttgcc gccgccgcca tcgagttcga
     5641 tgtgaaaggt gctgaccacc actgcttcgc tcggcgtgac gatgttggcc atcgccgggt
     5701 tcacctcgga gttgacatac tccagatcga tcttgaggac cggcgaccag gcctcgcaca
     5761 gatccttgaa gacatagccc agcatcagtt gcacgatgcg gttttcggtc ggggtgaatt
     5821 cacggccctc gatcttggcg tgacgcccct cgccgccgaa gtagttgtcg accagcttga
     5881 agaccagctt ggcgtcgaga atgaacagcg ccgtgccacg cagcggcctg aacttcacca
     5941 tgttcaggct ggtggggaca tagagggtgt gaatgtactc accgaacttc atcacctgca
     6001 cgccgccgac ctgcacctcc gcgctgcgtc gcagcaggtt gaacaggctg atgcggatgt
     6061 agcgggcgaa gcgctcgttg atcatctcca gtgtcggcat ccggccgcgg acgatgcggt
     6121 cctgggtggc cagatcatag ggggtgattt cgcccggcag accgaggcgg tcatcgccct
     6181 tggcgccatc atccgcgcca tggagcagcg cgtcgatttc gtcctggctg agaaggtcct
     6241 gtaggctcat cggcgatctg cttggcttac tgaagaatga aattgaagaa cagcacctgt
     6301 tcgatgttgt gctcttcacc cagctctttg tggatcacct tctggacctc atcgaagcag
     6361 gcggcacgga gcttctcctt gccttccagc gtgcccagtt ggtcaaagct gtaattgccc
     6421 atgatcagca gcaggttgtt ctgcagcagc ggttgattgg cctcgacgaa gctgttgacg
     6481 gccgggtcgc ggctcatcac cgagatggag acctgcaggt agcgtggctt gccattgacg
     6541 gtatagttga cggtcagcgg cttcatgttg ctgtactgcg ccggcggctt ggggctggcc
     6601 tcttcggtgc cctcttcgcc ctcctcggca tccgtggcgg cctcttcttc gccctcctct
     6661 ttttttgccg atttttcgct tttgctcgcc ttgtcggatt ttttgccgcc ggccgtgctc
     6721 ttgctctctt tctccccgcc cgagaacagc aggtagccgc caccgccgcc gagcagcaga
     6781 cagaccactg cgaccagcgc gatgatcagg atggccttct tgttgccgcc gccagctttc
     6841 gcttcctgtt cctgatcgtt ttctttctct tttgccatgg gttgcctgcg tcaaaaatcg
     6901 gatgacacca ggtcatagca agcacgatgc caagcgaaga tgagggcgaa ttggccggga
     6961 atggcgtgtg aaactgtggc tctggccgtc acttccttgg cgaggcatgg gcaggtcagg
     7021 cgaatggacg gttttctgac gcagtgccgt tcaatcgaga cggaattccg ctgccggatg
     7081 gcggttgcgg acgctccgtg gtcttcggtc tgctaccact gtgccagcag cgttttgagc
     7141 tgcgccgcaa actccagcgg ctggtcgatg aagaggtgat ggttggcgcc gggcagttcg
     7201 acgaagggcg aacccggcgg ggccacatag gccagatagt cgcgctgttc cgcgcgaaag
     7261 gtcgagctca ggccaccatg gatcacgccg aagcgacagc gcagcgccag cagcgcatcg
     7321 gtctgatcct cccgttcgaa acggccgagc agtttggcat cgctcttcag ttgccagcct
     7381 tcgctgctct gccggtagga gtggcgggcg atgtaatcga ccagaaactg gttcgggcag
     7441 tgctgcggcg ggatcaggcg gaaccgctcg atggcggtgg gcaggtcggg atggacgatg
     7501 gtcggcagcg caccgatggt acggcgctgc gatgcttcgc ccggcgggcg gaccaccgag
     7561 gagtcgacca gcacgatgcc gcgcaaccgt tccggataga gtgtcgccgc cttgaggacg
     7621 atgccgccgc cgaagctgtg gccgatcagc gtgaagggct cgggcagccg ggtcgcctcg
     7681 gcaacggcca tcagctcggc ggcataggtg gccaggtcat agtggtcgcg gtgaccgctc
     7741 tcgccgcagc cggagatgtc gagcgccacc acctggtact ggtcgagcag cagcggcgcg
     7801 atgaagtccc accagtgcga atgggcggca tagccatgca ccagcagcag cgccggcgcg
     7861 ccggggtttg accagtgctg gtagtgaatc gggcagccgg ccacctcgac cacgccttcg
     7921 acaaaggggg tggcgattgc atcgcggtac caggcgggtt cctgagtcat ggctcggctc
     7981 ttttggggtg ggtgggcaca aggcgtcgct ccggcagcgg cctgagagtg tacggcctcg
     8041 atgcgcccgg ataaaagggc tctcgcggaa attcaccgtg accgcccggc actggccgaa
     8101 gcgcggcggt gccgggctaa acagcagccg gttacaggtg gtagctgctg tgggtcatca
     8161 ccctggaggc cagacgcatg gccagtttga tcggcgcggg aaagtcgaat ccgccggcat
     8221 cgagtgcact gcgggcatgc tgtagctcat cgcacagcat ctcctcgacc accgcccggc
     8281 ttttggcatc cgcctccggc agggtgtcga ggtgggcttc gaggtgtctg cccaccagct
     8341 cttcggtggc ggcgacgaaa ccgaggctga cccggtcgct ccccgcaccg gcagcggcgc
     8401 ccaggccgaa cgacaggcca taccagagtg gattgagccg gctggtgtga ctctgcagtg
     8461 cctccagccg cgcttcgcac caggccagat ggtcgcgctc ctcctccgcg gcctgctcca
     8521 tctggctgcg cacgctgtgc agacgtgcag tcagcgcctg cccctggtag agcgcctggg
     8581 cacagacttc accggcatgg ttgacccgca tcaggccggc ggcgtgccgc cgttcgcgct
     8641 cgctcagatc ggcttctggc agctgcgcac ccggtgtggg gcgggccgag gtcgatgtac
     8701 gcgacagcgt ctcgacacag tgttgcagtt cgcccagcag gcgatcgaac cggctcagcc
     8761 gacggtagga gttcattggt acgctccgat ggtgatgggc tttgcaggtg gcatgatagc
     8821 ctcccggctg ccgattgggg caggcgtgtg gcccgcccgg ctacgccacg ccgccgatca
     8881 gcgccagcag ctcggcggtc ttgcgctcca tcaatgccgg atcaccgcgc gactcgacgt
     8941 tgagccgcag caccggttcg gtgttggaca tgcgcaggtt gaagcgccag tcgtcgaact
     9001 gcatgctcag gccatcggtg tggtcgagat cgactgcctg cgcagcgaag cgctgttcga
     9061 tctcggcaat cagccgttcc ggctgttcga cccggcggtt gatctcgcca ctgaccggga
     9121 atttcgccat ccgctcgcgc accagttgcg acagcggctt gccgctgcgg ctgatcagct
     9181 cggcagtcag cagccagggg atcatgccgc tgtcacagta ggcaaagtcg cgaaaatagt
     9241 ggtgggcgct catctcgccg ccatagatgg catcttcggc ccgcatccgc tccttgatga
     9301 aggcgtggcc ggtcttgctc tggatcgggc tgccgcccag ccgttcgacg atgtcgatgg
     9361 tgttccaggt caggcgtgga tcgtggacga tccggctgcc gggggccttg cgcagaaagg
     9421 cttcggccag caggccgacg atgtagtacc cctcgatgaa ggcaccggtt tcgtcgaaca
     9481 gaaagcagcg gtcgaagtcg ccatcccatg cgatcgcgag gtctgcgccg ctggtgcgga
     9541 tcgcctcgat ggtggccggc cggttctccg gcagcagcgg gttgggcacg ccgttgggaa
     9601 agtggccatc gggcgcatgg tgcagcttga tgaactcgaa cggcaacagc ggttccagtg
     9661 cgtcgatcac ttcaccggcg ccaccgttgc cggcgttgac caccagcttc agcggcttga
     9721 gtgcgctggc atcgatgtag ccgagcaggt gggtgatgta acgtgcgcgg tgttgcagcg
     9781 tgtagtggct gccctgaacc tcggcatcga caaactcgcc acgctcggcc agccgtttga
     9841 tctcgaacag gccggtgtcg ccgctgattg gccgggcgtt gtcgcgcacc agcttcatgc
     9901 cgttgtaatc cttgggattg tggctggccg tgaccatgat gccgccatcg aactggtagt
     9961 cgaaggtggc aaagtagacc tcctcggtgc cgcactggcc gatgtcatag acatcgaccc
    10021 cgctctcgcg caggccgcgc atcacctcgg cggccatctg cggactggag aggcggatgt
    10081 cgtgcccgac caccaccttg cgtggctgga ggtaggccgc gaaggcgcgg ccgatgcgcc
    10141 aggcgatctc gtcattgagc tggtcgggga tgcggccgcg gatgtcgtag gccttgaagc
    10201 agcggtagtc ctgggtcatg ccatcttcct tcatggatgc agcgatcaga tgtcgcggta
    10261 gcagtgctgg tagcagtagt tggtggcttc gatgaaacca tcgacgctgc cgcagtcgaa
    10321 ccgtttgcca cggaatttgt aggccagcac gcaaccatcc ttggcctggg tcagcagggc
    10381 gtcggtgatc tggatctcgc cattcttgcc cggtggggtc ttctcgagaa tgtcgaagat
    10441 gtcgggcgtc aggatgtagc ggccgatgat ggcgaggttg ctgggggcat cctcgggctt
    10501 gggtttttcg accatgtcgt ggacgcggta gagcccctcc ttcatctctt cgccggcgat
    10561 gacgccatat ttgttggtct cctcgggcgg cacctcctcg atggcgacga tgctgcagcg
    10621 aaactgcttg tagagcttca ccatctgcgt cagcaccgcc tcctcctggt tgatgcagag
    10681 gtcgtccgag agcaccaccg cgaacggctc ctcgccgatc agcgtgcggc cggtcaggat
    10741 ggcgtggccc agccccttca tctcccgctg ccgggtgtag gagaaggtgc agctgtccat
    10801 gattttgcgg atgccggtga ggtagcgctc cttgccgctg ccggcgatct gatgctccag
    10861 ctcatagctc ttgtcgaagt ggtcctcgat cgaccgcttg ctgcgaccgg tgacgaaggc
    10921 gatctcggtc agaccggcct cgaccgcctc ctcgacggcg tactgcacca gcggcttgtt
    10981 gaccaccggc agcatctcct tcggcatgga cttggtggcg ggcagaaaac gggtgccgta
    11041 gccggccacc gggaacagac atttcttgat catggggcag tccttggatt ggatcgggtt
    11101 gaggtcaggg cattgatcga tgctgtgcgc ttcgatcagt ttcgccatgg atggttcagt
    11161 tgccgccgct cccagtcgac ggcgctgcga acgatggttt cgaggtcatc gtgacgcggc
    11221 cgccagtcga gcgtggcgag aatgcggcgg ttgtcggccg ccaactgggc cggatcgccg
    11281 gggcgtcggt cgctggtctg caccgcgaag tcgacgccgg tgacctgttt gacgctctgc
    11341 accacctccc tgacgctgag gccatggcca tagccgcagt tgagcagttc gctggtgcca
    11401 ccggcggcca agtgattcag cgcggccaga tgggcgctgg ccagatcttc gacatggatg
    11461 tagtcgcgga tgcaggtgcc atcgggggtg gggtagtcgg tgccgaagat ctgcagggcc
    11521 ggacgcagtc cgagggcggt ctgtgccgcc accttgatca gatgggtggc cgccggggtc
    11581 gattgcccga tccggccacc ggggtcggcg ccggcgacgt tgaagtaacg caggatcaca
    11641 tggcgcaggc cgctgactgc ggcatggtcg atgatcatcc gctcgctgac catcttggaa
    11701 gcaccgtagg ggttgatcgg ctgcagcggg ctctcttcgc tgaccagcgc gctctgcggc
    11761 atgccataga ccgccgcagt ggaagaaaag atcagataac gcggctcatg tttatgcatt
    11821 acttctagca aattcaatac gttgaagctg ttatttcgat aatacttcaa cggattcgcg
    11881 accgattccg gcaccacgat gttggcggcg aagtgcagca ccccctcgaa gcggtgctgg
    11941 cggaacagcc ggtcgagcag tgcggtgtcg cagaggtcac ccaccaccag ttcgccgctc
    12001 agcactgcct gacgaaagcc ggtggagagg ttgtcgagca ccaccacctc atggcccgcc
    12061 tcacccagca accgggtgac atggctgccg atgtaaccgg cgccgccggt aaccaaaagc
    12121 cgcatgctga ctccttgaat gcgcggtgtc gcccgcttgc cgggagactc taccggttaa
    12181 tgttaacctg tttttgacag catgccgggc gtgccgatca gcagcgtgac tgactcagca
    12241 gttcgatgaa ggccgcttca tcgagctggg gcgtgccgag ctgctgcgcc cgggccagtt
    12301 tcgaaccggg attttcgccc accaccagat agtcggtgcg cgccgacagg ctgtcggtca
    12361 gtttggcacc ctgggccgtc agttgcgcct tggccgcatc gcgcgacagg gtggcaaggg
    12421 tgccggtgat caccaccgtc ttgccggtca atggccctgt gccggccgtg gctggtgaga
    12481 tcgccggcca gtggatgccg gcactgcgca gcaccgcgat cacttcaagg ttgtgcggct
    12541 ggcgaaagaa ggtgtggatc tgctcggcca ccaccggacc gacatcggcc acctgttgca
    12601 gggtttcacg atcggcggcc atcaccgcgt cgagctcgcc gaagtggttg gccagggcgt
    12661 tggcggtggc ttcgccgacc tcgcggatgc ccagggcata gagaaacctg gccagcgtgg
    12721 tctgcctgct ggcgtcgatc gccgccacga tccggctggc cgacttttcg cccatgcgct
    12781 ccagcggagt gagttgcgcc gtggtcagcc ggtagaggtc ggccacgctc tccaccagcc
    12841 gctgctcgac cagttggtcg atcagcttct cgccaagccc ctggatgtcg agcgccagtc
    12901 gtgatgcgaa gtggcgaatc gcctccttgc gctgcgctgg acagtagagt ccgccgctgc
    12961 agcgtgccac cacctcgccg ggcggcttga ggatggccga accgcactgc gtgcattgac
    13021 tgggcagctg gatcggtcgg gccgtggcgg gccggcgttc cggcacgcac tgcaccacct
    13081 tgggaatcac atcaccggcg cggcggacga tgacggtgtc gccgatgtgc aggtcgagcc
    13141 gggcgatctc ctccatgttg tgcagcgtgg cgttgctgac ggtcacgccg ccgacgaaga
    13201 ccggtttcag ccgggccacc ggggtcaccg caccggtgcg accgacctgg aagtcgacat
    13261 cgagcagttc ggtcaactcc tcctgggccg gaaacttgtg ggccgccgcc cagcgtggcg
    13321 cccgcgagac gaaaccgagc tgctgctgcc agtcgaggcg atcgaccttg tagaccacgc
    13381 catcgatctc gaagccgagt tcaccccgcc gctgttgcag ccgccggtag tagtcgaggc
    13441 agccctcggc gccctgcacc agtgctgctt cgcggctggt cggcaagccc cactgctgca
    13501 actgctgcat gatggcgctg tggcggtcgg gcagtggccc gccctcgacg aggccgacgc
    13561 cgtagctgca aaagcgcagc ggccgggtcg cggtgatgcg tggatcgagc tggcgcaggc
    13621 tgccggcggc ggcgttgcgg gggttgacga actcccgctc gcccgctgcc cgtgcccgct
    13681 ggttgagctg ttcgaagccg gccagcggca ggtagacctc gccgcgcact tcgagctgct
    13741 cggggtggtc gttgccggca agccgcagcg ggatggccct gatggtgcgc acattgaggg
    13801 tgacatcctc accgcgaaag ccatcgccac gggtggcggc ctgcagcagt tgtccacgct
    13861 ggtagcgcag cgagagcgca acgccgtcga gcttcggctc gcaactgtag tcgatggcga
    13921 catcgaggcc gagccgttcc cgcaggcgct ggtcgaactg tcttgcctca tcggcagtga
    13981 acaggttctc cagcgacagc atcggcagcg catgctgcac ctcggcaaag ctggccagtg
    14041 gtgccgcgcc cacccgctgg gtcggcgagt cgggcgtgac cagctccggg tgcgccgcct
    14101 ccagtgcctg caactcacgc agcagccggt catactccgc atccggaacc gttggttgat
    14161 cgagcacata gtaattgtag ttgtgttgct cgatggcctg gcgcagccgc cgaatctgct
    14221 gcgccggctc ggcagctgct ggtggctcgc tcattgcgct gccgatgcgc tggtggtttt
    14281 ctcgatgaag cggcggatgg tggcgcggta gctgcgcact gcctgggtgt tgaagggctc
    14341 ctgcgtgctg gtgcgcagct cgccatccag cgcgcggcgc agcagctcgg ccatgcggcg
    14401 catctcatcg aaggcatcga gtggccgggc cggaccgggc agggtcagga acagcaccac
    14461 gccaatggtt tcgagctggt cggcctgttc cggatcgaag gtgccgggtt ccagcaggtt
    14521 ggcgacgctg aacagcagtt cacccttgtt gccgccgcgc tgcttgtggc agtgaaagat
    14581 cgcccgttca ccgaattcga gccgttgctg ttgcagcagt tccagcagac gctggccatt
    14641 gaccggcgcc ggcgccagca gatggatcgc cagcacctcg ggttcaatgg gtgcctcctc
    14701 gacctcgtcg gccgtggtgt cacactcatc ctcgaacagc agctgctggg tcatcgggct
    14761 gaccgggcgc tgcggggggg tggagtcagt ggccgattcg gttgccggcg ccggttctgg
    14821 ttcgtgaccg ttgcaggcgc gcaccttggg gcgtgcacca ccgcttggca attcaccgcg
    14881 cagcaggtcg agctcctcca gtgggtcgaa gctgacatcc tcttcgagcc tgacccggat
    14941 cgcgttgcgc cgggcccgcc acacgcgccg gaagccatcg gccagaatga tcagaatcaa
    15001 cagcccgccg atcatgaaca gccagtcgcg catcgacagt tccataggca ccttctcgtt
    15061 attttgcccg cgttcggggg ctcacttcgc cgccattata ccaagggcct gcccgaaaac
    15121 cgccgtggtt caggcgccag ccagcgcctg cgccgcctcg atgtcgaccg tgaccagccg
    15181 tgacacgccg ggctcctgca tggtcacccc cagcagctgg tgggccatct ccatcgcgat
    15241 cttgttgtgg gtgatgtaga tgaactggat gccgtgcgac atctcgcgca ccaggttcac
    15301 atagcggccg acattggcat catcgagcgg cgcatcgacc tcgtcgagca ggcagaaggg
    15361 ggccgggttg agctgaaaga tggcgaagac cagtgcgatg gcggtgagcg ccttctcgcc
    15421 gcccgacagc agatggatgg tgctgttacg cttgcccggc ggctgcgcca tgatggcgac
    15481 gccgctgtcg agcagttcat ccgaggtgag caccagttcg gcatggccgc caccgaacac
    15541 cttcggaaag agcgcctgca ggccccgatt gatctggtcg aaggtgtcac ggaagcggct
    15601 gcgggtttcg cggtcgatct tgcggatggc ctcctgcagc gtttcgagtg cctcgccgag
    15661 gtcggcctgc tgcgcctcca gatacccctt gcgctcgctc tggatgcgat gctcatcgat
    15721 cgcggccagg ttgatcgcac ccagccggtc gatgcgttgc agcagccgct ccagcgcctg
    15781 ttcgagctgc atggcgtcga ggggctcctc gcttcctgca atcaggtcga cgctgctgcc
    15841 gaggctgcgc agctgttcgg ccaacgcatt gtcgtgcagt gccagttcgc gcagttgcag
    15901 acgtttctca tcgacctgct gggtgaggct gcgcagctgg ctgtcgaggt ggcggcgctg
    15961 atcctcctgt tcgcgctgct gccgttccga ctgctcgact gcctgccgcg cggcggccag
    16021 ccgctgttca gccatcagct gcgcggccag cgcctgatcg agctgctgtt gcagcgcctg
    16081 ctgcggttcg accagatcgg caatggccat ctgcagctgc tgccgttgcg cggtggcgcg
    16141 ttcgatctcc tgttcgagcc gcgcactctg tgcctgggct gccttgagtt cagccaggca
    16201 gcgctggcgt tccagcgcca gccgatgctg ctggtcgcgg cagtcacgtt catggctgcg
    16261 cgcctgcgcc agcgcctcgc gcagctgctc ctgctcggcc cgcagccgct gccgctgctg
    16321 ttcgaactcg ccactctgcg cgatggcctg gttcagtgcg gcctgcgtcc gtgcctgccg
    16381 cgcctggtct tcgtcgattt cgcgggttgc gctcgtcagc tcctcgtcga tggcagtgat
    16441 gcggtgctga ctctgctcga tgcgtgcctg acgggcgctc agatcggcct gcagggtggc
    16501 ctgctggcgc cgctgttcgg tggtgcggct ctgggtctgc gcgagctcgg cctcgcactg
    16561 ccgcagatgc cggtcgaact cttcgagctg ctgttgtgca tcctgcaact gctccttgag
    16621 ttgctgctgc tcgcgcttca gctcggtcag ttcgcgggcg cgcagcaggg cgccgccgcc
    16681 ttcgcggtcg tcgggcagtc gctcgatggc gtggcggccg atcagcaggg catcgggggt
    16741 gagaaagaat tcgccggggc ccagttgccc gcgccggtcg agggcgctgg ccagattgtc
    16801 ggcacagtag ccaccatgca gccagtcggc aatttcgtgg ctgctgcgca gccgttcgct
    16861 gagtcggggc cagcgggctg gaggcggttg tgcaggtgcg tgcgctgttg tcagcagcgc
    16921 gaggggaagc tcgaacagcg ccgccatcag ccgttccggt tcgtcggcac gctcggccgg
    16981 caggcagagc gcccggatgc ggtgtgccaa cagccgttcg atcgcccctt cccaacctgc
    17041 ctccacctcg agccgttcgc ccagccgtgg caagcggtcc agttcgaggc gttgcagcag
    17101 ttgggtctgt cgcgggtcga gtcgtccgcg gcccagggca gcctgctgca gcgtttcgag
    17161 cgaactgatg cgcccgctca gctgctggtc gaggcgtcga agctggtcga gccgtggctg
    17221 caaggcgttg cgctgctggc gcagctgctg tcgtcgctgc tcgctcgcct gccgtgccac
    17281 ttcgctcagc cgaatctcgt cggcgagctg gtcgaatctc tgccgcagtg ccggcagtgc
    17341 cgtgtcgtcg atctggcccc gcaggctgtc gcgttcgcca tgcagccgct cgatgcggct
    17401 cttcagccga ggcagttgct gttcgatctg ccggagggtg gcgcgggcgg cctctgtctg
    17461 gcgggccggc agctgcgcct cgcgctggag tcggtcgaac ccctcctgcc actgctgcat
    17521 gcgctgttcg gtggcggcca gctgctgcgc cgcctgctgc tcggcgacct gggcggcttc
    17581 gcttcggggt gtcaggtcct gcagggtgtc gctcagtgtc gtgccgagct gctggctgtg
    17641 ggcctgttgt gcgtgcagtg cctgcatctg tcgttcgttg tcggcgagtg tcgtgtgcag
    17701 ttggcgggcg cgctcctggg catggcggat gccctgttcc aggcggctga tctcggcccc
    17761 ctggcggtag tagtggccct gttcgagatt gaaggcctcg tgcagttcga gatggcgcag
    17821 ccgcaactgc tccagttcgc gttcgagacg gctctgctcg gccagcaggc catcgcggag
    17881 cagactctct ttgctcactt catcgtcgag ccgcagccgc tgctgctcca gcgcgcgcca
    17941 gcgcagcgcg gccagtcgaa tccgctgcga ccgctcttct tctttcagtt cgcggtactt
    18001 ctgggcggcg cgtgcctgcc gctccagggt ctgcaactgg cgctgaagtt cttctttgat
    18061 gtcattgatc cgttgcaggt tctctttggt gtgctgcatc cggttctcgg tttcgcggcg
    18121 ccgctccttg tacttggaga tgccggctgc ctcctcgatg tagacccgca ggtcatccgg
    18181 tcgcgcctcg atcagatgcg agatcattcc ctgttcgatg atggcgtagc tgcgtggacc
    18241 gaggccagtt ccgagaaaaa tgtcgacgat gtcgcggcgt cgacagcgca caccgttcag
    18301 gtagtagctc gactggccgg cacgggtcac cttgcggcgg atcgagattt cggcaaagcg
    18361 ggcatactcc ccgccgaggc tgccggcgct gttgtcgaac agcagttcga tgctggcctg
    18421 gccaaccggc ttgcgatggg tggagccatt gaagatgacg tcggccatct gctcgccgcg
    18481 cagctgtctg gccgagctct cgcccatcac ccagcgcacc gcgtcgatga tgttcgactt
    18541 gccgcagccg ttggggccga ccaccgcact gaggttggtc gcaaaggcga ccgtggtcgg
    18601 atcgacgaag gatttgaatc ccgccagttt gatgctctgc agccgcatgt cggtggttcg
    18661 gtgggtcaaa ggctgcggcc attctaggcg ctgttgcacc gcttcgcacc agcatcctga
    18721 ttttgttgaa gatgttttga tcctccactg agggcgatcc cggttctgga tcttcggtgc
    18781 ggcccaaagg atgcagatcg aagagcgatc cggcgatcgc ggccgaagcc cgcggcagca
    18841 gaccctgttc ctgccggacc atggccgcgg gttcgtgagc gccttcgagg ccctggattc
    18901 atgcgcgacg ggagcagtgc aacaaattgc cgctgtcggc cttacgattt agtatggcgg
    18961 cgacactctg aattggctgg gatgccatgc agcgctttcc cgccgaccgc ctgcgtcacc
    19021 ctctttacaa cgaaaataac gatggagaat cgatatggga cctcttgctg gagtcaagat
    19081 catcgaaatc gccggaatcg gccccggccc cttctgcggc atgatgctgg ccgacatggg
    19141 cgccgaagtg atccgggtcg atcgcgccag cgcgccggcc aatgcgccgc gctccaatga
    19201 cgtgctggcg cggggccgcc gctcgatcgc gctcaacctc aaaaagcccg aagcgatcga
    19261 gacggtgctg aagctgtgcg agcaggccga tgccgtgttc gaggggtttc gtcccggtgt
    19321 cgccgaacgg ctcggcattg gtcccgatgc ctgcatgggt cgcaacccga agctggtcta
    19381 cggccgcatg accggttggg ggcaggatgg ccccatgtcc caggcggccg gccatgacat
    19441 caactacatc tcgctgtcgg gtgcgctgca cgccattggc cccaaggggg gcaagccggt
    19501 gccaccgctc aatctggtgg gcgacttcgg cggcggtggc atgctgctcg ccttcggcat
    19561 ggtctgtgcg ctgctcgaag cgggccgttc cggccgtggc caggtggtgg atgcctcgat
    19621 ggtcgaaggc tccgccgcgc tgatggcgat gatctactcc ttcgccagtg gcggtggcca
    19681 cagcgaggag cgtggcacca acatgctcga tggcggcacc cacttctacg acacctacga
    19741 gacccgggat ggcaagtaca tctcgctggg ctcgatcgag ccgcagttct acgcgctgct
    19801 gatcgagaag gcgggactcg atccacagaa gttcgcgccg cagatggacc gcaacaagtg
    19861 gccggagctc aaggaggagc tgacccgggt gttcaagacc aggagccgcg atgagtggtg
    19921 cgccatcatg gagggcagtg atgtctgctt tgcgccggtg ctgtcgttcc gcgaggcgcc
    19981 cagccatccc cacaacgtcg cccgcaactc ctttgtcgag atcaatggcg tgatgcagcc
    20041 ggcgccctgc ccgaaattca gccgcagcgc gccggagcag ccccggccgc cacgccgggc
    20101 cggtgaggac accgaggcgg tgctgagcga cttcggcttc agcagcggtc agatcgccag
    20161 cctgcgcgcg gcgggtgccc tggcctgagc gaagttgccg tcgactgtgc aattccggca
    20221 atgaacgggg ttgcacagtc gcttcgagtg gacattcagc gcgtggccga tcggtgccgc
    20281 gcttcatcca ctgccaatgg ccgccagcgg caccagggtc atcagtgccc ggtagagcag
    20341 ggcgccatct tccagcacgc cgaaacaggg aaagcagcag tgtgctgccg tcctgccgcg
    20401 cagccccgtg gaagatgcgg cgggcagcgg ttcgcaaacc agcatgtaga gcacgctgtt
    20461 gctcttgacg cgccgcatca actggcggtc gacgctgaac tgctgtgagg cgtagagccg
    20521 tggccgctgc atgaaatcct cccctgcctt cagaccacgc tccagcagcg cgcaggagag
    20581 gtaggcgagc gggaacatct cgggaaaaac cacccggtct tccagttcat tgaagtaatg
    20641 cgtcggatcg acctgggcgc cgagcagaaa attcttgccg ttgccggtca tcgtgaactt
    20701 gcgcttcaca aagcatgcct gacccggcag atggtcgcgg tcagtcaccg ctgacagcgg
    20761 atcgggcagc ggcggagtgc cctctggcag caccagtggc tgccgggact cccgcttgtg
    20821 acccagcagc gccgtgcgtt gacctgctcg cagcagcagg cggttgcgca gcagcgtcgt
    20881 cgggctggtc accagtgtcg aaggcttgat ctcgagcgtc acctcatcac ccggcagcac
    20941 cgggctgacg aaatcgaact gatagttgct gtagcgcagg ccatgctgct cgatcagccg
    21001 cgcttcgtcc gcctgctgtc gatgctggcg cagccggtct tcgatccatg ccgccagttg
    21061 aaagccgagc accaggggcg cgctgaacgg ggtgccggga atctgccacc acagcttctg
    21121 gtcatgaaag gggttgaaat catcggtcga gaagcgcgcg atgtcgatgt gcaactgggt
    21181 gaagcgggtc ggggcggtgg cggtgtgcat cggcgggcac ctgacggcgg atgcggacga
    21241 t
//
LOCUS       DAORCQ010000034        21033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1452_length_21033_cov_5.077414, whole genome shotgun sequence.
ACCESSION   DAORCQ010000034 DAORCQ010000000
VERSION     DAORCQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 21033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21033
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_21033_cov_5.077414"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1340)
                     /locus_tag="PKI23_05335"
     CDS             complement(264..1340)
                     /locus_tag="PKI23_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN36398.1"
                     /translation="MLIFRQLFDQESSTYSYLLGDSTAGEAILIDPVFEQYRRDRALL
                     DELGLRLVQVIDTHCHADHVTAAWLFKQQTGAQIGISAHSGVEGADRPLRHGDQIRFG
                     SRFVSVRETPGHTDGCLTYVLDDERMAFTGDALLVRGCGRTDFQQGDARTMYRSIKTQ
                     IFSLPDTTLLYPAHDYRGITVTTVAEERQFNPRLGGHISEDDFVGYMHNLGLPHPRQI
                     DIAVPANLRCGRPQDDSAPDQGPTWAPLLYTFSGIWEIDPQWLEEHLRAVQIIDVREP
                     DEFTGPLGHIPGARSIPLGQLAERVAEIDRNTPVVTVCRAGGRSAQATVILRRAGIDQ
                     AANLAGGMLRWRSRGFSVVGGFRD"
     gene            complement(1346..2041)
                     /locus_tag="PKI23_05340"
     CDS             complement(1346..2041)
                     /locus_tag="PKI23_05340"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNN36399.1"
                     /translation="MSLHDALTRQIQHLLPGGRITLCRPPLCRDIGLYLLDDQWLSEG
                     LAPEIAQGLMDEPPYWTLCWASGHALAHFILQQPHWARGKRVLDFGAGSGVVAIAAAL
                     AGAARVYACDIDPLAQLAIRTNAEFNGVEVEPITTLDELPEPIDLVTAADVLYDLQNH
                     ALFPRLMQLGEALLLADSRFKQPPTAGFELIDQLEVPPWPDVDSFADFNRVRLYRHQR
                     AGVGHSTPAATRG"
     gene            complement(2038..2706)
                     /locus_tag="PKI23_05345"
     CDS             complement(2038..2706)
                     /locus_tag="PKI23_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNN36400.1"
                     /translation="MSDRLQILTNIRRSLGRDALAPAQQQPLEAALAAPQPNLIPAQT
                     RLNGAALLERFVTKAEAALASVQRLGNLDELPAAIEAYLRQHQLPAALTVPESGLAAL
                     LGPQPLPFALLQRPARSGDAACLTGCFAAMAETGSVVLRSGAELPIGSGYLPDSHLVL
                     LRSEQIVDCYESLWRKVRAELGTMPRSLNWITGPSRSADIGMSMQMGAHGPIRLHLLL
                     LESP"
     gene            complement(2699..4138)
                     /locus_tag="PKI23_05350"
     CDS             complement(2699..4138)
                     /locus_tag="PKI23_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNN36401.1"
                     /translation="MQNHSHEFDNLIGRALDDTQLRQSLSLLRFGLSFTRSRAMAELP
                     EFDQLRDQAVAIKNHTLNHLDFYLERLEQQVKAAGGEVCWAVDAAAASQTILDLCRRH
                     GVRNVTKGKSMMTEEIDLNARLEAAGIQVKETDLGEYVLQLRDEAPSHIVGPAIHLTV
                     DQVADTFHQHHRAHGFEARTTEIPQMMREARTVLRKHFLEADMGITGANFLIAEQGSA
                     MIVTNEGNGDLTAVLPRVHVVVAGIDKVVPTLEDASTLLRLLTRSATGQECAVYTTFF
                     TGAKRSGDTDGPEAFYLVLVDNGRSQMLGSELKEMLRCIRCGACMNHCPVYGEIGGQA
                     YGSVYPGPMGAVLTPALVGLERAHRLPNASTFCGKCEEVCPVRIPLPKLMRHWREQGF
                     ERQLEPLSFRFGLSLWSWVARHPPLYRLLTSLSVKLLNRLGRRQGYLRRLPLGRGWLA
                     WRDLPAPQQQTFFASWEAKQNRGRGASDV"
     gene            complement(4143..4913)
                     /locus_tag="PKI23_05355"
     CDS             complement(4143..4913)
                     /locus_tag="PKI23_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNN36402.1"
                     /translation="MTTPPDTPTVALFVTCLVDLFRPSVAHAAIELLEAAGCRVEVPS
                     AQTCCGQPAFNAGDRRTARRLASQLLEAFAGYDHVVAPSGSCIGMWRQFPDLFAEDDP
                     RHQQALQRQQSCHELSQFLVEVRGYRPPPLDGAPTFTYHDSCAGLRELGIRTQPRQLL
                     AERGATLVEMPNAEVCCGFGGTFCNKFDTISNQMVSHKVEAAVASTAPLLLAGDLGCL
                     LNIAGKVRREGVALECRHFAEVLADQLHRPAIGQPQPE"
     gene            5067..5143
                     /locus_tag="PKI23_05360"
     tRNA            5067..5143
                     /locus_tag="PKI23_05360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5101..5103,aa:Pro,seq:tgg)
     gene            5170..5246
                     /locus_tag="PKI23_05365"
     tRNA            5170..5246
                     /locus_tag="PKI23_05365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5204..5206,aa:Arg,seq:tct)
     gene            5266..5341
                     /locus_tag="PKI23_05370"
     tRNA            5266..5341
                     /locus_tag="PKI23_05370"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5299..5301,aa:His,seq:gtg)
     gene            5390..6043
                     /locus_tag="PKI23_05375"
     CDS             5390..6043
                     /locus_tag="PKI23_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNN36403.1"
                     /translation="MEKRSSQIDRINRWLHCPTPQRWIDAATGQLDLLLIDHANCEKK
                     AASSALNLLFRYVEHPQLMSKMAQLAREELLHFEQVLALMTQRGIHYRHLPAGDYAAL
                     LHQGVRRQEPGRLVDLLIVGAVIEARSCERFARLAPHLDAELGHFYRSLLRSEARHYQ
                     DYLDLARQCAGEPIEARVEEFLQQERRLIERESAQLRFHSGVPLSPDQAVIGKSISQ"
     gene            complement(6010..6738)
                     /locus_tag="PKI23_05380"
     CDS             complement(6010..6738)
                     /locus_tag="PKI23_05380"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNN36404.1"
                     /translation="MASLFISDLHLQPENPSVTAAFRRFLDETASQAEALYILGDLFD
                     DWVGDDDDNPFYLAVLDDLRRLHDRGVALFFLHGNRDFLIGADFASTTGATLLPDPVR
                     IELHGHALLLAHGDGFCTQDQEYMAFRQLLRDPQWQRQALEKPLAERRAIAAQLHQIS
                     QERNQQKASEIMDVTPDEVVRVMEENRASLLIHGHTHRPDIHPLRLAHGDAVRLVLGD
                     WARSGWFARLDESGYWLIDFPITA"
     gene            complement(6747..7247)
                     /locus_tag="PKI23_05385"
     CDS             complement(6747..7247)
                     /locus_tag="PKI23_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNN36405.1"
                     /translation="MTQVVLHTNHGQITLALDAEAAPLTVANFVDYVKSDFYNGTLFH
                     RVIDNFMIQGGGMDAEMKQKPTRDPVDNEANNGLKNLRGTIAMARTSDPHSATAQFFI
                     NVTDNAFLDHTAPTRQGWGYCVFGKVTEGLEVVDAIKVVKTGMKGMHGDVPLEAVLIE
                     RAEIVG"
     gene            7378..8760
                     /gene="cysS"
                     /locus_tag="PKI23_05390"
     CDS             7378..8760
                     /gene="cysS"
                     /locus_tag="PKI23_05390"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNN36406.1"
                     /translation="MHFTLHNSLSGHKEPFQPLQPGKIGLYVCGMTVYDYCHLGHARV
                     MVAFDVIVRYLRSIGYQVSYIRNITDLDDKIFARAAENGEPFTALTDRFITAMHEDEA
                     ALGVLPVDSEPRATHHIDQIIALINRLIERGYGYLASNGDVYYRVRRFADYGRLSKRN
                     LEELQVGARIEVGEAKEDPLDFALWKAAKPGEASWDSPWGAGRPGWHIECSAMSICAL
                     GETFDMHGGGPDLKFPHHENEIAQSEGATGKPFARYWLHVGPVRVNDEKMSKSLGNFF
                     TIRQLLQQHQPEVIRYLLLASHYRSPINYSEESLRGARQALERFYTALRGQSVAAVLT
                     MADNDYAERFHAAMRDDFNTPEALAVLHELTRALNRAQDDSMAAGLAAQLRQLGGLLG
                     LLQQPPEQFLQGSPRDGLDDSEISALVEARNQARRERNWPLADQLRAQLEQAGILLDD
                     GRDGTQWRRA"
     gene            complement(8764..9852)
                     /gene="queG"
                     /locus_tag="PKI23_05395"
     CDS             complement(8764..9852)
                     /gene="queG"
                     /locus_tag="PKI23_05395"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNN36407.1"
                     /translation="MKPPDLPQDYHQLAARIKAWGVELGFQAVGIAGIELPEDEQHLL
                     DWLQRGDHGDMAYMAQHGSRRGRPAELLPGTLRVISLRLDYLPPGVALQQTLQQPERA
                     YISRYALGRDYHKLMRKRLAQLAERISSAIPAHHYRVFVDSAPVLEKALAVQAGLGWI
                     GKNTLLLNRHAGSWFFLGEIYTNLPLPLDLPTGKGHCGSCRACLDICPTQAFRAPYQL
                     DARRCISYLTIESKGPIPLELRPLIGNRVFGCDDCQLVCPWNRYARPTNERDFLPRHG
                     LEQAQLLELFAWDEASYLARSEGSPLRRIGHAGFLRNLAVALGNLLRQTSSPLLQQQI
                     IQQLNVRSDHPSALVREHVQWALAQQQR"
     gene            9898..11424
                     /locus_tag="PKI23_05400"
     CDS             9898..11424
                     /locus_tag="PKI23_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNN36408.1"
                     /translation="MVDPISSFRDTLPTLLYTAAQSRQLDRLAQQLPGIDGSVLMRRA
                     GSAAFDVLRSHWPQARRLLIFCGGGNNGGDGYVLAELAQRAGFSVTLVALVAAEQLRG
                     EALVACRNAVAAGVEPLPFAAERLVDTQHDLLVDALLGTGLSGEPRAEYRAVIDWINA
                     SALPVLALDLPSGLDADTGRVWGAAVRADLTIAFIAAKRGMLTGAGPDHCGLLGFADL
                     ELPGALHQKLESEPVLRLTSTLLPQWLPRRPRSAHKGMFGHLLIIGGNHGYGGAVSIA
                     AEAALRAGAGLVTIATRGAHLSALLARRPEAMVRAVESGQDLEPLLALPTVILLGPGL
                     GQDAWAEQMVQRVLGSDLPLVVDADGLNLLARRATPVHRENWILTPHPGEAARLLGCD
                     SREVEADRFAAVQQLAERHGGVALLKGAGTLVASAAVGGGIALCAAGNPGMASGGMGD
                     LLSGLIAALLAQQLSLVDAARAGVLLHAMAGDEAAGGNERGLLATDLLAPLRRWVNPL
                     "
     gene            11437..11955
                     /gene="tsaE"
                     /locus_tag="PKI23_05405"
     CDS             11437..11955
                     /gene="tsaE"
                     /locus_tag="PKI23_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNN36409.1"
                     /translation="MSDSTTILTLLLPDEVATLRLGGVLAEVVAAGILFLRGDLGSGK
                     TTLSRGLLHGLGHQGAVKSPTYTLVEPYLLSELALYHFDLYRFADEKELEYLGVRDYF
                     REDSLCLIEWPERGEPLLPSPDLEIRLKWIEKGRWCEIEGCSDRGRMWLAQLRCNPVV
                     QALAVAAVDDSA"
     gene            11883..13337
                     /locus_tag="PKI23_05410"
     CDS             11883..13337
                     /locus_tag="PKI23_05410"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNN36410.1"
                     /translation="MVGAVALQSCGAGIGRGRSRRLSLTIRFGSLLCLLVFLLAQSAS
                     GAAILKSAKVENSGESSRLLLDFSAGVTHKFFTLSAPDRLVIDLTETQLGAALDRLDL
                     AATPIARIRTSHQPQSARLVFDLKQRTPVRVYLTAAEGAATQQRLVVELGAQKKTAKP
                     PLPAAAATARSVKPPVAEKPRSPSVTQPPLVKGSEKPSPAASQSARVTAVAPNVKSVQ
                     QLNHERRDLVIAIDAGHGGDDPGALGPGGIREKEITLAIARQLHGLLSQEPGFKPVLI
                     RSGDRFVPLRQRTEIARKHKADLFISIHADSFSGSDARGGSVYTLSQRGASSESARWL
                     ADQENKADLVGGVGLDQRDGMVAQVILDLYMTATLNTSNELASELLRGLNKVGPLHRQ
                     QVEKAGFMVLKSPDIPSVLVETGFISNPSEARLLNTRQHQQRLAQSLRGAIVSYYSRR
                     PPEGTMLAWRKRGGDLSASPAVAQNESSELPVRR"
     gene            complement(13372..14037)
                     /locus_tag="PKI23_05415"
     CDS             complement(13372..14037)
                     /locus_tag="PKI23_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNN36411.1"
                     /translation="MSERQYRLWPWPIRLLHWLLATSFVLAYLSGEEALWLHALAGHL
                     IVLLIGLRLLGGLFSTTPAALDQLWITPTAVKAHLAALLALRPLPHHGHHPLGALNIL
                     ALFSILLLLCVTGMALLALDHGKGWLMGFFPAGMVWHKWLLAGSHELLAQFSLLLVLL
                     HVVGVLGQSLIDRRNLSRSMLGGIMITLHRSRGWKSLWRRVAILLLVLLPSLIELLAL
                     RVL"
     gene            complement(14168..14770)
                     /locus_tag="PKI23_05420"
     CDS             complement(14168..14770)
                     /locus_tag="PKI23_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNN36412.1"
                     /translation="MLLAHTVEIFTRPNQAWQSIHDETESPLHHLFNHLIWLALLPPL
                     CWYIGTTTFGWSIGSGPATKLTSQSALIIALLFYGAILAMVMLLGALIHWMAQTYGAN
                     SSFARSLKLAGYTVTPLLLSGLLGIYPALWFDLVVGLVAIGYTVYLLYQGTPIMMEIS
                     PERGVLFSSAVIAVGLVMVVALMGATVILWSMGAAPQFVS"
     gene            complement(14894..16258)
                     /locus_tag="PKI23_05425"
     CDS             complement(14894..16258)
                     /locus_tag="PKI23_05425"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNN36413.1"
                     /translation="MSLIETLLTWLQSHPGWTLAAIFIVALAESLAIVGLLVPGVVLL
                     IGISVLAGSGTVEAAPLLLAGMIGAILGDGISFWIGHHFHQHLREFGPFRRHPEWIDN
                     GEAFFYRHGGKSIVLGRFVGPIRPFIPMVAGMLDMPVGRFILTNLFSALLWAPVYLLP
                     GYLLGSSLQQSEAQIADAMQLGIVLLLVGSVALYGLRFGYLALSPGHLFYRFCQRQLT
                     STRIGALLWRWRASGGRADGVFPLASLALALLLAMLFALQGVLLNGHDRAVADFFSSL
                     RHPAITLLLLPLKLLAERPHLAGQAGLLAIWLLARRGWHALGFWFALLLLTLQLANRQ
                     LPLATLTAPLGFAAYLIAQSWTPPHRFVIYTGMAALISLFGISQLYFEAPPFTTVIKE
                     LLVGLLIANLMTLFCHRHHEELALRGTLPGWSWSSASLMISVVYLLQHWSHASARMVG
                     SGWL"
     gene            16391..17653
                     /locus_tag="PKI23_05430"
     CDS             16391..17653
                     /locus_tag="PKI23_05430"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNN36414.1"
                     /translation="MNQPLAQQMQQIGLRAREASRTLARASTGDKNRALIATAEALQQ
                     HRTTLLHANGHDLEAARARQLEPALIERLTLNDARIDAMIESLHQVAALADPVGAVTD
                     LRRMPSGIEVGRMRVPLGVIGIIYESRPSVTAEAASLCLKSGNAAILRGGSESHHSNR
                     VIAQAIQHGLATALLPDHAIQVLESTDRAAVGHLITMPEHVDVIIPRGGRGLIERISQ
                     EARIPVIKHLDGICHLYVDAHADLEMALKLAVNAKTHRYGVCNAMETLLVCAAVAERF
                     LPLITDALKVKGVELRGCTETRRWVPDCTPACDEDWITEYLAPILSIRVVAGLDEAID
                     HINRHGSHHTDGIITQRLDHARRFITEVDSSSVMVNASTRFADGFEYGLGAEIGISTD
                     KLHARGPVGLEGLTSQKYVVLGDGHIRQ"
     gene            17650..18354
                     /gene="nadD"
                     /locus_tag="PKI23_05435"
     CDS             17650..18354
                     /gene="nadD"
                     /locus_tag="PKI23_05435"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNN36415.1"
                     /translation="MSGLLAEQPRNRAVGFMGGTFDPVHHGHLRCALEIKELLALDEM
                     RLIPCGDPYHRSAESLPAAVRVQLLEAALVGNRALRIDRRELARTGPSYSHDTLLSLR
                     GELGEQTALCMVVGSDAYQDLDRWHRWETLLDLGHLLVVERPGWQPQERPALKALRGR
                     CETDRIDRLRNRPAGAMLFLSLPRLEISSTRIRALIAAGRSPEYLLPEAVWQMIRQQQ
                     LYGYRSTDTDNSPDER"
     gene            18344..18688
                     /gene="rsfS"
                     /locus_tag="PKI23_05440"
     CDS             18344..18688
                     /gene="rsfS"
                     /locus_tag="PKI23_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNN36416.1"
                     /translation="MNVETLKERLLTVLDERKAEQIVCLDVHALTSITDWMIIATGTS
                     SRHTKAVAEYLVQEAKNLGHQPTGVEGLQSGEWVLVDLDRIIVHIMLAETRRLYDLER
                     LWRQTPLMPAQD"
     gene            18695..19162
                     /gene="rlmH"
                     /locus_tag="PKI23_05445"
     CDS             18695..19162
                     /gene="rlmH"
                     /locus_tag="PKI23_05445"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNN36417.1"
                     /translation="MRIRLLAAGNRPPAWIKSGYEEYISRLRPYCRFELIEIPLPSRG
                     KSLSAQQARRIEGERMLAALGERDRLIALAIEGEAWSTAQLSARLGEWERAGDPLALA
                     IGGPDGLDDALLARAQRRWSLSALTLPHLLVRVIVTEQLYRAHALRDNHPYHR"
     gene            19169..21013
                     /gene="mrdA"
                     /locus_tag="PKI23_05450"
     CDS             19169..21013
                     /gene="mrdA"
                     /locus_tag="PKI23_05450"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNN36418.1"
                     /translation="MSPSPVFKDHEQERRLVQRRLLWSVLLLLLCMALLLGRIYTLQV
                     VKHEEFHTLSDKNRIRLQPIPPTRGLIYDRNQQLLAHNRASFNLTLVKESIADLPQTL
                     DRLTTLLKLTDEELNRFRKRLERSRSFDLVPLRDNLTEEEIAILALERHRLPGVEVSA
                     QLVRHYPFGPLFAHPLGYVARINESELKEVDPVNYSGTEYIGKGGVERQNEALLHGQV
                     GYQNVEIDARGRVIRVLNSTAAQNGADLTLYLDSHIQQVAEEALTGWRGAAVAIEISS
                     GGVLAMVSTPSYDPNLFVTGIDARRYGELQKSTDLPLLNRAVRGRYPPGSTVKPFVAL
                     AGLQHQVINFDSTVADPGWYQLPGNEHKYRDWKKWGHGYRVGLRQAIVESCDTFFYDL
                     AYRLNIDRLSDTLGRFGFGALSGAEFPGELPGILPSRAWKQARYRANWFPGETVITGI
                     GQGYMTVTPLQLAAATATLANRGVMVRPRLVKSISPVDSAALLPSAPPPIQLAQRGYW
                     DFVIGAMEGVVHDPTGTAKRIAVDAPYRIAGKSGTAQVVGIAQDEEYDAGKISKKNWD
                     HALFIAFAPAEAPRIAVAVVVENGGSGSGTAAPIARRILDAYLLPRQP"
BASE COUNT         3767 a         6429 c         7049 g         3788 t
ORIGIN      
        1 gtttccgctc cgttttggca gaaaaccgta acgggaaacc gtggtctgtc cccaattctt
       61 tccaattctt tcaattcttt gcgtggtctg tccccaattc tttgtgtccc caattctttg
      121 tgtccccaat tctttgcaat tctccttgga agacgaaatt tcgggggagg ctcaatccgt
      181 ttcagctggg atcgatacgc caaagaaagc ggtcgtcttt tcggtgcatt gctgcacgat
      241 gccgcgttgc tcgactccgc cgttcaatcc ctgaacccac ccaccacgct gaagccacgc
      301 gagcgccagc gcagcatgcc gccagccagg ttggccgcct gatcgatgcc ggcgcggcgc
      361 aggatgacgg tggcctgggc cgaccgtccg ccggcgcggc agacggtgac taccggcgtg
      421 ttgcggtcga tctcggcaac ccgctcggcc agttgaccga gcggaatcga gcgggcaccg
      481 ggaatgtggc cgagtggacc ggtgaattca tcgggttcgc gcacgtcgat gatctgcacg
      541 gcgcgcagat gctcttcgag ccactgcggg tcgatctccc agatgccgct gaaggtgtag
      601 agcagtggcg cccaggtcgg gccctggtcc ggcgcgctgt catcttgcgg ccggccgcag
      661 cgcaggttgg ccggcacggc gatgtcgatc tgtcggggat ggggcaggcc gaggttgtgc
      721 atgtagccga cgaagtcatc ttcgctgatg tggccgccca gtcgtgggtt gaactgtcgc
      781 tcttcggcga cggtggtgac ggtgatgccg cggtagtcgt gggcggggta gagcagggtg
      841 gtgtcgggca gcgagaagat ctgggtcttg atcgagcggt acatggtccg ggcatcgccc
      901 tgctggaagt cggtgcggcc acagcctcgc accagcagtg catcgccggt gaaggccatg
      961 cgttcgtcat cgagcacata ggtcaggcag ccgtcggtgt ggcccggggt ctcacgaacc
     1021 gagacgaagc ggctgccgaa gcggatctgg tcgccatgtc gcagtgggcg gtcggccccc
     1081 tcgacgccgc tgtgggcgct gatgccgatc tgggcgccgg tctgctgctt gaacagccag
     1141 gcggcggtga catggtcggc atgacagtgg gtgtcgatga cctgcaccag ccgcaggccg
     1201 agttcatcga gcagcgcgcg gtcgcggcgg tactgctcga acaccgggtc gatcaggatc
     1261 gcctcaccgg cggtgctgtc gcccagcagg tagctgtagg tcgaggattc ctggtcgaac
     1321 agttgtctga agatcagcat tggcgtcatc cccttgtggc ggctggagtt gagtggccga
     1381 cgccagcgcg ctgatggcga tagaggcgca cccggttgaa gtcggcgaag ctgtcgacat
     1441 cgggccaggg cggcacttcg agttggtcga tcagctcaaa cccggcggtg ggcggctgct
     1501 tgaagcgcga atcggccagc agcaacgctt cgccaagctg catcagccgt ggaaagagcg
     1561 catggttctg caggtcgtag agcacatcgg cggcagtcac cagatcgatc ggctccggca
     1621 gttcatcgag ggtggtgatc ggttcgacct cgacgccgtt gaactcggca ttggtgcgga
     1681 tcgccagctg cgccagcgga tcgatgtcgc aggcatagac ccgtgcggcg ccggccagtg
     1741 cggcggcgat ggcgacgacg ccggagccgg caccgaagtc gagcacccgc ttgccccgcg
     1801 cccagtgcgg ttgttgcaga atgaagtggg ccagcgcatg gccgctggcc cagcagagcg
     1861 tccagtaggg cggttcatcc atcaggccct gggcgatctc cggcgccagc ccctcgctca
     1921 gccactggtc gtcgagcaga tagaggccga tgtctcggca gagcggcggg cggcagaggg
     1981 tgatgcggcc gccgggcagc aggtgctgaa tctgccgggt cagggcgtcg tgcagactca
     2041 tggcgactcc agcagcagaa ggtgcaggcg gatcggaccg tgggcgccca tctgcatgct
     2101 catgccgatg tcggcactgc gtgacgggcc ggtgatccag ttgaggctgc gtggcatcgt
     2161 gccgagttcg gcgcgcacct tgcgccacag gctctcgtag cagtcgacga tctgttcact
     2221 gcgcagcagc accagatggc tgtcgggcag gtagccgctg ccgatcggca gttcggcgcc
     2281 ggagcgcagc accacgctgc cggtctcggc catggcggca aagcagccgg tcaggcaggc
     2341 ggcatcgccg ctgcgcgccg ggcgttgcag cagtgcaaag ggcagtggct ggggtccgag
     2401 cagggcggcc aggcccgatt cgggcacggt cagcgcggcg ggcaactgat gctggcgcag
     2461 gtatgcctcg atggcggcgg gcagttcatc caggttgccg agccgctgca ccgaggccag
     2521 tgctgcttcg gccttggtga cgaagcgttc cagcagtgcg gcgccgttga ggcgggtctg
     2581 tgccggaatc agattgggct ggggtgcggc cagtgccgct tcgagcggct gctgctgcgc
     2641 tggtgccagc gcatcgcggc ccagactgcg gcggatgttg gtcagaatct gtaaacggtc
     2701 agacatcgga cgctcctcgg ccgcggttct gtttggcctc ccacgaggca aagaaggtct
     2761 gctgttgtgg cgccggcagg tcgcgccagg ccagccagcc gcggccgagt ggcaggcggc
     2821 gcagatagcc ctgccggcgg cccaggcggt tcagcagctt gacactgagc gaggtgagca
     2881 gccggtagag cggcggatgg cgcgccaccc agctccagag cgacaggccg aagcggaacg
     2941 acagcggttc gagctggcgc tcgaaaccct gctcgcgcca gtggcgcatc agcttgggca
     3001 gcgggattct gaccgggcag acctcttcgc acttgccgca gaaggtcgag gcattcggca
     3061 ggcgatgggc ccgttcgagg cccaccagtg ccggcgtcag cacggcgccc atcgggccgg
     3121 ggtagaccga gccgtaggcc tgcccgccaa tttcgccgta gaccgggcag tggttcatgc
     3181 aggccccgca gcggatgcag cgcagcattt ccttcagttc gctgccgagc atctggctgc
     3241 ggccgttgtc gaccagcacc agatagaagg cctcgggacc gtcggtgtcg ccactgcgtt
     3301 tggcgccggt gaaaaaggtg gtgtagaccg cgcactcctg gccggtggcc gaacgggtca
     3361 gcagccgcag cagggtcgag gcatcctcca gcgtcggcac caccttgtcg atgccggcca
     3421 ccaccacgtg gacccgtggc agcacggcgg tcaggtcacc attgccctcg ttggtgacga
     3481 tcatcgcgct gccctgttcg gcgatcagaa agttggcgcc ggtgatgccc atgtcggctt
     3541 cgagaaaatg cttgcgcagc acggtacgcg cctcgcgcat catctgcgga atttcggtgg
     3601 tgcgcgcttc gaagccgtgg gcacggtgat gctggtggaa ggtgtcggcc acctgatcga
     3661 cggtgaggtg aatcgccggc ccgacgatgt ggctgggggc ttcgtcgcgc agttgcagca
     3721 catattcgcc gaggtcggtc tccttgacct ggatgccggc ggcctccaag cgggcattga
     3781 ggtcgatctc ctcggtcatc atcgacttgc ccttggtgac gttgcgcacg ccgtggcgtc
     3841 ggcagaggtc gaggatggtc tgactggccg ccgcggcatc gaccgcccag cagacctcac
     3901 cgccggccgc cttgacctgc tgctcgagcc gttcgagata gaaatcgaga tggttcagcg
     3961 tgtgattttt gatcgccacg gcctggtcac gcaattggtc gaactccggc agttcggcca
     4021 tggcgcggct gcgggtgaac gacaggccga agcgcagcag gctgagcgac tggcggagct
     4081 gcgtgtcatc gagcgcccgg ccgatcaggt tgtcgaactc atggctgtgg ttctgcatcc
     4141 tcttactccg gttgcggttg gccgatggcc ggccgatgca gttggtcggc cagcacctcg
     4201 gcaaaatgac ggcactccag cgcgacacct tcgcgccgca ccttgccggc gatgttgagc
     4261 aggcagccca gatcgcctgc cagcagcagc ggcgcggttg aggccacggc ggcctcgacc
     4321 ttgtggctca ccatctggtt ggaaatggtg tcgaacttgt tgcagaaggt gcctccaaaa
     4381 ccgcagcaga cctctgcgtt cggcatctcg accagggtcg caccgcgctc ggccagcagt
     4441 tgtcgtggct gcgtgcgaat gcccagttcg cgcaggccgg cgcagctgtc gtgataggtg
     4501 aaggtgggcg cgccatccag cggcggtgga cgatagccgc gcacttcgac cagaaactgc
     4561 gacagttcgt ggcagctctg ctgtcgctgt agtgcctgct ggtgacgcgg atcatcctcg
     4621 gcaaacagat ccggaaactg tctccacatg ccgatgcagg agccggaggg ggccaccaca
     4681 tggtcgtagc cggcaaaggc ctccagcagc tggctggcca gccggcgtgc ggtgcggcgg
     4741 tcgccggcat tgaacgctgg ttgaccgcag caggtctgtg cgctgggcac ctcgacccgg
     4801 caaccggcgg cttcgagcag ttcgattgcg gcatgggcca cgctggggcg gaacaggtcg
     4861 accagacagg tgacgaagag cgccaccgtc ggggtgtcgg ggggtgttgt catcgatgtc
     4921 agcctcaaat tgccgctggt cagcggctat cctcccactg tgcagatggc aagtcgatcg
     4981 ggttgacgct gttttgtccg gcattgacgc aacaggggtc gctgcgtatg attcgcgctc
     5041 ttcaacgatc ggcaagcgtt gagtttcggg gtatagcgca gtctggtagc gcgcctgctt
     5101 tgggagcagg atgtcgggag ttcaaatctc tctaccccga ccaattttcc gcagaggtcc
     5161 gtgtcaggag cgcctgtagc tcaatcggat agagcaacgg ccttctaagc cgtaggttgc
     5221 aggttcgatt cctgccgggc gtgccacgcg gattgcagtt ctatggtggg cgtagctcag
     5281 ttggtagagc acaggattgt gattcctgtg gtcgtgggtt caatccccat cgcccacccc
     5341 attttccacc tcagatcgcc ttcgtcattg ctcaatcgct ggaatggctt tggaaaagcg
     5401 cagcagccag atcgatcgga tcaaccgttg gctccactgt ccgaccccgc aacgctggat
     5461 cgatgccgcc accgggcagc tcgaccttct gctgatcgac catgccaact gcgaaaagaa
     5521 agccgcttcg agcgcgctca atctgctgtt ccgttatgtc gagcatccgc agctgatgag
     5581 caagatggcg cagttggcac gtgaggagct gctgcacttc gaacaggtgc tggcgctgat
     5641 gacgcagcgt ggcatccact accggcatct gccggctggt gactatgccg cactgctgca
     5701 tcagggcgtg cgacggcagg agccggggcg actggttgac ctgctgatcg tgggtgcggt
     5761 gatcgaagcg cgctcctgcg aacgctttgc ccgcctggcc ccccacctcg atgctgagct
     5821 gggccacttc taccgttcgc tgctgcgttc cgaggcgcgg cactatcagg actatctcga
     5881 tctggcccgc caatgcgccg gcgagccgat cgaggcgcgt gtcgaggagt tcctgcagca
     5941 ggagcgtcgt ctgatcgagc gtgagtcagc gcaattgcgc tttcacagcg gtgtgccact
     6001 gtcgccggat caggcggtga tcgggaagtc gatcagccag tagcccgatt catcgagccg
     6061 ggcgaaccag cccgagcgcg cccagtcgcc caataccagc cgcactgcat cgccatgcgc
     6121 cagccggagc gggtggatgt ccggccggtg ggtgtggcca tgaatcagca agctggcccg
     6181 attctcttcc atcaccctga ccacctcgtc cggtgtgaca tccatgatct cgcttgcctt
     6241 ctgctggttg cgctcttggc tgatctgatg gagctgggca gcgatggcac gccgctcggc
     6301 cagcggtttc tccagcgcct gccgctgcca ctgcggatcg cgcagcagct ggcgaaacgc
     6361 catgtactcc tgatcctggg tgcagaagcc gtcgccgtgg gccagcagca gcgcatggcc
     6421 gtgcagttcg atccgaaccg ggtcgggcag cagggtggcg ccggtggtgc ttgcaaaatc
     6481 ggcaccgatc agaaaatcgc gattgccatg cagaaagaac agggcaacgc cgcgatcgtg
     6541 cagccgccgc aggtcgtcga gcacggccag atagaagggg ttgtcatcgt catcgccgac
     6601 ccagtcatcg aacaggtcgc ccagaatgta gagcgcttcg gcctggctcg cggtctcatc
     6661 gagaaagcgc cggaacgccg ccgttacgct ggggttttcc ggttgcagat gcaggtcgga
     6721 gatgaagagg ctggccatcg gcccggttat ccgacgatct cggcccgctc gatcagcacc
     6781 gcttcgagcg gcacatcgcc atgcatgccc ttcatgccgg tcttgaccac cttgatggca
     6841 tcgaccacct ccagaccttc ggtcaccttg ccgaagacac agtagcccca gccctgtcgg
     6901 gtcggcgcgg tgtggtcgag aaaggcgttg tcggtgacat tgatgaagaa ctgggcggtg
     6961 gcggagtgcg gatcgctggt gcgggccatg gcgatggtgc cacgcaggtt tttcagtccg
     7021 ttgttggctt cgttgtcgac cggatcgcgg gtcggtttct gtttcatctc ggcatccatg
     7081 ccaccgccct ggatcatgaa gttgtcgatc acccggtgga acagggtgcc gttgtagaag
     7141 tcgcttttga catagtcgac gaagttggcg acggtcagcg gtgccgcttc ggcatcgagg
     7201 gccagggtga tctgaccatg gttggtgtgc aggacgactt gggtcatggc aatggttccg
     7261 cgcagatgga gggattgacg taagtcggcc gacggctggg cgtctgccgg gcgcatggag
     7321 tgcgacgaag ccacgtataa taaccgccta cttgttacgc accaacctgg ccaaccgatg
     7381 cacttcaccc tccacaattc gctgagcggg cacaaagagc cgttccaacc actgcaaccg
     7441 ggcaagatcg gcctctatgt ctgcggcatg accgtctatg actactgtca tctgggccat
     7501 gcccgggtga tggtggcctt tgatgtgatc gtgcgttacc tgcgcagcat cggttaccag
     7561 gtgagctaca ttcgcaacat caccgatctg gatgacaaga tcttcgcccg tgccgccgaa
     7621 aatggtgaac ccttcacggc gttgaccgac cgcttcatca ccgcgatgca tgaagacgag
     7681 gccgcgctcg gcgtcctgcc ggtcgacagc gagccgcgcg ccacccacca catcgatcag
     7741 atcatcgcgc tgatcaaccg gctgatcgag cggggctatg gctatctggc cagcaacggc
     7801 gatgtctact atcgggtgcg ccgctttgcc gattatggcc ggctgagcaa gcgcaacctc
     7861 gaggagctgc aggtgggtgc ccgcatcgag gtgggcgagg ccaaggagga tccgctcgat
     7921 ttcgctctgt ggaaggcggc caagccgggc gaagccagtt gggattcgcc ctggggtgcc
     7981 ggtcgtccgg gctggcacat cgaatgttcg gcgatgtcga tctgcgcgct gggtgagact
     8041 ttcgacatgc acggtggcgg gcccgatctg aaatttccgc accatgagaa cgagattgcc
     8101 cagtcggaag gtgcgaccgg caagcctttt gcccgctact ggctgcatgt cggtccggtg
     8161 cgggtcaatg acgaaaagat gtcgaaatcg ctcggcaatt tcttcaccat ccggcagttg
     8221 ctgcaacagc atcagcccga ggtgattcgc tatctgctgc tggccagcca ctaccgcagc
     8281 ccgatcaact acagcgaaga gtcgctgcgc ggtgcgcggc aggcgctgga gcgcttctac
     8341 accgcgttgc gcggtcaatc ggttgcagcc gtgctgacga tggccgacaa cgactatgca
     8401 gaacgttttc atgccgcgat gcgcgacgac ttcaacacgc ccgaggcgct ggcggtgttg
     8461 cacgaactga cccgggcgct gaatcgcgcg caggatgaca gcatggcggc ggggctggcg
     8521 gcgcagttgc ggcagttggg cggactgctc ggcctgctgc aacagccgcc tgagcagttt
     8581 ctgcaaggca gcccgcggga tgggctcgat gacagcgaga tcagcgcgct ggtcgaggcg
     8641 cgcaaccagg cgcgacgcga gcgcaactgg ccgctggccg atcagcttcg tgcccagctc
     8701 gaacaggcgg gcatcctgct cgacgatggc cgcgacggca cccagtggcg acgcgcctga
     8761 ggttcagcgc tgctgctgtg ccaaggccca ctggacatgt tcgcgcacca gcgcgctcgg
     8821 atgatcgctg cgtacattca gttgctggat gatctgctgc tgtagcaacg gcgatgaggt
     8881 ctggcgcagc aggttgccga gcgcaaccgc cagattgcgc aaaaagccgg catggccgat
     8941 ccggcgcagc ggtgaacctt cgctgcgggc caggtagctg gcctcatccc aggcgaagag
     9001 ctcgagcagc tgggcctgct ccagtccatg gcgcggcaga aagtcacgct cgttggtggg
     9061 ccgcgcatag cggttccacg ggcagaccag ctggcagtca tcgcagccaa agacccgatt
     9121 gccgatcagt ggccgcaatt cgagcggaat cggacccttc gattcgatgg tcagatagga
     9181 gatgcagcgg cgcgcatcga gctgataggg cgcgcgaaag gcctgggtcg ggcagatgtc
     9241 gaggcaggca cgacagcttc cgcagtggcc cttgccggtg ggtaggtcga gcggcagcgg
     9301 caggttggtg tagatctcgc caagaaagaa ccaggagccg gcatggcggt tcaacagcag
     9361 cgtgttcttg ccgatccagc ccagcccggc ctgcaccgcc agagccttct ccagcactgg
     9421 cgcgctgtcg acgaagaccc gatagtggtg tgccgggatg gcgctgctga tccgttccgc
     9481 caattgggcg agccgcttgc gcatcagctt gtggtagtcg cgtcccagcg catagcgcga
     9541 gatgtaggcc cgctccggct gctggagtgt ctgttgcagc gccacgccgg gtggcaggta
     9601 gtcgagccgc agcgagatca ccctcagcgt gcccggcagc agttcggccg ggcgaccgcg
     9661 gcggctgccg tgctgggcca tgtaggccat gtcaccatga tcgccgcgtt gcagccagtc
     9721 gagcaggtgc tgttcatctt caggcagttc gatgccggcg atgccgacgg cctgaaaacc
     9781 gagttcaacg ccccatgcct tgatccgggc ggcaagctga tggtaatcct gaggcaggtc
     9841 gggtggcttc attcgttata attctcgccc caactgactg ccaaagcgag cctgccgatg
     9901 gttgatccga tctcctcatt ccgtgacacc ctgccgacgc tgctctatac ggcagcgcag
     9961 tcacgccagc tcgatcggtt ggcacagcag cttcccggca tcgatgggtc ggtgttgatg
    10021 cggcgcgccg gcagtgccgc cttcgatgta ttgcgcagcc actggccgca ggcgcggcgg
    10081 cttttgatat tctgcggcgg cggcaacaac ggtggcgatg ggtatgtgct ggccgaactg
    10141 gcgcagcgtg cgggtttcag cgtcaccttg gtggcgttgg tggcagcgga acagttgcgg
    10201 ggcgaagcgt tggtggcctg ccgcaacgcg gtggctgccg gcgtcgaacc gttgcctttc
    10261 gcagcggaac gactggtcga cacgcaacat gatctgctcg tcgacgcgct gttgggcacc
    10321 ggcctcagcg gcgagccgcg cgccgagtat cgcgcggtga tcgactggat caacgcgagc
    10381 gcgctgccgg tgctggcact ggaccttcct tccggactcg atgccgacac cggccgggtc
    10441 tggggcgccg ccgtgcgcgc cgacctgacc attgccttca tcgccgccaa gcggggaatg
    10501 ctgaccgggg cgggtcctga ccactgcggc ctgctcggct tcgccgacct cgaattgcct
    10561 ggagcgctcc atcagaagct ggagagcgag ccggtgctgc ggctcacatc gaccctgttg
    10621 ccgcagtggc tgccgcgccg gccgcgcagc gcccacaagg ggatgttcgg ccatctgctg
    10681 atcatcggcg gcaaccatgg ctatggcggg gcggtgtcga tcgccgccga agcggcgctg
    10741 cgcgccgggg ccggactggt gacgattgcc acgcgcgggg cgcacctgag tgcactgctg
    10801 gcgcggcgac ccgaggcgat ggtgcgcgcg gtcgaatcgg gacaggatct ggagccgctg
    10861 ctggcgctgc cgaccgtgat cctgctgggg ccggggctgg gtcaggatgc ctgggccgag
    10921 cagatggtac agcgggtgct tggcagcgat ctgccgttgg tcgtcgatgc cgatgggctc
    10981 aacctgctgg cgcgccgcgc cacgcctgtg caccgcgaaa actggatcct gacaccccac
    11041 ccgggtgaag cagcgcgact gctcggctgc gacagccgcg aggtggaggc cgaccgcttt
    11101 gccgccgtgc agcagctggc cgaacgtcat ggtggtgtgg cgctgctgaa aggggccggc
    11161 accctggtgg ccagcgcagc ggtaggtggc gggattgcgc tctgtgccgc cggcaacccc
    11221 ggcatggcca gtggcggcat gggcgatctg ctgagcggac tcatcgccgc actgttggcc
    11281 cagcaattgt cgctggtcga tgcagcgcgg gccggggtgc tgctccatgc catggccggt
    11341 gacgaagccg ccggcggcaa cgagcgtggc ctgctggcca ccgatctgct ggcaccgctg
    11401 cgccggtggg tcaatccgct ctgacgcagg gtgcgcatgt ctgattcaac caccattctg
    11461 acgttgctgc tgcccgatga agtggcaacg ctgcgattgg gcggggtgct tgccgaggtg
    11521 gtcgctgcgg ggattctgtt cctgcgcggt gatctgggca gtggcaaaac gacgctgagc
    11581 cgcggcctgt tgcatggcct ggggcatcag ggcgcagtca aaagtccaac ctataccttg
    11641 gtcgaaccct atctgctgag cgaattggcg ctctatcatt tcgatctcta tcgttttgct
    11701 gatgaaaaag agcttgaata tcttggtgtt cgggattatt ttcgagaaga tagcttatgt
    11761 ctgatcgaat ggccggagcg gggtgagccc ctgttgccat cacccgatct tgaaatcagg
    11821 ctgaaatgga tcgaaaaggg gcgctggtgc gaaatcgagg gctgttcgga caggggacgg
    11881 atgtggttgg cgcagttgcg ctgcaatcct gtggtgcagg cattggccgt ggccgcagtc
    11941 gacgactcag cctgacaatc cgcttcggct cactgctgtg cctgctggtt tttttgctgg
    12001 cccagtcggc atcgggggca gccatcctca agagtgccaa ggtcgaaaac agcggagaga
    12061 gcagccgtct tctgctcgat ttttcggccg gcgtcaccca caagtttttc acgctgtcag
    12121 caccggatcg tctggtgatc gatctgaccg agacccagct tggcgctgcc ctcgatcggc
    12181 tcgatctggc ggcgacgccg atcgcccgca tccgcacctc gcaccagcca cagagcgcac
    12241 ggctggtgtt tgacctcaaa cagcgcacgc cggtgcgggt ctatctgact gcggccgagg
    12301 gcgcagcgac ccagcagcgg ttggtggttg agctgggcgc gcagaagaag acggcaaagc
    12361 cacccttgcc ggccgcggct gccacagcca ggtcagtgaa accgcctgtc gccgaaaaac
    12421 cgagatctcc gtccgtcacc cagccgccgc tcgtcaaggg gagtgagaag cccagccctg
    12481 cagcgtcgca gtcagcgcgg gtgaccgcag tggcgcccaa cgtcaaatcg gtgcaacagc
    12541 tcaaccacga acggcgagat ctggtgatcg cgatcgatgc cgggcatggc ggtgacgatc
    12601 caggcgcgct gggaccgggc gggatccgtg aaaaggagat cacgctggcc atcgccaggc
    12661 agttgcatgg cctgttgagt caggaacctg gtttcaagcc ggtgctgatc cgcagtggtg
    12721 accgtttcgt gccgctgcgt caacgcaccg agatcgcccg caagcacaag gccgatctgt
    12781 tcatttcaat tcatgccgac tccttttctg gcagcgatgc gcgtggcggt tcggtctaca
    12841 ccctgtcgca gcgcggggcc agcagcgaga gtgcgcgctg gctggccgat caggagaaca
    12901 aggccgatct ggtcggcggt gtcggactcg accagcgcga tggcatggtg gcgcaggtga
    12961 ttctcgacct ctacatgacc gccacgctca acaccagcaa tgaactggca tccgaactgt
    13021 tgcgcgggtt gaacaaagtt gggccgctgc atcggcagca agtcgaaaag gcggggttca
    13081 tggtgctgaa gtcgcccgat atcccgtcgg tgctggtcga aaccggcttc atctccaatc
    13141 ccagcgaggc gcgcctgctg aacacccggc agcatcaaca gcggctggca cagagcctgc
    13201 gtggcgccat tgtctcttac tacagccgcc gcccgcccga gggcacgatg ctggcctggc
    13261 gcaaacgggg cggcgatctc tccgccagcc cggcggtggc gcagaatgag tcgagcgagc
    13321 tgccagttcg acgctgagcc tctgtgacac gggctgcgtg tcgttctggc atcaaagtac
    13381 ccgcaaggcc agcagttcga tcagggaagg cagcagcacc agcagcagaa tcgccaccct
    13441 gcgccacagt gacttccagc cacgcgaacg gtgcagggtg atcatgatgc cgcccagcat
    13501 cgagcgactc aggttgcggc gatcgatcag actctggccc agcacgccga cgacatgcag
    13561 cagcacgagc agcagactga actgggccag cagttcatga ctgcctgcga gcagccattt
    13621 gtgccagacc atgcctgcgg ggaaaaaacc catcagccac cccttgccat ggtcgagggc
    13681 cagcagtgcc atgccggtga cgcagagcag cagcaggatg ctgaacaggg ccagaatgtt
    13741 gagcgcaccc agtggatgat ggccgtggtg cggcaagggg cgcagcgcca gcagggccgc
    13801 gagatgcgcc ttcacggccg tgggcgtgat ccagagttga tcgagggcgg ccggggtggt
    13861 gctgaagagt ccgcccagca gtcgcagccc gatcagcagc acgatcagat ggccggccag
    13921 cgcatgcagc cagagcgcct cctcgccgct gagataagcc agcacgaagc tggtggccag
    13981 cagccagtgg agcaaccgga tcggccaggg ccagagtcga tactgtcgtt ctgacatgcg
    14041 cgggtttcaa agcctgctgg cttgcccaga tggtgtggct gtcgtgtccg ggcgcaaacg
    14101 atgcgtggct tctgtcgatt cgagatgcag ggtggatgca ggcgtccctg cctgcatggc
    14161 cgactgttca ggagacgaat tgcggcgcgg cacccatgct ccagagaatc accgtggcgc
    14221 ccatcagtgc caccaccatc accaggccca ccgcgatcac cgcactggaa aagagtacgc
    14281 cgcgctcggg tgagatctcc atcatgatcg gcgtgccctg atagagcaga tagacggtgt
    14341 agccgattgc aacaagcccc accaccaggt cgaaccagag cgctggatag atgccgagta
    14401 gaccgctcag cagcaacggg gtgacggtgt atccggccag cttgaggctc cgggcaaagg
    14461 aggagttggc gccgtaggtt tgcgccatcc agtggatcag ggcacccagc agcatgacca
    14521 tcgccagaat ggcgccatag aagagcagcg cgatgatcag tgcactctgg ctggtgagtt
    14581 tggtggccgg accgctgccg atgctccagc caaaggtggt ggtgccgatg taccagcaca
    14641 gtggcggcag cagcgccagc cagatcaggt ggttgaacag atggtgcagc ggactctcgg
    14701 tttcgtcatg gatggattgc catgcctgat tgggacgggt gaagatctcg acggtatgcg
    14761 ccagcagcat tgggggtctc ctcctgaagt cgttattttc gattgcctgc ggtcttggcc
    14821 aagagtacaa aaccgctgat catgatgcca tagccattac aaagataatg ggcaaatgcg
    14881 cgctggcgtt cgttcacagc cagccactgc cgaccattcg cgcggaggca tggctccagt
    14941 gctgcaatag atagaccacg ctgatcatca ggctggcact gctccagctc cagccgggca
    15001 gcgtgccacg cagggccagc tcttcatgat ggcggtggca gaacagcgtc atcaggttgg
    15061 cgatcagcag accgaccagc aactccttga tcacagtggt aaagggtgga gcctcgaaat
    15121 agagctggct gatgccgaac aggctgatca gcgccgccat gccggtgtag atcacgaagc
    15181 ggtggggtgg cgtccaggat tgtgcgatca ggtaggcggc aaagccaagc ggtgcggtca
    15241 gggttgccag cggaagctgt cggttggcca attgcagcgt caacagcagc agtgcgaacc
    15301 agaaaccgag tgcatgccag ccgcgccgcg ccagcagcca gatggcgagc aggccagcct
    15361 gtccggccag gtgggggcgt tcagctagca gtttgagtgg cagcagcagc aacgtgatgg
    15421 ccggatggcg caacgaggag aaaaaatcgg ccacggcgcg gtcgtgaccg ttgagcagca
    15481 caccctggag ggcgaacagc atggccagca gcagcgccag cgccaggctg gcgagcggaa
    15541 aaacgccatc ggcgcgtccg ccgctggcgc gccagcgcca gagcagggca ccgatccttg
    15601 tcgatgtcag ttgtcgttgg cagaaacgat agaagagatg tcccggactc agtgccagat
    15661 agccaaagcg cagtccgtag agcgctacag agccgaccag cagcaggacg atgcccagtt
    15721 gcatggcatc ggcgatctgc gcctcgctct gctgcagtga gctgccgagc agatagccgg
    15781 gcagcagata gacgggtgcc cacagcagcg ccgagaacag gttggtgaga atgaagcgcc
    15841 ccaccggcat gtcgagcatc cccgccacca tcggaatgaa gggacggatt ggtccgacaa
    15901 aacggccgag cacgatgctt ttaccgccgt ggcggtaaaa gaatgcctcg ccattgtcga
    15961 tccattcggg atggcggcga aatggcccga actcacgaag atgctgatga aagtgatgac
    16021 cgatccagaa gctgatgcca tcgcccagaa tcgcaccgat catgcctgcc agcagcagcg
    16081 gcgcggcctc gaccgtgccg ctgccggcca gtacgctgat accgatcagc agcaccacgc
    16141 cgggcaccag caggccgacg atggccagcg actcggccag cgcaacgatg aagatggcgg
    16201 ccagggtcca gccggggtgg ctctgcagcc aggtcagcag ggtctcgatc aaggacatgg
    16261 actccggttc atcgatggtg cgacgcagaa ttgtagcgag tcggaccgct cgtcgacatc
    16321 gggatggcat tggaaccacc ctgaggcttt cattacaatg ggcgcttccc ccactgatga
    16381 tgtgcgtcaa atgaatcaac cgcttgcaca gcagatgcag cagattggcc tgcgcgcccg
    16441 cgaggcttcc cgcacgctgg cacgcgccag caccggcgac aagaaccgtg ccctcattgc
    16501 caccgccgag gcgctgcagc agcaccgcac caccttgctg catgccaacg gccatgacct
    16561 cgaagcagcc cgggcgcgcc agctcgaacc ggcgctgatc gagcggctga cgctcaacga
    16621 tgcccgcatc gatgcgatga tcgagagtct gcatcaggtg gcggcactgg ccgatccggt
    16681 cggcgccgtg accgacctgc ggcgcatgcc gagcggcatc gaggtggggc ggatgcgggt
    16741 gccgctgggg gtgatcggca tcatctacga gtcaaggccc agcgtgaccg ccgaggcggc
    16801 cagcctttgt ctgaagtcgg gcaatgccgc catcctgcgc ggcggttcgg agtcgcacca
    16861 ctccaaccgt gtcatcgccc aggcgatcca gcatggcctg gcgactgccc tgttgccgga
    16921 ccatgcgatt caggtgctgg agagcaccga ccgggcggcg gtgggccatc tgatcacgat
    16981 gccggagcat gtcgatgtga tcattccgcg cggtggccgc ggcctgatcg aacgcatcag
    17041 tcaggaagca cgcattccgg tgatcaagca cctcgatggc atctgccacc tctatgtcga
    17101 tgcgcatgcc gacctcgaga tggcactcaa gctggcggtc aatgccaaga cccaccgcta
    17161 tggcgtctgc aatgcgatgg agaccctgct ggtctgcgct gcggtggccg aacgctttct
    17221 gccgctgatc accgatgcgc tgaaggtgaa gggggtcgaa ctgcgtggtt gtacagagac
    17281 ccgccgttgg gtgccggact gcacgccggc ctgcgacgag gattggatca ccgagtacct
    17341 ggcgccgatc ctgtcgatcc gggtggtggc agggctcgac gaggcgatcg atcacatcaa
    17401 ccgccatggt tcgcaccaca ccgacggcat catcacccag cggctcgacc atgcccgtcg
    17461 cttcatcacc gaggtcgact ccagctcggt gatggtcaat gcctcgaccc gctttgccga
    17521 tggcttcgag tatggcctgg gggccgaaat cggcatctcc accgataagc tccatgcccg
    17581 tggaccggtc gggctcgagg ggttgaccag ccagaagtat gtggtgctgg gagacggcca
    17641 cattcgccaa tgagtggcct gctggcggaa cagccccgca accgcgccgt tggcttcatg
    17701 ggtggcacct tcgatccggt ccaccatggc cacctgcgct gcgcgctgga gatcaaggag
    17761 ctgctggcgt tggatgagat gcggctgatt ccttgtggcg atccctatca ccgctcggct
    17821 gagtcgctgc cggctgcagt gcgggttcag ctgctggaag cagcgctggt cgggaaccgg
    17881 gcgctcagga tcgacaggcg cgaactggca cgcaccggtc ccagctacag ccacgacacc
    17941 ttgctgtcgc tgcgcgggga actgggcgaa cagacagcgc tctgcatggt ggtcggcagc
    18001 gatgcctacc aggatctcga tcgctggcac cgctgggaga cactgctcga tctgggccat
    18061 ctgctggtgg tcgagcgtcc tggctggcaa ccgcaggagc ggccagcgct caaggcgctg
    18121 cgcggccgct gtgagaccga tcgcatcgat cggctgcgca acaggccggc cggcgcgatg
    18181 ctgtttctct cgctgcctcg gctcgagatc tcctccaccc gcatccgcgc cctgatcgcc
    18241 gccgggcgct ctcccgagta tctgctgccc gaagcggtct ggcagatgat tcgacagcag
    18301 cagctgtatg gttaccgatc caccgacacg gacaattctc ccgatgaacg ttgaaaccct
    18361 gaaagagcgt ctgctgacgg tgctggatga gcgcaaggca gagcagatcg tttgtctcga
    18421 tgtccacgcg ctcacctcga tcaccgactg gatgatcatc gcgaccggca cctcgtcccg
    18481 gcacaccaag gcggtggccg agtatctggt gcaggaggcc aagaacctcg gccatcaacc
    18541 caccggcgtc gaggggctgc aatcgggcga gtgggtgctg gtcgatctag atcggatcat
    18601 cgtccacatc atgctggccg aaacccgtcg tctttatgat ctggagcggc tgtggcgtca
    18661 gacaccgctc atgccagcgc aagattgagc atcgatgcgc attcgcctgc tggccgcggg
    18721 caatcgcccg ccggcctgga tcaagagcgg ctatgaagag tacatctccc gcctgcgccc
    18781 ctactgtcgc tttgagctga tcgagattcc gctgccatcg cgcggcaaat cattgtctgc
    18841 gcagcaggca cgccggatcg aaggcgaacg gatgctggcg gccctcggcg agcgtgaccg
    18901 gctgattgca ctggccatcg aaggtgaagc ctggagcact gcgcagctga gcgcacgcct
    18961 cggtgagtgg gagcgtgccg gcgaccctct ggcgctggcg atcggtggac cggatgggct
    19021 cgatgacgcg ctgctcgctc gcgcccagcg acgctggtca ctttctgcgc tgacgctgcc
    19081 gcacctgctg gtgcgggtga tcgtcaccga gcagctctat cgcgcccatg cgctgcgcga
    19141 caaccatcct taccaccgct gaaccgccat gtcccccagt ccggttttca aggaccatga
    19201 gcaggagcgc cggttggtgc agcgccgcct gctttggagc gtgctgctgc tgctgctgtg
    19261 catggccctg ctgctgggac gaatctacac cctgcaggtg gtaaagcatg aagagtttca
    19321 cacgctgtcg gacaagaacc gcattcgcct gcaaccgatt ccaccgaccc gcggcctgat
    19381 ctacgaccgc aatcagcagt tgctggcgca caacagggcg agtttcaacc tgacgctggt
    19441 caaggagtcg attgcagacc tgccgcagac gctcgatcgg ctcaccacct tgctgaaact
    19501 gaccgatgag gagctgaacc ggttcaggaa acggctcgaa cgcagccgct cgttcgatct
    19561 ggttccgctg cgcgacaacc tgaccgaaga ggagatcgcc attctggcgc tggagcggca
    19621 tcgcctgccg ggggtcgagg tgagtgcgca actggtgcgc cactatccgt ttgggccgct
    19681 gtttgcccat ccgctcggat atgtggcccg catcaacgag agtgagctga aggaggtcga
    19741 tccggtcaat tacagtggca ccgagtacat cggcaaggga ggggtcgagc ggcagaatga
    19801 agcattgctg catggtcagg tcggctatca gaatgtcgag atcgatgccc gcggccgggt
    19861 gatccgggtg ctgaacagca ccgcggcgca aaatggggct gatctcaccc tctacctcga
    19921 cagccacatt cagcaggtgg cggaagaggc gttgaccggt tggcgtggtg cggcggtcgc
    19981 catcgaaatc agcagtggtg gcgtgctggc aatggtgagc acgcccagct atgaccccaa
    20041 cctcttcgtg accggcatcg atgcccggcg ttatggcgaa ttgcagaaat ccaccgatct
    20101 gccgctgctc aaccgcgccg tacgcggacg ctatccgccc ggttcgacgg tcaagccgtt
    20161 cgtggcgctg gccggcttgc agcatcaggt gatcaatttc gattcgacgg tcgctgatcc
    20221 gggctggtat caattgccgg gcaacgagca caaataccgc gactggaaaa agtgggggca
    20281 tggctaccgg gtcggactgc gtcaggcgat cgtggagtcg tgcgacacct ttttttatga
    20341 tctggcttat cgactcaaca tcgatcggct ctccgacacg ttgggccgat tcggctttgg
    20401 cgcgctgtcg ggggccgaat ttcccggtga actgccgggg attctgccga gccgggcctg
    20461 gaaacaggcc agataccggg ccaactggtt tccaggcgag accgtgatca ccggcattgg
    20521 tcagggctac atgacggtga cgccactgca gcttgccgcc gccactgcca ccttggccaa
    20581 tcgcggcgtc atggttcgac cacgtctggt caagtcgatc tcgccagtcg acagtgcagc
    20641 gctgctgccc agcgcgccgc cgccgatcca gttggcgcag cgtggctact gggatttcgt
    20701 catcggcgcg atggaggggg tggtgcatga ccccactggc accgccaagc ggatcgcggt
    20761 ggacgcccct taccgtatcg ccggaaaatc gggcaccgcc caggtggtgg gcatcgcgca
    20821 ggacgaggag tatgacgccg gcaagatctc caaaaagaac tgggaccatg ctcttttcat
    20881 cgcctttgcg ccagccgaag cacctcggat cgccgtggcg gtggtggtcg agaatggcgg
    20941 cagcggcagc ggcacggcgg cacccatcgc ccgccgcatt ctcgatgcct atctgttgcc
    21001 gagacaacca tgagtcaatc tgatttcgtt cgc
//
LOCUS       DAORCQ010000035        20689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1492_length_20689_cov_5.999467, whole genome shotgun sequence.
ACCESSION   DAORCQ010000035 DAORCQ010000000
VERSION     DAORCQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 20689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20689
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1492_length_20689_cov_5.999467"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1286)
                     /locus_tag="PKI23_05455"
     CDS             complement(6..1286)
                     /locus_tag="PKI23_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN36419.1"
                     /translation="MHTATAVSDNRHGRLLFASLVGTTIEFFDFYIYATAAVLVFPTL
                     FFPGSDATSAMLQSFATFALAFFARPLGSALFGHYGDRVGRKATLVAALLTMGLSTLF
                     IGLLPTHAQIGLLAPLLLALCRFGQGLGLGGEWGGAILLATENAPAQRRAWYGMFPQL
                     GAPIGFILSGGSFLLLTHFLSDAQFFDWGWRIPFLASSLLVVVGLWVRLNLHETPAFQ
                     RAIDEQPPERLPIVTVLRDHPGLLLRGTLIALCTFVLFYLMTVFTLSWGTSQLHFSRE
                     RMLLIQLFGVLFFGLGIPISARLADRYDCRRVLIGASLGIALFGLLFSPLLDGGGEGA
                     MTLFMASGLALMGLTYGPLGTLLSGQFPTLVRYTGASLAFNLAGIFGASLAPYIATWL
                     ATHHGVHTVGLYLSGAALLTLAALWQAPESTAAD"
     gene            complement(1370..2419)
                     /gene="holA"
                     /locus_tag="PKI23_05460"
     CDS             complement(1370..2419)
                     /gene="holA"
                     /locus_tag="PKI23_05460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNN36420.1"
                     /translation="MKLRFEQLAGHLQGEMKPVYLVSGDEPLLLLEAADQIRQAARRR
                     GCDEREVHFAEPGFDWTRLLVSSQSLSLFASRRLLELRLTNKLPQAGAAVLEQCLAAP
                     SSDDTLLITAPRLDAEATRSRWHQAIDQQGVVVQIWPIEPRQMPQFLRQRLSRAGLEV
                     TADALELLAQRVEGNLLAAIQEISKLSLLHSGRGAVTVDDILDEVGDSARYELHLLAE
                     RALQGDTQAALRTLDGLLAEGVEPPLLLWSLHRELTTLALLHGRMAQGTATAQAIDQH
                     RPPIPQRRQPPLRQALQRLDRRWIERLLQQAALIDRAIKGAESIDIGSAFIDLVVGLS
                     APHHARLLLDTPLAH"
     gene            complement(2416..2943)
                     /locus_tag="PKI23_05465"
     CDS             complement(2416..2943)
                     /locus_tag="PKI23_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36421.1"
                     /translation="MNRRWLLIPLLMLGALLNGCGFQPRGTLPGQMQAGDLPYLDVVG
                     DRQNADLEFMLRRALIIQGFAREPGQAAPIRVKLLGTHQYEMPLSYDQRIQVAEYRLV
                     RSVSYRLIELQGKERSIPLQVSAERNYPAAPQNPSAYRQQLDEAVASINQELATRMAA
                     QLAKLCATEPASCRP"
     gene            complement(2940..5363)
                     /gene="leuS"
                     /locus_tag="PKI23_05470"
     CDS             complement(2940..5363)
                     /gene="leuS"
                     /locus_tag="PKI23_05470"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019098018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNN36422.1"
                     /translation="MQQQWEEARTFVVDERGDAPKFYCLSMFPYPSGRLHMGHVRNYT
                     IGDVISRYQRMRGHTVLQPMGWDAFGLPAENAALKNGVPPAKWTLENIDYMRGQLKRL
                     GFGYDWQREIATCHPDYYRWEQWFFIELFNKGLVYKRESLVNWDPVDQTVLANEQVID
                     GRGWRSGALVEQRSIPQWFLKITAYAQELLDALDSLDGWPEQVKTMQRNWIGRSEGVE
                     VDFALSDDAAAPLRVYTTRPDTLFGVTCLAVAPQHPLASAAAADNPALAAFIERCQNV
                     KVAEAELATLEKEGMPLGLTARHPLTGAEVPVWVANFVLMEYGSGAVMAVPAHDQRDH
                     EFASKYGLPIKQVISPVEAGTAIDLTQGAFTDKGILIDSAQFSGLGSAAAFDEIAKTL
                     QAQGRGHRRVNFRLRDWGVSRQRYWGCPIPIINCPQCGAVPVPAEQLPVRLPTEVVID
                     GVGSPLKRMPEFLHTTCPQCGGAAERESDTFDTFMESSWYYARFACRDGDSAMLDGRA
                     DHWTPVDQYIGGIEHAILHLLYARFFHKLMRDAGLLHSDEPFTRLLTQGMVLKDGTKM
                     SKSKGNTVDPQELIDHYGADTVRLFMMFAAPPEQSLEWSDSGVEGANRFLKRLWRLCH
                     ELPLDAAPVELATASLSESARAVRRKSHETIQRVSDDYGRRQQFNTAIAAVMELINLL
                     GKFDESSAAAIAVRREALDSALLLLSPIVPHITQALWQRLGHTGLIVDAGWPGVDERA
                     LQRDTVELVVQVNGKMRGKIEVARDADEATVKRLARDNDNVARFIAGQTELKTVVVPG
                     RLVNIVVKR"
     assembly_gap    5367..5466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5738..6520
                     /locus_tag="PKI23_05475"
     CDS             5738..6520
                     /locus_tag="PKI23_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNN36423.1"
                     /translation="MSESQLLVERRGAVQWITINRPEVRNALNDVVLNGIRDGIRDAM
                     QNPQLHAIVLTAAGDKAFCAGGDLKRGGGGPFSFSAAQIENPMIGLFKLVETCNLPII
                     ARVNGHVMAGGMGLLCACDLAVASDSALFGTPETKIGLFPMMILSYMLRIIPRRKLLE
                     MCITGEPIDAQEALRLGMINYAVPAAELDSKLDALLSTIVNRSPTAIKIGKHAFHAMQ
                     DMSIHEAFDYAQAVIPLMSQTDDAREGFASFNEKRPPDWPGN"
     gene            6553..7230
                     /locus_tag="PKI23_05480"
     CDS             6553..7230
                     /locus_tag="PKI23_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN36424.1"
                     /translation="MTGKLILEARQLSRSVTLPGATLTILDGVDLQVEQGQSLAIIGA
                     SGSGKSTLLGLLAGLDEPTQGTVWLDGVEITALDEEARARLRAQRVGFVFQSFQLLHG
                     LTALENVMLPAELNGLADPRQQALEWLTRVGLAGRINHYPRQLSGGEQQRVAIARAFA
                     SRPKLLFADEPTGNLDTVTAGAVIDLLFELNRSAGTTLILVTHDHALAARCDRRIELR
                     AGAVVNH"
     gene            7227..9728
                     /locus_tag="PKI23_05485"
     CDS             7227..9728
                     /locus_tag="PKI23_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN36425.1"
                     /translation="MSRSLGFAGRLLLRHLRGGELNVLLWALTLAVATVSSIALFADR
                     LQRAIVEESGAFLAADQVLTSPDPVPQAWLDQAERLGLRQARGVRFASMSFAGSAMQL
                     TAVAAYGAGYPLKGELKVSAEPFGAEEQVAQGPEPGTAWAEARLLALLGIGVGDQLEL
                     GAARLRITRVLVGEPDLGGDYFSVGPRLLMHLDDLAATQVVQPGSRVEYRYLFAAAPD
                     RLRDYGDWLRTRLTPVQRWIGLKEGRPPLATAVGRAEQFLLLGGSLGVLLCGAAVALA
                     ARRFSEDQLDAVAVLKSFGATSGQVLRIYLALLLYLGTLAVIIGELLGLLLQHLVIWS
                     LGDALPKQLPAAGWLPWSIGALTGFLCLAAFALPPLLRLRALSPLRVLRRDAISAPIS
                     TTLSHAAALLGMMALLRWYGGSWRLTLLLLLGLGVAAAVSALFSWLLLRGGRRLGMQA
                     GNLWRLALAGLQRRARSNYLQVLVFSVVLMLLLIIIVVRTALIDEWRRQLPEGAPNHF
                     ALNIAPQSVEPLRTFFASRQIAAAVIYPVARGRITAINGESPLAAAPLDQTQPENYGV
                     DREINLSWSTELPTGNRIIAGVWGQPVAGAEVTVSLEQEFASRHGLGLGDRLTLRIGE
                     TELLARVGTLRRVEWDSMQPNFYLLFQPDGMERFPHTYLTSFYLPPERKLLLNELLRA
                     FPSTSVIEVDALIRQIQRIVSQVTQAINLVLLLILLSGLLVLVASVRASMEERFLENG
                     LLRALGASRRLILGSLLAEFSLLGAAAGLLAVVGAELALWGLYREIFRIAPTWHPLLW
                     LLAPPLGALLVGGTGLLVSRRVVSTPPMQVLREYG"
     gene            complement(9762..10112)
                     /locus_tag="PKI23_05490"
     CDS             complement(9762..10112)
                     /locus_tag="PKI23_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36426.1"
                     /translation="MDLAQQSDAAIWRIVNPIMDNLMEASTAIDYARHVRDFTPRARS
                     MLNEEGFRSLCECYQAARGCFAEREPVALFRRPESVAAIWRQRFTRQPGDFVAEMVLV
                     QSGDRYLVDHVLVF"
     gene            complement(10212..11357)
                     /locus_tag="PKI23_05495"
     CDS             complement(10212..11357)
                     /locus_tag="PKI23_05495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36427.1"
                     /translation="MALVLNEEQNLLKTSAKDFFTNNMPVSQLRKLRDSKDAVGYSTE
                     SWQQIVELGWSGIIVEEEFGGLAFGYTGLGVVMEEAGRTLAASPLFATCVLGSSAITL
                     AGSKAQKSDLLPQIVQGSLTLALAIEESATHGPQRTRTTASKSGDGYTLNGEKVFVLD
                     GHSADMLVVVARTSGKPGDSSGLTLFLVDRNSAGVSVKRVFMADGRNAATVTFSNVAV
                     PASAVLGTVDQGSTALQDILSVGQILLAAEMLGSLQEAFERTINYIKTRVQFDRHIGS
                     LQAIQHRAAHMYAEIELAKSVVIASLAVLDEGVRGDELAKQASLAKAKLAEVFLLTSS
                     EGVQMHGGIGMTDDEEIGFFLKRARTAEHTLGDIRYHQNRFGDLLAI"
     gene            complement(11404..12591)
                     /locus_tag="PKI23_05500"
     CDS             complement(11404..12591)
                     /locus_tag="PKI23_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36428.1"
                     /translation="MSNLEQFRGEVREWLAANCPASLRQPGAVNDSQFTRKRRSGDSL
                     LWLERMAAKGWTAPTWPKQYGGGGLTPEENRVLQSEMRQINAPPALMGMSLTMIGPTL
                     LEMGTEDQKQRHIPKIVAGEVHWCQGYSEPNAGSDLANLQTKAEDKGDHFIINGSKIW
                     TSGAMYADWIFCLTRTDSTVQKQKGISFILFPMSNPGVTLRPIRLISGDSPFCQVFFD
                     NVRAEKCDLVGKLNEGWTVGKRLLQHERNSIGGGSTSRPGLEVGVLQVAKQYRGDENG
                     KVEDPLLRQQLIQHQINSQAFALTNRRIAEEAQAGQTPGFATSMLKYYGSELTKRGSE
                     LMVSMMGPRGLGQGDDTENFTENEREITRNWLFHKALTIAGGCSEVQLDIISKRILEL
                     PST"
     gene            12783..12858
                     /locus_tag="PKI23_05505"
     tRNA            12783..12858
                     /locus_tag="PKI23_05505"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12816..12818,aa:Asn,seq:gtt)
     gene            complement(13007..13990)
                     /locus_tag="PKI23_05510"
     CDS             complement(13007..13990)
                     /locus_tag="PKI23_05510"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNN36429.1"
                     /translation="MKPAVRVTVTGAAGQISYALLFRIASGDMLGKDQPVILQLLEIT
                     PALDALKGVVMELDDCAFPLLQSVVVTDDPLVAFRDSDCALLVGARPRGPGMERKDLL
                     AANAQIFSVQGQAIDQVASRAIKVLVVGNPANTNALICQRNAPNIDPRQFTAMTRLDH
                     NRALSLLGQKTQQPVTAIRRMTIWGNHSSTQYPDISQTTIAGSAAKGVIDNGWYQQEF
                     IPQVQQRGAAVIKARGSSSAASAANAALNHMRDWALGTPADDWVSMGIYSDGSYGIAK
                     GLIYSFPVRCAGGAFEIVQGLDIDAFSRERMTATEAELIEERDAVAHLLPG"
     gene            complement(14159..14671)
                     /locus_tag="PKI23_05515"
     CDS             complement(14159..14671)
                     /locus_tag="PKI23_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNN36430.1"
                     /translation="MKPALPLLLAAMLSWLAGCSTAPPRPAPPAAVAPSPAASSSGVG
                     PYQIFALAAAQGSLGIPYQFGGADPYRGFDCSGLVQYSYLQAGLLLPRTAAEQLAQTQ
                     PVTPERLLPGDLLFFRIDAPATNHVGLYLGSGTFLHAPSTGKRVRLDRLDDGYWRPRF
                     AGAGRVMPPP"
     gene            14783..15208
                     /locus_tag="PKI23_05520"
     CDS             14783..15208
                     /locus_tag="PKI23_05520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNN36431.1"
                     /translation="MPPFAIHPQLRADNHHLGRFHHCHLLLNRNAALPWFILLPEVAV
                     LEWHEIDEPLRQAIDREVQGLAAWVKRQFHCDKLNLAAIGNRVAQLHIHLIGRRQDDP
                     CWPDVVWGARLPPVEYGSEQIAELRRRLGAELGLMEVQP"
     gene            complement(15264..16037)
                     /locus_tag="PKI23_05525"
     CDS             complement(15264..16037)
                     /locus_tag="PKI23_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNN36432.1"
                     /translation="MARLLYVKAEVDSYTLCTDQETDMKRLALAAATAAALGAAPAFA
                     ATLYVPDFDSSGWQQYSYTFGGTFTGTVVLGVSDEGHDQISFLNALLPSQLLLDNLGG
                     TAVLPQLVSNRGFEFGNLAGYGAAGDVKVVSSFDSYSPTEGGQMALLDSVDGDSGVST
                     SLYLNTQGNPGVDGSLIQFSISANAGDTLTFDWAFSAGDYAPFGDFAFISLVGTGPAN
                     GHYEVLAEITPVPVPAAVWLMGSGLLGLAGVARRSQQKA"
     gene            16301..17785
                     /locus_tag="PKI23_05530"
     CDS             16301..17785
                     /locus_tag="PKI23_05530"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNN36433.1"
                     /translation="MGSVQQGVAARGDSPRFIIIGGGMAGILSAIKLREAGFTNFIGY
                     EKADRLGGTWRENRYPGVACDVPSHFYSYSFAPNPEWSHVFSPGEEIWSYFEEVARRY
                     DVVKDFRFGTEVRSMAFRDGRWHLELSTGERDVADFVIAATGVLHHPAYPAIAGLDRF
                     QGAMFHTARWDQSVPLDNRRVGIIGTGSTAVQIVSALVGRVAQLKLFQRTAQWIMPRP
                     NPPIAEADKALFRSDPAVMAAMRAELGKSASENFATAVVNINSPQLREIEQLCLANLE
                     ENVTDPVLREKLRPHYRAACKRLIMSPDFYQAIQRPNAQLVTESIEAVEAGGVRTVDG
                     VLHELDVLVLATGFQVDRFMRPIEVVGRNGMTLDEVWAKRPSAYLAISIPDFPNLFML
                     NGPNGPVGNFSLIDVAEIQFDYIMQLIEEARRRQAHQVSVKPQAAERFENERVEATRN
                     TVWVSGCRSWYLDDRGIPAAWPWGFDRFREVMAQPKFEDFELVA"
     gene            17968..19662
                     /locus_tag="PKI23_05535"
     CDS             17968..19662
                     /locus_tag="PKI23_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HNN36434.1"
                     /translation="MSPRNALLLFALIIPLTAQAWWNDAWSERRLVLLDTSEAGVATT
                     SAVSGAVLPVRLHSGNFDFLAAKPDGSDIRLLAADDKTPLKFHIERFDSVNELALLWV
                     QLPQIAPNSAGQKLYLYFGNPDAALEGSAPAASYDPSTRAVFHFGEGEATARDLSANG
                     NSASGELTIEKAGLLGASARFAQVPLRISASPSLQLAPDGRFSFSGWVRLDDVNGGAV
                     LYRQGPVAIRIEQGRLTLAGVGAQPLTGGALSAGSWQHIAVTVGGGKATLHLNGAEVA
                     AGEAALPAVQDEVLIGEGLRGLLDELQIASDLRSVDWIRLAVGAQGVESRLLAVRGDE
                     EVASEAGEVSYMGILVSNLTTDAWVVIVLLMVMLAIAVVVMVAKARLVSVTDKENRLF
                     LRRFRDATDDFLDLEKQGASHAHSSLYRLYQAAVREVMKRGSEQGGINLSNASISAIK
                     AAVDADLVRESHRLNAQMVLLTIAISGGPFLGLLGTVVGVMITFAAIAAAGDVNVNAI
                     APGIAAALLATVAGLAVAIPALFGYNYLASRIKNISADMQIFVDELITRIAESHSH"
     gene            19714..20124
                     /locus_tag="PKI23_05540"
     CDS             19714..20124
                     /locus_tag="PKI23_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNN36435.1"
                     /translation="MSVEIHEDNQPYDEINITPMLDLTYVLLVIFIIMTTASVQGVRV
                     EPPRTVTAQTLAKPQTRAITVTRDGNVFLDAYPVDMQQLEVRLAQYKVANPALPVVLK
                     GDAEAQYDKVMQVLEICKRLDIVEIGLVTKRAGG"
     gene            20127..20531
                     /locus_tag="PKI23_05545"
     CDS             20127..20531
                     /locus_tag="PKI23_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNN36436.1"
                     /translation="MQLQSETKPYDSMNVTPMLDLVYVLLVVFILMTTATVQGLTMNL
                     PKPSNKPSSEQHEVKIVQITPEGSLLLNGVGVTLEELKSQLEQAKARDPKLSVMIKGD
                     PLAQYARVIAVVDMANLLAIEGVGLVTARIGN"
BASE COUNT         3433 a         6300 c         7035 g         3821 t
ORIGIN      
        1 ggtggtcaat cggccgcggt cgactccggc gcctgccaca gcgcggccag cgtcagcagt
       61 gcggcgccgc tgaggtagag gccgacggtg tgcacgccat gatgggtggc gagccaggtg
      121 gcgatgtagg gcgccagcga ggcgccgaag atgccggcca ggttgaaggc gagcgaggcg
      181 ccggtgtagc gcaccagggt ggggaactgc ccgctcagca gggtgccgag cgggccgtag
      241 gtgaggccca tcagcgccag gccgctggcc atgaacagtg tcatcgcgcc ttcgccgccg
      301 ccatcgagca gcggcgagaa gagcagcccg aacagggcga tgccgaggct ggcgccgatc
      361 agcacccgcc ggcagtcgta ccggtcggcc aggcgggcgg agatcggaat gccgaggcca
      421 aagaagagca caccgaacag ctggatcagc agcatccgtt cgcggctgaa gtgcagctgg
      481 ctggtgcccc agctgagggt gaagacggtc atcaggtaaa aaagcacgaa ggtgcagagc
      541 gcgatcagcg tgccgcgcag cagcaggccg gggtggtcgc gcaggacggt gacgatcggc
      601 agccgttcgg gtggctgctc atcgatcgca cgctggaatg caggtgtctc gtgcagattc
      661 agccgcaccc agagtcccac caccaccagc aggctgctgg ccagaaaggg gatgcgccag
      721 ccccagtcga agaactgtgc atcgctcaga aagtgggtca gcagcagaaa ggagccgccg
      781 gagaggatga agccgatcgg cgcgcccagt tgcgggaaca tgccatacca ggcacggcgc
      841 tgcgctggcg cgttctcggt ggcgagcaga atcgcgccgc cccactcgcc gcccagcccc
      901 agaccctggc cgaagcggca cagcgccagc agcagcggtg ccagcaggcc gatctgggca
      961 tgggtgggca gcaggccgat gaagagggtg gagagcccca tggtcagcag ggcggccacc
     1021 agcgtggcct tgcgaccgac gcggtcgcca tagtggccaa acagcgccga accgagcgga
     1081 cgggcaaaga aggcgagcgc gaaggtggcg aaggattgca gcatcgcgct ggtggcgtcg
     1141 ctgccgggaa agaacagcgt gggaaagacc agcaccgccg cggtggcgta gatgtagaag
     1201 tcgaagaatt cgatggtggt gccgaccagg ctggcgaaca gcagccgtcc gtggcggttg
     1261 tcggacactg ccgtagcggt gtgcagcgcg ttgagctggg gtgaggcgtt ggccacggag
     1321 gggggttcct tgagcggtga cgagggggtg gatcgggggc gtgtcgcgct cagtgggcga
     1381 gcggggtgtc gagcagcagg cgggcatggt gtggtgcgct cagaccgacc accagatcga
     1441 tgaaggcgct gccgatgtcg atcgattcgg cacccttgat ggcgcggtcg atcagtgccg
     1501 cctgttgcag cagccgttcg atccagcggc ggtcgagccg ttgcagcgcc tggcgcagcg
     1561 gcggctgtcg gcgctgtgga atcggtgggc ggtgctggtc gatcgcctgg gccgtcgcgg
     1621 tgccctgtgc catccggcca tgcagcagcg ccagcgtggt cagttcgcgg tgcaggctcc
     1681 acagcagcag cggcggctcg acgccttcgg ccagcaggcc atcgagcgtg cgcagcgcgg
     1741 cctgggtgtc accctgcagg gcgcgttcgg ccagcaggtg cagttcatag cgggcgctgt
     1801 cgccgacctc gtcgagaatg tcatcgacgg tcactgcgcc ccggcccgag tgcagcaggc
     1861 tcagcttgct gatctcctga atcgccgcca gcaggttgcc ctcgacccgt tgtgccagca
     1921 gttcgagcgc gtcggcggtg acttcgagtc cggcccggct gaggcgctgg cgcaggaact
     1981 gcggcatctg ccgcggttcg atcggccaga tctgcaccac cacgccctgc tggtcgatgg
     2041 cctgatgcca gcggctcctg gtcgcctcgg cgtcgaggcg tggcgcggtg atcagcaggg
     2101 tgtcatcgct ggagggggca gccagacatt gctccagcac ggcggcgcca gcctgtggca
     2161 gcttgttggt caggcgcagc tccagcagcc ggcggctggc gaacagcgac aggctctgcg
     2221 agctgaccag cagccgcgtc cagtcgaagc ccggttcggc gaaatggacc tcgcgctcgt
     2281 cgcagccgcg ccggcgcgcg gcctggcgaa tctggtcggc ggcttcgagc agcagcagcg
     2341 gttcatcgcc gctgaccaga tagaccggct tcatctcgcc ctgcagatgg ccggcaagct
     2401 gctcgaagcg cagcttcatg gccggcaact ggccggttcg gtggcgcaga gtttcgccag
     2461 ttgcgccgcc atgcgggtgg ccagctcctg gttgatcgag gccacggcct catcgagctg
     2521 ctgccgatag gccgatggat tctgcggggc ggcggggtag ttgcgctcgg cgctcacctg
     2581 cagcgggatg ctgcgctcct tgccctgcag ttcgatcagc cggtaggaga ccgaccgcac
     2641 cagccggtac tcggcgacct ggatgcgctg gtcgtagctg agtggcatct cgtactgatg
     2701 ggtgccgagc agcttcaccc ggatcggggc ggcctggcca ggttcacggg caaagccctg
     2761 gatgatcagt gcgcggcgca gcatgaactc cagatcggca ttctgccggt caccgaccac
     2821 atcgaggtag ggcaggtcgc cggcctgcat ctgtccgggc agggtgccac ggggttggaa
     2881 gccgcagccg ttcagcaggg cgcccagcat gagcagcggg atgagcagcc agcggcggtt
     2941 catctcttca ccacgatgtt gaccagccgt cccggcacca ccaccgtctt gagttcggtc
     3001 tgaccggcga tgaagcgggc cacgttgtcg ttgtcgcggg cgagccgttt gaccgtcgcc
     3061 tcgtcggcat cgcgcgccac ctcgatcttc ccgcgcatct tgccgttgac ctgcaccacc
     3121 agttcgacgg tgtcgcgttg cagcgcgcgc tcatcgacgc cgggccagcc ggcatcgacg
     3181 atcaggccgg tgtggccgag ccgctgccag agtgcctggg tgatgtgcgg cacgatcggc
     3241 gacagcagca gcagtgcgct gtcgagcgcc tcgcggcgca ccgcgatggc ggcagcggag
     3301 ctttcatcga atttgccgag caggttgatc agctccatca ccgccgcgat ggcggtgttg
     3361 aactgctggc gccggccgta gtcgtcactg acccgctgga tggtctcgtg gctcttgcgc
     3421 cgcaccgccc gggccgactc gctgagagag gcggtggcca gttcgaccgg tgcagcatcg
     3481 agcggcagtt catggcagag ccgccacagc cgcttgagaa agcggttggc cccctcgacg
     3541 ccgctgtcgg accactccag cgactgctcc ggcggtgcgg cgaacatcat gaacagccgc
     3601 acggtgtcgg cgccgtaatg atcgatgagt tcctgcggat cgacggtgtt gcccttggac
     3661 ttggacatct tggtgccatc cttcagcacc atgccctggg tcagcagccg ggtgaagggt
     3721 tcgtcggagt ggagcagtcc ggcatcgcgc atcagcttgt gaaagaagcg cgcatagagc
     3781 aggtgcagga tcgcgtgttc gatgccgccg atgtactgat cgaccggcgt ccagtggtcg
     3841 gcgcgtccat cgagcatcgc gctgtcgcca tcgcggcagg caaagcgggc gtagtaccag
     3901 gaggactcca tgaaggtgtc gaaggtgtcg ctttcgcgct cggcggcacc accacactgt
     3961 gggcaggtgg tgtgcaggaa ttcgggcatc cgcttcagtg gtgagccgac gccgtcgatg
     4021 accacctcgg tcggcagccg caccggcaac tgctcggccg gcaccggcac ggcgccgcac
     4081 tgcgggcagt tgatgatcgg aatcgggcag ccccagtagc gttgccgcga cacgccccag
     4141 tcgcgcagcc ggaagttgac gcggcggtgg ccccgtccct gggcctgcag ggtcttggca
     4201 atctcatcga aggcggccgc cgagccgagg ccgctgaact gcgccgagtc gatcaatatt
     4261 cccttgtcgg tgaaggcgcc ctgggtcagg tcgatggctg tgccggcctc gacggggctg
     4321 atcacctgtt tgatcggcag gccatacttg ctggcgaatt cgtggtcgcg ctggtcgtgg
     4381 gccggcaccg ccatcaccgc gcccgagcca tactccatca acacgaagtt ggcgacccag
     4441 accggtactt cggccccggt cagcggatgg cgggcggtga ggccgagcgg catcccctct
     4501 ttctccagtg tcgccagttc ggcttcggca accttgacgt tctggcagcg ctcgatgaag
     4561 gccgccaggg ccgggttgtc cgctgcggcg gcgctggcca gcggatgctg cggcgccacc
     4621 gccagacagg tgacaccgaa cagcgtgtcg ggacgggtgg tgtaaacccg cagcggtgct
     4681 gccgcgtcgt cgctcagtgc gaagtcgacc tcgacccctt cggaacggcc gatccagttg
     4741 cgctgcatgg tcttgacctg ctccggccag ccatcgaggc tgtcgagtgc gtcgagcagc
     4801 tcctgcgcat aggcggtgat cttcaggaac cactgcggaa tgctgcgctg ttcgaccagc
     4861 gcgccggagc gccagccgcg gccatcgatc acctgttcat tggccagcac ggtctggtcg
     4921 accggatccc agttgaccag tgattcgcgc ttgtagacca gccccttgtt gaacagctcg
     4981 atgaagaacc actgctccca gcggtagtag tcggggtggc aggtggcgat ctcgcgctgc
     5041 cagtcgtagc cgaagccgag ccgcttgagc tggccacgca tgtagtcgat gttctccagc
     5101 gtccacttcg ccggcggcac gccattcttc agtgcggcat tctcggccgg caggccgaag
     5161 gcatcccagc ccatcggttg cagcacggtg tggccgcgca tccgctggta gcggctgatc
     5221 acatcgccga tggtatagtt gcggacgtgg cccatgtgca ggcgtccgct cggatagggg
     5281 aacatcgaca ggcagtagaa cttgggtgcg tcgccgcgtt cgtcgacgac gaaggtgcgg
     5341 gcttcttccc actgctgctg aatctgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnncgcc gcgttcgtcg acgacgaagg tgcgggcttc ttcccactgc tgctgaatct
     5521 gcggttcgag gcgttgcgga tggtagggtg tttctgtaga catgacgaaa ccggaaaatc
     5581 catcggcgcg ccgtgcgcgg cgaggcggca ggggcgcggc tggatgaggt tgaacgggtg
     5641 aaaatcaggc ccgctagcat aacgcagccg ccccccggca ggtaggggcc ggcagggtcg
     5701 gcatggaaag agcaattcaa tacaacagga ggcaacgatg tctgaatctc agctgctggt
     5761 cgagcggcgt ggtgcggtgc agtggatcac catcaaccgt cccgaggtgc gcaatgccct
     5821 caacgatgtg gtgctcaatg gcattcgcga cggcatccgc gatgccatgc agaatccgca
     5881 actgcatgcg atcgtcctga ccgccgccgg cgacaaggca ttctgtgccg gtggtgacct
     5941 gaagcgtggc ggcggtggtc ccttcagctt cagcgccgcc cagatcgaaa acccgatgat
     6001 cgggctgttc aagctggtcg agacctgcaa cctgccgatc atcgcccggg tcaacggcca
     6061 tgtgatggcc ggcggcatgg ggctgctctg cgcctgtgat ctggcagtgg ccagcgattc
     6121 ggcgctgttc ggtacgccgg agaccaagat cgggctcttt ccgatgatga tcctcagcta
     6181 catgctgcgc atcattccgc ggcgcaagct gctggagatg tgcatcaccg gcgagccgat
     6241 cgacgcgcag gaggcgttgc ggctcggcat gatcaactac gcggtgccgg ctgcggagct
     6301 cgacagcaag ctcgatgccc tgctgtcgac catcgtcaac cgctcgccga cggcaatcaa
     6361 aatcggcaag catgccttcc atgcgatgca ggacatgtcg atccacgaag ccttcgacta
     6421 tgcccaggcg gtgattccgc tgatgtcgca gaccgacgat gcccgcgaag gctttgcatc
     6481 gttcaacgaa aagcggccac ccgactggcc gggcaactga ccgatgccgt caacgcaacg
     6541 cacggaattc gaatgacagg caagctgatt ctcgaggccc ggcaactgag ccggagcgtc
     6601 acgctacccg gtgccacgct caccatcctg gatggggtgg accttcaggt ggagcagggc
     6661 caatcgctgg cgatcatcgg tgcctcgggg tcgggcaaat cgaccctgct cggtctgctg
     6721 gccggactcg atgagccgac ccaaggcacc gtctggctcg atggtgtcga gatcaccgcg
     6781 ctcgacgaag aggcgcgtgc ccggctgcgc gcccagcggg tcggcttcgt gttccaatcc
     6841 tttcaactgc tgcacggact caccgcgctg gagaatgtca tgctgccggc cgagctcaac
     6901 ggcctggccg atccgcggca gcaagcgctg gagtggctga cgcgggtcgg gctggccggg
     6961 cgcatcaacc actatccccg acagttgagc ggcggcgagc agcagcgggt ggcgatcgcc
     7021 cgcgccttcg ccagccggcc gaagctgctg tttgccgatg agccgaccgg caacctcgac
     7081 accgtcaccg ccggcgcggt catcgatctg ctgttcgagc tcaaccgcag tgctggtacc
     7141 acgctgattc tggtgaccca cgaccacgcg ctggcagcgc gctgtgaccg caggatcgag
     7201 ctgcgcgccg gcgccgtggt gaatcattga gccgttcgct gggctttgcc ggacggctgc
     7261 tgctgcgcca tctgcgcggt ggcgagctca acgtgctgct gtgggcgctg acgctggcgg
     7321 tggccacggt gagcagcatc gcgctctttg ccgaccgcct gcagcgcgcc attgtcgagg
     7381 agtccggtgc ctttctggct gccgatcagg tgctgaccag ccccgatccg gtgcctcagg
     7441 cgtggctgga tcaagccgaa cgactcggtc tgcggcaggc gcgcggggtg cgctttgcct
     7501 cgatgagttt tgccggcagc gccatgcagc tgaccgcagt ggcggcctat ggcgccggct
     7561 atccactgaa gggtgagctc aaggtcagcg cagagccctt tggtgccgag gagcaggtcg
     7621 cgcagggtcc ggagccgggc accgcctggg ccgaggcgcg gctgctggcg ctgctgggca
     7681 tcggggtcgg cgatcagctc gaactgggag cggcccggct gcgcatcacc cgggtgctgg
     7741 tcggtgagcc tgacctgggc ggcgactact tcagcgtcgg tccgcgcctg ctgatgcatc
     7801 tggatgatct ggcggcaacc caggtggtcc agcctggcag ccgggtcgag taccgctatc
     7861 tgttcgctgc ggcgcctgac cggctgcgtg actacggcga ctggctgcgc acgcggctga
     7921 cgccggtgca gcgctggatc ggtctcaagg aggggcgacc accgctggcg accgcagtgg
     7981 ggcgggccga gcagttcctg ctgctcggcg gctcgctggg ggtgctgctc tgcggcgccg
     8041 ccgtggcgct ggcggcgcga cgcttcagcg aggatcagct cgacgccgtg gcggtgctga
     8101 agagctttgg cgccacctcg ggccaggtgt tgcggatcta tctggcactg ttgctctacc
     8161 tcggcacctt ggccgtcatc attggcgaac tgctcggtct gctgttgcag catctggtga
     8221 tctggtcact cggcgatgcg ctgccgaagc agttgccggc ggccggctgg ctgccgtgga
     8281 gcatcggtgc gctgaccggt tttctctgtc tggcggcctt tgcactgccg ccactgctgc
     8341 ggctgcgggc gctgtcgccg ttgcgggtgc tgcggcgcga tgcgatcagc gcgccgatca
     8401 gcacgacgtt gagccatgct gccgcgctgc tcggcatgat ggcgttgctg cgctggtatg
     8461 gcggcagttg gcggctgacg ctgctgctgt tgctgggcct cggcgttgcg gccgcggtga
     8521 gcgcactgtt cagctggctg ctgctgcgcg gggggcgtcg cctcggcatg caggccggca
     8581 acctgtggcg gctggcgctg gcggggctgc aacggcgcgc gcgcagcaac tatctgcagg
     8641 ttctggtttt ctcggtggtg ctgatgctgc tgttgatcat catcgtcgtg cgcaccgcgc
     8701 tgatcgacga gtggcgtcgg caactgcccg aaggggcgcc gaaccacttc gccctcaaca
     8761 tcgcgccgca gagtgtcgag ccgctgcgca ccttctttgc cagccggcag atcgccgcgg
     8821 cagtcatcta tccggtggcg cgcggccgca tcacggccat caatggcgag tcgccgcttg
     8881 ccgctgcgcc actggatcag acgcagccgg agaactacgg cgtcgaccgg gagatcaacc
     8941 tcagttggag caccgagctg ccgacgggta accgcatcat tgccggagtc tgggggcagc
     9001 cggtcgccgg agccgaggtc acggtctcgc tggagcagga gttcgccagc cgccatggcc
     9061 tcgggcttgg cgatcggctc acgctgcgca tcggcgagac cgaactgctg gcgcgggtcg
     9121 gcacgctgcg ccgggtggag tgggacagca tgcagcccaa cttctacctg ctgtttcagc
     9181 ccgacggcat ggagcgcttt cctcacacct atctgaccag cttctatctg ccgcccgagc
     9241 gcaagctgct gctcaacgag ctgctgcgcg cctttcccag cacctcggtg atcgaggtcg
     9301 atgccctgat ccgccagatc cagcgcatcg tcagtcaggt gacccaggcg atcaacctgg
     9361 tgctgctgct gattctgctc tccggtctgc tggtgctggt cgccagcgtg cgcgccagca
     9421 tggaggagcg gtttctcgaa aatggcctgc tgcgcgccct cggcgccagc cgtcgactga
     9481 ttctcggcag tctgctggcg gagttctcgc tgctgggcgc tgcggccgga ctgctggcgg
     9541 tggtgggcgc cgaactggcg ttgtggggcc tctaccgcga gattttccgc attgcgccaa
     9601 cctggcatcc gctgctctgg ctgctggcgc caccactcgg cgccctgctg gtgggcggca
     9661 ccggcctgct ggtgagccgc cgggtggtgt cgactccgcc gatgcaggtg ctgcgcgagt
     9721 atggatagcg ctctgcgaaa acgtggcagt accaacccga gctaaaaaac cagcacatgg
     9781 tcgaccagat agcggtcgcc gctttgcacc agcaccattt cggcaacgaa atcgcccggc
     9841 tgccgggtga atctctgccg ccagatcgcc gccaccgact ccggccgccg gaacagggcc
     9901 accggctcgc gctcggcaaa acatcctcgg gcggcctggt aacattcgca gagagatctg
     9961 aacccctctt cgttcaacat gctgcgggcg cgtggggtga agtccctgac atgccgtgcg
    10021 tagtcgatgg cggtcgaggc ctccatcagg ttgtccatga tcggattgac gatacgccag
    10081 atcgccgcat cggattgctg ggcgagatcc atcgtgctac ctcgttgcac tgactgaaaa
    10141 tggggtggct ggacaaaaaa agcggaagcc gatctcccgg cttccgctcc tttgcagcct
    10201 gagcaggcgg atcagatcgc cagcagatcg ccgaagcggt tctggtggta acggatgtca
    10261 cccagcgtgt gctcggcggt tctggcgcgc ttgaggaaga agccgatctc ctcgtcatcg
    10321 gtcatgccga tgccgccatg catctgcacc ccttcgctgc tggtgagcag gaagacctcg
    10381 gccagcttgg ccttggccag actggcctgc ttggccagtt cgtcgccacg tacaccctca
    10441 tcgagcactg ccaggctggc gatcaccacc gatttggcca gctcgatctc ggcgtacatg
    10501 tgcgctgcgc ggtgctgaat cgcctgcagc gagccgatgt gccggtcgaa ctgcacgcgg
    10561 gtcttgatgt agttgatcgt gcgctcgaag gcctcctgca ggctgccgag catctcggcg
    10621 gcgagcagaa tctggccgac cgacaggatg tcctgcaatg cagtgctgcc ctggtcgacc
    10681 gtgccgagca cggcgctggc cggcactgcg acattgctga aggtgaccgt ggccgcgttg
    10741 cgcccatcgg ccatgaaaac gcgcttgacg ctgaccccgg cgctgttgcg gtcaaccaga
    10801 aacagcgtca gtccgctgct gtcgcccggt ttgcccgagg tgcgcgccac cacgaccagc
    10861 atgtcggcgc tgtggccgtc gagcacgaac accttctcgc cattgagcgt gtagccgtca
    10921 ccgcttttgc tggcggtggt gcgggtgcgc tgcggaccat gggtcgcgct ctcttcgatg
    10981 gccagcgcca gcgtcaggct gccctggacg atctgtggca gcagatcgct cttctgtgcc
    11041 ttgctgccgg ccagcgtgat ggcgctgctg cccagcacgc aggtggcgaa gagtggcgag
    11101 gcggccagcg tgcggccggc ctcttccatc accacaccga ggccggtgta gccaaaggcc
    11161 agaccgccga actcctcttc gacgatgatg ccgctccagc cgagctcgac gatctgttgc
    11221 cagctctcgg tggagtagcc gaccgcatcc ttgctgtcac gcagcttgcg caactggctg
    11281 accggcatgt tattggtaaa aaaatccttg gctgaggttt tcagcaggtt ctgttcttcg
    11341 ttcagtacta gggccatttt tataatctct gatgaaggtt gcgaattcgg gcgtgctgaa
    11401 ggctcaggtc gagggcagct cgagaatccg cttggagatg atgtcgagct gcacttcgga
    11461 gcagccgccg gcaatcgtca atgccttgtg gaacagccag ttgcgggtga tctcgcgctc
    11521 gttctcggtg aagttctcgg tgtcatcgcc ctggcccagg ccgcgcggtc ccatcatcga
    11581 caccatcagt tccgatccgc gcttggtcag ctcggagcca tagtatttca gcatcgaggt
    11641 ggcaaagccg ggggtctgtc cggcctgcgc ctcttcggcg atgcggcggt tggtcagcgc
    11701 aaaggcctgg ctgttgatct ggtgctggat cagttgctgg cgcagcagtg gatcctcgac
    11761 cttgccgttc tcatcaccgc ggtactgttt ggccacctgc agcacgccga cttcgaggcc
    11821 gggcctggag gtgctgccac caccgatcga gttgcgctcg tgttgcagca gccgcttgcc
    11881 gacggtccag ccttcgttga gcttgccgac caggtcgcac ttctcggcgc ggacattgtc
    11941 gaaaaagacc tggcagaacg gtgaatcgcc gctgatcagc cggatcggcc gcagggtcac
    12001 gccgggattg ctcatcggga acaggatgaa gctgatccct ttctgtttct gtacggtgct
    12061 gtcggtgcgg gtcaggcaga agatccagtc ggcatacatc gcgcccgagg tccagatctt
    12121 ggagccgttg ataatgaagt ggtcgccctt gtcctcggcc ttggtttgca ggttggccag
    12181 gtcggagccg gcgttcggtt cactgtagcc ctgacaccag tgcacttcac cggcgacgat
    12241 cttgggaatg tgacgctgtt tctgatcttc ggtgcccatc tccagcaggg tggggccgat
    12301 catcgtcagg ctcatgccca tcagggccgg gggcgcattg atctgtcgca tttcactttg
    12361 cagcacgcgg ttctcttcgg gggtcagacc gccgccacca tactgtttcg gccaggttgg
    12421 cgcagtccag cccttggcag ccatgcgctc gagccacagc aacgagtcgc cggagcgccg
    12481 ctttctggtg aactggctgt cgttgacggc accgggctga cgcaggctgg cggggcagtt
    12541 ggctgccagc cattcgcgca cttcgccccg gaattgttct agattgctca tgacgccctc
    12601 tcgttatgga tttattgaga cccgcaattc tatgcccaga gcggggcgct gcatagtttg
    12661 ccgcgcgcga cacaatggcg gaaagcacgg tgattgcggc gcaaggctga gccgtgccgg
    12721 acgatggcga agagattgac cccctgaatg tttttacgta gacttcgcgg ccttcgcact
    12781 cttccccgat agctcagcag gtagagcaga tgactgttaa tcatcgggtc gtaggttcga
    12841 gtcctactcg gggagccatc ttcttcgttc gcattccacg cacgctttga gccatccgca
    12901 ctcactgcgc ttttggcgtg gctgtgcttt gatcccgatg aaacgaaaag gccgccatca
    12961 ggcggccttt tgattcggga tgttgcccga tcaacctcgg tgcgcttcag ccgggcagca
    13021 gatgggccac ggcatcgcgc tcttcgatca gttcggcctc ggtggcggtc atcctttcgc
    13081 ggctgaaggc gtcgatgtcg agcccctgga cgatttcgaa cgcgccgccc gcgcagcgca
    13141 ccgggaacga gtagatcaat cctttggcga tgccatagct gccgtcgctg tagatgccca
    13201 tgctgaccca gtcatcggcg ggggtgccga gtgcccagtc acgcatgtgg ttgagtgcgg
    13261 cattggcggc cgaggcggcg ctcgatgagc cacgcgcctt gatgacggcg gcgccgcgct
    13321 gctgcacctg aggaatgaac tcctgctgat accagccgtt gtcgatcacg cctttggctg
    13381 cgctgccggc gatggtggtc tggctgatgt cggggtactg ggtcgatgag tggttgcccc
    13441 agatggtcat gcgtcgaatc gcggtgaccg gttgctgcgt cttctggccg agcagcgaga
    13501 gtgcgcggtt gtggtcgagg cgggtcatgg cggtgaactg gcgcggatcg atgttcggcg
    13561 cattgcgctg gcagatcagc gcattggtgt tggccgggtt gcccaccacc agcaccttga
    13621 tggcgcggct ggccacctga tcgatcgcct ggccctgcac cgagaagatc tgcgcattgg
    13681 ccgccagcag atccttgcgc tccatgccgg ggccgcgtgg ccgggcaccg accagcagcg
    13741 cgcagtcgct gtcacgaaag gccaccagcg gatcatcggt caccaccacc gactgcagca
    13801 gtggaaaggc gcagtcatcc agctccatga ccaccccctt gagcgcatcg agggcggggg
    13861 tgatctccag cagttgcagg atgacgggct ggtccttgcc cagcatgtcg cccgaggcga
    13921 tgcgaaacag cagggcatag ctgatctgtc ctgcggcgcc ggtgacggta acgcgcacgg
    13981 ctggtttcat cgaagattct ccttgatcaa gggtcggaat gaaaaagagg gcggtttgcc
    14041 agggtgggtc gggcagggtc tggagtgctc cgtcatgccc gcattcacgc gatgggaatt
    14101 ttaccatcct cgcgcgccat cgtccccggt tgctgccaga gtcggcgcag gaggggcgtc
    14161 aaggaggtgg catgacccgc ccggccccgg cgaagcgtgg tcgccagtag ccatcgtcga
    14221 ggcgatcgag ccgcacccgc ttgccggtcg aaggcgcatg caggaatgtg ccgctgccca
    14281 ggtagaggcc gacatggttg gtggcggggg catcgatcct gaagaagagc aggtcgccgg
    14341 gcagcaggcg ctcgggggtg accggctggg tctgcgccag ttgctctgcc gcagtgcgtg
    14401 gcagcagcag gccggcctgc agatagctgt actggaccag gccgctgcaa tcaaaccccc
    14461 ggtaggggtc ggcaccgcca aattgatagg gaatgccgag cgaaccctgc gcggcggcca
    14521 gggcaaagat ctgataggga ccgactccgc tgctggaggc ggcgggcgag ggggcgaccg
    14581 ccgcaggcgg ggccgggcgg ggcggggcag tgctgcagcc tgcaagccat gagagcattg
    14641 ccgccaacag cagtggcagg gccggtttca tcaggggttc gcctgcgtcg aacatgggtc
    14701 aagttcgttt tattttgaca tgtcattcat aaagatgaag cctgtttcat gattcattct
    14761 catctttttt tctggagccg tcatgccgcc ttttgccatt catccccagt tgcgtgccga
    14821 caaccaccat ctgggccgat ttcatcactg ccatctgctg ctcaaccgca atgccgcact
    14881 gccctggttc attctgctgc cggaggtggc ggtgctggag tggcatgaga tcgacgaacc
    14941 gctgagacag gcgatcgatc gtgaggtgca ggggctggcg gcctgggtga agcggcagtt
    15001 ccactgcgac aagctcaatc tggctgcgat cggcaaccgg gtggcgcaac tgcacatcca
    15061 tctgatcggc cgccgtcagg atgacccctg ctggcccgat gtggtctggg gtgcacgctt
    15121 gccgccggtc gagtacggtt ctgagcagat tgccgagttg cgccggcggt tgggtgcgga
    15181 gttgggactg atggaggtgc agccgtgagc agcgctgaaa tgcaaagggc cacccgcagg
    15241 tggcccttgc gtcaaccgtc tgttcaggct ttctgctgac tgcggcgggc gacaccggcc
    15301 agaccgagca gaccgctgcc catcagccag accgcggccg gtaccggtac gggtgtgatt
    15361 tcggccagca cctcatagtg gccattggcc gggccggtac ccaccaggga gatgaaggcg
    15421 aagtcgccaa atggcgcata atcaccggcg ctgaaggccc agtcgaaggt cagggtgtcg
    15481 ccggcattgg cggagatgct gaattggatg agcgaaccat caacgccggg attgccttgg
    15541 gtgttcaggt agagcgaggt gctgacaccg gagtcgccat ccacgctgtc gagcagcgcc
    15601 atctggccac cctcggtcgg gctgtagctg tcaaagctgc tgacgacctt gacgtcgcct
    15661 gctgcgccgt atcctgcaag attgccgaat tcgaagcctc ggtttgagac caattgcggt
    15721 agcacagcag tgccgccgag gttgtcgagc agcagttgcg agggaagtaa cgcattaaga
    15781 aagcttatct ggtcgtgacc ttcatcactg acgccaagaa ctaccgtgcc ggtaaatgta
    15841 ccgccgaagg tatagctgta ctgttgccag ccgctactgt caaaatcagg cacatagagg
    15901 gtggcggcga aggcgggtgc cgcgccgagg gcggccgcgg tggcggcggc aagagccagt
    15961 cgtttcatgt cggtctcctg atctgtgcag agtgtgtatg agtcaacctc agcctttaca
    16021 tacaacagac gtgccaataa ataattattt ttttttttat catatggtta tatcttcaaa
    16081 gttgatggcg aagagcattg cctggagcaa tctgtaaaaa aagttgacac ttttcggctg
    16141 gatggcgcgg ccggcagact ctgccgaagc cggcatttca ggcccatccg ccgggctgac
    16201 gagccattga cctgacacac tgtctggaca ttgccggcgc gctctgggag gatcatccct
    16261 cgacttggaa gtgtgaattt tttcaacagg gggagtgacc atgggcagtg ttcagcaggg
    16321 cgtggccgcg cggggcgatt cgccgcgctt catcatcatt ggcggtggca tggcgggcat
    16381 cctgagtgcc atcaagttgc gcgaggccgg ctttaccaac ttcatcggct atgagaaggc
    16441 cgaccggctg ggtggcacct ggcgcgagaa ccgctatccg ggggtggcct gcgacgtgcc
    16501 gtcgcacttc tacagctact cctttgcgcc caatccggag tggagccatg tcttctcgcc
    16561 gggcgaggag atctggagct acttcgaaga ggtggcgcgg cgttatgacg tggtgaagga
    16621 ttttcgcttc ggcaccgagg tgcgcagcat ggccttccgt gatggccgct ggcatctcga
    16681 gctctcgacc ggcgagcgtg atgtcgccga cttcgtgatc gctgccaccg gcgtgctgca
    16741 ccatccggcc tatccggcca ttgccggcct cgaccgcttc cagggtgcga tgttccacac
    16801 cgcccgctgg gaccagagcg tgccgctcga caaccggcgg gtcggcatca tcggcaccgg
    16861 ctcgacggcg gtgcagatcg tctcggcgct ggtgggtcgc gtcgcccagt tgaagctgtt
    16921 ccagcgcacg gcgcagtgga tcatgccaag gcccaatccg ccgattgcgg aagcggacaa
    16981 ggcgctgttt cgcagcgatc cggcggtgat ggcggcgatg cgggccgaac tgggcaagtc
    17041 ggcctccgag aatttcgcca cggcggtggt caacatcaac tcgccacaac tgcgtgagat
    17101 cgagcaactc tgcctggcca acctcgaaga gaacgtgacc gatccagtgc tgcgcgagaa
    17161 gttgcgcccc cactatcggg cggcctgcaa gcggctgatc atgtcgccgg acttctatca
    17221 ggcgatccag cggcccaacg cgcaactggt gaccgagtcg atcgaggcgg tcgaggccgg
    17281 cggggtgcgc acggtggatg gcgtgctgca tgaactggat gtactggtgc tggcgaccgg
    17341 ctttcaggtt gaccgcttca tgcggccgat cgaggtggtg gggcgcaacg gcatgacgct
    17401 cgacgaagtg tgggccaagc ggccctcggc ctacctggcg atctcgatcc ccgactttcc
    17461 caacctgttc atgctcaatg gtcccaacgg accggtcggc aacttctcgc tgatcgatgt
    17521 ggccgagatt cagttcgact acatcatgca gctgatcgaa gaggcgcgtc gccggcaggc
    17581 gcaccaggtg tcggtgaagc cgcaggcggc cgagcggttc gaaaacgagc gggtcgaggc
    17641 gaccaggaac accgtctggg tcagcggctg ccgcagctgg tatctggatg accgtggtat
    17701 tcccgccgcc tggccctggg gcttcgaccg tttccgcgag gtgatggcgc aaccgaagtt
    17761 cgaggatttc gaactggtgg cctgactgcc ggtccgggtg gtctggacag ccagatcacc
    17821 cgttcactgc cgccgtgcat cggcagatga gagatccttt gctcaagaag cgttctatct
    17881 ggcgctgccc ggcagggtcg gccgtcactt tttcgtcatc tgtcacggct agagtgccgg
    17941 tccgtcctca ctctgccgct gccgtccatg agtccaagaa acgcactgtt gctgtttgca
    18001 ctgatcattc ctctgaccgc gcaagcctgg tggaatgacg cctggagtga gcgtcggctg
    18061 gtgctgctgg acacatccga agcaggggtc gcgacgacct cggcagtctc tggcgccgtg
    18121 ctgccggtgc gactgcacag cggcaacttc gattttctgg ccgccaagcc cgatgggagc
    18181 gacattcgtc tgctggctgc cgatgacaag accccgctga aattccacat cgagcgcttc
    18241 gacagcgtca acgagcttgc cctgctctgg gtgcagctgc cgcagattgc gccgaacagc
    18301 gccgggcaga agctctacct ctactttggc aatccggatg cagcgttgga aggcagtgcg
    18361 cccgcggcca gttacgatcc atcgacccgt gccgttttcc atttcggtga aggcgaggcg
    18421 actgcgcgcg atctgtcggc caatggcaac agcgcaagcg gtgaattgac gatcgagaag
    18481 gcgggtctgc tcggtgcgag tgcacgattc gctcaggtgc cgctgcggat ctccgcatcg
    18541 cccagtctgc aactggcgcc cgatgggcgc ttcagttttt ccggctgggt tcggctcgat
    18601 gacgtgaatg gcggcgccgt gctctaccgg cagggtccgg tggcgatcag gatcgagcag
    18661 ggcaggctga cgctcgcagg cgtgggagcg caaccgctga cgggtggtgc gctgagcgcc
    18721 ggaagctggc agcacattgc ggtgacggtg ggtggtggca aggcgacgct ccatctcaat
    18781 ggtgccgagg tggccgcagg tgaggctgcc ctgccggccg tgcaggacga agtgctgatc
    18841 ggtgaggggc tgcgcggcct gttggatgag ctgcagatcg ccagtgatct gcgctcggtc
    18901 gactggatca ggctggcggt gggtgcccag ggggtggagt ccagactgct cgcggtgcgt
    18961 ggcgacgaag aggtggccag tgaagcgggc gaggtctcct acatgggcat cctcgtcagc
    19021 aacctcacca ccgatgcctg ggtggtgatc gtgctgctga tggtgatgct ggcgatcgcc
    19081 gtggtggtga tggtggccaa ggcccgcctg gtcagtgtca ccgacaagga gaaccgcctc
    19141 tttctcagac gttttcgcga tgcgaccgat gattttctcg acctggagaa gcaaggtgcc
    19201 agccatgcgc actcatcgct ctatcgtctc taccaggctg ccgttcgcga agtgatgaag
    19261 cgcggcagcg agcaaggtgg aatcaacctc agcaatgcct cgatcagcgc gatcaaggcg
    19321 gcggtggatg ccgatctggt gcgggagtcg caccgactca atgcgcagat ggtgctgctg
    19381 acgattgcga tttcgggcgg tcccttcctc ggtctgctgg ggacggtggt gggggtgatg
    19441 atcaccttcg cagccatcgc ggccgccgga gatgtcaatg tcaacgccat cgcgcccggc
    19501 attgccgccg cgctgctggc cacggtcgcg ggtctggcgg tcgccatccc ggcactgttt
    19561 ggctacaact atctggcctc gcggatcaag aacatctcgg cggacatgca gatcttcgtc
    19621 gatgagctga tcacccgcat tgccgagagt cacagccatt gagccgcctc tgacgcggct
    19681 gcatggtgcc atggtgcttg tcggggcctg acgatgtcgg tcgaaatcca cgaagacaat
    19741 caaccctatg acgagatcaa catcacgccg atgctcgatc tcacctatgt gctgctggtc
    19801 atcttcatca tcatgaccac cgcctcggta cagggcgtca gggtcgagcc tccccgcacg
    19861 gtgacggcgc agacgctggc caaaccgcag acccgtgcca tcaccgtgac gcgtgacggc
    19921 aatgtctttc tggatgccta tccggtggac atgcagcaac tggaggtgcg gctggcgcag
    19981 tacaaggtgg ccaatccggc gctgccggtg gtgctcaagg gcgatgccga ggcccagtac
    20041 gacaaggtga tgcaggtgct ggagatctgc aagcgtctgg acatcgtcga gatcgggctg
    20101 gtcaccaagc gggcgggagg ctgaccatgc agctccagag cgagaccaaa ccctatgact
    20161 cgatgaatgt cacgccgatg ctcgatctgg tctatgtgct gctggtggtg ttcatcctga
    20221 tgaccaccgc gacggtgcag gggctgacca tgaacctgcc caagcccagc aacaaaccga
    20281 gcagcgagca gcatgaggtc aagatcgtgc agatcacccc ggaagggtcg ctgctgctga
    20341 atggggtggg cgtcacgctg gaggagctga agagccagct cgagcaggcc aaggcgcgcg
    20401 atcccaagct gtcggtcatg atcaagggcg atccactggc gcagtatgca cgggtgatcg
    20461 cggtggtgga catggcgaac ctgctggcca tcgagggggt tgggctggtg accgcgcgca
    20521 tcggcaactg acatgagcgg actggccgaa gaggagctgg agcgcagtga tgccagtcgc
    20581 cgctggcgca tcctggccgg gttgctgctg ctggcagcgg gcattgcggt gaccctctac
    20641 ctgttgcagg ggctgggcag gagtggcccg gtgcagcaga agcaggtga
//
LOCUS       DAORCQ010000036        20575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1517_length_20575_cov_5.658869, whole genome shotgun sequence.
ACCESSION   DAORCQ010000036 DAORCQ010000000
VERSION     DAORCQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 20575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20575
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_20575_cov_5.658869"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKI23_05550"
     CDS             <1..787
                     /locus_tag="PKI23_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNN36437.1"
                     /translation="LDRAFGWAGELGLQVVIDLHAAPGCQNGFDNGGIKDVCEWHTEL
                     AYRDHSLAVLERLAERYGAHPALHAIEVLNEPRWDVPTDYLKAYNVDAYRRIRRHCPA
                     DRVAVIFHDGFRPFQEYCNFMQPPEFANVRFDIHRYQCFERGDIDMDIHGHIGKAAGA
                     WRDEADAIQRELRLPAYVGEWSLGLDLKVVSLWAQGPFNHALERMDALQETVAYRAYA
                     AAQLMTFERYAGWFFWSYRTETTPAWCFRDCVERGWLPDDFNP"
     gene            complement(777..2588)
                     /locus_tag="PKI23_05555"
     CDS             complement(777..2588)
                     /locus_tag="PKI23_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNN36438.1"
                     /translation="MPPLDSSPNPVSPAIGPIRSRYLFLHRFIRLAGPYWSSEQKWKV
                     RAITLALLLLTLAQVALVIWTNYWSRALFDALEARSLALFLRHGFTFVLIFVLTMLVT
                     ALHLQVKRWLQIGWRHWLTERLLGQWMVQGHHYQLQFTAGEHDNPDSRIAEDIRIATE
                     MAIMLAHTLVYSLLILGSFVDILLSVSGSTSLPGTSLQVPGYMVILALLYAGAGTALG
                     LLLGRPLVKATNRLQTLEADFRFGLSRSRENSEAIALMQGEQNERQHSLRQFDALGQG
                     WNRQTLAYMGIVSFSSGYGALLPVFPILVAAPQYIAGVMTLGVLMQSAQAFQKLTSAL
                     SWPIDNLGDMAKFRASAERMLSLHDDLSALAAESRQGDGRHIKRQKSARHELQLRDLS
                     IANPDGKLIIEHLDERFRRGERVWIDGDATAAISLFKAIAGLWPWGEGEVLLPRGQPI
                     YFMPQRPFLPDGTLRSLLSYPAPADRFETGSIHYALECVGLSWLAARLDESDQWDHVL
                     ALRTQQRLGFARLLLHRPAWIFIEEASDALAPKAEDCLMEMLHRELPNATLLTIGFHE
                     TLARHHDRKIMLTRVCGEKYLFHNPQVCWLEERPLKG"
     gene            complement(2664..3059)
                     /locus_tag="PKI23_05560"
     CDS             complement(2664..3059)
                     /locus_tag="PKI23_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HNN36439.1"
                     /translation="MQSRATHSKTIGYLLWIFGFIGAHRFYFGKPVTGTLWFFTLGLL
                     GIGWLIDLLLIPAMAREAEEHYQTGAVDYSTAWILLTFLGLFGVHRFYLGKWPTGLLY
                     LCTFALGGVGYLYDYWTLNSQIDALNRHA"
     gene            3305..3646
                     /locus_tag="PKI23_05565"
     CDS             3305..3646
                     /locus_tag="PKI23_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36440.1"
                     /translation="MKFVNGVLVCGLLSLSMSALAGSTTDAAIGGAVGGAVGAAVGNE
                     VGGREGAIVGGAIGAATGTAITTKGDEKHDGHHDHDDDHYRRVERDHDDDHHHHGSSF
                     CPPGQAKKGRC"
     gene            complement(3662..7387)
                     /gene="metH"
                     /locus_tag="PKI23_05570"
     CDS             complement(3662..7387)
                     /gene="metH"
                     /locus_tag="PKI23_05570"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNN36441.1"
                     /translation="MTSRAARIEQLNTALKQRILVLDGAMGTMIQGYRLDEAAYRGER
                     FRDHPHELKGNNDLLTLTQPEVIREIHTAYLEAGADIIETNTFNSTRVSQADYGLQAL
                     AAELNLAGARLAREVADAVTARDPSRPRFVAGILGPTSQTTSISPDVNNPGYRSISFD
                     QLVEEYREATAALVEGGVDLILIETVFDTLNCKAAIFAVRRYFDEQQFALPIMISGTI
                     TDASGRTLSGQTPAAFWHSVAHAEPIAIGFNCALGASELRPHIAELARIADVPVSAHP
                     NAGLPNEFGEYDETPEQMMAVVEEFMQSGLLNIVGGCCGTTPDHIRRLAEAAARNRPR
                     PIPQLDPACRLSGLEPLVIDASSLFVNVGERTNVTGSARFARLIREENYGEALEVARQ
                     QVESGAQIIDINMDEGMLDSRAAMVTMLNLIASEPDISRVPIMIDSSKWEVIEAGLKC
                     VQGKSIVNSISLKEGEASFLHQARLCRRYGAAVVVMAFDEAGQADTLARKQEIMQRSY
                     RLLVDEVGFPPEEIILDPNVFAVATGIEEHNNYAVDFIEACAYVKTHLPHALTSGGIS
                     NVSFSFRGNNPVREAIHAVFLYHAIRAGLTMGIVNAGQLAIYEEIPAELRERVEDVIL
                     NRRADGTERLLEIAEKYLHEGGSGSRPEAEQLAWRELPPAQRIIHALVKGISTFIVED
                     TEAARQEVERPIELIEGPLMEGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLTPYI
                     EAESAGGNIEKKGRILLATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVSCERILHA
                     AREQQVDVIGLSGLITPSLDEMVHVAKEMQRQGFKLPLLIGGATTSKAHNAVKIEPCY
                     QNDLAVYVPDASRSVGVVTSLLSDTLRDDFVAQRREEYATIRERSGRRNQAARLIPYA
                     EACQRGFVTDWRRYRPPVPNELGIRTLRNYPLEKLIDTIDWTPFFMTWELSGKYPNIL
                     DDAVVGENARELLADAEEMLEGIMVNGLLSANGMFGLFPANRVGSDDVVLFTDERRQE
                     RLATFHFIRQQGERPEDAPCLSLADFIAPLDSGVADYLGLFVVTGGLGAEELAAKYEA
                     VGDDYSAIMVKALADRLAESFAEHLHLRIRREFWGYAPDEQLDNPALIHERYRGIRPA
                     PGYPACPDHSEKATLFRLLDAAQSIQVQLTEHFAMTPAAAVSGFYFSHPDSRYFGVGK
                     IGRDQIEALAQRKGMPISELERWLSPNLAYDPDSNQA"
     gene            7525..8109
                     /gene="nfuA"
                     /locus_tag="PKI23_05575"
     CDS             7525..8109
                     /gene="nfuA"
                     /locus_tag="PKI23_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017136914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HNN36442.1"
                     /translation="MTPSLTITDAAQEYIAELLAKQGGEVNIRVFVADPGTPKAESCV
                     AYCRPGEEEADDLPLELRGFKAFIEARSLPFLEEALIDYAKDRFGGQLTIKAPHAKIG
                     RIDASSPLEDRINHVLYVEVNPNLAAHGGEVSLVEVVDGSIAVLKFGGGCQGCGMVDA
                     TLKEGVERTLVTQIPEITAVRDVTDHSVRDHAYY"
     gene            complement(8183..9622)
                     /locus_tag="PKI23_05580"
     CDS             complement(8183..9622)
                     /locus_tag="PKI23_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNN36443.1"
                     /translation="MRNRLLMPLLLLSTLPGCLLGPDHLQPQSDLPSGWMVPAADSAA
                     ATVTPTIDPHWWHLFDDPALTALIEEALQHNADLSVAAARIAEARALLNLRDAERYPL
                     LSGQADASRQQGSAETAQPGGGVIHDALSVSAVLNYELDLWGRLARANEAAQAQLLAS
                     VASRDAIALAVSADIASGYFNLRALDQQLLVARNTVKTREGSYRFQESRYRNGATSQL
                     VFRQAESELAAARAELPALEQQRTLQINALSVLLGRTPRELVQQTITTGHTLDELPPL
                     PEVPMGLPSTLLQRRPDILAAEQQLRAANAEIGIAHAAYFPRLSLTGLFGNQSDALSN
                     LFNGSAKNWQFAGNLTGPIIDFGRTQATVEAAEARRQQALINYQQTVRTAFREVLDAL
                     KSRSASSERLAAQSQQVAALQQTARLAQRRFEEGYSDYLEVLDAQRSLFNVELARINT
                     QQQGLRALVQLYKALGGGWQPDATPPHSG"
     gene            complement(9615..12755)
                     /locus_tag="PKI23_05585"
     CDS             complement(9615..12755)
                     /locus_tag="PKI23_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNN36444.1"
                     /translation="MISAFFIRRPVFASVLSIVIVLAGLVSMRALPVAQYPDIVPPEV
                     VVITSYPGASADVIAKTVAAPLEQEINGVEDMIYLRSVAAASGQLRITVSFAIGTDPD
                     QATINVNNRVTAAQARLPEEVRRQGIRVQKRSTSILGLITVSAPGGQYDSMFLSNYAL
                     LNIIDELKRIPGVGDASLFGQKDYSMRIWLRPDKLAQFNLTASDVAQAIQEQNSQFAA
                     GQFGQEPISADNAFTYTVNTQGRLADPAAFGQIILRSDDSGATLRLSDVARVELGAQD
                     YNFDASYKGRTTVPIGLYLQPGANALQTMDAIRASMVEMSQRFPEGLAYEIPFDTTRF
                     VRVSIEEVVHTFIEAIVLVVLVVFLFLQNPRATLIPLLAVPVSIIGTFAGMLLLGFSI
                     NLLTLFGLVLAIGIVVDDAIIVIENVERLMSQEGLSPREAAFKAMAEVTGPVIAIVLV
                     LSAVFIPVAFLGGLSGQMYKQFAITIVVSVVISGIVALTLTPALCALLLKPVHSEPAL
                     PFRLFNRMFARITDGYVNASGFLMRRTVLGMTLFTGLMAIALLLFNRLPGGLVPAEDQ
                     GYVLMAYQLPAAAALDRTRAITDVMTQRLMEQKAVESVVTFAGFDILAQSQRSNAGIS
                     FVMLDPWDQRTTSEDDARIMVNPLAHLGADLRDGEMFGFNPPPITGISTTGGFEAYLQ
                     DRTGSGGTALVETAHRLLDSASKRPELTELRTTFSTSTPQYYAELDREKARALKVPIN
                     QVFTIMQSTFGSLYVNDFTLFGRSYRVNLQSEAQFRRSPEDLRHVFVRSSDGNLVPLD
                     ALVKVNRTIGPDLVERFNVFPAAKILGQPAPGYSSGQAIAAMQAVSQEVLGANYSLGW
                     VGSAYQELASAGSGAQSFVFGILMVFLILAAQYERWSLPFAVVTAVPFAVFGAILATW
                     LRGLENDLYFQIGLLTLIGLSAKNAILIVEFAMLHRREGVSLMEAALQGARQRFRPIV
                     MTSLAFMLGVLPLAISSGAGSASRHAIGTGVIGGMIAATFLATLFVPMFFLLIGRLTE
                     RDGVTHTREKESDPHA"
     gene            complement(12752..13960)
                     /locus_tag="PKI23_05590"
     CDS             complement(12752..13960)
                     /locus_tag="PKI23_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNN36445.1"
                     /translation="MIRPATRLPTRHLPTALLCTLLLTTLTACDRKQDGNSHAQPTPE
                     VGIMVVQPRDVPIENEYAGRTAGFREVEVRARITGILQKRTYVEGQQVKAGDLLFRID
                     PAPFEVALTRAQARLSDAEAQLRQAERDWTRVAALFAENAISARERDSVQSALDLARA
                     NLAVANSDVEAARIDLGYTKVVAPVSGITSLEVRSEGSLVGPGSADSLLTRITQLDPL
                     YVNFSYPDTDALRQRQLLASGAMTMTGGERLAAELRFSDGTVYDQQGYISFTDSSIDP
                     ATGTVRARALFANPDNSILPGQFVRIVPKGLVRKGVMVLPQAAVMQGPQGAYVYLLAE
                     GDRARVQPIQPGITIGSQWLIEEGLKPGDKVIVDGVIKVKAGEPVKPAEPRPTPAPVA
                     NQASQSGTRP"
     gene            14166..14774
                     /locus_tag="PKI23_05595"
     CDS             14166..14774
                     /locus_tag="PKI23_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNN36446.1"
                     /translation="MARRTKMEAEQTREQIIDAAEELFLTRGVARATLEQIAQAAGVT
                     RGAIYWHFRNKTDLFDAMYCRVHDPVEDLVSQSLALEDEGAIEALRNFCVQSLLSLAR
                     DRRRRRVYSILFHHCEFVDGLQESNQRLIAIQHRGLASMEALFRRAREQGSLPNVCPR
                     LAAIVIHTSMLGLYFDYLREPDAYDLEREAAPLVDLIFRCIT"
     gene            complement(14843..16066)
                     /locus_tag="PKI23_05600"
     CDS             complement(14843..16066)
                     /locus_tag="PKI23_05600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNN36447.1"
                     /translation="MPSPEIFATLDIRTLILVTALIYLMCTLTQGFIYLNNRDEPGLG
                     KFVIAHALINLGLILIAARGLIPDLLSMVIANASILSGLTFLHHAIRCFYQRPTSLRA
                     TAVVFVLSCLAFFHFGQISPDYAIRVLLFSLPFIVLCILIAREFFLPTEHEIQPAPRW
                     VNATLFTLLALLFTVRLLVIMDAHLLASSIIRIGTESSLLQASPLSSLTWLVLIASFG
                     YWNVGLLLLVSQKFLARQNRLAITDGLTGILNRRGFELMAEKILLTRTASSNFMLLLD
                     LDHFKQVNDRHGHEAGDEVLRQFVLLLNQQLRKSDLTGRIGGEEFAVLLRESTEAQAW
                     SVAERVRTALQQMPIRHRDTTIEVTVSIGVAQSGADGESFAELFRTADQRLYQAKRSG
                     RNRTLFRETADVATP"
     gene            complement(16175..17428)
                     /locus_tag="PKI23_05605"
     CDS             complement(16175..17428)
                     /locus_tag="PKI23_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36448.1"
                     /translation="MKAVRQAAERMLRLLVALLALLLAAGLWLATAPAPLAMPDRPRP
                     TADIAPFPQRIKQSATRSNQALTLQASELDLFFRAALGHLPGTRQATSQVRIEPGTLH
                     LLASTPLSFWPARHLNLICRVTATPLEILTTRCRLGALPLPALSLAQLNGWLREGTKL
                     WPQIDFLQQTLNGIDRIEIGADRLTLHHHIDRQQVRSIVAEGGRWLWQGIDHAAVAPY
                     NERLRQLISQTDDALSLATALPALFRLAMERSGAAGSAQGENRAAITALALYAAGMAE
                     PFVRDRDHPVALTLHRRSDLAQHYLLSALITLKSLPQTAEWIGLQKELNDRQESGNDF
                     SYLDFAADLAGIRLATLATDDETGARQLQQVVSALPSDDGTLMPALKTLPKEGTHVGF
                     DTTRHQQIESEIRMAINRLAAYRLP"
     gene            complement(17403..19169)
                     /gene="ggt"
                     /locus_tag="PKI23_05610"
     CDS             complement(17403..19169)
                     /gene="ggt"
                     /locus_tag="PKI23_05610"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNN36449.1"
                     /translation="MSLSPVVARQTIIARLLLCGLILLGGAGRSWAVDENRAPEAATG
                     SRSKSAVTASRYLVVAADPQAAEAGRRILAAGGSAVDAAIAVQMVLNLVEPQSSGIGG
                     GAFMLHWTHRTGQLISYDGRETAPAAATPDLFLNNDGAPMKMLQAIVGGRSVGTPGVL
                     AMLELAHQRHGRLPWRQLFTDAIRLADRGFIVSPRLAELLQKNSNPGLLEPGAAHDYF
                     YPAGQALTAGSRLTNPAYAKLLRRIAIEGPTAFYRGKTAQRMVDAVQAHHIPGPLRLT
                     DLAHYQPKERPPLCGTYRGHRICSMGPPSSGGVTLLQMLALLEPFDLAAFRPVSVEAA
                     HLFAQAGRLAFADRALYLADSDFVPVPLKALLDPDYLRQRARLINPQHDMGVAAAGQL
                     SLLTQAEDRSPELPSTSHFSIIDRDGNAVSMTSSIETAFGSTLMVDGFLLNNQMTDFS
                     FLPEQNGQPVANRIAGGKRPRSSMTPAMVFDEQGELRWILGSPGGARIIPFVAQSLIA
                     LIDWRLDPQRTVDLPHYQNRNDRTELEAGTAAVTLREPLEALGHPVDVRDLSSGIHLI
                     ERNPTGLIGAADSRREGSAAGR"
     gene            19168..19830
                     /locus_tag="PKI23_05615"
     CDS             19168..19830
                     /locus_tag="PKI23_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016702194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNN36450.1"
                     /translation="MIDLYTAATPNGYKASIMLEEIGLDYNVIALNLAAGEQKSPAFL
                     AINPNGRIPAIVDRDEEDFAIFESGAILVYLAEKSGQLLPTDRKGRSRVMQWLMFQMG
                     GIGPMQGQANVFFRYFEEKLPAVISRYQNETRRLYQVLDGQLAQHEYLCGDYSIADIA
                     NWSWVRAHNWAGVAIDDLPHLQRWHDTLQARPACVRGIAVPPRPPRSDATIAGARQML
                     TR"
     gene            complement(19837..20445)
                     /locus_tag="PKI23_05620"
     CDS             complement(19837..20445)
                     /locus_tag="PKI23_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNN36451.1"
                     /translation="MTSPPAARSIVIDDGELTLWPDAFVLSWPDEPSLFEQLNSAIPW
                     RQDEITLFGKRHRLPRLQAWYGDPGASYRYSGLLLQPTPWQPLLNEIRRQVETLTGLR
                     FNSALANLYRDGNDAMGWHSDDEAELGVDPAIASVSFGASRRFLLRHRRRKDPPRIEI
                     LLTDGSLLLMAGPLQTHWHHALPRSRGVSEPRINLTFRLIRP"
BASE COUNT         3811 a         6148 c         6810 g         3806 t
ORIGIN      
        1 gctcgaccgc gccttcggtt gggcgggcga gctggggttg caggtggtca tcgacctgca
       61 tgccgcacct ggctgtcaga atggtttcga caatggcggc atcaaggatg tctgcgagtg
      121 gcacaccgag ctggcctacc gcgaccactc ccttgcggtg ctggagcggc tggccgagcg
      181 ctatggtgcc catccggcgc tgcatgcgat cgaagtattg aacgaaccgc gctgggatgt
      241 gccaaccgac tatctgaagg cctacaacgt cgatgcctat cgacgcattc gtcgacactg
      301 ccctgctgat cgggtggcgg tgatctttca tgacggcttc cgtccgtttc aggagtactg
      361 caacttcatg cagccgcctg agttcgccaa tgtgcgcttc gacatccacc gctatcaatg
      421 tttcgagcgt ggtgacatcg acatggacat ccatggccac atcggcaagg cggccggtgc
      481 ctggcgcgac gaggccgatg cgatccagcg cgaactgcga ctgcctgcct atgtcggtga
      541 atggagtctc ggcctcgacc tgaaggtggt ctcgctgtgg gcgcagggac cgttcaacca
      601 tgcgctggag cggatggacg cgctgcagga aactgtggcc taccgtgcct atgcggcagc
      661 gcagttgatg acgttcgagc gctacgcggg ctggtttttc tggagctacc ggaccgaaac
      721 cacgccggcc tggtgtttcc gcgactgtgt tgagcgcggc tggctgccgg atgacttcaa
      781 cccttgagcg ggcgctcctc cagccagcag acctgcgggt tgtgaaacag atacttctcg
      841 ccgcagaccc gggtcagcat gatcttgcgg tcatggtgcc gcgccagggt ttcgtgaaag
      901 ccgatggtca acagtgtggc gttgggcagt tcgcggtgca gcatctccat caggcaatcc
      961 tcggccttgg gggcgagggc gtcactcgcc tcctcgatga agatccaggc ggggcggtgc
     1021 agcagcagcc gggcgaagcc gaggcgttgc tgggtgcgca gtgccagcac atggtcccac
     1081 tgatcgctct cgtccagacg cgccgccagc caggagagcc cgacacactc cagcgcgtag
     1141 tggatgctgc cggtctcgaa gcggtcggcg ggcgccggat agctgagcag tgagcgcaat
     1201 gtgccatcgg gcaggaacgg acgttgcggc atgaagtaga tcggctggcc gcgtggcagc
     1261 agcacttcac cctctcccca tggccagagg ccggcaatgg ccttgaacag tgagatggcg
     1321 gcggtggcgt cgccatcgat ccagacccgt tcgccacggc ggaaacgttc gtcgagatgc
     1381 tcgatgatca atttgccatc cgggttggcg atgctgaggt cacgcagttg cagctcatgg
     1441 cgggccgatt tttgacgttt gatgtgtcgg ccatcgccct gtcggctctc ggcagcgagc
     1501 gccgacaggt catcatgcag cgacagcatc cgctcggcgg aagcgcggaa tttggccatg
     1561 tcgcccaggt tgtcgatcgg ccaggagagt gccgaagtca gtttctggaa cgcctgggcc
     1621 gactgcatca gcacacccag tgtcatgaca ccggcaatgt actgtggggc ggcaaccaga
     1681 atcgggaaga ccggcagcag cgcgccatag ccggaggaga aggagacgat gcccatgtag
     1741 gcaagggtct ggcgattcca gccctgcccg agagcatcga actgtcgcaa ggagtgttgc
     1801 cgttcgttct gttcgccctg catcagcgcg atggcctcgg aattttcacg cgagcgcgac
     1861 aggccgaagc ggaagtcggc ctccagggtc tgcaacctgt tggtggcctt gaccagtggc
     1921 cgccccagca gcaggccgag cgccgttccc gcgccggcgt agagcagggc gaggatcacc
     1981 atgtagcccg gcacctgcag cgaggtgcct ggcagactgg tgctgccgga gaccgacagc
     2041 agaatgtcga cgaaactgcc gagaatcagc agcgaataga ccagcgtgtg tgccagcatg
     2101 atcgccattt cggtggcgat gcggatgtcc tcggcgatgc ggctgtccgg gttgtcatgc
     2161 tcgccggcgg tgaactgcaa ctggtagtgg tgaccctgca ccatccactg gccgaggagg
     2221 cgctcggtca accagtgccg ccagccgatc tgcaaccagc gcttgacctg cagatggagc
     2281 gcggtgacca gcatcgtcag cacgaagatc agcacaaagg tgaagccatg gcgcagaaaa
     2341 agggccagtg agcgcgcttc cagggcatca aagagcgcgc ggctccagta gttggtccag
     2401 atcaccagcg ccacttgcgc cagcgtcaac agcagcagcg ccagcgtgat cgcgcgcact
     2461 ttccacttct gctcgctgga ccagtagggc cctgccagcc ggatgaagcg atgcagaaaa
     2521 aggtagcgcg aacggatcgg accgatcgcc ggcgagacag ggttgggtga agaatcgagc
     2581 gggggcatgg cggtcgtggt cagactctgc cgggtcttcg tttatcgtac atggccgacc
     2641 atgcagccga cagtgtggcg cgctcacgca tggcggttga gcgcatcgat ctggctgttg
     2701 agcgtccagt agtcgtaaag atagcccacg ccacccagcg cgaaggtgca gagatagagc
     2761 aggccggtcg gccattttcc cagatagaaa cggtgcacgc cgaacagacc gagaaaggtc
     2821 agcagaatcc aggccgtgct gtagtcgacg gcgccggtct gatagtgctc ctcggcttca
     2881 cgcgccatgg ctggaatcag caacagatcg atgagccagc cgatgccgag cagtcccagt
     2941 gtgaagaacc agagtgtgcc agtgaccggt ttgccgaaat agaagcggtg ggcgccgatg
     3001 aagccgaaaa tccacagcag atagccgatg gtttttgaat gggtcgctct ggactgcacg
     3061 aatgcgctcc tgttcttcgg ttttgttgca tcggtcgcag cgcatcagcg ccgcttcagc
     3121 catactgccc ggcatcgtcg ctctttgaaa gcgtggctgg cgtgcgtgtc gggcgttgaa
     3181 cattgttgcc gtgactgctg tccaacgtgg tgtctggagc cggtttccga cggggtgccc
     3241 agcgcgtgca cctctatatt tgaatgcggt tgatgacagt ttctcttttt gaaaggagct
     3301 ttgcatgaaa ttcgtcaatg gggtgttggt ctgtggtctg ctgtcgctgt cgatgtcggc
     3361 gctggcggga tcgaccacgg atgccgccat cggtggcgcc gtgggcggtg cggtcggcgc
     3421 ggcggttggc aatgaggtgg gcgggcgtga aggggccatc gtcggcggtg cgatcggtgc
     3481 cgccaccggc accgccatca ccaccaaagg ggatgagaag catgatggcc atcatgacca
     3541 cgatgatgac cattaccggc gcgtcgagcg ggaccatgat gacgatcatc accaccatgg
     3601 ttcatcgttc tgccctcctg gacaggcgaa gaaggggcgc tgctgaagtg ggcgcgctgg
     3661 cctatgcctg attgctgtcg gggtcatagg ccaggttggg gctcagccag cgctccagct
     3721 cactgatcgg catgcccttg cgctgggcca gcgcttcgat ctggtcgcgg ccgatcttgc
     3781 cgacgccgaa gtagcgtgag tcggggtggc tgaagtagaa gccgctgacg gccgccgccg
     3841 gggtcatcgc aaagtgctcg gtgagctgca cctggatgct ctgcgcggca tcgagcagtc
     3901 tgaacagcgt cgccttttcg ctgtggtcgg ggcaggccgg atagccgggt gccggccgga
     3961 tgccgcggta gcgctcgtgg atcagcgctg ggttgtcgag ctgctcatcg ggcgcatagc
     4021 cccagaattc gcgtctgatc cgcagatgca ggtgttcggc gaaagattcc gccagccggt
     4081 cggccagcgc cttgaccatg atcgcactgt aatcgtcgcc gaccgcctca tacttggcgg
     4141 ccagctcctc ggcaccgagt ccgccggtga ccacgaacag ccccaggtag tctgccacgc
     4201 cgctgtcgag cggcgcgatg aagtcggcca gactgaggca gggcgcatcc tccgggcgct
     4261 cgccctgctg acggatgaag tggaaggtgg ccagtcgctc ctgacggcgc tcgtcggtga
     4321 agagcactac atcatcgctg cccacccgat tggctggaaa caggccgaac atgccgttgg
     4381 cgctcaacag cccgttgacc atgatgcctt cgagcatctc ctcggcatcg gccagcagct
     4441 cccgggcatt ttcgccgacc accgcatcat cgaggatgtt cggatatttt ccgctcaact
     4501 cccaggtcat gaagaagggt gtccagtcga tggtgtcgat cagtttttcg agcggatagt
     4561 tgcgcagggt gcggatgccg agttcgttgg gtaccggtgg ccgatagcgg cgccagtcgg
     4621 tgacgaaacc gcgctgacag gcttctgcgt agggaatcaa ccgggctgcc tggttgcggc
     4681 ggccgctgcg ctcgcggatg gtggcgtact cctcgcgccg ctgggcgacg aaatcatcgc
     4741 gcaacgtgtc gctcagcagt gaggtgacga cgccgacact gcgcgaggca tcgggcacat
     4801 agacggcgag gtcgttctgg tagcagggct cgatcttgac cgcgttgtgc gccttggagg
     4861 tggtggcgcc gccgatcagc agcggcagtt tgaatccctg ccgctgcatc tccttggcga
     4921 catggaccat ctcatccagt gacggagtga tcaggccact caggccgatc acatcgacct
     4981 gctgctcccg tgcagcgtgc aggatgcgct cgcaggagac catcacaccg aggtcgatca
     5041 cctcgtagtt gttgcactgc aacaccacgc cgacgatatt cttgccgatg tcatgcacgt
     5101 cgcccttgac ggtggcgagc aggatccggc ctttcttctc gatgttgccg ccggccgatt
     5161 cggcctcgat gtagggcgtc aggtgggcga cggcctgctt catcacccgg gcgctcttga
     5221 ccacctgcgg caggaacatc ttgccggcgc cgaacaggtc gccgaccaca ttcatgccct
     5281 ccatcagcgg accttcgatc agctcgatcg gccgctcgac ctcctgccgt gccgcctcgg
     5341 tgtcctcgac gatgaaggtc gagatgccct tgaccagcgc atgaatgatc cgctgcgccg
     5401 gtggcagttc gcgccaggcg agctgctcgg cctctggccg gctgccgctg ccgccctcat
     5461 ggaggtactt ttcggcgatc tccagcagcc gttcggtgcc atcggcacgg cggttgagaa
     5521 tcacatcctc gacccgctca cgcagttcgg ctggaatctc ttcgtagatc gccaactggc
     5581 cggcgttgac gatgcccatc gtcagcccgg cgcgaatcgc gtgatagagg aagacggcat
     5641 ggatcgcctc gcgcaccggg ttgttgccgc ggaacgaaaa cgagacattg gagatgccgc
     5701 cggaggtgag cgcatggggc agatgggtct tgacgtaggc gcaggcctcg atgaagtcga
     5761 ccgcgtagtt gttgtgctcc tcgatgccgg tggccacggc gaagacattg gggtcgagaa
     5821 tgatctcctc gggcggaaaa ccgacctcat cgaccagcag ccggtaactg cgctgcatga
     5881 tctcctgctt gcgcgccaag gtgtcggcct gtccggcctc gtcgaaggcc atcaccacca
     5941 cggccgcgcc atagcgacga cagagccgcg cctgatgcag aaaggaggcc tcgccctcct
     6001 tcaggctgat cgagttgacg atgctcttgc cctgcacgca cttgaggccg gcttcgatca
     6061 cctcccattt cgacgagtcg atcatgatcg gcacccggct gatgtccggt tccgaggcga
     6121 tcagattgag catcgtcacc atcgccgccc tggagtcgag catcccctca tccatgttga
     6181 tgtcgatgat ctgcgcaccc gattcgacct gctggcgcgc cacttcgagt gcctcgccgt
     6241 agttctcctc gcgaatcaac cgggcgaagc gggccgaacc ggtgacgttg gtgcgctcgc
     6301 cgacattgac gaacaggctc gatgcgtcga tgaccagcgg ttcaagtccg ctcaggcggc
     6361 aggcggggtc gagctgtgga atcggccgtg gccggttccg tgccgccgcc tcggccagac
     6421 ggcggatatg gtccggtgtg gtgccgcagc agccgccgac gatgttgagc aggccgctct
     6481 gcatgaactc ctcgaccacc gccatcatct gttccggtgt ctcgtcatac tcgccaaact
     6541 cattcggcag tccggcgttg gggtgggcgc tgaccggcac atcggcgatg cgtgccagtt
     6601 cggcaatgtg cggccgcagc tcgctggcgc cgagcgcaca gttgaagccg atggcaatgg
     6661 gttcggcatg ggccaccgag tgccagaaag cggccggggt ctggcccgac agcgtgcggc
     6721 cgctggcgtc ggtgatggtg ccggagatca tgatcggcaa cgcgaactgc tgttcatcga
     6781 aatagcgcct gaccgcgaag atcgccgcct tgcagttgag ggtgtcgaac acggtctcga
     6841 tcaggatcag gtcgacgcca ccttcgacca gcgccgcagt cgcctcgcgg tactcctcca
     6901 ccagttgatc gaagctgatc gaacggtaac cggggttgtt gacgtcgggc gagatggagg
     6961 tggtctggct ggtgggcccc agaatgccgg ccacgaagcg cggccgcgac ggatcgcgcg
     7021 cggtgacggc atcggccacc tcgcgtgcca gccgcgcgcc ggccaggttc aattcggccg
     7081 ccagcgcctg caggccgtaa tcggcctgtg acacccgggt ggagttgaag gtgttggttt
     7141 cgatgatgtc ggcgccggcc tcgaggtagg cggtgtggat ctcgcggatc acttcgggct
     7201 gggtcagggt gagcagatcg ttgttgcctt tcagctcatg agggtggtcg cggaaccgct
     7261 cgccccggta ggccgcctca tcgagacgat agccttgaat catcgtgccc atggccccat
     7321 ccagcaccag aatgcgctgc ttcagtgcgg tgttgagctg ctcgatacgc gcggcacggc
     7381 tggtcatggt tctgcttatg tcctaattaa tcgaaggttt ttgtcgaaaa tgaaaggtgc
     7441 tattttaaca gaagtgacct ttttcgctgt gccgatctgc cgcacacggc catccacccc
     7501 gttcaatcca actggtgaca cccgatgacc ccctccctga ccattaccga tgcggcgcaa
     7561 gagtacattg ccgagctgct ggcgaagcag ggcggcgagg tcaacattcg cgtcttcgtc
     7621 gccgaccccg gcacgccgaa ggccgaaagc tgcgtcgcct actgccgtcc cggcgaagag
     7681 gaagcggatg atctgccgct ggaactgaga ggcttcaagg ccttcatcga ggcacgcagc
     7741 ctgccatttc tcgaagaggc gctgatcgac tatgccaagg accgcttcgg cggtcagctc
     7801 accatcaagg caccccatgc caagatcggc aggatcgatg ccagcagtcc actggaagac
     7861 cggatcaacc atgtgctcta tgtcgaggtg aaccccaacc ttgccgcgca tggcggcgag
     7921 gtgagcctgg ttgaggtggt cgatggttcg attgccgtgc tgaagttcgg cggcggctgt
     7981 cagggctgcg gcatggtgga tgccacgctg aaggaggggg tcgagcggac gctggtcacg
     8041 cagattcccg aaatcaccgc cgtgcgcgat gtcactgacc actcggtgcg cgaccacgcc
     8101 tactactgat ccaggctatg catggtccgc ccatccaggg aactgcaaac ccgtaaaacg
     8161 tgacggatgt ggctgagccg tatcagccgc tgtgaggtgg tgtggcgtct ggctgccagc
     8221 ccccacccag cgccttgtag agttgcacca gggcgcgcag accttgctgc tgagtgttga
     8281 tgcgcgcgag ttcgacattg aacaggctgc gctgggcatc cagcacctcc agataatcgg
     8341 agtaaccctc ttcgaagcgg cgttgcgcca gccgggccgt ctgttgcaag gcggccacct
     8401 gctgtgactg ggctgccagc cgttcactgc tggcgctgcg gcttttcagt gcatccaaca
     8461 cttcacgaaa tgcggtgcgc acggtctgct ggtagttgat cagtgcttgc tggcgtcgtg
     8521 cctctgctgc ctcgacagtc gcctgggtgc gcccgaaatc gatgatcggc ccggtcagat
     8581 tgccggcgaa ctgccagttt ttagccgagc cgttgaacag attgctcagg gcatcgctct
     8641 gattgccgaa cagaccggtc agtgacagcc gtgggaaata ggcagcatgg gcaatgccga
     8701 tctcggcatt ggcggcacgc aactgctgtt cggctgcgag aatgtcgggg cggcgttgca
     8761 gcagggtgga aggcagcccc atcggcactt ccggcagcgg tggcagctca tcgagggtat
     8821 gcccggtcgt gatcgtttgc tgcacgagtt cacggggggt gcgacccagc agcacgctca
     8881 gggcgttgat ctggagtgtg cgctgctgtt cgagcgcagg caattcggcc cgggcggcgg
     8941 ccagttccga ttcggcctgt cggaatacca gttgcgaagt ggcgccgttg cgatagcgtg
     9001 actcctgaaa gcggtaactg ccttcgcggg ttttcacggt gttccgggcg acgagcagtt
     9061 gttgatcgag cgcgcgcaga ttgaagtaac cggaagcgat gtccgccgac acggcgaggg
     9121 caatggcgtc ccggctggcc acgctcgcca gcagttgtgc ctgcgctgct tcgttggccc
     9181 gggccagcct gccccacaga tccagttcgt agttcaggac tgccgagaca ctcaaggcat
     9241 catggatgac accgccgccg ggttgggcgg tttccgcgct gccctgctgg cgcgaagcat
     9301 cggcctgtcc gctcagcagc ggatagcgct cggcatcacg caggttgagc agcgcccgtg
     9361 cttcggcgat ccgggccgcg gcaacggaga gatcggcatt gtgctgcagg gcctcttcga
     9421 tcagtgccgt caaggcgggg tcatcgaaga ggtgccacca atgtggatcg atcgtcggtg
     9481 tgacggttgc cgccgcagag tctgctgcgg gaaccatcca gccactcggc aggtcgctct
     9541 ggggttgcag atggtcagga cccagcaggc agcccggcag tgtcgaaagc agcaacagcg
     9601 gcatcaagag tctgttacgc atgcggatcg ctctccttct cgcgcgtgtg ggtgaccccg
     9661 tcgcgctcgg tcagtcgacc gatcagcagg aagaacatgg gcacgaagag ggtggcgaga
     9721 aatgtggcgg caatcatgcc accgatgacc ccggtgccga tggcgtgacg actggccgac
     9781 ccggcaccac tgctgatcgc caatggcaac acgcccagca tgaaggccag cgatgtcatc
     9841 acaatgggtc tgaagcgctg gcgggcacct tgcaatgcag cttccatcag cgacacaccc
     9901 tcgcggcgat ggagcatcgc gaattcgacg atcagaatgg cattcttggc cgacagacca
     9961 atcagggtga gcagaccgat ctggaagtag aggtcgttct ccaggccgcg cagccaggtg
    10021 gcgaggatgg caccgaacac ggcaaagggc actgccgtca ccaccgcgaa cggcaggctc
    10081 cagcgttcat actgggctgc cagaatcagg aacaccatga ggatgccgaa cacgaaggat
    10141 tgggccccac tgccggcact ggccagctcc tgataggccg atccaaccca gcccagcgag
    10201 tagttggcac ccagaacctc ctggctgacc gcctgcatgg cggcaatggc ctggccggag
    10261 ctgtagcccg gggcgggctg gccgagaatc tttgcggcag gaaagacgtt gaaacgctcg
    10321 accagatcgg ggccgatggt gcggttcacc ttgaccaggg catcgagcgg caccagattg
    10381 ccgtcgctgg agcggacgaa gacgtggcgc agatcctcgg gagagcgacg gaattgcgct
    10441 tccgactgca ggttgacgcg atagctgcgt ccgaacaggg tgaagtcgtt gacatagagg
    10501 ctgccgaagg tgctctgcat gatcgtgaac acctggttga tcggaacctt gagggcgcgc
    10561 gccttttccc gatcgagctc cgcgtagtac tgtggcgtgg aagtggagaa ggtggtgcgc
    10621 agttcagtca actccggacg tttgctggcg gaatcgagca gtcggtgggc cgtttccacc
    10681 agggcggttc cgccgctgcc ggtgcggtcc tgcagatagg cctcgaagcc cccggtggtg
    10741 ctgatgccgg tgatcggcgg ggggttgaag ccgaacatct cgccatcgcg cagatcggca
    10801 cccagatgcg cgagcggatt caccatgatc cgtgcatcgt cttcggaggt ggtgcgttga
    10861 tcccagggat cgagcatgac gaacgagatg ccggcattgc tgcgctgtga ctgggccaga
    10921 atgtcgaagc cggcaaaggt gaccaccgat tccaccgcct tctgctccat cagccgttgc
    10981 gtcatcacgt cggtgatcgc ccgggtacgg tcgagtgctg cggcagcggg caactgatag
    11041 gccatcagta cgtagccctg atcctcggca ggcaccagcc caccgggcag ccggttgaac
    11101 agcagcaggg caatggccat caggccggtg aacagcgtca tgcccagcac tgtgcgtcgc
    11161 atcagaaaac cggaagcatt cacatagcca tcggtgatgc gggcgaacat ccggttgaac
    11221 agccggaatg gcagggccgg ttcgctgtgc accggtttga gcagcagtgc gcacagcgcc
    11281 ggggtcaggg tcagtgccac gattccggag atgaccaccg agaccacgat ggtgatggcg
    11341 aactgcttgt acatctgtcc gctgagcccg ccgagaaagg ccaccggaat gaacaccgcc
    11401 gacagcacca gcacgatggc gatcaccgga ccggtcacct cggccattgc cttgaaggcg
    11461 gcttcgcgtg gcgacagccc ctcctggctc atcagccgtt cgacgttctc gatgacgatg
    11521 atggcatcgt cgaccacgat gccgatcgcc agcaccagtc cgaacagggt cagcaggttg
    11581 atcgagaaac ccagcagcaa catgccggcg aaggtgccga tgatcgagac tggcacggcc
    11641 agcagcggaa tcagcgtggc acgtggattc tgcagaaaca gaaacaccac cagcaccacc
    11701 agcacgatcg cttcgatgaa ggtgtgcacc acctcttcga tggagacccg gacgaagcgg
    11761 gtggtgtcga agggaatttc ataggccagc ccttcgggaa agcgctggga catttcgacc
    11821 atgctggcgc gaatggcatc catcgtctgc aacgcattgg cgcccggctg caggtagagg
    11881 ccgatgggga ccgtcgttct gcctttgtag ctggcatcga aattgtaatc ctgcgctccc
    11941 agttccaccc gcgccacatc gctcagacgc agtgtggcgc cggagtcatc cgagcgcagg
    12001 atgatctggc caaaggcggc cggatcggcc agcctgccct gggtgttgac ggtataggtg
    12061 aacgcattgt cggcggaaat cggctcctgg ccgaattgcc cggcggcgaa ctgggagttc
    12121 tgctcctgaa tcgcctgtgc cacgtcactt gcggtcaggt tgaactgcgc cagtttgtcc
    12181 ggccgcagcc agatgcgcat cgaatagtcc ttctgaccga acagcgatgc gtcgccgaca
    12241 ccgggaatcc gcttgagctc gtcgatgatg ttgagcagcg cgtagttgct gagaaacatc
    12301 gagtcatact ggccgcccgg cgcagagaca gtgatcaacc ccaggatgct ggtggagcgt
    12361 ttctgtaccc gtattccttg tcggcgcacc tcctcgggca ggcgggcctg tgcggcggtc
    12421 acccggttgt tgacgttgat ggtggcctgg tcgggatcgg tgccgatggc aaaactgacg
    12481 gtgatgcgca actggccact ggcagcagcc accgaacgga ggtagatcat gtcctcgacg
    12541 ccgttgatct cctgttccag aggcgcggca acggtcttgg cgatgacatc ggcgctggcg
    12601 cccggataac tggtgatcac caccacttcg ggcggaacga tgtcggggta ttgcgcaacc
    12661 ggcaaggccc gcatcgacac cagaccggcc agcacgatga cgatggagag aaccgaggca
    12721 aagaccggac gacgaatgaa gaatgcggaa atcatggtct ggttccggac tggcttgcct
    12781 gattcgccac cggtgccggc gtcggtcgag gttctgcagg cttgaccggt tcgccggcct
    12841 tgaccttgat gacgccatcc acgatcacct tgtcacccgg tttcaatccc tcttcgatca
    12901 gccactgcga accgatggtg atccccggtt gaatcggctg gactctggcc cggtcaccct
    12961 cggcgagcag atagacgtag gcgccctgcg gaccctgcat caccgctgcc tgtggcaaca
    13021 ccatcacccc tttgcgcacc agccctttgg gtacgatgcg cacgaactgc cccggcagga
    13081 tgctgttgtc aggattggcg aacagtgcgc gtgcccggac cgtgccggtg gccggatcga
    13141 tgctgctgtc ggtgaagctg atgtagccct gttggtcgta caccgtgcca tcactgaagc
    13201 gcagctcggc cgccaggcgt tcgcccccgg tcatcgtcat ggcgccgctg gccagcagtt
    13261 ggcgctgacg cagtgcatcg gtgtcggggt aggagaaatt gacgtagagc ggatcgagct
    13321 gggtgatgcg ggtcagcagg ctgtccgcgc tgcccgggcc caccagactg ccttccgagc
    13381 gcacctccag actggtgatg ccactcaccg gcgccaccac cttggtgtag ccgaggtcga
    13441 tgcgggctgc ctcgacatca ctgttggcca ccgccaggtt ggcgcgggcc agatcgagtg
    13501 cagattgcac actgtcacgc tcgcgcgcgc tgatggcatt ttcggcaaag agcgcggcga
    13561 cccgtgtcca atcccgttcg gcctggcgca gttgggcttc ggcatcgctc aatctggcct
    13621 gggcgcgggt cagtgccacc tcgaacggag cagggtcgat gcggaacagc agatccccgg
    13681 ccttgacctg ctgtccctcg acataggtcc gcttctgcag gatgccggtg atgcgcgccc
    13741 gcacctcgac ttcgcgaaag ccggcggtac gcccggcgta ctcgttctcg atcggtacat
    13801 cacgcggttg cacgaccatg atgcccactt cgggcgttgg ctgtgcatgt gaattgccat
    13861 cctgctttct gtcgcaggcc gtgagagtgg tcagcagcag ggtgcagagc agggcagtgg
    13921 gcagatgccg cgttgggagc cgggtggcag ggcgtatcat tcagactctc tcctgcagtc
    13981 ggtggaagca tcgatcctga agcgaattga gaccgcagaa aatcggcaag gcagacaaaa
    14041 agtcttacaa tacatgcgga aacaggcggt ggaacaacgt gcttggatgc tggaaaatta
    14101 catacattca agtatgtttg tcaacaacaa taacaggcgg atcgccttcg aaggagattt
    14161 gaaccatggc acgtcgaacc aagatggaag cggaacaaac ccgcgaacag atcatcgatg
    14221 ctgccgaaga gctgtttttg acgcgcggcg tggcgcgggc cacgctggag cagatcgcac
    14281 aggccgcggg agtgacccgc ggtgcgatct actggcattt ccgcaacaag accgatctgt
    14341 tcgacgccat gtactgtcgg gtgcatgatc cggtcgagga tctggtcagc cagtcgctgg
    14401 cgctggaaga tgaaggggcc atcgaggcgc tgcgaaattt ctgtgtgcaa tcactgctca
    14461 gtctggcgcg tgaccgacgc cggcggcggg tttacagcat tctgttccac cactgcgaat
    14521 tcgtcgatgg cttacaggag agcaatcagc gtctgatcgc cattcagcac cgtggccttg
    14581 ccagcatgga agcgctgttc cgccgtgcac gcgaacaggg cagtctgccc aacgtctgtc
    14641 cacggctggc ggcgattgtc atccacacct cgatgctggg actctacttc gactatttgc
    14701 gggagccgga tgcctatgat ctggagcggg aggcagcgcc gctggtcgat ctgattttcc
    14761 gctgcatcac ctgaacgggc gcatggtctg atcagaccgg tgagcggcct gcctgcacga
    14821 tggctggaga atgcatcggc cttcagggtg tggcaacgtc agccgtttcg cgaaacaggg
    14881 tccggtttct gccgctgcgt ttggcctgat agagtcgctg gtcggctgtg cggaacagct
    14941 cggcgaagct ctctccatcc gcaccactct gcgccacacc gatgctgacg gtgacttcaa
    15001 tggtggtgtc gcgatggcga atcggcatct gctgcaacgc cgttcgcacc cgttcggcca
    15061 ccgaccaggc ctgcgcttcg gtgctctcgc gcagcagcac ggcaaactct tctccaccga
    15121 tacgccccgt caggtcggat ttgcgcaact gctggttcag caacagcacg aattgccgca
    15181 acacctcgtc accggcctca tggccatgtc gatcattgac ctgtttgaag tggtcgaggt
    15241 cgagcagcag catgaagttg ctgctggctg tgcgcgtcag cagtattttt tcggccatca
    15301 actcgaaccc gcgacgattg aggataccgg tcaggccatc ggtgatggcc aatctgtttt
    15361 ggcgggcgag aaacttttgc gacaccagca acagcagccc gacgttccag taaccgaagc
    15421 tggcaatcag caccagccag gtgagtgacg acagcggtga ggcttgcagc aggctcgatt
    15481 cggttccgat cctgatgatg gaagaagcga ggaggtgggc atccatgatc accagcaacc
    15541 ggaccgtgaa gagcagggcc agcagggtga acagcgtggc gttgacccac cggggcgcgg
    15601 gctggatttc atgctcggtc ggcaggaaaa actcgcgcgc aatcaggatg cacagcacga
    15661 tgaatggcaa ggagaacagc aggacgcgga tggcgtaatc gggactgatc tgaccgaaat
    15721 ggaaaaatgc cagacagctc agaacgaaaa ccaccgccgt tgcccgcagt gaagtcgggc
    15781 gctgatagaa gcagcgtatc gcatgatgca aaaaggtcag accgctcagg atgctggcat
    15841 tggcgatcac catggagagc agatcgggaa tcagcccgcg tgcggcgatc aggatcagac
    15901 cgagattgat cagtgcatgg gcgatgacaa acttgccgag gccgggttcg tcacggttgt
    15961 tcagatagat gaagccctgt gtcagcgtgc acatcaggta gatcagcgcg gtgaccagaa
    16021 tcagcgtgcg gatgtcgagg gtggcgaaaa tctctggact gggcattggg tgtcgggggt
    16081 gcgcttcaat cggtcaggtg tcggcatgac cagatgttca atcaagcata gcctcattat
    16141 cgccccaagg ccgcaacgag cccggatgga caactcatgg aagccggtag gcagccagcc
    16201 ggttgatggc catacggatc tccgattcga tctgctggtg gcgtgtggtg tcgaaaccca
    16261 catgagtgcc ctccttgggc agcgttttca gtgccggcat cagcgtgccg tcatcggagg
    16321 gcaaagcgct gacgacctgc tgcaactgcc gggcgccggt ttcatcatcc gtcgccagcg
    16381 tcgccagtcg aatgccggcc agatcagcgg caaagtcgag gtagctgaag tcgttgcccg
    16441 actcctgccg gtcgttcagc tccttctgca gaccgatcca ctccgccgtc tgcggcaggc
    16501 tcttgagggt gatgagcgcg gagagcaggt agtgctgcgc aaggtctgaa cgcctgtgca
    16561 gcgtcagcgc gaccgggtga tcgcggtcgc gcacgaaggg ttcggccatg ccggccgcgt
    16621 agagggccag cgcggtgatg gcggcgcggt tctcgccttg cgccgagcct gcggcaccac
    16681 tgcgctccat cgccagtcgg aacagtgccg gcagcgccgt ggccagactc aatgcgtcat
    16741 cggtctggct gatcaactgc cgcagccgtt cgttgtaggg cgccacggct gcatggtcga
    16801 tgccctgcca gagccagcgg ccaccctcgg cgacgatgct gcgcacctgc tgtcgatcga
    16861 tgtggtgatg gagcgtcagc cggtcggcgc cgatttcgat gcggtcgatg ccgttcaggg
    16921 tctgttgcag aaaatcgatc tgcggccaga gtttcgtgcc ctcgcgcagc cagccgttga
    16981 gctgtgcaag cgagagcgcc ggcagtggca gggcgccgag ccggcagcgg gtggtcagaa
    17041 tctccagtgg cgtggcggtg acgcggcaga tcaaattgag gtggcgcgct ggccagaacg
    17101 acagcggcgt gctggccaga aggtggagcg tgcccggttc gatgcgcacc tggctggtgg
    17161 cctgccgggt gcccggcaga tggcccagtg ccgccctgaa gaacaggtcc agttcactgg
    17221 cctgcagcgt cagcgcctgg ttcgacctgg tggccgattg cttgattcgc tgcgggaagg
    17281 gggcaatgtc ggccgtgggg cgtgggcggt cgggcatcgc cagtggcgcg ggcgcggtgg
    17341 ccagccacag cccggccgcc agcagcagag cgaggagcgc aaccagcaga cgcagcatcc
    17401 gttcagcggc ctgccgcact gccttcacgg cgtgaatcgg cagcgccaat cagcccggtc
    17461 gggttgcgtt cgatcagatg aattccactg ctgaggtcgc gcacatcgac cggatggccc
    17521 agtgcttcca atggttcgcg cagcgtgacg gcggcggtgc ctgcctccag ttcggtgcgg
    17581 tcgttgcgat tctggtagtg cggcagatcg acggtgcgtt gcggatcgag ccgccagtcg
    17641 atcagtgcga tcaggctctg ggccacgaag gggatgatgc gtgcgccacc cggcgagccg
    17701 aggatccatc tgagttcacc ctgttcatcg aacaccatgg ccggtgtcat cgaactgcgt
    17761 ggccgtttgc cgccggcgat gcggttggcc actggctgac cgttctgttc gggcaggaac
    17821 gagaagtcgg tcatctgatt gttgagcaga aagccatcga ccatcagcgt cgagccgaag
    17881 gcggtctcga tgctgctggt catcgacacc gcattgccat cgcggtcgat gatgctgaag
    17941 tggctggtgg agggaagttc gggtgaacgg tcttcggcct gggtcagcag cgacagctgg
    18001 cccgccgccg ccacgcccat gtcatgttgc gggttgatca gccgggcacg ctggcgcaga
    18061 tagtcggggt cgagcagtgc cttcagcggt accggaacga aatcgctgtc ggccagatag
    18121 agcgcccggt cggcgaaggc gagccgtccg gcctgggcaa agagatgcgc cgcctcgacc
    18181 gaaaccggcc tgaatgcggc caggtcgaag ggctccagca gtgccagcat ctgcaacagg
    18241 gtcacaccac cggaagaggg tggtcccatg ctgcagatcc gatgaccgcg ataggtgccg
    18301 cacagtgggg gccgctcctt cggctgatag tgggccaggt cggtgaggcg caaggggccg
    18361 ggaatgtggt gcgcctgtac ggcgtcgacc atgcgttgcg cggtttttcc acgatagaag
    18421 gcagtggggc cttcgatggc gatgcgccgc agcaacttcg cgtaggccgg gttggtcagc
    18481 cggctgccgg ccgtgagcgc ttggccagca gggtagaagt agtcatgcgc cgctccgggc
    18541 tccagcaggc ccggattgct gttcttttgc agcagttcgg ccagtcgtgg cgagacgatg
    18601 aagccccggt cggccagacg gatggcatcg gtgaacagtt gccgccacgg cagccggcca
    18661 tggcgctgat gggccagttc gagcatggcg agcacgccgg gggtgccgac cgagcgaccg
    18721 ccgacgatcg cctgcagcat cttcatcggg gcgccatcat tgttcagaaa cagatcgggc
    18781 gttgcggcag caggtgcggt ctcgcgcccg tcatagctga tgagctgccc ggttctgtgc
    18841 gtccagtgga gcatgaaggc gccaccgcca atgccggagg attgcggttc gaccagattg
    18901 agcaccatct gcacggcgat ggctgcatcg actgcggaac cgccggcggc gagaatgcgg
    18961 cggccggcct cggcggcctg tggatcggcg gccactacca gatagcggct ggcggtgacg
    19021 gcgctcttgc tgcggctgcc agtggcggct tccggagcgc ggttctcatc gacggcccag
    19081 ctgcggccgg cgccaccgag caatatcagg ccacagagca gcaggcgcgc tatgatggtc
    19141 tgccgagcga cgacaggaga gagcgacatg atagaccttt acaccgcagc gacgcccaac
    19201 ggctacaagg cgtcgatcat gctcgaagag attggcctcg actataacgt aatcgccctc
    19261 aatctggcag ctggcgagca gaagagcccg gctttcctgg ccatcaaccc caatggccgc
    19321 atcccggcga tcgtcgatcg ggatgaagag gatttcgcca tcttcgaatc gggggccatt
    19381 ctggtctatc tggccgagaa gagtggccaa ctgctgccga ccgatcgcaa ggggcgctcg
    19441 agggtgatgc agtggctgat gttccagatg ggtggcatcg ggccgatgca ggggcaggcc
    19501 aatgtcttct tccgctactt cgaagagaag ctgccggcgg tgatttcgcg ctaccagaac
    19561 gagacccggc ggctctacca ggtgctcgat ggccaactgg cgcagcatga gtacctctgt
    19621 ggtgactaca gcattgccga catcgccaac tggagctggg tgcgtgcgca caactgggcc
    19681 ggcgtggcga tcgatgacct gccccacctg cagcgctggc atgacaccct gcaggcacgt
    19741 ccggcctgtg tgcgcggcat cgccgttcca ccgcgaccac cccgctccga tgccaccatt
    19801 gccggcgcgc gacagatgtt gacccgctag gtgcggctat ggcctgatca ggcgaaaggt
    19861 cagattgatc cgcggttcgc tcacgcctct gctgcgtggc agcgcatggt gccagtgtgt
    19921 ttgcagtggt ccggccatca gcagcagtga gccgtcggtc agcagaatct cgattcgcgg
    19981 tgggtccttg cgccggcggt gtcgcagcag aaaccggcgg ctggcaccga agctgaccga
    20041 ggcgatggcc ggatcgacgc ccagttcggc ctcgtcgtca ctgtgccagc ccatcgcatc
    20101 gttgccatca cggtagagat tggccagagc cgagttgaag cgcaacccgg tcagggtctc
    20161 gacctgccgg cgaatttcgt tcagcagtgg ctgccagggc gttggctgca acagcagccc
    20221 cgaataacgg tagctcgcgc ccggatcgcc ataccaggcc tgcaaccggg gcagccgatg
    20281 acgcttgccg aacagcgtga tctcatcctg ccgccaggga atggccgagt tcaactgctc
    20341 gaacagcgat ggttcgtcgg gccatgacag gacaaaggca tcgggccaca acgtgagctc
    20401 gccatcgtcg atgacaattg accgggctgc tggaggggag gtcatgggga gtgattccag
    20461 caggtgcgcc gctgcacacc ctgggtggtc atcttgatgt catctcgaaa acggaacatc
    20521 ggaggcgttc aaggacaatt ctccgccaga gccgtgtgca gcgacaacca cgctt
//
LOCUS       DAORCQ010000037        19495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1655_length_19495_cov_5.652058, whole genome shotgun sequence.
ACCESSION   DAORCQ010000037 DAORCQ010000000
VERSION     DAORCQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 19495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19495
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_19495_cov_5.652058"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKI23_05625"
     CDS             <1..789
                     /locus_tag="PKI23_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNN36452.1"
                     /translation="SFGLDPVFVPDLSGSLDGHVPEDFSPTTIGGTRREAIAALGSAG
                     LVLAVGEQMRTAAEWLGQHIDAPVQLFDRLLGLGATDALMAFLAQHSGRPVPGKYRRQ
                     RSQLVDAMLDGHFYTGGCKVAIGAEPDLLHGVGQFLAELGCELAAAVTTTDSPLLERL
                     PTEEVLIGDLEDLEARAAGCDLLITHAHGRQMAQRLDIPFLRMGLPIFDRLGAAHRLA
                     LGYRGSRDFIFEVANALIAHGHEPHPDDWRTSDAPEAGAAAAVA"
     gene            830..1240
                     /gene="nifX"
                     /locus_tag="PKI23_05630"
     CDS             830..1240
                     /gene="nifX"
                     /locus_tag="PKI23_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifX"
                     /protein_id="HNN36453.1"
                     /translation="MKIAFCTQDLQRVDAHFGWAKNIAIYEVNPTSHQFVEAIQFDGD
                     LQEDGNEDKLAPKIEAIKDCAILYVAAIGGSGAARVVAHNIHPIKVAQPEPIAELLEK
                     LRDVLAGTPPPWLRKVMFKGESRQFDFDDEVQHD"
     gene            1233..1706
                     /locus_tag="PKI23_05635"
     CDS             1233..1706
                     /locus_tag="PKI23_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HNN36454.1"
                     /translation="MTESQPTATATTPMHSPFIIELVKQWRAQDAHGAWEGKSDADLL
                     APYLLTKEQRRQLPIIGDPDPETLWRVELFYNAVGLAIERATGVMVSPIMKLHHEGFG
                     RMVLTAGRLIVVNKQLRDLHRFGFESLEKLAAEGEKLVAAGIGMIGQYPAVANYG"
     gene            1749..1946
                     /locus_tag="PKI23_05640"
     CDS             1749..1946
                     /locus_tag="PKI23_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HNN36455.1"
                     /translation="MSDPEQLKARIKKLNAKATSLKMNLHDLSEELPIGWEKIPLIAA
                     EAHEAYRALAEARSELERIGG"
     gene            1951..2271
                     /gene="fdxB"
                     /locus_tag="PKI23_05645"
     CDS             1951..2271
                     /gene="fdxB"
                     /locus_tag="PKI23_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HNN36456.1"
                     /translation="MSDFSVTLPNGHAWTPRFVQTLNETRCIGCGRCYRVCGRDVFEL
                     VGLTEEGERIVVKMTAADEDDDDDVEYERKVMTIAHQENCVGCEACSRICPKQCHSHA
                     PLSL"
     gene            2310..2876
                     /locus_tag="PKI23_05650"
     CDS             2310..2876
                     /locus_tag="PKI23_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HNN36457.1"
                     /translation="MTSIAHITPAPPSAELLAAATRPVAPLTLVYAGVLARFGTLALP
                     MSQLQPLFDSHFPRASALLPRFDGELPAQLRCRFDEVEDVRDLLYEHRSNDSNDSRWL
                     AHAMAVGCLGSDHLWQDLGLPERRALSQLLQEHFTTLHARNGNNMRWKKFFYKQLCER
                     AELHICKAPSCAECIDYPLCFSAEAESA"
     gene            2873..3751
                     /gene="modD"
                     /locus_tag="PKI23_05655"
     CDS             2873..3751
                     /gene="modD"
                     /locus_tag="PKI23_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HNN36458.1"
                     /translation="MTFTHPPCALDDGELDRILGEDVGHGDLTTAALGIGARPARIDL
                     SARDAMVVCGVEEAARLFSRNGCQVATLIERGSRVERGTLLLAAEGRADALHRVWKSA
                     QTLIEHAAGIATATAQMVRAVAGIGKTPVVVCTRKNFPGTRAIAALAVQAGGAQMHRL
                     GLGETLLLFPEHRQFIAPAALAETVHRLRQRHPEKKLIAETGDSDEAVALARAGVEIL
                     QLERFSPERLAELLRTLNHLALPCLVAAAGGVTLANAAAYARAGADILVSSAPYFAAP
                     AEVQVCFTPIDRSSPG"
     gene            complement(3821..4108)
                     /locus_tag="PKI23_05660"
     CDS             complement(3821..4108)
                     /locus_tag="PKI23_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNN36459.1"
                     /translation="MSRVEEKLFQNRYRVDSGRPHINILDASVCASQCEEKPCTHCCP
                     ASCYTLEGDGRVTLITDGCLECGTCRIICTEFHNVEWNYPRGGYGILFKFG"
     gene            complement(4105..5403)
                     /locus_tag="PKI23_05665"
     CDS             complement(4105..5403)
                     /locus_tag="PKI23_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNN36460.1"
                     /translation="MNEKFDAIVVGAGPSGNAAAYTLAKAGLKVLQLERGEYPGSKNV
                     QGAILYADALERIIPDFRDEAPLERHIIEQRGWILDDAAYVGTSYRSNDFNTPPYNRY
                     TIIRAQFDKWFSKKVQEAGALVICETTVTELHMDGQQVVGVKTDRLGGSIYADIVILA
                     DGVNSLLAKRAGFHPEIKPQDVALAVKEIHFLPEETIQSRFNLSGDEGVVIEMVGKIT
                     QGMVGTGFLYTNKESISIGVGCLLSDFKRQKISPYTMLEEMKAHPGIKPLLEGSEMKE
                     YTAHLIPEGGYNAMPKLYGDGWMIAGDSGMFVNGVHREGSNLAMTTGQLAAQTVIELK
                     SENKPLNQKNLARYRQKLDASFVMKDLKKYRRLPHVVEKNQQFFTTYPELINRAAHTL
                     IAVDGIDKRTKERAIRRNFVMKRSRLGLVSDAFKLWRAFE"
     gene            complement(5435..6532)
                     /locus_tag="PKI23_05670"
     CDS             complement(5435..6532)
                     /locus_tag="PKI23_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNN36461.1"
                     /translation="MSQATPAAAESRKAGRGKFELDERLAAYKGVWVFIEHERGAVHP
                     VSFELLGEGRKLADQLGVDLAGVVLGAPGEAVRGFCAEAIQHGADFCYLVEDPVLADY
                     RNQPYTRALTDLVNHYQPEILLLGASTLGRDLAGSVATTLRTGLTADCTGLAIDTEKR
                     SLAATRPTFGGSLLCTIVTLNYRPQMATVRPRVMRMPERDSSRSGPVVEHPLSLIESD
                     IVTKVLEFIADDSSEQSTLPFADIIVSGGRGLKRPENFELIWDLARVLGAEVGATRPV
                     VQAGWVSADRQVGQSGKTVRPKLYIAAGISGAVQHRVGMEGADAIIAINQDPNAPIFD
                     FATYGIVGNAMQILPALTEAFRQALTSRMAG"
     gene            complement(6543..7391)
                     /locus_tag="PKI23_05675"
     CDS             complement(6543..7391)
                     /locus_tag="PKI23_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNN36462.1"
                     /translation="MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPAIINPYDMVALEE
                     ALRLKDRYKARVTVLTMGPPQAEVALRKALSLGADEAILLTDRAFAGADTLATSYALA
                     AAIRQIQRDMPVDLLFAGKQTIDGDTAQVGPGIAIRLGLQLLTYVSKIESLDLERREI
                     SVQRRAEGGVQRLQTRLPCLVTVLEGINELRFATMDDMFRAARHEIKVWNKEAAAIED
                     LNMIGLKGSPTVVSKVFAPTAQTQRAEIIDVQSGQPSDLAATLISKLFTRYPQLHGEI
                     TRRALS"
     gene            complement(7393..7749)
                     /locus_tag="PKI23_05680"
     CDS             complement(7393..7749)
                     /locus_tag="PKI23_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HNN36463.1"
                     /translation="MTTSLEQLLDQLNQLSAAEEFFNHLDVAYDPGVLHVNRLHILMR
                     FQQYLKRATAELGRLEEAAALKQACAALLARAHDDFVRSNAATEKVFKVFQEAEGGAV
                     SLDTLRRTLPSRAREV"
     gene            complement(7779..8525)
                     /gene="cysE"
                     /locus_tag="PKI23_05685"
     CDS             complement(7779..8525)
                     /gene="cysE"
                     /locus_tag="PKI23_05685"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNN36464.1"
                     /translation="MSDAALPRPRLFRRLREEIDCVFSRDPAARGRFEVLTTYPGLHA
                     LLIHRLAHRLWCGHWRYLARWLSFVARALTQIDIHPGATIGRRLFIDHGCGVVIGETA
                     EIGDDVTLYHGVTLGGVSASGGKRHPTLADGVLVGAGAKILGPITIGHHARIGANAVV
                     TQDVPAEATVVGIPGRVVLPIDRRRITRQGIDLDHHLMPDPVGKALACLLERIDRLET
                     ALLPNPAADGSDAQCGSCAVDCQDGGEQQG"
     gene            complement(8531..9691)
                     /gene="nifV"
                     /locus_tag="PKI23_05690"
     CDS             complement(8531..9691)
                     /gene="nifV"
                     /locus_tag="PKI23_05690"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HNN36465.1"
                     /translation="MSEPTLAIDRRVIINDTTLRDGEQTASVVFTLQERLAIARALAE
                     AGVPELEAGIPIMGGEEREALAAIAALRLPVRLMAWGRMCAADIAAAAACPVQILNLS
                     IPVSDFHIEHKLARDRDWVLAQIAQLVPRALDLGFEVSVGGEDASRADPDFLLRVAEA
                     AQAAGASRFRFADTLGLLDPFATLTHVQRLVRVVDIGIEMHAHDDLGMATANTVAAVL
                     AGASHINTTVNGLGERAGNAPLEEVVMALRVIHGHETGIDSRAFPAISQLVSLASGRP
                     VSANKSIVGAAVFTHEAGIHVDGLLKRADIYQGFDPEVVGRTHTIVLGKHSGGSAVRR
                     AYQQLGIVLDERQTGQMLSRVRDHASATKQAPTPSELKRFYLESSPCVTLHG"
     gene            9705..9992
                     /locus_tag="PKI23_05695"
     CDS             9705..9992
                     /locus_tag="PKI23_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36466.1"
                     /translation="MNEETQQGFSNDHAMRRISVFVFLFHHVAQMAPFRRPTNVVIVA
                     FATVAAVDDAGALRKLSGPAPLHCGEVVTEGEPHEDRAGPVCGTTWNLALP"
     gene            complement(10022..10360)
                     /gene="fdx"
                     /locus_tag="PKI23_05700"
     CDS             complement(10022..10360)
                     /gene="fdx"
                     /locus_tag="PKI23_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNN36467.1"
                     /translation="MPTITLLPHEALSPRQISFEATPGARLSDLLLRQGVALPHACEK
                     ACVCTTCHLIIRQGFDSLPMAKEEEEDQLGKAWGLEACSRLGCQARMGQQDLVIEIPR
                     YTLNFVHESG"
     gene            complement(10405..12273)
                     /gene="hscA"
                     /locus_tag="PKI23_05705"
     CDS             complement(10405..12273)
                     /gene="hscA"
                     /locus_tag="PKI23_05705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011103546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNN36468.1"
                     /translation="MALLQIAEPGRSTAPHSQRWVIGIDLGTTNSLVATVRNGAGELL
                     SDEAGRVLLPSVVHYRSDGSSVVGHAAQAHQRSDPCNTLVSIKRFMGRELDDLSMAAR
                     RHYRFVDRPGMVRIETAAGVKTPLEVSADLLQALRLRAEQRLGGELFGAVITVPAYFD
                     EAQRQATKDAARLAGIDVLRLLNEPTAAAVAYGLDQSVEGLYAIYDLGGGTFDFSLLR
                     LHRGLFEVLATSGDSALGGDDFDLCIYQWMLSQVNLARLDAGDSALLLAKAREAKEAL
                     SSAAGVVIDATLADGTLLGFMLERATFDELCAGLVERTLLPVRRALRDAGVGTHEIQG
                     VVLVGGATRMPAIRQAVARLFGQQPLTRLDPDTVVALGAAIQADALAGNRTDGWLLLD
                     VTPLSLGIETMGGLVEKIIPRNSTLPTARAQEFTTFKDGQSAITLHVLQGEREQVELC
                     RSLARCELRGIPPMAAGAARIRVTFQIDADGLLTVSACEQHSGVTTEVLVKPAYGLDE
                     QEVVRMLRDSREHAREDAAVRMLREQKLEGERLLEAARSALGSDRALLSPDEQRLIEQ
                     QMQRLEAALATAERAGITQCIELLQRETDEFAARRMNAAIHQALAGRHAAELAAEL"
     gene            complement(12277..12837)
                     /gene="hscB"
                     /locus_tag="PKI23_05710"
     CDS             complement(12277..12837)
                     /gene="hscB"
                     /locus_tag="PKI23_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNN36469.1"
                     /translation="MNALTQQGAAQRSAVADDHFALFGLPVRFQLDSVRLAAAYRQLQ
                     SQVHPDRHAGGSVTEQRLALQWATRANEAYQTLRNPLERACYLLRLRHGMVALSESAV
                     LPANFLLQQMDWHEQLGQARRQRDGAALGQLQTLLCAERFALHGQLAVRLDVEGDAAG
                     AAELVCKLNCIHKLITAIDEARLALE"
     gene            complement(12834..13157)
                     /gene="iscA"
                     /locus_tag="PKI23_05715"
     CDS             complement(12834..13157)
                     /gene="iscA"
                     /locus_tag="PKI23_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNN36470.1"
                     /translation="MSITLTESAARQVRRFLDQRGAGIGLRIGVRPSGCSGMSYLLEF
                     VDLASPDDLCFESRGITLLVDPGSLVYIEGTELDFVREGLNEGFAFNNPNAANHCGCG
                     ESFTL"
     gene            complement(13175..13543)
                     /gene="erpA"
                     /locus_tag="PKI23_05720"
     CDS             complement(13175..13543)
                     /gene="erpA"
                     /locus_tag="PKI23_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNN36471.1"
                     /translation="MQTEVVDPTLASPAAQLMFTERAASKVREMIAEEGCPELKLRVT
                     VSGGGCSGFQYGFSFDDQADEEDTAFDRHGVQLLIDPLSLSYLVGAEIDFHDGLDGSR
                     FVIKNPNAKTSCGCGKSFAT"
     gene            complement(13555..13989)
                     /gene="iscU"
                     /locus_tag="PKI23_05725"
     CDS             complement(13555..13989)
                     /gene="iscU"
                     /locus_tag="PKI23_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNN36472.1"
                     /translation="MAYSDKLMDHYENPRNVGVLDQEDASVGTGMVGAPACGDVMRLQ
                     IKVNEVGIIEDARFKTYGCGSAIASSSLVTEWLKGRTLSEAMQIKNREIADELALPPV
                     KVHCSVLAEDAIKAAIEDYQSKRPAGVETAGATEITRLTAAS"
     gene            complement(14023..15258)
                     /locus_tag="PKI23_05730"
     CDS             complement(14023..15258)
                     /locus_tag="PKI23_05730"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNN36473.1"
                     /translation="MRSPNAPTSSKQPIYLDYAATTPIDPRVVAKMLPFLTGNFGNAA
                     SRSHRLGWQAEEAIEAARAEVAALVNCEPREIVWTSGATESDNLAIKGAAHFHQNRGR
                     HLLTVKTEHKAVLDSFRELERFGFEVTYLAPQSNGLIDPQQFAEAIRPDTTLASVMHV
                     NNEIGVVQDIASLGAICRQHGVLFHVDAAQSTGKVGIDLAELPVDLMSFSAHKSYGPK
                     GIGALYVRRKPRVRIEAQMHGGGHERGMRSGTLATHQIVGMGEAFRLAKQEMAADSAR
                     ITALRDRMLAGLMQIGEVHLNGDPQQRVPHTLNVSFNFIEGESLMMAIKELAVSSGSA
                     CTSASLEPSYVLRALGLSDELAHSSIRFSLGRFTTAAEIDHALVTVRAGVERLRALSP
                     LWDMHQQGIDLNSVSWAAH"
     gene            15612..17255
                     /gene="nifA"
                     /locus_tag="PKI23_05735"
     CDS             15612..17255
                     /gene="nifA"
                     /locus_tag="PKI23_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HNN36474.1"
                     /translation="MVTASNGGKPMGNVARSRHEAVASQLLAIYEISKVLGSSLDTEK
                     SLRGVLNILSAHLRMRRGMVCLRQPSGGFYIAGAPDLTSQEIERGKYRSGEGVIGQII
                     KSGMPIVVPDIRLEPRFLNRTESRDFSADQVIAFLGVPIQAGNELLGALAVDRETEDA
                     LSTTHFERDIRFLKMVANLVGQMIRLHRNIAAEREELLVEKSRLQKELRGKYSLDSVI
                     GQSRRMQEVFAEVHQVAPSRATVLLRGESGTGKEVIARAIHYLSPRKDKPFIRVNCAA
                     LTESLLESELFGHEKGAFTGATSDRKGRFELADGGTLFLDEIGEVSPAFQSKLLRVLQ
                     EREFERVGGNRPIHVDVRIITATNRNLEEAVSNKEFRADLYYRINVVTLALPALRERR
                     EDIPLLAAFFLDKFNRENQRNLTFSAEAVRTLLACNWPGNVRELENCIERTATMTRGN
                     LVQNFDLPCQQGKCFSMLLQIPGNQPLPQPSSDEVQWRAPALAEFSDEAADSSERDRL
                     IQAMEQSGWVQAKAARLLNLTPRQIGYALKKHQIPLKHL"
     gene            17552..19054
                     /gene="nifB"
                     /locus_tag="PKI23_05740"
     CDS             17552..19054
                     /gene="nifB"
                     /locus_tag="PKI23_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HNN36475.1"
                     /translation="MQEIAEHKGCGTTGGSGKASCGSSDGPADMAPEVWEKVKNHPCY
                     SEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAAKKVLAVA
                     STIPQMTVLGIAGPGDPLANPDKTFKTFELISKTAPDIKLCLSTNGLALPDHVDTIAN
                     FNVDHVTITVNMVDPEVGQHIYPWIYYKKKRWTGIDAAKILTERQMQGLEMLTARGIL
                     SKINSVMIPGINDQHLVEVNRAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQE
                     LKALQDACEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIDAMEVVYDADTRKAYQ
                     ELVEQERQATVAAKEAEKAELAKLADAEGISLLIAVATKGQGKVNEHFGHASEFQIYE
                     VDAKGSKFVGHRRVDLYCQGGYGEEDSLETVIRAINDCHAVFVAKIGGCPKDDLKKAG
                     IDPVDKYAHQHIEQSVIAYFMDYVGRVRAGEITHQSRGDADIRQGAFIAA"
     gene            19089..19313
                     /locus_tag="PKI23_05745"
     CDS             19089..19313
                     /locus_tag="PKI23_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNN36476.1"
                     /translation="MAYKIVASQCTGCSACEPECPNVAIYEKDGVFVINPKKCTECIG
                     YFDDPQCVAVCPVDNTCVIDGAQPRYQARA"
BASE COUNT         3522 a         6422 c         6209 g         3342 t
ORIGIN      
        1 agcttcgggc tcgatccggt gttcgtgccg gatctgtccg gttcgctgga cggccacgtg
       61 ccggaggact tcagccccac caccatcggc ggcacccggc gcgaggcgat cgccgcgctc
      121 ggttcggcgg gtctggtgct cgccgtcggc gaacagatgc gcacggccgc cgaatggctc
      181 ggccagcaca tcgatgcgcc ggtgcagctg ttcgaccggc tgctgggcct cggcgccacc
      241 gatgcgctga tggcctttct cgcccagcac agcggccggc cggtgccggg aaagtaccgc
      301 cgccagcgca gccagctggt ggacgcgatg ctcgacggcc acttctacac cggcggctgc
      361 aaggtggcga tcggcgccga gcccgacctg ctgcacggcg tgggccagtt cctggccgag
      421 ctcggctgcg agctcgccgc cgccgtcacc accacggact cgccgctgct ggaacgcctg
      481 ccgaccgagg aggtgctgat cggcgatctc gaggacctgg aagcccgcgc cgcgggctgc
      541 gacctgctga tcacccacgc ccacggccgg cagatggcgc agcggctcga cattccattt
      601 ctgcgcatgg ggctgccgat cttcgaccgc ctcggcgccg cgcatcgcct ggcgctcggc
      661 taccgcggca gccgggattt catcttcgag gtggccaacg cgctgatcgc gcacggtcac
      721 gaaccgcatc ccgatgactg gagaacgagc gatgcgcccg aagccggtgc ggcggctgcg
      781 gttgcctaga aacccgaatc accagcgtgc cagcgcccaa ggaggcgtca tgaaaatcgc
      841 gttttgcacc caggatctgc agcgggtcga cgcccacttc ggctgggcca agaacattgc
      901 catctacgag gtgaacccca ccagccacca gttcgtcgag gcgatccagt tcgacggcga
      961 cctgcaggaa gacggcaacg aggacaagct ggcacccaag atcgaggcga tcaaggactg
     1021 cgcgatcctg tatgtggcgg cgatcggcgg ctccggcgcg gcgcgggtgg tggcccacaa
     1081 catccacccg atcaaggtgg cgcagccgga gccgatcgcc gagctgctgg aaaaactgcg
     1141 tgatgtgctg gccggcaccc cgccaccctg gttgcgcaag gtgatgttca agggcgaaag
     1201 ccgtcaattc gatttcgacg acgaggtgca gcatgactga atcccaacca accgcaacgg
     1261 cgacgacgcc gatgcactcc cccttcatca tcgaactggt caagcagtgg cgggcgcagg
     1321 atgcccatgg cgcctgggag ggcaaaagcg atgccgatct gctcgccccc tacctgctga
     1381 ccaaggagca acgccgtcag ctgccgatca tcggtgatcc cgacccggaa accctgtggc
     1441 gggtcgagct gttctacaac gccgtcgggc tcgcgatcga gcgcgccacc ggcgtgatgg
     1501 tgtcgccgat catgaagctg caccacgagg gtttcggccg catggtgctc accgccggcc
     1561 ggctgatcgt cgtcaacaag cagttgcgcg atctgcaccg ctttggcttc gagagtctgg
     1621 aaaagctcgc cgccgaaggc gagaagctgg tcgccgccgg catcggcatg atcggccagt
     1681 atccggcggt ggccaattat ggttgagcct cttcttcagc cctttcattg atgactcaag
     1741 gtgacgcgat gagtgatccg gagcagctca aggccagaat caagaagctc aatgccaagg
     1801 ccaccagcct gaagatgaat ctgcatgacc tgtcggaaga gttgccgatc ggttgggaga
     1861 agattccgct gatcgccgcc gaggcacacg aagcctaccg tgccctggcc gaggcccgca
     1921 gcgaactgga acggatcgga ggctgaaccg atgagtgact tcagcgtcac gctgcccaat
     1981 ggccacgcct ggacgccgcg cttcgtgcag accttgaatg aaacgcgctg catcggttgc
     2041 ggacgctgct atcgggtctg tggccgcgat gtgttcgagc tggtcggcct gaccgaagag
     2101 ggcgaacgga tcgtggtcaa aatgacggcg gccgacgaag acgatgacga cgatgtcgaa
     2161 tacgaacgca aggtgatgac catcgcgcat caggagaact gcgtcggctg cgaagcctgc
     2221 tcacgcatct gtcccaagca gtgtcacagc catgccccgc tgtcactctg actgcatcgg
     2281 cacgcgcccg atcgtcacgg agtcccgcca tgacctccat cgcccacatc acgcctgcac
     2341 cgccatcggc tgagctgctg gccgccgcga cccggccagt ggcgccactg acgctggtct
     2401 atgccggcgt gctggcccgc ttcggcaccc tggccctgcc gatgtcacag ttgcaaccgc
     2461 tgttcgacag ccactttcca cgcgcatcgg ccctgctgcc gcggttcgac ggcgagctgc
     2521 cggcgcagct gcgctgccgg ttcgatgagg tggaggatgt gcgcgacctg ctctatgaac
     2581 accgcagcaa cgacagcaac gacagccgct ggctggccca tgccatggcg gtgggttgcc
     2641 tgggcagtga ccacctgtgg caggatctcg ggctgccgga gcggcgggcg ctgtcgcaac
     2701 tgctgcagga gcacttcacc accctgcatg cccgcaacgg caacaacatg cgctggaaaa
     2761 agttcttcta caagcagctg tgcgagaggg ccgaactcca catctgcaag gcgccgagct
     2821 gcgccgagtg catcgactac ccgctctgtt tttcggccga agcagagtcg gcgtgacctt
     2881 cacccatcca ccctgcgcgc tcgatgatgg cgagctcgac cgcattctcg gcgaggatgt
     2941 cggccatggc gacctgacca ccgcggcact cggcatcggc gcgcggccgg cacgcatcga
     3001 cctgagcgcc cgcgatgcga tggtggtctg cggtgtcgaa gaggcggcac ggctgttcag
     3061 ccgcaatggc tgccaggttg ccacgctgat cgaacgcggc agccgggttg aacgcggcac
     3121 gctgctgttg gcggccgagg gccgggccga tgcgctgcac cgggtctgga agagtgccca
     3181 gaccctgatc gaacatgccg ccggcatcgc gaccgccacc gcgcagatgg tccgcgcggt
     3241 ggcgggcatc ggcaagacgc cggtggtggt ctgcacccgc aagaattttc ctggaacacg
     3301 cgccattgcc gcgctggccg tgcaggccgg tggcgcccag atgcaccgcc tgggtctggg
     3361 cgagacgctg ctgctttttc ctgaacatcg ccagttcatc gcgccagcag cgctggccga
     3421 gaccgtgcat cggctcaggc agcgccatcc ggagaaaaag ctgatcgccg agaccggcga
     3481 cagtgacgag gcggtggcgc tggcgcgggc cggggtcgaa atcctccagc tcgaacgctt
     3541 ctcgccggaa cggctggccg aactgctgcg gaccctgaac catctggccc tgccctgcct
     3601 ggtcgccgcg gctggcggcg tgaccctggc caacgctgcg gcctacgccc gcgccggcgc
     3661 cgacattctg gtgagctcgg cgccctactt cgcggcaccg gccgaggtgc aggtgtgctt
     3721 caccccgatt gaccgatcct cacccggctg aacgtccccc gacgtcggcg caacgacagc
     3781 tcgacgcaga gcgacatcgc cgctgcgtcg agaccacgcc tcagccgaac ttgaacagaa
     3841 tgccgtagcc accgcgcgga tagttccact ccacgttgtg gaattcggtg cagatgatgc
     3901 ggcaggtgcc acactccagg cagccatcgg tgatcagggt gacccggcca tcgccctcca
     3961 gcgtgtagca actggccgga cagcagtggg tgcagggttt ctcttcacac tggctggcgc
     4021 agaccgatgc atcgaggatg ttgatgtggg gccgtccgct gtcgacgcgg tagcggttct
     4081 ggaacagttt ctcttcaaca cggctcattc gaaggccctc cacaatttga atgcatcact
     4141 caccagcccg agccgggagc gtttcatgac gaagttgcgc cgaatggccc gctccttggt
     4201 ccgcttgtcg atgccgtcga cggcaatcag cgtatgggcg gcacggttga tcagctccgg
     4261 ataggtggtg aagaactgct ggtttttctc gacgacatgc ggcagccgcc tgtatttctt
     4321 cagatctttc atcacgaagc tggcgtcgag cttctgccgg tagcgggcca ggttcttctg
     4381 gttcagcggc ttgttctcgc tcttgagctc gatcaccgtc tgtgccgcga gctgcccggt
     4441 ggtcatggcc aggttcgagc cttcgcggtg cacgccgttg acgaacatgc cggaatcacc
     4501 ggcaatcatc cagccgtcgc catagagctt cggcatggcg ttgtagcccc cctcggggat
     4561 cagatgagcg gtgtactcct tcatctcgct gccttcgagc agcggcttga tgccgggatg
     4621 cgccttcatc tcctccagca tggtgtaggg cgagattttc tgccgcttga aatcactgag
     4681 cagacagccg accccgatgc tgatcgactc cttgttggtg tagagaaaac cggtgccaac
     4741 catgccctgg gtgatcttgc ccaccatctc gatcaccacc ccctcgtcgc cactgaggtt
     4801 gaagcggctc tggatggtct cctcgggcag aaagtggatc tccttgacgg ccagcgccac
     4861 atcctgcggc ttgatttcgg gatgaaagcc ggcgcgcttg gcgagcaggg agttgacccc
     4921 gtcggccaga atcacgatgt cggcatagat cgatccaccc aggcggtcgg tcttgacccc
     4981 caccacctgc tggccatcca tgtgcagctc ggtcaccgtg gtctcgcaga tcaccagcgc
     5041 gccggcctcc tgcaccttct tcgaaaacca cttgtcgaac tgggcgcgga tgatggtgta
     5101 gcggttgtag ggcggcgtgt tgaagtcgtt ggagcggtag ctggtgccga cataggcggc
     5161 atcatcgaga atccagccac gctgctcgat gatgtgccgt tccagcggtg cctcatcgcg
     5221 aaaatcgggg atgattctct ccagcgcatc ggcatagagg atggcgccct ggacattctt
     5281 cgaacccggg tattcgccac gctccagttg cagcaccttg agtccggcct tggccagggt
     5341 gtaggcggcg gcattgccgg aaggaccggc accgaccacg atcgcatcga acttctcgtt
     5401 catgggcact ctcctgcact gggttctgtg aacgctaacc ggccatgcgg ctggtcagcg
     5461 cctggcgaaa ggcttcggtc agcgccggca gaatctgcat ggcgttgcca acgattccat
     5521 aggtggcgaa gtcgaagatc ggcgcgttgg gatcctggtt gatcgcgatg atcgcatcgg
     5581 cgccctccat gccgacccgg tgctgcaccg caccggagat gccggcagcg atatagagct
     5641 tgggccgcac ggtcttgccg gactgcccga cctgccggtc ggcgctgacc cacccggcct
     5701 gcaccaccgg ccgggtggca ccgacttcgg cccccagcac ccgcgccaga tcccagatca
     5761 gctcgaaatt ttccggccgc ttcagaccgc gcccgccgga gacgatgatg tcggcaaacg
     5821 gcagggtcga ctgctcgctg ctgtcgtcgg cgatgaactc cagcaccttg gtgacgatgt
     5881 cactctcgat caggctcagc ggatgttcga cgaccggccc gctgcggctg ctgtcgcgct
     5941 cgggcatgcg catcacccgc ggacgcaccg tggccatctg cggccggtag ttgagggtga
     6001 cgatggtgca gagcagactg ccgccaaagg tcggacgggt cgccgccaga ctgcgttttt
     6061 ccgtgtcgat ggcaagaccg gtgcagtcgg cggtcaatcc ggtgcgcagc gtggtggcga
     6121 cactgcctgc cagatcgcgc cccagcgtgc tggcacccag cagcaggatc tccggctggt
     6181 agtggttgac cagatcggtc agcgccctgg tgtagggctg gttgcggtaa tcggccagca
     6241 ccggatcctc caccagatag cagaagtccg cgccatgctg gatcgcctcg gcacagaagc
     6301 cacgcaccgc ctcgccgggg gcgcccagca ccacgccggc cagatcgacc cccaactgat
     6361 ccgccagttt gcgcccctcg ccgagcagtt cgaaggagac cgggtggaca gccccgcgct
     6421 catgctcgat gaagacccac acccccttgt aggcggcaag gcgttcatcc agttcaaatt
     6481 ttccacgacc tgcctttcgg ctctcggcag cggctggagt ggcttgactc atggtctgat
     6541 cctcatgaca gggcgcggcg ggtgatttcg ccgtgcagtt gcggatagcg cgtgaacagc
     6601 ttggaaatca gggtggcggc caggtcgctg ggctgaccgc tctggacgtc gatgatctcg
     6661 gcacgctggg tctgcgcggt gggcgcgaac accttggaga ccaccgtcgg cgagcccttc
     6721 aggccgatca tgttgaggtc ctcgatggca gcagcctcct tgttccacac cttgatctcg
     6781 tggcgggcgg cgcgaaacat gtcgtccatg gtggcgaacc gcagctcgtt gatcccctcg
     6841 agcacggtga ccaggcaggg cagccgggtc tggagccgct gcaccccgcc ctcggcgcgg
     6901 cgctgcacgc tgatctcacg ccgttcgagg tcgagcgact cgatcttgct gacataggtg
     6961 agcagttgca gacccagccg gatggcgatg ccggggccca cctgggcggt gtcgccatcg
     7021 atggtctgct tgccggcaaa cagcagatcg accggcatgt cccgctgaat ctggcgaatc
     7081 gccgcggcca gcgcatagct ggtcgccagg gtgtcggcac cggcaaaggc gcggtcggtg
     7141 agcagaatgg cctcgtcggc ccccaatgag agcgccttgc gcagggcgac ctccgcctgc
     7201 ggcggaccca tcgtcagcac ggtgacacgg gccttgtagc ggtccttgag ccgcagcgcc
     7261 tcctccagcg ccaccatgtc gtaggggttg atgatcgccg gcacgccctg gcgcatgatg
     7321 gtgttggtga ccggatggac gcgaatctgg gcgctgtccg gcacctgctt gatacagacg
     7381 acgatatgca tgtcagacct ctcttgcgcg tgatggcagg gtgcggcgca gtgtgtcgag
     7441 cgacaccgca ccaccttcgg cctcctggaa caccttgaac accttttcgg tggcggcatt
     7501 ggagcggacg aaatcgtcat gggcacgggc cagcagtgcc gcgcaggcct gcttcagcgc
     7561 ggcagcctcc tccagtcggc cgagttcggc cgtggcccgc ttcagatact gctgaaaacg
     7621 catcaggatg tgcagccgat tgacgtgcag cacgccaggg tcataggcga catcgagatg
     7681 gttgaagaac tcctccgccg ccgaaagctg attgagttgg tcgagcagtt gctccaggga
     7741 tgtggtcatg gtcacctcgc tttgatcgcc ttgctgggtc agccctgttg ctcgccgcca
     7801 tcctgacagt cgacggcgca actgccgcac tgcgcatcgc tgccatcggc cgccggattg
     7861 ggcaacagcg cggtttcgag ccggtcgatg cgctccagca gacaggcgag tgccttgcca
     7921 accggatcgg gcatcaggtg gtggtcgaga tcgatgccct gtcgggtgat gcggcggcgg
     7981 tcgatcggca gcaccacccg cccgggaatg ccaaccacgg tggcctcggc cggcacatcc
     8041 tgggtcacca ccgcattggc accgatccgg gcatggtggc cgatggtgat cggcccgaga
     8101 atcttggcgc cggcccccac cagcacgcca tcggccagcg tcggatggcg cttgccaccg
     8161 ctggcggaga ccccgcccag ggtcacgccg tgatagaggg tcacgtcatc gccaatctcg
     8221 gccgtttcac cgattaccac gccgcagcca tggtcgatga agaggcggcg gccgatggtg
     8281 gcgccgggat ggatgtcgat ctgggtcagg gcgcgcgcga cgaagctgag ccagcgggcc
     8341 aggtagcgcc agtgcccaca ccacagccga tgggccaggc gatggatcag cagcgcatgc
     8401 agacccgggt aggtggtcag cacctcgaac ctgccgcggg ccgccggatc gcggctgaag
     8461 acgcagtcga tctcctcgcg cagccgccgg aacagccgcg ggcggggcag tgccgcgtcc
     8521 gacatgccac tcaaccgtgc agagtcacgc atgggctgct ctccagatag aaccgtttca
     8581 gctcgctggg ggtgggcgcc tgtttggttg ccgacgcatg gtcacggacc cgtgacagca
     8641 tctgcccggt ctgccgttcg tccagcacga tgccaagctg ctggtaggcg cgccggaccg
     8701 cgctgccgcc cgagtgcttg ccgagcacga tggtgtgggt gcgccccacc acctccgggt
     8761 cgaaaccctg atagatgtcg gcccgcttga gcagtccatc gacatggatg ccggcctcat
     8821 gggtgaagac cgccgcaccg acgatgctct tgttcgcaga gaccgggcgt ccggaggcca
     8881 gactgaccag ctgcgaaatg gccggaaaag cgcgactgtc gatgccggtc tcgtggccat
     8941 ggatcacccg cagcgccatc accacctctt cgagcggcgc attgccggcc cgctctccca
     9001 acccattgac ggtggtgttg atgtggctgg caccggccag caccgccgcc accgtgttgg
     9061 cggtggccat gccgaggtcg tcatgggcat gcatctcgat gccgatgtcg accacccgca
     9121 ccagccgctg cacatgggtc agggtggcaa aggggtcgag cagtccaagg gtgtcggcga
     9181 agcgaaagcg gctggcaccg gcagcctgag cggcctcggc gacacgcagc agaaagtcgg
     9241 ggtcggcccg cgacgcatcc tcaccaccca ccgacacctc gaaccccaga tcgagcgccc
     9301 gtggtaccag ttgggcaatc tgtgccagca cccagtcccg gtcacgggcg agcttgtgct
     9361 cgatgtgaaa atcggagacc ggaatcgaca ggttgagaat ctgcaccgga catgcggcag
     9421 ctgccgcgat gtcggccgca cacatccgcc cccaggccat cagccgcacc ggcagccgca
     9481 acgcggcgat cgccgccagc gcttcgcgct cttcgcctcc catgatcgga atgcccgcct
     9541 ccagctccgg caccccggcc tcggccagcg cccgggcgat ggccagccgc tcttgcagcg
     9601 tgaagaccac cgacgcggtc tgttcaccat cacgcagggt ggtgtcattg atgatgaccc
     9661 gccgatcgat ggccagtgtg ggttcggaca tgacagatgg tccattgaat gaagagactc
     9721 aacaggggtt cagcaatgac catgccatgc gccgcatctc tgtttttgtc tttttatttc
     9781 atcatgttgc acagatggcg ccatttcgac gaccgacaaa tgtcgtcatt gtcgcctttg
     9841 cgacagtggc tgctgtcgat gatgccgggg ctcttcgaaa gctgtctggc ccggcgccgc
     9901 ttcactgcgg ggaggtggtt acagagggcg aaccgcatga ggaccgggca ggcccggtgt
     9961 gcggaacaac ctggaatctt gcgttgccgt gaatcgctcg ctgcgaacgg cgcctgacgc
    10021 ttcagccgct ttcgtggacg aagttgagcg tataacgcgg gatttcgatc accagatcct
    10081 gctgtcccat gcgtgcctga cagccgaggc gcgagcaggc ttcgagcccc caggccttgc
    10141 cgagctggtc ctcctcttcc tccttcgcca tcggcaggga gtcgaacccc tggcggatga
    10201 tgagatgaca ggtggtacag acgcaggctt tttcgcaggc atggggaagc gccaccccct
    10261 gccgcagcag caggtcgctg agtctggcgc cgggggtggc ctcgaaactg atctgccggg
    10321 gcgagagcgc ttcgtggggc aacagggtga tggtcggcat ggtcactcct cgtcggtggc
    10381 ttcagtcgcc cattcggtcc gggctcagag ctccgccgca agctctgccg cgtgtcgacc
    10441 tgccagcgcc tgatggatgg cggcattcat gcggcgggcg gcgaactcat cggtctcgcg
    10501 ctgcaacagc tcgatgcact gcgtgatccc ggcgcgctcg gccgtggcca gcgccgcttc
    10561 gagccgctgc atctgctgct cgatcagacg ctgctcatcc ggcgagagca gcgcccggtc
    10621 gctgccgagc gcagaccggg ccgcctccag caaccgctct ccttcgagtt tctgctcgcg
    10681 caacatccgc actgcggcat cctcgcgggc atgctcgcgg gagtcgcgca gcatccgcac
    10741 cacctcctgc tcatccaggc cgtaggcggg cttgaccagc acttcggtcg tcaccccgct
    10801 gtgctgctcg caggcggaaa cggtgagcag cccatcggca tcgatctgga aggtgacccg
    10861 aatgcgcgcc gcacccgccg ccatcggcgg aatgccgcgc aactcacagc gcgccagcga
    10921 ccggcagagc tcgacctgtt cccgctcgcc ctgcagcaca tgcagcgtga tggcactctg
    10981 cccatccttg aaggtggtga actcctgggc ccgcgcggtc ggcagggtgc tgttgcgcgg
    11041 aatgatcttc tccaccagac cgcccatggt ctcgatgccg agcgacagtg gcgtcacatc
    11101 gagcagcagc cagccgtcgg tacggttgcc ggccagtgca tcggcctgaa tcgcggcacc
    11161 aagcgccacc acggtatcgg ggtcgaggcg ggtcagcggc tgctggccaa aaaggcgggc
    11221 cacggcctgg cgaatcgccg gcatgcgggt ggcgccgccc accagcacga caccctgaat
    11281 ttcatgggtg ccaacgccgg catcacgcag tgcccgacgc accggcagca gcgttctctc
    11341 caccagtccg gcacagagtt cgtcgaaggt cgcccgctcc agcatgaagc ccagcagcgt
    11401 cccatccgcc agcgtggcat cgatcaccac ccccgccgcg ctgctcagtg cctccttggc
    11461 ttctcgcgcc ttggccagca acagggccga atcaccggca tcgagccggg ccagattgac
    11521 ctgggagagc atccactgat agatgcagag gtcgaaatca tcgccaccca gcgccgaatc
    11581 gccgctggtg gccaacacct cgaacaggcc acggtgcagt cgcagcagcg agaagtcgaa
    11641 ggtgccgccc cccaggtcat agatcgcata gagcccctcc accgactggt cgaggccata
    11701 ggccaccgcg gcggcggtcg gttcgttgag cagccgcagc acatcgatgc cagccagccg
    11761 ggcggcatcc ttggtggcct ggcgctgcgc ctcgtcgaaa taggccggca cggtgatgac
    11821 ggcaccgaac agctcgccgc ccagacgctg ttcggcacgc aaccgcaatg cctgcagcag
    11881 gtcggcggag acctccagcg gcgttttcac accggcggcg gtttcgatgc ggaccatgcc
    11941 ggggcggtcg acgaaccggt agtggcgacg ggccgccatc gacaggtcgt caagctcgcg
    12001 gcccatgaag cgtttgatcg agaccagggt gttgcagggg tcgctgcgct gatgtgcctg
    12061 ggcggcatgg cccaccacac tgctgccatc cgaccgatag tgcaccaccg agggcaacag
    12121 cacacgcccc gcctcatcgc tcagcagttc tcccgcaccg ttgcggacgg tggccaccag
    12181 cgagttggtg gtgcccaggt cgatgccgat cacccagcgc tggctgtgcg gtgcagtgct
    12241 tcgacccggt tcggcgatct gtaacagtgc catgttctac tccagcgcca gtctggcctc
    12301 atcgatcgcg gtgatcagct tgtggatgca gttgagtttg cacaccagct cggcggcgcc
    12361 ggccgcatcg ccctcgacat cgagccgaac ggcaagctgg ccatgcaggg caaagcgctc
    12421 cgcgcagagc agcgtctgca actgtcccag tgccgcccca tcacgctggc gtctggcctg
    12481 gccgagctgt tcgtgccaat ccatctgctg cagcaggaaa ttcgccggca gcacggcgga
    12541 ttcgctcagt gccaccatgc cgtgacgcag ccgcagcagg tagcaggcgc gctcgagcgg
    12601 gttcctgagg gtctgatagg cctcattggc gcgggtggcc cactgcaggg cgagacgctg
    12661 ctcggtgacg ctgccaccgg catggcggtc gggatgcacc tggctctgaa gctggcgata
    12721 ggccgccgcc agccggacgc tgtcgagctg gaaccgcacc ggcagaccga agagcgcgaa
    12781 gtgatcgtca gccaccgcgc tgcgctgtgc cgcgccctgc tgcgtcaggg cgttcatagc
    12841 gtgaagctct cgccgcaacc gcagtggttg gcggcattgg ggttgttgaa ggcaaagcct
    12901 tcgttgagcc cctcacggac gaaatcgagt tcggtgccct cgatgtagac cagactgccc
    12961 gggtccacca gcagggtgat gccacggctc tcgaagcaca ggtcatcggg actggccaga
    13021 tcgacgaatt cgagcagata ggacattccg gagcagccgc tggggcgaac gccgatgcgc
    13081 aggccgatgc ctgcaccacg ctggtcgagg aatctcctca cctgcctggc ggcgctttcg
    13141 gtcagggtga tcgacatggc gtaacctcgc actctcaggt ggcgaacgac ttgccgcaac
    13201 cacagctggt cttggcattg gggttcttga tcacgaaacg tgacccatcg agcccatcgt
    13261 gaaagtcgat ctcggccccg accagatagg agagactcag cggatcgatc agcagctgca
    13321 caccatgccg atcgaatgcg gtgtcctctt catcggcctg gtcatcgaag ctgaaaccat
    13381 actgaaaacc ggagcatccg ccaccggaga ccgtgacccg cagcttcagc tccgggcagc
    13441 cctcctcggc aatcatctcc ctgaccttgc tggcggcacg ctcggtgaac atcaactgcg
    13501 ccgcgggcga tgccagcgtt ggatcgacga cttctgtctg catgggggtg actcctagga
    13561 ggcggccgta agccgggtga tctcggtggc cccggccgtt tcgacgccgg caggccgttt
    13621 gctctggtaa tcctcgatcg ccgccttgat cgcatcctcg gccagcaccg agcagtgcac
    13681 cttcaccggt ggcagcgcca gttcatcggc aatctcgcgg tttttgatct gcatcgcctc
    13741 gctcagtgtc cgccccttga gccactcggt caccagcgaa ctggaggcga tggcactgcc
    13801 gcagccgtag gtcttgaagc gcgcatcctc gatgatgccg acctcgttga ccttgatctg
    13861 caaccgcatc acgtcaccgc aggccggcgc gcccaccatg ccggtgccga cactggcatc
    13921 ctcctggtcg agaacgccga cattgcgcgg gttctcgtag tggtccatca acttgtcgct
    13981 gtacgccatc gttctgactc ctgaaaaaga gggggattag ggtcaatgcg ctgcccagct
    14041 cacgctgttc aggtcgatgc cctgctggtg catgtcccac agcggcgaca gcgcgcgcag
    14101 ccgctccaca cccgcccgga ccgtgaccag cgcatggtcg atctccgcgg cagtggtgaa
    14161 gcgtccgagc gagaaacgga tcgagctgtg cgccagctca tcgctgagcc ccagggcgcg
    14221 cagcacatag gagggttcga ggctggccga ggtgcaggcc gagccactgg agaccgccag
    14281 ctccttgatc gccatcatca gcgactcccc ttcgatgaaa ttgaagctga cgttgagcgt
    14341 gtgcggcacc cgctgctgcg gatcaccatt gagatggacc tcgccgatct gcatcagccc
    14401 cgccaacatg cggtcacgca gcgcggtgat gcgggcactg tcggccgcca tctcctgttt
    14461 cgccaggcga aaggcttcac ccatgccgac gatctgatgg gtggccagcg tgcccgagcg
    14521 catgccgcgc tcgtggccac cgccatgcat ctgcgcctcg atgcgcaccc gcggcttgcg
    14581 ccggacgtaa agcgcgccga tccccttggg gccgtagctc ttgtgggccg agaacgacat
    14641 cagatcgacc ggcagttcgg ccaggtcgat cccgaccttg ccggtggact gggcggcgtc
    14701 gacatggaac agcacgccat gctgacgaca gatcgcgccc agactggcga tgtcctgaac
    14761 gacgccgatc tcgttgttga cgtgcatcac cgacgccagc gtggtgtcgg gtcggatcgc
    14821 ctcggcgaac tgctgcggat cgatcagacc attcgattgc ggagcgagat aggtcacctc
    14881 gaagccgaac cgctccagtt cgcgaaaact gtcgagaacc gccttgtgtt cggtcttcac
    14941 cgtcagcagg tgtcgccccc ggttctgatg aaaatgggca gcgcccttga tggccaggtt
    15001 gtccgactcg gtggcgcccg aggtccagac gatctcgcgc ggttcacagt tgaccagcgc
    15061 cgcgacctcg gcgcgcgccg cttcgatcgc ctcctcggcc tgccagccga ggcggtgcga
    15121 acggctcgcc gcattgccga aatttccggt cagaaagggc agcatcttcg ccaccacccg
    15181 cggatcgatc ggcgtggtgg cagcgtagtc gagatagatc ggctgcttcg aagaggtcgg
    15241 cgcgttcggt gatctcactg attccatccc ctctgccctc aacggattca ctcaatgcat
    15301 ggcgtggtca atcctggcaa tgccgttttc gtgccatctc ataaaaacaa ttattttcaa
    15361 taggttgtcg aaacatgacc ccgaaaccgc aatgtcgctg acccgacact cctgtcagct
    15421 ttggaacaac gaatcctcca tgaatcaggc cagacgaagg gcgccgttgc atttgcttgc
    15481 gcggcaactg gcgcgccgca cgctgagtgc ccgacgcagc agagcgcctt ttcgagaagt
    15541 ggagcgcaat ttgctgcatc caacccgcag gcaaagcagt tcaacagatg gcatgcccgg
    15601 agtgactcaa ggtggtgaca gcaagcaatg gcggcaagcc gatgggcaac gttgccagaa
    15661 gcaggcatga ggcggtggcc agccaactgc tggccatcta cgaaatcagc aaggtgctcg
    15721 gctcctccct cgacaccgaa aagagcctgc gcggggtgct gaacattctg agcgcccacc
    15781 tgcggatgcg ccgtggcatg gtctgtctgc gccaaccctc cggcggtttt tacatcgccg
    15841 gcgcgcccga tctgaccagt caggagatcg aacggggcaa ataccgcagc ggcgaggggg
    15901 tgatcggcca gattatcaaa agcggcatgc cgatcgtcgt gcccgacatc cgcctggagc
    15961 cacgctttct caaccgcacc gaatcacgcg acttttccgc cgatcaggtg atcgcctttc
    16021 tgggtgtgcc gatccaggcc ggcaatgaac tgctcggcgc ccttgcggtc gaccgcgaaa
    16081 cggaagatgc cctcagcacc acccatttcg aacgggacat ccgctttctg aagatggtgg
    16141 ccaatctggt cggccagatg atccggctgc atcgcaacat cgccgccgaa cgcgaagagc
    16201 tgctggtcga gaagagtcgc ctgcagaagg agctgcgcgg caagtacagc ctcgacagcg
    16261 tgattggcca gagcaggcgg atgcaggagg tgttcgccga ggtgcatcag gtggcgccga
    16321 gtcgggccac ggtgctgctg cgcggcgaga gtggcaccgg caaggaggtg atcgcccggg
    16381 cgatccacta tctgagcccg cgcaaggaca aacccttcat ccgcgtcaac tgcgccgcac
    16441 tgaccgagtc gctgctcgaa tcggagctgt tcggccatga gaaaggcgcc ttcaccggcg
    16501 ccaccagcga ccgcaagggg cggtttgaac tggccgatgg cggcacgctg tttctcgatg
    16561 agatcggcga ggtctccccg gcctttcaga gcaagctgct gcgggtgctg caggagcgcg
    16621 agttcgaacg ggtcggtggc aaccggccga tccatgtcga tgtgcggatc atcacggcca
    16681 ccaaccggaa tctggaagag gcggtatcga acaaggagtt ccgggccgac ctctactacc
    16741 gcatcaatgt ggtcacactc gcgctgccgg ccctgcgcga gcggcgcgaa gacattccgc
    16801 tgctggcggc attctttctg gacaagttca accgcgaaaa ccagcgcaac ttgaccttct
    16861 cggccgaagc ggtacggacc ctgctcgcct gcaactggcc cggcaatgtg cgggagctgg
    16921 agaactgcat cgagcgcacc gccaccatga cccgtggcaa cctggtgcaa aacttcgacc
    16981 tgccgtgcca gcagggcaaa tgcttctcga tgctgttgca gattcccggc aaccagcctc
    17041 tgccgcagcc atcgagcgac gaggtgcagt ggcgcgcgcc cgcgcttgcg gagttcagtg
    17101 acgaggccgc agacagctcc gagcgtgacc ggctgattca ggccatggag cagtccggtt
    17161 gggtccaggc caaggcggca cggctgctca acctcacgcc acgccagatc ggctacgcct
    17221 tgaagaagca tcagattcca ctcaagcatc tgtagcgccc cgccgcttca tcccggtgcg
    17281 atcagccgcc tcgctccgcc tgtcggctct gcaacaaaaa cgacaccgac accgattgtg
    17341 tctgattgcc gacaatcgcc tcctctacgt gaatttttat acttaacaat gggttggatt
    17401 cagctctcat cccggcacgc tgtttgcgta gagggtttga cagccaaatt cttcatccag
    17461 acagacttgc aagccattgg agcggaacat ggatcagagc aacacgttga gccccatcag
    17521 cctctcgtct ccagcaccac tggagcagat catgcaggag atcgccgagc acaagggctg
    17581 cggcaccacc ggcggcagcg gcaaggcgag ctgcggctcg tccgacgggc cggccgacat
    17641 ggcgcccgag gtgtgggaga aggtgaagaa ccacccctgc tacagcgagg aggcgcatca
    17701 ccactacgcg cgcatgcacg tggcggtggc gccggcctgc aacatccagt gcaactactg
    17761 caatcgcaag tacgactgcg ccaatgaatc ccgtcccggc gtggtgagcg agaagctcac
    17821 gccggaacag gcggccaaga aggtgctggc ggtggcctcg accatcccgc agatgacggt
    17881 gctcggcatc gccggaccgg gcgatccgct cgccaatccc gacaagacct tcaagacctt
    17941 cgagctgatc tcgaagaccg cgccggacat caagctgtgc ctgtcgacca acggtctggc
    18001 gctaccggac cacgtcgaca ccatcgccaa cttcaacgtg gaccacgtca ccatcaccgt
    18061 gaacatggtc gaccccgagg tcggccagca catctacccg tggatctact acaagaaaaa
    18121 gcgctggacc ggcatcgacg ccgcgaagat cctgaccgag cggcagatgc aggggctgga
    18181 gatgctgacc gcgcgcggca tcctcagcaa gatcaactcg gtgatgatcc ccggcatcaa
    18241 cgaccagcac ctggtcgagg tgaaccgggc cgtcaagagc cgcggcgcct tcctgcacaa
    18301 catcatgccg ctgatctcgg cgcccgagca cggcaccgtg ttcggcctga acggccagcg
    18361 cggcccgacc gcgcaggagc tgaaggccct gcaggacgcc tgcgagggcg agatgaacat
    18421 gatgcggcac tgccgccagt gccgcgccga cgccgtcggc ctgctcggcg aggaccgcag
    18481 cgccgagttc accaccgaca agatcgacgc catggaggtg gtctacgacg cggacacgcg
    18541 caaggcctac caggagctgg tcgagcagga gcgccaggcg acggtcgcgg ccaaggaggc
    18601 ggaaaaggcc gagctggcga agctggctga cgccgaaggc atcagcctgc tgatcgccgt
    18661 cgccaccaag ggccagggca aggtcaacga gcacttcggc cacgccagcg agttccagat
    18721 ctacgaggtc gacgccaagg gctcgaagtt cgtcggccac cgccgcgtgg acctctactg
    18781 ccagggcggc tacggcgagg aggactcgct cgaaaccgtg atccgcgcca tcaacgactg
    18841 ccacgccgtg ttcgtggcca agatcggggg ctgcccgaag gacgacctca agaaggccgg
    18901 catcgacccg gtggacaagt acgcccacca gcacatcgag cagtcggtga tcgcctactt
    18961 catggactac gtcggccgcg tgcgcgccgg cgagatcacc caccagagcc gcggcgatgc
    19021 cgacatccgc cagggtgcct tcatcgccgc ctgagcctct tttttcatca tccagcagga
    19081 gatctttcat ggcttacaag attgtcgcat cgcaatgcac cggctgttcg gcctgtgaac
    19141 ccgagtgccc caatgtcgcg atctatgaaa aggatggggt ctttgtcatc aaccccaaaa
    19201 agtgcaccga atgcatcggc tacttcgatg atccgcagtg tgtggcggtc tgtccggtgg
    19261 acaacacctg cgtcatcgac ggcgcccagc cgcgctacca ggccagagca tgaggtgacc
    19321 cgtgaacatc accctgacgg cagcagccga aaaattcatg cagcgcatgg tccgcttcgg
    19381 cggcgagggc gtgaccggct ttcggttgca ggtgagcccg ggcggctgct cgggcgtcac
    19441 cagtgagttc agtgtggagc agtcaccccg gcagggtgat gccgtgctgg agtgc
//
LOCUS       DAORCQ010000038        19415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1664_length_19415_cov_6.405888, whole genome shotgun sequence.
ACCESSION   DAORCQ010000038 DAORCQ010000000
VERSION     DAORCQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 19415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19415
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1664_length_19415_cov_6.405888"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..679
                     /locus_tag="PKI23_05750"
     CDS             56..679
                     /locus_tag="PKI23_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36477.1"
                     /translation="MNEHWLLQADETLTKLVANILQVSHKDSSRLASALIAKTSGAAG
                     AAGVLGLIGAFGTASTGTAIAGLSGAAASNATLFWLGSLVGGGVFAGTILTGGIGLVI
                     GYLGLKFWKGKPRPLESMTDEEKAIVDASLGLVKAFREQRESKVVVKKSEARFVLEKA
                     WRPLIKRFADYKDHRARKTLNLKNLIELGNRRRELERLTKEFESCTS"
     gene            667..1389
                     /locus_tag="PKI23_05755"
     CDS             667..1389
                     /locus_tag="PKI23_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36478.1"
                     /translation="MHVLKAGTSSAAVTVATVLANLMNKAHLHFSSSEQLVVDALRRS
                     NKDLADMPLSEMGGYLRNMDDDSLRGLASNVKGIYHEFQFIRRENLDGDAVTARIFPE
                     TNHPGADVVLSRDGKDFAELQLKATDSSSLLVKHFEKYPDIPVAATDEVAKSIPGVKS
                     SGFSDAELEGDVTSAFSSVSDQAHISQLQDATAISGLLAAAMNAGDVLSGKATTKQAS
                     KTTLKDMGVAVSSTFLVDLLFS"
     gene            complement(1695..2204)
                     /locus_tag="PKI23_05760"
     CDS             complement(1695..2204)
                     /locus_tag="PKI23_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36479.1"
                     /translation="MSDPETRLDRLNLRIRNNPVVASLIILGSIVIALSTFTNAAKNL
                     LDLVMTETRPNINGEWKAEITYDWINAKYSETFTFNGDGEEVHGTVSFLGAKRGILEG
                     KVSEGKLRFIAKTRESLGDWSNTKEVVHRYHGKISDDEIRFVMQSEGGFSEHVPIEFT
                     AHRVPGTSR"
     gene            2942..3916
                     /locus_tag="PKI23_05765"
     CDS             2942..3916
                     /locus_tag="PKI23_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HNN36480.1"
                     /translation="MKTRNLLAALVAAACTTSAHAQITSINLGGYTLNGSYALDTLND
                     MGLEASAVTYARDRGSLFFVGDEGLGVVEISRSGQTLGTMAFDWTGTGSSHNDAEGLA
                     YLGNGELVVVDERPQIAYRFTYAKDGTVALNNTPSVAITGSTASVGNVGTEGISVDPR
                     NGQFYTVKQDTPAQLRVSTLNFLVGGGSATTSNLFSGSADLFGLNNLSDVQTLSPIDA
                     LIGHSAASNLLILSLDSRKIVEIDSTGTVLSFFDLTALTTQAIEGVTVDELGRIYLVA
                     EDSGTPNSRLFVLTPPAPVPVPAAVWMFGSALVGVAGFVRRRRPHAGA"
     gene            complement(4100..4792)
                     /locus_tag="PKI23_05770"
     CDS             complement(4100..4792)
                     /locus_tag="PKI23_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36481.1"
                     /translation="MQRYAATLLAASFGMLFASTTQAALVVTEVAPQTVAGTSSAING
                     DWWELTNFGAVGVNLLGYQWADTEDALPGTDSNLFPSFVINPGESIIILEEAASSKDA
                     WRTNWGIAPSVRILALDEMVDDSTPEGDTFSGLGSADDGVFFYGPNGDLLSSYTYAST
                     VRGTSFEQSGAGVNLGLSLVGENGALLAANNNIGSPGVAVAPVPVPGLGIGFGIATTA
                     LLSCSRRRRIAT"
     gene            complement(5133..6524)
                     /locus_tag="PKI23_05775"
     CDS             complement(5133..6524)
                     /locus_tag="PKI23_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HNN36482.1"
                     /translation="MKRFFSFAMLWLLCLSGGSTLAFAAEPLLTLEASELGSVAVHGQ
                     KGQTQQLAILFSSEEGLADVDLQAIDDLVAQGLTVAVIDTRLALQRLQGFNQDCLYLP
                     GPLEWISHSVQQQLGFTHYSKPLLLGRGEGGVLVYASLVQAPALAFAGGVSVDVASFR
                     LGFGKPLCNLDRNRLEPHDQRFDPGYALGGWWQVGTTTALKNGVGQFVKAADEASQNL
                     VTLLEQPAPLSRSYGEAAQQVLKSPSQSELAQTLEDLPLVEVSSQSTTRTLVIIYSGD
                     GGWRDLDQSLGELLKQKDLAVLGIDALRYFWSKRTAEESALDLARILIHYRKAWQIEN
                     VVLVGYSFGADILPAIYNHLPAPLQSSVRLISLLVPAFKADFSIQVSGWFGADASDEA
                     MPLAPEIARIDRTKLQCFYGTDEADESLCLDPVMQGSEIIARPGGHHFDENYDIIAEQ
                     ISSAIQRRLAAKR"
     gene            complement(6521..9160)
                     /gene="mprF"
                     /locus_tag="PKI23_05780"
     CDS             complement(6521..9160)
                     /gene="mprF"
                     /locus_tag="PKI23_05780"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HNN36483.1"
                     /translation="MNRKEITTSDNAVGQIDPPRRRPPLVPPLLSRLKPHLFSLISLG
                     LFIAALWAIHHMLAGITSAHLLEEIGSLNRQQIGLALFFALASFVALMGYDWSALNYI
                     GQRLPASTVAFASFCGYAFSNTLGLSLLSGGSVRYRIYVVKGLDAADVGRVTLFNMLA
                     FGIGIHIVGAAALTVRPELLVTLSGVSAPWLRAMGAAVLIAVALLLSWAHGRTAPVRL
                     ARWSFRLPKTRLTLFQLAVSVVDVLCCGACLYVLIPSSDLHFVEFMVVYALAVMAGIT
                     SHVPGGLGVFEGIMLLALSGKVSAEGLGAGLLMYRIIYHLGPLLLAMLLLALKELRDR
                     ASPTWSAVLDHLQSLGGRSVPYLASVMVFASGLVLLLSSATPATPERLALIRVFVALP
                     LVEISHLLSSVVGLGLLIVAHGLYRKLNGAYLLALLLCLSGALFSLIKGIDYEEGLVL
                     FATALTLLLCRHEFYRHTTLLDSPFTTTSLMAILGAVGAMVWMIFFSYQHVEYANHLW
                     WKFAYGADASRSLRSAFGVTVTLFALGLYWLLRPPRQPAQHPSAEALEQAEAIIQQQD
                     NTTAQLALMGDKRLLFTETCDAFLMYGIRGASWIALGDPVGSMDTAEELAWRLREMAD
                     SAGGRIAFYQARPALLPIYLDMGLTPMKLGEEGVVLLDRFSLEGSSNKHLRYACNRGE
                     RDGLVMEVIDKAQVPQMLPDLEEISTAWLTSRNTEEKGFSLGAFIPDYICRNGVAIVR
                     QQGRPIAFATLMTTATHAEASLDLMRHRPDAPSLTMEFLFIRLMQHFKQQGFCRFNLG
                     MAPLSGLEAHPLAPLWYRFGDLIYNRGARFYNFQGLRQFKEKFDPIWEPRYLVSEGGL
                     SALLAITDTAALIAGSYKGIVMK"
     gene            complement(9209..10027)
                     /locus_tag="PKI23_05785"
     CDS             complement(9209..10027)
                     /locus_tag="PKI23_05785"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNN36484.1"
                     /translation="MSDILSGLLLGIVEGLTEFLPVSSTGHLIIVGDLLDFNDERGKL
                     FEVVIQLGAILAVCWEYRQRLTSTLGQLHRDPQAQRFVLNLLLAFLPAALLGFLFIKQ
                     IKLYLFNPLSVALALVVGGVFILWTERRALPSHIDTVDELKPLDAFKVGCAQMLALVP
                     GVSRAGATIIGGLWFGLSRKAATEFSFFLAIPVMFAATAYDLYKHGAALQFSDWPLFA
                     TGFLCAFFSAFVAVRGLLRYVSNHDFTLFAWYRIGFGALILLTAQLGWVDWSSG"
     gene            complement(10095..10487)
                     /locus_tag="PKI23_05790"
     CDS             complement(10095..10487)
                     /locus_tag="PKI23_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNN36485.1"
                     /translation="MYSLLKMTHLLMVALTIGLFLVRARWMLFDPQRLQTRWVKVLPH
                     IIDTLLLASAIGLCVVIGQYPLIDGWLTAKFLALLLYIVLGTIALKRGKTRTIRTAAL
                     VASLVTLGYLLWVARSHQPFPWLALAAS"
     gene            10669..10818
                     /locus_tag="PKI23_05795"
     CDS             10669..10818
                     /locus_tag="PKI23_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36486.1"
                     /translation="MKKLSRFLLLLTLFSNIALADCYKDGKAYPTGTVIDGFICTADG
                     AWVKA"
     gene            10850..11821
                     /locus_tag="PKI23_05800"
     CDS             10850..11821
                     /locus_tag="PKI23_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36487.1"
                     /translation="MEAKTQSEKSGSSRLTLLIDVISKLVPAAAVVVAAIVANQFQAA
                     NSAANLLNQREQADSALRAGMLRDLINPITASGKDLGKMPLDQEQLLVELLALNFNEH
                     FELKPLMLHVDGRLAREQSAPNGQQPSIDARESLRSVARRVTQRQLAQLTAIGDHAPA
                     EQKSCIYTYRLKLAPAQRAAQTAVAPPTPACTDQPINQFFGDLISLESPDGAYSLAFT
                     LRPPKNWQEQTFDALMYISGRNSTGQQVKRVSAEQDFTLTWFDFPFTDNTLLADGVRF
                     SLVLDRVDASRNEVQVKLVWFPRDYFSPRERPVNHLQQREKLGLLPK"
     gene            12013..12963
                     /gene="fmt"
                     /locus_tag="PKI23_05805"
     CDS             12013..12963
                     /gene="fmt"
                     /locus_tag="PKI23_05805"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNN36488.1"
                     /translation="MRILFAGTPEFAAVSLRALLASRHSVVGVLTQPDRPAGRGRKPQ
                     PSAVKQVALSAGIALRQPETLRDSVVVADLAQFEADLMVVVAYGLLLPEPVLGLPRLG
                     CLNVHGSLLPRWRGAAPIQRAILAGDQQSGVTIMQMERGLDTGPMLLRRSCPIQADDS
                     SASLSQRLADLGAETLLAALDLLESGDARFEPQDEASANYAAKIGKQEAEIDWRNPAT
                     SIDRQVRAFNPAPIAFTHLDEIRLNVWAGRPLEHSADALPGTLLGHSEQGLEVACGQG
                     RYAITQLQWPGGRPLASGEIWNARRQQLAAGRQLRLPLSS"
     gene            12960..14267
                     /gene="rsmB"
                     /locus_tag="PKI23_05810"
     CDS             12960..14267
                     /gene="rsmB"
                     /locus_tag="PKI23_05810"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNN36489.1"
                     /translation="MKGAQSPRLLAALAVAGVVQQGQSLGTVLPPLEARCAPRDRALL
                     LELSYGTLRWYPRLDRLLGLLLDRPLKPRQAKLKALLLVGFHQLLNLRIPPHAAIHET
                     VAAVTGLGEPHAKGLVNGVLRNLDRHRDRLLQQLDDDPVAATAHPAWLLALLREAWGD
                     ASDHLLAANNGHPPFTLRVNLQRISRAQCLQRLVEAGLEGVPGTLADSAITLQSACPV
                     SQLPGFEQGLVSVQDEAAQLAAILLDPQPGERLLDACAAPGGKSAALLERQPGLAELV
                     AIDIDAARLQQVRDTLQRCALDATLMAADALDLASWWDGRPFDRILLDAPCSATGVIR
                     RHPDIKLLRRESDIAPLAARQRALLEALWPTLASGGRLLYATCSVLPAENAAVVADFL
                     VDHADARTAPLPITGSVASAIGCQLLPTPGGPDGFYYALLEKR"
     gene            complement(14274..14855)
                     /locus_tag="PKI23_05815"
     CDS             complement(14274..14855)
                     /locus_tag="PKI23_05815"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNN36490.1"
                     /translation="MNRTEATPQRCPWCGDDPLYLHYHDHEWGVPLHDDQRLFELLTL
                     EGAQAGLSWITVLRKRERYRHCFAGFDPQAVARFGPTEVEALLADPGLIRNRRKIESA
                     IHNAGRVLTLQARHGSLAAFLWGMVDGVPQVNRFRTAAEVPATTPLSDRMAKALKQAG
                     FSFVGSTICYAMMQSIGMVNDHLVDCFRHPDHQ"
     gene            14928..15881
                     /gene="glyQ"
                     /locus_tag="PKI23_05820"
     CDS             14928..15881
                     /gene="glyQ"
                     /locus_tag="PKI23_05820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNN36491.1"
                     /translation="MQADVSTFQGLILALQHYWAAQGCVVMQPTDLEVGAGTFHWATF
                     LRAIGPESWNTAYVQPCRRPTDGRYGENPNRLQHYYQFQVILKPSPLDIQERYLDSLR
                     MLGIDPTLHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCHPVSG
                     EITYGLERIAMYLQGVDSIFDLVWARGPQGVVTYGDVFHQNEVEMSAYNFEQANTEVL
                     FRHFAEHEAACQRLLDANLPLPAHEMVLKASHTFNLLDARRAISVTERQQYILRVRTL
                     ARGVAERYFHSRLALGFPLAPEPLRQEILALHRAEQEPHHG"
     gene            15874..17955
                     /gene="glyS"
                     /locus_tag="PKI23_05825"
     CDS             15874..17955
                     /gene="glyS"
                     /locus_tag="PKI23_05825"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNN36492.1"
                     /translation="MAEADFLLEIGTEELPPKALPALMQALADLVAAGLRQAALSHGQ
                     IEPFATPRRLAVRVRALATRQPDRIEERRGPALSAAFDASGAATPAALGFARSAGVSV
                     EQLERLETDKGVWLVQRRQIEGAATAELLPDLLNQALAQLPVPKKMRWGSSRVEFVRP
                     VHWVVMLLGDQVVPGTLLGVEAGNLSHGHRFHAPGAIALSHPEHYPALLRQQGWVIVD
                     FAERRERIRQLVEAEAARIGARAEIDPLLLDEVTALVEWPVALLGNFDPLFLDVPMEV
                     LISSMKAHQKYFHLVDRSGRLLPHFITLSNLQSSDPAQVIAGNERVIRPRLADARFFY
                     DNDRKQPLDGWRAPQREIIFHNQLGTLHDKSERLARLAAIIAGTLGADAALARRAGEL
                     AKCDLLSAMVGEFPELQGTMGRYYARHSGEPEVVAIALEEQYRPRFAGDALPTTPVGS
                     ALALAEKLDTLVGLFGIGQPPTGTRDPFALRRAALGTLRILVEGRHDLDLPALLEAAR
                     DGYPTLPQHASVVPELYRYLLERFRAWYQDEGIGSDVISAVLARPNANPHDIHLRLLA
                     VNHFVTLESAAALASANKRVANLLNKQEGQTEGAIDSALLVDAAEQQLAAAMATKSAA
                     LTPLLTARDYSQALTLLAGLRPQIDAFFDQVMVMVEDEALRNNRLRLLAALRALFVQI
                     ADISELRSGGK"
     gene            17952..18503
                     /gene="gmhB"
                     /locus_tag="PKI23_05830"
     CDS             17952..18503
                     /gene="gmhB"
                     /locus_tag="PKI23_05830"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNN36493.1"
                     /translation="MRPKLVILDRDGVINHDSDSYVKSADEWQPIAGSIEAIGRLCRH
                     GYQVAVASNQSGLGRGLFDQAALDAMHAKMRRLIAAEGGTLAAIRYCAHGPDAGCDCR
                     KPAPGLLLEIGRLLGLPLDGVPFIGDTLKDVEAARAVNCRPMLVRTGKGERTLMQHPE
                     LAATLPVFADLAAAVDDLLNTPA"
     gene            18515..19258
                     /locus_tag="PKI23_05835"
     CDS             18515..19258
                     /locus_tag="PKI23_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNN36494.1"
                     /translation="MRRPPTWLTALRSGLFYLGYSLFTVLFCGTALLFVPLLPPLTRF
                     YYLRNWNRVILCWLRICCGIKTQVEGLENIPPAPFVVLSKHQSPWDTIYLQLPFGPLS
                     TVLKRELLSIPFFGWALALIRPIPIDRDKPREALRQVMEAGKERLAAGVSILVFPEGT
                     RIEPGQQGKYAKSGASIACSMGVPVLPVALNAGLCWPAHGFLKYPGTIRVVIGPALPS
                     SDVSSAQLTEQAREWIESTVERISRVEGV"
BASE COUNT         3681 a         6159 c         6077 g         3498 t
ORIGIN      
        1 atgcgacagg tagggatgcg acatttacgc ttcgttgggc ctcaatgggg atatcatgaa
       61 cgaacactgg ttgcttcaag cagatgagac actgaccaag ctcgttgcga atattctgca
      121 ggtgtcgcac aaggattcat caagactcgc cagcgcattg atagcaaaaa cctccggcgc
      181 ggctggcgcg gcgggagtac tgggtctgat cggcgcattt ggcacagcat ctacaggtac
      241 agctatagcg ggcctctccg gggctgcggc gtcaaatgcc actctgttct ggttgggctc
      301 gcttgtcggc ggaggagtct ttgctggcac cattcttacg ggcggcattg gactggttat
      361 cggatacttg ggcctgaagt tctggaaagg caagccacga cctttggagt ccatgacaga
      421 tgaagaaaaa gctatcgttg acgccagcct tggacttgtc aaagcgttca gagaacaacg
      481 ggagtcaaag gtggtggtga agaaatctga ggctcgtttt gtgctcgaaa aagcatggag
      541 gccgcttata aagcgattcg cggactacaa agatcatagg gcaagaaaaa cattgaacct
      601 caagaatctt atagaactcg gcaatcgtag acgagagttg gaacgtctca ctaaggagtt
      661 tgagtcatgc acgtcctaaa agcgggaaca tcatcggcgg ctgtaactgt cgcaacggtt
      721 cttgccaatc taatgaacaa ggcgcatctc cacttttcat ctagtgagca gcttgtagtt
      781 gacgcgctgc gtcgctccaa taaggacttg gctgatatgc ccttatctga aatgggaggc
      841 tatttgcgaa acatggacga tgactcgttg cgcgggttgg caagcaacgt aaaaggcatt
      901 taccacgagt tccaattcat ccgtcgcgaa aacctggatg gcgatgcagt tactgccaga
      961 atatttccag agacaaacca tcccggtgca gatgtggtgc tgagccgcga tggcaaagac
     1021 tttgcggagc ttcagctaaa agcaacagat agcagttcat tgttggtaaa gcacttcgag
     1081 aagtatcccg acattcctgt tgcggccact gatgaagtag caaaatcaat tcctggagtt
     1141 aaaagctctg gcttcagtga tgcggagctg gaaggcgatg ttacaagtgc tttttccagt
     1201 gtttctgatc aagctcatat ctcacaactg caagatgcaa cggcaatctc cggtctactc
     1261 gccgcagcca tgaatgctgg cgatgtactt tctggcaagg caacaaccaa gcaagcatct
     1321 aagaccacat tgaaagatat gggggtagca gtctcatcca cgtttttggt tgatctgctt
     1381 ttctcgtgag cgtcgtaatc cggcccaaca aggtgctcca gcagtattga ggacctcaga
     1441 cgcggcgtga tcagaatttg gggacacgcg taggatggct ccatgaagcc atttcgctgg
     1501 aacgccgaga agaacgaaac gcttacgtct gaaggggtat ttcgttcgag agcatcgtgg
     1561 ttgccatcga atccggcggg ttactggaca tcctggccca cccgaaccag tcgaaatatc
     1621 caacacgaat tggggacaga ccacggttat ggcacattcc ccgacaaaac ccagtcatgc
     1681 gcgcaaggtg gcggctatcg cgaggtgcca ggcactctgt gggcggtgaa ctcgatgggt
     1741 acgtgctcag aaaaaccacc ttcggattgc atgacaaacc ggatttcatc gtccgagatc
     1801 tttccatgat aacggtgcac cacctctttg gtgttgctcc agtctccaag actctcgcgt
     1861 gttttcgcaa tgaatcggag tttgccttcg ctcaccttgc cttcgagaat tcctcttttc
     1921 gcacccagaa atgaaaccgt tccatggacc tcttcgccgt cgccattgaa ggtgaatgtt
     1981 tctgaatact ttgcattgat ccagtcataa gtgatctcag ccttccattc accattgatg
     2041 ttgggccgag tttcagtcat gaccagatca agaaggtttt ttgccgcatt ggtgaaggtc
     2101 gacagggcaa tgacaatcga tcccaggatg atcagcgaag cgacgaccgg attgttcctg
     2161 atccgcaaat tgagtctgtc gagtcgtgtt tctggatcgc tcatggcgat gacccttcct
     2221 gtttgacgat cgcggtgccg ccactcgtca tggatcgacc gatttcccca tgagcagact
     2281 ttaacatttt ctgaaaaaaa tggactcgat ctctttctgt ttccatcatg aatggtgctc
     2341 aaacacagtg cgccatgacc gcctgcggca aaggcccgct gggccggagc tggatgcgct
     2401 ccagctggtg ctgtttgagc aggtcgatgc gggctgtaat cctggccgag agagtgatgg
     2461 ctggcgatgc aagtgatcgc tcacattcac ggaaacctct gcctcagcag cggtagggtg
     2521 ggcaaagcgc agcatgccca ccaaaacgcg gaagatcact gagacagtgg ctactgagaa
     2581 accacgtggg cacgctgcgc tttgcccacc ctaccgctgg ctgacccgtg cactgctcgt
     2641 cacgacttac ggcagcacgc cctacaccga cgagcaggtc acggcggcca ggctcgccgg
     2701 agatctctgc ggaatcatct tgcgagatct cgtcatcgtc agccagttcc gtgtggcgcc
     2761 ggtaccggtg ccaggcgccg tttgcatgcc ccgtcgtcca ttcgcggatg catgggctgc
     2821 tgcggattca tcgtgctgaa acagtcgatt cgtagtctgc cgcgatacct cgtctgttgc
     2881 gcgcgctgcg cgacaggccg ggggcgacac cgtccgtttc acccgcatct ggagaaccgc
     2941 catgaagacc cgcaatctgc tcgctgcgct cgttgccgcc gcctgcacga cttccgcaca
     3001 cgcgcagatc acgtcgatca atctcggcgg ctacacgctg aacggcagct atgcgctgga
     3061 cacgctgaac gacatgggcc tcgaggcctc tgccgtcact tacgcgcgtg accgcggcag
     3121 cctgttcttt gtcggcgacg aagggctcgg ggtcgtcgaa atttcgcgca gtggtcagac
     3181 cttggggacc atggcgttcg actggaccgg caccggatcg agccacaacg atgccgaagg
     3241 tctcgcttac ctcggcaacg gtgagctggt cgtcgtcgat gagcggccac aaatcgcgta
     3301 caggttcacc tacgcgaaag atggcactgt cgcgctcaat aacaccccca gcgtggccat
     3361 caccggcagc accgcgagcg taggcaatgt cggtaccgaa ggtatcagtg tcgatcctcg
     3421 caacggacag ttctacacgg tcaagcagga cactccggcg caacttcgtg tcagcacact
     3481 gaattttctg gtgggcggtg gctctgcgac gaccagcaac ctgttctcgg gttctgctga
     3541 cctgttcgga ctgaacaacc tctccgatgt ccagactctc tctcccatcg atgcactcat
     3601 cggccattcc gcggcaagca acctgctcat cctcagtctg gattcccgca agatcgtcga
     3661 gatcgacagt accggcacgg tgctgagctt cttcgatctg acggcgctta cgacgcaggc
     3721 catcgaaggc gtgacggtcg atgagctcgg tcgcatttat ctggtggccg aggattcagg
     3781 tacgccgaat tcccgcctgt tcgtgctcac tcctccggcg ccggttcccg ttccggcggc
     3841 cgtgtggatg ttcggtagcg cgctggttgg tgtggccggt ttcgtgcgcc gccgccggcc
     3901 acatgccggc gcctgatggc gcctggtccc gacgggatcg gccatcgtct agatggccga
     3961 atcgacagcg acaggctggt gcggaggctg tagccggaga ccgaccacgc cgcaggttgt
     4021 gaagacaaaa aagaagggtc ggcagcatag gctgccgacc cttcttttcg ttcctccccc
     4081 gaattcccga cttcaggact caggtggcga tgcgacggcg acggctgcag gagagcagcg
     4141 cggtcgtggc tatcccgaaa ccgatgccga gtcctggcac cggcacaggc gcgacagcga
     4201 cgccgggcga cccaatattg ttgtttgcgg ccaggagggc cccgttttca ccgaccaggc
     4261 tgagtccgag attcactcct gcaccgcttt gctcgaatga ggtgccgcgc acggtcgatg
     4321 cgtaggtata gctgctcaac aggtcgccgt tcggaccgta aaagaatacg ccgtcgtcag
     4381 cgctgcccag ccccgaaaag gtgtcgccct ccggtgtcga gtcgtcgacc atttcatcga
     4441 gtgcgagaat ccgcaccgaa ggcgcaatgc cccaattggt tcgccacgca tctttgctcg
     4501 atgcggcttc ttccagaatg atgatcgact caccgggatt gatcacgaaa gagggaaaaa
     4561 ggttgctgtc agtgccgggc agcgcgtctt cggtatcggc ccactggtag cccagcagat
     4621 tgacgccgac ggcgccgaag ttggtcagtt cccaccagtc tccattgatt gcagacgagg
     4681 tgcccgcgac ggtctgcggc gcgacctcgg tcacgaccag cgctgcctgg gtggtggaag
     4741 cgaacaacat gccgaaagaa gcggcgagca gggtggctgc gtagcgttgc atcgaaaatc
     4801 tcccggttat ttttgccatg agtgagtgcc agaaacgcag caacgttgtc acaggtttgt
     4861 gtcagcctgg tgaagcagcc gttttcattt ggggcagtca tccgcaagga agaggcgcag
     4921 cctgccggcg aagcgccatc ggggttcaga acgccggtca cgcgtttgcg caatggcccg
     4981 gcggtttctg cacgggtcgg gccgctgaag catgggcctc ggcgggggca cgccgagcgc
     5041 ctggtgcggc tggcggtggt tgtagaaccg gatccaatcg cgatcacgcg caggatgtgc
     5101 ttgaggaaaa ccgtggtccc caacgctcct cttcagcgct ttgctgccag ccggcgctga
     5161 atggcgctgc tgatctgttc ggcgatgatg tcgtagtttt catcgaagtg gtggccgccg
     5221 gggcgggcga tgatttcgct gccttgcatg acggggtcga ggcagagtga ttcgtcggct
     5281 tcgtcggtgc cgtagaagca ttgcaatttg gtgcgatcga tgcgggcgat ctcgggcgcc
     5341 agcggcatgg cttcgtcgct ggcgtcggcg ccgaaccagc cgctgacctg gatgctgaag
     5401 tcggccttga aggccggtac cagcagcgag atcagccgca ccgaggactg caacggcgcc
     5461 ggcaggtggt tgtagatggc cggcaggatg tcggcgccaa aggagtatcc gaccagcacc
     5521 acgttctcga tctgccacgc cttgcggtag tggatgagga tgcgcgccag gtcgagggcg
     5581 ctctcttcgg ccgtccgttt cgaccagaag tagcgcagcg catcgatccc cagcaccgcc
     5641 agatccttct gcttgagcag ttcgccgagc gattggtcga gatcgcgcca gccgccgtca
     5701 ccggagtaga tgatcaccag tgttctggtc gtcgattgcg acgagacttc gaccagcggc
     5761 aggtcctcca gcgtctgcgc cagttcgctc tggctggggc tcttcagcac ttgttgggcg
     5821 gcttcgccat agctgcgcga caagggggct ggctgctcca gcagggtgac caggttctgg
     5881 ctggcctcat cggcagcctt gacgaactgg ccgaccccgt tcttgagtgc ggtggtcgtg
     5941 ccgacctgcc accatcctcc cagcgcataa cccggatcga accgttggtc atgtggttcg
     6001 agacggttgc gatcgaggtt gcagagcggc ttgccgaaac cgagcctgaa tgatgcgaca
     6061 tcgacactga caccgccggc gaaggccagc gccggcgcct gcaccagact ggcgtagacc
     6121 agcacgcccc cctcgccacg gcccagcagc aacggcttgc tgtaatgggt gaagcccagc
     6181 tgctgctgga cggagtggct gatccactcc agtgggcccg gcaggtagag gcaatcctga
     6241 ttgaagccct gtagccgttg cagcgccagc cgggtgtcga tcactgccac ggtcagcccc
     6301 tgtgccacca ggtcatcgat cgcctgcagg tcgacatcgg ccaggccctc ttcggatgaa
     6361 aagagaatcg ccaactgctg cgtctgcccc ttctgaccat ggaccgcgac gctgcccagt
     6421 tcgctggctt cgagtgtcag cagcggttcg gctgcgaagg caagggttga tccgccgctc
     6481 aggcagagca gccagagcat ggcaaaggaa aagaaccgct tcacttcatg acgatccctt
     6541 tgtaactgcc ggcaatcaac gcggcggtgt cggtgatcgc cagcaacgcg ctgaggccgc
     6601 cttctgaaac caggtagcgc ggttcccaga tcggatcgaa cttctccttg aactggcgca
     6661 gaccctgaaa gttgtagaag cgcgcccccc ggttgtaaat cagatcgcca aagcggtacc
     6721 agagcggggc cagggggtgt gcttccagcc ccgacagcgg cgccatgccc agattgaagc
     6781 gacaaaaacc ctgctgcttg aagtgctgca tcagccggat gaacaggaac tccatcgtca
     6841 aggacggtgc atcgggtcgg tggcgcatca ggtcgagcga cgcctcggca tgggtggcgg
     6901 tggtcatcag ggtggcaaag gcgatcggtc gaccctgttg acgaacgatg gcgacaccgt
     6961 tgcggcagat gtagtcggga atgaatgccc ccagcgaaaa acctttctct tcggtgttgc
     7021 gggaggtgag ccaggcggtg gagatctctt cgaggtctgg cagcatctgc ggcacctgcg
     7081 ccttgtcgat cacctccatg accaggccat cgcgttcgcc gcggttgcag gcatagcgca
     7141 gatgcttgtt gctggagccc tcgagcgaaa agcgatcgag cagcaccacg ccctcctcgc
     7201 ccagcttcat cggcgtcagc cccatgtcga ggtagatcgg cagcagggcg ggccgcgcct
     7261 ggtagaatgc gatccggccc ccggcgctgt cggccatttc acgcagtcgc caggccagct
     7321 cttcggccgt atccatcgat ccgaccggat cacccagcgc aatccagctt gcgccacgga
     7381 tgccatacat caagaaggca tcacaggttt cggtgaacag cagccgcttg tcgcccatca
     7441 gggccagctg ggcggtggtg ttgtcctgct gctgaatgat cgcttcggcc tgctccagtg
     7501 cttcggccga ggggtgttgg gcgggctgcc tgggtgggcg cagcagccag tagaggccga
     7561 gcgcgaacag cgtgaccgtg acgccgaagg ccgagcgcag cgaacgggag gcatcggcgc
     7621 cgtaggcaaa cttccaccag aggtggttgg catattcgac atgctggtaa ctgaagaaga
     7681 tcatccacac catggcacca acagccccca gaatcgccat cagcgaggtg gtggtgaagg
     7741 gcgagtcgag cagtgtggtg tggcggtaga actcgtgtcg gcacagcagc agcgtcagcg
     7801 cggtggcgaa gagcaccagc ccctcttcat agtcgatgcc cttgatcagc gaaaacagcg
     7861 ccccgctcag acagagcagc agggccaaca gataggcgcc attgagcttg cggtacagtc
     7921 catgcgcgac gatcagcaac ccgagaccga cgaccgaaga gagcaggtgg gagatctcga
     7981 ccagcggcag tgcgacaaac accctgatca gcgccagccg ctcgggggtt gctggcgtgg
     8041 ccgacgagag cagcagcacc agaccgctgg cgaagaccat caccgaagcc agatagggca
     8101 cagaccggcc acccagcgac tgcagatggt cgagcacggc actccaggtc ggcgacgccc
     8161 ggtcacggag ttccttgagc gccagcagca gcatcgccag caacagcggg cccagatggt
     8221 agatgatgcg gtacatcagc aggcccgcac ccaatccctc ggcggagacc ttgcccgaga
     8281 gcgccaacag catgatgccc tcgaacaccc caagcccgcc cggcacatgg ctggtgatgc
     8341 cggccatgac cgccaacgca tagaccacca tgaattcgac gaagtgcagg tcgctcgacg
     8401 gaatcaagac atagaggcag gcgccgcaac agagcacatc cacgacggaa acggccagtt
     8461 gaaacagggt gagccgcgtc ttcggcagtc gaaacgacca gcgtgccagc cgtaccggtg
     8521 cggtgcgccc atgggcccat gacagcagca gggccaccgc aatgagcact gcggcaccca
     8581 tcgcgcgcag ccagggtgcc gaaacgccag agagcgtcac cagcaactct ggccgcaccg
     8641 tcagcgccgc ggcaccgacg atgtggatgc caatgccgaa ggcgagcatg ttgaacaggg
     8701 tcacccgccc gacatccgcc gcatccagcc ccttgaccac atagatgcgg tagcgcaccg
     8761 aaccacccga cagcaggctc agacccaggg tgttggagaa ggcatagccg cagaatgagg
     8821 caaaggcgac ggttgaagcc ggcaggcgct ggccaatgta gttgagcgcc gaccagtcat
     8881 agcccatcag cgccacgaag ctggccaggg caaagaacag tgccagacca atctgctgcc
     8941 ggttgaggct gccgatctcc tccagcagat gggcggaggt gatgcctgcc agcatgtggt
     9001 ggatggccca cagggccgcg atgaagagtc ccagcgagat cagcgagaag aggtgcggct
     9061 tcaggcgcga cagcagcggg ggcaccagcg gtggccgcct ccggggcgga tcgatctgac
     9121 cgactgcgtt gtctgaagtc gtgatctctt tacgattcat cacaccaaca gcggcatcag
     9181 agtgaagggt tcggttgcat cacccatctc agccactcga ccagtcgacc cagcccagtt
     9241 gcgccgtcag caggatcaat gccccgaaac cgatgcgata ccaggcgaag agggtgaaat
     9301 catggttgga gacatagcgc agcaggccac gcacggcgac gaaggcgctg aagaaggcgc
     9361 agagaaagcc ggtggcaaac agcggccagt cgctgaactg cagggcagcg ccatgtttgt
     9421 agaggtcgta ggcggtggcg gcaaacatca ccggaatcgc cagaaagaat gaaaattcgg
     9481 tggcggcctt gcgtgacagt ccaaaccaga gtccgccgat gatggtggcc ccggcgcggg
     9541 agacgcctgg caccagcgcc agcatctgcg cgcagccgac cttgaaggca tccagcggtt
     9601 tcagttcatc gacagtatcg atgtgcgatg gcaaggcgcg ccgctcggtc cagagaatga
     9661 agacgccacc gaccaccagc gccagcgcca ccgacagcgg attgaacaga tagagcttga
     9721 tctgcttgat gaacagaaag ccgagcagcg cggccggcag aaaggccagc agcaggttca
     9781 gcacgaagcg ctgcgcttgc ggatcgcggt gcagttgacc gagggtcgag gtgagccgct
     9841 ggcggtactc ccagcagacc gcgaggatgg ccccgagctg gatcaccacc tcgaacagct
     9901 tgccgcgctc gtcattgaag tcgagcaggt cgccgacgat gatcaggtga ccggtgctcg
     9961 acaccggcag gaattcggtc agcccctcga cgatgccgag cagcagcccg ctcaggatgt
    10021 cactcatgct tcacctgcgt tgaataagga aggatgaaaa gggccggtcg aatggccatc
    10081 cggttccggc agcctcagga ggctgccagc gccaaccatg gaaagggctg atggctgcgg
    10141 gccacccaga gcagatagcc gagcgtcacc agtgatgcca ccagcgccgc cgtgcggatc
    10201 gtccgggtct tgccgcgctt cagggcgatg gtgcccagca cgatgtagag cagcagggca
    10261 aggaatttgg cggtcagcca gccatcgatc agcggatatt ggccgatcac cacacagagg
    10321 ccaatggcgc tggccagcag cagggtgtcg atgatgtggg gaagcacctt cacccagcgg
    10381 gtctgcaacc gctgcggatc gaacagcatc cagcgagcac gcaccagaaa gagtccaatg
    10441 gtcagtgcca ccatcaacag gtgggtcatc ttcagcaggc tgtacatcgt tcgacctcgc
    10501 aggataaagc cggggattgt agtgaccgcc aagcctgacc gccagcggtg acaattgtgc
    10561 tgccgtgctt gtgcccggtt cgataaagtg gttcgattga tccgaccgtc agcccacggc
    10621 cacaccggca gcggtggcgg cactcttcga ccatcaggag aagcccaggt gaagaaactt
    10681 tcccgctttc tgctgctgct gaccctcttc agcaacatcg cgctggccga ctgctacaag
    10741 gatggcaagg cctacccgac cggcaccgtg atcgacggct tcatctgtac cgccgacggc
    10801 gcctgggtca aggcctgagt ccagacgccc gcacccaagg cgcagccgca tggaggccaa
    10861 gacgcaatca gagaaaagcg gcagcagcag gctcacgctg ctgatcgatg tcatcagcaa
    10921 gctggtaccg gcggccgccg tggtggtcgc cgccatcgtt gccaaccagt ttcaggccgc
    10981 caacagcgct gccaacctgc tcaaccagcg cgagcaggcc gacagtgccc tgcgtgccgg
    11041 catgttgcgt gacctgatca atccgatcac cgcctcgggc aaggatctcg gcaagatgcc
    11101 gctcgatcag gagcaactgc tggtcgaact gctggcactc aacttcaacg agcatttcga
    11161 actgaagccg ctgatgctgc atgtcgacgg ccgtctggcg cgcgagcagt ccgcaccgaa
    11221 cgggcagcaa ccgagcatcg atgcacgcga atcgctgcgc tcggtggccc gccgcgtcac
    11281 ccagcggcag ttggcgcaac tgaccgccat tggtgaccat gccccggccg agcagaagag
    11341 ctgcatctac acctatcggt tgaaactggc cccggcgcag cgggccgccc agactgccgt
    11401 cgcgccgccc acgccagcct gcaccgacca gccgatcaat cagtttttcg gcgatctgat
    11461 cagccttgaa tcacccgatg gcgcctacag cctcgccttc actcttcgac caccgaaaaa
    11521 ctggcaggag cagaccttcg atgccctgat gtacatctcc ggccgaaaca gcacgggtca
    11581 gcaggtcaag cgtgtttcgg ccgaacagga tttcaccctc acctggttcg attttccctt
    11641 taccgataac accttgctgg ccgatggcgt gcgtttttcg ctggtgctcg accgcgtcga
    11701 cgccagccgc aacgaggttc aggtcaagct ggtctggttc ccgcgtgact acttctcgcc
    11761 gcgcgaacgc cccgtcaacc acttgcagca gcgcgaaaag ctgggattgc tgcccaaata
    11821 accaccctgc ggtctgtcgg caccgtcgcg ctgccttttg tagcggaatc ggctaagctg
    11881 ccccacccct gcgtctgaag cgcgtcacct tttgtcgatg ccagacagtc ggttggctgc
    11941 ggttcttgac gctgaactgc gtcattgcct gtcggccggg atcgacgcct gcccaatcca
    12001 gagagctccg ttttgcgcat cctgttcgcc ggaacccccg aatttgccgc cgtcagcctg
    12061 cgggcgctgc ttgccagtcg ccattcggtc gttggcgttc tcactcagcc cgatcggccc
    12121 gcaggccgtg gccgcaagcc gcagccgagc gcggtcaagc aggtggcgct cagcgccggc
    12181 atcgcgctgc gccagcccga gacgctgcgc gattcggtgg tcgtcgccga tctggcccag
    12241 ttcgaggccg atctgatggt ggtggtcgcc tatggcctgc tgctgccgga gccggtgctc
    12301 gggctgccgc gcctcggttg cctcaatgtg catggctcgc tgctgccccg ctggcgcggc
    12361 gccgcgccga tccagcgtgc cattctggct ggcgatcagc aaagtggcgt caccatcatg
    12421 cagatggagc gcggcctcga caccggcccg atgctgctgc ggcggagctg tccgatccag
    12481 gccgacgaca gcagcgccag cctgagccag cggctggccg acctcggtgc cgagacactg
    12541 ctggccgccc tcgatctgct ggagtccggt gacgcacggt tcgagcctca ggacgaagca
    12601 agtgccaact acgctgccaa gatcggcaag caggaggccg agatcgactg gcgcaacccg
    12661 gccacgtcga tcgaccgcca ggtgcgcgcc ttcaaccctg cgccgatcgc cttcacccat
    12721 ctggatgaga ttcgcctcaa tgtctgggcc ggccggccgc tggagcattc ggccgatgca
    12781 ctgcccggca cgctgctcgg ccacagtgaa caggggctgg aggtggcctg cggccagggc
    12841 cgctatgcca tcacccagtt gcagtggcct ggtgggcggc cgctcgccag tggcgagatc
    12901 tggaacgccc gccgccagca acttgctgcg ggacggcagt tgcgcctgcc gctgtcgtca
    12961 tgaagggcgc acagtcgccc cgcctgcttg ccgcgctggc ggtcgccggc gtggtgcagc
    13021 aggggcagtc gctcggcacc gtgttgccac cactggaggc ccgctgcgcg ccccgcgacc
    13081 gggcgctgct gctcgaattg agctatggca cgctgcgctg gtacccccgc ctcgaccggc
    13141 tgctcggtct gctgctcgac cggccgctga agccgcgcca ggccaagttg aaggcgctgc
    13201 tgctggtcgg ttttcatcag ctgctcaacc tgcgcattcc gccccatgcg gcgattcacg
    13261 agacggtggc ggccgtcact ggcctgggtg agcctcacgc caaggggctg gtgaacggcg
    13321 tgctgcgcaa cctcgaccgt caccgcgatc gattgctgca gcaactcgat gacgatccgg
    13381 tggccgccac ggcccatccg gcctggctgc tggcgctgct gcgcgaagcg tggggcgatg
    13441 caagtgatca cttacttgcc gccaacaatg gccatccccc cttcacattg cgggtcaacc
    13501 tgcaacgcat cagccgtgcg cagtgcctgc agcgactggt cgaagcgggt cttgaagggg
    13561 tgcccggcac gctggccgac agcgccatca cgctgcaaag cgcctgtccg gtgagccagt
    13621 tgccgggctt cgagcagggg ctggtcagcg tgcaggatga ggcggcgcaa ctggccgcca
    13681 tcctgctcga cccgcaacca ggtgagcgct tacttgacgc ctgtgcggcg cctggcggta
    13741 aaagtgccgc gctgctggag cggcaaccgg ggttggcgga gctggtggcg atcgacatcg
    13801 acgcggcacg gttgcagcag gtgcgcgaca ccctgcaacg ctgcgcgctg gatgccacgc
    13861 tgatggctgc cgatgcactc gatctggcca gctggtggga tggccgcccc ttcgaccgca
    13921 tcctgctcga tgcgccctgt tcggccaccg gcgtgatccg tcgccatccc gacatcaagc
    13981 tgctgcggcg tgagagtgac attgcgccac tggccgcccg gcagcgggca ctgctcgaag
    14041 cgctctggcc cacgctggcc agcggcggcc ggctgctcta tgccacctgt tcggtgttgc
    14101 cggccgaaaa cgcagcggtg gtcgcggatt ttctggtcga ccatgccgat gcgcggacag
    14161 cgccgctgcc gatcaccggt tcggtcgcca gcgccatcgg ttgccagttg ctgccgacgc
    14221 cgggtggtcc cgatggcttc tactacgccc tgctggaaaa gcgctgagcc ggttcattgg
    14281 tggtcgggat ggcgaaaaca gtcgaccagg tggtcgttca ccatgccgat cgactgcatc
    14341 atggcatagc agatggtgct gccgacgaag ctgaagccgg cctgcttcag cgccttggcc
    14401 atccggtccg acaatggcgt cgtcgccggc acctcggccg cagtgcgaaa gcggttgacc
    14461 tgcggcacgc catcgaccat cccccacagg aaggccgcca ggctgccatg tcgcgcctgc
    14521 agcgtcagca ccctgccggc gttgtggatg gccgattcga tcttgcgccg gttgcggatc
    14581 aggcccggat cagccagcag cgcctccacc tcggtcggac cgaaccgggc caccgcctgc
    14641 ggatcgaagc cggcgaagca gtggcggtaa cgctcgcgct tgcgcagcac ggtgatccag
    14701 gagagtccgg cctgcgcccc ctccagcgtc agcagttcga acagccgctg gtcatcatgc
    14761 agcggcaccc cccattcgtg gtcgtggtag tgcagataga gtggatcatc gccgcaccag
    14821 gggcagcgtt gcggggtcgc ctcggtccga ttcatgtcac tccttcaggg cgagcgggta
    14881 taattcctca ctttatcagg acataggaca ctctcttggt caatgccatt caagctgatg
    14941 tctccacctt tcaggggttg atcctcgccc tgcagcacta ctgggccgcc caaggctgcg
    15001 tggtgatgca gcccaccgat ctggaagtgg gcgccggcac tttccactgg gccacctttt
    15061 tgcgcgccat cggcccggag agctggaaca ccgcctatgt gcagccgtgc cgacgcccga
    15121 ccgatggccg ctatggcgag aaccccaacc gcctgcagca ctactaccag tttcaggtga
    15181 ttctgaagcc ctcgccgctc gacattcagg agcgctatct cgactccctg cggatgctcg
    15241 gcatcgaccc caccctgcat gacatccgtt tcgtcgagga caactgggag tctcccaccc
    15301 tcggcgcatg gggtctgggc tgggaggtgt ggctgaacgg catggaggtg acccagttca
    15361 cctacttcca gcaggtcggc ggactcgact gccatccggt cagcggcgag atcacctatg
    15421 gcctggagcg catcgccatg tacctgcagg gggtcgacag catcttcgac ctggtctggg
    15481 cccgcggccc gcagggggtg gtcacctatg gcgatgtctt tcaccagaac gaggtggaga
    15541 tgtcggccta caacttcgag caggccaaca ccgaggtgct gttccgtcac tttgccgagc
    15601 acgaggcggc ctgccagcgg ctgctcgacg ccaacctgcc actgccggcg cacgagatgg
    15661 tgctgaaggc ctcgcacacc ttcaacctgc tcgatgcccg ccgcgccatc tcggtgaccg
    15721 aacggcagca gtacatcctg cgcgtgcgca ccctggcccg cggcgtggcc gagcgctact
    15781 tccacagtcg cctggcgctc ggttttccgc tggcgcccga accactgcgg caggagattc
    15841 tggccctgca ccgcgccgaa caggagccac accatggctg aggccgattt tctgctggag
    15901 attggcaccg aagagctgcc acccaaggcg ttgccggccc tgatgcaggc gctggccgat
    15961 ctggttgccg ccggattgcg ccaggcagcc ctctcccacg gccagatcga acccttcgcc
    16021 accccccgtc ggctggcggt gcgggtgcgt gcgctcgcca cccgccagcc cgaccgcatc
    16081 gaggagcggc gcggcccggc gctctcggcc gccttcgacg ccagcggcgc agccaccccg
    16141 gcggcacttg gctttgcccg ttcggccggc gtgtccgtcg aacaactgga gcggctcgaa
    16201 accgacaagg gggtctggct ggtgcagcgt cggcagatcg agggggccgc cactgccgag
    16261 ctgctgcccg atctgctcaa ccaggcgctg gcacaactgc cggtgccgaa gaagatgcgc
    16321 tggggcagca gccgggtcga attcgtccgg ccggtccact gggtggtgat gctgctcggt
    16381 gaccaggtgg tcccgggcac cctgctcggc gtcgaagcgg gcaacctcag ccatggtcac
    16441 cgttttcatg caccgggcgc catcgcgctg tcccatcccg agcactatcc cgccctgctg
    16501 cggcagcagg gatgggtgat cgtcgatttt gccgagcgac gcgagcggat tcgccaactg
    16561 gtcgaggccg aggccgcccg catcggcgcc cgcgccgaga tcgacccgct gctgctcgac
    16621 gaggtgaccg ccctggtcga gtggccggtg gcgctgctgg gcaacttcga tccgctcttt
    16681 ctcgatgtgc cgatggaggt gctgatctcc tcgatgaagg cgcaccagaa atatttccat
    16741 ctggtcgatc gcagtggtcg actgctgccg cacttcatca ccctcagcaa cctgcaaagc
    16801 agtgatccgg cccaggtaat cgccggcaac gagcgggtga tccggccgcg cctggccgat
    16861 gcccgcttct tctacgacaa cgaccgcaag cagccgctcg atggctggcg tgcgccgcag
    16921 cgcgagatca tcttccacaa tcagctcggc accctgcatg acaagagcga gcggctcgcc
    16981 cggttggctg cgatcatcgc cggcacgctg ggcgccgatg cggccctggc gcgtcgcgcc
    17041 ggtgaactgg ccaaatgcga cctgctgagc gcgatggtgg gcgagttccc cgaactgcaa
    17101 ggcaccatgg gccgctacta tgcccgccac agcggcgaac ccgaggtggt ggccatcgcc
    17161 ctcgaggagc agtatcgccc ccgttttgcc ggcgatgcgc tgcccaccac cccggttggc
    17221 agcgcactgg cgctggccga aaagctcgat accctggtcg ggcttttcgg catcggccag
    17281 ccgcccaccg gcacccgcga ccccttcgcg ctgcgccgtg ccgccctcgg cacgctgcgc
    17341 atcctggtcg aaggccgcca tgacctcgac ctgccggcac tgctcgaagc ggcccgcgat
    17401 ggctatccga cactgccgca gcacgccagc gtcgtgcccg agctctaccg ttacctgctg
    17461 gagcggttcc gcgcctggta tcaggatgaa ggcatcggca gcgatgtgat cagcgcagtg
    17521 ctggcgcggc ccaatgccaa tccgcacgac atccatctgc ggctgctggc ggtcaaccac
    17581 ttcgtcaccc tggagtcggc ggccgcgctg gcttccgcca acaagcgggt cgccaacctg
    17641 ctcaacaaac aggaaggtca gaccgaaggg gccatcgaca gcgcactgct ggtcgatgcc
    17701 gccgaacagc aactggctgc ggcaatggcg accaagtcgg ccgcgctcac cccgctgctg
    17761 acggcacgcg actacagcca ggcgctgaca ctgctggccg gcctgcgtcc gcagatcgat
    17821 gcctttttcg atcaggtgat ggtgatggtc gaggatgaag cgctgcgcaa caaccgactg
    17881 cggctgttgg cggcgctgcg cgcgctcttc gtgcagatcg ccgacatctc cgaactgcgc
    17941 agcggcggca aatgagaccg aagctggtca ttctcgatcg cgacggcgtc atcaaccatg
    18001 actccgacag ctatgtgaag tccgccgacg agtggcagcc gattgccggc agcatcgagg
    18061 ccatcggccg cctctgccgg catggctacc aggtggcggt ggccagcaac cagtctggcc
    18121 tcggtcgtgg cctgttcgat caggcggcac tcgatgcaat gcacgccaag atgcgccggt
    18181 tgatcgcggc cgagggcggc acgctggcgg ccatccgtta ctgcgcccac ggtcccgatg
    18241 ccggttgcga ctgccgcaag ccggcgcccg gtctgctgct ggagattggc cggctgctcg
    18301 gcctcccgct cgacggcgtc cccttcatcg gcgacacgct caaggatgtc gaggccgccc
    18361 gcgcggtcaa ctgccggccg atgctggtgc gcaccggcaa gggcgagcgc acgctgatgc
    18421 aacatcccga actggcggcc acgctgccgg tgttcgccga cctggccgcc gcggtcgacg
    18481 acctgctcaa cacccctgcc tgaggagagc gcccatgcgc cgtccaccga cctggctcac
    18541 cgcactgcgc agtgggctct tttacctcgg ctacagcctc ttcaccgtgc tcttttgcgg
    18601 cactgcgctg ctgttcgtcc cgctgctgcc gccgctgacc cgcttctact acctgcgcaa
    18661 ctggaaccgg gtgatcctct gctggctgcg catctgctgc ggcatcaaaa cccaggtgga
    18721 ggggttggag aacatccctc cagcgccctt cgtggtgctc agcaagcacc agagcccatg
    18781 ggacaccatc tacctgcaac tgccgttcgg cccgctctcc accgtgctca agcgtgaact
    18841 gctgtcgatt cccttcttcg gctgggcgct ggcgctgatc cgcccgatcc cgatcgaccg
    18901 cgacaagccg cgcgaggcgc tgcgtcaggt gatggaagcg ggcaaggagc ggctcgccgc
    18961 cggggtgtcg attctggtct ttcccgaggg cacccgcatc gagcccggtc agcagggcaa
    19021 atatgccaag agcggcgcca gcatcgcctg cagcatgggg gtgccagtgc tgccggtggc
    19081 gctcaacgcc ggcctctgct ggccggcgca tggctttttg aaataccccg ggaccatccg
    19141 ggtggtcatt ggcccggcgc tgccgagcag cgatgtcagc agcgctcaac tgaccgaaca
    19201 ggcccgcgag tggatcgagt cgacggtcga gcggatcagc cgcgtcgagg gcgtgtgatg
    19261 cctacgcgcg ccagaccgag cccgaacagt gcgagcatgg agggtttggc cgctgcgttg
    19321 gggtcgggct gctgcggcaa ctgattttga aaaattgggg agatgtttag ctctggctag
    19381 cagaaaaccg gaaaaccgtg gtctgtcccc aattc
//
LOCUS       DAORCQ010000039        18905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1743_length_18905_cov_5.403660, whole genome shotgun sequence.
ACCESSION   DAORCQ010000039 DAORCQ010000000
VERSION     DAORCQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18905
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_18905_cov_5.403660"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKI23_05840"
     CDS             complement(<1..801)
                     /locus_tag="PKI23_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNN36495.1"
                     /translation="MRASLPPFEHVRALVVGDLMLDRYWHGDTQRVSPEAPVPVVRVT
                     RIEERAGGAGNVARNLAALGVSVELAGVVGVDEMGERLTQILVDAGVRCHFQHSAAHP
                     TITKLRVISRNQQLLRLDFEERFDPMPALSSQALHEALKRSQVLLLSDYAKGTLADPQ
                     PLIQAARAVAMPVLVDPKGSEFDRYRGATLLTPNQAEFEQVAGRCADEAELIGKARAM
                     TARLDLTALLVTRGGQGMLLVPREGDPINLRAQAREGYDVTGAGDTVIA"
     gene            900..1520
                     /locus_tag="PKI23_05845"
     CDS             900..1520
                     /locus_tag="PKI23_05845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNN36496.1"
                     /translation="MEQVELPIDIDQVKGFLHPDEGAALYRQALIASRLGPCLEIGSY
                     CGKSTLYLAAACRQTDALLYAIDHHGGSEEHQLGEEYHDPELYDREEAVMDSFRTFRR
                     NLRRAGLDAQVVPIVAPSALAARHWNTPLGMVFIDGGHSLEAALTDYRCWAGHLVPGG
                     ILAIHDIFPDPRDGGQAPHQIYQLASASGLFEQLPMARTLGLLRRL"
     gene            complement(1467..2546)
                     /locus_tag="PKI23_05850"
     CDS             complement(1467..2546)
                     /locus_tag="PKI23_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36497.1"
                     /translation="MTQLYLSKGALPLDYFRPSIDYILDVQRSDGAIPWFHGGHFDPW
                     DHTEAAMGLSIAGHLEEAEQAYDWLRQWQLEDGSWWARYEQGQPVLDAEARKESNFTA
                     YVATGVWHHYLITTDESFLRRMWRTVERAIDHVVALQCRRGTIPWAIDAEGVPRAQAL
                     VTGCSSVHKSLECALNIATVLGHARPQWSLARERLGDALRNHPERFDQSKTRYSMDWF
                     YPILGGVVRGAAARQRLQTRWSSFVEHELGCRCVSDEPWVTVAESCELTLALLAAGER
                     HKANELFSWLHQFRCENGVYWTGYQFSLDLLWPDEQPTWTAGAVLLAADALAEATPAA
                     RLFTEVNLLEAAQQPQSAGHRELFE"
     gene            complement(2551..3258)
                     /locus_tag="PKI23_05855"
     CDS             complement(2551..3258)
                     /locus_tag="PKI23_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNN36498.1"
                     /translation="MLTVDLDRFALRPGDRVLDLGCGEGRHVISFYMAAEVLAVGIDL
                     GWNDLQTSQSRFRDFAEPDNPNKRLALLQGTALRLPFADGSFDTVVCSEVLEHIPDYR
                     GALAEIVRVLRPGGTFVASVPRFWPEKICWLLSDAYHEVEGGHVRIFDAGALQSDIER
                     LGFSCYDRHHAHALHSPYWWLQCLLWSRRDHSWLVKWYHKLLVWDLLKKPWLTQTAER
                     LLNPLIGKSVVMYFTKG"
     gene            complement(3245..4543)
                     /locus_tag="PKI23_05860"
     CDS             complement(3245..4543)
                     /locus_tag="PKI23_05860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNN36499.1"
                     /translation="MALASAVIERRPPLDAMAIERPLRIALLGYRSHPYCGGQGVYLR
                     FLSKALVEAGHSVDVISGPPYPQLDPRVRLVKLPSLDLFAQTSLFPHASLRALRDPIN
                     RFEYWGKLTGGFVEPETFGRRLVHHFRDRRQQYDLVHDNQSLCYGLLQLQQWGIPTVT
                     TIHHPITRDLEIALDSARNGWHRLLIRRWHSFLTMQKAVARELNHLVTVSECSRRDIA
                     EAFGLPIESINLVYNGIDTDEFRPCPELPREPFQIMATASADAPLKGLDHLLEAISRL
                     VPDYPALRLVVLGQPKPGGHTERLIERLRLADRVRFVRGLSTAEVAQLYARSTLAVVP
                     SLYEGFGLPAGEAMACGVPVISTTGGALPEVVGDAGVLVPPGDAVALASAIAELLEDP
                     ARREELGEAARRRIEQQFSWQVTARQMTDYYQTLLTHADR"
     gene            4785..5120
                     /locus_tag="PKI23_05865"
     CDS             4785..5120
                     /locus_tag="PKI23_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNN36500.1"
                     /translation="MQTLRNLPLLFLSLCVVLLLSLQGCTGNRYDRSTGERLDDAVIT
                     TRVKSNIIANPHLKFFDITVDTFRGVVQLGGFVDNEKMIRLAEKVTAETPGVVKVNNR
                     IAVKPANGH"
     gene            5174..5974
                     /locus_tag="PKI23_05870"
     CDS             5174..5974
                     /locus_tag="PKI23_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN36501.1"
                     /translation="MDLGIRNRVALVTGSHRGTGQVIAQRLATEGVRVLSHGPTADSE
                     PAAGCLGHLWGDLTTDEGAAQVVTQARALCGGVDILINNLGAAEAGQWGELTTADWLA
                     SYQLNVLSASRMIDAWLPGMKQQGWGRILQLGTLGTDRPGGSMPHYYAAKGALLTLTL
                     GLAQSLPASGITVNILSPGLILTPEVEASFRRRAQRQGWDGDWSQIEARSVQERHPNL
                     IGRIARRDEVADLALFLVSERASFITGQNIRIDGGALLCSASTTQGQV"
     gene            complement(5959..7296)
                     /locus_tag="PKI23_05875"
     CDS             complement(5959..7296)
                     /locus_tag="PKI23_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNN36502.1"
                     /translation="MRTEMDFLLLRRIEQRLFAEDDALPGWRRRLLWPTRLLYVMLRD
                     IFYGPLPLHATSLVYTTLLSLVPLMALGVAVIKLFGLPDQLLPLLHGFLQPLGERGET
                     VNRTVIDFIERIDIAPLGSIGLILLVYVAVTLIYKVERSLNLIWRVRRHRTFLQLIGH
                     CLLLVLLGPLLLVVLMGAIAVVMNSTLMHHLLALGAVSSLLLQLSWLLPVLLAALMIG
                     AVYLLVPNTSVRLSAAFTGSTVASLLWQLCGRIFSGFLISSAHYEAIYSGFAVLILLL
                     LWLFWSWLILLLGAAVAFYLQNPAQMKSERNELWVGGSLTRLLGLSIMHQLACSAQRK
                     EGVTLASLSERLQTPIWSVSAVVDLLGVAGLLTRQATAPEACRLACEPAQIELAALLR
                     LLDEGGEPSATPTVPLPAAVEQLHQRLMQAEQGAVGRMTLAELAATAEGQTCP"
     gene            complement(7305..7895)
                     /locus_tag="PKI23_05880"
     CDS             complement(7305..7895)
                     /locus_tag="PKI23_05880"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNN36503.1"
                     /translation="MSDLRPLRRELRRQRRALSASAQRQAAASVARHLAGWLRFLHSR
                     RIGHYLASDGEIDPTSALLRGQARGAQGFLPILHPFAHNRLLFAPHRPGERLILNRYA
                     IAEPDLRRQALTPAWSLDLVLLPLVAFDRSGARLGMGGGYYDRTFALDRPWPSRPLLV
                     GVAHHFQERPQLPMATWDVPLDAVVTDRELILIRCR"
     gene            complement(7901..8081)
                     /gene="ssrS"
                     /locus_tag="PKI23_05885"
     ncRNA           complement(7901..8081)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKI23_05885"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(8172..8477)
                     /locus_tag="PKI23_05890"
     CDS             complement(8172..8477)
                     /locus_tag="PKI23_05890"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNN36504.1"
                     /translation="MAEKGESCTLTIQILDKSFQVSCPVEERAALAESARYLDEKMRG
                     IRSSGSIIGMERIAVMAALNIAHDYLRSEQQRLLLEQGRQHLHDKLDVALTQLLPGE"
     gene            complement(8481..8693)
                     /locus_tag="PKI23_05895"
     CDS             complement(8481..8693)
                     /locus_tag="PKI23_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HNN36505.1"
                     /translation="MNHLDPAIADLERKIDLLIGRCEQLSKENARLRASESTWRGERT
                     RLVEQQHHARQQVVEMIKRLKALDQE"
     gene            8862..9383
                     /locus_tag="PKI23_05900"
     CDS             8862..9383
                     /locus_tag="PKI23_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNN36506.1"
                     /translation="MRERFAWLRLAALFATLGSSLPLLADDGRPIGEVSTVFKFLTPN
                     DKIKIHAFEDPAVEGITCYISRAVTGGLKGAFGVAEDTSDASISCHQTGPIRFRSAIA
                     AGKNGEEVFNERRSLLFKELHVTRFHDAQSNTLVYMTWSDRLIEGSPRNSISAVPLAA
                     WNGVAAQPARFDR"
     gene            complement(9392..10210)
                     /locus_tag="PKI23_05905"
     CDS             complement(9392..10210)
                     /locus_tag="PKI23_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNN36507.1"
                     /translation="MSDFLQALASQPFLRQALFAGLLASVACGVIGSYVVVKRIGYLA
                     GGIAHSVLGGMGVAYFFGGDPMDGALVAAVLAALLISWVNLHWREHEDTVIGALWAAG
                     MAVGILLMARTPGYNVDLMSYLFGNILLVPSSELWRMAWLDLIVLLLVLLFQKQFLAV
                     VFDEEFARLRGIRVAFYYPLLLVLIAVTVVVLIQMVGLILVIALLTLPAAVSGHYVRT
                     LGWMMVGATLLGMLFTFAGLALSYGPDLPAGATIILLASATYLISVVALAWRRR"
     gene            complement(10207..10971)
                     /locus_tag="PKI23_05910"
     CDS             complement(10207..10971)
                     /locus_tag="PKI23_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNN36508.1"
                     /translation="MNTEQLPLEIDRLRFSYGGIPLLEQIDLRVEPREFLALIGPNGS
                     GKSTLFKLILGLRQPDGGTIRLFGRPPREARRWVGYVPQYPLFNRDFPITVTQAVMLG
                     RLGLTRRWFGFNRADRLATARALDEVGISGLANRPIGNLSGGQLQRLLVARALVTEPR
                     LLLLDEPTANIDQQGEQGLFDLLGLLNQRMAIVVISHDIGFISQQVNRVACLNRTLIC
                     HPATELKSSDIEQMYGRPMHLIHHEHAHHTPGEGMR"
     assembly_gap    11053..11152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11153..11923)
                     /locus_tag="PKI23_05915"
     CDS             complement(<11153..11923)
                     /locus_tag="PKI23_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNN36509.1"
                     /translation="MAELRVAVAIAPQKYLLERLAGSRVKVLQVVPPGQGVETYEPTP
                     RLMSELDSSQLYFQLGLPFERVWLPKVRGLHPALQVVDVAAGIERLQMDGHHHGDSRQ
                     SEALADPHLWLAPGLLLRQAANMRDALMAADPAAAVHYQQGYGRLEQELTALDAELSA
                     QLASRRGASFMVYHPAFGYFADAYGLRQIAIEREGKSPGARSLAQVIEAGRQAGVRVI
                     LVEQQTDRRVAEMVADSLGARVVKVDHLAEDVPATLRQL"
     gene            complement(12139..12723)
                     /locus_tag="PKI23_05920"
     CDS             complement(12139..12723)
                     /locus_tag="PKI23_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNN36510.1"
                     /translation="MSQFTLPELPYAMDALQPHISKETLEFHYGKHHKTYVDKLNGLI
                     KGTEFEGKTLEQIIKSSSGGVFNNAAQVWNHTFYWHSLSPKGGGAPTGKIAEAINKAF
                     GSFDDFKAKFNDMAVNNFGSSWTWLVKKADGSLDIVNTSNAATPLTDANLTALLTVDL
                     WEHAYYIDYRNVRPDYLKGFWSLVNWEFVNQNLG"
     gene            complement(12836..13171)
                     /locus_tag="PKI23_05925"
     CDS             complement(12836..13171)
                     /locus_tag="PKI23_05925"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HNN36511.1"
                     /translation="MFRAPPRPVSAESPFASVHNVYERLVVEQIRQTLGFNSSENDSD
                     YLADVACVALNHLPPRYLRYEVDFLFYLSPQERAELEQKVAQAVNQAALHVANSRRSA
                     SGGYQQALG"
     gene            complement(13264..14181)
                     /gene="secF"
                     /locus_tag="PKI23_05930"
     CDS             complement(13264..14181)
                     /gene="secF"
                     /locus_tag="PKI23_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNN36512.1"
                     /translation="MADEKIIDFMGWRKPVGGFSIVLMVVAVLAIALRGFNLGLDFSG
                     GSLIELHFSQPADPQQVRDTLHGAGLDAVVQHFGSQNELLVRLHREFKAGLGEEVVQL
                     LDPQRSGSVTLTRAEFVGSQVGDELLQKGLLALFVALIAVLLYVAWRFPWKFAVGAVL
                     ALFHDVVVTLGAFALFQWEFDLTVLAAVLAVIGYSLNDSIVVGDRIRECFRKIRKESS
                     YRVINISITQTLDRTIFTSLTVILAVLALFFLGGDAIHGFSKALLIGVGFGTYSSVYV
                     ASSLLLLLRISREDFLVQVKEAAKDDGRP"
     gene            complement(14201..16048)
                     /gene="secD"
                     /locus_tag="PKI23_05935"
     CDS             complement(14201..16048)
                     /gene="secD"
                     /locus_tag="PKI23_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNN36513.1"
                     /translation="MVNRYPLWKNLLILAVTLYGFIYATPNFFPDQPAIQISRERSGE
                     LVTDGVVTQLGTALDAAGIGHQAAERLDNGSVMIRLQQRDQQLPAKAVIDQSLGEGHV
                     VALNLAPTTPQWLLAIGAKAMKLGLDLSGGVHFLMEVDTQAAIKQRQEIYVAELRQRL
                     REEKLRYRQLEPQVDGRLRLHFNTAEERTKAAEWVRGQYREFAVTEGGEGERLFLDLR
                     LTEAKVKEIADYAMEQNLTTLRNRVNELGVAEPLVQRQGSNRIVVELPGVQDTAQAKR
                     TLGKTANLEFRLEARDPAKGEQFAFRDERLGEARLERDVVTTGDSVSNAQSSYDENGR
                     PQVNIDLDSRGGKRMLDATRENVGRRLGVLFIEQKVRMVERERDGQRVREPVRVNERK
                     IISLATIQSPLGNSFRVTGLDSPQEAAELALLLRAGALAAPMVFVEERTVGPSLGQEN
                     IRLGTRSALIGIGLVMLFMVVVYKLFGLIANVALMVNLALLVAVMSLISATLTLPGIA
                     GIVLAMGMAVDANVLIFGRIREELKNGMSPQAAISAGFDRAFLTILDSNLTTLIATFI
                     LYLIGTGPVKGFAITLSIGIIVSMFTAVTGSRALINLIYGGRKVKKLLI"
     gene            complement(16123..16455)
                     /gene="yajC"
                     /locus_tag="PKI23_05940"
     CDS             complement(16123..16455)
                     /gene="yajC"
                     /locus_tag="PKI23_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNN36514.1"
                     /translation="MLSLISLAHAEGPAAPPPGAELINIAMLAGFVLLFYFIIWRPQS
                     KRAKEHKKLIDDLAKGDEVLTTGGLIGRVTKIEEGFVVLQVADNIELKFQRQAITASL
                     PKGTMKAI"
     gene            complement(16505..17611)
                     /gene="tgt"
                     /locus_tag="PKI23_05945"
     CDS             complement(16505..17611)
                     /gene="tgt"
                     /locus_tag="PKI23_05945"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNN36515.1"
                     /translation="MQFELLASDGAARRGRLRFPRGMVQTPAFMPVGTYGSVKGMMPR
                     DLEALGAEIILGNTFHLMLRPGTEVVRAHGDLHEFIGWPRPILTDSGGFQVFSLGQMR
                     RITEAGVRFRSPVDGAEVFLSPERSMQVQRELGADIVMIFDECTPYPATFEQARESME
                     LSLRWAARSRQAHGDNPAALFGIVQGGMYHALRTQSLAGLVEIGFDGYAIGGLSVGEP
                     KAEMYQTLAHCAAELPADRPRYLMGVGTPEDLVHAVCQGIDMFDCVLPTRNGRNGHLF
                     TSQGKVRIRNARYRHDTAPLDPQCGCYTCRHFSRAYLHHLDKCGEMLGATLNTLHNLH
                     YYQALMAGLRTAIEQGRLAAFVADFHGQQAIENG"
     gene            complement(17620..18678)
                     /gene="queA"
                     /locus_tag="PKI23_05950"
     CDS             complement(17620..18678)
                     /gene="queA"
                     /locus_tag="PKI23_05950"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNN36516.1"
                     /translation="MRRSDFHYHLPAELIARYPAPSRSGSRLLRLQADSGALSHHRFA
                     DLVALLRPGDLLVANDSRVIPARLYGRKPSGGRVELLVERMLSPRQALCHLRASKPLR
                     PGGVILLPEGVEAQLIDRQADLHRIELDGAISFAELLARHGEVPLPPYMARAAEPLDR
                     ERYQTLYARQEGSVAAPTAGLHFDQPLLSALAEAGIDWGTVTLHVGAGTFQPVRVDAL
                     DQHRMHSESIEVSPELVEQVARTRQRGGRVVAVGTTSVRALESAAQGGLLQPFSGETQ
                     IFIHPGVPFRVVDALITNFHLPESTLLMLVAAFAGHRQTMEAYRVAVEMGYHFFSYGD
                     AMLIEHGQLPWPRAGAAS"
     gene            18767..18853
                     /locus_tag="PKI23_05955"
     tRNA            18767..18853
                     /locus_tag="PKI23_05955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18801..18803,aa:Leu,seq:caa)
BASE COUNT         3718 a         6281 c         5709 g         3097 t
ORIGIN      
        1 cgcgatcacg gtgtcgccgg caccggtgac atcgtagccc tcccgcgcct gcgcccgcag
       61 gttgatcgga tctccctccc gcggtaccag cagcattccc tgcccgccac gggtcaccag
      121 cagtgcggtc agatcgagcc gcgccgtcat cgctcgcgcc ttgccgatca gctcggcttc
      181 atcggcgcag cgcccggcca cctgctcgaa ctcggcctgg ttgggcgtca gcagggtcgc
      241 accacggtag cggtcgaact cactcccctt ggggtcgacc agcaccggca tcgccaccgc
      301 ccgtgccgcc tggatcagcg gttgcggatc ggccagcgtc cccttggcat agtcggagag
      361 cagcagcacc tgactgcgtt tgagcgcctc gtgcagtgcc tgcgaagaga gcgctggcat
      421 cgggtcgaag cgctcctcga agtcgagccg cagcagctgc tggttgcggc tgatcactcg
      481 cagcttggtg atggtggggt gggcagccga atgctgaaag tgacagcgca cgccggcatc
      541 gaccaggatc tgcgtcaacc gctcacccat ctcatcgacg ccgacgacgc cggccagctc
      601 gaccgacaca cccagcgcgg cgaggttgcg ggcgacattg ccggcaccgc cggcgcgctc
      661 ctcgatccgg gtcacccgca ccaccggcac cggcgcctcg ggggacaccc gctgggtgtc
      721 gccatgccag tagcggtcga gcatcagatc acccaccacc agggcgcgca catgctcgaa
      781 cgggggcagg gaggctctca tggagtggtc ggtctcacgc aacatcggtt ggaacgtcct
      841 ggcataggcc caaatgagcc gctgccgtat tataggcgct ttggttttcg ggaatggtta
      901 tggaacaggt tgaattgccg atcgacatcg accaggtcaa gggctttctg caccctgacg
      961 aaggcgcagc gctctaccgg caggcgttga tcgccagccg ccttggacct tgcctggaga
     1021 tcggcagcta ctgcggaaaa tcgaccctct acctcgccgc cgcctgtcgt cagaccgacg
     1081 cgctgctcta tgccatcgat catcacggcg gttcggagga gcatcaactc ggcgaggagt
     1141 accacgatcc ggagctctac gaccgcgaag aggcggtgat ggacagcttc cgcacctttc
     1201 gccgcaacct gcgccgtgcc ggtctcgacg cacaggtggt accgatcgtc gccccctcgg
     1261 cactggcagc tcgccactgg aacacgccgc tcggcatggt cttcatcgat ggtggccaca
     1321 gcctggaagc agcgctgacc gattaccgct gctgggccgg ccacctcgtg ccgggcggca
     1381 ttctcgccat ccatgacatc tttcccgacc cgcgcgatgg cggtcaggcc ccccatcaga
     1441 tctatcagct tgcgagcgcc tcggggctat tcgaacagct cccgatggcc cgcactctgg
     1501 ggctgctgcg ccgcctctag cagattgacc tcggtgaaga gccgcgcggc cggggtcgcc
     1561 tcggccagcg catcggcggc cagcagcacc gctccggcag tccaggtggg ctgttcatcg
     1621 ggccagagca ggtcgaggct gaactggtag ccagtccagt agacgccatt ctcgcagcgg
     1681 aactggtgca gccagctgaa gagttcgttg gccttgtgcc gctcgcccgc cgccagcagc
     1741 gccagcgtca gctcgcagga ctccgccacc gtcacccagg gttcgtcgct gacgcagcgg
     1801 caaccgagct catgttcgac aaagctgctc cagcgcgtct gcaaccgttg ccgggcagcc
     1861 gcaccgcgca ccacgccccc cagaatcgga tagaaccagt ccatcgagta gcgtgtcttg
     1921 ctctgatcga accgctcggg gtggttgcgc agcgcatcgc ccagccgttc gcgcgccagt
     1981 gaccactgtg gcctggcatg gcccagcacc gtggcgatgt tgagggcaca ctccaggctc
     2041 ttgtgcaccg aactgcaacc ggtgaccagc gcctgcgcac ggggcactcc ttcggcatca
     2101 atcgcccagg ggatggtgcc acgccggcac tgcagggcca ccacatggtc gatggcccgc
     2161 tcgacggtgc gccacatgcg gcgcagaaag ctctcgtcgg tggtgatcag gtagtggtgc
     2221 cagacgccgg tggccacata ggcggtgaag ttgctctcct tgcgcgcctc ggcatccagc
     2281 accggctggc cctgttcata gcgtgcccac caactgccat cctccagctg ccactgccgc
     2341 agccagtcat aggcctgctc cgcctcctcc agatggccgg caatgctcag ccccatggcg
     2401 gcttcggtgt gatcccaggg gtcgaaatgg ccaccgtgaa accagggaat ggcgccgtcg
     2461 ctgcgttgca cgtcgagaat gtaatcgatt gacggacgaa aatagtcgag cggcagagcg
     2521 cctttggaga ggtataactg ggtcacgatc tcatcccttc gtgaagtaca tgacgacgct
     2581 ttttccaatc aacgggttga gcaggcgctc ggcggtttgg gtcagccaag gctttttcag
     2641 caaatcccac accaacagct tgtgatacca cttcaccagc caggagtggt cacgtcttga
     2701 ccagagcagg cattgcagcc accagtaggg gctgtgcagc gcatgggcat ggtggcggtc
     2761 gtagcagctg aatccaagcc gttcgatgtc ggactggagc gctccggcat caaagattcg
     2821 cacatgccca ccctcgacct cgtggtaggc atcgctcagc agccagcaga tcttctccgg
     2881 ccagaaacgc ggcacactgg cgacaaaggt gccgcccgga cgcaggactc tgacgatctc
     2941 cgccagcgcg ccccgatagt cgggaatgtg ctccagcacc tcggagcata ctacggtatc
     3001 gaaactgcca tcggcaaaag gcagccgcag cgcagtgccc tgcaacagcg caaggcgctt
     3061 gttcgggttg tccggttcgg caaagtcacg gaaacggctc tggctggtct gaagatcgtt
     3121 ccagccgagg tcgatcccca ccgccaacac ctcggctgcc atgtagaagg agatgacatg
     3181 ccgcccttcg ccacagccga ggtcgagcac ccggtcaccc ggtcgcagcg cgaaacggtc
     3241 gaggtcaacg gtcagcatgg gtcagcagcg tctggtagta gtcggtcatc tgccgggcag
     3301 tcacctgcca gctgaactgc tgctcgatgc gccggcgggc cgcctccccc aactcttcgc
     3361 gccgtgccgg atcctccagc aattcggcaa tggcactggc gagtgcgacg gcatcgcccg
     3421 gcggcaccag cacaccggca tctcccacca cttcgggcag cgcgccgccg gtggtcgaga
     3481 tcaccggcac gccgcaggcc atcgcctcac cggccggcag cccaaagcct tcatacagcg
     3541 atggcaccac cgccagcgtg gagcgtgcat agagctgcgc cacctctgcg gtcgagagcc
     3601 cgcgcacgaa acgcacccgg tcggccagcc gcagccgttc gatcagccgc tcggtgtgac
     3661 cgcccggttt gggttgcccc agcaccacca gccgcagcgc cggatagtcc ggcaccagcc
     3721 gggaaatcgc ctccagcagg tggtcgagcc ccttgagcgg agcatcggca ctggccgtcg
     3781 ccatgatctg gaacggctcg cgcggcagtt ccggacaggg tcggaattcg tcggtgtcga
     3841 tgccgttgta gaccaggttg atcgactcga tcggcaagcc aaaggcctcg gcaatgtcac
     3901 ggcgagagca ctccgaaacc gtcaccagat ggttcagctc ccgcgccacc gccttctgca
     3961 tggtcaaaaa ggagtgccag cgccggatca gcagtcgatg ccagccgttg cgggcgctgt
     4021 cgagggcaat ctccagatca cgggtgatcg gatggtgaat ggtggtgacg gtgggaatgc
     4081 cccactgttg caactgcaac agcccgtagc agaggctctg gttgtcatgc accagatcat
     4141 actgctggcg gcgatcgcga aagtggtgga ccaggcggcg cccgaaggtc tccggttcga
     4201 cgaaaccgcc agtcagcttg ccccagtatt cgaagcggtt gatcggatcg cgcagcgcgc
     4261 gcaggctggc atggggaaat agagaggtct gcgcaaaaag atcaaggctg ggcagcttga
     4321 ccagcctgac tcgcggatcg agttgcggat agggggggcc ggagatcaca tcgacgctgt
     4381 gaccggcctc gaccagcgcc ttgctgagaa agcgcaggta gaccccctgc cctccgcaat
     4441 aggggtgact gcggtagccc agcagcgcga tgcgcagcgg acgctcgatg gccattgcat
     4501 cgagcggtgg tcgtcgttca atcactgccg aagccagcgc catcaggtat ccatacatcg
     4561 atcaggtcga actgccaccc ggcatggtca tccctcgccg catcagggct ggtatgaaaa
     4621 aacgtgccag aaccgggcac ttgagccggt ggattctact tgactgatca acaatcaggc
     4681 aagaaacaag cagttacggc ttggcagcag accttgattt gacctaggat aacgaccgtc
     4741 gccgctgtgc ggtaccccct ccactccact gaaaacgagc caccatgcag accctgcgca
     4801 acctaccgct tctgttcctt tcgctctgtg tcgtcctgct gctgtcgctg cagggttgta
     4861 ccggcaaccg ctatgaccgc agcaccggag agcggctcga tgatgcggtc atcaccaccc
     4921 gggtgaagtc gaacatcatc gccaacccgc acctgaaatt cttcgacatc accgtcgaca
     4981 ccttcagggg cgtggtgcag ttgggcggct ttgtcgacaa cgagaagatg attcggttgg
     5041 ccgagaaggt gaccgccgag actccgggcg tggtcaaggt gaacaaccgg atcgccgtca
     5101 agcctgccaa cggccactga cccggcacag cggtcaagac ctctccgaaa cggcaccaca
     5161 cagggcccgc ctcatggatc tgggcattcg caaccgggtg gcgctggtca ccggcagcca
     5221 ccgcggcact ggccaggtga ttgcgcagcg gctggccacc gagggggtca gggtcctgag
     5281 ccacggtccc acggccgaca gcgaacccgc cgcgggctgc ctcggccatc tgtggggcga
     5341 tctgaccacc gacgaaggtg ccgctcaggt ggtgacacag gccagggcgc tgtgcggtgg
     5401 agtcgacatc ctgatcaaca acctcggcgc cgccgaagcg ggccagtggg gcgagttgac
     5461 caccgccgac tggttggcga gctatcagct caatgtgctg tcggccagcc gcatgatcga
     5521 tgcctggctg ccgggcatga agcagcaagg ctggggccgg atcctgcaac tgggcaccct
     5581 cggcaccgac cggcctggtg gcagcatgcc gcactactac gctgccaagg gggcgctgct
     5641 gaccctcacg cttggtctgg cccagagcct gccggccagc ggcatcaccg tcaacatcct
     5701 ctcgcccgga ctgattctga cgccggaggt cgaggccagc ttccgccgcc gggcccaacg
     5761 tcagggctgg gacggtgact ggtcgcagat cgaggcgcgc agcgtgcagg agcgccatcc
     5821 caacctgatc ggccgcatcg cccgtcgtga cgaagtggcc gatctggcac tgttcctggt
     5881 cagtgagcgc gccagcttca tcaccgggca gaacatccgc atcgatggcg gtgcgctgct
     5941 gtgcagtgcc agcaccactc aggggcaggt ctgaccttca gctgtcgccg ccagctccgc
     6001 aagcgtcatg cgtcccactg ccccctgctc ggcctgcatc agccgctgat ggagctgctc
     6061 gacagccgcc ggcaatggta cggtcggcgt tgctgacggc tcacccccct cgtccagaag
     6121 acgcagcagt gccgccagct cgatctgcgc cggttcacac gccagccggc aggcttcggg
     6181 cgcggtggcc tggcgtgtca gcagacccgc gacccccagc aggtcgacca ccgcgctgac
     6241 actccagatc ggtgtctgca accgctcgct cagtgacgcc agcgtcacgc cctccttccg
     6301 ctgcgcgctg cacgccagct gatgcatgat cgacaggccg agcagacggg tcagactgcc
     6361 gccgacccac agctcgttgc gctcgctctt catctgcgcc ggattctgca gataaaaggc
     6421 gacagcggct ccaagcagca ggatcagcca gctccagaac agccacaaca gcagcagaat
     6481 cagcaccgcg aagcccgaat agatcgcctc gtagtgggcc gatgagatca aaaagccgga
     6541 gaagatccgt ccacacagct gccacagcag gctggccacc gtcgaaccgg tgaatgccgc
     6601 agagagccgg accgaggtgt tgggcaccag cagatagaca gcgccgatca tcagcgccgc
     6661 caacaacacc ggcagcagcc aggagagttg cagcagcagg ctgctcaccg cccccaacgc
     6721 cagcagatgg tgcatcaggg tgctgttcat caccacggcg atcgcaccca tcagcacgac
     6781 cagcagcagc ggacccagca gcaccagcag cagacagtgg ccgatgagct gcaaaaaggt
     6841 gcgatggcgc cgcacccgcc agatcagatt gagcgaccgt tcgaccttgt agatcagcgt
     6901 gacggcgaca tagaccagca ggatcagacc gatcgaaccg agcggtgcga tgtcgatgcg
     6961 ctcgatgaag tcgatgacgg ttctgttcac cgtctcgccc cgctcaccga gcggctgcaa
     7021 aaacccatgc agcagcggca gcagctgatc cggcaatcca aacagcttga tcaccgcgac
     7081 gcccagcgcc atcagcggca ccagtgacaa cagcgtggtg tagaccagac tggtggcgtg
     7141 cagaggaagt ggaccgtaga agatgtcacg cagcatgacg tagagcagcc gtgtcggcca
     7201 cagcagtcgg cgccgccagc ccggcagagc gtcatcttca gcaaagagcc gctgctcgat
     7261 ccggcgcaac agcagaaaat ccatctcggt ccgcatcggc cgggtcacct gcaccggatc
     7321 agaatcagct cccggtcggt caccaccgca tcgagcggga catcccaggt cgccatcggc
     7381 agttggggcc gctcctgaaa atggtgggca acgcccacca gcaacgggcg cgatggccag
     7441 gggcgatcca gcgcaaaggt ccggtcatag tagccgcccc ccatccccaa ccgcgctcca
     7501 ctgcggtcga acgccaccag cggcagcaac accagatcga gcgaccaggc cggcgtcagc
     7561 gcctgccggc gcagatcggg ctccgcaatg gcataacggt tcagaatcag ccgctcaccg
     7621 ggccggtgcg gcgcaaacag cagacggttg tgggcaaagg ggtgcaggat cggcagaaaa
     7681 ccctgcgcac cacgggcctg gccacgcagc agtgcgctgg tcggatcgat ctcgccatcg
     7741 ctggccagat agtgaccgat gcgtcggcta tggagaaaac gcagccagcc ggccagatgg
     7801 cgcgcgaccg aagcggcagc ctggcgctgc gcggaagcag agagggcgcg acgctggcga
     7861 cgcagttcgc gccgcagtgg ccgcagatca ctcatgcagg gagctccccg agaacgccgc
     7921 tgtcggtaga gcccttgaac caatagagtc caaggcggcg gtcactgagg tgaatcaggc
     7981 tttccgacca gcggacatgc acaccatcac ctgctggcga cctccagttt tcagagaatc
     8041 ggctcgggga catacccgac tggccaacgc cccagggagc tggcttgagt atagcagcgt
     8101 cagcgccaaa gcagcatctt tcgagaatcg gaaaaccgga cgggcatctt cacactgcac
     8161 cgtgcggcaa ctcattcgcc aggcagcagc tgtgtcagcg ccacatcgag cttgtcatgc
     8221 agatgttgcc ggccctgctc cagcagcagg cgctgctgct cgctgcgcag gtagtcgtgg
     8281 gcgatgttca gcgccgccat caccgcgatg cgctccatgc cgatgatcga accgctgctg
     8341 cgaatgccgc gcatcttctc atcgagatag cgggcggact ccgccagcgc cgcgcgctcc
     8401 tccaccggac agctcacctg aaacgatttg tccaggatct ggatggtcag cgtacagctt
     8461 tcgccctttt cagccatgac tcactcctga tccagcgcct tgagccgttt gatcatctcg
     8521 accacctgct gccgggcatg gtgctgctgt tcgaccagcc gcgtccgctc accacgccag
     8581 gtcgattcac tggcgcgcag ccgggcattc tccttgctca actgctcgca acggccgatc
     8641 agcaggtcga ttttgcgttc aagatcagca atggccgggt caaggtggtt catgaggctc
     8701 tttcagggcg atgcagaatc gattcgatca actatagagc gccgggcacc atcgatcaat
     8761 cgctgcacgg cgatggcgtc aacgatgctg cttgccaccg cttgcatcgt ccgccgcgca
     8821 aaactaagct cgcgccaccg cttcgacagg tggatgcttc gatgagagag cgcttcgcat
     8881 ggctgcgcct ggcagccctt ttcgccaccc ttggcagcag cctgccactg ctggccgatg
     8941 atggacgccc gatcggcgag gtcagtacgg tgttcaagtt cctgaccccg aatgacaaga
     9001 tcaagatcca tgcgttcgag gatcccgccg tggaggggat cacctgctac atcagccggg
     9061 cggtgactgg cggactcaag ggggcgttcg gcgttgccga agacacctcc gatgccagca
     9121 tctcctgcca tcagaccggc ccgatccgct ttcgaagcgc gatcgccgcc ggaaaaaatg
     9181 gcgaggaggt gttcaacgag cgccgttcgc tgctgttcaa ggagctgcat gtcacccgtt
     9241 ttcatgacgc ccagagcaac acgctggtct acatgacctg gagcgaccgg ctgatcgagg
     9301 gctcgccgcg caattcgatc tcggcggtgc cgctggccgc ctggaatggt gtcgccgccc
     9361 agccagcgcg gttcgatcgc tgacttcgac ctcagcgtcg ccgccatgcc agcgcaacca
     9421 ccgagatcag ataggttgca ctggccagca ggatgatggt cgcaccagcc ggcagatcag
     9481 gtccgtagga gagcgccagc ccggcaaagg tgaagagcat gcccagcagg gtggcaccca
     9541 ccatcatcca gccgagcgtg cgcacatagt ggccactgac ggcggcgggc agcgtcagca
     9601 gcgcaatcac cagaatcagc cccaccatct ggatcagcac caccacggtc accgcgatca
     9661 gcaccagcag cagcgggtag tagaacgcca ccctgatgcc acgcaaccgg gcgaactcct
     9721 cgtcaaagac caccgcaaga aactgcttct ggaacagcag caccagcagc agcacgatca
     9781 ggtcgagcca ggccattcgc cacagctcag aactcggcac cagcagaatg ttgccgaaca
     9841 ggtagctcat cagatcgacg ttgtagccgg gcgtgcgcgc catcagcagg atgccgacgg
     9901 ccatccctgc tgcccacagt gcgccgatca cggtgtcctc atgttcgcgc caatgcagat
     9961 tgacccaact gatcaacagc gccgccagca ccgccgcgac cagtgcgcca tccatcgggt
    10021 cgccaccgaa gaagtaggca acccccatcc cgcccagcac cgaatgggca ataccgccgg
    10081 ccaggtaacc gatgcgcttg accaccacat agctgccaat cacgccacag gccacgctgg
    10141 ccagcaggcc ggcgaacagt gcctgccgca gaaagggctg gctggccagc gcctgcagaa
    10201 aatcgctcac cgcatcccct cgcccggcgt gtggtgggca tgttcatggt ggatcaggtg
    10261 catcggacgt ccatacatct gctcgatgtc actgctcttg agctcagtgg ccggatggca
    10321 gatcagcgtg cggttgaggc aggcgacccg gttcacctgc tgcgagatga agccgatgtc
    10381 atgcgagatc accacgatgg ccatgcgctg attgagcagc cccagcaggt cgaacagccc
    10441 ctgttcgccc tgctggtcga tgttggcggt cggctcatcc agcagcagca gcctgggttc
    10501 ggtcaccagc gcacgcgcca ccagcagccg ctggagctgc ccgccggaga ggttgccgat
    10561 cggacggttg gccagaccgc tgatgcccac ctcgtcgagg gcacgggcgg tggccagccg
    10621 atcggcccgg ttgaaaccga accagcgccg cgtcagcccc agccgaccca gcatcactgc
    10681 ctgggtgacg gtgatcggaa agtcgcgatt gaacagcgga tattgcggta catagccgac
    10741 ccagcggcgc gcttcacgtg gcgggcggcc gaagagccga atcgtgccac cgtcgggctg
    10801 ccgcaatccc agaatcagct tgaacagggt gctcttgccg ctgccattgg gaccgatcag
    10861 cgccagaaac tcacgcggct cgacccgcag atcgatctgc tcaagcagcg gaattccgcc
    10921 ataggagaag cggagccggt cgatctcgag cggcaattgt tcggtattca tggcgtggag
    10981 cctgtcagca gggcactcaa ctggcgcagc gtggccggca catcctcggc caggtggtcc
    11041 accttgacga cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaactggc
    11161 gcagcgtggc cggcacatcc tcggccaggt ggtccacctt gacgacacgg gcaccgaggc
    11221 tgtcggccac catttcagcc acccgccgat cggtctgctg ctcgaccaga atcacgcgca
    11281 caccggcctg ccgacccgct tcgatcacct gggccagcga gcgggcaccg ggagacttgc
    11341 cctcgcgctc gatggcaatc tggcgcagtc cataggcatc ggcaaagtag ccaaaggccg
    11401 gatggtagac catgaaactg gcgccacgcc gggaggccaa ttgtgcactc aactcggcat
    11461 cgagggcggt cagctcctgc tccagccggc cataaccctg ctgatagtgc acagccgcgg
    11521 ccggatcggc ggccatcagt gcatcgcgca tgttggccgc ttgacgcagc aacagtccgg
    11581 gcgccagcca gagatgtgga tcggcaagtg cctctgactg ccgtgaatca ccatgatgat
    11641 gcccatccat ctgcagccgc tcgatgccgg cggcgacatc gaccacctgc aacgccggat
    11701 ggagcccacg caccttgggc agccagacac gctcgaaggg caggccaagc tgaaagtaga
    11761 gctggctgct gtccagttcg ctcatcaacc gcggggttgg ctcgtaggtc tcgacgccct
    11821 gccccggcgg caccacctgc agcactttca cccgcgaacc ggccaggcgt tcgagcagat
    11881 acttctgcgg tgcgatggcg accgccaccc gcaattcagc caccgcgaac gaactggcca
    11941 ggagccacaa aaaaaacagt gcaaagcggc gacccatgcg tggccttgac ccaaaagttt
    12001 tcattatatc cattcacgag ataaagccgt cgccttcgcc gatggcgcac agcgcatgct
    12061 cctgaaaaaa gaaaccccga cggtcgccgg ggtttcatcg acagccgaac cgcacgggcg
    12121 cggtcagcgc aacgggggtc agccgagatt ctggttgacg aactcccagt tgaccagcga
    12181 ccagaagccc ttcaggtaat cggggcggac gttgcgatag tcgatgtagt aggcatgttc
    12241 ccacagatcg accgtcagca gcgcggtcag gttggcatcg gtcagcggcg tggcagcgtt
    12301 gctggtgttg acgatgtcga gtgatccatc ggcctttttg accagccagg tccagctgga
    12361 accgaagttg ttgaccgcca tgtcattgaa cttggccttg aaatcatcaa aggagccgaa
    12421 cgccttgttg atggcctcgg caatcttgcc ggtgggcgca ccgccacctt tcgggctcag
    12481 gctgtgccaa tagaaggtgt ggttccacac ctgcgcggca ttgttgaaga caccgcccga
    12541 tgaactcttg atgatctgtt cgagcgtctt gccttcgaac tcggtgccct tgatcaggcc
    12601 gttgagcttg tcgacatagg tcttgtgatg cttgccgtag tggaactcca gcgtctcttt
    12661 ggagatgtgc ggctgcaggg catccatcgc gtagggcaat tctggcaggg taaactggct
    12721 catgctgtga ttctccttcg actcaccagt ggttaatggc ccagctattg tatcaactat
    12781 cccccgccat ggcgcctgcc gattcgattg agtgccgctg tcgcgccagc ccccgtcaac
    12841 ccagtgcctg ctgatagcca ccgctggccg aacggcgcga attggcgaca tgcagcgcgg
    12901 cctggttcac cgcctgcgcc accttctgct cgagttcggc gcgctcctgt ggcgagaggt
    12961 agaacagaaa gtccacctcg tagcgcagat agcgcggcgg caggtggttg agcgcgacac
    13021 aggccacatc ggccagatag tcgctgtcgt tttcgctgct gttgaagcca agggtctgcc
    13081 gaatctgttc gacgaccagc cgctcgtaga cgttgtgaac cgaggcaaag ggcgattctg
    13141 ccgacacggg acggggtggc gcacggaaca tggtgagttt tctccggtga acaggacatt
    13201 gatcctcacc gaagtgtagg ccagaagcgc cacttgcgcc cggcgcctca agacgatggc
    13261 cggtcacggc cggccatcgt ccttggccgc ctccttgacc tgaaccagaa aatcctcgcg
    13321 gctgatcctg agaagcagca acaggctcga cgcgacatag accgaggagt aggtgccgaa
    13381 ccccacaccg atcagcagtg ccttggagaa gccatgaatc gcatccccgc ccagaaagaa
    13441 cagcgccagc accgccagaa tcacggtcag ggaggtgaag atggtccggt cgagcgtctg
    13501 ggtgatggag atgttgatca cccgataaga gctctccttg cgaatcttgc ggaagcattc
    13561 gcgaatccgg tcaccgacca cgatgctgtc attgagcgag tagccgatga ccgccagcac
    13621 tgccgccagc acggtgaggt cgaactccca ctggaagagc gcaaaggcac cgagggtgac
    13681 caccacgtca tggaacagcg ccagcaccgc accaaccgcg aacttccacg gaaagcgcca
    13741 ggcgacatag agcagcacgg cgatcagcgc gacaaagagc gccagcaacc ctttctgcaa
    13801 cagctcatcg cccacctgtg aaccgacgaa ttcagcccgc gtcagagtga cgctgccgct
    13861 gcgctgcgga tcaagcagtt gcacgacctc ttcacccagc ccggccttga actcccggtg
    13921 cagccgcacc agcagctcat tctgactgcc gaagtgctgc accacggcat cgagacccgc
    13981 accatgcagg gtgtcgcgca cctgttgcgg atcggcgggc tggctgaagt gcagctcgat
    14041 cagtgagcca cccgaaaagt cgagccccag attgaagcca cgcagcgcaa tcgccagcac
    14101 cgccaccacc atcagaacga tggagaagcc gccgaccggc ttgcgccagc ccatgaaatc
    14161 aatgatcttt tcatccgcca tccggccgct gctcctggta tcagatcaac agttttttca
    14221 ctttgcgccc accgtagatc aggttgatca gcgcccgtga accggtgacg gccgtgaaca
    14281 tcgagacaat gatgccgatc gacagggtga tggcaaagcc ctttaccggt ccggtgccga
    14341 tcagatagag gatgaaggtg gcgatcaggg tggtcaggtt ggagtcgaga atggtcagaa
    14401 aggcccggtc gaagccggcg ctgatcgccg cctgcggtga catgccgttc ttcagctcct
    14461 cgcggatacg gccaaagatc aacacgttgg catcgaccgc catccccatc gccagcacga
    14521 tgccggcaat gccgggcagc gtgagggtcg ccgagatcag cgacatcacc gccaccagca
    14581 gcgccagatt caccatcagg gccacattgg caatcaggcc gaacagcttg tagaccacca
    14641 ccatgaacag catcaccaga ccaatgccga tcagcgccga ccgggtgccc agacggatgt
    14701 tctcctgccc caggctcggg ccgacggtgc gctcctcgac aaagaccatc ggcgcggcca
    14761 atgcgccggc gcgcagcagc agtgccagtt cggccgcctc ctgcggactg tcgagaccgg
    14821 tgacccgaaa gctgttgccg agcggcgact gaatggtggc cagcgaaatg atcttgcgtt
    14881 cgttgacccg aaccggctcg cgcacccgct gcccatcgcg ttcacgctcg accatgcgca
    14941 ccttctgctc gatgaagagc acgcccagac ggcgaccgac attttcgcgc gtcgcatcga
    15001 gcatgcgctt gccgccacgg ctgtcgaggt cgatgttgac ctgcggccgg ccattctcgt
    15061 cataggagga ttgcgcattg gagacgctgt cgccggtggt gaccacatca cgctccagcc
    15121 gcgcctcgcc gagccgttcg tcacggaagg cgaactgctc gcccttggcc ggatcgcgcg
    15181 cctccaggcg gaactccagg ttggcggtct tgccgagggt ccgcttggcc tgcgcggtgt
    15241 cctgcacgcc gggcaactcc accacgatgc ggttgctgcc ctggcgctgc accagcggtt
    15301 cagccacgcc cagctcattc acccggttgc gcagggtggt gaggttctgc tccatcgcat
    15361 agtcggcgat ctccttcacc ttggcttcgg tcaaccgcag atcgagaaag agccgctccc
    15421 cctcgccccc ttcggtcacc gcgaattcac ggtactgccc gcgaacccac tccgccgcct
    15481 tggtgcgctc ctcggccgtg ttgaagtgca gccgcaaccg cccatccacc tgcggctcca
    15541 gctgccggta gcgcagcttc tcttcgcgca gccgctggcg cagttcggcc acatagatct
    15601 cctgccgctg cttgatcgcc gcctgggtat ccacctccat caggaagtgc acaccgccac
    15661 tgaggtcgag ccccagcttc atcgccttgg caccaatggc cagcaaccac tgcggcgtgg
    15721 tcggcgccag gttgagggcc actacatgcc cctcgcccag gctctgatcg atcaccgcct
    15781 tcgccggcag ttgctggtcg cgctgctgca ggcggatcat cacactgccg ttgtcgagcc
    15841 gctcggctgc ctgatggcca atgcctgccg catcgagtgc agtgccaagc tgggtgacca
    15901 ccccatcggt caccagttcg ccactgcgct cgcgcgagat ctggatggct ggctggtcag
    15961 gaaagaagtt cggcgtggca tagatgaacc catagagggt cacggccaga atcagcagat
    16021 tcttccaaag cggataacgg ttcaccatgc caccctgtgc agctcgtccg atcagaatgc
    16081 agaaaaaagg atagaagagg gccgagcggt cgccttccgg actcagatcg ccttcatcgt
    16141 gcccttgggc agcgaggcag tgatggcctg gcgctggaac ttcagttcga tgttgtcagc
    16201 cacctgcaac accacgaacc cctcttcgat cttggtgacc cgaccgatca gcccgccggt
    16261 ggtgagcacc tcatcgccct tggccagatc atcgatcagc ttcttgtgct ccttggcccg
    16321 cttgctctgt ggccgccaga tgatgaagta gaacagcaac acgaagccgg ccagcatggc
    16381 gatgttgatc agctccgcgc caggcggcgg cgcggcgggt ccttctgcat gcgccaaaga
    16441 aatgagagac agcattgatt gacagacctt tgatgaacaa taacaagagg ttggataaat
    16501 tttattatcc gttttcgatg gcctgctggc catgaaagtc ggcgacaaag gcggctaatc
    16561 taccttgttc gatggcggtg cgcaaaccag ccatcagcgc ctgatagtag tgcaggttgt
    16621 gcaatgtatt gagcgtcgca ccgagcatct cgccacattt gtccagatgg tgcaggtagg
    16681 cacggctgaa gtggcgacag gtgtaacaac cgcactgggg atcaagcggc gccgtgtcat
    16741 gccgatagcg ggcattgcga atccgcacct tgccctgact ggtgaagagg tgaccattgc
    16801 gaccattgcg ggtcggcaac acgcagtcga acatgtcgat gccctggcag acggcatgga
    16861 ccagatcctc gggcgtgccg acacccatca gatagcgggg ccgatcggcc ggcaactcgg
    16921 cggcgcagtg cgccagcgtc tgatacatct ccgccttggg ctcaccaacc gacaggccac
    16981 cgatcgcata gccgtcaaaa ccgatctcga ccagcccggc cagcgactgc gtgcgcagcg
    17041 cgtggtacat ccccccctgc acgatgccga acagcgccgc cggattgtcg ccatgggcct
    17101 gcctgctgcg cgcggcccag cgcagcgaca gctccatcga ctcacgcgcc tgctcgaagg
    17161 tggccggata gggtgtgcac tcgtcaaaga tcatgacgat gtccgcaccc agttcgcgct
    17221 gcacctgcat cgaccgctcg ggcgacagaa agacctcggc accatcgacc ggtgaacgaa
    17281 agcgcacacc cgcctcagtg atgcgccgca tctgccccag gctgaacacc tgaaaaccgc
    17341 ccgaatcggt caggatcggc cgcggccagc cgatgaactc atgcagatcg ccatgggcgc
    17401 gcaccacctc ggtgccagga cgcagcatca gatgaaaggt gttgccgagg atgatctcgg
    17461 cgcccagcgc ctccagatca cgcggcatca tccccttgac cgacccatag gtgccgaccg
    17521 gcatgaaggc gggcgtctgc accatcccgc gcggaaagcg caaccgcccc cgccgcgccg
    17581 cgccgtcact ggccagcagc tcgaactgca tgaagctgct cacgaagcgg ctccggcgcg
    17641 cggccagggc aactgcccat gctcgatcaa catcgcatct ccatagctga aaaagtgata
    17701 acccatctcg accgcgaccc ggtaggcctc catcgtctgc cgatggccgg caaaggccgc
    17761 caccagcatc agcagcgtcg actcgggcag atgaaagttg gtgatcagcg catccaccac
    17821 ccgaaagggc acgccggggt ggatgaaaat ctgcgtctcg ccactgaagg gctgcaagag
    17881 cccaccctgc gccgcgctct ccagtgcgcg cacgctggtg gtgccgaccg ccaccacccg
    17941 cccgccacgc tgtcgggtgc gcgccacctg ctcgaccagt tccggcgaca cctcgatcga
    18001 ctcgctgtgc atgcgatgct gatcgagcgc atcgacccgc accggctgaa aggtccccgc
    18061 ccccacatgc agggtcacgg tgccccaatc gatgccggcc tcggccagcg cgctcaacag
    18121 cggctgatcg aagtgcaacc cggcggtcgg cgccgccacc gaaccctcct gccgggcata
    18181 gagggtctga tagcgctcgc gatcgagcgg ctcggccgcg cgcgccatgt agggcggcag
    18241 cggcacctcg ccatgccgcg ccagcagctc ggcaaagctg atggcgccat cgagctcgat
    18301 ccgatggaga tccgcttgcc ggtcgatcaa ctgcgcctcg accccttcag gcagcagaat
    18361 cactccaccg ggccgcagcg gcttgctggc ccgcagatgg cacaatgcct gtcgcggcga
    18421 caacatccgc tccaccagca gctccacccg gccgccactc ggctttcggc cataaagacg
    18481 cgccggaatc acccggctgt cattcgccac cagcagatca cccgggcgca gcagcgcgac
    18541 cagatcggca aaacggtgat gactgagcgc accgctgtcg gcctgcaacc gcagcagacg
    18601 gctgccgctg cgcgatggcg ccggatagcg ggcaatcagt tcggcgggca gatggtagtg
    18661 aaaatcgctg cgtcgcatgg cgggccgatc gaaaaatggc gcagactaat gcaaaagtgc
    18721 aggtgactcc agaacggcgc ctgctataat cggcgccgct cagatggccg agatggcgga
    18781 acaggtagac gcgccagact caaactctgg tggtggcaac accgtgtcgg ttcgattccg
    18841 actctcggca ccagacatcc tttcgctcca gtgcgaatca ttccacaagc ccgtgtcatt
    18901 gcggg
//
LOCUS       DAORCQ010000040        18720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1793_length_18720_cov_5.621752, whole genome shotgun sequence.
ACCESSION   DAORCQ010000040 DAORCQ010000000
VERSION     DAORCQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_18720_cov_5.621752"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="PKI23_05960"
     CDS             <1..127
                     /locus_tag="PKI23_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HNN36517.1"
                     /translation="AAIIGDKVRVTGKKRDDLQQAIALLRAAEFELPLQFDNFRD"
     gene            complement(137..1144)
                     /locus_tag="PKI23_05965"
     CDS             complement(137..1144)
                     /locus_tag="PKI23_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN36518.1"
                     /translation="MKKFLILLRARNAEFYRDRASLTWNLLLPLMLVVGFVFAFSDPR
                     QDLFKVGVYAPAGGADRTALAQLKHIQIVPFDNLDKAISRVQHHQIDLLVAEGDAVRY
                     WVNETSGKGYFVERLLRQEEKRALVKEVVQGREVRYLDWVMPGIISMNMMFGALFGVG
                     YVIVRYRKNGVLKRLQATPVTPLQFICAQVLSRLSILLIIAVIIYAGCALFLDFLMLG
                     SRLTLLLVAALGGLCMIALGLLVASRTESEELASGLLNFATWPMMFLSEVWFSLDGAH
                     PVLKWLAELFPLTHLVTAARAIMVDGATLSEVMHHVTVLGGMTLLFLTLGALLFRWGK
                     D"
     gene            complement(1159..2043)
                     /locus_tag="PKI23_05970"
     CDS             complement(1159..2043)
                     /locus_tag="PKI23_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN36519.1"
                     /translation="MTPILEVRELVKVYPKVKAVNGISFAIEAGCCFGLLGPNGAGKT
                     TTIEMIEGIKTPTSGEILYRGQPRTRHFKQEAGIQFQSTALMDFITVEEILRLFASFY
                     PRALPVDAVVEMCDLGEFKNQYATRLSGGQKQRLLLGLALIHDPEIVFLDEPTTGLDP
                     QSRRRFWDLINDIKARGKTVVLTTHYMEEAERLCDRLVIIDHGRIIEQGAPQELLRRH
                     FGHVRVCLDRLTLTPEVEALPEPVSVTAEGVEILSQSVEATLARLIAAGVPLDSLRVR
                     NPTLEDLFIKLTGHQLRE"
     gene            complement(2040..3260)
                     /locus_tag="PKI23_05975"
     CDS             complement(2040..3260)
                     /locus_tag="PKI23_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HNN36520.1"
                     /translation="MLITLMMGFSSGLPLLLTLSLLQVWLRSSGVDLTTIGLFALVGL
                     PYTLKFLWAPLLDRYSPWPLGRRRGWLLWTQSTLMVAIALLGNMDPQDNLTALALVAL
                     LVAFCSATQDTLIDAYRREHLPETELGLGASAYTYGYRIGMLLASGGGLILAGQIGFA
                     AVYWVMALLMGAGLLTTLLAPEPAAPAVQSGGLRAMVVEPFRDFFMRADAWWVLAFIL
                     FYKLGDTLASAMTSPFYVDLGYSPAEIGAVVKLFGFSATLLGALLGGVWVLRVGIRTA
                     LWHFGFAQMLSTLGFVLLTRLGHSPGGLALVVATENLTAGMGTAAFLAFMASQTNQRY
                     TATQYALFSSIMGIPRTLLAAPTGWMAELLGWSGFFILCTLLAVPGFWLLKQLEGWLT
                     TPASQPVNVETSLP"
     gene            3615..3905
                     /gene="groES"
                     /locus_tag="PKI23_05980"
     CDS             3615..3905
                     /gene="groES"
                     /locus_tag="PKI23_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNN36521.1"
                     /translation="MKIRPLHDRVVVRRMEEETKTAGGIVLPGSAAEKPSQGEVIAVG
                     KGKALDNGEIRPMDVKVGDRILFGQYSGSTVKIDGKELLVMSESEIFAVLES"
     gene            3964..5601
                     /gene="groL"
                     /locus_tag="PKI23_05985"
     CDS             3964..5601
                     /gene="groL"
                     /locus_tag="PKI23_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299861.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNN36522.1"
                     /translation="MAAKDVLFGDNARKKMLNGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASRTSDEAGDGTTTATVLAQAIVRE
                     GLKADAAGMNPMDLKRGIDKAVAKAVEAVKQMAQPCTDDKAIAQVGTISANSDSSIGE
                     IIAEAMKKVGKEGVITVEEGSGLENSLDVVEGMQFDRGYLSPYFINNQESMSAELDNP
                     YILLHDKKISSIRDMLPVLEAVAKSGRPLLIICEDVEGEALATLVVNTMRGIVKVCAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEELGLALEGTTLQDLGQAKRVNISKENTTIID
                     GAGNSKDIEARVQQIRVQIEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHSTRAAVEEGVVPGGGVALIRALKALDGLKGDNHDQDVGINIARAAMEMP
                     LRQIVENAGEEGSVIVNKVKEHSGSVNYGYNAATGTYGDMLEMGILDPAKVTRTALQA
                     AASIAGLMITTECMVTEFPEDKAAPAMPDMGGMGGMM"
     gene            5763..6113
                     /gene="arsC"
                     /locus_tag="PKI23_05990"
     CDS             5763..6113
                     /gene="arsC"
                     /locus_tag="PKI23_05990"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766642.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNN36523.1"
                     /translation="MSEITLYHNPRCSKSRQALQLLNERGITPTILLYLENPPDLPTL
                     RRLLQQLNLPARALLRSGEAEYRALGLDDPACDEETIIAAMAATPKLIERPILVVDSR
                     AVIGRPAEKVLELL"
     gene            6117..6740
                     /gene="wrbA"
                     /locus_tag="PKI23_05995"
     CDS             6117..6740
                     /gene="wrbA"
                     /locus_tag="PKI23_05995"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNN36524.1"
                     /translation="MVTSANPYVLVLYYSRHGATRNMALQIARGVEQVEGIEARLRTV
                     PAVSPVNQAVEPAIPDEGAIYCEEADLRDCAALALGSPTRFGNMAAPLKYFLDGTGAL
                     WGSATLADRPAGVFTSTSSLHGGQESTLLSMMLPLLHHGMILTGIPYREAALHRTRSG
                     GTPYGPSHHAGDGQAALSEDEKVLCQAFGKRLASLALTLHHARRGAH"
     gene            6741..7148
                     /locus_tag="PKI23_06000"
     CDS             6741..7148
                     /locus_tag="PKI23_06000"
                     /inference="COORDINATES: protein motif:HMM:NF021369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNN36525.1"
                     /translation="MAAPPLSRTAGCGNRSRIAQQTALCCLLALLALFALQTLWLDPP
                     TALPPAVALAIQALPLLAFLPSAIKGSPRSMVWLCFVLLLYFIVAVPHLFLPAMLLFG
                     ILEVTLITLLFVAAMLFVRWHRHERTAADQEDD"
     gene            complement(7201..7440)
                     /locus_tag="PKI23_06005"
     CDS             complement(7201..7440)
                     /locus_tag="PKI23_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNN36526.1"
                     /translation="MSEAVIAQKAPYGVAVEAGKTYYWCSCGKSAKQPFCDGSHKEGP
                     FTPLAYEAAESKTVYFCGCKRSAAGALCDGTHKNL"
     gene            complement(7475..8347)
                     /locus_tag="PKI23_06010"
     CDS             complement(7475..8347)
                     /locus_tag="PKI23_06010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNN36527.1"
                     /translation="MASIMLIEEVAPLPQAWLSGLHAANHRLLRSSMDAAPQQVDLLI
                     VWAADPARLQTFLTEHAQAATLAVIGDGVDATPWCASGVDDLAQISLEQPLFQHRVEL
                     LLRLQAQKRALQEAQAQLVRLAVTDPVTGVFNRAHFLSLAHNELSRSLRFSVPFSLIM
                     LDIDQFKERVARQGEEVANRLLVHCAGICQAEVRQVDILGRMSGSEFGICCPETPLAG
                     ARTIAERIRSAMTRSMQSIDESLRFTISGGLATLEELEMDLGSVLGRADRALAEAQSR
                     GGNRMVAGSRKSAL"
     gene            complement(8347..9549)
                     /locus_tag="PKI23_06015"
     CDS             complement(8347..9549)
                     /locus_tag="PKI23_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNN36528.1"
                     /translation="MSEDRFEQIRPYRDDEVRPVLERLLRDPEFLDLFGNLMLPRLHR
                     FVPVLTRWLVRRAFRKEVAGIDSVARLQDRVEEILSRVLQQTSEGFEAIGVENIPHDQ
                     GCLFISNHRDIVLDPALINYALYHQGLQTVHCAIGDNLLSKPFAADLMRLNKSFIVKR
                     SAKGAKELLRNSQLLSDYIRDRLEHSANVWIAQSEGRAKDGMDRTDPAVIKMLLLAYR
                     RDALELTEAVRRMNLIPVAISYEYDPCDERKAVELDQIARLGRYEKAPFEDLTSIAAG
                     VQGQKGRIRLVFGKPLSGTFSDPDQVAAAIDRQIIANYALFPPSWIAWRELQAEGATD
                     LPASHDLQRPDESAEDKRGFLQRCAKLTPGQRSHFLRMYANPLLARQQLDAASVRTDS
                     EQDNRGGG"
     gene            complement(9611..11071)
                     /gene="sbcB"
                     /locus_tag="PKI23_06020"
     CDS             complement(9611..11071)
                     /gene="sbcB"
                     /locus_tag="PKI23_06020"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HNN36529.1"
                     /translation="MNSSAPTFLWHDYETFGLDMRIDRPAQFAAVRTDLALQPVAEPV
                     VLHCRLSDDCLPQPRSCLITGITPQRCDELGVDEARFARALQSELASANSCIVGYNNL
                     RFDDGFTRHLFYRNFLDPYAHEWQQGNSRWDLIDLVRMTYALRPDGIDWPLHEDGTPS
                     FRLEDLTRANQIAHADAHDALADVEATLAVARLIKARQPRLFDYSYRMRDKREVARLI
                     DLRQPTPVLHVSGRFPAAHGHTAIVLPLARHATNANAVICFDLSEDPAPLLECSVEEI
                     RQRLFSSREQLEQQGQRRIPLKLIHLNRCPAIAPLKLLTPQLAERLQIDRARCERHWQ
                     NFPAVAALTDKLQQIFGEPPAQGEPDVEQALYTGSFWSEPDRRLMQQLQQEEPTRLAS
                     YAGRFADPRLTELLFRYRARNHPHTLSESEQTRWQQWRRQRLLEGGPGLLTLAQFFAQ
                     IDQLAVSGEIRSERDRAVLVQLRQHGERLARMVEAA"
     gene            complement(11093..12289)
                     /gene="lpxB"
                     /locus_tag="PKI23_06025"
     CDS             complement(11093..12289)
                     /gene="lpxB"
                     /locus_tag="PKI23_06025"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNN36530.1"
                     /translation="MTMSPRIALVAGEVSGDLLGGPLMAALRQRFAHASFEGIGGERM
                     LAQGLSPLAEMERLSVMGLVEVLGRLPELLALRHRLVSHWIDDPPDLFIGIDAPDFNL
                     GLEARLHAAGIRTAHYVSPSVWAWRQGRVKKIGKAVDLMLTLFPFEADFYRKADVAVE
                     FVGHPLADELIEFGDAAAHRAAFDLSAEVPLVALLPGSRAGEVSRLAPLFFDTAAWCL
                     RRLPALRFILPCANTARRAQLEQLLAQRDDSLPISLVDGRSREAMAAADAVLVASGTA
                     TLEAMLIGRPMVIAYRLAPLSFWILSKLVKVPHVGLPNLLLHEQIVPEFLQNAAQPEL
                     LGQELLRLLESDSDREPMLVSFAQVRQQLARDASQRAAEAVSRLLPTSGSDHPIRQSQ
                     DRVPAG"
     gene            complement(12370..13140)
                     /gene="lpxA"
                     /locus_tag="PKI23_06030"
     CDS             complement(12370..13140)
                     /gene="lpxA"
                     /locus_tag="PKI23_06030"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNN36531.1"
                     /translation="MIDPRAIIDKDARLASDVVVGPWSIIGPDVEIGAGTVIGPHVVI
                     ARSTRIGCNNRIYQFATIGEDCQDKKYHGETTYLEIGDHNIIREGCTLHRGTVQDKSL
                     TRIGNHNLLMAYVHIAHDCIVGDHTVFANNATLGGHVQVADWAILGGYTLVHQFCQIG
                     GHAFCGTGSVVLKDIPAYVMVNGNSAKAHGINSDGLRRRNFSDEQINTIRRAYRILYR
                     EGRSAAEAIEQIAVLVDESPVLLPLLESLRRSTRGIVR"
     gene            complement(13133..13579)
                     /gene="fabZ"
                     /locus_tag="PKI23_06035"
     CDS             complement(13133..13579)
                     /gene="fabZ"
                     /locus_tag="PKI23_06035"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNN36532.1"
                     /translation="MIDVNEIKEYLPQRYPFLLVDRVTDLIVGESINAYKNVTVNEHF
                     FEGHFASHPVMPGVLVVEALAQAAGILGFKTMNQKSSSDSIYYFAGIDDVRFKRPVVP
                     GDRLDLFAKVISSKKGVWKFECRASVGDQLVCEGTILCAHRKLTRD"
     gene            complement(13619..14644)
                     /gene="lpxD"
                     /locus_tag="PKI23_06040"
                     /pseudo
     CDS             complement(13619..14644)
                     /gene="lpxD"
                     /locus_tag="PKI23_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
     gene            complement(14652..15155)
                     /locus_tag="PKI23_06045"
     CDS             complement(14652..15155)
                     /locus_tag="PKI23_06045"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNN36533.1"
                     /translation="MKKLQIWSMVGLMGLAFSAVAELKVAVLDVQTAVLSSDEAKKFI
                     EQVKKETGPDEEKLKGISEEGRKLQERMQKDGPIMSEDEKRKLAEKIEEKAVNYKYLV
                     GKLQSTQRDKQQELLSKMNPKVEKAVTEIIKAEQFDLVLQRQAVVYAVSALDITAKVT
                     DRINQQK"
     gene            complement(15212..17527)
                     /gene="bamA"
                     /locus_tag="PKI23_06050"
     CDS             complement(15212..17527)
                     /gene="bamA"
                     /locus_tag="PKI23_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNN36534.1"
                     /translation="MKALCKLFVLLGGLTWFCAHAAESAFRVTDIRVDGLKRVSAGTV
                     FSALSVNVGETIDQGRLDQVVHELFETGYFQDISIGREGGVLIIKVVERPAISKIEIS
                     GNKAIKTEQLTKAMKGVGLAEGQVFRRAVLDRMTLELKRQYVSEGRYGAEIKSEITEE
                     ARNRVAIQIAIEEGEVAKVRHINIVGNEIFSDKELLKLFEIDTGSLFSLFTKSDQYSR
                     EKLSGDLERLRSYYLDRGFIQFEIDSTQVSVSPDKEEVYVSINVREGAEFKVRNVRLA
                     GDLKVLESELRSLITTQSGEVFSRQKMTASADLLRKRLGDDGYTTANVNGNPEVDTKS
                     NQVDIVYYVEPGNLVYVNRISFAGNSKTKDEVLRRELQQMESSLASTRKIENSKSRLE
                     RLGFFKKVDVETVPVTGATDKIDLKYTVEEQPSGAVTASVGYGQTSGIVYGIGVQQSN
                     FLGTGNNVSFNVSKSDYQDLYSLKFTDPYFTVDGVSLGYRFFYQTTDFDEFNVAKYST
                     DAYGAGINFGYPIADTQRLNFGFDVEHTKIKEGVYPEDEIVNYLNEFGDTFDNYLFSL
                     GWRQSTLEGGVLPRRGYATSAVMEVTTPGSTLDYYILRLKSERFFPIRNDWVFHLDGY
                     IGYGDSFNGDGSFPFFKHFYAGGLGSVRGYKDNTLGPKATPNINDPDQSPDAFGGNLL
                     TTLRSEVLFPLPFVDDKASVRSAFFVDAGNVFDTNRDYDFDFAEIRYATGVGLTWVTG
                     LAPLSFSIAKALNPGDGDKTLFFQFSLGYVN"
     gene            complement(17593..>18720)
                     /gene="rseP"
                     /locus_tag="PKI23_06055"
     CDS             complement(17593..>18720)
                     /gene="rseP"
                     /locus_tag="PKI23_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNN36535.1"
                     /translation="LPDQIHILNVAFLGKPVFARIAIVAAGPIANLLLAWLVYWAVFV
                     LGVENVKPVIGEVTAGSLAERAGVQSGSEIIAVDEAITLTWQEVSIRLLSRLGESGTL
                     QLTVTPSKSNIHEVVRIPLDNWLRGTEDPDPLAALGISPYRPAIPAVLGEVAPDGAAA
                     AAGLRSADQIVAVDGEPISDWQQWVERVRQHPGQAMEVLFRREGVDLKLLVTPQERKD
                     EAGRAIGYLGVGAPPVVWPPELLREIRHPWWSAWLPAAEKVGAMMLLTLDSIQKMVTG
                     LISVDQIGGPITIAKAAGSSLASGATALLGFIAYFSISLGILNLLPIPLLDGGHLLYF
                     LVEAVRGKALSEHAQMIGLRIGLAILAALMLLALYNDFRQL"
BASE COUNT         3920 a         5954 c         5295 g         3551 t
ORIGIN      
        1 ggccgcgatc atcggtgaca aggtgcgggt caccggcaag aagcgcgacg acctgcagca
       61 ggccatcgcg ctgctgcgcg ccgccgagtt cgagctgccg ctgcagttcg acaacttccg
      121 cgactgagcg ccgcgctcaa tccttgcccc agcggaacag cagcgcgccc agcgtcagaa
      181 acagcagcgt catccccccc agcacggtga catggtgcat cacctcgctg agggtggcgc
      241 catcgaccat gatcgcgcgc gcggcggtca ccaggtgggt cagcggaaac agctcggcca
      301 gccacttcag cactggatgg gcgccatcga gcgagaacca gacttccgag agaaacatca
      361 tcggccaggt ggcaaagttg agcagtccgc tggccagctc ctcgctctcg gtgcggctcg
      421 ccaccagcaa gcccagcgcg atcatgcaca acccgccgag ggccgcgacc agcagcagtg
      481 tgagccggct gccgagcatc agaaaatcga gaaacagcgc gcaaccagca tagatgatca
      541 cggcgatgat cagcagaatc gacagccgtg acagcacctg ggcgcagatg aattgcagcg
      601 gcgtaaccgg ggtggcctgc aaccgcttga gcacgccatt cttgcggtaa cgcacgatca
      661 catagccgac gccaaagagc gcaccgaaca tcatgttcat gctgatgatg cccggcatca
      721 cccagtccag atagcgcact tcgcgccctt ggaccacctc cttgaccagc gctcttttct
      781 cctcctgccg cagcagtcgc tcgacgaagt agcccttgcc ggaggtttca ttgacccagt
      841 aacgcaccgc atcgccctcg gccaccaaca ggtcgatctg atggtgctgc acccgggaga
      901 tcgccttgtc gaggttgtcg aacggcacga tctggatgtg tttcagctgt gccagcgcgg
      961 tgcggtcggc accgccggcg ggcgcataga cccccacctt gaacagatcc tgacgcggat
     1021 cagagaaggc aaagacgaaa ccgaccacca gcatcagcgg cagcagcagg ttccaggtca
     1081 gggaggcgcg atcgcggtag aactccgcat tgcgcgcccg cagaagaatc agaaattttt
     1141 tcatgtaacc ccttgttttt attcccgtaa ctgatgcccg gtcagcttga tgaagagatc
     1201 ttccagcgtg ggattgcgca cccgcaacga gtcgagcggc acaccggccg caatcaatcg
     1261 ggccagcgtt gcctcgaccg actgactgag aatctccact ccttcggccg tcacgctgac
     1321 cggctcgggc agcgcttcga cctccggcgt cagcgtgagg cgatcgagac agacccgcac
     1381 atggccgaag tgccgccgca gcagctcctg gggcgccccc tgctcgatga tgcgaccatg
     1441 gtcgatgatc accagccggt cacacagccg ctcggcctcc tccatgtagt gggtcgtcag
     1501 cacgacggtc ttgccccgtg ccttgatgtc gttgatcaga tcccagaagc ggcggcgcga
     1561 ctgcggatcg agcccggtgg tcggctcgtc gagaaagacg atctccggat cgtgaatcag
     1621 cgccagcccc agcagcaacc gctgcttctg tccgccggag agccgggtgg cgtactggtt
     1681 tttgaactcg ccgaggtcgc acatttccac caccgcatcg accggcagcg cgcgcggata
     1741 aaagctggcg aacagccgca aaatttcttc gacagtgatg aaatccatca gcgcggtcga
     1801 ttgaaactga atgccggcct cctgcttgaa gtggcgggtg cgcggctggc cacggtagag
     1861 aatctcgccc gaggtgggag ttttgatgcc ctcgatcatc tcgatggtgg tggtcttgcc
     1921 agccccattc ggacccagca gaccaaagca gcagcctgcc tcgatcgcaa aggagatgcc
     1981 attgaccgcc ttcaccttgg ggtagacctt gaccagctcg cgcacttcga gaatcggcgt
     2041 catggcaatg aagtttccac gttgacaggt tgagacgcag gcgttgtcag ccacccttcc
     2101 agttgcttca gcagccagaa ccccggcacg gccagcagcg tacagaggat gaaaaagccg
     2161 ctccagccca gcagttcagc catccagccg gtgggggctg ccagcagcgt gcgcggaatg
     2221 cccatgatgc tgctgaagag cgcgtactgg gtggcggtgt aacgctgatt ggtctggctg
     2281 gccatgaaag ccaaaaaggc cgcagtgccc atgccggccg tcaggttctc ggtcgccacc
     2341 accagcgcca gcccccccgg actgtgaccc agccgtgtca gcagcacgaa gccgagggtg
     2401 gagagcatct gcgcaaagcc gaagtgccac agcgccgtcc gaataccgac ccgcaacacc
     2461 cagacgccgc cgagcagcgc acccagcagt gtggcgctga agccaaacag cttgaccact
     2521 gcaccgatct cggccggact gtaaccaaga tcgacataga acgggctggt catggccgaa
     2581 gccagggtat cgcccaactt gtagaacaga atgaaggcca gcacccacca agcatcggca
     2641 cgcatgaaaa agtccctgaa cggctcgacg accatcgccc gcaacccccc ggactgcaca
     2701 gcgggcgctg ccggctccgg ggccagcagc gtcgtcagca ggccggcacc catcagcagc
     2761 gccatcaccc agtagacagc ggcaaagccg atctgcccgg ccagaatcag gccgcctccc
     2821 gaagcgagca acatgccgat gcggtagcca taggtgtagg cgctggcgcc cagacccagt
     2881 tcggtttcgg gcagatgctc acggcggtag gcgtcgatca gcgtgtcctg ggtcgcactg
     2941 caaaaagcca ccaatagcgc gaccagcgcc agcgcggtca gattgtcctg tggatccatg
     3001 ttgcccagca gtgcaatcgc gaccatcagc gtgctttgcg tccacaacag ccagcctcgt
     3061 ctgcgcccga gcggccatgg gctgtagcgg tcgagcagcg gcgcccagag gaacttgagc
     3121 gtatagggca accccaccag cgcgaacagc ccgatggtgg tgaggtctac cccactgctg
     3181 cgcagccata cctgcaacag cgacagcgtc aacagcagcg gcaacccgga actgaagccc
     3241 atcatcaagg tgatcagcat ccgggcgctc aatagaacgc gccatagatc aaaaaaccag
     3301 aagcggctgt tcacgaattc ggcagtcagc tgtgggtggg gcagtcggcg attctactgg
     3361 cgagcaggct tgggcgtcca acccgcctgc ttgcgcggcg accacggcac gatggggctc
     3421 gaaggggttg aaaagccgtt ctccgccccc agaaagtgga cgcgctggaa tgaagaggcg
     3481 ggctgcacgc cagcttgact ctcttctctt cgcccctatc attggcactc cccaggtcag
     3541 agtgccaatg ataggggcga agcagccccg ataaatctga ccaacaacat tgcataaagt
     3601 ggagagtgac cgcaatgaag attcgtccgt tacacgatcg tgttgtcgtg cgccgcatgg
     3661 aagaagagac caaaaccgct ggcgggatcg tgctgcccgg tagcgcggcc gaaaagccct
     3721 cacaaggtga agtgatcgcc gtcggcaagg gcaaggccct cgacaatggc gagattcgtc
     3781 caatggatgt gaaggtcggc gatcgcatcc tgttcggcca atacagcggc agcaccgtca
     3841 agatcgatgg caaggagctg ctggtgatga gcgagagcga aatcttcgcc gtgctcgaat
     3901 cctgatccgt tgctccaatt cgttcatcaa ccttttccat aaagagttca aagggaaaac
     3961 acaatggcag ctaaagacgt actcttcggc gacaacgccc gcaaaaagat gctcaacggc
     4021 gtcaacgtcc tggccgatgc ggtcaaggtc accttgggcc ccaagggccg caacgtcgtg
     4081 ctcgacaaat cgttcggcgc accgaccatc accaaggatg gcgtctcggt cgccaaggag
     4141 atcgaactga aagacaagtt cgagaacatg ggcgcccaga tggtcaagga agtggcttcg
     4201 cgcacttccg acgaagccgg tgacggcacc accaccgcca ccgtactggc ccaggccatc
     4261 gtgcgtgaag gcctcaaggc cgacgccgcc ggcatgaacc cgatggacct caagcgcggc
     4321 atcgacaaag ccgttgccaa ggcggtcgaa gccgtcaagc agatggcgca gccctgcacc
     4381 gatgacaagg ccattgccca ggtgggcacc atctccgcca acagcgacag ctcgatcggc
     4441 gagatcattg ccgaagcgat gaaaaaggtc ggcaaagagg gcgtgatcac ggttgaagag
     4501 ggcagcggcc tggaaaacag cctcgacgtg gtcgaaggga tgcagttcga ccgcggctac
     4561 ctctcgccct acttcatcaa caaccaggag agcatgtccg ccgagctgga caacccctac
     4621 atcctgctcc acgacaaaaa aatctcctcg atccgcgaca tgctgccggt tctcgaagcg
     4681 gtcgccaaat cgggccgccc gctgctgatc atctgtgaag atgtcgaagg cgaagcgctg
     4741 gccacgctgg tggtcaacac catgcgcggc atcgtcaagg tctgtgccgt gaaggccccc
     4801 ggcttcggtg atcgtcgcaa ggccatgctg gaagacatcg ccattctcac cggtggcacc
     4861 gtcatctcag aagagctggg cctggcgctc gaaggcacca ccctgcaaga cctcggtcag
     4921 gccaagcggg tcaacatcag caaggagaac accaccatca tcgatggcgc cggcaactcc
     4981 aaggacatcg aggcgcgcgt tcagcagatt cgcgtgcaga tcgaggagac cacctccgac
     5041 tacgaccgcg agaagctgca ggagcgggtc gccaagctgg ccggcggtgt cgccgtcatc
     5101 aaggttggcg cggccaccga agtcgaaatg aaagagaaaa aggcccgcgt cgaagatgcc
     5161 ctgcactcca cccgtgctgc cgtcgaagag ggcgtggtcc ccggcggtgg cgtggcgctg
     5221 atccgtgccc tcaaggcgct ggatggactc aaaggcgaca accacgacca ggatgtcggc
     5281 atcaacatcg cgcgtgccgc catggaaatg ccgctgcgtc agatcgttga aaacgctggc
     5341 gaagagggtt ccgtgatcgt caacaaggtc aaggagcaca gcggaagcgt caactacggc
     5401 tacaacgccg ccactggcac ctacggcgac atgctcgaaa tgggcatcct cgaccccgcc
     5461 aaggtgaccc gtaccgcgct gcaggctgcg gcctcgatcg ccggcttgat gatcaccacc
     5521 gagtgcatgg tcaccgagtt ccctgaagac aaagcggcgc ctgccatgcc cgacatgggt
     5581 ggcatgggcg gcatgatgta acagttcgaa ccgccacaag caactcggac tccgaagagg
     5641 ccctgccacc gcagggcctc ttcttttgaa tgaggcaatc ccgcccctct cccttggaaa
     5701 tcctctgggc aggggtagaa ttctccggta tcaaaaacct cttcatgcca agtactctgc
     5761 ccatgtccga gatcaccctc taccacaatc cccgctgctc gaaatcgcga caggcattgc
     5821 agctgctcaa tgagcgcggc atcacgccga ccatcctgct ctatctggaa aatccacccg
     5881 atctccctac gctgcgccgc ctgctccagc aactgaatct gccggcccgt gcactgctgc
     5941 gcagcggcga ggcagagtac cgggccctcg ggctcgacga cccggcctgc gatgaagaga
     6001 cgatcattgc cgccatggcg gcaacgccga aactgatcga acggccgatc ctggtggtgg
     6061 acagtcgcgc agtgattggc cgccccgccg aaaaagtact ggagctgctc tgagccatgg
     6121 tcacctctgc caatccctac gtgctggtgc tctactacag ccgccatggc gcaacccgca
     6181 acatggccct gcagatcgcc cgcggtgtcg aacaggtgga gggcatcgaa gcccgcctcc
     6241 gcacggtgcc ggcggtgtca ccggtcaatc aggcagtgga gccggccatt cctgacgaag
     6301 gggccatcta ctgcgaagag gccgacctgc gtgactgcgc cgccctggca ctcggcagtc
     6361 cgacccgctt cggcaacatg gccgcgccac tcaaatattt tctcgacggc accggcgcgc
     6421 tgtggggctc ggccacactc gccgacaggc cggccggggt cttcacttcc acctcgagcc
     6481 tgcatggtgg acaggagagc acactgctgt cgatgatgct gccactgctg caccacggca
     6541 tgattctgac cggcattccc taccgcgagg ctgccctgca tcgcactcgc agcggcggca
     6601 cgccttatgg ccccagccat cacgccggcg atggtcaggc ggcgctgtcc gaagatgaaa
     6661 aggtgctctg tcaggcattt ggcaaacggt tggccagcct ggcgctcacc ctgcaccacg
     6721 cgcggcgcgg cgcacactga atggccgcac cacccctttc caggactgca ggttgcggca
     6781 accgctcacg aatcgcgcaa cagaccgccc tgtgctgcct gctggcactg ctcgcactct
     6841 ttgcattgca gaccctctgg ctcgatccgc ccaccgccct gccccccgca gtggcactgg
     6901 cgattcaagc cttgccgctg ctggcctttc tacccagtgc catcaagggt tcaccacgca
     6961 gcatggtctg gctctgcttt gtgctgctgc tctatttcat cgtggccgtg ccccacctct
     7021 ttttgccggc aatgctgctg ttcggcatcc tcgaagtgac gctgatcacg ctgctgtttg
     7081 tggcggccat gctctttgtg cgctggcaca ggcacgagcg taccgcagcg gaccaggaag
     7141 atgattaggc agaaaaaagc ggcgaccgga gggtcaccgc ccgtggacag aggaggcagt
     7201 tcagagattc ttgtgggtgc cgtcgcacag cgcaccggcg gctgagcgct tgcaaccgca
     7261 gaaatagacc gtcttgctct cggcagcctc ataggccaac ggcgtgaaag gcccctcctt
     7321 gtgcgaacca tcgcagaagg gctgcttggc actcttgccg caggagcacc agtagtaggt
     7381 ctttcctgcc tcgaccgcca caccataggg cgctttctgg gcaatcactg cttcggacat
     7441 gtcgttctcc tcgaggttgt cgttgtctga aagttcacaa tgcgctcttg cgcgaacccg
     7501 ccaccatgcg gttgccaccg cgtgactgtg cttcggccag tgcccggtca gcccggccca
     7561 gtacgctgcc gagatccatc tccagctctt cgagcgtcgc cagaccgcca ctgatggtga
     7621 acctgagcga ctcatcgatc gactgcatcg accgcgtcat ggcactgcga atccgctcgg
     7681 caatggttct ggcgcccgcc aatggggttt cggggcaaca gatgccgaac tcgctgccac
     7741 tcatccttcc caggatgtcc acttgacgca cttcggcctg acagattccg gcgcagtgca
     7801 ccagcagccg attggcgacc tcttcgcctt gcctcgccac ccgctccttg aactggtcga
     7861 tgtcgagcat gatcagcgaa aagggcaccg agaagcgcaa tgagcgcgac agttcattgt
     7921 gcgccagcga gaggaagtga gcgcggttga agacgccggt caccggatcg gtcactgcca
     7981 ggcgtacaag ctgggcctgc gcctcttgca gcgccctttt ctgtgcctga agtcgcagca
     8041 gcagctcgac ccgatgctgg aacaacggct gctccagcga tatctgcgcc agatcgtcga
     8101 cgccggaggc gcaccaaggc gtggcgtcga cgccatcgcc gatcaccgcc agagtggcag
     8161 cctgcgcatg ctctgtcaga aaagtctgca atcgggcggg gtcggcagcc catacgatca
     8221 gcagatccac ctgttgcggc gctgcatcca tgctcgatcg caacagccga tgattggcag
     8281 catggaggcc actgagccac gcctgcggca gcggagccac ctcttcgatc agcatgatgc
     8341 ttgccatcaa ccaccccctc tgttgtcctg ttcactgtcc gttcgtacgc tggctgcatc
     8401 gagctgctgt cgcgccagca gcggattggc gtacatgcgc aagaaatggc tgcgctggcc
     8461 gggtgtcagc ttggcgcagc gctgcagaaa acctcttttg tcttccgccg actcatcggg
     8521 ccgctgcaag tcatgcgaag caggcaggtc ggttgccccc tccgcctgca attcgcgcca
     8581 ggcaatccag cttggcggaa agagcgcgta gttggcgatg atctgccgat ctatcgccgc
     8641 cgccacctga tcaggatcgc tgaacgtccc actcagtggt ttgccgaaga ccagccgaat
     8701 gcgccccttt tgcccctgga cgcccgcggc gatgctggtc agatcctcga acggcgcctt
     8761 ttcgtaccgg cccaggcgcg caatctggtc gagttccacc gccttgcgct catcgcaggg
     8821 gtcatactcg taagagatcg ccactgggat cagattcatc cgccgcaccg cttctgtcaa
     8881 ctccagggca tcgcgtcgat aggccagcag cagcatcttg atcaccgctg gatcggtgcg
     8941 gtccatgcca tccttggccc gcccctcact ctgcgcaatc cagacattgg cgctgtgctc
     9001 gaggcggtcg cgaatgtaat cggagagcag ctggctgttt ctcagcaact ccttggcgcc
     9061 cttggcgctg cgcttgacga tgaagctctt gttcaggcgc atcagatcgg ccgcgaaggg
     9121 cttgctcagc aggttgtcac caatggcgca atgcaccgtc tgcaacccct gatggtagag
     9181 cgcataattg atcagtgccg gatcgagcac gatgtcgcgg tggttggaga taaacaggca
     9241 gccctgatcg tgcgggatgt tttcgacgcc gatcgcttca aacccctcgg aggtctgctg
     9301 gagaaccctg gaaagaatct cctcgacccg atcctgcagc cgtgccactg agtcgatccc
     9361 cgcgacctct tttctgaaag cacgtcgaac cagccagcgt gtcagcaccg gaacgaatcg
     9421 gtgcagccgt ggcagcatca ggttgccgaa cagatcaagg aattccggat cgcgcagcag
     9481 ccgttcgaga acaggccgta cctcatcatc ccgatagggt cgaatctgtt caaaccgatc
     9541 ttcactcatt gcttctgctc cacggttggc atgattgaat tcccggggac accacccttc
     9601 ttcgccggtc tcacgccgcc tccaccatcc gcgccagccg ctcgccatgc tgacgcaact
     9661 gcaccagcac cgcccgatcg cgttccgaac ggatctcgcc actgaccgca agctgatcga
     9721 tctgcgcaaa aaactgggcc agcgtgagca gacccgggcc gccctccagc agccgctgac
     9781 gccgccactg ctgccagcgc gtctgctcgg attcgctcag agtgtgcgga tggttgcgcg
     9841 cccggtagcg aaacagcagt tcggtcagac gcggatcggc aaagcgcccg gcatagctgg
     9901 ccagtcgtgt cggctcctcc tgttgcagct gctgcatcag gcgccggtcc ggttcgctcc
     9961 agaagctgcc ggtataaagc gcctgctcga catcgggttc accctgtgcg ggaggctcgc
    10021 caaaaatctg ttgcagcttg tcggtcagcg cggccactgc cggaaagttt tgccaatgtc
    10081 gttcacagcg ggcgcggtcg atctgcaacc gttctgccag ttgaggcgtc aacaacttca
    10141 gcggtgcaat ggccggacaa cggttgagat ggatcagctt gagcggaatg cgccgctgtc
    10201 cctgctgctc caactgctcc cggctggaga agagccgctg acggatctcc tccacgctgc
    10261 actccagcag tggcgcggga tcttcgctca gatcgaaaca gatcaccgca ttggcattgg
    10321 tggcgtgacg cgccagtggc agcacgatgg cggtgtggcc atgcgctgcc ggaaaacggc
    10381 cagagacatg cagtacggga gtcggttggc gcaggtcgat caggcgggcc acctcgcgct
    10441 tgtcacgcat ccggtagctg tagtcgaaca gccgcggctg ccgcgccttg atcaggcggg
    10501 ccacggcaag ggtggcctcg acatcggcca aggcatcgtg cgcatctgca tgggctatct
    10561 ggttggcacg ggtgagatct tcgaggcgaa agctgggtgt gccatcctca tgcagtggcc
    10621 agtcgatgcc gtcgggacgc agcgcatagg tcatccgcac cagatcgatc agatcccagc
    10681 gactgttgcc ctgctgccat tcatgcgcat aggggtcgag aaaatttcga tagaacagat
    10741 ggcgggtgaa gccatcatcg aaccgcaggt tgttgtagcc gacgatgcag ctgttggcac
    10801 tcgccagttc gctctgcaga gcgcgcgcga atcgggcttc gtcgacaccc agctcatcac
    10861 agcgctgcgg cgtgatccca gtaatcaggc aggagcgtgg ctgtggcaga cagtcatcgc
    10921 tcagtcggca gtggagcacg accggctctg ccaccggctg caatgcaagg tcggtacgca
    10981 ctgcggcaaa ctgcgccggc cggtcgatgc gcatgtcgag accaaaagtt tcgtaatcgt
    11041 gccacaagaa ggttggagca ctgctgttca tcctgcctcc agagacatcc gctcatcccg
    11101 ctggaacgcg gtcttgcgac tgccggatgg ggtgatcgct gccggatgtc ggcagcagcc
    11161 gggaaactgc ctcggccgcc cgctggctgg cgtcgcgtgc caactgctga cgcacctgcg
    11221 caaagctgac caacatcggt tcacgatcgg aatcggactc cagcagccgc agcaactcct
    11281 ggcccaacag ttccggctgc gccgcattct gcaggaattc aggcacgatc tgctcatgca
    11341 gcagcagatt gggcagcccg acatggggca ccttgaccag tttcgagagg atccagaaac
    11401 tcaatggcgc aagccggtag gcaatcacca tcgggcggcc gatcagcatc gcctccaggg
    11461 tcgcggtgcc cgaagccacc agcacggcat ctgccgccgc catcgcctcg cgcgaccgtc
    11521 catccaccag gctgatcggc agagagtcat cacgctgcgc cagaagctgc tccagttgcg
    11581 cccgacgcgc cgtattggcg catggcagga tgaaccgcag tgccggcaat cgccgcagac
    11641 accaggcggc ggtgtcaaag aacagcggcg ccaaacggga aacttcgcca gcacgactgc
    11701 cgggcagcag cgccaccaag ggcacttccg ccgagagatc gaatgccgca cgatgcgctg
    11761 ccgcatcgcc aaactctatc agctcatcgg ccaggggatg gccaacgaat tcgaccgcca
    11821 cgtcggcctt gcgataaaaa tcggcctcga acggaaagag cgtcagcatc agatcgacgg
    11881 ccttgccaat cttcttgacc cgtccctgcc gccaggccca caccgaagga ctgacataat
    11941 gggcggtgcg gatgcccgct gcgtgcagcc gggcctccag tccgaggttg aagtccgggg
    12001 catcgatgcc gatgaagaga tccggcggat cgtcgatcca gtggctgacc aggcggtgac
    12061 gcagcgccag cagttccggc aaccgcccca gcacctcgac cagccccatc accgacaacc
    12121 tttccatctc ggccagtgga ctcagccctt gggccaacat ccgctctccg ccaatgcctt
    12181 caaaactggc atgcgcaaac cgctgcctca aggcggccat caacggcccc ccgagcagat
    12241 ctcccgagac ctctccggca accagagcaa tacgcggtga catggtcatt gagcctgctg
    12301 ggctggcact gtctgttatt tttgtctcat gcggcgccgg gtggcttgac cgcgaatctg
    12361 ccttggccgt cagcgaacga tgccacgcgt cgaacggcgc agtgattcga gcagcggcag
    12421 caggaccgga gactcatcca ccagcactgc gatctgctcg atcgcctcgg cggcactgcg
    12481 accttcgcgg tacaggattc ggtaggcacg ccgaatggta ttgatctgct catcggagaa
    12541 attccgccgc cgcaacccat cgctgttgat gccatgcgct ttggcgctgt tgccattgac
    12601 catcacatag gcaggaatgt ctttcagcac cacactgccg gtgccgcaga aggcatgccc
    12661 gccaatctga cagaactgat ggaccagtgt atagccaccc agaatggccc agtcggccac
    12721 ctgcacgtgg ccacccagcg tggcattgtt ggcaaagacc gtgtggtctc caacgatgca
    12781 gtcatgcgca atgtgcacat aggccatcag cagattgtgg ttaccgatgc gggtcaacga
    12841 cttgtcctgc accgtgcccc gatgcagtgt gcacccctca cgaatgatgt tgtggtcacc
    12901 gatctcgagg taggtggttt caccgtggta tttcttgtcc tggcaatctt cgccaatggt
    12961 ggcgaattga tagattcgat tgttgcagcc gatccgggtc gagcgcgcaa tcaccacatg
    13021 gggaccgatg accgttcccg caccgatctc gacatccgga ccaatgatgc tccagggtcc
    13081 aaccaccaca tcggacgcca gccgtgcatc cttgtcgatg atggcacgcg gatcaatcac
    13141 gggtcagctt ccgatgcgca cagaggatgg tgccttcgca gaccagctgg tcacccaccg
    13201 atgcacggca ttcgaacttc cagacaccct ttttgctgga aatgaccttg gcaaagagat
    13261 cgaggcggtc tccaggcacg acgggacgtt tgaagcgaac atcgtcgata ccggcgaaat
    13321 agtagatcga atcactcgag cttttctgat tcatcgtctt gaagccaaga atcccagccg
    13381 cctgcgccag cgcttcgacc accagaacac ccggcatcac cggatgagag gcgaagtgcc
    13441 cctcgaaaaa atgttcgttc accgtgacat tcttgtaggc attgatcgac tcgccaacga
    13501 tcagatcggt gactcgatcg accagcagaa aggggtagcg ctgcggcagg tactccttga
    13561 tctcattcac atcaatcatt gcttcgtggc tctcaggttg aggcagacag tgattcggtt
    13621 cagcaagatg ctcttccagc cggcgcagac gtcgggccat ctcatcgaga tggcgaaagc
    13681 gcgcaacatt ttttctccac tccagattct cgagcatgcc ggtgccggat gaataggcac
    13741 ctgacttcga aatggagcgc gtcaccatcg ccatgccggt cagatgaaca tcgtccgcga
    13801 tggtgagatg cccaccaatg cccacggcac cgccaatggt gcaacgcctg ccgataacgg
    13861 tactgccaga gattccggtt ccagcagcga tcgccgttcc ctcgccgatc ttcacattgt
    13921 gcgcgacctg caccccgtta tcgagtttga ccccgttctc gatgacggtg tcctcgatcg
    13981 cgccacggtc gatggtggtt ccggccccga tttcgacatc atcgccgatg atgaccgaac
    14041 cgatctgggc aattttatgc caggcattgt cgcttctggc aaaaccgaaa ccgtcggcac
    14101 cgatcaccgc ctgactgtgg atgatcactc tcctgccgag gcgcactccg tggtagagga
    14161 ccacctgcgg atgcaatcga ctgtgggccc cgatcacaca atcctgaccc acgtagcaac
    14221 cactgccaac cacggcatgc gcttcgagca ccgcaccgga ctcgatcacg gcaagggggc
    14281 ctatgcaggc agtcgtggcc acatgcgcct gtggactcac cttggccgag gggtcgatgc
    14341 ccgttgccat gacgggtgcg ctgtcaaacc agtgagaaac cttggcatag gcaaggtagg
    14401 gatcggctac cacgatatgt ggcaagggac actctttcgc cgcttctgca ttcagaatga
    14461 ccgcacccgc gtcgctggtg gccagatatt ttcggtaacc cggattggac agaaaagcca
    14521 attgatctgc ccgagcacgc tgcagggtgg ccagtccggt gatctccaac tgcggatcac
    14581 ccgacagcac cccgcccagt cgttcggcaa tctcgcccag tcgatagcgt ggttttttga
    14641 ccatgcccgg atcacttctg ctgattgatc cgatcggtca ccttggcggt gatgtccagt
    14701 gcggagaccg cataaaccac ggcctgtcgt tgcagtacca gatcgaactg ctcagccttg
    14761 atgatctccg tgaccgcttt ttcaaccttc gggttcattt ttgaaagcag ctcctgctgc
    14821 ttgtctcgtt gcgtactttg cagtttgccg accagatact tgtagttgac cgctttttct
    14881 tcgatttttt ctgccagttt ccgtttttca tcctctgaca tgatcggccc gtctttctgc
    14941 atccgttcct gaagctttct gccctcttcg gagatgcctt tcaatttctc ttcatccggc
    15001 ccggtctctt ttttgacctg ctcgatgaat ttcttcgcct cgtcggagga gagcacggcg
    15061 gtttgcacgt caagaaccgc aactttcagc tctgccactg ccgaaaacgc cagccccatc
    15121 agcccaacca tcgaccatat ctgcaatttt ttcatgaaaa aactccggtt gattgtcaaa
    15181 tattgcaagg aatatcaaaa cagtattcag atcagttgac ataacccagt gagaactgga
    15241 agaacaaggt cttgtctcca tcacccggat tcagcgcttt ggcaatgctg aaagagagtg
    15301 gtgccaaccc ggtcacccat gtcaatccta cacccgtggc gtatctgatc tcggcaaaat
    15361 caaaatcgta atccctattg gtgtcgaaga cattgcccgc gtcgacaaag aacgcactgc
    15421 gcaccgaggc cttgtcatcg acgaaaggaa gcgggaacag cacctcactt ctgagcgtcg
    15481 tcagcaggtt gccgccgaag gcatctgggc tctggtcagg atcattgatg tttggcgtgg
    15541 ccttcggtcc aagcgtgttg tccttgtaac ctcggaccga tccgagtcca cctgcataaa
    15601 agtgcttgaa gaacgggaat gaaccgtcac cattgaaact gtcgccatag ccaatgtagc
    15661 catccaggtg aaatacccag tcattcctga tcggaaagaa ccgctcactc ttcaacctga
    15721 gaatgtaata gtcaagcgtg ctccccggcg tggtgacctc catcaccgca ctggtggcat
    15781 agcctcttct gggcaaaaca cctccctcaa gtgtcgactg ccgccagccg agggagaaca
    15841 gataattgtc gaaggtatcg ccaaactcat tcagatagtt gacgatctcg tcttctggat
    15901 agaccccttc ctttattttt gtatgctcta catcaaaccc gaaattcagc cgctgcgtgt
    15961 cagcgattgg ataaccgaaa ttgattccgg ccccataggc gtcggtcgaa tattttgcaa
    16021 cattgaactc atcgaaatcc gtggtctgat agaagaaccg atagccgaga ctgacaccat
    16081 cgacggtaaa ataggggtcg gtgaacttca ggctgtagag atcctgatag tcgcttttac
    16141 tgacattgaa actgacattg ttgcccgttc caagaaaatt gctctgctgg actccaatgc
    16201 catagacgat gccggaggtc tgcccataac ctacgctggc agtgacggcg ccggagggct
    16261 gctcttcgac cgtgtacttg aggtcgatct tgtcggtggc gcctgttacc ggaacggttt
    16321 cgacatccac cttcttgaag aacccaaggc gctcaagacg actcttggag ttctcgatct
    16381 tgcgcgtgga ggccagcgaa ctctccattt gttgtagttc acgtcgaagc acttcgtcct
    16441 tggtcttgct gtttcctgcg aaactgattc tgttcacata gaccagattg cccggttcga
    16501 cgtaatagac gatatcaacc tgattgcttt ttgtgtcgac ttccggattg ccattcacat
    16561 tggccgtggt gtagccatca tcacccaatc tcttgcgcag cagatcagcc gaggcggtca
    16621 ttttctgccg tgaaaacact tcaccgctct gcgtggtgat cagcgacctc aactcggact
    16681 ccaaaacctt cagatcgcct gcgagcctga cgtttcgtac tttgaattct gcaccttcac
    16741 ggacgttgat gctgacatag acctcttcct tgtctggcga aaccgaaacc tgcgtcgagt
    16801 cgatctcgaa ctgaatgaaa ccccgatcga gataataaga acgcaggcgc tccagatcac
    16861 ccgagagttt ttctcgggaa tattgatcgg acttggtgaa gaggctgaac agactgccgg
    16921 tgtctatctc gaacaatttc aacagctctt tgtcgctgaa aatttcgttt ccgacaatgt
    16981 tgatatgtct gaccttggcc acttcaccct cttcaatggc gatctgaatg gccacccggt
    17041 tgcgagcctc ttccgtgatt tcgctcttga tctcggcacc ataacgcccc tcggaaacat
    17101 attggcgttt cagctcgagt gtcatccggt cgagcaccgc acgacgaaaa acctgacctt
    17161 cggcaagacc aacccctttc atcgccttgg tcaactgctc ggtcttgatg gccttgttgc
    17221 cactgatctc gatcttgctg atcgctggtc gttcgacgac cttgatgatc aaaacccccc
    17281 cctcgcgacc aatggagatg tcctggaaat agcctgtttc aaacagctca tggaccacct
    17341 gatccagtct gccctgatcg attgtctctc cgacattgac cgagagcgcc gaaaagaccg
    17401 tccctgccga cacccgtttg agtccatcga cgcggatatc ggtgacccga aatgcgctct
    17461 cggccgcgtg ggcgcagaac catgtcagac caccaagcag aacgaacagc ttgcacaacg
    17521 ctttcatagt gatctatcgg attctagtgg tggcaggatt attgatattg ttcgaaaaaa
    17581 gatcggcagc catcacaact gcctgaagtc attatatagt gccagcagca tcagcgcagc
    17641 cagaatcgcc aggccgatgc gcagaccgat catttgggca tgctccgaaa gcgccttgcc
    17701 gcgaaccgcc tcgaccagaa aataaagcag atgcccccca tccagcagag ggatcggcag
    17761 cagattgagg atgccaaggc tgatgctgaa ataggcaatg aaccccagca gggcggtcgc
    17821 gcccgatgcg agcgagcttc ctgccgcctt ggcgatggta atcggcccac cgatctgatc
    17881 gaccgaaatc agtcccgtga ccattttctg gatcgagtcg agcgtcagga gcatcattgc
    17941 gccaactttt tccgccgccg gcaaccatgc actccaccat gggtggcgaa tttctcgcaa
    18001 caactccggc ggccagacaa cgggcggtgc cccgaccccc agatagccga tggctcgtcc
    18061 cgcttcatct tttctttcct gcggcgtcac cagcaacttg agatcgaccc cttcgcgcct
    18121 gaacagcacc tccatcgcct gcccgggatg ctgacgcacc cgttcgaccc actgctgcca
    18181 gtcgctgatc ggttcaccat cgacggcgac gatctggtcg gcgctacgca atcccgctgc
    18241 cgcagccgca ccgtccggtg cgacttcgcc cagcacggcc gggatggccg gacggtaggg
    18301 tgaaattccc aacgcggcaa gcggatcggg atcctctgtt ccacgcagcc aattgtcgag
    18361 cggaatccgc accacctcat ggatgttcga cttggacggc gtgaccgtca gctgcaatgt
    18421 gccactctcg cccagtctgg agagcaggcg gatgctcacc tcctgccacg tcagggtgat
    18481 ggcctcgtca accgcgatga tttcactgcc cgactgcact cctgcccgtt ccgccagcga
    18541 gcccgccgtc acctcaccga tcaccggttt gacattttcg accccgagca cgaaaaccgc
    18601 ccaatagacc agccaggcca gcagcaggtt ggcgatcgga cccgctgcga cgatcgcgat
    18661 gcgcgcgaag accggcttgc cgaggaaggc cacgttcagg atgtggatct ggtcgggcag
//
LOCUS       DAORCQ010000041        18262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1897_length_18262_cov_6.310815, whole genome shotgun sequence.
ACCESSION   DAORCQ010000041 DAORCQ010000000
VERSION     DAORCQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18262
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_18262_cov_6.310815"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKI23_06060"
     CDS             <1..226
                     /locus_tag="PKI23_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNN36536.1"
                     /translation="GNGIGQLQAEDVGFINTDISLNLHRLPQGEWICIDARTLAQPCG
                     IGMVETVLYDHLGPIGRVTQANLANPLYRG"
     gene            231..554
                     /locus_tag="PKI23_06065"
     CDS             231..554
                     /locus_tag="PKI23_06065"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNN36537.1"
                     /translation="MGNPQAPGATMYVVLFRAKIRAPDDDYLQTAARMRELALQQFGC
                     LEFQSMTQGEEEVAISWWPSEAAILAWKQHPEHLLAQRAGRERWYCAYSVQVAAVVRD
                     YQVVC"
     gene            complement(626..1549)
                     /locus_tag="PKI23_06070"
     CDS             complement(626..1549)
                     /locus_tag="PKI23_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNN36538.1"
                     /translation="MPGLPISSVERETGLSKDTLRVWERRYGFPTPERDANGERSYSL
                     QQVRRLTQLKRLLDQGHRPGKLLKLDEEALSALLGTAAPSAMQSTQQPLDEWLQRVRL
                     HDSDALQRDLQREVNRHGLARFVQDIAAPLITHVGAAWSNREIGVFEEHLFSQNLEKL
                     FHSALANLSQPQGRPRVLLTTLAGEEHTLGLLMVEALLALEEACPIQLGAQTPVSEIA
                     RAARLERIDVVCLSFSSAYLPALAQQGLRDLRQQLPTEVELWAGGHGIKAIRRPIEGI
                     ALTPEFDDLHAALRRWRARQDSMAAHSRSGQ"
     gene            1717..3006
                     /locus_tag="PKI23_06075"
     CDS             1717..3006
                     /locus_tag="PKI23_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNN36539.1"
                     /translation="MMQVAVIGSGISGLATAWLLSRRHRVTLFEAEQRLGGHTHTVDL
                     TLDGQTFAVDTGFLVFNHRTYPQLTQLFRQLGVETVASEMSFSFRMDEPELEWAGSSL
                     DTLFGQRANLVRPPFWQMLGEMLRFNRSAARDLPMHGDLTLGDYLRLRGYGRPFIDWY
                     LLPMAAAIWSAPTARILEFSLSSFVAFCNNHGLLQIFDRPRWYTVKGGGREYVQRLAA
                     GIDQIRLGQPVDSLRRRGGLIELGSGDFQGRYDQVVLACHSDQSALLLERDFPQRAQW
                     LRHIPYQHNQVVLHSDPRVLPRRRKLWSAWNYQAVSSAAGAVQSGTGRAVAVHYLINR
                     LQPLPTTTPVIVSLNPVEPIDPGLVHRRFDYSHPLFSFDSAKVQQQITASNGEQGIWL
                     AGAWLGHGFHEDGLASALRVARALDVVSPWEGLADAA"
     gene            2996..3739
                     /locus_tag="PKI23_06080"
     CDS             2996..3739
                     /locus_tag="PKI23_06080"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNN36540.1"
                     /translation="MLPEMAQIGFGGLRHLRLKPVVNRFSYPLFFVRLPMHELTGVAV
                     PGFSIDRFNLLSFHHRDHGEGGDALRWVRDLLQAQGLVADGAIWLTTFPRVLGYVFKP
                     VSFWHCHRADGQLVAVLAEVNNTFGERHVYLLRAGEPAGAFEAQKVFHVSPFFGRQGR
                     YRFGFHVEAGRFCARIDYDDGDGLALATRWWGKLEPLTARSGRRAFWRYPLMTLAVIV
                     RIHLQAVRLLRKRVRLFPKPVPSPIEVTE"
     gene            3736..4950
                     /locus_tag="PKI23_06085"
     CDS             3736..4950
                     /locus_tag="PKI23_06085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNN36541.1"
                     /translation="MSSVDLTAGLQHNKSPWYALPVVRRLHGIEGGVIRLELPNGRTV
                     RLGQGGVEVSLRLRRWSLLRGILTSGDIGFAQGYIDGGWDTADLTALLTLLARNRTAL
                     GHLIHGQWLGRLQHRLRHLRNANSLKGSRRNIAFHYDLGNAFYALWLDRTMTYSSARF
                     DGDWSLDLGEAQQRKYQRVLELLDLPSGQSLLEIGCGWGGFAEQAAGSAGHRVRGLTL
                     SRQQLEYASARIDAAGLAGQCGFALQDYRDEQGQYDGIVSIEMFEAVGEAYWNDYFAA
                     VRRALRPGGRAVVQSILIDDALFERYRNGTDFIQQQIFPGGMLPSAERFRAVAAENGL
                     VVTHQEHFGLDYAETLNRWERNFIARQDEVRALGFDARFMRLWHFYLAYCQAGFNSGS
                     TDVAQFVLQPAL"
     gene            4916..5455
                     /locus_tag="PKI23_06090"
     CDS             4916..5455
                     /locus_tag="PKI23_06090"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNN36542.1"
                     /translation="MSRSSCSSRRSSAALLLWLTVVALPVVAMAPLPELPFVPRQVGA
                     GNLRHFGIQLYEARLWNGDGLWKPDAPYALELIYARSFTAEAIAERSIVEIRRQGIDD
                     ALLLANWQTRLRALFPDVAAGDRLLGLRQPGAGATFYLGERQIGRIDDEQLAAAFFAI
                     WVGEQSSAPGLRRRLLGLP"
     gene            5452..>6451
                     /locus_tag="PKI23_06095"
     CDS             5452..>6451
                     /locus_tag="PKI23_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN36543.1"
                     /translation="MTTPLLPSHQLIAYGALGLPLAFAALPLYVHLVPFYAESIPLAL
                     LGILLLALRFFDAVIDPLLGVLLDRFPHPRGLIALSLPLLGVGWMGLFVPQRPWPPIP
                     WLLTMLTLTTVGYSLATVVHNAWAARLCATAAERARLFASREAFGLAGVVLAASLPTL
                     LAPTLSTGLTRTGPWFLLLLIALGGVTLLGTQRTVLRVEPFARTQWLRPFAQPLFRRF
                     APGYLLSAVASALPASLVFFFIDDVLRMGAWAGLFLALYFIAGAAGLPLWMRLVHRIG
                     LFDAWLLSMVLAIFTFASAFTLGAGDGLGYALICVASGVALGADLALPSALVAEMVD"
     assembly_gap    6452..6551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6552..6851
                     /locus_tag="PKI23_06100"
     CDS             <6552..6851
                     /locus_tag="PKI23_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN36544.1"
                     /translation="ALGADLALPSALVAEMVDVDQGSQPGLYYGLMSFLAKLALALAA
                     GLALPLLDLWGYQPGGEQLWPLSFAYALLPCLLKLAALAWFRPLRRFFATGGVTC"
     gene            6848..7375
                     /locus_tag="PKI23_06105"
     CDS             6848..7375
                     /locus_tag="PKI23_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HNN36545.1"
                     /translation="MKRSLIALCTLLGLSGCAGVTPESYRGQTPPLDLARYFDGPLTG
                     WGYFADRSGEVTRRFTVRMTGVWQGDQGRLTEDFSWSDGTRSQRIWHITRQGPNHYRG
                     RADDVKGEAAGVAEGNALQWRYTLLLPVDDKVYEVQFDDWMYLIDDEVLLNRSQMRKF
                     GIRLGEVFIAFRRGA"
     gene            7372..8133
                     /locus_tag="PKI23_06110"
     CDS             7372..8133
                     /locus_tag="PKI23_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNN36546.1"
                     /translation="MSLNRPCVDWAGQRIWVIGASSGIGAALSRRLVELGARVAVSSR
                     NGAALAALGACHVEVVDITDRAALGHAFDAVRAALAEIDLVVVSAGTHQPMHAWELDA
                     EAAEALVQVNLIGVINVVALLAPYFVERGCGGIALIGSVAGYGGLPTALVYGATKAAL
                     INFAQTLHLDLAPRGVAVYLINPGFVSTPLTARNPFQMPALISAEAAARAIVAGITAG
                     RFEIHFPRRFTWVMKVLNWLPYRLYFALIRRVTGL"
     gene            8130..8579
                     /locus_tag="PKI23_06115"
     CDS             8130..8579
                     /locus_tag="PKI23_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNN36547.1"
                     /translation="MNSDALVDYFERLSPATLDQLDRFYAQAAWFKDPFHEVEGIEAI
                     GAILRHTFDRLPGARFRVTRQFSASDGDLMLLWELDFVMPITSAPTTIVGATHFAFDA
                     AGKVIRHRDYWDAAEELYGRLPLLKWLMRGLARRAAAPVPSARSTAE"
     gene            complement(8641..9183)
                     /locus_tag="PKI23_06120"
     CDS             complement(8641..9183)
                     /locus_tag="PKI23_06120"
                     /inference="COORDINATES: protein motif:HMM:NF025196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HNN36548.1"
                     /translation="MSPIHHGRHTARIEGDFVIFLIGMRVNRLRAIHKWLPVAQAMGP
                     MVKQLLQRPDLGLLHAQPCLYWRGVMLVQYWRSFEQLERFARDPAMTHLDAWKRFNQA
                     VGADGSVGIWHETYLVPAGHHECIYGNMPRTGLAQAGEHLPATGARETAKRRLGLKGE
                     PAVPSYPTPPTANEPGAAHR"
     gene            complement(9409..9912)
                     /locus_tag="PKI23_06125"
     CDS             complement(9409..9912)
                     /locus_tag="PKI23_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HNN36549.1"
                     /translation="MTAPTDDEPTPEPSSPACLMHEADDSYMGYVGRDELVAFLNELL
                     EAERAGARITLESAQLAGDTPVGALLRDIQQDEAHWCAMLLGHLRSLNAAPSPKVGAF
                     YDKAAAIAELDARVTFINRGQRWVVRKLCEMMPRLRDAGLRSDLEQMLRSHEANIALV
                     DAATGAV"
     gene            complement(9957..10550)
                     /gene="pgsA"
                     /locus_tag="PKI23_06130"
     CDS             complement(9957..10550)
                     /gene="pgsA"
                     /locus_tag="PKI23_06130"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNN36550.1"
                     /translation="MSDPVASHHLQWNVPNLLTAFRVVLIPVFVLVFYLPVEWRFLAA
                     GVIFIVASITDWLDGHLARQLDQATLFGAFLDPVADKLMVGIAMVLLVTRYPTAAFAI
                     PAAIIVGREIAVSALREWMAELGKRASVAVSYVGKVKTVLQMVAISILLLEPSAGSAW
                     LRIPGFIAIYLAALLTLWSMLVYLLAAWPLLTGQSEG"
     gene            10819..11637
                     /locus_tag="PKI23_06135"
     CDS             10819..11637
                     /locus_tag="PKI23_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNN36551.1"
                     /translation="MAYLAPAEFVTKMVDAGESKLLMSTRDTLIRAYMAGAILALAAA
                     FAVTVTVNTGNALVGALLFPVGFCMLYLLGFDLLTGVFTLAPLAVMDKRPGATWGGVM
                     RNWTLVFLGNFAGALTVALFMAIIVTFGFTEAPNAVGQKLGVIGESRTVGYAAHGAAG
                     MLTLFIRAVMCNWMVSTGVVAAIMSTSVSGKVIAMWMPILVFFYMGFEHSIVNMFLFP
                     SGLMLGGNFTLMDYFIWNEIPTVVGNLVGGLTFVGATLYSTHYKTAPKRTVAGR"
     gene            11677..13413
                     /locus_tag="PKI23_06140"
     CDS             11677..13413
                     /locus_tag="PKI23_06140"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HNN36552.1"
                     /translation="MSSQLRISAGQYSSAGRKPVNQDFHGLCIPREPLLSAKGIAVAL
                     ADGISSSEAGQIASEAAVKSFLADYYCTTEAWSVKRSAQRVLAATNAWLHAHTQRGQG
                     RFDKERGHVCTFSALVLRSTTAHLFHVGDTRIHRLHGQALEQLTTDHRVVVAPDESYL
                     SRALGIWQQIDLDYQALPLEVGDTFVLTSDGVHEQMDGAFVAATVARLHDDLDLAART
                     IVEEALRRGSGDNLTVQIVRVDALPDPEAAELPQLAAGLRPPPLPEPGALLDGYRIVR
                     ELHASHRSHLYLVQDEESGERLVMKVPSIDMREDAAYLERFLLEEWVARRIHSPHVLR
                     AAAQRRQRSALYVTLEYLEGQTLAQWMADHPTPDLETVRGMVEQIARGLQAFHRMEMV
                     HQDLRPENVMIDREGTLKIIDFGATRVASVMELLPEQRQHEMLGTLQYSAPEYLLAEG
                     ASNRADLFSLGVIAYQMLSGRLPYGIQVARLRRHAELRKLRYVPLYELDRRIPLWVDG
                     ALRKAVHPDPRQRYEEVSEFVWDLRHPNPAFLGRHRPPLIERNPLAFWKGVSLLLLVL
                     LLVLLATHPALR"
     gene            complement(13460..15304)
                     /gene="uvrC"
                     /locus_tag="PKI23_06145"
     CDS             complement(13460..15304)
                     /gene="uvrC"
                     /locus_tag="PKI23_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNN36553.1"
                     /translation="MNDLQPVVVSALIDAKLAQLPARPGVYRMFDAESRLLYVGKAKN
                     LRNRVRSYFRGRPLDGKTMAMVGRIADLDITLTPSETEALLLEQNLIKAHHPPYNIEL
                     RDGKSYPFIRLSGDTAFPRLSFYRGARKTDSRYFGPYPSAAAVRESLGLLQKMFRIRQ
                     CEESIFRNRSRPCLQYQIGRCSAPCVGLIDSEAYQQDMADALLFLQGRSSDLIDTLAN
                     RMEQAATALDYERAARLRDQIAQLKRMQAQQVVVGDGGDADVLAAHLQPGGACVTLLS
                     IRNGQLLGSQSYFPRAALVSTPAELLSAFIPQFYLDDNAGRAIPPQLLLSDAPDDQAC
                     LTEVLSSAAGHAVTLRSGTRGQRARWIDLALENARQQLTAHLANRLKMAQRFEALRES
                     LGLTQTPQRIECFDISHTSGEQTVASCVLFTSQGALKSDYRRFNISDITPGDDYAAMQ
                     QALTRRYVRLKRGEEPLPDLLLIDGGKGQLRQAREVLAALGITGVMLLGIAKGTSRKP
                     GLETLLFDDGSERTLPAHDPALHLLQQIRDEAHRFAITGHRQRRKRDQNSSPLELIEG
                     IGPKRRRELLRHFGGIQGLERAGIAELTQVPGINRPLAERIFNALHRL"
     gene            complement(15348..15986)
                     /gene="uvrY"
                     /locus_tag="PKI23_06150"
     CDS             complement(15348..15986)
                     /gene="uvrY"
                     /locus_tag="PKI23_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HNN36554.1"
                     /translation="MIKLLIVDDHELVRTALTCMLDSISGVQVVGQAANGEEAVQKSS
                     LLQPDVVLMDVRMPGIGGVEATRRILRHQPETRVIALTAYDDDPFPNRLLQAGAMGYI
                     TKDSNLDEMLNAIRKVHSGQRYISVKIAQELALKRFSGDADTSPFDLLSEREMQIALM
                     IIHCQKVAEISAKLHLSPKTVNSHRYNIFEKLGIDSDVALTHLALRWGLIDA"
     gene            complement(16045..16512)
                     /locus_tag="PKI23_06155"
     CDS             complement(16045..16512)
                     /locus_tag="PKI23_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNN36555.1"
                     /translation="MTAFKNVARASALSLLASVPVGLIYLIAVARISEPLPEAMGIAA
                     FILCGVVGVLLGARIASQPAPQRSGTASPHRSRSRPEAAPMPIGEREQGAVKWFNASK
                     GFGFISRDSGGDVFVHFRSIRGTGHRALREAQRVEFNIADGDKGLQAIEVDVL"
     gene            complement(16668..>18262)
                     /locus_tag="PKI23_06160"
     CDS             complement(16668..>18262)
                     /locus_tag="PKI23_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNN36556.1"
                     /translation="DWCISRQIWWGHRIPAWYDESGAVYVGHSEAEVRARHGIAPEQV
                     LRQDEDVLDTWFSSALWPFSTLGWPEQTPELGTFYPTSVLVTGFDIIFFWVARMIMMG
                     LKFTGEVPFREVYIHGLVRDAEGQKMSKSKGNVLDPIDLIDGITLPELIAKRTSGLMQ
                     PQMAQKIEQATRRQFPDGIPAFGTDALRYTFCSLASTGRDIKFDLGRIEGYRNFCNKL
                     WNAARFVLMNCEGQDTGLNDAPVTLGLAERWIISRLQRTEATVREQLDGYRFDLAASA
                     AYEFVWGEYCDWYLELAKTTLQDAAADAAVLRGTRRTLVRVLETALRLLHPFMPFITE
                     ALWQSVAPLAGRSGATVMTAPYPTPDASRIDAVAEADMEWLQAVVMAVRNIRGEMNIP
                     PGKRLPILLNRGSTQDRQRLESARAAIEALVRAESIFWLPPALTAPPSAIQLVGELEI
                     LVPMADLIDVAAERARLERELEKIDHELGRIATKLDNADFVARAPEAVVTRERDRRTE
                     LLAAREKLQAQHAQVSALGAAG"
BASE COUNT         2888 a         5611 c         6093 g         3570 t
ORIGIN      
        1 cggcaacggc atcggtcagt tgcaggccga ggatgtgggc ttcatcaaca ccgacatcag
       61 cctcaacctg caccgtctgc cgcaagggga gtggatctgc atcgatgccc gcacattggc
      121 gcagccttgc ggcatcggca tggtcgagac cgtgctgtac gaccacttgg ggccaatcgg
      181 ccgggtgacc caggccaatc tggccaatcc gctctaccgt ggttgagtcc gtgggcaacc
      241 cgcaggcacc gggggcgacg atgtatgtcg tgctgttccg cgcaaaaatc cgggcacccg
      301 atgatgacta cctgcagacc gcggcgcgaa tgcgcgagct ggccctgcag cagtttggct
      361 gtctcgagtt tcagtcgatg acgcaagggg aggaggaggt cgccatctcc tggtggccca
      421 gcgaggcggc gatcctggcg tggaagcagc accccgagca tctgctggcg cagcgcgccg
      481 gccgtgagcg ctggtattgc gcctactcgg tgcaggtggc ggccgtggtg cgcgactatc
      541 aggtggtctg ctgagggctg gatcatggct gcatggccat tggccggtgc agccgaagcc
      601 cctcatgcag ttgctcgatc cgaactcact gtccagagcg tgaatgagcg gccatgctgt
      661 cctggcgcgc tcgccagcgg cgcagagcgg catggaggtc gtcgaactcc ggggtcaaag
      721 cgatgccctc gatcggtcgg cggatcgcct tgatgccatg gccgccggcc cagagttcga
      781 cctcggtggg cagctgctgg cgcaggtcac gcagcccctg ctgtgccagt gccggcaggt
      841 aggccgaact gaacgagagg cagaccacat cgatccgttc gagccgtgcg gcgcgggcga
      901 tctcgctgac tggcgtctgc gctccgagct ggatcgggca ggcctcttcc agcgccagca
      961 gggcttccac catcaacagt cccagggtgt gctcctcgcc ggcgagggtg gtgagcagca
     1021 ctctcgggcg gccttgtggc tgcgacagat tggccagcgc gctgtgaaac agcttttcaa
     1081 gattctgcga gaacagatgc tcctcgaaca cgccgatctc ccggtttgac caggcggcac
     1141 cgacgtgggt gatcaaagga gcagcaatgt cctggacaaa acgggcgagt ccatggcggt
     1201 tgacttcgcg ttgcaggtca cgttgcaagg catcgctgtc gtgcaggcgc acccgttgca
     1261 gccactcatc gagcggttgc tgcgtgctct gcatggcgga aggcgctgcg gtgccgagca
     1321 gtgccgacaa cgcttcttca tcgagtttca gcagttttcc cggccgatgg ccctggtcga
     1381 gcagtcgctt gagctgcgtc agccgccgca cctgctgcag cgaatagctg cgttcgccgt
     1441 tcgcatcccg ttcgggtgtc ggaaagccgt agcggcgctc ccacacccgc agggtgtcct
     1501 tggacaggcc cgtttcgcgc tccactgaag agatgggcag gccgggcact gaatcatctg
     1561 gctgcatttt ttgatttgtc catgtcaaag cgttgacaga ttggcatttg acggctagtt
     1621 tagcgctaaa gatcagtttt gaccaagaca aattgacgtg tttgaccttg tgtgaaggtg
     1681 acacgactgg caatcgcagc gctggaggca gcaaagatga tgcaggtggc agtgattggt
     1741 tccggaatct ccggactggc gacagcctgg ctgttgagtc gtcggcaccg ggtcacgctg
     1801 ttcgaagcgg agcagcggct gggcggccac acccataccg tcgacctgac gctcgatggc
     1861 cagacctttg cggtggacac cggctttctg gtgttcaacc accgcaccta tccgcagttg
     1921 acgcagttgt tccgccagtt gggggtcgag acggtcgcca gcgagatgtc attcagtttt
     1981 cggatggatg agccggaact ggagtgggcc ggcagcagcc tggataccct gttcgggcag
     2041 cgtgccaacc tggtgcgccc gcctttctgg cagatgctcg gcgagatgct gcgtttcaat
     2101 cgcagcgcgg cccgtgacct gccgatgcat ggtgacctga cgctcggtga ctatctccgg
     2161 ctgcgtggct atggcagacc cttcatcgac tggtatctgc tgccgatggc ggcggccatc
     2221 tggtcggcgc cgacggcgcg catcctggag ttttcgctct catccttcgt ggccttctgc
     2281 aacaaccatg gcctgttgca gatcttcgat cggccgcgct ggtacacggt caagggcgga
     2341 gggcgcgaat atgtgcagcg gctggcggcg ggaatcgatc agattcgcct tggccagccg
     2401 gtcgattcac tgcgtcgccg tggcggactc atcgagttgg gcagtggtga ctttcagggt
     2461 cgatacgacc aggtggtgct ggcctgccac agcgatcaat cggcgctgct gctggagcgg
     2521 gattttccgc agcgggcgca gtggctgcga cacattccct atcagcacaa tcaggtggtg
     2581 ctgcacagtg acccgcgggt gctgccacgc cgccgcaagc tctggtcggc ctggaactat
     2641 caggcggttt catcagcagc tggtgcggtg cagtctggca ctggccgggc ggtggcagtg
     2701 cactacctga tcaatcggtt gcagccgctg ccgaccacga caccggtgat cgtctcgctc
     2761 aacccggtgg agccgatcga ccccggcctg gtgcatcgcc ggttcgacta cagccatccg
     2821 ctcttttcgt tcgacagcgc aaaggtgcag cagcagatca cggcgagcaa tggcgagcag
     2881 ggcatctggc tggcgggtgc ctggctcggt catgggttcc atgaggatgg tctggcttcg
     2941 gcgctgcggg tggcacgggc actcgatgtc gtctcgccgt gggagggtct ggccgatgct
     3001 gcctgagatg gcacagatcg gttttggtgg tctgcgccat ctgcggctga agccagtggt
     3061 gaaccgcttc agctatccgc tctttttcgt gcggttgccg atgcatgagc tgaccggggt
     3121 ggcagtgccc ggcttctcga tcgaccgctt caacctgctc agtttccatc atcgcgatca
     3181 tggcgagggt ggcgatgcac tgcgctgggt gcgcgacctg cttcaggcgc agggtctggt
     3241 ggctgatggc gccatctggt tgaccacctt tccccgtgtg ctgggctatg tcttcaagcc
     3301 ggtcagcttc tggcattgcc accgtgccga cggccaactg gtggcagtgc tggccgaggt
     3361 caacaacacc ttcggtgagc ggcatgtcta tctgctgcgc gctggagagc cagccggggc
     3421 attcgaagcg caaaaggtgt ttcacgtctc gccgttcttt gggcggcagg gtcgctatcg
     3481 cttcggtttt catgtcgagg ccggtcgatt ctgcgcgcgg atcgactatg acgatggcga
     3541 tggcctggcc ctcgcgaccc gctggtgggg caagctggaa cccctgactg cccgcagtgg
     3601 ccgtcgcgcc ttctggcgct atccgctgat gaccttggcg gtgatcgtgc gcattcatct
     3661 gcaggcagtg cgactgttgc gcaagcgggt gcgactcttt ccgaaacctg ttccctctcc
     3721 catcgaggtc accgaatgag cagcgtcgat ctgactgccg gtctgcaaca caacaagagc
     3781 ccatggtatg ccttgccggt cgtcaggcgg ctgcatggca tcgaaggcgg ggtcattcgc
     3841 ctggaactgc ccaacgggcg cacggtgcgt ctcggccagg ggggagtgga agtctcactg
     3901 cggctgcggc gctggagcct gctgcggggc attctcacca gtggcgacat cggctttgcc
     3961 cagggctata tcgatggcgg gtgggacacc gccgatctga ccgcgctgct caccctgttg
     4021 gcgcgcaacc ggacggcgct cggccatctg atccatggtc aatggctggg acggttgcag
     4081 caccgcctgc gccatctgcg caacgccaac agcttgaagg ggtcgcggcg caacattgca
     4141 ttccactacg accttggcaa tgcgttctat gcgctctggc tcgatcggac gatgacctac
     4201 tccagtgccc gcttcgacgg tgactggagc ctcgatctgg gtgaagccca gcagcgcaag
     4261 tatcagcggg tgctggagct gctcgatctg cccagcggac agtcgctgct ggagatcggt
     4321 tgcggctggg gtggctttgc cgaacaggcg gcaggcagtg ccggtcatcg ggtgcgcggc
     4381 ctcacgctgt cgcgccagca actggagtat gccagtgcgc ggatcgatgc agccggtctg
     4441 gccggtcagt gcggttttgc gttgcaggat taccgtgatg agcagggcca gtacgatggc
     4501 atcgtctcga tcgagatgtt cgaggcggtc ggcgaggcct attggaatga ctacttcgcc
     4561 gccgtcaggc gggccctccg gccgggagga agggcggtgg tgcagagcat tctgatcgac
     4621 gatgcgctgt tcgagcgcta tcgcaacggt accgatttca ttcagcaaca gatctttccg
     4681 ggtggcatgt tgccttcggc cgaacgcttc cgggcggtgg cggccgaaaa tggtctggtc
     4741 gtcacccatc aggagcattt cggcctcgac tacgccgaga cgctgaaccg ctgggaaaga
     4801 aacttcatcg cccgccagga cgaggtgcgt gcactcggtt tcgatgcgcg cttcatgcgc
     4861 ctttggcact tctatctggc ctactgccag gctggcttca acagtggttc gaccgatgtc
     4921 gcgcagttcg tgctccagcc ggcgctctag cgccgcactg ctgctgtggc tgacggtggt
     4981 ggcgctgccc gtcgtcgcga tggcgccatt gccggaattg cctttcgtgc cacggcaggt
     5041 tggtgccggc aacctgcgcc atttcggtat ccagctgtat gaggcccggc tgtggaatgg
     5101 cgatggcctc tggaaaccgg acgcacccta tgcgctggaa ctgatctatg cccggtcgtt
     5161 caccgccgag gccattgccg agcgcagcat tgtcgagatc cgtcgtcagg ggatcgacga
     5221 tgccctgttg ctggcgaact ggcaaacccg gttgcgggcg ctctttccgg atgttgccgc
     5281 cggcgaccgg ctgctcggtc tgcgtcaacc cggggcgggt gccaccttct atctgggtga
     5341 gcgtcagatc ggccgcatcg atgatgaaca actggctgct gcctttttcg ccatctgggt
     5401 cggcgaacag agcagcgcgc ccggattgcg cagacgactg ctcggcctgc catgaccacg
     5461 ccgctgctgc catcccacca gctcatcgcc tacggcgccc tgggtctgcc gctggccttt
     5521 gccgccttgc cgctctatgt ccatctggtg cctttctatg ccgagagcat ccctcttgcg
     5581 ctgctcggca tcctgctgct ggcgctgcgc ttcttcgatg cggtgatcga tccgctgctc
     5641 ggcgtgctgc tcgatcggtt tccccatcca cgcgggttga tagcgctctc tctgccgcta
     5701 ttgggtgtgg gctggatggg gctctttgtc ccgcagcgac cctggccacc gataccctgg
     5761 ttgctgacga tgctgacgct gaccacggtc ggttacagtc tggccaccgt ggtgcacaac
     5821 gcctgggccg cgcggctgtg tgcaactgcc gcagaacggg cccgactgtt cgcatcccgc
     5881 gaggcattcg gtctggccgg tgtggtgctg gccgccagcc tgccgaccct gctggcaccg
     5941 accctctcca cggggctgac gcggacaggc ccctggtttc tgctgttgct gatcgctctg
     6001 ggtggcgtga ccctgctcgg gacccagcgg accgtcctgc gcgtggagcc gttcgcccgc
     6061 acccagtggc tgcgcccctt tgcccaacca ctcttcaggc ggttcgcacc cggttacctg
     6121 ctgagcgccg tggccagtgc gctgccggcc tcgctggtgt tcttcttcat cgatgacgtg
     6181 ctgcggatgg gtgcctgggc agggctgttt cttgccctct atttcattgc cggcgcggcc
     6241 ggactgccat tgtggatgag gctggtacat cggatcggcc tcttcgatgc ctggctgctg
     6301 tcgatggtgc tggccatttt cacctttgcc tcggccttca cgctgggtgc cggtgatggg
     6361 ctgggctatg ccctcatctg cgtcgcctcc ggcgtggcac tgggggccga tctggcgctg
     6421 ccctctgcac tggtggccga gatggtcgat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn ngcactgggg gccgatctgg cgctgccctc tgcactggtg gccgagatgg
     6601 tcgatgtcga tcagggcagt caacccggcc tctactacgg tctgatgagc tttctggcga
     6661 agctggcact ggccctggcg gccggcctgg cgttgccgct gctcgatctg tggggctacc
     6721 agccgggcgg tgagcagttg tggccgctct cgtttgccta tgcgctgctg ccctgtctgc
     6781 tgaagctggc cgccctggcc tggttccggc cgctgcgtcg ctttttcgcc actggaggtg
     6841 tcacatgctg aaaagatcgc tgattgcact ctgcaccttg ctggggttga gcggttgcgc
     6901 cggggtcacg cccgagagct accgcggtca gacaccacca ctcgatctgg cccgatattt
     6961 cgatggacca ctcaccggct ggggctactt tgccgaccgc tctggcgagg tgacccgccg
     7021 cttcacggtg cgcatgaccg gcgtctggca gggtgatcag ggccggctta ccgaagattt
     7081 ttcctggtcg gatggcaccc gctcacagcg catctggcac atcacccggc agggcccgaa
     7141 ccactacagg ggccgggcgg atgatgtcaa gggtgaagcc gccggcgttg ccgagggcaa
     7201 cgcgttgcag tggcgctaca ccctgctgct gccggtcgat gacaaggtgt atgaggtgca
     7261 gttcgatgac tggatgtacc tgatcgacga cgaggtgctg ctgaaccggt cgcagatgcg
     7321 caagttcggc atccgcctcg gtgaagtgtt catcgccttc aggaggggag catgagtctg
     7381 aatcgcccct gtgtcgattg ggccggccag cggatctggg tgatcggcgc cagctccggc
     7441 attggtgccg cgttgagtcg ccgactggtg gagctcgggg cacgggtggc ggtcagcagc
     7501 cgcaatggcg cggcattggc cgcgctgggg gcctgccatg tcgaggtggt cgacatcacc
     7561 gaccgcgccg cgctcggtca cgccttcgac gcggttcgtg cggcactcgc agagatcgat
     7621 ctggtggtgg tgagtgccgg cacccatcag ccgatgcatg cctgggagct ggatgccgag
     7681 gccgccgagg cgctggtgca ggtcaatctg atcggggtca tcaatgtcgt ggccctgcta
     7741 gccccgtact tcgtcgagcg cggctgtggt ggcattgcgc tgatcggcag tgtcgccggt
     7801 tatggcggcc tgcccacggc attggtctat ggcgccacca aggcggcgct gatcaacttc
     7861 gcccagacgc tccatctcga tctcgcgccg cgtggtgtgg cggtctatct gatcaacccc
     7921 ggattcgtca gcaccccgct cacggccagg aaccctttcc agatgccggc gttgatcagt
     7981 gccgaggcgg cggcgcgggc catcgtggct ggaatcacgg ctggcaggtt cgagattcac
     8041 tttccgcgac gcttcacctg ggtcatgaaa gtgttgaact ggctgcctta ccggctctac
     8101 ttcgcgctga ttcgtcgggt caccgggctg tgaacagcga tgccctggtc gactatttcg
     8161 aacggctctc cccagcgacg ctggatcagc tcgatcgctt ctatgcgcaa gcggcctggt
     8221 tcaaggatcc tttccacgag gtcgagggca tcgaggccat tggtgccatc ctgcgacaca
     8281 ctttcgaccg gctgccgggc gcccggtttc gggtgacacg gcagttcagc gccagcgatg
     8341 gcgatctcat gctgctgtgg gagctggatt tcgtcatgcc gatcaccagt gcgcccacca
     8401 ccatcgttgg tgccacccat tttgcgttcg atgccgccgg caaggtgatt cgccaccgcg
     8461 actactggga tgccgccgag gagctctatg gccgcctgcc gctgctgaag tggctgatgc
     8521 gcggtctggc ccgccgggca gccgcgccgg ttccgtcagc gcgaagcaca gcagagtgag
     8581 cgaacggcct gatccgtatc cgatcactta accggccttg cggtcgggca gggtggaggt
     8641 tcagcgatgg gctgcgccgg gttcgttcgc ggtgggtggt gtcgggtagc ttggcaccgc
     8701 cggctcgccc ttcaggccca gacgccgctt ggcggtctcc ctggcaccgg ttgcaggaag
     8761 atgttcgccg gcctgcgcca gtcccgtgcg cggcatgttg ccatagatgc actcatggtg
     8821 acctgcgggc acgaggtagg tctcatgcca gatgccgacg ctgccatccg caccgacggc
     8881 ctggttgaag cgcttccagg cgtcgaggtg ggtcatggcg ggatcgcgtg caaagcgttc
     8941 gagctgttcg aacgagcgcc agtactgaac gagcatgacg ccccgccaat acaggcaggg
     9001 ctgcgcatgc agcagaccca gatcgggtct ttggagcagt tgcttcacca ttggccccat
     9061 ggcctgcgcc accggcagcc atttgtggat ggcgcgcaat ctgttcacgc gcatgccgat
     9121 cagaaagatg acgaaatcgc cttcgatccg ggcggtgtgg cggccatgat gaatcgggct
     9181 catggggctg ctcctggggt gagactctcc gtcgagctgg cctgacacgc cgcgcaggca
     9241 tggcagaagc gtgagaaggc attcctgcgc ctgttgcact tgacgcagtg tggacagaaa
     9301 tcgatctcgc tgtttttcag gtcgactgtg cgctcgcaac caggacagac accctcggcc
     9361 attctggcca gcgcggtgtc gtagctcagt gcctgtcggc gcaccggatc agaccgcgcc
     9421 ggtcgctgca tcaaccaaag cgatgttggc ctcgtgtgag cgcagcatct gctccagatc
     9481 gcttctgagc ccggcatcgc gcagtcgcgg catcatttcg cacagtttgc gcaccaccca
     9541 gcgctggccg cggttgatga aggtgacgcg tgcgtccagc tcggcaatgg ccgccgcctt
     9601 gtcgtaaaag gcgccgacct tcggcgacgg cgccgcattc agtgaacgca gatggccgag
     9661 cagcatggcg caccagtgtg cctcgtcctg ctggatgtcg cgcagcagcg cgccaactgg
     9721 cgtgtcgccg gccagttgtg cgctctccag ggtgatgcgg gcgccggcac gttcggcttc
     9781 gagcagctcg ttgaggaagg ccaccagttc atccctgccg acatagccca tgtagctgtc
     9841 gtcggcttcg tgcatcagac aggcgggcga gctcggttcc ggggtcggtt cgtcatcggt
     9901 cggtgcagtc atggtgcaag gctccctgga tgagtggtcc gattgaaggc cgcaggtcac
     9961 ccttccgatt ggccggtcag cagtggccag gcggccagca gatagaccag catcgaccag
    10021 agggtcagca gcgcggccag atagatggcg atgaagccgg gaatgcgcaa ccaggcgctg
    10081 ccggcgctcg gttcgagcag caggatcgag atggccacca tctgcagcac ggtcttcacc
    10141 ttgccgacat aggagacggc gacgctggca cgcttgccca gctcggccat ccattcacgc
    10201 agtgccgaga cggcgatctc ccggcccacg atgatggcgg ccggaatggc gaaggcagcg
    10261 gtgggatagc gggtcaccag cagcaccatc gcgatgccga ccatcagctt gtcggcgacc
    10321 gggtcgagaa aggcgccgaa cagggtggcc tggtcgagct ggcgcgccag atggccatcg
    10381 agccagtcgg tgatgctggc gacgatgaag atgaccccgg ccgcgaggaa gcgccactcg
    10441 accggcaggt agaagaccag cacgaagacc ggaatcagca ccacccgaaa ggcggtcagc
    10501 aggttgggga cattccattg caggtggtgg ctggccacgg gatcggacat ggaaagagtt
    10561 ccgtcaggcc cgccgcaggg cgttgcggac aggcctgcgg gcgggggttg atggttgatg
    10621 tctgggccgg tctggcgctg gcgagtcagt ctagcacagc gtcatgccgg cgctgctgca
    10681 tcgtcgtgag ccatggagaa cgcagagggg ccgtttattt ggcaggtttg ttgctatgcg
    10741 atgcgacatc catcgaggtc ggatgccacc tcgatggccg gcactctgct gtctcattca
    10801 ttcaccaagg agcccaccat ggcctatctc gcacccgctg aattcgtcac caagatggtc
    10861 gacgccgggg aatccaaact gctgatgtcg acccgcgata ccctgattcg tgcctacatg
    10921 gccggcgcca tcctggcgct ggccgccgct tttgcggtga cggtcacggt caacaccggc
    10981 aatgcactgg tcggcgcgct gctgttcccg gtcggcttct gcatgctcta cctgctcgga
    11041 ttcgatctgc tcactggcgt cttcacgctg gcgccgctgg cggtgatgga caagcggcca
    11101 ggcgccacct ggggcggcgt gatgcgcaac tggaccctgg tgttccttgg caatttcgcc
    11161 ggtgcgctga cggtggcgct gttcatggcg atcatcgtca ccttcggctt caccgaggcg
    11221 cccaacgcgg tgggtcagaa actgggcgtg attggtgaga gccgcacggt cggttacgct
    11281 gcccacggtg ccgccggcat gctgaccctg ttcatccgcg cggtgatgtg caactggatg
    11341 gtctccaccg gtgtggtggc ggccatcatg tcgacctcgg tgtcgggaaa ggtgatcgcg
    11401 atgtggatgc cgatcctggt cttcttttac atgggcttcg agcactcgat cgtgaacatg
    11461 ttcctgttcc cgtcgggctt gatgctgggc ggcaacttca cgctgatgga ctacttcatc
    11521 tggaacgaga tcccgaccgt ggtcggcaat ctggtcggcg gattgacctt tgttggtgca
    11581 acgctctatt cgacgcatta caagaccgct ccaaaacgca ccgtcgcggg gcgttgatcg
    11641 agcaggcttc ggcactgaac ctgttgcgtg gcgcacatgt ccagtcaact gaggatcagt
    11701 gccggccagt acagcagcgc cgggcgcaag ccagtcaatc aggattttca cggcctgtgc
    11761 attccgcgcg agccgctgct cagtgccaag ggcatcgccg tcgcgctggc cgatggcatc
    11821 agcagcagtg aggccggcca gatcgccagc gaggcggcgg tcaagagctt tctcgccgac
    11881 tactactgca cgactgaggc gtggtcggtc aagcgttcgg cccagcgggt gctggcggcc
    11941 accaatgcct ggctccatgc gcacacgcag cgcggccagg ggcgcttcga caaggagcgt
    12001 ggccatgtct gtactttcag tgcgctggtt ctgcgctcga ccacggcgca cctgtttcac
    12061 gtcggcgaca cgcgcatcca ccgcctgcac ggccaggcac tggagcagtt gacgactgat
    12121 catcgggtcg tggtcgcgcc ggacgagagc tatctgagcc gtgccctcgg catctggcag
    12181 cagatcgatc tcgactacca ggcactgccg ctcgaagtcg gcgacacctt tgtgctgacc
    12241 agcgatggtg ttcatgagca gatggatggc gctttcgtgg ccgccaccgt ggcgcggctg
    12301 catgatgatc tcgatctggc tgcccgcacc attgtcgagg aggcgctgcg gcgtggcagc
    12361 ggtgacaacc tgaccgtgca gatcgtgcgg gtcgatgcgc tgccggaccc ggaagccgcc
    12421 gaactgccgc agttggccgc cggcctgcgg ccgccgccat tgccggaacc gggcgcgctg
    12481 ctggatggct accgcatcgt tcgcgaactg catgccagcc accgcagcca cctctacctg
    12541 gtgcaggacg aggagagcgg tgagcggctg gtgatgaaag tcccctccat cgacatgcgc
    12601 gaggatgccg cctacctgga gcgctttctg ctcgaggagt gggtggcgcg gcgcatccac
    12661 agtccccacg tgttgcgcgc ggcggcgcag cggcgccagc gcagcgcgct ctatgtgacg
    12721 ctggagtacc ttgaagggca gacactggcg caatggatgg ccgatcatcc gactccggat
    12781 ctggagacgg tgcgtggcat ggtcgagcag atcgcccggg gattgcaggc ctttcatcgc
    12841 atggagatgg tgcatcagga tctgcggccg gaaaatgtca tgatcgaccg cgagggcacg
    12901 ctgaagatca tcgacttcgg cgccacccgc gtcgccagtg tcatggagct gctgcccgag
    12961 cagcgacagc atgagatgct cggcacactg caatacagcg cgccggagta cctgctcgcc
    13021 gaaggggcat cgaaccgcgc cgatctcttt tcactgggcg tgatcgccta ccagatgctc
    13081 agtggccgcc tgccttatgg cattcaggtc gcgcggctgc gccgccacgc cgagctgcgc
    13141 aagttgcgct atgtgccgct ctacgaactc gaccggcgca ttccgctgtg ggtcgacggt
    13201 gcgctgcgca aggccgtgca tccggatccg cggcagcgct acgaagaggt gtccgaattc
    13261 gtctgggatc tacgtcaccc gaaccccgca ttcctgggcc ggcatcggcc gccgctgatc
    13321 gaacgcaacc cgctcgcatt ctggaaaggg gtttcactgc tgctgctggt gctgctgctg
    13381 gtgctgcttg ccactcatcc ggcgctgcgc tgatcagggg tgatttccgc gcctgaaccg
    13441 aggcgcgcag cgcctcgcgt cagagccggt gcagggcgtt gaagatgcgt tcggccagcg
    13501 ggcggttgat gcccggcacc tgggtcaatt cggcgatgcc ggcccgctcc agcccctgga
    13561 tgccgccgaa gtggcgcagc agttcgcgcc ggcgcttcgg gccgatccct tcgatcagct
    13621 ccagcggtga gctgttctgg tcgcgtttgc ggcgctggcg atggccggtg atggcaaagc
    13681 ggtgcgcctc gtcacggatc tgctgcaaca ggtgcagtgc cggatcgtgt gccggcaacg
    13741 tccgctcact gccatcgtcg aacagcaggg tctccagacc cggcttgcgg ctcgtcccct
    13801 tggcgatgcc cagcagcatg acgccggtga tgccgagggc cgcgagcacc tcgcgggcct
    13861 gacgcaactg tcccttgccg ccatcgatca gcagcaggtc gggcagtggc tcctcacccc
    13921 gtttgagtcg gacatagcgg cgcgtcagtg cctgttgcat ggcggcgtag tcgtcgccgg
    13981 gggtgatgtc gctgatgttg aagcggcggt agtcgctctt gagcgcgccc tggctggtga
    14041 agagcacgca ggaggcgacg gtctgttcgc cggaggtgtg gctgatgtcg aagcattcga
    14101 tgcgctgtgg tgtctgagtc agtccaagcg attcgcgcag tgcctcgaac cgctgcgcca
    14161 tcttcagccg gttggccaga tgggcggtca gttgctggcg ggcattctcc agtgcgaggt
    14221 cgatccagcg tgcgcgctgc ccgcgcgtgc cgcttctgag cgtgacggca tggccggcgg
    14281 cgctgctcag cacttcggtc aggcaggcct ggtcgtccgg tgcgtcgctg agcagcagtt
    14341 gcggcggaat cgctctgccg gcgttgtcat cgaggtagaa ctgcggaatg aaggcgctga
    14401 gcagctcggc cggggtcgac accagcgccg cgcgtggaaa gtaactctgg ctgcccagca
    14461 gttggccatt gcgaatcgac agcagggtga cgcaggcgcc acccggctgc agatgggcgg
    14521 ccagcacatc ggcatcgcca ccatcaccga ccaccacctg ttgtgcctgc atccgtttca
    14581 gttgggcgat ctggtcgcgc agccgtgccg cgcgctcgta gtcgagcgcc gtggccgcct
    14641 gttccatgcg gttggccagt gtgtcgatca ggtcgctgct gcgcccttgc agaaacagca
    14701 gcgcatcggc catgtcctgt tgatacgctt cactgtcgat caggccgacg cagggcgcgc
    14761 tgcagcggcc gatctggtac tgcaggcagg ggcgggagcg gttgcgaaag atgctctcct
    14821 cgcactgccg gatccggaac atcttctgca gcaggccgag gctctcacgc accgcggcgg
    14881 cactggggta ggggccgaag tagcgcgaat cggttttgcg ggcgccgcga tagaagctga
    14941 ggcgcgggaa tgccgtgtcg ccggagagtc ggatgaaggg gtaggatttg ccatcgcgca
    15001 attcgatgtt gtagggcgga tggtgggcct tgatcaggtt ctgctcgagc agcagcgcct
    15061 cggtctcgct gggggtgagg gtgatgtcga ggtcggcgat gcgcccgacc atcgccatgg
    15121 ttttgccatc gagcggacga ccgcgaaagt agctgcgcac ccggttgcgc aggttcttcg
    15181 ccttgccgac atagagcagc ctgctctcgg catcgaacat gcggtagacg ccggggcggg
    15241 ccggcagttg tgccagcttg gcatcgatga gggcagagac caccaccggc tgcaaatcgt
    15301 tcatcggcga tcctggaggt aaaacggcgc caggcggttg ccgcggttca cgcgtcgatc
    15361 agtccccagc gcagtgccag atgggtcagc gcgacatcgc tgtcgatgcc cagtttctcg
    15421 aagatgttgt agcggtggct gttgaccgtc ttggggctca ggtgcagctt tgcggagatc
    15481 tccgccacct tctggcagtg gatgatcatc agcgcgatct gcatttcccg ttcggagagc
    15541 agatcaaacg gtgaggtgtc ggcatcgccg ctgaaccgtt tcagcgccag ctcctgagca
    15601 atcttgacgc tgatgtagcg ctggccgctg tgcaccttgc ggatggcgtt cagcatctcg
    15661 tcgaggttcg aatccttggt gatgtagccc atcgcgccag cctgcaacag ccggttgggg
    15721 aaggggtcgt catcataggc ggtcagtgcg atcacccggg tctcgggctg gtggcgcagg
    15781 atgcgtcggg tggcctcgac gccgccgata ccgggcatgc gcacatccat cagcaccaca
    15841 tcgggttgca gcaacgatga tttctgcacc gcttcttcgc cattggcggc ctgccccacc
    15901 acctgcacgc cggagatgct gtcgagcatg caggtcagcg cggtgcggac cagctcgtgg
    15961 tcatcgacaa tcagcaattt gatcaagtct gcacctcagg gagagcgagg ccggcatccc
    16021 tgtcggcacg ggcagggctg ctgattacag aacatcgacc tcgattgcct gcaacccctt
    16081 gtcgccgtcg gctatgttga attcgacccg ctgcgcttcg cgcagggcgc ggtgaccggt
    16141 gccgcgaatc gagcgaaagt gcacgaagac atcgccgccg ctgtcacggc tgatgaagcc
    16201 gaaccccttg ctggcattga accacttgac cgcgccctgt tcacgctcgc cgatcggcat
    16261 cggtgccgcc tcggggcggc tgcggcttcg gtgtggtgac gcagtgcccg aacgctgcgg
    16321 tgctggctga ctggcgatcc tggcgccgag cagcacgccg accacgccac agaggatgaa
    16381 ggccgcaatg cccatcgcct cgggcagcgg ttcgctgatc cgcgccaccg cgatcagata
    16441 gatcagtcca accgggacac tggccaacag ggagagggcc gaggcccggg ccacattctt
    16501 gaaagctgtc atgcgaatct cgattttcga tggtttggtg tgggtgaaaa ataaaaagtt
    16561 cagaatcaaa aggttgaaca ttctgactga agcgcgcagg gcgccacgca gaaggttctt
    16621 cccgtctcat tctaagaaaa aacggtggtg tcacgaaagg gcatcgttca acccgccgct
    16681 ccgagtgcgc tcacctgtgc atgctgcgcc tgcagtttct cgcgggcagc gagcagttct
    16741 gtccggcggt cccgctcccg ggtcaccacc gcttccggtg cacgggcgac gaaatcggcg
    16801 ttgtcgagtt tggtggcgat gcgtcccagt tcatggtcga tcttctccag ctcacgctcc
    16861 agtcgggccc gttcggcggc cacatcgatc agatcggcca tcggcaccag aatctccagt
    16921 tcgccgacca gttggatggc gctgggcggt gcggtgagcg cgggcggaag ccagaagatc
    16981 gactcggccc gcaccagggc ctcgatggcg gctctggcgc tctccagccg ttgccggtcc
    17041 tgtgtcgagc cccggttcag caggatcggc agccgtttgc cgggcgggat gttcatctcg
    17101 ccacggatgt tgcgcaccgc catcaccacc gcttgcagcc actccatgtc cgcctcggcg
    17161 accgcgtcga tgcggcttgc atccggtgtg ggataagggg ccgtcatgac ggtcgccccg
    17221 ctgcggccgg ccagcggggc gacgctctgc cacagcgctt cggtgatgaa gggcatgaag
    17281 gggtgcagca ggcgcagtgc ggtttccagc acccgtacca gggtgcggcg ggtgccgcgc
    17341 agcacggccg catcggccgc ggcatcctgc agcgtggtct tggccagctc cagataccag
    17401 tcgcagtact cgccccagac gaactcatag gcggcgctcg ccgccagatc gaagcggtag
    17461 ccgtcgagct gctcgcgcac cgtggcttcg gtgcgctgca gccgcgagat gatccagcgt
    17521 tcggccagtc ccagcgtgac cggtgcatca ttgagtccgg tgtcctgtcc ctcgcagttc
    17581 atcagcacga agcgggcggc attccacagc ttgttgcaga agttgcggta gccttcgatg
    17641 cgccccaagt cgaacttgat gtcgcggccg gtggaggcga gcgagcagaa ggtgtagcgc
    17701 agggcgtcgg tgccgaacgc tggaattcca tcgggaaact ggcgtcgcgt cgcctgttcg
    17761 atcttctggg ccatctgcgg ctgcatcagg ccgctggtgc gcttggcgat gagctccggc
    17821 agggtgatgc cgtcgatgag atcgatcggg tcgagcacgt tgcccttgga cttggacatc
    17881 ttctggcctt cggcgtcgcg caccaggccg tggatgtaga cctcgcggaa cggcacctcg
    17941 ccagtgaatt tgagccccat catgatcatg cgggcgaccc agaagaagat gatgtcgaag
    18001 ccggtcacca gcaccgaggt gggatagaag gtgccgagct ccggcgtctg ttcgggccag
    18061 cccagcgtcg agaagggcca cagcgccgag gagaaccagg tgtcgagcac gtcctcgtcc
    18121 tggcgcagca cctgctcggg cgcgatgccg tggcgggcac gcacctcggc ttcggagtga
    18181 ccgacgtaga ctgcgccgct ctcgtcatac caggccggga tgcggtggcc ccaccagatc
    18241 tgccggctga tgcaccagtc ct
//
LOCUS       DAORCQ010000042        18251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_1900_length_18251_cov_5.559793, whole genome shotgun sequence.
ACCESSION   DAORCQ010000042 DAORCQ010000000
VERSION     DAORCQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 18251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18251
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_18251_cov_5.559793"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PKI23_06165"
     CDS             <1..1415
                     /locus_tag="PKI23_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HNN36557.1"
                     /translation="PPNSDERYKFDTYTQHVLQGFPNTEKYETEIKRRFIEEPANTKL
                     LIVVSKLLTGFDAPSCTYIYLDNALRDHNLFQAICRTNRLDGADKDYGHIVDFKELFG
                     DVQEAIAVYSSDELDTDQGNGGDNNVHLKDWLVEGRRKLDAAHEALRYLCEPVPLPRE
                     VEQYLHYFCGDAANANALNETEPLRVAYYKAVALFARAWSELAQNLAEAGYTDAEAAA
                     LRQEVEFHAEIRAAIKKHSGEELDIKPYEADMRHLINTYIQADAAAGLGQLGDLSLVE
                     LIVATGIHDAIARKLNEKGRLSKNAIAEGIINNLRKTIIREQLTDPRFYEQMSLLLDD
                     LIRQSRADSAAYEEFLRKAEDLAKRVAAKQPDADLPAALHGKREAAVIYNNLPRILAA
                     ADAGLADQVAEASPEYADERLRLALEVDRVMREHAPAGWKGDQAKEAQVLNALFPLLN
                     RDRAATLALFELVKNQPGYA"
     gene            1412..2107
                     /locus_tag="PKI23_06170"
     CDS             1412..2107
                     /locus_tag="PKI23_06170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017001396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNN36558.1"
                     /translation="MTEMLHFGDLSIELTRKAVKHAHLSVHPPEGRVTLVAPIDTRTE
                     VARAYVVSKLRWIRDQRAGFAVQAREPPRRLVTRETHYLWGRRYLLEVEHRDGKPVIS
                     LDHRRMRMAVRPDSSLAVRARVLHDWHKSLLHDVVPDLIAKWEPRLGVRVRAYFLQRM
                     KTKWGSCNPRAGHIRLNTELVKKPKDLLEYVIVHEMLHLIEPTHSERFLGLMDRHYPV
                     WREARAELNELPL"
     gene            complement(2182..2257)
                     /locus_tag="PKI23_06175"
     tRNA            complement(2182..2257)
                     /locus_tag="PKI23_06175"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2222..2224),aa:Phe,seq:gaa)
     gene            complement(2312..2584)
                     /locus_tag="PKI23_06180"
     CDS             complement(2312..2584)
                     /locus_tag="PKI23_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNN36559.1"
                     /translation="MTRMVFCRKYKREMEGLDAPPYPGPRGEAIYQQVSKQAWQEWLA
                     HQTRLINERRLNMMESASRKFLQEEMERFLSGEALTAVEGYVQPER"
     gene            complement(2599..3675)
                     /gene="mutY"
                     /locus_tag="PKI23_06185"
     CDS             complement(2599..3675)
                     /gene="mutY"
                     /locus_tag="PKI23_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNN36560.1"
                     /translation="MKKTADTASVPPPTLLTSRILAWYHDHGRQRLPWRTQITPYRVW
                     IAEIMLQQTQVATATGYFERFLVAFPALEHLAAAPLDQVLHRWSGLGYYARARNLHRT
                     ARLLVEQHQGCFPTTVEQLAALPGIGRSTAGAILSIALGVRAPILDGNVKRLLSRLHG
                     VEGWSGERSVEQRLWQLAEAHTPATAFSDYTQAVMDLGALVCTRARPHCDACPVAQQC
                     VARQQGRTAELPTPRPRRSVPVRELTWLLLRNASQSLLLEQRPATGVWGGLWAFPESV
                     IDVDIDHEVQARWGFSVMAVTPLPHFEHLFTHLRLRVRPQLIDVALADDALLHQGVRW
                     CNIQQPCRVGLAAPVTRLLRQLQP"
     gene            3878..5704
                     /locus_tag="PKI23_06190"
     CDS             3878..5704
                     /locus_tag="PKI23_06190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNN36561.1"
                     /translation="MNSTAHHLLLKRLLVVLAGWLVLHALSLQAELLQLDPEVRDYPV
                     TGSLQVLRDPRHRFSINELLQGTAHGQWQQFTASSPPNFGLTRDAIWLRLPIEQRART
                     TQWLLEMSNPLLDQVDFHLVRNGRVLAHFITGDHYPFVQRPRAHRHLLFPLDLEAEGR
                     YEIWLRVESSSTMNLGLHLWQPDQFWKKDQPLLLGYGFFHGMLMLFSLYYLFIFAWTR
                     ERSHLYFALFSLSSALTVLTIHGFGFQYLWPNAIEWQEQAIFFLVPMACLTLGKFAGH
                     LLQLRGWRQLRPLLLSLAGWLSLLYMVASPLLPPQPRMVLLGALLLYSFGITFVISLL
                     ELFRGNRSIRYFILGWTLFLLCVLVFVLSRLGWLPPLPNAENAIEVGLAGLIIMLSFA
                     LTARLNEERRSKLAAQQEALHHEQQLVREQQHSLDIMRQASAELEQNVARRTHDLNVA
                     LKKLEHANAQLQLLSTVDSLTGLKNRRYFDQIYHSEWRRALREQTPLSLLLIDADHFK
                     RINDQLGHPAGDECLRQLAQRICSAVARPSDLVSRYGGEEFAVLLPSTALTGALQIAE
                     RIREQVKAEAVVWQEQPIWLTVSIGVASLKIDSSITPDLIVR"
     gene            complement(5779..6852)
                     /gene="hemE"
                     /locus_tag="PKI23_06195"
     CDS             complement(5779..6852)
                     /gene="hemE"
                     /locus_tag="PKI23_06195"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019363995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNN36562.1"
                     /translation="MTALINDRLLRALRREPVDVTPVWLMRQAGRYLPEYRATRSRAG
                     DFMSLCQSPQLALEVTMQPLERFALDAAILFSDILTVPDAMGLGLYFEEGEGPRFRQP
                     VTTPAAIEALPIPDAERDLGYVMDAVRLIRRELAGRVPLIGFSGSPWTLATYMVEGQS
                     SRDFRTIKRMLLDQPEALELLLDKLTLAVTDYLNGQIAAGVQAVQIFDTWGGTLTTGW
                     YERFSLRPMARIVAGLNPAPDGSRVPVILFTKQGGLWLERIAATGCDALGLDWTVDIG
                     EARARVGDRVALQGNLDPVVLYASPAAIRHEVGDILARYGAGNGHVFNLGHGIHPQIE
                     PDHVAALVDAVHELSLPYHQSQG"
     gene            complement(6916..8334)
                     /locus_tag="PKI23_06200"
     CDS             complement(6916..8334)
                     /locus_tag="PKI23_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019363994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNN36563.1"
                     /translation="MSDRLNNDFQFIQVGRSDPSRKPIRVRKRNFIEIYDLFEQKQAE
                     QQAHRCLDCGNPYCEWKCPVHNYIPNWLRLLTEGNIIEAVELCHETNTLPEVCGRVCP
                     QDRLCEGACTLNTKFGAVTIGSAEKYITDTAFAMGWRPDLSHVVPTGKRVAIVGAGPA
                     GLGCADVLTRNGVKAVVFDRHPEIGGLLTFGIPEFKLEKQVMALRREIFTDMGIEFRL
                     NTEVGRDITMEQLLAEYDAVFLGMGTYDTMKGGFPGEEIAGVIEALPYLIGNVSHHLQ
                     LPMEQYPHINLKGQRVVVLGGGDTAMDCNRSAIRQGAKSVTCAYRRDEANMPGSKREV
                     KNAREEGVHFLFNRQPVEIVGQWRVEGVKVVETRLGEPDEKGRRRPEPIPGSEEILPA
                     DVVLLAFGFRPSPAPWFAEHGIAVNSWGGVEVGQPGAFQFQTANPKIFAGGDMVRGSD
                     LVVTAIWEGRQAAEGILDALGV"
     gene            complement(8369..12823)
                     /gene="gltB"
                     /locus_tag="PKI23_06205"
     CDS             complement(8369..12823)
                     /gene="gltB"
                     /locus_tag="PKI23_06205"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNN36564.1"
                     /translation="MQTGLYHPDEFKDNCGFGLIAQIEGRTSHALLRSAIDALNCMTH
                     RGGIAADGKTGDGCGLLMQKPDRFLREVAGELFGRPLAELYSCGMVFLSQQESAALSA
                     RQALEQALIGEDLVVTGWRPVPTDPTVLGPIALGNMPRFEQIFVEAPAGLDERTFNVR
                     LFIARRKAERAVAPVDQEFYITSLSSKVLCYKGLMMPADLPRFYPDLHDARMETAICT
                     FHQRFSTNTLPKWPLAQPFRLLAHNGEINTIQGNRNWVSAREARMSTPWLPDIAAISP
                     LVNREGSDSSSMDNMLETLLAGGVDLYRAVRMMIPPAWENVETIDTDLRAFHEYHSML
                     MEAWDGPAGIVLTEGSTAVCLLDRNGLRPARWVMTQDGIITVSSEIGVRRVPPETVVA
                     KGRVGPGQILAIDTVAGRVLHTDEIDDLLKSAHPYKRWIKERSVHIESKLVEEDIDLA
                     HLAPGERNAYMKMFHVTREERDQTLRVLGESGQEATGSMGDDIPMAVLSQQVRSLYDY
                     FRQQFAQVTNPPIDSLREGVVMSLETKLGSERNIFTQTPEHAEQVLLTSPTLSPAKFA
                     KLLQLNHAGFKVATLDLNFPVEVGLEAAVRQLIEQAEQRVRDGVNILVLSDRGITSGK
                     LPVHALMATGAVHHRLIASGLRADTSIVVETATARDPHQLAVLLGYGATAVYPYLGYE
                     VINSLVEDGTLLCDIEEAHKNYRKGINKGLLKILSKMGISTIASYRGAQLFEAIGLHR
                     NLVDLCFSGTVSRIGGADFDDFHVEQQILAAEAWSPRKPLRAGGLHKYVHGQEYHCFN
                     PDVVGTLQQAVQSGDYRTYRKYAELVNERPVATLRDLFALRGDIKPVPLDEVEPIEAI
                     LPRFDSAGMSLGALSPEAHESLATAMNRLGARSNSGEGGEDPARYGTERVSKIKQIAS
                     GRFGVTPHYLVNAEVIQIKIAQGAKPGEGGQLPGGKVNELIARLRYSIPGVTLISPPP
                     HHDIYSIEDLAQLIFDLKQVNPKALISVKLVAEPGVGTIAAGVTKAYADLITISGYDG
                     GTAASPITSIRYAGSPWELGLSEAQQTLRMNHLRDKVRLQTDGGLKSGLDVVKAAMLG
                     AESFGFGTAPMVALGCKYLRICHLNNCATGVATQNHQLRQEHYIGTVEMAMNFFRFVA
                     METREWLARLGVRKLEELVGRSDLLELLPGATAKQRKLDLTPILANQSLPAELPQFCK
                     SLRNMPFDKGELAERMVRDILPAIESKSGGSYHYRVTNCDRTLGARLSGEIARRHGNN
                     GMSDAPVQLLLEGTAGQSLGAFNAGGLHIHLTGEANDYVGKGMAGGQVVIRPHPQSRL
                     ATEKTAIIGNTCLYGATGGKLFAAGRVGERFGVRNSGAQAVVEGAGDHCCEYMTGGII
                     TVLGDTGYNFGAGMTGGFAYVLDLDNTFVDKYNHQLVDINRIRNEFMEGHRNFLRGMI
                     EAYVAETGSGWGQTILDNYVDFVRKFWLIKPKAAELAELLDSVRKSPQ"
     gene            complement(13063..14439)
                     /gene="ffh"
                     /locus_tag="PKI23_06210"
     CDS             complement(13063..14439)
                     /gene="ffh"
                     /locus_tag="PKI23_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNN36565.1"
                     /translation="MFESLADRLSKTLRGITGQARLTDDNIKATLREVRLALLEADVA
                     LPVVKDFIERVRERAVGQEVMRSLTPGQVFVGIVQRELEAVMGERNERLNLAAQPPAV
                     ILMAGLQGAGKTTTVAKLARYLSEREHKKPLLVSVDVYRPAAIKQLQLLADEVGAEFF
                     PSETGQQPLDIVRAAIDQARRKFFDVVIVDTAGRLHVDEAMMNEIKQLHAAINPVETL
                     FVVDAMTGQDAANTAKAFNDALALTGVILTKVDGDARGGAALSVRQITGKPIKFIGVG
                     ERNDALEPFHPDRIASRILGMGDVLSLIEEAERKLDKQKAEQLSKKLQKGKGFDLEDF
                     RDQLQQMRNMGGITGILDKIPGLGGGIPQAARNQVNERLFVQMEAMINSMTPLERRKP
                     ELINGSRKRRITEGSGTQIQDLNRLLKQHKQMQKMMKTMGKKGGMANLMRGMGGRLPP
                     GGMPPMNF"
     gene            14602..15405
                     /gene="ccsA"
                     /locus_tag="PKI23_06215"
     CDS             14602..15405
                     /gene="ccsA"
                     /locus_tag="PKI23_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNN36566.1"
                     /translation="MIQTAFFAIACYSLGALLQWRSFTDRTEIAPRWIRLLGLTGLVA
                     QTLHLRGLILTPLGIDLGLFHVVSAVAASMVLMTLAASLREPMDNLFILILPLAALST
                     LLGAVLKSPYQPFPTPSPGLTLHIISSVLAYSLLAVAAFQALLLAFQNRQLKARHGWK
                     LLRHLPPLETMEKLLFQLIRFGLLLLTLAIVSGALSLENLFAQHLAHKTILSLIAWCI
                     FAALLWGRHRFGWRGSTAVRWTLFGFLALMLAFYGSKLVLELILHRPNP"
     gene            15449..16717
                     /locus_tag="PKI23_06220"
     CDS             15449..16717
                     /locus_tag="PKI23_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HNN36567.1"
                     /translation="MTDLPLSLLLGIMVGLMLVSAFFSAAETGMMALNRYRLKHLDAK
                     GHGGARRTTLLLRRPDRLIGFILIGNNFANNLAASLATILAVRLIGEDNIATAAGIAT
                     AITTFALLIFSEATPKTWAALYPERIAYPASWVLLPLLRLFSPLVWLINTFSNGLLYL
                     FGVKIRQSSPDQLSTEELRTVVLEAGGMQPGRHHSMLLRIFELEELTIRDIMIPRGEV
                     IGIDIEAPLNLIIEQLRSSLHTRLPVYRGELNQVVGILHLRNVARFLTDPNPTHDKLL
                     ALVREPYFVPESTDLQSQLLHFQHEKRRIGIVADEYGEVLGIVTLEDILEEIVGNFTS
                     NIAETEWGISQDDEAGYLIEGSMPLREINRSLNWHLPTDGPRTLNGLILEQMESIPDG
                     NACIRIGAYQVETVQIKEHQIKIARIRLAP"
     gene            16766..17620
                     /locus_tag="PKI23_06225"
     CDS             16766..17620
                     /locus_tag="PKI23_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36568.1"
                     /translation="MPNSDSKPAGPPRLAPEIDEIATYQQRRQSGRPRDKEGQPPPGK
                     SRGLLLVWLVLIALTAAIAGGGFLTVQYLTALQHDIKSLREADLNNIAALTDLQARLG
                     IAGKGTGGAGDLSSRIEQLDAEVRKLIERQKQRDQDLNTQSRQSIDTVQNQMGDLSSR
                     MSKGLTDIDGVNRRIGEQAQRQEAIERQTASLAEQLRSIEAGVKRQDELIARQLTNRL
                     RTVEEQLVTVQQADPGLLNAIERRLRENEAAVAAFDQQRMQLNRSMQELQINMRQLQQ
                     MQMNRPGG"
     gene            17613..>18251
                     /locus_tag="PKI23_06230"
     CDS             17613..>18251
                     /locus_tag="PKI23_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNN36569.1"
                     /translation="MAERPPPRRLDAELRYTPATASKRLLIIHNPVAGRRRQDYLNAV
                     IAALRRQGCELLIRQTSAPGEGERLLRDHPDRFDCIVAAGGDGTLNEVLNGTRGRACR
                     LGLIPLGTSNVLCRELALPTQPDRLAQVLAGDSEVVLHPALANGRRFLLMCGIGFDAW
                     VVNGVDLDLKRRLGKGAYLLTMARMVGRYGRQRYRVRSDDQQWQVGSLIISHA"
BASE COUNT         3294 a         6110 c         5613 g         3234 t
ORIGIN      
        1 agccgccgaa cagcgacgag cgctacaagt tcgacaccta cacgcagcat gtcctccagg
       61 gcttcccgaa taccgagaag tacgagaccg agatcaagcg ccgcttcatc gaggagcctg
      121 caaacaccaa gctgctgatc gtggtgagca agctgctcac cggctttgac gcgccgagct
      181 gcacctacat ctacctcgac aacgcgctgc gcgaccacaa cctgttccag gccatctgcc
      241 gcaccaaccg gctcgacggc gccgacaagg actacgggca catcgtggac ttcaaggagc
      301 tgttcggcga cgtgcaggag gccatcgcgg tctacagctc ggacgagctc gataccgatc
      361 agggcaacgg cggcgataac aacgtccacc tgaaggactg gctcgtcgaa ggccgcagga
      421 agctcgacgc ggcgcacgag gcgctgcgct atctgtgcga gccagtgccg ctgccacgcg
      481 aggtggagca gtacctgcac tacttctgcg gcgacgccgc caacgccaat gcgctcaacg
      541 aaaccgagcc gttgcgggtg gcgtactaca aggccgtggc gctgtttgcg cgcgcctggt
      601 ccgagctggc ccagaacctg gccgaagccg gctacaccga cgccgaggcc gcggcgctgc
      661 gccaggaagt ggagttccac gccgagatcc gcgccgccat caagaagcac tcgggcgagg
      721 agctggacat caagccctac gaggcggaca tgcgccacct catcaatacg tacatccagg
      781 ccgatgcggc cgcgggcctg ggccagctcg gcgatctgtc gctggtcgag ctcatcgtcg
      841 cgaccggcat ccacgacgcc atcgcgcgca agctcaacga gaagggccga ctgtcgaaga
      901 acgccatcgc cgagggcatc atcaacaacc tccgcaagac catcatccgc gagcagctca
      961 ccgacccgcg cttctacgag cagatgtcgc tgctgctgga cgacctgatc cggcagagcc
     1021 gggccgacag tgcggcctac gaggagttcc tgcgcaaggc cgaggacctg gcgaagcggg
     1081 tggccgcgaa gcagccggac gcggacctgc ccgcggcgct gcacggcaag cgtgaggcgg
     1141 cggtcatcta caacaatttg ccgcgaattc ttgcggccgc agacgccggc ttggccgatc
     1201 aggtggccga ggcatcgccg gaatacgcag acgaacgcct ccgcttggcg ctggaggtcg
     1261 accgcgtcat gcgcgagcac gctccggcgg ggtggaaagg tgaccaggca aaggaggcgc
     1321 aggtgctgaa tgcgctgttt ccgctgctga accgtgaccg cgcggcgacg ctggcgctct
     1381 tcgagctcgt caagaaccag ccgggttacg catgaccgag atgctgcact tcggtgatct
     1441 ttcgatcgaa ctgacgcgca aggcggtcaa gcatgctcat ctgtccgtgc atccgcccga
     1501 aggtcgggtg acgctggtcg cgcccatcga cacacgaacg gaagtggcgc gcgcctatgt
     1561 cgtttcaaaa ttgcgctgga ttcgggatca acgcgcgggc tttgccgttc aggcgcgaga
     1621 gccgccgcgc cgcttggtca cccgggagac tcattacctt tggggtcggc gttatctgct
     1681 cgaagtcgag catcgcgacg gtaaaccggt catttcgctc gatcaccggc gcatgcgcat
     1741 ggctgtgcgc ccggacagca gcctggccgt ccgcgcccga gtgctgcacg actggcacaa
     1801 atcactgctt cacgacgtgg tgccggacct gatcgcgaag tgggagccgc ggttgggggt
     1861 gcgggtgcgg gcctatttcc ttcagcgcat gaagaccaaa tggggaagtt gcaaccccag
     1921 ggcagggcac atccgcctga ataccgaact ggtcaagaag cccaaggacc tgctcgaata
     1981 cgtgatcgtg cacgagatgc ttcatctgat cgagccgacg cacagcgagc ggttcctcgg
     2041 gctcatggac aggcactacc cggtgtggcg cgaagcccga gcggagctga acgagctgcc
     2101 gctgtaggtt gtcggccgga gttctggccc atggccaccg gcacaagatg cctattcacc
     2161 gattgttgtt gcaggggggg gtggtgccca ggggcggaat cgaaccaccg acacgaggat
     2221 tttcaatcct ctgctctacc gactgagcta cctgggcgaa ggccggcaat taaaccctct
     2281 gacgccgccg tggtcaatag aaaagctgcc gttaccgctc cggttgcacg tagccctcaa
     2341 ccgcggtgag cgcctcgccg ctcaggaagc gctccatctc ctcctgcaga aatttgcgtg
     2401 acgcgctctc catcatgttc aggcgccgct cgttgatcag ccgggtctgg tgcgccagcc
     2461 actcctgcca cgcctgcttc gacacctgct ggtagatcgc ttcgccacga ggtcccgggt
     2521 agggtggcgc gtcgagcccc tccatttcgc gcttgtattt gcgacagaac accattctcg
     2581 tcatgcggac ctcacggatc agggttgcag ttgcctcagc agccgggtga cgggcgcggc
     2641 aagcccgacc cggcatggtt gctgaatgtt acaccagcgc actccctgat gaagcagggc
     2701 atcgtccgcc agcgccacgt cgatcagctg gggccgcacc cgcagccgca ggtgggtgaa
     2761 gagatgctcg aagtgcggca gcggcgtcac ggccatcacg ctgaagcccc agcgcgcctg
     2821 cacctcgtga tcgatgtcga catcgatcac gctctcggga aaggcccaca atccgcccca
     2881 gacaccggtc gccgggcgct gttcgagcag cagcgactga ctggcattgc gcagcagcag
     2941 ccaggtcagc tcacgcaccg gcaccgatcg gcgtggcctg ggcgtcggca attcggcagt
     3001 gcgcccctgt tgccgggcca cgcactgctg cgcgacaggg caggcatcgc agtggggtcg
     3061 ggcacgggtg cagaccaggg caccaaggtc catcaccgcc tgggtgtagt cggagaatgc
     3121 ggttgccgga gtgtgtgcct cggccagttg ccagagccgc tgctcgacgc tccgttcgcc
     3181 cgaccagccc tcgacaccat gcagacggct cagcagccgc ttgacgttgc cgtcgagaat
     3241 cggcgcgcgc acgcccaagg cgatgctgag aatcgcaccc gctgtcgagc gaccgatgcc
     3301 cggcagtgcc gcaagctgct cgacagtggt gggaaaacag ccctgatgct gctccaccag
     3361 caatcgggca gtgcggtgca ggttgcgcgc ccgcgcgtag tagccaagac cgctccagcg
     3421 atgcagcacc tggtcgagcg gtgccgcagc cagatgttcg agcgcaggaa aagccaccag
     3481 aaaccgttcg aagtagccgg ttgccgtcgc cacctgggtc tgttgcagca tgatctcggc
     3541 gatccagacc cgataggggg tgatctgggt ccgccatggc aaccgctgac ggccatggtc
     3601 gtgataccag gccagaatgc gcgaagtcag caaggtgggc ggcggcacgg atgcagtgtc
     3661 tgctgttttt ttcatgggct ctttcttatg ttaggctttg aaaaacagga aattggtcag
     3721 caatcgtgct cgccatcctg acgtggaaag ggagccgccg cgtgagcaga tcgatcttcg
     3781 caaccgtcgg cgcggcctct gcttcgctgc acccgtcggc ccggcccatg gcagatcagg
     3841 ctttgtgccc tcgccctgtt cgccgacgcc acactccatg aacagcaccg ctcatcacct
     3901 tctgctcaaa agactgctgg tcgtgctggc ggggtggctg gtcctgcatg cgctttcact
     3961 ccaggccgaa ctcctgcaac tggatcccga agtgcgcgac tacccggtca cgggctctct
     4021 gcaggtgttg cgcgaccccc ggcacagatt ctccatcaat gaactgttgc agggcacggc
     4081 acacggccag tggcaacagt tcaccgcatc ttcgcctccc aatttcggct tgacccgcga
     4141 tgcgatctgg ctgcgcctgc ccatcgagca gcgggcgcgc accactcagt ggctgctgga
     4201 gatgagcaac ccgctgctgg accaggtcga cttccatctg gtgcgaaatg gccgggtgct
     4261 ggcgcatttc atcaccggcg accactaccc ctttgtccag cgtccccgtg cgcaccgcca
     4321 cctgctcttt ccgctcgatc tggaagccga aggtcgctac gaaatctggc tgcgcgtcga
     4381 gagcagcagc accatgaacc tggggctgca cctgtggcaa cccgatcaat tctggaaaaa
     4441 agatcagccg ctgctgctcg gctatggctt cttccatggc atgttgatgc tgttctcgct
     4501 ctattacctg ttcatctttg cctggacgcg ggaacggtca cacctctact ttgcgctgtt
     4561 ctcgctctcc agcgcgctca ccgtcctcac cattcatggc ttcggctttc aatacctctg
     4621 gccgaacgcc atcgaatggc aggagcaggc gatcttcttt ctcgtgccga tggcctgcct
     4681 gacgctcggc aagttcgccg gccatctgct gcaactgcgt ggctggcgcc agttgcgccc
     4741 cttgctgttg tcactggctg ggtggttgag cctgctctac atggtggcct cgccgctgct
     4801 gccgccacaa ccgcgcatgg tgctgctcgg tgcactgctg ctctacagct ttggcatcac
     4861 ctttgtgatt tcgctgctgg agctgtttcg cggcaaccgg tcgattcgtt acttcatcct
     4921 cggctggacg ctgttcctgc tctgcgtgct cgtgttcgtg ctcagccgcc tgggctggct
     4981 gccaccgctg cccaatgccg aaaatgccat cgaggtgggc ctggccgggc tgatcatcat
     5041 gctctccttc gccctcaccg cccgtctcaa cgaagagcgg cgcagcaaac tggcagcaca
     5101 gcaggaggcg ctgcaccatg agcaacaact ggtgcgggag cagcagcaca gcctcgacat
     5161 catgcgccag gccagtgccg agctggagca gaacgtggcc cggcgcacgc acgacctcaa
     5221 tgtggccctc aagaagctcg aacatgccaa cgcccaactg caactgctgt cgacggtcga
     5281 cagcctcacc ggcctgaaga accgccgcta tttcgatcag atctaccatt cggagtggcg
     5341 ccgcgccctg cgtgagcaga cgccgctgtc gctgctgctg atcgatgccg accacttcaa
     5401 gcgcatcaac gatcagctgg ggcacccggc cggcgacgaa tgtctgcggc agcttgcgca
     5461 gcgcatctgt tcggcggtgg cacgcccctc cgacctggtc tcgcgctatg gcggagagga
     5521 gttcgccgtg ctgctgccca gcacggcgct gaccggcgcg ctgcagatcg ccgagcggat
     5581 cagggagcag gtgaaggcgg aggccgtcgt ctggcaggag cagccgatct ggctgaccgt
     5641 cagcatcggc gtcgcatcgc tgaaaatcga cagcagcatc acgcccgatc tgatcgtgcg
     5701 ctaggccgat gcggcgctct accgcgccaa gaacgagggg cgcgaccgca cctgctggtc
     5761 gaccgaatcg ggcacgcctc agccctgact ctggtgatag ggcagcgaga gctcatgcac
     5821 cgcatcgacc agcgcggcga catggtccgg ttcgatctgc gggtgaatgc catgccccag
     5881 gttgaagaca tggccattgc cggcaccata gcgggccaga atgtcaccca cttcgtgccg
     5941 aatggcggcc ggtgaggcat agagcaccac cggatcgagg ttgccctgca gcgccacccg
     6001 gtcgcccacc cgcgcacgcg cctcaccgat gtcgaccgtc cagtcgagcc ccagcgcatc
     6061 gcagccggtc gcggcgatcc gctccagcca gagtcccccc tgcttggtga acagaatcac
     6121 cggcacccga ctgccgtcgg gcgcggggtt gaggccggcg acgatccgcg ccatcggccg
     6181 cagcgagaag cgctcatacc agccggtggt cagggtgccg ccccaggtgt cgaagatctg
     6241 caccgcctgc accccggcag cgatctggcc attcagatag tcggtcaccg ccagcgtcag
     6301 cttgtcgagc agcagttcga gcgcttccgg ctgatcgagc agcatccgct tgatggtgcg
     6361 aaaatcgcgg ctcgattgcc cctcgaccat gtaggtggcc agcgtccagg ggctgccgga
     6421 gaagccgatc agcggcaccc gcccggccag ttcgcggcga atcagccgca ccgcgtccat
     6481 cacatagccc agatcgcgct cggcatcggg aatcggcagc gcttcgatgg ccgccggggt
     6541 cgtgaccggc tggcgaaagc gcggcccttc accctcctcg aagtagagcc ccaatcccat
     6601 cgcatccggc acggtcagga tgtcggagaa caggatggcc gcatccagcg caaagcgctc
     6661 cagcggctgc atcgtcacct ccagcgccag ttgcgggctc tggcacaggc tcatgaagtc
     6721 gccggcacgg ctgcgggtgg cgcggtactc cggcagatag cggccggcct ggcgcatcag
     6781 ccagaccggg gtgacatcca ccggctcacg acgcagtgcg cgcagcaggc ggtcattgat
     6841 cagggcggtc atgagcgtgt cttgtacctg tcagcagtgg atggaatcga agcagactgt
     6901 cttgaggagt gcgaatcaga caccgagcgc atcgaggatg ccttcggccg cctgccgtcc
     6961 ctcccagatg gccgtcacca ccagatcgga gccacgcacc atgtcgccgc cagcgaagat
     7021 cttcggattc gcggtctgga actggaaagc gcccggctga cccacctcga cgccgcccca
     7081 actgttgacc gcgatgccat gctcggcaaa ccagggggcg gggctgggac gaaaaccaaa
     7141 ggccagcaac accacatcgg ccggcagaat ctcctcgctg cccggaatcg gttcgggacg
     7201 gcgccggccc ttctcgtcgg gctcaccgag ccgggtctcc accaccttga ctccctcgac
     7261 ccgccactgg ccgacgatct cgaccggttg gcggttgaac aggaagtgga ccccctcctc
     7321 gcgcgcgttc ttcacctcgc gcttcgatcc gggcatgttg gcttcgtcgc ggcggtaggc
     7381 acaggtgacc gacttggccc cctggcgaat ggcgctgcgg ttgcagtcca tcgcagtgtc
     7441 accaccgccc agcaccacca cccgctgccc cttgaggttg atatgggggt actgctccat
     7501 cggcagttgc aggtggtggg agacattgcc gatcagatag ggcagcgcct cgatgacacc
     7561 ggcaatctcc tcgccgggga agcccccttt catggtgtcg taggtgccca tgccgagaaa
     7621 gaccgcgtca tactccgcca gcaactgctc catcgtgatg tcgcgcccca cctcggtgtt
     7681 gaggcggaac tcgatgccca tgtcggtgaa gatctcgcgc cgcagtgcca tcacctgctt
     7741 ttcgagcttg aactcgggga tgccgaaggt cagcagtccg ccgatctcgg gatggcgatc
     7801 aaagaccacc gccttcacgc cgttgcgggt cagcacatcg gcacagccaa ggccagccgg
     7861 ccccgcaccg acgatcgcca cccgcttgcc ggtcggcacc acatgtgaca ggtcgggccg
     7921 ccagcccatc gcaaaagcgg tgtcggtgat gtacttctcg gccgagccga tggtcaccgc
     7981 gccaaacttg gtgttgaggg tacaggcccc ctcacagagc cggtcctgcg ggcagacccg
     8041 accacacacc tctggcaggg tgttggtctc gtggcacagc tccacggcct cgatgatgtt
     8101 gccttcggtg agcagccgca gccagttggg aatgtagttg tggaccggac acttccactc
     8161 gcagtagggg ttgccgcaat cgaggcagcg atgggcctgc tgctcggcct gcttctgctc
     8221 gaaaagatca tagatctcga tgaagttgcg cttgcgcacc cggatcggct tgcgcgacgg
     8281 atcgctgcgc ccgacctgga tgaattgaaa gtcgttgttc aaacggtcac tcatgaccac
     8341 cacctcgtca catcaatggt atcgccgctc attgggggct cttgcgcacg ctgtcgagca
     8401 gctcggccaa ctcggcggcc ttgggtttga tcagccagaa cttgcgcacg aagtcgacat
     8461 agttgtccag gatcgtctga ccccagccac tgccggtttc ggccacatac gcctcgatca
     8521 tgccacgcag gaagttgcgg tggccctcca tgaactcatt gcgaatccgg ttgatgtcga
     8581 ccagctggtg gttgtacttg tcgacgaagg tgttgtcgag atcgagcaca taggcaaaac
     8641 caccggtcat gccggcgccg aagttgtagc cggtgtcacc cagcacggtg atgataccgc
     8701 cggtcatgta ctcgcagcag tggtcaccgg ccccctcgac caccgcctgg gcgccggagt
     8761 tgcgcacacc gaaccgctcg ccgacccgac cggccgcgaa cagcttgccg ccggtggcgc
     8821 catagaggca ggtgttgccg atgatcgcgg tcttctccgt cgccaaccgg ctctgcggat
     8881 gggggcgaat caccacctgg ccaccggcca tccccttgcc gacgtagtcg ttggcctcgc
     8941 cggtcaggtg aatgtgcagt ccaccggcat tgaaagcacc cagactctgt ccggcggttc
     9001 cctccagcag cagctggacc ggggcatcgc tcatgccgtt gttgccatgg cggcgggcaa
     9061 tctcgccgga gagccgcgcc cccagggtgc ggtcgcagtt ggtgacccga tagtggtagc
     9121 tgccgccgct tttgctttcg atggccggca ggatgtcacg caccatccgc tcggccagtt
     9181 cgcccttgtc gaacggcatg ttgcgcagcg acttgcagaa ctgcggcagc tcggccggca
     9241 gcgactggtt ggccaggatt ggcgtcaggt caagcttgcg ctgcttggca gtcgctccgg
     9301 gcagcagctc cagcagatcg ctgcgcccca ccagctcttc gagtttgcgc acgccgagcc
     9361 gcgccagcca ctcccgcgtc tccatcgcga cgaagcggaa gaagttcatc gccatctcga
     9421 cggtgccgat gtagtgctcc tgacgcaact ggtggttctg cgtcgccacc ccggtggcgc
     9481 agttgttgag gtggcagatg cgcaggtatt tgcagccgag cgccaccatc ggcgccgtgc
     9541 cgaaaccgaa actctcggcc cccagcatgg cggccttgac cacgtcgagg ccgctcttga
     9601 gtccgccatc ggtctgcaag cgcaccttgt cacgcaggtg gttcatccgc agggtctgct
     9661 gcgcctcgct cagccccagc tcccatgggg agccggcata gcggatcgag gtgatcggac
     9721 tcgccgcggt gccgccgtca taaccggaga tggtgatgag gtcggcatag gccttggtga
     9781 ccccggcggc aatggtgccg acacctggtt cggccaccag cttgaccgaa atcagcgcct
     9841 tcggattgac ctgcttgaga tcgaagatca gctgcgccag atcctcgatc gaatagatgt
     9901 catggtgcgg cggtggcgag atcagcgtca cgccgggaat cgaatagcgc agccgggcaa
     9961 tcagctcgtt gaccttgccc cccggcaact gtccgccctc acccggcttg gccccctggg
    10021 cgatcttgat ctgaatcacc tcggcgttga ccaggtagtg cggcgtgacg ccaaagcggc
    10081 cggaggcgat ctgcttgatc ttggagaccc gctcggtgcc gtagcgggcc ggatcttcgc
    10141 caccctcgcc cgagttggag cgcgcgccca gccggttcat cgccgtcgcc agcgactcat
    10201 gcgcctcggg cgacagggcg cccaatgaca tgccggcgga gtcgaaccgc ggcagaatcg
    10261 cctcgatcgg ctccacctcg tcgagcggaa ccggcttgat gtcgccgcgc aatgcaaaga
    10321 gatcacgcag cgtcgccacc ggccgctcat tgaccagttc ggcatacttg cggtaagtac
    10381 ggtaatcacc gctctgcacc gcctgctgca gcgtccccac cacatcggga ttgaaacagt
    10441 ggtactcctg accatgcaca tatttgtgca ggccaccggc acgcagcggc ttgcgcggtg
    10501 accaggcctc ggcggccaga atctgctgct caacgtgaaa atcgtcgaaa tcggcgccgc
    10561 cgatgcggct gacggtgcca ctgaagcaga ggtcgaccag attgcggtgc agaccgatcg
    10621 cctcgaacag ttgcgcgccc cggtaggagg cgatggtgga gatgcccatc ttcgagagga
    10681 tcttcagcag ccccttgttg atccccttgc gataattctt gtgcgcctcc tcgatgtcgc
    10741 agagcagggt gccatcctcg accaggctgt tgatcacctc atagccgaga taggggtaga
    10801 ccgccgtggc gccatagccc agcagcaccg ccaactggtg cgggtcgcgc gccgtggcgg
    10861 tctcgacgac gatcgaggtg tcagcgcgca gcccgctcgc aatcagccgg tgatgcactg
    10921 caccggttgc catcagcgca tggaccggca gcttgcccga cgtgatgccg cggtcgctca
    10981 gcaccaggat gttgacgccg tcacgcaccc gttgctcagc ctgttcgatc agttgccgga
    11041 ccgctgcttc gagcccgact tcgaccggaa agttgagatc gagggtggcc accttgaaac
    11101 cggcatggtt gagttgcagc aacttggcga acttggccgg tgacagcgtc ggcgaggtga
    11161 gcagcacctg ttcggcgtgc tccggggtct gggtgaagat gttgcgctcc gaacccagct
    11221 tggtctccag cgacatcacc acgccttcgc gcagcgagtc gatcggcgga ttggtcacct
    11281 gggcaaactg ctgccggaaa tagtcataga gtgaacgcac ctgctgcgac agcaccgcca
    11341 tcggaatgtc gtcgcccatc gaaccggtcg cttcctgccc actctcgccc agcacccgca
    11401 gcgtctgatc acgctcctcg cgggtgacat ggaacatctt catgtaggca ttgcgttcac
    11461 ccggcgccag gtgggcgagg tcgatgtcct cctcgaccag cttcgattcg atgtgaaccg
    11521 agcgctcctt gatccagcgc ttgtaggggt gggccgattt cagcagatca tcgatctcgt
    11581 cggtgtgcag cacccgaccc gccaccgtgt cgatggcgag aatctgtccc ggtccgaccc
    11641 gacccttggc caccaccgtt tcgggcggta cccgccgcac gccgatctcc gaagagaccg
    11701 tgatgatgcc atcctgggtc atcacccagc gcgccgggcg cagaccgttg cgatcgagca
    11761 gacagacggc ggtgctccct tcggtcagca cgatcccggc cggaccatcc caggcctcca
    11821 tcaacatcga gtgatactcg tggaaggcac gcaggtcggt gtcgatggtc tcgacatttt
    11881 cccaggccgg cggaatcatc attcgcaccg cccggtagag atcgaccccg ccggccagca
    11941 gtgtctccag catgttgtcc atcgacgagg agtcggagcc ctcgcggttg accagcggcg
    12001 agatggcggc aatgtcgggc agccagggcg tgctcatgcg cgcctcacgc gcactcaccc
    12061 agttgcggtt gccctggatc gtgttgattt cgccattgtg cgccagcagc cggaagggtt
    12121 gcgccagcgg ccacttcggc agggtgttgg tcgagaaacg ctgatggaag gtgcagatcg
    12181 ccgtctccat gcgcgcatcg tgcagatcgg gatagaagcg cggcagatcg gccggcatca
    12241 tcagcccctt gtagcagagc accttgctcg acaggctggt gatgtaaaac tcctggtcga
    12301 ccggcgccac ggcgcgttcg gccttgcgcc tggcgatgaa cagccggaca ttgaatgtac
    12361 gctcatcgag gccggccggt gcttcgacaa agatctgctc gaagcgcggc atgttgccga
    12421 gcgcgatcgg ccccagcacc gtcggatcgg tcggcaccgg ccgccatccc gtcacgacca
    12481 agtcttcgcc gatcaacgcc tgctccagcg cctgcctggc cgacagcgcg gccgactcct
    12541 gctgcgacag aaacaccatg ccacagctgt acagctcggc cagtggccga ccgaacagtt
    12601 cacccgccac ctcacgcagg aagcgatcgg gcttctgcat caacagacca cagccatccc
    12661 cggtcttgcc atccgcggcg atgccaccac ggtgggtcat gcagttgagc gcatcgatgg
    12721 ccgaccgcag caaggcatgg ctggttcgcc cttcgatctg ggcgatcaga ccaaagccgc
    12781 agttgtcctt gaactcatca gggtggtata gaccggtctg catggaaaac tactctgatc
    12841 aaatgggatg acggacccct ccgcagcgtc aagacgcagg cgcggcagag gcatgtcctg
    12901 agcgaagcga aaggggagat attctaagcc attgccacac attggtgaca agagtcagtc
    12961 gcacccgcta cggatcagcc tgcagagagg ctgtttctgg catgaatcgg tccgaaacgc
    13021 ccggcgtttg cggccgtcga aggatgaaag cgggcaaaaa gcctaaaaat tcatcggcgg
    13081 catgccgccg ggcggcagcc gtccacccat gccgcgcatc aggttggcca tgccgccctt
    13141 cttgcccatg gtcttcatca tcttctgcat ctgcttgtgc tgcttcagca gacggttgag
    13201 atcctggatc tgcgtccctg acccctcggt gatgcgccgc ttgcgcgaac cgttgatcag
    13261 ctccggcttg cgccgctcca gcggcgtcat cgagttgatc atcgcctcca tctgcacgaa
    13321 cagccgctcg ttgacctgat tgcgtgccgc ctggggaatg ccgccaccca gaccgggaat
    13381 cttgtcgaga atgccggtga tgccacccat gttgcgcatc tgctgcaact ggtcacgaaa
    13441 atcctccaga tcgaagccct tgcccttttg cagcttcttg ctgagctgct cggccttctg
    13501 tttgtcgagc ttgcgctcgg cctcctcgat cagcgacagc acatcgccca tgccgaggat
    13561 gcgcgaggcg atccggtccg gatggaacgg ctccagtgca tcattgcgct cgccgacacc
    13621 gatgaacttg atcggcttgc cggtgatctg ccggaccgag agcgccgcgc caccgcgggc
    13681 atcgccatcg accttggtca ggatcacccc ggtcagggcc agcgcatcgt tgaacgcctt
    13741 ggcggtgttg gcggcatcct ggccagtcat cgcgtcgacc acgaagagcg tctcgaccgg
    13801 attgatcgcc gcatgcagct gcttgatctc gttcatcatc gcctcgtcga catgcagccg
    13861 cccggcggtg tcgacgatca ccacatcgaa gaactttcgc cgcgcctggt cgatggcggc
    13921 gcggacgatg tcgagcggct gctggccagt ctcactgggg aagaactcgg ccccgacctc
    13981 gtcggccagc agctgcaact gtttgatcgc ggccggacga tagacgtcga cgctgaccag
    14041 cagcggcttt ttatgttcac gctcggagag atagcgcgcc agtttggcca cggtggtggt
    14101 cttgcccgcg ccctgcaagc ccgccatcag gatgacggcc ggcggttgag cggccagatt
    14161 gagccgctca ttgcgctcac ccatcaccgc ttcgagttcg cgctggacga tgccgacgaa
    14221 aacctggccc ggcgtcaggc tgcgcatcac ctcctggccg accgcccgct cccggacccg
    14281 ttcgatgaag tccttcacca ccggcagcgc cacgtcggct tccagcagcg ccagccgcac
    14341 ctcgcgcagc gtcgccttga tgttgtcatc ggtcagccgg gcctgaccgg tgatgccgcg
    14401 caaggtcttt gaaagccgat ccgccagact ctcgaacatg tccgcctctg caatcgcaat
    14461 aaagtgacaa agggcgatta tagccgcgac ctcgaccgcg tcgcgcaaca aagcggcgct
    14521 attcccagcc tgtggggatg ctcctatact gaccgcgcac gtgccctgcc cgaaaaaacc
    14581 cgactcactg atgagcctct ggtgatccaa accgcctttt ttgccattgc ctgctacagc
    14641 ctgggcgccc tgctgcagtg gcgctccttc accgacagaa ccgagatcgc accacgctgg
    14701 atccgtctgc tggggctcac cggactggtg gcccagaccc tccacctgcg cgggctgatc
    14761 ctgacgccgc tcggcatcga tctggggctg tttcatgtgg tcagcgccgt cgctgcatcg
    14821 atggtgctga tgacactcgc cgccagtctg cgcgagccga tggacaatct gttcatcctg
    14881 attctgccac tggcagcgct cagcacactg ctgggcgcgg tgctgaagag tccttaccag
    14941 ccctttccga caccctcccc gggcctgact ctgcacatca tcagctcggt gctggcctac
    15001 agcctgctgg ccgttgctgc gtttcaggcg ctgctgctgg cgtttcagaa ccggcaattg
    15061 aaggcacgtc atggctggaa gctgctgcgc cacctgccgc cactcgagac gatggagaag
    15121 ctgctgttcc aactgatccg cttcggcctg ctgctgctga ccctcgccat cgtcagtggt
    15181 gcgctttcgc tggagaatct gttcgcccag catctggccc acaagaccat cctctcgctg
    15241 atcgcctggt gcattttcgc tgcgctattg tgggggcgac atcggttcgg ctggcgcggc
    15301 agcaccgccg tgcgctggac gctcttcggc tttctggcgt tgatgctggc cttctatggc
    15361 agcaaactgg tgctggagct gattctgcac cgtccgaacc cttgaccctc ggaccggcac
    15421 tgccgggaac aacagaaaaa ggaagccctt gaccgaccta ccgctctccc tgctgctcgg
    15481 catcatggtt ggcctgatgc tggtctcggc attcttctcg gccgccgaaa ccgggatgat
    15541 ggcgctcaac cgctaccgcc tgaagcatct cgacgccaag ggccatggcg gcgcgcgccg
    15601 caccacactg ctgctgcggc ggcccgatcg gctgatcggg ttcatcctga tcggcaacaa
    15661 cttcgccaac aatctggccg cctcgctggc gaccatcctg gcggtccggc tgatcggtga
    15721 ggacaacatt gccaccgctg ccggcatcgc cacggccatc accaccttcg cgctgctgat
    15781 cttcagcgag gccactccca agacctgggc cgcgctctac ccggaacgga tcgcctaccc
    15841 cgcctcctgg gtgctgctgc cactgctgcg gctgttttcg ccgctggtct ggctgatcaa
    15901 taccttctcc aatggcctgc tttacctttt tggcgtcaag atcagacaga gcagccccga
    15961 ccagctctcg accgaggagc tgcgcaccgt ggtgctcgaa gccggcggca tgcagcccgg
    16021 ccgtcaccac agcatgctgc tgcgcatctt cgaactggag gagctgacca tccgcgacat
    16081 catgattccg cgcggagagg tgatcggcat cgacatcgag gccccgctca acctgatcat
    16141 cgagcaactg cgcagcagcc tgcacacccg cctgccggtc tatcgcggcg agctcaacca
    16201 ggtggtgggg attctgcatc tgcgcaacgt cgcgcgcttt ctgaccgatc ccaacccgac
    16261 ccacgacaag ctgctggcgc tggtgcgcga gccctacttc gtgccggaaa gcaccgacct
    16321 gcagagccag ctgctgcact tccagcatga gaagcgccgc atcggcatcg tcgccgacga
    16381 gtatggcgag gtgctcggca tcgtcacact cgaggacatc ctcgaggaga tcgtcggcaa
    16441 cttcaccagc aacatcgccg aaaccgagtg gggcatcagt caggatgatg aagcgggcta
    16501 cctgatcgag ggctcgatgc cgctgcgcga aatcaaccgc tcgctcaact ggcacctgcc
    16561 caccgatggc ccgcgcacgc tgaacggcct gattctggag cagatggagt cgattcccga
    16621 cggcaatgcc tgcatccgca tcggtgccta ccaggtcgaa acggtgcaga tcaaggagca
    16681 ccagatcaag atcgcccgca tccggttggc gccatgacgc cactgtgatt gactagcctc
    16741 cgctttacca ccggaccccc tgcccatgcc caacagtgac tccaagcccg cgggaccgcc
    16801 acgcctcgca cccgagatcg atgagatcgc cacctatcag cagcgccgcc agagcggccg
    16861 gccacgtgac aaggaggggc agcccccccc tggaaaaagt cgcggactgc tgctggtctg
    16921 gctggtgctg atcgcgctga ccgccgccat cgccggcggc ggctttctca ccgtgcagta
    16981 cctgaccgca ctgcagcatg acatcaagtc gctgcgggag gccgatctga acaacatcgc
    17041 ggcgctgacc gacctgcagg cgcgcctcgg cattgccggc aagggtaccg gcggcgccgg
    17101 cgacctctcc agccgcatcg agcagctcga cgccgaggtg cgcaaactga tcgaaaggca
    17161 gaagcagcgc gatcaggatc tgaacaccca gagcagacag tcgatcgaca cggtgcagaa
    17221 tcagatgggc gacctctcca gccgcatgag caaggggctg accgacatcg acggggtcaa
    17281 ccgccgcatc ggcgaacagg cacagcgaca ggaggccatc gagcggcaga ccgccagtct
    17341 ggcggaacag ctcaggagca tcgaggccgg cgtcaagcgc caggatgagc tgatcgcccg
    17401 gcaactgacc aatcggctgc gcaccgtcga agagcaactg gtcacggtgc agcaggccga
    17461 ccccggcctg ctgaacgcga tcgaaaggcg gctgcgcgaa aacgaggccg ccgtggccgc
    17521 cttcgatcag cagcggatgc agctcaaccg ctcgatgcag gagttgcaga tcaacatgcg
    17581 ccagcttcag cagatgcaga tgaaccgccc cggtggctga gcggccacca ccacgacgcc
    17641 tggatgctga gctccggtac acgcccgcca ccgccagcaa gcgactgctg atcatccaca
    17701 acccggtcgc cggtcgccgc cgtcaggact atctgaatgc cgtgatcgcc gcgctgcgtc
    17761 ggcagggctg cgaactgctg atccggcaga ccagcgcccc cggcgaaggg gagcggctgc
    17821 tgcgtgacca cccggaccgc ttcgactgca tcgttgccgc cggcggtgac ggcaccctca
    17881 acgaagtgct gaatggcacg cgcgggcgcg cctgccgcct gggtctgatt ccgctcggca
    17941 cctccaatgt gctctgccgc gagctggcgt tgccgaccca gcccgaccgg ctggcccagg
    18001 tgttggccgg tgacagcgag gtcgtgctgc atccggccct ggccaatggc cggcgctttc
    18061 tgctgatgtg cggcatcggc ttcgatgcct gggtggtgaa tggcgtcgac ctcgacctca
    18121 aacgccggct gggcaaaggt gcctacctgc tgacgatggc cagaatggtc ggccgctatg
    18181 gccgacagcg ctatcgggtg cgcagcgacg accagcagtg gcaggtcggc tcgctgatca
    18241 tcagccatgc c
//
LOCUS       DAORCQ010000043        17405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2033_length_17405_cov_5.602421, whole genome shotgun sequence.
ACCESSION   DAORCQ010000043 DAORCQ010000000
VERSION     DAORCQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 17405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17405
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_17405_cov_5.602421"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PKI23_06235"
     CDS             <1..141
                     /locus_tag="PKI23_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNN36570.1"
                     /translation="DAIEAAVSSVLDQGCRTADIALPGEQPIGTAEMGSAVVAALRGQ
                     SS"
     gene            138..914
                     /locus_tag="PKI23_06240"
     CDS             138..914
                     /locus_tag="PKI23_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNN36571.1"
                     /translation="MNLHPDETQRVQQSWNAAEYQRHAAFVPALAHEVVRLLNPRPGE
                     EILDLGCGDGSLTAQMQNLGCSVTGIDSSQEMVAAARERGVDAHLVDGHSLTFSERFD
                     AVFSNAALHWLIRPESVIAGVHRALKPAGRFVGEFGGRGNLAALVEAMRKTFAENPQF
                     GSFDLPWFFPSVDEYRHMLENADFEVRQIELIPRPTPLESGIEKWLEIFADGITRQLS
                     LTQKSTFMESVKRRLVDTLYTDETGWVADYVRLRFAAFKR"
     gene            991..2013
                     /locus_tag="PKI23_06245"
     CDS             991..2013
                     /locus_tag="PKI23_06245"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNN36572.1"
                     /translation="MSKLYDVAVVGATGAVGETMISILESRNFPVGQLYPLASSRSAG
                     KSINFRGKAVAITDLAQFDFGKAQIGLFSAGGSVSAEYAPKAGAAGCVVIDNTSHFRY
                     HDEIPLVVPEVNPEAIAGYTRHHIIANPNCSTIQMLVALKPIHDAVGITRINVATYQA
                     VSGTGREAINELREQTGSLLAGKPAEARVYPRQIAFNVLPHIDQFQENGYTKEEMKMV
                     WETHKILGDASIAVNPTCVRVPVFHGHSEAVHIETRDKITAERARALLAVAPGVVLLD
                     ERAPGGYPTPALDAAGQDPVFVGRIREDISHPHGLDLWVVSDNLRKGAALNSVQIAEL
                     LISGYL"
     gene            2163..4772
                     /locus_tag="PKI23_06250"
     CDS             2163..4772
                     /locus_tag="PKI23_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNN36573.1"
                     /translation="MRKPFSYMAAGLVVMGQAGTAAALGLGEIELQSALNQPLSANIR
                     LLQADGLASDEILITLATPAEFERAGIDRPALLSELRFVIATDAEGRRVIRVSSREPM
                     REPFLDFLLEVQWPQGKMMREFTLLLDPPGYVMKQPVTPPAVRTAPVVMPAASAPAAA
                     PTPRPTPSEPAPASEGGGLHRVGSAGNTLWAIAKAVRPSSEVTLQQTMMALRDLNPQA
                     FVDGDINRLKRGALLQVPSLDQIRQRSASAAAQAVAVASQQRRQAHEGVPAPALTTTA
                     PAPAVAAKAPDAELKLVAPSAPETKPAQAAAGNSKKESATTEELNQKLALTLENLDRL
                     IEENKELKARQDEIGQQLQTMQKLLNLKDEQLAGLQAKLAAEQSVEAKPAAEAAAPQD
                     EQGLLARLLAEPLYLAASAAAVGVTTLLAVLGLRRRAAQRQAVDAYPADDFMFDDSSV
                     PVAAASVAMVESASTPEPAPSMPVAPQSSGPRTEPVAPRAPETADPLTEADIYLAYGR
                     HEPAIALLTGAIEREPDRSDFWLKLLEIHAERKDRTAFDAAKQKIDALGDPLAIQRAA
                     ELKMTLGGAAVTAEPELFAFTPAEPVEGGIDFSRIALDEPPVAVTAQIDVAPAAAMAP
                     LDFDLAGSLEVPPSTFAPTAPVAEPTGRLPELEFESGLFAPQAEPPAVVESAAGEAGE
                     IDFEFDFGSLEPPAPLAPGEVVRAEDFDLATPLADVVASVDDEDMDLDTLHMRMREEM
                     AARLAAAQPTPPVGEEEDLDSLHRRAREEMQQLLASDLAGSAPGVPAVEVPHPVSEPL
                     FRPHAESDGGFEDETFNLLAGTDENATRLDLARAYLDMGNHDGARELIDEVLLEGDPQ
                     QQDEARALLFRLG"
     gene            4802..5620
                     /gene="truA"
                     /locus_tag="PKI23_06255"
     CDS             4802..5620
                     /gene="truA"
                     /locus_tag="PKI23_06255"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003212671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNN36574.1"
                     /translation="MSEAGSTRIALGIEYDGHPYRGWQSQSHCPGQTVQERLQAALGR
                     VADHPVELICAGRTDAAVHATGQVVHFTSHTARPLKAWVLGTNSHLDGSIGVTWAKVV
                     PDDFHARFSALSRRYRYLIYNHPLRPVLQRQQLSWCHQPLSVEPMQQAAQALVGEHDF
                     NAYRAVACQARSAVRRVDFITVGRIGRLVMIDIQANGFLHHMVRNIAGVLIRIGAGEA
                     EPEWAQQVLESRDRTRGGVTAPPQGLYLVAVGYPQRFDLPRLPLGPLWMQDAGG"
     gene            5664..6308
                     /locus_tag="PKI23_06260"
     CDS             5664..6308
                     /locus_tag="PKI23_06260"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNN36575.1"
                     /translation="MRTRIKICGITRIEDAHCAVEAGADAIGLVFHPASPRALSLDAV
                     ADLIAQLPPFVSTVGLLVNADRGLLHEVVTLGLHLLQFHGDESPAECEQAGVPYIKAV
                     RMAPLFDLEREVARYPGAAAILVDSYDPQRYGGTGQTFDWGRLPSVLSKPLILAGGLT
                     PDNVAAAIRQVGPYAVDVSGGVERAKGVKDHGKIRAFVKAVARGDAVRGGAVEP"
     gene            6305..7510
                     /gene="trpB"
                     /locus_tag="PKI23_06265"
     CDS             6305..7510
                     /gene="trpB"
                     /locus_tag="PKI23_06265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNN36576.1"
                     /translation="MSEQNRALPDRQGHFGIYGGRFVAETLMHALDQLERAYFTLRED
                     GQFWAAFDHDLAHYVGRPSPLYHAERLTGILGGAQIYLKREDLNHTGAHKINNTIGQA
                     LLAKHMGKRRIIAETGAGQHGVATATVAARLGLECQIYMGAEDVARQSLNVYRMKLLG
                     AQVIPVTSGSRTLKDAMNEALRDWATEVDHTYYIIGTVAGPHPYPLLVRDFQAVIGRE
                     ARQQMLERTGRLPDALVACVGGGSNAIGLFHPFIDDAEVAMHGVEAAGDGIATGRHAA
                     PLCAGRVGVLHGNRTYLIEDDAGQIIETHSVSAGLDYPGVGPEHAWLKDSGRVRYDAV
                     TDAEALAAFRQLTQVEGIMPALESSHALAFVNKLAPTLRSDQTIVVCLSGRGDKDIHT
                     VAALDHITL"
     gene            7536..8351
                     /gene="trpA"
                     /locus_tag="PKI23_06270"
     CDS             7536..8351
                     /gene="trpA"
                     /locus_tag="PKI23_06270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNN36577.1"
                     /translation="MSRITPRLAALKAQRRTALIPYLTAGDPDLGTTLALMHTAVAAG
                     ADILEIGVPFSDPCADGPVIQAASERALCQGVRLVDVLALVSQFRASDDETPVVLMGY
                     LNPVEIFGYGRFADAAASAGVDGLILVDLPPEEAAQFEPLLAQAGIDLIYLVAPTTTP
                     ERARMIARHARGYLYYVSLKGVTGAGHLDVADVAARVTQLREFTTLPVAVGFGIKDGA
                     SAAAVARHADGVVVGSALVERIGRLGHAPAQASAAVAALIGELRQALDHSGAA"
     gene            8389..9270
                     /gene="accD"
                     /locus_tag="PKI23_06275"
     CDS             8389..9270
                     /gene="accD"
                     /locus_tag="PKI23_06275"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNN36578.1"
                     /translation="MSWLDKIIPSIGRSTGKEADKRSSVPEGLWINCPKCKAMLYQPE
                     LERNLDVCPKCDHHIRIGARRRIDIFLDRDDREEIAANVLPGDPLKVKDTKRYKDRLS
                     SAQKETHEKEALVVIKGRLESLPVVVAAFEFRFMGGSMGSVVGEKFVRAVDVALQQRI
                     PLICFSTSGGARMQESLLSLMQMAKTAAALERLKQQGIPYVSVMIDPIYGGVAASLSM
                     LGDINIAEPYALVGFAGPNVIRQTVRVTLPEGFQRSEFLLEHGAIDMIVHRHQLRATV
                     ARLLGKLTQGAAASPAA"
     gene            9282..10565
                     /gene="folC"
                     /locus_tag="PKI23_06280"
     CDS             9282..10565
                     /gene="folC"
                     /locus_tag="PKI23_06280"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNN36579.1"
                     /translation="MAPALGTLAQWLSYLEQLHPQLIELGLERVSEVARRLGVARPAA
                     RVITVAGTNGKGSTVRFLELLLSAAGQRVGCYTSPHLLHYRERIRIAGEEASDPAICA
                     AFAQIEQARGDISLTYFEFGTLAALLLFAQQPLDILLLEVGLGGRLDAVNLVDADLAI
                     ITTIDYDHQEWLGHDLDSIGAEKAGILRAGRPAILGMNPPPPRLLQLAQSLAQPLLRR
                     GVDFDARVAGSQWHYQESSAAVPFTLGPLPLPRLPLDSALCALRAVRLLGHDLPVPRI
                     AELMNTATLAGRFQQLAGRVPIIVDVAHNPQSAALLASRLAAEPCAGRTHAVVAIMAD
                     KAIEPMLQHLLPRVERWYPAEARTPRSATSRQLVELLRGLGAAVEQGFDSVTAAIDAA
                     EAAVQPGDRILIFGSFYTVAEALEGWQARQAEPMV"
     gene            10598..11140
                     /locus_tag="PKI23_06285"
     CDS             10598..11140
                     /locus_tag="PKI23_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNN36580.1"
                     /translation="MGAEQDEKKIRLELRRRLLGALVLVALVVIFLPGLLEQTRYQPD
                     RARFQIPPPPERAQWAPGTPLVNEAELREKLAAGPPSTVGPVPEQDGPPSTLDQQGLP
                     IAWVAQLGDHADSQSAAQWLARVQALGFDAHLQPLQRPNGTRYLLYAGPFWHREQAEA
                     AAQRLRNELKLEPKVVRYQP"
     gene            11230..12126
                     /gene="htpX"
                     /locus_tag="PKI23_06290"
     CDS             11230..12126
                     /gene="htpX"
                     /locus_tag="PKI23_06290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNN36581.1"
                     /translation="MKRILLFVLTNLAVIVLLGITTRLLGVDRFLTQGGINMPALFGF
                     AAVFGMGGSFISLLMSKWMAKMSTGARVIEQPGDATERWLLETVRAQAQRASIAMPEV
                     AIYDAAEPNAFATGPSRNNALVAVSSGLLHTMNRDEVEAILGHEVSHIANGDMVTLTL
                     IQGVVNTFVIAFARIIGFMVDRFLSGDRDEESSAPGMAYWISSMVAELILGFLASAIV
                     LWFSRQREFRADSGGAQLAGRDKMISALERLQTLHEPAQLPSQMAAFGVRGGTAGALG
                     RLFMSHPPIEERIAALRSGMSA"
     gene            12189..12803
                     /locus_tag="PKI23_06295"
     CDS             12189..12803
                     /locus_tag="PKI23_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNN36582.1"
                     /translation="MSAVPDIHLDLGCGPMPRNPYRRGRLYGIDLRSGMTLRSGAADG
                     ELLSANLVLEPIPFADDFFGSVSAYDFLEHVPRQLIDPRSGVLRNPFVELMSEIWRVL
                     APGGRLLASTPAYPRPEAFQDPTHVNIITRDTHDYFCGDPAPAAMYGFRGGFRVVEVG
                     WSVEKNSRDPLQPRWRKRLRNWNHRLFKQGLSHFAWELEAIKRP"
     gene            12952..13641
                     /locus_tag="PKI23_06300"
     CDS             12952..13641
                     /locus_tag="PKI23_06300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNN36583.1"
                     /translation="MASTTQSAGRRVTLPAEPFDLDFDPQRTALIIIDMQRDFVLPGG
                     FGEKLGNDTSLLLAAVEPISRVLEKARQREMLVIHTREGHRPDLSDCPPAKLNRGGKV
                     FIGTPGPMGRILVRGEQGHDIIHQLYPIDGEPVIDKPGKGSFHATDLQQILFDRAIST
                     LIVCGVTLEVCVHTTVREANDRGYECVVLSDCVASYFPEFKRVGLEMITAQGGIFGWV
                     SSGDALIAALG"
     gene            13654..14484
                     /locus_tag="PKI23_06305"
     CDS             13654..14484
                     /locus_tag="PKI23_06305"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HNN36584.1"
                     /translation="MLPALCIGHRVELARPQAGCVHSCFEGAVNLLIEGNLWSLLDGA
                     RSDLPFGIRLATGTAPGRLELRVGQPVQAKAGYLGVGPLTIDCRMAMRWWPRGWSAPT
                     AGLAARLDRVERIAAPRAWRDSAEMAVAVTEALLRRGPGAAAMLSGTVGRVVGRGPGL
                     TPAGDDVLVGILTVLGSTAARPMGASLRARLVAALRPWLGTTGDISRQLLQQVMQGSP
                     GRALHELGQAVVEGMDEAAMQSALERLLDSGGSSGADGCIGLIAACRRLWLPAERAIA
                     "
     gene            14481..16046
                     /gene="fdrA"
                     /locus_tag="PKI23_06310"
     CDS             14481..16046
                     /gene="fdrA"
                     /locus_tag="PKI23_06310"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HNN36585.1"
                     /translation="MNSASRYYANRYQDSVALMKLSAQVMAMPGVELAAVVMASAGNI
                     DTLAAAGLGRFEVRPNDIIVVVKGSEAGCAAALERADALLTAPAESAPQEEGSVPLPI
                     TSLHGAVRRDPGHNLALVSVPGDYAAAEAMKALRLGMDVMLFSDNVPPAAELTLKHYA
                     RRHDLLVMGPDCGTAIINGIPLGFANVVRRGPIGAVGASGTGLQEVTSRIHRLGSGIS
                     QAIGTGGHDLSAEIGGISMLQGLQALTDDPATRVIVLVSKPPAPAVAEVVLAAAEACS
                     KPVVVIFLGVDPRSLTRQGVHGASTLAEAADLAVTLAQGEMPAAAPPSIPVELLHRFG
                     ALCRSMAPTQRHLRALFCGGTFCFEAQLLHAAAGIAVASNVSTAGNPQLEQARIGPGH
                     SMVDLGDDLFTRGRPHPMIDPVLRDSRLLQEADDPTTAVILFDVVLGHGAAADPLTGL
                     LGAIEQARSRALAAGRRLLFIAHVCGTDQDPQNRSAVVAALRAAEVFVADSSAEAALW
                     SVAVLRDCLGGAA"
     gene            16043..>17405
                     /locus_tag="PKI23_06315"
     CDS             16043..>17405
                     /locus_tag="PKI23_06315"
                     /inference="COORDINATES: protein motif:HMM:NF018275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HNN36586.1"
                     /translation="MKSLFQQPLKVANVGLPAFADNIAAAGGAVTQLRWAPPAGADAD
                     LGWRLAMLIGDPRVEQANRTAYARYLAARPRLVDLQLARDAIAALGGGERRILHAGPP
                     IDWARMCGPQQGAIAGAMLYEGWADSLEAAARMAAAGEVALEPCHDHGAVGPMAGVIS
                     PSMPLWVVENDDHGNRAYCNLNEGLGKVLRFGANTPEVIARLRWLGTDFFTTLRRAVR
                     ALNGGELKPLMAQALQMGDELHNRNLAASGLLFKRLALALLDSGLDSGAIQRALGFIA
                     GNDHFFLNLSMAACKVMLDAAHGVPGSSLVTAMARNGVNFGIRLSGTGTQWFEAPANP
                     VQGLYFAGYGVEDAAADLGDSAITETAGLGGFAIAAAPAIVQFVGGTAADASANSRRM
                     LEITLGSNPAFSLPPLDFAGTPAGIDARLVVDSGILPIINTGIAHRQAGVGQIGAGIT
                     TAPL"
BASE COUNT         2813 a         5586 c         5971 g         3035 t
ORIGIN      
        1 gatgccatcg aggccgccgt ctccagcgtg ctcgatcaag gctgtcgcac cgccgacatc
       61 gccctgccgg gggagcagcc gatcggcacc gccgaaatgg gcagcgcggt ggtggccgcg
      121 ctgcgcgggc agtcgtcatg aacctccacc cagatgagac gcagagggtg cagcagagct
      181 ggaatgccgc tgaataccaa agacatgccg cctttgttcc ggcgctggcg catgaggtcg
      241 tcaggctgtt gaatccacga cctggagagg agattctcga cctcggctgt ggtgatggca
      301 gcctgacggc gcagatgcag aatctgggct gttcggtgac cggcatcgac agcagccagg
      361 agatggtggc agcggcaaga gagcgcgggg tcgatgccca tctggtcgat ggtcacagcc
      421 tgacgttcag tgagcggttc gatgcggtct tcagcaacgc ggcgttgcac tggctgatcc
      481 gacccgagag cgtcattgcc ggtgtccatc gggcgctcaa gcccgccggg cgtttcgtcg
      541 gggagtttgg tggtcgcgga aaccttgccg ccctggtgga ggcaatgaga aagacctttg
      601 cagaaaatcc gcagtttggc agcttcgatc tgccctggtt ctttccgtct gttgacgaat
      661 accgacacat gttggaaaac gccgatttcg aagtgcgcca gatcgaactg attcccaggc
      721 cgacaccgct ggagagcggt atcgagaagt ggctggagat ctttgccgat gggatcacgc
      781 gtcagctctc cttgacgcaa aaatccacct tcatggaatc ggtgaagcgc aggcttgtcg
      841 acaccctgta tacggatgag acgggctggg tggcggatta tgtccggttg cggttcgcgg
      901 ctttcaagcg atgaactgcc gctcgagtgg cgcgctgctt ggcgcatggg tttctggagt
      961 tcgtgataaa tttctgttcg gagtcaaacg atgagcaagc tttacgatgt ggccgtcgtc
     1021 ggcgccaccg gcgcggtggg cgagacgatg atctcgattc tcgagtcgcg caactttccg
     1081 gtcgggcagc tctatccgct ggccagcagc cgctcggcgg gtaaaagcat caactttcgc
     1141 ggcaaggccg ttgcaatcac cgatctggcg cagttcgact tcggcaaggc gcagatcggc
     1201 ctgttctcgg ctggcggttc ggtgtcggcc gagtatgcgc ccaaggccgg cgccgccggt
     1261 tgcgtggtga tcgacaacac ctcccacttc cgctaccacg acgagattcc gctggtggtg
     1321 cccgaggtga accccgaagc gatcgccggt tacacccggc accacatcat cgccaacccc
     1381 aactgttcga ccatccagat gctggttgcg ctgaagccga ttcatgatgc cgtgggcatc
     1441 acgcggatca atgtcgccac ctaccaggcg gtgtcgggca ccggccgcga ggcgatcaac
     1501 gaactgcgcg aacagaccgg cagcctgctg gcaggcaagc cggccgaggc ccgggtctat
     1561 ccccggcaga tcgccttcaa cgtgctgccg cacatcgatc agtttcagga gaatggctac
     1621 accaaggaag agatgaagat ggtctgggag acccacaaga tcctcggcga tgcctcgatt
     1681 gcggtcaatc cgacctgcgt gcgggtgccg gtgtttcatg gccattccga agcggtgcat
     1741 atcgagaccc gcgacaagat cactgccgaa cgggcgcgtg cactgctggc cgtggcaccg
     1801 ggcgtggtgc tgctggatga gcgggcgccg ggtggctatc cgacgccggc gctcgacgct
     1861 gcagggcaag acccggtgtt cgtcggccgg atccgggagg acatctccca tccgcacgga
     1921 ctcgacctct gggtggtctc cgacaacctg cgcaaggggg cggcgctcaa cagcgtgcag
     1981 attgcggagt tgctgatcag cggctatcta taactggagc ttttgctgaa agcgtcggat
     2041 ttcagcttga aaatggactc acagcgaagg cagacgggta caatcacgcc gaactggagc
     2101 cggggaggcc gcctgatgcg cggattttcc ggtctcttcg ccacactgca agggagcagc
     2161 tgatgcgcaa acccttttct tacatggccg ctggcctggt cgtcatgggt caggccggta
     2221 ccgcagccgc gttggggctg ggtgagatcg agctgcagtc ggcgctgaat cagccgctgt
     2281 cggccaacat tcgcctgttg caggccgatg gactggccag tgacgagatc ctgatcacgc
     2341 tggccacccc ggccgaattc gagcgggccg gcatcgaccg ccccgcgctg ctctccgaac
     2401 tgcgcttcgt catcgccacc gatgccgagg ggcggcgggt catccgggtg tcgagccgtg
     2461 aaccgatgcg ggagcccttc ctcgactttc tgctcgaagt gcagtggccg cagggcaaga
     2521 tgatgcgtga gttcacgctg ctgctcgatc cgcccggcta tgtgatgaag cagccggtga
     2581 cgcccccggc cgtgcggacg gcgccggtgg tcatgccggc cgcatccgct cccgcagcgg
     2641 ctccgacacc acgaccgacc ccaagcgagc ctgcccctgc cagcgaggga ggtggtctgc
     2701 accgggtggg cagcgctggc aacaccttgt gggcgattgc caaggcggtt cgcccttcca
     2761 gtgaggtgac tctccagcag accatgatgg cgctcagaga tctcaatccg caggccttcg
     2821 tcgatggtga catcaatcgg ctcaagcgtg gcgccctgct gcaggtgccc tcgctcgatc
     2881 agatcaggca gcgcagtgcc agcgcggcgg cccaggccgt ggcagtggca tcgcagcagc
     2941 gacgacaggc gcacgaaggc gtgccggctc cggcattgac gacgacggca ccagcaccgg
     3001 cagttgcggc caaggcgccc gatgccgaat tgaagctggt ggcacccagc gcgcctgaaa
     3061 ccaaaccggc ccaggcggca gcgggcaaca gcaagaagga gtcggccacg accgaagagc
     3121 tgaaccagaa gctggcgctg acactggaga acctcgatcg tctgatcgaa gagaacaagg
     3181 agctgaaggc gcgacaggat gagattggcc agcagttgca gacgatgcag aagctgctca
     3241 atctgaaaga tgaacaactg gcaggactgc aggcgaagct ggcggccgag cagtcggttg
     3301 aagccaaacc ggcagccgag gctgctgccc cacaggacga acagggtctg ctggcccgac
     3361 tgctggccga gccgctctac ctggcggcat ccgccgctgc ggtgggagtg acgaccctgc
     3421 tggccgtact ggggttacgg cgacgcgcgg cgcaacgcca ggccgtcgat gcctatccgg
     3481 cggatgactt catgttcgat gacagctcgg tgccggtggc tgcggccagt gtcgcgatgg
     3541 tggagtcggc gtcgacgcca gagcctgcgc cctccatgcc ggttgccccg cagtcgagcg
     3601 gaccgcgcac cgaacctgtc gctccccgag cacccgagac cgccgacccg ctgaccgagg
     3661 cggacatcta cctggcctat ggccgccatg agccggccat tgcgctgctg accggtgcca
     3721 tcgagcgcga gccggatcgc agcgacttct ggctcaagct gctggagatc catgccgagc
     3781 gcaaggaccg cacggccttc gacgcagcca agcagaagat cgacgcgctg ggtgacccgc
     3841 tggcgatcca gcgcgccgcc gaattgaaga tgaccctggg tggcgccgcc gtgactgcgg
     3901 agccggagct ctttgccttc acgccggctg aaccggtaga aggtggcatc gacttcagcc
     3961 ggattgcgct cgatgagccg cccgtggccg tgacggcaca gatcgatgtg gcgccggcag
     4021 cggccatggc cccgctcgat tttgatctcg ctggctcact ggaggtgccg ccctccacct
     4081 tcgcgcccac tgcaccggtt gccgagccaa ccggaagatt gccggagctg gagttcgagt
     4141 ccgggctgtt tgcacctcag gcggagccgc ctgccgttgt cgaatccgct gcgggtgagg
     4201 caggcgagat cgacttcgag ttcgatttcg gttcgctcga accgcctgcg ccactcgccc
     4261 ccggcgaggt ggtgcgggcg gaagatttcg acctggcgac gccgctggcc gatgtcgtcg
     4321 ccagtgtcga tgacgaagac atggacctcg acaccctgca catgcggatg cgcgaagaga
     4381 tggcggccag gctggccgcc gcgcaaccga cgccaccggt gggtgaggag gaggatctcg
     4441 actcgctgca ccgccgtgcg cgcgaagaga tgcagcaact gctggcgagc gatctcgccg
     4501 gctcggcgcc gggcgttcct gccgtggagg tgccgcatcc ggtgagcgag ccgctgttcc
     4561 gtccgcacgc cgagtcggac ggcgggttcg aggatgagac cttcaacctg ctggccggca
     4621 ccgacgagaa tgccacccgg ctcgatctgg cccgcgccta cctcgacatg ggcaaccatg
     4681 atggcgcccg ggaactgatc gacgaggtgc tgctcgaggg cgatccgcag cagcaggatg
     4741 aggctcgggc actgctgttc cggctcggct gagcggggtg atgtgagctt tgaccgaccc
     4801 gatcagtgag gccggatcga cgcgcatcgc gctcggtatc gagtatgacg gacatcccta
     4861 ccgcggctgg cagtcgcagt cccactgtcc cggacagacg gttcaggagc ggctgcaggc
     4921 cgcactcggt cgggtggccg accatccggt cgaactgatc tgcgccggcc ggaccgatgc
     4981 cgcggtccac gccaccggcc aggtggttca cttcaccagc cacaccgcgc ggccgctgaa
     5041 ggcctgggtg ctgggcacca acagccatct cgatggttcg atcggcgtga cctgggccaa
     5101 ggtggtgccc gacgactttc atgcccgctt ctcggccctg agccgccgct accgctacct
     5161 gatctacaac catcccttgc gcccggtgct ccagcgccag cagctgagct ggtgtcatca
     5221 gccgctgtca gtcgagccga tgcagcaggc ggcgcaggcg ttggtggggg agcatgactt
     5281 caatgcctac cgtgccgtgg cctgtcaggc gcgcagcgcg gtacggcggg tcgatttcat
     5341 caccgtgggc cgcattggtc gtctggtgat gatcgacatc caggccaacg gctttttgca
     5401 ccacatggtg cgcaacattg ccggggtgct gatcaggatc ggggctggcg aggcagagcc
     5461 tgagtgggcg cagcaggtgc tcgagagccg tgaccgcacc cggggcggcg tgacggcgcc
     5521 gccacagggg ctctatctgg tggcggtcgg ctatccgcag cgcttcgacc tgccccgatt
     5581 gccgctcggt ccactctgga tgcaggatgc tgggggttga tcggctgtta taatgccgcc
     5641 ccttttcgat ctatggccga gccatgcgga cacgcatcaa aatctgcggc atcacccgca
     5701 tcgaggatgc ccactgtgcg gtggaggccg gcgccgacgc catcgggctg gtgttccatc
     5761 cggccagccc ccgcgcgctg tcgctcgatg ccgttgctga tctgattgcc cagcttcccc
     5821 cctttgtcag cacggtcggt ctgctggtca atgccgatcg cggattgttg cacgaggtgg
     5881 tgacactcgg actgcatctg ctgcaattcc acggcgatga gtcgcccgca gagtgcgagc
     5941 aggctggagt gccctacatc aaggcggtgc ggatggcgcc gctgttcgat ctggagcgcg
     6001 aagtggctcg ttatccgggt gccgcggcca ttctggtcga cagctacgac cctcagcgct
     6061 acggcggcac cggtcagacg tttgactggg gacggctgcc ttcagtcctg agcaagccgt
     6121 tgatcctggc ggggggactc acgccggaca atgtcgccgc cgcaatccgg caggtggggc
     6181 cctatgccgt cgatgtcagt ggcggtgtcg aaagggccaa gggggtcaag gaccatggta
     6241 agattcgcgc tttcgtcaaa gcggtcgcaa ggggtgatgc agtgcgcggt ggagcggtag
     6301 aaccatgagt gaacagaatc gggctctgcc cgaccggcag ggccacttcg gcatctatgg
     6361 cggccggttt gtcgccgaaa ccctgatgca tgcgctcgat caactggagc gcgcctactt
     6421 cacgctgcgt gaagatggcc agttctgggc cgccttcgac cacgatctgg cccactatgt
     6481 cggccgcccc tcgccgctct accatgccga gcggctgacc ggcattctgg gtggtgcaca
     6541 gatctacctc aagcgcgaag acctcaacca caccggcgcg cacaagatca acaacaccat
     6601 cggtcaggcg ctgctggcca agcacatggg caagcgacgc atcatcgccg aaaccggcgc
     6661 cggccagcat ggcgtggcca ccgccacggt cgccgcccgg ctgggactgg agtgccagat
     6721 ctacatgggc gccgaggatg tcgcgcggca gtcgctcaac gtctaccgca tgaagctgct
     6781 gggcgcgcag gtgattccgg tcacctccgg ttcgcgcacc ctcaaagatg cgatgaacga
     6841 ggcgctgcgt gactgggcca ccgaggtcga tcacacctac tacatcatcg gcacggtggc
     6901 ggggccgcat ccctatccgc tgctggtgcg tgactttcag gcggtgatcg gccgcgaagc
     6961 ccggcagcag atgctggagc gtaccggtcg gctgcccgat gccctggtcg cctgtgtcgg
     7021 cgggggatcg aatgcgatcg ggctgttcca cccctttatc gacgatgccg aggtggcgat
     7081 gcatggtgtc gaggcggccg gtgatggcat tgcgactggt cgccacgccg cgccgctctg
     7141 cgccggccgg gtcggcgtac tgcatggcaa ccgcacctac ctgatcgagg atgatgccgg
     7201 ccagatcatc gagacccact cggtctccgc cggtctcgac tatcccggcg tcggccccga
     7261 acatgcctgg ctgaaggaca gcggccgggt gcgctatgac gcggtcaccg atgccgaggc
     7321 gctcgccgcc ttccgccagc tgacccaggt cgagggcatc atgccggcac tcgagagcag
     7381 ccatgcgctg gccttcgtca acaagctggc gccaacgctg cgctccgacc agacgatcgt
     7441 ggtctgcctc tccgggcgtg gcgacaagga catccacacg gtggccgcac tcgaccacat
     7501 caccctttag cggatcccct gcagcgagcc ctccgatgag ccgaatcacc ccgcgactgg
     7561 ctgcgctgaa ggcgcagcgg cgcaccgccc tgatccccta cctgaccgca ggcgaccccg
     7621 acctcggtac cacgctggcg ctgatgcaca ccgcagtggc ggccggtgcc gacattctgg
     7681 agatcggcgt gcccttttcc gatccctgtg ccgatggtcc agtcattcag gctgcctccg
     7741 agcgcgccct gtgccaggga gtgcgtctgg tcgatgtgct ggcgctggtc agccagttcc
     7801 gcgccagcga tgatgaaacg ccggtggtgc tgatgggcta cctcaatcca gtggagattt
     7861 ttggctacgg ccgctttgcc gacgcggcgg ccagtgccgg cgtcgatggc ctgattctgg
     7921 tcgatctgcc gccggaagag gcggcccagt tcgaaccgtt gctggcacag gccggcatcg
     7981 atctgatcta tctggtggcg ccgaccacca cgccggagcg ggcacgcatg attgcccgcc
     8041 atgcgcgcgg ttatctctac tatgtctcgc tgaagggggt gaccggtgcc ggtcacctcg
     8101 acgtggccga cgtggccgcc agggtgacgc agttgcgcga attcaccacg ctgccggtgg
     8161 cggtcggctt tggcatcaag gatggcgcca gcgcagcggc ggtggccaga catgccgatg
     8221 gcgtggtggt gggcagcgca ctggtcgagc gtatcggccg tctgggccat gcgccggcgc
     8281 aggcgagtgc cgcagtggcg gcgctgatcg gcgaactgag gcaggcgctc gatcacagtg
     8341 gcgcagcctg atcttttggc aataaaaaca taagagtttc atcacccaat gagctggctg
     8401 gacaagatca tcccgtcgat tggccgttcc actggcaagg aggccgacaa gcgcagctcg
     8461 gttcccgaag ggctgtggat caactgtccc aagtgcaagg cgatgctcta ccagccagag
     8521 ctggagcgca acctcgatgt ctgcccgaag tgcgaccacc atatccgcat cggtgcccgt
     8581 cggcgcatcg acatttttct cgaccgggat gaccgcgagg agatcgccgc caatgtgctg
     8641 ccgggtgacc cgctcaaggt gaaggacacc aagcgctaca aggatcgcct ctccagcgcg
     8701 cagaaagaga cccatgagaa agaggcgctg gtggtgatca aggggcggct cgagagcctg
     8761 ccagtggtgg tggcggcctt cgagttccgc ttcatgggcg gatcgatggg ctcggtggtg
     8821 ggcgagaagt tcgtccgcgc agtcgatgtc gccctgcagc agcgcattcc gctgatctgc
     8881 ttttcaacca gtggtggcgc gcggatgcag gagtcgctgc tgtcgctgat gcagatggcc
     8941 aagaccgccg cggcactgga gcgactgaag cagcagggca tcccctatgt ctcggtgatg
     9001 atcgatccga tctacggcgg ggtggcggcc agcctgtcga tgctgggcga catcaacatc
     9061 gccgagccct acgcgctggt cggtttcgcc gggcccaacg tgattcgcca gaccgtccgc
     9121 gtgacgctgc cggaaggttt ccagcgcagc gaattcctgc tcgaacatgg cgccatcgac
     9181 atgatcgtgc atcgacatca gctgcgcgcc acggtcgccc ggctgctggg caagctgacc
     9241 cagggcgcgg cggcttcgcc agccgcatga gtgcgcgggc ggtggcgcca gcgttgggta
     9301 cgcttgcgca gtggctgagc tatctcgaac agctccatcc acagctgatc gaactgggcc
     9361 tcgaacgggt cagcgaggtg gcgcggcgat tgggtgtcgc gcgccctgcg gcgcgggtga
     9421 tcaccgtcgc cggcaccaat ggcaagggtt cgaccgtgcg ctttctggag ctgctgctga
     9481 gcgcggccgg gcagcgggtc ggctgctaca cctcacccca tctgctgcat tatcgcgagc
     9541 gcatccgcat cgccggcgaa gaggccagcg acccggcgat ctgcgccgcg ttcgcgcaga
     9601 tcgagcaggc gcgtggcgac atctcgctca cctatttcga gttcggtacc ctggccgcgc
     9661 tgctgctgtt cgcgcagcag ccgctcgaca tcctgctgct ggaggtgggg ctgggcgggc
     9721 gtctcgatgc ggtcaacctg gtcgatgccg atctggccat catcaccacc atcgactacg
     9781 accaccagga gtggctcggc catgacctgg acagcatcgg tgccgagaag gccggcattc
     9841 tgcgtgccgg gcgcccggcg atcctcggca tgaatccgcc gccaccgcgg ctgctgcaat
     9901 tggcgcagtc gctggcgcag ccgctgctgc ggcgcggtgt cgatttcgat gcccgggttg
     9961 ccggcagcca gtggcactat caggagtcga gcgcggcggt gcccttcact ctcggcccgt
    10021 tgccgctgcc acggttgccg ctcgactcgg cgctctgcgc actgcgtgcc gtgcgacttc
    10081 tgggacatga tctgccggtg ccgcgcatcg ccgagctgat gaacacggcg acgctggccg
    10141 gccgcttcca gcagttggcc ggccgggtgc cgatcatcgt cgatgtggcc cacaatccgc
    10201 agagtgcggc gctgctggcg agccggctcg ctgccgagcc ctgtgccggg cgcacccatg
    10261 cagtggtggc gatcatggcc gacaaggcga tcgagccgat gctgcaacac ctgctgcccc
    10321 gggtggagcg ctggtatccg gctgaagcgc ggactccccg cagtgcaacc agcaggcagt
    10381 tggtcgaact gctgcgtggg ctcggcgctg cggtcgagca ggggttcgac agtgtcacgg
    10441 cggccatcga cgccgccgaa gcggcggtgc agccggggga tcgcattctg atttttggct
    10501 ccttctatac cgtggccgaa gccctcgaag ggtggcaggc gcggcaggcg gaaccgatgg
    10561 tgtgagatgg aagcagagtg atttcggcag gaacgagatg ggcgctgaac aggacgaaaa
    10621 aaagatacgg ctcgaactgc ggcgtcgtct gctgggcgcg ttggtgctgg tcgcgctggt
    10681 ggtgatcttt ctgcccgggc tgctggagca gacccgctac cagcccgatc gggcgcggtt
    10741 tcagattccg cccccgcccg agcgcgcgca gtgggcgccg gggacgcctc tcgtcaacga
    10801 agccgaactg cgcgagaagc tcgccgccgg accaccctcg accgtcgggc cggtgcccga
    10861 gcaggatggg ccgccgtcga cgctcgatca gcagggattg ccgatcgcct gggttgcgca
    10921 actaggcgat catgcggatt cgcagagtgc cgcgcagtgg ctggcgcggg tgcaggcgct
    10981 cggtttcgat gcccatctgc aaccgctgca acggcccaat ggcacccgtt atctgctcta
    11041 tgcggggccc ttttggcacc gggagcaggc cgaggcggct gcccagcggt tgcgcaatga
    11101 actgaaactg gagcccaaag tggtgcgcta tcagccctga tggttcactt gacggccaca
    11161 ctgcactgct tatcatcgcc ggcggttcgc tgattccgtt acgaattttc atcaccagag
    11221 acccttgcca tgaaacgcat cctgctgttc gttctgacca acctggcggt catcgtgctg
    11281 ctgggcatca ccacccggct gcttggagtc gaccgctttc tgacccaggg tggcatcaac
    11341 atgccggcgc tgttcggctt cgccgccgtg ttcggcatgg gcggctcatt catttcgctg
    11401 ctgatgtcga agtggatggc caagatgtcg accggtgccc gggtgatcga gcagcccggc
    11461 gacgccaccg agcgctggct gctggagacc gttcgcgcgc aggcgcagcg tgccagcatc
    11521 gcgatgccgg aggtggcgat ctacgatgcc gccgaaccca acgcctttgc caccggcccc
    11581 agccgcaaca atgcgctggt ggcggtcagc agcgggctgc tgcacacgat gaatcgcgac
    11641 gaggtcgagg cgattctggg acatgaggtg agccacatcg ccaatggcga catggtgacg
    11701 ctgacgctga tccagggggt ggtcaacacc ttcgtcatcg ccttcgcccg catcatcggc
    11761 ttcatggtcg atcgctttct ctccggcgac cgcgacgagg agtcctcggc accgggcatg
    11821 gcctactgga tcagcagcat ggtggccgag ctgatcctcg gttttctggc cagcgccatc
    11881 gtgctctggt tcagtcggca gcgggagttc cgcgccgaca gtggtggcgc ccaactggcc
    11941 ggccgcgaca agatgatctc ggcgctggag cggctgcaga cgctgcacga gccggcgcag
    12001 ctgccgagtc agatggccgc ctttggcgtg cgtggaggca cggccggcgc actgggccgg
    12061 ctgttcatgt cgcatccacc gatcgaagag cgcatcgctg cattgcgctc cggcatgtcg
    12121 gcctgaccct tccgctgtgg cgcgcaccct cttggcagcg cgctcctttc ctgctggacc
    12181 ctgacccgat gagcgcagtt cccgacatcc acctcgatct gggctgtggt ccgatgccgc
    12241 gcaatcccta ccgccgcggc cggctttacg gcatcgacct gcgcagcggg atgacgctgc
    12301 gcagcggtgc ggccgacgga gaactgctga gcgccaacct ggtgctggag ccgatcccgt
    12361 ttgccgacga tttcttcggc tcggtctcgg cctatgactt tctggagcat gtaccgcggc
    12421 agttgatcga cccgcgctcc ggcgtgctgc gcaacccctt cgtcgagctg atgagcgaaa
    12481 tctggcgggt gctggcgccg ggtggccgac tgctcgcctc gacgcccgcc tatccgcggc
    12541 cggaagcttt tcaggatccg acccacgtca acatcatcac ccgcgacacc cacgactact
    12601 tctgcggtga cccggcgccg gcggccatgt atggtttcag gggcggcttt cgcgtcgtcg
    12661 aggtgggctg gagcgtcgaa aagaacagcc gcgacccgct gcagccacgc tggcgcaaac
    12721 ggctgcgcaa ctggaaccat cggctgttca agcagggcct ttcgcatttc gcctgggagc
    12781 tggaggcgat caagcggccc tgaccgcgac ggtctgcgcg gcagggtgca atgccctgaa
    12841 gcagaaaaca tcgctcgggt cgcacaaggc aagcttcctg catgattttt ttcaggcaag
    12901 catgggctgc ccattggtcc ggtttttttc tccaggcaga gggagcgtgc aatggcatcc
    12961 acgacacaga gcgctggccg gcgggtgacg ctgccggcgg agccgttcga tctcgacttc
    13021 gatccgcaac gcacggcgct gatcatcatc gacatgcagc gcgatttcgt tttgcccggc
    13081 ggctttggcg agaagctcgg caacgacacc tcgctgctgc tggcggcggt ggagccgatc
    13141 tcccgcgttc tggagaaggc gcggcagcgg gagatgctgg tgatccacac gcgggagggg
    13201 caccggcccg atctgtcgga ctgtccgccg gccaagctca accgcggtgg caaggtgttc
    13261 atcggcacgc cgggaccgat ggggcgcatt ctggtgcgcg gcgagcaggg ccatgacatc
    13321 atccaccagc tctatccgat cgacggtgag ccggtgatcg acaagccggg caagggctcc
    13381 tttcatgcca ccgatctgca gcagattctg ttcgatcgcg ccatcagcac actgatcgtc
    13441 tgcggtgtga ccctcgaggt ctgtgtccac accacggtgc gcgaggccaa cgaccgcggc
    13501 tatgagtgtg tcgtgctctc cgactgtgtc gcctcctact ttcccgagtt caagcgggtc
    13561 gggctggaga tgatcactgc ccagggcggc atcttcggtt gggtctccag cggcgatgcg
    13621 ctgatcgctg cgctcggttg agcggggcgg tccatgctgc cagccctctg catcggccac
    13681 cgtgtggaac tggcccgacc ccaggccggc tgcgtccatt catgcttcga aggggccgtc
    13741 aatctgctga tcgaggggaa tctttggagc ctgctcgatg gcgcgcgcag tgatctgccg
    13801 tttggcattc gtctggcgac cggcaccgca cccggtcgac tggaattgcg ggtggggcag
    13861 ccggttcagg cgaaagccgg ctatctgggt gttggaccgc tgaccatcga ctgccgcatg
    13921 gccatgcgct ggtggccgag gggctggtcg gcgccgacgg cgggcttggc ggcgcggctc
    13981 gatcgggtcg agcggatcgc cgcgccgcgc gcctggcgcg attccgcaga gatggccgtc
    14041 gccgtgaccg aggcgctgct gcggcgcgga ccgggcgcgg cggccatgct ctccggcacg
    14101 gtgggtcggg tcgtgggacg tggaccgggg ctgacgccgg ccggagatga cgtgctggtc
    14161 ggcatcctca ccgtgctcgg cagcacagcg gccaggccga tgggtgcgtc gctgcgggcc
    14221 cggctggtcg cggcgctgcg gccgtggctc gggacgaccg gcgacatcag tcgacagctg
    14281 ctgcaacagg tgatgcaggg ctcaccgggc cgtgcgctgc atgagctggg gcaggcggtg
    14341 gtcgaaggca tggacgaagc ggcgatgcag agtgcgctgg agcggctgct cgacagcggc
    14401 ggcagctccg gtgccgacgg ctgcatcggt ctgatcgccg cctgccggcg gttgtggctg
    14461 ccggccgagc gggcaatcgc atgaacagcg cttcgcgtta ctacgccaac cgctaccagg
    14521 attcggtggc gttgatgaag ctctccgccc aggtgatggc gatgcccggc gtcgaactcg
    14581 ctgcggtggt gatggcctcg gccggcaaca tcgacaccct ggcggcggct ggtctgggcc
    14641 ggttcgaggt gcggcccaat gacatcatcg tcgtggtgaa gggcagcgaa gcgggttgcg
    14701 ctgcggcgct cgagcgggcc gatgcactgc tgacggcgcc tgccgaatcg gcgccacagg
    14761 aggagggcag cgtgccgctg ccgatcacca gcctgcacgg ggcagtgcgt cgtgatccgg
    14821 gccacaatct ggcgctggtg tcggtgcccg gcgattacgc cgcggccgag gcgatgaagg
    14881 cgctgcgtct gggcatggat gtgatgctct tcagcgacaa cgtcccgccc gcggccgaac
    14941 tgacactgaa acactatgcg cgacggcacg atctgctggt gatggggccc gactgcggca
    15001 ccgccatcat caacggcatt ccgctcggct ttgccaatgt ggtgcgccgc gggccgatcg
    15061 gcgcggtcgg cgcttcgggc accgggttgc aggaggtgac gtcacgcatc catcggttgg
    15121 gttcgggcat ctcgcaggcg atcggcaccg gtggccatga tctgtcggcc gagatcggcg
    15181 gcatctcgat gttgcagggg ttgcaggcac tgacggatga cccggccacc cgcgtgatcg
    15241 tgctggtctc caaaccaccg gcgccggcgg tggccgaggt ggtgctcgcc gccgccgagg
    15301 cctgcagcaa accggtcgtc gtcatctttc tcggcgtcga cccgcgttcg ctcacccgcc
    15361 agggggtgca tggcgccagc acgctggccg aggcggccga cctggccgtc accctggcgc
    15421 agggcgagat gccggcagca gcgccgccat cgatcccggt cgagctgctg catcggttcg
    15481 gggcactctg ccggtcgatg gcaccgacac agcgccatct gcgtgcactc ttctgcggtg
    15541 gcaccttctg tttcgaagcg cagctgctgc atgcggcggc tggcatcgcg gtcgcttcca
    15601 atgtgtcgac tgcgggcaat ccacagttgg agcaggcccg gatcggcccc ggccacagca
    15661 tggttgacct gggtgatgac ctgttcaccc ggggtcgccc ccatccgatg atcgacccgg
    15721 tgctgcgcga cagtcggctg ctgcaggagg cggacgatcc gacgacggca gtgattctgt
    15781 tcgatgtggt gctcggccat ggcgcggcgg ccgatccact gacgggcctg ctcggcgcca
    15841 tcgaacaggc ccggagcagg gcgctggcgg ccggtcggcg gctgctgttc atcgcccatg
    15901 tctgcggcac cgaccaggat ccgcagaacc gatcggcggt ggtggcggca ctgcgtgcag
    15961 cggaggtctt cgtggccgac tccagtgccg aggcggcgct ctggtcggtg gccgttctac
    16021 gcgactgtct ggggggtgct gcatgaagtc actgttccag cagccgctga aggtggcgaa
    16081 cgtcggcttg ccggcgttcg ccgacaacat cgccgccgcc ggcggtgcgg tcacccagtt
    16141 gcgctgggcg ccgccggccg gcgcggatgc cgacctcggc tggcggctgg ccatgctgat
    16201 cggcgatccg cgcgtcgagc aggccaaccg caccgcctat gcgcgttatc tcgcggcgcg
    16261 gccgcggctg gtcgatctgc aactggcgcg cgatgccatt gccgcgctgg gcggcgggga
    16321 gcggcgcatc ctccatgccg gtccgccgat cgactgggca cggatgtgcg gcccgcagca
    16381 gggggcgatt gccggggcga tgctctacga aggctgggcc gattcgctgg aggcggccgc
    16441 acgaatggcc gcagccggcg aggtggccct cgaaccctgc catgaccatg gggcggtggg
    16501 gccgatggcg ggcgtgattt cgccgtcgat gccgctctgg gtggtggaga atgacgacca
    16561 tggcaaccgt gcctactgca acctcaacga agggctgggc aaggtgctgc gctttggggc
    16621 caacacgccg gaggtgatcg cgcggctgcg ctggctcggc accgactttt tcaccacgct
    16681 gcgcagggcc gtgcgggcgc tgaatggtgg tgagctgaag ccgctgatgg cgcaggcatt
    16741 gcaaatgggc gatgaactgc acaaccgcaa cctggccgca tccggtctgc tgttcaagcg
    16801 gctggcgctg gcgctgctcg acagcggcct cgacagcggc gcgatccagc gcgccctcgg
    16861 cttcatcgcc ggcaacgacc acttctttct caacctgtcg atggcggcct gcaaggtgat
    16921 gctggatgcg gcgcacgggg tgcccggcag cagcctggtg accgcgatgg cgcgcaatgg
    16981 cgtcaacttc ggcattcgtc tgagtggcac cggaacacag tggtttgagg cgccggccaa
    17041 tccggtacag gggctctatt tcgccggcta tggcgtcgag gatgccgctg ccgacctcgg
    17101 cgactccgcc atcaccgaga ccgccgggct cggcggcttc gcgatagccg ccgcgccggc
    17161 gatcgtccag ttcgtcggcg gcaccgcggc cgatgccagc gccaacagtc gacggatgct
    17221 ggagatcacg ctgggcagca acccggcctt cagcctgccg ccactcgatt tcgccggtac
    17281 accggccggc atcgatgccc ggctggtggt cgactccggc atcctgccga tcatcaacac
    17341 cggcatcgct caccgtcagg ccggcgtcgg ccagatcggt gccggcatca ccactgcgcc
    17401 gctgg
//
LOCUS       DAORCQ010000044        16718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2153_length_16718_cov_5.981576, whole genome shotgun sequence.
ACCESSION   DAORCQ010000044 DAORCQ010000000
VERSION     DAORCQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_16718_cov_5.981576"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2679
                     /locus_tag="PKI23_06320"
     CDS             <1..2679
                     /locus_tag="PKI23_06320"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNN36587.1"
                     /translation="QYQQQVLDSVEAYFRACHELPSPSVAFTATTERLWGRGSPYNPL
                     SGFPPDMPYFCLRVPTGGGKTWLAAKSVQLANTHLLRTEHSVILWLVPSKPIREQTLR
                     ALRDRLHPYHAALREAGPITVMDLDEAKSVTRATLDTSTTVIVATRQAFQVEDEECRK
                     VYQSSGALMHHFDNLSPTQRDGLLSEGAGAERTTPCSLANVLRLRRPFVVVDEAHNNR
                     TELAFDMLARFRPSGVLELTATPDLERTPSNVLHSVCAAELKAEEMIKLPVVLETEPN
                     WQQCLADAIGRREALHRLADEARRAGADYLRPVVLIQSEPRRAGVETLDFERVRQELI
                     TNHGIPAREIVVATGEERGLEQIDTNYKLGIADPDCPVKFVITQKALAEGWDCPFAYI
                     LVSMASLSSATAVEQLLGRVLRQPNARHFDDPLLNRSYAFVVSRNFAETAGALRDRLV
                     AGAGFERREVAQFVTAARAEQARIDLDGHAGRAVMRPVVVVLPEKPDLKRLPQPVRDK
                     LSWDGNLNTLTISTPLTEDETDTLTAAVTSEAAATAIAEAAEISRTTAIEFFQTPAER
                     GERFAVPQLALRVQGALQLFDDPELLDYPWALSTCDAAPTTAELAMLGASMKVSEGGE
                     IDVDDGGRLVSRFLPELQRDLGLVCPPEQWDELRLATWLCRNLPEPSLTHASKQAFVA
                     GWLTELLRRDDFPLARASLQKFLIRNLLEARIRQLRRQAVGRAFQQTLFGDDVGQRVA
                     VSDVYAFEFAPHAYAPARDYDGRFGHFDFRRHYYGRIGDFDSREEFDCACWLDMQAQR
                     GRIAFWVRNLVRREGSSFFLQKADGRFYPDFLCQLPGRAGESGPILAVEYKGADRWSA
                     ADDDRLIGGLWASLSDGRCRFVMVTDRGWERIEAQW"
     gene            2698..5340
                     /locus_tag="PKI23_06325"
     CDS             2698..5340
                     /locus_tag="PKI23_06325"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNN36588.1"
                     /translation="MGEPLWHLLGVDEAAQRLDSDRLTGLTADEALRRRQLHGPNELR
                     EAKRRGPLRIFLDQFADFMILVLLAAALVAGLIGDWHDSLVIVVIVLLNALLGFVQEY
                     RAERAMAALRRLAAPQARVQRAGGLLSVPASELVPGDRVLLEAGNVVPADLRLIEVNQ
                     LRTDEAALTGESQPVDKQAAALSEAELPLGDRHNMAYRGTQVVHGRAAGLVVATGMAT
                     ELGRIAMLLQQQLALKTPLQQRLIRFGQRLALACILLCVLIFAMGVWRGEPPLLMFLT
                     AVSLAVAAIPEALPAVITIALALGARSMVGQRVLIRRLPAVETLGSVSYICSDKTGTL
                     TQNRMTLESLWLAGSWYGRDALTTAPAPLRDALLRAVALNNDARRDAAGGWVGDPTEV
                     ALHEAAETFGHGAAALTVSAPRIGEIPFDAERKRMTTLHQQGAALLACTKGAPESVLA
                     CCIGLQSEAGRVALDQQAVLAAAEAMAAQGLRVLAVACRDWDALPERLDSETVEQSLL
                     LLGLVGLIDPPRPEAREAVQWCHRAGITPVMITGDHPATALAIARRLGIAGATDRAVT
                     GPMLATLDPEQLGQLVTEVRVYARVAPEQKIDIVRALQARGAFVAMTGDGVNDAPALK
                     RADIGVAMGQGGTEVACEAADMVLLDDNFASIVAAVREGRRIHDNIRRFVKYAVTTNS
                     AEIWVLFLAQCFALPVPLLPIHILWINLLTDGLPGIALTREPAERDIMRRPPRPPNES
                     LFAHGLWQHMLWAGLLMALLTLALQSWALHNDIAHWQSMVFTLLTLTQMAHVLAIRSE
                     RESLFTQGLASNLPLLGAVLLTLALQLAVLYLPPLQSIFRTAPLALDELLLCVGLAAL
                     LWVAVEIEKWLRRRGAMQRTATGG"
     gene            complement(5359..6183)
                     /locus_tag="PKI23_06330"
     CDS             complement(5359..6183)
                     /locus_tag="PKI23_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNN36589.1"
                     /translation="MSEIFGFDAFSPREIAQRIEQVGVAKARLPLRSMWMLGMLAGAF
                     IGLGALYYTLVAADASLGFAARQLLGGVTFSLGLLLVTVAGAELFTGNNLLVLAWADG
                     RISAHELLRNWVVVCLANFVGAAALALLVFLSRHPEMNQGAIAEQTVKIAAAKTLLPP
                     ATAFFRGMLCNVLVCMAVWMAMAGRTIVDKAVAILFPISAFVAAGFEHSIANMYFIPL
                     AMLIQHFGGGTATQTVITWGGFFHNLLPVLAGNIIGGSVLVGLVYHTIYPRPSDSA"
     gene            complement(6199..7332)
                     /locus_tag="PKI23_06335"
     CDS             complement(6199..7332)
                     /locus_tag="PKI23_06335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36590.1"
                     /translation="MIKMEGAIEFTEEQGMISDSAVSFFREQYPMATVRQLLDDERGY
                     TTDGWRSIGELGWLGMMIPEEFGGAGLSAKELVSVVEPMGRHLVGTPFIATTLAGQLL
                     LKAGSEAQKSHLLPKLASGAAIGSVALFEPHGAWEPDQLRATAQRSGNGFVLSGSKAF
                     VLDAAVADLVIVACQLDGAPALFCLSGPQLQGRLRRETVIDETRRSHRLQLDGLEVGS
                     DAQLTGGDALAALKWCHALGALLHTAESAGGANSALLLTVDYLKTRKQFGQLIGSYQA
                     LKHPTVDAMIAVEHSRSHLYYAASLFNGYDGGESEVAVRMAKVHSNESFYFASDRAIQ
                     FHGGMGFTHECNAGLHFRRAQWNKFSFGDTLHHRRHLRRLLMP"
     gene            complement(7361..8569)
                     /locus_tag="PKI23_06340"
     CDS             complement(7361..8569)
                     /locus_tag="PKI23_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36591.1"
                     /translation="MTTPEPKAHQEFRREVAGWLERNRPAKPDFILPQSFMEVDTQQQ
                     FDYLRDWQRKVYEAGYLGMAWPKEYGGGGQPQILQDIATQEMTRANVPFMVNAIGLSW
                     AGPTILHLGSEAHKKQFIKNILNAEEIWCQGFSEPDHGSDLGNAQCRAERDGDHFVVN
                     GAKIWTSLGVYARHMILLARSIPNTSSKYAGLSFFLCPMQTPGIEVIPIKKITGEYGF
                     NQTVFTNARIHESTLVGEEGKGWLVAMAVLAFERGAAGGQAGGNMSTVFSTQDVIEMS
                     RLIERDGRPALEDPLVQDKLLNFLIDQRSIALTSQRARIEKLLTPERPAATMMMGKLF
                     GSEFMRRLAYFAVELQGGRANLYMGDPEAVQGGEWQRAAMNAYSATIGGGTSEVQHNI
                     LGERVLDLPK"
     gene            complement(8633..9511)
                     /locus_tag="PKI23_06345"
     CDS             complement(8633..9511)
                     /locus_tag="PKI23_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN36592.1"
                     /translation="MQPFKQGKVTANGLEFTFLEQGEGPLVLCMHGFPDTARSFRHQL
                     PALAQAGYRAVAPFMRGYAPTAAAANGNYQTAALGQDVLALIEALGAQQAVVIGHDWG
                     STAASAAALFAPEKVSKLITLALPYGGMGTALLNNWAQQKRSWYIFFFQTALADLAVP
                     LEDFAFIERLWRDWSPGWDFPAEEMRALKQTLAQPGVLAAALGYYRCTMNPERHDPLL
                     APLQARIGREPIQVPTLNIHGALDGCIGAEFSQNIDALFPAGLTNRVIADGGHFLHQE
                     QPEQVNRLILDFLQGT"
     gene            complement(9562..10104)
                     /gene="folE"
                     /locus_tag="PKI23_06350"
     CDS             complement(9562..10104)
                     /gene="folE"
                     /locus_tag="PKI23_06350"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNN36593.1"
                     /translation="MKEAYQKLLESIGEDLNRDGLRDTPERAARAFAFLNRGYHQDID
                     QIINNAIFESESNEMVLVQDIELYSLCEHHLLPFIGKCHVAYIPSGKILGLSKFARIV
                     DMYARRLQVQENLTRQIADTIQKVTQAQGVAVIIEAQHMCMMMRGVEKQNSRMKTSVM
                     LGQFQSNPSTRSELLSLISS"
     gene            complement(10178..10753)
                     /locus_tag="PKI23_06355"
     CDS             complement(10178..10753)
                     /locus_tag="PKI23_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNN36594.1"
                     /translation="MRKNRTPPAPGETLATEERALFQQATADVIRTPAAGCAPIPSKT
                     GQFRPRRSPSPPSDDDARQPAEAAPRPCSGEEPVLFQRPGIQPRQLLQLKRGKFRTEA
                     VLDLHGLRQRQAEIELRAFIDQSWQEGLRTLLIIHGKGHGSASGTAVLKSWLVAELPR
                     FPQLLAFCSALPRDGGTGALYLRLRQQPPTL"
     gene            complement(10872..11303)
                     /locus_tag="PKI23_06360"
     CDS             complement(10872..11303)
                     /locus_tag="PKI23_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNN36595.1"
                     /translation="MSQRRRFTARSSMTLSTLCALTLLGAASMANAEAKQPPADYTQH
                     CAACHGESGKGDGIMAEGMKKTPADLTQIAKKNGGTFPTERIKQVVSGTLDDRTIARV
                     HGPKEMPVWGRYFYEDSSSSHAIAEARVGTLVEYLKTIQQK"
     gene            complement(11519..12013)
                     /locus_tag="PKI23_06365"
     CDS             complement(11519..12013)
                     /locus_tag="PKI23_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNN36596.1"
                     /translation="MLNREQTQQFAARLQQQCQLLDQLLDALEQERALIEHRDAEQLS
                     ALLPTKHQLLQQLDALHRDFQHWLSASGLPREPALIEQLIRSAPLLQESPALTIQWRT
                     FKHKSRRCQHANSVNGQLIKQGHERTRRLINLLLGREEQTAVYDQAGMTLQQENSRRS
                     LGSV"
     gene            complement(12016..12327)
                     /gene="flgM"
                     /locus_tag="PKI23_06370"
     CDS             complement(12016..12327)
                     /gene="flgM"
                     /locus_tag="PKI23_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNN36597.1"
                     /translation="MIDFKTQPPTPLPVPERPSGTAPAESRTDNRPAAEASGTAQSEA
                     VRISDQGRSLQRLEQQLPAGEVREGKVAEIRRAIDEGRYQPNPEQTARKMLSFERMLD
                     Q"
     gene            complement(12440..13075)
                     /gene="flgA"
                     /locus_tag="PKI23_06375"
     CDS             complement(12440..13075)
                     /gene="flgA"
                     /locus_tag="PKI23_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNN36598.1"
                     /translation="MAVDDLTAQAEKFLRQRIGSADSAETRFEITVGPLDRRMRLSAC
                     AVPLQFQAPTGELLGRVSIKASCNSSTQGWSLYIPANVRQFRPVVVAAHPISRGSALD
                     ASAITLRQSDISTLPQGYFTDPRQLLEQLARRDIAPGEPLRQSLLQPQRLIKRGDEVV
                     ILAEVDGLTVRSSGIALADGAAGAQIDIRNLRSERIIRARVHSAGTVMVRL"
     gene            13364..14314
                     /locus_tag="PKI23_06380"
     CDS             13364..14314
                     /locus_tag="PKI23_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003162737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheV"
                     /protein_id="HNN36599.1"
                     /translation="MAGVLDSVNRRTQLVGQNRLELLLFRLDGRQLYGINVFKVREVL
                     NCPPLSSLPRSNPLVRGVAHIRGSTIPIIDLSRAIGQRPAEELEQKFIIITEYNLKVQ
                     AFLVSAVERIVNMMWEEVHPPPSGAGNQNYLTAVTEVDRQLVEIIDVEKILSEVAPRS
                     EAITLTGIDDALIAQGGQAHVLVVDDSRIARKQVLNCLASVGIEATSCQNGKEALEFL
                     KRLADEGQEPARKFLMVISDIEMPEMDGYTLATEIRADARLKDLHVLLHTSLSGVFNK
                     AMVEKVGADNFIAKFQPDELTQAVIDRLKAVHPAVHSTAA"
     gene            14368..15186
                     /locus_tag="PKI23_06385"
     CDS             14368..15186
                     /locus_tag="PKI23_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNN36600.1"
                     /translation="MTIESGDYRLFRDFLERSCGIVLGDDKEYLVKSRVGTLMREQGI
                     GTLGELARRLESTQQRQLRKSVLDAMTTNETMWFRDGYPFDILRERLLPELARSKQRV
                     RIWSAACSSGQEPYSIAMVVEEYLSSQLAAFAGGIEIVATDLSSQVLQQAQSGIYAES
                     ALARGLSPERRKRFFDPVEGGYQVKPAVRGRISFRQINLLEDFTALGNFDLIFCRNVL
                     IYFRMELKREILRRLRGRLKPGGSLLLGASETTLDLGHCYQMEYCNPGVIHRAC"
     gene            15367..15771
                     /gene="flgB"
                     /locus_tag="PKI23_06390"
     CDS             15367..15771
                     /gene="flgB"
                     /locus_tag="PKI23_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008029381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNN36601.1"
                     /translation="MAINFDRALGLHPQALQLAARRAELLANNIANSDTPGYQAQDID
                     FSRLLAQQDDGRRSTSGLLRTRDTHLSVGGAEGFDQQVAYRQPLQPSIDGNSVDLQTE
                     QAEFAKNLLHYQTSLTLLRGRFDGLSRAIKGE"
     gene            15773..16207
                     /gene="flgC"
                     /locus_tag="PKI23_06395"
     CDS             15773..16207
                     /gene="flgC"
                     /locus_tag="PKI23_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNN36602.1"
                     /translation="MSLGQIFDIAGSAMSAQTVRLNTTSSNLANADSASSSIDRTYRA
                     RHPVFSAVQQQVMEQGLFGEAAAAQAGVEVAGIVESQAPLQPRYQPEHPMADQDGYVY
                     YPNVNPIEEMADMISASRSYQVNVEMMNTAKEMIVQTLKLGG"
     gene            16258..>16718
                     /locus_tag="PKI23_06400"
     CDS             16258..>16718
                     /locus_tag="PKI23_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNN36603.1"
                     /translation="MSNVTGVQSATDLYAEISKRPDVKKAGDDKNQFLELMVAQLKNQ
                     SPLDPQNGGEFLAQLAQFSTVEGINTLNETVAGYGGSMRSSQALQATALVGRTVQVPS
                     KVGELGGGKPLSGEIELPNNTDSLLLNISTESGALVRQFALGAQKAGSLPF"
BASE COUNT         2800 a         5080 c         5734 g         3104 t
ORIGIN      
        1 cagtaccagc agcaggtgct ggacagcgtc gaggcctact ttcgcgcctg ccacgagctg
       61 ccgtcgccgt cggtggcctt caccgcgacc actgaacgtc tctgggggcg tggcagtccg
      121 tacaaccccc tctcggggtt cccgcctgac atgccgtact tctgcctgcg cgtgccgacc
      181 ggcggtggca agacctggct ggcggcgaag agcgttcagc ttgccaacac gcatctgctg
      241 cgcaccgaac acagcgtgat cctgtggctg gtgccgagca agccgatccg cgaacagacc
      301 ctgcgcgcgc tgcgcgaccg gctgcatccg tatcacgcgg cacttcgtga agccggcccg
      361 atcacggtga tggacctcga cgaggccaag agtgtgaccc gcgcgacgct ggacacctcc
      421 accaccgtca tcgtcgccac ccgccaggcg tttcaggtgg aggacgagga gtgccgcaag
      481 gtgtatcaga gcagcggcgc gctgatgcac cacttcgaca acctgtcgcc cacccagcgc
      541 gacggcttgt tgagcgaggg cgcaggtgca gagcgcacga cgccctgttc gttggccaac
      601 gtgctgcggc tgcgccggcc gttcgtggtg gtggatgagg cgcacaacaa ccgcaccgaa
      661 ctcgccttcg acatgctggc gcgctttcgc cccagcggcg tgctggaact caccgccacg
      721 cccgatctgg aacgcacgcc cagcaacgtg ctgcacagcg tctgcgccgc cgagctgaag
      781 gcggaggaga tgatcaagct gccggtggtg ctggagaccg agccgaactg gcagcagtgt
      841 ctggccgacg cgatcggccg ccgcgaggcg ctgcacaggc tggccgatga ggcacgccgc
      901 gccggcgccg attacctgcg gcccgtcgtg ctgattcaat ccgagccgcg ccgtgccggc
      961 gtggaaacgc tggatttcga gcgggtgcgc caggagctga tcaccaatca cggcattccg
     1021 gcccgcgaga tcgtcgtcgc caccggcgag gaacgcggcc tggagcagat cgacaccaac
     1081 tacaagctgg gcattgccga tccagactgt ccggtcaagt tcgtcatcac ccagaaggcg
     1141 ctggccgaag gctgggactg cccgttcgcc tacatcctgg tgagcatggc ttcattgtcg
     1201 tcggcgacgg ccgtcgagca gttgctgggc cgggtgctgc gccagccgaa cgcacgccac
     1261 ttcgacgatc cgctgctgaa ccgctcctac gccttcgtgg tgtcgaggaa cttcgccgaa
     1321 acggcgggcg cgctgcgcga tcggctggtg gccggcgccg gcttcgagcg gcgcgaggtg
     1381 gcgcagttcg tcaccgccgc ccgggccgag caggcccgga tcgatctcga cggccacgcc
     1441 ggccgcgcgg tgatgcgccc ggtggtggta gtgctgcccg agaagcccga cctcaagcgc
     1501 ctgccccagc cggtgcgcga caagctgagc tgggacggga acctcaatac cttgaccatc
     1561 agcacgccgc tgaccgagga cgagaccgac acgctcacgg cggcggtgac ctcagaggcc
     1621 gcggccacgg cgattgccga agccgccgaa atcagccgca ccacggcgat cgagttcttc
     1681 cagacgccgg ccgaacgcgg cgagcgcttt gcggtgccgc agcttgcgtt gcgtgtgcag
     1741 ggcgcgctgc aactgttcga cgacccggag ctgctcgact acccctgggc gctgtcgacc
     1801 tgcgatgccg cgccgaccac cgcagagctg gccatgctcg gcgcgtcgat gaaggtctcc
     1861 gaaggcggcg agatcgatgt cgacgacggc ggccggctgg tgtcgcgctt tctgcccgag
     1921 ctgcaacgcg atctcgggct ggtctgcccg cccgagcagt gggatgaact gcggctggcc
     1981 acctggctgt gccggaacct gccggagccg tcgctcaccc atgccagcaa gcaggccttc
     2041 gtggccgggt ggctgaccga actgctgcgc cgcgacgatt tcccgctggc gcgcgccagc
     2101 ctgcagaaat tcctgattcg caacctgctg gaggcgcgca ttcgacagtt gcgccggcag
     2161 gcagtgggcc gggccttcca gcagacgctg ttcggtgacg atgttgggca gcgggtggcg
     2221 gtgagcgacg tttacgcctt cgagttcgcg ccccacgcct atgcgccggc ccgcgactac
     2281 gatggccgct tcggccactt cgactttcgc cggcactact acggccgcat cggcgacttc
     2341 gacagcagag aggagttcga ctgcgcctgc tggctggaca tgcaggcgca gcgggggcgc
     2401 atcgcgttct gggtgcgcaa tctggtgcga cgcgagggga gttcgttctt cctgcagaag
     2461 gccgatggtc gtttctatcc ggattttctc tgtcagttgc cgggtcgcgc tggcgagtct
     2521 ggcccgatcc tggccgtcga gtacaagggt gccgaccgct ggagcgccgc cgacgatgac
     2581 cggctgatcg gcggcctgtg ggcgagtctc tccgacggtc gctgccgctt cgtgatggtg
     2641 accgacagag gctgggagcg gatcgaggcg cagtggtgat gtgctggagc gggtggcatg
     2701 ggtgagccgc tctggcatct gctgggtgtc gatgaggcgg cgcagcgcct cgacagcgat
     2761 cggctgaccg ggctgacggc ggacgaggcg ctgcggcgac ggcaactgca tggtccgaac
     2821 gagctccgcg aggcgaagcg gcgtgggccg ttgcgaatct ttctcgacca attcgccgac
     2881 ttcatgatcc tggtgctgct ggcggcggcc ctggtggccg gcctgatcgg cgactggcat
     2941 gacagcctgg tgatcgtcgt catcgtgttg ctgaatgccc tgctggggtt cgttcaggag
     3001 taccgggccg agcgggcgat ggccgcattg cggcggctgg cggcgccaca ggcgcgggtg
     3061 cagcgcgcag gagggctgct gagcgtgccg gccagcgaac tggtgccggg tgatcgggtg
     3121 ctgctcgaag cgggcaatgt ggtgccggcc gatctgcgcc tcatcgaggt gaaccagttg
     3181 cgcaccgacg aggccgcgct gaccggggag tcgcagccgg tcgacaagca ggcggcagcg
     3241 ctgagcgagg ccgagttgcc gctcggtgat cgccacaaca tggcctaccg gggcacccag
     3301 gtggtgcatg gacgggcggc ggggctggtg gtggcgaccg gcatggccac cgaactgggc
     3361 cgaatcgcca tgctgttgca gcagcagctt gccctcaaga cccccttgca gcagcggctg
     3421 atccggttcg gtcagcggct ggcgctggcc tgcatcctcc tctgcgtgct gatctttgcg
     3481 atgggggtgt ggcgcggtga gccgccgctg ctgatgtttc tgaccgcggt cagcctggcg
     3541 gtggcggcca ttcccgaggc gctgccggcg gtgatcacca tcgcgctggc gctcggcgcg
     3601 cgcagcatgg tcgggcagcg cgtgctgatc cgccggctgc ccgccgtcga aaccctgggt
     3661 tcggtcagct acatctgttc cgacaagacc ggcaccctga cccagaaccg gatgacgctg
     3721 gaatctctct ggctggcagg ttcatggtat ggccgcgatg cgctgaccac cgcgccggcg
     3781 ccgctgcgcg acgccctgct gcgggccgtg gcactcaaca acgatgcccg tcgcgatgca
     3841 gccggcggct gggttggcga ccccaccgag gtggcactcc atgaggcggc cgagactttc
     3901 ggccatggcg cagcggcgct gaccgtctcc gcaccgcgta tcggcgagat tcccttcgat
     3961 gccgagcgca agcggatgac cacactgcac cagcaaggag cggcgctgct ggcctgcacc
     4021 aagggggcgc ccgaaagcgt gctggcctgt tgcattggcc tgcagagcga agcgggcagg
     4081 gtcgcactcg atcagcaggc ggtgctggcg gcggccgaag cgatggcggc gcaggggctg
     4141 cgggtgctgg cggtggcctg tcgtgactgg gacgcgctgc cggagcggct cgacagcgaa
     4201 acggtggagc agtcgctgct tctgctgggg ctggtcggtc tgatcgatcc accgcggccc
     4261 gaggcccgtg aggcggtgca gtggtgccat cgcgccggca tcacgccggt gatgatcacc
     4321 ggcgaccatc cggccaccgc gctggcgatc gcgcggcggc tcggcatcgc cggtgcgacc
     4381 gaccgggcag tgaccggacc gatgctggcc acactcgatc cggagcagct tgggcaactg
     4441 gtcaccgagg tgcgggtcta tgcgcgggtg gcgccggagc agaagatcga catcgtccgg
     4501 gcgctgcagg cgcgcggcgc cttcgtcgcg atgaccggcg atggtgtcaa tgatgcgccg
     4561 gcgctgaagc gggccgacat cggggtggcg atggggcagg gtggcaccga ggtggcctgc
     4621 gaggcggccg acatggtgct gctcgatgac aactttgctt ccatcgtggc ggccgtgcgt
     4681 gaggggcggc gcatccacga caacatccgc cgcttcgtca agtatgcggt gaccaccaac
     4741 tcggcggaga tctgggtgct gtttctggcg cagtgtttcg cactgccggt gccgctgctg
     4801 ccgatccaca tcctctggat caacctgctg accgatgggc tgcccggcat cgccctcacc
     4861 cgggagccgg cggagcgtga catcatgcgg cgtccgccgc gtccgccgaa cgagagcctc
     4921 tttgcccatg gcctgtggca acacatgctc tgggccggat tgctgatggc gctgctgacc
     4981 ctggcgctgc aatcctgggc gctgcacaac gacatcgccc actggcagag catggtgttc
     5041 acgctgctga cgctgacgca gatggcccat gtgctggcaa ttcgctccga gcgcgagtcg
     5101 ctgttcaccc agggtcttgc cagcaacctg ccgttgctgg gtgcggtgct gctgacgctg
     5161 gccctgcaac tggcggtgct ctacctgccg ccgctgcaat cgatcttcag gactgcgccg
     5221 ctggcgctcg atgagctgct gctctgtgtc gggctggcgg cgctgctctg ggtggcggtg
     5281 gagatcgaga agtggctgcg ccgccgtggc gcgatgcagc ggacggcaac gggcggctga
     5341 tcggtcgctg tcagcggctc aggcggagtc agacgggcgc ggatagatgg tgtggtagac
     5401 caggccgacc agcacgctgc cgccgatgat gttgccggcc agcaccggca gcaggttgtg
     5461 gaagaagccg ccccaggtga tgacggtctg ggtcgccgtg ccgccgccga agtgctggat
     5521 cagcatcgcc agcggaatga agtacatgtt ggcgatgctg tgctcgaaac cggcggcgac
     5581 gaaggccgag atcggaaaca ggatcgccac cgccttgtcg acgatggtgc ggccggccat
     5641 cgccatccac accgccatgc agaccagcac gttgcagagc atccccctga agaaggcggt
     5701 tgccggcggc agcagcgttt tggcggcggc gatcttcacc gtctgctcgg cgatggcgcc
     5761 ctggttcatc tccgggtggc gtgacagaaa gaccagcagc gcaagggcag cggcgccgac
     5821 gaagttggcc agacagacca ccacccagtt gcgcagcagt tcatgggcac tgatgcggcc
     5881 atcggcccag gccagcacca gcaggttgtt gccggtgaac agctcggctc cggccaccgt
     5941 caccagcagc agccccagcg agaaggtgac gccacccagc agttgccggg cggcaaagcc
     6001 gagcgaggca tcggcggcca ccagcgtgta gtagagcgcg ccgaggccga tgaaggcccc
     6061 ggccagcatg ccgagcatcc acatcgaccg cagcggcagc cgggccttgg cgacgccgac
     6121 ctgttcgatg cgctgggcaa tctcccgcgg cgagaaggcg tcgaagccga agatctcgga
     6181 catggccggg ctgagcgatc agggcatcag cagccgtcgc agatggcggc ggtgatgcag
     6241 cgtgtcgccg aagctgaact tgttccactg ggcacggcgg aagtggagtc cggcgttgca
     6301 ttcgtgggtg aagcccatgc caccatggaa ctggatggcc cggtcggagg cgaagtagaa
     6361 cgactcgttg gagtggacct tggccatccg caccgccacc tccgactcgc cgccgtcata
     6421 gccgttgaag agcgaagcgg cgtagtagag gtgggagcgg ctgtgctcga ccgcgatcat
     6481 cgcatcgacg gtcgggtgct tgagcgcctg atagctgccg atcagctggc cgaactgttt
     6541 gcgggtcttc aggtagtcga ccgtcagcag cagtgcgctg ttggcgccac cggcactctc
     6601 ggcggtgtgc agcagcgcac ccagtgcatg acaccacttc agtgcggcca gcgcatcgcc
     6661 gccggtcagt tgtgcatcgc ttcccacttc gaggccatcg agctgcaacc ggtggctgcg
     6721 gcgggtctcg tcgatcacgg tttcacgccg caggcggcct tgcagttgtg ggcccgagag
     6781 acagaacagc gccggggcgc cgtcgagctg acaggcgacg atgaccaggt cggccacggc
     6841 tgcatcgagc acgaaggctt tgctgccgct gagcacgaaa ccattgccgc tgcgctgggc
     6901 ggtggcgcgc aactggtcgg gctcccatgc gccgtggggc tcgaacaggg ccaccgaacc
     6961 gatggccgcg ccgctggcga gtttcggcag cagatggctc ttttgcgcct cgctgccggc
     7021 cttgagcagc agctgcccgg ccagcgtggt ggcgatgaag ggggtgccga ccaggtgtcg
     7081 gcccatcggt tcgaccaccg ataccagctc tttggcactg agaccggcgc cgccgaactc
     7141 ttcgggaatc atcatgccga gccagcccag ctcgccgatg ctgcgccagc catcggtggt
     7201 gtaaccgcgc tcgtcgtcga gcagttggcg cacggtggcc atcgggtact gttcgcggaa
     7261 aaagctgact gcggagtcgg agatcatccc ctgctcttcg gtgaactcga tcgcgccttc
     7321 catcttgatc attgtctgtt gctcctgaag ctgttccgtg tcacttcggc aggtcgagca
     7381 cccgctcgcc gagaatgttg tgttgcactt cgctggtgcc gccgccgatg gtggccgagt
     7441 aggcgttcat cgcggcacgc tgccattcgc caccctgcac cgcctcgggg tcgcccatgt
     7501 agaggttggc acggccgccc tgcagctcga cggcgaagta ggcgagccgg cgcatgaact
     7561 ccgatccgaa cagcttgccc atcatcatgg tcgcggccgg gcgctccggc gtcagcagct
     7621 tttcgatcct ggcgcgctgg ctggtcagcg cgatgctgcg ctgatcgatc agaaagttga
     7681 gcagcttgtc ctggaccagc ggatcctcca gtgccgggcg gccgtcacgc tcgatcagcc
     7741 ggctcatctc gatcacatcc tgggtggaga agacggtgga catgttgccg ccggcctgac
     7801 cgccggccgc gccacgttcg aaggccagca ccgccatcgc caccagccag cccttgcctt
     7861 cttcgccgac cagggtcgat tcatggatgc gggcattggt gaagacggtc tgattgaagc
     7921 cgtactcgcc ggtgatcttt ttgatcggga tcacctcgat gccgggcgtc tgcatcgggc
     7981 agagaaagaa gctgaggccg gcatacttgc tgctggtgtt gggaatcgag cgcgccagca
     8041 gaatcatgtg cctggcgtag accccgaggc tggtccagat cttggcgcca ttgacgacga
     8101 agtggtcgcc atcacgttcg gcacggcact gggcgttgcc gaggtcggaa ccatggtccg
     8161 gttccgagaa accctgacac cagatctcct cggcgttgag aatgtttttg atgaactgct
     8221 ttttatgggc ctcgctgccc aggtgcagaa tggtcgggcc ggcccacgac aggccgatgg
     8281 cgttgaccat gaacgggacg ttggcgcggg tcatctcctg agtggcgatg tcctgcaaaa
     8341 tctgtggctg accgccgccg ccgtattcct ttggccaggc catgcccagg tagccggcct
     8401 cgtacacctt gcgctgccag tcgcgcaggt agtcgaactg ctgctgagtg tcgacctcca
     8461 tgaaggattg agggaggatg aaatcgggtt tggccgggcg gttgcgctcc agccagccgg
     8521 ccacctcgcg gcggaactcc tgatgtgctt tgggttcggg tgtggtcatt gatcgttcct
     8581 cgatcgttgt ttgagaggtg ttggggggtc tcccggcgtc agccggggtc ggtcaggtgc
     8641 cttgcagaaa atcgagaatc agccggttga cctgttcggg ctgctcctga tgcaggaagt
     8701 ggccgccatc ggcgatgaca cggttggtca ggccggcggg gaacagggcg tcgatgttct
     8761 ggctgaactc ggcaccgatg cagccatcca gcgcgccatg gatgttcagc gtcggcacct
     8821 ggatcggttc acggccaatg cgcgcctgca gcggtgccag cagcgggtcg tgccgttcgg
     8881 gattcatggt gcagcggtag tagccgagcg ccgctgccag cacgccgggc tgggccaggg
     8941 tctgtttcag tgcgcgcatc tcttcggccg gaaaatccca gccgggtgac cagtcgcgcc
     9001 acagccgttc gatgaaggcg aaatcttcca gtggcacggc caggtcggcc agggcggtct
     9061 gaaagaagaa gatataccag gagcgcttct gctgtgccca gttgttcagt agcgcggtgc
     9121 ccataccgcc gtagggcagg gcgagggtga tcagcttgct gaccttctcg ggcgcgaaca
     9181 gcgctgccgc gctggcggcg gtggagcccc agtcgtggcc gatgacgact gcctgctgcg
     9241 caccgagcgc ctcgatcagc gccagcacat cctgcccgag tgccgcggtc tggtagttgc
     9301 cgttggcagc cgctgcggtg ggcgcatagc cgcgcatgaa gggggcgacg gcacggtaac
     9361 cggcctgcgc cagtgccggc aactggtggc ggaacgagcg tgcggtgtcg gggaagccgt
     9421 gcatgcacag caccagtggc ccttcgccct gctccagaaa ggtgaactcc aggccgttcg
     9481 cagtgacctt gccctgtttg aatggttgca tgagcgcctc ttgatcgttt ttcagtgtca
     9541 gtgtctggcg aaggctgcgt atcagctcga aatcagggag agcagttcgc tgcgggtgct
     9601 ggggttgctc tggaactgcc cgagcatcac cgaggtcttc atccgcgaat tctgcttctc
     9661 gacgccgcgc atcatcatgc acatgtgttg cgcctcgatg atcacggcca ccccctgggc
     9721 ctgggtgacc ttctgaatgg tgtcggcgat ctgccgggtc aggttctcct gcacctgcaa
     9781 ccgccgggcg tacatgtcga cgatgcgggc aaacttcgac aggcccagaa tcttgccgct
     9841 ggggatgtag gcgacatggc acttgccgat gaacggcagc aggtgatgtt cgcagagcga
     9901 gtagagctcg atgtcctgca ccagcaccat ctcgttgctt tccgattcaa agatggcgtt
     9961 gttgatgatc tggtcgatgt cctggtggta gccgcggttg aggaaggcaa aggcgcgggc
    10021 ggcgcgctcg ggggtgtctc tcagtccgtc acggttgaga tcctcgccga tgctctcgag
    10081 cagcttctga taggcctctt tcattgtttt gctccggagt tcgctgtgtg gggcagcggg
    10141 atgttacgtg atcgaccacg gctgaaaaac cctgggctca caaggttggc ggctgctggc
    10201 gcagccgcag atagagtgcg ccggtgccgc catcacgcgg cagtgccgag cagaaggcca
    10261 gcagttgcgg aaaccttggc agctctgcga ccaaccacga cttcagcacc gcagtgccgc
    10321 tggcggagcc atggcccttg ccatggatga tcagcagtgt ccgcaggccc tcttgccagc
    10381 tctggtcgat gaacgcgcgc aactcgattt cggcctgccg ttgccgcaga ccgtgcaggt
    10441 cgagcacggc ctcggtgcgg aatttgcccc gtttcagttg cagcagttgc cgcggctgga
    10501 tgccgggacg ctgaaacagc accggttctt cgccgctgca gggtcggggc gccgcttcag
    10561 cgggctgtct ggcgtcatcg tcggatggtg gagatggaga gcgacgtggc ctgaattggc
    10621 cggttttgct ggggatgggg gcgcagcccg cagcgggagt gcgaatcaca tcagccgtgg
    10681 cctgctggaa cagtgcgcgc tcctcggtgg caagcgtttc gcccggagca ggtggtgtgc
    10741 ggttcttgcg catggcagac agaagatgaa ggtcaaacgg cacgacccag tctctgctcc
    10801 agtcggcagc caagccgaca atcgcggacg aagcgcgcgg ggtacgcttc gtccacggcc
    10861 cgagggagag cttacttctg ctggatcgtc ttcagatatt cgaccaaggt gccgactctg
    10921 gcttcggcaa tggcatggct cgacgaggag tcctcataga agtagcgacc ccagaccggc
    10981 atctccttgg ggccatgcac ccgggcgatg gtgcggtcat ccagggtgcc gctcacgacc
    11041 tgcttgatcc gctcggtcgg gaaggtgccg ccattcttct tggcgatctg cgtcagatcg
    11101 gcgggggttt tcttcatgcc ctcggccatg atgccatcgc ccttgccgct ctcgccgtgg
    11161 caggcggcgc agtgttgcgt gtagtcggcc ggtggttgct tggcttctgc gttcgccatc
    11221 gacgcagcgc cgagcagggt caacgcgcag agcgtggaga gggtcatcga agagcgagcg
    11281 gtgaagcgtc gacgttggga catttttcat tctcctgggt tgggggcttc atcttattgt
    11341 tttagcggca cgcacagggt atccgcactt caagaatcga cagccggaac gatacccgat
    11401 tgtgaacgtc gatgccagat tttgtatggc tatggcgcct ttggcttgcc gactgagtac
    11461 agaatgggtg tgatcgcgtg gatcgagttg ccgaaggggg atggatatgc ggcgtttctc
    11521 agacgctgcc gagagagcgt ctgctgtttt cctgctgcag ggtcattcca gcctggtcat
    11581 agacagcggt ctgctcttcc cggccaagga gcaggttgat gagccgccgg gtgcgttcat
    11641 gcccctgttt gatcagttga ccgttgacgc tgttggcatg ttggcagcgt ctgcttttat
    11701 gtttgaaggt gcgccactgg atggtcaggg ccgggctctc ctgcagcagc ggagccgatc
    11761 tgatgagctg ctcgatcagg gctggttcgc gcggcagacc ggacgcgctg agccagtgct
    11821 ggaagtcgcg atgcagggca tcgagctgtt gcaacagctg atgcttggtc ggcagcaggg
    11881 ctgagagctg ctctgcgtca cggtgctcga tcagcgcacg ctcctgttca agggcgtcga
    11941 gcaactggtc gagcagttgg cactgctgct gcagccgtgc ggcgaactgc tgtgtctgtt
    12001 ccctattcag catggttatt gatcgagcat ccgttcgaag ctgagcatct ttctggcggt
    12061 ctgctcagga ttcggttggt aacggccttc atcgatggct cgacgaatct ccgcgacctt
    12121 gccttcgcgc acttcgcctg ccgggagctg ctgttccaga cgctgcagcg agcggccctg
    12181 atcgctgatc cgcaccgctt cgctctgggc cgtgccggat gcctctgcgg cgggcctgtt
    12241 gtcagtgcgc gactcggcgg gggcggtgcc actcgggcgc tccgggacgg gcagcggtgt
    12301 gggcggctga gttttgaagt caatcaccat ggtgcactcc tgtcagaatc gggtgggcaa
    12361 gcggcgcttg cctgcatggg ctgtatcggc ggctttgcgc cactctttag gcggcaatgt
    12421 actttttcgg atggcgtttt cacagtctga ccatgaccgt gccggccgaa tgcacgcgcg
    12481 cccggatgat tcgctcgctg cgcaggtttc tgatgtcgat ctgcgcgccg gctgcgccat
    12541 cggcgagcgc gatgccgctg ctgcgcacgg tcaggccgtc cacttcagcc aggatcacca
    12601 cctcatctcc ccgtttgatc agccgttgtg gctgcagcag gctctggcgc agcggctctc
    12661 cgggggcaat gtcgcgacgt gccagctgtt ccagtaattg ccgcgggtcg gtgaagtagc
    12721 cctgtggcag tgtcgagatg tcgctctgcc gcagggtgat ggcgctggca tcgagtgcgc
    12781 tgccacgcga aatcgggtgg gcagcgacca ccaccgggcg gaactgacgc acgttggcgg
    12841 ggatgtagag cgaccagccc tgggtgctgc tgttgcaact ggccttgatc gagactctgc
    12901 caagcagctc gccggtcggc gcctgaaatt gcaacgggac ggcacatgcc gacaggcgca
    12961 ttctgcggtc gagtggcccg accgtgatct cgaagcgtgt ttcggcgctg tcggcgctgc
    13021 cgattcgctg acgcaggaat ttctcggcct gggccgtcag gtcgtcgacc gccatgctcc
    13081 agcatttgat gctgagcagg ctgcagaaaa gagccagtgt caggccgagg agccggtggt
    13141 ttccttccct tttcatggtc atgaactacg ctttgaatca atggcgatgc agcgctttgc
    13201 agtgacgaaa atcaccctcc agcagcgggt tgggtgtcgg aattccgtca ttttttgccg
    13261 atgcccctgc atgaaccggg taatgacgat agataaatgc agacttcgtg ccgatgtttg
    13321 atcgaaaagg ttgaacgcgg ccacaaccgc agcggagggg aacatggctg gagtgttgga
    13381 cagcgtcaac aggcgcacgc agctggtggg ccagaatcgt ctggagctgc tgctgttcag
    13441 actggacggc aggcagctct atggcatcaa tgtgttcaag gtcagggagg tgctgaactg
    13501 cccgccgctg agctcgctgc ccaggagcaa tcccttggtg cgcggcgtgg cgcacattcg
    13561 tggcagcacc attccgatca tcgatctgag ccgggcgatt ggccagcggc cggcggaaga
    13621 gctggagcag aagttcatca tcatcaccga gtacaacctg aaggtgcagg cctttctggt
    13681 cagtgcggtg gagcgcatcg tcaacatgat gtgggaggag gtgcatccgc ctcccagcgg
    13741 cgcgggcaac cagaactatc tgaccgcggt gaccgaggtc gatcggcaac tggtcgagat
    13801 catcgacgtg gagaagattc tgtcggaggt cgcgccacgc tcggaagcga tcacgctgac
    13861 cggcatcgat gatgcgctga tcgcccaggg tggtcaggcc catgtgctgg tggtcgatga
    13921 ttcgaggatc gcgcgcaagc aggtgctcaa ctgcctcgcc agtgtcggca tcgaggcgac
    13981 ctcgtgccag aacggcaagg aggcgctcga gtttctgaaa cggctggcgg atgaaggtca
    14041 ggagccggcg cggaagttcc tgatggtgat ctcggacatc gagatgccgg agatggatgg
    14101 ctacaccctc gccaccgaga ttcgcgcgga tgcccgattg aaggatctgc atgtgctgct
    14161 gcacacctcg ctgagcggtg tcttcaacaa ggcgatggtt gaaaaagtgg gcgcagacaa
    14221 cttcattgcc aagttccagc ccgatgagct gacccaggcg gtgatcgatc gcctgaaggc
    14281 ggtccatcct gccgtgcact ccactgcggc ctgagcgagt tgcaaggcat ggaggttggt
    14341 cagtggcgag agatggcgag gtgagccttg acgattgaaa gtggcgatta ccggcttttt
    14401 cgcgatttcc tcgaacgcag ctgcggcatc gtgctgggtg acgacaagga gtacctggtc
    14461 aagagtcgcg ttggcacgct gatgcgcgag cagggcatcg ggacgctcgg cgagctggcc
    14521 aggcgtcttg agtcgaccca gcagcggcag ttgcgcaaat cggtgctgga tgcgatgacg
    14581 accaatgaaa ccatgtggtt tcgcgatggc tatccgttcg atatcttgcg tgaacgtctg
    14641 ctgccggagc tggcccggtc gaagcagcgg gtcagaatct ggtcggcggc ctgctcctca
    14701 ggtcaggagc cctactcgat cgcgatggtg gtcgaggagt atctctcatc gcagctcgct
    14761 gcctttgccg gtggcatcga aatcgtcgca accgatctct cctcgcaggt gctgcagcag
    14821 gcacagtccg gcatctatgc cgagagcgcc ctggcgcgcg gtctgtcgcc ggagcgcaga
    14881 aagcgctttt tcgatccggt cgagggcggg tatcaggtca agccggcagt gcgcgggcgg
    14941 atcagtttcc ggcagatcaa cctgctggag gatttcaccg ccctgggcaa ctttgatctg
    15001 atcttctgcc gcaatgtgtt gatctacttc cgaatggagt tgaagcggga gattctgcgg
    15061 cggctgcgcg gacggctcaa gccgggcggc tcgctgctgc tgggtgcttc tgaaaccacc
    15121 cttgatctgg ggcactgcta ccagatggag tactgcaatc ctggcgtgat ccatcgcgcc
    15181 tgctgaactc tccttgccgg gtcggccggc aagaaggtgc cgtgccgggc atccggcagg
    15241 ctggcaagag tttgccgttc acgcctgcat tccacgaatt ctctttacaa ttctttgtaa
    15301 aacaataaca aaatattttg gcattgactt tgcattctga tacctgacgt catcaggagc
    15361 agagtcatgg ccatcaactt cgaccgcgcg ctcgggcttc atccgcaggc cctgcaactg
    15421 gccgctcgcc gtgccgagtt gctggccaac aacatcgcca acagcgacac cccgggctac
    15481 caggcgcagg acatcgactt cagccgtctg ctggcgcaac aggatgatgg gaggaggtcg
    15541 accagcgggc tgctgcgtac ccgtgacacc cacctgtcgg tgggtggcgc cgaaggcttc
    15601 gaccagcagg tggcctatcg ccagccgctg caaccctcga tcgatggcaa cagtgtcgat
    15661 ctgcagaccg agcaggccga attcgccaag aatctgctgc actatcaaac cagtctgacg
    15721 ctgctgcgcg gacgtttcga tggcctcagc cgcgccatca aaggagagtg agatgtcact
    15781 gggtcagatc ttcgacatcg ccggttcggc gatgagcgca cagacggtgc gcctcaatac
    15841 cacctccagc aacctggcca acgccgattc ggccagcagc agcatcgacc gcacctaccg
    15901 tgcgcgccat ccggtttttt cggcggtgca gcagcaggtg atggagcagg gtctgttcgg
    15961 cgaagcagcg gcggcgcagg ccggtgtcga ggtggcgggc atcgtcgaga gtcaggcgcc
    16021 gttgcagccg cgctatcagc cggaacatcc gatggccgat caggatggct atgtctacta
    16081 ccccaacgtc aatccgatcg aagagatggc cgacatgatt tcggcctccc gctcctatca
    16141 ggtcaatgtc gaaatgatga acacggccaa ggagatgatc gtgcagaccc tgaagctggg
    16201 cgggtaaagc gccatggtgc gtgacgggga acagggcaaa ccagttggag caggatgatg
    16261 agcaatgtca cgggtgtgca gtcggccacc gatctgtatg ccgaaatcag caaaaggccc
    16321 gatgtcaaaa aggccggtga tgacaagaac cagtttctgg agctgatggt cgcccagttg
    16381 aagaaccaga gcccgctcga cccgcagaac ggtggtgagt ttctggcgca actggcgcag
    16441 ttcagcaccg tcgagggcat caacacgctc aatgagacgg tcgcaggcta tggcggcagc
    16501 atgcgctcgt cgcaggcgct gcaggccacg gcgctggtgg ggcgcacggt gcaggtgccg
    16561 agcaaggtgg gtgagctggg cggcggcaag ccgttgagtg gcgagatcga gttgccgaac
    16621 aacaccgaca gcctgctgct caacatcagc accgagtcgg gggccctggt caggcagttt
    16681 gcgctgggtg cgcagaaggc cgggagtctg ccgttcag
//
LOCUS       DAORCQ010000045        16681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2161_length_16681_cov_6.445808, whole genome shotgun sequence.
ACCESSION   DAORCQ010000045 DAORCQ010000000
VERSION     DAORCQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16681
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2161_length_16681_cov_6.445808"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1210)
                     /gene="hisC"
                     /locus_tag="PKI23_06405"
     CDS             complement(104..1210)
                     /gene="hisC"
                     /locus_tag="PKI23_06405"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNN36604.1"
                     /translation="MSCDFYQLANPGVRDLTPYEPGKPIDELERELGITGSIKLASNE
                     NPLGASPQASEAARAALSELSRYPDGAGFRLKAALARKFALDQRRLVLGAGSSDLLRL
                     AIEAFVCAGDQVVISQYAFAMYAIYAQAAQAERIVVPARDWGHDLDAMAAAVTDRTRM
                     LFIANPNNPTGTWVGQADLLRLLKRLPQQVLVVLDEAYAEYIDQPDYPNGMALGLEFP
                     NLIVMRTFSKAYGLAALRVGYAIADPATADLMNRIREPFNVNSIALAAAEAALADEAH
                     LQRSKAVNQQGLVQLREAAERLGLRAIPSVGNFIAIDMGRVALPIYEALLRKGLIVRP
                     LAGYGMPNHLRVTASTEAENSRFIKALQEVLEAG"
     gene            complement(1232..2347)
                     /gene="pheA"
                     /locus_tag="PKI23_06410"
     CDS             complement(1232..2347)
                     /gene="pheA"
                     /locus_tag="PKI23_06410"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNN36605.1"
                     /translation="MSQRQDEEARQLAAVRQQIDSIDSELLRLINARAQCAQQVAAIK
                     IAHAEGVPSHFHRPEREAQILAQVQQNNPGPLPNAAVALLFRELMSACLALEKPLRVA
                     YLGPAGTFSEAAAVKHFGQAIEGMAQPTIADIFAQVERGLADYGLVPVENSTEGMVGQ
                     TLDLFVDTALTLCGEVELAVQHCLLGRQAHGTPRRVLSHQQSLGQCRNWLNAHLPGVE
                     QQAVSSNSEAARLASEDPQVVAIAGKMAAEHYGLQVLAERIEDFSSNTTRFLVLGQQP
                     PGISGHDKTSLLVSVPNRPGALYRLLAPFEHRGISLTRLESRPAKSHPWRYRFFIDFE
                     GHRLEPDIAALLEEIEQHSAEVKWLGSYPRASNGSDL"
     gene            complement(2354..3436)
                     /gene="serC"
                     /locus_tag="PKI23_06415"
     CDS             complement(2354..3436)
                     /gene="serC"
                     /locus_tag="PKI23_06415"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNN36606.1"
                     /translation="MARVYNFCAGPAALPEPVLRKAQAEMLDYQGRGLSVMEMSHRSD
                     EFIAIAAQAEANLRRLLDIPAHYRVLFMQGGATAQFSAVPLNLLGATGHADYINTGSW
                     SQKAIEEGRRFGRVNEAASSEAEKFTTIPPLAEWRLDATAAYCHYTPNETIGGVEFSF
                     IPDTGAVPLVADMSSTILSRPLDVSRFGVIYAGAQKNIGPAGLTLVIIREDLLERATA
                     TIPMVLDYRLTAKNESMYNTPPTYAWYLAGLVFEWLLEQGGLTAIGEVNRRKAEKLYR
                     YLDGSGFYRNPIDPACRSWMNVPMILADAKLDGAFLKGADAAGLLNLKGHRSVGGMRA
                     SLYNAVPEAAVDALIDYMSDFARRNG"
     gene            complement(3494..6061)
                     /gene="gyrA"
                     /locus_tag="PKI23_06420"
     CDS             complement(3494..6061)
                     /gene="gyrA"
                     /locus_tag="PKI23_06420"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNN36607.1"
                     /translation="MGESAKEIVPVSIEDELKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLHAMNELGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQTFSLRYPL
                     VDGQGNFGSIDGDSPAAMRYTEIRMSRLAHELLADIDKETVDFVPNYDGTEQIPEVLP
                     TKVPNLLINGSSGIAVGMATNIPPHNLTEVVNGCLALIDDPDITIDALMESIPGPDFP
                     TAGVINGRAGIIEAYRTGRGRVRIRARARIENDSKNGREVIIVEEIPYQVNKARLIEK
                     IAELVREKKLEGISELRDESDKDGMRIVIELRRGEVGEVVLNNLYAQTQMETVFGINC
                     VALVDGRPGVLNLKEMLEAFVRHRRQVVTRRTLFLLRKARERGHILEGLSVALANIDA
                     VIELIRNSATPALAREALVAQPWQASEVVALLEQAGASACRPDGLPAEYGLDGESYRL
                     SPEQAQAILDLRLHRLTGLEHDKLRSEYQETLGNIANYEEILGSTARLIEVIRQELTE
                     IRNQYGDARRTEILLDQLDLTTEDLISEEERVVIFSHGGYAKSQPLDAYQAQKRGGRG
                     KSATGVKEDDFIETLLVASSHDTLLCFSTLGKIYWIKVYQIPVASRASRGRPIVNLLP
                     LSEHERITAVLPIREYPEDRYVFMATADGTVKKTPLVEFSRPRSLGIRAIDLDESDTL
                     VGAALTDGNGEVMLFSSDGKAIRFQEAQVRPMGRTARGVRGIRLNDAQRVIALIVPAA
                     DGELLMASLNGYGKRSAIAEFPLHGRGGQGVISMQLSERNGALVSVIQVCDKDHIMLI
                     SDQGTLVRTRCDETPLLGRNTQGVRLIKLAEGEHLVGVERIAEDEGDEESDDGEALQ"
     gene            6246..7610
                     /locus_tag="PKI23_06425"
     CDS             6246..7610
                     /locus_tag="PKI23_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNN36608.1"
                     /translation="MSALPADAPHLQQIDLLIEARWIIPIIPAGEVLEHQAIAIDQGA
                     IRDLLPSTEARQKYRPRQHLTLHEHVLLPGLVNAHGHAAMSLFRGLADDLPMMEWLTD
                     HIWPAEQHWVSETFVEDGTELAIAEMLRSGTSCFADMYFFPDRVARVASRSGIRAQVC
                     FPVLDFPTLWAQQADDYLHKGLMLHDDYRDHPRVRIAFGPHAPYSVSDAPLSRIATLA
                     EELDLSIQIHLHETAQEVEEAMARSGRRPLMRLGELGLLSPRLQAVHMTQLLDSEIEL
                     LARHGIHVIHCPRSNLKLASGMAPVARLLDAGVNVALGTDSAASNNSLNLFDELAMAA
                     LLGKGVSGDARAIDAHRALELATLGGARALGLGEQIGSLEAGKLADLIAVRLDDINQQ
                     PLYHPASQLVYSASGNRVSHLWVAGRPLLSDGQLQSIDLASLRERVNGWAARIGTDGI
                     KGRP"
     gene            7680..8732
                     /gene="purM"
                     /locus_tag="PKI23_06430"
     CDS             7680..8732
                     /gene="purM"
                     /locus_tag="PKI23_06430"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNN36609.1"
                     /translation="MHPKAPFSTHGLSYRDAGVDIDSGNALVERIRPLARSTARPELL
                     GGIGGFGALCRLPAGYREPVLVTGTDGVGTKLKLAQQADRLEGLGIDLVAMCVNDLIV
                     CGAEPLLFLDYYATGKLDLESATRIISGIADGCRQAGCALAGGETAEMPGLYAKGDFD
                     LAGFCVGVVEAQQIIDGSRVTPGDAVIALPSSGAHSNGYSLIRKIIEHSAADLQQPLD
                     GQPLIDRLLAPTRIYVRPILALIRTLEVKAIAHITGGGLIENIPRVLPQDTQAVIERA
                     SWQRPALFDWLQQQGAVEESEMLRTFNCGVGLVLVVAAENAEAAMNQLRQSGEAPWQI
                     GRIAACAPQQPAVQLV"
     gene            8750..9421
                     /gene="purN"
                     /locus_tag="PKI23_06435"
     CDS             8750..9421
                     /gene="purN"
                     /locus_tag="PKI23_06435"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNN36610.1"
                     /translation="MPRLVLLLSGAGSNLQAIIDAIEAGQLPARVVAVISNRADAPGL
                     QRAARHGIPSQVISHRDHPDRASFDAALASAIDHYQPDWVLLAGFMRILTPQFVDHFR
                     DRLLNIHPSLLPAYPGLDTHRRVLAAGEREHGATLHLVTEALDAGPTVLQGRFAVEAD
                     DDAATLAQKVHRLEHQIYPAALALLVSGQLRIDQGRLFYLDQPVPAEGLQWDQVLGSV
                     TPRCP"
     gene            complement(9385..9951)
                     /gene="dcd"
                     /locus_tag="PKI23_06440"
     CDS             complement(9385..9951)
                     /gene="dcd"
                     /locus_tag="PKI23_06440"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNN36611.1"
                     /translation="MSIKSDRWIRRMAQDEGMISPFEPGQVREQQGRRIISYGTSSYG
                     YDVRCADEFKIFTNLFSVTVDPKNFDERSFVDFSGEVCVIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTNLPAKIYANEGVAQMLFF
                     ESDEVCEVSYRDRGGKYQGQRGVTLPKT"
     gene            complement(9958..11052)
                     /gene="apbC"
                     /locus_tag="PKI23_06445"
     CDS             complement(9958..11052)
                     /gene="apbC"
                     /locus_tag="PKI23_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNN36612.1"
                     /translation="MAELQAAALQALASYRDPYLDAGLEALKAITQLEIVATVARLKV
                     ELGYPAAGLRQAMITELRDRLLGVAGITAADVEIGWRIHAHQSSSKGNELPSVSNLIA
                     VASGKGGVGKSTTAVNLALALQAEGATVGLLDADIYGPSQAVMLGIADGTRPEVVEQK
                     YFLPVMAHGLQTMSMAFLANDKTPMVWRGPMASGALQQLLKQTLWRDLDYLLVDMPPG
                     TGDIQLTLAQQAAVTGAVIVTTPQEVATLDARRGIEMFRKVNIPVLGIVENMAVHICS
                     HCGHHEHLFGEGGGERVAREYRAHLLGSLPLDLRIREQTDGGRPTVVAEPDGELAATY
                     RHIARAVAAQVARLPRAAAQRFPTISVQGD"
     gene            complement(11142..11561)
                     /gene="msrB"
                     /locus_tag="PKI23_06450"
     CDS             complement(11142..11561)
                     /gene="msrB"
                     /locus_tag="PKI23_06450"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNN36613.1"
                     /translation="MSDEQQQDWRSRSDAQWQQQLDADAFAVCRRSATEHPFSGRFWN
                     HHEAGHYHCICCGMALFSSEHKYDSGCGWPSFYEAIDRARIEELPDFSHGRYRIEVRC
                     LRCAAHLGHRFDDGPPPTGDRYCINSVALDFVPAAST"
     gene            11660..12871
                     /locus_tag="PKI23_06455"
     CDS             11660..12871
                     /locus_tag="PKI23_06455"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNN36614.1"
                     /translation="MDIKKSRKLANVCYDIRGPVQRQALRLEEEGHRILKLNIGNPAA
                     FGFEAPEEIVRDVIHNLPEAQGYSDSKGIFAARKAVMQESQRLGINDVTIDSIILGNG
                     VSELIVMAMQALLNNGDEMLLPAPDYPLWTAAVTLSGGTPVHYLCDEQSDWYPDLEDM
                     ERKITSRTRGIVVINPNNPTGAVYPREILEGIVELARRHQLIIFADEIYDKILYDGAV
                     HHPLASLADDLLFITFNGLSKSYRAAGFRSGWMIISGAKHRAVDFLEGLEILASMRLC
                     ANVPSQFAIQTALGGYQSINDLILPGGRLRAQRDCAWELFNSIPGISCVKPKGALYLF
                     PRIDPARHKILNDEKMVLDLLLQEKILLVQGTAFNWPDTQHFRVVFLPREDVLEQAAG
                     KVAKFLGHYSQ"
     gene            12952..15015
                     /gene="ppk1"
                     /locus_tag="PKI23_06460"
     CDS             12952..15015
                     /gene="ppk1"
                     /locus_tag="PKI23_06460"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNN36615.1"
                     /translation="MQPQISTRPDLVAKELSWLSFNERVLQEAKDRTVPLIERIRFLG
                     IFSSNLDEFFRVRVAELKRLILIENEGETHDAGERLLKEIQLKLHGLQEQFDEVYRQI
                     LLELARRNIFLVNEQQLSARQGEWVRQFFQERIALVLSPILLDEEDELPSLQDDLIYL
                     AIDLRLVDGRQLYGLLEVPTRQFGRFIPIPVDRAPRKKSYIILDNIIRYCLREIYQKV
                     FDVESVAAYTVKVTRDAELELSEGISQSLLDKLSSSLKRRLSAEPVRLVYDREMPARM
                     TNFFIRKLKLSSYDSMIPGGRYHNFKDFIDFPDVGRRGMLNPPLPELRSAAFDRHRNS
                     FDAIAAGDILLYYPYHSFRHFTDWLREAAIDPQVTRIAISLYRVARNSQVVNAMMTAA
                     RNGKQVKVIVELRARFDEQANIEWAKTLTEAGVEVEFGIPTLKVHSKICLIKRLEAGR
                     EVGYAHIGTGNFHERTARTYTDFSLFTTHPGITNELDQLFEFISHSYKRFEFRHLLIS
                     PLTMRDSVTRLIQREINIARAGRAAQITLKVNNLVDERIIDQLYTASQAGVTIRLIVR
                     GMCALKPGIAGLSENISAISIVDRFLEHPRVLIFGNDGDPDLFISSADLMARNLDGRV
                     EVGCPIYDPALKQRVLDIIALQLNDTVKARLIDPQQRNDYVERVGRRKIRSQLAIFDH
                     LKKNP"
     gene            complement(15088..16458)
                     /locus_tag="PKI23_06465"
     CDS             complement(15088..16458)
                     /locus_tag="PKI23_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNN36616.1"
                     /translation="MENVFSRGAVAASLLLALLPLSAQAKVSPEEAAKLGIEGTELTP
                     AGAVRAGNADGTIPAWSGGLPQKDVPKGKWLDDPYADDKPLFTITAQNYQKYADKLTI
                     GQKAMFERHPGYKMNIYPTRRSHSNKKYIYEAAIANASRAELTETRWDYKHAINTWAF
                     PIPKDGYEAVLNFATKPFPSVFRWNNTTVVTSNGDFQTTKITEEFVTDWSKEGMNPDE
                     FTGDKDVGYFLQSVVAPAKVAGSVILIHIVFHWDQHDMIAWSYNPGQRRVRRAPQIRY
                     DNPKTGADGLATSDQLGMYGGKIDRYNWKLVGKKEIYIPYNSYKLHTDKVKIADIIQK
                     EHINQDLARYELHRVWQVEGDLLPDTSHIYKKRVFFMDEDSWQIVLMDHYDKRDQLWR
                     FSEGHALNHYDIQHTAPTADVHYDLQSGRYLVIGLDNEDAPPDYTFTATADRYSPDRL
                     RGEGVR"
BASE COUNT         3042 a         5498 c         5055 g         3086 t
ORIGIN      
        1 ccgcgccctg cgccttcaac ccccgggcga gcgaaccgcc gatcaggccg acgccgagaa
       61 tggcaacgcg acgccaatgg ggttgcgcgg ctccagcgtc cattcaaccg gcttccagaa
      121 cttcttgcag cgccttgatg aagcggctgt tttctgcttc ggtcgatgcc gtgacccgca
      181 ggtggttcgg catgccatac ccggccagcg gacggacgat cagccccttg cgcaacagtg
      241 cttcatagat cggcagcgcc acccgcccca tgtcgatggc gatgaagttg ccaaccgagg
      301 ggatggctcg aagtccgagc cgttcggccg cttcgcgcaa ctgcaccaat ccctgctggt
      361 tcaccgcttt gctgcgttgc aggtgggctt catcggccag cgcggcctcg gcggccgcca
      421 gtgcgatgct gttgacgttg aagggttcgc ggatgcggtt catcagatcg gccgtcgccg
      481 ggtcggcaat cgcatagccg acccgcaatg ccgccagacc ataggccttg gagaaggtgc
      541 gcatgacgat caggttggga aactccagcc ccagcgccat gccattggga tagtcgggct
      601 ggtcgatgta ttcggcatag gcctcgtcca gcacgaccag cacctgctgc ggcagccgct
      661 tcaacagccg cagcagatcg gcctgcccga cccaggtgcc ggtcgggttg ttggggttgg
      721 cgatgaacag catccgcgtg cgatcagtca ctgcagccgc catggcatcg agatcgtgcc
      781 cccagtcacg ggccggcacc acgatgcgct ccgcctgtgc cgcctgggca tagatggcat
      841 acatggcgaa ggcgtattgc gatatcacca cctgatcacc ggcgcagacg aaggcttcga
      901 tcgccagccg caacaggtca ctggagccgg cgccaagcac caaccgccgc tgatcgagcg
      961 cgaacttgcg cgccaatgcc gccttgagcc gaaaaccggc gccatcggga tagcggctca
     1021 gctcactcag cgccgcccgg gccgcttcgc tggcctgcgg gctggcgccc agcggatttt
     1081 cgttgctggc cagtttgatg ctgccggtga tgccaagctc gcgctccagt tcatcgatcg
     1141 gcttgcccgg ttcatacggg gtgaggtcac gcacgccggg attggccaac tggtaaaaat
     1201 cacagctcat ggtcgaggtt ccggatcgcg gttacaggtc gctgccgttg ctggcacgtg
     1261 gataggagcc cagccacttc acctcggcgc tgtgctgctc gatctcttca agcagcgcgg
     1321 cgatgtcggg ctccaggcgg tgtccttcga aatcgatgaa aaagcggtag cgccagggat
     1381 ggctcttggc cggtcggctc tccagccggg tcagactgat gccccgatgc tcgaagggtg
     1441 ccagcagtcg atacagcgct ccggggcggt tcggcaccga gaccagcaga ctggtcttgt
     1501 catggccgct gatgccggga ggttgctggc cgagcaccag aaaacgggtg gtgttgctgg
     1561 aaaaatcctc gatccgctcg gccagcacct gcaatccata gtgctccgca gccatcttgc
     1621 cggcgatggc caccacctgc ggatcttcgc tggccagccg ggccgcttcg ctgttgctgc
     1681 tgacggcctg ctgttcgaca cccggcagat gggcgttgag ccagttgcgg cactgtccaa
     1741 gcgactgttg gtgcgagagc acccgccggg gggtgccgtg cgcctggcga cccagcagac
     1801 agtgctgcac cgccagttcg acctcgccac agagcgtcag cgcggtgtcg acgaaaagat
     1861 cgagtgtctg accgaccatg ccttcggtcg agttctcgac cggcaccagg ccataatcgg
     1921 ccaggccacg ctcgacctga gcgaagatgt cggcgatggt cggctgcgcc atgccttcga
     1981 tcgcctgacc gaaatgtttg acggccgccg cctcgctgaa ggtgccagcg ggacccaggt
     2041 aggccacccg cagtggtttc tccagcgcca gacaggccga catcagctca cgaaacagca
     2101 gggccaccgc cgcattgggc agtggaccag ggttgttctg ttgcacctgc gccagaatct
     2161 gcgcctcgcg ctcgggtcga tggaagtggc ttggcacacc ctcggcgtgg gcgatcttga
     2221 tcgccgccac ctgctgggcg cactgcgccc gtgcattgat cagccgcaac agttcgctgt
     2281 caatcgaatc gatctgctgg cgaaccgccg ccagttgtcg tgcctcttca tcctgacgct
     2341 ggctcatggt gcatcacccg ttacgccggg caaaatcgct catgtagtcg atcagcgcat
     2401 ccacggcggc ctcgggcacg gcattgtaga gactcgcccg catgccaccg accgagcggt
     2461 gtcccttcag gttcagcagc ccggcggcat ccgccccttt caggaaggcg ccatccagtt
     2521 ttgcgtcggc cagaatcatc ggcacattca tccaggaacg gcaggcggga tcgatcggat
     2581 tgcgatagaa accgctgcca tcgaggtagc gatagagttt ttcggccttg cgccggttga
     2641 cctcgccgat ggcggtcagg ccaccctgtt cgagcagcca ctcgaacacc agtccggcca
     2701 gataccaggc ataggtgggc ggcgtgttgt acatcgactc attcttggcg gtcagccgat
     2761 agtcgagcac catcggaatg gtggcggtgg cccgctccag cagatcttca cggatgatca
     2821 ccagggtcag accggccgga ccgatgttct tctgcgcacc ggcgtagatc accccgaagc
     2881 ggctgacatc gagtggtcgc gagagaatcg tcgatgacat gtcggccacc agcggcaccg
     2941 caccggtatc gggaatgaag gaaaactcga ccccgccgat ggtctcgttg ggggtgtagt
     3001 ggcagtaggc cgccgtggca tcgagccgcc actccgccag cggcgggatg gtggtgaact
     3061 tttcggcctc gctgctggcg gcctcgttga ccctgccgaa gcggcgcccc tcttcgatcg
     3121 ccttctgcga ccagctgccg gtgttgatgt agtcggcatg gccggtggcg ccgagcagat
     3181 tgagcggcac ggcagaaaac tgggcggtgg cgccgccctg catgaacagc acccgatagt
     3241 gggcgggaat gtcgagcagc cggcgcaggt tggcctcggc ctgtgccgcg atggcgatga
     3301 actcatcgct gcgatggctc atctccatga ccgacaggcc acgcccctgg tagtcgagca
     3361 tctccgcctg cgccttgcgc agaaccggtt cggggagcgc agcgggaccc gcgcagaagt
     3421 tgtagactct tgccatcttc accacatcgt tcaggttggg accggcgcag gggccggtcg
     3481 gttgaaaacg ccgctactgg agcgcctcgc cgtcatcgga ctcttcgtca ccctcgtctt
     3541 cggcaatgcg ctcgaccccc accagatgct caccctcggc cagcttgatc agtcgtactc
     3601 cctgggtgtt tcggcccagc agcggcgttt cgtcacagcg ggtgcgcacc agcgtgccct
     3661 ggtccgagat cagcatgatg tggtccttgt cgcagacctg gatcacgctg accagtgcac
     3721 cattgcgctc gctcagctgc atcgagatga ccccttggcc accacggcca tggagcggga
     3781 attcggcaat cgcgctgcgc ttgccataac cgtttagact cgccatcaac agttcgccat
     3841 cggccgccgg cacgatcagc gcgatgaccc gctgcgcatc gttcagccgg atgccacgca
     3901 cgccacgtgc cgtgcgcccc atcggccgca cctgcgcctc ctgaaaacgg atcgccttgc
     3961 catcgctgct gaacagcatc acttcgccat tgccatcggt caaggccgca cccaccagcg
     4021 tgtcgctctc gtcgagatcg atggcgcgaa tgccgagcga gcgcggacgg gagaactcca
     4081 ccagcggggt cttcttgacg gtgccatcgg ccgtcgccat gaagacatag cgatcttctg
     4141 gatattcacg aatcggcagc accgcagtga tgcgttcatg ctcgctcaat ggcagcaggt
     4201 tgacgatcgg ccggccacgc gaggcccggc tggccaccgg aatctgatag accttgatcc
     4261 agtagatttt gcccagcgtg ctgaagcaga gcagggtgtc gtggctgctg gcaaccagca
     4321 gggtttcgat gaagtcatcc tccttcaccc cggtggcact cttgccgcgg ccaccgcgct
     4381 tctgcgcctg ataggcatcc agcggctggc tcttggcata gccgccatgc gagaagatca
     4441 ccacccgctc ctcttcgctg atcagatcct cggtggtcag gtcgagctgg tcgagcagaa
     4501 tctcggtgcg gcgggcatcg ccatactggt tgcggatctc ggtcagctcc tggcggatca
     4561 cctcgatcaa ccgcgccgtg ctgccgagaa tctcctcgta gttggcgatg ttgccaagcg
     4621 tctcctgata ttcactgcgc agcttgtcat gctcgagacc ggtcagacgg tgcagccgca
     4681 gatcgagaat cgcctgcgcc tgctccggcg agagccggta gctttcgcca tcgaggccat
     4741 actccgctgg cagaccatcg ggacgacagg cgctggcgcc ggcctgctcc agcagtgcca
     4801 ccacctcact ggcctgccag ggctgggcga ccagcgcctc tctggccagc gccggcgtgg
     4861 cggagttgcg gatcagctcg atcaccgcat cgatgttggc cagcgccacc gacagccctt
     4921 cgaggatgtg gccacgctca cgcgccttgc gcagcagaaa gagggtgcgc cgggtcacca
     4981 cctgacgacg atggcgcacg aaggcttcga gcatctcctt gaggttgagc acgccgggac
     5041 ggccatcgac cagggcgaca cagttgatgc cgaagaccgt ctccatctgc gtctgggcat
     5101 agaggttgtt gagcaccacc tcgcccacct cgccgcgccg cagctcgatg acgatgcgca
     5161 tgccatcctt gtcggactca tcgcgcagct cgctgatgcc ttcgagcttt ttctcccgca
     5221 ccagctcggc gatcttctcg atcagccgcg ccttgttcac ctggtagggg atctcctcga
     5281 cgatgatgac ctcgcggcca ttcttgctgt cgttctcgat gcgggcacgg gcacggatgc
     5341 ggacacggcc gcgcccggtg cggtaagcct cgatgatgcc ggcacggcca ttgatcacgc
     5401 cggcggtcgg aaagtccggc ccgggaatcg actccatcag cgcatcgatg gtgatgtcgg
     5461 gatcatcgat cagcgccaga cagccgttga ccacctcggt gaggttgtgc ggcggaatgt
     5521 tggtggccat accgaccgcg atgccggatg agccgttgat caacaggttg ggcaccttgg
     5581 tcggcagcac ctcgggaatc tgctcggtgc catcgtagtt ggggacgaag tcgaccgtct
     5641 ccttgtcgat gtcggcgagc agctcatggg ccagccggct catgcggatc tcggtgtaac
     5701 gcatggccgc tggcgagtcg ccgtcgatcg aaccgaagtt gccctggcca tcaaccagtg
     5761 gatagcgcag cgagaaggtc tgcgccatcc gcacgatggt gtcgtagacc gccaaatcac
     5821 cgtgcgggtg atatttgccg atgacgtcac cgaccacccg ggccgacttc ttgtaggcct
     5881 tgttccagtc gttgccgagc tcgttcatcg cgtgcagcac gcgccggtga accggcttca
     5941 ggccatcgcg cacatcgggc agtgcccggc cgacgatcac gctcatcgca tagtcgaggt
     6001 aggactgctt gagctcatcc tcgatgctga ccggcactat ctctttggct gactcaccca
     6061 tggtttctgc ttgttttcaa ctgtgtattg acccgcaacg gcacgcgccc gattcaacat
     6121 gacccgccgc catcaaagcc cggcatcata ccacagcggc cggcactgcc gcccgcggcc
     6181 gactcgattt ccccccgaca aatcaatatc ctcaacacac ctgtttccct gccggagtga
     6241 ccccgatgtc tgccctgccc gccgatgcgc cccaccttca gcagatcgac ctgctgatcg
     6301 aggcgcgctg gatcatcccg atcatccccg cgggagaggt gttggagcat caggcgatcg
     6361 ccatcgatca gggagcgatt cgcgatctgc ttcccagcac cgaggcacgg cagaaatacc
     6421 gcccccgcca gcatctgaca ctgcatgagc atgtcctgct gccgggcctg gtcaatgccc
     6481 atggccatgc cgccatgtcg ctgtttcgcg gtctggccga cgacctgccg atgatggagt
     6541 ggctgaccga ccacatctgg cccgccgagc agcactgggt cagcgaaacc ttcgtcgagg
     6601 atggcaccga gctggccatc gccgagatgc tccgttccgg caccagctgc ttcgccgaca
     6661 tgtatttctt ccccgaccgg gtggcccggg tcgccagccg cagcggcatc cgggcccagg
     6721 tctgctttcc ggtgctcgac ttcccgaccc tctgggcgca gcaggccgac gactacctgc
     6781 acaaggggct gatgctgcac gatgactacc gcgaccatcc acgggtgcgg atcgccttcg
     6841 ggccccatgc cccctactcg gtcagcgacg ccccgctgag ccgcatcgcc accctggccg
     6901 aggagctcga cctgtcgatc cagatccacc tgcacgaaac cgcgcaggag gtcgaggagg
     6961 ccatggcccg cagcggccgt cgaccgctga tgcgcctggg cgagctggga ctgctctccc
     7021 cgcgcctgca ggcggtgcac atgacccagc tgctcgacag cgagatcgag ctgctggccc
     7081 gccatggcat ccatgtcatc cactgtcccc gctccaacct caagctggcc agcggcatgg
     7141 cgccggtggc ccggctgctc gatgccggcg tcaatgtcgc gctgggcacc gacagcgccg
     7201 ccagcaacaa ctcgctcaac ctgttcgacg aactggcgat ggcggccctg ctcggcaagg
     7261 gggtcagtgg cgacgcccgc gccatcgatg cccaccgggc actcgaactg gccacgctgg
     7321 gcggcgcccg cgcactcggc ctcggtgagc agatcggttc gctcgaagcg ggcaagctgg
     7381 cggatctgat cgcggtcagg ctcgacgaca tcaaccagca gccgctctac caccccgcct
     7441 cgcaactggt ctacagcgcc agcggcaacc gggtcagcca cctgtgggtg gccggacgcc
     7501 cgttgttgag tgatggccag ttgcagagca tcgaccttgc atcactgcgc gaacgggtga
     7561 atggctgggc cgcccggatc ggcaccgacg gcatcaaggg tcgtccgtga agcgcgaaca
     7621 gccctgccgc tctgctgcta caatgcgcgt cccgctcgat ccgactccgc aggtacacca
     7681 tgcaccccaa ggcgcccttc tctacccatg gcctcagtta ccgcgacgcc ggtgtcgaca
     7741 tcgacagcgg caacgcgctg gtcgagcgca ttcggccgct ggcgcgcagc acggcccggc
     7801 cggagctgct gggcggcatc ggtggtttcg gggcgctctg ccggttgccg gccggctacc
     7861 gcgaaccggt actggtgacc ggcactgacg gcgtcggcac caagctcaag ctggcgcagc
     7921 aggccgatcg gctcgaaggg ctcggcatcg atctggtggc gatgtgcgtc aacgacctga
     7981 tcgtctgtgg cgccgaaccg ctgctgtttc ttgactatta cgccaccggc aagctcgatc
     8041 tggagagtgc gacccgcatc atcagcggca tcgccgatgg ctgccggcag gcgggatgcg
     8101 cgctggccgg cggcgagacc gccgagatgc cgggactcta cgccaagggt gactttgacc
     8161 tggccggctt ctgcgtcggc gtggtcgagg cgcagcagat catcgacggc agccgggtca
     8221 cgccgggcga tgccgtgatc gccctgccct catcgggcgc ccactccaac ggctattcgc
     8281 tgatccgcaa gatcatcgaa cacagcgcag ccgacttgca gcaacctctc gacggccagc
     8341 cactgatcga ccgactgctc gccccgaccc gcatctatgt gcggccgatt ctggcgctga
     8401 tccgcacgct ggaagtgaag gccatcgccc acatcaccgg cggcggtctg atcgagaaca
     8461 tcccccgggt attgccgcag gacacacagg cggtgatcga acgtgcaagc tggcagcgcc
     8521 cggcgctgtt cgactggctg cagcagcagg gagccgtcga ggagagcgag atgctccgca
     8581 ccttcaactg cggtgtcggg ctggtgctgg tggtggcggc ggaaaacgcc gaggcggcca
     8641 tgaaccagtt gcggcagagt ggcgaggcac cctggcagat tggccgaatc gccgcctgcg
     8701 ccccgcagca acccgccgtt cagctcgttt gacccgccac gacaggccac tgccgcgact
     8761 ggtgctgctg ctctcaggcg ccggcagcaa cctgcaggcg atcatcgatg cgatcgaggc
     8821 cggccaactg ccggccagag tggtggcagt gatcagcaac cgcgccgatg cccccgggtt
     8881 gcagcgggcg gcacgacatg gcatccccag ccaggtgatc agccaccgtg accaccccga
     8941 ccgcgccagc ttcgatgccg ccctggcgag cgccatcgac cactatcaac ccgactgggt
     9001 gctgctggcc ggcttcatgc gcattctcac accacagttc gtggaccatt tccgcgaccg
     9061 gctgctcaac atccatcctt cgctgctgcc cgcctatccc ggcctcgaca cccaccgacg
     9121 ggtgctggct gccggcgagc gcgagcatgg cgccaccctg catctggtga ccgaagcact
     9181 ggacgccgga cccaccgtgc tgcaagggcg ttttgccgtc gaggcggacg atgatgccgc
     9241 cacgctggcg cagaaggtgc accggctgga gcatcagatc tatccggccg cgctggcgct
     9301 gctggtcagc ggtcagttgc gcatcgacca gggccggctc ttctacctgg atcagccggt
     9361 accggccgag ggattgcagt gggatcaggt cttgggcagc gtgacgccgc gctgcccctg
     9421 atacttgccg ccccggtcgc ggtagctcac ctcacagact tcatccgatt cgaagaagag
     9481 catctgcgcc acgccctcgt tggcgtagat cttggccggc aggttggtgg tgttggagaa
     9541 ctccagggtc acatgcccct cccactccgg ttcgagcggg gtcacgttga cgatgatgcc
     9601 gcagcgggca taggtcgact tgcccagaca gacggtgagc acgttgcgcg ggatgcggaa
     9661 atactccacc gtgcgcgcca gcgcgaagga gttgggtgga atcacgcaga cctcgccgct
     9721 gaagtcgaca aaactgcgct catcgaaatt cttcggatcg accgtcaccg agaagaggtt
     9781 ggtgaagatc ttgaactcat cggcacagcg cacatcgtag ccatagctcg aggtgccgta
     9841 ggagatgatc cgtcgcccct gctgctcgcg cacctgtccc ggctcgaatg gcgagatcat
     9901 cccctcgtcc tgcgccatgc ggcgaatcca gcggtccgat ttgatgctca tgaagactca
     9961 atcaccctga acggagatgg tgggaaaacg ctgggccgct gcacgcggca gccgcgccac
    10021 ctgggctgcc acggcgcggg cgatgtggcg ataggtcgcc gccagttcgc cgtcgggctc
    10081 ggcgaccacg gtcggccgac cgccatcggt ctgctcgcga atgcgcagat cgagcggcag
    10141 cgagccaagc agatgagcac ggtattcacg cgccacccgt tcgccgcccc cttcgccaaa
    10201 cagatgctca tgatggccgc agtggctaca gatgtgcacc gccatgtttt cgacgatgcc
    10261 gagcaccggg atgttcacct tgcggaacat ctcgatgccg cgccgggcgt cgagggtggc
    10321 cacctcctgc ggcgtcgtca cgatcaccgc gccggtcact gccgcctgct gcgccagcgt
    10381 caactgaatg tcaccggtac cgggcggcat gtccaccagc agatagtcga gatcgcgcca
    10441 gagggtctgc ttcaacagct gttgcagggc accgctggcc atcggtccac gccagaccat
    10501 cggtgtcttg tcattggcca gaaaggccat cgacatcgtt tgcagaccat gggccatcac
    10561 cggcagaaaa tatttctgct ccaccacctc ggggcgggtt ccatccgcga tgcccagcat
    10621 cactgcctga ctggggccat agatgtcggc gtcgagcaag ccgaccgtgg cgccctcggc
    10681 ctgcaacgcc agcgccaggt tgaccgcggt ggtcgacttg ccgaccccgc ccttgccgga
    10741 ggcaacggcg atgaggttgc tgaccgaagg cagctcattg cccttgctgc tgctctggtg
    10801 cgcatggatg cgccagccga tctccacgtc ggcggcggtg atgcccgcca cgccaagcag
    10861 gcgatcgcgc agttcggtga tcattgcctg tcgcagcccg gccgccggat agccaagctc
    10921 caccttgagc cgggccactg tggccacgat ctccaactgc gtgatcgcct tcagcgcttc
    10981 cagacccgcg tcgagatagg gatcacgata actcgccagc gcctgtaatg ccgcagcctg
    11041 caactccgcc atcaccttct ccaccgccga ttgaagccaa aaagggggcc aatcttaatg
    11101 aaaagccgtc aaggcgcata gcgccgccct ttggcagcag atcatgtcga tgccgccggc
    11161 acaaaatcga gcgccaccga gttgatgcag tagcggtcgc cggtgggcgg tgggccgtca
    11221 tcgaaacggt gccccaggtg ggccgcacag cgcaggcagc gcacctcgat gcggtagcgg
    11281 ccatgactga aatcgggcaa ctcttcgatg cgggcgcggt caatggcttc gtagaagctg
    11341 ggccagccgc agccggagtc atacttgtgt tcggacgaga acagcgccat cccgcagcag
    11401 atgcagtggt aatggcccgc ctcatggtgg ttccagaaac gaccggaaaa ggggtgctcg
    11461 gtggcactgc gccggcagac ggcgaaggca tcggcatcga gctgctgctg ccactgggca
    11521 tcgctgcggc ttcgccaatc ctgctgctgt tcatcgctca tgcgggactc ctcgggtttt
    11581 gccaaaaagc atatccggca cggtccaaaa accgctagaa tgctttttga ttatttaaaa
    11641 aatcgctgac aacatcatga ttgacattaa aaaatcgaga aaactcgcca acgtctgcta
    11701 cgacattcgt ggtccggtac agcgccaggc gctgcgcctt gaagaagagg gtcaccgcat
    11761 cctcaagctg aacatcggca atccggcggc attcggcttc gaagcgcccg aagagatcgt
    11821 gcgcgatgtc atccacaacc tgcccgaggc gcaaggctac agcgactcca agggcatctt
    11881 cgccgcccgc aaggcggtga tgcaagagag ccagcggctc ggcatcaatg atgtcaccat
    11941 cgacagcatc attctcggca atggcgtcag cgagctgatc gtgatggcca tgcaggcgct
    12001 gctcaacaat ggcgacgaga tgctgctgcc ggcacccgac tacccgctgt ggaccgccgc
    12061 cgtcaccctc tccggcggca cgccggtgca ctacctgtgc gacgagcagt ccgactggta
    12121 tcccgacctg gaggacatgg agcgcaagat caccagccgt acccgcggca tcgtggtgat
    12181 caaccccaac aaccccaccg gggcggtcta tccgcgcgag attctcgaag gcatcgtcga
    12241 actggcgcgc cgccatcaac tgatcatctt cgccgacgag atctacgaca agattctcta
    12301 tgacggcgcg gtgcaccatc cactcgccag cttggccgac gacctgctgt tcatcacctt
    12361 caacggcctc tccaaatcct accgggcggc cggattccgc tccggctgga tgatcatcag
    12421 tggcgccaag caccgcgccg tagattttct cgaaggcctg gagattctgg cctcgatgcg
    12481 gctgtgcgcc aatgtgccgt cacagttcgc gatccagacc gcgctgggtg gttatcagag
    12541 catcaatgat ctgatcctgc ccgggggccg gctgcgcgcc cagcgtgact gcgcctggga
    12601 gctgttcaac tcgatcccgg gcatctcctg tgtcaagccg aagggggcgc tctacctgtt
    12661 tccccgcatc gatccggcgc gccacaagat tctcaatgac gagaagatgg tgctcgatct
    12721 gctgctgcag gagaagatcc tgctggtgca gggcaccgcc ttcaactggc ccgacaccca
    12781 gcattttcgc gtcgtctttc tccccaggga ggatgtgctg gagcaggcgg ccggcaaggt
    12841 ggccaagttt ctcggccact attcccaatg agactgtcat gcaaccgtca tgcgtcagcc
    12901 attgaatggc cgccgcccaa tccagcgcga tccgtttgat ccgacccgca catgcaacca
    12961 cagatttcca cccgtcctga tctggtcgcg aaggagctca gctggctcag cttcaacgaa
    13021 cgggtgttgc aggaggcaaa ggaccgcacc gtgccgctga tcgagcgcat ccgctttctc
    13081 ggcatcttct ccagcaacct cgatgaattt ttccgcgtac gtgtcgcgga gctgaaacgg
    13141 ctgatcctga tcgagaacga aggcgagacc cacgacgccg gcgagcggct gctcaaggag
    13201 attcagctca agctgcatgg cctgcaggag cagttcgatg aggtgtaccg gcagattctg
    13261 ctcgaactgg cgcgccgcaa catctttctg gtcaatgagc agcagctcag cgccagacag
    13321 ggcgagtggg tccggcagtt ctttcaggaa cggatcgcgc tggtgctgtc gccgatcctg
    13381 ctcgacgaag aggacgaact gccatcgctg caggatgacc tgatctacct ggccatcgat
    13441 ctgcggctcg tcgacggccg tcagctctac ggtctgctgg aggtgccgac ccgacagttc
    13501 ggtcgcttca ttccgatacc ggtcgaccgg gcgccacgca agaagagcta catcatcctc
    13561 gacaacatca tccgctactg cctgcgcgaa atttaccaga aggtcttcga tgtcgaatcg
    13621 gtcgccgcct acaccgtcaa ggtgacgcgt gacgccgaac tcgaactgag cgagggcatc
    13681 tcgcagagcc tgctcgacaa gctgtcgagc agcctcaagc ggcgactgag cgccgaaccg
    13741 gtccgactgg tctacgaccg cgagatgccg gcacggatga ccaacttctt catccgcaag
    13801 ctgaagctca gctcctacga cagcatgatt cccggtgggc gctaccacaa cttcaaggat
    13861 ttcatcgact ttccggatgt cggccggcgc ggcatgctca atccgcccct gcctgaactg
    13921 cgctccgctg ccttcgaccg gcaccgcaac agcttcgacg ccatcgccgc cggcgacatt
    13981 ctgctctact acccctatca cagctttcgc cacttcaccg actggctgcg cgaggcagcg
    14041 atcgacccgc aggtcacccg catcgccatc tcactctatc gagtggccag aaattcgcag
    14101 gtcgtcaatg cgatgatgac cgccgcgaga aatggcaagc aggtgaaggt gatcgtcgaa
    14161 ctgcgtgccc gctttgatga gcaggccaac atcgaatggg ccaaaacttt gaccgaagct
    14221 ggtgtggagg tcgaattcgg cattccgaca ctcaaggtgc acagcaaaat ctgcctgatc
    14281 aagcggctcg aagcgggccg tgaagtgggc tatgcccaca tcggcaccgg caacttccat
    14341 gaacggaccg cccgcaccta caccgacttc agcctcttca ccacccatcc cggcattacc
    14401 aacgagctcg accagctgtt cgagttcatc agccacagtt acaaaaggtt cgaattccgt
    14461 catctgctga tctcgccgct gacgatgcgc gacagcgtga cccggctgat tcaacgcgaa
    14521 atcaacatcg cccgcgccgg tcgggcagcc cagatcacgc tgaaggtcaa caacctggtg
    14581 gatgagcgga tcatcgatca gctctacacc gccagtcagg ccggcgtcac catccggctg
    14641 atcgtgcgcg gcatgtgcgc cctcaagccc ggcattgccg gattgagcga aaacatcagc
    14701 gccatcagca tcgtcgaccg cttccttgaa cacccacggg tgctgatctt cggcaatgat
    14761 ggcgacccgg atctcttcat ctcctccgcc gacctgatgg cacgcaacct ggatggacgg
    14821 gtggaggtcg gctgcccgat ctacgatccg gctctgaagc agcgggtgct cgacatcatt
    14881 gcgctgcaac tgaacgatac ggtcaaggca cgtctcatcg atccgcagca acgcaatgat
    14941 tatgtcgagc gggtcggccg caggaagatc cgttcgcagc tggcgatttt cgaccacctg
    15001 aagaagaacc cctgagcggt tcctgttggg caatgtggcc accagcgggc tctgtgcagc
    15061 accgctggtg gccactgttc aacccggtca gcgcacccct tcaccacgca gccggtccgg
    15121 cgagtagcga tcggccgtgg cggtgaaggt gtagtcgggc ggagcatcct cgttgtccag
    15181 accaatcacc aggtagcgcc cagattgcag gtcatagtgc acatctgccg tgggggcggt
    15241 gtgctggatg tcatagtggt tgagcgcatg cccttccgag aagcgccaga gctgatcgcg
    15301 cttgtcgtag tggtccatca gcacgatctg ccaggagtcc tcatccatga agaagacccg
    15361 cttcttgtag atgtgactgg tgtcgggcaa cagatcgccc tcgacctgcc agacccggtg
    15421 cagttcatag cgggccagat cctgattgat gtgctccttc tgaatgatgt cggcaatctt
    15481 caccttgtca gtgtgcaatt tgtaggagtt gtaggggatg tagatctcct tcttgccgac
    15541 cagcttccag ttgtaacggt cgatcttgcc gccatacatg cccaactggt cactggtggc
    15601 caggccatcg gcaccggtct tggggttgtc gtagcgaatc tgcggcgccc gccgcacccg
    15661 ccgctgaccc gggttgtagg accaggcgat catgtcatgc tgatcccagt ggaaaacgat
    15721 gtggatcaga atgaccgaac cggccacctt cgccggcgcc accaccgatt gcaggaagta
    15781 gccaacatcc ttgtcaccgg tgaactcatc gggattcatc ccctccttcg accagtcggt
    15841 cacgaactcc tcggtgatct tggtggtctg gaagtcgcca ttcgaagtga ccaccgtggt
    15901 gttgttccag cggaatacgc tgggaaacgg cttggtggcg aaattcagca ccgcctcata
    15961 gccatccttc ggaatcggga acgcccaagt attgatcgca tgcttgtaat cccacctggt
    16021 ttcggtcagc tcagcgcgcg aggcattggc gatggccgcc tcgtagatgt atttcttgtt
    16081 gctgtgcgag cgccgggtcg gatagatgtt catcttgtag cccggatggc gttcgaacat
    16141 tgccttctgc ccgatcgtca acttgtcggc atacttctgg tagttctgcg cggtgatggt
    16201 gaacagcggc ttgtcatcgg cgtaaggatc atcgagccac ttgcccttgg ggacatcctt
    16261 ctgtggcagg ccaccactcc aggccggaat ggtgccatcg gcattgccgg cacgcaccgc
    16321 cccggcaggg gtcagttcag tgccctcgat gcccagcttg gccgcttcct caggcgagac
    16381 cttcgcctgc gcgctcagag gcaacagcgc cagcagcagc gacgctgcaa ccgcaccacg
    16441 gctgaagaca ttctccatat ccttctccct gtttcattat tatcgatacg ttcgtctctt
    16501 cttttttcgc tctgccgcaa tccacgcttg ccgaatgtcg gcaaaccatg aattttggcc
    16561 aaacgaaccg tgtggcccac cgcaatggcg gctgccagaa ccctctgttt tttctttgcg
    16621 gcagtacagg gcgggatgca tcccgccact cgtcatccct gtttgcacat ttgcgctgtc
    16681 g
//
LOCUS       DAORCQ010000046        16657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2170_length_16657_cov_5.462414, whole genome shotgun sequence.
ACCESSION   DAORCQ010000046 DAORCQ010000000
VERSION     DAORCQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_16657_cov_5.462414"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKI23_06470"
     CDS             complement(<1..833)
                     /locus_tag="PKI23_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNN36617.1"
                     /translation="MQQQTGSAAMSLATLLAMPGMAGGELPVTGLALDSRQVRPGDLF
                     LAVPGLHGDGRRHIDAALAAGAVAVLCEAEGFDLPTASAVPVVAIVALRQRLGELADR
                     FNHSPSTQLRVTGVTGTNGKSSVCHELAQLLGSQGDNCAVIGTLGNGFLGQLAPTRHT
                     TPDVVQLHGLLARLLAQGADSVAMEVSSHALDQGRVDGVRFHEAVFTNLSHDHLDYHG
                     DMQRYGAAKARLFVWPGLQRVVINLDDPFGRELLKRVPEGVEALSCSLERPEADLHAE
                     IR"
     gene            complement(833..2560)
                     /locus_tag="PKI23_06475"
     CDS             complement(833..2560)
                     /locus_tag="PKI23_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNN36618.1"
                     /translation="MKQAVALLEPASVWRLRLAAVLLSVAALAMMGRLFDLHVFDRDF
                     YQRHGDARAIRTETIEAHRGMIIDRNGHPLAVSTPLESICANPKSVLAAAERIPELAR
                     ALEIDPKELIAKLQAGAAARREFLYLRRQLPPDLASAILDLKVPGIYGRTEYRRYYPD
                     GEVTAHVLGFTNVDDHGQEGVELAFDGHLRGQDGRRKVLQDRRAEPIRELSRIRDPRP
                     GGELRLSLDARIQALAYRELKAAVQENGAAGGSVVMLDSTTGDVLAMVNQPSFNPNNR
                     IGLQPHNLRNRAIIDLVEPGSTVKPFTLAVALASGRYQPSTVIDTTPGYIQINGKVIR
                     DVHNYGVIDLSHVLSKSSNVGTIKVALTLEETAVRDLFDQVGLGRSTGSGFPGEAVGS
                     LPSGSMRHPLVRATLSFGYGLAVTPLQLAEAYSVLANDGVRRGIKILADSAVDPGVRV
                     IDSQVNRQIVAMLEQVVTPGGTGFKARVPGYRVAGKTGTVHKIGTGGYEASNYLSVFA
                     GFAPVEQPRVVAVIIIDNPTAGAYYGGAVAAPVFGKVMTGTLRLLDVPPDGLPEGGKG
                     LQVAKGGTP"
     gene            complement(2557..2829)
                     /gene="ftsL"
                     /locus_tag="PKI23_06480"
     CDS             complement(2557..2829)
                     /gene="ftsL"
                     /locus_tag="PKI23_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNN36619.1"
                     /translation="MSRVTNLILGALVVAVVASAIAVVQSTHLTRQAHGELRDLERQR
                     DQLQVEWSRLLLERGTLASHDRIKELAVSRLQMGAPDAANLLVVKP"
     gene            complement(2871..3821)
                     /gene="rsmH"
                     /locus_tag="PKI23_06485"
     CDS             complement(2871..3821)
                     /gene="rsmH"
                     /locus_tag="PKI23_06485"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNN36620.1"
                     /translation="MSREPVPIEPAHRPVMLEETMALWFSDGGGCYIDGTFGRGGHSG
                     ALLQRLSERGRLLALDRDPEAIAHGVQEFGGDARIELVHAAFDQLEALASSRGWRGQV
                     SGVLLDLGVSSPQLDEARRGFSFMRDGPLDMRMDPSRGESAAQWLARVDEAELERVLR
                     EYGEERFARRIAAAIVAAGRQTPLLGTVQLARVVAAAHPRWPKDRHPATQSFQAIRIA
                     INDELTLLQRALEQALEVLAIGGRLVVISFHSLEDRIVKRFFRHHARGDDLPAKLPVP
                     AALLKRRLRLLSGGQRAGEQELNSNPRARSAVLRAAEKLA"
     gene            complement(3821..4288)
                     /gene="mraZ"
                     /locus_tag="PKI23_06490"
     CDS             complement(3821..4288)
                     /gene="mraZ"
                     /locus_tag="PKI23_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNN36621.1"
                     /translation="MAFRGIAQVSLDPKGRIAIPARFRDQLLACGGAGLVVTIDTQQS
                     CLQLFPLPEWEVLQEKISALPSFNPATRRIQRLLLGHANDVEMDTAGRILLPPLLREY
                     ANLEKSKPVILLGQGNRFEIWAEDRWSVERDRWIEEERVQSGMVPDELRGFSI"
     gene            complement(4596..4952)
                     /gene="rnpB"
                     /locus_tag="PKI23_06495"
     ncRNA           complement(4596..4952)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI23_06495"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5142..5789)
                     /locus_tag="PKI23_06500"
     CDS             complement(5142..5789)
                     /locus_tag="PKI23_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNN36622.1"
                     /translation="MQSKSDIDALIDGFASFKQEYLARHHGQQKLKMQNLSTPRVMVV
                     GCCDSRFDPAIITNSDAGDIFVVRNVANLIPPCQPDKHYQETPAALEYGVCYLDVEEL
                     IVMGHGRCNGLRTLLHRLIDGSLASHPLAEWTEIAEQAAEQVLRAGFRSDLDWQVDQL
                     SRKALSLSLNNLERYPFIAARLNEGTLRLRGWYFDPDSAALEELDRASGQFRWLC"
     gene            6083..6727
                     /locus_tag="PKI23_06505"
     CDS             6083..6727
                     /locus_tag="PKI23_06505"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNN36623.1"
                     /translation="MRRTAWITLLLLTAPVTAFARAPDIAISGLFNGAALLTINGQPK
                     MLRVGEPAYAGVRLISADPHGAVVEVNGERRPLRLSEQIGDRFVPRERARATIVPDAR
                     QQYLTQGSINGLSVSMLVDTGATAIAMSETEARRLGIDFRYRGEESMVTTASHQVRAY
                     RVVLEQVTVGEITVHQVEAAILEGDLPEQILLGMSFLRHVELSERQGMLELVEK"
     gene            6817..7434
                     /gene="ribA"
                     /locus_tag="PKI23_06510"
     CDS             6817..7434
                     /gene="ribA"
                     /locus_tag="PKI23_06510"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNN36624.1"
                     /translation="MAVQHIASAALPTPWGEFEVHAFEDKATGKEHLALTLGSIGGGE
                     PVLIRLHSECLTGDALFSLRCDCGSQLQSALQQIAETGRGVLLYLRQEGRGIGLINKI
                     RAYHLQDGGADTVEANQRLGFEPDLRDYGMCGEMLAWLGVSRVRLLTNNPRKVEQLQR
                     QGVEVVERIPLETGQNPHNLRYLATKADKLGHLLDRSGMARCDEE"
     gene            complement(7455..9365)
                     /gene="dxs"
                     /locus_tag="PKI23_06515"
     CDS             complement(7455..9365)
                     /gene="dxs"
                     /locus_tag="PKI23_06515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNN36625.1"
                     /translation="MPVTFDEIPRQRPATPLLDQIDIPAQLRDLPEQQLPQLARELRE
                     YLLYTVGQTGGHFGAGLGVVELTIALHYVFDTPHDRLVWDVGHQAYPHKILTGRREAM
                     AGLRQQGGISGFPKRSESDYDTFGVGHSSTSIGAALGMALASRLKGEQRHHVAVIGDG
                     AMTAGMAFEALNHAAHVGNDLLVVLNDNDMSISRNVGGLSQYLARIWAGKTYNSVRQG
                     SKRLLSRIPPFWELARRTEEYMKGMVAPGVLFEELGFNYIGPLDGHDLPALVKTLSDM
                     KFLPGPRLLHVITRKGKGFAPAEQDPIGYHAISKLETRQGELLTVVNKGQKYSSIFGQ
                     WLCDMAEQEPRLIGITPAMAEGSDLVTFSQRYPQRYCDVGIAEQHAVTLAAGMACEGA
                     KPVVAIYSTFLQRAYDQLIHDVAIQNLDVLFALDRAGLVGEDGATHAGSFDLSYLRAI
                     PNLLVMAPSDENETRQMLYTGFIHPGPAAVRYPRGTGPGAEVVEVMSALEIGRALVRR
                     QGREVALLAFGSMVRPALAAAERLDATVVDMRFVKPIDEALVLRLAESHQLLVTIEEN
                     VVMGGAGSAVNECLAAHGVLMAPLNLGLPDRFIDHGKTAALLAECGLDAEGICRSVEA
                     RLPRRESAAGLG"
     gene            complement(9462..10361)
                     /locus_tag="PKI23_06520"
     CDS             complement(9462..10361)
                     /locus_tag="PKI23_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNN36626.1"
                     /translation="MSAAFDQWADPHRRRLEALIDHHLPAADPAAARLFEAMRHAMQG
                     GGKRIRPLLVHAACESCGGTAEVAELPALAVELIHGYSLVHDDLPAMDDDDLRRGRPT
                     CHIAFDEASAILAGDALQSLAFELLATASQPSVELRMAMVLTLARAAGGGGMVGGQAL
                     DLAVEARHDPPLTLAELESLHARKTGALLRASVRLGALAAGEQDALRLTALDRHAAAL
                     GLAFQIRDDILDVEGETALLGKRQGADAARGKLTYPRLLGMDEAKARAEALHQQALAA
                     LEPLGSRAWPLRALCEQLQQRSH"
     gene            complement(10358..10588)
                     /locus_tag="PKI23_06525"
     CDS             complement(10358..10588)
                     /locus_tag="PKI23_06525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017129933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNN36627.1"
                     /translation="MTTKRKKSDFDQSLTRLESLVTTLERGDIPIEEALDLFEQGVKL
                     ARECQGSLRDAQQKVEILLADGETRQPFEPAE"
     gene            complement(10645..13332)
                     /locus_tag="PKI23_06530"
     CDS             complement(10645..13332)
                     /locus_tag="PKI23_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNN36628.1"
                     /translation="MHLYALPIDEALARLNTTAEGLSEAEAARRLASFGVNEMEASAR
                     EPLWRGAARQVTHFFALILWLAAALAFVAEHYQPGQGMATLGAAILGVIVINGAFSFW
                     QEYRAERALDALQRLLPHAVRVQRAGVVRLAPARELVPGDVILLGAGDQIPADCRVLE
                     AYAARVNTATVTGEALPMTVSAEPSTAAEALHARNLLLAGTALVAGEARAVVFATGMH
                     SEFGRIAQLTQATSKGLSPLQQEITRLSRWVAALAVALGVTFFVIGQALGLPLWGNVL
                     FAIGIIVANVPEGLLPTVTLALAMGAQRMARRRALIRHLPSVETLGSATVICTDKTGT
                     LTENRLRAQRLFVDGNSVAADEWTAPPNAPLVAVAAHCHALAADAAVDVAALDPLEAA
                     LIDFARERGGAPAVETLQVLPFDSERRRMAVLAQRDGERALYAKGALEALLPLCSAVR
                     QGDAIVPLTDAARAAVLAAEAQLAADGLRVIALASRPVSPDTAPVTALGATPEQLERD
                     LVLAGLVALHDPPRPEVPAAIARCRSAGIRTIMVTGDHPRTALVVARQIGLANSEHST
                     VVSGERLARLTDTELQLLLDGPEVLFARVSAEQKRRIVAVLKRRGEIVAVTGDGVNDA
                     PALREAHIGIAMGVAGTDVAKAAADMVLLDDNFATIVAAVEEGRGVFDNLRKFLTYIL
                     TSNIPEVVPYLAFALLRIPLPLTVIQILAVDLGTDMLPALALGAERPAAQVMQRPPRR
                     ASERLIDGALIARAYLFLGLLEAGAAMAAYFFVLHAGGWQYGQTLAPTDPLYRAATGA
                     CLAAIVLMQVANVWLCRSARGALFAGNGRPSPLLYWGIGFELLLLAAILYTPFGNALF
                     GTAPLAMEVWWFMLPFPLAMLALEEARKAVLRWWSQVAG"
     gene            complement(13340..13963)
                     /locus_tag="PKI23_06535"
     CDS             complement(13340..13963)
                     /locus_tag="PKI23_06535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNN36629.1"
                     /translation="MAFYEEKILPGLLDLACSTRDVMALRAKIVPLARGRVLEVGIGS
                     GHNLALYDSGQVELVWGLEPSAGMRGRAQRNLARSAVEVRWLDLPGEQIPLEDNSVDT
                     VLLTFTLCTIPDWRLALQQMARVLKPGGKLLFCEHGRAPDVAVSRWQDRLNPLWKRLF
                     GGCHLNRPIADCLAEGGFAIEQLDSNYAEGMPRFAGYISMGQAVKGQ"
     gene            14190..>16657
                     /gene="mgtA"
                     /locus_tag="PKI23_06540"
     CDS             14190..>16657
                     /gene="mgtA"
                     /locus_tag="PKI23_06540"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNN36630.1"
                     /translation="MADEALDAYWSLPTPKLHAALHSGAAGLTSSEAARRLAEHGPNR
                     LPDGPALAPLALLLKQFTDPLVLILLFAGLLSLYLHEWLDAAIVLGIVLGSGGLGFVQ
                     EYRASAAVAALREQVAARVTVLRDRAPVNLPAEAVVPGDVVQLAAGSLIPADGVLLEA
                     KDFFVTEAVLTGETFPVEKTPGTAPPQAGLAERHGCVFMGTSVRSGTARALIVETGAG
                     TAFGAIAHRLRRRAPPTEFELGIRRFGALLTQVMVALTLAVFAINVHFERPVVEALLF
                     SVALAVGLSPELLPAIMTLTLSHGARAMARQGVIVRRLAAIENLGSMDVLCTDKTGTL
                     TVGVIKLDGALGGDGQPSARVAQLTYLNAHFETGIDNPLDAAIEAAGVPAGLNPAAWR
                     KLDEIPYDFLRKRLSIVAAGPDGAPTLLTKGAFASVLAVCERLRLGEAEVALDDAQRA
                     ALEQRFAAWSEQGYRVLGVASRRLDERPAYHASDETGLCFEGFLLFFDPPKPGIERTL
                     ADLGRLGVAVKIITGDNRLVAAHLAREVGIAEPVVLTGAQMDDMRDEALWQRVVDTDL
                     FAEVDPNQKERLILALKRSGHVVGYLGDGINDAPALHAADAGISVDQAVDVAKQAADF
                     VLLQHDLDVLCRGIEFGRSTFANTLKYIYTTTSANFGNMISMAAASLFLPFLPLLPKQ
                     ILLNNFLSDLPAMGIATDRVDAEAVQTPHRWNVAAIRRFMLTFGLISSLFDGLTFALL
                     LWLTDGAPAHFRTGWFVESLLTELWVLLLVRTARPAWRSRPGTLLWSLTLALTGVAIA
                     LPYLPASHALFEFTPLPANVLAAVL"
BASE COUNT         2937 a         5799 c         5234 g         2687 t
ORIGIN      
        1 cggatttcgg catgcaggtc ggcctcgggc cgctccaggc tgcaggagag cgcctcgaca
       61 ccctccggca cccgcttcag gagttcacga ccgaacgggt catcgaggtt gatgaccacc
      121 cgctgcagcc ccggccagac gaacagccgc gccttggcgg cgccgtagcg ctgcatgtcg
      181 ccgtgatagt cgagatggtc gtggctcagg ttggtgaaga ccgcctcatg gaagcgcacg
      241 ccatcgaccc gcccctgatc gagcgcatgt gaagagacct ccatcgccac cgaatcggcc
      301 ccctgcgcca gcagtcgggc cagcaggcca tgcagttgca cgacatcggg ggtggtgtga
      361 cgggtcggcg ccaattggcc aagaaagccg ttgccaaggg tgccgatcac cgcgcagttg
      421 tcgccctggc tgcccagcaa ctgcgccagc tcatgacaga ccgagctctt gccattggtg
      481 ccggtgaccc cggtgacccg cagctgcgtc gatggtgaat ggttgaagcg gtcggcaagc
      541 tcacccaacc gctgccgcag cgccacaatg gccaccaccg gcacggcact ggctgtcggc
      601 agatcaaagc cctccgcctc gcaaagcacc gccaccgccc cggcggccag cgccgcatcg
      661 atatgacgcc gaccatcgcc atgcagtccc ggcaccgcca gaaacagatc tcccggccgc
      721 acctgccgac tgtcgagcgc aaggccggtc accggcaact caccgccggc catccccggc
      781 atggccagca gcgtggcgag actcattgcg gcactgcccg tctgctgctg catcatggcg
      841 tgccaccctt ggccacctgc agccccttgc cgccctcggg cagtccatcg ggcggcacat
      901 ccagcagccg cagggtaccg gtcatgacct tgccgaacac cggcgccgcc accgcaccac
      961 cgtagtaggc acccgcagtg gggttgtcga tgatgatgac ggccaccacc cgcggctgct
     1021 cgaccggcgc gaagccagca aagaccgaca ggtagttgct cgcctcataa ccgccagtgc
     1081 cgatcttgtg gaccgtgccg gtcttgccgg ccacccggta gcccggcacc cgcgccttga
     1141 agccggtgcc gcccggggtc accacctgct cgagcatcgc gacgatctga cggttgacct
     1201 gcgaatcgat gaccctgacc cccggatcca ccgcgctgtc ggccagaatc ttgatcccgc
     1261 gccgcacacc atcgttggcc agcaccgaat aggcttcggc cagttgcagc ggggtcacgg
     1321 ccagcccata gccaaacgag agcgtggccc gcaccagtgg atggcgcatc gagccggatg
     1381 gcagcgatcc caccgcctca ccaggaaagc cactgccggt ggaacgcccg agtcccacct
     1441 gatcgaacag gtcgcgcacc gccgtctctt ccagcgtcag cgccacctta atggtgccga
     1501 cattgctcga cttggagagc acatggctca gatcgatcac gccgtagttg tggacgtcac
     1561 gaatcacctt gccattgatc tggatgtatc ccggcgtggt gtcgatgacc gtgctcggct
     1621 gataacggcc gctcgccagc gccaccgcca acgtgaaggg cttgaccgtg gagcccggct
     1681 cgaccagatc gatgatcgcg cggttgcgca gattgtgcgg ctggagtcca atgcggttgt
     1741 tggggttgaa ggagggctga ttgaccatcg ccagcacatc accggtggtc gaatcgagca
     1801 tcaccaccga accaccggcg gcgccattct cctgcaccgc cgccttcagt tcgcggtagg
     1861 ccagcgcctg gatgcgggca tcgagactga gcctgagctc tccgcccgga cgcggatccc
     1921 tgatgcgact cagctcacga atcggctcgg cacgccgatc ctgcagcacc ttgcgccgtc
     1981 catcctgacc gcgcagatgg ccatcgaagg ccagctccac cccctcctga ccgtggtcgt
     2041 cgacattggt gaaacccagc acatgggccg tcacctcgcc atcggggtag taacgtcgat
     2101 attcggtccg accgtagatg cccggcacct tcaaatcgag aatcgcactg gccagatcgg
     2161 gcggaagctg acgacgcagg tagagaaatt cccgacgcgc ggcggccccc gcctgcagct
     2221 tggcgatcag ctctttcggg tcgatttcga gcgcgcgtgc cagctccgga atccgctcgg
     2281 cagcggccaa aacggatttg ggattggcgc agatcgactc cagcggcgta ctcactgcca
     2341 gcggatgacc attgcggtcg atgatcatgc cgcggtgcgc ctcgatcgtc tcggtgcgga
     2401 tggcccgcgc gtcgccatgc cgctgataga agtcgcggtc gaaaacatgc aggtcgaaca
     2461 accgccccat catcgccagc gccgccaccg acaacaggac ggcggccagc cgcagccgcc
     2521 agaccgaagc cggttccagc agtgcgaccg cctgtttcat ggcttcacca ccagcagatt
     2581 ggcggcatcc ggcgccccca tctgcaaccg gctgacggcc agctccttga tccgatcgtg
     2641 tgaagccagg gtgccgcgct cgagcagcag gcggctccac tcgacctgca actggtcacg
     2701 ctgccgctcc agatcccgca gctcgccgtg cgcctgccgg gtcagatggg tgctctgcac
     2761 cacggcgatc gccgaagcca ccaccgccac aaccagcgct cccaatatca ggttcgtcac
     2821 tcggctcatg cccgttttcc gttctgtttt catcgggttc cgccttcaac tcaggccagc
     2881 ttctcggccg cacgcagcac cgcactgcgc gcccgcggat tgctgttcag ctcctgctcg
     2941 cctgcgcgct gaccacccga cagcagccgc aggcggcgct tgagcagagc ggccggcacc
     3001 ggcaacttgg ccggcagatc gtcaccgcgc gcgtggtgac gaaaaaaccg cttgacgatg
     3061 cgatcttcca gcgagtggaa gctgatcacc accagccgcc ctccgatcgc cagcacttcg
     3121 agcgcctgct ccagtgcacg ctgcaacagc gtcaactcat cgttgatggc gatgcggatc
     3181 gcctgaaaac tctgggtggc tggatgacga tccttgggcc agcgagggtg agccgccgcc
     3241 accaccctgg ccagctgcac cgtgccaagc agcggtgtct gccgcccggc cgcgacaatc
     3301 gccgccgcga tgcggcgagc gaagcgctct tcaccatact cgcgcagcac ccgttccagc
     3361 tccgcctcat cgacccgcgc cagccactgg gctgcacttt cgccccgact gggatccatg
     3421 cgcatgtcga gcggcccatc gcgcatgaaa ctgaaaccgc gccgcgcctc gtcgagctgc
     3481 ggcgatgaga cccccagatc gagcagcacg ccactcacct gaccacgcca gccacggctg
     3541 ctggccagtg cttccaactg gtcgaacgcg gcgtggacca gctcgatgcg cgcatcgccg
     3601 ccgaattcct gcacgccatg ggcgatcgct tccggatcac ggtcgagcgc cagcagtcgt
     3661 ccccgctcgc tcaaccgctg caacagcgcc ccgctgtgcc cgccacgacc gaaggtgcca
     3721 tcgatgtagc aaccaccgcc atcgctgaac cagagcgcca tcgtttcctc cagcatcacc
     3781 ggtctgtgcg ccggctcgat cggaacaggc tcccgcgaca tcagatcgaa aacccgcgca
     3841 gctcatccgg caccatgcca ctctggacgc gctcctcttc gatccagcga tcccgctcga
     3901 cactccagcg atcctcggcc cagatctcga aacggttgcc ctgcccaagc aggatcaccg
     3961 gcttgctctt ttccagattg gcgtattcgc gcagcagcgg cggcaacaga atgcgccctg
     4021 ccgtgtccat ctcgacatcg ttggcatgac ccagcaacag tcgctgaatg cgtcgggtgg
     4081 ccggattgaa gctcggcagc gcgctgattt tttcctgaag cacctcccat tccgggagcg
     4141 gaaaaagctg caaacaactc tgctgggtat cgatggtcac caccaatccg gcaccaccgc
     4201 aggccaaaag ctggtcacga aaccgggccg ggatcgcaat gcgacccttg ggatcaagac
     4261 tgacctgcgc aatgccgcga aatgccatga agtttccacc cagcgggagg gcatttcagc
     4321 cctttcacgc cacataacga cacttttcac caccacgcac actatatgga gcgcaaaatg
     4381 ggcttgtcaa ggaaaatcac ggcctctcac agcagaaacg ctgtcattaa aattcttttt
     4441 taatcatata gataaaatta ttatcaagca cttgccaacc aaagcaaaac agtctcgcaa
     4501 gtgtggagca agactggcgc gaaaccgacc cgcgcagaca gattgcagtc gccgtgcaac
     4561 ggttcaatcg aagcagagat gagaagggga agggtgcatg gagccggccg ataagccggg
     4621 ttctgtcgag gacaatcatt catctgggcc gcgaatcact ccgcggctct agcgacctac
     4681 ccgaatccgg tgcgggccgc accgatggat tcctatttgg tcttgctccg ggcggggttt
     4741 gccatgccac gcgtgttgcc acccgtgcgg tgcgctctta ccgcaccatt tcacccttac
     4801 cacaccgacg atgtgtcgcc agcgggcggt atcttttctg ttgcactttc cgtgggctcg
     4861 cgccccccag gcgttacctg gcgccctgcc ctgtggagcc cggactttcc tccccacctg
     4921 caaccaggca gagcgattgt ctggccaact ccatgccgca ccagcttaac caactcggca
     4981 caccggcgac aagcctttcg gcccagacaa ggtcactgtt ttttcattct ggcatcaaaa
     5041 atgctgacag actggaggca gacccaatga aaaccccggt gggtgtgcac cggggttttc
     5101 ttttttgtgt gacgcttcgc cagcagacgg catgaaacag ctcagcacag ccatcggaat
     5161 tgaccactgg ctctgtccag ctcttcgagg gcggctgagt cgggatcaaa ataccagcca
     5221 cgcaatcgca atgtcccctc gttcaggcgc gcggcgatga agggatagcg ctcgaggttg
     5281 ttcagcgaca acgacagcgc cttgcggctc aactggtcga cctgccaatc gagatcgctg
     5341 cggaaccccg cgcgcaacac ctgttcggca gcctgctcgg cgatttcggt ccactccgcc
     5401 agcggatggc tggccaaaga gccatcgatc agacggtgca gcagcgtgcg caggccgttg
     5461 cagcgaccat gccccatgac gatcagctct tcgacatcga ggtaacagac gccatattcg
     5521 agcgctgccg gggtctcctg gtagtgcttg tcgggctggc agggcggaat caggttggca
     5581 acgttgcgca ccacgaagat gtcgccggcg tcggagttgg tgatgatggc cggatcgaac
     5641 ctggagtcac agcaacccac gaccatcacc cttggcgtcg acagattctg catcttcagc
     5701 ttctgctgcc catggtggcg cgccagatac tcctgcttga agctggcaaa accgtcgatc
     5761 agcgcgtcga tgtcagactt cgactgcatc gttccatcac cgcgcagggc aagatggcga
     5821 gagtgggaca gcatgggaat caacctccat gaacacaggg catttctcaa aaaatactca
     5881 agacaagtta catgccaatg atgcatggag gcccattccg gccgatggga agccccgttg
     5941 cggtttttcg acaccatcct gcgccagatt ggggcgacag cgccgcgcgg aggccaagtc
     6001 gagtgcatcc gaacgggtga ccgccattca catcggcagc gagcgtgcta aggtttattc
     6061 aggcccaatc tggatgaatc ccatgcgtcg tacagcctgg atcacactgc tgcttctgac
     6121 cgcaccagtc accgccttcg ccagggcgcc agacattgcc atctccggac tgttcaatgg
     6181 cgcggcactg ctgaccatca atggtcaacc gaagatgttg cgggtgggcg agccggccta
     6241 cgccggagtc cgcctgattt cggccgatcc gcatggggcc gtggtcgagg tgaatggcga
     6301 gcgcaggccg ttgcggctct cggagcagat cggcgaccgc tttgtgccaa gagagcgcgc
     6361 acgggccacc atcgtgccgg atgcccggca gcaatacctg acccagggca gcatcaatgg
     6421 cctgagcgtc tccatgctgg tcgacaccgg cgccacggcc atcgccatga gcgaaaccga
     6481 ggcacgtcgg cttggcatcg actttcgcta ccggggcgaa gagtccatgg tcaccacggc
     6541 cagccaccag gtgagagcct accgggtcgt gctggagcag gtgacggtcg gtgagatcac
     6601 cgtacatcag gtcgaggcgg ccattctcga gggtgatctg cccgagcaga tcctgctggg
     6661 gatgagcttt ttgcggcacg tcgagctctc cgaacgtcag ggcatgctgg agttggtcga
     6721 aaaataacct gaaacgccca gccggctcag cagggcattc gcctcggctg cgtgttcgtt
     6781 gctaaaatcg ccggcttgct gttcaagagg tggcgtgtgg cagtccaaca tatcgcatcg
     6841 gcggcactac ccactccctg gggggagttc gaggtgcatg ccttcgaaga caaagcgacc
     6901 ggcaaggagc atctggcgct gacactgggg tcgatcggcg gcggtgagcc ggtgctgatc
     6961 cggctgcatt cggagtgcct gaccggcgac gcgctgttca gcctgcgttg cgactgtggc
     7021 tcacagctgc aaagcgcgct gcagcagatt gccgagaccg gccgcggcgt gctgctctac
     7081 ctgcgccagg aaggacgcgg cattggtctg atcaacaaga tacgggccta tcacctgcag
     7141 gatggcggcg ccgacaccgt cgaggccaac cagcggctcg gcttcgagcc cgatctgcgt
     7201 gactatggca tgtgcggtga aatgctggca tggctcggcg tcagccgggt gcggctgctg
     7261 accaacaatc cgcgcaaggt cgaacaactg caacggcaag gggtcgaggt ggtggagcga
     7321 attccgctgg agaccggcca gaacccgcac aatctgcgct atctggccac caaggccgac
     7381 aagctcggcc acctgctcga ccgcagcggc atggcccgct gcgacgagga gtgatctcgc
     7441 ccgtccacac ggtctcaccc cagccctgcc gcgctctccc gacgcggcag gcgcgcctcg
     7501 accgaccgac agatgccctc ggcatccagc ccgcattcgg ccagcagcgc agccgttttg
     7561 ccatggtcga tgaagcggtc cggcagaccg agattgagcg gcgccatcag cacgccgtgt
     7621 gcggccaggc actcgttcac cgctgaacca gcgcccccca tcaccacatt ctcttcgatg
     7681 gtcaccagca gctggtggct ttcggccagc cgcagcacca gcgcttcatc gatcggtttg
     7741 acgaaacgca tgtcaaccac ggtggcgtcg agccgttcgg cggcggcaag cgccgggcgc
     7801 accatcgatc caaaggccag cagcgccacc tcgcgtcctt ggcgccgcac cagagcgcgg
     7861 ccgatctcca gcgcgctcat cacctcgacc acctcggccc ccggccccgt gccgcgcggg
     7921 tagcgcacgg ccgcgggtcc tggatggatg aagccggtgt agagcatctg ccgcgtctcg
     7981 ttctcgtccg atggcgccat caccagcaga ttcggaatcg cgcgcaggta gctcaggtcg
     8041 aagctgccgg catgggtggc gccatcctcg cccaccagac cggcgcggtc gagcgcaaac
     8101 agcacgtcga gattctgaat ggcgacatcg tggatcaact ggtcataggc ccgctgcaaa
     8161 aaggtggagt agatcgccac caccggcttg gccccttcgc aggccatgcc ggcagccaag
     8221 gtcactgcat gctgctcggc aatgccgaca tcacagtaac gctgcggata gcgctgcgaa
     8281 aaggtcacca gatcggagcc ctcggccatc gccggggtga tgccgatcag ccgcggctcc
     8341 tgctccgcca tgtcgcacag ccactggcca aagatgctgg agtacttctg tcccttgttg
     8401 acgacagtca aaagctcgcc ctgccgggtc tcgagcttgg agatggcgtg gtaaccgatc
     8461 ggatcctgct cggccggtgc gaagcccttg cccttgcggg tgatcacatg cagcagtcgc
     8521 ggacccggca gaaatttcat gtcgctcagc gtcttgacca gcgccggcag gtcgtggcca
     8581 tcgagcggac cgatgtagtt gaagccgagc tcctcgaaca gcacaccggg ggccaccatc
     8641 cccttcatgt actcctcggt gcggcgcgcc agctcccaga atggcggaat gcggctcagc
     8701 agccgcttgc tgccctggcg caccgagttg taggtcttgc cggcccagat gcgcgccaga
     8761 tattgcgata gcccgccgac attgcgcgaa atcgacatgt cgttgtcgtt cagcaccacc
     8821 agcaggtcat tgccgacgtg ggctgcatgg ttcagtgcct cgaaggccat gcccgcggtc
     8881 atcgccccat cgccgatcac cgccacatgg tggcgctgct cgcccttgag cctgctggcc
     8941 agcgccatgc cgagtgcggc accgatcgag gtgctggagt ggccgacacc aaaggtgtcg
     9001 taatcactct cgctccgctt cggaaagccg gagatacccc cctgctgacg cagcccggcc
     9061 atggcctcgc gccgaccggt cagaatcttg tgcggatagg cctgatggcc gacatcccat
     9121 accagccggt catgcggcgt gtcgaacaca tagtgcagcg cgatggtcag ctcgaccacg
     9181 cccagaccgg cgccgaagtg gccaccggtc tggccgacgg tgtagagcag atactcacgc
     9241 agttcacggg cgagctgcgg caactgctgc tccggcaggt cacgcagctg cgccgggatg
     9301 tcgatctgat cgagcagcgg cgtggctggc cgctgtctgg gaatttcgtc aaaagtgacc
     9361 ggcatggttc agcccgacaa aacgcggcga caacagaaat tgatttgatt gcaaatcata
     9421 ccaccgtcgg ccgctttcac tctcatgccg accggcgccg ttcagtggct gcgctgctgc
     9481 aactgctcgc acagtgcgcg cagtggccag gcccgactgc cgaggggttc gagcgccgcc
     9541 agcgcctgct gatgcagcgc ttcggcccgc gccttggcct catccatgcc cagcagccgc
     9601 ggataggtca gcttgccacg cgcggcatcg gcaccctgcc gcttgccgag cagcgccgtc
     9661 tcaccctcga catcgagaat gtcatcccgg atctgaaagg ccagccccaa ggccgcggca
     9721 tgacgatcga gcgccgtgag ccgcagcgca tcctgctccc ctgcggccag tgcacccagt
     9781 ctgacactgg cgcgcagcag tgcaccggtc ttgcgtgcat gcagcgactc cagttcggcc
     9841 agcgtcagcg gcgggtcatg gcgcgcctca acggccagat cgagcgcctg tccgcccacc
     9901 attccgccac caccggccgc ccgcgccagc gtcagcacca tcgccatgcg cagttcgacg
     9961 ctgggttggc tggctgtcgc caacagttcg aaggccaggc tctgcaaggc atcgccggcc
    10021 agaatcgcac tggcctcgtc aaaggcgatg tggcaggtgg gacggccacg gcgcaggtcg
    10081 tcatcatcca tcgccggcag gtcatcatgc accagcgaat agccatggat cagctcgacc
    10141 gccagggccg gcagctccgc gacctctgcc gtgccaccac agctttcgca ggccgcatgg
    10201 accagcagcg gacgaatccg cttgccaccg ccctgcatcg catggcgcat cgcctcgaac
    10261 aggcgggccg ccgccggatc ggccgcaggc aggtggtgat cgatcagcgc ttcgagccgc
    10321 cgccgatgtg ggtcggccca ctgatcgaag gctgcactca ttccgccggc tcgaagggtt
    10381 ggcgggtttc gccatcggcg agcaggatct ccaccttctg ctgggcatcc cgcaggctgc
    10441 cctggcattc tcgtgccagc ttgacgccct gctcgaacag gtcgagcgcc tcctcgatcg
    10501 ggatgtcacc tcgttcgagc gtggtgacca gactctccag ccgggtgagt gattgatcga
    10561 agtcgctctt tttgcgcttg gtggtcatgg gggacagatg catcggttca aatctgcatg
    10621 gtagcgcagt cgaggtcgat ctccctagcc cgccacctgg ctccaccacc gcagcaccgc
    10681 cttgcgcgcc tcttccagcg ccagcatcgc aagcggaaac ggcagcatga accaccacac
    10741 ctccatcgcc agcggcgcgg tgccgaacag cgcattgccg aagggcgtat agagaatcgc
    10801 cgcaagcagc agcagttcga agccgatgcc ccaatacagc agcgggctcg gccggccgtt
    10861 gccggcgaac agcgcgccgc gcgcggagcg gcacagccag acattcgcca cctgcatcag
    10921 cacgatggcg gccagacacg cgccggtggc ggcgcggtag agcgggtcgg tgggcgccag
    10981 cgtctgcccg tactgccagc cgcccgcgtg cagcacgaag aagtaggccg ccatggcggc
    11041 gccggcttcg agcagaccca ggaacagata ggcgcgtgcg atcagcgcgc cgtcgatcag
    11101 ccgctcgctg gcgcggcgcg gcgggcgctg catcacctgc gcggcgggcc gttctgcgcc
    11161 gagcgccagc gccggcagca tgtcggtgcc cagatccacc gccaggatct ggatcaccgt
    11221 cagcggcagc gggatgcgca gcagcgcgaa cgcgaggtag ggcaccacct cggggatgtt
    11281 cgaggtgagg atgtaagtca ggaacttgcg caggttgtcg aacacgccgc gcccttcctc
    11341 caccgcggcg acgatggtgg cgaagttgtc gtcgagcagc accatgtcgg cggcggcctt
    11401 ggcgacatcg gtgccggcca cgcccatggc gatgccgatg tgcgcctcgc gcagcgccgg
    11461 ggcgtcgttc acgccgtcgc cggtgacggc gacgatctcg ccgcggcgct tgagcaccgc
    11521 gacgatgcgc cgcttctgtt cggcgctgac gcgggcgaac agcacctccg gcccgtcgag
    11581 cagcagttgc agctcggtgt cggtcaggcg cgccaggcgc tcgccgctga ccaccgtcga
    11641 gtgctcgctg ttggcaagac cgatctgccg cgccaccacc agcgccgtgc gcggatggtc
    11701 gccggtgacc atgatggtgc ggatgccggc actgcggcag cgggcgatgg cggccggcac
    11761 ttcaggccgc ggcgggtcat gcagggcgac gaggcccgcc agcaccaggt cgcgttcgag
    11821 ctgttcgggc gtggcgccaa gggcggtgac gggcgcggtg tcgggcgaca cggggcggct
    11881 cgccagcgcg atcacccgca gtccgtccgc cgcgagctgc gcctcggcgg ccagcaccgc
    11941 cgcgcgcgcg gcatcggtga gcggcacgat cgcgtcccct tgccgcacgg cgctgcacag
    12001 cggcagcagc gcttcgagcg cgcccttggc gtagagcgcc cgctcgccgt cgcgctgcgc
    12061 cagcaccgcc atgcgccggc gctcggaatc gaacggcagc acctgcaggg tctccaccgc
    12121 cggcgcgccg ccgcgctcgc gcgcaaagtc gatcagcgcc gcctccagcg ggtcgagcgc
    12181 tgcgacatcc accgccgcgt cggccgccag tgcgtggcag tgggcggcga ccgccaccag
    12241 cggtgcattc ggcggcgccg tccactcatc ggccgcgaca ctgttgccat ccacaaacag
    12301 ccgctgcgcc cgcaggcggt tctcggtcag cgtgccggtc ttgtcggtgc agatcacggt
    12361 ggccgagccc agcgtctcca ccgacggcag gtggcggatc aatgcccgac gccgcgccat
    12421 gcgctgcgcg cccatggcga gcgccagcgt caccgtcggc agcagcccct ccggcacgtt
    12481 ggcgacgatg atgccgatgg cgaacagcac gttgccccac agcggcaacc cgagcgcctg
    12541 gccgatgacg aagaaggtca cgccgagcgc cacggcgagc gccgccaccc agcgcgacag
    12601 gcgcgtgatc tcctgctgca acggcgacag ccccttactc gtggcctgcg tgagctgcgc
    12661 gatgcgcccg aactcgctgt gcatgccggt ggcgaacacc accgcgcgcg cctcgccggc
    12721 caccagcgcc gtgccggcca gcagcaggtt gcgcgcgtgg agtgcctcgg cggccgtgga
    12781 gggctcggcc gacaccgtca tcggcagcgc ctcgccggtc accgtggcgg tgttgacgcg
    12841 cgctgcgtag gcctccagca cccggcagtc ggctggtatc tgatcgccgg cgccgagcag
    12901 gatcacatcc cccggcacca gctcgcgcgc tggggccagg cgcaccacgc ccgcgcgctg
    12961 cacccgcacc gcgtgcggca gcaggcgctg cagggcatcg agtgcccgct cggcgcggta
    13021 ctcctgccag aacgagaacg cgccgttgat gacgatcacg cccaggatcg ccgcgcccag
    13081 cgtggccatg ccctgccccg gctggtaatg ctctgcaacg aacgccagcg ccgccgccag
    13141 ccacaggatc agcgcgaaga aatgcgtcac ctgccgcgcc gcgccgcgcc acagcggctc
    13201 ccgcgccgag gcttccattt cgttgacacc gaagctggcg agacgccgcg cggcctcagc
    13261 ctcgctcaga ccctccgctg tcgtattgag gcgggccagc gcttcatcga tcggcagcgc
    13321 atacaggtgc atcgccaggt cactgaccct tgaccgcctg tcccatcgaa atgtagccag
    13381 cgaagcgggg catgccctcg gcatagttgc tgtcgagctg ctcgatggca aagccgccct
    13441 cggccagaca gtcagcgatc gggcggttga ggtggcagcc gccgaagaga cgcttccaaa
    13501 gcggattcaa tcgatcctgc catctggaga ctgccacatc gggtgcccgg ccatgctcgc
    13561 agaacagcaa tttaccgccc ggtttcaaca cccgcgccat ctgctgcagc gccaggcgcc
    13621 aatccggaat ggtgcaaagg gtaaaggtca acagcacggt gtcgacgctg ttgtcttcga
    13681 gcggaatctg ctcgcccggc agatcaagcc agcgcacctc gactgcggag cgggccaggt
    13741 tgcgctgagc ccgaccgcgc atcccggccg agggttccag cccccagacc aactcgacct
    13801 gaccggagtc atacagagcc agattgtgcc cggaaccgat gccaacctcg agcacccggc
    13861 ctcgcgccag cggcacgatc ttcgctctca gcgccatcac atcgcgtgtc gagcaggcca
    13921 gatcgagcag acccggcaag atcttctctt cataaaaagc cataaacaca cctcggaacc
    13981 ggtcggacag taaaggagac actcccgaaa tccatgagca gcaacccacc ggcacagtaa
    14041 aagagcggca tcactttctc aagcatccgt atcgacgatg ccgctcgctt ccgcaacagc
    14101 aactcgaatc ggcaacagac cacgacagtg gaaaaatcga tacacttggc ccattgaccg
    14161 gttatcaatg ccatcaatgg gatcgtctca tggccgatga agcgctggat gcctactggt
    14221 ccctgccaac accgaaactc catgccgcac tgcacagtgg cgcagccggt ctgaccagca
    14281 gcgaagccgc ccgccgcctt gccgagcacg gcccgaaccg cctgccggac ggcccggcgc
    14341 tggcgccgct ggcgctgctg ctgaagcagt tcaccgatcc gctggtgctg atcctgctgt
    14401 tcgccggcct gctgtcgctc tacctgcacg agtggctcga cgccgccatc gtgctcggca
    14461 tcgtgctcgg cagcggcggg ctcggcttcg tgcaggagta ccgcgcctcg gcggcggtgg
    14521 cggcgctgcg cgaacaggtc gcggcgcggg tgacggtgct gcgcgaccgc gcgccggtga
    14581 acctgccggc cgaggcagtg gtgccgggcg acgtggtgca gctggccgcc ggcagcctga
    14641 tcccggccga cggcgtgctg ctggaggcga aggacttctt cgtcaccgag gcggtgctga
    14701 ccggcgagac cttcccggtc gagaaaaccc ccggcacggc gccgccgcag gcgggccttg
    14761 ccgagcgcca cggctgcgta ttcatgggca cctcggtgcg cagcggcacg gcccgggcgt
    14821 tgatcgtcga gaccggtgcc ggcacggcgt tcggcgccat cgcccaccgc ctgcgccggc
    14881 gcgcgccgcc gaccgagttc gagcttggca tccgccgctt cggcgcgctg ctgacgcagg
    14941 tgatggtggc gctgacgctg gcggtgtttg ccatcaacgt ccacttcgag cggccggtgg
    15001 tggaggcgct gctgttctcg gtggcgctgg cggtggggct gtcgccggag ctgctgccgg
    15061 ccatcatgac cctcacgctg tcgcacgggg cgcgggccat ggcccgtcag ggcgtgatcg
    15121 tgcgccggct cgccgccatc gagaacctcg gcagcatgga tgtgctgtgc accgacaaga
    15181 ccggcacgct gacggtgggg gtgatcaagc tcgacggcgc actgggtggc gatgggcagc
    15241 cctcggcgcg ggtggcgcag ctcacctacc tcaacgccca cttcgagacc ggcatcgaca
    15301 acccgctcga cgccgccatc gaggcggccg gcgtgccggc cggcttgaac ccggccgcct
    15361 ggcgcaagct cgacgaaatc ccctacgact ttctgcgcaa gcggctgtcc atcgtcgccg
    15421 ccgggccgga cggcgcgccg acgctgctga ccaagggcgc cttcgccagc gtgctggcgg
    15481 tgtgcgagcg cctgcgcctg ggcgaggccg aagtcgccct cgacgacgcg caacgggccg
    15541 ccctcgaaca gcgctttgcc gcgtggagcg aacagggtta ccgcgtgctc ggggtggcca
    15601 gccgcaggct cgacgagcgg cccgcctacc acgccagcga cgagaccggc ctctgcttcg
    15661 agggctttct gctgttcttc gacccgccca agcccggcat cgagcgcacg ctggccgacc
    15721 tcgggcgtct gggcgtggcg gtcaagatca tcaccggcga caaccgcctg gtggcggcgc
    15781 acctggcgcg cgaggtgggc atcgcggaac cggtggtgct gaccggcgcg cagatggacg
    15841 acatgcgcga cgaggcgctg tggcagcggg tggtcgacac cgacctgttc gccgaggtcg
    15901 acccgaacca gaaggagcgc ctgatcctgg ccttgaagcg ctccggccac gtggtggggt
    15961 atctgggcga cggcatcaac gatgcgccgg cgctgcacgc cgccgatgcc ggcatctcgg
    16021 tcgatcaggc ggtggacgtc gccaagcagg cggccgattt cgtgctgctg cagcacgatc
    16081 ttgacgtgct gtgccgcggc atcgagttcg gccgcagcac ctttgccaac acgctcaagt
    16141 acatctacac caccaccagc gccaacttcg gcaacatgat cagcatggcg gcggcgagcc
    16201 tgtttctgcc gtttttgcca ctgctgccga agcagatcct gctgaacaat ttcctctcgg
    16261 acctgccggc gatgggcatc gccaccgacc gggtggacgc ggaggccgtg cagacgccgc
    16321 accgctggaa cgtggccgcc atccgccgct tcatgctcac ctttggcctc atcagctcgc
    16381 tgttcgacgg cctcaccttt gcgctgctgc tgtggctgac cgacggcgca ccggcccact
    16441 ttcgcaccgg ctggttcgtc gagtcgctgc tgaccgagct gtgggtgctg ctgctggtgc
    16501 gcaccgcccg ccccgcctgg cgcagccggc ctggcacgct gctgtggagt ctgacgctgg
    16561 cgctgaccgg cgttgcgatc gcgctgccct acctgccggc gagccacgcg ctgttcgagt
    16621 tcacgccgct gccggcgaac gtgctggcgg cggtgct
//
LOCUS       DAORCQ010000047        16310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2246_length_16310_cov_5.676100, whole genome shotgun sequence.
ACCESSION   DAORCQ010000047 DAORCQ010000000
VERSION     DAORCQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16310
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2246_length_16310_cov_5.676100"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..70)
                     /locus_tag="PKI23_06545"
     CDS             complement(<1..70)
                     /locus_tag="PKI23_06545"
                     /inference="COORDINATES: protein motif:HMM:NF026153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNN36631.1"
                     /translation="MNYCSQCAAPLTRVIPDGDDRLR"
     gene            complement(187..1107)
                     /locus_tag="PKI23_06550"
     CDS             complement(187..1107)
                     /locus_tag="PKI23_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNN36632.1"
                     /translation="MGVLSGKVAVVTGAGNGIGKSMAKYFAKEGAKVVVNDLGGSRDG
                     TGTSRVADQVVDEIKAEGGIAVANYDSVATMQGGLNIFQTAIDNFGQMDILVNNAGIL
                     RDKTIFKMTEEEWDLVIAVHLKGTFTTSQPFARYIRETNRENCRIVNFSSISGLMGNF
                     GQCNYSAAKAGIAGFTRTLAQELAKYKCTVNAFSPVATTRMTADLVAAREGKKEDEIA
                     LDDPLSSGDAIAPIVCWLVSDKGAGITSQVIHGGRGAAGVITQNAMFRLYETDHVWTQ
                     PELDRIFPAIFEEKRAHDAALAAAAQPKKV"
     gene            1320..3374
                     /locus_tag="PKI23_06555"
     CDS             1320..3374
                     /locus_tag="PKI23_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008095586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNN36633.1"
                     /translation="MSSLTTRTLDGLLCCQISATAPQPALLHQLAELIRQAAASPPEA
                     LLLFDQNLLFTLSPELACQPQWLEALDALLAALDQAAMPVIALPQRHLLDVGLELALA
                     CRHRIVTPAARIGMGALQQGLMPGHGGSQRLTRRIGAELALDLLLGGKPLNAEGAKAC
                     GLIDAIDATGGEASAVALVRQLLAAGSGSVAAAQPNPQELATIFERASAQVARSRRGQ
                     FAPQAILQAVRAAVEDSAQGFARERALYLQCRDHPQRAALAHLSRAERFVTHVPDVPA
                     DTSLMPMHRAAVIGSGTMGGGIAMNFANADIPVTLLDVNQEMLDRGLGVIRRNYEIAV
                     QRGSLTQGELERRMGLIGGTTDYGAIAEVDIVIEAVFEDMALKQQVFAQLDQAVKPGA
                     LLATNTSGLDIDQIASATRRPANVIGLHFFSPANVMRLLEIVRGAATSASAIASSMAL
                     AQRMRKVGVLAGNCPGFIGNRMIHGYIFQANQVALEGASYAQVDRVIREFGLPMGPFT
                     MMDLTGLDVGWRARKASGRPTKPTERVHDRLCELERFGQKRSAGFYLYQPGGRVPTYD
                     PLVDQLLADAARECGIARREFSDEEVLDRCIFPLINEGAKILQEGMALRPGDIDVVYV
                     NGYGFPAYRGGPMHHADQLGLDRVYRQICQFEATLGEFWQPAELLRELASAGKGFGSL
                     DP"
     gene            complement(3405..4298)
                     /locus_tag="PKI23_06560"
     CDS             complement(3405..4298)
                     /locus_tag="PKI23_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN36634.1"
                     /translation="MLTLHPDAPDWFRRVLAITPQSRFVEVEGCPIHYLLWGDEGDKP
                     GLLFVHGNGAHAHWWDFIAPFFIDQFRVAAMDLSGMGDSGHRAAYDGDCYASELMSVI
                     DDAGFGDGAIIVGHSMGGFITLRTGALHGDRLTGIVLADAPIRPPDYEWHSSAPIRRR
                     KLYPDFESAVARFRLLPPQPCANDYILDYIARHSLVAVEGGWMWKFDEHFRSPFLYEK
                     RRDDFKNLRCRCGVINADQSSVVTPEVGSYMFRMLDESVPFVTVPETHHHLFLDQPLA
                     FVAALRTMLAEWRHSNPHRGR"
     gene            4447..5757
                     /locus_tag="PKI23_06565"
     CDS             4447..5757
                     /locus_tag="PKI23_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN36635.1"
                     /translation="MKPEPFYGWRLLAALSVIYFLSIGTLYYGLSITLPEFITTFGWS
                     RAEASTGFSITTLSMGLAGPLVAYAMNRFGVRRAMCVGGLVAAVGALNMYFTESRWQY
                     YLVVAPLLGLGIALQSIIPGNYLVSVWFKQRRSLAIGIFMASGGIARAVVVPCFAFVI
                     ETTGNWRLIWLLMALSSLLCAVVSGWLVRERPEDLGQQVDGGVSAEQPSTGRRRLLPT
                     PAAGRVYRTEEEWTLRPALLTTALWMVIFCSAISAAGNSLVNSQGLFHLQDVGIDRIT
                     AASVIGLVGILSTSGRLISGLLGDYIDPRRLLGVGLGLIGLGIVILNLNSSIWGAYLF
                     GCCFGLGNGLALVTTPAILANLFGSRGYASLAATRGVFSTLLSASVPLLAAWSFDLHH
                     NYALAFWSYAAINLLGIGLILAVRPPRLSRQSDQAGGEIEASTS"
     gene            5800..6552
                     /locus_tag="PKI23_06570"
     CDS             5800..6552
                     /locus_tag="PKI23_06570"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNN36636.1"
                     /translation="MGRLSTKVALITGAGAGMGRAHAELMASEGARIIATDIDQAAVE
                     ATCAAIVGRGGEALALQHDVAQESQWTQVVQSGIDRFGKIDILVNNAGVLLSKSLQET
                     TVEEWDRLFSVNVRGVFLGCKSVLPGMQLAGGGSIVNISSIYGLIGAAGSAAYQASKG
                     AVRLMTKAAAVDLRPFNIRVNSVHPGVIRTNMTRAALDNPEATAHISKGILLGRPAEP
                     QEVSAAVLFLASDESSYVHGAELVVDGGYTTV"
     gene            6946..8112
                     /locus_tag="PKI23_06575"
     CDS             6946..8112
                     /locus_tag="PKI23_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36637.1"
                     /translation="MSFTDRSAVVGIGETDYVKGSDRSEIELMLDASRTAIADAGLKP
                     QQIDGIIAPPAYTTVEELAANLGVPDLRYASTINMGGASPNAALGHAAAAIHAGLAKH
                     VLVPLGWNGYTAMRPKPGRPPGRSAEITVLAKTMKDFYLPYGVTAPVQMYAWIATRHK
                     LLHNTPDEATGHIALACRKHAQLNPRAFMREQALSMEQYLSARWISEPFRLFDCCLET
                     DGACAVVVSRADMAKDMRHRPVYIMGAAEGHPYPADDIPSRPDFFRIGLSFAAPKAFE
                     MAGIKPQDVDFLQVYDCFTYVVLLQLEAMGYFAPGGAHDWLKDGQIELGGRYPLNTHG
                     GLLSQAHVWGLNHVVEAVRQLRGDGGAAQVKDAEIGLVTGWGDLGDGSITILRR"
     gene            8120..8548
                     /locus_tag="PKI23_06580"
     CDS             8120..8548
                     /locus_tag="PKI23_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNN36638.1"
                     /translation="MKFDQIPKPVANPTEPLNREFWELCKSGFLHFQRCDDCGRWRHL
                     PRHQCAGCGSPHWHWAVSSGRGKLFSWTISHMVLHPGFAGEAPYAAIVVELEEGVRMV
                     SGLRDLPVDQMRLDMPLEVCFEQVTENFTVPLFRPASAGQ"
     gene            8644..9825
                     /locus_tag="PKI23_06585"
     CDS             8644..9825
                     /locus_tag="PKI23_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36639.1"
                     /translation="MSKGSIRGQVAVVGVGETTYYKRGESPDAEFKLGLQAILKACED
                     AGISPKQIDGFCSFSDDRNDPTRLSAALGIPTMRLSVMQWGGGGGGAAGAISNAAAAI
                     HAGYADVVVAYRALAQGQFGRFGQGSTAAYAQGERAYTAPYGLMSPAQSFAMKVVRFM
                     HDHKIGPNTLRAISMASYHHAQNNPRAIMYGKPLTEEKYDQSRWIVEPYFHLYDCCME
                     NDGAAAIILTSPERARDMKQKPVYLLGAAQGSGHRAGAPVHNAGDYGSSNFTQLTARM
                     YEMAGVTAKDVDVAQIYENFTGGVLMTMIEHNLCTPEEANEFFKLENLVVGGKLPLNT
                     SGGNLAECYMHGLGLQIEAIRQLRGQSTNQVANAKVALAAAGPMVSPVSTCIYGTEEV
                     L"
     gene            9825..10274
                     /locus_tag="PKI23_06590"
     CDS             9825..10274
                     /locus_tag="PKI23_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNN36640.1"
                     /translation="MSANAYYLPVGLPAPAPANDGLDKPYWEGTRAHKLMVQRCNQCK
                     QYQWGPEQICNHCHSFDLGWAEVAGTGRIYSYERIWHPVHPVLKERGPYLVVLIELPQ
                     AGNVRMVGNLLGDGRQEVIIGSAVEAVYEEHNEDPKNPFTLVQWKLV"
     gene            complement(10600..11430)
                     /locus_tag="PKI23_06595"
     CDS             complement(10600..11430)
                     /locus_tag="PKI23_06595"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNN36641.1"
                     /translation="MATYAVGDIQGCYDPLNRLLDACHFDPAADQLWVVGDMVNRGPD
                     SLATLRLLQSLDRCVVAVLGNHDLHLLAVALDNPPQQSNPTLKQLLEAKDRAPLLDWL
                     RQRPFLHEALIETESGPEQMVMTHAGIPPIWSLTEARSHAQEAAELLRGKNHPRYLRG
                     MYGNKPDRWSPQLTGIARFRAITNYFTRMRFCAADGRLELTVKESSAAAPRGFAPWFT
                     LPMRHDEPPYTLLFGHWAALDGHTGRNDRLALDTGCVWGRRMTMLRLEDRHPFTVACR
                     "
     gene            complement(11458..11865)
                     /gene="apaG"
                     /locus_tag="PKI23_06600"
     CDS             complement(11458..11865)
                     /gene="apaG"
                     /locus_tag="PKI23_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNN36642.1"
                     /translation="MRSTDTIPEPIDDRYHIRTAVQTRYLPEQSEPERNRFVFAYTIT
                     LTNEGSIGAQLISRHWIITDGEAQVQEVKGEGVVGEQPHLSPGKSYRYSSGTILGTEV
                     GTMQGSYQMVSDDGTRFDALIAPFTLARPHALH"
     gene            complement(11873..12679)
                     /gene="rsmA"
                     /locus_tag="PKI23_06605"
     CDS             complement(11873..12679)
                     /gene="rsmA"
                     /locus_tag="PKI23_06605"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNN36643.1"
                     /translation="MSNGHRPRKRFGQHFLHDRATLAQIHSSVAPTPADHLVEIGPGT
                     GALTELFAPAARRLDLIEIDRDLVLMLQRKFAALPQLRLHQADALRFDFTRLVIDRPL
                     RIIGNLPYNISTPLLFHLIEQAASIQDMHFLLQKEVVDRLAAAPASADYGRLSVMVQY
                     HCLIEPLFDVPPEAFSPPPRVDSRVVRITPLRQRPVQARDERRLAEVVRVAFTQRRKK
                     IRNALESLIDGNRLQQIGIDPALRPDQLDLLDFVAISDFLTPQDDSARDQ"
     gene            complement(12676..13689)
                     /gene="pdxA"
                     /locus_tag="PKI23_06610"
     CDS             complement(12676..13689)
                     /gene="pdxA"
                     /locus_tag="PKI23_06610"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNN36644.1"
                     /translation="MSNFSSNPQPAAPIALTCGEPAGIGPDLAVMVAQRPTLPPLVAI
                     ADPELLSSRARLLGLPLQLKPWQTGLDAGQHTAGELWVTPLPLKRPARPGQLDPLNSD
                     YVLQSLRDAAEGALSGRFAALVTAPVQKSVINDAGHPFSGHTEFFAELAGVERVVMML
                     ACPQLRVALVTTHLPLCAVPAAITATVLDSTLRIVDAELRRKFGINQPRLLVAGLNPH
                     AGEGGHLGREEIEVITPVLERLRGEGMRLEGPLPADTLFTPPYLARADAVVAMYHDQG
                     LPVLKALGFGEAVNITLGLPFIRTSVDHGTALDLAGTGRARVDSLLAAIDCARQLTGT
                     ARP"
     gene            complement(13670..14911)
                     /locus_tag="PKI23_06615"
     CDS             complement(13670..14911)
                     /locus_tag="PKI23_06615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNN36645.1"
                     /translation="MSPALQAAQQQWLDTTVAIVENDVILASQINRRIDQVAANLRAK
                     GSTPPPREQLYKPVLEQLILESLQLQMADRASIRIDDNSLNQTIDTIAKQNGMDLDTL
                     QKTLEAEGTPFPVFRERIRREMRISRLRQGIIGSGIQISDREVTEFLNSQEGKAQRPT
                     EFHLAHILIALPENALPETVEAARHQADEVAAQLKQGSSFDQLALTQSNASTALEGGD
                     LGWRREAELPSLFSGLVSSMQLGEVRGPIRSPSGFHFIKLLDKRGEPLKQALQLHVRH
                     ILIAPNEIRDPQQSQQLANEIVQRLQRGEAFDELARTYSDDPGSSHAGGDLGWSDPER
                     FAPEFQAAIAELPDGVVSKPFRTRFGWHVARVEGRRSTDISHEYQLNQARELLFRRKF
                     DEELGNWLRKIRDEAYVEFLQ"
     gene            complement(15055..>16310)
                     /locus_tag="PKI23_06620"
     CDS             complement(15055..>16310)
                     /locus_tag="PKI23_06620"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNN36646.1"
                     /translation="LTNLRQAAEAQYRFDDLRLSGLLQSFQSLDAAQISPYRTLPSLT
                     LDYQPLLGQFLLGSLYSNFSYFDRDTGGLTGLDALTGGRLALDLALQRPWRDSFSFVI
                     PSVKLRHRQYQLTDPLANGSDQPELNALSMALDSGLVFERPTQWRGMTATQTLEPRLY
                     LLSTSTSTAQRDFPLFDTSERPLTYTTLFSDNRFVGGDRISDGRQANLAVSTRMLEND
                     GQEAWRLSLGQIFYLKDREVTLQGPPGSSETDGQSAYLGELAVQLNDQSSLLTSLTAD
                     NGLDRIDEALVHLHYAADRQRLGNLGYRLSHDAAGNVIKQSDLSFLYPVGDRVRLLGR
                     WRADIASGSSLEEILGAEYEACCWKLRLVNRRWRREVIALGDTRMESTIELQLILKGL
                     FGLGNRLDELLGSSIQGYPGRDERF"
BASE COUNT         2750 a         5013 c         5537 g         3010 t
ORIGIN      
        1 ggcgcaggcg gtcgtcgccg tcgggaatca ccctggtcag cggggcggcg cactggctgc
       61 agtagttcat gctggggcta tcccgggcga gtgttgccgg gatgctacac gttcggaccg
      121 gatcagttca aaccgcgctg caaatgaatc gggggcgttg ccgcccccga tgggtgtccg
      181 atccgcttac accttcttcg gttgggcggc ggcggccagt gcggcgtcgt gtgcccgctt
      241 ctcctcgaag atcgccggga agatgcgatc gagctccggc tgcgtccaga cgtggtcggt
      301 ctcgtagagg cggaacatcg cattctgggt gatcacgccc gcagcgccgc gtccaccatg
      361 gatcacctgg gaggtgatgc cggccccctt gtcggagacc agccagcaga cgatcggcgc
      421 aatggcgtcg ccgctgctca gtggatcgtc gagggcgatc tcatcttctt tcttgccctc
      481 gcgcgcggcg accaggtcag cggtcatgcg ggtggtggcc accggcgaga aggcgttgac
      541 ggtgcacttg tatttggcca gctcctgcgc cagcgtgcgg gtgaagccgg cgatgccggc
      601 cttggcggcc gagtagttgc actggccgaa gttgcccatc agcccggaaa tcgagctgaa
      661 gttgacgatg cggcagtttt cgcggttggt ttcgcgaatg tagcgggcaa acggctgcga
      721 ggtggtgaag gtgcctttca gatgcacggc gatcaccaga tcccactcct cttcggtcat
      781 cttgaagatg gtcttgtcgc gcagaatgcc ggcgttgttg accagaatgt ccatctggcc
      841 gaagttgtcg atggcggtct ggaagatgtt gaggccgccc tgcatggtgg ccaccgaatc
      901 gtagttggcg acggcaatgc cgccttcggc cttgatctcg tcgaccacct ggtcggccac
      961 ccggctggtg ccggtgccgt cacggctgcc gcccagatcg ttgaccacca ccttggcccc
     1021 ctctttggcg aaatatttcg ccatcgattt gccaatgccg ttgccggcgc cggttacgac
     1081 cgccactttt ccactgagaa cacccatggt ctgactcctg ctggttttat cgttaaaggc
     1141 tctgtcacaa gaacgcccaa gtgtcgttga cgaattcgac tggattcgca cagcatcttt
     1201 cgggtggccc attatagcga agcgcggcag gcggatgtcc gatttcgtgc tggacccccc
     1261 ggcaaggccg ggtatcatcg ctgcggttgt cacgccaatt acaaaataag gactcctcca
     1321 tgtcatcgct caccacccgg accctcgacg gtctgctctg ttgtcagatt tctgccactg
     1381 cccctcaacc ggcgctgctg catcaactgg ccgaattgat acggcaggca gcagccagcc
     1441 ctcccgaagc gctgctgctg ttcgaccaga acctcctctt caccctcagc ccggagctgg
     1501 cctgccagcc gcaatggctc gaggcgctgg atgcactgct ggcggccctg gatcaggcgg
     1561 cgatgccggt catcgcactg ccgcagcgcc atctgctcga tgtcgggctg gagctggcgc
     1621 tggcctgtcg ccatcggatc gtcaccccgg ccgcccgcat cggcatgggc gcactgcaac
     1681 aggggttgat gccgggccat ggcggcagcc agcggctgac gcggcgcatc ggtgccgaac
     1741 tggcgctcga tctgctgctg ggcggcaagc cgctcaatgc cgagggggcc aaggcgtgtg
     1801 gtctcatcga tgccatcgat gcgaccgggg gcgaggcgag cgcagtggcg ctggtgcggc
     1861 aactgctggc ggcagggtcg ggcagcgtgg cggccgcgca gccgaatcca caggagctgg
     1921 ccacgatttt cgagcgggcc agcgcgcagg tggcgcgttc gcggcgtggc cagtttgccc
     1981 cgcaggcgat cctccaggcg gtcagggcag cggtcgagga ttcggcgcag ggatttgcgc
     2041 gtgagcgggc gctctatctg caatgccgcg accatccaca gcgcgcggca ctggcgcacc
     2101 tgtcgcgtgc cgagcgcttc gtcacccatg tgcccgatgt gccggccgac accagcctga
     2161 tgccgatgca cagggcagcg gtgatcggct ccggcaccat gggcggcggc atcgcgatga
     2221 acttcgccaa tgccgacatt ccggtgacgc tgctcgacgt caaccaggag atgctcgacc
     2281 gtggtctggg cgtgatccgc cgcaactacg agattgccgt gcagcgcggc agcctgacgc
     2341 agggtgaact ggagcggcgc atggggctga tcggcggcac caccgactat ggtgcgattg
     2401 ccgaggtcga catcgtcatc gaggcggtct ttgaggacat ggcactcaag cagcaggtct
     2461 tcgctcagct cgatcaggca gtcaagccgg gcgcgctgct ggccaccaac acatccgggc
     2521 tcgacatcga ccagatcgcc agcgccaccc gacggccggc gaatgtgatc ggactgcact
     2581 ttttcagccc ggccaacgtg atgcggctgc tggagatcgt gcgcggtgcc gccacctcgg
     2641 ccagtgccat cgccagttcg atggcactgg cgcagcggat gcgcaaggtg ggggtattgg
     2701 ccggcaattg ccccggcttc atcggcaacc gcatgattca tggctacatc tttcaggcca
     2761 accaggtggc gctcgaaggg gccagctacg cgcaggtcga ccgggtgatt cgcgagttcg
     2821 gcctgccgat ggggccgttc acgatgatgg acctgaccgg cctcgatgtc ggctggcgtg
     2881 cccgcaaggc gagtggccgg ccgaccaagc cgaccgagcg ggtgcatgac cggctttgcg
     2941 aactggagcg tttcggtcag aagcgcagtg cgggcttcta tctctaccag cccggcggcc
     3001 gggtgccgac ctacgatccg ctggtcgatc aactgctggc cgatgccgcc cgcgagtgcg
     3061 gcatcgcgcg acgcgagttc agcgatgaag aggtgctcga tcgctgcatc ttcccgctga
     3121 tcaacgaagg agcgaagatt ctgcaggagg ggatggcgct gcggcccggc gacatcgacg
     3181 tggtctatgt caacggttat ggctttccgg cctaccgggg cgggccgatg caccatgccg
     3241 accagttggg gctcgaccgc gtctaccggc agatctgcca gttcgaggcg acgctgggcg
     3301 aattctggca gccggctgaa ctgctgcgtg agctggccag tgccggcaag ggtttcggca
     3361 gcctcgaccc ctgattcaga cccgcttcgc gacaggtcac cccactaccg tccgcggtgc
     3421 gggttggagt ggcgccactc ggccagcatc gtgcgcagcg cggcgacgaa ggcgagcggc
     3481 tgatccagaa acaggtggtg atgggtctcg ggaacggtga cgaagggcac gctctcgtcg
     3541 agcatccgga acatgtagga gcccacctcg ggggtcacca cgctgctctg gtcggcattg
     3601 atgacgccgc agcggcagcg cagattcttg aagtcatcgc gccgcttttc gtacaggaaa
     3661 ggcgaacgga agtgctcgtc gaacttccac atccagcccc cctccaccgc caccagcgaa
     3721 tggcgggcga tgtagtcgag aatgtaatca ttggcgcagg gttggggtgg cagcagccgg
     3781 aagcgcgcca ctgcgctctc gaagtccgga tagagcttgc ggcgccggat cggcgcgctg
     3841 ctgtgccact cgtagtcggg cgggcgaatc ggcgcatcgg ccagcacgat gccggtgagc
     3901 cggtcgccat ggagcgcgcc ggtacgcagt gtgatgaagc cgcccatgct gtgaccgacg
     3961 atgatggcac catcgccgaa gccggcatcg tcgatcacgc tcatcagctc gctggcgtag
     4021 cagtcgccgt cgtaggccgc ccggtggccg ctgtcgccca tgccgctcag atccatcgcc
     4081 gcgacccgga actggtcgat gaagaaaggg gcaatgaaat cccaccagtg ggcgtgcgcg
     4141 ccattgccat gcacgaacag cagaccgggc ttgtcgccct catcgcccca cagcaggtag
     4201 tgaatcggac aaccctcgac ttcgacgaag cggctctgtg gtgtgattgc cagcacgcgg
     4261 cggaaccagt cgggggcatc gggatgaagc gtcagcatgg cgccggtgtc gtcctgtggg
     4321 ggtaaaattc aaagccggct aggctagcga ctttgtccgg tcaaatccag ccgtggggag
     4381 ggcagctctg tcgccgcggg tcaattttgt taagatcgaa aaaaacaata atcaggaagc
     4441 tgtcgaatga agcctgaacc cttctatgga tggagactgc tcgccgcgct cagcgtcatc
     4501 tactttctgt cgatcggcac cctctactac gggctgtcga tcactctgcc ggagttcatc
     4561 accaccttcg gctggagccg ggccgaggcc agcaccggtt tctccatcac caccctgtcg
     4621 atggggttgg caggtccgct ggtggcctat gcgatgaacc gcttcggcgt gcgccgggcg
     4681 atgtgcgtcg gcgggctggt ggcggcggtc ggcgcgctca acatgtactt caccgaatcg
     4741 cgctggcagt actacctggt ggttgcgccg ctgctcgggc tcggcatcgc gttgcagtcg
     4801 atcattcctg gcaactacct ggtgtcggtc tggttcaaac agcgccgctc gctggcaatc
     4861 ggcatcttca tggcctcggg tggcatcgcc cgggcagtgg tggtgccctg cttcgctttc
     4921 gtgatcgaga ccaccggcaa ctggcggctg atctggctgc tgatggcact gtcgagtctg
     4981 ctctgcgccg tggtcagtgg ctggttggtg cgggagcggc ccgaggatct ggggcagcag
     5041 gtcgatggcg gtgtttcggc cgagcagcca tcgactggca ggaggcggct gctgccgacg
     5101 ccggccgcgg gacgggtcta tcgcactgaa gaggagtgga cgctgcgccc ggcgctgctg
     5161 accacggcgc tgtggatggt gatcttctgt tcggcgatct cggcggccgg taactccctg
     5221 gtgaacagtc aggggctgtt tcaccttcag gatgtcggca tcgaccgcat caccgccgcc
     5281 tcggtgatcg gtctggtggg cattctcagt accagcggcc gactgatctc cggcttgctg
     5341 ggtgactaca tcgatccacg ccgcctgctc ggtgtcggtc tggggctgat cggtcttggc
     5401 atcgtcatcc tcaacctcaa cagcagcatc tggggcgcct acctgttcgg ctgctgcttt
     5461 ggcctgggca acggtctggc gctggtgacc acgccggcga ttctggccaa cctgttcggc
     5521 agtcggggtt atgcctcgct ggccgccacc cgcggtgtct tctcgaccct gctctccgcc
     5581 tcggtgccgc tgctggctgc ctggtcgttt gacctgcatc acaactatgc gctggccttc
     5641 tggagctatg ccgcgatcaa tctgctgggg atcggcttga tcctggcagt gcgaccgccg
     5701 cgactttcac ggcagagcga ccaggctggt ggcgagatcg aagcctccac ctcctgatcg
     5761 aatcgacgga aaaaaccaca acacgaaagg agcctgaaaa tgggacgatt gagcacgaaa
     5821 gtggccctga tcaccggggc cggcgccggc atggggcgcg cccatgccga gctgatggcc
     5881 agtgaagggg cccgaatcat cgccaccgac atcgatcagg cggcggtcga ggcgacctgc
     5941 gccgccatcg tcggccgtgg cggcgaggcg ctggcgctgc agcacgatgt ggcgcaggag
     6001 tcgcagtgga cgcaggtggt gcagagtggc atcgaccgct tcggcaagat cgacatcctg
     6061 gtcaacaacg ctggcgtgct gctgtcgaag agcctgcagg agaccacggt cgaagagtgg
     6121 gaccggctgt tttcggtcaa cgtgcgcggg gtctttctcg gctgcaagag cgtattgccg
     6181 gggatgcagc tcgctggagg tggttcgatc gtcaacatct cctcgatcta tggcctgatc
     6241 ggtgcagcgg gttcagcggc ctatcaggcc agcaagggcg cggtgcggtt gatgaccaag
     6301 gcggcggcgg tcgatctgcg tcccttcaac atccgggtca actcggtcca tcccggcgtc
     6361 atccgcacca acatgaccag ggcggcactg gacaaccccg aggcgacggc ccacatctcc
     6421 aagggcattc tgctgggtcg accggcggag ccgcaggagg tgtcagcggc ggtgctgttc
     6481 ctcgccagcg atgaatcttc ttacgtgcat ggcgccgagc tggtggtcga tggcggttac
     6541 accacggtct gagcggaggg gtgagacata aaaaagggcc atggcgcaat ggccatggcc
     6601 ctgtgacgac accgacgatc atgagacaga gcgattcgag ccaagttcga tccgcgaatc
     6661 tggatgggaa cggatcgcgc tcatgctctg tagtcaagcc tagacggttt cacgcaagcc
     6721 gccagtcgaa ctgccgacaa gcgtcccgcg tgccgtgacc catgccgcga aagtgaccgt
     6781 ttccgcccgg cttgccgcgc atcggccggc tgcgggggga gggaggccgt attgacttgc
     6841 tttccccttt gcgtgtttac ttcggatgga tttgcgttgc tctggcaggc ttgcgggcca
     6901 ccgcgcacag ggcagcggac acaataacaa cgacagggag ttcgcatgag ctttaccgac
     6961 cgctctgccg tcgtcggcat cggtgagacc gattacgtca agggttccga ccgcagcgaa
     7021 atcgagctga tgctcgatgc atcgcgcacc gcgatcgccg atgccggcct caagccgcag
     7081 cagatcgacg gcatcatcgc gccgccggcc tacaccaccg tcgaagagct ggccgccaac
     7141 ctcggcgtgc ccgacctgcg ctatgcctcg acgatcaaca tgggcggtgc cagccccaac
     7201 gcggcgctgg gccatgccgc cgctgcgatt catgccgggc tggccaagca tgtgctggtg
     7261 ccgctgggct ggaatggtta caccgcgatg cggccgaagc cggggcgccc gccggggcgc
     7321 tcggccgaga tcaccgtgct ggcgaagacg atgaaggatt tctatctgcc ttacggcgtc
     7381 accgcaccgg tgcagatgta cgcctggatc gccacccgcc acaagctgct ccacaacaca
     7441 ccggacgagg cgactggcca catcgcgctg gcctgtcgca agcatgccca gctcaacccg
     7501 cgtgcgttca tgcgcgaaca ggcgctgtcg atggagcagt atctctctgc ccgctggatc
     7561 tcggagccct tcaggctgtt cgactgctgt ctcgagaccg atggcgcctg tgccgtggtg
     7621 gtgagccgtg ccgacatggc caaagacatg cgccatcggc cggtctacat catgggtgcg
     7681 gccgagggtc acccctatcc ggccgatgac atcccgtcgc ggcccgattt cttccgcatc
     7741 ggcctcagct tcgctgcgcc caaggccttc gagatggccg gcatcaagcc gcaggatgtc
     7801 gattttctgc aggtgtatga ctgcttcacc tatgtcgtgt tgctgcaact cgaggccatg
     7861 ggttacttcg cgccgggtgg tgcccatgac tggctcaagg atggtcagat cgagctgggt
     7921 ggacgctacc cactcaacac ccatggtggc ctgctgagcc aggcccatgt ctgggggctc
     7981 aaccatgtcg tcgaggcggt gcgccagttg cgtggcgatg gcggtgccgc ccaggtcaag
     8041 gatgccgaaa tcggtctggt gaccggctgg ggcgatctgg gtgacggcag catcacgatt
     8101 ctgcgcaggt gaaacagcca tgaaattcga tcagattccc aagccggtgg ccaaccccac
     8161 cgaaccgctc aaccgtgaat tctgggagct gtgcaaaagc ggcttcctgc acttccagcg
     8221 ctgcgacgac tgcggccgct ggcgccatct gccccgccac caatgcgccg gctgcggctc
     8281 gccccactgg cattgggcgg tgtcgagcgg ccgcggcaag ctcttctcct ggaccatcag
     8341 ccacatggtg ctgcatcccg gctttgccgg cgaggcgccc tacgccgcca tcgtggtcga
     8401 actggaagag ggggtgcgca tggtcagcgg cctgcgtgat ctaccggttg atcagatgcg
     8461 gctcgacatg ccgctcgagg tctgcttcga gcaggtgacc gagaatttca ctgtaccgct
     8521 ctttcggccg gccagcgccg gccagtagaa cggctgcgtg aatcgagtgt ccgggcatgg
     8581 gccgtcgaca ggatggtcca tgcccgggct gccttcacac catgacatga ggatcgatcg
     8641 accatgagca aaggctccat tcgtggccag gtcgccgtgg tgggggtggg tgaaaccacc
     8701 tactacaagc gcggtgaatc ccctgatgcc gaattcaaac tgggtttgca ggccattctc
     8761 aaggcctgtg aggatgcggg aatctccccg aagcagatcg atggcttctg ctccttcagt
     8821 gacgaccgca atgacccgac ccggctgtcg gcggcgctcg gcattccgac gatgcgactg
     8881 tcggtgatgc agtggggtgg cggtggcggc ggcgcggccg gtgcgatctc caacgcggcg
     8941 gcggccatcc atgccggcta tgccgatgtg gtggtggcct accgtgcact ggcccagggc
     9001 cagttcggcc gctttggcca gggctcgacg gcagcctacg cccagggtga gcgcgcctac
     9061 accgcgccct atggtctgat gtcgccggcc cagtcgttcg cgatgaaggt cgtgcgcttc
     9121 atgcatgacc acaagatcgg acccaacaca ttgcgtgcca tctcgatggc ctcctaccac
     9181 catgcgcaga acaacccgcg cgccatcatg tatggcaagc cgctgaccga agagaagtat
     9241 gaccagtcgc gctggatcgt cgagccctac ttccacctct atgactgctg catggagaac
     9301 gatggcgccg cggcgatcat cctcacctcg cccgagcggg cccgcgacat gaagcagaag
     9361 ccggtctatc tgctgggcgc ggcccagggt tccggccacc gtgccggcgc cccggtccac
     9421 aacgccggtg attacggttc gtcgaacttc acccagttga cggcccgcat gtacgagatg
     9481 gccggcgtca ctgccaagga tgtcgacgtc gcgcagatct acgagaactt caccggcggc
     9541 gtgctgatga cgatgatcga gcacaacctc tgcacccccg aagaggccaa tgagttcttc
     9601 aagctggaga acctggtggt cggtggcaag ctgccgctca acaccagcgg cggcaacctg
     9661 gccgagtgct acatgcatgg gttgggtctg cagatcgagg cgatccgcca gttgcgcggt
     9721 caatccacca accaggtggc caacgccaag gtagcactgg cggccgccgg tccgatggtt
     9781 tcaccggtca gcacctgtat ctacggcacc gaggaggttc tctgatgagc gcgaacgcct
     9841 attatctgcc tgttggtctt ccggctcccg cgcccgccaa cgatggcctc gacaagccct
     9901 attgggaagg cacccgtgcc cacaagctga tggtgcagcg ctgcaaccag tgcaagcagt
     9961 accagtgggg accggagcag atctgcaacc actgccactc cttcgacctc ggctgggccg
    10021 aggtggccgg cacgggccgc atctacagct acgagcgcat ctggcacccg gtgcacccgg
    10081 tgctgaagga gcgtggcccc tatctggtgg tgctgatcga actgccgcag gccggcaatg
    10141 tccgcatggt cggcaacctg ctgggcgatg gacggcagga ggtgatcatc ggcagtgccg
    10201 tcgaagcagt ctatgaagag cacaacgaag accccaaaaa tcccttcaca ctggtgcagt
    10261 ggaagttggt gtaatccccg ccttgggctg acgggtcgct ggcccgtcag cctgcttcgg
    10321 ccgatcaggc cgctcctgtt cgcagacaca cgacacgctt tgcccggccg cggtcatcct
    10381 gaacattcgc ggccggcgcg agacataccc cgccgacttt cgtcaatcca ggcctttcag
    10441 ggcgcccatg ctctggaatc ttttttcagt gtcacggctg ttgcgaaatc gctcgacagg
    10501 gatcgatctt ttgggccacc aggaccaatg gaatcacgcg ccgccgtgtg atggcgccgg
    10561 gacgcggtca cgcccgcgtg tggcaagcga cgctggccgt cagcggcagg cgaccgtgaa
    10621 cgggtggcgg tcttcgagcc gcagcatcgt catgcgccgg ccccagacgc agccggtgtc
    10681 gagcgccagc cggtcgttgc ggccggtgtg gccgtcgagg gcggcccagt ggccgaacag
    10741 cagtgtgtag ggcggctcat catgtcgcat cggcagcgtg aaccaggggg cgaaaccgcg
    10801 cggggcggcg gcgctgctct ccttgacggt cagttcgagg cggccatcgg cggcgcagaa
    10861 gcgcatgcgg gtgaagtagt tggtgatggc gcgaaagcgg gcgatgccgg tcagttgcgg
    10921 gctccagcgg tcgggtttgt tgccatacat gccgcgcaga tagcgcggat ggtttttgcc
    10981 gcgcagcagt tctgctgcct cctgggcatg tgatctggct tcggtgaggc tccagatcgg
    11041 cgggatgccg gcatgggtca tcaccatttg ctcaggaccg gattcggtct cgatcagcgc
    11101 ctcgtgcagg aaaggacgct ggcgcagcca gtcgagcagg ggcgcacggt ccttggcttc
    11161 gagcagttgc ttcagggtcg ggttgctctg ctggggcggg ttatcgagcg ccaccgcgag
    11221 caggtgcagg tcatggttgc ccagcacggc caccacgcag cggtcgagcg actggagcag
    11281 gcgcagggtg gccagcgagt cgggaccgcg gttgaccatg tcgcccacca cccagagttg
    11341 atcggccgcc gggtcgaagt ggcaggcgtc gagcaggcgg ttgagtgggt cgtagcaacc
    11401 ctggatgtcg ccgacggcgt aggtggccac tgttcagttc accgtcgagg cgctggatca
    11461 gtgcagcgcg tgtggccgcg ccagcgtgaa cggggcgatg agggcatcga accgggtacc
    11521 gtcgtcgctc accatctggt agctgccctg catggtgccg acttcggtgc cgagaatggt
    11581 gccactgctg tagcggtagc tcttgcccgg tgacagatgg ggctgttcac cgaccacccc
    11641 ttcgcctttc acctcctgca cttgggcttc gccgtcggtg atgatccagt ggcggctgat
    11701 cagttgtgcg ccgatgctgc cctcattggt gagggtgatg gtgtaggcga agacgaagcg
    11761 gttgcgctcg ggttcggact gctcgggcag ataacgggtc tgcactgcgg tgcggatgtg
    11821 gtagcggtca tcgatcggct cggggatggt atcggtcgat ctcatttggg catcactggt
    11881 cacgcgcgct gtcgtcctga ggggtcagaa agtcactgat ggccacgaag tcgagcagat
    11941 cgagctgatc gggtcgcagc gcggggtcga tgccgatctg ttgcagacgg ttgccatcga
    12001 tcagcgattc tagcgcattc ctgatcttct tgcggcgctg ggtgaaggcc acgcgcacca
    12061 cctcggcgag tcgtcgctca tcgcgggcct gtaccggccg ctgccgcagc ggagtgatgc
    12121 gcaccacgcg tgaatcgacc cgcgggggtg gcgaaaaggc ttcgggcggc acgtcgaaca
    12181 gtggttcgat caggcagtgg tactgcacca tcacgctcaa gcggccatag tcggcactgg
    12241 ctggcgcggc ggcgagccgg tcgaccacct ctttctgcag cagaaagtgc atgtcctgaa
    12301 tcgatgcggc ctgttcgatc aggtgaaaga gcagcggcgt ggagatgttg tagggcaggt
    12361 tgccgatgat gcgcagtggt cgatcgatga ccagtctggt gaagtcgaac cgcagggcat
    12421 cggcctgatg gagccgcagc tgaggcaggg cggcgaactt ccgctgcaac atcagcacca
    12481 ggtcgcggtc gatctcgatc agatcaagcc ggcgggcggc gggggcgaac agctcggtga
    12541 gggcgccggt gccgggcccg atctccacca ggtggtcggc cggggtgggc gcaacgctgc
    12601 tgtggatttg cgccagcgtc gcgcggtcat gcagaaaatg ctgaccaaag cgcttgcggg
    12661 gccggtgtcc gttgctcacg gtcgtgcggt gccggtcagt tgtctggcac agtcgatggc
    12721 ggccagcagg ctgtcgaccc gggcccggcc ggtgccggcc aagtcgagtg ccgtgccatg
    12781 gtcgaccgag gtgcggatga agggcaggcc caaggtgatg ttgaccgctt cgccaaaccc
    12841 cagcgccttc agcaccggca atccctggtc gtggtacatc gccaccaccg cgtcggcacg
    12901 ggcgaggtag ggcggagtga agagggtgtc ggccggcagt gggccttcga gacgcattcc
    12961 ctcgccacgc agtcgttcga gcaccggggt gatcacctcg atctcctcgc ggcccaggtg
    13021 acccccttcg ccggcatggg ggttgaggcc ggcgaccagc agtcggggct ggttgatgcc
    13081 gaacttgcgg cgcagctcgg catcaacgat ccgcagcgtg ctgtcgagca cggtcgcggt
    13141 gatcgcagcc ggcaccgcgc acagcggcag gtgggtggtg accagcgcca cccgcagctg
    13201 tgggcaggcg agcatcatca ccacccgctc gacgccggcc agttcggcaa agaattcggt
    13261 gtggccactg aaggggtgtc cagcgtcatt gatcacgctc ttctgcactg gtgcggtcac
    13321 cagtgcggca aagcgaccgc tcagtgcccc ctcggcggcg tcgcgcaggc tctggagcac
    13381 atagtcgctg ttgagcgggt cgagctgacc gggacgcgcc gggcgcttca gtggcagcgg
    13441 tgtcacccac agctctcccg ccgtgtgctg gccggcatcg aggcctgtct gccacggctt
    13501 gagctgcagc ggcagcccca gcagccgggc gcggcttgac agcagttccg ggtcggcaat
    13561 cgccaccagc ggcggcagtg tcgggcgctg cgccaccatg acggcgaggt cggggccgat
    13621 gccggccggc tcgccgcagg tcagcgcaat cggcgctgcg ggctggggat tactggagaa
    13681 attcgacata ggcttcatca cgaatcttgc gcagccagtt gcccagctct tcgtcgaatt
    13741 tgcgtctgaa cagcagttcg cgcgcctggt tgagctgata ttcatgcgag atgtcggtcg
    13801 agcgacgtcc ctccacgcgg gcgacatgcc agccgaagcg ggtgcggaag ggtttgctga
    13861 ccacgccatc gggcagttcg gcgatggcgg cctggaattc gggtgcgaac cgttcggggt
    13921 cgctccagcc gaggtcgccg ccggcgtggc tcgagccggg gtcatcggag taggtgcgtg
    13981 ccagttcatc aaaggcctcg ccccgttgca gccgctgtac gatctcgttg gcgagctgct
    14041 ggctctgctg cgggtcgcga atctcgttgg gggcgatcag aatgtgacgg acatgcagct
    14101 gcaacgcctg cttcagcggt tccccgcgct tgtcgagcag cttgatgaag tgaaaaccgc
    14161 tggggcttcg gatcgggccc cggacctcgc ccaactgcat gctggagacc aggccgctga
    14221 acagcgaggg cagttcggct tcgcgcctcc agcccagatc gccgccttcg agcgcggtcg
    14281 aggcgttcga ctgggtcagc gccaactggt cgaaactgct gccctgcttg agctgtgcgg
    14341 cgacctcgtc agcttgatgc ctggctgctt cgacggtttc gggcagggcg ttttcgggca
    14401 gcgcaatcag aatgtgcgcc agatggaact cggtcgggcg ctgtgccttg ccctcttgcg
    14461 agttgaggaa ctccgtcacc tcgcggtcgc tgatctgaat gccgctgccg atgatgccct
    14521 gccgcagccg cgagatgcgc atctcgcggc gaatgcgttc tcgaaatacc ggaaaagggg
    14581 tgccctcggc ctccagggtc ttctgcaggg tgtcgaggtc catgccattc tgtttggcga
    14641 tggtgtcgat ggtctgattc aggctgttgt catcgatccg gatcgaggcg cggtcggcca
    14701 tctgcagttg caggctttcg agaatcaact gttcgagcac cggcttgtag agctgttcgc
    14761 ggggcggtgg cgtactgccc ttggcgcgca gattggccgc cacctgatcg atccggcggt
    14821 tgatctggct ggccagaatg acatcgttct cgacgatggc gacggtggta tcgagccact
    14881 gctgctgtgc tgcctgcagc gcaggcgaca ccagggaggc cagcgacagg gtcaggggga
    14941 ggagccatcg gccggtcgcg ctggccagac ggagggggga gagaaggagg cgggtaaaat
    15001 ggtggtcaag catggatcga tggtgtccgc gacagaaagg tgaaagtcag aggttcagaa
    15061 tcgctcatca cggccggggt agccctggat gctgctgccg agcagttcgt cgaggcggtt
    15121 gccgagaccg aacagaccct tgaggatcag ttgcagttcg atggtcgact ccatgcgggt
    15181 gtcgccgagg gcaatcacct cgcggcgcca gcgacgattg accagacgca atttccagca
    15241 gcaggcctca tattcggcgc cgaggatctc ctcgagcgaa ctgcctgagg caatgtcggc
    15301 gcgccagcgg ccaagcaggc gaacccggtc gccgaccggg tagaggaagg agaggtcact
    15361 ctgcttgatc acattgcccg cggcgtcatg gctcaatcgg tagccgaggt tgcccaggcg
    15421 ctgccggtcg gcggcatagt gcaggtgaac cagcgcctcg tcgatccggt cgaggccatt
    15481 gtcggcggtg aggctggtga gcagactgga ttggtcgttc agctgcactg ccagttcgcc
    15541 gaggtaggcc gactgaccat cggtctcact gctgccgggt ggaccttgca gggtcacttc
    15601 gcggtccttc aggtagaaga tctgtcccag actgagccgc catgcctcct ggccgtcgtt
    15661 ttcgagcatg cgagtgctga cggccaggtt ggcctggcgc ccatcgctga tgcggtcgcc
    15721 gccgacgaag cggttgtcgc tgaacagggt ggtataggtg agcggccgtt cgctggtgtc
    15781 gaacagcgga aagtcgcgct gcgcagtgct ggtgctggtc gagagcagat agaggcgtgg
    15841 ctccagcgtc tgggtggctg tcatgccacg ccactgggtg ggacgctcaa acaccaggcc
    15901 gctgtcgagt gccatgctca gcgcattcag ttcgggctgg tcgctgccat tggccagcgg
    15961 gtcggtcagt tgatactggc gatggcgcag cttcacgctt gggatcacaa aagagaagct
    16021 gtcgcgccaa ggacgttgca gtgccagatc gagcgcgagt cgcccaccgg tcagggcatc
    16081 cagaccggtg agtccaccgg tgtcgcggtc gaagtaagag aagttgctgt agagcgagcc
    16141 gagcagaaac tgcccgagca acggctgata gtcgagcgtc agactcggca gggtgcgata
    16201 gggagagatc tgtgccgcat ccagactctg aaagctctgc agcagcccgc tcagacgcag
    16261 gtcatcgaag cggtactgcg cttcggcagc ctgccgcagg ttggtcagcg
//
LOCUS       DAORCQ010000048        16157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2275_length_16157_cov_6.603279, whole genome shotgun sequence.
ACCESSION   DAORCQ010000048 DAORCQ010000000
VERSION     DAORCQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_16157_cov_6.603279"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /gene="glmS"
                     /locus_tag="PKI23_06625"
     CDS             complement(<1..1783)
                     /gene="glmS"
                     /locus_tag="PKI23_06625"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNN36647.1"
                     /translation="MCGIVGAVAERPVSEILMEGLRRLEYRGYDSAGIAVIDPACEAV
                     GRHRRQGKVQNLAEALAAMPLAGVVGIAHTRWATHGQPNESNAHPHMSGERLALVHNG
                     IIENHQTLRRELIAEGYRFDSETDTEVVVHLIHQAYQQQPDLQQAVRSAVARLEGAFA
                     LGVIHCDQPHLLVGARKGSPLVVGVGIGENFIASDPLALRQVTDRFIYLEEGDLVTIG
                     AHHLTILDEAGTPVERPVVQLEQPSAAAEKGHFRHFMLKEIYEQPAVIKATFDGRITE
                     EQVLTQLFGVMAEQLFPQVRAVQIVACGTSFHAGLVAKYWIEALAGLPCQVEVASEYR
                     YRKQAVRPGALLVAISQSGETADTLAALRMARQLDYLATLAICNVPASSLVREADLVF
                     MTQAGPEIGVASTKAFTTQLVGLMLLTLVLAKHNGLERAQEREVVQELWRLPMLVEAA
                     LNLDDEIKATAEHFADKRHALFLGRGHQYPVAMEGALKLKEISYIHAEAYPAGELKHG
                     PLALVDSEMPVITVAPNNELLEKLKSNLQEVKARGGELYVFADRAARAFDEENCHVVA
                     VPEVSDLLAPIIYTIPLQLLAYHVAVLK"
     gene            complement(1817..3172)
                     /gene="glmU"
                     /locus_tag="PKI23_06630"
     CDS             complement(1817..3172)
                     /gene="glmU"
                     /locus_tag="PKI23_06630"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNN36648.1"
                     /translation="MTLNVVILAAGQGTRMRSAKPKVLHPLAGRPLLAHVLAAAEALP
                     QPKIHLVVGHGADQVRASLANARVDWVEQAQQLGTGHAVMQALPLLDPDAQTLILYGD
                     VPLITPATLTALLQQLTADAVALLTVRLPNPTGYGRILRNPAGEVVAIVEEKDATPAQ
                     RAIDEVNTGIMALPTALLARWLPQLSTGNAQGEYYLTDLVAMAVAEGKPIATLHPASF
                     DEVTGVNTRQQLAELERSYQRQQANALMAAGVSIIDPARFDLRGRCQAGEDVTLDVNV
                     VLEGDVTLGDGCTIGPNCLIRNSVLAAGVVVEANSLIDGAVIGPECTIGPFARIRPGT
                     RLAAKVRVGNFVETKNAVVEQGSKMNHLSYIGDATIGAGVNIGAGTITCNYDGKSKHR
                     TTIESGVFVGSNTALVAPVTIGRDATVAAGSTITKEVPAEALGIARGQQRNVDGWKSR
                     R"
     gene            complement(3262..3681)
                     /locus_tag="PKI23_06635"
     CDS             complement(3262..3681)
                     /locus_tag="PKI23_06635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNN36649.1"
                     /translation="MAMTIQCEVVSAEERIFSGLVESVVATAALGELGVNYGHAPLLS
                     TLKPGPVRLLTPAGEEQFIYVSGGFIEVQPGMVTILADSAVRAADLDEAAAEEARRQA
                     EEALGGRHVEFDYALAELTQAVAQLRTLRRMKKTPGG"
     gene            complement(3730..5106)
                     /gene="atpD"
                     /locus_tag="PKI23_06640"
     CDS             complement(3730..5106)
                     /gene="atpD"
                     /locus_tag="PKI23_06640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNN36650.1"
                     /translation="MSSGKIIQIIGAVIDVEFPRDQIPKVYDALTIDGTDTTLEVQQQ
                     LGDGVVRTIAMGSTEGLRRGLDVTDTGQPVLVPVGKETLGRIMDVLGRPIDEAGEIGE
                     KARMPIHRTAPGFADQAATNELLETGIKVIDLICPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFTGVGERTREGNDFYHEMKESNVLDKVALVYGQMNEPPGNRLRV
                     AFTGLTMAESFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGV
                     LQERITSTKVGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRKIAELGIYPAID
                     PLDSTSRQLDPLVVGEEHYEVARGVQNTLQRYKELKDIIAILGMDELSETDKLTVSRS
                     RKIERFLSQPFFVAEVFTGSPGKYVPLKETIRGFKGILSGEYDHLPEQAFYMVGSIEE
                     AIEKAKKL"
     gene            complement(5147..6007)
                     /gene="atpG"
                     /locus_tag="PKI23_06645"
     CDS             complement(5147..6007)
                     /gene="atpG"
                     /locus_tag="PKI23_06645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNN36651.1"
                     /translation="MASGKEIRKQISSVKSTQKITNAQQMVAVSKMRKATDRMAAGKP
                     YATHIRAVIGHLANSSAEYRHTYLEEREVKRVGYIVVTSDRGLCGGLNINLFKALTQE
                     MAQWSRQGVGIDLCVIGNKGQAYFNSYGGNLLASVSRLGDTPVIADLVGPIRVMLDAY
                     TAGKVDRVVLAGNTFINTMTQKPTLNQLLPLQAEDAQQYKHRWDYIYEPDAKELLDGL
                     LTRFIESQVYQAVVENFACEQAARMVAMKSASENAAELINELQLAYNKVRQAGITQEL
                     AEIVGGAAAV"
     gene            complement(6044..7588)
                     /gene="atpA"
                     /locus_tag="PKI23_06650"
     CDS             complement(6044..7588)
                     /gene="atpA"
                     /locus_tag="PKI23_06650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNN36652.1"
                     /translation="MQQLNPAEISEIIRQRIAKFDIGAEARNEGTIVSVSDGVVRIHG
                     LADVMYGEMIEFPGGLYGMALNLERDSVGAVVLGEYKGLTEGQTARCTGRILEVPVGP
                     ELIGRVVDPLGNPIDGKGPLNHKMTDAIEKVAPGVISRKSVDQPVQTGLKVIDAMVPI
                     GRGQRELIIGDRQVGKTAIALDAIINQKGTGVICIYVAIGQKQSTIANAVRKLEEHGA
                     MDHTIIVAAGAADPASTQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNAEYVEQFTQGEIKGQTGSLTALPIIETQ
                     GGDVSAFVPTNVISITDGQIYLESDLFNAGIRPAMNAGLSVSRVGSAAQTKIIKKLGG
                     NIRLSLAQYRELAAFSQFASDLDDATREQLEHGKRVTELMKQKQYSPMSVAEMGIVLY
                     AVNEGFLKDIPVDQVVNFEAALLSYLRAEQSALLSQINEKGDYNDQIAASIRSAIETF
                     KKTQSW"
     gene            complement(7604..8146)
                     /locus_tag="PKI23_06655"
     CDS             complement(7604..8146)
                     /locus_tag="PKI23_06655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014861150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNN36653.1"
                     /translation="MAELTTLARPYARAAFESALAETSLADWSQVLAFLAVASSDPKV
                     GQLLGSPRLTAGNQVAMLVDLASEQVTISQSVKNFLGLLAEQKRLPLLPQIAVLFEIL
                     RAEQEKSVDVTVTSAHPLSQAQLQKITEALYRRFNKSIHIQAHLDSALIGGVTIRAGD
                     LVIDGSVKGKLSRLAEAMNT"
     gene            complement(8160..8630)
                     /locus_tag="PKI23_06660"
     CDS             complement(8160..8630)
                     /locus_tag="PKI23_06660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNN36654.1"
                     /translation="MNINATLLGQMIAFALFTWFCMKYVWPPMMAALTERQKKIAEGL
                     DAASRAEHDLELAKQRVVEQLREGKDQAAAIIEQANRRGISIIEEAKEQARREVDRIK
                     EGARAEIEQELMQAKEALRTQVAKLAVAGAERILQKAVNDEVDAELIDRLAGEL"
     gene            complement(8674..8934)
                     /gene="atpE"
                     /locus_tag="PKI23_06665"
     CDS             complement(8674..8934)
                     /gene="atpE"
                     /locus_tag="PKI23_06665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNN36655.1"
                     /translation="METVIGFTAIAVALLIGMGALGTAIGFGILGGKFLEGAARQPEM
                     APMLQVKMFIVAGLLDAVTMIGVGIALFFTFANPFVGQLAAG"
     gene            complement(8965..9786)
                     /gene="atpB"
                     /locus_tag="PKI23_06670"
     CDS             complement(8965..9786)
                     /gene="atpB"
                     /locus_tag="PKI23_06670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNN36656.1"
                     /translation="MAEGALTSSEYIKHHLQNLAFGRFPDGHWGLAHSAQEAADFGFW
                     AIHVDTMGWSIGLGLLFILFFRSVAKKASSGVPGMAQNIVEMAVEFVDTSVKESFHGQ
                     SKLIAPLALTIFCWILMMNCMDLLPVDLLPRIAGLLGVHYFKVVPTTDLNATLGMSIS
                     VFLLIVFYSIKIKGLGGFVGELTLQPFGKWMMPFNLLLEGVGLLAKPVSLGLRLFGNL
                     YAGELIFILIALLPLFAQWPLSVPWAIFHILIVVLQAFIFMMLTIVYLSMAHEHH"
     gene            complement(9795..10229)
                     /locus_tag="PKI23_06675"
     CDS             complement(9795..10229)
                     /locus_tag="PKI23_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HNN36657.1"
                     /translation="MIHGQLRGRQRRSHGTSTIGKPAFQRVVLAQSVATLVAGLIGAG
                     VDWIWAYSALLGGFTAVIPNGYFAFRVFAHQGAQSAARIVRAFYWGEAAKLGLTAAMF
                     CLVFVLVRPLSVAALFGAFVVVQAVGWAASARLAAVAPKRDV"
     gene            complement(10370..11248)
                     /locus_tag="PKI23_06680"
     CDS             complement(10370..11248)
                     /locus_tag="PKI23_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNN36658.1"
                     /translation="MTSKKRQGLGRGLDALLGKSASIASEATGQQGELRELPIDQLQR
                     GRYQPRREMDPEALEELAQSIRSQGIMQPIIVRPLTHDQHEIIAGERRWRAARLAGLD
                     TVPVLVREIPDEAAVAMALIENIQREDLNPLEEAQALHRLSNDFQLTHQELAALVGKS
                     RATVTNLLRLLALADEVKRLLENGDLEMGHARALLSLDAARQREAARLIVAKGLSVRQ
                     TEDLVRSLTDNAGNHRGKKLPAAQVQPDIQRLEENLSERLGAAVSIQHRQSGKGSLVI
                     RYNTLDELDGILAHLQ"
     gene            complement(11255..12031)
                     /locus_tag="PKI23_06685"
     CDS             complement(11255..12031)
                     /locus_tag="PKI23_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNN36659.1"
                     /translation="MTRIIAITNQKGGVGKTTTSVNLAASLAATRKRVLLVDLDPQGN
                     STMGSGVEKNQLASSIYDLLMREQPFDRVVLGNLQVGYDLIPSNEDVTAAEVELIQQP
                     DREQRLRESLTAVRDRYDFILIDCPPSLSMLTVNALAVADGVIIPMQCEYYALEGLSS
                     LLNTLDRIRQKLNPPLQIDGLLRTMYDPRNSLTQEVSRQLHEHFGDRLLRTVIPRNVR
                     LAEAPSHGLPVLLYDKNSKGATAYLALAGELLRRLDDTGD"
     gene            complement(12053..12694)
                     /gene="rsmG"
                     /locus_tag="PKI23_06690"
     CDS             complement(12053..12694)
                     /gene="rsmG"
                     /locus_tag="PKI23_06690"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNN36660.1"
                     /translation="MTEAQNERQLLSEGLTRIQGEPPLPAVVDLLLQYAQRLRLWNRR
                     FNLISRGDEEKLVSRHLLDSLILLPFIDRQPIIDLGSGAGLPGIPLAICRPELSITLL
                     DSNGKKTRFLTQVKTELQLEQLQVVHARMEQHRPPVAFNLLLSRAVSDVATLIEQSSQ
                     LAAPAARYLLLKGTYPQHELENIPPTHQVVAVEPLKVPGLSATRHLVIVQKRE"
     gene            complement(12702..14594)
                     /gene="mnmG"
                     /locus_tag="PKI23_06695"
     CDS             complement(12702..14594)
                     /gene="mnmG"
                     /locus_tag="PKI23_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNN36661.1"
                     /translation="MQHPSRFDVIVVGGGHAGTEAALAAARMGCRTLLLTQNIETLGQ
                     MSCNPAIGGIGKSHLVKEIDALGGAMAQATDRAGIQFRVLNSRKGAAVRATRAQADRV
                     LYRAAIRQTIENQPNLLLFQQAVDDLILSGDRVSGVITQMGLRFSASTVVLTAGTFLG
                     GVIHIGLQHHQGGRAGDPASTTLAARLREYPLPVGRLKTGTPPRIDGRTVDFSVMQPQ
                     PGDQPTPVMSYLGRAAQHPAQVCCHITHTNARTHDIIRGGLDRSPMYTGVIEGVGPRY
                     CPSIEDKITRFADKSSHQIFVEPEGLNTHELYPNGISTSLPFDVQLELVHSIKGFENA
                     HISRPGYAIEYDYFDPRGLHQSLETRTIEGLFFAGQINGTTGYEEAAAQGLLAGINAA
                     LKVREQPAWTPRRDQAYLGVLVDDLITRGTIEPYRMFTSRAEYRLLLREDNADMRLTA
                     IGRQLGLVDEERWRHFNEKCEAIECERSRLTTTWIRPNTAEASAIEALIGEPLLREYS
                     LATLLKRPELGYQALLTVLGETPQWDAASEQVEIEIKYEGYIKRQQQEIERLQRQESM
                     VIPDDLDFNQITGLSNEVKQKLTQARPDSLSRASRIPGVTPAAISLLLIHLKKQALRQ
                     SAAGSR"
     gene            14948..16012
                     /locus_tag="PKI23_06700"
     CDS             14948..16012
                     /locus_tag="PKI23_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNN36662.1"
                     /translation="MRNSDQADANIKNKSFLLVLLLVTLAFTVMLLPFYGALFWAAVL
                     AIIFQPIQRMLIRRHPRHQNLTALLTLLICLFLAIIPVVLISISLVNEGQLLYQKVQS
                     GNVNVGAYLDKVRGLIPTGLRPLIERYGLENNLDLKSKITEALMQSSQFIANQALSIG
                     QNAFKFILSIGLMLYLLFFFIRDGVTILERIRQALPLEETKKSRLFGMFATVVRATIK
                     GNLIVAATQGLLGGIIFSILGIEAAVLWGVLMAFLSLVPAVGSGLIWLPTSLYLLLSG
                     EIWRGIVLILFGLFVIGLVDNLLRPILVGKDIKLPDYLVLLSTIGGIVLVGMNGFVIG
                     PLIAALFVSCWSLLQDDALG"
BASE COUNT         2915 a         5099 c         4948 g         3195 t
ORIGIN      
        1 ccttgagcac ggccacatgg taggccagca gttgcagcgg gatggtgtag atgatcggcg
       61 ccagcaggtc gctcacctcc ggcacggcga ccacgtggca gttctcttcg tcgaaggcgc
      121 gcgccgcgcg gtcggcaaag acgtagagtt cgcctccgcg cgccttcacc tcctgcaggt
      181 tggacttcag cttctccagc agctcgttgt tgggcgccac ggtgatgacc ggcatctcgc
      241 tgtcgaccag cgccagcggg ccatgcttga gttcgccggc cggataggcc tcggcgtgga
      301 tgtaggagat ctccttgagc ttgagcgccc cctccatcgc caccggatat tgatggccac
      361 ggccaaggaa gagggcatgg cgcttgtcgg cgaagtgctc ggcggtcgcc ttgatctcgt
      421 catccaggtt gagcgcggcc tcgaccagca tcggcagtcg ccacagctcc tgcaccacct
      481 cgcgctcctg cgcccgctcc aggccgttgt gtttggccag caccagggtc agcagcatca
      541 gcccgaccag ctgagtggtg aaggccttgg tcgaggcgac gccgatctcg ggtccggcct
      601 gggtcatgaa caccaggtcg gcctcgcgga ccagtgaact ggccggcacg ttgcagatcg
      661 ccagcgtggc caggtagtcg agctgccgcg ccatgcgcag ggccgccaga gtgtcggcgg
      721 tctcgccgga ctgggagatg gccaccagca gggcaccagg acgcaccgcc tgcttgcggt
      781 agcggtactc gctggccacc tcgacctgac aggggaggcc ggccaatgcc tcgatccagt
      841 acttggccac cagtccggca tggaaactgg tgccgcaggc gacgatctgc accgcccgca
      901 cctggggaaa gagctgctcg gccatgacgc cgaacagctg ggtcagcacc tgctcttcgg
      961 tgatcctgcc gtcgaaggtg gccttgatca ccgccggctg ttcgtagatc tccttcagca
     1021 tgaagtggcg gaagtggccc ttctcggccg ccgcgctcgg ctgttccagt tgcacgaccg
     1081 ggcgttcgac cggggtgccg gcctcatcga gaatggtcag gtggtgggcg ccgatggtca
     1141 ccagatcgcc ctcttcgagg tagatgaagc ggtcggtcac ctggcgcagc gccagcggat
     1201 cggaggcgat gaagttttca ccgatgccga caccgaccac cagcggactg cccttgcgcg
     1261 cccccaccag cagatggggc tggtcgcagt ggatcacacc gagtgcaaag gctccctcca
     1321 gccgcgccac cgcgctgcgc accgcctgtt gcagatcggg ctgctgctgg taggcctgat
     1381 ggatcaggtg caccaccact tcggtgtcgg tttcggaatc gaagcggtaa ccctcggcga
     1441 tcagttcgcg gcgcagcgtc tggtggttct cgatgatgcc gttgtgcacc agcgccagcc
     1501 gctcgcccga catgtgcgga tgggcattcg actcattggg ctggccatgg gtcgcccagc
     1561 gggtgtgggc gatgccgacc acgccggcca gcggcatcgc cgccagcgcc tcggccaggt
     1621 tctgcacctt gccctgacgc cgatggcggc caaccgcttc gcaggctgga tcgatcaccg
     1681 cgatgccggc ggagtcatag ccgcggtatt cgaggcgacg cagcccctcc atcaggatct
     1741 ccgacaccgg ccgttcggcc acggctccaa cgattccaca catggttctg ttccactaag
     1801 gggttgagtg actgcttcag cgacgcgatt tccagccgtc gacgttgcgc tgctgaccac
     1861 gggcgatgcc gagcgcctcg gcaggcacct ccttggtgat ggtcgatccc gccgccaccg
     1921 tggcatcgcg gccgatcgtc accggcgcca ccagcgcagt gttggagcca acgaagacgc
     1981 ccgactcgat ggtggttcga tgcttcgatt tgccatcata gttgcaggtg atggtgccgg
     2041 caccgatgtt gacgccggca ccgatggtgg catcgccgat gtagctcaga tggttcatct
     2101 tgctgccctg ttcgaccacg gcattcttgg tctctacgaa gttgccgacc cgcaccttcg
     2161 ccgccagccg ggtgccgggg cgaatgcggg caaaggggcc aatggtgcat tcagggccga
     2221 tcacggcacc atcgatcagg ctgttggcct cgaccaccac gcctgccgcc agcacgctgt
     2281 tgcgaatcag gcagttgggg ccgatggtgc aaccgtcgcc gagggtcacg tctccttcga
     2341 gcaccacatt gacatcgagg gtgacatcct ctcccgcctg gcagcgacca cgcaggtcga
     2401 aacgggccgg atcgatgatc gacaccccgg cagccatcaa ggcattggcc tgctggcgct
     2461 ggtagctgcg ctccagttca gccagctgct gccgggtgtt gacgccggtc acctcatcga
     2521 aactggccgg atgcagtgtg gcaatcggct tgccctcggc caccgccatc gccaccagat
     2581 cggtcaggta gtactcgccc tgggcattgc cggtactcag ttgcggcagc cagcgggcga
     2641 gcagggcagt gggcagggcc atgatgccgg tgttcacctc gtcgatggcg cgctgggccg
     2701 gtgtggcgtc cttctcttcg acgatggcga ccacctcacc cgccggattg cgcaggatgc
     2761 gcccatagcc ggtcggattc ggcagcctga ccgtcagcag cgccactgcg tcagcggtga
     2821 gctgttggag cagggcggtc aaggtggccg gcgtgatcag cggcacatca ccatagagaa
     2881 tcagcgtctg tgcgtcggga tcgagcagcg gcagcgcctg catcaccgca tggcccgtgc
     2941 ccagttgctg cgcctgctcg acccagtcga cacgcgcatt ggccagactc gcccgcacct
     3001 ggtcagcgcc atggccgacc accagatgaa ttttaggttg tggcaacgcc tcggcagcgg
     3061 ccagtacatg ggccagcagc gggcgacccg ccagtggatg cagcaccttg ggcttggccg
     3121 agcgcatccg tgttccctgg cccgcagcca ggatgacgac attcaatgtc atgcaggagc
     3181 ctctgaaacg aaaaagggtg gccgtggcca ccctttcaag tttacgagat aaatcgaatc
     3241 ttgctgaggc ttcagccaga ttcaaccacc cggtgttttc ttcatccgcc gcaaggtgcg
     3301 cagctgcgcc accgcctggg tcagctcagc cagggcgtag tcgaactcga cgtgacgtcc
     3361 acccagcgcc tcttcggcct gtctgcgcgc ctcttcggcc gcagcttcat ccagatcggc
     3421 cgcgcgcacg gcgctgtcgg ccagaatggt caccatgcca ggctgcactt cgatgaaacc
     3481 acccgagaca tagatgaact gctcttcgcc ggcgggtgtc agcagtcgca ccggacccgg
     3541 cttgagcgtc gacagcagcg gggcatggcc atagttgaca cccagctcgc ccagcgcggc
     3601 ggttgccacc accgactcca ccagtccgga aaagatccgc tcttcagcgc tgacgacttc
     3661 gcactggatt gtcattgcca tggcttgttt ctctcgcgca aaaggttatc cgcggtcatc
     3721 cgatgcagct cagagcttct tcgccttctc gatcgcctcc tcgatgctgc caaccatgta
     3781 gaaggcctgc tcgggaaggt ggtcatattc gccggaaaga atgcccttga agccacgaat
     3841 ggtctccttc agcgggacat acttgccggg tgaaccggtg aagacctcgg cgacgaagaa
     3901 gggttgcgac aggaaacgct cgatctttct cgaccgggag acggtcagct tgtcggtctc
     3961 ggagagctca tccatcccca gaatggcgat gatgtccttc aactccttgt agcgttgcag
     4021 ggtgttttgc acgccacgcg ccacttcgta gtgctcttcg ccgaccacca gcggatcgag
     4081 ctgacggctg gtggagtcga gcgggtcgat cgccgggtag atgccgagct cggcgatctt
     4141 ccgcgacagc accacggtcg catcgaggtg ggcaaaggtg gtggcaggcg acgggtcggt
     4201 caggtcatcc gctggcacat agaccgcctg gatcgaggtg atcgagccga ccttggtcga
     4261 agtgatgcgc tcctgcagca cgcccatctc ttcggccagc gtcggctggt agcccaccgc
     4321 cgagggcatc cgccccagca gcgcggagac ctcggtgccg gccagcgtgt agcggtagat
     4381 gttgtcgacg aacagcagca cgtcgcgccc ctcgtcacgg aaactttcgg ccatcgtcag
     4441 accggtgaaa gccacgcgca gacggttgcc gggcggctcg ttcatctggc catagaccag
     4501 cgccaccttg tcgagtacgt tcgactcctt catctcgtgg tagaagtcgt tgccctcgcg
     4561 ggtgcgctcg ccgacaccgg tgaagaccga gtagccgctg tgttcgatgg cgatgttgcg
     4621 gatcagctcc atcatgttga cggtcttgcc gacgccggcg ccgccgaaca gaccgacctt
     4681 gccgcccttg gcgaatgggc agatcaggtc gatcaccttg atgccggttt cgagcagctc
     4741 gttggtcgcc gcctgatcgg caaaaccggg agcggtacgg tgaatcggca tccgcgcctt
     4801 ttcgccgatt tcgccggcct cgtcgatcgg ccgacccagc acatccatga tgcgccccag
     4861 ggtctccttg ccgaccggca ccagcaccgg ctggccggtg tcggtcacat ccagtccgcg
     4921 ccgcagccct tcggtgctgc ccatggcgat ggtgcgcacg acgccatcac ccagctgctg
     4981 ctgcacttcg agcgtggtgt cggtgccatc gatggtgagt gcatcgtaga ccttggggat
     5041 ctggtcacgg ggaaactcga cgtcgatcac cgcgccaatg atctggatga tttttccgct
     5101 gctcatgtgc ttgtttcctc aatcaaatct gaataattca tccgcctcat acggcggctg
     5161 cgccaccgac gatttcggcc agctcctggg tgatgccggc ctgacgcacc ttgttgtagg
     5221 ccagctgcag ctcgttgatg agctcggcgg cattctcgct ggcgctcttc attgccacca
     5281 tgcgcgcggc ctgctcgcag gcgaaattct cgaccaccgc ctgatagacc tgcgactcga
     5341 tgaaccgggt cagcagccca tcgagcagct ccttggcatc gggttcatag atgtagtccc
     5401 agcggtgctt gtactgctgc gcatcctcgg cctgcagcgg caggagctgg ttcagcgtcg
     5461 gcttctgggt catggtgttg atgaaggtgt tgccggccag cacgacccgg tcgaccttcc
     5521 ccgcggtgta ggcatcgagc atcacccgga tcggacccac cagatcagcg atcaccgggg
     5581 tgtcgccaag ccggctgaca ctggccagca ggttgccgcc gtagctgttg aagtaggcct
     5641 gacccttgtt gccgatgacg caaaggtcga tgcccacccc ttgacggctc cactgcgcca
     5701 tctcctgggt cagtgccttg aacaggttga tgttgagccc accgcacaga ccacggtcgc
     5761 tggtgacgac gatgtagccg acccgcttca cctcccgctc ttcgaggtag gtgtgacggt
     5821 attcggccga cgaattggcc agatggccaa tcaccgcacg aatgtgggtc gcatagggct
     5881 tgccggccgc cattcgatcg gtggccttgc gcatcttgct caccgccacc atctgctgcg
     5941 cattggtgat cttctgcgtg ctcttgacgc tggaaatctg cttccgaatc tctttgccac
     6001 tggccatcgc tgagctgtct ctggagggtg acgccctggc tggttaccag gattgggtct
     6061 tcttgaaggt ctcgatcgcg ctgcgaatgc tggcggcgat ctggtcgttg tagtcaccct
     6121 tttcgttgat ctggctcagc agcgcactct gctcggcacg caggtaggag agcagggcgg
     6181 cctcgaagtt caccacctga tcgaccggaa tgtccttcag gaacccttcg ttgaccgcat
     6241 agagcacgat ccccatctcg gccacgctca tcggcgagta ctgcttctgc ttcatcagct
     6301 cggtgacgcg cttgccatgc tccagctgtt cacgggtggc atcgtcgaga tccgacgcaa
     6361 actgcgagaa ggccgccagt tcgcgatact gcgccagaga cagacgaatg ttgccgccca
     6421 gcttcttgat gatcttggtc tgggccgcgc tgcccacccg cgacaccgac aggccggcat
     6481 tcatcgccgg acggatgccg gcgttgaaga ggtcggactc gagatagatc tgaccatcgg
     6541 tgatcgagat cacgttggtc ggaacgaacg ccgagacgtc accgccctgg gtttcgatga
     6601 tcggcagtgc ggtcagtgag ccggtctggc ctttgatctc gccctgggtg aactgctcga
     6661 catattcggc attgacccgt gccgcccgct cgagcaggcg cgagtgcaga tagaagacgt
     6721 cacccggata ggcttcacgg cccggcggac ggcgcagcag cagggagatc tgccgatagg
     6781 cccacgcctg cttggtcagg tcgtcataga tgatcagggc atcctggccg cggtcgcgga
     6841 agtattcgcc catggtgcaa ccagagtaag gggcgatgta ctgggtcgag gccgggtcgg
     6901 ccgcaccggc ggccacgatg atggtgtgat ccatcgcgcc atgctcttcg agtttgcgca
     6961 cggcattggc gatggtcgac tgcttctggc cgatcgctac gtagatgcag atgacgccgg
     7021 tacccttctg gttgatgatg gcatcgagcg caatcgccgt cttgccgacc tggcggtcgc
     7081 cgatgatcag ttcgcgctgg ccacgtccga tcggcaccat ggcatcgatc accttgagac
     7141 cagtctgcac cggttggtcg accgatttgc ggctgatcac cccgggcgcg accttttcga
     7201 tcgcatcggt catcttgtgg ttgagtggac ccttgccgtc gatcgggttg ccgagcggat
     7261 cgacgacgcg gccgatcagc tccggaccca ccggcacttc gagaatgcgg ccggtgcagc
     7321 gggcggtctg accttcggtc agccccttgt actcacccag caccacggca cccaccgaat
     7381 cgcgctccag gttgagggcc atgccataga gccccccggg gaattcgatc atctccccgt
     7441 acatcacgtc agccagacca tggatgcgca cgaccccatc ggagaccgac acgatggtgc
     7501 cttcgttgcg ggcctcggcc ccaatgtcga acttggcgat ccgctgcctg ataatctcgc
     7561 tgatttcggc tgggttcagc tgctgcatgc tcttttcctc aaatcaggtg ttcatcgctt
     7621 cggcaagtcg actgagcttg ccttttaccg agccatcaat gaccagatca ccagcgcgta
     7681 tcgtcactcc gccgatcagc gccgaatcca gatgggcttg aatatgaatg gatttgttga
     7741 agcgccggta cagcgcctcg gtgattttct gaagctgcgc ctggctcagc ggatgggcgc
     7801 tggtgacggt gacatcgacc gacttctctt gctctgcccg cagaatctcg aacagcacag
     7861 cgatctgcgg cagcaatggc aggcgtttct gctcagccag cagacccaga aagttcttga
     7921 ccgactgact gatggtgacc tgctcgctgg ccagatcgac cagcatggcc acctggttgc
     7981 cagcggtgag ccgtggcgac ccaagaagct gcccgacctt cggatcgctg ctggccaccg
     8041 ccagaaaggc gagcacctgc gaccagtcgg ccagacttgt ttcagccagc gcactctcga
     8101 aagcggctct ggcgtaggga cgggcaaggg tggtgagctc agccattggg tgactccggt
     8161 tacagctcgc cagccagacg gtcgatcaac tctgcatcga cttcgtcgtt gaccgccttt
     8221 tgcagaatcc gctcggcacc ggccaccgcc agtttcgcca cctgggtgcg caatgcctcc
     8281 ttggcctgca tcagctcctg ctcgatctcg gcacgagctc cctccttgat tcggtcaact
     8341 tcgcggcgtg cctgctcctt ggcctcttcg atgatcgaaa taccccgtcg gttggcctgc
     8401 tcgatgatcg cggctgcctg atccttgcct tcgcgaagct gctcaaccac ccgctgcttg
     8461 gccagctcca ggtcatgctc ggcgcgggaa gcggcatcga gcccctcggc aatcttcttc
     8521 tgccgttccg tcagcgcagc catcatcggc ggccacacgt acttcatgca gaaccaggta
     8581 aagagggcga aggcaatcat ttgcccaagc agggttgcat tgatattcac accgacacct
     8641 cagcaggcag aggggaaaac tgatgacgcg agatcaacca gccgccaatt gaccaacgaa
     8701 aggattggcg aaggtgaaga acagggcgat acccacaccg atcatggtca cggcgtcgag
     8761 cagacccgca acgatgaaca tcttcacctg cagcatcggc gccatctctg gttggcgggc
     8821 cgcgccctca aggaacttgc cacccagaat gccgaacccg atcgcggtgc ccagggcacc
     8881 cataccgatc agcagtgcga cagcgatagc ggtaaagcca attacagtct ccatggtttc
     8941 tcccgtttgt ggtcaaagaa aaggtcagtg gtgctcgtgc gccatgctca gatagacaat
     9001 ggtcaacatc atgaagatga atgcttgcag caccacgatc aggatgtgga agattgccca
     9061 gggtaccgac agcggccact gtgcaaaaag cggcagcagc gcaatcagga tgaagatcaa
     9121 ctcaccggca tagaggttgc cgaagagtcg aagaccaagg ctgaccggct tggccagcag
     9181 acccacccct tccaacagca ggttgaaagg catcatccac ttgccaaagg gctgcaatgt
     9241 cagctcaccg acaaacccac ccagcccttt gatttttatg ctgtaaaaaa caatcagcag
     9301 aaatacggag atcgacatgc ccaaggtggc attgaggtcg gtggtgggca ccaccttgaa
     9361 gtagtgcaca cccagcaggc cggcgatcct tggcagcagg tcgaccggca gcaggtccat
     9421 gcagttcatc atcagaatcc agcagaagat ggtcaacgcc aacggcgcga tcagcttgct
     9481 ctgaccatga aagctctcct tgacgctggt gtcgacaaac tcgaccgcca tttcgacgat
     9541 gttctgcgcc atgcccggca cgcctgatga agcctttttg gccaccgatc gaaagaacag
     9601 gatgaacagc agccccagac cgatcgacca acccatggta tcgacatgga tcgcccagaa
     9661 gccgaaatcg gcggcctcct gcgcggagtg ggccaagccc caatgaccat cggggaagcg
     9721 accgaaggcc aggttctgca ggtgatgctt gatgtattca gaggatgtga gagcgccttc
     9781 tgccatgact taccttatac atctcgttta ggggcaaccg cagccagcct tgccgatgca
     9841 gcccagccca ccgcctgcac aaccacgaat gcgccaaaca gcgcagccac gctcagcggc
     9901 ctgaccagta caaaaaccag acagaacatt gccgcggtca aaccgagctt cgccgcttca
     9961 ccccaataga atgcgcggac gatccttgcc gcagactgcg cgccctgatg cgcgaacacc
    10021 ctgaaggcaa agtagccatt gggaatcacc gcggtgaatc cgccaagcag ggccgaatag
    10081 gcccagatcc aatccacacc ggcgccgatg aggccagcca ccagggtggc aacgctctgg
    10141 gcaagaacca cgcgctggaa tgccggtttg ccgatcgtgg atgttccatg cgagcgcctt
    10201 tgtcggcctc gcaactggcc atgaatcatg atcgaaccac tctgcgcgtc gtgccctgct
    10261 caggcggccg cgattatagg gggattggca gacccattca aggaagaaat tggcgattca
    10321 agccattgca gctgtttctt cgtgctcggc ggcggccaag cagaatgcat cactgcagat
    10381 gcgccaggat gccatccagc tcgtccaggg tgttgtagcg gatgaccaga ctgcctttgc
    10441 cactctgacg gtgctgaatc gacaccgcag caccgaggcg ctcggagagg ttctcttcga
    10501 gccgctggat gtccggctgc acctgggctg ctggcagctt tttgccacgg tggttgccgg
    10561 cattgtcggt gaggctgcgc accagatctt cggtctggcg caccgagagc cccttggcga
    10621 cgatcaggcg cgccgcttcg cgctgtctgg ccgcatcgag cgacagcagt gcccgggcgt
    10681 ggcccatctc caggtcgcca ttttcgagca gtctcttcac ctcatcagcc agcgccagca
    10741 gtcgcagcag attggtgacg gtcgcgcgcg atttgcccac cagcgcggcc agctcctggt
    10801 gggtgagctg aaagtcgttg ctgagccgat gcagggcctg cgcctcttca agcggattga
    10861 gatcttcgcg ctggatgttt tcgatcagcg ccatcgcgac ggcggcctca tcgggaatct
    10921 cccggaccag caccggaaca gtgtcgagac cggccagccg ggccgcgcgc cagcgccgct
    10981 cgccggcgat gatctcatgc tggtcatggg tcagtggacg gacgatgatc ggctgcatga
    11041 tgccctggct gcggatcgac tgcgccagct cttccagtgc ctcagggtcc atctcgcgcc
    11101 ggggctgata gcgtccgcgc tgaagctggt cgatcggcag ttcgcgcagc tcaccctgtt
    11161 ggccggtcgc ctcgctggca atactggcgc tcttgcccag tagcgcatcc agcccacggc
    11221 ccagcccctg cctcttcttg ctcgtcatcg tcgatcaatc cccggtgtcg tccagtcggc
    11281 gcagcagctc tccggccagg gccagatagg cggtcgcacc cttggagttc ttgtcgtaca
    11341 gcaacacagg caggccatgg ctgggcgctt cggccagccg cacattgcgt ggaatcaccg
    11401 ttctcaacag cctgtcgccg aaatgttcat gcaactggcg cgagacctcc tgggtcaggc
    11461 tgttgcgggg gtcatacatc gtgcgcagca gtccatcgat ctgcagtggc ggattgagct
    11521 tctgccgtat ccggtccagc gtgttgagca gcgagctgag cccctccagc gcatagtact
    11581 cgcactgcat cggaatgatg accccatcgg ccactgccag cgcgttcacc gtcagcatgc
    11641 tgagcgaggg ggggcagtcg atcagaatga agtcgtagcg gtccctgacc gcggtcaggc
    11701 tctcgcgcag ccgctgctca cggtcgggct gctggatcag ctcgacctcg gcagcggtga
    11761 catcctcgtt ggagggaatc aggtcatagc ccacctgcag gttgcccagc accacccggt
    11821 cgaacggctg ctcacgcatc agcaggtcgt agatcgagct ggcgagctga tttttctcga
    11881 cgccgctgcc catggtgctg ttgccctgcg gatcgagatc gaccagcagc acccgctttc
    11941 tggtcgcggc aagcgaagcc gccagattga cgctggtggt ggtcttgccc accccgcctt
    12001 tctggttggt gattgcgatg atgcgcgtca tctgctggcc tggttgcctc tgttattctc
    12061 ttttttgcac gatcaccagg tggcgggttg cgctcaatcc tggcactttc aagggttcaa
    12121 ccgcgaccac ctggtgggtt ggcggaatgt tttccagctc atgctgcgga taggttccct
    12181 tcaaaagcag atagcgggcc gccggcgccg ccaattggct gctctgttcg atcagggtcg
    12241 ccacgtcact aacggcacgg ctcaacagca gattgaacgc aaccggcggt cgatgctgct
    12301 ccattctggc atgaaccacc tgcaactgtt ccagctgcag ctcggttttg acctgggtca
    12361 gaaaacgggt tttcttgccg ttgctgtcga gcagcgtgat cgacagctcg ggacggcaga
    12421 tcgccagcgg aatgccgggc agaccggcgc cgctgcccag gtcgatgatc ggctgccgat
    12481 cgatgaaggg caacaggatc agtgaatcga gcagatggcg tgagaccagc ttctcttcat
    12541 caccgcggct gatcaggttg aagcggcggt tccacagccg cagacgctgt gcatactgca
    12601 gcagcaggtc gaccaccgcc ggcaaggggg gctcaccctg aatccgggtc agtccctcgc
    12661 tcagcagttg ccgttcgttc tgcgcctcgg tcatcacacc ctcagcggga acccgcagcg
    12721 ctctggcgca gggcctgctt tttcagatgg atcagcagca gcgagatcgc cgccggcgtg
    12781 acgccgggta tccgtgatgc acgtgacagg ctgtcgggcc gggcctgggt caatttctgc
    12841 ttcacttcat tcgacaatcc ggtgatctgg ttgaagtcga ggtcgtcggg aatcaccatc
    12901 gactcctgcc gctgcaaccg ctcgatctcc tgctgctgcc gcttgatgta gccctcgtac
    12961 ttgatctcga tctcgacctg ctcgctggcc gcatcccact gcggcgtctc gccgagcacg
    13021 gtcagcagag cctgataacc cagttcaggc cgcttgagca gggtcgccag gctgtattct
    13081 cgcaacagcg gctcgccaat cagcgcctcg atggcgctgg cctcggcggt gttcgggcga
    13141 atccaggttg tggtgaggcg gctgcgttca cactcgatcg cctcgcactt ttcgttgaag
    13201 tggcgccagc gctcttcatc gaccaggcca agctggcgcc cgatcgcagt cagacgcatg
    13261 tcggcgttgt cctcacgcag cagcaggcgg tattcggcgc ggctggtgaa catccggtag
    13321 ggctcgatgg tgccgcgggt gatcaggtca tcgaccagca cacccagata ggcctggtca
    13381 cggcgtggtg tccaggccgg ctgctccctg accttgagtg ccgcattgat gccggccagc
    13441 agcccctggg ccgcagcctc ctcgtagccg gtggtgccat tgatctgccc ggcaaagaac
    13501 aacccctcga tcgtgcgggt ctccagtgat tgatgcaggc cgcgcgggtc gaaatagtca
    13561 tactcgatcg cataaccggg ccggctgatg tgggcgttct cgaagccttt gatcgaatgg
    13621 accagctcca gctgcacatc gaacggcagg ctggtggaga tgccgttggg atagagctca
    13681 tgggtattca gcccctccgg ctcgacgaaa atctggtggc tgcttttgtc ggcaaagcgg
    13741 gtgatcttgt cctctatcga cgggcagtag cgtggaccca ccccttcgat cacaccggtg
    13801 tacatcggcg agcggtcgag tccgccgcgg atgatgtcgt gggtacgggc gttggtgtgg
    13861 gtgatgtggc agcagacctg cgctggatgc tgcgccgcac gacccaggta ggacatcacc
    13921 ggtgtcggct ggtcaccggg ctgcggctgc atcaccgaga agtcgactgt gcgcccatcg
    13981 atgcgcggcg gtgtaccggt cttgagccga cccaccggaa gtgggtactc acgcagccgt
    14041 gcagccagcg tggtcgaagc gggatcgccg gcgcgtccac cctggtggtg ctgcaggccg
    14101 atgtgaatga cgccgcccag aaaggtgcca gcggtcagca ccaccgtgct ggcgctgaag
    14161 cgcaagccca tctgggtgat gacgccactc acccggtcac cgctcagaat caggtcatcg
    14221 accgcctgct ggaacagcag caggttgggc tggttctcga tggtctggcg aatggcggca
    14281 cggtagagca cgcggtcggc ctgcgcccgg gtggcccgca ctgcagcacc tttgcggctg
    14341 ttgagcacac gaaactggat gccggcgcgg tcggttgcct gcgccatggc gccccccagc
    14401 gcatcgatct ccttcaccag atggctcttg ccaatgccgc caatggccgg attgcaggac
    14461 atctgcccca gggtttcgat gttctgggtc agcaacaggg tccggcagcc catgcgggcg
    14521 gctgccagcg ccgcctcagt gccggcatga ccgccgccca cgacaatgac atcgaaccgg
    14581 ctggggtgtt gcatggcgcg catcgacctg agaaaagcaa aagggcagcg attatagaac
    14641 tccgccaagc caaagcgaaa ttgccggact gacagatatt attctgtttt gatttaaaag
    14701 atggttgtgg ttgttaggct cgccctggcc tgtgagcaag ttttcaacac catgatttca
    14761 agtaattttt ctctttttgg ctgttgttgg taaagtggcc tttgcagtgg gggcgagttg
    14821 gggataaccg cagcgcatcg ctggcactgc ccgctgtgac cgatcgatgc aacggttgcc
    14881 cacaacgaaa cagccgcgct ttacccagct tgtccacaac cgaatccgaa catcatccga
    14941 gcccatcatg cgcaacagtg accaggccga cgccaacatc aagaacaaga gctttctgct
    15001 ggtgctgttg ctggtcacgc tggccttcac ggtgatgctg ctgcctttct atggagcgct
    15061 gttctgggcc gcagtgctgg cgatcatctt ccagccgatt cagcggatgt tgatcagacg
    15121 ccacccccgg catcagaacc tcactgcgct gctgacgctg ctgatctgcc tgtttctggc
    15181 gatcatcccg gtggtgctga tctcgatctc gctggtcaat gaggggcaac tgctctacca
    15241 gaaggtgcag tcgggcaatg tgaatgtcgg tgcctacctc gacaaggtgc gcggactgat
    15301 tcccacgggg ttgcgaccac tgatcgagcg ctacgggctc gagaacaatc tggacctgaa
    15361 gagcaagatc accgaggcgc tgatgcagag cagccagttc atcgccaacc aggcgctcag
    15421 catcggccag aatgccttca agttcatcct gagcatcggc ctgatgctct acctgctgtt
    15481 tttcttcatc cgtgatggtg tgacgatcct cgaacgcatc cggcaggcac tgccactcga
    15541 agagaccaaa aagagccggc tgttcggcat gtttgccacc gtggtgcgcg ccaccatcaa
    15601 gggcaacctg atcgtggccg ccacccaggg cctgctgggc gggatcatct tctcgatcct
    15661 tggcatcgag gctgcggtgc tctggggtgt gctgatggcg ttcctgtcgc tggtgccggc
    15721 ggttggctcc ggcctgatct ggctgccgac ctcactctat ctgctgctga gcggtgagat
    15781 ttggcgcggc atcgtgctga ttctgtttgg cctgttcgtc atcggtctgg tcgacaacct
    15841 gctgcggccg attctggttg gcaaggacat caaactgccc gactatctgg tgctgctctc
    15901 caccatcggc ggtatcgtgc tggttggcat gaacggtttc gtgatcggcc cgctgatcgc
    15961 cgcgctcttc gtctcctgct ggtcactgct gcaggatgat gcgctgggtt gaggggggtt
    16021 gtgcaggaga gcgcctgttt cgtgtgccgc gcagttccaa cagatcggag cacgaaaaca
    16081 gcgacagacc acggttcgtt caatatcgaa gttttggaaa gcgccttgat caggaacagg
    16141 aaaccgtggt ctgtccc
//
LOCUS       DAORCQ010000049        16031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2297_length_16031_cov_5.734164, whole genome shotgun sequence.
ACCESSION   DAORCQ010000049 DAORCQ010000000
VERSION     DAORCQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2297_length_16031_cov_5.734164"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..548
                     /locus_tag="PKI23_06705"
     CDS             267..548
                     /locus_tag="PKI23_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNN36663.1"
                     /translation="MEIAFDPDKSARNLAKHGVSLALAATLEWEWLLAKSDSRQDYGE
                     ARMIGYAPIGDRIFCVVFVDRDATRRIISLRRANSREVKRYAQRIQDET"
     gene            556..801
                     /locus_tag="PKI23_06710"
     CDS             556..801
                     /locus_tag="PKI23_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008055048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNN36664.1"
                     /translation="MPSSKEDAAINAGIASDEETYELSDDEFAQTRRMGRPVAVRTKE
                     RITIRLSPEVVAHFRATGRGWQTRMDEALKEWLKTHP"
     gene            complement(829..3045)
                     /locus_tag="PKI23_06715"
     CDS             complement(829..3045)
                     /locus_tag="PKI23_06715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNN36665.1"
                     /translation="MSFKDEMRVALDTFIVESRELLQQMEEALLGLEQAEEVAEAIDA
                     IFRTVHTIKGSAGLFGLTHIVEFTHVVESVLDALRDGRIEVSAELIATLLPCHDHIGL
                     LIEAVAAGGDADEGPQLAAGTALLARLQPLVSGVIQAVTPAEQGTTRIDTSGGPLRSG
                     NWHLSLRFAEECLRNGMDPFSFIRYLATLGDIIHLATLTDSLPSATAIDPECCYLGFE
                     IDFHSGASKEEIESVFEFVLDGSTVRILPPDSQVSDYIELIRSLPEEEARLGEILVKS
                     GILTQRELDEGLRLQQQSSAAGTPHQPIGAILVEQQMVQQPVVEAVLERQQQIKEKKA
                     REAQSIRVDAERLDRLIDLVGELVIAGAGANLRASVVADAALNEATAEVMRLVEAVRD
                     SALQLRMVPIGTTFSRFQRVVRDVSSELGKEIELVIRGADTELDRSVVEKIGDPLTHL
                     VRNAMDHGIESTERRLAAGKPARGRLMLNACHDAGSVVIEVGDDGGGLNRDRILAKAI
                     DRGIVAPNANLSDHDIHQLIFAAGFSTADQVSNLSGRGVGMDVVKRNIEALRGTVELE
                     SVPGEGTTVRIRLPLTLAIIDGFLVGVGDAAYVVPLGMVLECVELAEADRQALTHRRY
                     VNLRGEVLPFVRLRDHFEIGGNPGRRENIVVVQYGGQKAGVVVDRLMGEFQTVIKPLG
                     KIFSRIKGISGSTILGSGEVALILDVPALIQQAVAREAGLTGASPDDAPHRVLAAG"
     gene            complement(3057..3356)
                     /locus_tag="PKI23_06720"
     CDS             complement(3057..3356)
                     /locus_tag="PKI23_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNN36666.1"
                     /translation="MQRLAFEGEMTIYHAAEQKARLLAFLDSGDELEIDLAAVDEIDT
                     TGLQLLIMAKQQARRGGKRLGFVLHSPAVIELFELANLVNFFDDPLVLSHRPDRA"
     gene            complement(3383..3751)
                     /locus_tag="PKI23_06725"
     CDS             complement(3383..3751)
                     /locus_tag="PKI23_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN36667.1"
                     /translation="MAKSIMIVDDSASLRQVVGIALKGAGYEVVEACDGKDALTKLDG
                     RKIHLIISDVNMPNMDGITLVKEVKKLPSYRFTPVIMLTTESQEGKKAEGQAAGAKAW
                     VVKPFQPAQMLQAVSKLILP"
     gene            complement(3775..4953)
                     /locus_tag="PKI23_06730"
     CDS             complement(3775..4953)
                     /locus_tag="PKI23_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNN36668.1"
                     /translation="MAENLKSKEQARFLLPCRVTPALLGLAAALTVWAVHPADSLALL
                     SSTLLLLAGCVVGVLLGRQYRKTVAPLQADADAEPTAVSRTEEQQLQFERFCGDALPI
                     WMRQIDMAQSQTEQAITALSSRFANLYDHISGGVVASQENQDSSLNALLQHSSGELQV
                     VMDALHAVLQAQKTTAASIQQLAAFTGELQQMTGEVGKIATQTQLLALNASIEAARAG
                     EAGRGFSVVADEVRKLSLRSGETVERINAKVAAVSGAIHHTLTLSETTSQQHADRVSS
                     AETRIAGVLDQFEQTASTLLDSAQALQAQNRMIGDELSEVLVSLQFQDRVKQILGHVC
                     DDLDALQQWLERDQGSREPLDARRWLEESSRRYTMVDQHQAHAGRAVAAPQSNEITFF
                     "
     gene            5143..5976
                     /locus_tag="PKI23_06735"
     CDS             5143..5976
                     /locus_tag="PKI23_06735"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNN36669.1"
                     /translation="MNDANSLSGLTVALAESRELDLLARMVEERGGRVWRCPLVAIVD
                     APDEAAVQAWIGRMIAREFDLFILFTGEGLRRLLAAAGRSQREAALIAALADTEILCR
                     GPKPVRELRRLGLKPQHQASAPTTQGVIETLEQLDLRGRRITLQLYGSDPNPPLIDHL
                     AARGIVPVTVAPYAYAPKSDEMQVVALIDALAQGGVDALLFTSQPQYRRLVEVAQRHD
                     RGTQLAAGLERIKVGAIGPVMADLLHQQGVRVDFMPEARFFMKPLVSSLVRHTEGGEP
                     A"
     gene            complement(5986..6840)
                     /gene="ttcA"
                     /locus_tag="PKI23_06740"
     CDS             complement(5986..6840)
                     /gene="ttcA"
                     /locus_tag="PKI23_06740"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNN36670.1"
                     /translation="MEIEQLSKRDRFEFNKLQKRLRRNVGRAIEEYNMIEAGDRVMVC
                     LSGGKDSYTLLDILLNLRDHAPLDFELIAVNLDQKQPGFPQEVLPGYLSRLGVPFHIL
                     ERDTYSIVKSIVPEGKTTCGLCSRLRRGNLYSFAQQIGATKIALGHHRDDIIETLFLN
                     LFHGGKLKAMPPKLLSDDGRNVVIRPLAFCTEEDIAAYARLKAFPIIPCNLCGSQQNL
                     QRQKVKEMLQQWERQQPGRLDVIFAAIRNVAPSQLADVRLFDFVNLTATAAPAAAADD
                     DRLQLLQL"
     gene            complement(6919..8088)
                     /locus_tag="PKI23_06745"
     CDS             complement(6919..8088)
                     /locus_tag="PKI23_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNN36671.1"
                     /translation="MNRGPRELLIAALLGLLVGLLLLFNSPDWLRKPEPVPAPAPSAA
                     PPAAPPPVSEPHRETPPAVVASYAEAVKRAAPAVVNVYTRKRSAATSAYLDDPLLARI
                     HGGNDEARRQRMQSSLGSGVIVSHKGDILTNHHVVANAEDILVLLRDGREAAARLVGA
                     DPETDLALLKIELTGLQPIELADGKQIEVGDVVLAIGNPFGVGQSVTFGIIGATGRAG
                     LGLSTFENFIQTDAAIHPGNSGGALVNAEGRLVGINSAVYSQQGGGSLGIGFAIPAAV
                     AYQVMQALIDHGRVVRGWLGIEVERVELPGAATARIEGLRVTAVVSGSPAEQAGIAEN
                     DIITRLSGRESWNGRLAMEQIARARPGEQVRISVLRGSEPMELTATVGERPPAEP"
     gene            complement(8144..8581)
                     /locus_tag="PKI23_06750"
     CDS             complement(8144..8581)
                     /locus_tag="PKI23_06750"
                     /inference="COORDINATES: protein motif:HMM:NF018049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HNN36672.1"
                     /translation="MITLQFWQAISIFVVAVLLGGAMGWLLALRQGGSKAQHDARALE
                     REFDQYRDEVSSHFVQTSELVGRLSESYRDVFDHLASGAQRLCNGEAVERIVIQNRIQ
                     KERLTERLPLSDELPLTPPRDYAPKRHPDEKGLLADDYGLDQP"
     gene            complement(8763..9929)
                     /locus_tag="PKI23_06755"
     CDS             complement(8763..9929)
                     /locus_tag="PKI23_06755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36673.1"
                     /translation="MDFTLSQEHQLIVDSADKIAQKFDRKYWLECAKHKRFPQEMWNE
                     LGSMGYLGITCPEEYGGAGMGMTELALLQERLASHGVALLYFVVNQGIAMPCISKHGT
                     PEQKQRWLPAMASGEKRCCFAITEPNAGTNTFKIQTLGKLDGGDYVLNGSKLFISGIN
                     QADHVLVVARTQSYAEVGQDKKYEGLTLFVVDTKSPGLTFDPMDTMLQNTEGQYFVYF
                     NNVRVPKENVIGQPGKGLAYLFDALNPERIVVAAGAVGGGRWCLKRGVEYANQRVIFD
                     RPIGAYQGLQHPLADAAALLEVAWLMTLKAAWQQDNGENAGAAANIAKYTASEASYRA
                     ADAALQVHGGYGFTESMDMLHSFVGARLGKVAPINREMTLNYIGQHLLGLPKSY"
     gene            10378..11307
                     /locus_tag="PKI23_06760"
     CDS             10378..11307
                     /locus_tag="PKI23_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN36674.1"
                     /translation="MYLTGKSALVTGGGRGIGRAVALRMAAEGAAVVVMDPGVGRDGR
                     GAEGAPASEVAEEIRAAGGQAVPCHEPVDDHAAAERAVHLAVEAFGRLDIVVNCAGVL
                     RERMIWNLTEDDWDTVIRVHLKGHYNINHHASKLMRSQQSGRIINFSSDAWRGSVGQV
                     NYAAAKGGIVSMTRGLARELGRYGVTSNAICPMAATRMTLNEGVIAGMKKRLEAGLIT
                     PERFAAMQAMPGPEYVAPFVAYLASDHAAHINGQVFHVEKGRVSIYSEPVEERALYKQ
                     ENDGHFTLDDLIAQVPQTLLNGYHNPAPRESQP"
     gene            11339..11884
                     /locus_tag="PKI23_06765"
     CDS             11339..11884
                     /locus_tag="PKI23_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNN36675.1"
                     /translation="MAEKQVGTNMPLGTVAEGQAMVGKRSRTRFHTMDVNWPMIRIYA
                     SAMEDGNPCYWDVDYARRRFGDIVSPPGMLMVWGMGLDWQPDAPVVADDPPLMNIPLP
                     GTTIINVVTETEFFRPIRVGERLNAQEELVSVSDEKSTRLGVGCFIRTQTSYRNQAGE
                     LVALHTNDVYRYRPHDKEVTP"
     gene            11881..12342
                     /locus_tag="PKI23_06770"
     CDS             11881..12342
                     /locus_tag="PKI23_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNN36676.1"
                     /translation="MTHSIAGAFGKKYLYRTWGEVEVGQALPEVRMPISYRRVVQMTV
                     ATRDFFPGHHNREYARNQGVDDIYLNTMTYHGLIDRYVLEWAGPESFLRKRSMRMGTS
                     ICAGDEVVMNGRVTAKEREGEHGLVVVEMVVANRAGVPCCPATVRLWMPDQ"
     gene            12362..13072
                     /locus_tag="PKI23_06775"
     CDS             12362..13072
                     /locus_tag="PKI23_06775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNN36677.1"
                     /translation="MTTITTIDLQRLGLKPDDRLLDVGCGLGRHTLAAYRHCSLLAVG
                     VDLSLADLQQAQHRFGDFDQDSPHRSALWAQAHGARLPFADASFDKVICCEVLEHLDR
                     PEAVISELVRVLKPDGLLAVSVPSYWPERLCWLLSRDYPADVGHLRIFCGDELPRAIE
                     ASGLRCIAEHREHGLHSPWWWLQALLWSRREQSWLVRRYKDFLDWALFRSPAWLRRLE
                     LRLNPLLGKSRVFYFTRR"
     gene            complement(13082..14887)
                     /locus_tag="PKI23_06780"
     CDS             complement(13082..14887)
                     /locus_tag="PKI23_06780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNN36678.1"
                     /translation="MRDAATETPFSLTGTLDAIPNRAISRPMEALLERVLSLKKLDRL
                     YSALAPCSDQQLFLQQLFELFNIHYQVADEELARIPQSGPLVVVANHPFGAIEGVIMA
                     ALLRQVRPDVKIMANFVLKRIPELRDLFIAVDPFGGAGAARSNIGPMKEALRWLKQGG
                     LLMVFPAGEVSNLKPFRGIEDSTWSRSIGRLIQLAQAPAQPVYFHGANSLLFQLAGLM
                     HPLLRTVMLPRELLNKTNTTIPIRIGKVQPWSRLKQIEEDQALIDYLRMRTYMLRNSA
                     KPAGGQRERGQTSGCALIEPVECALLRQEVDALPEAQRLVDGGALQVHCATAGQIPHL
                     LREIGRLRELSFRAAGEGTGRALDLDAYDAYYLHLFIWNRDTDEVVGAYRLGESDRIV
                     ASRGVAGLYSHSLFRYSRRLIDQLNPALELGRSFVRQEYQKSFAPLLLLWKGIATFMH
                     RNPRYRRLFGPVSISSDYQPLSQKLLVDFLRIHNSQPTLAKLVKPRSPFRARAGKAVD
                     SARAHLNSVEAVDDLIAELEVDGKGIPVLLRQYLKFGGQILAFNVDDQFNNSLDGLIM
                     VDLLQTEPRTLARYMGKAETAEFHAWHGGESRQAG"
     gene            complement(15208..15546)
                     /locus_tag="PKI23_06785"
     CDS             complement(15208..15546)
                     /locus_tag="PKI23_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNN36679.1"
                     /translation="MSNFPCCPKCNSAYTYEDGGQYVCPECGHEWSTASKEAVEDRRV
                     WKDAFGNELKDGDSVTVIKDLKVKGSSSVVKVGTKVKNIRLVDGDHDIDCKIEGFGSM
                     QLKSQYIKKV"
     gene            complement(15873..>16031)
                     /locus_tag="PKI23_06790"
     CDS             complement(15873..>16031)
                     /locus_tag="PKI23_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36680.1"
                     /translation="ALHLVLAHLLVAAEEGGHFPAQAQLCDKCHFKAKVLLDGCLTCL
                     NCGDSKCG"
BASE COUNT         2877 a         5241 c         4970 g         2943 t
ORIGIN      
        1 tcgtttccca tcgcaacacc ttaacaaggt gttgcacttg attattgagc cggcttcctt
       61 tgggtttttc cgcttcaatc aacagcgatg ctgctgagac atttctgcag ttcagccgcc
      121 tgcgcggtct gacaggcacg ttttgtcgtc gccgctccaa tcgacgacga caattccggg
      181 agcacggccg cattgacggc ccaaccccct gaattccggg tggtcacggc ttgtgagccg
      241 ggtttcttgt acatacaatc aggccaatgg agatcgcgtt cgacccggac aagagtgccc
      301 gcaatctcgc caaacatggc gtttcgctgg ctttggcggc aaccctggaa tgggagtggc
      361 tgctggccaa atccgattcc cgccaggatt acggcgaggc gcgcatgatc ggctacgcac
      421 caatcggcga ccggattttc tgcgtggtgt tcgtcgatcg tgacgcaacg cgccgcatca
      481 tcagcctgcg tcgggccaac agcagagagg taaaacgtta tgcccagcgt atccaggacg
      541 aaacctagat tcatcgtgcc ctcgtccaag gaggatgccg ccatcaacgc cggcattgcc
      601 agcgatgagg agacatacga gctctcggac gacgagttcg cgcaaacccg acgcatgggc
      661 cgccctgtcg cggtccggac caaggagcgc atcaccattc gcctgtcgcc cgaggtggtg
      721 gcacacttcc gcgccaccgg ccgcggctgg cagacgcgca tggacgaggc gctgaaggag
      781 tggctgaaga cgcatccgta gcctgacatc gcctgtcgct ccactgcttc agcctgccgc
      841 cagcacgcgg tggggtgcat cgtcgggact ggccccagtg aggccggctt cgcgcgccac
      901 tgcctgctga atcagcgctg gcacatccag aatcagcgcc acctcgccgc tgccgaggat
      961 ggtggaaccg gagatgccct tgatccgtga gaagatcttg cccagcggct tgatcacggt
     1021 ctggaattcg cccatcagcc gatcgaccac cacgccggcc ttctggccac cgtactgcac
     1081 caccacgatg ttctcgcggc gcccggggtt gccgccgatc tcgaaatgat cgcgcagccg
     1141 cacgaagggc aacacctcgc cacgcaggtt gacataacgg cgatgggtca gcgcctggcg
     1201 gtcggcctcc gccagctcga cgcactccag caccatcccc agcggcacca cgtaggcggc
     1261 atcgccgacg ccgaccagaa agccatcgat gatcgccagc gtcagcggca gacggatgcg
     1321 cacggtggtg ccctcgccgg gcacactctc cagctcgacg gtgccgcgca gcgcctcgat
     1381 gttgcgcttc accacatcca tgccgacacc gcggccggag aggttgctca cctggtcggc
     1441 ggtggagaaa ccggccgcga agatcagctg atggatgtcg tggtcgctga gattcgcgtt
     1501 gggcgcgacg atgccgcggt cgatcgcctt ggccaggatt ctgtcgcggt tcaggccgcc
     1561 accgtcatcg cccacttcga tgacgacgct tccagcgtcg tggcaggcat tgagcatcaa
     1621 tcggcccctg gccggtttgc cggccgccag tcgccgctcg gtcgattcga tgccatggtc
     1681 catggcgttg cgcaccagat gggtgagtgg gtcaccgatc ttctcgacca cactccggtc
     1741 cagttcggtg tcggcgccgc ggatgaccag ctcgatctcc ttgcccagct cgctgctgac
     1801 atcacgcacg acacgctgaa agcgactgaa ggtggtgccg atcggcacca tgcgcagttg
     1861 cagggcgctg tcgcgcaccg cctcgaccag ccgcatcact tcggcggtgg cttcgttcag
     1921 ggcagcatcg gccaccaccg aggcacgcag gttggcgccc gcgccagcaa tgaccaactc
     1981 gccgaccaga tcgatcagcc ggtcgagccg ttcggcgtcg acccggatgc tctgcgcctc
     2041 gcgggctttt ttctccttga tctgctgctg cctttccagc accgcctcga cgaccggctg
     2101 ctgcaccatc tgctgctcga ccagaatcgc gccgatcggc tgatgcggcg tgcctgcggc
     2161 actgctctgc tgctgcaagc gcagcccctc gtccagttca cgctgggtca ggatgccgct
     2221 tttgaccaga atctcaccga ggcgggcctc ctcttccggc agtgagcgga tcagctcgat
     2281 gtagtcgctg acctggctgt caggcggcag gatgcggacc gtgctgccat cgagcacgaa
     2341 ttcgaagacg ctctcgatct cctccttgct ggcaccgctg tgaaaatcga tctcgaaacc
     2401 gaggtagcag cactccggat cgatcgccgt cgcactgggc agactgtcgg tgagcgtcgc
     2461 cagatggatg atgtcaccca gcgtggccag atagcggatg aacgagaagg ggtccatgcc
     2521 gttgcgcaga cactcctcgg caaagcgcag cgacaggtgc cagttgccgc tgcgaagcgg
     2581 gccaccactg gtgtcgatgc gcgttgtccc ctgctcggcc ggggtcactg cctgaatgac
     2641 gccgctcacc aagggctgca accgtgccag cagggcagtg ccggcggcca gctgcggtcc
     2701 ttcatcggca tcgccgccgg ctgcgaccgc ctcgatcaac aggccgatgt ggtcatggca
     2761 tggcagcaag gtggcgatca gctcggcgct gacttcgatc cgtccgtcac gcagcgcatc
     2821 gagcacgctc tcgaccacat gggtgaactc gacaatgtgc gtcaaaccga acaggccggc
     2881 cgaccccttg atggtgtgga cggtgcgaaa tatcgcatcg atcgcctccg ccacctcctc
     2941 ggcctgctcc agaccgagca gtgcctcctc catctgttgc agcagttcgc gcgactcgac
     3001 aatgaaggtg tcgagcgcaa ccctcatctc atccttgaag ctcattggcc atcacctcat
     3061 gcacggtcgg gtcgatggga gagcaccagc gggtcatcga agaagttgac caggttggcc
     3121 agttcgaaca gttcgatgac ggccgggctg tgcagtacga agccgagccg cttgccgccg
     3181 cgccgcgcct gctgcttggc catgatcagc aactgcaggc cggtggtgtc gatctcatcg
     3241 accgcggcga gatcgatctc cagttcgtcg ccactgtcga gaaaggccag caaccgcgcc
     3301 ttctgttcgg cggcatggta gatggtcatc tcgccctcga atgccagtcg ttgcatcgtg
     3361 gtttcctcct ttctccgggc cattagggca gaatcagctt ggagacggcc tgcagcatct
     3421 gcgcgggctg aaacggcttg accacccagg ccttggctcc ggcggcctgg ccctcggcct
     3481 tcttgccctc ctgcgactcg gtggtcagca tgatcaccgg ggtgaatctg tagctgggca
     3541 gcttcttgac ctctttgacc aaggtgatgc catccatgtt gggcatgttg acgtcgctga
     3601 tgatcagatg gatcttgcgg ccatcgagct tcgtcaatgc atccttgcca tcacaggcct
     3661 ccaccacctc gtaaccggcc cccttgaggg caatgccgac cacttggcgc agcgaagcgg
     3721 agtcgtcgac gatcatgatg cttttggcca tggtggactc ctgttggcgc tcgttcagaa
     3781 aaaggtgatt tcgttcgact gcggtgctgc gaccgcccgc cccgcatggg cctggtgctg
     3841 gtcgaccatc gtgtagcgtc gggacgactc ttcgagccag cgcctggcat caagcggttc
     3901 ccgtgaaccc tgatcgcgct cgagccactg ctgcagcgcg tcgagatcgt cgcagacatg
     3961 ccccaggatc tgcttcaccc gatcctggaa ttgcagcgag accagcactt cgctgagctc
     4021 atcgccaatc atccggttct gcgcctgcaa cgcctgtgct gaatcgagca atgtcgaggc
     4081 ggtctgctcg aactgatcga gcacgcccgc gatgcgcgtt tcggcactgc tcaccctgtc
     4141 ggcatgttgc tgtgaagtgg tttcggagag cgtgagcgtg tgatggatgg caccactgac
     4201 cgccgccacc ttcgcgttga tgcgctcgac agtctcgccc gagcgcagcg acagcttgcg
     4261 cacctcatcg gccaccaccg aaaagccccg gcccgcttca ccggcacgcg ccgcttcgat
     4321 cgaggcgttg agcgccagca gttgggtctg ggtggcaatc ttgcccacct caccggtcat
     4381 ctgctgcaat tcaccggtga aggcggcaag ctgctggatg ctggctgcgg tggttttctg
     4441 ggcttgcaac accgcgtgca gggcgtccat caccacctgg agttcgccgc tgctgtgttg
     4501 cagcagtgcg ttgaggctgc tgtcctgatt ttcctgcgag gccaccacac cgccactgat
     4561 gtggtcatag agattggcga aacggctcga cagcgcggtg atggcctgct cggtctgcga
     4621 ctgcgccatg tcgatctgcc gcatccagat cggcaacgca tcgccacaga accgttcgaa
     4681 ctggagctgc tgctcttcgg tccggcttac ggcggtcggt tcggcgtcgg catcggcttg
     4741 cagcggcgcc acggttttgc ggtactggcg acccagcagt acacccacga cacagccagc
     4801 caggagcaac agcgtggatg aaagcagtgc caaagagtcg gccggatgca cggcccacac
     4861 cgtgagcgcg gcggccagac ccaacagcgc aggcgtcacg cggcatggca gcagaaatct
     4921 ggcctgctcc ttcgatttca ggttctccgc catcgaaaac atctccttga aaactgtcta
     4981 ttgctttgcc cgaactccct tcgctcttgg cggccgcctt gatggctttt tgcaggactc
     5041 acggataaac ctagaccaga ttggccgctt cgcaactttg tgttcagatc acccatgaaa
     5101 atgcaccacc acaccaacca accaaccgcg gagcatgggc aaatgaacga tgcaaacagc
     5161 ctgagcggcc tgaccgtcgc ccttgccgag agccgtgaac tcgacctgct ggcgcggatg
     5221 gtcgaggagc gtggtggccg ggtatggcgc tgtccgctgg tggcgatcgt cgatgcgccg
     5281 gacgaagcgg cggtgcaggc gtggatcgga cggatgatcg cccgcgagtt cgacctgttc
     5341 attctgttca ccggcgaggg gctgcgccgc ctgctggcgg cggccgggcg cagtcagcgc
     5401 gaggcggcac tgattgccgc cctggccgac accgagatcc tctgccgtgg ccccaagcca
     5461 gtgcgcgagc tgcgcaggct cggtctgaag ccgcagcatc aggccagcgc gccgaccacc
     5521 cagggggtga tcgagaccct cgaacagctc gacctgcgcg gacgccggat cacgctgcag
     5581 ctctatggca gcgaccccaa cccaccgctg atcgaccatc tggccgcacg cggcatcgtg
     5641 ccggtcacgg tggcgcccta cgcctatgca ccaaagagcg acgagatgca ggtggtggca
     5701 ctgatcgacg cgctggcgca gggtggcgtc gatgcgctgc tgttcaccag ccagccgcag
     5761 tatcgccgcc tggtcgaggt ggcacagcgc cacgaccgcg gtacacagtt ggcagctgga
     5821 ctggagcgaa tcaaggtggg agcgatcggg ccggtgatgg ccgatctgct gcatcaacag
     5881 ggggtgcggg tcgacttcat gcccgaggcg cgcttcttca tgaagccgct ggtgagcagt
     5941 ctggttcgcc acaccgaggg cggcgaaccg gcctgagtcg caaccctaca gttgcagaag
     6001 ctgcaaccgg tcgtcgtcgg cggcggccgc cggcgcggca gtggcggtga ggttgacgaa
     6061 atcgaacagc ctgacatcgg ccagttgcga cggggcgacg ttgcggatgg cggcaaagat
     6121 cacatcgagc cggcccggct gctgccgctc ccactgctgc agcatctcct tgaccttctg
     6181 ccgttgcaga ttctgctgcg agccgcagag gttgcaggga atgatcggga acgccttcag
     6241 ccgcgcatag gcggcgatgt cctcctcggt gcagaaggcc agcggccgga tcaccacatt
     6301 gcgcccatca tcgctgagca gcttcggcgg catcgccttc agcttgccgc catggaacag
     6361 gttgagaaac agcgtttcga tgatgtcgtc acggtggtgg ccgagcgcga tcttggtggc
     6421 gccgatctgc tgcgcaaagc tgtagagatt gccgcgccgc agccgcgagc agagaccgca
     6481 ggtggtcttg ccctcgggga cgatgctttt gacgatgctg taggtgtcgc gctccagaat
     6541 gtggaacgga acgccgagcc ggctcaggta gccgggcagc acctcctgcg gaaagccggg
     6601 ctgcttctgg tcgaggttga cggcgatcag ctcgaagtcg agcggcgcgt ggtcgcgcag
     6661 gttgagcaga atgtcgagca gggtgtagga gtccttgccg ccggagaggc agaccatcac
     6721 ccggtcgccc gcctcgatca tgttgtactc ttcgatggca cggccgacat tgcgccgcag
     6781 ccgcttctgc aacttgttga attcgaaacg gtcccgcttc gagagctgct cgatctccat
     6841 cgcctcgcct ctgctgtccg ccgcgctgac tccggagccc tccgcagcat cgatctgtcg
     6901 tgttgtcatg gtgtgctctc aaggttcggc cggcggacgc tcgccgaccg tggccgtcag
     6961 ctccatcggc tcactgccgc gcagcacgct gattctgacc tgctcgcccg gcctggcccg
     7021 ggcaatctgc tccatcgcca gccggccatt ccagctctcc cgaccgctca accgggtgat
     7081 gatgtcattc tctgcgatgc cggcctgctc ggccggactg ccggacacca ccgcggtgac
     7141 tcgcagaccc tcgatcctgg ccgtggcagc gccgggcagt tccacccgct ccacctcgat
     7201 gccgagccag ccgcgcacga cgcggccatg gtcgatcagt gcctgcatca cctggtaggc
     7261 caccgctgcc ggaatggcga agccgatgcc gagcgaaccg ccaccctgct ggctgtagac
     7321 cgcactgttg atacccacca gccgtccctc ggcattgacc agtgcaccgc cggagttgcc
     7381 gggatggatg gcggcatcgg tctggatgaa attctcaaag gtgctgaggc cgaggccagc
     7441 ccgcccggtg gcgccgatga tgccgaaggt cacgctctgc cccacgccga aggggttgcc
     7501 gatcgccagc accacgtcgc ccacctcgat ctgcttgccg tcggccagct cgatcggttg
     7561 caatccggtc aattcgatct tcagcagcgc cagatcggtc tcggggtcgg ccccgaccag
     7621 ccgggcagcg gcctcacgcc cgtcgcgcag cagcaccaga atgtcctcgg cattggccac
     7681 cacatggtgg ttggtgagaa tgtcgccctt gtggctgacg atgacgccag agccgagact
     7741 cgactgcatc cgctgtcgac gcgcctcgtc gttgccgcca tggattctgg ccagcagagg
     7801 atcatcgaga taggcgctgg tcgccgcaga gcgcttgcgg gtgtagacat tgaccaccgc
     7861 cggcgcggcg cgcttcaccg cctcggcgta ggaggcgacg accgcaggag gcgtctcacg
     7921 gtgcggttcg ctgacgggcg gcggtgcagc cggcggcgcg gcggatggcg ccggagcggg
     7981 cactggctcg ggcttgcgca accagtcagg cgaattgaac agcaacaaca ggcccaccag
     8041 cagccccagc agggctgcga tcagcagctc acgcggaccc cggttcatgc cagtccgaaa
     8101 atcgccgtgg cggtggtccg gatcgggtca gtcagcatcc gcttcaaggc tgatcgaggc
     8161 cgtagtcatc ggccagcagc cccttctcgt cagggtggcg tttgggtgcg tagtcgcgcg
     8221 gcggcgtcag tggcagctca tcgctgagtg gcagccgctc ggtcagccgc tccttctgaa
     8281 tccggttctg gatgacgatg cgttcgacag cctcgccgtt gcacagccgc tgggcgccgc
     8341 tggccagatg gtcgaagacg tcgcggtagc tctcggagag ccggccgacc agctcgctgg
     8401 tctgcacgaa gtggctgctc acctcatcgc ggtactggtc gaactcccgc tccagcgcgc
     8461 gggcatcgtg ctgggccttg gaccccccct ggcgcagtgc aagcagccaa cccattgcgc
     8521 cgcccagcag gacggcgacg acaaagatcg aaatggcttg ccagaactgc agcgtgatca
     8581 cgggcgactc cagagtgatg ccggccaggg agttccctgg acattgctag tgtagcaagc
     8641 ccaccaccgt gaagaaacgc gcccggtctt cggcaacgcg gatgacaccg acaggcgggg
     8701 agccccaaaa cgacaaagac gccagctggc gtctttgtgc gtggctcaac gtcagaacga
     8761 catcagtagg acttcggcag acccagcaga tgctgaccga tgtagttgag ggtcatctcg
     8821 cggttgatcg gcgccacctt gcccagccgc gcgccgacga agctgtgcag catgtccatg
     8881 ctctcggtga agccgtaacc cccatgcacc tgcaacgcgg catcggcggc gcggtagctc
     8941 gcctcggagg cggtgtactt ggcgatgttg gcggcagcac cggcattctc gccattgtcc
     9001 tgctgccagg cggccttcag cgtcatcagc caggccactt ccagcagcgc ggcggcatcg
     9061 gccagcggat gttgcagccc ctgataggca ccgatcggcc ggtcgaagat gacccgctgg
     9121 ttggcatact cgacaccacg cttcaggcac cagcgtccac cgccaacggc accggcggcg
     9181 acgacgatcc gctcggggtt gagggcatcg aacaggtagg caagcccctt gcccggctgc
     9241 ccgatcacat tctctttcgg cacccgcacg ttgttgaagt agacgaagta ctgcccttcg
     9301 gtgttctgca gcatggtgtc catcgggtcg aaggtgaggc ccggcgactt ggtgtcgacc
     9361 acgaacagcg tcagaccctc atattttttg tcttgcccca cctcggcata ggactgggtg
     9421 cgcgccacca ccagcacatg gtcggcctgg ttgatgccgc tgatgaacag cttggagccg
     9481 ttgagcacat agtcgccgcc atcgagcttg ccgagggtct ggatcttgaa ggtgttggtg
     9541 ccggcattgg gctcggtgat cgcgaaacag cagcgctttt caccgctcgc catcgccggc
     9601 agccagcgct gcttctgctc cggggtgccg tgcttggaga tgcagggcat cgcgatgccc
     9661 tggttgacga cgaagtagag cagcgccacg ccatggctgg cgagccgctc ctgcagcagg
     9721 gccagctcgg tcatgcccat gccggcgccg ccatactcct cgggacaggt gatgccgagg
     9781 tagcccatgc tgcccagctc attccacatc tcctgcggaa agcgcttgtg tttggcgcac
     9841 tcgagccaat atttgcgatc gaacttctgc gcgatcttgt cggcggagtc gacgatcagt
     9901 tgatgctctt gcgaaagggt gaaatccatg acgacatcct tgattgatta ttgtgaatcg
     9961 ttgaatttgc ccatgagcgc cgacgcgccc gtggaagctg gacaatacca cagcgttatg
    10021 atcgaacgaa tagcctgctg gcccgcctcg gctcggcggg cccaccgctt cagtggaatg
    10081 cctgccccag caccagggag agtacgatca gcgtgaccat cacgccaatc agtacggtca
    10141 cggtgatgcc cagccgtttg gcgacggtcg tatcttcatt attgtgcatg gcgcgatgcc
    10201 tcatgagtcg ggtggatcga tggcttgcaa gccgcagcgc ccactgttcc agtgtccgac
    10261 tccatatgca ttgatcagga tcaatctccg cgcactcttg cctgaacatc acccggatgc
    10321 tctaagatgg gcaggcgaac cggcacgaac gatcacaaca ataaaacgag gtgcaacatg
    10381 tatctgacag gcaaaagtgc attggtgacc ggcggcggac gcggcatcgg ccgcgccgtt
    10441 gcgctgcgca tggccgccga gggggccgcc gtggtggtga tggacccggg tgtcggccgc
    10501 gatggccgtg gcgccgaggg cgcgccggcc agcgaggtgg ccgaggagat ccgggccgcc
    10561 ggcggccagg ccgtgccctg ccacgagccg gtcgatgacc acgccgccgc cgaacgggcg
    10621 gtccatctcg ccgtcgaagc ctttggccgg ctcgacatcg tcgtcaactg cgccggcgtg
    10681 ctgcgtgagc ggatgatctg gaatctgacc gaagatgact gggacacggt gatccgcgtt
    10741 catctcaagg gccactacaa catcaaccac cacgccagca agctgatgcg cagccagcag
    10801 agcggccgca tcatcaactt ctcatcggat gcctggcgtg gcagcgtcgg ccaggtcaac
    10861 tatgccgccg ccaagggcgg catcgtcagc atgacgcgcg gactggcgcg tgaactgggg
    10921 cgctatggcg tcacctccaa tgcgatctgc ccgatggcgg caacgcggat gaccctcaac
    10981 gaaggggtga tcgccggcat gaagaagcgg ctggaggcgg ggctgatcac cccggagcgc
    11041 tttgccgcga tgcaggccat gcccgggccg gagtatgtcg cgcccttcgt ggcctatctg
    11101 gccagcgacc atgccgccca catcaatggg caggtgttcc atgtcgagaa ggggcgggtc
    11161 tccatctact cggagccggt cgaggagcgc gcgctctaca agcaggagaa cgatggccac
    11221 ttcacgctgg acgacctgat cgcccaggtg ccgcagaccc tgctgaatgg ctaccacaac
    11281 ccggcgcccc gcgaatcgca gccgtaaaca gggaatccaa ccacccaagg agagcacgat
    11341 ggcagagaaa caggtaggca ccaacatgcc cctcggcacc gtcgccgagg gccaggccat
    11401 ggtgggcaag cgcagccgca cccgcttcca caccatggat gtcaactggc cgatgatcag
    11461 gatctacgcc agcgcgatgg aagatggcaa cccctgctac tgggatgtcg actatgcccg
    11521 gcgtcgcttc ggcgacatcg tctcgccgcc cggcatgctg atggtctggg gcatggggct
    11581 cgactggcag cccgacgcgc cggtcgtcgc cgacgatccg ccgctgatga acatcccgct
    11641 gcccggcacc accatcatca acgtggtcac cgagaccgag ttcttccggc cgatccgggt
    11701 cggcgagcgg ctcaatgccc aggaagagct ggtcagcgtc agtgacgaga agagcacccg
    11761 gctcggcgtc ggctgcttca tccgcaccca gaccagttac cgcaaccagg caggcgagct
    11821 ggtcgccctt cacaccaatg acgtctaccg ctaccggccc cacgacaagg aggtgacccc
    11881 atgacccaca gcatcgccgg cgccttcggc aagaaatacc tctaccgcac ctggggcgag
    11941 gtcgaggtgg ggcaggcgct ccccgaggtg agaatgccga tcagctaccg ccgggtggtc
    12001 cagatgaccg tcgccacccg tgatttcttt cccggccacc acaaccgtga atatgcccgc
    12061 aaccaggggg tcgacgacat ctacctcaac accatgacct accatggcct gatcgaccgc
    12121 tatgtgctgg agtgggccgg acccgagtcg ttcctgcgca aacgctcgat gcgcatgggc
    12181 acctcgatct gcgccggcga cgaggtggtg atgaatggtc gggtgaccgc caaggagcgc
    12241 gagggcgagc atggcttggt ggtggtcgag atggtggtcg ccaaccgcgc cggcgtgccc
    12301 tgctgcccgg ccaccgtccg gctgtggatg cccgatcagt gagtgattcg cgcggtcagc
    12361 cgtgaccacc atcaccacca tcgacctgca acggctcggc ctgaagcctg acgatcgcct
    12421 gctcgatgtc ggctgcgggc tgggccgtca cacgctcgcc gcctaccgcc actgttcgct
    12481 gctggcggtg ggcgtcgacc tgtcgctggc cgacctgcaa caggcgcagc atcgctttgg
    12541 cgacttcgat caggactcac cccaccgcag cgccctctgg gcccaggccc acggcgcccg
    12601 gctgcccttt gccgatgcca gcttcgacaa ggtgatctgc tgcgaagtgc tggagcacct
    12661 cgaccggccg gaagcggtga tcagcgaact ggtgcgggtg ctcaagcccg acggcctgct
    12721 ggcggtttcg gtgcccagct actggccgga acggctctgc tggctgttga gccgcgacta
    12781 cccggccgac gtcggccatc tgcgcatctt ttgtggcgat gaactgccgc gcgccatcga
    12841 ggccagcggc ctgcgctgca tcgccgagca ccgcgagcat ggcctgcact caccctggtg
    12901 gtggctgcag gcgctgctct ggtctcgccg agagcagtcg tggctggtgc ggcgctacaa
    12961 ggattttctc gactgggcgc tgttccgctc gcccgcctgg ctgcggcggc tggagctgcg
    13021 gctcaatccg ctgctgggca agagccgggt cttctacttc acccggcgct gacccgcctc
    13081 ttcagccggc ctgacgcgac tccccgccat gccaggcatg gaactcggcc gtctcggcct
    13141 tgcccatgta acgggccagc gtcctgggtt cggtttgcag cagatcgacc atgatcagcc
    13201 catcgaggct gttgttgaac tggtcatcga cattgaacgc cagaatctgc ccgccgaact
    13261 tcagatattg ccgcagcagc accggaatgc ccttgccgtc cacctcgagt tcggcgatca
    13321 ggtcatccac cgcctcgacc gagttgaggt gtgcccgcgc actgtccacc gccttgccgg
    13381 cacgcgcccg gaatggcgag cgcggcttca ccagcttcgc cagcgtcggc tggctgttgt
    13441 ggatgcgcag aaaatcgacc agcagcttct gcgacagcgg ctgatagtcg ctgctgatgc
    13501 tgaccggccc gaacaggcgg cggtagcgcg gattgcggtg catgaaggtg gcgatgccct
    13561 tccacaacag cagcagcggc gcgaagctct tttgatactc ctgccgcacg aacgaacgcc
    13621 ccagctccag cgccggattg agctgatcga tcagccgccg tgaatagcgg aacagcgagt
    13681 ggctgtagag ccccgccacc ccccggctgg caacgatccg gtccgactca ccgaggcgat
    13741 aggcacccac cacctcgtcg gtgtcgcggt tccagatgaa aagatgcagg tagtaggcgt
    13801 cataggcatc gagatcgagc gcccgaccgg tgccctcgcc cgcggcgcga aagctcagct
    13861 cgcgcagccg gccgatctcg cgcagcagat ggggaatctg cccggcagtg gcgcaatgca
    13921 cctgcagcgc accgccatcg accagccgct gcgcttcggg cagcgcgtcg acctcctgcc
    13981 gcagcagcgc gcactcgacc ggctcgatca gggcgcaacc gctggtctga ccccgctcgc
    14041 gctggccgcc ggccggcttc gcactgttgc gcagcatgta ggtgcgcatc cgcagatagt
    14101 cgatcagcgc ctgatcctcc tcgatctgct tcaggcggct ccagggctgc accttgccga
    14161 tgcggatcgg gatggtggtg ttggtcttgt tgagcagctc tcgcggcagc atcacggtgc
    14221 gcagcagcgg atgcatcaga ccggcaagct ggaacagcag gctgttggcg ccatggaagt
    14281 agaccggctg cgccggcgcc tgcgccaact ggatcagccg gccgatgctg cggctccagg
    14341 tcgaatcctc gatgccgcga aacggcttga ggttggagac ctcgccggcc ggaaagacca
    14401 tcagcagccc gccctgcttc agccagcgca gcgcctcctt catcggaccg atgttgctgc
    14461 gcgccgcccc ggcaccacca aagggatcga cggcaatgaa cagatcacgc agctcgggaa
    14521 tgcgcttgag cacgaagttg gccatgatct tcacatcggg ccgcacctgg cgcagcagcg
    14581 ccgccatgat caccccctcg atggcaccga acgggtggtt ggccaccacc accagcggac
    14641 cactttgcgg aatgcgcgcc agctcctcgt cggccacctg atagtgaatg ttgaagagct
    14701 cgaacagctg ttgcagaaac agctgctgat cactgcaggg cgccagcgcg ctgtagagcc
    14761 ggtcgagctt cttcagcgac agaacacgct ccagcagtgc ctccatcggc cggctgatcg
    14821 cacggttggg aatcgcatcg agggtgccgg tgaggctgaa cggggtttcg gttgcggcgt
    14881 cgcgcatggt catcgcatcc ctttgaattg gaatggcgat gacggtaatc agcccggatg
    14941 acagtcagat aaaccgtggg tcaagattgt gtgacagcaa ggtgatctca gtttgcatac
    15001 aaaggtctca aggaaaccgt ggtctgtccc cgattctccc aaggaaaccg tggtctgtcc
    15061 ccgattctcc ctccgctgtc cccgattctc cctccgattc tccccttcag cactcagttg
    15121 ttcgtattcg tttcccatcg caacacctta acaaggtgtt gcacttgatt attgagccgg
    15181 cccttgcatc tcatagaaca gcgccgatca gactttcttg atgtactggg acttcaattg
    15241 catggatccg aatccttcaa tcttgcagtc gatgtcgtgg tcgccatcaa cgaggcgaat
    15301 gttcttgacc ttcgttccga ctttgacaac tgaagatgaa cccttaactt tgaggtcttt
    15361 gataacggtt accgaatctc cgtctttgag ttcgttgccg aaggcgtctt tccacacgcg
    15421 ccggtcttca acagcctctt ttgaagcagt actccattcg tggccgcatt ccgggcaaac
    15481 atactggcca ccatcttcat atgtgtaggc cgaattgcac ttcggacagc acggaaaatt
    15541 gctcatcatt attccttttc aaaaatctct tatagaaaaa gttgacacct gattgcgagc
    15601 cctaacaggc cgttgaaaaa cccgtcccct gtcatggcat gatgttgcca atattctgct
    15661 gatgcgagct tgagcgatgc gtggtcattc tgattcccaa gctgaccagg ggaattgggg
    15721 acagaccacg gtttcctgcc ttctgctctt cacaacaggt tgattcaaaa agattgattc
    15781 tcacgaaaca cacctccctg tcgccaggcg acacgccggg ttgccgttcg caaccgatat
    15841 gccgctgccc tcctcacaac ctcactcccc cgctacccac acttggaatc accgcagttg
    15901 aggcaggtga ggcagccatc cagcagcacc ttggccttga agtggcattt gtcgcacagt
    15961 tgcgcctggg ccggaaagtg gccgccctct tcggcagcga cgaggaggtg agcgaggaca
    16021 aggtgaaggg c
//
LOCUS       DAORCQ010000050        15879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2341_length_15879_cov_6.228387, whole genome shotgun sequence.
ACCESSION   DAORCQ010000050 DAORCQ010000000
VERSION     DAORCQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_15879_cov_6.228387"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   44..559
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=44..79
                     /rpt_unit_seq="gttcaaacacagacctgataaagaagggattaagac"
     gene            656..973
                     /locus_tag="PKI23_06795"
     CDS             656..973
                     /locus_tag="PKI23_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36681.1"
                     /translation="MKHEFELWRPGKDQPDGRITWDSDAGTLSGDLAEELEKLIDQAI
                     ADGVLVSHPMPTSYRITRPLHDAAQLGAIISLRWRTPELLADAMMRQGGDPAAKSSVE
                     LIY"
     gene            complement(1141..2664)
                     /gene="rmuC"
                     /locus_tag="PKI23_06800"
     CDS             complement(1141..2664)
                     /gene="rmuC"
                     /locus_tag="PKI23_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNN36682.1"
                     /translation="MTALTPIQIALPLLGLLLLWLGWRLLASRSRQTTTATELALIQE
                     RLQQTQARNGALEQALHQVSEREAQLQEQLRQETARRAAAEQSAEALPELLRERDRLL
                     QTLSERQATLAELGERLEQERRAAADKLALVDQAQLRLSDAFKALSAEALKSNNQAFI
                     ELAQQNLARFQEGARADLDSRHQAVDALIKPIGESLARFDGKLGELEQARISAYSALN
                     EQMRALVETHLPQLKSETASLVKALRQPNVRGRWGEIQLQRVVEMAGMLEHCDFEQQS
                     SRSSDEGRLRPDLIVRLPGGKQIVVDAKAPLAAYLEAMEASDDLSRATLLTDHARQVR
                     NHILALGKKQYWEQFNPAPEFVVLFLPGEMFFSAALQEDPSLIEYGVGERVIPATPTT
                     LIALLRAVAYGWRQQAQARNAQEISELGRELYKRLATLAEHWGKVGKGLNQAMEYYNK
                     ATATLESRVLVQARRFRELHAAPEGAEIALLEPLDHSARTLQAIEMQQEEPEAPASD"
     gene            2724..3353
                     /locus_tag="PKI23_06805"
     CDS             2724..3353
                     /locus_tag="PKI23_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN36683.1"
                     /translation="MKYAVIPVTPFQQNCTLFICEKSSKAVVIDPGGEVDRILQLVAQ
                     QGATVEKILVTHGHLDHASGVADLAERLGVPIEGPHEADRFWIEQIPKQGEMFGFKGR
                     SFEPDRWLADGDRVRFGEVTLEVLHCPGHTPGHVVFFAPEARLAQVGDVLFAGSIGRT
                     DFPLGDHQTLIDSITHKLWPLGDDVTFIPGHGPESTFGYERKTNPFVAD"
     gene            complement(3350..4153)
                     /gene="dapB"
                     /locus_tag="PKI23_06810"
     CDS             complement(3350..4153)
                     /gene="dapB"
                     /locus_tag="PKI23_06810"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNN36684.1"
                     /translation="MNRIAIAGAAGRMGKALIEASASTAQCRLTTVTVRPGSSLVGAD
                     AGELAGIGRLGLPISAGLARQLDRFDTLIDFTTPTATLDHLALCRQAGKRMVIGTTGF
                     TAAQRATIEAASREIAIVLAPNMSVGVNLCFKLLELAAQVLGDEVDVEVIEAHHRNKV
                     DAPSGTALRMGEVVAQALGRDLAQCALYGREGQTGVRDRQTIGFATVRAGDIVGDHTV
                     LFAGEGERVEITHKSSSRITYARGSLRAAQWLADRPHGLFDMQDVLGLR"
     gene            complement(4177..5310)
                     /gene="dnaJ"
                     /locus_tag="PKI23_06815"
     CDS             complement(4177..5310)
                     /gene="dnaJ"
                     /locus_tag="PKI23_06815"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNN36685.1"
                     /translation="MSKRDYYEVLGVARDASEQDLKKAYRRLAMKYHPDRNSDDADAE
                     QKFKEANEAFEVLSDADKRAAYDRFGHAGLEGAAGGSGFGGANFSDIFSDVFGDIFGG
                     GGGGGARRNRPQRGADLQYTLELNLEEAVRGHTVNIRVPILEECESCDGSGAKPGSTP
                     SNCTTCGGIGQVRMQQGIFSVQQTCPKCRGSGKMISNPCPKCRGEGRVEQQKTLSVKV
                     PPGVDNGDRIRLSGKGEAGLKGGPSGDLYVQIVVREHPIFERDGRNLYCTVPISFVDA
                     ALGGELEVPTLDGRVSLKIPPETQTNELFRVRGKGVAPVRGGAAGDLLCRVTIETPVN
                     LSEEQKTLLREFQRSLEQKGKNQSPKTHSWFDGVKKFFDDLTQ"
     gene            complement(5413..7338)
                     /gene="dnaK"
                     /locus_tag="PKI23_06820"
     CDS             complement(5413..7338)
                     /gene="dnaK"
                     /locus_tag="PKI23_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNN36686.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGSPKVIENSEGDRTTPSIIAYTS
                     DNEILVGQSAKRQAVTNPENTLYAVKRLIGRKFTDDVVQKDIKMVPYKIAKADNGDAW
                     VEVKGQKMAPPQISAQVLKKMKKAAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMDKKRGDSVVAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LSTNGDTFLGGEDFDLRVIEYLAEEFKKQSGIDLHNDPLALQRLKEAAEKAKIELSSS
                     QQTDINLPYITADASGPKHLVLKLTRAKLESLVDELVERTIEPVRIALKDAGRKPSEI
                     DEVILVGGQTRMPMVQTKVKEFFGREPRKDVNPDEAVALGAAIQAGVLSGDVKDVLLL
                     DVTPLSLGIETMGGVATVLIEKNSTIPTRKSQIFSTADDNQTAVTIHVVQGERKRAAD
                     NKSLGRFDLANIPPAPRGMPQIEVSFDIDANGILNVSAKDKATGKEQSIVIKASSGLS
                     DDEIASMVRDAESHAEEDRRFSELVAARNSGDGLIHATRKTLQEAADKVGSDEKQKIE
                     AAITALETAIKGDDVKAIESASEALSGLSAELAQKLYAEQAAAGGGEAQSAGAGRSSG
                     RDDDAVDAEFEEVKDDK"
     gene            complement(7440..8021)
                     /gene="grpE"
                     /locus_tag="PKI23_06825"
     CDS             complement(7440..8021)
                     /gene="grpE"
                     /locus_tag="PKI23_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNN36687.1"
                     /translation="MSDHDPTPVDDAAHGEAMAESHPASTAEQLVELQQKLAEAEAKV
                     SAAEERALRLQAEQQNLLRRSQQEIEKAHKFALEKFALELLPVVDNLERGLRAIDPQR
                     EDLQLFAEGMTLTLKSLLDALARFNIEPIDPAGQSFDPQLHQAVAMVELPGAAPGSVI
                     DVVQKGYSLNGRLLRAAMVAVAKGVSGSVDQRV"
     gene            complement(8106..9176)
                     /gene="hrcA"
                     /locus_tag="PKI23_06830"
     CDS             complement(8106..9176)
                     /gene="hrcA"
                     /locus_tag="PKI23_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNN36688.1"
                     /translation="MVRTRHTVASHGNADPALDTRERLLLRVLIERFIRDGEPVGSKS
                     LVSESGLCVSSATVRNTLARLEERGYLSSPHHSAGRVPTARGYRFFVDHLLHPTLPTA
                     EINQLWRQVDRSLSAPEMVESTSRLLADLTGMAGVVTLPRLNHVNLRRIEFLPLSDRR
                     VLVILVLHEREVQQRVIRVERDYSAMTLQQAANYLNLHFAGGSLQHIRDQLLLTMAQD
                     RAAMDQLMETVLEVASKTFAEPEEASADCLVLGEANLLSSEHLVTLDRARELFETLRH
                     KQEILHLLDRSLHTEGVRLLIGAESGLHSLQGCSVVTAPYCHQGRQVGVLGLIGPLRM
                     AYQQVIPMVSAAAQLLSTSLNR"
     gene            9245..10942
                     /gene="recN"
                     /locus_tag="PKI23_06835"
     CDS             9245..10942
                     /gene="recN"
                     /locus_tag="PKI23_06835"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNN36689.1"
                     /translation="MLTHLLISNYAVAERLEIDFQPGMTVITGETGAGKSILLDALAL
                     ALGDRADSASIREGAERAEIHVEFDLTHQPEVRAWLAEQELDNGEAHCLLRRTLLRDG
                     RSRAYLNGQPVPLTLLRDLGHQLIDIQGQHAHQRLLRREVQTALLDGYAMAQAEAEQV
                     AAFCQRWRQLQTQLRAREEENAATLARRELLEFQHTELEAAQPLPGEYAQLEAEQRLL
                     ANAESILQLGHQALGALRGDDDGNLLQGLRRLRQLLADWLRQGGTLPILTELVEGAAI
                     QLEEAATLLDRYLDELEVDEARLRAVDERLTTLHRLARKHQVPADELASLLASLDAQL
                     QRLHSVEGETAQLQAELEQIAQGYQTAATALGDKRRQAAPRFAAAVTARLRRLSLPDA
                     RFELQLTDSTSDLPRPEGQQSVEFLIVTNPGMPPRPLGKVASGGELSRIGLAVQVTAA
                     TTVATPTLVFDEVDVGIGGGVAEVVGQALHELAEHGQLLCVTHQPQVAAQADHHLQVV
                     KQVIDGRAATRLVELDREARTQEVARMLGGTQISARALEHARELVARGAQSSRAAES"
     gene            complement(10939..11358)
                     /gene="fur"
                     /locus_tag="PKI23_06840"
     CDS             complement(10939..11358)
                     /gene="fur"
                     /locus_tag="PKI23_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNN36690.1"
                     /translation="MSNQALRNAGLKVTLPRVKILQLLEVSNRRHMSAEDVYKALLAT
                     GEDVGLATVYRVLTQFEAAGLVIRHHFEGGNSVFELSDVDHHDHIVCIKCDRVEEFSD
                     AVVEARQEKISAEMGYELTGHSFYLYGICPQCRGAKP"
     gene            11469..11825
                     /gene="bamE"
                     /locus_tag="PKI23_06845"
     CDS             11469..11825
                     /gene="bamE"
                     /locus_tag="PKI23_06845"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNN36691.1"
                     /translation="MQKVKLLILALFPFILWGCSSTHDFRFPFAYRIDIQQGNLVKPE
                     AVAALKSGMTTQQVRYLLGSPMLTDPFHPDRWDYWYDLQAADGRTERQHLILYFTQGK
                     LARVERDNAPPPAKSE"
     gene            complement(11817..12137)
                     /locus_tag="PKI23_06850"
     CDS             complement(11817..12137)
                     /locus_tag="PKI23_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNN36692.1"
                     /translation="MAKGRIRVEVVHALAQRQRLIELEVAEDCTALQAVEQSLITLEF
                     PEIDLDHCRMGIFSQPIEAPAQHRLREADRVEIYRPLRIDAKSRRRQRAAIRKKQRAR
                     GRSL"
     gene            complement(12152..12595)
                     /locus_tag="PKI23_06855"
     CDS             complement(12152..12595)
                     /locus_tag="PKI23_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNN36693.1"
                     /translation="MTRIRRTVLLPYPVEKLYALINDIERYPEFMEGCVGAQLLCVEP
                     DTLVARLDLAKGPLRQSFTTRNHLHPPHSIRMELEAGPFSQFAGQWQLDALGTQGCKV
                     SLDLEFELASKLISLAAGKLFQSVADQMVDAICLRAHTLLGAARP"
     gene            12700..13182
                     /gene="smpB"
                     /locus_tag="PKI23_06860"
     CDS             12700..13182
                     /gene="smpB"
                     /locus_tag="PKI23_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNN36694.1"
                     /translation="MSKPAKTAPQGGTIALNRRARHDYALEERFEAGIALEGWEVKSL
                     RAGKAQLVDAYVLLKEGEAFLLGALITPLGTASTHITPDPTRTRKLLLNRRELNQLIG
                     AVTQKGYTCIPTALYWKGNHVKCEVALAKGKKLYDKRASEKERDWAREKERVLRHTTR
                     "
     gene            complement(13197..14774)
                     /locus_tag="PKI23_06865"
     CDS             complement(13197..14774)
                     /locus_tag="PKI23_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNN36695.1"
                     /translation="MRNNQPVSQHERLFEPSQQLISCTDLGGRITYVNDDFVALSGFT
                     REELIGQPHNVIRHPDMPPAAFQQMWAKLKRGEPWLGVVKNRCKNGDHYWVLAYVTPI
                     VQDGQAVGYQSVRVLPQRDYIERAERLYATLRDKAPTVTLRSPILGLPARVAALILTT
                     AGLAAVPGMVPDWSGGFMSLWWWGAAAMVGGFGIHWTTARLRKVAEAERKRYPNLLTA
                     QVLFGAGDELAQLELGQQYRDAHIRTVLVRVSDAAQKISALASQTAQIAVATEAEAAR
                     QDEELSQAATAMNEVSASTHEVAHHARSTATASEEVNGQVNQGRQEMAAMVGFSRQLD
                     GEIESASGTVESLRRRSEEIGSVLQVIGEITEQTNLLALNAAIEAARAGDVGRGFAVV
                     ADEVRTLAQRTASATEQVRQTITALQAEAEQAANAMADARKVAREGFERAEQTDDTLA
                     AIATAMERISEMNRQIAQGCREQGEAAESILRGIVNVSDVSKSVREQSASSTEVSRDL
                     TQQVDQILGAVIQQFAE"
     gene            14996..15356
                     /gene="ssrA"
                     /locus_tag="PKI23_06870"
     tmRNA           14996..15356
                     /gene="ssrA"
                     /locus_tag="PKI23_06870"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(15643..15876)
                     /locus_tag="PKI23_06875"
     CDS             complement(15643..15876)
                     /locus_tag="PKI23_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNN36696.1"
                     /translation="MDSCQLIRKETKMDVVHLNQKQLAARWHLSEACLERWRSEGIGP
                     KFLKLCGRVLYRQVDIESCEESCLQVSTSASPR"
BASE COUNT         2698 a         5127 c         5067 g         2987 t
ORIGIN      
        1 ttaagaccgc agttgcggca atctgttttt tggtggccgg ccggttcaaa cacagacctg
       61 ataaagaagg gattaagact ccgcaatcca tgccgatatg ccgactattc tcgttcaaac
      121 acagacctga taaagaaggg attaagactt aggtcattcg ggctttgatg gcgaccaatc
      181 acgttcaaac acagacctga taaagaaggg attaagaccg aagaccggcg gttttgccga
      241 tgcatcggcg ttcaaacaca gacctgataa agaagggatt aagaccttcg ttcgccttgt
      301 atgccgggtt tgaaatggtt caaacacaga cctgataaag aagggattaa gacccaccgg
      361 caatctgttt gaggtacgca acgctggttc aaacacagac ctgataaaga agggattaag
      421 actgcacgcg ataaaaaggt tgggacggtt gggagttcaa acacagacct gataaagaag
      481 ggattaagac cgattgccgc gatgtccccg tcggaccacc gatgttcaaa cacagacctg
      541 ataaagaagg gattaagacc tgtcgcgatt acgattttta aatcatgaaa cgcgcaagtg
      601 ctgagctgtc gattgcgttg tgcacacatt cgggccatga cggaggcaaa aggaaatgaa
      661 gcatgaattc gagctctggc gacccggcaa agaccagccc gatgggcgga tcacctggga
      721 cagtgacgcc ggcacactga gtggtgatct ggccgaggag ctggaaaagc tgatcgatca
      781 ggccatcgcc gatggggtgc tggtgagcca cccgatgccg accagctatc gcatcacgcg
      841 accgctgcac gacgcggctc aactgggtgc gatcatcagt ctgcgctggc gcacccccga
      901 actgctggcc gatgcgatga tgcggcaggg tggcgatccc gctgcaaaga gcagcgtcga
      961 gctgatttac tgatgaccgc ccgagggttt gcagtcgcgc gatgctgtga gccgaccccg
     1021 accggcatcg tgactcgcaa cgggaatcga acctcttcat cctgtcgagg gggcgccgca
     1081 tgaggcggcg gcaggcatcg gccatccgcg cgccggttcc ccggccggcc ggtgaccggt
     1141 tcaatcgctg gccggtgctt cgggctcttc ttgctgcatt tcgattgcct gcagggtgcg
     1201 ggcgctgtgg tcgagcggtt cgagcagggc gatctcggcc ccttcgggcg ctgcgtgcag
     1261 ttcgcgaaag cggcgcgcct gcaccagcac gcgcgactcc agcgtggcag tggccttgtt
     1321 gtagtactcc attgcctggt tgagcccctt gccgaccttg ccccagtgct cggccagggt
     1381 ggcgagccgc ttgtagagct cgcgtcccaa ctcgctgatc tcctgggcat tgcgtgcctg
     1441 ggcctgctgc cgccagccgt aggcgaccgc gcgcagcagc gcgatcaggg tggtgggggt
     1501 ggccggaatg acccgctcgc cgacaccata ttcgatcagt gacgggtctt cctgcagtgc
     1561 ggcagagaag aacatctcgc cgggcaggaa cagcacgacg aactcgggtg ccgggttgaa
     1621 ctgttcccag tactgcttct tgccgagggc gaggatgtgg ttgcgcacct gccgggcatg
     1681 gtcggtcagc agggtggcgc gactgaggtc gtcgctggct tccatcgcct ccaggtaggc
     1741 ggccagcggc gccttggcat cgacgacgat ctgcttgccg ccgggcaggc gaacgatcag
     1801 gtcaggccgc agccgtcctt cgtcgctgct gcggctgctc tgctgctcga agtcgcaatg
     1861 ttcgagcatc ccggccatct ccaccacccg ctgcaactgg atttcgcccc agcggccacg
     1921 cacgttgggc tggcgcagcg ccttgaccag gctggcggtc tcgctcttga gctgcggcag
     1981 gtgggtttcg accagcgccc gcatctgctc gttgagggcg ctgtaggcac tgatgcgcgc
     2041 ctgctcaagt tcgccgagct tgccgtcgaa ccgggccagt gactcgccaa tcggtttgat
     2101 cagcgcatcg acggcctggt ggcggctgtc gaggtcggcg cgcgcgccct cctgaaagcg
     2161 ggccaggttc tgttgcgcca gttcgatgaa ggcctggttg ttgcttttga gcgcctcggc
     2221 ggagagtgcc ttgaaggcat cgctgagccg cagttgcgcc tggtcgacca atgccagctt
     2281 gtcggcggca gcacgacgct cctgctccag ccgttcgccc agttcggcca gggtggcctg
     2341 ccgttcgctc agcgtctgga gcagccggtc gcgctcgcgc agcagttcgg gcagcgcctc
     2401 ggcgctctgt tcggcggcag cgcggcgggc agtctcttgc cgcaactgct cctgcaactg
     2461 ggcttcgcgc tcgctgacct gatgcagcgc ctgctccagc gcgccgttgc gcgcctgcgt
     2521 ctgctgcaac cgctcctgga tcagcgccag ttcggtggcg gtggtcgtct gccggctgcg
     2581 gctcgccagc aggcgccatc ccagccagag caggagcagt ccgagcaggg gcagtgcaat
     2641 ctggatgggc gtcagtgctg tcatggtata gtccaaattc aggtcatcgc cgcggcgatc
     2701 gttcggcagt cagggtctca atcatgaaat atgcggtcat tccggtcacc ccctttcaac
     2761 agaactgcac gcttttcatc tgcgaaaaga gcagcaaggc agtggtgatc gaccccggtg
     2821 gcgaggttga ccgcatcctg caactggtgg cgcagcaggg cgccacggtc gagaagattc
     2881 tggtgaccca tggccacctc gaccatgcca gtggcgtggc cgatctggcc gaacggctcg
     2941 gggtgccgat cgagggaccg catgaggcgg accgcttctg gatcgagcag attcccaagc
     3001 agggcgagat gttcggcttc aaggggcgca gcttcgagcc agaccgctgg ctggccgatg
     3061 gtgaccgggt ccgctttggc gaggtgaccc tcgaggtgct gcactgcccg ggccacacgc
     3121 cgggccatgt ggtcttcttc gcgcccgagg cgcggctggc ccaggtgggg gatgtgctct
     3181 tcgccggctc gatcggccgc accgactttc cgctcggtga ccaccagacg ctgatcgact
     3241 cgatcaccca caagctctgg cctctgggcg atgatgtcac cttcattccg gggcatggtc
     3301 cggagtcgac cttcggctac gagcgcaaga ccaacccctt cgttgccgat tagcgcagcc
     3361 ccagcacatc ctgcatgtcg aacagcccgt gcggccggtc ggccagccac tgggcggcgc
     3421 gcagcgagcc gcgcgcatag gtgatgcggc tgctcgattt gtgggtgatc tcgacccgct
     3481 cgccctcgcc ggcgaacagc acggtgtggt cgccgacgat gtcgccggcg cgcaccgtgg
     3541 caaagccgat ggtctggcgg tcacgcacgc cggtctgccc ttcacggcca tagagcgcgc
     3601 actgcgccag atcgcggccg agcgcctggg ccaccacttc gcccatccgc agcgccgtgc
     3661 ccgagggggc gtcgaccttg ttgcggtggt gcgcctcgat cacttcgaca tcgacctcat
     3721 cacccagcac ctgggcggcc agttcgagca gcttgaagca gaggttgacg ccgacgctca
     3781 tgttgggggc cagcacgatg gcgatctcac ggctggcggc ttcgatggtg gcgcgctgcg
     3841 cggcggtgaa gccggtggtg ccgatcacca tgcgcttgcc tgcctgccga cagagcgcca
     3901 gatggtcgag cgtggccgtg ggggtggtga agtcgatcag ggtgtcgaac cgatcgagct
     3961 gccgtgccag cccggcgctg atcggcaggc cgaggcggcc gatgccggcc agctcgccgg
     4021 cgtcggcacc gaccagcgag ctgccgggtc gcaccgtcac cgtggtgagc cggcactgtg
     4081 ccgtgctggc ggaggcctcg atcagcgcct tgcccatgcg tccggcggcg ccggcaatgg
     4141 cgatgcggtt catctgtgct tgcgtctgca acggggtcat tgggtcaggt cgtcgaagaa
     4201 cttcttgacg ccgtcgaacc aggagtgggt ctttggcgac tggttcttgc ccttctgctc
     4261 caggctgcgc tggaattcgc gcagcagcgt cttctgctct tcgctgaggt tgaccggggt
     4321 ctcgatggtg acccggcaca gcaggtcgcc ggcggcaccg ccacgcaccg gcgccacccc
     4381 cttgccccgc acgcggaaca gctcgttggt ctgggtttcg ggcggaattt tcagtgacac
     4441 ccggccatcg agcgtcggca cctccagctc gccacccagg gcggcatcga cgaaggagat
     4501 cggcacggtg cagtagaggt tgcggccgtc acgctcgaag atcggatgct cgcgcaccac
     4561 gatctgcaca tagaggtcgc cgctggggcc gcccttgagc cctgcctcgc ccttgccgga
     4621 gaggcggatg cggtcgccat tgtcgacacc gggcggcacc ttgaccgaca gggtcttctg
     4681 ctgctcgacc cggccctcgc cccggcactt ggggcagggg ttgctgatca tcttgccgct
     4741 gccccggcac ttcgggcagg tctgctgcac cgagaagatg ccctgctgca tccgcacctg
     4801 accgatgccg ccgcaggtgg tgcagttcga gggcgtcgag cccggtttgg cgccggagcc
     4861 atcgcagctt tcgcactctt cgagaatcgg cacacggatg ttgacggtgt ggccgcgcac
     4921 cgcctcttcg aggttcagct cgagcgtgta ctgcaagtcg gcaccgcgct gcggccggtt
     4981 gcggcgtgca ccgccgccgc ccccgccacc gaagatgtcg ccgaagacat cgctgaagat
     5041 gtcgctgaag ttggcgccac caaagccgct gccaccggcg gcaccttcga gcccggcatg
     5101 gccgaagcgg tcgtaggcgg cccgtttgtc ggcatcggag agcacctcga aggcttcatt
     5161 ggcctccttg aacttctgct ccgcatcagc atcgtcgctg ttgcggtcag ggtggtactt
     5221 catcgccagg cgacggtagg ccttcttcag atcctgctcg gaggcatcgc gggcgacacc
     5281 cagcacttcg taataatcgc gcttcgacat ggctctaggg gttgctctgt ttcgcagtca
     5341 gtcaaacaaa aaggacgcaa agcgggcttt gcgtccagag cgtgcaccgc cggtgcggtg
     5401 aatcagaggg ccttacttgt catccttgac ctcttcgaac tcggcatcga cggcgtcatc
     5461 gtcgcggccg ctgctgcggc cggcgccggc cgactgcgct tcgccgccgc ccgccgcagc
     5521 ctgctcggcg tagagcttct gcgccagttc ggcggagagt ccgctgagcg cctcgctggc
     5581 gctctcgatg gccttgacgt catcgccctt gatggcggtt tcgagtgcgg tgatggcggc
     5641 ctcgatcttc tgcttctcgt cgctgccgac cttgtcggcg gcctcctgca gcgtcttgcg
     5701 ggtggcgtgg atcaggccat cgccactgtt gcgcgcggcg accagctcgc tgaagcggcg
     5761 atcctcctcg gcgtgggatt cggcgtcgcg caccatgctg gcaatctcgt catccgacag
     5821 gccactcgat gccttgatga cgatcgactg ctccttgccg gtcgccttgt ccttggccga
     5881 cacgttgagg atgccgttgg catcgatgtc gaagctcacc tcgatctgtg gcatgccgcg
     5941 cggtgccggc ggaatgttgg ccagatcgaa ccggcccagc gacttgttgt cggcggcacg
     6001 cttgcgctcg ccctgcacga catggatggt gactgcggtc tggttgtcgt cggcggtgct
     6061 gaagatctgc gacttgcggg tcgggatggt gctgttcttt tcgatcagca cggtggcgac
     6121 gccgcccatg gtctcgatgc cgagcgacag cggggtgacg tcgagcagca gcacatcctt
     6181 cacgtcaccc gacagcacgc cggcctgaat ggctgcgccc agcgccaccg cctcgtcagg
     6241 gttgacatcc ttgcgcggct cgcgaccgaa gaactccttc accttggtct gcaccatcgg
     6301 catgcgggtc tggccgccga ccaggatcac ctcgtcgatt tcgctcggct tgcggccggc
     6361 atccttcagt gcgatgcgca ccggctcgat ggtgcgctcg accagttcgt cgaccagcga
     6421 ctccagcttg gcgcgggtca gcttcagcac caggtgcttg ggaccactgg catcggcggt
     6481 gatgtagggc aggttgatgt cggtctgctg gctgctcgac agctcgatct tggccttttc
     6541 ggccgcctcc ttgagccgtt gcagcgccag cggatcgttg tgcaggtcga tgccgctctg
     6601 cttcttgaac tcctcggcca gatattcgat gacacgcagg tcgaagtcct cgccacccag
     6661 gaaggtgtcg ccattggtcg agagcacctc gaactgatgt tcgccgtcga cctcggcgat
     6721 ctcgatgatc gagatgtcga aggtgccgcc gccgaggtca tagaccgcca ccaccgagtc
     6781 gccccgcttc ttgtccatgc cgtaggccag ggccgcggcg gtcggctcgt tgatgatccg
     6841 cttgacgtcg agcccggcga tgcgcccggc atccttggtc gcctgccgct gcgagtcgtt
     6901 gaagtaggcc ggcacggtga tcactgcctc ggtgacctct tcgccaaggt aatcctcggc
     6961 ggctttcttc atctttttca gcacctgtgc cgagatctgc ggcggcgcca tcttctgccc
     7021 cttcacctcg acccaggcat cgccgttgtc ggccttggcg atcttgtagg gcaccatctt
     7081 gatgtccttc tgcacgacat cgtcggtgaa cttgcggccg atcagccgct tgaccgcgta
     7141 gagggtgttc tcggggttgg tgaccgcctg ccgcttggcc gactggccga ccagaatctc
     7201 gttgtcgctg gtgtaggcga tgatcgaggg ggtggtgcgg tcgccttcgg agttctcgat
     7261 caccttggga gaaccgccct ccatcactgc cacgcaggag ttggtggtgc ccaggtcgat
     7321 gccaatgatt tttcccattc gtctctctcc gctttgctgg atccgggcct tgcttcagcc
     7381 cgctctggat gttgtgctgg atattggcgt cattgcgcca ttttcaaggc cggatcggtt
     7441 cagacgcgct gatcgaccga gccgctgacc cccttggcga ccgccaccat ggcggcccgc
     7501 agcagccggc cattcagact gtagcccttc tggaccacat cgatgacgct gccaggggcg
     7561 gcgccgggca gctcgaccat cgccaccgcc tgatgcagtt gcgggtcgaa cgactggccg
     7621 gccgggtcga tcggttcgat gttgaagcgg gccagggcat cgagcagtga cttgagggtc
     7681 agtgtcatgc cctcggcgaa gagttgcaga tcttcccgct gcgggtcgat ggcacgcagg
     7741 ccgcgctcga ggttgtcgac caccggcagc agttcgagcg cgaacttctc cagcgcgaac
     7801 ttgtgggcct tctcgatctc ctgctggctg cggcgcagca ggttctgctg ctcggcctgc
     7861 aggcgcagcg cgcgctcttc ggccgcgctg accttggctt cggcctcggc cagcttctgc
     7921 tgcaattcga cgagctgttc tgccgtgctg gccggatgtg attctgccat cgcttcgccg
     7981 tgggcggcgt cgtcaacggg tgtcggatca tggtcggaca tgccaagggc tccctgaaat
     8041 tttcacgaat acgaaagcgc gccatcaccc tcacgggcga ggcagggccg atgtggggct
     8101 attcttcagc gattcaagga ggtggagagc aattgggccg cagcactgac catcgggatc
     8161 acctgctggt acgccatgcg cagcggaccg atgaggccca gcacgcccac ctgccggccc
     8221 tggtggcagt agggcgcggt gaccacgctg cacccctgca gtgagtgcag ccccgactcg
     8281 gcaccgatga ggaggcggac gccctcggtg tgcagcgagc ggtcgagcag atggaggatc
     8341 tcctgcttgt gccgcagggt ttcaaacagc tcgcgggccc gatccagcgt gaccagatgc
     8401 tcgctgctga gcaggttggc ttcgcccagc accagacagt cggccgatgc ctcttcaggc
     8461 tcggcgaagg ttttgctggc cacctccagc actgtctcca tcagctggtc catcgccgca
     8521 cggtcctgcg ccatcgtcag caacaactga tcgcgaatgt gctgcagcga gccgccggcg
     8581 aagtggaggt tgaggtagtt ggcggcctgc tgcaacgtca tggcgctgta gtcgcgctcg
     8641 acccggatca cccgctgctg cacctcgcgc tcatgcagca ccagaatcac cagtacacgg
     8701 cggtcgctga ggggcaggaa ctcgatgcgc cgcagattga catggttgag ccggggcagc
     8761 gtgacaacac cggccatgcc ggtcaggtcg gccagcagcc gcgaggtcga ttcgaccatc
     8821 tccggggccg acagcgagcg gtcgacctga cgccagagct ggttgatctc ggcggtcggc
     8881 agcgtcggat gcagcagatg gtcgacaaag aaccgatagc cgcgcgcggt cggcacccgg
     8941 ccggccgagt ggtgcggcga actgagatag ccccgctcct ccagcctggc cagcgtgttg
     9001 cgcacggtgg ccgaactgac gcagaggccg ctttcgctga ccagactttt cgagccgact
     9061 ggctcgccat cacgaatgaa gcgctcgatc agaacgcgca gcaacagacg ctcgcgggtg
     9121 tcgagtgcag ggtctgcatt accgtgactt gcgacggtgt gacgggtgcg caccatggtc
     9181 ttgggtcctt gatccctctt acaatcaccc cggcggggca acagcacaga tattcatcaa
     9241 ctatatgctg acacatttgc tgatttcaaa ttatgccgtg gcagaacggc tcgaaatcga
     9301 ctttcagccg ggcatgaccg tgatcaccgg cgagaccggt gccggcaagt cgattctgct
     9361 cgatgcgctg gcgctggcgc tcggtgaccg tgccgacagc gcctcgatcc gcgaaggggc
     9421 cgagcgtgcc gagatccacg tcgaattcga cctgacccac cagcccgagg tgcgcgcctg
     9481 gctggccgaa caggagctcg acaacggcga ggcacactgc ctgctgcgtc gcacgctgct
     9541 gcgcgatggc cgctcccgcg cctacctgaa tggacagccg gtgccactca ccctgctgcg
     9601 cgatctgggg catcagttga tcgacatcca gggtcagcat gcccatcaac gactgctgcg
     9661 ccgcgaagtg cagaccgcgc tgctcgatgg ttacgccatg gcccaggcgg aggcggaaca
     9721 ggtggccgca ttctgccagc gctggcggca gctgcagacg cagcttcgag cgcgcgagga
     9781 ggaaaatgcc gccacgctgg cgcggcgcga attgctggag ttccagcaca ccgaactcga
     9841 agcggcccag ccgctgcccg gcgaatatgc ccagctcgaa gccgagcagc ggctgctggc
     9901 caatgccgaa tcgatcctgc agctgggcca tcaggcgctg ggcgcgctgc gcggcgatga
     9961 cgacggcaac ctgctgcagg ggctgcgccg tctgcgtcag ttgctggccg actggctgcg
    10021 gcagggcggc acgctgccga tcctgaccga gctggtggaa ggcgccgcga tccagttgga
    10081 ggaggccgcc acgctgctcg accgttacct cgacgaactc gaggtggacg aagcccggct
    10141 gcgtgccgtc gacgagcggc tcaccaccct gcatcggctg gcgcgcaagc atcaggttcc
    10201 ggccgatgaa ctggcatcgc tgctggcgtc gctcgatgca cagttgcagc ggctgcactc
    10261 ggtcgagggt gaaaccgcgc agttgcaggc cgagctggag cagatcgcgc aggggtatca
    10321 gaccgccgcc accgccctgg gcgacaagcg gcgccaggcc gccccccgct tcgcggcggc
    10381 ggtgaccgcc cggctgcggc ggctcagcct gcccgatgcc cgcttcgaac tgcaactgac
    10441 cgacagcacc agcgacctgc cgcgtccaga gggtcagcag agcgtcgaat tcctgatcgt
    10501 caccaacccg ggcatgccgc cgcgaccact cggcaaggtg gcttcgggag gtgagctttc
    10561 ccgcatcggt ctggcggtgc aggtgaccgc cgccaccacg gtcgccacgc ccaccctggt
    10621 gttcgatgag gtggatgtcg gcattggcgg tggcgtggcc gaagtggtcg gccaggcact
    10681 gcatgagctg gccgaacacg ggcaactgct ctgcgtgacc catcagccgc aggtggcggc
    10741 ccaggccgac caccacctgc aggtggtcaa gcaggtgatc gatggccgtg ccgccacccg
    10801 gctggtggag ctggatcgag aggcgcgcac ccaggaggtg gcgcgaatgc tgggcgggac
    10861 acagatcagc gcacgggcgc tcgaacatgc ccgcgagctg gtggccaggg gcgcgcagtc
    10921 atcccgggct gccgaaagtt agggcttggc gccccggcac tgggggcaga tgccgtagag
    10981 atagaagctg tgaccagtca gctcatagcc catctcggcg gagattttct cctgccgcgc
    11041 ttcgaccacg gcatcggaaa actcctcgac cctgtcgcac ttgatgcaga cgatgtggtc
    11101 gtggtggtcg acatcggaga gttcgaagac cgagtttccg ccctcgaagt ggtggcggat
    11161 gaccagcccc gccgcctcga actgggtcag cacccggtag accgtcgcca gaccgacatc
    11221 ctcgccggtc gccagcagcg ccttgtagac atcctcggcg ctcatgtgcc gccgattcga
    11281 tacttcgagc agctgcagaa tcttgacacg cggcagcgtg accttgaggc cggcgtttct
    11341 cagcgcctgg ttggacatgg tgtgatcaac ccgtgctcgt gtagggggct tggaattagg
    11401 gtatgatcgc tggccgtgtc tgcctgcggt gacaccccgc tcaccaagat aatggaatcg
    11461 aaccctcgat gcaaaaggtt aagctgctga ttttggccct gtttccgttt attttgtggg
    11521 gctgttcgag cacgcacgac ttccgttttc cgtttgccta ccgcatcgac atccagcagg
    11581 gcaacctggt caagcccgag gcggtggccg cgctcaagag cggcatgacc acccagcagg
    11641 tgcgctatct gctcggctcg ccgatgctga ccgatccctt ccaccccgac cgctgggact
    11701 actggtacga cctgcaggcc gccgatgggc gcaccgaacg ccagcatctg atcctctact
    11761 tcacccaggg caagctggcg cgggtcgagc gcgacaacgc accgccgccg gccaagtcag
    11821 agtgagcgtc cgcgagcgcg ctgttttttc ctgatcgcgg cgcgctggcg ccgccggctc
    11881 ttggcatcga tccgcaatgg ccggtagatc tccacccggt ctgcctctcg cagccggtgc
    11941 tgcgccggtg cttcgatcgg ctgcgaaaag atccccattc ggcagtgatc cagatcgatc
    12001 tcgggaaact ccagcgtgat gagcgactgc tcgaccgcct gcagcgccgt gcagtcttcg
    12061 gccacctcca gttcgatcag ccgctgtcgc tgcgccagtg catggaccac ctcgaccctg
    12121 atcctgccct ttgccatcgc cacctcgtgc ctcagggtct ggctgcgccc agcagcgtgt
    12181 gcgcccgcag gcagatggca tcgaccatct gatcggccac actctggaac agtttgccgg
    12241 cggccagact gatcagcttg ctggccagtt cgaactccag atcgagactc accttgcagc
    12301 cctgcgtccc cagggcatcg agttgccact ggccggcgaa ctggctgaat ggccccgcct
    12361 ccagctccat ccggatcgag tggggggggt gcaggtgatt gcgcgtggtg aagctctgcc
    12421 gcagcggtcc cttggccaga tcgagccggg ccaccagtgt gtctggctcg acgcagagca
    12481 gttgcgcacc gacgcagccc tccatgaact cgggatagcg ttcgatgtcg ttgatcagcg
    12541 catagagttt ctcgaccgga tagggcagca ggacagtgcg gcggatgcgg gtcatcgatc
    12601 aactctggcc agaggtaaac tgcttgaagg ggcgaccatt ttgcggcgcc gttcccggca
    12661 gctcaagcat cttcgtcgcc gtcgccctat aatggcacga tgagcaaacc cgccaagacc
    12721 gccccgcagg gcggcaccat tgccctcaac cgccgcgccc ggcacgacta cgcgctcgaa
    12781 gagcgcttcg aggccggcat tgcgctggaa ggctgggagg tcaagagtct gcgtgccggc
    12841 aaggcgcaac tggtcgatgc ctatgtgctg ctgaaggagg gcgaggcctt tctgctcggc
    12901 gcactgatca cgccgctcgg taccgcttcg acccacatca cgcccgaccc cacccgcacc
    12961 cgcaagctgc tgctcaaccg tcgcgagctg aaccagttga tcggtgccgt cacccagaag
    13021 ggctacacct gcatccccac cgcgctctac tggaagggca accacgtcaa gtgcgaagtg
    13081 gccctggcca agggcaagaa gctgtacgac aagcgcgcca gcgaaaagga gcgtgactgg
    13141 gcgcgggaga aagagcgggt gctgcgtcac accacccgct gatcagccgc gcggcctcac
    13201 tctgcgaact gctggatgac cgcacccaga atctgatcga cctgttgtgt caggtctcgg
    13261 ctcacttcgg tgctggaggc agactgctcc cgcaccgatt tcgagacatc gctgacgttg
    13321 acgattccac gcaggatcga ctccgccgcc tcaccctgct cacggcagcc ctgcgcgatc
    13381 tgcctgttca tctcggagat ccgttccatg gcggtggcga tcgccgccag agtgtcatcg
    13441 gtctgctcgg cacgctcgaa accctcccgt gccaccttgc gcgcatccgc catcgcattg
    13501 gcggcctgct cggcttccgc ctgcaacgca gtgatggtct gtcgcacctg ctcggtggcg
    13561 ctggcggtgc gctgtgccag cgtgcgcacc tcgtcggcca ccaccgcaaa gccgcggccg
    13621 acatcgccag cccgggccgc ctcgatggcg gcattgaggg cgagcaggtt ggtctgctcg
    13681 gtgatctcgc caatcacttg cagcaccgag ccaatctcct cgctgcgccg ccgcagagac
    13741 tcgaccgtgc cggaagccga ctcgatctcg ccatcgagct ggcgactgaa gcccaccatc
    13801 gccgccatct cctgccggcc ctggttgacc tggccattca cctcttcgct ggcggtcgcg
    13861 gtgctgcgcg catggtgcgc cacctcatgg gtgctcgcgg agacctcgtt catcgccgtt
    13921 gccgcctgcg aaagctcttc atcctgacgg gctgcctcgg cctcggtggc cacggcgatc
    13981 tgcgcggtct ggctggccag cgcgctgatc ttctgcgcgg cgtcactcac ccgcaccagc
    14041 accgtgcgaa tgtgggcgtc gcggtactgc tggcccagtt ccagttgtgc cagctcatca
    14101 cccgcgccga acagcacctg cgcggtcaac aggtttggat agcgcttgcg ctcggcttca
    14161 gccaccttgc gcagccgggc agtggtccaa tggatgccaa acccacccac catggccgcc
    14221 gcaccccacc accacagcga catgaagccg ccactccagt ctggaaccat gcctggaacc
    14281 gccgccagcc ccgcggtggt caggatcaag gcagccactc gcgctggcaa tcccagaatc
    14341 ggtgagcgca gtgtgacggt cggcgccttg tcgcgcagcg tggcgtagag ccgttcggcg
    14401 cgctcgatgt aatcacgttg cggcagcacc cgcaccgact gatagcccac cgcctgacca
    14461 tcctgcacaa tcggggtgac ataggccaac acccagtagt ggtcgccgtt cttgcaacgg
    14521 ttcttcacca cccccaacca gggttcaccg cgcttcagct tcgcccacat ctgctgaaaa
    14581 gcggcaggcg gcatgtcggg atggcggatc acattgtgcg gctggccaat cagctcctcg
    14641 cgggtaaaac cgctgagggc gacaaagtca tcgttgacat aggtgatgcg gccacccagg
    14701 tcggtacagg agatcaactg ctggctcggt tcgaacagcc gctcgtgctg gctgacgggt
    14761 tgattgttgc gcataagacc ctctttgttc ttgcttgctt gttgtcgatc agtgaaatca
    14821 gctcttcggt tccagtgccc tgccgattca gcagaaaatt ttctgtcatt aaaaacatag
    14881 cagaagagtc aggtatgagt gggtccgtgg gaaaaagagg ggggaattcg aaccgtgaaa
    14941 cccgagggcg cttgaaaaaa accggcaacg gccccattct gtcagcacac gttagggggg
    15001 cgacttggct tcgacgccgg ttgcaaggcc caaggtgcat gccgagaggg tagtgactct
    15061 cgtaaatcaa tcgctgcaac tactatagtt gccaacgacg aaacctacgc cctggccgct
    15121 taagccggcc agccttcgac cagactgtgc ttgtgcagat ctggaattcg gaggtcacac
    15181 aatcacaaga tcgcagcggc gctcgcctgg ggcaaggctg tcaaaaaact ccaggaccat
    15241 cgaagacaac cctgtcggtc gggtcgtctt cggtcagatc aatagaccag actaagcatg
    15301 tagatctgag ggttgaggac tgacggacgc gggttcgatt cccgccgcct ccaccaataa
    15361 tgaaacccca accgttctcg gttggggttt tttcttgcct gatcgcgccg gttcccgcgt
    15421 gttgttgggg ggttcctgca gactgcgcca gccgcccgaa ttggccgttc cgggccacat
    15481 tccactcctc ctgaccattc ctcgctccga cctcgcttcc tgaaaccggc ccgaagtccg
    15541 caagggtcgc actttaaact tatataaatc aattagttga gtcatgacgg atcaagcggt
    15601 tcactgacca cctctgacgc aaagcgccaa actggtcacc attcaacgcg gcgatgcgct
    15661 ggttgatacc tgcaggcaag attcctcgca tgactcgatg tcgacctgcc tgtagagcac
    15721 ccgaccgcag agcttcagga acttgggtcc gatcccctcg ctgcgccagc gctcaaggca
    15781 ggcctcgctg agatgccagc gcgcggccag ttgcttttga ttgaggtgaa cgacgtccat
    15841 cttggtctcc ttccgaatga gttggcagga gtccatgaa
//
LOCUS       DAORCQ010000051        15749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2375_length_15749_cov_6.106856, whole genome shotgun sequence.
ACCESSION   DAORCQ010000051 DAORCQ010000000
VERSION     DAORCQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15749
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_15749_cov_6.106856"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="PKI23_06880"
     CDS             complement(<1..101)
                     /locus_tag="PKI23_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNN36697.1"
                     /translation="MAKAQLRITEIFLSLQGEGPSVGWPTTFIRLTG"
     gene            complement(125..847)
                     /gene="ybgF"
                     /locus_tag="PKI23_06885"
     CDS             complement(125..847)
                     /gene="ybgF"
                     /locus_tag="PKI23_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNN36698.1"
                     /translation="MALQPLPLLAAEAPPAAAASSGGGSVSSELFLKLQQMEQEILEL
                     RGRIEEQSHDIEQLKTRQKEHYLDFDRRINELKSPAAAAPAAAGETAPVQPTAAAPQQ
                     TAAAPASTPAAAATGNAEEAYKSAYQLVKERRFDEARSSFEAFVQRFPKGDYTANAYY
                     WLGELYLLKSDWEAGRRSFTKVIGDYPQHPKTPDAMYKLGVVYHKMGQNDRAKEMLQK
                     TRTIFPDSSAARLAEIYLREIK"
     gene            complement(902..1441)
                     /gene="pal"
                     /locus_tag="PKI23_06890"
     CDS             complement(902..1441)
                     /gene="pal"
                     /locus_tag="PKI23_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNN36699.1"
                     /translation="MSLSRLLTLALLMVSLVGLAGCSSKGDIDETAAGGAAAGMGGQG
                     GASSYGAGAGGGIGAHGGSGGIPSSPAELVKVIYFDFDQDAIRDEYREQVVAHALYLK
                     NNPQASLTIEGHCDERGTREYNLALGERRAKAVRNFLLLEGVASNQIEVVSYGEERAE
                     NPEHSEEAWSQNRRAQFRY"
     gene            complement(1492..2820)
                     /gene="tolB"
                     /locus_tag="PKI23_06895"
     CDS             complement(1492..2820)
                     /gene="tolB"
                     /locus_tag="PKI23_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNN36700.1"
                     /translation="MTCLLSSAPAALRRRWRPFGLLALLLSLLGWSRLGVANLTIEIT
                     SGVDNPIKVAIVPFRGAEGVDDVAGIVSGDLERTGQFSAIARNNMLSTPHEEQEVVMR
                     DWRLLGADYVAIGQLQRTANGQSEVRYLLFNTATGARLLAETLTGRSDQTRALAHRVS
                     DRIYEAITGIPGIFSTQMIYITESRSAGAMRYRLFLADADGERPQVLLDSREPILSPA
                     WAPDGERIAYVSFETGRPAIYVQNVRSGQREQVTNFSGLNSAPDWSPDGRRLALVLSK
                     DGDPEIYILDLATRNLKRVTSHFAIDTEPRWLPDGESLIFTSNRGGKPQIYQLDLDSL
                     AVKRVTYEGDYNARGTVTADGRTLVFVHRSGGNFHIAAQDLKRGNVRILSDTRLDESP
                     SVSPNGTMVLYATETRGQGILAAVSIDGRVRFRIPATEGDVREPAWSPLR"
     gene            complement(2826..3824)
                     /gene="tolA"
                     /locus_tag="PKI23_06900"
     CDS             complement(2826..3824)
                     /gene="tolA"
                     /locus_tag="PKI23_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNN36701.1"
                     /translation="MASTSRSQEWSLPVAGAVLLHVAIALLLALNWRSEPRVVEQPRA
                     IQATLITPQPTRSAATGGRSAARPTPPEPVRPEPPKPKPEPPKPQPPQPQPKPEPPKP
                     EPPKPEPKPEPPKPQPKAELPKPPPKPEPAKPQPKPEPPKAEPAKPQSKPEPAKPQPK
                     PEPPKPQPKQESPKPVAKPDAAPKPADERQKALADLANALRSEEAKNAPATAPAPAAA
                     PGKVDQPAAGSSADAKVDDKEIAKYKALITQEIQQNWSRPPGTRPDMKVELEIRLGPG
                     GELLTARVVQSSGSEAFDRSALQAVNRINTFPVPEEPRLFEHFRTLNLVFKPEDLP"
     gene            complement(3826..4248)
                     /gene="tolR"
                     /locus_tag="PKI23_06905"
     CDS             complement(3826..4248)
                     /gene="tolR"
                     /locus_tag="PKI23_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNN36702.1"
                     /translation="MAQLRGKRGPMAEMNVVPYIDVMLVLMVIFMVTAPMLTQGVVVN
                     LPSASTEPVPEQKEPIVVSVDATGSYYIDSAADPKQAVDLAAVQAFVGKVVQQRPDVA
                     IYVRGDQQVDYGKVVTLMAALQAVGAKSVGLITQPAEK"
     gene            complement(4251..4964)
                     /gene="tolQ"
                     /locus_tag="PKI23_06910"
     CDS             complement(4251..4964)
                     /gene="tolQ"
                     /locus_tag="PKI23_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNN36703.1"
                     /translation="MPASSELSIISLIIHATLPVQAVMLTLFLASFFSWVFIIQRGRA
                     LGRARKEMAAFEEQFWSGIDLGKLYRDGNARANDGQLFTGLELLFRAGFKEFFRLRQQ
                     EGADPEAVLDGVQRAMRVSLSKEQEKLEAQLPFLATVGSTSPYVGLLGTVWGIMNSFR
                     GLSTVQQATLATVAPGIAEALVATAIGLFAAIPAVVAYNRYSAMAEGLMANYQTFTEE
                     FSNILHRQLHSRGPNRLGE"
     gene            complement(4976..5422)
                     /gene="ybgC"
                     /locus_tag="PKI23_06915"
     CDS             complement(4976..5422)
                     /gene="ybgC"
                     /locus_tag="PKI23_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNN36704.1"
                     /translation="MSSQPEHPAAVPFSLAIRVYIEDTDAGGRVYYVNYLKYMERARS
                     EWLRRLGFEQQTLRAQNLLFVVHSLELRYHHPARLDQLLQVTADLIETGRASLLFAQQ
                     VLDAASGGCLASGQVRIAAVAADSGRPLPLPEPLRHALVSPPSLSP"
     gene            complement(5419..6459)
                     /gene="ruvB"
                     /locus_tag="PKI23_06920"
     CDS             complement(5419..6459)
                     /gene="ruvB"
                     /locus_tag="PKI23_06920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNN36705.1"
                     /translation="MIESERIVSAQPKGREEGSDLSIRPARLGDYIGQAAVREQLEIF
                     IGAARARGEALDHTLIFGPPGLGKTTLANIIANEMGVAVKSTSGPVLERAGDLAALLT
                     NLNRGDVLFIDEIHRLSPLVEEVLYPALEDFQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGLLTSPLRDRFGIVQRLEFYDTPALALIVTRAAAILGIAIDPQGATEIACRARG
                     TPRIANRLLRRVRDFAEVKGDGHITRELADRALNMLSVDQLGCDPIDRRLLLTLIDKF
                     GGGPAGIDSLAAAIGEERGTLEDVVEPFLIQQGFIIRTPRGRVVTQQAYLHFGLQPPA
                     VTGDAGQGALPL"
     gene            complement(6488..7108)
                     /gene="ruvA"
                     /locus_tag="PKI23_06925"
     CDS             complement(6488..7108)
                     /gene="ruvA"
                     /locus_tag="PKI23_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNN36706.1"
                     /translation="MIGRISGILLERHPAGLLVDVQGLGYEVKVPLSTLFALPIVGER
                     VTLHTHLASREDGQTLYGFLTLADRELFRELIKVTGVGPKLALTILSGVSAEQFVRAV
                     RAQDGSALVRLPGIGKRTAERLLLELRDRLDDWQVAGAGTGPSDAGTRSPVVEVSPMQ
                     EAEQALVALGYKPQEAARAVSRAQQEGEPSTSEALIRRALRSMVGG"
     gene            complement(7105..7680)
                     /gene="ruvC"
                     /locus_tag="PKI23_06930"
     CDS             complement(7105..7680)
                     /gene="ruvC"
                     /locus_tag="PKI23_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNN36707.1"
                     /translation="MKVLLGIDPGSRVTGYGVIQAEAGRVDYLDSGCIRMSTVTMPVR
                     LGEIFTGVTTLIDRYRPVELAIEQVFLARNADSALKLGQARGAAIAAAVSRGLPVAEY
                     SARQIKQAVVGKGAAEKQQVQHMVRELLRLERAPASDAADALAAAICHAHTLQSGALL
                     LLQSTDRSRRARSRRGTALLAVHPARPESDR"
     gene            complement(7723..8469)
                     /locus_tag="PKI23_06935"
     CDS             complement(7723..8469)
                     /locus_tag="PKI23_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNN36708.1"
                     /translation="MAGHSKWANIKHRKAAQDARRGKVFTRLIREITIAARLGGPLPA
                     DNPRLRSAVDKALGSNMTRDTIDRAIQRGAGAGDEQQLDEVTYEGYGPGGVAILVEAM
                     TDNRNRTVAEVRHAFAKHGGNLGTDGSVAYLFSKSGQITFAPGADEEKILEIALEHGA
                     TDVISEEDGSIEVLTPPHDLLTVKDALVAAGCRPDSSEVVMLPATQVEVEGEAGEKVA
                     RLLERLEDLDDVQNVFTNANLSEALLAGLE"
     gene            complement(8497..10275)
                     /gene="aspS"
                     /locus_tag="PKI23_06940"
     CDS             complement(8497..10275)
                     /gene="aspS"
                     /locus_tag="PKI23_06940"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNN36709.1"
                     /translation="MMRSHYCGDLSSSQVGEQVTLCGWVHRRRDHGGVIFLDLRDRAG
                     IVQLVFDPDNQVAFANADRVRNEYVVRVAGRVRPRPAGTENSEMATGAIEVNGVELEI
                     LNSADTPPFQLDGHTEAGEEVRLRHRYLDLRRPELAQRLRFRGQVTSLIRRQLEEAGF
                     IDIETPILTRETPEGARDYLVPSRTHPGSFFALPQSPQLFKQLLMVAGFDRYYQVARC
                     FRDEDLRADRQPEFTQIDLEASFVDETQIMTITESMVRQLFRRLLEVELPPLPRMTHA
                     EAMRRYGSDRPDLRNPLELVDVADLMQGVEFKVFSAPANDPQGRVAALRLPGGGSLSR
                     KEIDDYTQFVAIYGAKGLAYIKVNALAQGLAGLQSPILKFIPEAVVMRLIERVGAVDG
                     DLIFFGADQARVVNDALGALRLRLGADRGLLQGDWAPLWVVDFPLFERNDDGSWSSPH
                     HPFTQPACSTEEMMRDPAAALSRAYDLVLNGIELGGGSIRIHDARMQQAVFDLLGIGR
                     EEAETKFGFLLAGLRHGAPPHGGLALGLDRMVMLMTGAQSIREVIAFPKTQTAACLMT
                     QAPSVVGEPQLRELNIRLRTVDSNRK"
     gene            complement(10321..10611)
                     /locus_tag="PKI23_06945"
     CDS             complement(10321..10611)
                     /locus_tag="PKI23_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNN36710.1"
                     /translation="MPIYEYRCERCQHKLEAIQKISDEPLVDCPACGQPGLKKLISAA
                     GFRLKGGGWYETDFKSGSKKGLADSAGSGSESTSGASSTSETTSSSSASSAE"
     gene            10936..11289
                     /locus_tag="PKI23_06950"
     CDS             10936..11289
                     /locus_tag="PKI23_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNN36711.1"
                     /translation="MTTKKAPATKSAPIAEKYTKTQILADIAENTSLTRKQVSDVLDE
                     LAVVIERHLGKKGAGEFVLPGLLKIKAVKKPAKKARKNVPNPFKPGEFMDVAAKPAST
                     SVKVLPLKRLKDMAD"
     gene            complement(11705..12892)
                     /locus_tag="PKI23_06955"
     CDS             complement(11705..12892)
                     /locus_tag="PKI23_06955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNN36712.1"
                     /translation="MREVVIVDSVRTGLAKSFRGSFNMTRPDVMLAHCIDALLDRNPA
                     LDPALVDDNIVGAASHDGYQGMNIGRTSVILSKYPITVSGLSLNRYCSSGLQSIAIAA
                     NQIASGFADVITAGGVEQVTLTQGKSNSEGLLNEQIAKTAPGIYMPMGQTAEVVAKRY
                     KVSRESQDEVAYQSQIRTARAQKEGLFKDEIVPMKTTYMEVDKATGETKHIETVVDRD
                     ECNRPSTTLEGLAALKPVFDPEHGTVTAGNSSQLSDGASMTLVMAADMAEKLGLQPLA
                     YFRGFTVAGCNPDEMGIGPVFSVPKLLQRAKLKIDQIDLIELNEAFASQVVYCRDQLG
                     ITMDQLNVNGGSISIGHPYGMTGSRLTGHLCRELKRQKKRYGMVTMCIGGGMGASGLF
                     EAC"
     gene            complement(13173..13670)
                     /locus_tag="PKI23_06960"
     CDS             complement(13173..13670)
                     /locus_tag="PKI23_06960"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNN36713.1"
                     /translation="MVNQSAFDFSVRSIEGEPVALDRYRGKVLLLVNVASRCGLTPQY
                     ADLEALYTQYRDRGLEVLGFPCNQFMNQEPGSEAEIQQFCQTQYGVQFPLFAKIEVNG
                     SGRHPLYQWLTAAAQSQPGDIQWNFEKFLIDRTGQVAARFAPRVSPLDATLIEALERT
                     LATTQ"
     gene            13745..15196
                     /locus_tag="PKI23_06965"
     CDS             13745..15196
                     /locus_tag="PKI23_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007949840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNN36714.1"
                     /translation="MQPAPRLNFDNSFARLPGQFHHRQQPDPLPNPYLVAVDVSAAAL
                     IDLDPATLAATDWAEIFSGQRALPGADPLAMVYAGHQFGGYSPRLGDGRGLLLGEVCN
                     RNGERIDLHLKGAGLTPFSRMGDGRAVLRSCIREYLAGIAMAGLGIPSTRALCVIGSD
                     LPVRRERIEPGATLLRLAGSHIRFGHFEYFYHSGDQASLRQLLDYTLERHFPAALDST
                     SPPLTLLCDVIDRTARLIAEWQAVGFAHGVMNSDNMSIIGETLDYGPYGFQDHYRFDH
                     VCNHSDHHGRYAFDQQPAIGWWNLRALALCFSEWIETEAIERALDQYQWRYLHHFERR
                     MRAKLGLMTEQADDRQLMLDLLDLLHAQHVDYSRCFRALSSEENPQQARDLFAQPDAF
                     DAWHRLYARRLQQEEIPAATRAMLMRQVNPKYTLRNYLAEQAIRQAEQGDFSLIDRLS
                     RLLHQPHDEHPADEAWAGSPPEWGQRLQISCSS"
     gene            complement(15288..>15749)
                     /locus_tag="PKI23_06970"
     CDS             complement(15288..>15749)
                     /locus_tag="PKI23_06970"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNN36715.1"
                     /translation="WKLLGKKELYIPYNNYEMNQPAVKYKDLLTPGHLNQQWSRYELH
                     RVWVVEAKLKADQRHIYSRRVMFLDEDTWQVAEHDMYDGKDQLWRVMEGYHTHFWDPN
                     IGWLQVYSSNDLNAKRYYARGFVNEEKPYEFTTRFSFEDFTPAALRRKGVR"
BASE COUNT         2760 a         5241 c         4953 g         2795 t
ORIGIN      
        1 cagccggtca ggcggatgaa ggtggtgggc cagccgacac tgggcccctc gccctgcaac
       61 gagagaaaaa tctcggtgat acgcagttgc gccttggcca tctggagtgc ggagggctcc
      121 gcggctactt gatttcgcgc aggtagatct cggccagcct ggccgccgaa ctgtcgggga
      181 agatggtgcg ggtcttctgc aacatctcct tggcgcggtc gttctgcccc atcttgtggt
      241 agaccacgcc gagcttgtac atcgcatcgg gggtcttggg atgttgcgga tagtcgccga
      301 tcaccttggt gaacgagcgt cggcccgcct cccagtccga tttcagcagg tagagctcac
      361 ccagccagta gtaggcattg gcggtgtagt cacccttggg aaagcgctgc acaaaggcct
      421 cgaagctgct gcgcgcctca tcgaagcgcc gctccttgac cagttgatag gcgctcttgt
      481 aggcctcttc ggcattgccg gtcgccgccg cagccggcgt gcttgccggt gccgctgcgg
      541 tctgctgggg cgcggcggcc gtcggttgaa ccggagctgt ctcacctgcc gcggccggag
      601 cagccgcagc cggcgacttc agttcattga tgcgtcgatc gaagtcgagg tagtgctctt
      661 tctgccgcgt cttcaactgc tcgatgtcgt ggctctgctc ctcgatgcgg ccacgcagtt
      721 cgaggatctc ctgctccatc tgctgaagct tgagaaacag ctccgaactg acgctgccgc
      781 cgccactgct ggcagcagcc gccggcgggg cttccgccgc aaggagcgga aggggctgca
      841 gcgccatcac gacagccagc agccccaaag caccgaactt cgacctcact gcctcacccg
      901 atcagtagcg gaactgggcg cggcggttct gcgaccaggc ttcctcggag tgctccgggt
      961 tctcggcacg ctcttcgcca tagctgacca cttcgatctg gttcgaggcc accccttcga
     1021 gcagcaggaa gttgcgcacc gccttggccc gccgctcacc gagcgccagg ttgtattcgc
     1081 gggtgccgcg ctcgtcacag tggccctcga tggtcaggct ggcctgcggg ttgttcttca
     1141 gatagagcgc gtgggccacc acctgctcgc ggtattcgtc acggatcgca tcctggtcga
     1201 agtcgaagta gatcaccttg accagttcgg ccggcgagga ggggatgccg ccactgccgc
     1261 cgtgagcgcc gatgccaccc ccggcgccgg ccccatagct ggaggcgccg ccctgaccac
     1321 ccatgccagc cgccgcacca ccggccgcgg tttcatcgat gtcgcccttg gaactgcaac
     1381 cagccaggcc caccaggctg accatcagca gcgcgagagt aagcagacga gacagtgaca
     1441 tgaaattctc ccttggagtg ttgcgaagtg gttgagatcg gtacgaccgc atcagcgcag
     1501 tggcgaccag gccggttcgc gcacgtcacc ttcggtggcg ggaatgcgaa accgcacccg
     1561 gccatcgatc gacacggcgg caagtatacc ctgcccgcgg gtttcagtcg catagagcac
     1621 catggtgcca ttgggcgaaa cactgggtga ctcatcgagc cgggtgtcgg agaggatgcg
     1681 cacattgccg cgcttgaggt cctgcgcggc aatgtgaaag ttgccgccgc tgcggtggac
     1741 gaacaccagc gtgcgcccat cggcagtcac ggtgccacgg gcgttgtagt caccttcata
     1801 ggtgacccgc ttcactgcca gcgaatcgag gtcgagctgg tagatctgcg gcttgccgcc
     1861 gcggttggaa gtgaagatca gcgattcgcc atcgggcagc cagcgcggct cggtgtcgat
     1921 ggcgaagtgg ctggtgaccc gcttcaggtt gcgggtcgcc agatcgagaa tgtagatctc
     1981 cggatcgcca tccttcgaca gcaccagggc cagccggcga ccatcgggtg accagtccgg
     2041 cgcgctgttg aggccggaga aattggtcac ctgctcgcgc tggccgctgc gcacgttctg
     2101 cacatagatg gcgggtcggc cggtctcgaa agagacgtag gcgattcgct cgccatcggg
     2161 tgcccaggcc ggcgacagaa tcggctcccg cgaatcgagc agcacctgtg gccgctcgcc
     2221 atcggcatcg gccaggaaca gccggtagcg catcgcaccg gcggagcgcg actcggtgat
     2281 gtagatcatc tgggtggaga agatgccggg aatgccggtg atcgcctcgt agatgcggtc
     2341 gctgacccga tgcgccagcg cgcgggtctg gtcgctgcgt ccggtcagcg tctcggccag
     2401 caggcgggcg ccggtggcgg tgttgaagag cagatagcgc acctccgact gcccattggc
     2461 agtgcgctgc aactggccga tggcgacata gtcggcgccg agcaggcgcc agtcgcgcat
     2521 caccacctcc tgctcctcat gcggtgtgct cagcatgttg ttgcgggcaa tcgcactgaa
     2581 ctgtccggtt cgctccaagt cgccggagac gatgccggcc acgtcatcca ccccttctgc
     2641 accgcgaaac ggcacgatcg ccaccttgat cggattgtcg acacccgagg tgatctcgat
     2701 ggtcaggttg gcgacaccca gccgtgacca gccgaggagg ctcaacagca gcgccagcag
     2761 tccgaatggc cgccaccggc ggcgcaacgc ggcgggcgcg gacgaaagaa ggcaggtcat
     2821 gcgactcaag ggagatcctc tggcttgaag acgaggttga gggtgcgaaa gtgttcgaac
     2881 agccgcggct cttcgggcac cggaaaggta ttgattctgt tgaccgcctg caaggccgat
     2941 cgatcgaatg cctcactgcc gctcgattgg accacgcggg cagtcagcag ttcaccaccg
     3001 gggccgaggc gaatctccag ctcgaccttc atgtcgggcc gggtgcccgg cggacggctc
     3061 cagttctgct gaatctcttg cgtgatcaat gccttgtact tggctatctc cttgtcgtcg
     3121 accttggcat cggcactgct gccggccgct ggctgatcca ccttgcccgg tgccgccgct
     3181 ggcgccggcg ccgtggccgg cgcattcttc gcctcttcgc tgcgcagcgc attggccaga
     3241 tcggccagcg ccttctgccg ttcgtcggcc ggtttgggtg ccgcgtccgg tttggcgacc
     3301 ggcttgggag actcctgctt cggctgcggc ttgggcggct ccggtttggg ttgaggtttg
     3361 gctggttcgg gcttggattg tggcttcgcc ggctcggcct tgggcggttc gggtttcggt
     3421 tgcggtttcg ctggctccgg tttgggcggc ggcttcggca gctccgcctt cggctgcggc
     3481 ttcggcggct cgggcttggg ctcgggtttg gggggttccg gtttcggcgg ctcgggtttg
     3541 ggttgcggct gcggtggctg cggcttgggg ggttccggtt tcggcttcgg cggctcgggt
     3601 ctgaccggct ccggcggcgt tgggcgggcc gccgaacggc caccggtcgc cgccgatcgg
     3661 gtcggctgcg gcgtgatcag cgtggcctga atcgcacgcg gctgctcgac cacccgcggt
     3721 tcactgcgcc agttcagcgc cagcagcagc gcgatggcga cgtgcaacag cacggcgccc
     3781 gcgaccggga ggctccactc ctgcgatctg gaggtcgacg ccatgctatt tttcggcagg
     3841 ctgggtgatc agccccacac tcttggcgcc caccgcctgc aacgcggcca tcagcgtgac
     3901 caccttgccg taatcgacct gctgatctcc gcgcacatag atggcgacat cggggcgctg
     3961 ctgcaccacc ttgccaacga acgcctgcac ggccgcaagg tcgacggcct gtttgggatc
     4021 ggcagcggag tcgatgtagt agctgccggt cgcatcaacc gagacgacga tcggctcctt
     4081 ctgctcgggc accggttcgg tgctggcgct cggcaggttg accaccactc cctgggtcag
     4141 catcggcgcc gtcaccatga agatcaccat cagcaccagc atcacgtcga tgtaggggac
     4201 gacattcatc tccgccatcg gaccacgttt gccacgaagt tgcgccatga ctactctccc
     4261 agacggttgg gtccacggct gtggagctgg cggtgcagga tgttggagaa ctcctcggtg
     4321 aaggtctggt agttggccat cagcccctcg gccatcgccg agtagcggtt gtaggccacc
     4381 acggccggga tcgcggcaaa caggccgatc gcggtggcca ccagcgcctc ggcgatgccg
     4441 ggggccacgg tggcgagggt cgcctgctgg acggtggaaa gaccacggaa ggagttcatg
     4501 atgccccaga ccgtgccgag caggccgaca taggggctgg tggagccgac cgtggccaga
     4561 aacggcagtt gcgcttcgag cttctcctgc tccttcgaca gcgacacccg catcgcccgc
     4621 tgcaccccgt cgagcaccgc ttccggatcg gccccctcct gctgccgcag acggaagaac
     4681 tccttgaagc cggcgcggaa cagcagctcc agaccggtga agagctggcc atcgttggcg
     4741 cgggcattgc cgtcacggta gagcttgcca aggtcgatgc ccgaccagaa ctgctcctcg
     4801 aacgccgcca tctccttgcg cgcccgcccc agcgcccggc cacgctgaat gatgaagacc
     4861 caggagaaga acgaggcgag gaacagcgtc agcatcaccg cctgcaccgg cagggtggca
     4921 tggatgatca ggctgatgat cgagagttca ctggaagcgg gcacgacgaa aatccttacg
     4981 gggagaggga tgggggtgag accagcgcat ggcgcagagg ttcaggaagt ggcagcggcc
     5041 tgccactgtc ggccgccacc gcagcgattc gcacctgacc gctggcgaga cagcctccgg
     5101 aagcggcatc gagcacctgc tgggcaaaca gcagactggc gcggccggtc tcgatcagat
     5161 cggcggtgac ctgcagcagt tggtcgagcc gggccggatg gtgatagcgc agttcgagcg
     5221 agtggacgac gaacagcagg ttctgcgccc gcaatgtctg ctgctcgaaa ccgagccggc
     5281 gcagccattc gctgcgggca cgctccatgt acttgagata attgacataa tagaccctgc
     5341 cgccggcatc ggtgtcctcg atgtagaccc tgatcgccag gctgaagggc actgcggcgg
     5401 gatgctccgg ctggctgctc acaacggcag tgccccttgc ccggcatcac cggtgaccgc
     5461 cggcggttgc agaccgaagt gcagataggc ctgctgggtc accacccgtc cgcgcggggt
     5521 gcggatgatg aagccctgct ggatcagaaa gggttcgacc acatcctcga gggtgccgcg
     5581 ctcctcgccg atcgcggcgg ccaggctgtc gatgccggcc ggaccaccac cgaacttgtc
     5641 gatcagcgtc agcagcagac ggcggtcgat cgggtcacaa cccaactggt cgacactcag
     5701 catgttgagg gcgcgatcgg ccagttcgcg ggtgatgtga ccatcaccct tcacttcggc
     5761 aaaatcgcgc acccgccgca gcagacggtt ggcgatgcgc ggcgtgccgc gggcacggca
     5821 ggcgatctcg gtcgcgccct gcggatcgat ggcgatgccg agaatcgccg ccgcccgggt
     5881 gacgatcagc gccagcgccg gggtgtcata gaactccagc cgctggacga tgccgaagcg
     5941 gtcacgcagc ggcgaggtga gcaatccggc gcgggtggtg gcgccaacca gcgtgaaggg
     6001 cggcagatcg agcttgatcg agcgcgccgc cggcccctcg ccaatcatga tgtcgagctg
     6061 aaaatcttcg agcgccggat agagcacctc ctcgaccagc gggctcagcc gatggatctc
     6121 gtcgatgaag agcacatcac cgcggttgag gttggtcagc agcgcggcca gatcgccggc
     6181 ccgctccagc accggaccgg aggtggactt gaccgccacc cccatctcgt tggcgatgat
     6241 gttggccagg gtggtcttgc ccagtccggg cggaccgaag atcagcgtgt ggtcgagcgc
     6301 ctcgccgcgg gcgcgggcgg cgccgatgaa gatctcgagc tgctcgcgca ccgctgcctg
     6361 accgatgtag tcaccgaggc gggccgggcg gatcgacagg tcgctgccct cctcgcgccc
     6421 cttgggctgc gccgacacga tgcgctccga ttcgatcatc gacctgctcc gttgacctgc
     6481 cccatgctca gcctcccacc atcgagcgca gtgcccgacg gatcaacgct tcgctggtcg
     6541 agggctcacc ctcctgctgg gcacggctga ccgcgcgcgc cgcctcctgc ggcttgtagc
     6601 ccagcgccac cagcgcctgc tccgcctcct gcatcggcga cacctccacc accggcgacc
     6661 gagtcccggc gtcgctcggg ccagtgccag cgccggccac ctgccaatca tcgaggcggt
     6721 cacgcagctc cagcagcagc cgttcggcgg tccgcttgcc gatgccgggc agccgcacca
     6781 gcgcactgcc atcctgcgcg cgcaccgcgc gcacgaactg ctcggccgag acgccggaga
     6841 gaatcgtcag ggccagcttg gggcccacgc cggtcacctt gatcagttcg cggaacagct
     6901 cgcggtcggc cagcgtcaga aaaccataga gcgtctgtcc atcctcccgg ctcgccagat
     6961 gggtgtgcaa tgtgacccgt tcaccgacga tcggcagcgc aaacagtgta ctgagcggca
     7021 ccttcacctc ataacccaga ccctgcacgt cgaccagcaa cccggccgga tgacgctcga
     7081 gcaggatgcc gctgattcgg ccaatcatcg atcgctctcc ggccgtgccg gatgcactgc
     7141 cagcagcgcc gtgccgcgcc gggaccgggc gcggcgcgac cggtcagtcg actgcagcaa
     7201 cagcagcgcg ccgctctgca gagtgtgggc atggcagatg gccgccgcca aggcatcggc
     7261 cgcatcgctg gccggcgccc gttcgagccg caacagctcc ctgaccatgt gctgcacctg
     7321 ctgtttctcc gccgcgccct tgcccaccac cgcctgcttg atctgtcggg ccgaatactc
     7381 cgccaccggc agaccacggc tgaccgctgc cgcgatcgcg gcgccgcgcg cctgtcccag
     7441 cttcagcgcc gaatcggcgt tgcgcgccag gaacacctgc tcgatggcca gttcaactgg
     7501 ccggtagcgg tcgatcaggg tggtgacgcc ggtaaagatc tcacccagtc gcaccggcat
     7561 cgtcaccgtg ctcatgcgga tgcagccgct gtcgaggtag tcgacacgcc cggcctcggc
     7621 ctgaatcacc ccataaccgg tcacccgcga acccggatcg atgccgagca gcaccttcat
     7681 cggacagata cgccacggat ggaaggcgcg caggtcggca cgtcactcca gcccggccaa
     7741 cagcgcctcg gagaggttgg cattggtgaa gacattctgc acatcatcaa gatcctccag
     7801 ccgctccagc aggcgcgcca ccttctcgcc cgcctcaccc tcgacctcga cctgggtcgc
     7861 cggcagcatc accacttcgc tgctgtcggg ccggcagcca gccgccacca gcgcatcctt
     7921 gaccgtcagc aggtcatgtg gcggcgtcag cacttcgatc gagccatcct cctcgctgat
     7981 gacgtcggtg gcgccatgct cgagtgcgat ctccagaatc ttctcctcgt cggcgcccgg
     8041 cgcgaaggtg atctggccac tcttgctgaa cagataggcc accgagccat cggtgccgag
     8101 gttgccgcca tgcttggcga atgcatggcg cacctcggcc acggtgcggt tgcggttgtc
     8161 ggtcatcgcc tcgaccagga tcgccacccc gccggggcca tagccctcat aggtgacctc
     8221 gtcgagctgc tgctcatcac cggcaccggc accacgctgg atggcgcggt cgatggtgtc
     8281 gcgggtcatg ttgctgccga gcgccttgtc gaccgcgctg cgcaaccgcg gattgtccgc
     8341 cggcagcggg ccacccagac gggccgcgat ggtgatctcg cgaatcagcc gggtgaagac
     8401 cttgccgcgc ctggcatcct gtgccgcctt gcggtgcttg atgttggccc atttgctgtg
     8461 ccctgccatg tcaatcgcgc ctcgcttgaa gctgtgtcac ttgcggttgg agtcgaccgt
     8521 gcgcagacgg atgttgagct cgcgcaactg cggctcgccg accacgctgg gcgcctgcgt
     8581 catcagacag gcggcggtct gggtcttggg gaaggcgatc acttcgcgga tcgactgggc
     8641 gccggtcatc agcatcacca tccggtcaag gccgagcgcc agtccgccat gtggcggcgc
     8701 accatggcgc aggcccgcca acaggaagcc gaacttggtt tcggcctcct cgcggccgat
     8761 gccgagcagg tcgaacaccg cctgctgcat cctggcgtca tgaatgcgga tcgaaccgcc
     8821 gcccagctcg atgccgttca gcaccaggtc ataggcgcgt gacagcgcgg ccgccggatc
     8881 acgcatcatc tcctcggtcg agcaggccgg ctgggtgaag gggtggtggg gcgagctcca
     8941 gctgccatcg tcattgcgct cgaacagcgg aaagtcgacc acccacagcg gtgcccagtc
     9001 gccctgcagc agcccccggt cggcaccgag ccgcagccgc agtgcgccga gtgcgtcatt
     9061 gaccaccctg gcctggtcgg caccaaagaa gatcaggtcg ccatcgaccg ccccgacccg
     9121 ctcgatcagc cgcatcacca ccgcttcggg aatgaacttg agaatcggcg actgcaagcc
     9181 cgccagcccc tgcgccagcg cgttcacctt gatgtaggcc agccccttgg cgccatagat
     9241 ggcgacgaac tgggtgtagt cgtcgatctc cttgcggctc agtgaccccc cgcccggcag
     9301 gcgcagcgcc gcaacccgcc cttgcggatc attggccggc gcgctgaaga ccttgaactc
     9361 gaccccctgc atcagatcgg ccacatcgac cagctccagc gggttgcgca ggtcgggccg
     9421 gtcgctgccg taacgccgca tcgcctcggc atgggtcatg cgtggcaacg gtggcagttc
     9481 gacctccagc agccgccgga acagttggcg caccatcgac tcggtgatcg tcatgatctg
     9541 tgtctcgtcg acaaaggagg cctccaggtc gatctgggtg aactccggct ggcgatcggc
     9601 ccgcagatcc tcgtcacgga aacatctggc cacctgatag tagcggtcga aaccggccac
     9661 catcagcaac tgcttgaaca gctgcggtga ctgcggcagc gcgaagaaac tgcccggatg
     9721 ggtgcggctc ggcaccagat agtcgcgcgc cccttccggc gtctcacggg tcaggatcgg
     9781 tgtctcgatg tcgatgaaac cggcctcttc cagctggcgg cggatcagac tggtcacctg
     9841 gccacgaaag cgcagccgct gcgccagttc gggccggcgc agatcgagat agcgatgacg
     9901 caaccgcacc tcttcacccg cctcggtgtg gccatcgagc tggaacggcg gcgtgtcggc
     9961 gctgttgaga atctccagct cgacaccgtt cacctcgatg gcaccggtcg ccatttcgct
    10021 gttctcggtc cccgctggcc ggggccgtac tcgaccggca acgcgcacca catactcgtt
    10081 gcgcacccga tccgcgttgg cgaaggccac ctgattgtcc ggatcgaaca ccagttgcac
    10141 gatgcccgca cggtcgcgca gatcgagaaa gatgaccccg ccatggtcac ggcgacggtg
    10201 gacccagcca cagagggtga cctgctcgcc cacctggctg ctgctcaaat cgccacagta
    10261 atggctacgc atgatgatga ttgttcctga gacgaaaacg gaaggcgatg aagggcgacg
    10321 ctattcggcc gaagaggcac tggacgacga ggtggtttca ctggtgctgc tggcgccaga
    10381 ggtcgactca ctgccactgc cggcactgtc ggccaaccct ttcttgctgc cgcttttgaa
    10441 gtcggtttca taccagccgc cccccttgag gcgaaagccg gcggcggaga tcaatttctt
    10501 cagtccgggc tggccgcagg ccgggcaatc gaccagcggc tcgtcactga tcttctgaat
    10561 ggcttcgagc ttgtgctggc agcgttcgca acggtactca taaataggca tggagtcgct
    10621 cctgattctc gacagatatc gaaccgggcc caacagaccg ggccactccg caaggggtgg
    10681 gaattatacc cgagtcttgc atcgagacat ccgcatcatg gtcccgccag cgatgaagat
    10741 cgcagtgacg ccgccaacgt gatgaaaaac gcttttttga ccgcgtgccg cttgcacgca
    10801 ccgcaatcga taggctataa cgcactgcgt tttgggcggt tcggtcaggg cctgccacca
    10861 ctggctgctt cgccccggcc cccaccaatt gcctgactcc acttatttca tccccatatg
    10921 cgccaggatt ctctcatgac aacaaaaaaa gcccccgcca ccaagagcgc tccgattgcc
    10981 gaaaagtaca ccaagaccca gattctggcc gacatcgccg aaaacacctc actgacccgc
    11041 aaacaggtca gcgatgtgct cgacgaactg gcggtggtga tcgagcgtca cctgggcaaa
    11101 aaaggagccg gcgaattcgt gctgcccggc ctgctgaaga tcaaggcggt caagaagccg
    11161 gccaagaagg cgcgcaagaa tgtgcccaac ccattcaaac cgggggaatt catggatgtg
    11221 gccgccaagc cggcctcgac cagcgtcaag gtgctgccgc tcaagcggct caaggacatg
    11281 gcagactgat ggccggcagc gctctctgat caaggcgggc gacccggacc agccgttcgg
    11341 tctcgcccgc gccttattcg agtgatgttt ctgatcgaac ctgccaggac tcgggctgga
    11401 gcaccggcct gaaacagccc attccgacct ctgcacccgc agattttctg cgtcctgcgc
    11461 tgcgctgatg cgcattcgca ggcaacggcc ctgttcaaaa accgacacac caggcaggtc
    11521 ggacagcccg gaagcatcca ggcgttgaat gaaaaatccg gcgaacaggc gcggtgccgg
    11581 ttcgacaccg cgtcatgcag agggaagaag agaaaacgcc gtcaaactct tgcgctgtcc
    11641 cagcagcagc ccggttcccc ggaccactgc tgttttcaca gcatcccact gtcgcagagg
    11701 cgactcagca ggcttcgaac agaccgctgg cgcccatgcc gccaccgatg cacatcgtca
    11761 ccatgccata acgcttcttc tgccgcttca actcacggca cagatggccg gtcagacgcg
    11821 aaccggtcat gccgtagggg tgaccgatgc tgatcgaacc gccgttgaca ttgagctgat
    11881 ccatcgtgat gccgagttga tcgcggcagt agaccacctg cgaggcaaag gcttcgttca
    11941 gttcgatcag gtcgatctga tcgatcttca gcttggcacg ctgcagcagc ttgggcaccg
    12001 agaagaccgg gccgatgccc atctcgtcgg ggttgcagcc cgcaacggtg aagccacgga
    12061 agtaggccaa gggttgcagc cccagctttt cggccatgtc ggccgccatc accagcgtca
    12121 tcgaggcgcc atcggagagc tgcgaggagt tgccggcggt cacggtgcca tgctccggat
    12181 cgaagaccgg cttcagcgca gccagccctt ccagcgtggt cgaggggcgg ttgcactcat
    12241 cgcggtccac cacggtctcg atgtgcttgg tctcgcccgt cgccttgtcg acctccatgt
    12301 aggtggtctt catcggcacg atctcatcct tgaacagccc ctctttctgg gcgcgggccg
    12361 tgcgaatctg gctctgatag gccacttcgt cctgtgactc gcggctcacc ttgtagcgct
    12421 tggcgaccac ttcggcggtc tgccccatcg gcatgtagat gcccggcgcg gtcttggcga
    12481 tctgctcgtt cagcagccct tcgctgttgc tcttgccctg ggtcagggtg acctgctcga
    12541 caccaccggc ggtgatcaca tcggcaaagc cgctggcgat ctggttggcg gcgatggcaa
    12601 tgctctgcaa gccggatgag cagtaccggt tcaggctcag accagagacc gtgatcgggt
    12661 atttcgacag gatgaccgag gtgcggccga tgttcatgcc ctgatagcca tcgtggctgg
    12721 cagcccccac gatgttgtcg tcgaccagcg cggggtcgag cgccggattg cggtcgagca
    12781 gcgcatcgat gcaatgcgcg agcatcacat caggccgcgt catgttgaag ctgccacgaa
    12841 acgatttggc aaggccggtg cggacgctgt ccacgattac gacttcacgc atggtgttct
    12901 ccctgtattg ttgtgatttt gtctgtcaac ccgcgccagg caagcaggac ggcggggttg
    12961 gccgcagcgg accgcgcaag aacggggtgt ggctgaagcg ggctgacggc gcacaagagc
    13021 agtcgggttg ttatcggtct ggttcgggtc ccgcactgct gccagcatgg tacgtaagcc
    13081 ctccgggttg ttcaagagaa cgccgaggtc acctgctgac aatgaaaact tccttgatct
    13141 gcgacaaccg cagccgtcgg agcgaatcgg cctcactgcg tcgtcgcaag cgtccgctcg
    13201 agggcttcga tcagggttgc atccagcggc gacacccgcg gcgcgaagcg cgcagccacc
    13261 tgcccggtgc gatcgatcag aaatttctcg aaattccatt gaatgtcacc cggctgcgac
    13321 tgggcggcag cggtcagcca ttgatagagc ggatgccggc cgctgccatt gacctcgatc
    13381 ttggcaaaga gcggaaactg caccccgtac tgcgtttggc agaactgctg aatctccgct
    13441 tcactgcccg gctcctggtt catgaactga ttgcagggaa aacccagcac ctccagcccg
    13501 cggtcgcggt actgcgtata gagcgcctcg agatcggcgt actgcggtgt caagccacag
    13561 cgactggcca cattgaccag caacagcacc ttgccccgat aacgatcgag cgccaccggt
    13621 tcgccctcga tgctcctcac cgaaaaatcg aatgcacttt ggttgaccat ctgcgatacc
    13681 ctccgacaat atccaagcgt tatactcgga cttcagcccc gattccagca ccgggagagc
    13741 cggaatgcaa cccgccccac gactcaactt cgacaacagc tttgcccgcc tgccgggaca
    13801 gttccaccac cggcaacaac ccgatccgct gcccaacccc tatctggtcg cggtcgatgt
    13861 ctcggctgcc gcgctgatcg atctcgaccc tgccacgctg gcggccacgg actgggcgga
    13921 gattttcagc ggtcagcgcg cactgccggg cgccgatccg ctggcgatgg tctatgccgg
    13981 ccatcagttc ggcggctaca gcccgcggct gggcgatggg cgcggactgc tgctcggcga
    14041 ggtctgcaat cgcaatggcg agcgcatcga cctgcatctg aagggcgccg gtctgacgcc
    14101 cttttcgcgc atgggcgatg gccgcgcggt gttgcgctcc tgcatccgtg aatatctggc
    14161 cggcatcgcg atggccggtc tgggcatccc cagcacgcga gcactctgcg tcatcggcag
    14221 tgacctgccg gtgcggcgcg agcggatcga accaggcgcc acgctgttgc ggctggccgg
    14281 cagccacatc cgctttggcc atttcgagta cttctaccac agcggcgacc aggcctcgtt
    14341 gcggcaactg ctcgactaca ccctcgaacg ccactttcct gccgcgctcg acagcacctc
    14401 gcccccgctg acactgctgt gcgacgtgat cgatcgcacc gcgcggctga ttgccgagtg
    14461 gcaggcggtc ggcttcgctc atggcgtgat gaacagcgac aacatgtcga tcatcggcga
    14521 aaccctcgac tacggcccct acggctttca ggatcactac cgcttcgacc atgtctgcaa
    14581 ccactccgac caccatgggc gctacgcctt cgatcagcag ccggccattg gctggtggaa
    14641 cctgcgcgcg ctggcgctct gtttcagtga atggatcgag accgaagcga tcgaacgggc
    14701 gctcgaccag taccagtggc gctacctgca ccacttcgag cgcagaatgc gggccaagct
    14761 cggattgatg accgaacagg cggatgaccg ccaactcatg ctcgatctgc tcgacctgct
    14821 gcatgcccag catgtcgact acagccgctg cttccgggca ttgagcagcg aggagaaccc
    14881 acaacaggca cgtgatctct tcgcgcagcc cgatgccttc gatgcctggc accgcctcta
    14941 cgcacgacgg ctgcaacagg aggagatccc agccgccacg cgcgccatgc tcatgcgaca
    15001 ggtcaacccc aaatacacgc tgcgcaacta cctggccgaa caggcgatcc gccaggcgga
    15061 acagggcgac ttcagcctga tcgatcgact gtcccggctg ctgcaccaac cgcatgacga
    15121 acatccggcc gatgaagcct gggccggttc gccacccgaa tggggccaac gactgcaaat
    15181 cagttgctca tcctgatctg ctgtaccccg gaatccagcg cgcacaaaaa aggccggcct
    15241 cctgcgagac cggcctggtc gggtctgtca gccaggcctg ccgaaactca gcgcacccct
    15301 ttgcgccgca gtgccgccgg tgtgaagtcc tcgaagctga acctggtggt gaactcgtag
    15361 ggcttctctt cgttcacgaa accacgcgca tagtagcgct tggcattcag gtcgttggag
    15421 ctgtagacct gcaaccagcc gatgttgggg tcccagaagt gggtgtggta accctccatc
    15481 acccgccaca gctggtcctt gccgtcatac atgtcatgct cggccacctg ccaggtgtcc
    15541 tcgtccagga acatcacccg ccggctgtag atgtggcgct ggtcggcctt cagcttcgcc
    15601 tccaccaccc acacccggtg cagctcgtaa cggctccact gctggttgag gtggcccggt
    15661 gtcagcagat ccttgtactt caccgccggc tggttcatct cgtagttgtt gtaggggatg
    15721 tagagctcct tcttgcccag cagcttcca
//
LOCUS       DAORCQ010000052        15650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2395_length_15650_cov_6.159731, whole genome shotgun sequence.
ACCESSION   DAORCQ010000052 DAORCQ010000000
VERSION     DAORCQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15650
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2395_length_15650_cov_6.159731"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKI23_06975"
     CDS             complement(<1..646)
                     /locus_tag="PKI23_06975"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNN36716.1"
                     /translation="MKAQGIEIDLPRLRPSRGQLLDHFASQSGAPVAATLNPDDVTAG
                     LISRENKRESAILMPIVQKADELEIILTWRSAHLRSHAGQVSFPGGRVDESDASREHT
                     ALRETEEEIGIPMNRVQVLGRMGDYYTQSGYSITPVVGLIDTPVELRPNPHEVAAIIE
                     APLHFFLDSAAYRIHQRETDGTVRSFYSVTYGDYFIWGATAAMLVEFYRRLAAFM"
     gene            complement(627..2018)
                     /gene="pabB"
                     /locus_tag="PKI23_06980"
     CDS             complement(627..2018)
                     /gene="pabB"
                     /locus_tag="PKI23_06980"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNN36717.1"
                     /translation="MSAPERLAVDYRIDRARLFASLRHWPAAVWLDSNAERRGDGAHD
                     LISACPTVTLQQRQERCWLQPHDGAAAAAAGEFLSLLREQLAKRSDPDGHAAGAAIGY
                     LGYDLARSWAHGAFSTDLARADIDLPQARIGLYDWLYVADHRRQQAEVLIHPACPPAI
                     AERVRGWVAGLAGDTAPPPARFRLEGGWQSDLPQERYRTAFRRVHDYIEAGDCYQVNL
                     AQRLSARYVGDPFEAYLRLRAVSPAPYAAFIGLDEGAILSLSPEQFLQVTAGEVTTRP
                     IKGTRPRLDDRQADEAMRQSLLHSDKERAENVMIVDLLRNDLGKSCQPGSIRVPKLCA
                     LESFANVHHLVSTVVGRLTADRDALDLLRGCFPGGSITGAPKQRAMQIIEELEPHRRA
                     IYCGSIAAIGYDGSLRSSITIRTLLCQRGTVHVWGGGGIVADSSCEAEYQESFAKIDN
                     LCQELNRHESPGN"
     gene            2319..3206
                     /gene="prpB"
                     /locus_tag="PKI23_06985"
     CDS             2319..3206
                     /gene="prpB"
                     /locus_tag="PKI23_06985"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNN36718.1"
                     /translation="MPQPPTAGARFRAALVNHHPLPVVGTINAYSATMAQKIGHQAIY
                     LSGAGVANASYGLPDLGITSLNDVLTDVSRITAACDLPLLVDIDTGWGSAFNISRTIK
                     EMIRAGAAAVHIEDQVAAKRCGHRPNKAIVSKGEMVDRIKAAVDGRTDPDFVIMARTD
                     ALAVEGMEAAVERALLCVEAGADAIFPEAMRELEQYRTFVEAVKVPVLANITEFGATP
                     LFSCEQLGAVGVAMVLYPLSAFRAMNRAAERVYRTILAEGSQQKVVETMQTRDELYAY
                     LDYHAYEQKLDELFKSESE"
     gene            3206..4336
                     /gene="prpC"
                     /locus_tag="PKI23_06990"
     CDS             3206..4336
                     /gene="prpC"
                     /locus_tag="PKI23_06990"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HNN36719.1"
                     /translation="MATAKPLSGAGLRGQVAGQTALCTVGKEGAGLTYRGYPIELLAE
                     KARFEEVAYLLLRGKLPNQAELDGYVAKLKGLRALPQPLKTVLENIPRSAHPMDVMRT
                     GCSMLGNLETELDFAEAHDRIDRMLALFPSIICYWYRFSHDGIRIDTVTDDDSIAGHF
                     LHLLHGKKPSATHQRVLDVSLILYAEHEFNASTFTARVCASTLSDIHSCVTGAIGSLR
                     GPLHGGANEAAMEMIERFGSADEATREIQRMLVAKEKIMGFGHAIYREFDPRNPIIKA
                     WAKRLADEVGDSRLYPVSEAIEKVMWDEKKLFPNADFYHASAYHFMGIPTKLFTPLFV
                     MSRVSGWCAHVLEQRSNNRIIRPSAEYTGPDSAEWVDIAQRG"
     gene            complement(4401..>4880)
                     /locus_tag="PKI23_06995"
     CDS             complement(4401..>4880)
                     /locus_tag="PKI23_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HNN36720.1"
                     /translation="SISQDLNYKIRIDAVNFAQDNDDGGRTRNYDTADIILVGVSRCG
                     KTPTCLYLALQFGIKAANYPITEEDLGELKLPKALLEYRQKLHGLTIAPERLCAIRNE
                     RLPNSKYASLHQCQHEIQEAEAMYRRHGIPFIDSTHYSIEEISTRILAAANIERRLH"
     assembly_gap    4881..4980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4981..5371)
                     /locus_tag="PKI23_07000"
     CDS             complement(<4981..5371)
                     /locus_tag="PKI23_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HNN36721.1"
                     /translation="MKRTVFFISDGTGITAETMGKSLLSHFETLSFSYKTIPYLTAAM
                     AEEVRLRIDAVHREDGLRPIVIATIIDETLLQSIRCANAFFIDLLSTFLRPLEEELQT
                     PSSHTVGKYHSISQDLNYKIRIDAVNFA"
     gene            5611..7989
                     /gene="ppsA"
                     /locus_tag="PKI23_07005"
     CDS             5611..7989
                     /gene="ppsA"
                     /locus_tag="PKI23_07005"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNN36722.1"
                     /translation="MDKYLVWFKEVGMHDVGQVGGKNASLGEMIRNLSEAGVKVPQGF
                     ATTARAYYDFIHENDLFDRIQSALKALNVDDVNALAATGARIRQWILDAPLPKRLEDE
                     VRQAFRTLTDDKDSFAVAVRSSATAEDLPTASFAGQQETYLNIRGIDQTLLSIKRVYA
                     SLFNDRAIAYREHQGFAHDQVGISAGIQKMVRSDLGCSGVLFTLDTESGFRDVVFVTA
                     SYGLGEPIVQGAVNPDEFYLHKPTLQAGRPAVLRRNLGAKAIKMVYRSTADHHETVAT
                     VDVPAAERQRFCISDTDLATLARTGMLIEQHYGRPMDVEWAKDGEDGEIYIVQARPET
                     VKSNSGNAVVERYLLKEQGRVLLEGRSVGHKISSGTVRVITSLQQMDQVQEGDVLVTD
                     MTDPDWEPVMKRARAIVTNRGGRTCHAAIIARELGIPAVVGCSHATELLKNGQQVTVS
                     CAEGDTGRIYEGALDFSVRANSVSSMPNLPVQIMMNVGNPDRAFDFCGLPNEGVGLAR
                     LEFIINRMIGVHPKALLNYASLSADLKQSIDRRMAGYASPVDFYVDKLVEGIATLAAA
                     FAPKKVIVRLSDFKSNEYAHLIGGTQYEPTEENPMLGFRGASRYISDQFRDCFELECR
                     ALKRVREEMGFTNVEVMVPFVRTVGEARQVVELLASNGLKRGEHGLRIIMMCELPTNA
                     LLAAQFLEHFDGFSIGSNDLTQLTLGLDRDSAIVAHLFDERNDAVKALLQMAITTCKS
                     MGKYIGICGQGPSDHLDFAKWLMDQGIDSMSLNPDTVMDAWITLADHLNNHG"
     gene            7982..8239
                     /gene="tusA"
                     /locus_tag="PKI23_07010"
     CDS             7982..8239
                     /gene="tusA"
                     /locus_tag="PKI23_07010"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HNN36723.1"
                     /translation="MAEPNRPAAPVAVQHQLDATGLYCPEPVMMLHDQVRVMQQGEVV
                     EVIATDPSTTRDIPKFCTFLGHELVEQRQQDELFTYYIRKG"
     gene            8242..9144
                     /locus_tag="PKI23_07015"
     CDS             8242..9144
                     /locus_tag="PKI23_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN36724.1"
                     /translation="MSGGRPPAVAAPSPAEELEFDLGTLRIAAKGWGEHDATPLLALH
                     GWLDNAASFDRLAPLLPHLRLVALDMPGHGLSSHRPAGCRSHIWDDLPELLQCADQLG
                     WQRFHLLGHSRGAGIATLLAAAVPERVISLTLIENFLPGTTEDAAAPAQLTAALHDEA
                     LLPGKKKPIYRSVAEAVEARTRIIGEISLEAAELLTARGLMQVEGGYSWRADPRLRLA
                     SMVKLTPAQARAFAAAVAAPTLLILAQGGRFDRMAELDQQLDAFRRLELHRLAGNHHL
                     HLERQAPEVAALLADFIQRHPGDG"
     gene            9169..9972
                     /locus_tag="PKI23_07020"
     CDS             9169..9972
                     /locus_tag="PKI23_07020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNN36725.1"
                     /translation="MTLGATLLAHEPAVRLTLFIALLLLMALWESVAPRRPQRIARLL
                     RWSNNLALAVLNTLLLRLGFPLLAVPFASLAQQRGWGLFNLIDLPPWLAGIVALLLLD
                     LAIYLQHRLFHAVPLLWRLHRLHHADLEFDVTTGLRFHPGEILLSMAIKLAVVALLGA
                     PAIAVLLFELLLNGTALFNHGNVRLPERLDRWLRRLLVTPDMHRVHHSIDPDETDSNF
                     GFNLSCWDRLFGTWRAQPGLGHERMVIGLERFRAPRELWLDRMLLQPLR"
     gene            10047..10478
                     /locus_tag="PKI23_07025"
     CDS             10047..10478
                     /locus_tag="PKI23_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNN36726.1"
                     /translation="MTTFTPISALIGGLMIGGSAALLLLGSGRIAGISGIAFGLLPAS
                     NGERGWRLMFLGGLLLGTLLAWQLPTLTFAPRQGFPLWALLVAGFLVGTGTRLGNGCT
                     SGHGICGLGRRSPRSLAATLTFVLVAMVTFFLLRHVLGVLS"
     gene            10475..10888
                     /locus_tag="PKI23_07030"
     CDS             10475..10888
                     /locus_tag="PKI23_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNN36727.1"
                     /translation="MKARVAALISGILFGLGLTVSQMVNPKKVLGFLDVAGQWDPSLL
                     LVMGGGVLVTVLSFPWIQRRGRPLWAERFSLPTRSDIDRNLLLGAALFGIGWAIAGYC
                     PGPAIAALLINPAEAVPFVLAMLLGAWLQPRWLGR"
     gene            complement(10910..11500)
                     /locus_tag="PKI23_07035"
     CDS             complement(10910..11500)
                     /locus_tag="PKI23_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNN36728.1"
                     /translation="MVPAKHAATVVPLRDGVDGLEILLLRRHARLSFAGGAWVFPGGH
                     VDPEDWPTDAPDDHHGAARVAAVREAREEAGLELLLHQLITLSHWTTPLDQPKRFATW
                     FFAAPAHHGAQVQVDGVEIDDHRWLRPQTALDTHQRGEIFLPIPTYATVELLLDFANV
                     DTALAALAARPPEIVPVKAQSPRQEFARVFAGRPPP"
     gene            complement(11521..12117)
                     /locus_tag="PKI23_07040"
     CDS             complement(11521..12117)
                     /locus_tag="PKI23_07040"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNN36729.1"
                     /translation="MKQPNGKVATPIPAATVVPLRDGDDGVEILLLRRNARLDFVGGA
                     WVFPGGRLDEEDYAQAPGRDELIAARHAAVREAHEESGLTLALDQLVCLSHWTTPGSS
                     PKRFATWFFLAPLSQPQQVVIDGSEIHEHRWYRPQAALDAYLRDEIFIIMPTYHTIEA
                     LIGFADCAELLAHYRNAAPTIVPPEHGKIMPLPTGRRH"
     gene            complement(12214..14367)
                     /locus_tag="PKI23_07045"
     CDS             complement(12214..14367)
                     /locus_tag="PKI23_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNN36730.1"
                     /translation="MQNAKSLFSYRKHWAARFGVTPFLPMSRDEMEQLGWDSCDVILV
                     TGDAYIDHPSFGMALVGRLLEAQGFRVGIISQPDWHSADAFRALGPPALFFGITSGNM
                     DSMVNRYTSDRRRRSDDAYTPGGAPDRRPDRAVIVYAQRCREACAGVPVVLGGIEASL
                     RRIAQYDYWSDKVRRSVLVDSKADILLFGNAERALVELTHRLARGEPIEQITDLRGTA
                     FLRRHGALPPGWSEIDSSLLDAPGRIEPPVDPYAMTPVGGEPACASGEPATAQVIRFE
                     PGKRRLDRDRTVVRLPDFEVVKEDAVLYAHASRTLHLESNPGNARALVQRHGDRDLWL
                     NPPPIPLTTAEMDGVFDLPYARAPHPAYGDARIPAWEMIRFSVNIMRGCFGGCTFCSI
                     TEHEGRIIQSRSEASILHEIEEIRDKTPGFTGVISDLGGPTANMYRLQCKDPQIESSC
                     RRLSCVHPGICPNLNTDHTPLIQLYRKARTLPGIKKVLVASGLRYDLAVRSPEYVREL
                     VTHHVGGYLKIAPEHTEPGPLSVMMKPGIGSYEAFREMFERFSREAGKEQYLIPYFIA
                     AHPGTTDEDMLNLALWLKQHNFRLDQVQTFLPTPMALATAMYRSGRNPLKSLKRDERL
                     RIPKGERTRRLHKAFLRYHDAENWPLLREALRRMGRGDLIGNGRHHLVPSWQPAGTGQ
                     TPEGARAPRRPASGPSRSPAARGAGRTLPKRKGRG"
     gene            14482..14904
                     /locus_tag="PKI23_07050"
     CDS             14482..14904
                     /locus_tag="PKI23_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36731.1"
                     /translation="MHEPAVVAAQPTADRLGDAGEVVTAFDACPQTKRRVETDLEPKT
                     LKTGHFGGWPPAAQMAGTLRSAGILAKKKGPEIVRALQFGGGVEPRTKRLSKGTPIYT
                     HCMGFADRLAANGIFYRRTRCGEYLAHAEIEGHDHPHE"
     gene            14885..>15650
                     /locus_tag="PKI23_07055"
     CDS             14885..>15650
                     /locus_tag="PKI23_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNN36732.1"
                     /translation="MIIRMNSGGYAYIWQAGDWPNWHFDLGALAGPMAEVSRAQGLLM
                     GRLADVGVALRDQASLAALTEDVVKTSEIEGEQLNVESVRSSIARRLGVDIGALAPVD
                     RHVEGVVEMVLDATANCHAPLSRERLFGWHAALFPAGYSGLSRIKVGGWRDDASGPMQ
                     VVSGPIGRQRVHFEAPPAGRLETETSRFMSWLNSASSEPPLLKAGLGHLWFVTLHPFD
                     DGNGRIARAISDLLLARADGSPQRFYSLSAQIQRERK"
BASE COUNT         2645 a         4986 c         5151 g         2768 t
ORIGIN      
        1 ccatgaaggc cgccagccgc cggtagaact cgaccagcat cgcggcggtg gcgccccaga
       61 tgaagtagtc gccataggtg accgaataga aggagcgcac cgtgccgtcg gtctcgcgct
      121 ggtggatccg gtaggcggcg gagtcgagaa agaagtgcag cggcgcctcg atgatggcgg
      181 ccacctcatg ggggttcggc cgcagttcga ccggcgtgtc gatcagcccc accaccgggg
      241 tgatggagta gccgctctgg gtgtagtagt cgcccatccg gcccagcacc tgcacccgat
      301 tcatcgggat gccgatctcc tcttcggtct cgcgcagcgc ggtgtgctcg cggctggcgt
      361 cgctctcatc gacccggccg ccgggaaagc tgacctgacc ggcatggctg cgcaggtggg
      421 cgctgcgcca ggtgaggatg atctccagtt catcggcttt ttgcacgatc ggcatcagaa
      481 tggccgattc gcgcttgttt tcgcgggaga tcagcccggc ggtgacatcg tcggggttca
      541 gggtggccgc gaccggcgcg ccactctggc tggcgaagtg gtcgagcaac tgcccgcgac
      601 tcggccgcag gcgtggcagg tcgatctcaa ttccctgggc tttcatgccg gttcaactcc
      661 tggcagaggt tgtcgatctt ggcgaaggac tcctgatact ccgcctcaca actggagtcg
      721 gccactatgc caccgccgcc ccacacatgg acagtcccgc gctgacagag cagggtgcgg
      781 atggtgatgc tgctgcgcag cgagccgtcg tagccgatcg cggcgatcga gccgcagtag
      841 atggcgcgcc ggtgcggctc cagttcttcg atgatctgca tcgcccgctg cttgggggcg
      901 ccggtgatcg aaccgcccgg aaagcagccc cgcaacagat cgagtgcatc gcggtcggcg
      961 gtcagccggc ccaccaccgt gctgaccaga tggtggacat tggcgaagct ctccagcgcg
     1021 cagagtttgg gaacccggat gctgcccggc tggcagctct tgccgaggtc gttgcgcagc
     1081 aggtcgacga tcatcacatt ctcggcccgc tccttgtcgc tgtgcagcag gctctggcgc
     1141 atcgcctcgt ccgcctgccg gtcatccagc cgtggccggg tgcccttgat cggccgggtg
     1201 gtcacctccc cggcggtgac ctgcagaaac tgctcgggcg acagactgag aatggccccc
     1261 tcatcgagcc cgatgaaggc ggcatagggg gcggggctga cggcgcgcag ccgcaggtag
     1321 gcctcgaagg ggtcgccgac atagcgcgcg ctgagccgct gcgccagatt gacctggtag
     1381 cagtcgcccg cctcgatgta gtcatgcacg cgccggaagg cggtgcgata gcgctcttgc
     1441 ggcaggtcgc tctgccagcc accctccagc ctgaatcgcg ccggcggcgg ggccgtgtcg
     1501 ccggcgagcc cggccaccca gccgcgcacc cgctcggcaa tcgccggcgg gcaggccgga
     1561 tggatcagca cttcggcctg ctggcgtcga tgatcggcca cgtagagcca gtcgtagagg
     1621 ccgatgcggg cctgcggcag gtcgatgtcg gcccgcgcca gatcggtcga gaaggcgcca
     1681 tgcgcccagc tgcgggccag gtcgtagccg aggtagccaa tcgcggcgcc ggctgcgtgg
     1741 ccgtctggat cactccgttt ggccaactgc tcgcgcaaca gactgaggaa ttctccggcc
     1801 gccgctgccg ctgcgccatc atgcggttgc agccagcagc gctcctgccg ttgctgcagc
     1861 gtcacggtcg gacaggccga gatcagatca tgcgcgccat cgccgcgccg ctcggcattg
     1921 ctgtcgagcc agacggcagc gggccagtgg cggagggagg caaacagccg ggcgcggtcg
     1981 atccggtagt cgactgccag gcgctcgggt gcactcattc cgggatcggc tgcgggacag
     2041 agcgtgggtc acgccagggc gacagccctg cacggattgt ggtcgctgag accctcgatg
     2101 cgttgacagc actggagcga atcattagag tccatgcccc agttcagcgg ccgggctgcg
     2161 tggttcccct tgccccggcg cggcgggaca gccggtaaaa aaccggccag atccacggtt
     2221 cagcatgacc atctcgttct ctccagactt ggcggcagcc gccaggccgg ctccggtgag
     2281 cccggcgtga tatgtgccga tattaaagga atggaatgat gccacaaccc ccgaccgcag
     2341 gcgcccgttt tcgcgccgca cttgtcaacc accatccgct gccggttgtc ggcaccatca
     2401 acgcctacag cgccacgatg gcccagaaga ttggccatca ggcgatctac ctctctggcg
     2461 ccggcgtcgc caatgcctcc tatggcctgc ccgatctggg catcacctca ctcaatgatg
     2521 tgctgaccga tgtcagccgt atcaccgctg cctgcgatct gccgctgctg gtcgacattg
     2581 acaccggttg gggcagcgcc ttcaacatct cgcgcacgat caaagagatg atccgcgccg
     2641 gcgcggcggc ggtccatatc gaggaccagg ttgccgctaa gcgctgtggc caccgcccca
     2701 acaaggccat cgtcagcaag ggcgagatgg tcgatcggat caaggctgcg gtcgatggcc
     2761 gcaccgatcc agacttcgtg atcatggcgc gcaccgacgc gctggcggtc gaggggatgg
     2821 aggccgcggt cgaacgtgcg ctgctctgcg tcgaagcggg cgccgatgcg atcttccccg
     2881 aggcgatgcg cgaactggag cagtaccgca ccttcgtcga ggcggtcaag gtgccggtgc
     2941 tggccaacat caccgagttc ggcgcgaccc cgctgttcag ttgcgagcag ttgggcgccg
     3001 tcggtgtggc catggtgctc tatccgctga gcgcattccg cgccatgaac cgggcggccg
     3061 aacgggtgta ccgcaccatc ctggccgagg gcagccaaca gaaggtggtc gagacgatgc
     3121 agacccgcga tgagctgtat gcctatctcg attaccatgc ctatgaacag aaactggacg
     3181 aactattcaa gtcggagtcg gaatgatggc aacagcgaaa ccactctcgg gtgcaggact
     3241 gcggggacag gttgcggggc agacggcgct ctgcaccgtg ggcaaggagg gcgccggcct
     3301 cacctatcgg ggctatccga tcgagcttct ggccgagaag gcgcgtttcg aggaggtggc
     3361 ctacctgctg ctccggggca agctgcccaa ccaggcggag ctcgatggct atgtcgccaa
     3421 gctgaagggg ctgcgcgcgc tgccgcagcc gctgaagacg gtgctggaga acatcccccg
     3481 ctcagcccat ccgatggatg tgatgcgcac cggctgctcg atgctgggca acctcgaaac
     3541 cgagctcgac ttcgccgagg cacatgaccg catcgatcgg atgctggcgc tgtttccgtc
     3601 gatcatctgc tactggtacc gcttcagtca cgatggcatc cgcatcgaca ccgtgaccga
     3661 tgacgattcg atcgccggcc actttctcca tctgctgcat ggcaaaaaac cgagtgccac
     3721 ccatcagcgg gtgctcgatg tttcactgat tctctatgcc gagcatgagt tcaacgcctc
     3781 gaccttcacc gcccgggtct gcgcctcgac gctctcggac atccactcct gcgtgaccgg
     3841 tgccattggc tcgctgcgtg gaccgctgca tggcggcgcc aacgaggcgg caatggagat
     3901 gatcgagcgg ttcggctccg ccgacgaggc cacccgtgag attcagcgga tgctggtggc
     3961 caaggagaag atcatgggct ttggccacgc catctaccgt gaattcgacc cgcgcaatcc
     4021 gatcatcaag gcctgggcga agcggctggc cgacgaagtg ggcgacagcc ggctctatcc
     4081 ggtgtcggag gcgatcgaaa aggtgatgtg ggacgagaag aaactgttcc ccaatgccga
     4141 cttctaccat gcctcggcct accacttcat gggcattccg accaagctgt tcacgccgct
     4201 gttcgtgatg agccgggtca gcggctggtg cgcccatgtg ctggaacagc gcagcaacaa
     4261 ccgcatcatc cgccccagcg ccgaatacac cggccccgac agcgcggagt gggtcgacat
     4321 cgcccagcgc ggttgagcca agcgtgcacc accagagcgg ccgtcatggc cgctcttgcg
     4381 ttggactgaa acaaaaccgc tcagtgcagc cgccgttcga tgttggcggc agccagaatg
     4441 cgggttgaaa tctcctcgat cgagtagtgg gtcgagtcga tgaaaggaat gccatggcgg
     4501 cggtacatcg cctcggcctc ctggatctca tgctgacact gatggagcga ggcatacttg
     4561 ctgttgggca gccgctcgtt gcgaatcgcg cagagccgct ccggggcaat ggtcaggcca
     4621 tggagcttct gcctgtactc cagcagcgcc ttgggcagct tcaactcgcc gagatcctct
     4681 tcggtgatcg ggtagttggc ggccttgatg ccaaattgca gtgccagata aaggcaggtc
     4741 ggcgtctttc cgcagcgcga gacccccacc aggatgatgt cggcggtgtc gtagttgcgg
     4801 gtgcgcccac catcgtcgtt gtcttgggcg aagttgaccg catcgatgcg aatcttgtag
     4861 ttcagatcct ggctgatgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 gggcgaagtt gaccgcatcg atgcgaatct tgtagttcag atcctggctg atggagtggt
     5041 acttgcctac ggtatgtgac gagggcgtct gcaactcctc ttccagtggc ctgagaaagg
     5101 tcgacagcag gtcgatgaag aacgcattcg cacagcggat cgattgcagc agtgtctcat
     5161 cgatgatggt ggcgatgacg atcggccgca ggccatcctc ccgatgcacc gcgtcgatgc
     5221 gtaacctgac ctcttcggcc atcgctgcgg tcagataggg aatggtcttg taggagaacg
     5281 agagggtttc aaagtgggag agcaggctct tgcccatggt ctcggcagtg atgccggtgc
     5341 cgtcggagat gaagaaaact gtccgtttca tgctttactg cctatgattt catagaggat
     5401 acaaccgcgt caggcaagca ttcatcaact ggccctcatt atgcaagtgc gtggttgatt
     5461 caacctgacg cagcagcgaa tcaccttatc gccatggctg cgccttggct aaaatgggcg
     5521 gcccagccgc cagcaggcgc accaatttca gcaacggatg tcgaacgcag gtccgcgtcg
     5581 gtcccaattc atcaacagga gagcgtagcc ttggataagt atctggtctg gttcaaagag
     5641 gtcggcatgc atgacgtcgg ccaggtgggc ggcaagaatg cctcgctggg cgagatgatc
     5701 cgcaacctct ccgaggcggg agtcaaggtc ccgcagggct ttgccaccac cgcccgggcc
     5761 tactacgact tcattcatga gaacgacctg ttcgaccgca tccagtctgc gctcaaggcg
     5821 ctcaatgtcg atgatgtcaa cgcgctggcc gcgaccggtg cacggatccg gcagtggatt
     5881 ctcgatgcac cgctgccgaa gcggctggaa gatgaagtac ggcaggcatt ccggaccctg
     5941 accgatgaca aggactcctt cgccgtggca gtacgctcct cggccaccgc cgaagatctg
     6001 ccaaccgcct cctttgccgg ccagcaggag acctatctca acatccgcgg catcgaccag
     6061 accctgctgt cgatcaagcg ggtctacgca tcgctcttca acgaccgcgc catcgcctac
     6121 cgtgaacacc agggctttgc gcacgatcag gtcggcatct ccgccgggat ccagaagatg
     6181 gtgcgcagcg acctcggctg cagtggcgtg ctcttcaccc tcgacaccga gtcggggttc
     6241 cgtgacgtgg tcttcgtcac cgcctcctac ggcctgggtg agccgatcgt gcaaggggcg
     6301 gtcaatcccg atgagttcta cctgcacaaa ccgacgctgc aggcgggccg tcctgcggtg
     6361 ctgcggcgca acctcggcgc caaggcgatc aagatggtct accgctcgac cgccgaccac
     6421 cacgagacgg tggcgacggt cgatgtgccg gccgcggagc gccagcgctt ctgcatcagt
     6481 gacaccgatc tggccacgct ggccagaacc ggcatgctga tcgagcagca ctatggccgc
     6541 ccgatggacg tcgaatgggc gaaagatggc gaggacggcg agatctacat cgtccaggcg
     6601 cggcccgaaa cggtcaagag caacagcggc aacgcggtgg tggagcgcta cctgctcaag
     6661 gaacagggcc gggtgctgct cgaagggcgc agcgtcggcc acaagatcag cagcggcacg
     6721 gtgcgggtga tcacctccct gcagcagatg gatcaggtgc aggagggcga tgtgctggtc
     6781 accgacatga ccgatcccga ctgggagccg gtgatgaaac gggcgcgggc catcgtcacc
     6841 aaccgcggtg gccgcacctg tcacgccgcc atcattgccc gcgagctggg cattccggcg
     6901 gtcgtcggct gcagccatgc caccgaactg ctgaaaaatg gccagcaggt gaccgtctcc
     6961 tgcgccgaag gcgataccgg ccgcatctac gaaggggcgc tcgatttttc ggtccgtgcc
     7021 aacagtgtgt cgagcatgcc caacctgccg gtgcagatca tgatgaacgt cggcaacccc
     7081 gaccgcgcct tcgatttctg tggcctgccc aacgaagggg tggggctggc gcggctcgag
     7141 ttcatcatca accggatgat cggcgtccac cccaaggcgc tgctcaacta cgcgagcctg
     7201 tcggccgatc tcaaacagag catcgaccgg cgcatggccg gctatgcttc accggtcgat
     7261 ttctatgtcg acaagctggt cgagggcatc gccacgctgg cggcggcctt tgcgcccaag
     7321 aaggtgatcg tgcggctctc cgacttcaag tcgaacgaat acgcccacct gatcggtggc
     7381 acccagtatg agccgaccga agagaacccg atgctcggtt tccgtggggc ttcgcgctac
     7441 atctccgacc agttccgcga ctgtttcgag ctcgaatgcc gggcgctgaa gcgggtgcgc
     7501 gaggagatgg gcttcaccaa cgtcgaggtg atggtgccct tcgtgcgcac cgtcggcgaa
     7561 gcacggcagg tggtcgaact gctggcaagc aatgggctga aacggggcga gcatggcctg
     7621 cgcatcatca tgatgtgtga actgccgacc aacgcgctgc tggccgcgca gttcctcgag
     7681 cacttcgatg gcttctcgat cggctccaac gacctcaccc agttgacgct ggggctggac
     7741 cgcgactccg cgatcgtggc ccacctgttc gatgagcgca acgacgccgt caaggcgctg
     7801 ttgcaaatgg cgatcaccac ctgcaaatcg atgggcaagt acatcggcat ctgcggacag
     7861 gggccatcgg accacctcga cttcgccaag tggttgatgg atcagggcat cgacagcatg
     7921 tcgctcaatc ccgacaccgt gatggatgcc tggatcacgc tggccgacca cctcaacaac
     7981 catggctgaa ccgaaccgac cggccgcccc ggttgccgtc cagcatcaac tggatgcgac
     8041 cgggctttac tgccccgagc cggtgatgat gctgcatgac caggtgcggg tgatgcagca
     8101 gggcgaggtg gtcgaggtga tcgccaccga cccctcgacg acgcgtgaca tccccaaatt
     8161 ctgtaccttt cttggccacg aactggtcga gcagcgacaa caggatgagc tgttcaccta
     8221 ctacatccgc aagggttgac gatgtccggc ggccggccac ctgccgttgc ggcgcccagt
     8281 ccggccgaag agctcgaatt tgacctcggc acgctgcgca tcgccgccaa gggctggggc
     8341 gagcatgatg caacaccgct gctggcgctg catggctggc tcgacaacgc cgccagcttc
     8401 gaccgactgg caccgttgct gccacatttg cggctggtgg cgctcgacat gccgggccat
     8461 gggctgtcga gccatcggcc ggccggctgc cggtcgcaca tctgggacga tctgccggag
     8521 ctgttgcagt gcgccgacca gcttggctgg caacgctttc atctgctggg ccattcccgc
     8581 ggtgccggca tcgccaccct gctggcggca gcggtgccgg agcgggtcat cagcctgaca
     8641 ctgatcgaga acttcctgcc gggcaccacc gaggatgccg ccgccccggc gcagctgacc
     8701 gccgcgctgc atgacgaggc gctgctgccc ggcaagaaga agccgatcta ccgcagcgtg
     8761 gccgaggcgg tcgaggcccg cacccgcatc atcggcgaaa tatccctcga agcggccgaa
     8821 ctgctgacgg cccgcggcct gatgcaggtc gaggggggtt acagttggcg tgccgaccct
     8881 cgactgcggc tggcctcgat ggtgaagctg acgccagccc aggcgcgcgc cttcgccgca
     8941 gccgtggccg caccgacgct gctgattctg gcacaggggg ggcgcttcga ccgcatggcc
     9001 gagctcgacc agcagctcga tgccttccgt cggctcgaac tgcatcgatt ggccggcaac
     9061 caccatctgc acctcgaacg gcaggcgccc gaggttgccg cgctgctggc cgacttcatc
     9121 cagcgccacc cgggtgatgg ctgagggggc cgctttcggc cggctgccat gaccctcggc
     9181 gcgacgctgt tggcccacga accggcggtt cgtctcaccc tcttcatcgc actgctgttg
     9241 ctgatggcgc tgtgggagag cgttgcccca cggcgtccac agcgcatcgc ccgactgctg
     9301 cgctggagca acaacctggc gctggctgtg ctcaacacgc tgttgctgcg gctcggtttt
     9361 ccgctgctgg cagtgccgtt cgccagcctt gcgcagcagc gcggctgggg cctgttcaac
     9421 ctgatcgatc tgccgccatg gctggccggc atcgtggccc tgctgctgct cgatctggcc
     9481 atctacctgc aacaccggct gttccatgcc gtaccgctgc tgtggcggct gcaccggctg
     9541 caccatgccg acctcgagtt cgatgtcacc accgggttgc gctttcatcc cggtgaaatc
     9601 ctgctgtcca tggcgatcaa actggcggtg gtcgctctgc tgggtgcgcc ggccattgcc
     9661 gtgctgctgt tcgagctgct gctgaacggc actgccctgt tcaaccatgg caatgtccgc
     9721 ctgcccgagc ggttggatcg gtggctgcgc cggctgctgg tgacgccgga catgcatcgg
     9781 gtccaccact ccatcgatcc ggacgagacc gacagcaact tcggtttcaa tctgagctgc
     9841 tgggatcggc tgttcggcac ttggcgcgcc cagcctggac tgggtcatga gcggatggtg
     9901 atcggcctcg agcggtttcg cgccccgcgc gagttgtggc tcgaccggat gctgctccaa
     9961 ccgctgcgct gaatcgggcc gctgcaccga acctcccggc cggtgtatgc ttccagactc
    10021 gattcacctg cgagcgagca cacaccatga ccaccttcac gcccatttcc gcactgatcg
    10081 gcggtttgat gatcggtggc agtgcggcac tgctgctgct tggcagcggc cgcatcgccg
    10141 gcatcagcgg catcgccttc ggactgctgc cggccagcaa cggcgagcgt ggctggcggc
    10201 tgatgtttct gggcggcctg ctgctcggca ccctgttggc ctggcagctt cccacgctca
    10261 ccttcgcacc gcgtcagggc tttccactct gggcgctgct ggtcgccgga ttcctggtcg
    10321 gcaccggcac ccgcctcggc aatggctgca ccagcgggca tggcatctgc ggccttggcc
    10381 gccgttcgcc gcgctcgctg gccgccaccc tgaccttcgt gctggtggcg atggtcacct
    10441 tcttcctgtt gcgccatgtg ctgggagtgc tgtcatgaaa gcgcgggtgg ctgccctgat
    10501 cagtggcatt ctgttcggcc tggggttgac cgtctcccag atggtcaacc cgaaaaaggt
    10561 gttgggtttt ctcgatgtcg ccggccagtg ggatccgagc ctgctgctgg tgatgggcgg
    10621 tggggtgctg gtcaccgtgc tgagctttcc gtggattcag cgccggggtc gaccgctgtg
    10681 ggccgaacgg ttcagcctgc cgacccgcag tgacatcgac cgcaacctgc tgctgggcgc
    10741 tgccctgttc ggcatcggtt gggcgattgc cggctactgt cccggtccgg cgattgccgc
    10801 cctgctgatc aacccggccg aggcggttcc cttcgtgctg gcaatgctgc ttggcgcctg
    10861 gctgcaaccg cgctggctcg gccgctgaag cacggctgga gcggccggct cagggcggcg
    10921 gtcgtccggc aaagacgcgg gcgaactcct gccgcggact ctgtgccttg accggcacga
    10981 tctccggcgg ccgtgcggcc agtgctgcga gcgctgtgtc gacgttggca aagtcgagca
    11041 gcagttcgac ggtggcatag gtgggaatcg gcagaaagat ctcgccccgc tgatgggtgt
    11101 cgagtgccgt ctgtggccgc agccagcggt ggtcgtcgat ctcgaccccg tcgacctgca
    11161 cttgggcacc gtggtgggcc ggtgccgcga agaaccaggt ggcaaagcgc ttcggctggt
    11221 cgagcggcgt ggtccagtgg ctcagggtga tcagctgatg cagcaacagt tcgaggccgg
    11281 cctcctcgcg cgcctcgcgc acggcggcga cacgggcagc accatggtga tcatctggcg
    11341 catcggtggg ccaatcttcc ggatcgacat gaccgccagg aaagacccag gcaccgccgg
    11401 cgaagctgag ccgggcatgg cggcgcagca acagaatctc cagcccgtcg acgccatcgc
    11461 gcagtggcac cacggtggcg gcatgcttgg cggggaccat cgcggggcct cctgtcgggc
    11521 tcaatggcgc ctgccggtcg gcaacggcat gatcttgcca tgctcgggcg gcacgatggt
    11581 gggtgctgcg ttccggtaat gggcgagcag ttcggcgcag tcggcgaagc cgatcagcgc
    11641 ctcgatggtg tggtaggtgg gcatgatgat gaagatctca tcgcgcaggt aggcgtcgag
    11701 cgccgcctgt ggccggtacc agcgatgttc atggatctcg gagccatcga tcaccacctg
    11761 ctgcggctgc gacagcggcg ccagaaagaa ccaggtggcg aagcgcttgg gactcgagcc
    11821 cggcgtggtc cagtgcgaca ggcagaccag ttggtcgagc gcgagcgtga gtccactctc
    11881 ctcgtgggct tcgcgcactg ccgcatggcg ggcggcgatc aactcgtcgc ggccgggcgc
    11941 ctgggcgtag tcctcttcat cgaggcgtcc gccgggaaag acccaggcac ccccgacgaa
    12001 gtcgagccgg gcattgcgcc gcagcagcag aatctccacc ccgtcatcgc catcgcgcag
    12061 cggcaccacg gtggcggctg gaattggcgt tgccaccttg ccgttcggtt gtttcatctt
    12121 tgcaggccgt tcaccagaag ccggccaggt cggcggctcg aaattccagg ttgaatcgcc
    12181 gcatcatacc cgattcaccc gagcgcacgc ggctcacccg cggcctttgc gtttcgggag
    12241 ggttctgccg gcgccacgcg cggcggggct tcggcttggc ccgctggccg gacggcgcgg
    12301 cgcgcgcgcc ccttcggggg tctggccggt gccggccggt tgccagcttg gcaccagatg
    12361 gtgccggcca ttgccgatca ggtcgccgcg gcccatgcgg cgcagcgctt cgcgcagcag
    12421 tggccagttt tcggcatcgt gatagcgcaa aaaagccttg tgcagccggc gggtgcgctc
    12481 gcccttgggg attctgagcc gctcgtcacg cttcaagctc ttgagtgggt tgcggccgct
    12541 gcggtacatg gcggtggcca gcgccatcgg cgtcggcaga aaggtctgta cctggtcgag
    12601 gcggaagttg tgctgtttca gccagagtgc caggttgagc atgtcttcat cggtggtgcc
    12661 ggggtgggcg gcgatgaagt agggaatcag atactgctcc ttgcccgcct cgcgcgagaa
    12721 gcgctcgaac atctcgcgga acgcctcgta actgccgatg cccggcttca tcatcaccga
    12781 cagtgggccc ggctcggtgt gctccggcgc gatcttcaga tagccgccaa catggtgggt
    12841 caccagttcg cgcacatact ccgggctgcg caccgccagg tcataacgca gccccgaggc
    12901 gaccagcacc ttcttgatgc cgggcagcgt gcgtgccttg cggtagagct ggatcagcgg
    12961 ggtatggtcg gtattgagat tggggcagat tcccggatgg acgcaggaga gccggcgaca
    13021 gctcgactcg atctgcggat ccttgcactg caaccggtac atgttggcgg tggggccgcc
    13081 gaggtcggag atcacgccgg tgaagccggg cgtcttgtcg cggatctcct cgatctcgtg
    13141 caggatcgac gcctccgagc ggctctggat gatccgcccc tcatgctcgg tgatcgagca
    13201 gaaggtgcag ccgccgaagc agccgcgcat gatgttgacc gagaagcgga tcatctccca
    13261 ggccgggatg cgggcatcgc cataggccgg atgaggcgcg cgcgcgtagg gcaggtcgaa
    13321 caccccgtcc atctcggcgg tggtgagcgg aatcggcggt ggattgagcc acaggtcgcg
    13381 gtccccgtgg cgctgcacca gcgcccgcgc attgccgggg ttcgactcca gatgcagcgt
    13441 gcgcgaagcg tgggcgtaga gcacggcatc ttccttcacc acctcgaagt ccggcagccg
    13501 caccacggtg cggtcgcggt cgagccgccg cttgcccggc tcgaagcgga tcacctgtgc
    13561 cgtggccggc tcgccactgg cgcaggccgg ttcgccgccg acgggcgtca tcgcataggg
    13621 atcaaccggc ggctcgatgc gccccggtgc atccagcagg ctggagtcga tctcgctcca
    13681 gcccggtggc agggcgccat gccggcgcag aaaggcggtg ccgcgcaggt cggtgatctg
    13741 ctcgatcggc tcgccccgcg ccagccgatg ggtcagctcc accagcgccc gttcggcatt
    13801 gccgaagagc agaatatcgg ccttgctgtc gaccagcacc gagcggcgca ccttgtccga
    13861 ccagtaatcg tactgggcga tgcggcgcag gctggcctcg atgccgccga gcaccaccgg
    13921 cactccggca caggcttcgc ggcagcgctg cgcatagacg atcaccgccc ggtcgggccg
    13981 acggtccggc gctccgcccg gcgtgtaggc atcatcggaa cggcggcgac ggtcggaggt
    14041 gtagcggttg accatcgaat ccatgttgcc ggatgtgatg ccgaagaaca gcgcaggcgg
    14101 ccccagtgcg cggaatgcat cggccgagtg ccagtccggc tggctgatga tgccgacgcg
    14161 aaagccctgc gcttcgagca gccggcccac cagcgccatg ccgaagctgg ggtggtcgat
    14221 gtaggcatcg ccggtgacca gaatgacatc gcaactgtcc cagccgagtt gctccatctc
    14281 gtcacgggac atcggcaaaa agggtgtgac accgaagcgt gcggcccaat gtttacggta
    14341 agagaagaga cttttggcgt tttgcatcgg cccattctag gctgaagttc cagcgccgtc
    14401 tcggctgttc accccggttc agtccagcag gcgtgctctg ctgccgtcag cgcagagcat
    14461 ccgggccggg aagcagcccc ggtgcatgag cctgctgtgg ttgcagcgca acccactgcc
    14521 gaccgactgg gtgatgcagg ggaagtggta acggcgtttg atgcctgccc gcagaccaaa
    14581 cggagagtag agacagactt ggagccaaag acgctgaaaa cagggcattt cggcggatgg
    14641 ccacccgcag cacagatggc cggaaccctg cgtagtgcag ggattttggc caagaaaaag
    14701 ggcccggaga ttgtccgagc ccttcaattt ggtggtgggg tggaaccccg caccaaacga
    14761 ctatccaagg gcacacccat ttacacccat tgcatgggtt tcgcagaccg tcttgcggcg
    14821 aatggcatct tctatcgccg cacaagatgc ggcgaatacc ttgcacatgc ggagattgaa
    14881 ggccatgatc atccgcatga atagcggtgg ttacgcatac atctggcagg ccggcgactg
    14941 gccgaactgg cacttcgacc tgggagctct ggctgggccc atggccgagg tcagtcgtgc
    15001 ccaggggctc ctgatgggcc gtctggccga cgtgggcgtg gcgctgcgcg atcaggcgag
    15061 cctcgcggcg ctgacggagg atgtggtcaa gaccagcgag atcgagggcg agcagctcaa
    15121 tgtcgaatcc gtgcgttcat ccatcgcccg caggctgggc gtggacatcg gcgcgctggc
    15181 cccggtggat cgtcacgtcg aaggcgtggt cgagatggtg ctggatgcca ccgccaactg
    15241 ccatgcgccg ctgtcacggg agcggctgtt cggctggcac gcagcgctgt ttcctgccgg
    15301 ctactcaggc ctttccagga tcaaggtcgg cggctggcgc gacgatgcca gcggtccgat
    15361 gcaggtggtg tccggtccca tcggccgcca gcgcgtgcat ttcgaggcgc cgcccgccgg
    15421 tcgtctggag accgaaacca gccgattcat gagctggctc aatagcgcat caagcgaacc
    15481 gccgttgctc aaggccggtc ttggccacct ctggttcgtc accttgcacc cgttcgacga
    15541 cggcaacgga cgtatcgcga gggccatctc cgacctgctg ctggcgcgcg ccgacggcag
    15601 cccgcaacgc ttctacagtc tgtcggcgca gatccagcgg gagcgcaagg
//
LOCUS       DAORCQ010000053        15387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2450_length_15387_cov_5.707866, whole genome shotgun sequence.
ACCESSION   DAORCQ010000053 DAORCQ010000000
VERSION     DAORCQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2450_length_15387_cov_5.707866"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKI23_07060"
     CDS             complement(<1..576)
                     /locus_tag="PKI23_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HNN36733.1"
                     /translation="MLKRTPLHAHHVAAGGHLVDFGGWEMPLHYGSQIEEHLQVRRAA
                     GMFDVSHMTIVDLHGPDARDFLRHLLANEVDKLKSIGSALYGLMLNEEAGVVDDLIVY
                     RLAEGYRLVVNCATRDKDLAWLATHGRHFSVTCQERRDLAIIAVQGPAARHRLAELLA
                     LPALLDLPPFMAMQHGDRFIARVFTEIERARS"
     gene            complement(635..1867)
                     /locus_tag="PKI23_07065"
     CDS             complement(635..1867)
                     /locus_tag="PKI23_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HNN36734.1"
                     /translation="MNTACDIAIVGGGMVGLTLAALLREQGLKLLLLDADAPPRDDLP
                     LQPDRYQPRVSALSLASQQLLERVGAWPLMRAQRVSPYQRMVVWDGEGSGQIEFDAAA
                     IQQPALGHIVENEVTRHALLATLADQHGVELRWRSPVTALGSTTGGRPLLQLEDGSTV
                     TAQLLVAADGSHSPLRQMAGFRCRQWSYRQRAIVSTVRSTQPHAATAWQCFRDGAPLA
                     LLPLTDPHHCSIVWSLPEPAADSLLASDEASFNQALTRASEARLGALELADRRYSFPL
                     RQSHSVDYVQGALVLIGDAAHSIHPLAGQGVNLGLLDAAVLAEELGKGVARGLPLNDP
                     LLLQRYQRRRQGENLAMMAAMEGLKRLFAADPLPLRLLRNRGLSLVDRSAPLKRWLIG
                     RASGMAGDLPDIARAVSR"
     gene            complement(1864..3120)
                     /gene="ubiH"
                     /locus_tag="PKI23_07070"
                     /gene_synonym="visB"
     CDS             complement(1864..3120)
                     /gene="ubiH"
                     /locus_tag="PKI23_07070"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HNN36735.1"
                     /translation="MKPPACDVAIIGGGMAGLSLALALSHQCGDDPPRITLIERHPFP
                     QQNEPPLQPSFDARATVLSQGSRQIYQTLGLWELLAPWTTAICQIEVSSQGQFGMAHL
                     DCREAGVEAFGHVIENRALGHVLLAATARHPNITLLAPAEVTALQPLAAGMRLTLTDQ
                     SGPRQLEAALAVIADGGRSGLREALGIAIRRRGYRQHALIANLVTGTPHDGRAFERFT
                     PQGPIALLPLSEGRSALIWTLPEAEIETLMQCSEADFIARLQRCFGLRMGLIEHVGER
                     HHYPLERVEALEQIRAHLLLLGNAAHTLHPVAGQSFNLSLRDTATLAAVLQRARQQDQ
                     PLGALSTLQDYLATRQDDQRQTVALSHQLPRLFGQAPLWLRGGRSLGLLAFDLCLPAK
                     QLFSRFTMGLNPPNPLRAGGHGGAGR"
     gene            complement(3117..4448)
                     /gene="pepP"
                     /locus_tag="PKI23_07075"
     CDS             complement(3117..4448)
                     /gene="pepP"
                     /locus_tag="PKI23_07075"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009395625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNN36736.1"
                     /translation="MSLPAKEFSNRRRALMERMAPNSIAILASAPVRIRNRDVDYLYR
                     QESDFHYLSGFVEPESVLVLLPDREQGRFLLFCRERNPERELWDGYRCGPERAPELYG
                     ADDAFPITDIDDILPGLIEGRDHLYYTLGRDAEFDRRLIDWVNQVRGKVRSGAHPPDQ
                     LIALEHLLHDLRLYKSPAELKLMREAGAISARAHVRAMQVCRPGLWEYQLEAELLHEF
                     IRSGSRAPAYSSIVGSGPNSCILHYRENNRQMADGELVLIDAGCEFEYYASDITRTFP
                     VNGTFSGEQRALYEVVLTAQQAAIDATRPGASWNQPHDASVRVITEGLLALGLLSGEL
                     NELIETEACKPYYMHRAGHWLGMDVHDVGEYKVAGAWRMLEPGMTMTVEPGIYVALNQ
                     PGIDPRWRGIGIRIEDDVAVTRNGVELLTPGVPKQIDEIEALMAEARGRQP"
     gene            complement(4475..5050)
                     /locus_tag="PKI23_07080"
     CDS             complement(4475..5050)
                     /locus_tag="PKI23_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNN36737.1"
                     /translation="MSTSPPDHDQFDELLYLYQVAAEAAEIDGFFAGQLCVAGRQPAS
                     ETLRALWRHLDQPAPEAEPLLEQLGSLQARTALAMRAGDFSYQLLLPDDEAPLGDRLA
                     ALVAWCQGFIAGLTSNGRLNDARMGDALRQMVADVIEISHLDCDECEEGEESERDYMA
                     LSEHLRCTAIALHAELTTDADRTATAPSRLH"
     gene            5371..5745
                     /gene="sdhC"
                     /locus_tag="PKI23_07085"
     CDS             5371..5745
                     /gene="sdhC"
                     /locus_tag="PKI23_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNN36738.1"
                     /translation="MNKKRPVNLDLTTIQLPLPAFTSILHRVSGFALFAAIALLLYLL
                     QRSLSSAQGFAEIKVLLSGIGAKFIIWAVLCMLVYHLVAGIKHLLMDIGIGESLEGGR
                     LGAQAVLAITLLLAVILGFWLW"
     gene            5739..6086
                     /gene="sdhD"
                     /locus_tag="PKI23_07090"
     CDS             5739..6086
                     /gene="sdhD"
                     /locus_tag="PKI23_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNN36739.1"
                     /translation="MVTNVTNFGRSGLHDWLVQRLSALVLAAYTLFLLVFLLINPDLD
                     HARWMELFQQRWVRGFTLLALVALIGHAWVGMWTISTDYLKPLLLRFLFQSGCSLVLF
                     VYLVWGVRIVWSI"
     gene            6090..7862
                     /gene="sdhA"
                     /locus_tag="PKI23_07095"
     CDS             6090..7862
                     /gene="sdhA"
                     /locus_tag="PKI23_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNN36740.1"
                     /translation="MTKLQTLSFDGIIVGGGGAGMRAALQLAQSGYKTAVVSKVFPTR
                     SHTVSAQGGITCAIGSDDPNDDWRWHMYDSVKGSDYIGDQDAIEYMCSVGPQAVFELE
                     HMGLPFSRTETGRIYQRPFGGQSKDFGRGGQAARTCAAADRTGHALLHTLYQANLKNN
                     AVFLNEWYAIDLVVNQRGQVVGVVALCIETGEMAYLQAKATVLATGGAGRIYQSTTNA
                     LINTGDGVGMALRAGFPVQDIEMWQFHPTGIADAGVLVTEGCRGEGGYLINKDGERFM
                     ERYAPNAKDLASRDVVARSMILEILAGRGAGPKGDHVLLKLDHLGPEVLHKRLPGIVE
                     LSKTFAHVDPVKEPVPVVPTCHYMMGGIPTNINAQALTQDSAGTDRIVDGLYAVGEVA
                     CVSVHGANRLGGNSLLDLVVFGRAAGLHIEQALRHGIDSQPASDSDLDAALARVNRWN
                     ASKSGESVAELRAELQKTMQNYFGVFRKEESMKQGIQTIAALRERLRDGFLVDKSMVF
                     NTARVEALELDNLFEVAEATAIAAEARKESRGAHAREDYQERDDENWLKHSIYFPRER
                     RVGKRDVNFSPKTVEPFEPKVRQY"
     gene            7893..8603
                     /locus_tag="PKI23_07100"
     CDS             7893..8603
                     /locus_tag="PKI23_07100"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNN36741.1"
                     /translation="MQVSVYRYNPNTDAEPQMQQVEVEIPAGRDLMVLDVLQLIKEQD
                     PTFAYRRSCREGVCGSDGINMNGKNGLACITPLSSLVKGQGGKLVLRPLPGLPVIRDL
                     VVDMAIFYRQYEKVQPYLINDEVTSGIERLQSPEEREKLDGLYECILCACCSTSCPSF
                     WWNPEKFIGPAGLLQAYRFLADSRDRATDERLAALDDPFSVFRCRGIMNCVNVCPKGL
                     NPTRAIGHIRSMLLRRAT"
     gene            8777..11629
                     /locus_tag="PKI23_07105"
     CDS             8777..11629
                     /locus_tag="PKI23_07105"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNN36742.1"
                     /translation="MTEPSMEMLWRSGHISGGNANYVEALYDSYLRDPNSVPEAWRNY
                     FDSLPQVANVVVQDVPHSTIREQFLQIGRQAHRGELALAADRDHQQLQVRVLQLIDAY
                     RHRGHQKARLDPLGLQQRPAVPDLELTYHGLSAADFSTRFQTGAPSLAQGEATLKEIV
                     EALEQTYCQTVGVEYMHIVDSQQKLWLQQRLESVRSHPDYSVEVKHHLLERLVAAEGL
                     EKLLGSRFPGTKRFGLEGGESLIPLVDALIQRAGGYGAKEIVIGMAHRGRLNVLVNNF
                     GKNPAELFEEFEGLHAHGAGSGDVKYHQGFSSNVMTSVGQTHLALLFNPSHLEIVTPV
                     VAGSVRARQDRRNDPLGNQVVPVAIHGDAAFAGQGVVMETLQMSQVRGYRIGGTIHII
                     VNNQVGFTTSRADDARSTEYCTDVAKMVQAPIFHVNGDDPEAVLFVTQLAVDYRETFR
                     RDVVIDMVCYRRRGHNEADEPSGTQPLMYAKIRTLPSTMQLYSDRLVNSGVISRELVD
                     AWIADYRKALEEGRHVAKSLVTEPNLKLFVDWTPYLGHDYWSATADTRFELRQLQQLA
                     AEVCKVPEGVVVQKQVDKIIDDRRKMIAGALPLNWGFAETMAYATLLTEGHPVRLMGQ
                     DSGRGTFSHRHAVIHNQKDGSPHVPLANIPGVTARFTLHDSLLSEEAALAFEYGYATT
                     NPRALVIWEAQFGDFVNGAQVVIDQFIASGETKWGRLCGLVMFLPHGYEGQGPEHSSA
                     RLERFMQLCAEHNIQVCIPTTPAQIFHLLRRQMLRPLRKPLIVMTPKSLLRHKLAISS
                     LEDLAEGEFKLVIPESAPLDASQVRRIVLCSGKVYYDLIEAREAAQREDVAVIRIEQF
                     YPFPHEQLQQAMAPFIQAQQVIWCQEEPMNQGVWYSSQHHMIHVLGLLNPGWKLDYVG
                     REASAAPATGYLSLHVEQQQRLVKEALGLDRFNDLH"
     gene            11644..12858
                     /gene="odhB"
                     /locus_tag="PKI23_07110"
     CDS             11644..12858
                     /gene="odhB"
                     /locus_tag="PKI23_07110"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNN36743.1"
                     /translation="MMLEIKAPSFPESISDGTVATWHKKVGEAVLRDEVLVDIETDKV
                     VMEVVAPADGRLSEILKPEGETVLSTELIGLFEAGSALTGTQPSAEPPAAAAAQSQQP
                     LVNPAARRLVQAHGLTPEQIAATGKGGRLTKGDVLQHLEQGQPPAQPVPAVSTATSPR
                     AVEPAVVAESGARIEKRVPMTRLRASVARRLVEAQQTAAMLTTFNEVNMQPVMDLRTK
                     YRDQFEKRHGVRLGFMSFFVRASVEALKRFPAVNASIDGNDIVYHGYQDIGVAVSTDR
                     GLVVPILRDAAQMSLARIEAQVRDYGLKARDGKLTIEEMQGGTFTLSNGGVFGSLLST
                     PILNPPQTAILGMHKVQERPMAVDGKIEILPMMYLALSYDHRLIDGREAVQFLVAIKD
                     FLEDPARILLDL"
     gene            12905..14338
                     /gene="lpdA"
                     /locus_tag="PKI23_07115"
     CDS             12905..14338
                     /gene="lpdA"
                     /locus_tag="PKI23_07115"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNN36744.1"
                     /translation="MAGKFDVIVIGAGPAGYVAAIKCAQLGLSTACVEQWRRDGQTVL
                     GGTCLNVGCIPSKALLESAHKYREAKEQLADHGVLAKEVSLDLATMMARKEKIVGNLT
                     GGIQALFKGNGVTTLAGRGRLLAGKRVEVTDAAGAVEEYEAEHVIIAAGSEPIPISQA
                     PLDGHHVVDSSAALAFEEVPKRLGVIGAGVIGLELGSLWSALGAEVVLLEAQERFLPM
                     VDQRIAKEAQKLLTRQGLTIQLGARVTHTEVKEGEVLVRYSNAKGDQALRVDRLIVAV
                     GRRAQTANLLAADCGVNLDERGSIFVNDHCRTDVPGVYAIGDVVRGPMLAHKGMEEGI
                     MVAERIAGQLSQVNYDCIPSVIYTQPEVAWVGDTEEVLKARAEPYKVGSFAFAANGRA
                     LAAHQSEGLVRVLAHAESDRVLGVHIIGPQASELIAQAVVAMEFSASAEDLALTVFAH
                     PTLSEAVHEAALSVNGQAIHAVNRARK"
     gene            14396..>15387
                     /gene="sucC"
                     /locus_tag="PKI23_07120"
     CDS             14396..>15387
                     /gene="sucC"
                     /locus_tag="PKI23_07120"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNN36745.1"
                     /translation="MNLHEYQAKQLFAEYGLPVSKGVVADSAAAAVEAASLIGGEQWV
                     VKAQVHAGGRGKAGGVKLVRSKAEIEQFAQQWLGKRLVTYQTDADGQPVNQILIENCT
                     DIDRELYLGAVVDRASQRVVFMASSEGGVEIEKVAERSPEKILQAIIDPLVGAQPYQA
                     REIAFQLGLNDKQVKQFTDLFVRLSRLFHECDLALLEINPLVITQQGDLHCLDAKINI
                     DGNALYRQPKLKALHDPSQEDAREREAAAWELNYVALEGNIGCMVNGAGLAMGTMDII
                     KHHGGNPANFLDVGGGATKERVSAAFKLILSDEKVKAVLINIFGGIVRCDLIAEG"
BASE COUNT         2717 a         4678 c         5211 g         2781 t
ORIGIN      
        1 ggaacgggct cgctcgatct cggtgaagac gcgggcgatg aagcggtcgc cgtgctgcat
       61 ggccatgaag gggggcaggt cgagcagtgc gggcagtgca agcagttcgg ccagtcggtg
      121 ccgggccgcg gggccctgta ccgcaatgat ggcgaggtcg cggcgctcct ggcaggtgac
      181 gctgaaatgg cggccatgtg tggcgagcca ggcgagatcc ttgtcgcggg tggcgcagtt
      241 gaccaccagg cggtagccct cggcaagacg atagacgatc aggtcatcga ccacgccggc
      301 ctcttcgttg agcatcaggc cgtagagtgc gctgccgatg gatttcagct tgtcgacttc
      361 attggcgagc agatggcgaa gaaagtcgcg ggcgtcgggg ccatgcaggt cgacgatggt
      421 catgtgcgag acatcgaaca tgccagcggc ccgtctgacc tgcagatgct cctcgatctg
      481 cgagccatag tgcagcggca tctcccagcc gccgaaatcg accagatggc cgccggcggc
      541 gacatggtgg gcgtgaaggg gggtccgttt cagcattggc cgtccagaac aaagagcgcc
      601 attctacgcc ggttttgccg tggcagcggc atcatcaccg cgacaccgcg cgagcgatgt
      661 caggcagatc gccggccatg ccgctggcgc gcccgatcag ccagcgcttg agtggcgcgc
      721 tgcgatcgac cagcgacagc ccgcggttgc gcagcagccg cagtggcaac ggatcggccg
      781 cgaacagccg cttcagcccc tccattgctg ccatcatcgc cagattctcc ccctgacggc
      841 ggcgctggta acgctgcaac agcagtggat cgttcagcgg caggccgcgc gccactccct
      901 tgcccagctc ctcggccagc accgcggcat cgagcaggcc gaggttgacc ccctgtccgg
      961 ccagtggatg gatgctgtgg gcggcatcgc cgatcagcac cagtgcgccc tgcacatagt
     1021 cgacactgtg cgactgccgc aacgggaagc tgtagcggcg atcggccagt tcgagcgcac
     1081 ccagccgcgc ttcgctggcg cgggtcagcg cctgattgaa gctggcctcg tcgctggcca
     1141 gcaggctgtc ggccgctggt tccggcaacg accagacgat cgagcagtgg tgcggatcgg
     1201 tgagtggcag cagcgccagc ggcgcgccat cgcgaaaaca ttgccaggcg gtggccgcat
     1261 ggggttgagt cgagcgcacc gtggagacga tggcccgttg ccggtaggac cactgccggc
     1321 agcgaaagcc ggccatctgt cgcagcgggg agtggctgcc gtcggccgcc accagcaact
     1381 gggcggtgac ggtcgagcca tcttccagtt gcagcagtgg cctgccgcca gtggtgctgc
     1441 cgagcgcggt gaccgggctg cgccagcgca gctcgacacc gtgctgatcc gccagcgtgg
     1501 ccagcagggc atggcgggtc acctcgtttt cgacgatgtg ccccagcgcc ggctgctgga
     1561 tggcggcggc atcgaactcg atctgcccgc tgccctcgcc atcccagacc accatccgct
     1621 ggtaaggcga gacccgctgc gcccgcatca gtggccaggc accgacccgt tcgagcagct
     1681 gctgcgacgc gaggctgaga gcgctgacac gtggctggta acggtcgggt tgcagcggca
     1741 ggtcgtctcg tggcggcgca tcggcgtcga gcagcagcag cttcagcccc tgctcgcgca
     1801 gcagtgcggc cagcgtcagt cccaccatgc cgccaccgac gatggcgatg tcgcaggcag
     1861 tattcatcgt ccagcacctc cgtgaccgcc tgcccgcagc ggattgggcg ggttcagccc
     1921 catggtgaag cgggagaaca gctgtttggc gggcaggcag aggtcgaagg ccagcagacc
     1981 gaggctgcgg ccgccgcgca gccacagcgg cgcctggccg aacagccgtg gcagctggtg
     2041 gctcagtgcc acggtctgcc gttgatcgtc ctggcgagtt gccagatagt cttgcagggt
     2101 cgagagtgcg cccagcggct ggtcctgctg ccgtgctcgt tgcagcacgg cagcgagggt
     2161 ggcggtgtcg cgcagcgaca ggttgaagct ctgccccgcc accggatgca gggtatgcgc
     2221 ggcattgccc agcagcagca ggtgggcgcg gatctgctcc agcgcctcga cccgctccag
     2281 cgggtagtgg tgacgctcgc cgacgtgctc gatcagcccc atgcgcaggc cgaaacagcg
     2341 ttgcaaccgg gcgatgaagt cagcctcgct gcactgcatg agcgtctcga tctcggcttc
     2401 gggcagcgtc cagatcagcg ccgatcgccc ttcactcagc ggcagcaggg cgatcggacc
     2461 ctgtggcgtg aagcgttcga atgcgcggcc gtcgtgcggt gtgccggtga ccaggttggc
     2521 aatcagcgca tgttgccggt aaccacggcg gcggatggcg atgccgagtg cctcgcgcag
     2581 gcccgagcga ccgccatcgg caataaccgc cagcgccgcc tccagttggc gtggaccgct
     2641 ctggtcggtc agggtcagcc gcatgccggc ggcgagcggt tgcagcgctg tcacctcggc
     2701 cggtgccagc agtgtgatgt tgggatgccg tgcggtggcc gccagcagga catggcccag
     2761 ggcacggttc tcgatcacat ggccgaaggc ctcgacgccg gcttcgcggc agtcgaggtg
     2821 ggccatgccg aactgcccct ggctcgatac ctcgatctgg cagatggcgg tcgtccatgg
     2881 ggccagcagc tcccacaggc cgagcgtctg atagatctgc cggctgccct gtgacagcac
     2941 cgtggcgcgg gcgtcgaagc tgggctgcaa cgggggttcg ttctgctgcg ggaagggatg
     3001 ccgctcgatc agcgtgatgc gcggtgggtc gtcgccgcac tggtggctca gcgccagcgc
     3061 cagactcagt ccggccatgc cgccgccgat gatggcgaca tcgcacgcag gcggtttcat
     3121 ggctgtcggc ctcgggcctc ggccatcagc gcctcgatct catcgatctg tttcggtaca
     3181 cccggtgtca gcagttcgac accgttgcgg gtgaccgcca cgtcatcctc gatacggatg
     3241 ccgatgccgc gccagcgcgg gtcgatgccg ggctggttga gggcgacgta gatgcccggc
     3301 tccacggtca tggtcatacc cggttcgagc atccgccagg cgccggccac cttgtactcg
     3361 ccgacatcat gcacatccat tcccagccag tggccggcgc ggtgcatgta gtagggtttg
     3421 caggcttcgg tctcgatcag ctcgttcagt tcgccgctca gcagtcccag tgccagcagc
     3481 ccctcggtga tcaccctgac gctggcatcg tgcggctgat tccagctggc gccggggcgg
     3541 gtggcgtcga tggcggcctg ctgggcggtc agcaccacct cgtagagggc gcgctgttcg
     3601 ccgctgaatg tgccgttgac cgggaaggtg cgggtgatgt cggaggcgta gtactcgaat
     3661 tcgcagccgg catcgatcag caccagttcg ccgtcggcca tctgccggtt gttttcgcgg
     3721 tagtggagga tgcagctgtt ggggccggaa ccgacgatcg aggagtaggc cggcgcccgg
     3781 ctgccgctgc ggatgaattc gtgcagcagt tcggcctcga gctgatactc ccacaggccg
     3841 ggacggcaga cctgcattgc ccgcacgtgg gcgcgggccg agatggcgcc ggcttcgcgc
     3901 atcagcttca gttcggccgg actcttgtag agccgcaggt cgtgcagcag atgttccagc
     3961 gcaatcagct ggtcgggggg gtgggcgccg ctgcgcacct tgccgcgcac ctggttgacc
     4021 cagtcgatca gccggcgatc gaactcggca tcgcgcccca gtgtgtagta gaggtggtcg
     4081 cgcccttcga tcagtccggg caggatgtcg tcgatgtcgg tgatcggaaa ggcatcgtcg
     4141 gcgccgtaca gctccggcgc ccgttccggt ccgcagcggt agccatccca cagctcccgt
     4201 tccggattgc gctcgcggca gaacagcaga aagcggcctt gctcacggtc gggcagcagc
     4261 accagcaccg actccggctc gacaaagccg ctgaggtagt ggaagtcact ctcttgtcga
     4321 tagagatagt cgacatcacg gtttctgatt cgcaccggcg cgctggcgag gatggcgatg
     4381 ctgttgggcg ccatccgctc catcagggcg cggcggcggt tgctgaactc tttggcgggc
     4441 agactcatgg ggttcccatc gatgaggttc gggttcaatg gagtcgggat ggcgctgttg
     4501 cggtccgatc ggcatcggtt gtgagctcgg catggagcgc gattgccgtg cagcgcagat
     4561 gttcgctcag cgccatgtag tcgcgttcgc tctcctcgcc ctcttcacac tcgtcgcaat
     4621 cgaggtggct gatttcgatc acgtcggcca ccatctgccg cagcgcgtcg cccattctgg
     4681 catcgttcag ccgcccgttc gaggtgagtc cggcgatgaa gccctggcac caggcgacca
     4741 gcgcagccag tcggtcaccg agcggggcct catcatccgg cagcagcaac tggtagctga
     4801 agtcgccagc ccgcatggcc agtgcggtac gggcctggag cgaaccgagc tgctccagca
     4861 gcggctcggc ctcgggcgca ggctgatcga ggtggcgcca cagcgcgcgc agcgtctcgc
     4921 tggcgggctg ccggccggcc acgcagagct gtccggcgaa gaagccatcg atctcggccg
     4981 cctctgccgc cacctgatag aggtagagca gctcgtcgaa ctggtcatgg tcgggtggag
     5041 aggtgctcat ggtgatggag tcgtgatggg tccgaagagg ggcacgcaat cggtgtgccg
     5101 caagggtggc tgtctggctg aagcgcctgg acagcctgtc cgggagatga gcatgttatc
     5161 agtttgcacg gcggatgaag cccgaggatg ggtgttgccg ccggggaggt ccaattgtaa
     5221 tcatggcgct ctggttctat actcccgccc cattccgagc tcctctgccg gcaattctac
     5281 gggccggcga gtccttcaat cggctgtttt tcgatgggcg gaccggcgtt tcgctgtccg
     5341 cgttttgttg tacaacaaag cgagcctgct gtgaacaaaa aaagaccggt caacctcgat
     5401 ctgaccacga tacaactgcc ccttcctgca ttcacctcca tcctgcaccg cgtttccggc
     5461 tttgcgctct ttgccgccat cgcgctgctg ctctatctgt tgcagcgttc gctctcttcg
     5521 gcccaggggt ttgccgagat caaggtgctg ctgagcggca tcggcgccaa attcatcatc
     5581 tgggcggtgc tgtgcatgct ggtctaccac ctggtggccg gcatcaagca cctgctgatg
     5641 gacattggca tcggcgagtc actggagggt ggccggctcg gcgcccaggc ggtgctggcc
     5701 atcacgctgc ttctggcggt cattctgggg ttctggctat ggtaaccaac gtcaccaatt
     5761 tcggacgcag cggtctgcat gactggctgg tgcagcggct ctcggcgctg gttctggcag
     5821 cttacacgct gtttttgctg gtttttctgc tgatcaatcc cgacctcgat catgcgcgct
     5881 ggatggagct gttccagcag cgctgggtgc gcggcttcac gctgctggcg ctggtcgcgc
     5941 tgatcggcca tgcctgggtc ggcatgtgga ccatctccac cgactacctg aagccgttgc
     6001 tgctgcgttt tctgtttcag agtggttgca gcctggtgct gttcgtctat ctggtctggg
     6061 gtgtacggat tgtctggagt atctgatcaa tgacgaagct gcaaactctt tcgttcgacg
     6121 gcatcatcgt cggtggcggt ggcgcgggaa tgcgcgccgc gctgcaattg gcacaatcgg
     6181 gttacaagac ggcggtcgtc agcaaggtgt ttccgacacg ctcgcacacg gtgtcggccc
     6241 agggtggcat cacctgcgcg atcggcagcg atgacccgaa tgatgactgg cgctggcaca
     6301 tgtatgactc ggtcaagggg tcggactaca tcggcgacca ggatgcgatc gagtacatgt
     6361 gcagtgtcgg ccctcaggcg gtgttcgagc tcgagcacat ggggctgccc ttctcgcgca
     6421 ccgagaccgg gcgcatctac cagcggccgt tcggcgggca gtccaaggat ttcggccgcg
     6481 gcggccaggc tgcccgcacc tgcgcggccg ccgaccgcac cggtcatgcg ctgctgcaca
     6541 cgctttatca ggccaacctg aagaacaatg cggtctttct caacgagtgg tacgcgatcg
     6601 atctggtggt caaccagcgc ggccaggtgg tcggtgtggt ggccctgtgc atcgagaccg
     6661 gcgagatggc ctatctgcag gccaaggcca cggtgctggc caccggtggc gccggacgca
     6721 tctaccagtc gaccaccaat gccctgatca acaccggaga tggcgtcggc atggcgctgc
     6781 gcgccggatt cccggtgcag gacatcgaga tgtggcagtt ccaccccacc ggcattgccg
     6841 atgccggggt gctggtgacc gaagggtgcc gtggagaagg cggctatctg atcaacaagg
     6901 atggcgagcg cttcatggag cgctatgcac ccaatgccaa ggatctggcg agccgcgatg
     6961 tggtggcacg ctcgatgatt ctggagattc tggccggacg tggcgccggc cccaagggcg
     7021 accatgtact gctgaagctc gaccacctcg gcccggaagt gctgcacaag cggttgccgg
     7081 gcatcgtcga gctgtcgaag acctttgccc atgtcgaccc ggtcaaggag ccggtcccgg
     7141 tggtgcccac ctgccactac atgatgggtg gcatccccac caacatcaat gcccaggcgc
     7201 tgacccagga cagtgccggc accgaccgga tcgtcgacgg tctctatgcg gtgggtgagg
     7261 tggcctgcgt gtcggtccat ggtgccaacc gtctgggtgg caactcgctg ctcgacctgg
     7321 tggtgtttgg ccgtgctgcc ggactgcaca tcgagcaggc attgcgccac ggcatcgaca
     7381 gccagccggc cagtgacagc gatctcgatg ccgcgctggc gcgggtcaac cgctggaacg
     7441 catcaaaaag cggcgagagc gtggccgagt tgcgtgccga gttgcagaag accatgcaga
     7501 actacttcgg cgtgttccgc aaggaggagt cgatgaagca gggcatccag acgatcgcgg
     7561 cgctgcgcga gcggttgcgc gatggctttc tggtcgacaa gagcatggtg ttcaacaccg
     7621 cacgggtcga ggcgctcgaa ctcgacaacc tgttcgaggt ggcggaggcg acggccatcg
     7681 ccgccgaggc gcgcaaggag agtcggggtg cccatgccag agaggactac caggagcgtg
     7741 atgacgagaa ctggctgaag cactcgatct acttcccgcg cgaacgcagg gtcggcaagc
     7801 gcgacgtcaa cttctctccc aaaactgtcg agcccttcga gccgaaagtc cggcagtact
     7861 aatcgcagtc cgcacccaga cagagtacga cgatgcaagt cagtgtctat cgctacaatc
     7921 ccaacaccga tgccgagccg cagatgcagc aggtcgaggt cgagatccct gcaggccggg
     7981 atctgatggt gctcgacgtg ttgcaactga tcaaggagca ggatcccacc tttgcctacc
     8041 ggcgctcctg ccgagaaggg gtctgcggtt ccgatggcat caacatgaat ggcaagaatg
     8101 gtctggcctg catcaccccg ctctcctccc tcgtcaaggg ccagggcggc aagctggtgc
     8161 tgcggccgct gccgggtctg ccggtgatcc gtgacctggt ggtcgacatg gcgatcttct
     8221 atcgccagta cgagaaggtg cagccctacc tgatcaacga tgaggtgacc agcggcatcg
     8281 agcggttgca gtcgcccgaa gagcgcgaaa agctcgatgg actctatgag tgcatcctct
     8341 gcgcctgctg ctcgaccagt tgcccctcgt tctggtggaa tccagaaaaa ttcatcggtc
     8401 cggccggtct gctgcaggcg tatcgattcc tggccgacag ccgtgaccgt gccaccgatg
     8461 agcggctggc ggcgctcgat gaccccttca gcgtctttcg ctgccgcggc atcatgaact
     8521 gcgtcaatgt ctgtccgaaa gggctgaacc caacccgggc gatcggacac atccgctcca
     8581 tgctgctgag gcgggcgacc tagaggctca agcggtccgt tgcgcgctat catgaaaagg
     8641 ggttgtgttc ggtggaacca atcccttttt tgttgcgcag gcagtcaggg tgatgggctg
     8701 tcgaccatga agcagcaata gcggcatttt ctttgcttta aaaacgcgaa ttgaaattca
     8761 gcaggtgaga tcggcgatga ctgaaccttc aatggagatg ttgtggcgca gtgggcatat
     8821 ctctggcggc aatgccaact atgtcgaggc gctttacgac agctatctgc gcgaccccaa
     8881 cagtgtgccc gaggcgtggc gaaactactt cgactcgctg ccgcaggtgg cgaatgtggt
     8941 ggtgcaggat gtgccccatt cgacgatccg tgagcagttt ctgcaaatcg gacgacaggc
     9001 gcatcgcggc gaattggcgc tggctgccga ccgtgaccat cagcagctcc aggtcagggt
     9061 gctgcaactg atcgatgcct atcgccatcg ggggcatcag aaggcgcggc tcgacccgct
     9121 gggcctgcag cagcggccgg ccgtgcccga tctggagctg acctaccatg gcctgtcggc
     9181 cgcggatttc tccacccggt tccagactgg tgcaccgtcg ctggcgcaag gcgaagccac
     9241 gctgaaggag atcgtcgagg cgctggaaca gacctactgc cagaccgtgg gcgtcgagta
     9301 catgcacatc gtcgacagcc agcagaagct ctggctgcaa cagcggctgg agagcgttcg
     9361 ctcccatccc gactacagcg tcgaggtgaa gcaccacctg ctggagcggc tggtcgcggc
     9421 tgaggggctg gagaagctgc tgggatcgcg ctttcccggc accaagcgct ttgggctgga
     9481 gggtggagag agcctgatcc cgctggtcga tgcactgatt cagcgcgcgg gaggctatgg
     9541 cgccaaggag atcgtgatcg gcatggccca ccgtggtcgc ttgaacgtac tggtcaacaa
     9601 cttcggcaag aatcctgccg aactgttcga ggagttcgag gggctgcatg cccatggtgc
     9661 cggctccggc gacgtcaagt accatcaggg cttctcttcc aatgtgatga catcggtcgg
     9721 acagacccat ctggcgctgc tgttcaaccc ttcgcacctg gagatcgtca ccccggtcgt
     9781 cgccggatcg gtgcgggcac ggcaggaccg ccgcaacgac ccgctgggca accaggtggt
     9841 gccggtggcg atccatggcg atgccgcttt tgccggccag ggggtggtga tggagacgct
     9901 gcagatgtcg caggtgcgtg gttaccgcat cggcggcacc attcacatca tcgtcaacaa
     9961 ccaggtcggt ttcaccacca gtcgggccga tgatgcccgc tccaccgagt actgcaccga
    10021 cgtggccaag atggtgcagg caccgatctt ccatgtgaat ggcgatgacc ccgaggcggt
    10081 gctgttcgtg acccagttgg cggtcgacta ccgcgaaacc ttcaggcgtg atgtggtgat
    10141 cgacatggtc tgttaccgcc gccgtggcca caacgaagcc gacgagcctt ccggcaccca
    10201 gccgctgatg tacgccaaga tccgcacgct gccgagcacg atgcagctct actccgacag
    10261 gctggtcaac agcggggtga tcagccgcga gctggtcgat gcctggatcg ccgactaccg
    10321 caaggcgctg gaggagggca ggcatgtcgc caagagtctg gtcaccgaac ccaatctcaa
    10381 gctgttcgtc gactggacac cctacctcgg ccatgactac tggtcggcca ccgccgacac
    10441 ccgtttcgaa ttgaggcagc tgcaacagct ggcggcggag gtctgcaagg tgcccgaggg
    10501 ggtggtggtg cagaaacagg tggacaagat catcgacgat cgccgcaaga tgatcgccgg
    10561 tgcactgccg ctgaactggg gttttgccga gacgatggcc tatgccaccc tgttgaccga
    10621 gggccatccg gtgcggctga tggggcagga cagcggtcgg ggcacctttt cgcaccggca
    10681 cgcggtgatt cataaccaga aagatggatc gccccatgtg ccgctggcga acattccggg
    10741 cgtgacggca cgcttcacgc tgcatgattc attgctgtcg gaagaggcag cgctggcctt
    10801 cgagtatggc tacgccacca ccaatccgcg ggcactggtg atctgggagg cgcagttcgg
    10861 cgacttcgtc aatggcgccc aggtggtgat cgaccagttc atcgccagtg gcgagaccaa
    10921 gtgggggcgt ctctgcggtc tggtgatgtt cctgccgcac ggctatgaag ggcaggggcc
    10981 cgagcactcc tcggcgcggc tggagcgctt catgcagctc tgtgccgagc acaacatcca
    11041 ggtctgcatt ccaacgacgc cggcacagat cttccatctg ctgcggcgac agatgctgcg
    11101 accactgcgc aagccgctga tcgtgatgac gcccaagagc ctgctgcggc acaagctggc
    11161 catctcgtcg ctggaggatc tggccgaggg cgagttcaag ctggtgattc cggaaagcgc
    11221 cccgctcgat gccagccagg tcaggcggat cgtgctgtgc agcggcaagg tctactacga
    11281 tctgatcgag gcgcgcgagg cggcgcagcg cgaggatgtc gcggtgatcc gcatcgagca
    11341 gttctacccc tttccacatg agcagctgca acaggcgatg gcccccttca tccaggccca
    11401 gcaggtgatc tggtgccagg aagagccgat gaatcaaggg gtctggtaca gcagccagca
    11461 ccacatgatt catgtcctgg gtctgctcaa tccaggctgg aagctcgact atgtcggccg
    11521 tgaggcgtcg gcggcgccgg cgaccggtta cctgagcctg catgtcgagc aacagcagcg
    11581 gctggtcaaa gaggccctgg ggctcgatcg attcaatgat ttgcactgat gacaggaaca
    11641 accatgatgc tggaaattaa agcgccgagt tttccggagt cgatctccga cggtactgtg
    11701 gccacttggc acaagaaggt gggcgaggcg gtgttgcgcg atgaagtgct cgtcgacatc
    11761 gaaaccgaca aggtggtgat ggaggtggtg gcaccggcgg atggccggtt gagcgaaatc
    11821 ctgaagccgg agggtgagac ggtgctcagc accgagctga tcggactgtt cgaggcaggc
    11881 agtgccctga ccgggacgca gcccagtgcc gaaccgccgg cagcggccgc cgcgcaatcg
    11941 cagcaaccgc tggtcaatcc tgccgcccgc cgactggtgc aggcgcatgg gttgacgccg
    12001 gagcagatcg ccgccaccgg caaggggggg cgtctcacca agggcgatgt gttgcagcat
    12061 ctcgaacagg gtcagccgcc ggcgcaaccg gtaccggctg tttctaccgc cacctcgccg
    12121 cgtgccgtcg aaccggctgt cgtggccgag tcgggtgcgc ggatcgaaaa gcgcgtgccg
    12181 atgacccggc tgcgcgccag cgtcgccaga cggctggtcg aggcgcagca gacggcggcg
    12241 atgctgacca ccttcaatga ggtcaacatg cagccggtga tggatctgcg caccaagtac
    12301 agggatcagt tcgagaagcg ccatggggtg cggctcggtt tcatgtcctt cttcgtgcgc
    12361 gcctcggtcg aggcattgaa gcggtttccg gcggtgaatg cctcgatcga cggcaatgac
    12421 atcgtctacc acggttacca ggacatcggc gtggcggtct cgaccgaccg cggactggtg
    12481 gtgccgatac tgcgcgatgc cgcacagatg agcctggcca ggatcgaggc gcaggtgcgc
    12541 gactatggcc tgaaggcgcg tgatggcaag ctgaccatcg aggagatgca gggcggtacc
    12601 ttcaccctct ccaatggcgg tgtttttggt tcgctgctgt cgacgccgat cctcaatccg
    12661 ccgcagaccg ccattctcgg catgcacaag gtgcaggagc gaccgatggc ggtcgatggc
    12721 aagatcgaga ttctgccgat gatgtatctg gcgctgagct acgaccatcg tctgatcgat
    12781 ggtcgcgagg cggtacagtt cctggtcgcc atcaaggatt ttctcgaaga tcccgcacgc
    12841 atcctgctgg atctgtaaag gcccgtgaca ggggatcaat caccaccacg ctggagaaaa
    12901 gctcatggca ggcaagtttg atgtgatcgt catcggcgcc ggtccggccg gctatgtggc
    12961 cgcgatcaag tgcgcgcagt tgggcctcag caccgcctgt gtcgagcagt ggcgtcgtga
    13021 tgggcagacc gtgctgggtg gcacctgcct caatgtcggc tgcattccct ccaaggcgct
    13081 gctcgaatcg gcccacaagt accgtgaggc gaaagagcaa ctcgccgacc atggcgtgct
    13141 ggccaaagag gtgtcgctcg atctggcgac catgatggcg cgcaaggaga agatcgtcgg
    13201 caatctcacg ggcggcatcc aggcgctgtt caagggcaac ggcgtcacca cgctggccgg
    13261 tcgcggccgg ctgctggcgg gcaagcgggt cgaggtgacc gatgccgctg gcgcggtcga
    13321 ggagtacgag gccgaacatg tcatcatcgc tgccggttcc gagccgattc cgatttcaca
    13381 ggcgccactg gatggtcacc atgtggtcga ctcctcggcc gcgctggcct ttgaagaggt
    13441 gccgaagcgg ctgggtgtga tcggtgccgg tgtcatcggt ctggagctgg gcagtctgtg
    13501 gagtgcgctg ggtgccgagg tggtgctgct cgaggcgcag gagcgcttcc tgccgatggt
    13561 cgatcagcgc atcgccaagg aggcgcagaa gctgctgacc cggcaagggc tcacgatcca
    13621 actgggtgcc cgggtgaccc acaccgaggt caaggagggc gaagtgctgg tcaggtacag
    13681 caacgccaag ggcgatcagg cgctgcgggt cgatcgcctg attgttgcgg tgggtcgccg
    13741 ggcgcagacg gccaacctgc tggcggccga ttgcggtgtc aacctcgatg aacggggctc
    13801 gatcttcgtc aacgaccact gccgcaccga cgtgccaggg gtctatgcga tcggcgacgt
    13861 cgtgcgtggt ccgatgctgg cgcacaaggg catggaggag ggcatcatgg tggccgagcg
    13921 catcgcgggt caactgagcc aggtcaacta cgactgcatc ccgtcggtga tctacaccca
    13981 gcccgaagtg gcctgggtgg gtgacaccga agaggtgctc aaggcgcgcg ccgaaccgta
    14041 caaggtcggc agtttcgcct ttgccgccaa tggtcgcgcc ttggccgcgc atcagtccga
    14101 agggttggtg cgggtgctgg cccatgccga gagcgatcgg gttcttggcg ttcacatcat
    14161 cggcccgcag gcgtcggagc tgatcgcgca ggcggtggtg gcgatggaat tctctgccag
    14221 tgccgaggat ctggcgttga cggtctttgc ccatcccact ctctccgagg cggttcacga
    14281 ggcggcattg tcagtcaatg gccaggcgat tcacgcagtg aaccgggcaa gaaagtagcc
    14341 acccggtggt cgatcacgga aattttcatt caaccagacg gacggaagta acgagatgaa
    14401 tctgcatgaa tatcaggcca agcagctgtt tgccgagtat ggcctgccgg tgtcgaaggg
    14461 ggtggtggcc gacagtgccg ccgccgcagt cgaggcggcc agcctcattg gcggcgaaca
    14521 gtgggtggtc aaggcgcagg tccatgccgg cgggcgcggc aaggcgggtg gcgtcaaact
    14581 ggtgcgcagc aaggccgaga tcgaacagtt tgcccagcag tggctcggca agcggctggt
    14641 gacctatcag accgatgccg atgggcagcc ggtcaaccag attctgatcg agaactgcac
    14701 cgacatcgac cgcgagctct atctgggtgc agtggtcgac cgtgcatcgc agcgggtcgt
    14761 cttcatggcg tcgagcgaag gtggggtcga gatcgaaaag gtggccgagc gcagcccgga
    14821 gaagatcctg caggcgatca tcgatccact ggtcggcgca cagccctacc aggcgcggga
    14881 gatcgccttc cagctcggcc tcaatgacaa gcaggtcaaa cagttcaccg acctgttcgt
    14941 gcgcctgagc cggctgttcc atgagtgcga tctggcactg ctggagatca acccgctggt
    15001 gatcacccaa cagggcgacc tgcactgtct cgatgccaag atcaacatcg atggcaatgc
    15061 actctaccgg cagccgaaac tgaaggcgct gcatgacccg agccaggagg atgcccgcga
    15121 gcgcgaggcc gcggcctggg agctcaacta tgtcgcgctg gagggcaaca tcggctgcat
    15181 ggtcaatggc gctggtctgg cgatgggcac catggacatc atcaagcacc atggcggcaa
    15241 tccggccaac ttcctcgatg tcggcggcgg tgcgaccaag gagcgggtca gcgcggcttt
    15301 caaactgatt ctgtccgatg aaaaggtcaa ggcggtgctg atcaacatct ttggcggcat
    15361 cgtgcgctgc gacctgatcg ccgaggg
//
LOCUS       DAORCQ010000054        15338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2464_length_15338_cov_6.048158, whole genome shotgun sequence.
ACCESSION   DAORCQ010000054 DAORCQ010000000
VERSION     DAORCQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15338
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_15338_cov_6.048158"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3309
                     /gene="hrpA"
                     /locus_tag="PKI23_07125"
     CDS             <1..3309
                     /gene="hrpA"
                     /locus_tag="PKI23_07125"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNN36746.1"
                     /translation="IIDEAHERSLNIDFLLGYLKRLLPRRPDLKLIITSATLDLDAFS
                     RHFDGAPILIVEGRSHPVEIRYRPRDERDETADPPQAIVEVLREIEAEEGGAPRGDVL
                     VFLSGEQEIRDCADHLRKALLRDTEILPLYARLSHAEQQRIFSPHPGRRVVLSTNVAE
                     TSLTVPGIRYVIDLGHARISRYSYRSKVQRLPIEPISQASARQRAGRCGRVAAGICYR
                     LYAEADLLGRPQFTEVEIRRTNLAAVILRMLSLSLGEIDEFPFIDPPDRRLIRDGFKL
                     LEELGAVDGRGTLTPIGRQLAQLPVDPRIGRMLLEATRENALRELLIIGSAISLQDPR
                     ERPVGKQQAADEKHRLYQHPDSDFITLCNLWDHLDGLRQSLTQSQFRNHCRRNYLSWL
                     RLREWRELHRQLHLAARALHLRENSSPADPDAIHRALLTGLLSHVGVKRPEGDYQGTR
                     NRRFSIFPGSPLAKKGPKWLMALELVETSKLYARTVARIDPAWIEPLAKGLLRRNHFE
                     PHWERKRGEVVAYEQVTLHGLPIVERRLVNYGPIDPVTARDIFIRAALVERDLGSHPA
                     FFKHNQALIAELEGLEAKSRSRDLLVNDEEIYRFYDERIPPDVYDRKRFDAWRGEAER
                     ARPDLLRLDRERLLRRDADEALGDRFPDQLQLGEMTLPLSYRFEPGHAEDGVTLHVPA
                     AAWRQLPRAQLEWLVPGLLRDKCIALLKSLPKAQRRHLVPLPDSVDRLLPALRPNAEP
                     LTSALTSALRSELGVTLADTDWTDGTIDDHYRMRVELLDEQNRVLFASRDPAALDAFV
                     ATLDRQRLLPLPAGSPWRRERVTSWDFDALPESVDTQQAGIPIKRFVRMIDHGDHIQL
                     ELGDDPVEARQSRVRGILRLYMLALAPTVHYLRKQVRFERERALTFAAIAGAHDLIDE
                     LIEAAFRAILLDGRPLPTDRASFEARVAADRNQVVTRAQELVRLVSEILACYRPLRLQ
                     LEKTASPALADSHRDIEQQLTALLAPGFITATPYHWLSEFPRYLKAIDHRLQRLQGQL
                     ARDAQSRRTVADLWQNYLDAKASGRADPQRLDECRWLIEELRVSLFAQPLGTRQPVSE
                     KRILERLRQARGGQ"
     gene            3355..3624
                     /locus_tag="PKI23_07130"
     CDS             3355..3624
                     /locus_tag="PKI23_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005475027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HNN36747.1"
                     /translation="MSDDEKNALLSRAAAAALLALGLAATSTAALAEGKGVEKCYGVV
                     KAGSNDCHTDSHSCASQAKKDADPQEWILLPAGSCEKIVGGVVKK"
     gene            3763..4395
                     /locus_tag="PKI23_07135"
     CDS             3763..4395
                     /locus_tag="PKI23_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36748.1"
                     /translation="MNLKTLCRAHLGVFLLALSSAAVCDAASVNPKIASHAVIPITQN
                     SYPKLYAAWGESGIKKINSLMLPAAEKIASSSECDRVDLVEISKSRSTPGGKIVFFVD
                     CVNRKRFYIEDSDLKSATAIQSQTAKMSGLADSQATQSCENAIKAQLHNPLTFNRKFG
                     TTSVYRAPTTGNVVVQFTFEAKNNLGGVLPSEARCVLTGRGIEEASISKN"
     gene            4743..5261
                     /gene="purE"
                     /locus_tag="PKI23_07140"
     CDS             4743..5261
                     /gene="purE"
                     /locus_tag="PKI23_07140"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNN36749.1"
                     /translation="MAGRRDRKAPLVGIIMGSQSDWETMGHAAEILTELAIPHEVEVV
                     SAHRTPDKLFAYAEAAAGRGLEVIIAGAGGAAHLPGMTAAKSALPVLGVPVQSRSLNG
                     VDSLLSIVQMPAGIPVGTLAIGRAGAINAGLLAAAILANKYPAIGTRLAAYRAAQTER
                     VLADPDPRASQS"
     gene            5258..6322
                     /locus_tag="PKI23_07145"
     CDS             5258..6322
                     /locus_tag="PKI23_07145"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNN36750.1"
                     /translation="MRVGIIGGGQLARMLALAGLPWAHQFVFLDPAADACAGPLGKLL
                     VADYDDERALLELAGQVDVVTFDFENVPARSLEVLAGRVPVHPGARALAAAQDRLHEK
                     TLFRELGMATAAFEVVDGAAGLPAAVVALGLPALLKTRRFGYDGKGQWLLRTAADVEV
                     AARELGDTPALLERLVPFEREVSLIAARGRDGAVACYPLAENRHRNGILHTTFCRTSD
                     PLQQEAEAQVGRLLEVLDHVGVLAVEFFVCDGALLANEFAPRVHNSGHWSIEGAETSQ
                     FENHLRAILGLPLGSTRARGDYAMLNLIGAVPDPARVLAVPGAHLHDYGKAPRPGRKV
                     GHITVACAVPGLLPPELLAL"
     gene            6408..7826
                     /locus_tag="PKI23_07150"
     CDS             6408..7826
                     /locus_tag="PKI23_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNN36751.1"
                     /translation="MTTASTSPTLTADVLASGSPLARRWRWACLLFILPQLLVGGHGL
                     LFSGLDWPWLTVPLVNGLFALYLLRESSRPLKLLEAIGQQSQHLHAGHPAQRLTAEPA
                     LASLNPLLEQFNRLGDALHALRESAARHPEELAPGSHAVQPTENDFLTDLQRSATRHL
                     RDNLRTVQEDLLSINQKLQQAAQLAGDNAELAIDSSTGIDALGQALQRIVTEVGSVEA
                     VVAELDDESQKVLKSLEMIAAIADQTSLLALNAAIEAARAGEQGRGFAVVADEVKALA
                     ERTKGATVEIEGSMGRFKGQVARITVESTRSGAAASEAVAKTRQFRDHFAALSTASRQ
                     MSGQIDHATDHAFATLVKVDHVLYKQNGYLAIAGQGNNEEATAVQVDHTGCRLGKWLQ
                     QDGQRLFGQTHAYRTLTAPHQAVHQHVRSAVEQARQPWAQDRARQTAIMADAEAAERA
                     SEEVMRLLGRMVDEKHDATARH"
     gene            complement(7867..10560)
                     /locus_tag="PKI23_07155"
     CDS             complement(7867..10560)
                     /locus_tag="PKI23_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNN36752.1"
                     /translation="MSIRNLDALFKPRSIALIGASNEAAKVGRVIADNLFNGGFSGPI
                     MPVNPHHPVVRGVISYADVASLPHTPDLAVVATPAATIPALAAELAARGTRALVVIST
                     GFGGAAGSPGAQLKQALLDAARPHCMRIVGPNCLGLLVPGSGLNASFAHMNARPGRLA
                     FLTQSGAIVTSLLDWAAVRGIGFSHLVSLGEMADVDFGDLLDYLANDPDTDAILLYIE
                     AVTAPRKFISAARAAARLKPLIVVKGGRHAEGARAAASHTGAMAGDDAVYEAIFRRTG
                     MLRVDDLEALFGAAEIVAMSPRITGDRLAILTNGGGMGVLATDALMDAGGTLAELAPA
                     TLAALDQLLPASWSRSNPVDIIGDAPGSRYAAAYQALAADPGIDALLALNCPTAVASS
                     LEAAQALIAARGTASQPLLFTSWVGAASMAEARAGFARARIPSYDTPEQAVRAFSYMV
                     DHAKARQLLLETPPSIPEQFTPDERKARRLIDAALAEGRQWLLEPEAKALFEAYAIAT
                     VPTRRAVTPAEAAAIAAQFAVPVALKVLSPDLPHKSDVGGVQLDLATPAAVQEAAAAM
                     LARLRKSQPQARIEGFCVEPMTRRPNAFELIIGAFVDPQFGPVILFGQGGTAVELIRD
                     RSLELPPLNMRLAHELINRTRLARLLRGYRDHPAVNLDAVALSLIKLAQMVTDLPELV
                     ELEINPLLADAEGVIALDARVRLRPSTLSASQRLAIRPYPKALESRVTLGDGRALLLR
                     PIRPEDEPALQAALLLLTPEQQRLRRLLARPELDERLAARLTQIDYDREMALVLTAAT
                     ALSDGEIFGVVRLMADPDNESAEFAIMLRPELSGQGVGRQLMERLIDHARDRGIGQLY
                     GDVLPQNQSMRRLCAALGFVEARSPLDDQLLRVSLPLRNPT"
     gene            complement(10588..11157)
                     /locus_tag="PKI23_07160"
     CDS             complement(10588..11157)
                     /locus_tag="PKI23_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate pyruvate-lyase family protein"
                     /protein_id="HNN36753.1"
                     /translation="MTLFNSKGWMPESTLSATDVTAVDFAALPPILRTLLVTDGTVTK
                     TLEAYFWEPIRIEQQQQQPVRSSTPMPLLEVAAGEPLWRRQVRLLGAHSGRCYALGLS
                     FLRLDVLPEPLQQALRAGRLGIGELLRESTLESYRRIVSIGHVAADHPADPGPGQWIE
                     RTYLIQTGGRPAIQVTELFPLALYRSLAN"
     gene            11255..11752
                     /locus_tag="PKI23_07165"
     CDS             11255..11752
                     /locus_tag="PKI23_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HNN36754.1"
                     /translation="MSTTLSLWRFAVDRYARPQVRQHCLQLQDMHGLSVNWLLWLLWL
                     EAQGRQATPALLDRAEADTGRLRRQLIEPLRQMRRSLDQQDEAPLRRQIGRVELTAER
                     LELRRLQRLRPLAVPITAVDNLHGYLHWLAPVDPAPIEPLLAALRLRVLADPTPSLQN
                     GAPPS"
     gene            11846..12601
                     /locus_tag="PKI23_07170"
     CDS             11846..12601
                     /locus_tag="PKI23_07170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNN36755.1"
                     /translation="MVKMARVHQPLGLILGFCLTTAALAAPTAAKGPNLDSDARKLSY
                     SVGISLGNRLRDDFTNLDMEALTAGLRHGLDGTKPALSEEEMQKIFADWSSKKTAADQ
                     QARESAAKKNSSEGEAFLKSHGKESGVTTLPSGIQYKVIKQGSGKSPTLEDSVVVHYR
                     GSLLDGTEFDSSRKRGAPATFPVRGLIPGWTEVLQKMKEGDQWEVVIPAELAYGDQGA
                     GRKIGPNAVLRFDMELLQVQGGEPGAASAPAAQ"
     gene            12740..15175
                     /locus_tag="PKI23_07175"
     CDS             12740..15175
                     /locus_tag="PKI23_07175"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN36756.1"
                     /translation="MSDSLSLPVALSAEQLFRRCDPAGLGFATTAELRPSGAPLGQQR
                     AIEAMAFALDVKRPGYNIFALGTPGSGRHAMIEQMLKGRAATEAVPGDTCHVHNFAQP
                     NKPIWLKLPCGMGVQLRDAMTQLVEDLHSAIRTAFESDEYRARRQVIEQEAMERREAV
                     IEQLREEAKAEDALLITLPTGFAFVPVRNGEMIKPDEFHQLPQELRDRYEERITALQK
                     RLQDALAQFPVWEREMRNRIKALNQAVTLSAVGLLIGELKQRFATLPAVLRHLDAVQD
                     DVVDNAQEFLGGGEEETANNPFAPQPTFTRYQVNLLVSHDNGGGAPVVYCDLPSQPNL
                     IGRVEYRAQYGMLSTDLTLIRPGALHQANGGYLMLDAAKVLTQPLAWEGLKRSLRAGE
                     IRIESLDRALGLSSTVTLEPEPIPLDLKVVLVGEPLLFTLLSQYDPEFLELFKVMADF
                     DDDLERSPDSERAIAAQIARIAGDEAMLPFEAAAVARIIEQGSRAIEDSQRISIRQDL
                     LHGLMVEADHCARQQGSSAVSAAAVERAITAQINRADRLRGRLQEQILRDTVRIDCSG
                     QRVGQINGLAVLSFGNFLFGHPSRITARVRPGSGGVLDIERKVELGGSLHSKGVLILS
                     SYLAAQYGLDGPLSLSASLVFEQSYGGVDGDSASSTELYALLSALAELPIRQSWAVTG
                     SVDQFGRVQAIGGVNEKIEGFFDICRARGLTSEQGVLIPASNVQHLMLRQEVVDACRN
                     GQFKIFAVETIDQGIALLTGVAAGQRDAEGRYPADSVNGRVEARLNRFAQQLQRKPQA
                     DAEADEADDGAGP"
BASE COUNT         2590 a         5178 c         5116 g         2454 t
ORIGIN      
        1 atcatcgacg aggcgcatga gcgcagcctc aacatcgact tcctgctcgg ctatctcaag
       61 cggctgctgc cccggcggcc cgatctgaag ctgatcatca cctcggccac cctcgacctc
      121 gacgccttct cgcgccactt cgacggcgcg cccatcctga tcgtcgaggg gcgcagccat
      181 ccggtggaga tccgctaccg cccgcgcgac gagcgtgacg agacggccga tccgccgcag
      241 gcgatcgtcg aagtcctgcg cgagatcgag gccgaggagg gcggcgcgcc acgcggcgat
      301 gtgctggtct ttctcagcgg cgagcaggag attcgcgact gcgccgacca cctgcgcaaa
      361 gcgctgctgc gcgacaccga gattctgccg ctctatgccc gcctctccca tgccgagcag
      421 cagcgcatct tcagcccgca ccccggccgg cgggtggtgc tctcgaccaa tgtcgccgaa
      481 acctcgctga cggtgcccgg catccgctat gtgatcgacc tcggccatgc ccgcatcagc
      541 cgctacagct accgcagcaa ggtgcagcgg ttgccgatcg aaccgatctc gcaggccagc
      601 gcccggcagc gggcgggccg ctgcggccgg gtggcggccg gcatctgcta ccggctctat
      661 gccgaagcgg atctgctcgg ccgtccccaa ttcaccgagg tggagattcg ccgcaccaac
      721 ctggccgcgg tgattctgcg catgttgtcg ctctctctcg gcgagatcga cgaatttccg
      781 ttcatcgacc cccccgatcg acggctgatc cgcgatggct tcaagctgct ggaagaactc
      841 ggcgccgtcg acggccgtgg cacgctgacg ccgatcggtc gccaactggc gcaactgccg
      901 gtcgacccgc gcatcggccg gatgctgctc gaggccacgc gcgagaacgc actgcgcgag
      961 ctgctgatca tcggcagcgc gatctcgctg caggacccgc gcgagcgccc ggtcggcaag
     1021 cagcaggccg ccgacgagaa gcatcggctc taccagcacc ccgactccga cttcatcacc
     1081 ctctgcaacc tgtgggacca tctcgacggg ctgcgccaga gcctgacgca gagtcagttc
     1141 cgcaaccact gccgtcgcaa ctacctgtcg tggttgcggc tgcgggagtg gcgcgagctg
     1201 caccgtcagc tccacctggc cgccagggca ctgcatctgc gcgaaaacag cagcccggcc
     1261 gaccctgacg ccatccaccg cgcactgctg accggcctgc tgagccatgt cggcgtcaag
     1321 cggccggagg gcgactatca gggcacccgc aatcgccgct tctcgatttt tcccggctcg
     1381 ccgctggcca agaaagggcc gaaatggctg atggcgctgg agctggtgga gacctcgaag
     1441 ctctatgccc gcaccgtggc ccgcattgac ccggcctgga tcgagccgct ggccaagggg
     1501 ctgctgcggc gcaaccactt cgaaccccac tgggagcgca agcggggcga ggtggtcgcc
     1561 tacgaacagg tcaccctcca tggcctgccg atcgtcgagc gccgactggt caactacggg
     1621 ccgatcgacc cggtcaccgc ccgcgacatc ttcatccgcg ccgcgctggt ggagcgcgac
     1681 ctcggcagcc atcccgcctt cttcaagcac aaccaggcac tgattgccga actggagggg
     1741 ctcgaagcca aatcgcgcag ccgcgacctg ctggtcaatg acgaggagat ctaccgcttc
     1801 tacgacgagc gcatcccgcc cgacgtctac gaccgcaagc ggttcgatgc ctggcgcggc
     1861 gaggccgaac gcgcccgacc cgacctgctg cggctcgacc gcgagcggct gctgcgccgc
     1921 gatgccgacg aggcgctcgg cgaccgcttt cccgaccagt tgcagctcgg cgagatgacg
     1981 ctgccgctga gctaccgctt cgaaccaggc catgccgaag atggcgtgac cctgcatgtg
     2041 ccggcggcgg cctggcgaca actgccgcgc gcgcagctcg aatggctggt gccggggctg
     2101 ctgcgcgaca agtgcatcgc cctgctcaag agcctgccca aggcgcagcg ccgccacctg
     2161 gtgccgctgc cggacagcgt cgaccggctg ctgccggcgc tgcgacccaa tgccgaaccg
     2221 ctgaccagcg cactgacctc ggccctgcgc agcgagctgg gcgtcaccct ggccgacacc
     2281 gactggacgg atggcaccat cgacgaccac taccggatgc gggtcgaact gctggatgaa
     2341 cagaaccggg tgctgttcgc cagccgtgat ccggccgcgc tcgatgcctt tgtcgccacg
     2401 ctcgaccgcc agcggctgct gccgctgccc gccggctcgc cgtggcgacg cgagcgggtc
     2461 accagttggg acttcgatgc actgccggag tcggtcgaca cccagcaggc tggcatcccg
     2521 atcaaacgct tcgtgcggat gatcgaccac ggcgaccaca tccagctcga actgggcgac
     2581 gacccggtcg aggcccgtca gagccgggtg cgcggcatcc tgcggctcta catgctggcg
     2641 ctggcgccga ccgtgcacta cctgcgcaaa caggtgcggt tcgagcgcga gcgggcgctc
     2701 actttcgccg cgatcgctgg cgcgcacgac ctgatcgacg aactgatcga ggccgccttt
     2761 cgcgccatcc tgctcgacgg ccgcccgctg ccaaccgacc gcgccagctt cgaggcgcgg
     2821 gtggcggccg accgcaacca ggtggtgacg cgggcccagg agctggtccg gctggtcagc
     2881 gaaattcttg cctgctaccg gccactgcgg ctgcaactcg aaaaaaccgc ctcgccggcg
     2941 ctggccgaca gccaccgcga catcgaacaa caactgacgg cgctgctggc accgggcttc
     3001 atcaccgcca ccccctacca ttggctgagt gaatttcccc gttacctcaa ggcgatcgac
     3061 caccggctgc aacggctgca gggacagttg gcccgcgacg cgcagtcacg ccgtaccgtg
     3121 gccgatctgt ggcagaatta cctcgacgcc aaggccagcg gccgggccga tcctcagcgg
     3181 ctcgacgaat gccgctggct gatcgaggag ctgcgggtct cactcttcgc ccagccactc
     3241 ggcacccgcc agccggtgtc ggaaaagcgc atcctcgaac ggctgcgcca ggcacgcggt
     3301 gggcaatagc catcctcttg atcagcaaca gaaaacaacc gaaggagcag aaccatgtcc
     3361 gatgatgaaa aaaacgccct gctgagccgc gccgctgcgg cagccctgct ggccctcggc
     3421 ctcgccgcca cttcgacagc agcgctggcc gaaggcaagg gcgtggaaaa gtgctatggc
     3481 gtggtcaagg ccggcagcaa cgactgccac accgacagcc acagttgcgc cagccaggcc
     3541 aaaaaggatg ccgacccgca ggagtggatc ctgctgcccg ccggcagttg cgaaaagatc
     3601 gtcggcggcg tggtcaagaa gtgactgagc gcgtttgagt ggcggcagct ctttcgacag
     3661 gtcgcgcgct ggcgcctgtc tgcccgggca ggcctgcgat gaaatggagc ggcggagaaa
     3721 atcgagcctg agcttatttt cctttttgcg gaggccactg ccttgaatct caaaaccctt
     3781 tgcagagcgc accttggagt ttttctactt gccctatcct cagccgctgt ttgtgacgct
     3841 gcttcagtca accccaagat cgcctcgcac gcggtcatcc caatcacgca aaatagctac
     3901 ccgaagttat acgccgcctg gggtgagtcg ggcatcaaga aaatcaactc cctcatgctc
     3961 cccgcagcag agaaaattgc ctcatcatcc gagtgcgaca gggttgatct cgtcgagatc
     4021 tcgaaaagcc gaagcacgcc gggcggcaag atcgtcttct ttgttgattg cgtgaatcgg
     4081 aaacgcttct acattgaaga ctccgatctt aaatcagcca ccgctattca gtctcaaact
     4141 gccaagatgt ctggcttggc agactctcaa gccacgcaaa gctgcgagaa cgcgatcaaa
     4201 gcccagcttc acaacccgct caccttcaat cggaagttcg gcacgacgtc tgtttatcgt
     4261 gcacccacga ccggcaacgt cgtcgtccag ttcaccttcg aagccaagaa caatcttggt
     4321 ggcgttctac ccagtgaggc gcgttgcgtg cttacaggcc gcggcatcga ggaagcttca
     4381 atctcaaaga actaacgaca cgttgatttt atccacgtga aatcggggac atgaaatcgg
     4441 cggctcattg ttgaaggcag tccgaagcag gccgtccggt cagcgcagcg gttcactgtg
     4501 gtggctgttt gagcagcagg cgcctgactt cgcgcaaggc gcgcgctaga atcgcggctg
     4561 gcagcgccgg ggcaggcact gcggagccac tccgcagttg cgccgtccgg ggcaaggttc
     4621 actcaccggc cgagcccagg gctccggcac cccgcggaca cattcatgca cagtgcgcag
     4681 aatctggtcg tcgcaccggt tgtcgccgac atttcccttt tcttcagtgc gaggactcgg
     4741 tgatggccgg tcgccgtgac cgcaaggcgc ctctggtggg gatcatcatg ggctcgcagt
     4801 cggactggga gacgatgggc cacgccgccg agattctgac cgagcttgcc attccccacg
     4861 aggtggaggt ggtctcggcc catcgcacgc cggacaagct gttcgcctat gccgaggccg
     4921 ccgccgggcg cgggctggag gtgatcatcg ccggcgccgg cggcgccgcc cacctgccgg
     4981 gcatgaccgc cgccaagagc gcgctgccgg tgctgggcgt gccggtgcag tcgcgcagcc
     5041 tcaatggcgt cgattcactg ctctccatcg tgcagatgcc ggccggcatt ccggtcggca
     5101 cgctggccat cggtcgcgcc ggcgccatca acgcgggcct gctggctgcg gccattctcg
     5161 ccaacaaata tcctgccatc ggcacgcgcc tggcggccta tcgcgcggcc cagaccgaac
     5221 gggtgctggc cgatcccgat ccgcgggcga gccagtcgtg agggtcggca tcatcggtgg
     5281 cgggcagctg gcgcggatgc tggcgctggc cgggctgccc tgggcgcacc aatttgtgtt
     5341 tctcgacccg gccgccgacg cctgcgccgg gcccttgggc aaactgctgg tcgccgatta
     5401 cgacgatgag cgcgcactgc tcgaactggc tgggcaggtc gatgtggtga ccttcgattt
     5461 cgagaacgtc ccggcacgca gcctggaggt gctcgccggc cgggtgccgg tccatcccgg
     5521 tgcgcgggcg ctggcggcgg cgcaggaccg gctgcacgag aagacgctgt tccgcgagct
     5581 cggcatggca acggcggcgt tcgaggtggt cgatggcgct gccgggttgc cggcagccgt
     5641 ggtggcactg gggctgcccg cgctgctcaa gacccgccgg ttcggctatg acggcaaggg
     5701 ccagtggctg ctgcgcacgg ctgccgatgt cgaagtcgcg gcccgtgaac tgggtgacac
     5761 accggcattg ctggagcggc tggtgccgtt cgagcgcgag gtgtcgctga tcgcggcgcg
     5821 cggccgcgat ggcgccgtgg cctgttatcc gctggcggaa aaccgccacc gcaacggcat
     5881 cctccacacc accttctgcc gcaccagcga tccgctgcaa caggaggccg aagcccaggt
     5941 cggacgcctg ctcgaagtgc tcgaccatgt cggcgtgctg gccgtggagt tctttgtctg
     6001 cgacggggcg ctgctggcca acgaatttgc accgcgggtg cacaactccg ggcactggag
     6061 catcgagggt gccgagacca gccagttcga aaaccacctg cgggcgattc tcggcctgcc
     6121 gctcggcagc acccgcgcgc gcggcgacta cgcgatgctc aatctgatcg gcgcggtgcc
     6181 cgaccctgca cgggtgctgg cggttcccgg cgcccatttg catgattacg gcaaagcacc
     6241 acggccgggg cgcaaggtgg gccacatcac cgtcgcctgc gccgtgccgg gcctgctgcc
     6301 gccggagttg ctggcactct gaccgccgtt gcgcgccact ctggcatccg ttgtctacac
     6361 ttcactcaac gcaatgacca cccctctttt gccgtggagt gaccccgatg acgacagctt
     6421 caacctcgcc gaccctgact gcggatgttc tcgccagcgg cagcccgctg gcccgccgct
     6481 ggcgctgggc ctgcctgctg ttcattctgc cgcaactgct ggtgggcggt catgggctgc
     6541 tgttttccgg gctcgattgg ccgtggctga cggtgccgct ggtcaatggc ctgtttgccc
     6601 tctatctgct gcgtgagagc agccggccac tgaagctgct ggaggccatc ggccagcagt
     6661 cgcagcatct gcacgccggt catccggctc agcggttgac tgccgagccg gcactggcca
     6721 gcctgaatcc gctgctggag cagttcaaca ggctgggtga tgcactgcac gcgctgcgcg
     6781 agtcggcggc ccgtcacccg gaagagctgg ctcccggcag tcacgctgtg caaccaacag
     6841 aaaacgattt tctgaccgac ctgcaacgca gcgcgacccg tcacctgcgc gacaacctgc
     6901 gcacggtgca ggaagacctg ctgtcgatca atcagaagct gcaacaggcg gcacagctcg
     6961 ccggcgacaa cgccgagctg gcgatcgaca gttcgaccgg catcgatgcg ctgggccagg
     7021 cactgcaacg catcgtcacc gaggtcggtt cggtcgaggc ggtggtggcc gaactcgacg
     7081 atgagagcca gaaggtgctg aagtcgctgg agatgatcgc ggcgattgcc gatcagacct
     7141 cgctgctggc gctcaatgcc gcgatcgagg cggcccgtgc cggcgagcag gggcgcggct
     7201 ttgcggtggt ggccgatgag gtgaaggcgc tggcggagcg gaccaagggg gccacggtcg
     7261 agatcgaggg cagcatgggc cgcttcaagg ggcaggtggc ccgcatcacc gtcgagtcga
     7321 cccgcagcgg ggccgccgcc agcgaggcag tggcgaagac ccgccagttc cgcgaccatt
     7381 tcgcagccct gtcgaccgcc tcacggcaga tgagcgggca gatcgaccat gccaccgacc
     7441 acgcctttgc cacgctggtg aaggtggacc atgtgctgta caagcagaat ggctacctgg
     7501 cgattgccgg ccagggcaac aacgaggagg cgacggcggt gcaggtcgat cataccggct
     7561 gccggctggg caagtggctg cagcaggatg gccagcgcct gtttggccag acccacgcct
     7621 accggacgct gacggcgccg catcaggcgg tgcatcagca tgtgcgcagt gcagtggagc
     7681 aggccaggca gccgtgggcg caggatcgtg cgcggcaaac ggccatcatg gccgatgccg
     7741 aggccgcaga aagggccagt gaggaggtga tgcggctgct cggtcgcatg gtcgatgaga
     7801 agcatgacgc caccgctcga cactgaggcc ggccagcacg cctgaaccgg atcgaccggt
     7861 ggcgattcag gtcgggttgc gcagcggcag gctcacccgc agcagctggt catcgagcgg
     7921 ggagcgcgcc tcgacgaagc cgagtgcggc acagagccgg cgcatcgact gattctgtgg
     7981 cagcacatca ccgtagagct ggccgatgcc gcggtcgcgg gcatggtcga tcagccgctc
     8041 catcagttgc cgcccaaccc cctgtccgct caactccggc cgcagcatga tggcgaattc
     8101 ggccgattcg ttgtccggat cggccatcaa ccgcaccacg ccgaagatct cgccatcgct
     8161 cagggcggtg gccgcggtga gcaccagcgc catctcacgg tcgtagtcga tctgggtcag
     8221 ccgcgccgcc agccgctcgt cgagttcggg ccgcgccagc agtcgccgca gccgctgctg
     8281 ttcgggcgtc aacagcagca gtgccgcctg cagtgccggt tcatcctcgg gacgaatcgg
     8341 ccgcagcagc agtgcgcgtc catcaccgag tgtgacccgg ctttcgagcg ccttgggata
     8401 ggggcgaatc gccagccgtt gcgaagcgct cagcgtgctc ggccgcagcc gcacccgggc
     8461 atcgagcgcg atcaccccct cggcatcggc cagcagcggg ttgatctcca gttcgaccag
     8521 ttccggcagg tcggtcacca tctgcgccag cttgatcagg ctcagcgcca ccgcgtcgag
     8581 gttgaccgcc ggatggtcgc ggtagccgcg cagcagccgc gccagccggg tccggttgat
     8641 cagttcatgg gccaggcgca tgttgagcgg gggcagttcg agcgagcggt cgcggatcag
     8701 ctcgaccgcc gtgccgccct gaccgaacag gatcaccggc ccgaactgcg gatcgacgaa
     8761 ggcgccgatg atcagctcga aggcgttggg ccgtcgggtc atcggctcga cgcagaagcc
     8821 ttcgatgcgc gcctgcggct ggctttttcg caaccgggcc agcatcgctg cggctgcctc
     8881 ctgcaccgcc gctggcgtgg ccagatcgag ctgcacgccg ccgacatccg acttgtgcgg
     8941 cagatcgggc gacagcacct tcaacgcgac cggcacggca aattgggcgg cgatggccgc
     9001 cgcctcggcg ggcgtgacgg cgcgccgggt cggcaccgtc gcaatcgcat aggcttcgaa
     9061 cagtgccttg gcttcgggtt cgagcagcca ctgccgtccc tcggccagcg ccgcatcgat
     9121 cagccggcgc gccttccgct cgtcgggggt gaactgctcg ggaatcgatg gcggagtctc
     9181 gagcagcagc tgccgcgcct tggcgtggtc gaccatgtaa ctgaaggcac gcaccgcctg
     9241 ctccggcgtg tcgtagctgg ggatgcgcgc ccgggcgaaa ccggcgcggg cttcggccat
     9301 cgaggcggcg ccgacccagc tggtgaacag cagcggctgg ctggccgtgc cgcgcgccgc
     9361 gatcagcgcc tgggcggcct cgaggctcga tgccaccgcc gtcgggcagt tcagcgccaa
     9421 gagcgcatcg atgccggggt cggcagccag cgcctggtag gccgcggcat aacgactgcc
     9481 cggtgcgtcg ccgatgatgt cgaccgggtt gctccgtgac caactggccg gcagcagctg
     9541 atcgagcgcg gccagcgtcg cgggtgccag ctcggccagt gtgccgccgg catccatcag
     9601 cgcatcggtc gccagcaccc ccatgccgcc accattggtg aggatcgcca gccggtcgcc
     9661 ggtgatccgt ggcgacatcg ccacgatctc ggcagcgccg aacagcgctt cgaggtcatc
     9721 gacccgcaac atgccggtgc ggcgaaagat cgcctcgtag accgcatcat cgccggccat
     9781 cgcgccagtg tgcgaggccg ccgcgcgtgc gccctcggca tggcgcccac ccttcacgac
     9841 gatcagcggc ttgagccgcg cggccgcgcg cgccgccgag atgaacttgc gcggggcggt
     9901 gacggcctcg atgtagagca ggatcgcgtc ggtgtcgggg tcgttggcga ggtaatcgag
     9961 caggtcaccg aaatcgacat cggccatctc gcccagcgag accaggtgcg aaaagccgat
    10021 gccacgcacg gcggcccagt cgagcagcga ggtgacgatg gcgcccgact gggtcagaaa
    10081 ggccagccgg cccggccgcg cattcatgtg ggcgaagctg gcgttgaggc cgctgcccgg
    10141 caccagcagc ccgaggcagt tgggtccgac gatgcgcatg cagtgcggtc gcgcggcgtc
    10201 gagcagcgcc tgcttgagct gggcgccagg cgagccggcc gcaccaccga aaccggtcga
    10261 gatcaccacc agcgcgcggg tgccacgcgc cgccagttcg gcggccaggg ccgggatcgt
    10321 cgctgccggc gtggccacca ccgccagatc gggcgtgtgg ggcaggctgg cgacatcggc
    10381 atagctgatc acgccccgca ccaccggatg gtgcgggttg accggcatga tcggtccact
    10441 gaagccaccg ttgaagaggt tgtcggcaat gacccgcccg accttggcgg cttcgttgct
    10501 ggcaccgatc agcgcgatcg aacgcggttt gaacagtgca tcgagattgc ggatcgacat
    10561 cgcacgctcc ttgaacagcg gctgtgctca gttggcgagt gaccgataga gcgccagcgg
    10621 aaacagctcg gtcacctgga tggccggccg gccgccggtc tggatcaggt aggtgcgctc
    10681 gatccactgg ccgggaccgg ggtcggccgg gtgatcggcc gcgacatggc cgatcgagac
    10741 gatgcgccgg tagctctcca gcgtgctctc gcgcagcagt tccccgatgc cgagccgccc
    10801 tgcgcgcagc gcctgctgca gtggctccgg cagcacgtcg agccgcagga aggagagccc
    10861 cagcgcatag caacgtccgc tgtgggcccc cagcagccgc acctgccgcc gccacagcgg
    10921 ctcaccggca gccacttcca gcagcggcat cggcgtgctg ctccgtaccg gctgttgctg
    10981 ctgctgttcg atccggatcg gctcccagaa ataggcttcg agggtcttgg tcaccgtgcc
    11041 gtcggtgacc agcagcgtgc gcaggatcgg tggcagcgcg gcaaaatcga ctgcggtgac
    11101 atccgtggcc gaaagggtgc tctccggcat ccagcccttg ctgttgaaaa gcgtcatgtc
    11161 ggctcgcatc actgaaaaag agggcggtgt cgctgcggga ttgtatcatc tgcccacgcc
    11221 actcctgttt cgcagacgag cctcgcgggc gccgatgagc acgacccttt cactctggcg
    11281 cttcgccgtc gaccgttatg cccggccgca ggtgcggcag cactgcctgc agttgcagga
    11341 tatgcatggg ctgtcggtca actggctgct ctggctgctc tggctcgaag cgcagggccg
    11401 gcaggcgacg ccagcgctgc tcgatcgggc cgaggccgac accggccggc tgcgccgcca
    11461 gctcatcgaa ccgctgcggc agatgcgacg cagcctcgac cagcaggacg aggcgccgct
    11521 cagacggcag atcggtcggg tcgagctgac tgccgagcgg ctggagctgc gccggttgca
    11581 gcggttgcgg ccgctggccg tgccgatcac tgcggtcgac aacctgcatg gctatctgca
    11641 ctggctggcg ccggtcgatc cggcaccgat cgagccgctg ctggcggcat tgcggctgcg
    11701 ggtgctggcg gaccccacgc cttcgctgca aaacggagcg ccaccgtcgt gaatacgcaa
    11761 ctctgcatta gcgggtagaa tggcccttca ctcgcttgac cacgcctttt caatgcgttt
    11821 ttttcaatcc ttcgaggaat cattcatggt gaagatggcc agggtacatc agccgctggg
    11881 tctgatattg gggttctgcc tgaccacggc ggcgctggct gcgcccacgg ctgccaaggg
    11941 acccaatctg gacagcgatg ccagaaagtt gagctactcc gtcggcatca gcctgggcaa
    12001 ccggttgcgt gatgacttca ccaacctcga catggaggca ctcaccgccg gcctgcgcca
    12061 tggcctcgat ggcaccaagc cggcgctgag cgaggaggag atgcagaaga tcttcgccga
    12121 ctggagcagc aagaagacgg cggccgatca gcaggcgcgc gagagtgccg cgaagaagaa
    12181 cagcagcgag ggcgaagcct tcctcaagag ccatggcaag gagagtggcg tcaccaccct
    12241 gcccagcggc atccagtaca aggtgatcaa gcagggcagt ggcaagagcc cgaccctgga
    12301 ggacagtgtc gtcgtacact accgtggcag cctgctcgat ggcaccgaat tcgacagctc
    12361 ccgcaagcgg ggcgcgccgg ccaccttccc ggttcgcggg ctgattccgg gctggaccga
    12421 ggtgctgcag aagatgaaag agggtgacca gtgggaggtg gtcattcccg ccgagctggc
    12481 ctatggcgac cagggcgctg gccgcaagat cggtcccaat gccgtgctgc gcttcgacat
    12541 ggagctgctg caggtccagg gcggtgagcc gggtgccgca tccgcgcccg ccgcgcagtg
    12601 agcggtgcgc tgcctttcat tccaatagac cggaggtgcc gactgccccg tcgcggcggg
    12661 cagtcggtgg caatctccgg cgctcatctc ttccgctgcg gcgcagtgtt gtctgacagc
    12721 aacaacagga atggccgtga tgtccgattc gctttccctt cccgtcgcac tctctgccga
    12781 gcagctgttc cgtcgctgtg atccggccgg actcggcttc gccaccaccg ccgaactgcg
    12841 gccgtccggc gccccgctgg gccagcagcg cgccatcgag gcgatggcct tcgcgctcga
    12901 tgtcaaacgg cccggttaca acatcttcgc gctcgggacc cccggttccg ggcgtcacgc
    12961 gatgatcgag cagatgctga agggacgcgc ggcgaccgaa gcggtgccgg gtgacacctg
    13021 ccatgtgcac aactttgccc agcccaacaa gccgatctgg ctcaagctgc cctgtggcat
    13081 gggggtgcag ttgcgcgatg cgatgacgca actggtggag gatctgcaca gcgcgatccg
    13141 caccgccttc gagagcgacg agtaccgcgc ccgccggcag gtgatcgagc aggaggcgat
    13201 ggagcggcgc gaggcggtga tcgagcagtt gcgtgaagag gccaaggccg aggatgcact
    13261 gttgatcacg ctgccgaccg gctttgcctt cgtgccggtg cgcaatggcg agatgatcaa
    13321 gccggatgag tttcatcaac tgccacagga gctgcgcgac cgttatgagg agcgcatcac
    13381 cgccctgcag aagcggctgc aggatgcgct ggcacagttt ccggtctggg aacgggagat
    13441 gcgcaaccgc atcaaggcgc tgaatcaggc ggtgaccctc tcggccgtcg ggctgctgat
    13501 cggcgagttg aagcagcgct ttgccacgct gccggccgtg ctgcgccatc tcgatgccgt
    13561 gcaggacgac gtcgtcgaca acgcacagga gtttctcggt ggcggtgaag aggagacggc
    13621 gaacaacccc tttgcgcccc agcccacctt cacccgctat caggtcaacc tgctggtgtc
    13681 gcatgacaat gggggtggcg ctccggtggt ctactgcgac ctcccctccc agcccaacct
    13741 gatcggccgg gtcgaataca gggcgcagta cgggatgctc tccaccgacc tgacactgat
    13801 tcgccccggc gcgctgcatc aggccaatgg cggctacctg atgctcgatg ccgccaaggt
    13861 gctgacccag ccgctggcct gggagggtct caagcgcagc ctgcgtgccg gtgagatccg
    13921 catcgagtcg ctcgaccgcg cgctggggct gagcagcacg gtgacgctgg agccggaacc
    13981 gattccgctc gacctgaagg tggtgctggt gggtgagccg ctgctgttca cgctgctgtc
    14041 gcagtacgac ccggagtttc tggaactgtt caaggtgatg gccgatttcg atgacgacct
    14101 cgaacgcagc ccggacagcg aacgcgccat cgccgcacag atcgcccgca tcgccggtga
    14161 cgaggcgatg ctgccgttcg aggcggcggc ggtggcgcgg atcatcgaac aggggtcgcg
    14221 ggccatcgaa gacagccagc gcatctcgat ccggcaggat ctgctgcatg gcctgatggt
    14281 cgaggccgac cactgtgccc gccagcaggg cagcagtgcc gtgtcggcag cggcggtcga
    14341 gcgtgccatc accgcccaga tcaaccgcgc cgaccggctg cgcggccgcc tgcaggagca
    14401 gattctgcgc gacaccgtcc gcatcgattg cagtggccag cgggtcggcc agatcaatgg
    14461 cctggcggtg ctcagcttcg gcaacttcct cttcggccat cccagtcgca tcaccgcccg
    14521 cgtccgcccc ggcagcggcg gcgtgctcga catcgaacgc aaggtcgaac tgggcgggtc
    14581 gctccactcc aagggggtgc tgattctctc cagctacctc gctgcgcagt atggactcga
    14641 tggcccgctg tcgctctccg ccagcctggt gttcgagcag tcctatggcg gcgtcgacgg
    14701 cgacagcgcc tcatcgaccg aactctatgc gctgctgtcg gcgctggccg agctgccgat
    14761 caggcaatcc tgggccgtca ccggctcggt cgaccagttc ggccgggtgc aggcgatcgg
    14821 cggtgtcaac gagaagatcg aaggcttctt cgacatctgc cgcgcgcgcg gcctgaccag
    14881 cgagcagggg gtgctgatcc cggccagcaa cgtccagcat ctgatgctgc ggcaggaggt
    14941 ggtcgatgcc tgccgcaacg ggcagttcaa gatcttcgcg gtcgagacca tcgaccaggg
    15001 catcgcgctg ctgaccggcg tcgcggcagg tcagcgtgat gccgagggcc gttaccctgc
    15061 cgactcggtc aatggccgtg tcgaggcgcg gctgaatcgc ttcgcccaac agttgcagcg
    15121 caagccgcaa gcggatgccg aggccgatga ggccgacgat ggagccgggc catgaaccgc
    15181 cagcggccgc gcacgctgct gttcgccatc gatctgtcgc cacccagcct gccgctgctc
    15241 gatgccgccg ccgagctggc ggcaatgctg gaagtcgagc tggcggtgtg gcggatcgag
    15301 gagcagcggc tgctggaagt gctcaaactc ccctggtg
//
LOCUS       DAORCQ010000055        15168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2503_length_15168_cov_5.701978, whole genome shotgun sequence.
ACCESSION   DAORCQ010000055 DAORCQ010000000
VERSION     DAORCQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15168
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_15168_cov_5.701978"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PKI23_07180"
     CDS             <1..673
                     /locus_tag="PKI23_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNN36757.1"
                     /translation="RRYLNRGLSLLDVIEEGNLGLIRAVEKFDPERGFRFSTYATWWI
                     RQTIERAIMSQTRTIRLPIHIVKELNVYLRAARELTKHLDHDPTADDVAKLLDKPVDD
                     VTRMFKLNERITSIDVPVAADSDRSVLDTLPDLHLPDPVETLQQNDLHANLGKWLDQL
                     SDKQAEVLSRRFGLRGYEMTTLEDVGKEIGLTRERVRQIQVEALRRLREIMEENGLSS
                     EAVFS"
     gene            766..1524
                     /locus_tag="PKI23_07185"
     CDS             766..1524
                     /locus_tag="PKI23_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN36758.1"
                     /translation="MSIDQPRFSACFMGTGPAGGSELGSSACVIEESGQPQLLIDCGP
                     DTLLRYEACYQRLPEALFITHCHFDHIGGLETLFYRATFAPEPREIRLFVPVTLLTTL
                     HHRLAAGVSCLAEGGVNFWDSFRLIPVDTGFWLKHNHYRVFPARHHLPDSAFGLALPG
                     QFFYSGDTRPIPEQLLLHASQGEVILHDARVTGNPSHSGLDDLLREYDAAQRGRMVIY
                     HHLPGDEPLFESAGFAVASPFRKITLGAGHVRQT"
     gene            complement(1545..1621)
                     /locus_tag="PKI23_07190"
     tRNA            complement(1545..1621)
                     /locus_tag="PKI23_07190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1585..1587),aa:Pro,seq:ggg)
     gene            1739..2884
                     /locus_tag="PKI23_07195"
     CDS             1739..2884
                     /locus_tag="PKI23_07195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNN36759.1"
                     /translation="MNNRPDHQLDPPVLFIEQPNRHGGKIGHAILNAERTLNALSLPM
                     IELLDQQLRRWAEDPELILVVLKGAGQRAFCAGGDVVELHRSMIAHPDGPNPYAEAFF
                     SREYRLDHLLHRYPKPLLCWGSGIVMGGGWGLMAGCSHRVVTPDSRLAMPEIRIGLYP
                     DAGASWFLGQLPRQISLFLGLTGAPLNASDARLLGVADHLLSRENQVEVEQRLQQTQW
                     SQESTSNHQHLSALLAEIAGKQSAQPPESATEKHQSLIHETCAATELAAVVEAILELP
                     QHDPWLAGAVKNLREGSMISALLTWHQLREQPPASLAGAFRQELVLSLQCACQHEFTE
                     GVRARLVERDQRPKWRYPSIRALPDRLLESFIEAPWPDGGHPLADLN"
     gene            3344..4312
                     /gene="cysK"
                     /locus_tag="PKI23_07200"
     CDS             3344..4312
                     /gene="cysK"
                     /locus_tag="PKI23_07200"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNN36760.1"
                     /translation="MANLFADNSLSIGNTPMVKLNRVTQGSVYAKIEGRNPAYSVKCR
                     IGASMVWDAEKSGRLKPGMELVEPTSGNTGIALAYVAAARGYGLTLTMPSSFSLERRK
                     IMRALGAKLILTDPSKGMNGAIVKAQEIAQSNPDRYLLLQQFENPANPAIHEQTTGPE
                     IWRDTDGKIDILVAGVGTGGTLTGVSRYIKQTQGKPITTVAVEPLDSSVIKNTLEGKA
                     PAHKPHKIQGIGAGFVPKNLDLGLVDQVEQVSNDEAIAFAHRLMREEGILAGISCGAA
                     AAAAARVAAQPQHQGKCIVVILPDSAERYLSSALFEGLFTEAELQQ"
     gene            complement(4356..4604)
                     /locus_tag="PKI23_07205"
     CDS             complement(4356..4604)
                     /locus_tag="PKI23_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNN36761.1"
                     /translation="MDKHDLIHELPEYREAIHRLKMNDAHFARLFERYHDVDREVRRI
                     ETGIEVSSDGYIEGLKRERLWLKDQLYGLLQAQAKTAE"
     gene            complement(4771..5025)
                     /locus_tag="PKI23_07210"
     CDS             complement(4771..5025)
                     /locus_tag="PKI23_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36762.1"
                     /translation="MAATFLEIVELADGEVVLQRSDGAGDPLIQLRFSDEAISYLNSS
                     HVEIAKAMIQAGIQLIGRAHSFSAHLDQGADDDLEPRTLH"
     gene            complement(5096..5626)
                     /locus_tag="PKI23_07215"
     CDS             complement(5096..5626)
                     /locus_tag="PKI23_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36763.1"
                     /translation="MNDALPRRVGQKLHFARIHLRELEKMAGLSATDLLSRRVAYSES
                     FAFHLTGAVRCHLIEVAGFYKIDPLPAPDLESMLSLLAERCGASPEISELRILASRGD
                     SWLKRLAVFYHACSEGIGVNSEPARNGLESHMTLLDLQELAEQRAVLPTVAECASWLS
                     AAMELIERQRQGMAQW"
     gene            complement(5766..6122)
                     /locus_tag="PKI23_07220"
     CDS             complement(5766..6122)
                     /locus_tag="PKI23_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNN36764.1"
                     /translation="MLEPSQNNELPVIPGKKYFTIGEVSELCDVKPHVLRYWEQEFPQ
                     LNPVKRRGNRRYYQRQEVLLIRQIRSLLYDQGYTIGGARQRLSGDEAKHEETQYRQLV
                     RQMIAELEQLLLQLKE"
     gene            complement(6097..6411)
                     /gene="ihfA"
                     /locus_tag="PKI23_07225"
     CDS             complement(6097..6411)
                     /gene="ihfA"
                     /locus_tag="PKI23_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNN36765.1"
                     /translation="MESRVLTKAEMAERLFEEVGLNKREAKEIVELFFEEIKLCLESN
                     EHVKLSGFGNFELRDKNRRPGRNPKTGEEIPITARRVVTFRPGQKLKARVENYVGTKP
                     KQ"
     gene            complement(6418..8802)
                     /gene="pheT"
                     /locus_tag="PKI23_07230"
     CDS             complement(6418..8802)
                     /gene="pheT"
                     /locus_tag="PKI23_07230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNN36766.1"
                     /translation="MKFSEAWLREWIDPPFATAELSARLTMAGLEVDGQSAAAPALSG
                     VVVAEIIAVQPHPNADRLRLCRVNAGERGELEVVCGAPNVRVGLRVPLVLAGARLPDG
                     SEIVATTVRGVHSAGMLAAASELGLDLAGEGLLELDAQAPLGAALSDVLQLDDQIIEI
                     GLTPNRGDCLGIAGLAREVGVLARQAVCAPAERTVPALSERVIPVRVEAPEACPRYLG
                     RVIENIQPDCKTPLWLRERLRRSGVRSISPVVDVTNYVMLELGQPLHAFDLEKIQGGI
                     QVRMARAGESITLLDGQQLQLDQEVLLIADAVKPLAMAGIMGGADSGVTATTRHLFIE
                     SAFFAPLAIAGRARRHGLHTDASHRYERGVDFELPRRALERATALLTGIVGGVPGPII
                     EQVQPELLPKPAVIALRASRINRLLGMEMAPTEVEDLLGRLGCRIESEAAGQWCVTVP
                     SFRFDLNIEVDLIEELARLHGYDRLPTRVPLMPLQPGAGSERTLSLREFRRRLTARGY
                     QEAITYSFVDPELNRLIDPAHEALPLANPMSKEMAVMRGSLWPGLLLALRGNLNRQQS
                     RVRLFESGLRFVQQGDQLLQEPMLAGVIAGSRHPESWGITNSEMVDFFDLKGDIETLF
                     ALTGARHQLELQPAHHPALHPGQSAQLVRDGKVLGQMGQLHPTLVDSFDLKSVPYLFE
                     LNLNLALENHLEAYRSWSHYPSVRRDLAFLVDRELPFRMLEQAIKAAAGPDLSQLILF
                     DLYQGKGIDPKRKSMALGLIFQSQSHTLGEAEIQSSLDAVIRALEQRFQAEMRR"
     gene            complement(8854..9870)
                     /gene="pheS"
                     /locus_tag="PKI23_07235"
     CDS             complement(8854..9870)
                     /gene="pheS"
                     /locus_tag="PKI23_07235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNN36767.1"
                     /translation="MESLEKLLHDAEAAIADSADLMALDQVRVFYLGKKGVITQQLKE
                     LGALPAEERRAAGALVNRVKEQLQALLAQRKQTLDAQALDQQLAQGWVDVTLPGRGQS
                     SGALHPITKVLERMEDIFTSIGYEVAEGPEVEDDYHNFEALNIPAHHPARAMHDTFYI
                     DAHRLLRTHTSPVQIRQMKQGQPPFRLICPGRVYRCDSDVTHTPMFHQVEGLVVDRDI
                     SFADLKGTVSGFLRSFFERDLQVRFRPSYFPFTEPSAEVDISCVICSGSGCRVCKSSG
                     WLEVMGCGMVHPQVFRHCGIDSELWSGFAFGMGVERLAMLRYGVDDLRLFFENDLRLL
                     EQFH"
     gene            complement(9946..10305)
                     /gene="rplT"
                     /locus_tag="PKI23_07240"
     CDS             complement(9946..10305)
                     /gene="rplT"
                     /locus_tag="PKI23_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNN36768.1"
                     /translation="MARVKRGVQAHRRHKKILDQAKGYYGARSRVYRVAKQAVIKAGQ
                     YAYRDRRQRKRAFRALWITRINAAARLHGLNYSRMIDGLNKSSIQIDRKILAELAMHD
                     QDAFAALASRAKAALGG"
     gene            complement(10345..10539)
                     /gene="rpmI"
                     /locus_tag="PKI23_07245"
     CDS             complement(10345..10539)
                     /gene="rpmI"
                     /locus_tag="PKI23_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012550352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNN36769.1"
                     /translation="MPKIKTNRGAAKRFRKTATGFKHKQSFKNHILTKKSTKRKRQLR
                     PMHAVDHADQSAVARMLPYL"
     gene            complement(10604..11146)
                     /gene="infC"
                     /locus_tag="PKI23_07250"
     CDS             complement(10604..11146)
                     /gene="infC"
                     /locus_tag="PKI23_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNN36770.1"
                     /translation="MTIKRESGRGSEKNRINGAITAPQVRVVDAEGQQVGVIPLSEAL
                     RLAEEAKLDLVEIAASADPPVCKIMDYGKYVFEAKKQKAAAKKKQKQIQIKEVKFRPG
                     TDEGDYQVKLRNLIRFLEEGDKAKVTLRFRGREMAHQRLGVELLKRIEQDLEPHGVVE
                     QMPAMEGRQMTMVISPKKKK"
     gene            complement(11143..13068)
                     /gene="thrS"
                     /locus_tag="PKI23_07255"
     CDS             complement(11143..13068)
                     /gene="thrS"
                     /locus_tag="PKI23_07255"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNN36771.1"
                     /translation="MPTITLPDHSRRQFDHPLTVQQVAAAIGPGLAGAALAGQVNGRL
                     VDTSFVIDQDAELAIITARDEAGVEVLRHSCAHLLAMAVKGLFPTAQVTIGPVIEEGF
                     YYDFAYERPFTPEDLEKIEARMVELAERDIPVVRQLMSRDQAIALFESMGEQYKVEII
                     REIPASEALSCYRQGDFIDLCRGPHVPSTGKLKAFKLTKVAGAYWRGDSRNAMLQRIY
                     GTAWPDTKALKQYLHRIEEASKRDHRRIGKQLDLFHAQEEAPGMVFWHPKGWTLYQVI
                     EQYMRRVQQAHGYQEIKTPQLVDYSLWHRSGHADKFGKEMFSTESESRNYAVKPMNCP
                     CHVQVFNQGLRSYRDLPLRLAEFGSCHRNEPSGTLHGLMRVRAFTQDDAHIFCSEASI
                     QQEVADFIDLLFKVYRDFGFDEVQLKLSTRPANRVGSDEVWDKAEAALAEALDQKGLN
                     WDLLPGEGAFYGPKIEFSLKDCLGRIWQCGTIQVDFSMPGRLDAQYVGEDGQRHVPVM
                     LHRAILGSFERFIGILIEHHAGAFPVWLAPVQLVVNSITDAQANYCREVTEQLVAAGF
                     RAVPDLRNEKIGFKIREHTLQKVPYLLVIGDREVESNQVAVRARDGKDLGQMSLAQLV
                     ALLNVQMAQLGRVSVEN"
     gene            13285..14244
                     /locus_tag="PKI23_07260"
     CDS             13285..14244
                     /locus_tag="PKI23_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNN36772.1"
                     /translation="MDSINLEVIGQLTHWLQSGQSGWLCTITATFGSSPRPVGSILAC
                     NRSGEVRGSLSGGCVEDDLIAALRSGEIASERPEELSYGVSKAETERLGLPCGGRLSL
                     VVEPLSPTAATQSHFAAIFTAIEQRRLIGRTLDLSSGQMQLFQPQHYSPTRLAQQRLT
                     QVFGPRYRLLLVGAGQIARYLAEMALALDFEVILTDPRPELLAAWNGPPITLAGGMPD
                     DAVRHWADGANSAVVTLTHDPRIDDMALMEALNSSAFYVGALGSLRTSAQRRERLEQL
                     DLPKSAIARLDAPIGLPIQSKTPPEIAISILAKLIQVRARVDG"
     gene            14231..14827
                     /locus_tag="PKI23_07265"
     CDS             14231..14827
                     /locus_tag="PKI23_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNN36773.1"
                     /translation="MSTAEPTPRHALLLLAAGAGRRYGADKLMAPLRQGGVLLEASLQ
                     PLTPWKQDVHVMLRPGASEAWRLVEQWGGHPHWSPDAALGMGHTLAAGIALLEGYTGC
                     LLLLGDMPGIRRQSIATLLARLDEHALVVPRHAGQWGHPVGFGRQYFAPLRQLTGDRG
                     ARGLLEQHQAQITFVDLDDPGILFDVDTAADLDRFQSP"
     gene            complement(14859..>15168)
                     /locus_tag="PKI23_07270"
     CDS             complement(14859..>15168)
                     /locus_tag="PKI23_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36774.1"
                     /translation="SCDIGSSHLHVLHHQTGTALTYAPIGSTTTVLNNSKPMSLSSLD
                     LGPGIASMPVIHQGAGDDGITVITQQSTGSLTGSDVNLQPALRARQSWLELDPAEVFK
                     "
BASE COUNT         3035 a         4897 c         4308 g         2928 t
ORIGIN      
        1 ccgccgctac ctcaaccgtg gcctctctct gctcgacgtg atcgaagagg gcaacctcgg
       61 gctgatccgg gccgtcgaaa aattcgaccc cgagcgcggc tttcgcttct cgacctatgc
      121 cacctggtgg attcgtcaga ccatcgagcg ggccatcatg agccagaccc gcaccatccg
      181 gctgccgatc cacatcgtca aggagctcaa cgtctacctg cgggcggccc gcgagctgac
      241 caagcacctc gaccatgatc ccaccgccga cgatgtcgcc aagctgctcg acaagccggt
      301 cgacgacgtg acacgcatgt tcaagctgaa cgagcgcatc acctcgatcg atgtgccggt
      361 tgccgccgat tccgaccgct cggtgctcga taccctcccc gatctgcacc tgccggatcc
      421 ggtcgaaacc ctgcaacaga acgacctgca cgccaacctc ggcaaatggc tcgaccaact
      481 gtcggacaaa caggccgaag tgctgtcacg ccgctttggt ctgcgcggtt atgaaatgac
      541 cacgctcgag gatgtcggca aggagatcgg cctcacccgc gaacgggtcc ggcagatcca
      601 ggtcgaagcc ctgcggcgac tgcgtgaaat catggaagaa aatggcctca gcagcgaagc
      661 ggtcttcagt tgaagccacg atccggttac cgcccgaccg gctcgccggt gggcggcggc
      721 tgacccgtcc ggcacgtcac cgcacttcat gctgaactgc caccgatgag catcgatcag
      781 ccacgttttt ccgcctgctt catgggcacc ggtccggctg gcggcagcga actcggcagt
      841 tcggcctgcg tcatcgaaga gtctggccaa ccccagctgc tgatcgactg cggccctgac
      901 accctgctgc gctatgaagc ctgctatcag cggctgcccg aagcactctt catcacccac
      961 tgccacttcg accacatcgg cggcctggag acgctcttct accgcgccac cttcgcacct
     1021 gaaccacggg agatccggct gttcgttccg gtgaccctgc tgaccaccct gcaccatcgg
     1081 ctggcagccg gcgtcagttg cctggccgaa gggggcgtca acttctggga ttcattcagg
     1141 ctgattccgg tcgataccgg cttctggttg aagcacaacc actatcgggt ctttcctgcc
     1201 cgccaccatc tgcccgacag cgcctttggc ctggctctgc cgggtcagtt cttctacagt
     1261 ggcgacaccc ggccgattcc ggagcaactg ctgctacatg ccagtcaggg agaagtgatc
     1321 ctgcatgatg cccgtgtcac cggcaatcca tctcacagcg gtctcgatga cctgctgcgc
     1381 gaatacgatg ccgcacagcg tggccgcatg gtgatttatc accatctgcc cggtgacgaa
     1441 ccactgtttg aaagcgctgg attcgccgtg gccagcccgt ttcgaaagat aacccttggc
     1501 gcaggccacg tccggcagac gtgaaacgaa gaagagatcg gaattggtcg ggacgacagg
     1561 atttgaacct gcgaccacca cacccccagt gtggtgcgct accaagctgc gctacgcccc
     1621 gatactcggc atcacgggcc caagggcctg caaaggcgcg aactatagcc gaaagcggcg
     1681 gttgacgaaa ggtttgcggc acttctgaac ccatggactc acccacccag gatgtcactt
     1741 gaacaaccgc cccgaccacc aactcgatcc accggttctg ttcatcgagc aacccaaccg
     1801 ccacggcggc aagatcggcc atgcgatcct gaatgccgag cgcaccctca atgcgctgtc
     1861 gctgccgatg atcgaactgc tcgaccagca gctgcggcga tgggccgagg atcctgaact
     1921 gattctggtg gtgctgaagg gtgccggcca gcgcgctttc tgtgccggcg gcgatgtggt
     1981 cgaactccat cgcagcatga tcgcccaccc cgacggaccc aatccctacg ccgaagcgtt
     2041 cttcagccgc gaataccggc tcgatcacct gctgcaccgc tatccaaaac cgctgctgtg
     2101 ctggggatcc ggcatcgtca tggggggtgg atggggattg atggccggtt gcagccaccg
     2161 ggtggtcacg cccgactcac gcctggccat gccggaaatc cgcatcggcc tctatcccga
     2221 cgccggcgcc agttggtttc tcgggcaact gcccaggcag atcagcctgt ttctcggttt
     2281 gaccggcgcc ccgctcaatg ccagcgatgc gcggctgctt ggtgtggccg atcacctgct
     2341 cagccgtgaa aaccaggtcg aggtcgaaca gcgcctgcaa cagacgcagt ggagccagga
     2401 gagcaccagc aaccaccagc acttgagtgc gctgctcgca gagatcgctg gaaagcaatc
     2461 tgcccagcca cctgaatcag caaccgaaaa acaccagagc ctcattcatg aaacctgcgc
     2521 ggccactgag ttggccgctg tcgtggaggc catcctggaa ctgccacagc atgatccatg
     2581 gcttgccggc gcggtgaaaa atctgcgaga agggtcgatg atttcggcgc tgctcacttg
     2641 gcaccagttg cgcgaacagc cgcctgcttc actggctggg gcgttccgac aggagttggt
     2701 gctgtcacta cagtgcgcct gtcagcacga attcaccgaa ggagtgcgcg cccggctggt
     2761 cgaacgtgat caacggccaa agtggcgcta tccttctatt cgcgcgctgc ctgaccgact
     2821 gctcgaaagt tttattgaag cgccgtggcc ggatggcgga catccgctgg ccgatctgaa
     2881 ctagaggtga cgagaacgaa gatgggggaa aaataaaagg ggcgcccatc cagtacgccc
     2941 cttttatcca gcctaacaac cctgtcggct cagttccgtt gcaacctccc tatttgtgca
     3001 tcctgcattg cgcctcctgc attgcttcca tgcgggagca aatctagcaa aaccctgtgg
     3061 ctttgaatca ccccgctcaa gcaaaaagcg cagacgacag cgctgaagaa tataaaataa
     3121 atattgttat caatctatta agaataattt gacgcattgg tcagcaaatt tttaagaaga
     3181 gaccgccgac gatttgagac atctcttaga aaaaagatgc cgatatcgca ggccgtgctc
     3241 ccacttcacg gtggcagatt gcctcctcac cgaccatcgg gcacactgcg agggccggat
     3301 tgaaacggac cacacccatc atcaagcttt tcaaggagtc ctcatggcca atctctttgc
     3361 ggacaacagc ctgtcgatcg gcaatacccc gatggtcaag ttgaatcgcg tgacccaagg
     3421 cagcgtgtac gccaagatcg aagggcggaa tcccgcctac tcggtcaagt gccggatcgg
     3481 cgcgtcgatg gtgtgggatg cggaaaaatc aggccgactg aagccgggca tggaactggt
     3541 cgaacccacc agcggcaaca ccggcatcgc actcgcctat gtcgctgcgg ccagaggcta
     3601 tggactgacc ctgacgatgc cctcaagctt ctctctggag cgtcgcaaga tcatgcgtgc
     3661 gcttggcgcc aagctgatat tgaccgaccc atccaagggg atgaacggtg ccatcgtcaa
     3721 ggcgcaggag atcgcccaga gcaaccccga ccgctacttg ctgttgcagc agttcgagaa
     3781 tccggccaac cctgccatcc atgaacagac cacaggtcca gaaatctggc gcgacaccga
     3841 tggcaaaatc gacatcctgg tcgcaggcgt tggcaccggc gggaccttga ccggcgtttc
     3901 acgctacatc aagcagaccc agggcaaacc gatcaccacc gtggccgtgg agccccttga
     3961 ctcctccgtg atcaaaaaca ccctcgaagg caaggcaccc gcccacaagc ctcacaaaat
     4021 tcagggcatc ggggcaggtt tcgtgccgaa aaatctcgat ctcggtctgg tcgatcaggt
     4081 cgaacaggtc agcaatgacg aggcgatcgc cttcgcccac cgcctgatgc gcgaagaagg
     4141 cattctggca ggcatctcct gtggcgccgc tgccgccgca gcagcccgcg tcgccgcaca
     4201 gccccagcat cagggcaaat gtatcgtggt gatcctgccc gactcggcgg agcgctatct
     4261 gtcgagcgca ctcttcgaag ggctgttcac cgaggccgaa ctgcagcaat gaagcacaag
     4321 ccattgcagg ggcggcgctg acaacaatca gccgctcact ctgccgtttt ggcctgtgcc
     4381 tgcagcagac cgtaaagctg atccttcagc cacaggcgtt cacgcttgag gccttcgatg
     4441 tagccatccg agctgacttc gatgccggtc tcgatgcgtc gcacctcccg gtcaacatcg
     4501 tggtagcgtt cgaacagcct ggcgaagtgc gcatcattca ttttcagacg atggatcgct
     4561 tcgcggtatt caggcaattc gtgaatcaaa tcatgcttgt ccatcttcac cccttctgct
     4621 gcgtcggctc atcttctcat accctcgagg cggcaccatg acctgcatca agcaacagcg
     4681 acaccccgat ggcgattgcg atcgtccgta ccctctcaga caagcgggca tctgccgcag
     4741 tcaagcgact ctcggcagag ggcaatcgaa tcaatgcaga gtgcgaggtt ccagatcgtc
     4801 atcggcaccc tgatcaagat gagcagagaa cgaatgggct ctgccgatga gctggattcc
     4861 agcctggatc atcgccttgg cgatttcgac atgggaggaa ttcagatagg agatggcctc
     4921 atcggagaag cgcagctgga tcagcggatc acccgcgcca tcactgcgct gcagcaccac
     4981 ttcaccatcg gcgagctcga caatttccag aaaggttgcg gccatctatc actcctgctt
     5041 cagggagcct tgaaaatacc ccccgaagcg caccgctgtc aacgcaaccg ccttgttacc
     5101 actgcgccat gccttgccgc tgccgctcta tcaactccat ggccgcactc aaccagctcg
     5161 cgcattcagc gacagtgggc agaacggccc gctgttcagc aagctcttgc agatcaagca
     5221 aggtcatgtg cgattccagg ccattccgtg ccggctcgct gttgacgcca atcccctcgc
     5281 tgcacgcatg gtaaaaaaca gcgaggcgct tcagccagga gtcgccacgc gatgcaagaa
     5341 ttcgcagctc actgatttcc ggtgaggctc cacaccgctc ggccagcagt gacagcatcg
     5401 attcaagatc cggcgcaggc agcggatcaa tcttgtaaaa acccgccact tcaatcagat
     5461 ggcagcgcac cgcaccagtc agatgaaaag caaaggattc gctgtaggca acccgccgcg
     5521 acagcaggtc ggtggcggag agtcccgcca tcttttcgag ttcacgcaga tggatgcgtg
     5581 caaaatggag cttctggcca acccgcctgg gcaacgcatc gttcatgcat ggacctcagc
     5641 ttcgattgcc ccgcacggtg cgcacacccg attcaatgct cgtttttcat ggatgcgaaa
     5701 ctgggtgcag ccggcatggc tggtgcgatg aaacaacatt cggcaaaagc gcgaccggcg
     5761 gccaatcact ctttcaactg gagcagaagc tgttccagtt ccgcgatcat ctgacgcacc
     5821 aactgccggt attgtgtctc ttcatgcttg gcttcatcac ctgacagccg ctgacgtgcg
     5881 ccgccgatgg tatagccctg atcgtagagc aggctgcgaa tctgcctgat cagcagcacc
     5941 tcctgccgct ggtagtaacg gcggttgccg cgccgcttga ctggattgag ctgcgggaat
     6001 tcctgctccc agtaacgcag cacatggggt ttgacgtcac acagttcact cacttcaccg
     6061 atggtgaagt acttttttcc gggaatgacc ggcaactcat tgttttggct tggttccaac
     6121 atagttttcg acacgcgcct tcagtttttg ccccggacga aaggtgacca cccggcgtgc
     6181 agtgatcgga atctcttcac cggtcttcgg atttcgtcca ggtcgccgat tcttgtctcg
     6241 caactcgaag ttgccaaatc cagacaactt gacgtgctcg tttgactcaa gacagagttt
     6301 gatctcttcg aagaagagtt caacgatctc cttcgcttca cgtttgttga gccccacctc
     6361 ttcaaacagg cgttcagcca tctccgcctt ggtgagcaca cgcgactcca tctgttgtta
     6421 tctcctcatc tccgcctgaa accgctgctc caatgcacgg atcaccgcat ccagactgct
     6481 ctggatttcc gcttcgccaa gagtgtgcga ttgtgattga aatatcaacc ccaatgccat
     6541 actttttctt ttaggatcaa tgccttttcc ttgatacaga tcaaacagga tcagctgcga
     6601 cagatcaggc ccggcagcgg ccttgatcgc ctgctcgagc atcctgaacg gcaactcccg
     6661 gtcgaccaga aacgccagat cccgcctcac gcttgggtaa tgagaccagc tcctgtaggc
     6721 ttcgagatga ttctcaagcg ctaaattcaa atttaattca aaaagatatg gaactgactt
     6781 caagtcaaag ctatctacaa gggtgggatg gagctgcccc atctgaccca gtaccttgcc
     6841 atcccggaca agctgtgccg attgtccggg atgcagtgcc ggatggtgtg ctggctgcag
     6901 ttcgagctga tgccgtgcgc cagtcagtgc aaaaagagtc tcgatgtcac ccttgagatc
     6961 aaagaaatcg accatttcac tgttggtgat gccccaggac tccggatggc gcgatcccgc
     7021 aatcactcca gccagcatcg gttcctgcag cagttgatca ccttgctgta caaagcgtag
     7081 cccactttca aacaggcgga cgcgggactg ctgccgattg aggttgcccc gcagcgccaa
     7141 tagcagaccg ggccagagac tgccgcgcat gaccgccatc tctttggaca tgggattggc
     7201 cagcggcaag gcttcgtggg ccggatcgat caggcggttc agctccggat cgacaaagct
     7261 gtaggtgatg gcctcttgat aaccccgagc ggtcaggcgc cggcgaaact cccgcagcga
     7321 cagggtccgt tcggagccgg caccaggctg gagcggcatg agtggaacac gggtgggcag
     7381 ccggtcatag ccatgcaacc gtgccagctc ttcgatcaga tcgacctcga tgttcagatc
     7441 gaaacgaaaa ctgggcactg tcacgcacca ctgcccggca gcctcggact cgatccggca
     7501 acccaggcgg ccgagcagat cttccacctc cgtcggcgcc atctccatgc ccagcagccg
     7561 gttgatcctc gatgcccgca acgcgatgac tgcgggcttt ggcagcagtt cgggctgcac
     7621 ctgctcgatg atcggaccgg gcaccccgcc cacgatcccg gtcagcaatg cggtggcccg
     7681 ctccagcgca cggcgcggca gttcaaaatc gacaccgcgt tcatagcgat gagaggcatc
     7741 ggtgtgcagc ccatgacgcc gggctcggcc ggcaatcgcc aatggggcga agaaggcgct
     7801 ttcgatgaac aggtggcgtg tcgtcgcggt gacgccagag tccgcgcccc ccatgatgcc
     7861 ggccatcgcc aacggcttga ccgcatcggc gatcagcaga acctcctgat ccaactgcaa
     7921 ctgctgacca tcgagcaggg tgatcgattc gcccgcgcgc gccatgcgca cctggatgcc
     7981 gccctgaatt ttctccagat caaaggcatg cagcggttgc cccaactcca gcatcacgta
     8041 gttggtcaca tccaccactg gactgatcga gcggacgcca ctgcgtcgca gccgttcacg
     8101 cagccacagc ggtgttttgc agtccggctg gatgttttct atcacgcggc cgagataacg
     8161 cggacaggcc tcgggtgctt cgacccgcac gggaatgacc cgctcgctca acgcgggtac
     8221 ggtgcgctcg gccggagcac aaaccgcctg acgcgccagc acccccacct ccctggccaa
     8281 tccggcaatg ccgagacagt caccgcggtt cggcgtcaga ccgatctcga tgatctgatc
     8341 atccagttgc agcacatcac tcagcgcggc accaagcggt gcctgcgcat ccagttccag
     8401 caaaccctcg cctgccagat cgaggcccag ctcactggct gcggccagca tgcccgccga
     8461 atggactcca cgcacagtgg tggcgacgat ttcgctgcca tccggcaggc gagcgccagc
     8521 cagcaccagg ggcacgcgca ggccgactcg cacattgggg gcgccacaga caacttccaa
     8581 ctccccccgc tcacccgcgt tgacgcggca gagtctgagc cgatcggcat tggggtgcgg
     8641 ctgcaccgcg atgatctcgg ccaccaccac accactgagt gcgggcgctg ccgcactctg
     8701 gccatccact tcgaggccag ccatcgtcag gcgggccgac agctccgccg tggcaaaagg
     8761 cggatcgatc cattcacgca accaggcttc actaaacttc atgcaggtcg tccagattcg
     8821 gtttgatcag gcgcagacat ggcaagattc ggctcaatgg aactgttcca gcagccgcag
     8881 atcgttttca aagaagagtc gcagatcatc gacaccataa cggagcatcg ccaaccgctc
     8941 cacgcccatg ccgaaggcaa agccgctcca cagctcgctg tcgatgccgc agtgccgaaa
     9001 cacctgcgga tggaccatgc cacaccccat cacctcgagc cagccgctcg atttgcagac
     9061 ccggcagcca cttcccgaac agatcacaca cgaaatatcg acctcggccg agggctccgt
     9121 gaaggggaaa taggagggtc tgaatcgcac ttgcagatcg cgctcgaaaa aactgcgcag
     9181 aaaaccgctg acggttcctt tcagatcagc gaaggagatg tcgcggtcga ccaccagccc
     9241 ctcgacctgg tggaacatcg gcgtatgagt cacgtcagag tcacaacggt agacgcgccc
     9301 cggacaaatc aggcgaaacg gtggctggcc ctgcttcatc tgccgaatct gcaccggtga
     9361 ggtgtgggtt ctcagcaacc ggtgcgcatc aatgtagaag gtgtcatgca tggcccgcgc
     9421 cggatggtgg gccggaatgt tgagtgcctc gaagttgtgg tagtcatcct ccacctccgg
     9481 cccttcggcc acttcgtagc cgatcgaggt gaaaatatct tccatccgct ccagcacctt
     9541 ggtgatcgga tgcagtgcac cactcgactg tccgcgcccg ggcagcgtca catcgaccca
     9601 gccttgcgcc agttgctgat cgagcgcctg cgcatccagt gtctgcttgc gctgcgccag
     9661 caacgcctgt aactgctcct tgacccggtt gacgagcgct cccgctgctc tgcgctcttc
     9721 ggccggcagt gctcccaact ccttgagctg ctgggtgatg acgccttttt tgcccagata
     9781 gaacacccga acctgatcca gagccatcag atcggccgag tcggcaatcg ccgcctcggc
     9841 atcgtgcaac agcttctcaa gactttccat catgcttcct tcaggataaa aaaaggggaa
     9901 gaccagcgct tccccttaac gtcaaaccct tgaagagcgt gctcttcagc ctcccagcgc
     9961 ggccttggcc cggcttgcca gagcggcaaa ggcatcctgg tcatgcatgg ccagctccgc
    10021 cagaatcttg cgatcgatct gaatcgagct cttgttgagc ccatcgatca tccggctgta
    10081 gttcagccca tgcaggcgtg cggccgcatt gatgcgggtg atccacagcg cgcggaaggc
    10141 gcgcttgcgc tgccgacggt cacggtaggc atactgtcca gccttgatga ccgcctgttt
    10201 ggcgacacga tagacccggc tgcgggcgcc atagtagccc ttggcctggt cgagaatctt
    10261 cttgtgacgg cgatgtgcct gcacaccgcg tttaacacga gccattgatg aatcctctgc
    10321 aaatcaacgt ggtctgaccg acactcacag atagggcagc atacgcgcca ccgccgactg
    10381 atcggcatgg tcgacggcat gcatcgggcg cagctgacgc ttacgcttgg tgctcttctt
    10441 ggtcagaatg tgattcttga aggattgctt gtgcttgaaa ccggtcgcgg tcttgcgaaa
    10501 tcgcttggcc gccccacggt tggttttgat cttcggcatt ttttcaccac tgcgcattcg
    10561 ttggatgatg accccaaggt tgcggaaaac cgcgacatcg gagctacttt ttctttttcg
    10621 gggagatcac catggtcatc tggcgtcctt ccattgccgg catctgctcc accacgccat
    10681 gcggttcgag atcctgctca atacgcttga gcagttcgac gccaaggcgc tgatgggcca
    10741 tctcccgacc ccgaaatctg agcgtgacct tggccttgtc accctcttca aggaaacgta
    10801 tcaggttgcg tagtttgacc tgataatccc cctcgtccgt ccctggcctg aacttgacct
    10861 ctttgatctg aatctgtttc tgctttttct tggcagcggc tttctgtttt ttggcctcaa
    10921 aaacatactt gccgtagtcc atgatcttgc agaccggagg atcggccgat gcggcgatct
    10981 cgaccagatc gagcttggcc tcttcggcca gccgcagtgc ctcactcagc gggatgactc
    11041 ccacctgctg accctcggca tccaccactc gaacctgggg agcggtaatc gctccattga
    11101 tccggttttt ttcggatcct cttcctgact cgcgcttgat tgtcaattct ccacggaaac
    11161 gcggccgagc tgcgccattt gaacattcaa aagggcaacg agctgtgcca gactcatctg
    11221 acccagatcc ttgccatcgc gcgcacgtac agccacttga ttggattcga cctccctatc
    11281 gccgatgacc agcagatagg gcaccttttg caaggtatgc tcgcggattt taaagccgat
    11341 cttctcgttt ctcaagtctg gaacggctct aaaaccggca gcgaccaact gttctgtcac
    11401 ttcgcggcaa taattcgcct gtgcatcggt gatgctgttg accaccagct gcaccggcgc
    11461 cagccagacc gggaaggcac cggcatggtg ctcgatcagg atgccgatga agcgctcgaa
    11521 ggagccgagg atggcgcggt gcagcatgac cggcacatgc cgctgcccat cctcacccac
    11581 atactgcgca tcgagccggc ctggcatcga gaagtcgacc tggatcgtac cgcactgcca
    11641 gatgcgtccc aggcaatcct tcagcgagaa ttcgatcttc ggaccataga aggctccctc
    11701 gccaggcagc agatcccagt tgagcccctt ctgatcgagc gcctcggcca aggccgcttc
    11761 ggctttgtcc cacacctcgt cggagccgac ccgattggcc gggcgggtgg agagcttcaa
    11821 ctgtacctcg tcaaagccaa aatcgcggta gaccttgaac agcaggtcga tgaagtcggc
    11881 cacctcctgc tgaatcgacg cctcgctgca gaagatgtgg gcatcgtcct gggtgaaggc
    11941 gcgcacccgc atcaatccat gcagcgttcc cgaaggctca ttgcggtgac aggagccaaa
    12001 ctcggccagc cgcagcggca ggtcgcggta gctgcgcaac ccctggttga agacctgcac
    12061 atggcagggg cagttcatcg gcttgactgc gtagttgcgg ctctcggact cggtgctgaa
    12121 catctccttg ccgaacttgt cggcatggcc ggagcggtgc cagagcgagt agtcgaccag
    12181 ttgcggggtc ttgatctcct ggtagccatg cgcctgctgc accctgcgca tgtactgctc
    12241 gatcacctga tagagtgtcc agcccttggg atgccagaac accatgccgg gcgcctcttc
    12301 ctgcgcatga aacagatcga gctgcttgcc gatgcggcgg tggtcacgct tgctcgcctc
    12361 ttcgatacga tgcaggtact gcttgagcgc cttggtatcg ggccaggcgg tgccatagat
    12421 gcgctgcaac atcgcgttgc gggagtcgcc tcgccagtag gcacccgcca ccttggtgag
    12481 cttgaacgcc ttgagcttgc cggtgctggg cacatggggc ccgcggcaga ggtcgatgaa
    12541 gtcgccctgc cgatagcagg agagcgcctc gctggccgga atctcccgaa tgatctcgac
    12601 cttgtactgt tcgcccatcg actcgaacag cgcgatcgcc tgatcacggc tcatgagctg
    12661 tcgcactacc gggatgtcgc gctcggccag ctcgaccatg cgtgcttcga tcttttcgag
    12721 atcttccggg gtaaagggcc gctcataggc gaagtcatag tagaagccct cctcgatcac
    12781 cggaccgatg gtgacctggg ccgtgggaaa gagtcctttg accgccatcg ccagcaggtg
    12841 ggcacaggag tggcgcagca cctccactcc cgcttcatcg cgcgcggtga tgatggccag
    12901 ctcggcatcc tgatcgatga caaaggaggt gtcgaccagt cggccattca cctggccggc
    12961 cagtgcggca ccagcgagtc cagggccgat cgccgcagcc acctgctgca cggtgagcgg
    13021 atgatcgaac tgacggcggg agtgatcggg caaagtgatg gtgggcattt tgaatcctct
    13081 tgcagtggtg accgctacca aaggccacgt gtcgagcggt gatcgccggc agtgcaatca
    13141 cgatggcgca ggctggcatt atagccactc cactgattgg aatagaattc gatccggccg
    13201 tctcccgggt ccgctgccgg acaccgcagc aagacagccg cccgatgttt ttgatcaacc
    13261 aaccgtacag acaggaacca ccccgtggac agcatcaacc tcgaagtgat cggccagctc
    13321 acgcactggc tgcagagcgg tcaatcgggc tggctctgca ccatcaccgc caccttcggt
    13381 tcttcgccgc gtccggttgg ctccattctg gcctgcaacc gcagcggcga ggtgcgcggt
    13441 tcgctgtcgg gtggctgtgt cgaggatgat ctgattgctg cgctgcgcag tggcgagatc
    13501 gccagcgagc gccccgaaga gctcagctac ggcgtcagca aggccgagac cgaacggctt
    13561 ggtctgcctt gcggcggccg gctgtcgctg gtggtggagc cgctgtcacc gaccgcagcg
    13621 acccagtccc atttcgccgc catctttacc gcgatcgaac agcgccgcct gatcggccgc
    13681 acgctcgacc tctccagcgg tcagatgcaa ctgttccagc cgcagcacta cagccccacc
    13741 cgtctggcgc agcagcggct gacccaggtg ttcggaccgc gttaccgcct gctgctggtc
    13801 ggcgccggcc agatcgcccg ctatctggcc gagatggcgc tggcgctcga tttcgaggtg
    13861 atcctgaccg acccgcgtcc cgaacttctg gccgcctgga acggcccgcc gatcacgctg
    13921 gccggcggca tgcccgacga tgccgtgcgt cactgggcgg acggtgccaa cagcgcggtg
    13981 gtcaccctga cccatgaccc ccgcatcgac gacatggcgc tgatggaggc gctcaactca
    14041 tcggccttct atgtcggtgc gctgggttcg ctgcgcacct cggcgcagcg gcgcgaacgg
    14101 ctggagcagc tcgatctgcc caaatcggcg atcgcccggc tcgatgcacc aatcggcctg
    14161 ccgatccaga gcaagacgcc accggaaatc gccatctcca ttctggccaa gctgattcag
    14221 gtgcgcgccc gtgtcgacgg ctgagccgac gccgcgccac gcactgctgc tgctggccgc
    14281 aggcgccggg cggcgctacg gtgccgacaa gctgatggcg cccctgaggc agggtggcgt
    14341 gctgctggag gccagcctgc agccgctcac cccctggaaa caggatgtgc atgtgatgct
    14401 gcgccccggg gccagcgaag cctggcgcct ggtcgagcag tggggtggcc atcctcactg
    14461 gagccctgat gcggcgctcg gcatgggcca cacgctggcc gcgggcatcg cgctgctcga
    14521 agggtatacc ggctgcctgc tgctgctcgg cgacatgccc ggcatccgcc gtcagagcat
    14581 cgccacgctg ctcgcccggc tggacgagca tgccctggtc gtgccacgcc acgctggcca
    14641 gtggggccat ccggtcggtt ttggccgcca gtacttcgcg ccactgcgac agctcaccgg
    14701 cgatcgtggc gcacgcgggc tgctggagca gcaccaggcg cagatcacct ttgtcgacct
    14761 ggatgacccc ggcattctgt tcgacgtcga caccgccgcc gacctcgacc gcttccagtc
    14821 accttgaacc cgccgcgtgg cgggctcgat gcctcacctc acttgaacac ctcggccgga
    14881 tcgagctcga gccaggattg ccgtgcccgc agcgctggct gaagattgac atcggaaccg
    14941 gtcaggctgc cggtcgactg ctgggtgatc acggtgatgc catcatcccc ggctccctga
    15001 tggatcaccg gcatcgacgc gatgccaggc cccagatcga gcgagctcaa cgacatcggc
    15061 ttgctgttgt tgagcaccgt ggtggtgctg ccgatcggtg cataggtgag cgccgtgccg
    15121 gtctggtgat gcagcacatg gagatggctg ctgccgatgt cacaggag
//
LOCUS       DAORCQ010000056        15147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2506_length_15147_cov_5.897628, whole genome shotgun sequence.
ACCESSION   DAORCQ010000056 DAORCQ010000000
VERSION     DAORCQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 15147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15147
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_15147_cov_5.897628"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PKI23_07275"
     CDS             <1..1356
                     /locus_tag="PKI23_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNN36775.1"
                     /translation="VTNGYGTGSLQLKNNDTGQAVSLASSSGQSAAAIAAGINALNHS
                     GVSASAQTSVTLSAITGVATGEFSLNGTALTGNTVAAWVSQINAMSGFSAVDNGGGSM
                     TITQNSGQDIDFSITGTGSVALRGPGDSAASTLNAGNGFATVGGTVALTTSENYSYVT
                     GSGDGAVFPGTPSTGPVIASNTFDPSQQDSYSHATSATIYDSLGNAHTLSQYFVKESV
                     YKSGLDNAWTLYVKIDGRDVGDPLPNSSLPTQASYRLVFNADGSLDSANSDSILISNW
                     SPKDANGADNGALQGSPGTTLPLPVPSTSSAFVVEMDSSTQYGGPFAVNALSQDGYAS
                     GRLSGIEIATGGQIFSRFTNGQSKILGQVALANFSNPQGLSPTGGTRWVETYDSGAPV
                     VNTPGSSSLGVLQSGALEESNADLSNELVRLIVAQRNYQANAKTIQTYDAVTQSLLNL
                     R"
     gene            1654..2391
                     /gene="flgF"
                     /locus_tag="PKI23_07280"
     CDS             1654..2391
                     /gene="flgF"
                     /locus_tag="PKI23_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNN36776.1"
                     /translation="MDRGVYVAMSGAAQTMRAQTIHANNLANINTTGFRSDLAQARSM
                     PVFGEHHPSRVYAMTERPGSDLSSGTLTQTGNPLDIAVAGEGWIAVSGPDGREAYTRA
                     GDLRIDALGQLTTASGLPVLGNGGAIAVPPAQKIQIGADGTLSIVPQGGDAQTLTEVD
                     RIKLVNPDARQLRKEQDGLFRLAAGAVAEADATVRVESGAIERSNVNAVESMSEIITL
                     ARQFEMQVKMMKTFEENAAQSTRILQM"
     gene            2469..3254
                     /gene="flgG"
                     /locus_tag="PKI23_07285"
     CDS             2469..3254
                     /gene="flgG"
                     /locus_tag="PKI23_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNN36777.1"
                     /translation="MNGALWVSKTGLSAQDTALRVISNNLANVSTMGFKRDRAMFEDM
                     LYQIDKQPGGLSTQEYQLPSGLQLGTGVRTAGTQKVFSQGTLQVTEQPLDMAINGQGF
                     FQITMPDGSISYSRDGQFSLNSEGQIVTTNGLPLEPAITIPENTKNITIGQDGVVSVL
                     TAGATSPTEVGTIQLANFVNPAGLEAIGDNLFRETTASGTPQVGTAGLDGLGSLKQGS
                     LENSNVSVVEEMVNMITTQRAYEMNSKVISTADQMLQYLGQTL"
     gene            3288..3998
                     /gene="flgH"
                     /locus_tag="PKI23_07290"
     CDS             3288..3998
                     /gene="flgH"
                     /locus_tag="PKI23_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNN36778.1"
                     /translation="MTTVATGRKRARNSRRMSLLLLSLLSLVGCDVLPVKPDDPAYAP
                     VSPDRLMPTSQVNGAIYQDGQMVQLFGDRRAQRVGDVVTILLTENTASKKSAKTSTQK
                     DESATIANPSLFGHDVSIQGNDLSASLDAKRKFDGEGSSDQSNSLSGSITVTVVNVLP
                     NGLLEVRGEKWISLNRGDEYVRIRGLLRSEDISTSNTIPSTKLANARISYGGTGEVAD
                     ASAMGWLARFFNSPIWPF"
     gene            4012..5112
                     /locus_tag="PKI23_07295"
     CDS             4012..5112
                     /locus_tag="PKI23_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNN36779.1"
                     /translation="MRSIRPGLLCGLLLMALSSVTLADRLKDLASVSGVRTNQLVGYG
                     LVVGLNGTGDKAPFTDQTFSNMMAKFGIALPPGIDPKLKNVAAVAIHAQLPAFAKPGQ
                     SIDVTVSSIGNAKSLRGGSLLLAPLRGTDGQIYAVAQGNLVVAGFDASGNDGSRITVN
                     VPSTGRIPSGATVERMVQSSFGQGESVVFNLNRADFTTARRVTEAINSFMGPRAATAI
                     DASSIRVMAPIDMAQRVEFLSMLENIEVTPGDAAARVVINSRTGTIIIGQHVQVAPAA
                     VTHGSLTVTISENPRVSQPGAFSPGQTVVVPDSKIEVSEGNGHMFKFDAGVSLDTIVR
                     AVNGVGAAPSDLMAILEALKEAGALRAELVVI"
     gene            5128..6132
                     /gene="flgJ"
                     /locus_tag="PKI23_07300"
     CDS             5128..6132
                     /gene="flgJ"
                     /locus_tag="PKI23_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNN36780.1"
                     /translation="MKVDQSPLYNDLASVQQLGRGGDPQDPQRLAAAARQFESMFVQM
                     MTKAMRDANRALIGDSPFSSHESEIYQEMLDNQLSVMNGSGIGLAEVVLRQLQGGETG
                     APEALASMGYAAPARNPHLDLRSAQLAMRHRDPAPLEGPSAPPFKVPRLGVQSLLWDQ
                     DATGFSSSEAFSQQLYPAARQAASMLGVDPRLLLSQAALETGWGQRMISGNRGENSFN
                     LFGIKADASWQGPKVWVDTLEYRDGVAVRERAAFRAYGSYEESFADYARFIREQPRYQ
                     EALRQAGTPRGYIQALQQAGYATDPDYARKVMAIYRGDSIQQAPLAMNQSAAGTTSYQ
                     "
     gene            6145..8115
                     /gene="flgK"
                     /locus_tag="PKI23_07305"
     CDS             6145..8115
                     /gene="flgK"
                     /locus_tag="PKI23_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNN36781.1"
                     /translation="MAGDLLNISLSGIQANRLNLSVTGQNISNANVEGYSRQEANLST
                     RPAQRDGSFFVGSGVTIDQVRRVADQFLTRQIMLDSSQKQQTQAMQSGLDALDKMLSS
                     ENSGLTTDLNRFFLSFQAVAQSPSSVTAREVLLGDARGLANRFQTLGGDLGQRIKDVN
                     QQLDTLSGQINELSSAIARLNINIAVLSGNGSGAQPNDMLDQRDQLLRKLSELVQINT
                     ISQSDGSINVMLDKGSALVVGGQASSVSSGPDPNDNARSLLYLQLGTQRQSLSSGSLG
                     GQLGGLLDYRDQALAPTLTELGRIGGSIALLVNQQHQLGVDLEGRMGGLFFADPNSAT
                     LVAQRVQPNLNNPPPKDQVMAVQLLDPAQFKSSDYKLDLIGPANDQYTLTRLSDNVQI
                     SSGNFGALPASISVDGVQIDLTSGTFRAGDHYLITPFRAVPNSMAMAVSRPQSLALAQ
                     PVRGSATLTNQGSGALTTVEMTDRGPYDASTLALPLRITFTSGTAYTVTDGNSATVAA
                     GLPYPPTGASGMIPAALGLKVTMSGSPVAGDSFTVQFNSQAVGDNRNALALGALGNRP
                     LLEQGTLALIESDRVMTGRVGTLAARAKVAFDSADASLRQSQAQRDAMSGVNLDEEAA
                     KMIEFQNSYNASARLIQVASELFDTLLNSVGR"
     gene            8134..9378
                     /gene="flgL"
                     /locus_tag="PKI23_07310"
     CDS             8134..9378
                     /gene="flgL"
                     /locus_tag="PKI23_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNN36782.1"
                     /translation="MRISTMQFYAQARNAILDNQSKLLETHDQLATGKRVTQAGDDVV
                     AFVAIQRLRAEEQSTQRYLDNITAARQRISSEDTTLDQITNSLQRVRELAIQSESGAL
                     NTADRAGIASELRALRDQLLGQLNSRDGDGHYLFSGYQANTAPFASDASGAFSFQGDE
                     GIAELTVAPGVTMPLGHDGRWLFVDLLAPRNVTATAAASNTGTVTTAQPIVTDQAQVD
                     GWAGKSLSIDFTSASQFVITEQPANIPVAIQKGVPAGYLPGTPIDYVAGMELEFAGTQ
                     IKLSGAPQSGDRLTLTVAAAGRQDLLTSVDALARSITDASLNTAQRSEAIADAIQQMD
                     ASRDRISQARAQVGARLNSLDALESNHQQSTLQTKEVLTNLEDLDFTEAISRLSQQTA
                     ALQAAQQSYATLSRLSLFNLLS"
     assembly_gap    9523..9622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9966..12512
                     /gene="mutS"
                     /locus_tag="PKI23_07315"
     CDS             9966..12512
                     /gene="mutS"
                     /locus_tag="PKI23_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNN36783.1"
                     /translation="MMQQYLRLKVEHPDALLFYRMGDFYELFYDDARQAAQLLGITLT
                     SRGQSAGEPIPMAGVPHHAAEGYLAKLTRLGLSVAICEQIGDPASSKGPVERKVVRVV
                     TPGTISDEALLESDRENLLVAISGDGQRFGIASLELSSGRFRLCEPESPTALIEELGR
                     LQPAELLIDESLSLTLPKPYQPLLRQRPNWEFDHDSAQAALTRQFRTRSLAGFGCEGL
                     RLAIGAAGALLHYARATQRSELPHLERLEVEHRDDSVLLDSATRRNLEIDRNLAGGRS
                     NTLLSVLDRTATPMGSRLLSRWLHRPLRDQQQLGHRHLAIEQLIDGRRHEEIHALLRR
                     VDDLERILGRVALRSARPRDLTRLGQALRLLPDLHRLLHGVDAPRLLALDAAVGLFPE
                     LVELLEAALLPEPPVLLRDGGVIAPGFSAELDQLRAMGQDAGAFLMQIELRERERTGL
                     SSLKVRYNRVHGYYIEISRSQADLAPPEYQRRQSVKHAERFMIPELKQFEEQALSSQS
                     RALALEKMLYEGLLDSLNLSLGPLRASAEALAELDLLANLAERAHTLGYQRPQFCAQP
                     RIEIRQGRHPVVEQVSSDPFIANDLELDDQRRMLIITGPNMGGKSTYMRQNALIALLA
                     YSGSFVPARSAILGPIDRIFTRIGSADDLAGGRSTFMVEMSETANILHHATANSLVLM
                     DEIGRGTSTFDGLALAWASARHLAERAGALTLFATHYFELTALAEQLPAVANVHLAAT
                     EYGERIVFLHGVREGAASQSYGIHVARLAGLPGAVITDAEARLMQLEALSRGAQVASA
                     EPPLQGDLFAPVDLAHSVLKRLQAVDPDRMTPRQALEWLYQLKALTETSR"
     gene            12622..12945
                     /locus_tag="PKI23_07320"
     CDS             12622..12945
                     /locus_tag="PKI23_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNN36784.1"
                     /translation="MTFVVTENCIKCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCG
                     LCEPECPVEAIFDEDELPAGQEEFVEINARLAESWPNITHKKDAPADADDWKGVTGKR
                     KYLEE"
     gene            13047..13865
                     /locus_tag="PKI23_07325"
     CDS             13047..13865
                     /locus_tag="PKI23_07325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNN36785.1"
                     /translation="MHPMLTIAQRAARRAGEKIAYSIDRLSQIKVEEKGRHDYVSEID
                     KAAEALIIETLQRSYPEHGILGEESGLIEGRGAGRDYLWVIDPLDGTTNFLHGVPHCA
                     VSIGLRHRGQLDQAIVLDPFRQEEFTASRGAGAQLNGRRIRVSDRKSFPGSLLATGFP
                     FKPVTAQTQGKVSHYFRTLEQLAGVTSGIRRAGSAALDLAYVAAGRYDGYWELDLQAW
                     DLAAGALLVQEAGGLVSDHAGGQNFLDGGDIVAAAPRCHKVLLDMIRHGGGEKS"
     gene            13900..14589
                     /gene="nth"
                     /locus_tag="PKI23_07330"
     CDS             13900..14589
                     /gene="nth"
                     /locus_tag="PKI23_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNN36786.1"
                     /translation="MLSAAECHELFRRLQAANPKPTTELHYRNPFELLIAVMLSAQAT
                     DVSVNRVTPALFAAAPTAQEMARLGSEAIKGHIRTLGLFNSKAEHISATCRLLVERHG
                     GEVPDSRAALEALPGVGRKTANVVLNTAFGEPVIAVDTHLFRVANRTGLAPGKSVQQV
                     EQGLMKRVPKPFLHDAHHWLILHGRYLCTARKPHCDRCPLADLCRFEDKRLGPPSDRP
                     ETTQPNPEEHP"
     gene            14586..>15147
                     /locus_tag="PKI23_07335"
     CDS             14586..>15147
                     /locus_tag="PKI23_07335"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNN36787.1"
                     /translation="MSDAVFIREGEFFHPQPNAGSPWGPATLHGAAPAGLIGHALEQL
                     LPSPEMQLARMTIDLFRPVPMAPLRLSSEVVRAGRKIHVVQVSLWADAVEVCRASGVM
                     LRRTPIDLPANLLPAADKPAGPEGLAITGIRDAAAGESAFALLPGFHTAIEVKRVSGG
                     KGQGAGCAWLRIPIPLIDGLETTPLMR"
BASE COUNT         2742 a         4948 c         4877 g         2480 t
ORIGIN      
        1 gtgaccaacg gctatggcac gggatccttg cagttgaaga acaacgacac cggtcaggca
       61 gtgagtcttg ccagcagcag cgggcagagt gcagcggcca tcgcggccgg aatcaatgcg
      121 ctgaaccact ccggcgtctc ggcttcagcg cagaccagcg tcacgctgtc ggcgatcacc
      181 ggggtggcca ccggggagtt ctcactgaac gggaccgcgc tgaccggcaa cacggtcgcg
      241 gcctgggtca gtcagatcaa tgcgatgagt ggcttctcgg cggtcgacaa tggtggcggt
      301 tcgatgacca tcacccagaa cagtggtcag gacatcgatt tctcgatcac cggcaccggc
      361 tccgtcgcgc tgcgtggccc gggtgacagc gctgcatcga ccctgaatgc aggcaatggc
      421 tttgccacgg tcggtggcac ggtcgcgctg accaccagcg agaactacag ctatgtgacc
      481 ggcagcggtg acggggcggt ctttcctggt acgccctcga ccggtccggt gattgccagc
      541 aacaccttcg acccctccca gcaggacagc tacagccacg ccacctcggc caccatctat
      601 gacagcctgg gcaatgccca caccctgagt caatatttcg tcaaggagtc ggtctacaag
      661 agcggcctcg acaacgcctg gacgctctac gtcaagatcg atggtcgcga tgtcggtgat
      721 ccgctgccca actcgtcgct gccgacccag gcgagttatc ggctggtgtt caatgccgat
      781 ggcagcctcg acagcgccaa ctccgattcg atactgatct ccaactggag cccgaaggat
      841 gccaatggcg ccgacaatgg cgcactgcag ggctcacccg ggaccacact gccgctgccg
      901 gtaccctcga ccagttcggc cttcgtggtg gagatggatt cctccaccca gtatggcggt
      961 cccttcgcgg tcaatgcgct gagtcaggat ggctatgcat cgggccggct gagcggcatc
     1021 gagatcgcca ccggcgggca gatcttctcg cgcttcacca acggccagtc gaaaattctg
     1081 gggcaggtgg cgctggccaa cttctccaat ccgcaggggt tgagcccgac cggcggcacc
     1141 cgctgggtcg agacctatga ctccggcgcg ccggtcgtca acacgccggg ttcgtcgagt
     1201 cttggcgtgt tgcagtcggg tgcactggag gagtcgaatg ccgatctctc caacgaactg
     1261 gttcgactga tcgtggcgca gcgcaactat caggccaatg ccaagaccat tcagacctat
     1321 gacgccgtca cccagtcgct gctcaatttg cgatagcgac gcgcttgact gagtcgtgaa
     1381 ggggccgttg tcgttgcgca gtggccccga ttttgtcgcc atccccgctt catgcaccgg
     1441 cggcaagccg ttgccgccct cctctccatg ctctgccgcc ccggcggaaa aatctgcacg
     1501 tcggcgtgcc gctgcggcgc tgtctgcaat cttttttcat aacggattgt ttttgattgg
     1561 ctttttatga aggggctctg tcaataaacc gacggattga gttggcccgc gatctgcatt
     1621 gacagatcaa cagcgattca accggagcag acaatggacc gcggagtgta tgtcgccatg
     1681 agtggtgccg cgcagaccat gcgggcgcag accatccatg ccaacaacct ggccaacatc
     1741 aacaccactg gctttcgcag cgatctggcg caggcgcgca gcatgccggt gtttggcgag
     1801 caccatccga gccgggtcta cgcgatgacc gaacggcccg gatcggacct ctcgtcgggc
     1861 acgctgacgc agaccggcaa cccgctcgac atcgccgtgg cgggtgaggg ctggatcgcg
     1921 gtgagtggcc cggatgggcg cgaagcctac acccgcgccg gcgatctgcg catcgatgca
     1981 ctggggcagt tgaccaccgc cagcggtctg ccggtgctcg gcaacggcgg cgccatcgcg
     2041 gtgccgccgg cgcagaagat acagatcggc gccgatggca ccctctcgat cgttccccag
     2101 ggtggcgatg cgcagaccct gaccgaggtg gaccggatca agctggtcaa tccggacgcc
     2161 cggcagttgc gcaaggagca ggatggtctg ttccggctgg ccgctggcgc agtcgccgag
     2221 gccgatgcca cggtgcgggt cgagtcgggc gccatcgagc gcagcaatgt caacgcggtc
     2281 gagtcgatga gcgaaatcat cacgctggcg cggcagttcg agatgcaggt gaagatgatg
     2341 aagacctttg aagagaacgc cgcgcagtcg acgcggattc tgcagatgta acggggcgtc
     2401 acggcgccag aaaactgccg cgtcacggtg gcgcgattcg atcaacacgg caagcaggaa
     2461 aagacgacat gaatggtgcg ctttgggtca gcaaaaccgg attgagtgcg caggacaccg
     2521 cgctgcgggt catctccaac aacctcgcca atgtctcgac catgggcttc aagcgcgatc
     2581 gggcgatgtt cgaggacatg ctctatcaga tcgacaagca gcccggcggg ctctcgaccc
     2641 aggagtacca gttgccctcg ggcctgcaac tcggcaccgg ggtgcgcacg gccggcacac
     2701 agaaagtttt cagccagggc acgctgcagg tgaccgagca gccgctcgac atggcgatca
     2761 acggtcaggg cttctttcag atcaccatgc ccgacggcag catcagctac agccgcgacg
     2821 ggcagttcag cctcaactcc gaaggtcaga tcgtcaccac caacgggctg ccgctggagc
     2881 cggccatcac cattccggag aacaccaaga acatcaccat cggtcaggat ggcgtggtca
     2941 gcgtgctgac cgccggtgcc acctcgccga ccgaagtggg caccattcag ctggccaact
     3001 tcgtcaatcc ggccggactc gaagcgatcg gcgacaacct gtttcgtgaa accaccgcca
     3061 gcggcacgcc gcaggtgggc accgccggac tggatgggct gggcagtctg aagcagggct
     3121 cgctggagaa ttccaacgtc agtgtggtcg aagagatggt caacatgatc accacccagc
     3181 gcgcctacga gatgaattcg aaggtgatct ccaccgccga ccagatgctg caatacctgg
     3241 gtcagaccct gtaaccggtg aagcacgatg gtgagaggta tccgctgatg accactgtcg
     3301 cgaccggccg caagcgagcg cgaaacagcc gccggatgag cctgctgctg ctgtcgctgc
     3361 tgagtctggt cggctgtgac gtgctgccgg tcaagccgga tgatccggcc tacgcgccgg
     3421 tctcgcccga tcgcctgatg ccgacctcac aggtgaacgg cgccatctat caggatggcc
     3481 agatggtgca gctgttcggc gaccggcggg cgcagcgggt cggcgatgtg gtcaccatcc
     3541 tgctgaccga gaacaccgcc tcgaagaaga gcgccaagac cagcacccag aaggatgagt
     3601 cggccaccat cgccaatccg tcgctgtttg gccacgatgt cagcatccag ggcaacgacc
     3661 tgagcgcctc gctcgatgcg aagcgcaagt tcgatggcga gggcagctcc gaccagagca
     3721 acagcctgag cggcagcatc accgtcaccg tggtcaatgt gctgcccaat ggcctgctgg
     3781 aggtgcgcgg cgagaagtgg atctcgctca accggggcga tgagtatgtg cgcatccgcg
     3841 gcctgctgcg ttccgaggac atctccacca gcaacaccat cccctcgacc aagctggcca
     3901 acgcgcgcat ctcctacggc gggaccggcg aagtggccga tgcctcggcg atgggttggc
     3961 tggcgcgctt cttcaacagc ccgatctggc ccttctagcg agagtgaacc gatgcgatcg
     4021 attcgtcctg gactcctgtg cggactgctg ctgatggcgc tgtccagtgt cacgctggcc
     4081 gaccggctga aggatctggc cagtgtgtcc ggtgtgcgca ccaaccagct ggtgggctac
     4141 gggctggtgg tcggcctgaa tggcaccggc gacaaggcac ccttcaccga ccagaccttc
     4201 agcaacatga tggccaagtt cggcattgcg ctgccacccg gtatcgatcc aaagctgaag
     4261 aacgtcgcgg cggtggcgat ccatgcgcaa ctgccggcat tcgccaagcc gggccagagc
     4321 atcgatgtga ccgtctcctc gatcggcaat gccaagagtc tgcgtggcgg cagcctgctg
     4381 ctggcgccgt tgcgtggcac cgatggacag atctacgccg tggcgcaggg caatctggtg
     4441 gtggctggct tcgatgccag cggcaatgac ggttcacgca tcaccgtcaa cgtgccgagc
     4501 accggccgca ttcccagtgg cgccaccgtg gagcggatgg tgcagagcag cttcggacag
     4561 ggcgagagcg tggtcttcaa cctcaaccgt gccgacttca ccacggcacg acgggtcacc
     4621 gaggcgatca acagcttcat gggtccgcgt gccgccaccg caatcgacgc ctcgtcgatc
     4681 cgggtgatgg cgccgatcga catggcgcag cgggtcgaat tcctgtcgat gctggagaac
     4741 attgaagtca cgcccggcga tgccgccgcg cgggtggtga tcaactcccg caccggcacc
     4801 atcatcatcg gtcagcatgt ccaggtggca ccggcggcgg tgacccatgg cagcctgacg
     4861 gtcaccatct cggaaaatcc gcgggtcagc cagccgggtg ccttttcgcc gggacagacc
     4921 gtggtggtgc ctgacagcaa gatcgaggtc tccgaaggca atggccacat gttcaagttc
     4981 gatgccggcg tgtcgctcga caccatcgtg cgggcggtca atggtgtcgg cgcggcgccg
     5041 agcgacctga tggcgattct ggaggcgctg aaagaggccg gtgcgctgcg ggctgagctg
     5101 gtggtgatct gaggtgcggc gaccagcatg aaagtcgacc agagccccct ctacaacgat
     5161 ctggcctcgg tgcagcaact gggccggggc ggtgatccgc aggatccgca gcggctggct
     5221 gcggcggcgc ggcagttcga gtcgatgttc gtgcagatga tgaccaaggc gatgcgcgat
     5281 gcgaaccggg cattgatcgg tgacagcccc ttcagcagcc acgagagcga aatctaccag
     5341 gagatgctcg acaaccagtt gagcgtgatg aacggcagcg gcatcgggct ggccgaggtc
     5401 gtcctgcggc aactgcaagg cggcgagacc ggggcgcccg aggcgctggc gtcgatgggc
     5461 tacgcggcac cggcgcgcaa cccgcatctc gatctgcgct cggcccaact ggccatgcgc
     5521 caccgcgacc ccgcgccact ggaagggcca tcggcgccgc cgttcaaagt gccgcgactc
     5581 ggggtgcaga gcctgctctg ggatcaggat gcgaccggtt tcagctcgtc cgaagccttt
     5641 tcgcagcaac tctatccggc agcgcggcag gcggcctcca tgctgggcgt cgatccgcgg
     5701 ctgctgctgt cgcaggcggc gctcgaaacc ggctgggggc agcggatgat cagcggcaac
     5761 cggggcgaga acagcttcaa cctgttcggc atcaaggccg atgcaagctg gcaggggccg
     5821 aaggtgtggg tcgacacgct ggagtaccgc gacggcgtgg cagtcaggga gcgggccgcc
     5881 tttcgcgcct atggctccta tgaagagagt tttgccgact acgcccgctt cattcgcgag
     5941 cagccgcgct atcaggaggc gctgcgtcag gccggcaccc cgcgcggtta catccaggcg
     6001 ctgcaacagg caggttatgc caccgacccg gactatgcgc gcaaggtgat ggcgatctac
     6061 cgcggtgact cgatccagca ggcgccgctg gccatgaacc agtccgccgc tggcaccact
     6121 tcctatcagt gagaaccctg aaccatggcg ggtgatctgc tcaacatcag tctttcgggg
     6181 attcaggcca accggctcaa cctgtcggtc acgggtcaga acatcagcaa tgccaatgtc
     6241 gagggttaca gccggcagga ggccaatctt tcgacccgcc cggcgcagcg tgatggttcc
     6301 tttttcgttg gcagcggggt gacgatcgac caggtccgtc gtgttgccga ccagttcctg
     6361 acccgacaga tcatgctcga cagctcgcag aagcagcaga cgcaggcgat gcagagcggg
     6421 ctcgatgcgc tcgacaagat gctgtcgagc gaaaattccg ggctgaccac cgacctgaac
     6481 cgattctttc tgtcattcca ggcggtggcc cagtcgccat cgtcggtgac cgcgcgtgag
     6541 gtgctgctgg gtgatgcgcg tgggctggcg aaccgctttc agacgctggg gggcgacctt
     6601 ggccagcgca tcaaggatgt caatcagcaa ctcgatacct tgagcggcca gatcaacgag
     6661 ttgagcagtg ccattgcccg gctcaacatc aacatcgccg tgctctcggg caatggcagt
     6721 ggcgcacagc cgaacgacat gctcgatcag cgcgaccaac tgctgcgcaa actgtcggag
     6781 ctggtgcaga tcaacaccat cagccagagc gatggctcga tcaacgtcat gctcgacaag
     6841 ggcagcgcgc tggtggtggg cggacaggcc agcagcgtca gcagtgggcc tgatcccaac
     6901 gacaacgccc gttcactgct ctacctgcag ctgggcacgc agcggcagtc actcagcagc
     6961 ggcagtctgg gcgggcaact gggcggactg ctcgactacc gtgaccaggc gctggcaccg
     7021 acactgaccg aactggggcg catcggcggc agcattgcgc tgctggtcaa ccagcagcat
     7081 caactgggtg tcgacctgga aggccggatg ggcgggctgt tctttgccga ccccaacagc
     7141 gccacactgg tcgcccagcg ggtgcagccg aacctgaaca acccgccacc caaggatcag
     7201 gtgatggcgg tgcaactgct cgacccggca cagttcaaga gcagcgacta caagctcgac
     7261 ctgatcggcc cggccaacga ccagtacacc ctgacccggc tgagcgacaa tgtgcagatc
     7321 tccagcggca actttggcgc gctgccggcc agcatcagtg tcgatggagt gcagatcgat
     7381 ctgacctcgg gcaccttccg tgccggcgac cactacctga tcacgccgtt tcgcgccgtg
     7441 cccaactcca tggccatggc ggtctccaga ccacaaagcc tggccctggc gcaaccggtg
     7501 cgcggcagtg caaccctgac caaccagggc agcggggcgc tgaccacggt ggagatgacc
     7561 gaccgtggtc cctacgacgc cagcaccctg gcgctgccgt tgcgcatcac cttcaccagc
     7621 ggcactgcct acacggtgac cgacggcaac agcgccacgg tggcggccgg actgccctat
     7681 ccccccactg gcgccagcgg catgatcccc gcggcactgg gactcaaggt gacgatgagc
     7741 ggcagcccgg ttgctggcga cagcttcacc gtgcagttca acagtcaggc agtgggtgac
     7801 aaccgcaatg cactggcgct gggtgcgctg ggcaaccggc cgctgctgga gcagggcacc
     7861 ctggcactga tcgagagcga ccgggtgatg actggccgcg tcggcaccct cgcagcccgt
     7921 gccaaggtgg cgttcgactc cgccgatgcc tcgctgcggc agagccaggc ccagcgcgac
     7981 gcgatgtcgg gcgtcaacct cgatgaggag gccgccaaga tgatcgaatt ccagaacagc
     8041 tacaacgcct cggcccggct gattcaggtg gccagcgaac tgttcgacac cctgctcaac
     8101 tcagtgggcc gctgaccgag gagttgatcc gccatgcgca tctcgaccat gcagttctac
     8161 gcccaggcgc gcaacgccat tctcgataac cagagcaagc tgctggagac tcatgaccag
     8221 ctggcgaccg gcaagcgcgt cacccaggcc ggcgacgacg tcgtcgcctt cgtcgccatc
     8281 cagcgcctgc gggccgaaga gcagtccacc cagcgctacc tcgacaacat caccgccgcg
     8341 cgccagcgca tctcttcaga agacaccacg ctcgatcaga tcaccaacag cctgcaacgg
     8401 gtgcgcgaac tggcgatcca gagcgaaagc ggcgcgctga acacggccga ccgcgccggc
     8461 atcgccagcg aactgcgggc actgcgcgac cagcttctgg gtcagctcaa cagccgcgat
     8521 ggcgatggtc actacctctt ctccggctat caggccaaca ccgcgccctt tgccagcgac
     8581 gccagcggtg ccttctcgtt tcagggcgac gagggcattg ccgaactgac ggtcgctccg
     8641 ggtgtcacca tgccgctggg gcatgatggc cgctggctct tcgttgacct gctggcgccg
     8701 cgcaacgtca cggcgacggc ggccgccagc aataccggaa ccgtcaccac cgcgcagccg
     8761 atcgtcaccg accaggcgca ggtcgacggc tgggccggca agagcctgtc gatcgacttc
     8821 acctcggcca gccagttcgt catcaccgag cagccggcca acattccagt ggcgatccag
     8881 aaaggagtgc ccgccggcta tctgcccggc accccgatcg actacgtcgc cggcatggag
     8941 ctcgaattcg ccggcaccca gatcaaattg agcggcgcgc cgcaaagcgg cgacaggctc
     9001 accctgaccg ttgccgccgc cggccggcag gatctgctca ccagcgtcga tgccctggcc
     9061 cgaagcatca ccgacgccag tctcaacacg gcccagcgca gcgaagccat cgccgatgcg
     9121 attcagcaga tggatgcatc gcgcgaccgc atcagtcagg cccgcgccca ggtcggtgcc
     9181 cggctcaaca gcctcgacgc gctggagtcc aaccaccagc agagcaccct gcagaccaag
     9241 gaggtgctca ccaacctcga agacctcgac ttcaccgagg cgatcagccg cctcagccag
     9301 cagacggccg cgctgcaggc cgcacagcag agctatgcca ccctgagccg cctgtcgctg
     9361 ttcaacctgc tctcgtgagc gacagaacgg caatgatggg gcgtggtgcc gatcaactct
     9421 gcagcccggt gagcagcgtc accagctcgc cgatggcggt gtagggctcg cgcccgtcat
     9481 gggctgaaaa cctgtcggcc agcgcttgtg cgcgctgcgt ggnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nngaattggg gacagaccac ggttttctgg tttctgctcc
     9661 gctttggcag aaaaccgtgg ccgccaattc tcacttcggc aaacagtttt acggtggtga
     9721 tttggtgggc cttgcaggac ttgaacctgc aaccgtccat caatcccggg atggctcaga
     9781 ctgagcccgt ttttccgttt tcccttttga gtgaggcagg caatctgtct gatcggtgga
     9841 agaggccctg ccgccgcccc gaaccggcta caatttcacc cccgcaacag gttcaggatg
     9901 caggtcagtt gaagcagcag cacagccctt tgcccgctgc gggtgccgtg gagaagcaca
     9961 cgccgatgat gcaacagtat ctgcggctga aggtggagca ccccgatgcc ttgctgtttt
    10021 accgcatggg ggacttttac gagctgttct acgatgacgc ccggcaggcc gcgcaactgc
    10081 tcggcatcac gctgaccagc cgcgggcagt cggccgggga gccgattcca atggcgggtg
    10141 tgccgcacca tgcggccgag ggttatctgg ccaagctgac ccggctgggg ctgtcggtgg
    10201 cgatctgcga gcagatcggc gatcccgcca gcagcaaggg gccggtggag cgcaaggtgg
    10261 tgcgggtggt gacgcccggg accatcagtg atgaagcgct gctggagtcg gaccgcgaga
    10321 acctgctggt ggcgatcagc ggcgatggcc agcgcttcgg catcgccagc ctggagctga
    10381 gcagtggccg ctttcgcctc tgcgagcccg agtcgccgac ggcgctgatc gaagagctgg
    10441 gacggctgca gccggccgag ctgctgatcg atgagtcgtt gagcctcacg ttgcccaagc
    10501 cgtaccagcc gctgctgcgg cagcgtccga actgggagtt cgaccatgac agtgcccagg
    10561 ccgcattgac gcgccagttc cgcacccgga gcctggccgg tttcggctgc gaagggttgc
    10621 ggctggccat cggcgctgcc ggggcgctgc tgcactatgc ccgcgccacc cagcgcagcg
    10681 agctgcccca tctggagcgg ctggaggtgg agcaccgcga cgacagcgtg ctgctcgaca
    10741 gcgccacccg ccgcaacctg gagatcgacc gcaatctcgc cggtggacgc agcaacacgc
    10801 tgctgtcggt gctcgaccgg acggcgaccc cgatgggcag ccggctgctg agccgctggc
    10861 tgcatcggcc actgcgggat cagcagcagt tgggccatcg ccatctcgcc atcgagcagt
    10921 tgatcgatgg gcgccggcat gaagagatcc atgccctgct gcgccgggtg gatgatctgg
    10981 agcggattct gggccgggtg gcattgcgtt cggcgcgccc acgcgatctg acccggttgg
    11041 ggcaggcgtt gcggctgctg ccggatctgc atcggctgct gcatggcgtc gacgcgccgc
    11101 ggctgctggc gctcgatgcg gcggtcgggc tgttccctga actggtcgag ctgctggagg
    11161 cggcgctgct gccggagccg ccggtgctgc tgcgcgatgg cggcgtcatt gctcccggct
    11221 tcagcgccga gctcgatcag ttgcgcgcca tgggtcagga tgccggcgcc tttttgatgc
    11281 agatcgaact gcgcgagcgg gagcgcaccg ggctctccag cctcaaggtg cgctacaacc
    11341 gggtgcatgg ctactacatc gagatcagcc gcagtcaggc cgatctggcg ccgccggagt
    11401 atcagcgccg gcagagcgtc aagcatgccg agcgcttcat gattccggag ctgaagcagt
    11461 tcgaggagca ggcgctcagc agccagagcc gggcgctggc gctggagaaa atgctttacg
    11521 aagggctgct cgacagcctc aacctctcct tgggtccgct tcgggccagc gccgaggcgc
    11581 tggcggagct cgacctgctg gccaacctgg ccgagcgcgc ccacacgctc ggctatcagc
    11641 ggccgcaatt ctgcgcgcag ccgcgcatcg agatccgtca ggggcgccac ccggtggtgg
    11701 agcaggtcag cagtgacccc ttcatcgcca acgacctcga actcgacgac cagcggcgga
    11761 tgctgatcat caccggcccc aacatgggcg gcaagtcgac ctacatgcgg cagaacgcgc
    11821 tgattgcgct gctggcctac agcggcagct tcgtgccggc gcgcagcgcg attctggggc
    11881 cgatcgaccg catcttcacc cgcatcggtt cggccgacga cctggccggc gggcgctcga
    11941 ccttcatggt agagatgagt gagaccgcca acatcctgca ccatgccacc gccaacagcc
    12001 tggtgctgat ggatgagatc ggccgtggca ccagcacctt cgacggtctg gcgctcgcct
    12061 gggccagcgc ccgccatctg gccgaacggg ccggcgccct gaccctgttc gccacccact
    12121 acttcgagct gacggcgctg gccgagcagt tgccggcggt ggccaacgtg catctggccg
    12181 ccaccgaata tggcgagcgg atcgtctttc tgcatggcgt gcgcgagggg gcggccagcc
    12241 agagctacgg catccatgtc gcccggctgg ccggcctgcc gggtgcggtg atcaccgacg
    12301 ccgaggcccg gctgatgcag ctcgaggcgc tgtcgcgtgg ggcgcaggtg gcctcggccg
    12361 aaccaccgct gcagggtgac ctcttcgcgc cggtcgatct ggcccacagc gtgctcaagc
    12421 ggttgcaggc ggtcgatccc gaccggatga cgccacggca ggcgctggag tggctctatc
    12481 agctcaaggc gctgaccgag acatcgcgct gatcaccgcg acagaactgc ccgcagggtc
    12541 aacttctgct agaattttgc ccccatcgag gccgcattgg ctgttcatcc accttttcat
    12601 cccagcgtca gggagatatg catgacattc gtggtcactg agaactgcat caaatgcaag
    12661 tacaccgatt gcgtcgaggt ttgcccggtg gactgcttct acgaaggccc caactttctg
    12721 gtgatccacc ctgacgagtg catcgattgc ggtctgtgcg agcccgaatg tccggtggag
    12781 gcgatctttg acgaagacga gctgccggcc gggcaggagg agttcgtcga gatcaatgcc
    12841 cgtctggccg agagctggcc caacatcacc cacaaaaagg atgcgccggc cgacgccgac
    12901 gactggaaag gggtcaccgg caagcgcaag tacctcgaag agtagcgcct tgcactgcgc
    12961 agtgtcgcgg ctgattccga cgtgaacctc ggggtcggcc gccttctttg ctatcatgcc
    13021 gcaccaccct ctgccgaagc cccgccatgc acccgatgct gaccattgcc cagcgcgccg
    13081 cgcgccgggc cggcgaaaag atcgcctact ccattgaccg cctcagccag atcaaggtcg
    13141 aggagaaggg gcgtcatgac tatgtctccg agatcgacaa ggccgccgag gcgctgatca
    13201 tcgagacgct gcaacgcagc tatccggagc atggcattct gggcgaggag agcgggctga
    13261 tcgaggggcg cggtgccggc cgcgactacc tgtgggtgat cgacccgctc gacggcacca
    13321 ccaattttct gcatggcgtg ccgcactgcg cagtctcgat cggattgcgc catcgcggcc
    13381 agctcgacca ggcgatcgtg ctcgacccct tccggcagga ggagttcacg gccagccgcg
    13441 gcgccggcgc ccagctcaat ggacggcgga tacgggtcag cgaccgcaag agttttccgg
    13501 gctccctgct ggccaccggc tttccgttca agccggtcac cgcgcagacg cagggcaagg
    13561 tgagccacta ttttcgcacg ctggagcagt tggccggcgt cacgtccggc atccgccgcg
    13621 ccggttcggc cgcgctcgat ctggcctatg tggccgctgg ccgctatgac ggctactggg
    13681 agctcgatct gcaagcctgg gatctggccg ccggtgcgct gctggtacag gaggcgggcg
    13741 gactggtcag cgaccatgcc ggcggacaga actttctcga cggtggcgac atcgtcgcgg
    13801 ccgcgccgcg ctgccacaag gtgctgctcg acatgatccg ccatggcggt ggcgaaaaga
    13861 gctgacgggt cgcaaccggt ggcgaagcga aggagcgccc tgctcagtgc cgccgagtgt
    13921 cacgaactct ttcgccgcct gcaggcggcc aaccccaagc cgaccaccga gctgcactac
    13981 cgcaacccgt tcgagctgct gattgcggtg atgctctcgg cccaggcgac cgatgtcagc
    14041 gtcaaccggg tcaccccggc gctgttcgcc gccgcgccca ccgcgcagga gatggcccgg
    14101 cttggcagcg aggcgatcaa gggccatatc cgcaccctcg gcctcttcaa cagcaaggcc
    14161 gaacacatca gcgccacctg caggctgctg gtcgagcgcc atggcggcga ggtgcccgat
    14221 tcgcgcgcgg cactggaggc actgcccggc gtcggccgca agactgcgaa cgtggtgctc
    14281 aacaccgcct tcggcgagcc ggtgattgcg gtcgacaccc acctgtttcg cgtcgccaac
    14341 cgcaccggtc tggcgccggg caagagtgtc cagcaggtcg agcagggatt gatgaagcgg
    14401 gtgcccaagc cgtttctgca cgacgcccac cactggctga tcctgcatgg acgctacctc
    14461 tgcaccgccc gcaagccgca ctgcgaccgc tgtccgctgg ccgacctctg ccgcttcgag
    14521 gacaagcggc ttggcccccc atccgatcgg cctgagacca ctcaacccaa ccctgaggag
    14581 catccatgag cgacgccgtg ttcattcgtg aaggcgaatt tttccatccg caacccaatg
    14641 ccggcagtcc gtggggtccc gctaccctgc atggcgcagc gccggccggg ctgattggcc
    14701 acgcgctgga gcagctgctg ccttcgcccg agatgcagct ggcccggatg accatcgacc
    14761 tgttccgtcc ggtgccgatg gcgccgctgc ggttgagttc cgaggtggtg cgcgccggcc
    14821 gcaagatcca tgtggtccag gtctcgctgt gggccgatgc agtcgaggtc tgccgtgcca
    14881 gcggcgtcat gctgcgtcgc acgccgatcg atctgccagc caacctgttg ccggcagccg
    14941 acaagcccgc cggccccgag ggtctggcca tcaccggcat ccgcgatgcg gcggccggag
    15001 agtcagcctt tgcgctgctg cccggctttc atactgcgat cgaggtcaag cgggtcagcg
    15061 gcggcaaggg gcagggtgcc ggctgcgcct ggttgcgcat cccgatcccg ctgatcgatg
    15121 ggctcgaaac cactccgctg atgcggg
//
LOCUS       DAORCQ010000057        14917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2558_length_14917_cov_6.304602, whole genome shotgun sequence.
ACCESSION   DAORCQ010000057 DAORCQ010000000
VERSION     DAORCQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_14917_cov_6.304602"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKI23_07340"
     CDS             <1..551
                     /locus_tag="PKI23_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNN36788.1"
                     /translation="TWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMG
                     REPTPEELGERMEMPEDKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDNTVDLPIE
                     AATAESLKEATREVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIE
                     AKALRKLRHPSRSEHLRSFLDE"
     gene            580..656
                     /locus_tag="PKI23_07345"
     tRNA            580..656
                     /locus_tag="PKI23_07345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Ile,seq:cat)
     gene            complement(945..1340)
                     /locus_tag="PKI23_07350"
     CDS             complement(945..1340)
                     /locus_tag="PKI23_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36789.1"
                     /translation="MNPEQQTFLEDEFFSMTLMATVQRGKLYRVSSGEAEKDAFRRAL
                     RSELERLTEQYSVGVSEAQHLANIENLANSLSGSHPNALNAGRFRIGSAQKALNLHLK
                     YMWCLGLIPAPPHCPFDFMVLSRIPTVGT"
     gene            complement(1489..2013)
                     /gene="sodC"
                     /locus_tag="PKI23_07355"
     CDS             complement(1489..2013)
                     /gene="sodC"
                     /locus_tag="PKI23_07355"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HNN36790.1"
                     /translation="MRIPALFATCLLASTAALAAETSTPLNLVTAQGIGAAVGSVRIT
                     ETPYGLVFQPALQGLPPGLHGFHVHEHGTCMPSQKNGVAVAALAAGGHLDPKGSKKHG
                     EPWGDGHLGDLPPLHVAADGSATHPVLAPRLKLADVNGRALMLHAGGDNHADQPAPLG
                     GGGERMACGVIPQP"
     gene            complement(2121..2603)
                     /locus_tag="PKI23_07360"
     CDS             complement(2121..2603)
                     /locus_tag="PKI23_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HNN36791.1"
                     /translation="MQKEQRRQPADTTTPIETLLHDWLQRRQELTVLYVAVDGLRQFT
                     PKKVPITIKVQALCQALLDYLCAGHFKVFERLIEEFEHHDADSRALAARLLRQIDRST
                     AAAIAFNDIYDTEAHCAELLDALPEDLSRLGLVLAERFELEDQLIERLQQRQPQPSMA
                     "
     gene            complement(2705..3403)
                     /locus_tag="PKI23_07365"
     CDS             complement(2705..3403)
                     /locus_tag="PKI23_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNN36792.1"
                     /translation="MSALLPMPVTALALTLGGYLLALWMHRRLGRPVWLHPVLIAALP
                     IALLIGLGLLDAGDYRQASQPLVLLLGPATVALGLPLRRHWDEIRRLWGPLSIALLIG
                     VATATASAMLLADWLGLDRRLIATIAPKTATSPIAMEVARILGGEPGLAAGLVIITGI
                     IGATAGPVLFDWLGISDPRIRGFSLGLAAHGVGTARALDEGAQAGAFASLALGLTGTL
                     VALAIPTVLVYWFA"
     gene            complement(3400..3747)
                     /locus_tag="PKI23_07370"
     CDS             complement(3400..3747)
                     /locus_tag="PKI23_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNN36793.1"
                     /translation="MIRGLTLLFAAQWLGEVTTRLLQLPIPGAVLGMLLLMLLLRAAP
                     GLIDDAESAGGPLVSHLSLFFIPPLIAALFLVGLSASEWMRLLLAITLSTLGALVATA
                     LTLKWALARWPGR"
     gene            complement(3744..4244)
                     /locus_tag="PKI23_07375"
     CDS             complement(3744..4244)
                     /locus_tag="PKI23_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNN36794.1"
                     /translation="MLSWRYWLQTRPLNGLILLGCALLLAIALGLEHGLGLLPCNLCI
                     LQRIAFAAIGLTALLALLHNPARVGRKMYGLLMLIFADLGIALAGRQIWLQHLPPEQV
                     PACLPTIDYLVKVMPLNDVLLLALKGDGNCAEVTWRLLGLSIADWSLLAFVLFCGLAL
                     VQLLRR"
     gene            complement(4196..4882)
                     /locus_tag="PKI23_07380"
     CDS             complement(4196..4882)
                     /locus_tag="PKI23_07380"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNN36795.1"
                     /translation="MNHLAHFLLAGTEDEWRLGALLGDFRKGPIATPYPDLLSASIML
                     HRRIDAFCDRHPLTRQLIEHFPPALRRLGPILLDLGRDHFLSRHFTCFSPQPLADHAA
                     DIYALLEREQARLPAEAARFARLLAQHDLLCRYLDPAAVGATAARIIQRRLGPHQPVQ
                     QLERFWLAHYDQIGENFREFFPLLYDFAAIERERLRQAVIPPLPATHSEQIDRCSPGA
                     TGCKPVLSMA"
     gene            complement(4879..6465)
                     /gene="gshA"
                     /locus_tag="PKI23_07385"
     CDS             complement(4879..6465)
                     /gene="gshA"
                     /locus_tag="PKI23_07385"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNN36796.1"
                     /translation="MPNDNTDLSTFDSDELAPMLRGILRGIEKESLRIDRSGLLAPTR
                     HPAALGSALTHPLITTDYSEALIELITPTSTGIEATLTLLDEIHRCVYGALDQELLWC
                     ASMPCVLADDTQIPVAEYGNSNIGRLKHLYRIGLGHRYGRAMQTIAGIHYNFSLPDSL
                     WQALQARQGDRRSLTDYKTDRYFALIRNFRRHFWLLIYLFGAAPALCRSLVANRPHQL
                     ESFDDGSLYLPYATSLRMGSVGYQSDAQSSLTITHNDLDNYIDTLYGAMTTPYPAYQK
                     IGVKVDGEYRQINANLLQIENEYYSPIRPKRVANSGEKPLHALHQRGVEYVEVRCLDL
                     NPYLPLGIDAEQIRLLDIFLLNAALDDSPPISRDECHAIQHNQKTVVNEGRRPGVTLL
                     RAGSPVRLTDWGHALMEQMAPAAEQLDQSWGGDSYRQTLTRLTARLHDADLTPAARIL
                     AEMRDQRLSFSQFAMQHSLAHERHFRSRPLPPDRHAHFAELAEASWHAQQQIEASDQL
                     DFDSFLQAYQQQPIRDETRA"
     gene            complement(6568..8130)
                     /locus_tag="PKI23_07390"
     CDS             complement(6568..8130)
                     /locus_tag="PKI23_07390"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HNN36797.1"
                     /translation="MTTALSAKIEPAIYRDLSPAQLAEEAVRRGEGILASNGALVVST
                     GRRTGRSPVDRFIVEEPSTADAIHWGAINRPFNADDFDALWERVESYLADKDQFVSHL
                     HVGADPEHYIPTRVTTETAWHNLFGRNLFIQPGKYNPKGKGEWQIINAANFVCDPKRD
                     GTNSDGTVIIHFAQRKVLLAGMKYAGEMKKAMFAVQNFLLPEKDVLPMHCAANMGDDQ
                     SVTLFFGLSGTGKTTLSADPERYLIGDDEHGWGRGTVFNIEGGCYAKCINLSRENEPV
                     IWDAIKFGSVIENVVVNPLTREPDYADDSLTQNSRAVYPREHVERRVEENRAGEPKVI
                     IFLTCDLTGVIPPVSLLSKEAAAYHFLSGYTALVGSTEMGSGGGIKSTFSTCFGAPFF
                     PRPPSVYAELLMKRIEEFGSQVYLVNTGWTGGAHGRGGQRFSIPTTRAIIKAVQSGAL
                     LHVETELLPGLNLRVPTSVPGVDPKLLNPRNTWEDPAAYDAQARNLIQQFSDNFKKFT
                     VSDAILQAGPAL"
     gene            complement(8290..8712)
                     /locus_tag="PKI23_07395"
     CDS             complement(8290..8712)
                     /locus_tag="PKI23_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNN36798.1"
                     /translation="MVKKVSEPADAEPVRLDKWLWAARFFKSRTLAQEAISGGKVHYN
                     GQRVKPGREVQIGALLRIRLGWDEKEVVVRALSARRGAAPIAQQLYEETSASLAAREA
                     RGQMRQLDRLLAAPPPGRPTKRQRRQLHNLQDASDFEP"
     gene            complement(8706..9353)
                     /gene="yrfG"
                     /locus_tag="PKI23_07400"
     CDS             complement(8706..9353)
                     /gene="yrfG"
                     /locus_tag="PKI23_07400"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HNN36799.1"
                     /translation="MLDWKEIDTVLLDMDGTLLDLHFDNHFWVEHLPRRYAERHGMAL
                     TEAAPRLRQAVANQQGTLNWYCLDFWSRTLELDIVGLKREVAHLIALRGDTVAFLQRL
                     ATERKQRILITNAHRDSLDLKLLHTRLDQHLDALYSAHDFGQPKEQPTLWQSLQQVTP
                     FDPARTLLIDDNESVLRSAQQFGIRHLITIAQPDSQQPPRDGLAFPAIEQFSALW"
     gene            9443..10267
                     /gene="cysQ"
                     /locus_tag="PKI23_07405"
     CDS             9443..10267
                     /gene="cysQ"
                     /locus_tag="PKI23_07405"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNN36800.1"
                     /translation="MDQRALLEAVIDIACHAGDAILEIYQRDDHGVTDKADTSPLTAA
                     DLAAHHLIVAELARLTPEIPLLSEESGGITTAERRSWQRYWLIDPLDGTKEFINRNGE
                     FTVNIALIEAGCPVLGVVHVPVQQRTYAGAQGLGAQSRQSGQPARTIQVRPLAPRLAA
                     QQPVVVVGSRRHGTEQLEACIERIRAKLGPVELTNMGSSLKICLVAAGEADLYPRLGP
                     TCEWDTAAAQAVLEAAGGMLTDPQLQPLRYNQKEELLNPFFYAMGDRGIDWRSVLL"
     gene            complement(10326..11390)
                     /gene="fba"
                     /locus_tag="PKI23_07410"
     CDS             complement(10326..11390)
                     /gene="fba"
                     /locus_tag="PKI23_07410"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017216575.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNN36801.1"
                     /translation="MALVSMRQLLDHAAEHGYGVPAFNVNNLEQMRAILEGASETDSP
                     VIVQASAGARKYAGANFLRHLILAAVEEFPHIPVVMHQDHGTSPAVCQRSIQLGFSSV
                     MMDGSLGEDGKTPTDYDYNVAVTRRVVEMAHACGVSVEGELGCLGSLETGTAGKEDGI
                     GADGVLSHDQLLTDPDEAARFVKETEVDALAIAIGTSHGAYKFSRPPTGDILAIDRIK
                     AINARIPDTHLVMHGSSSVPQEWLAIINEYGGEIPETYGVPVEEIQAGIKHGVRKINI
                     DTDLRLASTGAIRRYLAQHPSEFDPRKYLSETVKAMKSIVKARYEAFGTAGQASKIKP
                     VSLELFYQRYQRGELKPRIQ"
     gene            complement(11434..12597)
                     /locus_tag="PKI23_07415"
     CDS             complement(11434..12597)
                     /locus_tag="PKI23_07415"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNN36802.1"
                     /translation="MTFKRMLDLDLSGKRVLIREDLNVPIKQGRIGSDARLLASLPTL
                     RAALDAGARVMVLSHLGRPEEGKFDPELSLAPVAARLGELLGREVPLVRDWLDGVEVA
                     AGSLVLCENVRFNKGEKKDDPVLAQKMAALCDIFVMDAFGTAHRAEASTHGVVQHAPV
                     ACAGPLLAAELDALALALKNPQRPLLAIVGGSKVSTKLTVLDSLSQKVDRLIVGGGIA
                     NTFLAAAGYPVGRSLFEADLLDEARRLMANGQIPLPSDVVVATELSAEARATIKPVDA
                     VAENEMILDIGPVTAASYAEIIQQMGTVLWNGPVGVFEHEQFGHGTRTVAEAIAASTA
                     FSLAGGGDTLAAIDKYGIGERLSYISTGGGAFLEYVEGKTLPAVAALELRATR"
     gene            complement(12635..14638)
                     /gene="tkt"
                     /locus_tag="PKI23_07420"
     CDS             complement(12635..14638)
                     /gene="tkt"
                     /locus_tag="PKI23_07420"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNN36803.1"
                     /translation="MSRSDALANAIRFLSMDAVEQAKSGHPGAPMGMADMATVLWRNH
                     LRHDPRQPQWADRDRFVLSNGHASMLLYSLLHLSGYDVTLDDLRRFRQLDSKTAGHPE
                     YLHCPGVETTTGPLGQGLANAVGMALAERLLAEQFNRPGFDIVDHRTWVFVGDGCLME
                     GISHEACSLAGTLGLGKLTLFYDDNGISIDGEVEGWFTDDTAARFRAYGWQVLGPIDG
                     HDANAIEAAIQTAKAEATRPALIICKTTIGFGAPTKAGTESVHGAPLGATEVAAARQQ
                     LNWPHPPFVIPEEIRVAWDARASGAALARQWQALFAAYRDAHPELAAGFERRQSGALP
                     SDFLAQANHHIESLQQQGVTVASRKASQMALEAYSPQLPELLGGSADLTGSNLTNWSG
                     SQSIGADNGGNYLHYGVREFAMGAMMNGIALHGGFIPYGGTFLVFLDYARNAVRMAAL
                     MRQRLVWVFTHDSIGLGEDGPTHQPIEQLSGLRSTPNLSTWRPCDAVESAVAWREALL
                     RRDGPTALALSRQNLPHQPRDAAQLAAIGRGGYILHEPAQPPRAVVIATGSEVALAMR
                     AAQQLDERALPVRVVSMPSTDRFLAQSSDYRDQVLPAGVPRIAVEAAHPDCWYRFVGL
                     EGRVIGINDFGKSAPAEQLFQHFGITVERVVATVLELTGQSSS"
BASE COUNT         2627 a         4529 c         5128 g         2633 t
ORIGIN      
        1 ccacctggtg gattcgccag gccatcaccc gctcgatcgc cgaccaggcg cgcacgatcc
       61 gcattccggt gcacatgatc gagaccatca acaagctcaa ccgcatctcc cggcagatgc
      121 tgcaggagat gggacgggag ccgacacccg aagagctggg cgaacgcatg gagatgcccg
      181 aagacaaggt gcgcaaggtg ctgaagatcg ccaaggagcc gatctcgatg gagacgccga
      241 tcggcgatga tgaagattcg cacctcggtg acttcatcga ggacaacacg gtcgatctgc
      301 cgatcgaggc cgccaccgcc gagagcctga aggaggccac ccgcgaggtg ctggccgggc
      361 tgaccgcacg ggaggccaag gtgctgcgga tgcgcttcgg catcgacatg aacaccgatc
      421 acacgctcga ggaggtcggc aagcagttcg acgtcacccg cgagcgaatc cggcagatcg
      481 aggccaaggc gttgcgcaaa ctgcgccacc cctcccgctc cgagcatctg cgcagttttc
      541 tcgacgagtg aaccggcagc gccggtcgac gaagtctccg ggcctttagc tcagttggtc
      601 agagcgctcg actcataatc gattggtcgc aggttcaagt cctgcaaggc ccaccaaatc
      661 accaccgtaa aactgttggc cgaagtaagg attgggggta cggttttctg ccaaagtgga
      721 gcggaagccg aggaaaccgg ggtctgtccc caattctccc aattctccat tctcaacgtg
      781 ctagctcagg ggcgcgccgc ttgcggcgca tcccgctgga gcgacgggct gggcggcgtt
      841 gtacaagcgc aactcccact cggcaagggg gctgttgcct gccgccgttt tggcaagaca
      901 gacaattcgc tcgtattcgg gtaaagagtc aagttgtgtc catctcacgt tccgacggtt
      961 gggatgcgcg acaagaccat aaaatcaaac gggcagtgcg gcggtgccgg aatcagtcct
     1021 aggcaccaca tgtacttgag gtggagattg agcgcttttt gcgcggagcc gattctgaac
     1081 cgtcctgcat tcagagcatt ggggtgcgaa ccagataggg agtttgcaag gttctcgatg
     1141 ttcgcaaggt gctgcgcttc cgaaacgccg acactatatt gctcggtgag acgctccagt
     1201 tctgagcgca gagcacggcg gaatgcatcc ttctcggctt cgcccgagct tactcggtat
     1261 agttttccac gctgcactgt agccatcagc gtcatgctga aaaattcatc ttcaaggaag
     1321 gtttgctgtt ccgggttcat ttccgacacg cccgactaga attagacagc ccagtttttc
     1381 cggatggata atcccgttcc cgccttggag acagaccgcg gtttcctcag gcattgcggc
     1441 tggcgtccat ccgtgcgccc attcatgcaa caatgagcgc cggccacgct atggctgggg
     1501 gatgacgcca caggccatgc gctcgccgcc gccaccgagt ggcgcgggct ggtcggcgtg
     1561 gttgtcgccg ccggcgtgca gcatcagcgc gcggccgttg acatccgcca gcttgagtcg
     1621 gggggccagt accgggtggg tggcgctgcc atctgcggcg acatggagcg gcggcaggtc
     1681 acccagatgg ccatcgcccc agggctcgcc atgcttcttg ctgcctttgg ggtcgaggtg
     1741 gccacccgcc gccagcgccg ccactgcgac accatttttc tgcgacggca tgcaggtgcc
     1801 atgctcatgc acatgaaagc cgtgcaggcc cggtggcagt ccctgcaggg ccggctggaa
     1861 caccagtcca tagggggttt cggtgatgcg cacgctgccc acggcggcgc cgatgccctg
     1921 ggccgtcacc aggttgaggg gggtgctggt ctcggcagcc agcgccgcgg tgctggcgag
     1981 caggcaggtg gcgaagaggg cgggaatgcg catggggggc tccttgtgtg cgagggaaat
     2041 tccaggtcgt gttccattca tccagccgca ggtggacatc aggccactga tcgacgagac
     2101 ggtccgccgc cagcgtgcgc tcaggccatg ctgggctgcg gttgccgttg ttgcagccgt
     2161 tcgatcaact ggtcttcgag ctcgaaccgt tcggccagca ccaagccgag acgcgacaga
     2221 tcctccggta gggcatcgag cagttcggcg cagtgggcct cggtgtcgta gatgtcgttg
     2281 aaggcgatgg cggcggcggt gctgcggtcg atctggcgca gcagccgggc ggccagggcc
     2341 cggctgtcgg cgtcgtggtg ttcgaactcc tcgatcagcc gctcgaacac cttgaagtgg
     2401 ccggcgcaga ggtagtcgag cagtgcctga cagagcgcct gcaccttgat ggtgatcggc
     2461 accttcttgg gggtgaactg ccgcagacca tcgacggcga catagagcac ggtcagttcc
     2521 tggcgtcgtt gcagccagtc gtgcagcagg gtttcgatcg gagtggtcgt gtcggccggt
     2581 tgccgtcgtt gttctttctg catgggttgc acctgggtcg aatcctgaaa ggcgtgccga
     2641 tcgctggcga gcgccggggt tggccagtct acaagaaacc gtgaatgtga cctacttcac
     2701 agcctcatgc aaaccaatag accagaacgg tggggattgc cagcgcgacc agcgtgccgg
     2761 taaggccaag ggcgaggctg gcgaaggcgc cggcctgagc accctcgtcg agggcgcggg
     2821 cggtgccgac gccatgggca gccagaccga ggctgaagcc gcgaatgcgt gggtcgctga
     2881 tgccgagcca atcgaacagc accggcccgg cggtggcgcc gatgatgccg gtgatgatga
     2941 ccagaccggc ggccagaccc ggttcgccgc cgagaatgcg tgccacctcc atggcgatcg
     3001 gcgaggtggc ggtcttgggc gcgatggtgg cgatcagccg gcggtcgagt ccgagccagt
     3061 cggccagcag catggcgctg gcggtggcgg tggccactcc gatcagcagc gcgatgctga
     3121 gcggccccca gagccggcga atctcatccc agtggcggcg cagcggcaga ccgagcgcga
     3181 ccgtggccgg tcccagcagc agcaccagcg gctggctggc ctggcggtag tcgccggcgt
     3241 cgagcaggcc gagtccgatc agcagcgcga tcggcagggc ggcgatcagc accggatgga
     3301 gccagaccgg ccgtcccagc cggcgatgca tccacagcgc cagcaggtag ccgccgagcg
     3361 tcagcgcgag ggccgtcacc ggcatcggca gcagcgcact catcgaccgg gccagcgcgc
     3421 cagcgcccat ttgagggtga gggcggtcgc gaccagcgcg cccaaggtgc tgagggtgat
     3481 ggcgagcagc agccgcatcc attcactggc ggagagcccg accagaaaga gcgcggcgat
     3541 cagtggcggg atgaagaaca gcgacagatg gctgaccagc gggccaccgg cgctctcggc
     3601 atcatcgatc aggccgggtg ccgcgcgcag caacagcatc agcagcaaca tgccgagcac
     3661 tgcaccggga atcggcagct gcagcaggcg ggtggtgact tcgcccagcc actgggcggc
     3721 gaacagcagg gtgaggccac ggatcatcgc cgcagcaact gcacgagggc aaggccgcaa
     3781 aagagcacga aggcgagcag cgaccagtcg gcgatcgaca acccgagcag gcgccaggtc
     3841 acctcggcgc agttgccatc gcccttgagt gccagcaaca gcacgtcgtt gagcggcatc
     3901 accttcacca ggtagtcgat ggtcggcaga caggccggca cctgctccgg tggcaggtgt
     3961 tgcagccaga tctgccgccc tgccagcgcg atgccgaggt cggcgaagat cagcatcagc
     4021 aggccataca tcttgcggcc gacccgtgcc gggttgtgca gcagcgccag cagtgcggtc
     4081 aggccgatgg cggcgaaggc gatccgttgc aggatgcaga gattgcaggg cagcaggccg
     4141 aggccatgtt ccagcccgag ggcaatggcc agcagcagcg cacagccgag caggatcagg
     4201 ccattgagag gacgggtttg cagccagtag cgccaggaga gcatcggtcg atctgttcgg
     4261 aatgggttgc agggagcggc ggaatcaccg cctgtcgcag gcgttcgcgc tcaatggccg
     4321 cgaagtcata cagcagcggg aaaaattcgc ggaaattctc gccgatctgg tcgtaatgcg
     4381 ccagccagaa ccgttcgagt tgctgcaccg gctggtgtgg acccagtcgc cgctgaatga
     4441 tgcgcgccgc tgtcgcgccg actgcggcgg ggtcgagata gcggcagagc aggtcgtgct
     4501 gtgccagcag acgggcgaaa cgggctgcct cggccggcaa ccgcgcctgc tcgcgctcca
     4561 gcagtgcata gatgtcggcg gcatggtcgg ccagcggctg cgggctgaaa caggtgaagt
     4621 ggcggctgag gaagtggtcc cggccaaggt cgagcagaat cggacccagg cggcgcagtg
     4681 ccggcggaaa gtgttcgatc agttgccgtg tcagcggatg gcggtcgcag aaggcatcga
     4741 tgcgccggtg cagcatgatg ctggcactga gcaggtcggg gtagggggtg gcgatcggtc
     4801 ccttgcgaaa gtcgcccagc agtgccccca gtcgccactc atcctcggtg ccggccagca
     4861 ggaagtgggc cagatggttc atgcccgggt ttcatcgcga atcggctgtt gctgataggc
     4921 ctgcaggaag ctgtcgaaat cgagctggtc gctggcttcg atctgctgct gcgcatgcca
     4981 ggaggcctcg gccagttcgg cgaaatgggc gtgccggtcc ggcggcagcg gccggctgcg
     5041 aaagtgccgt tcgtgcgcca gggagtgctg catggcgaac tgcgagaagc tgaggcgctg
     5101 atcgcgcatc tcggccagga tgcgtgcggc cggggtcagg tctgcatcgt gcagccgggc
     5161 ggtgagccgg gtgagggtct ggcggtagct gtcgccaccc cagctttggt cgagctgttc
     5221 cgcggcgggc gccatctgct ccatcagcgc atggccccag tcggtcagtc gcaccgggct
     5281 gccggcgcga agcagggtca ctccgggacg gcgtccctcg ttgacgacgg ttttctggtt
     5341 gtgctggatg gcgtggcact cgtcacggct gattggcgga ctgtcgtcaa gggcggcgtt
     5401 cagcagaaag atgtcgagca ggcgaatctg ctcggcatcg atgccaagcg gcaggtaggg
     5461 gttgagatcg aggcagcgca cctcgacata ctccacgccg cgctggtgca gcgcatggag
     5521 cggtttttca cccgagttcg ccacccgttt ggggcggatc gggctgtagt actcgttttc
     5581 gatctgcagc aggttggcgt tgatctgccg gtactcgcca tcgaccttga cgccgatctt
     5641 ctgataggcg ggataggggg tggtcatggc accgtagagc gtgtcgatgt agttgtcgag
     5701 gtcgttgtgg gtgatggtca ggctcgactg ggcatcgctc tggtagccga cgctgcccat
     5761 gcgcagcgag gtggcgtaag gcaggtagag cgagccgtca tcgaaggatt cgagctgatg
     5821 tggccgattg gcgaccaggc tgcgacacag cgccggggcc gcaccgaaca gatagatcag
     5881 cagccagaag tgtcggcgga agttgcggat cagtgcaaag tagcggtcgg tcttgtagtc
     5941 ggtcagcgag cgccggtcgc cctgccgcgc ctgcagggcc tgccacaggc tgtcgggcag
     6001 cgagaagttg tagtggatgc cggcaatggt ctgcatcgcc cggccgtagc ggtggcccag
     6061 gccgatgcgg tagaggtgct tcaggcggcc gatgttcgag ttgccatact ccgccaccgg
     6121 aatctgcgtg tcgtcggcga gcacgcaggg catgctggca caccagagca gctcctgatc
     6181 gagggcgcca tagacacagc ggtggatctc gtcgagcagt gtcagcgtcg cctcgatgcc
     6241 ggtcgaggtg ggggtgatca gctcgatcag cgcttcggag tagtcggtgg tgatcagcgg
     6301 atgggtcagc gccgagccca gcgcggccgg atggcgggtc ggcgccagca atccgctgcg
     6361 gtcgatgcgc aggctctcct tctcgatgcc gcgaagaatg ccgcgcaaca tcggcgccag
     6421 ctcatcgctg tcgaacgtgg agaggtcggt gttgtcattg ggcacggtca aactccagcg
     6481 cgagtgttga gccggagact ctaccacggc cacgggtgat tcgacctgct ggagaactcc
     6541 gcaggggagt cctccagggc atggcggtta cagagcgggg cccgcctgca ggatggcgtc
     6601 ggagacggtg aacttcttga agttgtcgct gaactgctgg atcaggttgc gtgcctgcgc
     6661 gtcataggcg gcagggtctt cccaggtgtt gcgcgggttc agcagctttg gatcgacgcc
     6721 cggcacactg gtgggaacgc gcagattgag tcccggcagc agttcggtct cgacatggag
     6781 cagcgcgccg gactgcaccg ccttgatgat ggcgcgggtg gtggggatgc tgaagcgctg
     6841 gccgccgcgg ccatgggcgc cgccggtcca gccggtgttg accagataga cctggctgcc
     6901 gaactcctcg atccgcttca tcagcaactc ggcgtagacg ctcggtggcc gcggaaagaa
     6961 cggcgcgccg aagcaggtgg agaaggtcga cttgatgccg ccgcccgagc ccatttctgt
     7021 ggagccgacc agcgcggtgt agccactcag gaagtggtag gcggcggcct ctttcgacag
     7081 cagcgacacc ggcggaatca cgccggtgag gtcgcaggtc agaaagatga tcaccttcgg
     7141 ttcgccggcg cggttctcct cgacccgtcg ctcgacatgc tcgcgcggat agaccgcccg
     7201 gctgttctgg gtcagggagt catcggcata gtcgggttcg cgggtgagcg ggttgaccac
     7261 cacattttcg atcaccgagc cgaacttgat cgcatcccag atcaccggct cgttctcgcg
     7321 gctcaggttg atgcatttgg catagcagcc gccctcgatg ttgaagacgg taccacgacc
     7381 ccagccatgc tcgtcatcac cgatcaggta gcgttccgga tcggccgaca gggtggtctt
     7441 gccggtgccg gagaggccaa agaagagcgt caccgactga tcatcgccca tgttggcggc
     7501 gcagtgcatc ggcagcacgt ctttttcggg cagcagaaaa ttctgcaccg cgaacatcgc
     7561 ctttttcatc tcgccggcgt acttcatgcc ggccagcagg accttgcgct gggcgaagtg
     7621 gatgatgacg gtgccatcgc tgttggtgcc gtcacgcttg ggatcgcaga cgaagttggc
     7681 ggcattgata atctgccact cacccttgcc cttggggttg tattttccgg gctggatgaa
     7741 caggttgcgg ccaaagaggt tgtgccaggc ggtttcggtg gtcacccgcg tcgggatgta
     7801 atgctccgga tcggcaccga catgcaggtg agagacgaac tggtccttgt cggccaggta
     7861 gctctcgacc cgttcccaca atgcatcgaa atcatcggca ttgaatggcc ggttgatcgc
     7921 cccccagtgg atggcatcgg cggtgctggg ctcttcgacg atgaagcggt cgactggcga
     7981 gcgaccggtg cgtcggccgg tggagacgac cagtgcgcca ttgcttgcaa ggatgccctc
     8041 accccgtcgg acggcctctt cggccagttg ggcggggctg aggtcgcggt aaatcgcggg
     8101 ttcgatcttg gcggagagcg cagtggtcat gggcttactc gatcctgtat gcggtgggcg
     8161 gtgaatgaac tggcgcaggt aaagcggctg aggggcacga tgcgggatgg tgtgatcggt
     8221 cgggaaggcg ccatggccgg caattatggc aaaagcggcc ctgctgtgcc atgtttgcgg
     8281 ccgctgccat catggctcaa agtctgaagc atcttgcaga ttgtgcaact gccgccgctg
     8341 ccgtttggtg gggcgacccg gcggtggggc ggccagcaac cgatcgagct ggcgcatttg
     8401 gccacgcgct tcacgggcag cgaggctggc ggaggtctct tcatagagct gttgggcaat
     8461 gggtgcggcg ccgcgtcgtg ccgacagcgc gcgcaccacc acctccttct catcccaacc
     8521 cagacggatg cgcagcaggg cgccgatctg cacctcccga cccggtttga cccgctggcc
     8581 gttgtagtgg accttgccgc cactgattgc ctcctgggcc agcgtccggc ttttgaagaa
     8641 gcgtgctgcc cacagccatt tgtcgagccg gaccggttcg gcgtcggcag gttcgctgac
     8701 cttttttacc atagggccga gaactgctcg atcgccggaa aggcaagccc gtcgcgaggg
     8761 ggctgctgac tgtccggctg ggcgatggtg atcaggtggc gaatgccgaa ctgctgcgcg
     8821 ctgcgcagca ccgactcgtt gtcgtcgatc agcagggtgc gggccggatc gaacggtgtc
     8881 acctgttgca gcgattgcca gagggtcggc tgttccttgg gctggccgaa atcgtgggcg
     8941 ctgtagagcg catcgaggtg ctgatcgagc cgggtgtgga gcagcttgag gtcgaggctg
     9001 tcgcggtggg cattggtgat caggatgcgc tgcttgcgct cggtggccag tcgctgcaga
     9061 aaggccacgg tatcaccacg cagtgcgatc aggtgggcca cctcgcgctt gagtccgacg
     9121 atgtcgagct ccagcgtgcg cgaccagaag tcgagacagt accagttgag cgttccctgc
     9181 tggttggcca ccgcctgccg cagccgtggc gccgcctcgg tcagcgccat gccatgccgt
     9241 tcggcatagc ggcgcggcag atgttcgacc cagaagtggt tgtcgaagtg caggtcgagc
     9301 agggtgccat ccatgtcgag cagtacggtg tcgatctctt tccagtcgag cattcggtca
     9361 atccggcggt tggcaagggc ggttttgctg ttaggcttgt gcgggcaagc cttttgaacc
     9421 tccgcattct acaggaacca ccatggatca acgcgccctg ctcgaagctg tcattgacat
     9481 cgcctgccat gccggcgacg cgatactcga aatctatcaa cgcgatgacc acggcgtgac
     9541 cgacaaggcc gacacctcgc cgctgacggc tgctgacctg gcggcgcacc acctgatcgt
     9601 ggccgagctg gcgcggttga cgccggagat tccactgctt tccgaggagt cggggggtat
     9661 cacgacagca gagcgccgaa gctggcagcg ttactggctg atcgacccgc tcgatggcac
     9721 caaggagttc atcaaccgca atggcgaatt cactgtcaat atcgcgttga tcgaggcggg
     9781 ttgtccggtg ctcggtgtgg tccatgtgcc ggtgcagcaa cgtacctatg ccggcgcgca
     9841 ggggctgggt gcgcagagcc ggcagtcggg ccagccggcg cggacgattc aggtccggcc
     9901 gctggcgcca cggctggcgg cacagcagcc ggtggtggtg gtcggcagtc gccggcatgg
     9961 caccgagcaa ctggaggcct gcatcgagcg cattcgcgca aaactggggc cagtggagct
    10021 gaccaacatg ggcagctcgc tgaagatctg tctggtggcg gccggtgagg ccgacctcta
    10081 cccccgtctc ggtcccacct gtgagtggga caccgctgcg gcccaggcgg tgctcgaagc
    10141 cgccggcggc atgctgaccg acccgcagtt gcagccattg cgctacaacc agaaagagga
    10201 gctgctcaat cccttcttct acgccatggg cgaccgcggc atcgactggc ggtcggtgct
    10261 gctctgagcc tggtcgccct cctgccgggg ccatcagggg ggatggcctc ggcagggtgg
    10321 aaggctcact ggatgcgcgg cttcagctcg ccccgctgat agcgctggta gaacagctcg
    10381 agcgagaccg gtttgatctt cgaggcctgc ccggcggtgc cgaaggcttc atagcgcgcc
    10441 ttgacgatcg acttcatcgc cttgacggtc tcgctgaggt atttgcgcgg atcgaactcg
    10501 gacgggtgct gcgccaagta gcggcgaatg gcgccggtcg aggcgagccg caggtcggta
    10561 tcgatgttga tcttgcgcac gccatgcttg atgccggcct ggatctcttc gaccggcacg
    10621 ccgtaggtct ccggaatctc gccgccatac tcgttgatga tcgccagcca ctcctgcggc
    10681 accgaggagg agccatgcat caccagatgg gtgtcgggaa tgcgggcgtt gatcgccttg
    10741 atgcgatcga tggcgagaat gtcaccggtc ggtgggcggc tgaacttgta ggcgccatgg
    10801 ctggtgccga tggcaatggc cagcgcgtcg acctcggtct ccttgacgaa gcgggcagcc
    10861 tcatcggggt cggtcagcag ttggtcatgg ctcaatacgc catcggcgcc gatgccgtcc
    10921 tcctttccgg cggtgccggt ctccagtgaa ccgagacagc ccagttcgcc ctcgaccgaa
    10981 acgccgcagg cgtgggccat ctccaccacc cggcgggtga cggccacatt gtagtcatag
    11041 tcggtggggg tcttgccatc ctcgcccagc gagccatcca tcatcaccga tgagaagccg
    11101 agctggatcg agcgctggca gacggccgga ctggtgccgt ggtcctgatg catcaccacc
    11161 gggatgtgcg gaaactcctc gaccgccgcc agaatcaggt ggcgcagaaa gttggcgccg
    11221 gcatatttgc gggcgccggc cgatgcctgc acgatgaccg ggctgtcggt ctcgctggcg
    11281 ccttcgagaa tcgcccgcat ctgctccagg ttgttgacgt tgaaggcggg tacgccgtaa
    11341 ccatgctcgg cggcgtggtc cagcagttga cgcatgctga ctagggccat gatcgttcac
    11401 ctttggttga ggggtgggtg gattgggtga tcatcatcgg gttgcgcgca gctcgagtgc
    11461 cgccacggcg ggcagtgtct tgccttcgac atattcaaga aaggcgccgc caccggtgga
    11521 gatgtaggag agccgctcgc cgatgccata tttgtcgatt gccgccagcg tatcgccccc
    11581 gccggccagt gaaaaggccg tgctggcagc aatggcttcg gccactgtcc gggtgccatg
    11641 gccgaactgc tcatgttcaa agacgccgac cgggccattc cagagcaccg tgcccatctg
    11701 ctgaatgatc tcggcatagc tggcggcagt gaccgggccg atgtcgagga tcatctcatt
    11761 ttctgcgacg gcatcgaccg gcttgatggt ggcgcgggct tcggcgctca actcggtggc
    11821 caccaccacg tcgctcggca gtggaatctg cccattggcc atcagccgcc gcgcttcgtc
    11881 gagcagatcg gcctcgaaca gcgagcggcc caccgggtag ccggcggcgg ccagaaaagt
    11941 gttggcgatg ccgccgccga cgatcagccg gtcgaccttt tgcgacagtg aatcgagcac
    12001 cgtcagcttg gtcgacacct tggagccacc gacgatcgcc agcagcggcc gctgcggatt
    12061 cttgagcgcc agcgccagtg catccagctc ggcggccagc aatggaccgg cacaggcgac
    12121 cggggcatgc tgcaccacgc catgggtcga ggcttcggcg cggtgggcgg tgccgaaggc
    12181 atccatcacg aagatgtcgc acagtgccgc catcttctgt gccaggaccg gatcatcctt
    12241 cttctcaccc ttgttgaaac ggacgttctc gcacagcacc aggctgcccg cagccacctc
    12301 gacaccgtcg agccagtcac gcaccagcgg cacctcgcgc ccgagcagct cgccgagccg
    12361 ggcggcgacc ggtgccagcg acagttccgg gtcgaacttg ccctcttcgg ggcggcccag
    12421 atgcgacagc accatcaccc gggcgccggc gtcgagcgct gcacgcagtg tcggcaggct
    12481 tgccagcagg cgggcgtcac tgccgatgcg cccctgcttg atcggcacat tgaggtcttc
    12541 gcgaatcagt acccgcttgc cgctgaggtc aaggtcgagc atccgtttga atgtcatgcc
    12601 aaagctcctt ggaatggatc aggtctggtc tggattagct gctcgactga ccggtcaact
    12661 ccagcacggt cgccacgacg cgttcgacag tgatgccgaa gtgctggaac agctgttcgg
    12721 cgggcgccga cttgccgaaa tcgttgatgc cgatcacccg accctcgagc ccgacaaaac
    12781 gataccagca gtcaggatgg gctgcctcca ccgcgatgcg cggcacgccg gccggcagca
    12841 cctggtcacg gtagtcgctg ctctgggcca gaaaacggtc ggtcgaaggc atcgacacca
    12901 cccgcaccgg cagggcgcgc tcgtcgagct gttgcgccgc acgcatcgcc agcgccacct
    12961 cagaaccggt ggcgatcacc acggcccgcg gcggttgtgc cggctcatgg aggatgtagc
    13021 cgccgcgacc aatcgcggca agctgtgcgg catcgcgcgg ctggtgcggc agattctgcc
    13081 ggctcagtgc cagcgccgtc gggccgtcgc ggcgcagcag tgcctcgcgc caggccactg
    13141 cgctctcgac cgcgtcacag gggcgccagg tcgagaggtt gggcgtgctg cgcagaccgg
    13201 acaactgctc gatcggttgg tgggtcgggc catcctcacc caggccgatc gaatcatggg
    13261 taaagaccca caccagccgc tgccgcatca gtgccgccat gcgcaccgcg ttgcgggcat
    13321 agtcgagaaa caccaggaag gtgccgccgt aagggatgaa tccgccatgc agcgcgatgc
    13381 cgttcatcat cgcgcccatc gcaaattcgc ggacaccata gtgcaggtag ttgccgccgt
    13441 tgtcggcgcc gatcgattga ctgccggacc agttggtgag gttggagcca gtcaggtcgg
    13501 ccgaaccgcc cagcagttcg ggaagctgcg gcgagtaggc ctcgagcgcc atctgcgacg
    13561 ccttgcggct ggcgacggtc accccttgct gttgtagcga ttcgatgtgg tggttggcct
    13621 gggcaagaaa gtcgctgggc agtgcgccac tctgtcgccg ctcgaatccg gcggccagtt
    13681 ccgggtgggc gtcgcggtag gccgcgaaca gcgcctgcca ctgccgcgcg agcgccgcgc
    13741 cgctggcgcg ggcatcccag gccacgcgaa tctcctccgg aatcacgaat ggcggatggg
    13801 gccagttcag ttgctggcgc gctgccgcca cctccgtcgc gcccagcggt gcgccatgca
    13861 ccgactcggt tccggccttg gtgggtgcgc cgaagccgat ggtggttttg cagatgatca
    13921 gtgccgggcg ggtggcttcg gccttggcag tctggatcgc cgcttcgatg gcgttggcgt
    13981 cgtggccatc gatcgggccc agcacctgcc agccataggc gcgaaaacgg gccgcggtgt
    14041 cgtcggtgaa ccagccctcg acctcgccat cgatggagat gccattgtca tcataaaaaa
    14101 gggtcagctt gcccagcccg agggtgccgg ccagtgagca ggcctcgtgc gagatgccct
    14161 ccatcagaca gccgtcaccg acgaacaccc aggtgcggtg gtcgacgatg tcgaagccgg
    14221 gacggttgaa ctgctctgcc agcaaccgct cggcgagcgc catgccgact gcattcgcca
    14281 gaccctgtcc cagcggaccg gtggtggtct caacgccggg acagtggaga tattccgggt
    14341 ggccggcagt cttggagtcg agctgacgaa accggcgcag atcatccagc gtcacgtcat
    14401 agccactcag atgcagcaga gagtagagca gcatcgatgc gtggccattc gaaagcacga
    14461 agcggtcacg atccgcccac tgcggttggc gcggatcgtg acgcagatgg ttgcgccaca
    14521 gcacggtggc catgtcagcc atgcccatgg gcgcgccggg gtggcccgat ttggcctgct
    14581 ccaccgcatc catgctgagg aagcggatgg cattggcgag ggcatctgac cgagacataa
    14641 gacaactccg ctagtggcaa ccatcgccgc tgcgggagca gcggctgtcg aaaatggata
    14701 cgccgtgaaa acagggtcgc cattttcacc catgccatgc ggggcagcaa tgaagctggt
    14761 agctgcaggc agatagaagg aggggaggtg accgcctgtt cgagcagagc gcaagacagg
    14821 ccggatgccc tatgtgacgg ttgcgtgaaa aattaatccg cgaaacctct tgccacgact
    14881 ggattggtcc gtataatgcg ctgctctgac ggttgct
//
LOCUS       DAORCQ010000058        14863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2565_length_14863_cov_5.836980, whole genome shotgun sequence.
ACCESSION   DAORCQ010000058 DAORCQ010000000
VERSION     DAORCQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14863
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2565_length_14863_cov_5.836980"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..865)
                     /locus_tag="PKI23_07425"
     CDS             complement(2..865)
                     /locus_tag="PKI23_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNN36804.1"
                     /translation="MVASMTAYARCESNHAWGNLTWELRSVNHRYLELSLRLPEAVRE
                     IEQPLRQQLRGQLARGKVDATLSLKLDETAAHSALNSLLLEELAGLQRQLLERLPTAA
                     PLSVHQLLQWPGVICRQQVEQQELTAAALALFENTLRQLGDQRDREGSSLSQLLRQRL
                     DAIGQQVAVVQHHLPEAAAQQRARLLNRLREVSEQFDPQRLEQELLLLAQKSDVAEEL
                     DRLTLHLHETREVLRQGGAIGRRLDFIMQELNREANTLGAKSTAAPITRAVVEIKVLV
                     EQMREQVQNLE"
     gene            1005..1721
                     /gene="rph"
                     /locus_tag="PKI23_07430"
     CDS             1005..1721
                     /gene="rph"
                     /locus_tag="PKI23_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNN36805.1"
                     /translation="MRPSGRQPEDLRPIGITRHYTRHAEGSVLVEFGATRVICTASVE
                     PGVPRFLKGSGQGWITAEYGMLPRATGSRNAREAARGKQGGRTLEIQRLIGRSLRAAV
                     DLKRLGDFTITLDCDVIEADGGTRTASVTGACVALVDALRHLQRKKLLNSDPLRFLVA
                     AVSVGVWRDRALLDLDYAEDSTAETDLNVVMSEQGGLIDLQGTAEGAPFARGQLDAML
                     GLAERGIARLIERQRQALDE"
     gene            complement(1746..2522)
                     /locus_tag="PKI23_07435"
     CDS             complement(1746..2522)
                     /locus_tag="PKI23_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNN36806.1"
                     /translation="MKIISFNLDGLHNAAQRGFFDWMVAQQADVVCLQQTLSHEHELP
                     EPLRQPGGYFSYFYDAYEKEHGGVAIYCKRPPKAVMRGLGLMEADVEGRYMQVDYDQI
                     SIVSLCLPAVTLQDEHNRQLRQQMRKKLLNHLGKVRNKRREFIFCGDFQVAHLDIDSL
                     NSTEAQDNHCLSDELGEWMDDLLDGAGYVDAFRELNDEAGHYSWWPPQDKPDHLRDAW
                     RVDYQLITSTLRRKLVAAQFDYGLNFSSHAPLIVEYALPL"
     gene            2595..3263
                     /gene="pyrE"
                     /locus_tag="PKI23_07440"
     CDS             2595..3263
                     /gene="pyrE"
                     /locus_tag="PKI23_07440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010566969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNN36807.1"
                     /translation="MHDYQRDFIEFALARSALKFGTFTLKSGRSSPYFFNMGELSRGA
                     DLARLGHFYAAALLQSQLDCDLLFGPAYKGIPLAVATAIELARQHGRDLPVVFNRKEA
                     KQHGEGGLLVGAPLQGRVAIIDDVITAGTAAREVIGMIRAAGAIPAGMVVALDRMERG
                     SGPRSAIEELRQDVGIEVVSIVTLAELIRYLEEASGLRHHLPQLLAYREQYGVETGKI
                     GTMG"
     gene            3309..3986
                     /locus_tag="PKI23_07445"
     CDS             3309..3986
                     /locus_tag="PKI23_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNN36808.1"
                     /translation="MSIRLSLVPASRCCLFGLLASVLFATDLPAANEGGRLLYRYRNE
                     QGVMVLSEQIPPEQVGQEYSVLTPSGRVIKVVPKTLSKEEAERRQVELEQQKQQQEYD
                     RSLLNRYSSVRDIEAARQRKVTEIDARQALESHNRTALQRELDRQLSQAAQLELNGQP
                     VSQLARDNIDNIRLQLSEIDRKIEFLRNESQRVRSDFDRDIARFQQLTRKSGEGEHPN
                     PGTDRSP"
     gene            complement(3983..4594)
                     /gene="slmA"
                     /locus_tag="PKI23_07450"
     CDS             complement(3983..4594)
                     /gene="slmA"
                     /locus_tag="PKI23_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HNN36809.1"
                     /translation="MLDRQAREQRQSRREQILETLARMLEARPGHRITTALLAAELGI
                     SEAALYRHFPSKAKMFEALIEFVEESLFSRIQRIHNEIPAGTDRCRQLLTLLLLFCER
                     NPGITTLLNGDGLMDETHRLRLRTRQLFERLETELRRMLRDAELNEGLKTTLPQGATA
                     ALLLAQAEGQIARFVRSGFELKPTRHWEQQWQALESACFVGAS"
     gene            complement(4600..5499)
                     /gene="argB"
                     /locus_tag="PKI23_07455"
     CDS             complement(4600..5499)
                     /gene="argB"
                     /locus_tag="PKI23_07455"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNN36810.1"
                     /translation="MSITAESAHSVAQVLTEALPYIQRFSGKTMVVKYGGNAMTDDPL
                     MQGFARDIVLMKLVGMNPVVVHGGGPQINRLLDQLGIESRFIDGMRVTDSRTMEVVEM
                     VLGGSVNKEVVNTINRCGGRAIGLTGKDGQLLRARKLHATRQGSAEPVDLGQVGEVAE
                     VNTALIDMLVNSHFIPVVAPIGVGDDGTAYNINADLVAGRIAEALQAEKLILLTNVSG
                     LTDRDGKVLTGLSTAEVDALIADGTIHGGMLPKIRCALDAVKGGVRSAHIIDGRLPHA
                     VLLELLTDQGIGTLIRGAQPVAG"
     gene            complement(5534..8167)
                     /locus_tag="PKI23_07460"
     CDS             complement(5534..8167)
                     /locus_tag="PKI23_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNN36811.1"
                     /translation="MKLRRSATAPAQPSGEAAAGQTATRITPGGGGWRWGLLLWQLLA
                     VVGATLLLDHWLIEPIARQQGRLVSEQVAGRYQQAINQTLHGLQQSAQQLAQQPQLIS
                     ALQANDAAMLEQLSGQGCQLLPLCERVIVVPLGQALEQNQHQPQAAIGFALLEMIHRS
                     EAGEAVAPQLHPQPQRPQLHLVEAVVDPATRQRLGTVVVSQDPRLLNQALSRIDADLG
                     KVVLRQRIPGVTEQKLLEQGNDAGGERLTLPTSNPLWQLDFSGTPASVASAALRWLLH
                     EVMAGVALLLLLGTLLIDWLRGRQLVGDATTFERLLMERFASHAAATPRFSLPLFTTL
                     ATVMSRLFAEQQRTQPASAEPPRQSVTSEPATAVAVPADETLLSSAALQVGLLDNGDK
                     TAAVRARPAASRHPATPPARPATTPLLAAPAAEIFRAYDIRGKVGENLSADDVRLIGQ
                     AIGSEAYMRGEEKVIVGRDGRLSSPELSAALIQGLLAAGREVLDIGEVTTPMLYFATH
                     LLDSGTGVMVTGSHNPPHYNGLKVVMERQALHSADITALHQRIIEGDLMSGAGSVSQL
                     DISDSYFERIVEDIALLSDPLPVAVDCGNGIAGRYAPRLLAALGCEVTPLFCEVDGRF
                     PNHLPDPSQPRNLQALIETVQRERIPLGLAFDGDGDRLIAVTGSGKVVPADQLLMLFA
                     REVLVRNPGADVLFDIKSSRHLNRVISEAGGRPVMWKSGHSLMKAKMRETGAVLGGEL
                     SGHLFFHDRWYGFDDGLYAAARLIELLSGELEDLDHLLSLLPASVTTPELLIEVGESE
                     KFALIDRLISEGQFEDGIVTTLDGIRVDYPDGWGLVRASNTTPALTLRFEAQDNSSLQ
                     RIMALFRAQLQQLAPRLDPGF"
     gene            complement(8190..8645)
                     /gene="dut"
                     /locus_tag="PKI23_07465"
     CDS             complement(8190..8645)
                     /gene="dut"
                     /locus_tag="PKI23_07465"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNN36812.1"
                     /translation="MKPVQIRVLDPRLGSEIPLPAHATSGSAGMDLRALLDAPLTLLP
                     GASELIPTGLAIHLADPQLAALILPRSGLGHRHGIVLGNLTGLIDSDYQGELLISCWN
                     RSAQPFVISPGERIAQLVIVPVVQTQWELVSEFPPTARSEGGFGHTGQQ"
     gene            complement(8642..9853)
                     /gene="coaBC"
                     /locus_tag="PKI23_07470"
     CDS             complement(8642..9853)
                     /gene="coaBC"
                     /locus_tag="PKI23_07470"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNN36813.1"
                     /translation="MQRLRGKQILLGITGGIAAYKSAELTRGLKKAGAQVRVVMTAAA
                     REFITPLTLQALSGHPVHTDLLDPAAEAAMGHIELARWADLVLIAPASADFLARLAQG
                     QGDDLLATLCLATRAPIAVAPAMNQAMWQAASTQHNIALLRGRNVAIWGPAEGEQACG
                     ESGPGRMIEVDELLRSAAESFESGLLTGLKVVVTAGPTREAIDPVRYISNHSSGKMGY
                     ALASAAAEAGAEVVLISGPTMLATPEGVRRVDVTTACQMLDATLAELDGCALFIATAA
                     VADYRPQQAAMQKIKKRQGEESLTLTLVRNPDIVATVARQTPRPFTVGFAAETEQLLA
                     HARAKLIDKKLDLVIANDVSDSRIGFNSDQNQVTLVSAAGELELPCLAKGRLAHELIG
                     HIHRLYQTRPQ"
     gene            9927..10871
                     /locus_tag="PKI23_07475"
     CDS             9927..10871
                     /locus_tag="PKI23_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNN36814.1"
                     /translation="MTGFYHGMMPQSNPKNGDDRSRNHRFRAGSSPWHRDAPAAARAA
                     MMRLRQRVKWLLLPPGLNLLLVSIGLLLWPGWPIAGLLLVALSLLLLLLFSSGSIVRR
                     WQRRWSLPTRLAGAGAARPPAAIVILGAGRRHRRSGDCLTRLGIERLHRGAALARSSG
                     LPLLLSGGRAGAASSQPSEAALMQDYLVQQLGLTPQWLEQQSRTTWENARMSTPLLAD
                     QQIDAIYLVSHGWHLRRACLAFAQDGMMIWPVAVEPRAVEVQALRSGWPSRTALRLSL
                     YLLHELFGYLWYRRLARRYSAAASVARPTPNSGQSPKR"
     gene            complement(10778..12298)
                     /gene="lnt"
                     /locus_tag="PKI23_07480"
     CDS             complement(10778..12298)
                     /gene="lnt"
                     /locus_tag="PKI23_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNN36815.1"
                     /translation="MIRALNPDRQPLRGDALALIAGLLLPLALAPLAWRPLALLSPAL
                     WLWTLLDATPRRAAWRGLLFGLGSFGLGVSWVFVSIHLYGHAPVVLAALLTLLFVAAM
                     ALYPTLLAWLLAHLSRHVPGAALTLFPLLWVAIEWLRGWLLTGFPWLQLGTAVLDTPW
                     AGWLPLLGVLGGSYLLALLAAVAVQALRGSLHWGWPLLGLLLISGAGLWSQQQAWTRP
                     LPQSRVTVGLVQPNIPQDLKWTPEQRQATLERLDQLSAPLWGVDLLLWSEAAVPLFFH
                     QAQDYLAQAGQRAEQSGSALLLGIPFMTMAAQGSGFVAHNSLIALGNGEGLYHKQRLV
                     PFGEYVPLERWLRGLIAFFDLPMSDFTAGAAGQPPISTTQGWRILPLICYEVAYADLA
                     RAQPADLLLTVSNDAWFGDSFGPWQHLEIARLRAAENGRPLLRATNDGISALIDHQGA
                     LLLQSERFVAQSLHGEVVLTAGETPYHRFGDWPLFGVGLATLAAALYRRARRRYQR"
     gene            complement(12295..13137)
                     /locus_tag="PKI23_07485"
     CDS             complement(12295..13137)
                     /locus_tag="PKI23_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003301723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNN36816.1"
                     /translation="MNRSAKEAAEPLLPRLLRLLGYTPPGRTTLLEHLHAAEQRNELD
                     ADAVGIIEGALQVAEMQVRDIMIPRAQVVSIDADEQLEQFLPKVIESAHSRFPVIDSP
                     DEVIGILLAKDLLPLLLSGSGRGYFSIKEYLRPPVFVPESKRLNVLLREFRANRNHMA
                     VVVDEYGGMAGVVTIEDVLEQIVGEIEDEHDIDDENFIKQYDQQTFVVKAITPVSDFN
                     ARFATRFDEDEFDTIGGIVIQAFGRLPKRNESITIDELTFKVLNADHRRIRLLRVHRT
                     AGSE"
     gene            complement(13134..13604)
                     /gene="ybeY"
                     /locus_tag="PKI23_07490"
     CDS             complement(13134..13604)
                     /gene="ybeY"
                     /locus_tag="PKI23_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNN36817.1"
                     /translation="MKLQLDLQRATTLTPPSRPTLRRWLLRALTPHRDRAQLTLRLVD
                     EAESAELNQQYRGKPGPTNVLAFPSPLPEGVQADFLGDLVICAPVVEREAREQGKSPR
                     AHWAHIVIHGALHLCGHDHQNDAEAQRMEQLEIELLRSLRIANPYQSRHIESTP"
     gene            complement(13601..14611)
                     /locus_tag="PKI23_07495"
     CDS             complement(13601..14611)
                     /locus_tag="PKI23_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNN36818.1"
                     /translation="MNSQLEIRQLLLEPDNARRLASLCGQLDANLRHIEQRLDIEIHN
                     RGNAFKLLGSSQSVSTAQRLLQQLYAETARGTEMTPDLLHLHLQESSLEALNQSAVEA
                     VAPRNLLIRTPRLAVKPRGSNQQLYVRAIDEHDINFGLGPAGTGKTFLAVACAVRALE
                     EERVGRILLVRPAVEAGEKLGFLPGDLASKIDPYLRPLYDALYEMMGFERVGKLIERN
                     VIEVAPLAFMRGRTLNNAFIILDEGQNTTGEQMKMFLTRIGFGSTVVVTGDLTQTDLP
                     RGVRSGLWQAVQILQGIAGVSFTRFDSKDVVRHPLVQKIVEAYDRLQTDEDEGSRRSV
                     TG"
     gene            complement(14721..>14863)
                     /locus_tag="PKI23_07500"
     CDS             complement(14721..>14863)
                     /locus_tag="PKI23_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNN36819.1"
                     /translation="GEGELCGRTGNNRVVNFPASDVGLIGRMANVEIVEVLPNSLRGQ
                     LL"
BASE COUNT         2434 a         4879 c         4993 g         2557 t
ORIGIN      
        1 gctactccag attctgcacc tgctcgcgca tctgctccac cagcaccttg atctcgacca
       61 ccgcacgggt gatcggcgcg gcggtcgatt tggcgcccag ggtgttggcc tcgcggttga
      121 gctcctgcat gatgaagtcg agccggcggc cgatggcgcc cccctggcgc agcacctcgc
      181 gggtttcatg caggtgcagg gtcagccggt cgagctcttc ggcgacgtcg gacttctgcg
      241 ccagcagcag cagctcctgc tccagccgct gcggatcgaa ctgttcactc acttcacgca
      301 atcggttgag cagccgggcg cgctgctgcg cggcggcctc cggcagatgg tgttggacca
      361 cggcgacctg ctgcccgatg gcgtcgagcc gctggcgcag cagttggctc aagctgctgc
      421 cttcgcggtc acgctggtcg cccagttgcc gcagtgtgtt ttcgaacagc gccagcgcgg
      481 cggcagtcaa ctcctgctgc tcgacctgct gccggcagat gacgccgggc cactgcagca
      541 gttgatgcac gctcagtggc gcagcggtgg ggagccgctc cagcaactgc cgctgcaagc
      601 cagccaactc ttcgagcaac aggctgttga gcgcactgtg cgcggccgtc tcatcgagct
      661 tgagcgagag ggtggcgtcg accttgccgc gcgccagttg accgcgcagt tgctgccgca
      721 gtggctgctc gatctcccgc actgcttcgg gcaggcgcag gctcagctcc agatagcggt
      781 ggttcaccga gcgcagttcc caggtgaggt tgccccaggc gtggttcgat tcgcagcggg
      841 cataggccgt catgctggca accatcggtt cggctccgtc gggttgttga aagagggtgg
      901 ggccgcgact ctacggcagc ctccggtggg gaacaagtcc gggcggcctt gggcttgcgt
      961 tcggctataa tggccgccct tttcagcaac gatggcggta cacgatgcgt cccagtggtc
     1021 gacagcccga agatttgcgc ccgatcggca tcacccgcca ctacacccgc catgccgaag
     1081 ggtcggtgct ggtggagttc ggcgccaccc gcgtcatctg caccgcctcg gtcgagccgg
     1141 gtgtgccccg gtttttgaag ggcagcggac agggctggat caccgccgag tatggcatgt
     1201 tgccgcgcgc caccggcagc cgcaacgcgc gcgaagccgc ccgcggcaag cagggcggac
     1261 ggacgctgga gatccagcgg ctgatcggcc gctcgctgcg cgcggcggtc gatctgaagc
     1321 ggcttggcga cttcaccatc acgctcgatt gcgatgtgat cgaggccgat ggcggcaccc
     1381 gcaccgcctc ggtgaccggc gcctgcgtgg cgctggtcga tgcattgcgt catctgcaac
     1441 gcaagaagct gctgaacagc gatccgctgc gctttctggt ggcggcggtg tcggtcgggg
     1501 tgtggcgcga ccgggcgctg ctcgatctgg actacgccga ggactcgacc gccgagaccg
     1561 atctgaacgt ggtgatgtcg gagcagggcg gactgatcga cctgcagggc acggccgagg
     1621 gggcgccctt cgcccgtggg cagctcgatg cgatgctggg tctggccgag cgcggcattg
     1681 cccggttgat cgaacggcag cggcaggcgc tggacgagtg agccgtcggg cgccgccggc
     1741 acccttcaca gcggcagcgc gtactcgacg atcagcgggg cgtggctgga gaagttcagg
     1801 ccgtagtcga actgcgcggc aaccagcttg cgccgcaggg tcgaggtgat gagctggtag
     1861 tcgacccgcc aggcatcgcg caggtgatcg ggtttgtcct ggggcggcca ccagctgtag
     1921 tggccggctt cgtcattcag ctcgcggaag gcatcgacgt agccagcgcc atcgagcagg
     1981 tcatccatcc actcacccag ctcgtcggac aggcagtggt tgtcctgggc ctcggtcgag
     2041 ttgagcgagt cgatgtcaag gtgggccacc tgaaaatcgc cgcagaagat gaactcccgc
     2101 cgcttgttgc gcaccttgcc cagatggttg agcagcttct tgcgcatctg ctgccgcaac
     2161 tgccgattgt gctcatcctg cagcgtgacc gccggcaggc agagcgagac gatgctgatc
     2221 tggtcatagt cgacctgcat gtagcgccct tcgacatcgg cctccatcag gccgagcccg
     2281 cgcatcaccg ccttgggcgg ccgcttgcag tagatggcga cgccgccgtg ctctttttca
     2341 taggcatcgt aaaagtaact gaagtaaccg ccgggttgcc gcaacggctc gggcagttca
     2401 tgttcgtggc tgagcgtctg ctgcaggcag accacatcgg cctgctgcgc gaccatccag
     2461 tcaaagaaac cgcgctgggc ggcgttgtgc aggccgtcga gattgaagct gattattttc
     2521 atgatgggtc gcgatccttg gaaccggtat cataccggag cagcactgct ccattaaacg
     2581 ttgtgacctg atccatgcac gattatcaac gcgacttcat cgaattcgcc ctggcgcgct
     2641 cggcgctcaa gttcggcacc ttcacgctga agtcgggacg cagcagcccc tacttcttca
     2701 acatgggtga actctcgcgc ggcgccgacc tggcgcggct gggccacttc tatgccgcgg
     2761 ccctgctgca gagccagctc gattgtgacc tgctgttcgg accggcctac aagggcatcc
     2821 cgctggcagt ggccaccgcc atcgagctgg cgcggcaaca tggccgcgac ctgccggtgg
     2881 tgttcaaccg caaggaggcc aagcagcatg gcgaaggggg actgctggtc ggcgcgccgc
     2941 tgcaggggcg ggtggcgatc atcgacgacg tgatcaccgc cggcaccgcg gcccgtgagg
     3001 tgatcgggat gattcgcgcc gccggcgcca tcccggccgg catggtggtg gcgctcgacc
     3061 gcatggagcg cggcagcgga ccgcgctcgg cgatcgagga gctgcgccag gatgtcggca
     3121 tcgaagtggt gagcatcgtc acgctggcag agctgatccg ctacctcgaa gaggcgtccg
     3181 gcctgcgcca tcatctgccg caactgctcg cctaccgtga gcagtacggg gtggagacag
     3241 gcaaaatcgg cacaatggga tagacttcaa agcatatcca tccctattca cccaggttcc
     3301 ggttgtcgtt gtccatcaga ctttcccttg tccccgcatc gcgctgctgc ctgttcggcc
     3361 tgctggcgtc ggttctcttc gccaccgatt tgccggctgc caacgagggc ggccggctgc
     3421 tctatcgcta ccggaacgag cagggggtga tggtgctgag cgagcagatt ccacccgaac
     3481 aggtggggca ggagtacagc gtgctgacgc cgagtggccg ggtgatcaag gtggtgccca
     3541 agacgctgtc gaaagaggag gccgaacgtc gtcaggtgga gctggagcag cagaaacagc
     3601 agcaggagta tgaccgctcg ctgctcaacc gctacagctc ggtccgtgac atcgaggccg
     3661 cgcggcagcg caaggtgacc gagatcgacg cccgccaggc actggagtcg cacaaccgca
     3721 ccgcattgca gcgtgagctg gaccggcaac tgtcacaggc ggcccagctc gagctgaatg
     3781 gccagccggt gtcgcaactg gcgcgggaca acatcgacaa catccgcctg caactgagtg
     3841 agattgaccg caagatcgag ttcctgcgca acgagagcca gcgcgtgcgc agcgacttcg
     3901 accgcgatat cgcccgcttt cagcaactca cccgcaagag cggtgagggt gaacatccca
     3961 accccgggac ggatcggtcg ccctagctcg caccgacgaa acaggctgac tccaatgcct
     4021 gccactgctg ctcccagtgg cgggtcggct tcagttcaaa gccgctgcgc acgaaacggg
     4081 cgatctgccc ttcggcctgc gccagcagca gggcggcagt ggccccctgc ggcagtgtgg
     4141 ttttcagccc ctcgttcagt tcggcatcac gcagcatcct gcgcagctcc gtctccagcc
     4201 gttcgaacag ctgtcgcgtg cgcagccgca gccggtgggt ctcatccatc aatccgtcgc
     4261 cattgagcag cgtggtgatg ccggggttgc gctcacagaa cagcagcagc agggtcagca
     4321 gctgccggca gcggtcggtg ccggccggaa tctcgttgtg gatgcgctga atgcgggaga
     4381 agaggctctc ttcgacgaat tcgatcagcg cctcgaacat cttcgccttg gagggaaagt
     4441 ggcggtagag cgccgcctcg gagattccca gctcggcggc cagcagtgcg gtggtgatcc
     4501 ggtggccggg ccgtgcctcc agcatccttg ccagggtctc cagaatctgc tcgcggcgcg
     4561 actgccgctg ttcgcgggcc tggcgatcga gcatgccgtt cagcccgcca ccggttgcgc
     4621 accgcggatc agggtgccga tgccctggtc ggtcagcagc tccagcagca ccgcgtgtgg
     4681 cagccggcca tcgatgatgt gggccgagcg gacgccgccc ttcaccgcat cgagggcgca
     4741 gcgaatcttc ggcaacatgc cgccatggat ggtgccatcg gcgatcagcg catcgacctc
     4801 tgcggtcgag agtccggtca gcaccttgcc atcacggtcg gtcagcccgc tgacattggt
     4861 cagcagaatc agcttttcgg cctgcaacgc ctcggcgatc cggccggcca ccagatcggc
     4921 gttgatgttg taggcggtgc catcgtcacc caccccgatc ggcgcgacca ccggaatgaa
     4981 gtggctgttg accagcatgt cgatcagcgc ggtgttgacc tcggccacct cgccgacctg
     5041 gcccagatcg accggttcgg cgctgccctg gcgggtggcg tgcagcttgc gcgcccgcag
     5101 cagctggcca tccttgccgg tgagtccgat cgcccgaccg ccacagcggt tgatggtgtt
     5161 gaccacctcc ttgttgacgc tgccgcccag caccatctcc accacctcca tggtgcggct
     5221 gtcggtcacc cgcatgccgt cgatgaagcg tgattcgatg ccgagctgat cgagcagccg
     5281 gttgatctgc gggccgccgc catgcaccac caccgggttc atgccgacca gtttcatcag
     5341 cacgatgtcg cgggcgaagc cctgcatcag cgggtcatcg gtcatcgcat tgccaccgta
     5401 cttgaccacc atggtcttgc cggagaagcg ttggatgtag ggcagcgcct cggtcaatac
     5461 ctgggcgaca ctgtgggcag attcagcagt gatggacatg gcaatggtct tggaagttga
     5521 gggtcgggcg tggtcagaaa ccggggtcga gtcggggggc gagctgttgc aactgggcgc
     5581 ggaacagcgc catgatccgt tgcaggctgc tgttgtcctg cgcctcgaag cgcagcgtca
     5641 gcgccggggt ggtgttggag gcgcgcacca gcccccagcc atccggatag tcgacccgta
     5701 tgccatcgag ggtggtgacg atgccatcct cgaactgccc ttcactgatc agacgatcga
     5761 tcagcgcgaa cttctccgac tcgcccactt cgatcagcag ctccggggtg gtcacgctgg
     5821 ccggcagcag cgacagcaga tggtcgagat cctccagctc accgctcagc agttcgatca
     5881 gccgggcagc ggcgtagagg ccatcatcga agccatacca gcggtcatgg aagaagaggt
     5941 ggccgctcag ctcgccgccc agcaccgcgc cggtttcgcg catcttcgcc ttcatcagcg
     6001 aatggccgct cttccacatc accggccggc cgccggcctc gctgatcacg cggttgaggt
     6061 ggcggctcga cttgatgtcg aacagcacat cggcgccggg attgcgcacc agcacctcgc
     6121 gggcgaacag catcagcaac tggtcggcgg ggaccacctt gccgctgccg gtcacggcga
     6181 tcaaccggtc gccatcgccg tcgaaggcga gccccagcgg aattcgctca cgctgcacgg
     6241 tctcgatcag cgcctgaaga ttcctgggct gactggggtc gggcaggtgg ttggggaagc
     6301 ggccatccac ctcgcagaac agcggcgtca cctcgcagcc gagcgccgcc agcagccggg
     6361 gcgcgtagcg gccggcgatg ccgttgccgc aatcgaccgc caccggcagc ggatcggaga
     6421 gcagcgcgat gtcctcgacg atccgctcga agtagctgtc gctgatgtcg agctgtgaga
     6481 cgctgccggc cccgctcatc aggtcgcctt cgatgatgcg ctggtgcagc gcggtgatgt
     6541 cggcgctatg cagcgcctgg cgctccatca ccaccttgag gccgttgtag tggggcggat
     6601 tgtggctgcc ggtcaccatc acgccggtgc cgctgtcgag cagatgggtg gcgaagtaga
     6661 gcatcggcgt cgtcacctcg ccgatgtcga gcacctctct tccggcggcg agcagcccct
     6721 ggatcagcgc cgcgctcagt tctggactgc tgagccgtcc gtcacggccg acgatcacct
     6781 tctcctcgcc gcgcatgtag gcttcactgc cgatcgcctg accgatcagg cgcacatcgt
     6841 cggccgagag gttttcgccc accttgccgc ggatgtcgta ggcgcgaaag atctccgctg
     6901 ccggtgcggc cagcagcggt gtggtggcgg gtctggcggg cggcgttgcc ggatgacgcg
     6961 atgcagcggg tctcgccctg actgcagcgg ttttgtcacc gttgtcgagc agaccgacct
     7021 gcagcgcggc gctggagagc agcgtctcgt ccgctggtac tgcgaccgcc gtggctggtt
     7081 cggacgtgac cgattgccgg ggcggttcgg cgctggccgg ttgtgtgcgc tgctgttcgg
     7141 caaacagccg gctcatcacg gtggccagcg tggtgaacag cggcagactg aagcggggcg
     7201 tggccgcggc atggctggcg aaacgctcca tcagcagccg ctcgaaggtg gtggcatcgc
     7261 cgacgagctg gcggccgcgc agccagtcga tcagcagggt gcccagcagc agcagcagcg
     7321 ccacgccggc catcacttca tggagcagcc agcgcagcgc agcggaagcg acgctggccg
     7381 gagtgccgga aaaatcgagc tgccacaagg ggttgctggt ggggagggtg agccgctcac
     7441 cgccggcatc gttgccctgc tccagcagct tctgctcggt gacaccggga atgcgctggc
     7501 gcagcaccac cttgcccaga tcggcatcga tgcggctgag cgcctggttc agcaaccgtg
     7561 gatcctggct gaccaccaca gtgcccaagc gttgccgggt ggcgggatcg accaccgcct
     7621 cgaccagatg caactgcggc cgctgcggtt gcggatgcag ctgcggggcc acggcttcgc
     7681 ccgcttcact gcggtggatc atctccagca gcgcgaagcc gatcgccgcc tgtggctgat
     7741 gttgattctg ctcgagggcc tgccccagcg gcaccacgat gacgcgctcg cacagcggca
     7801 gcagttggca gccttgcccg ctcaactgct ccagcatcgc tgcatcattt gcctgcagcg
     7861 cactgatcaa ttgcggttgc tgggccagtt gctgtgcgga ttgctgcagg ccatgcagcg
     7921 tctgattgat cgcctgctga taacgcccgg ccacctgctc gctgaccagc cgcccctgct
     7981 gtcgggcaat cggctcgatc agccagtgat cgagcagcag agtggcgccg accactgcca
     8041 gcagttgcca cagcagcagg ccccagcgcc agccaccgcc acccggcgtg atgcgggtgg
     8101 cggtctgccc ggccgccgcc tcgccggagg gctgcgcggg tgccgttgcg cttcgccgta
     8161 acttcatgct ggataccctg aggagaagat cactgctgtc cggtgtgacc aaaaccaccc
     8221 tcggaccggg ccgtgggtgg gaattcgctg accagctccc actgcgtctg caccactggc
     8281 acgatcacca gttgcgcgat ccgctcgccg gggctgatca caaagggctg cgccgagcgg
     8341 ttccagcagg agatcagcag ctccccctga tagtcggagt cgatcagccc ggtgaggttg
     8401 ccgagcacga tgccatgtcg gtggccgagg ccggagcgcg gcaggatcag cgcggccagc
     8461 tgcggatcgg ccagatggat cgccaaccct gtgggaatca gctcgctggc accgggcagc
     8521 agcgtcagcg gcgcatcgag cagggcacgc agatccatgc cggccgaacc gctggtggcg
     8581 tgtgccggca gcggaatctc gctgccgagg cgtgggtcga gcaccctgat ctgtaccggt
     8641 ttcactgtgg acgtgtctga tagagccggt ggatgtggcc gatcaattca tgggcgagcc
     8701 gccccttggc aaggcagggc aactccagtt ctcccgctgc cgagaccagc gtcacctgat
     8761 tctggtcgct gttgaagccg atgcggctgt cggagacatc gttggcgatc accagatcga
     8821 gcttcttgtc gatcagttta gcccgtgcat gggccagcag ctgctcggtc tcggcggcaa
     8881 agccgaccgt gaagggtcgt ggcgtctggc gggcgaccgt ggcgacgatg tcggggttgc
     8941 gcaccagcgt cagggtcaga ctctcctcac cctggcgctt cttgatcttt tgcatggccg
     9001 cctgctgcgg ccggtagtcc gccaccgcgg cggtggcgat gaacagcgcg cagccgtcga
     9061 gctcggccag cgtggcatcg agcatctggc aggcggtggt gacatcgaca cggcgcaccc
     9121 cttccggggt cgccagcatg gtcgggccgc tgatcagcac cacctcggca ccggcctcgg
     9181 cggcggcgct ggccagcgca tagcccatct tgcccgagct gtggttgctg atgtaacgca
     9241 ccgggtcgat cgcctcgcgg gttggcccgg ccgtcaccac caccttcagg ccggtcagca
     9301 ggccactttc aaacgactcc gccgccgacc gcagcagctc atccacttcg atcatgcggc
     9361 ccggaccgct ctcgccgcag gcctgctccc cttcggccgg tccccagatg gcgacgttgc
     9421 gaccacgcaa cagggcgatg ttgtgctggg ttgatgccgc ctgccacatc gcctggttca
     9481 tggcgggcgc caccgcaatg ggggcccggg tggccagaca gagtgtggcc agcaggtcat
     9541 caccctgacc ctgcgccagt cgcgccagaa aatcggccga cgccggcgcg atcagcacca
     9601 gatcggccca gcgtgccagc tcgatgtggc ccatcgccgc ttcggccgcc gggtcgagca
     9661 ggtcggtgtg aaccggatgg cccgagagcg cctgcagcgt gagcggggtg atgaattcac
     9721 gggcggccgc ggtcatcacc acccgcacct gcgcgccagc cttcttcaat ccgcgggtca
     9781 gttcggcgct cttgtaggcg gcgatgccac cggtgatgcc gagcaggatc tgcttgccgc
     9841 gcagccgttg catgtatgtt ccctgaccct cgctgaagat gggtgaccgc ttcgccaacc
     9901 gcaaaacgcg gcggtaagat accaccgtga ccggttttta tcatggcatg atgccgcaat
     9961 ccaacccgaa gaacggcgat gaccgctccc gcaaccatcg gttccgcgcc ggcagcagcc
    10021 cttggcatcg cgacgcccca gctgcggcgc gcgctgccat gatgcggctg cggcagcggg
    10081 tgaagtggct gctgctgcca ccgggcctca atctgctgct ggtgtcgatc ggtctgctgc
    10141 tctggccagg ctggccgatt gccggccttc tgctggtcgc gctttcgctg ctgttgttgc
    10201 tgctgttcag ttcgggctcc atcgtgcggc gctggcagcg gcgctggtcg ctgccgaccc
    10261 gtctggccgg tgccggggcg gcgcggccgc cagccgccat cgtcattctc ggcgcgggcc
    10321 gccgtcatcg gcgcagtggt gactgcctga cccggctcgg catcgagcgg ttgcaccgtg
    10381 gtgcggcgct ggcgcgcagc agtggcctgc cgctgctgct gagcggtggc cgggccgggg
    10441 cagcgtcctc gcagccgagt gaagcggccc tgatgcagga ctatctcgtc cagcagctcg
    10501 gcctcacgcc gcagtggctg gagcagcagt cacgcaccac ctgggagaat gcccggatga
    10561 gcacgccgct gctggctgac cagcagatcg acgcgatcta tctggtcagc catggctggc
    10621 acctgcggcg cgcctgcctg gccttcgcgc aggacggcat gatgatctgg ccggtggcgg
    10681 tcgagccgcg cgccgtcgag gttcaggcgc tgcgcagtgg ctggccgagc cggaccgccc
    10741 tgcggctcag cctctatctg ctgcacgaac tgttcggcta tctctggtac cgccgcctcg
    10801 cgcgccggta cagcgccgcc gccagcgtgg ccaggccgac accgaacagc ggccagtcgc
    10861 cgaagcggtg atagggggtc tctccggcgg tcagtacgac ctcgccatgc aggctctgcg
    10921 cgacgaagcg ctcactttgc agcagcagcg ccccctgatg atcgatcagc gccgagatgc
    10981 catcgttggt ggcgcgcagc aacggtcggc cattctcggc ggcacgcagc cgggcgatct
    11041 ccagatgttg ccaggggccg aagctgtcgc cgaaccaggc atcgttgctg accgtcagca
    11101 gaaggtccgc cggctgagcc cgggccagat cggcataggc cacttcgtag cagatcagcg
    11161 gcaggatgcg ccagccctgg gtggtgctga tcggcggttg accggccgct cccgctgtga
    11221 agtcggacat cggcagatcg aagaaggcga tcagcccgcg cagccagcgc tccagcggga
    11281 catactcgcc aaagggcacc agccgctgct tgtggtagag cccctcgccg ttgccgagcg
    11341 caatcaggct gttgtgggca acgaagccgc tgccctgcgc tgccatcgtc atgaacggga
    11401 tgcccagcag cagcgcgctg ccgctctgct cggcgcgctg acccgcctgc gccagataat
    11461 cctgcgcctg atgaaagaac agcggtacgg ctgcctcgga ccagagcagc aggtcgacgc
    11521 cccacagcgg cgcgctgagc tgatcgagcc gctccagcgt cgcctgccgc tgctcggggg
    11581 tccatttgag atcctgcgga atgttcggct gcaccagccc gacggtgacg cggctctgtg
    11641 gcaatggtcg ggtccacgcc tgctgttgcg accagagtcc tgcgccactg atcagcagca
    11701 gtcccagcag tggccagccc cagtgcaggc tgccgcgcag cgcctgcact gcaaccgctg
    11761 ccagcagtgc cagcagatag ctgccaccca gcacccccag cagcggcagc cagccggccc
    11821 atggcgtatc cagcacggcc gtgccgagct gcaaccaggg gaacccggtt aacagccagc
    11881 cgcgcagcca ctcgatggcc acccacagca gcggaaagag ggtcaacgca gcacctggaa
    11941 catggcggct cagatgggcc agcagccagg ccagcagcgt cggatagagc gccatggccg
    12001 ccacgaacag cagcgtcagc agcgccgcca gcaccaccgg cgcatggcca tagaggtgga
    12061 tgctgacgaa gacccaggag acgccaagac caaagctgcc aaggccgaac agcaggccac
    12121 gccaggcggc gcggcgcggc gtggcgtcca gcagggtcca cagccacaat gccggactga
    12181 gcagcgccag tggccgccac gccagtggcg ccaatgccag cggcagcagc aggccggcga
    12241 tcagcgccag cgcatcaccc cgcagcggct ggcggtcggg attgagtgcg cgaatcactc
    12301 gctgcccgcg gtacggtgca cccggagcag gcggatacgg cggtggtcgg cgttgagcac
    12361 cttgaaggtg agctcatcga tggtgatgct ctcgttgcgc ttcggcagcc ggccgaaggc
    12421 ctgaatgacg atgccgccga tggtgtcgaa ctcatcctcg tcgaagcggg tggcgaagcg
    12481 ggcattgaag tcgctgaccg gggtgatcgc cttgaccacg aaggtctgct ggtcgtactg
    12541 cttgatgaag ttctcgtcgt cgatgtcatg ctcatcctcg atctcgccga cgatctgctc
    12601 cagcacatcc tcgatggtga cgacgcccgc catgccgcca tactcatcca ccaccaccgc
    12661 catgtggttg cggttggcgc ggaactcccg cagcagcacg ttgagccgct tgctctcggg
    12721 cacgaagacc ggcgggcgca gatactcctt gatcgagaag taaccccggc cgctgccgct
    12781 cagcagcagc ggcagcagat ccttggccag caggatgccg atcacctcgt ccggtgagtc
    12841 gatcaccgga aaccgggagt gggccgattc gatcaccttg ggcagaaact gctccagctg
    12901 ctcgtcggca tcgatgctga ccacctgtgc acgtggaatc atgatgtcgc gcacctgcat
    12961 ctcggcgacc tgcaacgccc cttcgatgat gccgacggca tcggcatcca gctcgttgcg
    13021 ctgctcggcg gcatgcagat gttcgagcag ggtggtgcgc ccgggcgggg tgtagcccag
    13081 cagcctgagc agacgcggca gcagtggttc ggcggcctcc ttggcggagc ggttcatggt
    13141 gtcgactcga tgtggcggct ttgatagggg ttggcgatgc gcaggctgcg gagcagttcg
    13201 atctcgagct gctccatccg ctgggcttcg gcgtcattct gatggtcatg gccacacagg
    13261 tgcagtgcgc catggatgac gatgtgcgcc cagtgtgccc gcggtgactt gccctgctcg
    13321 cgcgcctcgc gctccaccac cggcgcgcag atgaccagat caccgagaaa gtcggcctgc
    13381 accccctccg gcagcggact ggggaaggcg agcacattgg tcgggcccgg cttgccgcgg
    13441 tactgctggt tcagttcggc gctctcggcc tcgtcgacca gccgcagcgt cagctgcgca
    13501 cggtcgcggt ggggtgtcag ggcgcgcagc agccagcggc gcagggtcgg gcggctgggc
    13561 ggggtcagcg tggtggcccg ttgcaggtcg agctgaagtt tcatccggtg accgagcggc
    13621 gactgccctc atcctcatcg gtctgcaggc ggtcgtaggc ttcgacgatc ttctgcacca
    13681 gtggatggcg caccacatcc tttgagtcga accgggtgaa actgacgccg gcaatgccct
    13741 gcagaatctg caccgcctgc cacagtccgg agcggacacc gcgcggcagg tcggtctggg
    13801 tcaggtcacc ggtgaccacc acggtggagc cgaaaccgat gcgggtcagg aacatcttca
    13861 tctgctcgcc ggtggtgttc tgcccctcgt cgaggatgat gaaggcgttg ttcagtgtgc
    13921 ggccacgcat gaaggccagc ggcgccacct cgatgacatt gcgctcgatc agcttgccga
    13981 cccgctcgaa acccatcatt tcatagaggg cgtcatagag cggccgcagg taggggtcga
    14041 tcttgctggc cagatcgccg ggcagaaagc cgagcttttc accggcctcg accgccgggc
    14101 gcaccagcag gatgcggccg acccgctcct cctccaaggc cctgaccgcg caggcgacgg
    14161 cgagaaaggt cttgccggtg ccggcaggac ccaggccgaa gttgatgtca tgctcatcaa
    14221 tggcgcggac atagagctgc tggttgctgc cacgcggctt gaccgccagg cgcggcgtgc
    14281 ggatcagcag gtttcttggc gcaacggcct cgacggcgct ctggttgagt gcttccaagc
    14341 tggactcctg caggtgaagg tggagcagat cgggggtcat ttcggtgccg cgggcggtct
    14401 ctgcgtagag ttgctgcaac agccgctgcg cggtcgacac cgactgggag ctgcccagca
    14461 gcttgaacgc gttgccgcgg ttgtggattt cgatgtccag tcgctgttcg atgtggcgaa
    14521 ggttggcgtc gagctgaccg cacaggctcg ccagccggcg ggcgttgtcg ggctcaagca
    14581 gaagctggcg gatttccagt tggctgttca agatggcggt gggcaacccc gtgactcagg
    14641 cggatggctt caagcatata cgctgcacgg tgaccgagaa aagtcctttt tgatgacaat
    14701 gtcatggcga tgcggcccgt tcagagcagc tgcccgcgca gcgaattggg cagcacctcg
    14761 acgatctcca cgttggccat ccggccgatc agaccgacat cgctggccgg gaagttgacc
    14821 acccggttgt tgccggtgcg gccgcagagc tctccctccc ccc
//
LOCUS       DAORCQ010000059        14733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2609_length_14733_cov_6.084003, whole genome shotgun sequence.
ACCESSION   DAORCQ010000059 DAORCQ010000000
VERSION     DAORCQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_14733_cov_6.084003"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PKI23_07505"
     CDS             <1..1122
                     /locus_tag="PKI23_07505"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNN36820.1"
                     /translation="MKSVRPPVLWTNLLLFVIPSTLALVAVPLYGWWHGYDGFEVGLF
                     LFFGAASGTAITAGFHRLWSHQAYEAHPLLRLWFALWGAAALQNSILNWASDHRRHHR
                     HVDDPQLDPYSARRGFWFAHIGWMLREYRAQQDDFSNVKDLQKDPIVMWQHRNYLLLV
                     LLMNVALPALIGVVHGDLLGSLLLAGVLRLVVVHHFTFFINSLAHFWGRQPYSSDCTA
                     RDNAVVALLTFGEGYHNFHHQFQWDYRNGIRWWQFDPTKWLIRVCAWLGLSRNLKRCS
                     PEKIEQAILQQQFRRSSQQISRLPDADSHLRRLQQEYDRLIEMVQAWGESRQRWADAR
                     ANQLLQQWERAQIRRSCLELRQRLLEQRRDWELLLARLT"
     gene            complement(1182..1979)
                     /locus_tag="PKI23_07510"
     CDS             complement(1182..1979)
                     /locus_tag="PKI23_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNN36821.1"
                     /translation="MGFLTGKRALIVGVASKLSIAYGIAEAMHREGAELAFTYQGEKL
                     KERVEEFAKGWDSTLCYPCDVASDDEIDAVFTELGKVWDGLDILVHCVGFAPREELDG
                     DYLAVTSRDGFRIAHDISAYSFVALAKAARPMLVKSHGSLLTLTYLGSQRTMPNYNVM
                     GLAKASLEASVRYLANSLGPQGVRVNAISAGPIRTLAASGIKDFRKMLSYNEARTPLR
                     RNVTIHEVGNTAAFLCSELASGITGEVVYVDGGFNITAMGDIDEASS"
     gene            2102..2449
                     /locus_tag="PKI23_07515"
     CDS             2102..2449
                     /locus_tag="PKI23_07515"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNN36822.1"
                     /translation="MNDLTQQQCEACRIGAPKVTEAERAELLPKIPDWKIERDGHLDK
                     LKREYRFDDYPKALAFTQQIGELAEAQGHHPAILTEWGKVTVWWWSHKIEGLHRNDFI
                     MAAKSDLCYATKG"
     gene            2489..3301
                     /locus_tag="PKI23_07520"
     CDS             2489..3301
                     /locus_tag="PKI23_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNN36823.1"
                     /translation="MIDLTVAERFIEAGGHRLKCTTLGQPDPARPTLVFLHEGLGSIS
                     MWKDYPAQVAEQVGLPVLMYDRWGHGESDPLTEPRKPSYRRVEALEVLPEVLAQFPVG
                     PMILIGHSDGGAMSLLYAASHPQRVLGIVGLAPQVGKPRQSAPVAAGASGAMQAVINA
                     FEKGELKRRLERYHGANTERMFYGWANAWSSPEFAGWQMSEELRQIRAPVVVIQGDRD
                     PYGYRHNIEALEQDLAAPLAVHIHPEAGHIPHHEARDFTLEHTVRAIRAFTA"
     gene            3578..4939
                     /locus_tag="PKI23_07525"
     CDS             3578..4939
                     /locus_tag="PKI23_07525"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNN36824.1"
                     /translation="MNLPVLNGVLSEIKGLAKSDDASIYQISEVILRDPNLTSQVLRI
                     ANTALYNPASVPINTVSRAAVMLGTGGILSIAISALVADSMNKGGRRERLMQAYGRSF
                     HAAVQARNLMAELKSHSQKEEIFIAGLLCNIGELTLWCCDDPLIDQLDQKLAEGTQSV
                     EQACMQTLGVQFKSLSRGLAKQWDLGEVLQDALSPTPRGRQAQAVLLGEQVSRAVESG
                     WDGPELGRLLPTLSKFMGCEPEAARESLLRSAQQAAEVVRDYGLPQAAQWILQPRAQS
                     AAVNNPLGTVVEVFAAAEQMTFERCDINGLLKVMVEGMHQALGLDRVVLAVLNGPHTR
                     LTGKYAAGSGCERLFGEFLFALDEGRNLFTELLATQKPMLVDSERNAEQRALLSEKMV
                     RLFGSGAFLLAPIGVGHRRFGLFYADRSVSGVPLTAADLAGFRQLAQHTTLCLGHLAA
                     ARG"
     gene            complement(4956..5933)
                     /locus_tag="PKI23_07530"
     CDS             complement(4956..5933)
                     /locus_tag="PKI23_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNN36825.1"
                     /translation="MSSTTAIVSVGEPWMWAAFVAFVLLMLALDLFVFGGRTAHRVSI
                     REAALWSAAWIGLALLFNLGLWWHLTDTVGPEIANRKALEFLTGYLIEKSLSVDNVFV
                     FLLIFSSFHVAAEYQRRVLIYGVVGAIAMRTVMILAGAWVVREFAWVLYLFGLLLVVT
                     GIRMLVMAERTPDLEKNPVLRFARRHLRISDDHHGEKFIVVKNGVRYFTPLFLVLILI
                     EASDLVFAVDSIPAIFAITTDPFIVFTSNIFAILGLRALYFLIADVASRFHLLKYGLA
                     MVLTFIGTKMLIAPWYHVPVQASLAIVAVLIGASVVASLIATREPAQGK"
     gene            complement(5947..6195)
                     /locus_tag="PKI23_07535"
     CDS             complement(5947..6195)
                     /locus_tag="PKI23_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNN36826.1"
                     /translation="MKCPVCKEPDLLLSERQGIEIDYCPQCRGVWLDRGELDKLIERS
                     DSPRGFDREQAAPRHERPYDDDAQRYKRRKSFLSELFD"
     gene            complement(6219..7097)
                     /gene="htpX"
                     /locus_tag="PKI23_07540"
     CDS             complement(6219..7097)
                     /gene="htpX"
                     /locus_tag="PKI23_07540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020191215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNN36827.1"
                     /translation="MKRIVLFLITNLAIVLVLSVSMRLLGVEPYLNAQGLNLDALLLF
                     AAIMGFGGAFISLALSKWMAKKSVGAQLITTPRTPTEQWLVQTVARQAQQAGVRMPEV
                     AIWEADEVNAFATGMSRNSSLVAVSTGLLRNMTQEEAEAVLAHEMAHVANGDMVTLAL
                     IQGVVNTFVLFLSRVIGHTVDRVIFRTESGQGPAFWVTVIIADLLLGLLASVIVMWFS
                     RQREFRADAGGARLASRPAMIAALQRLSSLHPQPLPDKMAAFGIAGGGAGGLKRLFMT
                     HPPLAERIEALQRGHD"
     gene            complement(7358..8911)
                     /gene="norR"
                     /locus_tag="PKI23_07545"
     CDS             complement(7358..8911)
                     /gene="norR"
                     /locus_tag="PKI23_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HNN36828.1"
                     /translation="MTTNPLLPAITRLVADLSLELPQSERYRRLLETLRALFPCDAAG
                     LLRLEQETLIPLAVDGLSADTLGRRFRLAEHPRLSAITSSGRPVRFAHDSELPDPYDG
                     LVIGFAEHLPIHDCLGVPLMIDGRLWGVLTLDSLEPGAFSALDMEALLAFVTLVCATV
                     KAAALIDALESRAEHARQAYLDLREATVTAAPQELIGESAAMRRLRDEIGVVAGTELT
                     VLITGETGVGKELVARAVHSRSLRSQQPLVELNCAAIPENLIESELFGHLKGAFSGAV
                     TERRGKFDLADGGSLFLDEVGELPLNAQAKLLRALHGGEIQRVGSDRVHRVDVRIIAA
                     TNRDLQQEVRNGRFRADLYHRLSVFPLWVPPLRERERDALLLAGYFLEQNRGRLGVRA
                     LRLAADCQAALLNCDWPGNVRELEHLISRAALRARAEAEADARIVTLHLHHLGMLNPP
                     AVEHPHARSEPDITQTVSLRQAVDDCQRDLLRQRLALHRGNWARTARSLGVDRGNLIR
                     LAQRLGLRN"
     gene            9067..10242
                     /gene="hmpA"
                     /locus_tag="PKI23_07550"
     CDS             9067..10242
                     /gene="hmpA"
                     /locus_tag="PKI23_07550"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HNN36829.1"
                     /translation="MLSAQTQALIKATVPALEAVNEKLTSHFYKIMFSEYPQVRALFN
                     QAHQQSGDQPRALANSVIAYAKHIDRLEALGSAVGLIVHKHASLNVLPEHYPIVGSCL
                     LRAIREVLGAAATDEIVDAWAQAYQQLADILIQAEEQVYAEHEQANGGWRGVRPLKVT
                     RKVKESDEITSFYLEATDGRPLLAATPGQYLALRFVMDGQEMRRNYSLSDVTDGSHYR
                     ISVKREPGGVISTHLHDRVAIGDLLEAFPPCGDFVLNPGQKPLLLLSGGVGLTPTVAM
                     LNAAVGSGRPIHFLHCARNRSVHAFREHVDGLAARHANLRRTYLYSQPAAADQADAVG
                     LISESVLAGALPADRDAEVYFIGPKPFMAEVKRLLAKLGVPEPQTHYEFFGPLQTLE"
     gene            10239..11186
                     /locus_tag="PKI23_07555"
     CDS             10239..11186
                     /locus_tag="PKI23_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNN36830.1"
                     /translation="MRRRERFTAWLGIEAAAPLRERLLSSVGGFIGLLAVAVLGFFHP
                     AAGDMPLLTGAMGATAMLLFALPHAVVSQPWPLFGGHLLSAIIGLGCARWLNHVPFTP
                     ALAVGLAIAAMHGARCLHPPGGATALIAAMNGPEVQALGWHYLLDPLLLHVGVLFLLA
                     MLFNAPFAWRRYPARWARAALSQGVLREEETLSSEDIAHAVRELGLVVDVDEVELRHI
                     LALALQRASSQHLLPELIVRGCCYSNGAHGEAWSVRQVLEMFHDQRLRYRSLVGPGEQ
                     FEAECGVAEFADWARYEVRMASGRWRRVQRSGATDDPQA"
     gene            complement(11208..12107)
                     /locus_tag="PKI23_07560"
     CDS             complement(11208..12107)
                     /locus_tag="PKI23_07560"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HNN36831.1"
                     /translation="MSPDSDSVTLTPAEPILSRCPNCATLFRVSPEQLHSAQGKVRCG
                     LCMAVFDAHGQQELASAVPPAVTLVEPQPTTTATTLPQSEQRTPARERRTSMPAAPTT
                     DIPVPLITAQTERPLLDRRLARQLQQLRLDEPLPTPTSRSRRPLIYGLLSLLALLLLA
                     AQLAWYRFDQLAVDPLLRPLFDRFCTLSGCSLPPQRDLQAMVVQQVAITPDPRRPEVV
                     TVEIILTNQAPFAQPFPAIELTFVDAADQPIAQRRLQPREYLSGELQDRTEMPSATPI
                     HLALPLIRPAQPAQQIKLRLVSP"
     gene            complement(12104..12982)
                     /gene="prmA"
                     /locus_tag="PKI23_07565"
     CDS             complement(12104..12982)
                     /gene="prmA"
                     /locus_tag="PKI23_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNN36832.1"
                     /translation="MPWWQLTLEADSPQCEALEALLLELGASAVTLDDAGDEPLFELA
                     PGNMPRWSQIRLTGLFADEIALLAALAQLPEPLPAYQISTLADEPWERSWMARFQPIR
                     CGERLWICPSWHQVDEPGAVVLQLDPGLAFGTGNHPTTQLCLGWLDSHPLAGLRVIDY
                     GCGSGILAIAALLLGAEQAVAIDIDPQALLATRENAERNQISTERLTTFTTEDEPAVV
                     ADLLLANILAGPLITLAPHLSQRLAPGGTLVLSGLLAEQVEAVAAAYRPWFDLAAPTF
                     AQEWVLLSGIKKADPT"
     gene            complement(12996..14336)
                     /gene="accC"
                     /locus_tag="PKI23_07570"
     CDS             complement(12996..14336)
                     /gene="accC"
                     /locus_tag="PKI23_07570"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNN36833.1"
                     /translation="MFNKIAIANRGEIALRILRACKELGIKTVALHSKADRDLMHVRL
                     ADETVCIGPSRPRESYLHAPAILSAMEVTDAEAVHPGYGFLSENADFAEQVEQCGFVF
                     IGPTASVIRTMGDKVSAIRTMKAAGIPTVPGSDGTLDDDSDRTLALAHQIGYPVIIKA
                     AAGGGGRGMRVVRSEAALLNAVQLTRSEAGAAFGDDRVYLEKFLERPRHVEIQVLADT
                     HGNVIHLGDRDCSLQRRHQKVLEEAPAPGIDPALRDRIAERCVQACREIGYRGAGTFE
                     FLYQDDEFYFIEMNTRVQVEHPVTEMVTGVDIVKEQIKIAAGQPLTLTQRDIVLRGHA
                     IECRVNAEDPRTFIPCPGEVKLFHAPGGNGIRVDSHLYTGYRVPADYDSLIGKLICLG
                     ENRDSAIARMRNALDETVINGIRTNLPLHQQILGNGNFQRGELSIHFLERLIES"
     gene            complement(14370..>14733)
                     /gene="accB"
                     /locus_tag="PKI23_07575"
     CDS             complement(14370..>14733)
                     /gene="accB"
                     /locus_tag="PKI23_07575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNN36834.1"
                     /translation="LPQQAFAQQPPIWQAAAPLAPPAAVPAAAPAPAAITPPPASSVQ
                     QAITSPMVGTFYRSPAPGSKAFVEVGQRINVGDVVCIVEAMKMMNQIEADKAGIVEAI
                     LVEDGAPVEFDQPLITVV"
BASE COUNT         2578 a         4385 c         5035 g         2735 t
ORIGIN      
        1 atgaagtctg tgcggccgcc ggtgctctgg accaatctgt tgctgtttgt catcccctcg
       61 acgttggcgc tggtggcggt gccgctctat gggtggtggc atggctatga tggtttcgag
      121 gtggggctct ttctgttttt tggcgccgcg agtggcacgg cgatcacggc ggggtttcac
      181 cgtctctggt cacaccaggc ttatgaagca cacccgctgc tgcggctctg gtttgcgctc
      241 tggggtgccg ccgcactgca aaacagcatt ctcaactggg cgtcggacca ccgtcgtcac
      301 caccgtcatg tcgatgatcc gcaactcgat ccctattcgg cacggcgcgg gttctggttt
      361 gcgcacatcg gctggatgtt gcgggagtat cgggcacagc aggacgattt cagcaatgta
      421 aaggatttgc agaaagaccc gatcgtgatg tggcagcacc gcaactacct gctgctggtg
      481 ctgctgatga acgtcgcgct gccggccctg attggcgtgg tgcatggcga tctgctgggg
      541 tcgctgctgc tggcgggggt gctgcggctg gtggtggtgc accatttcac cttcttcatc
      601 aattcgctgg cccatttctg ggggcgccaa ccctactcga gcgactgcac ggcgcgtgac
      661 aacgcggtgg tggcgttgct gacctttggc gagggctatc acaacttcca tcatcagttt
      721 cagtgggact accgcaatgg catccgctgg tggcagttcg atccgaccaa atggctgatc
      781 agggtctgtg cctggctggg actgtcgcgc aacctcaagc gctgttcgcc cgagaagatc
      841 gagcaggcga ttttgcagca acagttcagg cgctcatcac agcagatcag ccggctgccc
      901 gatgccgatt cgcacctgcg ccgcttgcag caggagtatg accgtctgat cgagatggtg
      961 caggcgtggg gcgagtcgcg ccagcgctgg gccgacgcgc gtgccaatca gttgttgcag
     1021 cagtgggagc gggcgcagat tcgtcgcagc tgtctggagc tgcggcagcg gctgcttgaa
     1081 cagcggcgcg attgggagct gttgctggcc cgcctgacct gagggtggaa cggacggttg
     1141 ccccgaatgc accatccatt cggggcagtg ccgtcaccct ctcatgaaga ggcttcgtcg
     1201 atgtcaccca tggcggtgat gttgaagcca ccatcgacat agaccacctc gccggtgatg
     1261 ccgctggcca attccgaaca gagaaaggcc gcagtgttgc cgacttcatg gatggtgaca
     1321 ttgcgccgca gcggggtgcg cgcctcgttg taggagagca tcttgcgaaa atccttgatg
     1381 cccgaggcgg ccagcgtgcg gatcggtccg gccgaaatcg cgttgacccg cacgccctgt
     1441 ggtcccaggc tgttggcgag gtagcgcacc gaggcttcga ggctggcttt ggccaggccc
     1501 atgacgttgt agttgggcat ggtgcgctgc gagccgagat aggtgagggt gagcagcgag
     1561 ccgtggctct tcaccagcat cggtcgggcg gccttggcca gcgccacgaa gctgtaggcg
     1621 ctgatgtcat gggcaatgcg gaaaccgtcg cgcgaggtga ccgcaaggta gtcgccgtcg
     1681 agctcctcgc gcggggcgaa gccgacacag tgcaccagaa tgtcgagtcc atcccacacc
     1741 ttgcccagtt cggtgaagac ggcgtcgatt tcatcatcgc tggcgacatc gcagggatag
     1801 cagagggtgg aatcccagcc tttggcaaac tcctcgaccc gctccttgag cttttcgccc
     1861 tgataggtga aggcgagttc ggcgccctcg cggtgcatcg cttcggcaat gccgtaggcg
     1921 atcgaaagtt tgctggcgac tccgacgatg agtgcgcgtt tgccggtgag aaagcccatg
     1981 gaggtgtcct ttggttattg tcgcgtgagt ggaatggccg aactggccgg cgtgatttca
     2041 gccacttgtc gccatttcag gaagtgctat tttagcaacc atcccagaag gagcgaaacg
     2101 aatgaacgat ctgacccaac agcagtgtga agcctgccgg atcggcgccc ccaaggtgac
     2161 cgaggccgag cgggccgagc tgctgccgaa gattcctgac tggaagatcg agcgcgatgg
     2221 ccacctcgac aagctgaagc gcgagtaccg gttcgacgac tacccgaagg cgctcgcctt
     2281 cacgcagcag attggcgaac tggccgaggc gcaggggcac cacccggcca ttctgaccga
     2341 gtggggcaag gtgacggtct ggtggtggtc gcacaagatc gaagggttgc accgcaacga
     2401 cttcatcatg gcggcgaaga gcgacctctg ttacgccacg aagggctgag tcgggtgact
     2461 ggctggcgca gactccaggg aggaagagat gattgatctc actgtagcgg aacggttcat
     2521 cgaggccggt ggccatcggc tcaaatgcac cacgctcggg cagcccgatc cggcccgtcc
     2581 gacgctggtt tttctgcacg aggggctggg ttcgatctcg atgtggaagg actatccggc
     2641 gcaggtggcc gagcaggtcg gactgccggt gctgatgtac gaccgctggg ggcatggcga
     2701 gtccgatccg ctgaccgagc cgcgcaagcc gagttatcgc cgggtcgagg cgctggaggt
     2761 gctgcccgag gtgctggcgc agtttccggt cgggccaatg atcctgatcg ggcacagtga
     2821 tggcggggcg atgagtctgc tctacgccgc gtcccatccg caacgggtgc tggggatcgt
     2881 cggcctggca ccgcaggtgg gcaagccacg gcagagtgcg ccggtggcgg ccggtgccag
     2941 cggcgcgatg caggcggtca tcaatgcctt cgaaaagggc gagctgaagc gccgtctcga
     3001 acgctaccat ggcgccaaca ccgagcggat gttctacggc tgggccaatg cctggagctc
     3061 cccggagttt gccggctggc agatgagcga agagctgcgg cagattcgcg caccggtggt
     3121 ggtgatccag ggtgaccgtg atccctatgg ctatcgccac aacatcgagg cgctggagca
     3181 ggatctggcc gcaccgctcg ccgtgcacat ccatcccgaa gcgggccaca ttccgcacca
     3241 tgaggcgcgt gacttcaccc tcgaacacac ggtgcgggcg attcgcgcct tcaccgcctg
     3301 attcttccga tttctgctcg aatcgtgatc ctgctcgcaa agctggacta gactgaaact
     3361 gatgcagcgt cactgctgaa accactgttc cgcgaggcca tggcggtttc gggtgtggcg
     3421 tgcatcaggg cgtgagcggg gacctcccgg ccactttgtg gttttccact tccagtttcc
     3481 agtttggagt caccccgtgg gtctgacggc agagctttct ccgctcaaaa gaacagcaga
     3541 tcaaccactc caggagtggc tcgcaaagat ccaggcgatg aatttgccgg tattgaatgg
     3601 ggtgctgagc gaaatcaagg ggctggccaa gagcgacgat gcgtcgatct accagatctc
     3661 cgaagtgatc ctgagagatc cgaatctgac ctcccaggtg ctgcgcatcg ccaataccgc
     3721 gctctacaac cccgcatcgg tgccgatcaa cacggtcagc cgcgcggcgg tcatgctcgg
     3781 caccggcggc attctctcga tcgccatctc cgcgctggtg gccgattcga tgaataaagg
     3841 cgggcggcgc gagcggctga tgcaggccta cggacgctca tttcatgccg cagtgcaggc
     3901 gcgcaacctg atggccgagc tgaagtcgca cagccagaaa gaggagatct tcattgccgg
     3961 gctgctctgc aacatcggcg agctgacgct gtggtgctgc gacgatccac tcatcgacca
     4021 gctcgatcaa aaactcgccg agggcacgca gagcgtcgaa caggcctgca tgcagaccct
     4081 cggtgtgcag ttcaaatcgc tgagtcgggg gctggccaag cagtgggatc tgggtgaggt
     4141 gctgcaggat gccctctcgc cgacgccgcg cgggcggcag gcgcaggcgg tgctgctggg
     4201 tgagcaggtg agccgtgcgg tcgagtctgg ctgggatgga ccagagcttg gacggctgtt
     4261 gccgaccctg tcgaaattca tgggctgcga gcccgaggca gcgcgtgagt cgctgctcag
     4321 gagtgcacag caggcggccg aggtggtgcg cgattacggc ctgccacagg cggcacaatg
     4381 gattctgcag ccgcgtgccc agtctgccgc agtgaacaat ccgctgggaa cggtggtgga
     4441 ggtgtttgcc gccgccgagc agatgacctt cgagcggtgc gacatcaatg gcctgctgaa
     4501 ggtgatggtc gaggggatgc atcaggcgct ggggctcgat cgggtggttc tggctgtgct
     4561 caatggcccc cacacccgtc tgaccggcaa gtacgccgcg ggcagcgggt gcgaacggct
     4621 gttcggggag tttttgtttg cgctcgatga gggcaggaat ctcttcaccg aactgctggc
     4681 cacgcaaaag ccgatgctgg tcgacagcga acgcaatgct gagcagcggg ccctgctgag
     4741 cgaaaagatg gttcgcttgt tcggttccgg cgcttttctg ctggcgccga tcggggtcgg
     4801 gcaccgccgt ttcggcctgt tctatgccga ccgcagcgtc agtggcgtgc cactgacagc
     4861 ggccgatctg gccgggttcc gccaactggc ccagcacacg acgctctgtc tgggccatct
     4921 ggctgccgcg cgcggctgat ccatccccct cttcatcact ttccctgcgc tggttcgcgg
     4981 gtggcgatca ggctggcgac cacgctggcg ccgatcagca ccgccacgat ggcgagcgac
     5041 gcctgcaccg gaacatggta ccagggggcg atcagcatct tggtgccgat gaaggtgagc
     5101 accatcgcca gtccatattt gagcagatgg aagcggctgg ccacatcggc gatcagaaaa
     5161 tagagcgccc gcaaccccag aatcgcaaag atgttggagg tgaagacgat gaaggggtcg
     5221 gtggtgatgg cgaaaatggc cggaatggag tcgacggcga acaccaggtc cgaggcctcg
     5281 atcagaataa gtaccaggaa cagcggggtg aagtaacgca ccccgttctt gaccacgatg
     5341 aatttctcgc cgtggtgatc gtcgctgatg cgcaggtggc gccgggcgaa gcgcagcact
     5401 ggattctttt cgaggtcggg cgttctttcc gccatcacca gcatccggat gccggtcacc
     5461 accagcagca gaccgaacag atagagtacc caggcgaact cgcgcaccac ccaggcgccg
     5521 gccagaatca ttaccgtgcg catggcgatg gcgccaacca cgccatagat cagcacacgc
     5581 cgctggtatt cggccgcaac gtgaaatgac gagaagatca gcaggaagac aaagacattg
     5641 tcgaccgaga gtgatttctc gatcaggtag ccggtcagaa actccagcgc cttgcggttg
     5701 gcgatctccg gcccgacggt gtcggtgaga tgccaccaga gtcccaggtt gaagagcagt
     5761 gccaggccga tccatgcagc ggaccagagc gcggcctccc tgatgctcac ccggtgtgcg
     5821 gtgcggccgc cgaacacgaa caggtcgagt gccagcatca gcagcacgaa agccacgaag
     5881 gctgcccaca tccagggttc gccgaccgat acgatggctg tcgtgctgct catatggagt
     5941 ctcccctcag tcgaacagtt cgctcagaaa ggatttgcgc cgcttgtagc gctgcgcgtc
     6001 gtcatcgtag gggcgctcgt gtcggggtgc ggcctgttca cggtcgaagc cgcgtggcga
     6061 gtccgaacgt tcgatcagct tgtccaattc accgcgatcg agccacacgc cacggcattg
     6121 cgggcaatag tcgatctcga tgccttggcg ttcgctcagc agcagatcag gttccttgca
     6181 gactgggcat ttcatgcgct ttctcctggt gggtggtttc agtcgtgacc gcgttgcagc
     6241 gcttcgatgc gttcggcaag cgggggatgg gtcatgaaca gacgtttcag gccgcccgcg
     6301 ccgccgccgg cgatgccgaa ggcagccatc ttgtccggca gtggttgggg gtgcaagctg
     6361 gagagccgtt gcagtgccgc gatcattgcc gggcgactgg ccagccgggc gccgccggca
     6421 tcggcacgaa actcccgctg ccgcgagaac cacatgacaa tcacagaggc gagcagcccg
     6481 agcagcaggt cggcgatgat cacagtcacc cagaaggccg gcccctggcc gctctcggtg
     6541 cggaagatca cccggtcgac ggtgtggccg atcacccgcg acagaaacag cacgaaggtg
     6601 ttcaccacgc cctggatcag cgccagcgtc accatgtcgc cgttggcgac gtgggccatc
     6661 tcgtgcgcca gcaccgcctc ggcttcctcc tgggtcatgt tgcgcagcaa tccggtcgag
     6721 accgccacca gcgaactgtt ccggctcatg ccggtggcga aggcattgac ctcgtccgct
     6781 tcccagatgg cgacctcggg catcctgacg ccagcctgct gcgcctgacg tgcgaccgtc
     6841 tgtaccagcc actgctcggt gggtgtgcgt ggcgtggtga tcagttgcgc accgaccgat
     6901 tttttggcca tccatttgga gagggcgagc gagatgaaag cgccgccaaa gcccatgatg
     6961 gcggcgaaca gcagcagcgc atcgaggttc aatccctgtg cattcaggta gggctccacg
     7021 ccgagcagcc gcatgctgac cgagagcacc agcacgatgg cgaggttggt gatcagaaaa
     7081 agtacgatgc gtttcatggc cgagactcct ggtggcggtt gggtaaagtg ccatttgccg
     7141 tgcctggact gcgcggaagg tagcgtgagc tctgtgttcg catggatttt gctgcgcctt
     7201 tgggttttga cgcagccatt ctcgagccgt tttttacgaa caaaaagcag attattttct
     7261 gctttttgtt cgtaaaaaac tgatttgtga ggtgcgggtt ccggtcaggg ccggatgcat
     7321 gggagcatgg cgcgatcggg cgtcgaaatc tgccgcttca gttgcgcaat ccaaggcgct
     7381 gggcgaggcg aatcaggttg ccgcggtcga caccgagact gcgtgcggtc ctggcccagt
     7441 tgccgcggtg cagcgccagc cgttgtcgca gcagatcgcg ctggcagtca tcgacggcct
     7501 ggcgcagtga gacagtctgg gtgatgtccg gctcggatcg ggcgtggggg tgttcgactg
     7561 ctggcgggtt cagcatgccg aggtggtgca ggtggagggt gacgatccgc gcgtcagctt
     7621 cggcttcggc gcgggcacgc agggcggcgc ggctgatcag atgctccagt tcacgcacat
     7681 tgccgggcca gtcacagttg agcagggcag cctggcagtc ggccgccaac cgcagggcgc
     7741 gcacgcccag acggccgcgg ttctgctcca gaaagtagcc ggccagcagc agggcatcgc
     7801 gttcgcgttc gcgcaggggc ggcacccaca gcggaaatac gctgagtcga tggtagagat
     7861 cggcccgaaa gcgtccgttg cgcacctcct gctgcaggtc gcggttggtg gcagcgatga
     7921 tgcgcacatc gacccgatga acgcggtcac tgccgacacg ctgaatttcg ccgccgtgca
     7981 gcgcgcgcag cagcttggcc tgggcgttca gcggcagttc accgacttca tcgagaaaca
     8041 gactgccgcc atccgccagg tcgaacttgc cacgacgctc ggtcaccgca ccagagaagg
     8101 cccccttcag atggccgaac agctcgcttt cgatcagatt ctcgggtatg gccgcgcagt
     8161 tcagctcgac cagcggctgt tggctgcgca gtgatcggct gtgaacggcc cgcgccacca
     8221 actccttgcc gacgccggtc tcgccggtga tcagcacggt cagctcggta ccggccacca
     8281 caccgatttc gtcacgcaga cggcgcatgg cggcgctctc gccgatcagc tcttgcggcg
     8341 cagcggtgac ggtggcctcg cgcagatcga ggtaggcctg tcgcgcatgt tcggcgcggg
     8401 attcgagtgc atcgatcagc gcggcggcct tgaccgtggc gcagaccagc gtgacgaagg
     8461 cgagcagcgc ctccatgtcc agcgcggaaa aggcgccggg ttccagcgaa tcgagtgtca
     8521 acacacccca gagcctgcca tcgatcatca gcggcacgcc cagacaatca tgaatcggca
     8581 gatgttcggc aaagccgatc accaggccgt cgtaggggtc cggcagctct gagtcgtggg
     8641 cgaagcgaac cggtctgccg gacgaggtga tggcggagag acggggatgc tcggcgaggc
     8701 ggaagcgccg tcccagggtg tcagccgaaa ggccatcgac cgccagtgga atcagtgtct
     8761 cctgctccag ccgcagcaac ccggcggcat cacagggaaa gagtgcgcgc aaggtttcga
     8821 gcaggcgacg atagcgctca ctctgcggca gttcgaggga gaggtcggcg accaggcggg
     8881 tgatggctgg aagcagcgga tttgttgtca tttaaacact ttatgtcgat ttatgctgtc
     8941 ataaagagac tgtcatcatg acacgaaaag caattaatgg tctgttttta aactgatttg
     9001 gttgtggcac atctcttgta atgtctctgt tcgaacgctt cactcaaacc tgcagaggca
     9061 tgctccatgt tgtcagcaca gacccaggcc ttgatcaagg caaccgttcc ggccctcgag
     9121 gcggtcaatg aaaaattgac cagccacttc tacaagatca tgttcagcga atatccgcag
     9181 gtgcgggcac tgttcaatca ggcgcaccag cagagcggcg atcagccgcg ggcgctggcc
     9241 aacagtgtga tcgcctatgc caaacacatc gatcggctcg aagcgctggg atcggccgtg
     9301 ggcctgatcg tgcacaagca cgcttcgctg aatgtgctgc cggagcacta tccgattgtc
     9361 ggcagctgcc tgctgcgggc gatcagggag gtgctgggcg cggccgccac cgacgagatc
     9421 gtcgatgcct gggcgcaggc ctatcagcag ttggccgaca ttctgattca ggccgaggag
     9481 caggtctacg ccgagcatga gcaggccaat ggcggctggc ggggcgttcg cccgttgaag
     9541 gtgacacgca aggtaaagga gagtgacgag atcacctctt tttatctcga agcgaccgac
     9601 ggccggccgc tgctggcggc cacccccggg caatatctgg cgctgcgctt cgtgatggat
     9661 gggcaggaga tgcgccgcaa ctattcgctc tcggatgtca ccgatggcag ccactaccgc
     9721 atcagcgtca agcgcgagcc cggcggggtc atctccaccc atctgcatga ccgggtggcg
     9781 attggcgatc tgctggaggc tttccctccc tgcggtgatt ttgtgctcaa cccgggccag
     9841 aaaccgctgc tgctgctgag cggtggtgtg ggattgacgc cgacggtggc gatgttgaac
     9901 gcagcggtgg gcagtggtcg gccgatccat tttctgcact gtgcacgcaa tcggtcggtg
     9961 catgcctttc gtgagcatgt cgacgggctg gccgcccgcc atgccaacct gcgccgaacc
    10021 tatctctaca gccagccggc agcggccgac caggccgatg ccgtcggtct gatcagtgag
    10081 tcagtgctgg ccggtgcact gccggcggac cgggatgccg aggtctattt catcgggccg
    10141 aagcccttca tggccgaggt caagcggctg ctggccaagc tgggtgtgcc cgaaccgcag
    10201 acccattatg agttcttcgg gccattgcag acgttggaat gaggcgaaga gagcgattca
    10261 ccgcctggct tggcatcgag gccgccgcgc cgctgaggga gcggctgctc tcttctgttg
    10321 gcggattcat cggcctgctg gcggtggccg tgctgggatt cttccatccg gcggccggcg
    10381 acatgccgct gctgaccggg gcgatggggg ccactgccat gctgctgttc gcgctgccgc
    10441 atgcggtggt ctctcagcct tggccgctgt tcggtggcca tctgctgtcg gccatcatcg
    10501 gtctgggctg tgcgcgctgg ttgaaccatg tgcctttcac acccgcattg gcggtggggc
    10561 tcgccatcgc agccatgcat ggtgcccgct gtctgcatcc gccgggtggt gccacggcat
    10621 tgatcgccgc gatgaatggt cccgaggtgc aggcactggg ctggcactac ctgctcgatc
    10681 cgctgctgct ccatgtcggc gttctgttcc tgctggcgat gctgttcaac gccccctttg
    10741 cctggcggcg ctatccggcg cgctgggcgc gggcagcgct ctcgcagggt gtcttgcgtg
    10801 aggaggagac cctctcttcc gaagacattg cccatgccgt gcgtgaactg gggctggtgg
    10861 tggacgtcga tgaggtcgaa ctgcgacaca ttctggcgct ggcactccag cgcgccagca
    10921 gccagcatct gctaccggaa ctgatcgtgc gcggctgctg ttacagcaat ggcgcgcatg
    10981 gggaggcctg gtcggtgcgt caggtgctgg agatgttcca cgaccagcga ctgcgctacc
    11041 gcagtctggt ggggccgggc gagcagttcg aggccgaatg cggcgtggcg gagtttgcgg
    11101 actgggcgcg ttacgaggtg cgcatggcgt cgggtcgctg gcggcgggtg cagcgttcgg
    11161 gagccacgga cgatccgcaa gcgtgaattc gctcggcaac gctcccgtca gggcgagacg
    11221 aggcgcaatt tgatctgctg cgcgggctgt gccggacgga tcagcggcag cgcgagatgg
    11281 atcggtgtgg cgctcggcat ttccgtgcgg tcttgcaatt caccactcag atactctcgc
    11341 ggctgcagtc ggcgctgggc gatcggctgg tcggccgcat cgacgaaggt gagttcgatg
    11401 gccggaaacg gctgtgcgaa gggcgcctga ttggtgagga tgatctccac ggtcaccact
    11461 tcgggccggc gtggatcggg ggtgatggcg acctgctgca cgaccatggc ctgcaggtcg
    11521 cgctgcggcg gcaggctgca tccgctgagt gtgcagaagc ggtcgaacag cggacgcagc
    11581 agcggatcga ccgccagttg gtcgaagcgg taccaggcca actgcgcggc gagcagcagc
    11641 agcgccagca gcgacaggag gccatagatc agtggccgcc tggagcgcga tgtgggcgtc
    11701 ggcagcggtt catcgagtcg aagctgctgc aactgccggg caaggcgccg atcgagcagc
    11761 ggccgctcgg tctgcgcggt gatgagcgga accgggatgt cagtggtggg tgctgccggc
    11821 atgctggtgc gccgttcgcg ggcgggcgtg cgctgctcgc tctggggcag tgtcgtggcg
    11881 gttgtggtcg gctgaggttc gacgagcgtg accgctggcg gcaccgcgct ggccagctcc
    11941 tgctggccat gcgcgtcgaa gacagccatg cacagcccgc aacgcacctt gccctgggcc
    12001 gagtgcaact gctccgggct gacgcgaaac agtgtcgcgc agttggggca gcggctgagg
    12061 atcggctctg ccggcgtcag ggtgacgctg tcactgtccg gactcatgtc ggatcagcct
    12121 ttttgatgcc gctcagcagg acccactcct gcgcgaaggt cggcgcagcg agatcgaacc
    12181 agggtcgata ggccgcagcc acggcctcga cctgttcggc cagcagacca ctgagtacca
    12241 gtgtgccacc cggtgccagc cgctggctca ggtgcggggc cagggtgatc agcggtccag
    12301 ccaggatgtt ggccagcagc agatcggcca ccacggcagg ttcgtcttcg gtggtgaagg
    12361 tggtgagccg ttcagtggag atctggttgc gttcggcatt ctcgcgggtg gccagcagcg
    12421 cctgcggatc gatgtcgatg gccactgcct gctcggcgcc cagcagcagc gcggcgatgg
    12481 cgaggatgcc ggagccgcag ccgtagtcga tgacccgcag gccggcgagc gggtggctgt
    12541 cgagccagcc gagacagagc tgggtggttg gatggttgcc ggtgccaaag gccaggccag
    12601 gatcgagctg cagcaccacc gcgccgggtt cgtccacctg atgccagctt gggcagatcc
    12661 agagccgctc accgcagcga atcggctgaa agcgcgccat ccagctgcgc tcccagggtt
    12721 catcggccag cgtactgatc tggtaggcgg gcagtggttc gggcagttgc gccagcgcgg
    12781 ccagcagcgc gatctcatcg gcaaacagac cggtcagccg aatctggctc cagcgcggca
    12841 tgttgccggg tgccagctcg aacagcggct cgtcaccggc atcatccagc gtcaccgccg
    12901 aggcgcccag ttcgagcagc agcgcttcga gcgcctcgca ctgcggtgag tcggcctcca
    12961 gtgtcaactg ccaccagggc atggcgtgct ccgtgtcagg attcgatcag ccgttcgaga
    13021 aagtggatgc tcaactcgcc acgctggaag ttgccattgc cgagaatctg ctggtgcagc
    13081 ggcaggttgg tgcggatgcc gttgatcacg gtctcgtcga gggcattgcg catgcgggcg
    13141 atggcgctgt cgcggttctc gcccaggcag atcagtttgc cgatcagcga gtcatagtcg
    13201 gcggggacgc ggtagccggt gtagaggtgg gagtcgacac ggatgccatt gccgcccggc
    13261 gcatgaaaca gcttgacctc gccggggcag gggatgaagg ttctgggatc ctcggcattg
    13321 acccggcact cgatggcgtg gccgcgcagc acgatgtcgc gctgggtcag cgtcagcggc
    13381 tgaccggcgg cgatcttgat ctgctccttg acgatgtcga cgccggtgac catctcggtg
    13441 accggatgct cgacctgcac gcgggtgttc atctcgatga agtagaactc atcgtcctgg
    13501 tagagaaact cgaaggtgcc ggcaccgcgg tagccgatct cgcggcaggc ctgcacgcag
    13561 cgctcggcga tgcggtcgcg cagagcggga tcgatgccgg gcgcgggggc ctcttcgagc
    13621 accttctgat gacgtcgctg caacgagcag tcgcggtcgc ccaggtggat cacattgccg
    13681 tgggtgtcgg ccagcacctg aatctcgaca tggcgcggcc gttcgaggaa tttctcgaga
    13741 tagacccggt cgtcgccgaa cgcggcaccg gcctcgctgc gggtcagttg aacggcattg
    13801 agcagcgcgg cctcgcttct gaccacccgc atgccgcggc caccgccacc ggcggcggcc
    13861 ttgatgatga ccggatagcc gatctgatgc gccagcgcca gggtgcggtc gctgtcgtca
    13921 tcgagggtgc cgtcggagcc ggggaccgtg ggaatgccgg ctgctttcat ggtgcggatg
    13981 gccgagacct tgtcgcccat ggtgcggatc acgctggcgg ttgggccgat gaagacgaag
    14041 ccgcactgct cgacctgttc ggcgaagtcg gcgttttcag agagaaaacc gtaaccggga
    14101 tgcaccgcct cggcgtcggt cacctccatg gcgctgagga tggccggcgc gtggaggtag
    14161 ctctcgcgcg gccggctggg gccgatgcag acggtctcgt cggccagacg cacatgcatc
    14221 agatcgcggt cggccttgga gtgcagcgcc acggtcttga tgccaagctc cttgcaggca
    14281 cgcaggatgc gcagggcgat ctcgccgcgg ttggcgatgg caattttgtt gaacatggat
    14341 ggagcggttc cggagtctga gagatcggcc tagacgactg tgatcagtgg ctggtcgaat
    14401 tcgaccgggg cgccatcttc gaccaggatg gcctcgacga tgccggcctt gtcggcttcg
    14461 atctggttca tcatcttcat tgcctcgacg atgcagacca catcgccgac attgatccgc
    14521 tgacccacct cgacgaacgc cttgctgccc ggtgccggtg agcgatagaa ggtgcccacc
    14581 atcggcgagg tgatggcctg ctgcacactg ctggctggcg gcggcgtgat tgccgccggg
    14641 gcgggcgcgg ctgccggcac cgctgcgggc ggggccagcg gcgcggcggc ctgccagatc
    14701 ggcggctgct gggcgaaagc ctgttgtggc agg
//
LOCUS       DAORCQ010000060        14507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2673_length_14507_cov_5.675893, whole genome shotgun sequence.
ACCESSION   DAORCQ010000060 DAORCQ010000000
VERSION     DAORCQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14507
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_14507_cov_5.675893"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKI23_07580"
     CDS             complement(<1..374)
                     /locus_tag="PKI23_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HNN36835.1"
                     /translation="MYCLPLCLLFNRLFVPSHATLLQGGGEEPIYLPATPGQPLHLIR
                     FRHDYFASALHEVAHWCIAGPTRRQQVDYGYWYCPDGRNADEQARFEQVEAVPQAVEW
                     LLTLATGARFRLSLDNLNGALPS"
     gene            complement(443..919)
                     /gene="greA"
                     /locus_tag="PKI23_07585"
     CDS             complement(443..919)
                     /gene="greA"
                     /locus_tag="PKI23_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNN36836.1"
                     /translation="MKRVPMTKEGAKQLEEELQRLKTVERPRIINAIAEARAHGDLKE
                     NAEYHAAREQQSFTEGRIREIESKLSEAQIIDITQIPNSGKVIFGATVDLINSATDER
                     LTFRIVGDDEADVKANKISVSSPLARALIGKEEGDIVEVATPGGKVSYEIDATRHQ"
     gene            complement(919..4137)
                     /gene="carB"
                     /locus_tag="PKI23_07590"
     CDS             complement(919..4137)
                     /gene="carB"
                     /locus_tag="PKI23_07590"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNN36837.1"
                     /translation="MPKRTDIGSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPVMADATYIEPINWRTVAKIIEQERPDALLPTMGGQTALNCALDL
                     DRHGILAQYGVEMIGANREAIDTAEDRQLFDRAMRSIGLATPRSAIAHSLEEAEQVQG
                     QIGYPCIIRPSFTMGGSGGGIAYNREEFIEICQRGLALSPTRELLIDESLIGWKEFEL
                     EVVRDRADNCIVVCTIENFDPMGVHTGDSITVAPAQTLTDKEYQLLRDAAIRVLRRIG
                     VETGGSNVQFGVDPATGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNEITGGLIPTSFEPAIDYVVTKIPRFTFEKFAEANNILTTQMKSVGEVMAIGRSFQ
                     ESLHKAMRGLETGVMGFDPKLDLGARDAQEQLRQRLINPTAERLWYIGDAFRAGMGVD
                     EIHELTAIDPWFLAYIDDLIREEQALVGQPLAALDADWLRRLKRKGFADARLARLLGV
                     DEQAVRDQRHRLGVRPVYKRVDTCAAEFAASTAYLYSSYDEECEAAPSQRDKIMVLGG
                     GPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVALEQPKGVIVQFGGQTPLNLARALHRAGVPIIGTTPDAIDRAEDRERFQQMIQ
                     QLNLRQPPNRTVRSLEEGLVAAESIGYPLVVRPSYVLGGRAMEIVYNTAELKRYMVEA
                     VQASSERPVLLDRFLDDAVEVDIDAVSDGRQVVIGAIMQHIEQAGVHSGDSACALPPY
                     SLAPELQQRMRGMIEAMALELGVVGLMNTQLAVQNGEIYVLEVNPRASRTVPFVSKCI
                     GISLAKVAARCMAGIDLAQQGFVTERIPDYVSVKEAVFPFNKFPGVDPILGPEMKSTG
                     EVMGVGLTFAEAFAKAQAGAGELLPSGGVAFLSVRDPDKAGAVEVARALIGLGFSLTA
                     TRGTAAALKAAGLAVEQTNKVGEGRPHIVDRLKNGEISFIVNTTEGRQAIADSATIRR
                     YALLNRVHYTTTLAGAEAICQALQFKGDLAVHTLQALHERLK"
     gene            complement(4167..5312)
                     /gene="carA"
                     /locus_tag="PKI23_07595"
     CDS             complement(4167..5312)
                     /gene="carA"
                     /locus_tag="PKI23_07595"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNN36838.1"
                     /translation="MSARPAVLILEDGSCFRGMSIGADGMAVGEVVFNTSMSGYQEIL
                     TDPSYARQIVTLTYPHIGNTGITAEDEESPSIHAAGLVIRDLPLRSSNWRRQGELDEY
                     LRHHRRVGIAGLDTRRLTRILRDQGAQRGCIMAGAELDQSAALTAARAFPGLQGMDLA
                     KEVTTARAYPWSEGCWRISTGHRVVTESRHHVVAYDFGVKRNILRMLAERGCRVTVVP
                     ATTSATEVLALQPDGVFLSNGPGDPEPCDYAIAAIREFLQRDLPLFGICLGHQLLALA
                     SGARTLKMKFGHHGANHPVQDLRSGKVMITSQNHGFAVDSEDLPDNIEVTHLSLFDGS
                     VQGFARRDRLAFGFQGHPEASPGPHDAEALFDHFISLIDLHRQRQVN"
     gene            5575..6165
                     /locus_tag="PKI23_07600"
     CDS             5575..6165
                     /locus_tag="PKI23_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNN36839.1"
                     /translation="MNDIPASTLPAPMPIEPADTSVKAALSHFFEQHETSAYLRARRV
                     TRDSDTALELVQEAMTRLVSHYRDRPPAQWPALFQRILQNQLRDWQRQQYGPRAGWRF
                     AAYDEQDEQNPTLPAASSLEPDRAMARGRFHAALAQAVASLAPQQQRTFMLRCWDGLN
                     EQQTADRLGISIGSVKTHYFRALQQLRARLEAYRHE"
     gene            6158..6418
                     /locus_tag="PKI23_07605"
     CDS             6158..6418
                     /locus_tag="PKI23_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36840.1"
                     /translation="MSDEQESIPAEIHQRLRQARLQAIENGLRQRPRRLPERFWMAAT
                     AVMLALLLTHRPPPQDDSPQQSAELLAEVEFYQWLGLTAEAG"
     gene            6622..7848
                     /locus_tag="PKI23_07610"
     CDS             6622..7848
                     /locus_tag="PKI23_07610"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNN36841.1"
                     /translation="MRKLLLPALISAASLSIATSALAGTVTTDGSDIIIKTKGGLEAK
                     TADGKASFKLGGRVQLDYNGYDGVINTKTPGENGSDLFFRRARLEMGGTYKDWGYLMS
                     YNLTESGSIDLLWTSYNGFGKLAELTFGQQKEDFGLEDTGSSKWITAIERSMPSNAFD
                     TKNNVGVKLHGANKTFSYSLGAYKQDLDNSNDLNSALTGRFVVRPIYGKDRLLHLGVG
                     ATSRDGKFTKISSRLGVRGNEDGSAAQVVSAAYTGTPAPVADGQDVWNAELAGYLGPV
                     HLMAEYFDGKISGVNSSPDIEANGYYVQLGWIVTGEHRRYNDEIAAFDKVKPVNPQGA
                     WEIFARYDELDASDNQNVAVKMVGETGNTTTLGVNWYANDLVKVGLNYVHAKTDQPIG
                     GEDSGDALVSRLQIAF"
     gene            complement(7953..8234)
                     /locus_tag="PKI23_07615"
     CDS             complement(7953..8234)
                     /locus_tag="PKI23_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36842.1"
                     /translation="MAKKSGGGDLLDGVNRGDFDYDYIGSKDESAEARTMSSRARLRQ
                     KMQSDVEQFLKRGGTIDVVENTLRQPAKKPKVEYGSEPISAEGGVEEIE"
     gene            complement(8491..9009)
                     /locus_tag="PKI23_07620"
     CDS             complement(8491..9009)
                     /locus_tag="PKI23_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNN36843.1"
                     /translation="MSEHVVYFAHGKESGPWGIKITALAQVARELGLAVESPDYQGLS
                     GPARVDKLLALLNDESRQPILVGSSMGGYVAAATANLREVAGLFLLAPALYIDRPDYP
                     QSHFVPRTRQRVVIHGWRDEIVPVAAALRFASEVECDLHLLLSGHDLVDRLTDLEQLF
                     RLFLARILGAQT"
     gene            complement(9023..9955)
                     /locus_tag="PKI23_07625"
     CDS             complement(9023..9955)
                     /locus_tag="PKI23_07625"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNN36844.1"
                     /translation="MTTATMADRDGLIWLDGEMVPWRDARVHVLTHTLHYGMGVFEGT
                     RAYRTDRGPCIFRLVDHTERLFRSAHIMNMKIPYTQQQLNAAQLELIRVNDLPEAYIR
                     PMAFYGAEGMGLRADNLKVHVMIAAWVWSSYMSPEALVNGIKVRTSSYTRNHVNIAMC
                     KAKANGNYINSMLALQEALGSGADEALLLDSEGYVSEGSGENFFMVSRGVLYTPELTS
                     CLDGITRRTVCTLAEEEGLKIVEKRITRDEVYVADEAFFTGTAAEVTPIQSLDGRIIG
                     SGTRGPITTRLQQLYFDEVAARRDGHPEWHSFVN"
     gene            complement(10003..12912)
                     /gene="glnE"
                     /locus_tag="PKI23_07630"
     CDS             complement(10003..12912)
                     /gene="glnE"
                     /locus_tag="PKI23_07630"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNN36845.1"
                     /translation="MNPPIDFLPAALQAVVLRHWQRLDDATMQRYLALPPARLERLLR
                     VWAVSDFVADCCVTQPMLALELEASGDLDRDYPSGMYQAQAKQLLTVVDSEAALMQQL
                     RRWRRREMVRIACRDRCRLADFVTTSRELSALAEAAVDVALGWLYQDGCRSHGVPTGR
                     HTGREAVMVVLGMGKLGGGELNFSSDIDLIFACSEAGETRGGPQSIDNGRFFTRLGQR
                     LINVLDRATDEGSVFRVDMRLRPYGQSGALVPSFDAMESYYQEQGRVWERYAMLKARV
                     IAGAPAAAADLMARLTPFVYRRYLDFSVIGALRELKAMIQREVREKGLEQNLKLGSGG
                     IREVEFIVQALQLSHGGRDPRLRQTSLLAALGQIADGGYLPQPTVAALREAYLFLRDS
                     EHVLQELDDRQTQNLPTDEAERERVAFAMGFADWSGFQQRLTEYRETVHRHFNEMVSE
                     PEAKSALPTPEGSGWLKLWRGELAEDQAARWLTEQGLQQPQALMQRIAQLRSEPRVAA
                     LRDEARARLDRLLPMLLADIARHATPQVALERVLTVIEALLRRTVYLVLLTENPSALH
                     HLIDLCALSPWITDLISRFPSLLDEFLDAQNLYAPPERQAVQEALNLRLLRIPEDDLE
                     AQMEGLRYFKQAHLLKVAAAEMAQRIPLMKASDYLTFIAEAVLVQVFNLAWRHLTRKH
                     GVPQRAPGVACNPDFIIVGYGKLGGIELSYSSDLDLVFIHDAAPNLTTDGDQPIDNGL
                     FFSRLVARMMHILTAVTPSGQLYEVDIRLRPSGRSGVLVSSLNAFRGYQLEDAWTWEH
                     QALVRARVVAGCHRLRQKFEQVRAEVLGRPRDLPTLRDEVAKMRGRMVEELGSVDRHQ
                     AIDEAALFDLKQDPGGIVDIEFLAQYGVLAWSADHPQLLQFTDTIRILDGLEEAALLG
                     ADEARQLRDHYRSYRTSTHRLALMKEPQVVPAAPWRDARRQVAAIWQRLLVPPAA"
     gene            complement(12926..13618)
                     /locus_tag="PKI23_07635"
     CDS             complement(12926..13618)
                     /locus_tag="PKI23_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNN36846.1"
                     /translation="MTAPRHRRHYRYYDLVMAAFVTVLLCANLIGVSKVTTVAGFTFS
                     AGNLFFPISYLFGDILTEVYGFARSRRVVWAGFGALAFASFMSTVVVNMPPAAGWEGQ
                     AVIEAAFGSTWRIALASLIGYWCGEFTNSFTLARMKVLTQGRHLWSRTIGSTVAGEAV
                     DTLVFYPLAFYGVWETDLLLAVMAMNYCIKVGWEVVMTPLTYRIVAALKRAEHEDYFD
                     DKTDFNPFSLKV"
     gene            13807..14277
                     /locus_tag="PKI23_07640"
     CDS             13807..14277
                     /locus_tag="PKI23_07640"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNN36847.1"
                     /translation="MHHAATLHSPLGELTLCASDHALTAIRFPEEPPPALPVAGPNAL
                     LREAIRQLTAYFDGRLQQFDLPLAGEGSPFQQRVWQALLQVPHGRTASYGAIAAAIGN
                     PRACRAVGLANGRNPIAIVVPCHRIIGRNGQLTGYGGGLERKRWLLRHEGVGVN"
BASE COUNT         2560 a         4821 c         4619 g         2507 t
ORIGIN      
        1 gaggggaggg cgccgttcag gttgtcgagg ctgagccgga aacgggcgcc ggtggcgagg
       61 gtgagcagcc actccaccgc ctgcggcacc gcctccacct gctcgaagcg ggcctgctca
      121 tcggcattgc ggccatccgg gcagtaccag tagccatagt cgacctgctg gcgccgggtc
      181 gggccggcga tgcaccagtg tgccacctca tgcagcgcgc tggcgaagta gtcatggcga
      241 aagcggatca gatgcagtgg ctgtcccggc gtggccggca ggtagatcgg ctcctcgccg
      301 ccaccctgca gcagggtggc atggctggga acgaacaggc ggttgaacag caggcagagg
      361 gggaggcagt acatgacggc atgatcctgc aaggctgacg ccgtacccgg cctgcctgcg
      421 tcatcggcca tccgcggccg cgctactgat gccgggtggc gtcgatctcg tagctgacct
      481 tgccgccggg ggtggccact tcgacgatgt cgccctcttc cttgccgatc agggcgcggg
      541 ccagcggcga ggagaccgag atcttgttgg ccttcacatc ggcctcgtcg tcgccgacga
      601 tgcggaaggt gagccgttcg tcggtggcgc tgttgatcag atcgacggtg gcgccaaaga
      661 tcaccttgcc gctgttggga atctgggtga tgtcgatgat ctgcgcctcg gagagcttgg
      721 attcgatctc gcggatgcgc ccttcggtga agctctgctg ctcgcgggcc gcatggtact
      781 cggcgttctc tttcaggtcg ccatgggcgc gcgcctcggc aatcgcattg atgatgcgcg
      841 gccgctccac cgtcttgagc cgttgcagct cctcttccaa ctgcttggcg ccctctttgg
      901 tcatcgggac ccttttcatc acttcagcct ctcgtgcagt gcctgcaatg tgtggacggc
      961 cagatcccct ttgaactgca acgcctggca gatggcctcg gcgcccgcca gggtggtggt
     1021 gtagtgaacc cggttgagca gtgcgtaacg gcgaatggtc gcggagtcgg caatggcctg
     1081 ccgtccttcg gtggtgttga cgatgaagga gatttcgccg tttttcaacc gatcgacgat
     1141 gtgcggccgc ccctcgccga ccttgttggt ctgctcgacc gccaaccctg ccgccttcag
     1201 cgccgctgcg gtgccccggg tggccgtcag cgagaagccg aggccgatca gtgcacgggc
     1261 cacctcgacc gcgccggcct tgtcggggtc gcgcacgctg agaaaggcga caccgccact
     1321 cggcagcagt tctccggcac cggcctgggc cttggcgaaa gcttcggcga acgtgaggcc
     1381 gacgcccatc acttcaccgg tcgatttcat ctccggtccc agaatcgggt cgacgccggg
     1441 gaacttgttg aacgggaaga ccgcctcctt gacgctgaca tagtcgggga tgcgttcggt
     1501 gacgaagccc tgctgcgcca ggtcgatgcc ggccatgcag cgggccgcca ccttggccag
     1561 cgagatgccg atgcatttgg agacgaaggg cacggtgcgc gaggcgcgcg ggttgacctc
     1621 gagcacatag atctcgccgt tctgcaccgc cagctgggtg ttcatcagcc cgaccacgcc
     1681 cagctccagc gccatcgcct cgatcatgcc gcgcatccgc tgctgcaatt cgggcgccag
     1741 actgtagggc ggcagcgcgc aggccgagtc gcccgagtgg acgccagcct gctcgatgtg
     1801 ctgcatgatg gcgccgatca ccacctgcct gccgtcactg accgcatcga tgtcgacctc
     1861 gaccgcatca tccagaaaac ggtcgagcag caccggccgc tcgctcgaag cctgcaccgc
     1921 ctcgaccatg tagcgcttca gttcggcggt gttgtagacg atctccatcg cccggccgcc
     1981 cagcacgtag gagggacgca ccaccagcgg atagccgatc gattcggccg ccaccagccc
     2041 ctcttcgagg ctgcgcacgg tgcggttggg cggttgcctg aggttgagct gctggatcat
     2101 ctgctggaac cgctcgcggt cctcggcccg atcgatcgcg tcgggcgtgg tgccgatgat
     2161 cggcacgccg gcgcgatgca gcgcccgcgc cagattgagt ggcgtctggc caccgaactg
     2221 gacgatcacc cccttgggct gctccagcgc gacgatctcg agcacatcct ccagcgtcac
     2281 cggctcgaag tagaggcggt cggaggtgtc gtagtcggtc gacaccgtct ctggattgca
     2341 gttgaccatg atggtctcgt agccatcctc gcgcatcgcc agcgccgcat gcacgcagca
     2401 gtagtcgaat tcgatgcctt ggccgatgcg gttgggtccg ccacccagca ccatgatctt
     2461 gtcacgctga ctgggtgccg cctcgcactc ctcgtcatag ctggagtaga ggtaggccgt
     2521 gctggcggcg aactccgcgg cgcaggtgtc gacccgcttg tagaccggcc gcacccccag
     2581 ccgatggcgc tgatcgcgca ctgcctgttc gtcgacgccg agcagtcgcg ccagccgggc
     2641 gtcggcaaag cccttgcgct tgagccgccg cagccagtcg gcgtcgagcg ccgccagcgg
     2701 ttggccgacc agcgcctgct cttcgcggat cagatcgtcg atgtaggcca ggaaccaggg
     2761 gtcgatggcg gtcagttcgt ggatctcatc gacccccatg ccggcacgga aggcatcacc
     2821 gatgtaccag agccgctcgg cagtcgggtt gatcagccgc tgccgcaact gctcctgcgc
     2881 atcccgggcg ccgaggtcga gcttcggatc gaaccccatc accccggttt cgaggccgcg
     2941 catggccttg tgcagcgact cctgaaagct gcggccaatc gccatcacct cgcccaccga
     3001 cttcatctgg gtggtgagga tgttgttggc ctcggcgaac ttctcgaagg tgaagcgcgg
     3061 aatcttggtg accacatagt cgatcgccgg ctcgaacgag gtcggaatca atccgccggt
     3121 gatctcgttg cgcagctcgt ccagcgtata gcccaccgcc agcttggcgg ccaccttggc
     3181 gatcgggaag ccggtcgcct tggaggccag cgccgaggag cgcgacaccc gcggattcat
     3241 ctcgatcacc accatgcggc cggtggcggg atcgacaccg aactgcacgt tggagccgcc
     3301 ggtctcgacg ccgatgcggc gcagcacccg gatggcggcg tcacgcagca gctgatactc
     3361 cttgtcagtc agtgtctgtg ccggtgccac cgtgatcgag tcgccggtgt gcacccccat
     3421 cgggtcgaag ttctcgatgg tgcagaccac gatgcagttg tcggcccgat cgcgcaccac
     3481 ctccagctcg aactccttcc agccgatcag cgactcgtcg atcagcagct cgcgggtcgg
     3541 cgacagcgcc agaccacgct ggcagatctc gatgaactcc tcgcggttgt aggcgatgcc
     3601 gccaccgctg ccgcccatcg tgaacgaggg acggatgatg cagggatagc cgatctgccc
     3661 ctgcacctgt tcggcctcct ccagcgaatg ggcaatcgcc gagcgcgggg tggccagccc
     3721 gatcgagcgc atcgcccggt cgaacagctg tcggtcttcg gcggtgtcga tcgcttcgcg
     3781 gttggcgccg atcatctcga cgccatactg cgccaggatg ccgtggcggt cgaggtcgag
     3841 cgcgcagttg agcgccgtct gaccgcccat ggtcggcagc agcgcgtcgg ggcgctcctg
     3901 ttcgatgatc tttgccaccg tgcgccagtt gatcggctcg atgtaggtgg catcggccat
     3961 caccgggtcg gtcatgatgg tggccggatt ggagttgacc aggatcaccc ggtaaccctc
     4021 ctcgcgcagc gccttgcagg cctgggcgcc ggagtagtcg aactcgcagg cctggccgat
     4081 cacgatcgga ccggcaccga gaatcagaat ggaaccaatg tctgtacgtt ttggcatggc
     4141 aacaggcgtc gtggtgcagc gtccggtcag ttgacctgac gctggcgatg aaggtcgatc
     4201 aggctgatga agtggtcgaa cagggcttcg gcatcatgcg gaccggggct tgcctcgggg
     4261 tggccctgaa agccgaaggc cagccggtca cgccgggcga agccctgcac gctgccatcg
     4321 aacagcgaca gatgggtcac ctcgatgtta tctggcaaat cctcggagtc gacggcaaaa
     4381 ccgtggttct ggctggtgat catcaccttg ccgctgcgca gatcctgcac cggatggttg
     4441 gcgccatggt ggccaaactt catcttcagc gttctggcac cgctggcgag cgccagcagc
     4501 tgatggccca ggcagatgcc gaacagcggc agatcgcgct gcagaaactc acgaatcgcc
     4561 gcgatggcgt agtcgcaggg ctccggatcg cccggtccgt tggagagaaa gacgccatcg
     4621 ggctgcaacg ccagcacctc ggtggccgag gtggtcgcgg gcaccaccgt cacccggcag
     4681 ccgcgctcgg ccagcatccg caggatgttg cgcttgacgc cgaaatcata ggccacgaca
     4741 tggtggcggc tctcggtgac cacgcggtgg ccggtcgaga tgcgccagca cccttcgctc
     4801 cagggatagg cgcgggcggt ggtcacctcc ttggccagat ccattccctg caaaccgggg
     4861 aaggcgcgcg ccgccgtcag cgcggccgac tgatcgagct cagcgccggc catgatgcag
     4921 ccgcgctgcg cgccttggtc gcgcaggatg cgcgtcagcc gccgggtgtc gaggccggcg
     4981 atgccgaccc ggcgatggtg acgcaggtac tcgtccagct caccctgacg gcgccagttg
     5041 ctgctgcgca gcggcagatc acgaatcacc agacccgccg catgaatgga cggggactct
     5101 tcatcttcgg cggtgatgcc ggtgttgccg atgtgagggt aggtgagggt gacgatctgc
     5161 cgggcatagg agggatcggt cagaatctcc tgatagccgc tcatcgaggt gttgaacacc
     5221 acctcgccga ctgccatgcc atcggcgccg atcgacatgc ccctgaaaca gcttccatct
     5281 tccagaatga gcacggctgg tctggctgac aagagcacac ccctttggtc tgtttgatgg
     5341 cagccggacg cacgcgcgcc cggccataaa aaagcgagag ggaacgatcg gttccatccc
     5401 gcttgcaccc agaagcgcca cgccccacgg agcggcttgt gacagatcga aaacggcgcc
     5461 attctatcca aaaatgccag gaagtgccac gccaacggcc gtcaacctgc cgcccgcgga
     5521 ttcgttatgg caagctaaaa acacgcactc ccattgcggc caatcgaggt tccgatgaac
     5581 gacatcccgg ccagcacgct tcccgcaccg atgccgatcg agccggccga cacatcggtc
     5641 aaggcagcac tttcgcactt ttttgaacag cacgagacca gtgcctacct gagggcgcgc
     5701 cgtgtcaccc gtgacagtga cacggcgctc gaactggtgc aggaggcgat gacccgtctg
     5761 gtgagccact accgtgatcg gccaccggca cagtggccgg cgctgtttca gcgcatcctg
     5821 cagaatcagt tgcgcgactg gcagcgacag cagtacggtc cgcgcgccgg ctggcgcttc
     5881 gccgcctacg atgagcagga tgaacagaac ccgaccctgc cagcggccag cagcctggag
     5941 ccggatcggg ccatggcccg tggccgtttt catgccgcgc tggcacaggc ggtggccagc
     6001 ctggcgcccc agcagcagcg caccttcatg ctgcgctgct gggatgggtt gaacgaacag
     6061 cagaccgccg atcgcctcgg tatcagcatc ggcagcgtca agacccacta ctttcgcgca
     6121 ctccagcagt tgcgcgcccg gctcgaagcc taccgccatg agtgatgaac aggagtcgat
     6181 tccagccgaa attcaccagc ggctgcgcca ggcccggctg caggcgatcg agaacggatt
     6241 gcggcagcgg ccgcgccggc tgccggagcg gttctggatg gcggcgacgg ccgtcatgct
     6301 ggcactgctg ctgacccacc gcccgccgcc gcaggatgac agtccgcagc agagcgccga
     6361 actgctggcc gaggtcgagt tctaccaatg gctcggtctg acggccgaag ccggctgaat
     6421 cctgaaagtt cccttaccgc aacaatcacc agccgacctc atcactgtca caaacagcgg
     6481 cctgtcaccg tcacaagaag agggaagcgg gtctgatgtt tgtcattttt ctgtcatatg
     6541 cacctcttag gctttgcgcc tgtttcgatc cggaacagtc agggtggcct gctgccatcc
     6601 ccaatccaac ggagaccact catgcgtaaa ctgcttctgc ccgcactgat cagcgctgcc
     6661 agcctctcca tcgccacctc ggcgctggcc ggcaccgtga ccaccgacgg ctctgacatc
     6721 atcatcaaga ccaagggcgg actcgaagcc aagaccgccg acggcaaggc ctcgttcaag
     6781 ctcggcggcc gcgttcagct cgattacaac ggctacgatg gcgtcatcaa cacgaaaacc
     6841 cctggcgaga atggcagtga tcttttcttc cgccgtgccc gtctggagat gggcggcacc
     6901 tacaaggatt ggggctacct gatgtcctac aacctgaccg aaagcggcag catcgatctg
     6961 ctgtggacca gctacaacgg tttcggcaag ctggccgagc tgacctttgg ccagcagaaa
     7021 gaggatttcg gcctcgaaga cactggcagc tccaaatgga tcaccgccat cgaacgctcg
     7081 atgccgagca atgccttcga caccaagaac aatgtcggcg tcaaactgca cggcgccaac
     7141 aagaccttca gctacagcct gggcgcctac aagcaggacc tcgacaacag caacgacctc
     7201 aacagcgccc tgaccggccg cttcgtcgtg cgaccgatct atggcaagga ccgtctgctg
     7261 cacctgggtg ttggcgccac ctcgcgtgat ggcaagttca ccaagatcag ctcccggctg
     7321 ggcgtgcgtg gtaacgaaga tggcagcgcg gcgcaagtgg tcagtgcggc ctacaccggc
     7381 actccggctc cagtggcgga tgggcaggat gtctggaacg ccgagctggc cggctacctg
     7441 ggtccggtcc acctgatggc cgaatatttc gacggcaaga tctctggtgt gaacagcagc
     7501 cccgacatcg aagccaacgg ctactacgtg caactgggct ggatcgtgac tggcgaacac
     7561 cgccgctaca acgatgagat cgcggccttc gacaaggtca aaccggtcaa cccgcagggc
     7621 gcctgggaga tcttcgcccg ctatgacgag ctggatgcct cggacaacca gaatgtcgca
     7681 gtgaagatgg tcggcgaaac cggcaacacc accaccctcg gtgtcaactg gtacgccaat
     7741 gacctggtca aggtggggct gaactatgtg cacgccaaga ccgatcagcc gattggcggc
     7801 gaggacagcg gcgacgcact ggtcagccgg ttgcagatcg ccttctgatc aaaaccgcat
     7861 tgccaacaaa aaagccccgg tgtggggctt ttttgtttct ggtctgtttc tggcgatccg
     7921 ccagcgaggc ggatcgctga atgccagcgg cgctactcga tctcctcgac gccgccttcg
     7981 gcgctgatcg gttcgctgcc atattcgacc ttcggctttt tcgccggctg ccgcagcgtg
     8041 ttctcgacca catcgatggt gccgccgcgc ttcaggaact gctcgacatc gctctgcatc
     8101 ttctgccgca gccgcgcccg cgaggacatg gtccgcgcct cggcggattc atccttgctg
     8161 ccgatgtagt catagtcgaa atcacctcgg ttcacgccat cgagcagatc gccaccacca
     8221 ctcttcttgg ccatcgatta cacctcttct tccgtctcaa catgctgata accatagcca
     8281 atcctgcctg accgattggt cgggatggcc aactctacag gattttattt ctgtcacaaa
     8341 aaacttttgt gacccgcctc acagggcagc gccgtcatgg cgcggatgga ccctgcggca
     8401 tgaagcggcg gcagtttaga gaaagtatca gctgtcaaca agcaggcacg gggagtgccg
     8461 tgcatcgcac ggtggggcgg ccgggtgggc tcaggtctgc gcgccgagga tgcgggccag
     8521 aaacagccgg aacagctgtt cgaggtcggt cagtcgatcg accagatcat ggccgctcag
     8581 cagcagatgc aggtcgcact cgacctcgct ggcgaagcgc agcgcggccg ccaccggtac
     8641 gatctcatcc cgccagccat ggatgacgac ccgctgccgg gtgcgcggca cgaagtggct
     8701 ctgcggatag tcgggacggt cgatgtagag cgccggcgcc agcaggaaca ggccggcaac
     8761 ctcacgcagg ttggcggtgg ctgcggccac gtagccgccc atgctggagc cgaccagaat
     8821 cggctggcgt gactcgtcgt tcagcagcgc cagcagcttg tcgacccgcg ccggaccgga
     8881 gagtccctgg tagtcgggac tctcgaccgc cagccccagc tcccgcgcca cctgggccag
     8941 cgcggtgatc ttgatgcccc agggaccgct ctccttgccg tgggcaaagt agacgacatg
     9001 ctcgctcatg gcccgctccc gatcagttga cgaagctgtg ccactcggga tggccatcgc
     9061 gtcgggcggc cacttcgtcg aagtagagct gctgcaggcg ggtggtgatc gggccacgcg
     9121 tgccgctgcc gatgatgcgg ccatcgagcg actggatcgg cgtcacctcg gcggcggtgc
     9181 cggtgaagaa ggcctcgtcg gccacataga cctcgtcacg ggtgatgcgc ttctcgacga
     9241 ttttcagccc ctcttcttcg gccagcgtgc agacggtgcg gcgggtgatg ccgtcgagac
     9301 aggaggtgag ctccggcgtg tagagcaccc cgcgcgacac catgaagaag ttctcgcccg
     9361 acccctcgga gacatagcct tcgctgtcga gcagcagtgc ctcgtcggcg ccgctgccga
     9421 gtgcctcctg cagcgccagc atcgagttga tgtagttgcc attggccttg gccttgcaca
     9481 tcgcgatgtt gacgtggttg cgggtgtagg aggaggtgcg caccttgatg ccattgacca
     9541 gcgcctcggg gctcatgtag ctcgaccaga cccaggcggc gatcatcaca tgcaccttga
     9601 ggttgtcggc acgcagcccc atgccttcgg cgccatagaa ggccatcggc cggatgtagg
     9661 cctcgggcag atcgttgacg cgaatcagct ccagctgggc tgcgttgagc tgctgctggg
     9721 tgtaggggat cttcatgttc atgatgtgtg ccgagcggaa cagccgttcg gtgtggtcga
     9781 ccagccggaa gatgcagggg ccacggtcgg tgcggtaggc gcgggtgccc tcgaagaccc
     9841 ccatgccgta gtgcagggtg tgggtcagca catgaacccg ggcatcgcgc cagggcacca
     9901 tctcgccatc cagccagatc aggccatcac ggtcggccat ggtcgctgta gtcatcatcg
     9961 atcctctgct ttgtgcggtt gtctgttgtc ggattgcgcg gatcacgctg cgggcggcac
    10021 cagcagccgc tgccagatcg cagccacctg ccggcgcgcg tcgcgccaag gcgcggccgg
    10081 caccacctgg ggctctttca tcagtgccaa tcggtgcgtg ctggtgcgat agctgcggta
    10141 gtggtcgcgc agttgccgtg cttcgtcggc gccgagcagc gcggcctctt ccaggccatc
    10201 gagaatgcga atcgtgtcgg tgaattgtaa cagttgcgga tggtcggcgg accacgccag
    10261 cacgccatat tgggccagaa actcgatgtc gacgatgccg ccgggatcct gcttgaggtc
    10321 gaacagcgcg gcctcgtcga tcgcctgatg ccgatcgacg ctgcccagct cctcgaccat
    10381 gcgcccccgc atcttcgcca cctcgtcgcg cagcgtcggc agatcgcgcg gccggccgag
    10441 cacctcggcg cgcacctgct cgaacttctg ccgcagtcga tggcagcccg ccaccacccg
    10501 cgcgcgcacc agtgcctgat gctcccaggt ccaggcatct tcgagctggt agccacgaaa
    10561 ggcgttgagc gagctgacca gcaccccgga acgcccggac gggcgcagcc ggatgtccac
    10621 ctcatagagc tgccccgacg gcgtcaccgc ggtgaggatg tgcatcatcc gcgccaccag
    10681 ccgcgagaag aacaggccgt tgtcgatcgg ctggtcgcca tcggtggtga ggttgggcgc
    10741 cgcgtcatgg atgaagacca gatcgaggtc ggagctgtag gagagctcga tgccgccgag
    10801 cttgccatag ccgacgatga tgaagtcggg gttgcaggcc acccctggcg cccgctgcgg
    10861 caccccatgc ttgcgggtca ggtggcgcca ggccaggttg aacacctgca ccagcaccgc
    10921 ctcggcgatg aaggtgaggt agtcgctcgc cttcatcagc gggatgcgct gcgccatttc
    10981 ggcggccgcc accttcagca gatgggcctg cttgaagtag cgcagcccct ccatctgcgc
    11041 ctccaggtca tcctcgggaa tgcgcagcag tcgcaggttg agcgcctcct gcaccgcctg
    11101 tcgctcgggt ggtgcgtaga ggttctgcgc gtcgagaaac tcgtcgagca ggcttggaaa
    11161 gcggctgatc agatcggtga tccaggggct gagtgcgcag aggtcgatca gatgatgcag
    11221 cgccgagggg ttctcggtca gcagcaccag atagaccgtg cggcgcagca gcgcctcgat
    11281 caccgtcagc acccgctcca gcgcgacctg cggtgtggcg tggcgggcga tgtcggccag
    11341 cagcatcggc agcagccggt cgagccgtgc ccgcgcctca tcccgcagtg ccgcgacccg
    11401 cggctcactg cgcaactgcg cgatgcgctg catcagtgcc tgcggctgct gcagaccctg
    11461 ctcggtcagc cagcgggcgg cctgatcctc cgccagctcg ccgcgccaca gcttcagcca
    11521 gccgctgccc tcgggcgtcg gcagcgcgct tttcgcctcc ggttcgctga ccatctcgtt
    11581 gaagtgacgg tggaccgtct cgcggtactc ggtcagtcgc tgctgaaaac ccgaccagtc
    11641 ggcaaagccc atcgcaaagg ccacccgttc ccgctcggcc tcgtcggtgg gcaggttctg
    11701 cgtctgccgg tcatccagct cctgcagcac atgctcgctg tcgcgcaaaa agaggtaggc
    11761 ctcgcgcagc gcggccacgg tcggttgcgg cagatagcca ccatcggcaa tctgcccgag
    11821 cgccgccagc aggctggtct ggcgcaggcg cggatcacgg ccaccgtgac tcaactgcaa
    11881 cgcctggacg atgaactcca cctcgcggat gccgcccgaa ccgagcttga ggttctgctc
    11941 cagccccttt tcgcgcacct cgcgctgaat catcgccttc agctcgcgca gcgcgccgat
    12001 cacactgaag tcgagatagc ggcggtagac gaagggggtc agccgtgcca tcagatcggc
    12061 cgcggccgca ggcgcgccgg cgatcacccg tgccttcagc atcgcatagc gctcccagac
    12121 ccggccctgc tcctggtagt aactctccat cgcatcgaag ctcggcacca gcgcaccgct
    12181 ctgcccatag ggtcgcagcc gcatgtcgac gcgaaagaca ctcccctcgt cggtggcgcg
    12241 gtcgagcacg ttgatcagcc gctgcccgag ccgggtgaaa aaacggccgt tgtcgatcga
    12301 ctgcggtccg ccccgggtct cgcccgcctc ggagcaggcg aagatcaggt cgatgtcgga
    12361 agagaaattg agctcgccgc cacccagctt gcccatgccg agcaccacca tcaccgcctc
    12421 gcggccggtg tggcggccgg tcggcacgcc atgactgcgg caaccatcct gatacagcca
    12481 accgagggcg acatcgactg cggcttcggc cagtgccgag agctcgcggc tggtggtgac
    12541 gaaatccgcc agccggcatc ggtcacggca ggcgatccgc accatctcgc ggcgacgcca
    12601 gcggcgcagc tgctgcatca gtgccgcctc gctgtcgacc acagtgagca gttgcttcgc
    12661 ctgcgcttga tacattccgg acggatagtc acggtcgagg tcgccgcttg cctccagctc
    12721 cagcgcgagc attggctggg tcacgcagca gtcggcaacg aaatcgctca ccgcccacac
    12781 ccgcagcagc cgttcgagcc gcgcgggcgg cagcgccaga tagcgctgca tcgtcgcgtc
    12841 atcgagccgc tgccagtggc gcagcaccac cgcctgcagc gcggcgggca gaaaatcgat
    12901 cggcggattc atcggcacag caccactaca ctttcaggct gaaggggttg aaatcggtct
    12961 tgtcatcgaa gtaatcctca tgctcggccc gcttcaatgc cgccacgatg cggtaggtca
    13021 gcggcgtcat caccacctcc cagcccacct tgatgcagta gttcattgcc atcaccgcca
    13081 gcagcagatc ggtctcccag acgccataga aggccagcgg atagaacacc agcgtgtcga
    13141 ccgcctcacc ggcgacggtc gagccgatcg tgcggctcca gaggtgacgc ccctgggtca
    13201 gcaccttcat tcgcgccagt gtgaaggagt tggtgaactc gccgcaccag taaccgatca
    13261 gcgaggccag cgcgatgcgc caagtggagc cgaaggccgc ctcgatcacc gcctgcccct
    13321 cccagccggc ggccggcggc atattcacca ccaccgtgct catgaagctg gcgaaggcca
    13381 gcgcgccaaa accggcccac accacccgcc gcgagcgggc gaagccgtac acctcggtca
    13441 ggatgtcgcc gaacaggtaa ctgatcggaa agaacaggtt gccagcgctg aaggtgaagc
    13501 cggccaccgt ggtcaccttg ctgacgccga tcaggttggc gcagagcagc accgtgacga
    13561 aggccgccat gaccaggtca tagtaacggt agtgacggcg gtggcgcggc gcggtcatgc
    13621 ggggctccag catgcaagtc ggacgggtga atcggtggct gtcgcattgc gcggcagttc
    13681 agcctgccgt ctgccgccgc cggcgtcaac acgcggccgt ggcagctcct tctggctgca
    13741 ttatcgatcc agccatgccc tgatgcacaa tggaacaccc caaccgcacc gacgaggttg
    13801 tctcccatgc accacgccgc caccctgcac agtccgctcg gcgaactgac cctctgcgcc
    13861 agcgaccacg ccttgaccgc gatccgcttt cctgaagagc cgccaccggc cctgcccgtg
    13921 gccggcccca atgccctgct gcgcgaggcc atccgccagc tcactgccta cttcgacggc
    13981 cgcctgcagc agttcgacct gccgcttgcc ggcgagggca gcccatttca gcagcgggtg
    14041 tggcaagcac tgctgcaggt tccccatggc cgcaccgcca gctatggcgc catcgccgcg
    14101 gccatcggca atcctcgcgc ctgccgcgcc gtgggactgg ccaacggcag aaacccgatc
    14161 gccatcgtgg tgccctgcca ccgcatcatc ggccgcaacg gtcaactcac cggctatggc
    14221 ggcggtctgg agcgcaaacg gtggctgctg cggcacgagg gggtgggggt gaattgaagg
    14281 atctggggca cactatggct gcgccgaaat cgagcgaatg ccgcattgcg gacaatcatc
    14341 ctggcttcgc tgctgaaaag tgttcaatgg ctgtcagtgg ttttggcctg ttctcccatc
    14401 aggcaggccg gtgacttgtc cggtctggag agcaggaaac cgagaaaatc gtggtctgag
    14461 ctcaattacc ccccccagcg gttcatcccc gcgggtgcgg ggaacgc
//
LOCUS       DAORCQ010000061        14286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2736_length_14286_cov_6.581337, whole genome shotgun sequence.
ACCESSION   DAORCQ010000061 DAORCQ010000000
VERSION     DAORCQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14286
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_14286_cov_6.581337"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKI23_07645"
     CDS             <1..303
                     /locus_tag="PKI23_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNN36848.1"
                     /translation="AQTQGVEPGLPLPVVITVYSDKSFTFITKTPPATVLLKKAAGIK
                     SGSKVPNKNKVGKVTRQQVEEIAKQKMPDLTAATLEAAVRTIAGSARSAGIEVEGL"
     gene            305..1000
                     /gene="rplA"
                     /locus_tag="PKI23_07650"
     CDS             305..1000
                     /gene="rplA"
                     /locus_tag="PKI23_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNN36849.1"
                     /translation="MAKLSKRAQQIRARIEPGKLYSVEEAVALLTDLSKVKFVESFDV
                     SVNLGVDPRKSDQVVRGATVLPNGTGKEVRVAVFASGEKADQALAAGADVVGMEDLAE
                     RIKGGDLDFGVVIASPDAMRVVGQLGQILGPRGLMPNPKVGTVAVDVAQAVRNAKSGQ
                     VRYRTDKAGIIHGSIGRIGFPADAIKGNLLALLSDLKRAKPSTSKGVYMRKVSLSTTM
                     GPSLTIDQSSLEL"
     gene            1299..1829
                     /gene="rplJ"
                     /locus_tag="PKI23_07655"
     CDS             1299..1829
                     /gene="rplJ"
                     /locus_tag="PKI23_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNN36850.1"
                     /translation="MALRLEDKKAIIAEVNEVAGRALSVVIADYRGVTVGDITGLRVK
                     ARDSGVYLRVVRNTLASRAVEGTEFQCLQQALVGPTILAFSMEDPGAAARLLKEFAKQ
                     NDRFVIKALAVGGKLMGAEQIDALATLPTREEALSRLMSVMQGPIAKLARTINEVPSK
                     LVRTLAAVNDQKREAA"
     gene            1897..2274
                     /gene="rplL"
                     /locus_tag="PKI23_07660"
     CDS             1897..2274
                     /gene="rplL"
                     /locus_tag="PKI23_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNN36851.1"
                     /translation="MALNKDEILDAIASMTVMEVVDLIKAMEEKFGVTAAAAVAVAAP
                     AAAGEAAAAEEQTEFNVILKDSGEKKVNVIKVVRSLTNLGLKEAKDLVDGAPATVKEG
                     VSKAEAEDIKKQLEEAGATVEVK"
     gene            2533..6678
                     /gene="rpoB"
                     /locus_tag="PKI23_07665"
     CDS             2533..6678
                     /gene="rpoB"
                     /locus_tag="PKI23_07665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNN36852.1"
                     /translation="MAYSYTEKKRIRKAFGKLPQVMEVPYLLAIQLDSYREFLQIDKD
                     PLARMDIGLHAAFKSVFPIVSYSGNAALEYVSYALGEPPFDVGECQLRGVTYAAPLRV
                     RVRLILYDRESSNKVIKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVF
                     FDHDKGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDILFVRIDRRRKLYATILLRALG
                     YSTEEILALFFDNDVLHLQDDKYVLDLIPGRLRGESASFDIVDGKGNVLVEKGRRITA
                     RHIRAMEKALLKQIEVPEEYVLGHVTAKDLIDTETGEVICPCNSVITEELLQLIKAKG
                     IASVELLYTNELNCGAFISETLRLDQTRNAFDARVEIYRMMRPGEPPTKESADALFHN
                     LFFSQERYDLSAVGRMKFGRRVGSKIDESVGILYDGKYCAGRKNDDLTEMLHEEYGSE
                     ISDIVDVMRTLIAIRNGFGVVDDIDHLGNRRVRSVGEMAENQFRVGLVRVERAVKERL
                     GMAETEGLMPQDLINAKPVAAAVKEFFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGG
                     LTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLATYARANSYGFLESPYRRVV
                     DGRITDQIDYLSAIEEAEFVIAQASAEVDVEGRLIDELVAVRHQNEFTVMAPEQVQYM
                     DVSPQQVVSIAASLIPFLEHDDANRALMGSNMQRQAVPTLRAEKPVVGTGMERTVAED
                     SGVCVVARRGGVIESVDAARIVVRASDAETEAGDAGVDIYNLTKYTRSNQNTYINQRP
                     IVQKGDRIERGDILADGPSIDKGELALGQNMRVAFMPWNGYNFEDSILISERVVQEDR
                     FTTIHIQELTCVARDTKLGPEEITADIPNVGESALGKLDEAGVVFIGAEVNAGDILVG
                     KVTPKGEAQLTPEEKLLRAIFGEKASDVKDTSLRVPSSTKGTVIDVQIFTRDGVEKDS
                     RALQIEQMQLNEIRKDLKEEQRIIRDASYERLRAALVGQPVVRAPGLKKGDPIQPDYL
                     DQLAAADWFRIRMSDELLNEQLEQTEARLKEHQQELDRKFEDKRRKLTSGADLAPGVL
                     KIVKVYLAIKRQAQPGDKMAGRHGNKGVISVIMPVEDMPYDQNGEPIDIVLNPLGVPS
                     RMNVGQVLETHLGWAAKGLGRKIGELLERKASIDELRSFLDQIYNRVGGRKERLDELT
                     EAELIELSENLTAGVPMATPVFDGASEDEIKSMLRLAGLPESGQTLLYDGRTGEAFDR
                     PVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALKAY
                     GAAYTLQEMLTVKSDDVNGRTRIYKNIVDGDYRMDPGMPESFNVLVKEIRSLGINIEL
                     ENQ"
     gene            6746..10957
                     /gene="rpoC"
                     /locus_tag="PKI23_07670"
     CDS             6746..10957
                     /gene="rpoC"
                     /locus_tag="PKI23_07670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNN36853.1"
                     /translation="MKDLLSLLKTQTQTEEFDFIRIGLASPEMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVALTKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFESFVVLDPGMTTLEKGQML
                     SDEQYYEALEEFGDEFTAKMGAEAIQELLKEIDLEAEITRLRAEIPETSSETKLKKYS
                     KRLKLLEGLLGSGDRPEWMVLKVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLNAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSTKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLRLHQCGLPKKMAIELFKPFIFGKLEHSGLAT
                     TIKAAKKMVERETPEVWDILAEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHIPLTLEAQLEARSLMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRERINARGEGMCFSDVEEVDRAYHCGQVDLHAKIKVRLDEMLINAEGAAARS
                     ALIDTTVGRTLLSKILPNGVPFDLINRPMTKKAISRIINACYRNVGLKETVIFADQLM
                     YTGFFYSTKSGVSIGIDDLVIPQAKARFIDQAEEEVKEIEAQYASGLVTQGEKYNKVI
                     DIWSRTNDLVAKSMMATLGSEQVRDRDGNEVQQESFNSIYMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLSVLQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVAQDLVVTEVDCGTRNGVLMAPVIEGGDVIEALGERILGRVLAEDVINRTTEEVA
                     IPAGTLIDEQWIQRIEELGIDEVQVRSAITCETRHGICSSCYGRDLARGHVVNVGEAI
                     GVVAAQSIGEPGTQLTMRTFHIGGAASRASATDSIQVKHGGKIRLHNLKFVEQHNGNL
                     VAVSRSGELAIADQDGRERERYKVPYGAVLTVRDEQVVEAGTVVAKWDPHTHPIITEV
                     AGTVRFVGMEEGISIRHQTDEVTGLSSISVMDPSERPAAGKDLRPAIMLLDEQGNEIL
                     LAGSNLPAHYLLPAKAIVSLAANARVDVGDVLARIPQEGLKTRDITGGLPRVADLFEA
                     RKPKESAILAEISGVVSFGRETKGKRRLMITPADGVNPIDGSSHYEELIPKWRTLSVF
                     EGEFVEKGEVVSEGATNPHDILRLKGVNELAKYIVSEIQDVYRLQGVKINDKHIEVII
                     RQMLRKVDIVDAGDSSFIKGEQSEYALVLEENDRLVAEGKTPALFERVLLGITKASLA
                     TESFISAASFQETTRVLTEAAVTGKQDQLRGLKENVVVGRLIPAGTGLSYHSEARRRR
                     RRGEMESTSTASEVEQALSEELKSI"
     gene            11134..11508
                     /gene="rpsL"
                     /locus_tag="PKI23_07675"
     CDS             11134..11508
                     /gene="rpsL"
                     /locus_tag="PKI23_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNN36854.1"
                     /translation="MATINQLVRKPRKRRVVKSDVPALQSCPQRRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTSGVKDRRQARSKYGTKKPKS"
     gene            11590..12060
                     /gene="rpsG"
                     /locus_tag="PKI23_07680"
     CDS             11590..12060
                     /gene="rpsG"
                     /locus_tag="PKI23_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNN36855.1"
                     /translation="MPRRRVIAKREILPDAKYGSPLVARFMNHVMEDGKKSIAERIVY
                     GALDIVAERTKGDPLKIFESALDAVAPLVEVKSRRVGGATYQVPVEVRASRRGALSMR
                     WLVQYSRARSEKSMPQRLAGEIMDAAQGRGAAVKKREDVHRMAEANKAFSHYRF"
     gene            12100..14196
                     /gene="fusA"
                     /locus_tag="PKI23_07685"
     CDS             12100..14196
                     /gene="fusA"
                     /locus_tag="PKI23_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNN36856.1"
                     /translation="MARTTPLKRYRNIGICAHVDAGKTTTTERVLLYTGVSHKLGEVH
                     EGTATMDWMAQEQERGITITSAATTCFWKGMDQQFDQHRINIIDTPGHVDFTIEVERS
                     LRVLDGAVVVFCATAGVEPQSETVWRQANKYGVPRIVFVNKMDRAGADFLRVVDQIKN
                     RLGANPVPIQLPIGSEENFEGVVDLLRMKAIYWNEADAGMTYEARPISAEMKEICDSY
                     RELLIESAAEASDELMEKYLEGSELTTDEIRQALRARTIRGEIVPALCGSAFKNKGVQ
                     AMLDAVIEYLPAPDEVKAISGMLEDGETVIERKSSDDEPFAALAFKIATDPYVGTLTF
                     FRVYSGVLSSGDAIYNSVKGKRERVGRMVQMHANDRTEIKEVRAGDIAAAVGLKDVTT
                     GDTLCDLNKPVVLERMEFPEPVISVAVEPKSKPDQEKMGVALGKLAQEDPSFRVKTDE
                     ESGQTIISGMGELHLDIIVDRMRREFGVEANIGKPQVAYRETIRKKVEVEGKFVRQSG
                     GRGQYGHVWLRLEPLPEGEEYQFVNEVVGGTVPREYIPAVDKGVREQMQNGILAGYPL
                     LALKATIFDGSYHDVDSNEMAFKVAGSMALKKGALEADPVLLEPIMKVEVVTPEDYMG
                     DVVGDLNRRRGMIQGMDDSPSGKVVTAEVPLAEMFGYSTDLRSATQGRATYSMEFKRY
                     AETPNNIAEAIISKNK"
BASE COUNT         2984 a         4059 c         4637 g         2606 t
ORIGIN      
        1 gcccagaccc agggtgtcga gccgggtctg ccgctgccgg tggtgatcac ggtctactcc
       61 gacaaaagct tcaccttcat caccaagacc ccgcctgcga ccgtgctgct gaagaaagcg
      121 gcaggcatca agagtggcag caaggtgccc aacaagaaca aggtgggcaa ggtcacccgg
      181 cagcaggtcg aagagatcgc caagcagaaa atgcccgatc tgacggcggc aacgctggag
      241 gcggccgtgc gcaccattgc cggcagcgcg cgcagtgccg gcatcgaagt ggaggggctg
      301 tgacatggcg aaattgagca aacgggcgca gcagatccgc gctcgcatcg agccgggcaa
      361 actctacagc gtcgaagagg cggtggcgtt gttgaccgat ctctccaagg tcaagttcgt
      421 cgagtcattc gatgtctcag tcaaccttgg agttgatccg cgcaaatccg atcaggtggt
      481 gcggggcgcc accgtgctgc ccaacggcac aggcaaggag gtgcgggttg ctgtctttgc
      541 gtcaggcgag aaggcagacc aagcgctggc ggccggtgcc gatgtcgtcg gcatggagga
      601 tctggccgag cgcatcaagg gcggtgatct cgactttggt gtggtgatcg cctcgcccga
      661 tgcgatgcgt gtggtgggcc agcttggtca gattctgggt ccgcgtggcc tgatgccgaa
      721 ccccaaggtc ggcaccgtgg cggtggacgt ggcgcaggcg gtcagaaacg cgaagtcggg
      781 tcaggtgcgc taccggaccg acaaggccgg gatcatccat ggctcgatcg gccggatcgg
      841 ttttccggct gacgcgatca aggggaatct gctcgcgctg ctctccgatc tgaaacgagc
      901 caagccgtcg acctccaaag gggtgtacat gcgcaaggtg agtctcagca ccaccatggg
      961 gccaagcctg acgatcgatc agtccagcct cgagctgtga cccctccaaa aaccgaattg
     1021 gtcagagtcc gaatctgacg aaaagacttt gcacttgccg gcgaggcctc tttggcgatc
     1081 accgggagct gtcagagacc ataggtcctt tatgtgtggg taaccacgca gtcgaaaggt
     1141 taatgttgcc tatgtaggcg gtgcgactga gacctcagtc tctcccgcgt tgttgtaaag
     1201 aggcgaaccc ttgatcttca aggctcggag ggttcgctgg cgtgctctcg accgatggtc
     1261 gttgatcatc gaccgagggt taattctgga gtaaaccgat ggcattaaga ctcgaagaca
     1321 aaaaggcgat catcgctgaa gtcaatgagg tggccggacg tgctctgtct gtggtcattg
     1381 ctgattaccg tggcgtcact gtgggcgata tcaccggatt gcgcgtgaag gcgcgtgatt
     1441 cgggtgtcta tctgcgggtc gtgcgcaaca ccctggcaag tcgagcagtg gaaggaaccg
     1501 aatttcagtg tctgcagcag gcgctggtcg gtccgaccat tctcgcgttc tccatggagg
     1561 acccaggtgc tgcggcgcgg ctgctgaaag agttcgcaaa gcagaacgac cgctttgtca
     1621 tcaaggcatt ggccgtgggc ggaaaattga tgggcgccga gcagatcgat gctctggcga
     1681 cgctgccgac ccgtgaggag gcgctgtctc gcctcatgag cgtcatgcag ggtccgattg
     1741 ccaagctggc tcgcaccatc aacgaagtgc cttccaaact ggtacgcacc ctggccgctg
     1801 tcaacgatca aaagagagag gcggcatgac gctcgacctt cgagcgtcat gccggcttaa
     1861 gacgaacagc aaacgaatag aagtggagaa acgtaaatgg ctctgaacaa agacgagatc
     1921 cttgacgcaa ttgcttccat gactgtcatg gaagtggtcg atctgatcaa ggcgatggaa
     1981 gagaaatttg gtgtcaccgc cgccgccgcg gtggccgtgg ccgcaccggc agccgctggc
     2041 gaagctgccg ccgccgagga gcagaccgag ttcaacgtga ttctgaaaga ttccggcgag
     2101 aagaaggtca acgtgatcaa ggtggtccgt tcgctgacca acctgggtct caaggaggcc
     2161 aaggatctgg tggacggcgc gccggcgacg gtcaaggagg gtgtttccaa agccgaagcc
     2221 gaggacatca agaagcaact cgaagaggct ggcgcgacgg tggaggtcaa gtaatcctct
     2281 tgtccaacac gctaaaaagc agcgcaaggc tggtggcatt ccgccatcgg cctttcgttg
     2341 ttgtacagaa ttcagttccg gatcagtttt agttttgaag agcggtgcgc acctttgcac
     2401 cagtgtgcac ggtgcaagga ttgcagcgat cggttcgctg atcggcgcaa ccaccgtcag
     2461 tcgatttcgt attgatgccc gatgtgattg aaactgtctc gataacccgt tgaatgaggc
     2521 tcggataggc gaatggcata ctcctacact gaaaaaaagc ggattcgcaa ggcgttcggc
     2581 aagctgccgc aggtcatgga agtcccttat cttctggcga ttcagctcga ctcctaccgg
     2641 gagtttctgc agatcgacaa agatccgctg gcacgtatgg acatcggtct gcacgccgcc
     2701 ttcaagtcag tttttccgat cgtcagctat tcgggcaatg ccgcactcga atatgtctca
     2761 tatgcgctcg gcgagccgcc ctttgacgtc ggcgagtgtc aactgcgtgg tgtgacctac
     2821 gcagcgccgt tgcgggtgcg ggtgcggctg attctgtatg accgggagtc ttcgaacaag
     2881 gtcatcaagg acatcaagga gcaagaggtc tacatgggcg agattccgct catgaccgac
     2941 aacggcacct tcgtcatcaa tggcaccgag cgggtcatcg tctcgcagtt gcaccgttcg
     3001 cccggggtgt tcttcgacca tgacaagggc aagacccact cgtcgggcaa gctgctctac
     3061 tctgcccgca tcatcccgta ccgcggttca tggctcgact tcgagttcga tccgaaagac
     3121 atcctcttcg tgcgcatcga ccgccgccgc aagctctatg ccaccattct gctgcgggcg
     3181 ctgggttaca gcaccgagga gattcttgcg ctcttcttcg acaacgatgt gcttcatctg
     3241 caggacgaca agtatgtgct ggatctgatt ccagggcgtc tgcgtggcga gtcggccagt
     3301 ttcgacatcg tcgacggcaa gggcaatgtg ctggtcgaga aggggcggcg gatcaccgca
     3361 cgccacatcc gtgcgatgga gaaggccctc ctcaagcaga tcgaggtgcc ggaagagtat
     3421 gtgctgggcc atgtcaccgc caaggatctg atcgacaccg agactggcga agtgatctgt
     3481 ccctgcaaca gcgtgatcac cgaagagctg ctgcaactga tcaaggccaa gggcatcgcc
     3541 agcgtcgaac tgctctatac caatgagctc aactgcgggg ccttcatctc cgaaacattg
     3601 cgtctggatc agacccgcaa cgcgttcgat gcgcgggtcg agatctaccg gatgatgcgg
     3661 ccgggcgagc cgccgaccaa ggagtcggcc gatgcactgt tccacaatct gttcttttcg
     3721 caggagcgct acgacctgtc ggcagtcggc cgcatgaagt tcggccgccg ggtcggcagc
     3781 aagatcgacg agtcggtcgg catcctctat gacggcaaat attgcgccgg acgcaagaac
     3841 gacgacttga ccgaaatgct gcatgaggag tacggcagcg aaatttcaga catcgttgac
     3901 gtgatgcgca cgctgatcgc catccgcaac ggtttcggcg tggtcgatga catcgatcac
     3961 ctcggcaacc gtcgggtgcg ctcggtcggc gagatggccg agaaccagtt ccgtgtcggc
     4021 ctggtcaggg tcgagcgcgc cgtcaaggag cggctcggca tggccgagac cgaaggcctg
     4081 atgccgcagg atctgatcaa tgccaaacca gtggcggccg cagtcaagga gttctttgga
     4141 tcgagccagc tctcccagtt catggaccag aacaatcccc tctccgaggt gacgcacaaa
     4201 cggcgggtgt cggccctggg gccgggtgga ttgacccggg agcgggccgg tttcgaggtg
     4261 cgcgacgtgc atccgaccca ctatggtcgg gtctgcccga tcgagacgcc ggaggggccg
     4321 aacatcggcc tgatcaactc gctggcgacc tatgcccgcg ccaactccta cggttttctc
     4381 gaaagcccct atcggcgggt ggtcgatggt cggatcaccg atcagatcga ctacctttcg
     4441 gccatcgaag aggccgaatt cgtcatcgcg caggccagcg ccgaggtcga cgtcgagggc
     4501 cgtttgatcg atgaactggt ggcggtacgg catcagaacg aattcaccgt gatggcgccg
     4561 gagcaggtgc agtacatgga tgtctcgccg cagcaggtgg tgtcgatcgc ggcctcgctg
     4621 attccctttc tcgagcatga cgatgcgaac cgcgcgttga tgggctcgaa catgcagcgt
     4681 caggcggtgc cgacgctgcg tgccgaaaaa ccggtggtcg gcaccggcat ggagcgcacc
     4741 gtggccgagg acagcggggt ctgtgtcgtc gcccggcgtg ggggtgtcat cgagagcgtc
     4801 gatgcggccc gcatcgtggt gcgcgccagc gatgccgaga ccgaagcggg cgatgccggg
     4861 gtcgacatct acaacctgac caagtacacc cgttcgaacc agaacaccta catcaaccag
     4921 cggccgattg tccagaaggg cgatcggatc gagcgcggcg acatcctggc cgatggcccc
     4981 tccatcgaca agggcgaact ggcgctgggt cagaacatgc gcgtcgcctt catgccgtgg
     5041 aatggctaca acttcgagga ttcgattctg atctccgaac gggtggtgca ggaggaccgt
     5101 ttcaccacca tccacattca ggagctgacc tgtgtggcgc gcgacaccaa gctgggaccc
     5161 gaggagatca ccgccgacat tcccaatgtc ggtgagtccg cgcttggcaa gctcgatgag
     5221 gccggcgtgg tcttcatcgg cgccgaggtc aatgccggcg acattctggt cggcaaggtc
     5281 acaccgaagg gcgaggcaca gctgaccccg gaagagaaac tgttgcgggc gatctttggg
     5341 gaaaaggctt cggatgtgaa ggacacctcg ctgcgggtgc cttccagcac caagggcacg
     5401 gtgatcgacg tgcagatctt cacccgcgac ggcgtcgaaa aagacagccg ggcgctgcag
     5461 atcgagcaga tgcagttgaa cgagattcgc aaggatctga aggaagagca gcgcatcatc
     5521 cgcgacgcca gctacgaacg gctgcgcgcg gcactggtcg gacagccggt ggtgcgtgcg
     5581 cctggtctca agaagggtga tccgatccag ccggactatc tggatcagct ggcggcggcc
     5641 gactggttcc gtatccgcat gtccgacgag ctgctcaatg aacagctgga gcagaccgaa
     5701 gcccggctga aggagcatca gcaggagctg gatcgcaaat tcgaggacaa aaggcgcaag
     5761 ctgaccagcg gggccgatct ggcgccgggg gtgttgaaga tcgtcaaggt ctatctggcg
     5821 atcaagcggc aggcgcagcc cggcgacaag atggccgggc ggcacggcaa caagggggtc
     5881 atctcggtca tcatgccggt cgaggacatg ccctatgacc agaatggcga gccgatcgac
     5941 atcgtactca acccgctggg tgttccttcg cggatgaacg tcgggcaggt gttggagact
     6001 cacctggggt gggcagccaa ggggctgggg cgcaagatcg gcgagctgct cgagcgcaag
     6061 gccagcatcg acgaactgcg cagctttctc gaccagatct acaaccgtgt cggcgggcgc
     6121 aaggagcggc tggatgagct gaccgaggcc gagctgatcg agctgtccga aaacctcact
     6181 gcaggggtgc cgatggccac gccggtcttc gatggcgcca gcgaggacga gatcaagtcg
     6241 atgctgcggc tggccggcct gccggagagt ggccagaccc ttctgtatga tggacgcacc
     6301 ggtgaagcct tcgaccgtcc ggtgacggtt ggctacatgt acatgttgaa gctgaaccac
     6361 ctggtcgatg acaagatgca tgcgcgctca accggctcct acagcctggt gacccagcag
     6421 ccgctgggtg gcaaggcgca gttcggcggg cagcggttcg gcgaaatgga ggtctgggcg
     6481 ttgaaggcct atggtgccgc ctacacgctg caggagatgc tgacggtcaa atcggacgac
     6541 gtgaatggcc gtacccgcat ctacaagaac atcgtcgatg gcgactatcg catggatccg
     6601 ggcatgcccg aatcgttcaa tgtgctggtc aaggagattc gctccctggg catcaacatc
     6661 gaactggaga accagtgagt ctgcgcggcc ccgttcgatt tcaggtcata gatagtcaag
     6721 tgtcgctccg gatggaggta ttgctttgaa agatttactc agcctgctga agacccagac
     6781 ccaaaccgaa gagttcgact tcatccgcat cggactcgcc tcgccggaga tgatccgctc
     6841 ctggtcgttt ggagaggtca agaagcccga gaccatcaac taccggactt tcaagccgga
     6901 gcgtgatggg ctgttctgcg ccaagatttt cggtccgatc aaggattacg aatgtctctg
     6961 cggcaagtac aagcgcctta agcaccgtgg tgtgatctgc gagaagtgtg gcgtcgaggt
     7021 ggcgctgacc aaggtgcgcc gcgagcggat gggccacatc gagctggcca gtccggtggc
     7081 gcacatctgg tttctgaagt cgctgccatc gcgcatcggt ttgctgctgg acatgacgct
     7141 gcgcgacatc gagcgggtgc tctactttga atcctttgtt gtgctcgatc cggggatgac
     7201 caccctggag aaggggcaga tgctcagcga cgagcagtat tacgaggcgc tcgaggagtt
     7261 cggcgacgaa ttcaccgcca agatgggcgc ggaggcgatt caggagctgc tcaaggagat
     7321 cgacctcgaa gctgaaatca cccgcctgcg tgcggagatt ccagaaacca gttccgagac
     7381 caagctgaag aagtactcca agcggttgaa gctgctcgaa gggctgctcg gctctggcga
     7441 tcggccggag tggatggtgc tgaaggtgct gccggtgctg ccgcccgacc tgcgtccact
     7501 ggtgccgctg gatggtggcc gcttcgccac ctccgacctg aacgacctct atcgccgcgt
     7561 catcaaccgc aacaaccgcc tcaagcggct gctcgatctc aatgcgccgg acatcatcgt
     7621 ccgcaacgaa aagcgcatgc tgcaggaggc ggtcgatgcg ctgctcgaca acggccgccg
     7681 tggccgcgcg atcaccggat cgaccaagcg tccgctgaag tcgctggccg acatgatcaa
     7741 gggcaagcag ggtcggttcc ggcagaacct gctgggcaag cgggtcgact actcgggccg
     7801 ttcggtgatc gtggtcggcc cgacattgcg gctgcatcag tgcggattgc cgaagaaaat
     7861 ggcgatcgag ctgttcaagc ccttcatctt cggcaagctg gagcacagcg gactggccac
     7921 caccatcaag gccgccaaga agatggtgga gcgggagacc cccgaggtgt gggacatcct
     7981 ggccgaggtg atccgcgagc acccggtgct gctcaaccgg gcgccgacac tgcaccgtct
     8041 cggcattcag gcgttcgaac cggtgctgat cgaaggcaag gcgatccagt tgcatccgct
     8101 ggtctgtgcc gcctacaacg ccgacttcga cggcgaccag atggccgtac acattccgct
     8161 gacgctcgaa gcacagctcg aggcgcgcag cctgatgatg tcgaccaaca acattctctc
     8221 acccgccaac ggcgagccga tcatcgttcc gtcacaggat gtggtgctgg gcctgtacta
     8281 catgacccgc gagcgcatca atgcgcgtgg tgaaggcatg tgtttcagcg atgtcgagga
     8341 ggtcgaccgc gcctaccact gcggtcaggt cgatctgcat gccaaaatca aggtgcgcct
     8401 cgatgagatg ctgatcaacg cagagggcgc cgccgcccgc agcgcattga tcgacaccac
     8461 ggtcggtcgc acgctgttgt cgaagattct gcccaatggg gttcccttcg acttgatcaa
     8521 ccggccaatg accaagaagg ccatctcccg catcatcaac gcctgctatc gcaatgtcgg
     8581 gctgaaggag acggtgatct tcgccgacca gttgatgtac accggcttct tctattcgac
     8641 caagtcaggc gtctcgatcg gcatcgacga tctggtcatc ccgcaggcca aggcgcggtt
     8701 catcgaccag gccgaagaag aggtcaagga gatcgaggcg caatatgcct cgggattggt
     8761 gacccagggc gagaagtaca acaaggtgat cgacatctgg tcgcggacca acgacctggt
     8821 ggccaaatcg atgatggcaa ccttgggcag tgagcaggtg cgcgaccgcg acggcaacga
     8881 ggtgcagcag gagtcgttca actcgatcta catgatggcc gactccggcg cgcggggctc
     8941 ggctgcacag atccggcagc tggccgggat gcgcgggctg atggccaaac cggatggctc
     9001 catcatcgaa acgccgatca ccgccaactt ccgcgagggg ctatcggttc tgcaatattt
     9061 catctcgacc cacggcgcgc gcaaagggtt ggccgacacg gcgctgaaga ccgccaactc
     9121 gggctacctc acccggcgac tggtggatgt ggcgcaggat ctggtggtca ccgaagtcga
     9181 ctgcggcacc cgcaacggtg tgctgatggc accggtgatc gaaggcggcg atgtgatcga
     9241 ggcgctcggc gagcgcatcc tcggccgcgt gctggccgag gatgtgatca atcgcaccac
     9301 cgaagaggtg gcgattccgg cgggtacatt gatcgacgaa cagtggattc agcgcatcga
     9361 ggagctgggc atcgacgagg tgcaggtccg ttcggccatc acctgcgaga cccgccatgg
     9421 catctgctcg agctgctatg gacgtgacct ggcccgcggc catgtggtca acgtcggtga
     9481 ggcgatcggt gtcgtcgcgg cgcagtcgat cggcgagccg ggcacccagt tgacgatgcg
     9541 taccttccac atcggtggtg cggcgtctcg ggcatcggcc accgacagca tccaggtgaa
     9601 gcatggcggc aagattcgtc tgcacaacct caaattcgtc gagcagcaca atggcaacct
     9661 ggtggcggtc tcccgctccg gtgaactggc gatcgccgat caggatggcc gtgagcgcga
     9721 gcgttacaag gtgccctacg gtgcggtgct gacggtgcgc gatgagcagg tggtcgaggc
     9781 gggaacggtc gtggccaaat gggatccaca cacccatccg atcatcaccg aggtggccgg
     9841 aaccgtccgc ttcgtcggca tggaggaggg tatctccatc cgccaccaga ccgacgaggt
     9901 gaccggactc tccagcatct cggtgatgga cccttcggag cgtccggcgg caggcaagga
     9961 tctgcgtccg gccatcatgc tgctcgatga gcagggcaac gaaatcctgc tggcgggcag
    10021 caacctgccg gcgcactacc tgctgcctgc caaggcgatc gtcagtctgg cggccaatgc
    10081 ccgcgtcgac gtcggcgatg tgctggcgcg catcccgcag gaaggtctga agacgcggga
    10141 catcaccggc ggtctgccgc gcgtggccga cctgttcgag gcgagaaaac ccaaggagtc
    10201 ggcgattctg gcggagatct ccggggtggt cagttttggc agggagacca agggcaagcg
    10261 gcggctgatg atcacgccgg ccgatggcgt caatccgatc gatggcagtt cgcactacga
    10321 ggagctgatt ccgaagtggc gcacactgtc ggtcttcgag ggcgaattcg tcgagaaggg
    10381 cgaggtggtc tccgaggggg cgaccaatcc gcacgacatt ctgcgtctga agggcgtcaa
    10441 cgagctggcc aagtacatcg tcagtgaaat ccaggatgtc taccggctgc aaggggtcaa
    10501 gatcaatgac aagcacatcg aagtgatcat ccggcagatg ttgcgcaagg tggacatcgt
    10561 cgatgccggt gactccagct tcatcaaggg tgaacagtcc gagtatgcgc tggtgctcga
    10621 ggagaatgac cgtctggttg ccgagggcaa aaccccggcg ctgttcgagc gggttctgct
    10681 gggcatcacc aaggcttctc tggcgaccga atccttcatc tcggcggcct ccttccagga
    10741 gaccacccgc gtgctgaccg aagctgccgt gaccggcaag caggatcaac tgcgcggtct
    10801 gaaggaaaat gtcgtcgtcg gccggttgat cccggctgga accgggctct cctaccacag
    10861 cgaagcgcgg cgcaggcgcc gccgcggcga gatggagagc acctcgacgg ccagtgaggt
    10921 cgaacaggcg ctgagtgaag agctgaagtc gatctgagag ggtgctggac ccatccggtc
    10981 gcctgcgcgg ccggatgggt cggcttgcag cagcaggaaa agttgacgtg aagtggcgtg
    11041 atcattagaa tccgccggcc tgttggcagg ggccttttcg agtgttcctg ccttttttat
    11101 ttatgaagaa atcttgatat ggagtttggt tgtatggcga cgatcaacca attggttcgc
    11161 aagccgcgca agcgcagggt cgtgaagagc gatgtccccg cgctgcaatc ctgtccgcag
    11221 cgtcgtgggg tctgcacccg cgtctacacg acgaccccga aaaaacccaa ttcggcgctg
    11281 cgcaaggtgt gccgggtgcg gttgaccaac ggtttcgagg tctcctccta cattggcggc
    11341 gaaggccaca atctgcagga gcactcggtg gtgctgattc gtggcgggcg ggtcaaggat
    11401 ctgccagggg tgcgctacca caccgtccgc ggcagcctcg acacctccgg ggtcaaggac
    11461 cggcggcagg cgcgttccaa gtatggaacc aagaagccaa agtcgtaaaa gacgttggca
    11521 gtaaggccgg agagctgctt gtgcagctgt tccggatcaa cctgaagagc atcaaaagag
    11581 gtaactgttg tgccaagaag acgcgtcatt gccaagcgtg agatcctgcc cgatgccaaa
    11641 tatggcagcc cgctggtcgc cagattcatg aatcacgtga tggaagatgg caagaaatcc
    11701 atcgccgaaa gaatcgtcta cggtgcgctc gatatcgtcg ccgagcgtac caagggcgat
    11761 ccgctcaaga ttttcgagag tgcgctcgat gcagtggcgc cactggtcga ggtgaaatcg
    11821 cgacgggtgg gtggcgcaac ctaccaggtt ccggtcgaag tcagggcttc gcgccgtggt
    11881 gcgttgtcga tgcgctggct ggtgcaatac tcccgtgccc gcagtgaaaa atcgatgccg
    11941 caacggctgg cgggcgaaat catggatgcc gcgcagggtc gcggcgcagc ggtcaagaag
    12001 cgtgaagatg tccaccggat ggccgaagcc aacaaggcgt tctcgcacta ccgtttttaa
    12061 tctcgcagta atctctgatc caagttcagg agcagtttcg tggcacgtac gaccccgttg
    12121 aaacgatacc gaaatatcgg catttgcgcg catgtggatg caggcaagac caccaccacc
    12181 gagcgggtgc tgctctatac cggcgtctcg cacaaactgg gtgaggtcca cgaagggacc
    12241 gccacgatgg actggatggc gcaggagcag gagcgtggca tcaccatcac ctccgcggcc
    12301 accacctgtt tctggaaagg gatggaccag cagttcgacc agcaccgcat caacatcatc
    12361 gacaccccgg gccacgtcga tttcacgatc gaggtggaac gttcgctgcg ggtgctggac
    12421 ggtgccgtgg tggtcttctg cgcaaccgca ggggtggagc cgcaatcgga aacggtctgg
    12481 cgtcaggcca acaaatatgg cgtgccgcgc atcgtctttg tgaacaagat ggaccgggcg
    12541 ggggccgatt tcctgcgtgt cgtcgatcag atcaagaacc gtctgggtgc caatccggtg
    12601 ccgattcagt tgccgatcgg cagtgaggag aatttcgagg gcgtggtcga cctgttgcgc
    12661 atgaaggcga tctactggaa cgaagccgac gcgggcatga cctacgaggc gcggccgatc
    12721 tctgccgaaa tgaaggagat ctgcgacagc tatcgcgagc tgctgatcga gagtgccgcc
    12781 gaggccagcg atgaactgat ggagaagtac ctcgaaggca gtgagctcac caccgacgag
    12841 attcgtcagg cgctgcgcgc gcgcaccatc cgcggcgaaa tcgtgccggc gctctgtggt
    12901 tccgccttca agaacaaggg cgtgcaggcc atgctggatg cggtcatcga atatctgcca
    12961 gcgcccgacg aggtcaaggc catcagcggt atgctggagg atggcgagac ggtgatcgag
    13021 cgcaaatctt ccgacgacga gccctttgcg gcgctggcct tcaagatcgc caccgacccc
    13081 tatgtcggca ccctgacctt tttccgagtc tactccggcg tgctgagctc gggcgatgcg
    13141 atctacaact cggtcaaggg caagcgcgag cgtgtaggcc gcatggtgca gatgcatgcc
    13201 aatgaccgca ccgaaatcaa agaggttcgc gcgggtgaca tcgctgctgc ggtgggtctg
    13261 aaagatgtca ccaccggtga cacgctgtgc gacctcaaca agccggtggt gctcgagcgg
    13321 atggagttcc cggagccggt catctcggtt gcggtggagc cgaaatcgaa gcccgatcaa
    13381 gagaagatgg gggtggcgct cggcaagctg gcgcaagagg atccgtcatt ccgggtcaag
    13441 accgacgaag agtcgggtca gaccatcatc tccggcatgg gcgagctgca cctcgacatc
    13501 atcgtcgacc ggatgcggcg tgaatttggc gtcgaggcca acatcggcaa gccgcaggtg
    13561 gcctaccgcg aaaccattcg caagaaggtc gaggttgaag gcaagttcgt ccggcaatcg
    13621 ggcggtcgtg gccagtatgg tcatgtctgg ctgcggctcg aaccgctccc cgagggtgaa
    13681 gagtatcaat tcgtcaacga agtggtgggc ggcaccgtgc cccgcgaata tattccagcg
    13741 gttgacaagg gggtgcgcga gcagatgcag aatggcatct tggccggcta cccgctgctg
    13801 gcactgaagg ccacgatttt cgatggctca taccacgatg tcgactcaaa tgagatggcc
    13861 ttcaaggtcg caggatcgat ggcgctgaag aagggtgcgc tcgaagcgga cccggtactg
    13921 ctcgaaccga tcatgaaggt tgaagtggtc acgccagagg attacatggg agatgtggtg
    13981 ggcgacctga accggcgccg cggcatgatc cagggcatgg atgattcccc ttctggcaag
    14041 gtggtcaccg ccgaggtgcc actggccgag atgttcggct actccaccga cctgcgttcc
    14101 gcaacgcagg ggcgagcgac ctattcgatg gagttcaagc gctacgccga gactcccaac
    14161 aacattgctg aagccatcat cagcaagaac aagtaaaaca actgattcat tcccttgatg
    14221 caaggagggg tgtgccatgg cgaaagagaa gtttgagcgt aagaagccgc acgtcaacgt
    14281 ggggac
//
LOCUS       DAORCQ010000062        14110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2788_length_14110_cov_5.737531, whole genome shotgun sequence.
ACCESSION   DAORCQ010000062 DAORCQ010000000
VERSION     DAORCQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_14110_cov_5.737531"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKI23_07690"
     CDS             <1..787
                     /locus_tag="PKI23_07690"
                     /inference="COORDINATES: protein motif:HMM:NF017516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF829 domain-containing protein"
                     /protein_id="HNN36857.1"
                     /translation="SENMDRSTAEHAASIPQPQRQALIFGGFDMQPKHCRGIADLYRQ
                     HGAQSVTPLCHSLQQMTVVRLGDRQARQLAHRFASLTAHDEVVVHLYSGAVFIFFRLL
                     GYLPVQARQAIRAVVFECSPMDCQAEQFGRFASWRLGRDYRRRHAAPFVLLRPLAGIT
                     RRFERDHHAAMRLLDAETAVHFILCDNDPIIDPGYVTAYHRELSDRGHATSLTLHRGA
                     RHCRALSDCPQPYQTDLSHFLDRHWRSPQPVQYPMQRRAVAAL"
     gene            857..1321
                     /locus_tag="PKI23_07695"
     CDS             857..1321
                     /locus_tag="PKI23_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36858.1"
                     /translation="MFNFISWIVDHRRTVVLLVGLITVGLLLPLRHMEVIVDTDQLLP
                     QSHPYVIATKKVEQIFGNRYTVVVGMTARSGTVIEPKLLEKLKGLTEALNRIPGAVRP
                     NQRSLASSKTKNIVGTEEGMSARAMLAKLPQARRNSPHCAQQSTTIQSMTVC"
     gene            1288..4002
                     /locus_tag="PKI23_07700"
     CDS             1288..4002
                     /locus_tag="PKI23_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNN36859.1"
                     /translation="MDNNPVYDGVLISKDRKTAAVVTEVKSNPLGFGAIERDVRAAIA
                     PFADESVELMVTGQPIFLSALEHFSDRMGFLLPIALLVIGLLHFEAFRTIQGLVLPLV
                     TALLAVVWSLGIMTWFGMQLDPFSNVTPILIIAVAAGHAVQILKRYYEAYHAIEPHSE
                     DKRAANRAAVVTAVSAIGPVMVAACVIAAASFLSLLVFGIQSIRTFASFSALGILSAL
                     AIELTFIPAVRAMLPPPSPKLVELEHKRTVWDRIIDRLATVALSPRRGWMIVGGLLLA
                     VVAAGGTTLVVQDNSLRSFFTASQTIRQEDAVMNARLAGSNTLYVLIEGQQDDAIKDP
                     AILNAMLTTQRQLEKDPLVGGTISLADFIVRMNRAMHGDDPATATIPADANLVAQYLL
                     LYSMSGDEGDFNNYVDPSYRNAVIQAFVKTDSSAHVKQLEAQLLPVIRQAFPPDVTVR
                     IAGSITTPTAMSEMIVQGKIMNLLQMAGAILLIASVMFRSLFAGLLLIIPLAMTALVN
                     FGLMGFFGIPLQIATSTVAALAVGIGADYAIYFTYRLREELRRDGDIEAATRRAYATA
                     GKAVLYVATAVAGGYGVLIFSIGFNIHLWLGIMVGVAMLVSALTALTLYPVLLLMLKP
                     GFVFGQARLEPAAATLGLLALLTLWPTDQAHAQQDADALDATTVMQRSYEVTLYKSTR
                     QAATFVLAGSDGKNFERKATGYSKLADDGIHSSRLTRFLSPANISGTAVLSVENSDRE
                     DDVWVYLPALHKVRRLAAGEKRDSFVGTDFSHGDVIGHKVADWNHTLIKNETVDGAQT
                     AVIDSVPRDDTVKSSTGYGRRVSWVRLDNWVLVRSEFYDETGALLKRFSARDLKLVDA
                     AAQRWQPMELYMKNVQTEHESTIRYHEFEVGVAVPANAFSSRALDVQP"
     gene            3999..5282
                     /locus_tag="PKI23_07705"
     CDS             3999..5282
                     /locus_tag="PKI23_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36860.1"
                     /translation="MRQVVSTTGLTLLLLLGAAAQAMEPLPGWQTASEAIGLSQSLRF
                     GYWRSTRSVDDQINLLPASLWLRERYSFDSGMTLHAEGWLQSKDVAHPWQAESRGELR
                     EAYLRDTLGDFDIRFGRQIVAWGRADRINPTDNLSARDLTRMFVEDDDLKRGSTLLRL
                     AHPLGETAELQLYWIPEFRPEVHPLAERIGPFSIAGDDRRPTDNSQHAIKIDGSRDGL
                     DWSLSWFNGHNPSGDLRALDAARPLILVREYARQQTFGADAATVVGNFNLRAEAAYTD
                     FPDRDAGDLSKRPFLFAVFGGDRNLGEQFNLNLQYLLRRVSDFEPLERLANPIERGIA
                     TINAVEAGQRDRYQRGATLRLAWSSSDQRWRAELFALQDFGQHDGIVRTLIRHDLSDD
                     LRLSLGHEWNHGESDTLFGSLHDNNGLFMELRLGY"
     gene            5430..7850
                     /gene="lon"
                     /locus_tag="PKI23_07710"
     CDS             5430..7850
                     /gene="lon"
                     /locus_tag="PKI23_07710"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNN36861.1"
                     /translation="MSQSIETADEITSSRADEEQAASVPALPGQHLPDKLVLIPVSGR
                     PFFPAQVQPVIVDAEPWHQSFAQVAETDHHLLGLIYVPDFDADSTPVEAFPLIATVVR
                     PHHPHKIDNNIQFIAEGLQRFRILHWISRIPPYLVQVQYLRGSETHSDETQAYAIAII
                     EAIKELLPLSPLYGEELKQYLKRFSPNQPSPLTDFAATLTTATSAELQQVLETENLLQ
                     RMEKVLLLLRKEVQVARLQHQISEEVGQKVGEQQRQFFLREQLKVIQRELGLSKDDRT
                     ADSDEFKRRLQALVVPPHAEKKINDELHKLSILDNGSPEYGVTRNYLDWATSVPWGRF
                     TQDQLDLKQARRVLDSHHDGLDDVKKRIIEFLAIGAYKGEIAGSILLLVGPPGVGKTS
                     IGQSIASALGRKFYRFSLGGMRDEAEIKGHRRTYIGAMPGKLVQALKEVEVSNPVILL
                     DEIDKIGASYQGDPASALLEVLDPEQNSQFLDHYLDLRLDLSKVLFICTANQLDTIPP
                     ALLDRMETIRLAGYITAEKLSIARHHLWPKQLAKAGLKAGQCRISDAALRRVIEDYAR
                     EAGVRHLEQQLARIIRKVVVELLESPGQRFNIGVKDVERHLGGALFRPDQPIRQVGVV
                     TGLAWTALGGATLPIEACRVHAKGRGFKLTGKLGEVMQESANIAYSHIDAQAVKFGID
                     PEFFSSSHIHLHVPEGATPKDGPSAGITLATALVSLASGRNIDRPLAMTGELTLTGQV
                     LPVGGIREKVVAAKRARIAELILPEANRRDFDELPDYLRDGMKVHFVRQFSEIPPIVF
                     APAGRSRS"
     gene            7952..9289
                     /gene="rimO"
                     /locus_tag="PKI23_07715"
     CDS             7952..9289
                     /gene="rimO"
                     /locus_tag="PKI23_07715"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNN36862.1"
                     /translation="MSSSKVGFVSLGCPKALVDSERILTQLRLDGYQIVSHYSEAELV
                     VVNTCGFIDAAKQESLEAIGEAISANGRVIVTGCLGKEAEQIRAQHPQVLAVTGPHAY
                     EAVLSAVHRHLPSRTPYDPFTQLVPPQGIKLTPRHYAYLKISEGCNHRCSFCIIPALR
                     GDLVSRPIGSVMDEAEQLVRAGVRELLVVSQDTSAYGVDLKYRTGFWQGRPLRTRLLD
                     LCQALGELGVWVRLHYVYPYPHVDEIIPLMAEGRILPYLDIPLQHGVPRILKLMKRPA
                     ASEKTLDRIAHWRQICPELTLRSTFIVGFPGETEEEFEALLDFLDAAQLDRVGCFTYS
                     PVEGAAANQLPDAVPEEVKQARYHRFMTHQAKISAERLRRKVGSIQQVLVDEVTPQGA
                     IGRSKADAPQIDGLVYLHGKRQLTVGEWVEARIEHSDEHDLFGLTLPPAEPGH"
     gene            9357..9869
                     /locus_tag="PKI23_07720"
     CDS             9357..9869
                     /locus_tag="PKI23_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNN36863.1"
                     /translation="MEPIRSVCSTTAILMSLLLWPLLATAELLPSADLVLVNKSQRKL
                     YLMREGVIYREYRVALGKNPKGHKQREGDDRTPEGFYWLDWRNPDSQYYKAIHVSYPN
                     EKDRTAAKNRKVKPGGMIMIHGMPNFPSKPAKNYQNRDWTEGCIAVSNEAMEEIWHAV
                     EDDTPILIEP"
     gene            9884..10657
                     /locus_tag="PKI23_07725"
     CDS             9884..10657
                     /locus_tag="PKI23_07725"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNN36864.1"
                     /translation="MPHSNPSPLSRALLWMLGTLLLLPRAGWSSCEAARTALDTSIRA
                     TYSTQLYGLPISSEHRMAPGADGYEVVNKGRVLGFTVLEQSRFRLSAENRLQPLEYRY
                     RRGSEGDKQNLDLRFDWKKHIVVSQRPNDDWQLPLEPLTQDALSQQEQLRLDLICQGS
                     RFDSVTYPVVRNNKMRHYTYVKLEEALLDTAVGPLKTVVIRKVDEKAERDTKVWMAID
                     WDYLLVKLQYREKNGELYRLELDSAELGDRPVTAFQPAP"
     gene            10816..11058
                     /locus_tag="PKI23_07730"
     CDS             10816..11058
                     /locus_tag="PKI23_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNN36865.1"
                     /translation="MQLLIKKWGNSASVRIPAAIMEAAHLHIDDAVDVREEDGRIVIE
                     PIRPKRYALTELLAGITPHNLHGEADFGPAIGRESG"
     gene            11058..11396
                     /gene="mazF"
                     /locus_tag="PKI23_07735"
     CDS             11058..11396
                     /gene="mazF"
                     /locus_tag="PKI23_07735"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HNN36866.1"
                     /translation="MAAPYVPDAGDIVWLQFNPQAGHEQAGHRPALVLSPVAYNHKSG
                     LMLCCPLTTQIKGYPFEVMIAGQVASAALADQVKSLDWSARKAVPKGKATAAELAEVR
                     AKLLALLAPV"
     gene            11529..12662
                     /locus_tag="PKI23_07740"
     CDS             11529..12662
                     /locus_tag="PKI23_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase/peptidase C39
                     family protein"
                     /protein_id="HNN36867.1"
                     /translation="MIRPARLSDLTALLAIENRCFQTDRLSRRNFRYLLTQAKAATLL
                     LEADGAVQGYVMLLFNQGTSLARLYSVAVDPALRGQGAGRQLIEAAEACALAHECLTL
                     RLEVRADNPPAIALYRRLGYHQFGIYEDYYDDHARALRFEKRLAPELNVELAAVPYYR
                     QTLDFTCGPSALMMAMKTLQPELPLDRRLELNLWRESTTIFMTSGHGGCSPYGLALAA
                     HHRGFDVEIHVKDDAALFVDSVRDPEKKAVIRLVQEAFIAELSQLPIPIHYGVVTIEE
                     LEAARRRGGVPVVLISSYRIYGEKFPHWVVVTGFDERCIYVHDPYLDENSDRVIMDCI
                     NMPILRRDFARMNRYGKAAQMATLVVYPRPHGGAIEGKPSATP"
     gene            12684..>14110
                     /locus_tag="PKI23_07745"
     CDS             12684..>14110
                     /locus_tag="PKI23_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HNN36868.1"
                     /translation="MSRAIILVEELEDWPATFPAVELVRARDYLASSALQETRALRVI
                     NLCRGYHYLTLGYYCSLLGEARRHRLLPSVRTLADLDRKNLYELVTLELNQEMLRPAM
                     ESAAAEELSFNIYFGKSERPGFTWLARALYEAFPAPILRVNLHLGEKSEVRAIEAGAL
                     HELDDAQQRLFLAALEALLNRGWRRPRARRHFRYDMAILHDPAEKLAPSNPRALARMI
                     RAGRRLGINVELVTRRDYDRLAEYDALFIRETTHIDHHTYQFARKAEREGMVVIDDSD
                     SIVRCTNKVYLAELIDAHELAAPTTVIINRDEIDRVEQQLDYPMVLKIPDGSFSRGVH
                     KVQNRTELHQVAAQLFASSDLILAQAFVYTEFDWRIGVLNRKPLFACQYFMSKHHWQI
                     VKHEGEGRFKVGNHRAVPVAEVPEAVIQLALKAANLIGNGLYGVDIKQSGNRLYLIEI
                     NDNPNLDAGVEDQILGMALYQQLMQ"
BASE COUNT         2620 a         4734 c         4335 g         2421 t
ORIGIN      
        1 ttcagaaaac atggaccgtt caacggcaga gcacgctgca tcgatccccc agccgcagcg
       61 ccaggcactg atcttcggcg gttttgacat gcagccgaag cactgccgtg gcatcgccga
      121 cctctaccga cagcacggcg cacagagcgt gacgccgctg tgccactcat tgcagcagat
      181 gacggtggtc cggctgggag accggcaggc acgccaactg gcccaccgct ttgcctcgtt
      241 gacagcgcac gacgaggtcg tggtgcatct ctactccggc gccgtcttca tcttctttcg
      301 tctgctgggc tacctgccag tgcaggcccg ccaggcgatc cgcgcggtgg tcttcgagtg
      361 ttcgccaatg gactgtcagg ccgaacagtt cggccgcttc gccagttggc gactgggccg
      421 cgactaccgt cgtcgccatg cggcgccctt cgtgctgcta cggccgctgg cgggcatcac
      481 ccgccgtttc gaaagagatc accacgccgc gatgcgactg ctcgatgccg aaacagcggt
      541 ccatttcatc ctctgcgaca atgacccgat catcgacccc ggctatgtca cggcctacca
      601 ccgggagctc tcggacagag gccacgccac ctcgctgaca ctgcaccgcg gcgcccgcca
      661 ctgccgggcg ctgtcggact gtccgcaacc ctatcaaacc gacctgtcgc actttctcga
      721 ccgccactgg cgcagcccac aacccgtgca gtacccgatg caacggcggg cggttgccgc
      781 actgtgagcg gctgaaaacc actgcccgac gcatgtccaa caccttctta caataaccac
      841 aacaaggaga aaccctgtgt tcaacttcat ctcctggatc gtcgaccacc gccgcaccgt
      901 cgtgcttctg gtcggcctga tcactgtcgg actgctgctg ccgctgcgcc acatggaggt
      961 gatcgtcgac accgaccagt tgctgccgca gagccacccc tatgtgatcg ccaccaaaaa
     1021 ggtcgagcag atcttcggca accgctacac ggtggtggtg ggcatgaccg cccgcagcgg
     1081 cacggtgatc gagccgaagc tgctggaaaa attgaagggg ctcaccgagg cactgaacag
     1141 gatccccggc gcagtgcggc ccaaccagcg cagtctggcc tccagcaaga ccaagaacat
     1201 cgtcggcacc gaagagggga tgagcgcgcg ggcgatgctg gccaagctgc cgcaggcgag
     1261 gcggaactcg ccgcactgcg cacagcaatc gacaacaatc cagtctatga cggtgtgctg
     1321 atctcgaagg accgcaagac cgccgccgtg gtcaccgagg ttaaatccaa cccgctcggc
     1381 ttcggcgcca tcgagcgtga cgtgcgcgcc gccatcgccc cctttgccga tgaaagtgtc
     1441 gaactgatgg tgactggcca gccgatcttc ctgtcggcgc tggagcactt ttcggaccgc
     1501 atgggctttc tgctgccgat cgccctgctg gtgatcgggc tgctgcactt cgaggccttt
     1561 cgcacgatac aggggctggt gctgccactg gtgacggcgc tgctggcagt ggtctggagc
     1621 ctcggtatca tgacctggtt cggcatgcag ctcgacccgt tcagcaatgt cacgccgatt
     1681 ctgatcatcg ccgtggcggc tggccatgcg gtgcagattc tgaagcgcta ctacgaggcc
     1741 tatcacgcca tcgagccgca cagcgaagac aaacgcgccg ccaaccgcgc agcggtggtc
     1801 acggcagtca gcgccatcgg tccggtgatg gtggcggcct gcgtcatcgc tgcggccagc
     1861 tttctgtcat tgctggtgtt cggcatccag tcgatccgca ccttcgccag cttctcggcg
     1921 ctcggtattc tcagcgcgtt ggcgatcgaa ctgaccttca tccccgccgt gcgggcgatg
     1981 ctgccgccgc ccagtccgaa gctggtcgaa ctcgaacaca agcgcacggt gtgggaccgc
     2041 atcatcgatc ggctcgccac ggtcgccctg agtccaagac gcggctggat gatcgtcggc
     2101 ggcctgctgc tggcggtggt tgcggccggc ggcaccacac tggtggtgca ggacaactcg
     2161 ctgcgttcct tcttcactgc gagtcagacc atccggcagg aggatgcggt gatgaatgcc
     2221 cgcctggccg gcagcaacac gctctatgta ctgatcgagg gtcagcagga cgacgccatc
     2281 aaggatccgg ccatcctcaa cgcgatgctg accacccagc ggcaactgga aaaagatccg
     2341 ctggtgggcg gcaccatctc gctggccgac ttcatcgtgc gcatgaaccg ggcgatgcat
     2401 ggcgatgatc cagccaccgc caccatcccg gccgatgcca atctggtcgc ccagtacctg
     2461 ctgctctact cgatgtcggg cgatgagggc gacttcaaca actatgtcga cccgagctac
     2521 cgcaacgcgg tgattcaggc cttcgtcaag accgacagct cggcccatgt caaacagctc
     2581 gaggcgcaac tgctgccggt gatccgccag gcctttccgc ccgatgtcac cgtgcgcatc
     2641 gccggcagca tcaccacccc cactgcgatg agcgagatga tcgtgcaggg caagatcatg
     2701 aacctgctgc agatggccgg ggcgattctg ctgatcgcca gtgtgatgtt ccgttcactg
     2761 tttgccggac tgctgctgat catcccgctg gcgatgaccg cgctggtcaa cttcggcctg
     2821 atgggcttct tcggcattcc gttgcagatc gccacctcga cagtggcggc gctggcggtc
     2881 ggcatcggcg ccgactatgc gatctacttc acctaccgac tgcgcgagga gttgcgccgc
     2941 gacggtgaca tcgaggccgc gacccgccgc gcctacgcca ccgccggcaa ggcggtgctc
     3001 tatgttgcca ctgcggtggc cggcggctat ggcgtgctga tcttctcgat cggcttcaac
     3061 atccacctct ggctcggcat catggtcggc gtcgccatgc tggtcagcgc gctgaccgcc
     3121 ctgacactct acccggtgct gctgctgatg ctgaaaccgg gtttcgtctt cggtcaggcc
     3181 cggctcgaac cggccgcggc gacgctcggc ctgctggcgc tgctgacgct ctggccgacc
     3241 gatcaggccc atgcgcagca ggatgccgac gcgctcgatg ccaccacggt gatgcagcgc
     3301 agctatgaag tcacgcttta caagagcaca cggcaggcgg ccacctttgt gctggccggc
     3361 agcgatggca agaacttcga acgcaaggcg acgggctatt caaaactggc cgatgacggc
     3421 atccacagct cacggctgac ccgttttctg tcaccggcca acatcagcgg cactgccgtg
     3481 ctctcggtcg agaacagcga ccgagaggat gacgtctggg tctatctgcc ggcgctgcac
     3541 aaggtgcgcc gcctggccgc cggcgagaaa cgcgactcct tcgtcggcac cgacttcagc
     3601 catggcgacg tgatcgggca caaggtggcg gactggaacc acacgctgat caagaacgaa
     3661 accgtcgatg gcgcgcagac cgccgtcatc gacagcgtgc cgcgtgacga caccgtcaag
     3721 agctccaccg gctacggtcg gcgtgtctcc tgggtgcgtc tcgacaactg ggtgctggtg
     3781 cgcagcgagt tctacgacga gaccggcgcg ctgctcaaac gcttctcggc gcgtgatctc
     3841 aagctggtcg atgccgccgc ccagcgctgg cagccgatgg agctctacat gaagaatgtc
     3901 cagaccgagc atgagtcgac catccgctat cacgagttcg aggtgggggt ggccgtaccg
     3961 gccaatgcct tctcttcccg tgcgctcgat gtgcagccat gaggcaggtc gtcagtacga
     4021 cgggcctcac cctcctgctc ctgctgggcg ccgctgcgca ggcgatggag ccactgccgg
     4081 ggtggcagac ggccagcgaa gcaatcggtc tcagccagtc gctgcgcttc ggctactggc
     4141 gctcgacccg cagtgtcgat gaccagatca acctgctgcc ggcatcgctg tggctgcgtg
     4201 agcgctacag cttcgactcc gggatgacgc tccatgccga gggctggctg cagagcaagg
     4261 atgtcgccca cccatggcaa gcggagtcac gcggcgaact gcgcgaagcc tacctgcgcg
     4321 acaccctggg tgatttcgac atccggttcg gccgtcagat cgtcgcctgg ggtcgcgccg
     4381 accgcatcaa tccgaccgac aacctgagcg cccgcgatct gacacggatg ttcgtcgagg
     4441 atgacgatct caagcgcggc agcactctgc tgcggttggc ccatcccctg ggtgagacag
     4501 ccgagcttca gctctactgg attccggagt tccgccccga ggtgcatccg ctggccgaac
     4561 ggatcggccc cttctcgatc gccggcgacg accgccgtcc cactgacaac agccagcacg
     4621 ccatcaagat cgatggcagc cgcgatggcc tcgactggtc gctctcctgg ttcaacggtc
     4681 acaaccccag cggcgacctg cgcgcgctcg acgccgcccg gccgctgatc ctggtgcggg
     4741 agtatgcccg ccaacagacc tttggcgccg atgccgcgac cgtcgtcggc aacttcaacc
     4801 tgcgcgccga ggccgcctac accgactttc ccgaccgtga cgcgggcgac ctctccaagc
     4861 gccccttcct gtttgccgtc tttggcggcg accgcaacct gggtgagcag ttcaatctga
     4921 atctgcaata cctgctgcgc cgggtgtcgg attttgaacc gctggagcgt ctggccaacc
     4981 cgatcgaacg cggcatcgcc accatcaatg ccgtcgaggc cggccagcgc gaccgttacc
     5041 agcgcggcgc aacgctgcgg ctggcctgga gcagcagcga ccagcggtgg cgggccgagc
     5101 tgttcgcgct gcaggacttc ggtcagcatg atggcatcgt ccgcaccctc attcgccatg
     5161 acctcagcga tgatctgcgc ctgagcctcg ggcacgaatg gaaccatggc gagagcgaca
     5221 cgctgttcgg cagcctgcac gataacaacg gcctgttcat ggagctgcgg ctcggttatt
     5281 gagccccggc ctcgcgccgc accacgctcc cgtcaccctc gatcgccaag gattgaagtc
     5341 gcgcctgtca cccccatttc cgtctcatga ccgcggtgga cgcaatccag cggtccaccc
     5401 tttccatgag accgcaggcg caagatccca tgagtcaatc gatcgaaacc gcagacgaga
     5461 tcacctcctc ccgagccgat gaggaacagg ccgccagtgt gccggcgctg ccgggacagc
     5521 acctgcccga caagctggtg ctgattccgg tcagcggacg gcccttcttt ccggcccagg
     5581 tgcagccggt catcgtcgat gccgagccct ggcatcagag ctttgcgcag gtggccgaga
     5641 ctgaccacca tctgctcggg ctgatctatg tccccgattt cgatgccgac tcgacgccgg
     5701 tcgaggcctt tccattgatt gccaccgtgg tgcggcccca ccatccgcac aagatcgaca
     5761 acaacatcca gttcatcgcc gagggattgc aacggttccg gattctgcac tggatcagcc
     5821 gtataccgcc ctacctggtg caggtgcagt acctgcgcgg cagcgagacc cacagcgacg
     5881 aaacccaggc ctatgccatc gccatcatcg aggcgatcaa ggagctgctg ccgctgagcc
     5941 cgctctatgg cgaagagctg aagcagtacc tgaagcgctt cagtcccaac cagccctcgc
     6001 cgctgaccga cttcgccgcc acgctgacca ccgccacctc ggccgaactc cagcaggtgc
     6061 tggagaccga aaacctgctg cagcggatgg agaaagtgct gctgctgctg cgcaaggagg
     6121 tgcaggtcgc ccggctgcaa catcagatca gcgaggaggt ggggcagaag gtcggcgagc
     6181 agcagcggca gttctttctg cgcgagcagc tcaaggtgat ccagcgcgag ctggggctga
     6241 gcaaggatga ccgcaccgcc gacagcgatg agttcaagcg ccggctgcaa gcgctggtgg
     6301 tgccgcccca tgccgaaaaa aagatcaacg acgaactgca caagctgtcg attctcgaca
     6361 acggctcacc cgaatatggc gtgacccgca actacctcga ctgggccaca tcggtaccct
     6421 ggggccgctt cacccaggat cagctcgatc tgaagcaggc gcgacgggtg ctcgacagcc
     6481 accatgacgg gctcgacgac gtcaagaaac ggatcatcga gttcctcgcc atcggcgcct
     6541 acaagggcga gatcgcaggc tcgatcctgc tgctggtcgg tccgccgggc gtcggcaaga
     6601 cctcgatcgg ccagtcgatc gccagcgcac tgggccgcaa gttctaccgc ttcagcctgg
     6661 gcggcatgcg cgacgaagcc gagatcaagg gccatcgccg cacctacatc ggtgccatgc
     6721 cgggcaagct ggtgcaggcg ctgaaggagg tcgaggtcag caatccggtg atcctgctcg
     6781 atgagatcga caagatcggt gcctcctacc agggcgatcc cgcctcggcg ctgctcgaag
     6841 tgctcgatcc ggagcagaac agccagtttc tcgaccacta tctcgacctg cggctcgatc
     6901 tctccaaggt gctgttcatc tgcaccgcca accagctcga caccatcccg ccagcgctgc
     6961 tcgaccgcat ggagaccatt cgccttgccg gctacatcac cgccgaaaag ctgtcgattg
     7021 cccgtcacca cctctggccc aagcagctcg ccaaggccgg attgaaagca ggccagtgcc
     7081 ggatcagcga tgccgcgctg cggcgcgtga tcgaggatta cgcccgcgag gccggcgtgc
     7141 gtcacctcga acagcaactg gcgcgcatca tccgcaaggt ggtggtggag ctgctcgaat
     7201 cacccggcca gcgcttcaac atcggcgtca aggatgtcga gcgccatctg ggcggtgcgc
     7261 tgttccgtcc cgaccagccg atccggcagg tgggtgtggt gaccgggctg gcctggaccg
     7321 ccctgggtgg cgccaccctg ccgatcgagg cctgccgggt ccatgccaag gggcgcggct
     7381 tcaagctgac cggcaagctc ggcgaggtga tgcaggagtc ggccaacatc gcctacagcc
     7441 acatcgacgc acaggcggtg aagttcggca tcgatccgga gttcttcagc agcagccaca
     7501 tccacctgca tgtgcccgaa ggcgccaccc ccaaggatgg cccgagtgcc ggcatcaccc
     7561 tggccaccgc gctggtctcg ctcgcctcgg ggcggaacat cgaccggccg ctggcgatga
     7621 ccggtgaact gaccctcacc ggacaggtgc tgccagtcgg cggcatccgc gaaaaggtgg
     7681 tggcagccaa gcgggcccgc atcgccgagc tgatcctgcc cgaggccaac cggcgcgact
     7741 tcgacgaact gccggattac ctgcgtgacg ggatgaaggt ccacttcgtg cgccagttca
     7801 gcgagatacc ccccatcgtc tttgccccgg cagggcgctc cagaagctga gcagtcgcgg
     7861 ccggcggctc gatcacccgc acggcgtgct ggctgccgcc gtgcggtgat caaggtagaa
     7921 tggccggctt tcacgctgcg ggtacccctt gatgtcctcc tccaaagtcg gcttcgtcag
     7981 cctgggctgt cccaaggcgc tggtggattc agaacggatc ctcactcagt tgcggctcga
     8041 tggttatcag atcgtcagcc actacagcga agccgaactg gtggtggtca acacctgcgg
     8101 cttcatcgat gccgccaagc aggagtcgct ggaggcgatc ggcgaggcca tcagcgccaa
     8161 tggccgggtg atcgtcaccg gctgcctggg caaggaggcc gagcagattc gcgcgcagca
     8221 cccgcaggtg ctggccgtca ccggccccca cgcctacgaa gcggtgctga gcgcggtgca
     8281 tcgacatctg ccctcgcgca ccccgtatga tcccttcacc caactggtgc cgccgcaggg
     8341 catcaagctg acgccgcgcc actatgccta tctgaagatt tccgaagggt gcaaccaccg
     8401 ttgcagcttc tgcatcatcc cggcgctgcg gggtgacctg gtcagccggc cgatcggcag
     8461 tgtcatggat gaggccgaac aactggtcag ggccggcgtg cgcgaactgc tggtggtctc
     8521 gcaggacacc agcgcctatg gcgtcgatct gaaataccgc accggcttct ggcaggggcg
     8581 ccccctgcgc acccggctgc tcgacctctg ccaggcactg ggcgagctgg gcgtctgggt
     8641 gcggctgcac tatgtctatc cttaccctca tgtcgacgag atcatcccgc tgatggccga
     8701 agggcggatc ctgccctacc tcgacattcc attgcagcat ggcgtgccac gcattctcaa
     8761 gctgatgaaa cggccggccg ccagcgagaa gaccctcgat cgcatcgccc actggcggca
     8821 aatctgcccg gagctgacgc tgcgcagcac cttcatcgtc ggctttccgg gcgagaccga
     8881 agaggagttc gaggcgctgc tcgactttct cgacgcagca cagctcgatc gggtcggctg
     8941 cttcacctac tcaccggtcg aaggcgctgc ggccaaccag ttgcccgatg cggtgcccga
     9001 agaggtgaag caggcgcgtt accaccgttt catgacccac caggccaaaa tcagcgccga
     9061 acgcctgcgg cgcaaggtcg gcagcatcca gcaggtgctg gtcgacgagg tgacaccgca
     9121 aggggccatc ggtcgcagca aggccgatgc accgcaaatc gatggcctgg tctatctgca
     9181 tggcaaacgg caactcacgg tcggtgaatg ggtggaggcc aggatcgaac acagtgatga
     9241 acatgacctg ttcggcctga cactgccgcc agcggaaccg ggccattgat tttttgacat
     9301 catgcgttta aatatgaagc taacctgcta taaaccatcc attttatgga gaagaaatgg
     9361 agccgatccg ctctgtctgc agcaccaccg ccatcctgat gtcactgctg ctgtggccgc
     9421 tgctggcgac ggccgagctg ctgccgagcg ctgatctggt gctggtcaac aagtcgcagc
     9481 gcaagctcta tctgatgcgc gaaggggtga tctaccgcga ataccgcgtc gcgctgggca
     9541 agaaccccaa gggacacaag cagcgcgaag gcgatgaccg cacgccggag ggcttctact
     9601 ggctcgactg gcgcaacccc gacagccagt actacaaggc gattcacgtc tcctacccga
     9661 acgagaagga ccgcacggcc gccaagaacc gcaaggtcaa gccgggcggc atgatcatga
     9721 tccacggcat gcccaacttc ccctccaaac cggccaaaaa ctatcagaac cgcgactgga
     9781 ccgagggctg catcgccgtc tccaacgagg cgatggagga gatctggcat gcggtcgagg
     9841 atgacacgcc gattctgatc gaaccctgaa ccgatcacgc aagatgcctc actccaaccc
     9901 ttcaccactg tcgcgcgccc tgctgtggat gctgggcacg ctcctgctgc tgccccgcgc
     9961 cggctggtcg agctgcgagg cggcacggac cgccctcgac acgtcgatcc gcgccaccta
    10021 cagcacccag ctctatggcc tgccgatcag cagcgaacac cgcatggcgc ccggcgctga
    10081 tggctacgag gtggtcaaca aggggcgggt gctcggcttt accgtgctgg agcagagtcg
    10141 ctttcgcctc agtgccgaaa accggctcca accgcttgaa taccgctatc gccgcggcag
    10201 cgagggcgac aagcagaacc tcgacctgcg ctttgactgg aagaagcaca tcgtggtcag
    10261 tcagcgcccc aacgacgact ggcagctccc gctcgaaccg ctgacccagg atgccctgag
    10321 ccagcaggag cagttgcggc tcgacctgat ctgccagggc tcacgcttcg actcggtcac
    10381 ctacccggtg gtgcgcaaca acaagatgcg ccattacacc tacgtcaagc tcgaagaggc
    10441 gctgctcgac accgccgtgg ggccgctcaa gaccgtggtg atccgcaagg tcgatgagaa
    10501 ggccgaacgc gacaccaagg tgtggatggc catcgactgg gactacctgc tggtgaagct
    10561 gcaataccgt gagaagaacg gcgaactcta tcgcctggaa ctcgacagcg ccgaactggg
    10621 cgatcgtccg gtgacggcct ttcagccggc gccttgatgg ccggcactgc cggtctggcc
    10681 tctgcggctg cgcggcctgc ttggccgaca tcgaaaggcc tcggccccac gctccatacc
    10741 catttcgata ctgttgcagc ccggcggccg cttgggctag agtatctaca ttgacgatac
    10801 aaagaagagt ggaccatgca actgctgatc aagaaatggg gcaacagcgc gtcggtgcgc
    10861 atcccagctg cgatcatgga ggctgcccat ctgcacatcg acgatgccgt cgatgtgcgc
    10921 gaggaggatg gccgcatcgt catcgaaccg atccggccaa aacggtacgc attgaccgag
    10981 ctgctcgccg gcatcacgcc gcacaacctg catggtgaag ccgacttcgg gccagccatc
    11041 ggccgggaaa gcggctgatg gcggcaccct acgtgcccga cgccggcgac atcgtctggc
    11101 tgcaattcaa cccgcaggcc ggacatgagc aggccggtca ccgccccgca ctggtgctca
    11161 gtccagtggc ttacaaccac aagagcggac tgatgctctg ctgtccgctg accacgcaga
    11221 tcaagggtta tcccttcgag gtgatgatcg ccggtcaggt ggcgagcgcg gcactggctg
    11281 atcaggtcaa aagcctggat tggagcgcgc gcaaggcggt gccaaaaggc aaggccacgg
    11341 ccgccgaact ggccgaagtg cgtgccaaat tgctggcatt gcttgcaccg gtctgagccg
    11401 tcggccgggt ttgccggcaa tcgggtggcc cacgcggtga aaaaccgcca ccagttccca
    11461 tcagcctgac cgaggctgca cgatctgccc tatcatgacg ggcctgcgac tccaccggca
    11521 gcctgcccat gatccgcccc gcccgcctca gcgacctgac cgccctgctg gcgatcgaga
    11581 accgctgttt tcagaccgac cggctgtcgc ggcgcaactt ccgctatctg ctgacccagg
    11641 ccaaggccgc cacgctgctg ctggaggcag acggcgcggt gcagggttac gtgatgctgc
    11701 tgttcaatca gggcacctcg ctggcccgcc tctactcggt ggcggtcgat cccgctctgc
    11761 gcggccaggg cgctggccgg caactgatcg aggcggccga agcgtgcgcg ctggcgcatg
    11821 agtgcctcac gctgcggctc gaggtgcggg ccgacaaccc gccggccatt gcactctatc
    11881 ggcggcttgg ctaccaccag ttcggcatct acgaggatta ttacgacgac catgcgcggg
    11941 cgttgcgctt cgaaaagcgg ctggcgcccg agctcaacgt ggaattggcc gccgtgccct
    12001 actaccggca gacgctcgat ttcacctgcg gtccgtcggc gctgatgatg gcgatgaaga
    12061 cactgcagcc cgaactgccg ctcgaccgcc ggctcgaact caatctgtgg cgcgagtcga
    12121 ccaccatctt catgacctcg gggcatggcg gctgctcgcc ctatggcttg gcactggcgg
    12181 cccaccaccg cggtttcgat gtcgagatcc atgtcaagga tgatgccgcg ctgttcgtcg
    12241 attcggtgcg cgatccggag aaaaaggcgg tgatccgcct ggtgcaggag gccttcatcg
    12301 ccgagctgag ccagttgccg atcccgatcc actacggtgt cgtcaccatc gaggagctgg
    12361 aagcggcgcg gcgacgcggc ggggtgccgg tggtgctgat cagctcctac cgcatctatg
    12421 gcgagaagtt tccgcactgg gtggtggtga ccggcttcga tgagcgctgc atctatgtcc
    12481 atgatcccta tctggatgaa aattccgaca gggtcatcat ggactgcatc aacatgccga
    12541 tcctgcgccg cgacttcgcc cgcatgaacc gctacggcaa ggcggcgcag atggcgacgc
    12601 tggtagtcta tccgcgaccg catggcggcg cgatcgaagg caaaccatca gccacaccgt
    12661 gacgacccat tttcgaggtg acgatgtccc gcgcgatcat tctggttgaa gagttggagg
    12721 attggcccgc cacctttccc gccgtggagc tggtgcgggc gcgcgactac cttgccagca
    12781 gcgccctgca ggagacgcgc gcgctgcggg tgatcaacct ctgccgcggc taccactacc
    12841 tgacgctggg ctactactgc tcgctgctgg gcgaggcgcg ccggcaccgg ctgctgccct
    12901 cggtgcgcac gctggccgac ctcgaccgca agaacctcta cgagctggtc accctggagc
    12961 tgaaccagga gatgctgcgt ccggcgatgg agagtgccgc ggccgaggag ctgtcgttca
    13021 acatctactt tggcaagagc gagcgccccg gcttcacctg gctggcgcgc gcgctctatg
    13081 aggcctttcc ggcaccgatc ctgcgggtca atctgcatct gggcgaaaag tcggaggtgc
    13141 gcgccatcga ggccggtgcg ctgcatgagc tcgatgacgc gcagcagcgg ctctttctcg
    13201 ccgcactcga ggcgctgctc aaccgcggct ggcggcgacc gcgcgcccgg cgccacttcc
    13261 gctacgacat ggcgattctg cacgacccgg ccgagaagct ggccccctcc aacccgcgcg
    13321 cgctggcgcg gatgattcgt gccggacggc ggctcggcat caatgtcgaa ctggtcaccc
    13381 gccgcgacta tgaccggctg gccgaatatg acgcgctctt catccgcgag accacccaca
    13441 tcgatcacca cacctaccag ttcgcccgca aggcggagcg tgaaggcatg gtggtgatcg
    13501 atgactccga ctcgatcgtg cgctgcacca acaaggtcta tctggccgag ctgatcgatg
    13561 cccatgaact ggccgcgccc accaccgtga tcatcaaccg cgacgagatc gaccgggtcg
    13621 agcagcagct cgactacccg atggtgctga agattcccga tggctccttc tcgcgcggcg
    13681 tgcacaaggt gcaaaaccgg accgagctgc atcaggtggc cgcgcagctc ttcgccagca
    13741 gtgacctgat tctggcccag gcgttcgtct acaccgagtt tgactggcgc atcggcgtgc
    13801 tcaaccgcaa gccgctgttc gcctgccagt acttcatgtc gaaacaccac tggcagatcg
    13861 tcaagcatga gggcgagggc cgtttcaagg tcggcaacca ccgcgcggtg ccggtcgcgg
    13921 aggtgcccga agcggtgatc cagctcgcgc tgaaggcagc caacctgatc ggcaatggcc
    13981 tctatggcgt cgacatcaag cagagcggca atcgcctcta tctgatcgag atcaacgaca
    14041 acccgaacct cgatgccggc gtcgaggatc agattctggg catggcgctc tatcagcagt
    14101 tgatgcagga
//
LOCUS       DAORCQ010000063        13674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2893_length_13674_cov_6.339599, whole genome shotgun sequence.
ACCESSION   DAORCQ010000063 DAORCQ010000000
VERSION     DAORCQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13674
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2893_length_13674_cov_6.339599"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..497)
                     /locus_tag="PKI23_07750"
     CDS             complement(174..497)
                     /locus_tag="PKI23_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36869.1"
                     /translation="MLDQPIRHPQQQDTPGEQQRQHERRQFFQKKIEWLKSTCLSELN
                     GQESELATQQQQNAEIRPDLIDLDHRHGNDQDFLNGVRAREPASPCAALHVQPRRAKG
                     TAYAP"
     gene            474..3539
                     /locus_tag="PKI23_07755"
     CDS             474..3539
                     /locus_tag="PKI23_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNN36870.1"
                     /translation="MPDRLIEHPLDPLRCATLVTLVVAVLRHRLELRGLTLIAATIAG
                     LIHPLTTLGFAGRGDWLSGWAPLDLTGAGMLYTLGGWLILAALWAFDLRKEAGHRLAP
                     VTADRLPAMAAGGLLLAAGCIGLNISAGSAQISQLIDTVNNSLLAGVVGTLVAALSVP
                     QRRQQIRWMISMQGGLAGMISVSGSGHRLVPLEALFLGGFAAATMLMINHWLRRSRLD
                     DPLCSVAVLLGGGTVGLITAGLPGVPSSPWTDREFWLQITLQLFGVTVIALVGAGLSW
                     LLFSQFRRWRLVEMKQPDVTVEVSPRMERLLAEVDQHGRQGYPDRRLKVEEGDEVGLL
                     AGKYNEVMERLESSMDFARLLVRDLQEGLITCSTAGQVLQMNPAAERLFAMAAADVQG
                     ASILNFLAFDQSGVDKRVLLHDGFRQDLTLLRGDGDRTRVLVSVTESRRGQHGRFLTV
                     LMQDVQTQWQVREQLQQESNFAQLVLESIIDSMITLNLHGRVTYLNQAAGRLLGWSAG
                     DATGLPLARLISSAEDQAGRHIADLAEYVLRLVAQQSRGEVLVLCNRYGDEYHLGCSL
                     APILNRADEQVGVVVLLHDVTQDRQFAQLLSYQATHDTLTGLYNRHAFEAELSSLLEE
                     SVATRPRHVLCLGDLDQFKVINDSCGHAAGDELLRQLTSLMRSKVRERDMLARVGGDE
                     FGLLLQNCEIERAEKVVADLIATVADYRFVWHANTYAVGISFGITPIDPAHGDTIQIM
                     NNADAACFAAKDAGRNRFHRYEPSDEELSRRMGEMSWVSRIQQALDADQFELYCQEIR
                     PLSDDLKPHYEVLVRLRTEDGKLVGPGAFVPAAERYGLIQRIDRWVVSKVLEWLKREE
                     ARLGDIRLAVNLSGFSVGDETFHAALLAQISQVEVARQRICFEITETAAITSFNHAVE
                     FLNRFKALGFRFALDDFGSGVSSFAYLHRLPIDLVKIDGIFVRELLADEMKQSIVTAI
                     VQISAQAGLLTIAEFVEDDATLAWLRAAGVNYAQGNGVSMPFPLDELLA"
     gene            complement(3560..3886)
                     /gene="yciH"
                     /locus_tag="PKI23_07760"
     CDS             complement(3560..3886)
                     /gene="yciH"
                     /locus_tag="PKI23_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HNN36871.1"
                     /translation="MERNSRPVYSTQSGRIREQKPQAPAAPSSDGVVRVRRERKGRGG
                     KTVTVVQGVPLDETGLKALAQQLKQRCGSGGTLKDGVIEIQGDHLELLLGLLGEAGFK
                     VKAAGG"
     gene            complement(3907..4788)
                     /locus_tag="PKI23_07765"
     CDS             complement(3907..4788)
                     /locus_tag="PKI23_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN36872.1"
                     /translation="MSDMRFDGKVAIVTGAGGGLGRAHALLLASRGAKVVVNDLGGAV
                     DGSGSSQTPAQKVVEEIKALGGQAVADANTVATQAGGKAIVDAALKAFGAVHIIVNNA
                     GILRDKSFRNMSPELFDPVIDVHLKGCVWTTHAAWELMREQNFGRVINTSSAAGLFGN
                     FGQTNYSAAKMGIVGLTKTLALEGAKYNIKCNVLAPGARTRMTEDLMGERAAAMDPEL
                     VAHTVAYLAHESCSLTGEILSSSSGRVARVFVGACKGVTNREQMSPEFIRDNLAQIMS
                     TDGFHIPAGAMDEISQR"
     gene            complement(4912..5799)
                     /locus_tag="PKI23_07770"
     CDS             complement(4912..5799)
                     /locus_tag="PKI23_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNN36873.1"
                     /translation="MTTATQSHQTATALLDFYANNGWANRVGIQLLEATPAAVSLFLG
                     YDERNLNAPGGVVHGGVIGTLIHDAGQLLSKLHFAQTPIRQIRPLDIQINYMIGAKEA
                     DLTATATLIRATRALAFIDVEVQNQNRQCVARAHTLFRIAPADEEAVELDSALLHELA
                     VKSPLALNQIGEMIKGNVARHAEGLQVLGLDQSRVRFTLDDLASNRDHEGALAGGAVL
                     LLLDDAGVFCSFGQIQRGRAATVDLKVNFCQSLADEQVIAFGHCLKRQSEAITNQVLL
                     FGASSGRLGAIGTMTFWGS"
     gene            complement(5875..6042)
                     /locus_tag="PKI23_07775"
     CDS             complement(5875..6042)
                     /locus_tag="PKI23_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36874.1"
                     /translation="MVNMVNLHGTLIWMHSCRLQGRQRGQFAITPLATGSAANHCKQR
                     QGCRLQWRSEW"
     gene            6080..7012
                     /locus_tag="PKI23_07780"
     CDS             6080..7012
                     /locus_tag="PKI23_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNN36875.1"
                     /translation="MQFKDYYQILGLSRDATQEEIKRAYRKLARKYHPDVSKAEDAEA
                     RFKEVGEAYEVLKDPEKRAAYDRFGEHWREGQDFTPPPNWDSGFEFTGGGFTDHDPQG
                     FSDFFEQLFGRAGPRRAGRGRAARHAEDHHARITITLEEAYQGASREVVLQRPTINRA
                     GHLEMQPHALRLRIPKGVREGQKIRLCGQGEPGNPAGDLYLEVCFAPHRLYRVDGADI
                     HLELPITPWEAALGARVKVPTLGGVVELAIPEGSQSGRRLRLKGRGLPASPAGDQYVT
                     LKVVVPPASSQAARELYRAMEKTIPCSPRSELGV"
     gene            7015..>7280
                     /locus_tag="PKI23_07785"
     CDS             7015..>7280
                     /locus_tag="PKI23_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HNN36876.1"
                     /translation="MATQDDEKQVAVLIEHHQYRLGELCQQLDVTAEWVMAMVGEAIV
                     EPTGAPISWCFSHSDLERLRKARNLERELQINLPGIALALDLLA"
     assembly_gap    7281..7380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7698..8387)
                     /locus_tag="PKI23_07790"
     CDS             complement(7698..8387)
                     /locus_tag="PKI23_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNN36877.1"
                     /translation="MNSTDHAALLAPSYFIDSDHPAVVEFAMRAIDGAQESRERAVRL
                     YYAVRDQIRYNPYAADMSREAMKASTVAQRGESFCVPKSILLAACARAVGIPSRLGFA
                     DVRNHLTSNRLKESMRGNDLFVFHGQTLMYVDGRWSKSTPAFNLSLCQKINILPLEYD
                     GQTDSVFHPFDQAGNRHMEYVRDRGLYDDMPYEAFATAMQQTYGKTFAESAGVRDGSA
                     DDFEAEAYAGS"
     gene            8674..9870
                     /locus_tag="PKI23_07795"
     CDS             8674..9870
                     /locus_tag="PKI23_07795"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNN36878.1"
                     /translation="MDQLFDDVRILDLSEGIPGPFASRIFADYGADVIKVESPGGDPA
                     RRHGPFPGDRPDPEASALFLHLNRNKRGITLDITSASGAELLRKLVKSADIVVESFAP
                     GHLAACGLGFERLREIKPDLVLASLTPFGQDGPYAGYQGNDIIYYALGGMNMTGDAEL
                     YPIKKAASMVEHQVGNNLALAMAAGFMAARYQGRGQHIDLAAAEVQQTSVDRRAQLLL
                     GHAYSGESSSRESLSAGALPSGIFPCRDGYVQVMTTPAWAERMARTLNRPDFMKRLAE
                     PGALYSMEFKAEIDAVFQPWLAAHTRHELFQQALANQWPVYPINTIKDLMEDAHFRSR
                     EFWIEQEHPVVGPLTYPGPLARMESGFALRWPSPRLGEHNQAIYGTELGLSGAELSRL
                     KAAGVI"
     gene            9890..11224
                     /locus_tag="PKI23_07800"
     CDS             9890..11224
                     /locus_tag="PKI23_07800"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNN36879.1"
                     /translation="MNSALPLSGIRVLDLTVVWAGPAATQHLADLGAEVIRIESAHRV
                     PSSTRAQPHVTADMVKRMGMLSRMSYPGGEPQPQPYNRTSSFNVLNRNKLSATMELDT
                     PEGKEGFRRLVEISDILVENNAQRIGDRLGFSWKELSAINPKLILCRMPPLGLTGPYA
                     NYLGFGAHFEALSGLTWLRAYPDADPSRTSPTFHMDDVSPTVAAFMILGALHRRRRTG
                     EGGLIEIPQAETMLHQIGDVVLDYSMNNRTLPPPGNRHHLYAPQGCYRCQGDDQWVVL
                     SVRDDGDWQRLVQAIGAPEWARDPAYATVNGRHAHHDQIDHHLNGWTHSRNKLEVFHL
                     LQEHGVPAAPVQNEADTFADPQVNHRGLLRPLRQESTGVYNHPRQAYLFSDMELRWER
                     GAPTLGEDNTYVYKELLGYSEQAYRDLEARGLIGTNYAVERPGGPGSAEAGD"
     gene            11272..11775
                     /locus_tag="PKI23_07805"
     CDS             11272..11775
                     /locus_tag="PKI23_07805"
                     /inference="COORDINATES: protein motif:HMM:NF040504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNN36880.1"
                     /translation="MANLNSQIRPVAQGDGAALADIYNHYIANTLITFEEQPLDASDM
                     TGRIDEVAAAALPWLVAERQGQIVGYAHASRWKGRCAYRFTVESTVYLDPVAVGQGVG
                     SQLYRALLDRLRDRAIHVAIAGIALPNPASVALHERFGFRKVGHFQEVGFKFDRWIDV
                     GYWQVLL"
     gene            11776..11925
                     /locus_tag="PKI23_07810"
     CDS             11776..11925
                     /locus_tag="PKI23_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36881.1"
                     /translation="MDAALSAPTQMTRTAIDSALLLRNIAAEPVPIHVQQGFRHEKIR
                     FIDRP"
     gene            11894..12970
                     /locus_tag="PKI23_07815"
     CDS             11894..12970
                     /locus_tag="PKI23_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36882.1"
                     /translation="MKRSASSTALDEAYRSEVKRSTFLDPRQNFAPHSVETEFRYDPL
                     TGQSSRICHFSRDKMPAPDLSRMVEESKGFCPFCPGLIEKVTPHYPPELLAEGFLTCG
                     SARLFPNLFPYDDVSAVAAITDQHFVPMDDFPVEVIANGLNVARAFFERLEAAPTPRE
                     VPFFNMVTWNYMPPSGSSLIHPHMQVLSTSNPGNALRRELAAEAAYFERSGRPFHADL
                     LDHERRHDKRWIGEHGGVQWLVPYAPVGMIGDCQAIFPGKATLSDLSADEILYFARGL
                     HTLLKCFADYGLWSFNLAFFPAAIGSAPDRHWLNARVVPRIFVNPMTSAADASYLQFV
                     IEERVAMVYPEVTAMRLREGWNLG"
     gene            12975..>13674
                     /locus_tag="PKI23_07820"
     CDS             12975..>13674
                     /locus_tag="PKI23_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNN36883.1"
                     /translation="MTPPDDALDTLRAWLLDSRSTVIFTGAGISTESGIPDFRSPGGL
                     WGRYRPIDFGEFMASEAKRRESWRMRFALENEELFGQAQPNIAHRRIAEWVEQGRVGC
                     VITQNIDGLHQAAGVPGAQLIELHGNNQHACCLECHKRFELAPIRANFMRDESVPYCD
                     RCGGIVKSAVISFGQPMPEAQMRRAQAVSLGCELFIVIGSSLLVHPAADLPALAKGRG
                     ARLVIINREPTPLDQ"
BASE COUNT         2404 a         4264 c         4373 g         2533 t
ORIGIN      
        1 ctgacttctc ctgtcaggcg cagccaagcg gtactggttt ttgttccgtg tgccccggcc
       61 gaatgccggg gcttttttgt gatgggatcc tgactgatcg accaacggat cggtctgaag
      121 ggctgatcga attttgtgcc ttgtcctgtt acagttccca ggcaggcggg cgctcacgga
      181 gcataggctg ttcccttcgc tcttcgtggt tgaacgtgca gggcggcaca tgggctggcg
      241 ggttctctcg ccctgacacc attcaagaaa tcctggtcat ttccatggcg atggtcgaga
      301 tcgatcaagt ctggtcgaat ttctgcattt tgctgctgct gtgtggccag ctcggactcc
      361 tgtccattca gttcggacag gcacgtcgac ttcagccatt cgatcttttt ctggaaaaac
      421 tggcggcgct cgtgctgcct ttgctgctct cctggggtgt cttgctgctg tggatgcctg
      481 atcggctgat cgagcatcca ctcgatccgt tgcgctgtgc cacgctcgtc acgctggtgg
      541 tggctgtgct gcgtcatcga ctcgaactgc ggggattgac actgattgcc gcgacgatcg
      601 ccgggttgat ccacccgctg acgaccctgg ggttcgctgg ccgaggagac tggctgagcg
      661 ggtgggctcc gctcgatctg accggggcgg gcatgctcta cacgctgggg ggttggttga
      721 ttctggcggc tctgtgggcg ttcgatctgc gcaaggaggc gggacatcgc ctcgcgccag
      781 tcaccgccga ccggctgccc gccatggccg ccggaggtct actgctggcg gcaggctgca
      841 ttggattgaa catcagcgcc gggagtgcac agatcagtca gttgatcgac accgtcaaca
      901 acagcctgct ggctggcgtg gtgggcacat tggtggctgc cctctcggtg ccacagcgtc
      961 gccaacagat ccgctggatg atttcaatgc agggtggcct ggcaggcatg atctcggtca
     1021 gcggcagcgg tcatcggctg gtcccgctcg aagcactctt tctgggcgga tttgccgccg
     1081 ccaccatgct gatgatcaac cactggctgc gtcgatcgcg gttggacgat ccgctgtgca
     1141 gtgtggcagt gctgctgggg ggcggtacgg tgggtctgat cactgcgggt ctgcccggag
     1201 tgccttcttc gccatggacc gaccgcgaat tctggcttca gatcaccctg caactgtttg
     1261 gtgtcactgt gattgccctg gtgggagcag gactctcctg gctgctgttt tcacaatttc
     1321 ggcgctggcg gttggtggag atgaaacagc ccgatgtcac cgtggaggtc agcccgcgga
     1381 tggagcggct gctggccgag gtcgatcagc acggtcggca gggatatccg gatcgacgcc
     1441 tgaaggtcga agaaggtgac gaggttggac tgctggccgg aaaatacaac gaggtgatgg
     1501 agcgcttgga gagctccatg gattttgcgc ggctgttggt gcgggatctt caggaaggtc
     1561 tgatcacctg ttcgaccgca ggtcaggtgt tgcagatgaa tccggcggcc gaacggctgt
     1621 tcgccatggc ggcggccgat gtgcaggggg cttcgatcct gaattttctg gcttttgacc
     1681 agagcggcgt ggacaaacgg gtgctgctgc acgacggctt tcggcaggat ctgaccctgc
     1741 tgcggggcga tggcgaccgg acgcgggtgc tggtcagcgt gaccgaatcc agaagagggc
     1801 agcatggccg tttcctgacc gtgctgatgc aggatgtgca gacacagtgg caggtcaggg
     1861 agcagttaca gcaggagagc aatttcgccc agttggtgct ggagtcgatc atcgacagca
     1921 tgatcaccct caacctgcac ggccgggtga cctatctgaa ccaggcggcc ggacgcctgc
     1981 tcggctggtc ggcaggcgat gcgacggggt tgccgctggc gcggctgatc tcctcggcgg
     2041 aagaccaggc gggccgccac attgccgatc tggccgagta tgtgctgcgg ttggtggcgc
     2101 agcagagccg tggcgaggtg ctggtgctgt gcaaccgcta tggcgatgag taccaccttg
     2161 gctgctccct ggcaccgatt ctcaaccgtg ccgatgaaca ggtcggggtg gtggtgctgc
     2221 tgcacgacgt gacccaggac cggcagttcg cgcagttgct gagctatcag gccacccatg
     2281 acaccctgac cggtctctac aaccgccatg ccttcgaggc ggaactgagc tcgctgctcg
     2341 aagagagcgt cgccacccgt ccgcggcatg tgctctgtct gggtgatctc gaccaattca
     2401 aggtgatcaa tgacagttgc ggtcatgcgg ccggcgatga actgctgcgc cagctcacca
     2461 gcctgatgcg ctccaaggtg cgtgaacgtg acatgctggc gcgggtgggt ggcgacgagt
     2521 tcgggctgct gctgcagaat tgcgagatcg aacgcgccga gaaggtggtg gcggatctga
     2581 tcgcgaccgt ggccgactac cgcttcgtct ggcacgccaa cacctacgcg gtgggcatca
     2641 gctttggcat caccccgatc gatccggcgc atggcgatac gatccagatc atgaacaatg
     2701 ccgacgcggc ctgctttgcc gccaaggatg ccggccgcaa ccgctttcac cgctatgagc
     2761 cgagcgatga ggagctgagc cggcgaatgg gtgaaatgag ctgggtcagc cgcatccagc
     2821 aggcgctcga cgccgaccag ttcgagctct actgccagga gatccggccg ctctccgacg
     2881 acctcaagcc gcactatgaa gtgctggtac ggctgcgcac cgaggatgga aaactggtgg
     2941 gaccgggtgc cttcgtgccg gcggcggagc gctacgggct gattcagcgc atcgaccgct
     3001 gggtggtgag caaggtgctg gagtggctga agcgtgaaga ggcgcggctc ggcgacatcc
     3061 gccttgcggt caacctctcc ggcttttcgg tgggtgacga gaccttccac gccgcactgc
     3121 tggcacagat cagccaggtc gaggtggccc ggcagcgcat ctgcttcgag atcaccgaga
     3181 ccgcggccat caccagcttc aaccatgcgg ttgaatttct gaatcgattc aaggcattgg
     3241 gatttcgttt cgcgctcgat gactttggca gcggcgtctc atcctttgcc tacctgcatc
     3301 ggctgccgat cgatctggtc aagatcgatg gcatcttcgt ccgcgaactg ctggcggacg
     3361 agatgaagca gtcgatcgtc accgccatcg tgcagatctc tgcccaggcc ggtctgctga
     3421 ccattgccga attcgtcgag gatgacgcca cattggcatg gctgagagcg gctggcgtca
     3481 actatgccca agggaatggc gtgagcatgc cattcccact tgacgaactg ctggcctgac
     3541 cggttgctct gcagacggtt cagccgccgg cggccttgac cttgaagcca gcctcgccaa
     3601 gcaggcccag cagcagttcg aggtggtcac cctgaatctc gatgacgcca tccttcaggg
     3661 tgccaccgct gccgcagcgc tgcttcagtt gctgcgccag cgccttcagt ccggtttcat
     3721 ccagcggaac cccctgcacg acggtgaccg ttttgccacc gcgtcccttg cgttcacgcc
     3781 gaacccgcac cacgccgtcg ctgctgggcg cggcgggcgc ctggggtttc tgctcgcgga
     3841 tgcgcccgct ctgcgtggag tagaccgggc gtgagttgcg ctccatggtg gcggaactgt
     3901 cggggctcag cgctggctga tctcgtccat cgccccggcc gggatatgga agccatcggt
     3961 gctcatgatc tgggccagat tgtcacggat gaactccggg ctcatctgtt cgcggttggt
     4021 gacgcccttg caggcgccga caaaaacccg cgccacgcgg ccggaggagg aggagagaat
     4081 ctcgccggtc aggctgcacg actcatgggc cagataggcg acggtgtgcg cgaccagttc
     4141 ggggtccatg gcggctgccc gctctcccat cagatcctcg gtcatgcggg tgcgtgcgcc
     4201 gggcgccagc acgttgcact tgatgttgta tttggcacct tccagggcca gcgtcttggt
     4261 gagaccgacg atgcccatct tggccgccga gtagttggtc tgtccgaagt tgccgaacag
     4321 gccggcggcc gacgaggtgt tgatgacgcg gccgaagttc tgctcgcgca tcagctccca
     4381 ggcggcatgg gtggtccaga cgcagccctt gaggtgcacg tcgatcaccg ggtcgaacag
     4441 ctccggtgac atgttgcgga aggatttgtc gcgcaggatg ccggcattgt tgacgatgat
     4501 gtggaccgcg ccaaaggcct tgagcgcggc atcgacgatc gccttgccgc cggcctgggt
     4561 ggcgacggtg ttggcatcag ccaccgcttg gccgcccagc gccttgatct cctcgaccac
     4621 cttctgggcg ggggtctgtg acgaaccgct gccgtcgacc gcgccgccga ggtcattgac
     4681 caccaccttg gcgccacggg aggccagcag cagcgcatgg gcgcggccga gaccgccgcc
     4741 ggcaccggtg actatggcga ctttgccatc gaatcgcatg tctgacatgg gaacgctcct
     4801 tgattgttat ctctgttatg ggggcaggcg gctggcgccc gccggtctaa tctacccaca
     4861 ccgtgaggga gggtaaaggg cacacggtga tgcctgcctg caacgggcgc ctcaagagcc
     4921 ccagaaggtc atggtgccga tggcgccgag ccggccgctg ctggcaccga acagcagcac
     4981 ctggttggtg atcgcctcgc tctggcgctt caggcagtgg ccaaaggcga tcacctgctc
     5041 gtcggcgaga ctctggcaga agttcacctt cagatcgacg gtggcggcgc gaccgcgctg
     5101 aatctggcca aaggagcaga acaccccggc atcatcgagc agcagcagca cagcgccgcc
     5161 agccagtgcg ccctcatggt cacggttgga ggcgagatca tcgagcgtga agcgcacccg
     5221 gctctggtcg agccccagca cctgcaaccc ctcggcatgg cgggcgacat tgcccttgat
     5281 catctctccg atctgattca gcgccagcgg acttttcacc gccagctcat gcagcagcgc
     5341 gctgtcgagt tcgaccgcct cttcatccgc cggggcgatg cggaacagcg tgtgggcgcg
     5401 ggcgacacac tgccggttct ggttctgcac ctcgacatcg atgaaggcca gcgcccgggt
     5461 cgcacggatc agggtcgcgg tggcggtgag gtcggcctcc ttggcaccga tcatgtaatt
     5521 gatctggatg tcgagcgggc gaatctgcct gatcggggtc tgggcgaagt ggagcttgct
     5581 cagcaactgg ccggcgtcat gaatcagggt gccgatcacg ccgccatgga ccacgccacc
     5641 gggggcgttg aggttgcgct cgtcatagcc gagaaacagg ctgactgcgg cgggcgtcgc
     5701 ttcgagcagc tggatgccca cccggtttgc ccagccatta ttggcataga agtcgagcag
     5761 ggcggtggcg gtctgatggg attgggtcgc agtggtcatc tccctattgt ctccttcaag
     5821 gggttgctgg atggctggat tattgttgtt tgcgccgggc caaacatccc attgtcacca
     5881 ttcgctgcgc cattgcaatc ggcagccctg ccgctgtttg cagtggtttg cggcagagcc
     5941 ggtggcaagc ggcgtgatgg cgaactggcc gcgctggcga ccttgtagcc ggcacgaatg
     6001 catccatatt aaagtaccgt gcagattgac catgttgacc atgaatcacc cgtccctcac
     6061 cgcgaagtga cgctgaacca tgcaattcaa ggattattac cagattctcg gcctctcccg
     6121 tgatgccacc caggaggaga tcaagcgcgc ctaccgcaag ctggcgcgca agtaccatcc
     6181 cgacgtcagc aaggccgagg atgccgaggc acggttcaag gaggtcggcg aagcctacga
     6241 ggtgctgaag gatccggaaa agcgggccgc ctatgaccgt tttggtgaac attggcgtga
     6301 aggccaggat ttcaccccgc cgcccaattg ggacagcggc ttcgagttca ccggtggtgg
     6361 ctttaccgac catgatccgc aaggtttcag cgacttcttc gaacagctgt tcggccgagc
     6421 tggacctcgt cgcgctggcc ggggtcgagc cgcgcgccac gccgaggatc accacgccag
     6481 gatcaccatt acccttgaag aggcctatca gggggcgagc agggaggtgg tgctgcaacg
     6541 gcctaccatc aaccgtgccg gccacctgga gatgcagcct catgcgctgc ggctgcgcat
     6601 tcccaagggg gttcgcgagg ggcagaagat tcggctctgt ggccagggtg agcccggcaa
     6661 tccggccggt gacctctacc tcgaagtctg ctttgccccc catcggctct atcgggtgga
     6721 tggggccgac atccatctgg aactgccgat caccccgtgg gaggccgcgc tcggtgccag
     6781 ggtcaaggtg ccgacgctgg gtggagtggt tgagctggcg atccccgaag ggtcgcaaag
     6841 cgggcggagg ttgcggctga aggggcgcgg tctgcctgcc tcgccggcgg gtgaccagta
     6901 tgtgaccctg aaggtggtgg tgccgcccgc cagcagtcag gcggcgcgtg aactctaccg
     6961 cgcgatggag aagacgatac cttgcagccc gcgcagcgaa ctgggggtct gagcatggcg
     7021 acacaggatg atgaaaaaca ggtggccgtg ctgatcgagc accatcagta ccgcctgggt
     7081 gagctgtgcc agcaactcga tgtcaccgcc gagtgggtga tggcgatggt cggcgaagcg
     7141 atcgttgagc cgacaggagc gccgatcagc tggtgtttca gccacagtga cctcgaacgg
     7201 ctgcgcaagg cgcgcaatct cgaacgcgag ttgcagatca atctgcccgg cattgcgttg
     7261 gcgcttgacc tgctggctga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 aaaaattttg cgcatgctga ctgtgaattg caagaaacac tggttatttc aggcttgatc
     7441 atctcaactc attgtaaata aacaactatt ttattgatca aaaaacaacc taagaaaaaa
     7501 atgcctgctg tttcttgagt tagcgttgga cgcggctgaa ttatccagaa agttatccac
     7561 agaaaatgtg catcagaaat gacatctgca tgacgttttc atggcgggag agatgtcatt
     7621 gagagacggg gcaaaaagca tcgcgcgccg caatggcgcg ctccagttga aaggatgagc
     7681 gtgaggcgag ctggccctca actgccggcg taggcctcgg cctcgaagtc gtcggcgctg
     7741 ccatcgcgca cgccggcact ctcggcaaag gtcttgccgt aggtctgctg catcgcagtg
     7801 gcaaaggctt cgtagggcat gtcgtcatac aggccgcggt cgcgcacata ctccatgtgg
     7861 cggttgccgg cctgatcgaa ggggtgaaac accgagtcgg tctggccgtc atactccagc
     7921 ggcaggatgt tgatcttctg gcagagcgaa agattgaagg ccggcgtgct cttgctccag
     7981 cggccatcga catacatcag ggtctgaccg tggaagacga acagatcgtt gccgcgcatc
     8041 gactccttga gccgattgga ggtgaggtgg ttgcgcacgt cggcaaagcc gagccggctt
     8101 ggaatgccca ccgcccgcgc gcaggccgcg agcagaatcg atttgggcac gcagaaggat
     8161 tcaccccgtt gcgccaccgt gctggccttc atcgcttccc ggctcatgtc ggccgcgtag
     8221 gggttatagc ggatctgatc acgcacggca tagtagaggc gcaccgcgcg ctcccggctc
     8281 tcttgggcgc cgtcgatggc gcgcatggcg aactccacca ccgccggatg gtcgctgtcg
     8341 atgaagtagc tgggtgccag cagcgcggcg tggtcggtcg agttcatgcg ggttctcctg
     8401 tgggcaaatg cgggtccgtt gcggcaagca tagccagagc gacgaaaacc gacaaccggc
     8461 ggaatcccgt cgaccttggg tgcttgccag cggggcaagg cttgacagcg gccccggcaa
     8521 ttgggcagtc tggccatgga ttcacagcag ccggccgcgg gtcggcacga taataaaaga
     8581 gtccatcatc accgcatggg cagtgcccca tcggtaccct gaacaccgct tgcgacttca
     8641 gacccgccgt ctgaagtcga tgaggagttg cgcatggacc agctgtttga cgacgttcgc
     8701 attctcgatc tctccgaggg gatccccggt ccctttgcca gccgcatctt tgccgactac
     8761 ggcgccgacg tcatcaaggt cgaatcaccc ggcggtgacc cggcacggcg gcatggcccc
     8821 tttccgggtg accgccccga tccagaggcc agtgcactct ttctgcatct caaccgcaac
     8881 aagcgtggca tcacgctcga catcaccagt gccagtggtg cggagctgct gcgcaaactg
     8941 gtgaagagtg ccgacattgt ggtggagagc ttcgcgcccg gccatctggc cgcctgcggt
     9001 ctcggcttcg agaggctgcg tgagatcaag cccgatctgg tgctggccag cctgaccccc
     9061 tttggccagg atggccccta tgccggctat cagggcaacg acatcatcta ctacgccctg
     9121 ggcggcatga acatgaccgg ggatgccgag ctctatccga tcaagaaggc cgcctcgatg
     9181 gtcgagcatc aggtgggcaa caatctggcg ctggcgatgg cggccggttt catggctgcc
     9241 cgctatcagg ggcgtggcca gcacatcgat ctggccgcgg ccgaggtgca gcagaccagc
     9301 gtcgaccgtc gcgcccagtt gctgctgggc catgcctaca gcggagagtc gtcgagtcgc
     9361 gagtcgctct cggccggtgc gctgccctcg ggcatcttcc cctgccgcga tggctatgtg
     9421 caggtgatga ccaccccggc ctgggcggag cggatggcac gcaccctcaa ccggcccgac
     9481 ttcatgaaac ggctggccga accgggcgcg ctctacagca tggagttcaa ggccgagatc
     9541 gacgcggtgt tccaaccctg gctcgcggcc cacacccgcc atgagctgtt ccagcaggcg
     9601 ctggccaacc agtggccggt ctatccgatc aacaccatca aggatctgat ggaggatgcc
     9661 cacttccgca gccgtgaatt ctggatcgaa caggagcatc cggtggtcgg cccgctgacc
     9721 tatccgggcc ccttggcccg catggagagc ggctttgcgc tgcgctggcc atcgccacga
     9781 ctgggcgagc acaaccaggc catctacggc actgaactgg gtttgagcgg cgccgaactg
     9841 agccgcctca aggccgccgg cgtcatctga gagtcacgga gaacacaaca tgaacagtgc
     9901 attgcctctg agtggcatcc gggtcctcga tctgaccgtg gtctgggccg gaccggccgc
     9961 cacccagcat ctggccgacc tcggtgccga ggtgattcga atcgaatcgg cccaccgggt
    10021 gccctccagc acccgtgccc agccccatgt caccgcggac atggtgaagc gcatgggcat
    10081 gctgtcgcgc atgtcctacc ccggtggcga gccgcagccg caaccctaca accgcaccag
    10141 ctccttcaac gtgctcaacc gcaacaagct gagcgcgacc atggagctcg acacccccga
    10201 gggcaaggag ggctttcgcc ggctggtcga gatctccgac atcctggtcg agaacaacgc
    10261 ccagcgcatc ggcgaccggc tcggtttcag ctggaaggag ctcagtgcga tcaaccccaa
    10321 gctgatcctc tgccggatgc cacccctcgg cttgaccggc ccctatgcca actacctcgg
    10381 ctttggtgcc cacttcgagg cgctctccgg actcacctgg ctgcgcgcct accccgatgc
    10441 cgacccatcg cgcacctcgc ccacctttca catggacgat gtcagcccga cggtggcggc
    10501 cttcatgatc ctgggtgcgc tgcatcggcg gcggcgcacc ggcgagggcg gattgatcga
    10561 gattccccag gccgagacca tgctgcatca gatcggtgat gtggtgctcg actacagcat
    10621 gaataaccgc accctgccgc cgcccggcaa ccgccaccac ctctatgcac cgcagggctg
    10681 ctaccgctgc cagggtgacg accagtgggt ggtgctgagt gtgcgcgacg atggcgactg
    10741 gcagcggctg gtgcaggcta tcggcgcgcc ggagtgggcc cgcgatccgg cctacgccac
    10801 cgtcaatggc cgccatgccc accatgacca gatcgaccac catctgaatg gctggaccca
    10861 ctcccgcaac aagctcgaag tcttccacct gctgcaggag catggcgtgc cggccgcgcc
    10921 ggtgcagaac gaggccgaca ccttcgccga cccgcaggtc aaccaccgcg ggctgctgcg
    10981 tccattgcgc caggagagca ccggcgtcta caaccatcca cggcaggcct atctcttctc
    11041 cgacatggaa ctgcgctggg agcgcggtgc gccgacgctc ggtgaagaca acacctatgt
    11101 ctacaaggag ctgctcggct actcggagca ggcctaccgt gacctcgagg cgcgtggcct
    11161 gatcggcacc aactatgcgg tggagcgccc cggcggccca ggcagcgccg aagcgggcga
    11221 ctgactgcat gggcagcgtc gaagggtgca cgagcggctg accggcagca gatggcgaac
    11281 ctgaacagcc agatccggcc ggttgcgcag ggcgatggcg cagcccttgc cgacatctac
    11341 aaccactaca tcgccaacac gctcatcacc ttcgaggagc agccgcttga cgccagcgac
    11401 atgaccgggc gcatcgacga agtggcggcc gccgcgctgc cctggctggt ggccgaacgg
    11461 caggggcaga tcgtcggcta cgcccatgcc agccgctgga aagggcgttg cgcctaccgc
    11521 ttcaccgtcg agagcacggt ctacctcgac cctgtcgctg tcgggcaggg cgttggcagc
    11581 cagctttacc gagcgctgct cgaccggttg cgcgaccgcg ccatccatgt ggccattgcc
    11641 ggcatcgcac tgccgaaccc ggccagcgtg gccctgcatg agcggttcgg attccgcaag
    11701 gtcggccatt tccaggaggt cggcttcaag ttcgatcgat ggatcgatgt cggctactgg
    11761 caggtgctgc tgtgaatgga cgctgcgctc agcgcgccga cgcagatgac ccgcaccgcg
    11821 attgacagcg ctctgctgct tcgcaatatt gccgcagagc ctgtcccgat ccatgtgcaa
    11881 cagggattcc gccatgaaaa gatccgcttc atcgaccgcc cttgacgagg cctaccgcag
    11941 cgaggtcaaa cgcagcacct ttctcgatcc acggcagaac ttcgcgccgc acagcgtcga
    12001 gaccgagttc cgctacgatc cgctgaccgg gcagagcagc cgcatctgcc acttctcccg
    12061 cgacaagatg ccggcacccg acctcagcag gatggtcgag gagtccaagg gcttctgtcc
    12121 cttctgtcct ggcctgatcg agaaggtgac gccgcactat ccgccggagc tgctggcgga
    12181 gggctttctc acctgtggct cggcgcggct cttccccaac ctctttccct atgacgatgt
    12241 cagtgcggtg gccgccatca ccgaccagca cttcgtgccg atggatgatt ttccggtcga
    12301 ggtgatcgcc aacggcctca atgtcgcccg cgccttcttc gagcggctcg aagcagcgcc
    12361 gacaccgcgc gaggtgccct tcttcaacat ggtgacctgg aactacatgc ccccctcggg
    12421 cagttcactg atccatccgc acatgcaggt gctctccacc agcaatccgg gcaatgcgct
    12481 gcgccgcgaa ctggccgccg aggcggccta cttcgaacgc agcggccgac ccttccatgc
    12541 cgacctgctc gaccatgagc gccgtcacga caaacgctgg atcggcgagc atggcggcgt
    12601 gcagtggctg gtgccctatg cgccagtggg gatgatcggc gactgccagg cgatcttccc
    12661 cggcaaggcg acgctcagcg acctgagtgc cgatgagatc ctctactttg cccgcggtct
    12721 gcacacgctg ctcaaatgct ttgccgacta tggattgtgg agcttcaacc tcgccttctt
    12781 tccggccgcc atcggcagcg cgcccgatcg ccactggctc aatgcccggg tggtgccgcg
    12841 catcttcgtc aacccgatga ccagcgcggc cgatgcctcc tacctgcagt tcgtgatcga
    12901 ggagcgggtc gccatggtct accccgaggt caccgccatg cggctgcgcg aaggctggaa
    12961 cctcggatga accgatgacc ccgcccgacg acgcactcga tacactgcga gcatggctgc
    13021 tcgacagccg ctcgacggtc atcttcaccg gcgctggcat cagcaccgag tcgggcattc
    13081 ccgacttccg cagccccggc ggcctctggg gccgctaccg gccgatcgat ttcggcgagt
    13141 tcatggccag cgaggcgaag cggcgcgaat cgtggcggat gcgtttcgcg ctggaaaatg
    13201 aggagctgtt cggccaggcc cagcccaaca tcgcccatcg gcgcatcgcc gaatgggtcg
    13261 agcagggccg ggtcggatgt gtcatcaccc agaacatcga tggcctgcac caagccgccg
    13321 gcgtccccgg cgcgcagttg atcgagctgc acggcaacaa ccagcatgcc tgctgtctgg
    13381 agtgtcacaa gcggttcgaa ctggcgccga tccgcgccaa cttcatgcgc gacgagagcg
    13441 tgccctactg cgaccgctgc ggcggcatcg tcaaaagcgc ggtgatctcc ttcggccagc
    13501 cgatgcccga ggcgcagatg cgtcgggccc aggccgtcag cctcggctgc gagctgttca
    13561 tcgtcatcgg ctcatcactg ctggtccatc cggcggcgga tctgccggca ctggccaaag
    13621 ggcgcggcgc ccggctggtg atcatcaacc gcgaaccgac gccgctggat caac
//
LOCUS       DAORCQ010000064        13376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_2979_length_13376_cov_5.655056, whole genome shotgun sequence.
ACCESSION   DAORCQ010000064 DAORCQ010000000
VERSION     DAORCQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2979_length_13376_cov_5.655056"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1310
                     /locus_tag="PKI23_07825"
     CDS             189..1310
                     /locus_tag="PKI23_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNN36884.1"
                     /translation="MRIVADENIPLLDPLFAPLGEVVRVPGRAITPALLRDADLLLVR
                     SLTRVDAELVRGSSIKFVGSATIGVDHLDCEPLRQLGIEVANAPGCNARAVAEYLLSL
                     LLTLREAERLPPQPRVAVVGLGSVGQQLCALLRTLGWPLDCVDPYRPDLANSDLDAAM
                     QADVITLHTPLTRQGDHPTWHLFDAARLARLHDGQLLINSGRGAVIDHQALLARLQQP
                     RAPSVALDVWEGEPNIDPALLDRLVLGTPHIAGHSLEGKMRGAWQLRQAIGAWLELPP
                     LPPLDTFLPPPLLREVTLAAAADPLDLLLQVMRLLYDIRLDDWRLRRALAQDPSAATF
                     DRLRKHYPGRREFASLRLRLPAEAEATRALFAALGFAVV"
     gene            1528..2253
                     /locus_tag="PKI23_07830"
     CDS             1528..2253
                     /locus_tag="PKI23_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNN36885.1"
                     /translation="MGRVIRMLPLLGVLALALACARSAPAAGNEGQPIAGGAFVIDGD
                     TLTVAGQRIRLYGIDAPEFHQLCQRDGEPWRCGQAATVALTEFIGRRPVRCEPRDREP
                     DSFEQGRHRYGRVIAVCSVGGAELNRWLVAEGHALAYRHYSSDYVADETQAQRARKGV
                     WGSEFEPPWVWRHQSNGVSRRSTLAPSTTPPARTAAEAECGTKKRCAQMSSCAEARDH
                     LTRCDIKSLDGDGDGVPCESLCR"
     gene            2332..3282
                     /locus_tag="PKI23_07835"
     CDS             2332..3282
                     /locus_tag="PKI23_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN36886.1"
                     /translation="MPNLPAAPPHALIRWSHRVEPGLTLRGWRTPPSGKPLLHFVHGN
                     GFCGLSYWAMLRRLGSHYDLFLSDVQGHGDSDAGERFDGWQVTVERLFAVIERFAPEW
                     GEVPVHGVGHSFGGVLTLSLAALHPQLFSSIVVLDPVLFPPELMQAEIESRQRGESPM
                     ARDARLRTALWPSRAAAWRHLHQRGGYKGWSDEAFACFIDHALYANDDGQYRLKCPPQ
                     IEAAIYSSVPLDYWRLIPQVDCPGVILHGDSTMPFIAPAVARALQLNPGLQAEQVTGG
                     HCFMQEQPAATAERVAQWLQRLAPSAVDCAATARQALHTP"
     gene            complement(3251..4225)
                     /locus_tag="PKI23_07840"
     CDS             complement(3251..4225)
                     /locus_tag="PKI23_07840"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HNN36887.1"
                     /translation="MSALRWRPLGALLLLWLAGCTQWSPPAGTAASPTLELSATPFFP
                     QARYQCGPAALATLLVDAGVETTPDQLAPQVYLPQRAGSLQPELIAASRRAGRIPYRL
                     DPHWSAIEAELAAGRPVLVLQNLGFSFWPRWHYAVVIGHDARHEGVILRSGTERRQRL
                     PMRQFLYSWQQSDHWALVVLKPGELLASVDSTRYLAELAVWESQGRNTAALRGYLAAV
                     ERWPDQPTARLGAGNMLHARGDHAAAAQQYRALLRLRPEDPVALNNLADTLAQLGCRD
                     QALTLIERGLRQPALAPAVRDTMVATRAEISARPATAAATECAAPAAP"
     gene            complement(4222..4608)
                     /locus_tag="PKI23_07845"
     CDS             complement(4222..4608)
                     /locus_tag="PKI23_07845"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HNN36888.1"
                     /translation="MQSRLLKRSLIALLVTQSVALATVQPAAAALISTESALTLEQRQ
                     SRLAPLQQALARADVQAKLIEKGVDPQAAAARIANLSDAEIASIQQQIDQLPAGGDGL
                     FAVLGIVFIVLLILDFVGATNVFRSV"
     gene            complement(4686..5747)
                     /locus_tag="PKI23_07850"
     CDS             complement(4686..5747)
                     /locus_tag="PKI23_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNN36889.1"
                     /translation="MNGQRISGILAALAAALLFGASTPLAKLLLTRIDPWLLAALLYL
                     GSGVGLWLLRRLGRAEAVRLERGDWGWLAAAIAAGGVAAPLLLMFALSAMPATTAALL
                     LNAEAVLTVLLAWFLFREPFDRRIAAGMLTIVAGALLLSWPQRLQLATLWPTLALLAA
                     ALAWAIDNNLTRKIALADASFIAMSKGLIAGSTNLLLALQLRVDWPAPGVVATAALLG
                     LLCYGVSLTLFVVALRQLGAARTSAYFGLAPFWGALLAIALLGEPLTPQLLVAALLMA
                     VGVALHLTERHQHLHHHAPMSHRHRHHHALDGDPHHDHSHAEPVPPGTRHSHFHHHPA
                     LSHDHPHFPDAHHGHRHPD"
     gene            complement(5744..6169)
                     /gene="cadR"
                     /locus_tag="PKI23_07855"
     CDS             complement(5744..6169)
                     /gene="cadR"
                     /locus_tag="PKI23_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HNN36890.1"
                     /translation="MRIGELARATGVDVETIRYYEKSGLLPAPARHDNGYRAYDRSHL
                     ERLSFIRHCRALDIPLADVQRLLRFIEQPEADCGDIDQLIAAQLNRVQARIVSLQALA
                     LQLDTLRRRCGTARTARDCGILQELVAAAHGESCACHAS"
     gene            6290..6946
                     /locus_tag="PKI23_07860"
     CDS             6290..6946
                     /locus_tag="PKI23_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNN36891.1"
                     /translation="MSDNCCGPTPAVALTPRYRKALWVALLVNSLMFFVEVAGSLRSG
                     SSALLADAIDFAGDAANYGLSLAVLSLALVWRSRAAWIKGATMALFGLLVLGRTGWAA
                     LHGLLPETWTMGLVALLALAANAGVALLLYAFRDGDANMRSVWLCSRNDAIGNLAVIL
                     AAAGVALTGSRWPDLLVAVALALLALSSGVSIMRHARDELNSALPSAVQSCQSDPCGR
                     "
     gene            complement(6957..8129)
                     /locus_tag="PKI23_07865"
     CDS             complement(6957..8129)
                     /locus_tag="PKI23_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNN36892.1"
                     /translation="MSDRRFAARVAEIEPFRVMAVLEQARTLEQAGRDIIHMEVGEPD
                     FPTAAPIVEAGRAALAAGRTQYSPALGLPELRAAISDHYRRTQGLDIDPRRILITAGA
                     SGALLLAAALLVEPGSGVLLADPGYPCNRHFIRLFGGEPQAVAVAAAQRFQLLPALAQ
                     QQWRPNSRGLLLATPSNPTGTVLNRSDLRGFAELAVQKEAFVLVDEIYQGLTYGSDAP
                     SMLAFTDEAYVINSFSKYFGMTGWRLGWLVAPPTAVAPLEKLAQNLFIAPSTPAQHAA
                     LAAFSASTIELLEAQRGEFQRRRDLLLPALRQAGLVIEREPEGAFYLYAAIDHLGLDS
                     ETFCLRLLEEEGVAITPGTDFGQYRANDFVRVAYTADIDRLAQAAARIARFVARLG"
     gene            8366..8809
                     /gene="dksA"
                     /locus_tag="PKI23_07870"
     CDS             8366..8809
                     /gene="dksA"
                     /locus_tag="PKI23_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNN36893.1"
                     /translation="MAAKEHSRAEAILKTFSPYKEKKGEEYMNPQQLAHFRNILQTWK
                     KELMEEVDHTVHHMQDEAANFPDPSDRASQEEEFSLELRTRDRERKLIKKIESTLERI
                     DQGEYGFCDACGIEIGIRRLEARPTASLCVDCKTLDEIKEKQQRG"
     gene            8823..9758
                     /gene="gluQRS"
                     /locus_tag="PKI23_07875"
     CDS             8823..9758
                     /gene="gluQRS"
                     /locus_tag="PKI23_07875"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNN36894.1"
                     /translation="MARLQAGTESPLHPPSRRYRGRFAPSPTGPLHLGSLLTAVASYL
                     DARQAGGDWLLRIENIDPVREASGAESQILHALEQHGLHWDGPLERQWSRMSHYREVA
                     ERLRERGLAYRCQCSRSELASLGGQHPATCRHRPPAANLPSALRVDVGDRPIRFIDLI
                     QGPQQFQLARDGGDFVIWRRDDLVSYQLAVLLDDLRQGISRVVRGVDLLDSTPRQIHL
                     AMLLGEPPPDYGHLPVLVNRSGAKLSKQNLAPALDLSRARQNLHAVLTLLRLEPPPAL
                     RHEAVRTQLDWATPRWSLAALQGCRSLPMPERLAD"
     gene            9812..9988
                     /locus_tag="PKI23_07880"
     CDS             9812..9988
                     /locus_tag="PKI23_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36895.1"
                     /translation="MYVPRSIMLLVIGVLLLFPGVQAWVGDVGSSWYRPHLVWLAVII
                     LALVLQQLREPDES"
     gene            9978..12935
                     /locus_tag="PKI23_07885"
     CDS             9978..12935
                     /locus_tag="PKI23_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN36896.1"
                     /translation="MSPELALPLAVLGYLALLFLTAWAAERWRGPARLARHPLIHLFA
                     LGVYGGAWSLLGSIGFAQHFGHSYLFYYLGMSMAFLLAPIFLQPLAQLVRTHQLNSLA
                     DVLAFRYRSRLVGALAALLLLFGSLPMVALQLKSVHELFVLLSHDGPAPWLLLGGFVL
                     LLLLFTLRFGSRDLTAEQQHSGLLLAIASSGLLKLVAFGGLGWLALRYAFGDLPGLGQ
                     WLRAHPLLLDRLHQPMAQWTPRILLLLFFFATVLMPHIYRLLFAEGRSATLSNWLGGG
                     FSLYLLLLSLPVLPILWAGESIGLSGPAEFFSIGLALTSHSPAILLATLLSTLAATSA
                     VMVVATLALASICLNHLVLPFYQTSKKEQLYRWVLARRRWLIALIFLIGLLGPMLFSD
                     WQPLAELALFAFVCILQLMPGTLGVLYWPHANRKGLVAGLLTGLVLWLLCALLAQPLT
                     GARSLSLLQDNWSPLALGCLAINIAIFSLVSAVTSRSPAERAAAELCSVDNIRRPRRR
                     ELSQSSPERFIQQLSKALGLNLARQEVEQALRELELDADERRPYAMRRLRDRIEANLS
                     GRLGPSTAQEIVSRLLPYKESPYAYDIEDINFIENRLESYRKDLRGLAAELDTLRRYH
                     RQTLRDLPIGVLSIGVDGEVLLWNRAMEALTQTPAGEVIGSTLASLAPPWGALFADFL
                     HDPAMQRHKEPIKLGGEQRWVALHKSDVSAEDVYQGGSQVILVEDMTDTRLLEQKLMH
                     SERLASIGRLAAGVAHEIGNPVTGIDCLAQNLAAESDEAEIRELAEQIRQQIRRINRI
                     VQSLVTFAHRGNPETPSRHGAVSLRACVAEAVELLSIGGKGYDIRFDNRCDPDLDVRG
                     DSQRLQQVFVNLLDNARDASPPGACILIRSQLLEGMVQTEVIDQGSGIDKSLHERVFD
                     PFFTTKEPGEGTGLGLALAYSIVEAHQGQLLIDSPLDAATGRGTKVTVLLPKWVESTQ
                     QSAQTDDSESETG"
     gene            12971..>13376
                     /locus_tag="PKI23_07890"
     CDS             12971..>13376
                     /locus_tag="PKI23_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN36897.1"
                     /translation="MKSTPKVLIVEDESVIRYALRRLLERNQFKVSEAGSVAEAQAKQ
                     NLDEFDIIISDLRLPGAPGTDLIKLAPATPVLIMTSYASLRSAVDAMKMGAVDYIAKP
                     FDHNEMLGTIRRIIASHTPPPVAAVGGSHGLPG"
BASE COUNT         2110 a         4459 c         4489 g         2318 t
ORIGIN      
        1 aacggcgccc tcccctcgac ccggcacagg ttcgccgaac aggtgtcgag gcaggcagcg
       61 cgctaccgcg agcaggggct gccgccgcgc gccgagcggt ttgcccgtac gctggccgac
      121 cactatcaac agccctggcc gcccgctccg gccagtttcg accccgatcg gtgctgaacg
      181 ccatgccgct gcgaatcgtc gccgacgaaa acatcccgct gctcgatccg ctgtttgcgc
      241 cgctcggcga agtggtcagg gtgcccggac gcgccatcac gccggcactg ctgcgcgacg
      301 ccgacctgct gctggtgcgc tcgctgaccc gcgtcgatgc cgagctggtg cgcggttcct
      361 cgatcaaatt cgtcggcagc gccaccatcg gtgtggacca cctggattgc gagccgctgc
      421 ggcagctcgg catcgaggtg gccaatgccc ccggctgcaa cgcccgcgcg gtggccgaat
      481 atctgctctc gctgctgctg acgctgcgcg aggcagagcg gttgccgccg caacccagag
      541 tggcggtggt ggggctgggc agtgtcggac agcagctctg cgcgctgctg cgcacccttg
      601 gttggccgct cgactgcgtc gacccctacc ggccggatct ggccaacagc gacctcgatg
      661 ccgccatgca ggccgatgtg atcacgctgc acaccccgct gacccggcag ggcgaccatc
      721 ccacctggca tctgttcgat gcggcgcgac tggcgcggct gcatgacggc cagttgctga
      781 tcaacagcgg ccgtggcgcg gtgatcgacc accaggcgct gctggcacgg ctgcaacagc
      841 ccagggcacc cagcgtggcg ctggatgtct gggagggtga gcccaacatc gacccggccc
      901 tgctcgatcg gctggtgctc ggcacgccgc acatcgccgg ccacagcctg gagggcaaga
      961 tgcgcggtgc ctggcagctg cgtcaggcga tcggtgcatg gctggagctg ccgccgctgc
     1021 cgccactcga caccttcctg ccgccaccgc tgctgcgcga ggtcacgctg gccgcggcgg
     1081 ccgatccgct cgacctgctg ctgcaggtga tgcgactgct ctacgacatc cgcctcgacg
     1141 actggcgatt gcggcgcgca ctggcgcagg acccttcagc cgccaccttc gaccggctgc
     1201 gcaagcacta ccccggccgc cgcgaatttg cctccctgcg gctgcggctg ccggccgagg
     1261 ccgaagccac ccgggcgctg ttcgccgcgc tgggttttgc ggtggtttga gaagagaagc
     1321 aaccccagcc gaggacggtt ggggttgtca ttgatcgatc aggtttttcg ccttggctgg
     1381 tcaccggaaa ggccgttcac cctaccccgg tcaccccttc gtgccgcagc cgccgccgct
     1441 tgcactccaa gacgcagcat ggcgccatgg ctggccacga ttttcgtgta ccctcggtca
     1501 tgagtgcgaa tgcaaagaag agaaccgatg ggcagagtga ttcgaatgtt gccattgctc
     1561 ggtgtactgg cgctggcatt ggcctgtgcg cgcagcgcac cggcggcggg caacgaaggg
     1621 cagccgattg caggcggcgc gttcgtgatc gatggcgaca ccctgacggt tgcggggcag
     1681 cgcattcgcc tgtacggcat cgatgcgcct gagtttcatc agctgtgtca acgcgatgga
     1741 gaaccctggc gctgcgggca agccgccacc gtggcgctga cggagttcat cgggcggcgt
     1801 ccggtgcgct gcgagcccag ggatcgggag ccggactcct tcgaacaggg gcgccaccgc
     1861 tacggcaggg tgattgcggt ctgttcggtg ggaggcgcgg agctcaaccg ctggctggtc
     1921 gccgagggac atgcgctcgc ttaccgccat tacagcagcg actacgtggc ggatgaaacg
     1981 caggcgcagc gcgcccgcaa gggggtgtgg ggctcggagt tcgagccgcc gtgggtgtgg
     2041 cgccaccagt cgaacggtgt cagccggcgg agcacgctgg cgccctccac cacgccaccc
     2101 gcgcgcactg ccgccgaagc ggagtgcggc accaagaagc gctgcgcgca gatgtcgagc
     2161 tgcgcggagg cgcgcgacca tctgacgcgc tgcgacatca agtcgctgga tggcgatggc
     2221 gatggggtgc cgtgcgagtc gctgtgccgg tagtctctgt tgtgccggcg cgactggcca
     2281 tttcggctta gaatggcgca attttgatct gctgttgcga gagccctgcc gatgccgaac
     2341 ctgcctgccg ccccgcccca cgccctgatc cgctggagtc accgtgtcga acccggattg
     2401 acgctgcgcg gctggcgcac gccgccctcc ggcaagccgc tgctgcactt cgtgcatggc
     2461 aacggcttct gcggcctcag ctattgggcg atgctgcgtc ggctcggcag ccactatgac
     2521 ctgtttctga gcgatgtgca ggggcatggc gacagcgatg ccggggagcg gttcgatggc
     2581 tggcaggtca ccgtggagcg gctctttgcg gtgatcgagc ggtttgcgcc cgagtggggc
     2641 gaggtgccgg tgcatggcgt tggccacagt tttggcggtg tgttgacgct gtcgctggcg
     2701 gcgctgcatc cgcagctctt cagcagcatc gtcgtgctcg acccggtgct gtttccgccg
     2761 gaattgatgc aggccgagat cgagagccgc cagcggggcg agtcgccgat ggcgcgtgac
     2821 gcgcggctgc gcaccgccct ctggccatcg cgcgccgctg cctggcgcca tctgcatcag
     2881 cgcggtggtt acaagggctg gagtgacgag gcgttcgcct gcttcatcga ccatgcgctg
     2941 tacgccaacg acgacggcca gtatcggctc aagtgcccgc cgcagatcga ggcggccatc
     3001 tacagctcgg tgccgctcga ttactggcgg ctgattccgc aggtcgactg cccgggcgtg
     3061 atactccatg gcgattcgac catgcctttc atcgcgccgg ccgtggcccg ggcgttgcag
     3121 ctcaatccgg ggttgcaggc cgagcaggtc acgggcggtc actgtttcat gcaggagcag
     3181 ccggccgcca ccgccgagcg ggtcgcgcag tggttgcagc ggctggcgcc gtcggcggtc
     3241 gactgtgccg ctacggcgcg gcaggcgctg cacactccgt agcggctgcg gtcgccggcc
     3301 gggcgctgat ctcggcacgg gtcgccacca tggtatcgcg cactgccggg gcgagcgcag
     3361 gctgtcgcag gccgcgttcg atcagtgtca acgcctggtc gcggcagccg agctgcgcca
     3421 gggtgtcggc caggttgttg agcgcgactg gatcctcggg acgcagccgc aacagtgcgc
     3481 ggtactgttg cgctgcggcg gcatggtcac cgcgggcgtg gagcatgttg ccggcaccca
     3541 gtcgggcggt gggttgatcc ggccagcgtt cgactgccgc cagatagcca cgcagcgcgg
     3601 ccgtgttgcg accctggctc tcccacacgg ccagttcggc cagataacgt gtgctgtcga
     3661 cgctggccag cagttcaccc ggcttcagca ccaccagtgc ccagtggtcg ctctgctgcc
     3721 aactgtagag aaactggcgc atcggcagcc gctggcgccg ttcggtgccg gagcgcagga
     3781 tcaccccctc gtgccgggca tcgtggccga tcaccaccgc atagtgccag cgcggccaga
     3841 aggagaaacc gagattctgc agcaccagca ccggccggcc ggcggccagt tcggcctcga
     3901 tcgccgacca gtgtggatcg agccggtagg gaatgcgacc ggcccggcgg ctggccgcga
     3961 tcagctccgg ttgcaggctg ccggcccgct gcggcaggta gacctgtggt gcaagctgat
     4021 cgggcgtggt ctcgaccccg gcatcgacca gcagcgtggc cagggccgcg ggtccgcact
     4081 ggtagcgtgc ctggggaaag aagggtgtcg cgctcagctc aagcgtcggc gaggcggctg
     4141 tcccggctgg cggcgaccac tgtgtgcagc cggccagcca cagcagcagc agggcgccca
     4201 gcgggcgcca gcgcagtgcg ctcaaacgga gcgaaagacg ttggtcgcgc cgacgaagtc
     4261 gagaatcagc agcacgataa agacgatacc gagcaccgcg aacaggccgt caccgccggc
     4321 tggcaactgg tcgatctgct gctggatcga ggcaatctcc gcgtcgctca ggttggcaat
     4381 gcgggcggcc gccgcctgcg ggtcgacacc cttctcgatc agttttgcct gcacatcggc
     4441 ccgtgccagc gcctgttgca gcggcgcgag ccggctctga cgctgctcca gcgtcagcgc
     4501 gctttcggtc gagatcagcg cggccgcggc cggctgcacg gtggccagcg ccaccgactg
     4561 ggtcaccagc agcgcgatca gtgaacgttt gagaagtctg gattgcatcg gtcagctcct
     4621 gaaagtcgta cggttgaagg cccatcttag cgcactccat tggccagggg atgcggtctg
     4681 gccgctcaat cggggtgacg atggccatgg tgggcgtcgg gaaagtgcgg atggtcgtgg
     4741 ctgagcgccg gatggtggtg aaagtggctg tggcgtgtgc cgggcggtac cggttcggca
     4801 tggctgtggt catggtgcgg atcgccatcc agtgcgtggt gatggcggtg gcggtgactc
     4861 atcggcgcat gatgatgcag atgctggtgt cgctccgtca gatgcagcgc cacacccacc
     4921 gccatcagca gtgcggccac cagcagttgc ggcgtgagcg gttcacccag cagcgcgatg
     4981 gccagcagcg caccccagaa cggcgccaga ccgaagtagg cgctggtgcg cgcggcgcca
     5041 agctgccgca gcgcgaccac gaacagcgtc aggctgacgc catagcagag gaggcccaac
     5101 agcgcggcag tcgccaccac gcccggcgct ggccagtcga cccgcagctg gagtgccagc
     5161 agcaggttgg tcgaaccggc gatcaatccc ttgctcatcg cgatgaaaga ggcatcggcc
     5221 agcgcgatct tgcgggtgag gttgttgtcg atcgcccagg ccagcgcggc agcgagcagg
     5281 gccagggtgg gccagagcgt ggccagttgc agccgctgcg gccagctcag cagcagggcg
     5341 ccggcgacga tggtcagcat ccccgctgca atgcggcggt cgaacggctc cctgaataga
     5401 aaccaggcca gcaacacggt cagcaccgcc tcggcattga gcagcagcgc ggccgtggtg
     5461 gcgggcatcg ccgacagcgc gaacatcagc agcagcggtg cggccacacc accggcggca
     5521 atggcggccg ccagccagcc ccagtcaccg cgttcgagcc gcactgcctc ggcacggccc
     5581 agccgccgca gcagccacag accgacgccg gagcccagat agagcagcgc ggccagcagc
     5641 caggggtcga tccgcgtcag cagcagcttg gccagcggtg tgctggcgcc gaacagcagc
     5701 gccgcggcca gcgcagcgag gatgccactg atgcgctggc cgttcatgat gcgtggcagg
     5761 cgcaggattc gccgtgggca gcggccacca gctcctgcag gatgccgcag tcacgggcgg
     5821 tgcgggcggt gccgcaccgc cggcgcagcg tatccagttg cagtgccagc gcctgcaggc
     5881 tgacgatgcg cgcctgcacc cggttcaact gcgccgcgat cagttgatcg atgtcaccgc
     5941 agtcggcctc gggctgctcg atgaagcgca gcagccgctg tacgtcggcc agcgggatgt
     6001 ccagcgcgcg gcagtggcgg atgaagctga gccgctccag gtgcgaacgg tcgtaggcgc
     6061 ggtagccgtt gtcatggcgc gccggcgccg gcagcagtcc gcttttttcg tagtagcgaa
     6121 tggtctcgac atcgacgcca gtggcccgcg ccagttcacc gatgcgcatg gcgtgtctcc
     6181 tctatcgaac cgggcttcat cgttgctgaa cgtgatgtta aatcgcttga ccctgtagtg
     6241 gctacggggt gttgaatcga tgactgtcct cattcatcag gagatcgcca tgtccgacaa
     6301 ctgctgcgga cccacccctg ctgtcgcgct caccccccgt taccgcaagg cgttgtgggt
     6361 cgccttgctg gtcaacagtc tgatgttctt cgtcgaggtc gccggcagcc tgcggtcagg
     6421 ctcctccgcg ctgctggccg atgcgatcga ttttgccggt gatgccgcca actacggttt
     6481 gtcactggca gtgctgtcgc tggcgctggt gtggcgctcg cgcgctgcct ggatcaaggg
     6541 cgccacgatg gcgctgttcg ggctgctggt gctgggcaga accggctggg cagcgctgca
     6601 cgggctgctg cccgagacct ggacgatggg tctggtggca ctgctggcgc tggccgccaa
     6661 tgccggcgtg gcgctgctgc tctatgcctt tcgcgatggc gatgccaaca tgcgctcggt
     6721 ctggctctgc agccgcaacg atgcgatcgg caatctggcg gtgattctgg ccgccgctgg
     6781 cgtggccctc accggcagcc gctggcccga cctgctggtg gcggtggcct tggcgctgct
     6841 ggcactgagt tcgggcgtct ccatcatgcg ccatgcccgc gatgagttga acagcgcgtt
     6901 gccatccgcc gtgcagagct gccaaagcga cccctgcggc cggtgaatgt cgccgttcat
     6961 cccagccgcg ccacgaagcg ggcgatgcgc gccgccgcct gcgccagtcg gtcgatgtcg
     7021 gcggtgtagg ccacccgcac gaagtcgttg gcgcggtact gaccgaagtc ggtgcctggg
     7081 gtgatggcca ccccttcttc ttcgagcagg cgcaggcaga aggtctcgct gtcgagtccc
     7141 agatggtcga tcgccgcata gaggtagaag gccccctccg gctcccgttc gatcacgagg
     7201 ccggcctgcc gcagcgccgg cagcagcaga tcgcgtcgcc gctggaactc gccacgctgc
     7261 gcttcgagaa gctcgatcgt cgatgccgaa aaggccgcca gcgccgcgtg ctgtgccggt
     7321 gtcgacgggg cgatgaacag gttctgcgcc aacttctcca gcggggcaac ggccgtgggc
     7381 ggcgccacca gccagccgag tcgccagccg gtcatgccga aatatttcga aaagctgttg
     7441 atcacatagg cttcgtcggt gaaggccagc atcgagggcg catcgctgcc gtaggtgagc
     7501 ccttggtaga tctcatccac cagcacaaag gcctcctttt gcacggccag ctcagcgaaa
     7561 ccacgcagat cgctccggtt cagcacggtg ccagtcgggt tggaaggggt tgccagcagc
     7621 aggccacggc tgttcggccg ccactgttgc tgcgccagcg ccggcagcag ttgaaagcgt
     7681 tgcgcggccg ccaccgccac cgcctgcggt tcgccgccga acagccggat gaagtggcgg
     7741 ttgcaggggt agcccggatc ggccagcagc acgccgctgc ccggctccac cagcagcgcg
     7801 gcggccagca gcagtgcgcc ggaggccccg gcggtgatga ggatgcgtcg cgggtcgatg
     7861 tcgagccctt gggtacgccg atagtggtcg ctgatcgccg cgcgcagttc gggcagcccc
     7921 agcgccgggc tgtattgagt ccggcccgca gccagcgcgg cccggccggc ttcgacgatc
     7981 ggtgcggcgg tgggaaaatc gggctcaccc acctccatgt ggatgatgtc ccggcccgcc
     8041 tgctccagcg tgcgggcctg ctccagcacc gccatcaccc gaaagggttc gatctcggcc
     8101 acccgggcgg cgaatcgccg gtcactcatg ccacatctcc tgctgtttca gcctgctgcc
     8161 gcggtcgatg gcgcaccgac agaaaagcaa cccgaggctc tggccaaagg gtaacctatc
     8221 tgatagcttc tgcgcgctgt tctgtcacgc ccgtccatgc tgccgacagc gtcgagggcg
     8281 tgcaggtcat tcaacccgcc ccatccatcc gatctggccg agtcggcgcc ggatcatcca
     8341 cgagccgtat cccggagcag aaaccatggc agccaaggag cattcacgcg ctgaagccat
     8401 tctcaagacc ttttcgccct acaaagagaa gaagggcgag gagtacatga atccgcagca
     8461 gctcgcacac ttcaggaaca tcctgcagac atggaagaaa gaactgatgg aagaggtcga
     8521 ccacaccgtc caccacatgc aggatgaggc cgccaacttc cccgatccct ccgaccgcgc
     8581 cagccaggaa gaggagttca gcctcgaact gcgcacccgc gaccgcgagc gcaagctgat
     8641 caagaagatc gaatcgaccc tcgagcgcat cgaccagggc gaatatggct tctgcgacgc
     8701 ctgcggcatc gagatcggca tccgtcggct cgaggcgcgc ccgaccgcca gcctctgtgt
     8761 cgactgcaag acgctggatg aaatcaagga aaagcagcaa cgcggctgac gccggcgggg
     8821 ccatggcgcg cctccaggct ggcaccgaat cccccctgca ccctccctcc cgccgttatc
     8881 ggggccgctt tgccccctcg ccgaccggac ccctccatct gggctccctg ctgaccgcgg
     8941 tggccagtta cctcgacgcc cgccaggccg gcggcgactg gctgctgcgg atcgagaaca
     9001 tcgatccggt ccgtgaggcc agcggggccg aatcgcagat tctgcatgcg ctggagcagc
     9061 atggtctcca ttgggatggc ccactggagc ggcaatggtc gcggatgtcg cactaccgcg
     9121 aggtggcgga gcggttgcgc gaacgcggtc tggcttaccg ctgccaatgc agccgcagcg
     9181 aactggcctc cctgggcgga cagcatcccg ccacctgccg tcatcgcccc ccggcggcca
     9241 atctccccag tgcattgcgg gtcgatgtcg gcgaccggcc gatccgcttc atcgacctga
     9301 ttcaggggcc gcagcaattt cagctcgccc gcgatggggg cgattttgtc atctggcggc
     9361 gcgatgacct ggtcagttat caactggcgg tgctgctcga tgacctgcgc caaggcatca
     9421 gccgggtggt acgcggggtc gacctgctcg actccacgcc gcgccagatc catctggcca
     9481 tgctgctggg agagccgcca cccgactatg gccatctgcc ggtgctggtc aatcgaagcg
     9541 gtgccaagct gagcaagcag aacctcgcac cggcgctcga tctctcccgc gcgcggcaga
     9601 atctccatgc cgtactgacg ctgctgcgac tcgaaccgcc gccggcactg cggcatgaag
     9661 cggtgcggac ccagctcgat tgggccacgc cccgctggtc gctggccgcg ctgcagggct
     9721 gccgcagcct gccgatgccg gaacggctgg cggattgagt ccggcctctc taccatagcc
     9781 ggtcgatccg tcatccattc aggacattgc gatgtatgtc ccgcgctcga tcatgctgct
     9841 ggtgattggg gtgctgctgc tctttcccgg cgtgcaggcc tgggtcggcg atgtcggcag
     9901 cagttggtat cggccccatc tggtctggct ggcggtgatc attctcgccc tggttctgca
     9961 gcagctgcgg gagcccgatg agtcctgagc tcgctctgcc gctggcggtt ctgggctatc
    10021 tggcgctgct gttcctcact gcctgggctg ccgaacgctg gcgcgggccg gcccgcctgg
    10081 cgcgccatcc gctcatccat ctcttcgcac tcggggttta cggcggtgcc tggtcgctgc
    10141 tggggtcgat cggcttcgcc cagcatttcg gccacagcta cctgttctac tatctcggca
    10201 tgagcatggc ctttctgctg gcgccgatct ttctgcagcc gctggcccag ttggtgcgta
    10261 cccatcagct caactcgctg gccgatgtgc tggcgttccg ttaccgcagc cgcctggtcg
    10321 gggcattggc cgcgctgctg ctgctgttcg gctcgttgcc gatggtggcg ttgcaactga
    10381 agagcgtgca tgaactcttc gtactgctga gccacgatgg ccctgccccc tggttgctgc
    10441 tgggtggatt cgtgctgctg ctgctgctct tcacgctgcg gttcggctca cgtgacctca
    10501 ctgcggagca gcagcattcg ggcctgctgc ttgcgatcgc cagctccgga ctgctgaagc
    10561 tggtggcgtt cggcgggctc ggctggctgg cgctgcgcta cgcctttggc gacttgccgg
    10621 ggctcggcca gtggctgcgg gcccatccgc tgctgctcga ccggctgcat cagccgatgg
    10681 cgcagtggac accacgcatc ctgctgctgc tgttcttctt tgccactgtg ctgatgccgc
    10741 acatctaccg cctgctgttt gccgagggtc gttcagcgac attgtccaac tggctgggtg
    10801 gtggcttctc gctctatctg ctgctgttga gcctgccggt gctgccgata ctgtgggccg
    10861 gcgaatcgat cggactgagc ggccccgccg aatttttctc gatcggtctg gcactgacga
    10921 gtcattcgcc ggcgattctg ctggcgaccc tgctcagcac cctggccgcc accagcgcgg
    10981 tgatggtggt ggcgacgctg gcgctggcct ccatctgcct gaaccatctg gtgctgccgt
    11041 tctaccagac ctccaaaaag gagcaactct atcgttgggt gctggcgcgc cggcgctggc
    11101 tgattgcgct gatctttctg atcggtctgc tgggtccgat gctcttttcc gactggcagc
    11161 cactggccga actggccctg tttgcctttg tctgcatcct ccagctgatg cccggtaccc
    11221 tgggggtgct ctactggccg catgccaacc gcaaggggct ggtggcggga ctgctgaccg
    11281 gtcttgtgct ctggctgctc tgtgccctgc tggcacagcc actgaccggt gcccgttcac
    11341 tgtcgctgct gcaggacaac tggagtccgc tggcactcgg ttgtctggcc atcaacatcg
    11401 ccatcttctc cctggtctcg gccgttacct cacgcagtcc ggcggagcgt gccgcagccg
    11461 aactctgctc ggtggacaac atccgccggc cgcgacgccg tgaattgagc caatcgtcac
    11521 ccgagcgctt catccagcag ttgagcaagg cgctcgggct caatctggca cggcaggagg
    11581 tggagcaggc gctgcgcgag ctcgagctcg atgccgatga gcggcgcccc tatgcgatgc
    11641 gccgtctgcg tgaccgcatc gaggccaacc tgtcgggccg gctgggaccc tccaccgccc
    11701 aggagatcgt ctcacgcctg ctcccctaca aggagtcacc ctatgcttac gacatcgagg
    11761 acatcaactt catcgaaaac cggctcgaat cctatcgcaa ggatctgcgc ggcctggcgg
    11821 ccgaactcga caccctgcgg cgttaccacc gccagacatt gcgcgacctg ccgatcggag
    11881 tgctctcgat cggtgtcgat ggcgaagtgc tgctgtggaa ccgcgcgatg gaggcgctga
    11941 cccagacgcc agccggagag gtgatcggct ccaccctggc ctcgctcgca ccaccctggg
    12001 gagcgctgtt cgcggatttt ctacatgacc cggccatgca gcgccacaag gagccaatca
    12061 agctgggcgg ggagcagcgc tgggtggccc tgcacaagtc ggatgtctcc gccgaggatg
    12121 tctatcaggg tggcagtcag gtgatcctgg tggaggacat gaccgacacc cgactgctcg
    12181 aacagaagct gatgcacagc gagcgactgg cctcgatcgg ccggcttgcc gccggtgtcg
    12241 cccatgagat cggcaatccg gtcaccggca tcgactgtct ggcgcaaaac ctcgctgccg
    12301 agagtgacga agccgaaatc agggagctgg ccgagcagat tcgccaacag atccgccgca
    12361 tcaaccgcat cgtccagtcg ctggtcacct ttgcccaccg cggcaacccc gagacaccct
    12421 cccggcatgg tgccgtctcg ctcagggcct gtgtggccga agcagtcgaa ctgttgtcga
    12481 tcggtggcaa ggggtatgac atccgcttcg acaaccgctg tgatccggac ctcgacgtgc
    12541 gtggcgacag ccagcggctg cagcaggtgt tcgtcaacct gctcgacaac gcccgtgacg
    12601 cctcgccacc cggtgcctgc atcctcatcc gcagccagct tctggagggc atggtccaga
    12661 ccgaggtgat cgatcagggc agcggcatcg acaagagcct gcacgagcgg gtgttcgatc
    12721 cgttcttcac caccaaggag ccgggcgagg gcaccggact cggcttggca ctggcctaca
    12781 gcatcgtcga ggcgcatcag gggcaactgc tgatcgacag cccgctggat gcggcgaccg
    12841 gacgcggcac caaggtaaca gtgctgctgc caaagtgggt ggaatcaacg caacagagcg
    12901 cgcagacgga tgattctgag tcagaaactg gttagactgg taacgtctta tactgccgag
    12961 gtcgtgagta atgaagtcga cccccaaggt gctcatcgtc gaggatgagt cggtcatccg
    13021 ctacgcactg cgccgtctgc tggagcgcaa ccagttcaag gtcagcgagg ccggttcggt
    13081 ggccgaggcc caggcgaagc agaacctgga cgagttcgac atcatcatca gcgacctgcg
    13141 cctgcccggt gcgccgggca ctgatctgat caagctggcg ccggcgacac cggtgctgat
    13201 catgaccagt tacgccagcc tgcgctcggc ggtcgatgcc atgaagatgg gcgcggttga
    13261 ctacatcgcc aagcccttcg accacaacga gatgctgggc acgatccgcc gcatcatcgc
    13321 cagccacacg ccgccaccgg ttgccgccgt cggcggcagc cacggcctgc ccggca
//
LOCUS       DAORCQ010000065        13236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3026_length_13236_cov_5.136105, whole genome shotgun sequence.
ACCESSION   DAORCQ010000065 DAORCQ010000000
VERSION     DAORCQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_13236_cov_5.136105"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1765)
                     /locus_tag="PKI23_07895"
     CDS             complement(110..1765)
                     /locus_tag="PKI23_07895"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNN36898.1"
                     /translation="MTTTLRVVMAQINPLVGDIKGNVELVMQQVRRAVAELRAEVVLF
                     PELVITGYPPEDLLLRPSMQIRVEDALQQLRQFSVEEPLCRGVDIVVGYPFQKKSGRY
                     NMAGVIRDGELIAEYRKQRLPNYQVFDEKRYFQPGGAPCVYDRDGLRIGLTICEDVWQ
                     EGPVQQAADAGAQLLLNLNASPFHRRKPSERRELLRHRAIEGRLPIVYVNQVGGQDEL
                     VFDGASMVVDRHGDCVVQMESFTEGLCLVEFSGDGNVCDVISRQPLADLTLEASIYNA
                     LVVGVRDYVDRNGFSGVVIGLSGGIDSALTLAIAVDAVGADRVQAVMMPFHYTSAMSL
                     EDAEAQARVMGVDYSIISIAPMYDAFMAGLETEFAGLAPDTTEENLQARCRGVLLMAI
                     SNKKRSMVLSTGNKSEMAVGYSTLYGDLVGGFAVLKDVPKTVVYALCHYRNGIAPVIP
                     QRVIDRPPSAELRPDQKDQDSLPPYPLLDAIIELYIENDLSADAIVARGFDEAVVHRV
                     VRMIDRNEYKRRQAPIGVRISQRAFGRDRRYPITSGWSLKDRF"
     gene            1910..2137
                     /locus_tag="PKI23_07900"
     CDS             1910..2137
                     /locus_tag="PKI23_07900"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNN36899.1"
                     /translation="MLIRLFFVALLLFACYLYLRHRRSRRAAERPLGQFVRCAQCELH
                     LPAEQAIRDGDHWFCGEPHRARYRAINRPKP"
     gene            2140..>2840
                     /locus_tag="PKI23_07905"
     CDS             2140..>2840
                     /locus_tag="PKI23_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNN36900.1"
                     /translation="MATLFATPQQSLPRTDRQVVRVYLYYRLILAIVLLVMRFGDEGQ
                     IHLGQLLPRLYLSVALCYLLSALLAPLLHALLFGSSLNTARLFALFSFDILLFTLLMY
                     SSSGVTSGLGNLSLIAVAAGNILLQGRIGTLLAALATLLVIGEEGYLLLTALPPGEES
                     NRFFQAGVLGTLYFAIALGMQMLSRRLLASDELARQRARDISELQQLNHMIIQRMRTG
                     ILVLDRNDDIRMINRA"
     assembly_gap    2841..2940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2941..3971
                     /locus_tag="PKI23_07910"
     CDS             <2941..3971
                     /locus_tag="PKI23_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNN36901.1"
                     /translation="TGILVLDRNDDIRMINRAATLLTQIPPTAQGGLRQLSEPLFSRL
                     EQWRRQPRLRPPPFSLPRGPQLQVSFARLTDQPDSDTLLFIEDQALLAQQAQQLKLAS
                     LGRLTASIAHELRNPLSAVNHAAQLLDELALPDAEQRRLTHIIQSNARRMNGIIENVQ
                     QLSRRDHARAGLLPLAPWLERFLHESILPAWPEAELVWRIEPESIEVRVDPGQLAQIL
                     TNLIENGLRYSRLATGRASLRIEAGIDGDHDLPYLAIIDQGEGVADEALDSLFEPFFT
                     TAPKGSGLGLYIARELCQSNQATIDYQRDAGGGSRFVITFSHPERSPGHQDGKEPTDA
                     EEADPDRR"
     gene            3922..5319
                     /locus_tag="PKI23_07915"
     CDS             3922..5319
                     /locus_tag="PKI23_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNN36902.1"
                     /translation="MAKSPQTQKKQILIVDDEPDLRELVALTLSRMGHGCHAAEDLRS
                     ARQLLQTQRFDLCLTDMRLPDGNGLELVEHIQLHHPALPVAMITAHGSMDSAIAALKA
                     GAFDFVTKPVELAQLRELVEKALAPMQPLAARVAALPELARLSGDSPVMQALRSQIRK
                     LARSQAPVHIQGESGTGKELVARLIHQLGPRGDGPFVPVNCGAIPSELMESEFFGHRK
                     GSFSGAVEDKPGLFQAASGGTLFLDEVADLPLPMQVKLLRAIQEKRVRAIGAQQEQPV
                     DTRILSATHKNLRALIEQGLFRDDLYYRINVIELRVPSLRERVEDIPQLAHELLARIA
                     LEYQLPPPELTSAAIERLCRHGFPGNVRELENILERAFALCEGERIEAADLLLDGESA
                     TATTTEPAERAVRPATPEAAMPAGSLEHYLEQIERQAIIGALEETRWNKTQAAKKLGI
                     TFRALRYRLKKLNLE"
     gene            complement(5306..6409)
                     /gene="thiO"
                     /locus_tag="PKI23_07920"
     CDS             complement(5306..6409)
                     /gene="thiO"
                     /locus_tag="PKI23_07920"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014716864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNN36903.1"
                     /translation="MHDFLIIGGGVIGLTCALRLAEAGASVTLLERGEFGKESSWAGG
                     GILAPLYPWRYAETINALALRGRQLYDQWLPRLHRQSGIDPELHRCGLLLLDSDCIAE
                     ASDWARQWRHSWVRLDGAALTTHFPTLSPRTGEALWLPEQASLRNPRLLQALLAVLAD
                     MPRVRLVPQCTVSGLRTVGGRVEAVLGSQGEWCADQVIVTAGAWSSSLLPTPPTLPVT
                     PVRGQMLLYQGNGLLSTMLLAAGHYLIPRRDGLILVGSTVEQVGFDCAVTAAARTELM
                     AVAGQLLPALAEREPVAHWAGLRPGAPQGTPFIGRWPGIDNLHLNCGHFRNGLVMAPA
                     AAELLVDLLLGQPPRVDAAAFDPARLLSAAIRD"
     gene            complement(6480..6920)
                     /locus_tag="PKI23_07925"
     CDS             complement(6480..6920)
                     /locus_tag="PKI23_07925"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNN36904.1"
                     /translation="MMHDRANGSRQRPGGFSLIELMVALAIVGILAAVAYPAYRDSIM
                     KSRRADAKIALTQGAAQQEKFFSRFSHYSGTLSDIGGATSPEGYYGLALATTNAGLGF
                     TLTATPTTRSQQHKDLRCATYTLSHTGAKVAKNSNNTTSTDLCW"
     gene            complement(7009..11808)
                     /locus_tag="PKI23_07930"
     CDS             complement(7009..11808)
                     /locus_tag="PKI23_07930"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNN36905.1"
                     /translation="MPAALSRLLTLLTLLLSAPLFAAPTGNPSNYTSLPVTAVEATPP
                     LLLIAMSRDHELFRKAYSDYSDIDGDGQIDTTYKDSIEYLGYFDSNFCYQYDNGKFSP
                     IGQVNSGTHKCSTVDGWSGNFLNWASMTRIDLIRTVLYGGKRRVDPNTSSGAVLLERE
                     LIPPDIHAFAKVYDGAASGAIALADLVEGSPTNGAITLCNVSQWPASTNLSGTLNTTS
                     YPPLLRVAYGNVAAGKVGYPNWASTEVAQCQWHGTTHRDSALAQPDRPVGNDTSNAYQ
                     VLVERCVAGQDGPLSARCKRYDNGTPNNASDDLFKPIGLLQRYGERGQMRFGLMTGSY
                     DNNSHGGVLRQGVGLLSEVNVATGQITAGSLIGTLDKLRIATYQMDFSTSSLLRYPPC
                     NSYVSSAAVKDGSCADWGNPLGELYLEAVRYLAGASAASAAFTSSPSPSRYSPAPTEA
                     AMIPGLTRLTSWSDPYNFGPGNSAVPCAQCAVLLISTSQGSFDSMRDAADWPSSGLPT
                     GFAVATATNAVQNKEGLDGASVLAGRVGSSTDQECSPKTLAAGTLDQADGICPEAPSA
                     EGGYGIAGLAYEAAIKDLRTGLNGTQKIKTFAVSVAEDLPSFTVTSGTKQAQIVPLCR
                     SEYSANGNAPYLLQKCSLIDVVSEPPPAPATLPSPCTNNGSYHRFLLTWEGTAWGSDY
                     DFDAEQRLEYCEVNGTLHVRTVVTRDQADAAFDMGFTLVGTDPQQPMNYPKEITVAQG
                     SNGGGTASGFQNLSITGGGTTLQKPLWLAAKYGGFNDLDSSNSPNDPREWDSVNNLTG
                     AAGSDGVPDNYFQLRSPSSLEAALNRIFASVAGSVASGTAAAVVANNVSGEGAVYQAL
                     YHPRIEAGQNSITWAGTLHGLFIDGQGRLREDFGGNSPTTGNATLDGCDVDPVVEIFF
                     DSVNLPTGTKIRRYSGVTADCSNIKIVTPTVRPLTDLKTLWNARDELARAANHAPLMP
                     QRNYADIASNGRAIYTRDPAATDGTLSDFTSTALASRFRELNPDTSSANPQGEATDII
                     NFIRGQEIAGYRSRTIDYDGNGAKPWLLGDIINSSPVVVGAPNAGHDLRLGDSSYAAF
                     KAKYAKRRQVVYVGANDGMVHAFNGGFWDEANKQFATTANNGETSHPLGAELWAYVPQ
                     NLLPHLRWLTAPNYEHIFYVDGTAQSFDVKAFANDSDHPDGWGTILVIGMRFGGVPID
                     IDDDGDGNTPDRSYRSAYLVLDITNPEQPPKLVAELSDGQLGFTTALPALVRRPNNGW
                     ELLFGSGPTASTPLASADAVSSQNAQLYRYDLVNKAFVTGFAPLDLGSTAVASFVGDP
                     LAQDWDGDGNTDEIYFGLVGGTAGSQSGGLYRADPDLGSVTQVIGTRPIIAAPMAVRD
                     NAGKKWLYAGEGKLLAASDRNETGQNSFYGVLLDSSNTALLRTDQIQVANTGLLIDSS
                     GTATPLVDGTVTINDFNTLKNHISTNKKGWVYQLAAPAPDPSARVINPATRAGSTLFF
                     TDFLPPTGSSCDFGQSRLHGLDYQSGTAAPQAAFGDTQTPNNALRLTLPYLSLGSGLS
                     SKPVVYSGEGGKRSIITQQETGALTGSEFTGSTVSPGRKSWRRLPLR"
     gene            complement(11822..12481)
                     /locus_tag="PKI23_07935"
     CDS             complement(11822..12481)
                     /locus_tag="PKI23_07935"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNN36906.1"
                     /translation="MADRLQSTFGTPARQRGIILLVSLILLLILTVMGLASMGGSLLE
                     ARMTGHYRDQELAIQSAEAALREGERTIHSSSSALLDARIKAGGNSCTGGYCQSRQFD
                     TGHAASLGKACHQAGHLYERWELPVCDSGTPVGSVDVWNNAGRHLTYGANLPGVRAPA
                     RYIIELAGLARPATAPPEDCAGHPFDPANAGCQRVYRITALAGGGTDRAQVMLQSTYV
                     K"
     gene            complement(12474..>13236)
                     /locus_tag="PKI23_07940"
     CDS             complement(12474..>13236)
                     /locus_tag="PKI23_07940"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNN36907.1"
                     /translation="VVQGSDLLVVKRADQRIDALVGGVIAPDHPKVALHPASEWDLNP
                     DNEAKTFATSTAAQKKSRRNLFLLLSSCVSADLFQNCDDGASITNRDLDRTSPCNNAT
                     GTALTPGNRAAVWSTPITQYSELYGVKNTVFYIGRNPAGQPALYRKVYRDETGEDAEE
                     LAEGVESLQLRYGIDSDGDGVANSQVSADAVGSNPVVTIEIALLLVSADDIKSQPAAE
                     TFDLAGTQFTSLADRKLRKVVSTVIKLRNRGRFDG"
BASE COUNT         2086 a         4217 c         4490 g         2343 t
ORIGIN      
        1 cgtcggcttt ttcaaacggc tgatcggcag cggcgatgcg cctgcggccc agccctctgc
       61 cgcacccccg ccgccgacgc gccacccgac cggtggcgtg cgctagcctt cagaaccgat
      121 ccttcagact ccagcccgag gtgatcgggt agcgccggtc gcgaccgaag gcgcgctggc
      181 tgatgcgcac gccgatcggc gcctggcgcc gcttgtattc gttgcggtcg atcatccgca
      241 ccaccctgtg caccaccgct tcgtcgaaac cccgggcgac gatcgcgtcg gcgctcaggt
      301 cattctcgat gtagagttcg atgatcgcgt cgagcagcgg gtaggggggc agggagtcct
      361 ggtccttctg gtcgggacgc agctcggcag agggtggccg gtcgatcacc cgctgcggga
      421 tgaccggcgc gatgccattg cggtagtggc agagcgcgta gaccaccgtt ttcggcacat
      481 ccttcagcac cgcaaagcca ccgaccaggt cgccatagag tgtggagtag ccgaccgcca
      541 tctcgctctt gttgccggtg ctgagcacca tgctgcgctt tttattggag atggccatca
      601 gcagcacccc gcggcagcgc gcctgcaggt tctcttcggt ggtgtcgggg gcaaggccgg
      661 cgaactcggt ttccagcccg gccatgaagg cgtcgtacat cggtgcgatc gaaatgatgc
      721 tgtagtcgac ccccatcacg cgggcctggg cttcggcatc ctccagactc atggccgagg
      781 tgtagtgaaa cggcatcatc actgcctgca cccggtcggc gccgactgcg tccacggcaa
      841 tggccagcgt cagtgccgaa tcgatgccgc ccgacagccc gatcaccacg ccgctgaaac
      901 cgttgcggtc gacgtagtcg cgcacgccca ccaccagcgc gttgtagatc gaggcttcga
      961 gcgtgaggtc ggccagtggc tggcggctga tgacgtcgca aacgttgcca tcgccgctga
     1021 actcgaccag gcagagccct tcggtgaagc tctccatctg caccacgcag tcgccatggc
     1081 ggtcgaccac catcgacgcg ccatcgaaca ccagctcatc ctggccgccg acctggttca
     1141 catagacgat cggcaggcgg ccctcgatgg cgcggtgcct gagcagttcg cgccgttccg
     1201 agggtttgcg gcggtggaag ggcgaagcat tgaggttgag cagcagttgg gcgccggcat
     1261 cggcggcctg ctggaccgga ccctcctgcc agacatcctc gcaaatggtc agcccgatgc
     1321 gcaggccgtc gcggtcgtag acgcagggcg ccccgcccgg ctgaaaatag cgcttttcat
     1381 cgaagacctg gtagttcggc agccgctgct tgcggtactc ggcgatcagt tcgccatcgc
     1441 ggatcacccc ggccatgttg tagcggccgc tcttcttctg aaaggggtag ccgacgacga
     1501 tgtcgacgcc gcgacagagt ggctcttcga cgctgaactg ccgcagctgc tgcaatgcgt
     1561 cctccacccg gatttgcatg ctgggccgca gcagcaggtc ctcgggcgga tagccggtga
     1621 tcaccagctc gggaaagagc accacctcgg cgcgcaactc ggcaacggcc cggcgcacct
     1681 gctgcatcac cagttcgaca ttgcccttga tgtccccgac cagggggttg atctgcgcca
     1741 tcaccacacg caaagtggtt gtcatagtgc ctcgttcgtt ctggtgtcga ttggatgacg
     1801 gcggtattgt aatgactgtt gtcagaaggc caaaaccgtc cgtgccggtc gatctttcga
     1861 ccgtgactgc cggtctgccg cacgcccgtc cgtcgctttc gtggtcacca tgctgatcag
     1921 actctttttt gtcgcgctgc tgctgtttgc ctgctatctg tacctgcgcc atcgacgcag
     1981 tcgccgtgcg gcggagcgtc cgcttgggca gttcgtccgc tgcgcccagt gtgaactgca
     2041 tctgccggcc gagcaggcga ttcgcgatgg cgaccactgg ttctgtggtg aaccgcaccg
     2101 cgcgcgttac cgtgccatca accgccccaa gccgtgaacg tggccacact cttcgcgaca
     2161 ccgcagcagt cgttgccacg caccgaccga caggtggtcc gtgtctacct ctactatcgg
     2221 ctgatactgg cgatcgtcct gctggtgatg cgctttggcg acgaggggca gatccatctc
     2281 gggcagttgc tgcccagact ctacctgagc gttgcgctct gttacctgct cagcgcgctg
     2341 ctggcgccac tgctgcatgc gttgctgttc ggctccagcc tcaacaccgc ccggctgttc
     2401 gccctgttca gcttcgacat cctgctgttc acgctgctga tgtacagcag cagcggggtg
     2461 accagcgggc ttggcaacct cagcctgatc gcggtggcag ccggcaacat cctgctgcaa
     2521 ggacgaatcg gcaccctgct ggcggcgctg gccacgctgc tggtgatcgg tgaggagggc
     2581 tatctgctgc tgaccgcgct gccgccgggc gaggagtcca accgtttctt tcaggccggt
     2641 gtgctgggca cgctctactt tgccattgcg ctcggcatgc agatgttgtc gcggcggctg
     2701 ttggccagcg atgaactggc acggcaacgg gcacgtgaca tcagcgagtt gcagcagctc
     2761 aaccacatga tcatccagcg gatgcgcacc ggcatcctgg tgctcgaccg caatgatgac
     2821 atccggatga tcaaccgcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 gcaccggcat cctggtgctc gaccgcaatg atgacatccg gatgatcaac cgcgccgcga
     3001 ccctgctgac gcagattccg ccgacggcac agggtggatt gcggcagctc tccgaaccgc
     3061 tgttcagccg cctcgaacag tggcggcgcc agccccggct gcggccgcca ccgttctcgc
     3121 tgccgcgagg tccccagttg caggtgagct tcgcccgcct caccgaccag cccgacagcg
     3181 acaccctgct cttcatcgaa gaccaggcac tgctggcgca gcaggcgcag cagctcaaac
     3241 tggcgtcgct gggccggctc accgccagca tcgcccatga gttgcgcaac ccgctgagtg
     3301 cggtcaacca tgccgcccag ttgctcgacg agctggcact gcccgatgcc gagcagcggc
     3361 gattgaccca catcattcag agcaatgccc gcaggatgaa tggcatcatc gagaatgtgc
     3421 agcagctgtc acgccgcgac catgcccgcg ccgggctgct gccactcgcg ccctggctcg
     3481 agcggttcct gcacgagagc attctgccgg cctggcccga ggccgagctg gtgtggcgga
     3541 tcgagccgga atcgatcgag gtgcgggtcg atcccggcca gttggcccag attctgacca
     3601 acctgatcga aaatggcctg cgttacagtc ggctggcgac cggccgggcc agcctgcgca
     3661 tcgaggccgg catcgacggc gatcatgatc tgccgtatct tgccatcatc gatcagggag
     3721 agggggttgc cgacgaggcg ctcgactcac tgttcgaacc gttcttcacc acggcgccca
     3781 agggctccgg cctcgggctc tacattgccc gtgagctgtg ccagagcaac caggcgacca
     3841 tcgattatca gcgtgatgcc ggcggcggca gccgcttcgt catcaccttc agccacccgg
     3901 agcgcagccc aggtcatcag gatggcaaag agcccacaga cgcagaagaa gcagatcctg
     3961 atcgtcgatg acgagcccga cctgcgcgag ctggttgcac tgacgctgtc gcggatgggg
     4021 cacggctgcc atgccgcaga agatctgcgc agcgcacgcc aactgttgca gacgcagcgt
     4081 ttcgacctct gtctgaccga catgcggctg cccgatggca acgggctcga actggtcgag
     4141 cacatccagt tgcaccatcc ggcgctgccg gtcgcgatga tcaccgccca tggcagcatg
     4201 gacagcgcca ttgccgcgtt gaaggccggc gccttcgact tcgtcaccaa accggtggag
     4261 ctggcgcaac tgcgcgaact ggtcgagaag gcgctcgcgc cgatgcagcc gctggccgcg
     4321 cgcgtcgccg cactgccgga gctggcgcgc ctctccggcg actccccggt gatgcaggcg
     4381 ctgcgcagcc agattcgcaa gctggcgcgc agccaggcgc cggtgcacat ccagggcgag
     4441 tccggcaccg gcaaggagct ggtggcacgg ctgatccatc agctcgggcc gcgcggcgac
     4501 ggccccttcg tgccggtcaa ctgcggtgcc atcccttcag aattgatgga gagcgagttc
     4561 ttcggccacc gcaagggcag cttcagcggc gcggtggaag acaagccggg gctgttccag
     4621 gcggccagcg gcgggacgct gtttctcgac gaggtggccg acctgccgct gccgatgcag
     4681 gtcaagctgc tgcgggcaat ccaggagaaa cgggtccgcg cgatcggtgc ccagcaggag
     4741 cagccggtcg acacccgcat tctcagtgcc acccacaaga acctgcgcgc gctgatcgaa
     4801 caggggctgt ttcgcgatga cctctactac cgcatcaacg tcatcgagct gcgggtgccg
     4861 agcctgcgcg agcgggtcga ggacattccg caactggccc atgagctgct ggcccgtatt
     4921 gcgctggagt atcagctgcc gccgccggag ctgacctccg ccgccatcga gcggctctgt
     4981 cgccatggct tccccggcaa tgtgcgcgag ctggagaaca tcctggagcg cgccttcgcg
     5041 ctctgcgaag gcgagcggat cgaagccgcc gacctgctgc tcgatggcga gagcgccacg
     5101 gccaccacca ctgaaccggc cgagcgcgca gtgcgccccg ccacgcccga agcggccatg
     5161 ccggccggct cgctggagca ctatctggag cagatcgagc ggcaggcgat catcggtgcg
     5221 ctcgaagaga cgcgctggaa caagacccag gcggccaaaa aactcggcat cacctttcgc
     5281 gcgctgcgtt accggctgaa gaagctcaat ctcgaatagc cgccgacagc agccgcgccg
     5341 gatcgaaggc tgcggcatcg acccggggcg gctgccccag cagcagatcg accagcagct
     5401 cggccgccgc cggcgccatc accagcccgt tgcgaaagtg gccgcagttg aggtgcaggt
     5461 tgtcgatgcc cggccagcgg ccgatgaaag gtgttccctg cggtgcgccg ggccgcagac
     5521 cggcccagtg ggcgaccggt tcgcgctccg ccagcgccgg cagcagttgg ccggccaccg
     5581 ccatcagctc ggtgcgcgcc gcggcggtca ctgcgcagtc gaagccgacc tgctccacgg
     5641 tgctgccgac cagaatcaag ccatcacggc gcggaatcag atagtggccg gcggccagca
     5701 gcatggtcga cagcaagcca ttgccctgat agagcagcat ctgcccccgc accggcgtca
     5761 ccggcagcgt gggcggcgtt ggcagcagtg agctgctcca ggcgccggcg gtgacgatca
     5821 cctgatcggc gcaccactcg ccctgactgc cgagcaccgc ctcgacccgg ccaccgacgg
     5881 tgcgcaatcc gctcacggtg cactgcggca ccaggcgcac ccggggcatg tcggccagca
     5941 ccgccagcag cgcctgcagc aggcgcgggt tgcgcaggct ggcctgctcc ggcagccaga
     6001 gcgcctcccc ggtccggggc gacagtgtcg gaaagtgcgt tgtgagcgcc gcgccgtcga
     6061 gccgcaccca ggagtggcgc cactgccgcg cccagtcgct ggcctcggcg atgcagtcac
     6121 tgtcgagcag cagcaggccg cagcggtgca gctccgggtc gatgcccgac tgccgatgca
     6181 gccgcggcag ccactggtca tacagctggc ggccgcgcag cgccagggcg ttgatggtct
     6241 ctgcatagcg ccacgggtag agcggcgcca gaatgccgcc gccggcccag gaggactcct
     6301 tgccgaactc gccccgctcc agcagcgtga cgctggcacc ggcctcggcc agccgcagtg
     6361 cgcaggtcag gccgatgacg ccgccgccga tgatcagaaa gtcgtgcatc gaacggaatc
     6421 gatggccggg cggctgctgc tggcagaggt gaccatcatg ctgccggacg gctcgcgcgt
     6481 caccagcaga ggtcggtgga ggtggtgttg ttgctgttct tcgccacctt ggcgccggtg
     6541 tgggagagcg tgtaggtggc gcacctgagg tccttgtgtt gctggctcct ggtcgtcggc
     6601 gtggcggtca gcgtgaagcc gaggccggca ttggtggtgg cgagcgccag cccgtagtag
     6661 ccctcgggcg aggtggcgcc accgatgtcc gacagtgtgc cgctgtagtg gctgaagcgg
     6721 ctgaagaact tctcctgctg cgcggcccct tgcgtcagtg cgatcttggc atcggcgcgg
     6781 cggctcttca tgatgctgtc gcggtaggcc gggtaggcca cggccgccag gatgccgacg
     6841 atggcgagcg ccaccatcag ctcgatcagg ctgaagccgc ctggacgctg gcgactgcca
     6901 ttcgctcgat catgcatcat gcttccactc cggtcagaca gattggccgc ggttatacca
     6961 tgaaaacgcg gccaatttgt ccgactgcgt cactgtatcg gccgctgctc agcgcagcgg
     7021 cagccggcgc cacgatttgc gccccggcga gaccgtgctg ccggtgaact cgctgccagt
     7081 cagcgcgccg gtctcctgct gggtgatgat cgagcgcttg ccgccctcgc cgctgtagac
     7141 caccggtttg gaggagagcc cgctgcccag gctcaggtag ggcagtgtca gccgcagtgc
     7201 gttgttggga gtctgggtgt caccgaaggc cgcctggggt gcggcggtgc cgctctggta
     7261 gtccagccca tggagcctgc tctgcccgaa gtcgcaactg ctgccggtcg gcggcagaaa
     7321 gtcggtgaaa aagagcgtgc tgccggcgcg ggttgccgga ttgatcaccc gtgcgctggg
     7381 gtcgggcgcg ggcgctgcca actggtagac ccagcccttc ttgttggtgc tgatgtggtt
     7441 cttcaacgtg ttgaagtcgt tgatggtgac tgtgccatcc accagcggcg tcgcggtgcc
     7501 actgctgtcg atcagcaggc cggtgttggc gacctgaatc tgatcggtgc gcagcagcgc
     7561 cgtgttgctg ctgtcgagca gcacgccata gaagctgttc tggcccgtct cgttgcggtc
     7621 ggaggcggcc agcagcttgc cttcgccggc gtagagccat ttcttgccag cgttgtcgcg
     7681 caccgccatc ggtgcggcga tgatcgggcg ggtgccgatc acctgggtga cgctgccgag
     7741 gtccggatcg gcccggtaga gcccgccgct ctggctgccg gccgtgccgc cgaccaggcc
     7801 gaagtagatc tcatcggtgt tgccatcgcc atcccaatcc tgcgccagcg ggtcgccgac
     7861 gaagctggcg accgccgtac tgccgaggtc gagcggtgca aaaccggtta cgaacgcttt
     7921 gttgaccagg tcgtagcggt agagctgtgc attctggctg ctcaccgcgt cggcgctggc
     7981 cagcggcgtc gaggcggtcg ggccggagcc gaacagcagc tcccagccgt tgttggggcg
     8041 ccgcaccagc gccggcagcg cggtggtgaa gccgagctgg ccatcgctca gttcggccac
     8101 cagtttgggc ggctgctccg ggttggtgat gtcgagcacc agataggccg agcggtagct
     8161 gcggtcgggg gtattgccat caccatcgtc gtcgatgtcg atcggcaccc cgccaaagcg
     8221 catgccgatc accagaatgg tgccccagcc gtcggggtgg tcactgtcgt tggcaaacgc
     8281 cttgacgtcg aagctctggg cggtgccatc gacatagaag atgtgctcat agttgggcgc
     8341 ggtcagccag cgcaggtgcg gcagcaggtt ctgcggcaca taggcccaga gttcggcgcc
     8401 gagcgggtgg ctggtctcac cgttgttggc ggtcgtcgcg aactgcttgt tggcctcgtc
     8461 ccagaagccg ccattgaagg cgtgcaccat gccgtcgttg gcgccgacat agaccacctg
     8521 ccggcgcttg gcatatttgg ccttgaaggc ggcatagctg ctgtcgccaa ggcgcaggtc
     8581 atggccggcg ttgggcgcgc ccaccaccac cggcgagctg ttgatgatgt cgcccagcag
     8641 ccagggtttg gcgccattgc catcgtaatc gatggtgcgg ctgcggtagc cggcgatctc
     8701 ttggccacgg atgaagttga tgatgtcggt ggcctcgcct tgcggattgg ccgagctggt
     8761 gtcggggttc agctcgcgaa agcggctggc cagggcggtg ctggtgaagt cgctcagggt
     8821 gccatcggtg gcggcagggt cgcgggtgta gatcgcccgg ccgttgctgg cgatgtcggc
     8881 atagttgcgc tgtggcatga gtggggcatg gtttgccgcc cgtgccagtt catcgcgggc
     8941 attccacagc gtcttcagat cggtcagcgg ccgcaccgtg ggagtgacga tcttgatgtt
     9001 gctgcaatcg gccgtgacgc cgctgtagcg gcgaatcttg gtgccggtcg gcaggttgac
     9061 cgaatcgaag aagatctcca ccaccgggtc gacatcgcag ccatcgagcg tggcattgcc
     9121 cgtggtcggg gaattgccac cgaaatcctc ccgcagccgg ccctgtccat cgatgaagag
     9181 cccatgcagc gtgccggccc aggtgatgct gttctgcccc gcctcgatgc gcggatggta
     9241 gagcgcctga tagaccgccc cctcgccgga gacattgttc gccaccaccg ccgccgcggt
     9301 gccggaggcc accgagccgg ccaccgaggc gaagatgcgg ttcagcgccg cctccagact
     9361 gctggggctg cgcagttgaa agtagttgtc gggcacgcca tcggatcctg cggcaccggt
     9421 caggttgttg acactgtccc actcgcgtgg gtcgtttggc gagttgctgc tgtcgaggtc
     9481 attgaagccg ccatacttgg ccgccagcca cagtggtttc tgcagggtgg tgccgccacc
     9541 ggtgatcgac agattttgaa agcctgatgc cgtgccgccg ccgttgctgc cctgcgcaac
     9601 cgtgatctct tttggatagt tcatgggctg ctgtggatcg gtgcccacca aggtaaagcc
     9661 catatcgaag gcggcatcgg cctggtctct ggtcacgacc gtcctgacgt gaagtgtgcc
     9721 gttcacctcg caatactcca gccgctgttc cgcgtcgaag tcgtagtcac tgccccaggc
     9781 ggtgccctcc caggtcagca gaaagcggtg gtaactgcca ttgttggtgc agggcgaggg
     9841 gagtgtggcg ggggcggggg gcggttcgct gaccacatcg atcagtgagc acttctgcaa
     9901 gaggtaggga gcgtttccgt tggcagagta ttccgacctg cacagcggca cgatctgcgc
     9961 ctgcttcgtg ccgctggtga cggtgaagct gggcaggtcc tcggccaccg agaccgcgaa
    10021 ggtcttgatc ttctgggtgc cattgaggcc agtacgcaga tccttgatcg ccgcctcgta
    10081 ggccagtccg gcgatgccgt agccgccctc ggcgctgggg gcctcggggc agatgccatc
    10141 ggcctgatcg agcgtgccgg ccgccagcgt cttggggctg cactcctggt cggtactgct
    10201 gccaacccgg ccggccagca cgctggcgcc atccagcccc tccttgttct gcacggcatt
    10261 ggtggccgtg gccacggcga atccagtcgg cagaccgctg gagggccagt cggctgcatc
    10321 gcgcatcgag tcgaacgaac cctgcgaggt ggagatcagc agcaccgcac actgggcgca
    10381 gggcacggca ctgttgccgg gaccgaagtt gtaggggtcg ctccaactgg tcagccgggt
    10441 cagcccggga atcatggctg cttcagttgg cgctggactg taccgggagg gcgaaggcga
    10501 actagtgaaa gccgcagagg cggcactggc gccggccaga tagcgcaccg cttcgaggta
    10561 gagctcaccg agcgggttgc cccagtcggc gcaggagcca tccttgacgg ctgcggatga
    10621 gacgtagcta ttgcagggtg gatagcgcag aagggatgag gtcgagaagt ccatctgata
    10681 ggtggcgatg cgcagtttgt cgagcgtgcc gatcaggctg ccggcagtga tctggccggt
    10741 ggcaacattg acctcgctga gcaggccgac cccctggcgc agcacaccac catggctgtt
    10801 gttgtcgtag ctgccggtca tcagcccgaa gcgcatctgg ccgcgctcgc catagcgttg
    10861 cagcagaccg atcggcttga acaggtcatc gctggcattg tttggcgtgc cgttgtcgta
    10921 gcgcttgcag cgggcggaga gcggcccatc ctggccggcg acgcagcgct ccaccagcac
    10981 ctggtaggcg ttgctggtgt cattgccaac ggggcgatcg ggctgggcaa gagcgctgtc
    11041 ccggtgggtg gtgccatgcc actgacactg cgccacctcg gtcgaggccc agttgggata
    11101 gccaaccttg ccggccgcca cattgccata ggccactcgc agcagggggg ggtagctggt
    11161 ggtgttgagg gtgccgctca gattggtcga agccggccac tgggagacat tgcacagcgt
    11221 gatggcgcca ttggtcggac tgccttccac caggtcggcc agcgcgatgg cgccgctggc
    11281 ggcgccgtca tagaccttgg caaaggcatg gatgtcgggc gggatcagct cgcgctccag
    11341 cagcaccgcg ccactgctgg tgttgggatc gaccctgcgt ttgccgccat agagcacggt
    11401 gcggatcagg tcgatgcggg tcatgctggc ccagttgaga aagttgccac tccagccatc
    11461 gacagtgctg catttgtgag tcccactgtt gacctgcccg atcggagaaa acttgccgtt
    11521 gtcgtactga taacagaagt tgctgtcgaa atagccgaga tactcgatgc tgtccttgta
    11581 ggtggtgtca atctggccat cgccatcgat gtcgctgtag tcggagtagg ccttgcggaa
    11641 cagctcgtgg tcgcgcgaca tcgcgatcag cagcagcggc ggggtggcct cgaccgcggt
    11701 gaccggcagg ctggtgtagt tcgaggggtt gccggtgggg gcggcgaaca gcggtgcgct
    11761 cagcagcagg gtcagcaggg tcagcaggcg tgacagtgcg gcgggcatgg gggtgctccg
    11821 gtcatttgac ataggtggat tgcagcatca cctgggcgcg gtcggtgccg ccgccggcca
    11881 gcgcggtgat gcggtagacg cgctggcagc cggcgttggc cgggtcgaac ggatggccgg
    11941 cgcagtcctc gggtggcgcc gtcgccgggc gggcgagccc ggccagttcg atgatgtagc
    12001 gggccggtgc cctgacgccg ggcaggttgg cgccgtaggt gaggtggcgt ccggcgttgt
    12061 tccagacatc gacgctgccg accggcgtgc cgctgtcgca gaccggcagt tcccagcgtt
    12121 cgtagaggtg gcccgcctgg tgacaggcct tgccgaggct ggcggcatgg ccggtgtcga
    12181 actgtctgga ctggcagtag ccgcccgtgc agctgttgcc gccggctttg atgcgggcgt
    12241 ccagcagggc ggaagagctg gagtggatgg tgcgttcgcc ctcgcgcagt gccgcctcgg
    12301 ccgactggat cgccagctcc tggtcgcggt agtggccggt catgcgcgcc tccagcaggc
    12361 tgccgcccat gctggccagc cccatgacgg tgaggatcag cagcaggatc aggctgacca
    12421 gcaggatgat gccgcgctgg cgcgctgggg tgccgaaggt cgactggagt cggtcagcca
    12481 tcgaaacgcc cccggttgcg cagcttgatg acggtgctga ccaccttgcg cagcttgcgg
    12541 tcggcgaggc tggtgaactg ggtgccggcc aggtcgaagg tctcggccgc cggttggctc
    12601 ttgatgtcgt cggccgagac cagcagcagc gcgatctcga tggtgaccac cgggttgctg
    12661 ccgaccgcat cggcgctgac ctggctgttg gccacgccgt caccatcgct gtcgatgccg
    12721 tagcgcagtt gcaggctctc gaccccttcg gccagctcct cggcatcctc accggtttca
    12781 tcgcggtaga ccttgcggta gagcgctggc tgcccggccg ggttgcggcc gatgtagaac
    12841 accgtgtttt tgacgccata gagctcgctg tactgggtga tcggggtcga ccagacagcg
    12901 gcacggttgc cgggggtcag cgcagtgccg gtcgcgttgt tgcagggtga cgtgcggtcg
    12961 aggtcgcggt tggtgatgct ggcgccgtcg tcacagttct ggaacaggtc ggcgctgacg
    13021 cagctcgaca gcagcagaaa gaggttgcgg cggctctttt tctgcgcggc ggtcgaggtg
    13081 gcaaaggtct tggcctcgtt gtcggggttg aggtcccact cgctggccgg atgcagagcg
    13141 accttggggt ggtccggggc gatcacgccg ccgaccagcg cgtcgatgcg ctggtcggcg
    13201 cgcttgacca ccagcaggtc gctgccctgg accacg
//
LOCUS       DAORCQ010000066        12661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3238_length_12661_cov_5.580835, whole genome shotgun sequence.
ACCESSION   DAORCQ010000066 DAORCQ010000000
VERSION     DAORCQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_12661_cov_5.580835"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKI23_07945"
     CDS             <1..261
                     /locus_tag="PKI23_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN36908.1"
                     /translation="RVRFDRLTPSEKRYLRAMAELGPGPHRSGDIAQVLHKKVTSLAP
                     IRNQLIAKGMIWSPSHGDTAFTVPMFDAFMRRIMPGDEWRSE"
     gene            complement(493..1122)
                     /locus_tag="PKI23_07950"
     CDS             complement(493..1122)
                     /locus_tag="PKI23_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36909.1"
                     /translation="MFRSMLLAVAAALALPAAADITAVYAMAGGEQLRVEYRDAANFR
                     IGTDSRNYQLMLDGRLYAVAQGQVIDVDKMSRQIKAVGAETFLAGVLGNATADVPTDV
                     SMRPLGRKETVAGQTGEVYEAVARTANGEQRADLVVTRNPDLTAVQNALLKVAKEAVA
                     AVGAESSAYTRPLQQVEQQRLGGLLRYGKQLKLVSLDRAPIPDGRIAMP"
     gene            complement(1152..2573)
                     /locus_tag="PKI23_07955"
     CDS             complement(1152..2573)
                     /locus_tag="PKI23_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNN36910.1"
                     /translation="MRHRLWALALAAPLAATVQAAPLRSTLDDQKALAVTVYNGDLAL
                     VKDTRELTLPAGESVLELRDVSAQIRPETALLRVAKGNPIALLEQNFDFDLLTPAKLL
                     EKYIGREVRVVRTHPTTGADSTETARVLAAGEGVVLKIGDRIETGLPGRLVFDAVPDN
                     LRDRPTLAVTVNGDGAGQRALELSYLTGGLTWQADYVAELAANEKELALSGWVTLTNR
                     SGTAYRNARLQLVAGDVNRVEPALRRGRVAETMMAKSDAGALREESLLDYHLYTLGRP
                     TTLADQQTKQVALLAAPAVQVQKEYHLDGAEYYYGTRLGEVDTPHKVAVLLHVDNRKA
                     AGLGLPLPAGVVRVYQRDSAGGVQFIGEDRIDHTPENETAKIKLGDAFDVTARRTQTD
                     FKVLTVDKGERPRFESAWRLDFKNARKVPVVITVDEPLPGDWEIVDDSQPHQKFGAHG
                     ARWRVTIPPGGGQTLTYRVRVRM"
     gene            complement(2739..3365)
                     /locus_tag="PKI23_07960"
     CDS             complement(2739..3365)
                     /locus_tag="PKI23_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNN36911.1"
                     /translation="MTAHHRHPALILLLALLLSGCTGWLQGQRPPPQTPHDLGWLPPP
                     SAIELREVPLLAVDAPPWLNDLAVRYRQSGTDETALHSYPDDQWLAAPRELLLDRLQQ
                     TLLAAHPAAADPQSPRYRLEVRLLRFEQEYTGPSATAVVALQAVLKEPRGRRQWASPL
                     LEGRVASATGPAGAVRGLGQATDQAIGRLLAWLAETQLRHAALAEPSP"
     gene            complement(3362..4282)
                     /locus_tag="PKI23_07965"
     CDS             complement(3362..4282)
                     /locus_tag="PKI23_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNN36912.1"
                     /translation="MENRAHALLTGLFVIGLGLAAALASFWLHDTRQARTPYLLITQG
                     AVSGLVPYSTVRYRGVEIGQVTTVDFAHDGTRDIHIRIEIQPDIPITANTYATLRYMG
                     ITGLAQVELADDGPPDAPRLPSSSASLARIPMRPSLFESLSDAGQNVLTQFQQLLVQL
                     QQLLGPENQSSFRQTMANVEVTTRKLAEFTQRLEPMVQRLPKISEDGSQTMAHVEQIA
                     RELEATLVQVRQTTARLDAVSSLADNTAETLLVETLPATQSTLQEIEAAARNLERLGD
                     ELRRDPSSLILGRGRAPEPEPSTSQRSHAP"
     gene            complement(4288..5064)
                     /locus_tag="PKI23_07970"
     CDS             complement(4288..5064)
                     /locus_tag="PKI23_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN36913.1"
                     /translation="MSRDSAPVIELRGIVTRFGSQLVHDGIDLTVRRGEVLGLVGGSG
                     SGKTTLLRQMIGLQRPSAGRVIAFGEPLFDLRGSAAARLRQRWGVLFQYGALFTALTV
                     AENVALPLRELRRLDEAMIRELVRLKLAMVGLKPEDGLRYPAELSGGMVKRVGLARAL
                     ALDAELLLLDEPTSGLDPVASELFVDLIAELRGVLGLTVVLVTHDLDTLVDLCDRIAV
                     LAERKLVVVGTLDEVVACDHPFIRSYFHGRRGGRLLDTRR"
     gene            complement(5061..6188)
                     /locus_tag="PKI23_07975"
     CDS             complement(5061..6188)
                     /locus_tag="PKI23_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN36914.1"
                     /translation="MPIDDTPRLHVEAQNGQRRILLSGQWVLRHLSATTLGRLREQLA
                     ACGRSADALWDLETLDGLDSSGALLLWQQWGERRPSQLRLRPEHEPLLLRLAQLPPAV
                     QPPRRHLLAPVTRLGAAVLSGAVHLRELIALLGQLTLDLLGIVTRPTRIPWREISASI
                     HKSGGRALLITGLVGLLIGVVLSYLSALQLRLLGGERFIVNLMGLTVTRELGPMLAAI
                     LIAGRSGSSITAELGVMRLTQELDALRTLGISPFKRILLPKVIALALVMPLLAFWTMA
                     AAIFGGMVVAQNELDISFGQFLTDLPAAIPVATYWIALIKSVAFGVVIGLVACHFGLR
                     IKPNTESLGYETTNAVVTAITLVILADALFAIALRDVGWSE"
     gene            complement(6261..7487)
                     /locus_tag="PKI23_07980"
     CDS             complement(6261..7487)
                     /locus_tag="PKI23_07980"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNN36915.1"
                     /translation="MKPGRAANHQQGAILVLNAGSSSLKFSLFQPDEAVALQRIEEGE
                     ISGIGHEPRWRLRTGAGQVTDERSLTPAEAGEMEAAIGLLCGWLRGRLADAALLAVGH
                     RVVHGGVDHAAPVRVDAALLATLERLIPLAPLHQPHNLAAIRAIEALWPQVPQVACFD
                     TAFHRSHSQLADLYALPWEHYVAGVRRYGFHGLSCEYIADRLHQVAPAIAGGRVIVAH
                     LGSGASLCALRAGRSIDSTMGFSPLDGLPMGSRCGAIDPGVLLYLAGERGMSFEELER
                     LLYRQSGLLGLSGLSGDMRTLLASSEPRAQLAVDHFVQRTAREIGALTAMLGGVDALV
                     FTGGMGENSPLIRQRIVTASAWLGMALDETANRRGAACITAAGSPVSAWCLPTDEALV
                     IARHTRAVVLDVEPAG"
     gene            complement(7498..9141)
                     /gene="pgm"
                     /locus_tag="PKI23_07985"
     CDS             complement(7498..9141)
                     /gene="pgm"
                     /locus_tag="PKI23_07985"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNN36916.1"
                     /translation="MSIHPLAGTTAPPAMLVDLGKLRDAYASLMPDVNDPAQRVSFGT
                     SGHRGSSLRGSFNEGHVLAISQAICDYRGQQGISGPLFIGRDTHALSEPAFGTALEVL
                     VANGVETMVDQAGGYTPTPAISHAILCHNRGRDSGLADGIVITPSHNPPEDGGFKYNP
                     PHGGPADTGITRWIEERANWHLANGLKAVSRIAEARARQAVHSHDYVGRYVDDLAAVV
                     EMDTIRASGLKIGADPLGGAGIAYWGAIVERHGVQIEVVNQRIDPAFGFMPLDWDGKI
                     RMDCSSPHAMAGLIALRERFDIAFGNDTDYDRHGIVTRAGLMNPNHFLTAAIHYLFTH
                     RPHWRADAAIGKTVVSSSMIDRVAGRIGRRLLEVPVGFKWFVDGLRDGSMGFGGEESA
                     GAAFLRRDGTVWSTDKDGLIMDLLAVELMARCGRDPAELYADLTAELGAPLYARIDAP
                     ATAAQKATLQKLSPRDVEAHQLAGDPIQAMLTTAPGNGAAIGGLKVVTAQGWFAARPS
                     GTEEVYKLYAESFRGSEHLARIQDEAQAIIARAFAAAAK"
     gene            complement(9308..11701)
                     /locus_tag="PKI23_07990"
     CDS             complement(9308..11701)
                     /locus_tag="PKI23_07990"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNN36917.1"
                     /translation="MPPALEQPDPANPLSMQELALIDAWWRACNYLSVGMIYLRDNPL
                     LREPLRIEHVKHRLLGHWGASPALSFVWTHLNRLIRRDALDVLFMAGPGHGAPGVLGP
                     TYLEGTYSEIYPDKSQDVEGLRRFFKQFSFPGHIGSHVTPETPGSIHEGGELGYSLAH
                     AYGACLDNPQLIVACVVGDGEAETGPLATAWHSNKFINPARDGAVLPILNLNGYKIAN
                     PTLLARISRDELDALFRGYGYTPHFVEGDEPQSMHQKMAATLERAIGEIRAAQQAARN
                     GGIIERPRWPMIVLRSPKGWTGPKQIDGHQAEGSWRSHQVPLTDVRKDPVHLQQLESW
                     LQSYRPQELFDANGTLRPELRALAPEGDRRMSANPHANGGVLRKALKLPDFRHHAVDV
                     TQPGHLLYENTRPLGTFLRDILRDNPTNFRLFGPDETASNRLQAVYEATKKVWMAEIY
                     PEDADGGELSRDGRVMEMLSEHTLIGWLEGYLLTGRHGLFHTYEAFAHVIDSMFNQHA
                     KWLDICKNHVPWRASVASQNILLSSTVWRQDHNGFSHQDPGFIDLVTNKSPSVTRIYL
                     PPDANTLLVVADHCLRSTDCINVIVADKQQHLQFTSIDEAIVHCAKGIGIWARASNDD
                     GVEPDVVMACCGDVATQEALAATAILREQLPDLRVRFVNVVDLYRMQPASEHPHGSTE
                     RDFDSLFTTDRPVIFNFHGYPWLIHKLAYRFKNHENLHVRGYKERGNINTPLELAILN
                     QTDRFTLVMDVIDRVPGLAVRAAHLKEQMKNEIILNLAYAHEHGTDPVHITDWVWPY"
     gene            complement(11760..12503)
                     /locus_tag="PKI23_07995"
     CDS             complement(11760..12503)
                     /locus_tag="PKI23_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HNN36918.1"
                     /translation="MPSQANRHRCEIVEVECLGATRRVILRPESLTGFDFMAGQYAQI
                     GLSGGRWCPFSIANPPHQTDHLELHIRISADSADSLEIERLIERREPLLVELPFGDCL
                     LDALPDRPTLFIASGTGFAQMKSLVEHCLHWRCQSPLYLYWGAYHPHDLYLEPLPRRW
                     QKEHPLFRYQATLFGPDARWQGRRGLLHEVIAEDFSSLAGMEVYLSGSPGMVYGTLDA
                     LLPLRLQPEQCHADAFAYAPREWSGWQRR"
BASE COUNT         2201 a         4195 c         4131 g         2134 t
ORIGIN      
        1 cgcgtccgct tcgatcgcct gacgccgagc gagaagcgct acctgcgcgc catggcggag
       61 ctgggccccg gcccgcaccg ctccggcgac attgcccagg tgctgcacaa gaaagtgacg
      121 tcacttgcgc cgatccgcaa ccagctgatc gccaagggca tgatctggag cccgagccac
      181 ggcgataccg ccttcaccgt gccgatgttc gatgccttca tgaggcggat catgccgggt
      241 gatgagtggc ggagtgagtg atgcccctgt gctcgcgggg tacgaaggcc cggccaaaga
      301 tggcgcgaat cagcagatgg ccgtgagcat gaccggttcg gccatgtcgc ccggatggtg
      361 cttctgacct tcaagccaca ggccggtcgg cccgttcgcg gtacgcacct gccctgtgag
      421 cggctcgcgc cacatcatca acgaagtgcc gggtctctcc cgcgtggtcg attcccgccg
      481 caacaccgcc tttcatggca tcgcgatgcg cccgtcgggg atcggcgcgc gatcgagcga
      541 aaccagtttc agctgcttgc cgtagcgcag cagcccgccg aggcgctgtt gctccacctg
      601 ctgcagcggg cgcgtgtagg cactcgattc ggcgccgact gcggccacgg cctccttggc
      661 caccttgagc agggcgttct gcacggccgt gagatcggga ttgcgcgtca ccaccagatc
      721 ggcgcgctgc tcaccgttgg cggtgcgggc caccgcctcg tacacctcgc cggtctggcc
      781 cgccacggtt tccttccgcc caagcgggcg catgctgacg tcggtcggca cgtcggcagt
      841 ggcgttgccc agcacaccgg cgagaaaggt ctccgcgcca acggccttga tctggcgtga
      901 catcttgtcg acgtcgatga cctgcccctg cgccacggca tagaggcggc catcgagcat
      961 caactggtag ttccgcgagt ccgtgccaat gcggaagttg gcggcatcgc ggtactccac
     1021 gcgcagttgc tcgccgcccg ccatggcgta gacggcagtg atgtcggcag cggcgggcag
     1081 ggcaagcgcc gcggcaacgg caagcagcat cgagcgaaac attggcttta tctcctctgt
     1141 gaagcaaccg gttacattct gacacgcacc cggtaggtga gcgtttgacc gccgccgggc
     1201 ggtatcgtca cccgccaccg tgcgccatgc gcgccaaatt tctggtgcgg ctgagagtca
     1261 tccacgattt cccagtcgcc gggcagcggc tcatccaccg tgatcaccac cggtaccttg
     1321 cgcgcattct tgaagtcgag ccgccaggcg ctctcgaagc gcggccgctc gcccttgtcg
     1381 accgtcagca ccttgaagtc ggtctgcgtg cgccgggcgg tgacgtcgaa ggcgtcgccg
     1441 agcttgattt tcgccgtctc gttctcgggc gtgtggtcga tgcggtcctc gccgatgaac
     1501 tgcacgccgc cggccgagtc gcgctggtag acgcgcacca cgccggccgg cagcggcagg
     1561 ccgagcccgg ccgccttgcg gttgtcgacg tgcagcagca cggccacctt gtgcggggtg
     1621 tcgacctcgc ccagccgcgt gccgtagtag tactcggcac cgtcgaggtg atattccttc
     1681 tgcacctgca cggcgggcgc cgcgagcagt gccacctgct tggtctgctg gtcggccagc
     1741 gtggtcggcc ggccgagcgt gtagaggtgg tagtcgagca gcgattcctc gcgcagggcg
     1801 ccggcgtcgg acttggccat catcgtctcg gccaccctgc cacgccgcag cgccggctcg
     1861 acccgattca catcgccggc caccagttgc aggcgcgcgt tgcgatacgc ggtgccgctg
     1921 cggttggtca gcgtcaccca gccggacagg gcgagttcct tttcgtttgc cgccagttcc
     1981 gccacgtaat cggcctgcca ggtgaggcca ccggtgaggt aggacagctc caatgcccgc
     2041 tgtccggcgc cgtcgccgtt caccgttacc gccagcgtcg gccggtcgcg caggttgtcc
     2101 ggcacggcgt cgaacaccag gcgccccggc aggccggtct cgatgcgatc gccgatcttc
     2161 agcaccacgc cctcgcccgc ggcgagcacg cgggccgtct cggtgctgtc ggcgcccgtg
     2221 gtggggtggg tgcgcacgac gcgtacctcg cggccgatgt atttctccag cagcttggcg
     2281 ggcgtgagca gatcaaagtc gaagttctgt tccagcagcg cgatcgggtt gcccttcgcc
     2341 acccgcagca gtgcggtttc gggccgaatc tgcgcgctca cgtcgcgcag ctccagcacg
     2401 ctttcgccgg ccggcagggt cagctcgcgc gtatccttca ccagcgcgag gtcgccgttg
     2461 tagacggtga cggcgagcgc cttctgatca tccagcgtgc tgcgcagcgg cgccgcctgc
     2521 accgttgccg ccagcggcgc cgccagcgcc agtgcccata gccgatgtcg cattcgtcca
     2581 ctcctgcggc cgcgccgcgt ctggtattgc tgagcccacc ctgccaccca ctgcatctga
     2641 gccaaccgga aattcggcca cgggcagaga tcagcccatg ctgtcaacgc tggacagcag
     2701 ggcaaagggt gctgggtcgt cattgtcggg gtgaagtatc agggtgatgg ctcggccaaa
     2761 gcggcatgac gaagctgggt ctcggccagc caggccagca gccggccgat ggcctggtcg
     2821 gtggcctggc cgagtccgcg caccgcgccg gcggggccgg tcgcgctggc cacccgtcct
     2881 tccagcagcg gcgaggccca ctgccggcga ccgcgcggct ccttcaatac cgcctgcagg
     2941 gcaaccacgg cggtggcgga tggtcccgtg tactcctgtt cgaaccgcag caggcgtact
     3001 tcgagccggt agcgtgggct ctgcggatcg gcggcagcgg ggtgagccgc cagcagcgtc
     3061 tgttgcaggc gatcgagcag cagctcgcgc ggggcggcca gccactggtc gtcgggatag
     3121 ctgtgcagcg ccgtctcgtc cgtgcccgac tgtcgatagc gcacggccag atcattcagc
     3181 cagggtggtg catcgaccgc gagcagcgga acctcccgca gttcgatcgc ggacggtggc
     3241 ggcagccagc ccaggtcatg cggtgtctgg ggcggcggac gctggccttg cagccaaccg
     3301 gtgcagccgc tgagcagcag ggccagcagc aggatcaggg ccggatggcg atggtgggcg
     3361 gtcatggcgc atggctccgc tgggaggtgg agggttccgg ctcgggcgcg cggccgcgac
     3421 cgagaatcag gctggacggg tcgcgccgca gttcgtcgcc gagccgttcg aggttgcggg
     3481 ccgccgcttc gatctcctgc agggtgctct gggtggctgg cagcgtctcg accagcagcg
     3541 tctctgcggt gttgtcggcc aggctgctga cggcatcgag ccgggcagtg gtctggcgca
     3601 cctgaaccag ggtggcctcc agttcacggg cgatctgctc gacatgggcc atggtctggc
     3661 tgccatcctc actgatcttg ggcaaccgtt gcaccatcgg ctccagccgc tgggtgaatt
     3721 cggccagctt gcgggtggtg acttcgacgt tggccatggt ctggcgaaag ctgctctggt
     3781 tttccggacc cagcagctgc tgcagttgca ccagcagttg ctggaactgc gtcagcacgt
     3841 tctgcccggc atcactcagt gactcgaaca gcgagggacg catcggaatg cgggccagac
     3901 ttgcggaact gctcggcaac cgtggcgcat cgggcggacc atcgtcggcc agctcgacct
     3961 gcgccagacc ggtgatgccc atgtagcgca gggtggcgta ggtgttggcg gtgatgggga
     4021 tgtcgggctg aatctcgatg cggatgtgga tgtcccgcgt gccatcgtgg gcgaaatcga
     4081 ctgtggtgac ctgaccgatt tcgacgcccc ggtagcggac ggtggagtaa ggcaccagac
     4141 cggaaaccgc cccctgggtg atcagcagat agggcgtgcg ggcctgtcgg gtgtcatgca
     4201 gccagaatga ggccagcgcg gcggccagtc caagaccgat cacgaacaga cccgtcagca
     4261 gggcatgggc tcggttttcc atggcaatta ccgccgtgtg tcgaggagtc ggccgccgcg
     4321 gcggccatga aaatagctgc ggatgaaggg gtggtcacag gccaccacct catccagggt
     4381 gccgaccacc accagcttgc gctcggccag cacggcaatg cggtcgcaca ggtcgaccag
     4441 ggtgtcgagg tcatgggtca ccagcacgac ggtcaggccg agcacgccgc gcagctcggc
     4501 gatcaggtcg acgaacagct cactggcgac cgggtcgagc ccagaggtgg gctcatcgag
     4561 cagcagcagt tcggcatcga gcgccagtgc ccgcgccagg ccgacacgct tgaccatgcc
     4621 gccggagagc tcggccggat aacgcagtcc atcctcgggc ttgagaccca ccatcgccag
     4681 cttgagccgg accagctccc ggatcatcgc ctcatcgagg cggcgcagct cccgcagtgg
     4741 cagggcgaca ttctcggcca ccgtcagcgc ggtgaacagc gcgccatatt gaaacagcac
     4801 accccagcgt tgacgcagcc tggccgccgc gctgccgcga aggtcgaaca gcggctcacc
     4861 gaaggcaatg accctgccgg cgctcgggcg ctgcaggccg atcatctgcc gcagcagcgt
     4921 ggtcttgccc gagcctgacc cgcccaccag tcccagcacc tcaccgcggc gcacggtgag
     4981 gtcgatgcca tcatgaacca actgcgaccc gaagcgggtg acgatgccac gcagctcgat
     5041 gaccggcgcg ctgtcgcggc tcattcgctc cagcccacgt cacgcagcgc aatggcaaac
     5101 agcgcatcgg ccaggatcac cagcgtgatg gcggtcacca cggcgttggt ggtctcatac
     5161 cccaggcttt cggtattggg cttgatgcgc aggccgaagt ggcaggccac caggccgatg
     5221 acgacaccga aggccaccga cttgatcagc gcaatccagt aggtggccac cggaatggcg
     5281 gccggcaggt cggtcagaaa ctgcccgaag ctgatgtcga gctcgttctg tgccaccacc
     5341 atgccaccga agatcgccgc ggccatggtc cagaatgcca gcaacggcat gaccagggcg
     5401 agcgcgatca ccttcggcag caggatgcgc ttgaacggcg agatgccgag ggttctgagc
     5461 gcatcgagct cctgggtcag ccgcatcacg cccagctcgg cggtgatcga tgaaccggag
     5521 cgacccgcga tcagaatggc cgccagcatc ggccccagtt cgcgggtcac cgtcagcccc
     5581 atcaggttga cgatgaagcg ctcaccgccc agcagccgca gttgcagggc cgacaggtag
     5641 ctgagtacga cgccgatcag cagcccgacc aggccggtga tcagcagcgc ccgcccaccg
     5701 ctcttgtgga tgctggccga aatctcccgc cacggaatcc gggtcggacg ggtcacgata
     5761 ccgagcagat ccagcgtcaa ctgccccagc agggcgatca gttcacgcag atgaacggct
     5821 ccgctcaaca cggccgcgcc gagtcgggtc accggcgcca gcagatggcg tctgggtggc
     5881 tgaaccgctg gcggcaactg cgccagccgc agaagcagtg gttcatgctc agggcgcaac
     5941 cgcagctgtg atggacgccg ttcgccccac tgttgccaga gcagcagtgc accgctgctg
     6001 tcgagaccgt cgagcgtttc gaggtcccac agcgcatctg cactgcgccc acaggccgca
     6061 agctgctcgc gcagccggcc gagcgtggtg gccgacagat gacgcagcac ccactgcccc
     6121 gagagcagga tgcggcgctg gccgttctgc gcttcgacat gcaggcgcgg ggtgtcgtcg
     6181 atcggcattg tcagaggtca actccagaag cgcggcccac gcagtgtggc accggttgcg
     6241 gccaccattc tgccactgtt tcacccggcc ggttcgacat ccagcaccac cgcccgggtg
     6301 tgccgggcga tcaccagcgc ttcatcggtg ggaaggcacc aggccgacac cgggctgccg
     6361 gcggcggtga tgcaggcagc gccgcgccga ttggccgtct cgtccagcgc catgccgagc
     6421 caggccgagg cggtgacgat gcgttggcga atcagcgggg agttctcgcc catgccgccg
     6481 gtgaacacca gcgcatccac gccacccagc atggcggtga gcgcgccgat ctcccgggcg
     6541 gtgcgctgca cgaagtggtc gaccgccagt tgcgcgcgcg gttcactgct cgccagcagc
     6601 gtgcgcatgt cgccactgag gcccgacagc ccgagcagac ccgactgccg atagagcagt
     6661 cgctccagct cttcaaaact catgccgcgt tctcccgcca gatagagcag cacaccgggg
     6721 tcgatcgcgc cgcaacgact gcccatcggc aggccatcga gcggcgaaaa acccatggtc
     6781 gaatcgatgc tgcggcccgc ccgcaacgca cagaggctgg caccgctgcc gaggtgggcg
     6841 acgatcacgc gccccccggc aatggccggt gccacctgat gcagccggtc ggcgatgtac
     6901 tcgcaagaga ggccatgaaa gccgtaacgg cgcacgccag ccacataatg ctcccacggc
     6961 agtgcgtaga ggtcggccag ttgtgaatgg ctgcgatgaa aggcggtgtc gaaacaggcc
     7021 acctgcggaa cctgcggcca cagcgcctcg atcgcacgaa tcgccgccag gttgtgcggc
     7081 tgatgcagcg gcgccagcgg aatcagccgc tccagtgtcg cgagcagcgc cgcgtcaacc
     7141 cgcaccggtg cggcatggtc gacgccgcca tgcaccaccc ggtggcccac ggccagcagc
     7201 gctgcatcgg ccagccgccc gcgcagccag ccgcacagca gcccgatcgc ggcctccatc
     7261 tcgcctgcct cggccggcgt caagctgcgc tcatcggtca cctgaccggc gccagtgcgc
     7321 agcctccatc gcggctcatg gccgatgcct gagatctcgc cctcttcgat ccgttgcagg
     7381 gccacggctt catcgggctg gaacagcgaa aacttcaggc tggaggagcc ggcattgagc
     7441 accaggatcg caccctgctg gtggttggcc gccctgcccg gtttcacgct gctgccatca
     7501 cttcgccgct gcggcaaatg cccgggcgat gatggcctgt gcctcgtcct ggatgcgcgc
     7561 caggtgttcg ctgccacgaa aactctcggc atagagcttg tagacctctt cggtgcccga
     7621 cggccgcgcg gcgaaccagc cttgggcggt caccaccttg agtccgccaa tcgccgcgcc
     7681 attgcccggt gccgtggtga gcatcgcctg aatcggatcg ccggccagtt gatgggcctc
     7741 gacatcgcgt ggcgacagct tctgcagcgt ggccttctgt gccgccgtgg ccggcgcatc
     7801 gatgcgggca tagagcggcg cgcccagctc ggcggtgagg tcggcataga gttcggccgg
     7861 gtcacggccg caacgggcca tcagttccac cgccagcagg tccatgatca ggccatcctt
     7921 gtcggtgctc cagaccgtgc catcgcgccg cagaaacgca gcccccgcgc tctcctcgcc
     7981 accgaagccc atcgagccat cgcgcagtcc atcgacgaac cacttgaagc cgaccggcac
     8041 ctccagcagc cggcggccga tccggccggc cacccggtcg atcatgctgc tgctgaccac
     8101 ggtcttgccg atcgccgcat cggcgcgcca gtgcggtcgg tgggtgaaca ggtagtgaat
     8161 cgccgccgtc agaaagtggt tgggattcat caacccggca cgggtgacga tgccgtggcg
     8221 gtcgtagtcg gtgtcgttgc caaaggcgat gtcgaaacgc tcccgcagcg caatcagccc
     8281 ggccatcgca tggggcgacg agcagtccat acgaatcttg ccatcccagt cgagcggcat
     8341 gaagccaaag gccggatcga tccgctgatt gaccacctcg atctgcaccc catggcgttc
     8401 gacgatcgcg ccccagtagg cgatgccggc gccccccagc ggatcggcgc cgatcttcaa
     8461 gccagatgcg cggatcgtgt ccatctccac caccgccgcc aaatcatcga catagcgtcc
     8521 cacatagtca tgggaatgga ccgcctgtcg tgcccgcgcc tcggcgatgc gcgacaccgc
     8581 tttcagcccg ttggccagat gccagttggc ccgctcctcg atccagcggg tgatgccggt
     8641 gtcagccggt ccgccatggg gcgggttgta cttgaagccg ccatcctccg gcggattgtg
     8701 cgaaggggtg atgacgatgc catcggccag gccgctgtcg cggccacggt tgtggcagag
     8761 aatcgcatgg gagatcgccg gcgtgggggt atagcccccc gcctggtcga ccatcgtctc
     8821 gacaccattg gcgaccagca cctccagcgc ggtgccgaag gccggctccg acagggcatg
     8881 ggtgtcgcgg ccgatgaaga gcggcccgct gatcccctgc tggccgcggt aatcgcagat
     8941 cgcctggctg atcgccagca catggccttc gttgaagctg ccgcgcagtg acgagccgcg
     9001 gtgaccgctg gtgccgaagc tgacccgctg cgccgggtca ttcacatcgg gcatcagtga
     9061 agcgtaggcg tcacgcagct tgccaagatc gaccagcatg gccggtgggg cggttgttcc
     9121 tgccagggga tgaatggaca tggtgtgact ctgtcggtgg aggatgttga ggtggtgcgg
     9181 ttgccgggtg gatgtgcgcc tgagcgaatc aatcagttcg caggcacatt gtatgattca
     9241 ggaaagcgct cgccattgag aggaatcaga tgcgatggcg aacagccacc cacactgctt
     9301 gcctcattca atagggccac acccagtcgg tgatgtgcac cggatcggtg ccatgctcat
     9361 gggcataggc cagattgagg atgatctcgt tcttcatctg ctccttgagg tgggcagcac
     9421 gcaccgccag ccccggcacc cggtcgatca catccatcac cagcgtgaag cggtcggtct
     9481 ggttcaaaat cgccagctcc agcggggtgt tgatgttgcc gcgctccttg tagccgcgca
     9541 catgcagatt ttcgtggttt ttgaagcggt aggcgagctt gtggatcagc cagggataac
     9601 catggaagtt gaaaatcacc ggccggtcgg tggtaaagag gctgtcgaag tcacgttcgg
     9661 tcgaaccatg tggatgctcc gaggctggct gcatccggta gaggtcgacc acattgacga
     9721 agcgcacccg cagatcaggc aactgctcgc gcaggatggc ggtggccgcc agcgcctcct
     9781 gggtggcgac atcgccacag caggccatca ccacatccgg ctcgacgcca tcatcgttgc
     9841 tggcccgcgc ccagatgccg atgcccttgg cgcagtggac gatcgcctcg tcaatgctgg
     9901 tgaattgcag atgctgctgc ttgtcggcaa cgatgacatt gatgcagtcg gttgaacgca
     9961 gacagtggtc cgccaccacc agcagggtgt tggcatcggg tggcaggtag atgcgggtga
    10021 cgctcggact cttgttggtc accagatcga tgaatcccgg atcctgatgc gagaaaccgt
    10081 tgtggtcctg ccgccagacc gtggaagaga gcaggatgtt ctgcgaagcc accgatgcac
    10141 gccaaggcac atggttcttg cagatgtcca gccacttggc gtgctggttg aacatcgagt
    10201 cgatgacgtg ggcaaaggcc tcataggtgt gaaagaggcc atgccggccg gtcagcagat
    10261 agccttcgag ccagccgatc agcgtgtgct ccgaaagcat ctccatcacc cgcccatcgc
    10321 gtgacagctc gccgccatcg gcatcctcgg gatagatctc cgccatccag accttcttgg
    10381 tcgcctcgta gaccgcctgc agccggttgg aggcggtctc atccggacca aacagacgaa
    10441 agttggtcgg gttgtcgcgc aggatgtcgc gcaagaaggt ccccaatggc cgggtgttct
    10501 cgtagagcag atggccgggc tgggtcacat ccaccgcatg atggcgaaaa tcgggcagct
    10561 tcagcgcctt gcgcagcact ccaccattgg catgcggatt ggcgctcatg cgccggtcac
    10621 cctccggggc cagtgcgcgc agctccggcc gcagcgtgcc gttggcatcg aacagctcct
    10681 gcggccggta gctctgcagc cagctttcga gctgttgcag atggactgga tccttgcgca
    10741 catcggtcag cggcacctgg tgcgaacgcc acgatccctc ggcttggtgg ccatcgatct
    10801 gcttcgggcc ggtccagccc ttcggcgagc gcaacacgat catcggccag cgtggccgct
    10861 cgatgatgcc gccgttgcgc gccgcctgct gggccgcgcg aatctcgccg atcgcccgct
    10921 ccaaggtggc cgccatcttc tggtgcatcg actgcggctc atcgccctcg acgaagtgcg
    10981 gggtgtagcc gtagccacgg aacagcgcat cgagctcatc acggctgatg cgggccagca
    11041 gcgtcggatt ggcaatcttg tagccgttga ggttcagaat gggcagcacc gcgccatcgc
    11101 gcgccggatt gatgaacttg ttggagtgcc aggctgtggc cagcggcccg gtctccgcct
    11161 cgccatcacc caccacacag gccacgatca gctgcggatt gtcgaggcag gcgccatagg
    11221 catgtgccag actgtagccc agctcgccac cttcgtggat cgagcccggc gtctccggcg
    11281 tcacatgcga accaatgtgg cccggaaacg agaactgctt gaagaagcgg cgcagccctt
    11341 cgacatcctg gcttttgtcg gggtagattt cggaataggt gccctccaga taggtcggcc
    11401 ccaacacccc gggcgcccca tggcccggac ccgccatgaa gagcacatcc agcgcatcgc
    11461 gcctgatcag acggttcaga tgggtccaga caaaggagag tgccggactc gctccccaat
    11521 gaccgagcag gcgatgcttg acatgctcga tccgcagcgg ttcgcgcaac agcggattgt
    11581 cgcgcagata gatcatgccc accgacaggt agttgcaggc gcgccaccag gcatcgatca
    11641 gcgccagctc ctgcatgctc aacggattgg ccggatcggg ttgctccaag gctggcggca
    11701 tcatcttcgt acctctgtga cagttgccca gtatcggcaa ctatcctacc tgaacgaatt
    11761 catcgccgct gccaccccga ccattcacgc ggtgcatagg cgaatgcatc ggcgtggcac
    11821 tgttcaggct gaagcctcag tggcaacagc gcatccaagg tgccgtagac catccccggt
    11881 gatccgctca gatagacctc catccccgca agcgacgaga aatcctccgc aatcacctcg
    11941 tgaagcagcc ccctgcggcc ctgccagcgt gcatcggggc caaacagcgt cgcttgatag
    12001 cgaaacagtg gatgttcctt ctgccatctc cgcggcaggg gttccagata gagatcgtgg
    12061 gggtggtagg ccccccagta gagatacagt ggcgactggc agcgccagtg aaggcagtgc
    12121 tcgaccagcg acttcatctg ggcaaaaccg gtgccggaag ctatgaacag cgtcggccgg
    12181 tctgggaggg cgtcgagcag acagtcgcca aacggcagtt ccaccagcag cggttcgcgc
    12241 cgttcgatca gccgttcgat ctccagcgaa tccgcggaat cggccgagat gcgaatgtgc
    12301 agttcgaggt gatcagtctg gtgcggtgga ttagcgatcg agaacggaca ccagcggccg
    12361 cccgaaagtc cgatctgcgc atactgtccg gccataaaat cgaaaccagt gagcgattca
    12421 ggccgcaaga tcacccggcg ggtggcgccc aaacactcga cctcgacaat ctcacagcga
    12481 tggcggttcg cctgactggg catgctgccg tatcctcgcg cagaatcgct ccacaccgaa
    12541 acatggtcac tccagtttcg atatggagtc gtagctgttt tagtggccta ttttccactc
    12601 caaaaaacaa aagccccgtt gttgaagcgg ggcttttgtt ttttgggagc ctgacgatga
    12661 c
//
LOCUS       DAORCQ010000067        12522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3298_length_12522_cov_5.673057, whole genome shotgun sequence.
ACCESSION   DAORCQ010000067 DAORCQ010000000
VERSION     DAORCQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12522
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3298_length_12522_cov_5.673057"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKI23_08000"
     CDS             complement(<1..594)
                     /locus_tag="PKI23_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNN36919.1"
                     /translation="MKVTARTALPTLLGCLLSLGLQAENLAEVYQNAIDNDHRLKAAR
                     ATYQAQHEIVFQARGVLAPDVRLDGSATRSYVDSQASDYKVHINGHGYGLKLNQPVFN
                     LQAWYGYKQAQAIDLRAAADLSAAEQDLIIRTAAGYFDVLRASDDLRSVQAEETAVKR
                     QLDQVSERFNVGVSAITDVQEAQAHLRELGLHIDVTNL"
     gene            740..2614
                     /gene="thiC"
                     /locus_tag="PKI23_08005"
     CDS             740..2614
                     /gene="thiC"
                     /locus_tag="PKI23_08005"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNN36920.1"
                     /translation="MSSSREIPASDAMRLPAQSTRPLVGSTKQYRVGSRDDLRVPCRE
                     ITLSPTGTTANPPLRVYDSSGPYTDTTQQIDLGAGLPPLRADWISARGDSEPLAAFSS
                     RYARQRQNDLKTAELRFPQPPLPRRARTGSNVTQLGHARRGIITPEMEFVALRENLYL
                     EQAREQGLLQRQHRGRSFGAEMPLEVTPEFVRQEVARGRAIIPANINHPELEPMVIGR
                     NFLIKVNANIGNSAVTSSIAEEVDKLVWAIRWGADTVMDLSTGKHIHETREWIIRNSP
                     VPIGTVPIYQALEKVDGKPEELSWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAN
                     RLTGIVSRGGSIMAKWCLAHHQENFLYTHFDEICEIMKAYDVSFSLGDGLRPGSIADA
                     NDAAQFAELETLGELTRIAWRHDVQTMVEGPGHVPMQLIKENMEKQLECCDEAPFYTL
                     GPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPDREDVKQGLIAYRIA
                     AHAGDLAKGHPGAQLWDDTLSKARFEFRWEDQFRLALDPDTACAYHDATMPKESGKVA
                     HFCSMCGPKFCSMQITQQVREYARERGLDGPDAAIDSGLAEKASEFRAQGGELYGNTG
                     "
     gene            2626..3279
                     /locus_tag="PKI23_08010"
     CDS             2626..3279
                     /locus_tag="PKI23_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNN36921.1"
                     /translation="MQQFSHDDVELLGRELLHQGFFRLERFTLRHRLFGGGWSPPLRR
                     ELLLRRPAVALLPYDPLRDEVVLIEEFRVGALDPALLADDVEPARRRQAASPWLIELV
                     AGITEPDEPIEATAHREAHEEAGLTLFELLPIADYLSSAGGSDERLHLFCGRVDSSQA
                     GGIHGVDHEAEDILVRAVARETAYGWVEQGLIRNAATLVALQWLQLNHARVRAQWQC"
     gene            3267..3743
                     /locus_tag="PKI23_08015"
     CDS             3267..3743
                     /locus_tag="PKI23_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HNN36922.1"
                     /translation="MAVLTATPAPVAGRRRYRVDLVAQQAQAASNYQRLLRLLPELHQ
                     RTYRELLLRERLVRFVITHPSRYTSFVTIHHDQPRPPWLAPWCMELSLYHDAGLAEVV
                     RCMDQRAVAAVHDYPNRRMHQVDEKWQLNFFLGEWLRFCLQQGRATACELPFLPAG"
     gene            3747..4535
                     /gene="cpdA"
                     /locus_tag="PKI23_08020"
     CDS             3747..4535
                     /gene="cpdA"
                     /locus_tag="PKI23_08020"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF008359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HNN36923.1"
                     /translation="MRDTFVELVQISDSHLFADREAELLGINTDASLAAVLQRLDGRP
                     RPALLLATGDNSQDGSVAAYRRFLEQVQGRGERFVWLPGNHDLPDVMAALSPAGTLVK
                     SLDLGAWRLLLLDSTLPATVAGHLAEAEFDFVERSLCSAPQRPTLLAVHHPPCALGCA
                     WLDDQGIDNGARLLALLADHPQVRLLLCGHIHQQQARQFGPLWLLSAPSTCVQFRPDS
                     DDFAIDSLAPGLRWLRLHDDGRFETGIERVAAGLFTADHSSSGY"
     gene            4569..6455
                     /gene="parE"
                     /locus_tag="PKI23_08025"
     CDS             4569..6455
                     /gene="parE"
                     /locus_tag="PKI23_08025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNN36924.1"
                     /translation="MSDYNAAAIEVLTGLDPVRKRPGMYTDTSRPNHLVQEVVDNSVD
                     EALAGHATLIELILHADGSIEVNDDGRGMPVDLHPVEGVSGVELILTRLHAGGKFSGD
                     QYRFSGGLHGVGVSVVNALSLWLEVEVRRDGKLWRMRFESGQKVTELTEVGSAPRRVS
                     GTRVRFLPDPAFFDSPRLALPRLKQLLKAKAVLCPGLRLRFEERAEGEQIEWCYASGL
                     ADYLLESAQGATAVPPQPFTGHFEGRDEQVDWAIFWLPEGGEPIAESYVNLIPTPQGG
                     THVNGLRAGLTEALREFCEFRNLLPRGLKLTTDDLFERASYLLSARLREPQFSGQTKE
                     RLSSREAVAFISGVGKDAFSLWLNQHVEVGELLAELAISQAQRRLKASRQVARKRVTQ
                     GPALPGKLADCSSQEMGRGELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVD
                     SSEILASQEVHDIAVALGIDPGSDDLSGLRYGKVCILADADSDGRHIASLLTVLFLRH
                     FPALVRAGHLHVAMPPLYRIDLGKEVYYSLDEAEKMGILDRLSAEKRRGKPNVQRFKG
                     LGEMNPLQLRETTMAPATRRLVRLGVDGEQAAHEMLDMLFAKRRASDRRHWLERHGGT
                     AELV"
     gene            complement(6464..7660)
                     /locus_tag="PKI23_08030"
     CDS             complement(6464..7660)
                     /locus_tag="PKI23_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN36925.1"
                     /translation="MHHSPAPTVAPIPRGVWLLGLVSLCMDLSSELIHSILPLFIVTT
                     LGASPMVLGLLEGLAEATAALVKAVSGGLSDWLGRRKPLVLLGYGLSALTKPLFPLAS
                     SIGMVFTARLLDRVGKGIRGAPRDALLTDLTPATLRGAGFGLRQSLDSIGALLGPLLA
                     MGLLACFTDDVRLVLWVAVVPALLAVLLLLAVKEPPQPASAPEPPVKGTAPTGRWRAL
                     PAAFWAVTGFALLLTLARFSEAFLLLRGNSLGLPIAWAPAVMVAMNLAYVLSSYPAGR
                     LSDRIGRRRLVAMGCLLLLAADAMLALADGWWLLFGGALLWGMHMGLTEGVLSAMVAD
                     TAPAALRGTAFGLFRLTTGIALLAASLIAGALWEWVGPAATFMAGALCTALALLVLLR
                     LRPGAG"
     gene            complement(7668..8573)
                     /locus_tag="PKI23_08035"
     CDS             complement(7668..8573)
                     /locus_tag="PKI23_08035"
                     /inference="COORDINATES: protein motif:HMM:NF035938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HNN36926.1"
                     /translation="MLNEAQYRTLRQAYSEFATPAQLNWLERHERLLQQVERQRLFAS
                     SLELCEAAWRELGDSEPGAVYSLILREPRPTEIRLGQWCRADLAIALLLLLGAELAAA
                     PPARWLTKLFEAASEPVRGSGLRMLMLVDPTGSLAAWSIDAALPARFAQRQAALFRHN
                     PYPALHFPQPAFNQLVLAALSADANLLDLQNLELRLNASLSLAVVEWLQQRRDAGASL
                     PPSLWLAIRPADLDAASSRLFQAALGDGDEAHRFHAALALSRNGEVPEPLLRLAAQRE
                     DVEPVAAIRRLLRNLSRLAGPRSQN"
     gene            8721..9740
                     /locus_tag="PKI23_08040"
     CDS             8721..9740
                     /locus_tag="PKI23_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HNN36927.1"
                     /translation="MNARLFGVLLLALGCGLSLWAQGGVDRTTLAILVNDADPLSVQV
                     GQYYQQARKLEARQLIHLNFPTGKSRLSPEAFAAIRAQIERATPDFIQAYAITWSRPY
                     RVGCMSITTAVAAGFDAAFCPPEGVVCAPTRRLDYFDSSVTDPHRQLGLRPAMMLAGN
                     NLEEVKRLIDRGIAADGSHPAGSAYLLVTADRARSVRALGYGEVFRVARPPIRPRRIE
                     ADWIEHEQDVLFYFTGTAWVRQLDSNRFLPGAVGDHLTSAGGQLDGQKQMPALAWLSA
                     GATGSYGTVSEPCNFPEKFPRPAVLIDHYQRGETLLEAYWKSVASPGQGLFIGEPLAR
                     PFGER"
     gene            9858..10244
                     /locus_tag="PKI23_08045"
     CDS             9858..10244
                     /locus_tag="PKI23_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36928.1"
                     /translation="MKAQRFRLWLGGLLLGLALGCSADVFNHSSRTLFLLGEEDGKVF
                     ELPPGGAFLGRQDAILLPSGAGVRVFKTVDRIDVVVSPRHAIITYGGNLLERCGQWLI
                     GGWLDRRPVGQRWVQQIGRHYRDSFG"
     gene            10461..11057
                     /locus_tag="PKI23_08050"
     CDS             10461..11057
                     /locus_tag="PKI23_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNN36929.1"
                     /translation="MTQQVGAHKVKVGKKFELLAKALLAVVLMSTSTLVLALDPYLIQ
                     PGDVLEISVWREDGLQREALVRPDGGLSFPLVGEIGAAGQTVPAVSEEIKKRLTKFIP
                     DPVVTVALKQNLGNKVYVIGKVNRPGEFAVNRAVDVLQALSMAGGMNPFADTKNIQVL
                     RRVNGEQTSVGFNYAEMEQGKKLQQNILLQAGDVVVVP"
     gene            11054..12250
                     /locus_tag="PKI23_08055"
     CDS             11054..12250
                     /locus_tag="PKI23_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HNN36930.1"
                     /translation="MRRATFSTLLTAGALLAAGSATAGGWYQEPSVRLRTGFDDNIRL
                     RSSDPQEDFVTTLQGNGLFGYRTETESAALKLKAEANQYQQQDGLNNENLFADLNLDH
                     KGERNSFGLLTSLHRDTTLQSELETTGRVDDNLRVTRQLLQPSFEHSFAERWVGSVGA
                     SYSEVEYEDRVGSSLTDYRYVTAQTALQYRVSEDRSFTGTLFATRYTPTDFDFISRGQ
                     GVSFGWQERFSPSLTGSLSVGARKDKSERTLFGLQTEDESSGTQFDAALEKSWQRWRL
                     NLNLGRSTDPSGDGSLIQRDRLGGTASYDWNERTRLIFNANYLDIQQTNDLVSLSSND
                     RRYLTFAPGIEWRVERNWTARFDYRYRQNRFLDQGDEAESNALFVSISYLWPSPLGSS
                     GQGKPL"
     gene            12341..>12522
                     /locus_tag="PKI23_08060"
     CDS             12341..>12522
                     /locus_tag="PKI23_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNN36931.1"
                     /translation="MIGISDYLRILRRRRVLLWSVFGVVFVIAVLVTLALPSVYTSSG
                     TILIEQQEIPAEMVKST"
BASE COUNT         2201 a         4047 c         4164 g         2110 t
ORIGIN      
        1 cagattggtg acatcaatat gtaagcccag ttcccgcaga tgcgcctgcg cctcctgcac
       61 atcggtgatc gctgacacac ccacattgaa acgctcgctg acctggtcga gctgacgctt
      121 gactgcggtc tcctcggcct ggaccgaacg caggtcatcg ctggcgcgca gcacgtcgaa
      181 gtagccggca gcggtgcgga tgatcagatc ctgctcggcg gccgacaggt cggcagcggc
      241 gcgcaggtcg atcgcctggg cctgcttgta gccataccag gcttgcaggt tgaacaccgg
      301 ctggttgagc ttcaggccat agccatggcc attgatgtgc accttgtaat cgctggcctg
      361 gctgtcgaca taggagcggg tggcactgcc atcgagccgg acgtcgggtg ccagcacgcc
      421 acgggcctga aagacgatct catgctgcgc ctgataggtg gcgcgcgccg ccttcagccg
      481 gtggtcgttg tcgatggcgt tctgatagac ctcggccagg ttctcggcct gcagtccgag
      541 gctcaacagg cagcccagca gtgtgggcag agcggttctt gccgtcacct tcatggttgt
      601 ttttttcctt gctggatatc ggagttatca gtgtgatcgt tagcttgcta agcatatcaa
      661 aaaaaccgga ccatcgcgcg gctcgacagc gattgcatca tcggatagcg taaccggccc
      721 aggaccccgg accatgacca tgagctcatc acgagaaatc ccggcatccg acgcgatgcg
      781 cctgcccgcc cagagcaccc ggccgctggt tggctcgacc aagcaatatc gggtcggcag
      841 ccgcgacgac ctgcgcgtgc cctgtcggga gatcacgctt tcgcccaccg gcacgacggc
      901 gaatccgcca ttgcgggtct atgacagctc cggaccctac accgacacca cgcagcagat
      961 cgatctcggt gccgggttgc cgccgttgcg ggccgactgg atcagcgcgc gtggcgacag
     1021 cgaaccactg gccgcattca gttcacgcta cgcccggcaa cggcagaacg acctcaagac
     1081 tgcggagttg cgcttcccgc agccaccgct gccgcgccgt gcccgaaccg gcagcaatgt
     1141 cacccagctc ggccatgcgc ggcgtggcat catcacgccc gagatggagt tcgttgccct
     1201 gcgcgagaat ctctatctgg agcaggcgcg cgagcagggc ctgttgcagc ggcagcatcg
     1261 tggccgcagc ttcggcgccg agatgccgct cgaggtgacg ccggagttcg tgcgacagga
     1321 ggtggcccga gggcgggcga tcattccggc caacatcaac catcccgaac tggagccgat
     1381 ggtcatcggc cgcaactttc tgatcaaggt gaatgccaac atcggcaact cggcggtcac
     1441 ctcctcgatc gccgaagagg tcgacaaact ggtctgggcg atccgctggg gtgccgacac
     1501 ggtgatggat ctctcgaccg gcaagcacat tcatgagacc cgcgagtgga tcatccgcaa
     1561 ctcgccggtg ccgatcggca cggtgccgat ctaccaggca ctggagaagg tcgacggcaa
     1621 gccggaggag ctgagttggg agatcttccg cgacacgctg atcgagcagg ccgaacaggg
     1681 ggtggactac ttcaccatcc atgccggcgt gctgctgcgc tatgtgccgc tgaccgccaa
     1741 ccggctgacc ggcatcgtct cgcgcggcgg ttcgatcatg gccaagtggt gcctcgccca
     1801 ccaccaggag aactttctct acacccactt cgatgaaatc tgcgagatca tgaaggccta
     1861 tgatgtctcc ttctcgctgg gcgatggcct gcgtcccggt tccatcgccg atgccaacga
     1921 cgccgcccag ttcgccgagc tcgagactct gggcgagctg acccgcatcg cctggcggca
     1981 tgacgtgcag accatggtcg aggggcccgg ccatgtaccg atgcaactga tcaaggagaa
     2041 catggagaag cagctcgaat gctgcgacga ggcgcccttc tacacgctgg ggccgctgac
     2101 caccgacatc gcgcccggct acgaccacat cacctcgggc atcggtgcgg cgatgatcgg
     2161 ctggttcggc tgcgcgatgc tctgctatgt caccccaaag gagcatctgg gcctgcccga
     2221 ccgcgaggat gtgaagcagg ggctgatcgc ctaccggatc gccgcccatg ccggcgacct
     2281 ggccaagggg catcccggcg cacaactgtg ggatgacacg ctgtcgaagg cgcgcttcga
     2341 attccgctgg gaggaccagt tcaggctggc gctcgacccc gacaccgcct gtgcctacca
     2401 cgacgccacc atgccgaagg agtcgggcaa ggtggcccac ttctgctcga tgtgcggccc
     2461 caaattctgc tcgatgcaga tcacccagca ggtgcgcgaa tatgcccgcg agcgcgggct
     2521 cgatggtccg gatgcggcca tcgacagcgg tctggccgag aaggccagcg aattccgcgc
     2581 ccagggcggc gaactctacg gcaacaccgg ttgaggtcga gggtgttgca gcagttttcg
     2641 catgatgatg tcgaactgct cgggcgcgag ctgctgcatc agggcttttt tcgcctcgag
     2701 cgcttcacgt tgcgccatcg gctgttcggc ggcggctgga gcccgccgct gcggcgcgag
     2761 ctgctgctgc gccggccggc ggtggcgctg ctgccctatg atccgctgcg cgatgaagtg
     2821 gtgctgatcg aggagttccg ggtcggcgcg ctggatccgg cactgctcgc cgacgatgtc
     2881 gaaccggcgc gacgccggca ggcagcctcg ccctggctga tcgaactggt ggccggcatc
     2941 accgaaccgg atgagccaat cgaggcgacc gcccaccgcg aagcgcacga agaggccggg
     3001 ctgacgctgt tcgagctgct gccgatcgcc gattacctga gcagtgccgg tggcagcgac
     3061 gagcggctgc atctgttctg cggccgggtc gacagcagtc aggccggcgg catccatggt
     3121 gtcgaccacg aagccgaaga cattctggtg cgggcagtcg cgcgcgagac cgcctatggc
     3181 tgggtcgagc agggtctgat ccgcaatgcc gccaccttgg tggcgctgca atggctgcaa
     3241 ctgaaccatg cccgggtgcg tgcgcagtgg cagtgctgac cgcgacacca gcgccggtcg
     3301 ctggcaggcg gcgctaccgg gtcgatctgg tggcacagca ggcgcaggcg gccagcaact
     3361 accagcggct gctgaggctg ctgcccgagc tgcaccagcg gacgtaccgt gagctgctgc
     3421 tgcgggagcg gctggtccgc ttcgtcatca cccatccatc gcgttacacc agcttcgtca
     3481 ccattcacca tgaccagccg cgcccgccgt ggctggcgcc ctggtgcatg gagctgtcgc
     3541 tctaccacga tgctggcctt gccgaggtgg tgcgctgcat ggaccagcgg gcggtcgcgg
     3601 cggttcacga ctaccccaac cggcggatgc atcaggtcga cgagaagtgg caactgaact
     3661 tttttctggg tgagtggctg cggttctgtc tgcaacaggg gcgggccacc gcctgcgaac
     3721 tgccgttcct gccggcaggt tgagcgatgc gtgacacctt cgtcgagctg gtgcagatca
     3781 gcgacagcca cctctttgcc gaccgcgagg ccgagctgct gggcatcaac accgacgcca
     3841 gtctggctgc ggtgctgcaa cggctcgacg ggcgaccgcg gccggccctg ctgctggcca
     3901 ccggtgacaa cagccaggat ggcagcgtcg cggcctaccg ccgctttctg gaacaggtgc
     3961 agggtcgcgg cgagcgtttt gtctggctgc cgggcaacca cgacctgccg gacgtgatgg
     4021 cggcgctgtc gccggcgggc actctggtga agtcgctcga tctgggggcg tggcggctgc
     4081 tgctgctcga ctcgacgctg ccggcgacgg tggcgggcca tctggccgaa gcggagttcg
     4141 acttcgtcga gcggtcgttg tgcagcgcac cgcagcggcc gacactgctg gcggtgcacc
     4201 atccgccctg cgcgctcggc tgcgcctggc tcgatgacca gggcatcgac aacggcgcac
     4261 ggctgctggc gctgctggcc gaccatccgc aggtcaggct gctgctgtgt ggtcacatcc
     4321 atcagcagca ggcccggcag ttcggtccgc tctggctgct gtcggcgccc tcgacctgcg
     4381 tgcagttccg gcccgactcg gacgactttg cgatcgacag cctggcgccc ggcctgcgct
     4441 ggctgcgact gcatgacgat ggccggttcg agaccggcat cgaacgggtg gccgctggcc
     4501 tgttcaccgc cgaccacagc agttccggat attgatcacc gttctctttg ccacggccga
     4561 atccgatcat gagtgactac aacgccgccg ccatcgaagt gctgaccggg ctcgacccgg
     4621 tgcgcaagcg cccgggcatg tacaccgaca ccagccgccc caaccatctg gtgcaggagg
     4681 tggtcgacaa cagcgtcgac gaggcgctgg cgggccatgc gaccctgatc gagctgatcc
     4741 tgcatgccga tggctcgatc gaggtgaatg acgatgggcg cggcatgccg gtcgatctgc
     4801 acccggtcga gggggtgagc ggggtcgagc tgatcctgac ccggctccat gccggcggca
     4861 agttctccgg cgaccagtac cgcttctccg gcggtctgca tggcgtcggc gtttcggtgg
     4921 tcaatgcgct gtcgctctgg ctggaggtgg aggtgcggcg cgacggcaag ctgtggcgga
     4981 tgcgcttcga atcgggacag aaggtgaccg agctgaccga ggtcggcagc gcgccacggc
     5041 gggtcagcgg cacccgggtc cggtttctgc ccgatccggc cttcttcgat tcacccagac
     5101 tggccctgcc acggctcaag cagctgctga aggccaaggc ggtgctctgt cccggcctca
     5161 gactgcgctt cgaggagcgt gccgagggcg agcagatcga atggtgctat gccagcggtc
     5221 tggccgacta tctgctcgag agtgcccagg gcgcgaccgc cgtgccgcca cagcccttca
     5281 ccggccactt cgaagggcgc gacgagcagg tcgactgggc gatcttctgg ctgccggagg
     5341 ggggcgagcc gatcgccgag agctatgtca acctgattcc gacgccgcag ggcggcaccc
     5401 atgtcaacgg gctgcgcgcc ggcctgaccg aggcgctgcg cgagttctgc gaattccgca
     5461 acctgctgcc gcgcgggctc aagctgacca ccgatgacct gttcgaacgg gccagctatc
     5521 tgctctcggc gcggcttcgt gaaccgcagt tctcgggaca gaccaaggag cggctctcct
     5581 cgcgtgaagc ggtcgccttc atctccggtg tcggcaagga tgccttcagc ctctggctca
     5641 accagcatgt cgaggtcggc gagctgctgg ccgaactggc gatcagccag gcgcaacggc
     5701 ggctgaaggc cagccggcag gtggcgcgca agcgggtgac ccagggtccg gcactgccgg
     5761 gcaagctggc cgattgcagc agccaggaga tggggcgtgg cgagctgttt ctggtcgagg
     5821 gtgactcggc agggggctcg gcgaagcagg cgcgcgaccg ggagttccag gcggtgatgc
     5881 cgctgcgcgg caagattctc aacacctggg aggtcgacag cagcgagatt ctcgcctcgc
     5941 aggaggtgca tgacatcgcc gtggcgctcg gcatcgaccc cggctccgac gacctgagcg
     6001 gactgcgcta cggcaaggtc tgcatcctgg ccgatgccga ctccgatggt cgccacatcg
     6061 cctcgctgct gaccgtgctg ttcctgcgcc actttccggc gctggtgcgt gccggacacc
     6121 tccatgtggc gatgccgccg ctctaccgca tcgacctcgg caaggaggtc tactactccc
     6181 tcgatgaggc cgaaaagatg ggcattctcg accggctgag cgccgagaag cggcgcggca
     6241 agcccaatgt gcagcgcttc aaggggttgg gcgagatgaa tccgctgcag ttgcgcgaga
     6301 ccacgatggc accggccacc cggcggctgg tgcggctcgg tgtcgacggc gaacaggcgg
     6361 cacacgagat gctcgacatg ctgttcgcca agcggcgcgc gtcggaccgc cgccactggc
     6421 tcgagcggca tggtggcacc gcggagctgg tctgacgcgc cagtcagcct gctccgggcc
     6481 ggaggcgcag cagcaccagc agcgccagcg cggtgcagag cgcgccagcc atgaaggtgg
     6541 ccgccgggcc gacccactcc cagagcgcgc ccgcgatcag gctggcggcc agcagcgcga
     6601 tgccggtggt cagccggaac agcccgaagg cagtgccgcg cagtgcggcc ggcgccgtgt
     6661 cggccaccat cgccgacagt accccctcgg tcagtcccat gtgcatgccc cacagcagcg
     6721 cgccgccaaa cagcagccac cagccgtcgg ccagcgccag catggcatcg gcggccagca
     6781 gcagcaggca acccatcgcc accagccgcc gtcggccgat ccggtccgag agccgcccgg
     6841 ccggatagct ggagagcaca taggccaggt tcatcgccac catcaccgcc ggtgcccagg
     6901 cgatcggcag tccgaggctg ttgccgcgca gcagcagaaa ggcctcgctg aagcgggcca
     6961 gcgtcagcag cagcgcaaag ccggtcactg cccagaaggc ggcgggcagg gcacgccagc
     7021 gaccggtcgg tgccgtaccc ttcaccggtg gttctggcgc gctggcaggc tgtggcggct
     7081 ccttcaccgc cagcagcaac agcaccgcca gcagcgccgg caccacggcg acccacagca
     7141 ccagtctgac atcgtcggtg aagcaggcca gcagccccat cgccagcagc ggaccgagca
     7201 gggcgccgat gctgtcgagc gattgccgca gaccaaagcc agcgccgcgc agcgtggccg
     7261 gcgtcagatc ggtcagcagg gcgtcgcgtg gcgcgccgcg aatgcccttg ccgacgcggt
     7321 cgagcagccg ggcggtgaac accatgccga tcgagctcgc cagcggaaac agcggtttgg
     7381 tcaacgccga gagtccataa ccgagcagca ccagcggctt gcgtcgtccc agccagtcgc
     7441 tcaggccgcc cgagaccgcc ttgaccaggg cggcggtggc ctcggccaga ccttcgagca
     7501 gacccagcac catcggcgac gcgccgagcg tggtgacgat gaagagcggc aggatgctgt
     7561 ggatcagctc cgaagagagg tccatgcaga gactgaccag tcccagcagc cagacgccgc
     7621 gcggaatcgg cgcgacggtg ggggcagggg agtggtgcat cggcaggtca attctggctg
     7681 cgtggtccgg ccagccggct caggttgcgc agcaggcgcc ggatggccgc gaccggttcg
     7741 acatcctcgc gctgcgccgc cagccgcagc agcggctccg gcacctcgcc attgcggctc
     7801 agcgccagtg cggcatgaaa gcggtgcgct tcatcgccgt cgcccagcgc ggcctgaaac
     7861 agccggctgc tggcggcatc gagatcggcc ggacgaatcg ccagccacag cgagggtggc
     7921 aggctggcgc cggcatcgcg ccgctgttgc agccactcca ccactgccag gctcaggctc
     7981 gcattgagcc gcagctccag attctgcaga tcgagcaggt tggcgtcggc actcaatgcc
     8041 gccagcacca gctggttgaa ggcaggttga ggaaaatgca gcgccggata ggggttgtga
     8101 cggaacagcg ccgcctgtcg ctgggcaaag cgggccggca gtgccgcatc gatgctccat
     8161 gccgccaggc taccggtcgg gtcgaccagc atcagcatcc gcaggccact gccacgcacc
     8221 ggctccgaag ccgcctcgaa cagcttggtc aaccagcgcg cagggggtgc cgccgccagc
     8281 tccgcaccga gcagcagcaa cagcgcaatg gcgagatcgg cgcggcacca ctgccccagc
     8341 cgtatttcgg tcggccgggg ctcgcgcagg atcagcgaat agaccgcacc gggctcgctg
     8401 tcgcccagct cgcgccaggc cgcttcgcac agctccagtg agctggcgaa cagacgctgc
     8461 cgctccacct gctgcaacag ccgttcatga cgctccagcc agttcagttg cgccggcgtg
     8521 gcaaactcgc tgtaggcttg gcgcagcgtg cggtattgcg cttcattcag catgacgact
     8581 gctgttcgag gagttccgga tcgttcatcg gggtgttttc actgccaaaa gagatgggtg
     8641 ggtctgcgtg ttgccgagca gcggctagta taagagcgat tggcgaagag ggtttgaccg
     8701 atggcaactc gacactccag atgaatgcgc gtcttttcgg tgtgctgctt ctggcgctgg
     8761 gctgcggcct cagcctgtgg gcgcagggcg gggtcgatcg gacgacgctg gcgattctgg
     8821 tgaacgatgc cgacccgctc agcgtgcagg tggggcagta ttaccagcag gcgcgcaagc
     8881 tggaggccag acagttgatt cacctgaact ttccgaccgg aaagtcacgg ctctcgcccg
     8941 aggcctttgc ggccattcgc gcccagatcg agcgggccac gcccgacttc attcaggcct
     9001 atgccatcac ctggagccgt ccctaccggg tcggctgcat gtcgatcacc accgccgtgg
     9061 ccgccgggtt cgatgcggct ttctgtccgc ccgagggggt ggtctgcgcg ccgacccgcc
     9121 ggctcgacta cttcgacagc agcgtcaccg atccgcaccg gcaactgggt ctgcgtccgg
     9181 ccatgatgct ggccggcaac aatctggagg aggtgaagcg gctgatcgac cgcggcatcg
     9241 ccgccgatgg cagccatcct gccggcagtg cctacctgct ggtcaccgcc gaccgtgcgc
     9301 gcagcgtgcg tgccctcggc tatggcgagg tctttcgggt ggcacggcca ccgatccgcc
     9361 cgcgccgcat cgaggccgac tggatcgagc atgagcagga tgtgctcttc tacttcaccg
     9421 gcacggcctg ggtgcggcaa ctcgacagca accgctttct gcccggcgcg gtcggcgacc
     9481 acctgaccag cgccggcggc cagctcgatg gccagaagca gatgccggcc ctggcctggc
     9541 tgagcgccgg ggccaccggc agctacggca cggtgagcga accctgcaac tttcccgaaa
     9601 aattccccag gccggcggtg ctgatcgacc attaccagcg tggcgagacc ctgctcgaag
     9661 cctactggaa gagcgtggcc agcccggggc aggggctctt catcggcgag ccgctggccc
     9721 gcccctttgg tgaacgctga aaatgtgatc ggctgcgcac tctgtcgcgc tgtcgacacg
     9781 gagttcgtcg cccttttcta tactgctgca cagggctgtc cgttcctgcg tggccgcgtc
     9841 gagagtgggg aaagttgatg aaagcgcaaa gatttcgact ctggctgggc gggctgctgc
     9901 tcggtctggc gctgggttgt tcggctgatg ttttcaacca ctcctccaga acgctttttc
     9961 tgctcggcga agaggacggc aaggtgttcg aactgccacc gggtggtgca tttcttggtc
    10021 gtcaggatgc cattctgctg ccgagcggag caggggtacg ggtgttcaag accgtggacc
    10081 ggatcgatgt ggtggtgtcg ccacggcacg ccatcatcac ctatggcggc aatctgcttg
    10141 agcgttgcgg gcagtggctg atcggcggct ggctcgaccg tcgtccggtc ggccagcgct
    10201 gggttcagca gatcggccgg cactaccggg actctttcgg ctgaactggc ctgatatgtg
    10261 cctataaaac cccggccgca gcatggctgt gcagagtgtc atcaaactgc cagcattctg
    10321 ctgcagcatt cgcgaccgcc ctgccatggg gcagcgggct ggtatttctg gtgcctgatc
    10381 ctataatcgc accattcgag catgctgatg aagatgctcg aaaattcata taacccagcg
    10441 aatgaaaagg aagaactctc gtgacacaac aagtcggagc gcacaaggtg aaggtcggaa
    10501 aaaaattcga actcctcgcg aaggcgctgc ttgcagtggt gctgatgtcg acatcgaccc
    10561 tggtcctggc gctcgatccc tacctgatcc agccgggtga cgtgctggag atctcggtct
    10621 ggcgtgaaga tgggttgcaa cgcgaggcac tggtgcggcc cgatggcggc ctgagctttc
    10681 cgctggtggg cgagatcggt gccgccggcc agaccgtgcc ggcagtgagc gaagagatca
    10741 aaaaacggct caccaaattc attcccgacc cggtggtgac ggtggcgctc aagcagaacc
    10801 tcggcaacaa ggtctatgtc atcggcaagg tcaaccggcc gggcgaattt gccgtcaacc
    10861 gtgccgttga cgtgttgcag gcgctcagca tggccggcgg catgaacccc tttgccgaca
    10921 ccaagaacat ccaggtgctg cggcgggtca atggcgagca gaccagcgtc ggcttcaact
    10981 acgccgagat ggagcagggc aagaagttgc agcagaacat cctgctgcag gccggtgatg
    11041 tggtcgtggt gccatgaggc gggccacatt ttcgaccctg ctgacggcgg gggccttgct
    11101 ggcggcgggg tccgccacgg cgggtggctg gtatcaggag cccagcgtgc ggttgcgcac
    11161 cgggttcgat gacaacatcc ggctgcgcag cagcgatccg caggaagatt tcgtcaccac
    11221 gctgcaaggc aatggcctgt tcggttaccg caccgaaacc gaaagtgccg cactgaagct
    11281 gaaggccgag gccaaccagt atcagcagca ggatggtttg aacaacgaaa acctgtttgc
    11341 cgatctcaac ctcgatcaca agggcgaacg caacagtttc gggctgctga ccagcctgca
    11401 ccgtgacacc accctgcaga gcgaactgga gaccaccggg cgggtcgatg acaatctgcg
    11461 ggtgacgcgg caactgttgc agcccagttt cgagcacagc tttgctgaac gctgggtcgg
    11521 gtcggttggc gccagctact ccgaagtcga atatgaagac cgcgtgggca gcagtctgac
    11581 cgattaccgc tatgtcacgg cgcagaccgc gctgcaatac cgggtcagcg aagaccgctc
    11641 cttcaccggt acactctttg ccacccgcta cacccccacc gatttcgact tcatctcgcg
    11701 tgggcagggc gtgagcttcg gttggcagga gcgtttctcg cccagtctca ccggttcgct
    11761 ctcggtcgga gcgcgcaagg acaaatcgga gcggaccctt ttcggtctcc agaccgagga
    11821 tgaaagctct ggcacccagt tcgatgcggc gctggagaaa tcctggcagc gctggcggtt
    11881 gaatttaaac ttggggcgtt cgacggatcc aagtggcgac ggcagtctga ttcagcgtga
    11941 ccgcctgggc ggtacggcca gttatgactg gaacgagcgg acgcgattga tcttcaatgc
    12001 caactacctc gacattcagc aaaccaatga ccttgtttcg ctgagcagca atgaccgtcg
    12061 ctacctcacc ttcgcacccg gcatcgagtg gcgggtggag cgcaactgga ccgcacggtt
    12121 cgactaccgc taccggcaga acagatttct cgaccagggt gacgaggccg agagcaacgc
    12181 actctttgtt tcgatctcct atctctggcc aagcccactg ggcagcagcg ggcagggcaa
    12241 gccgctctga accggaatga ggctgaccgg gtgatcgcaa caccgcaatg aacatcgaag
    12301 acaacgctta ccgaaccgag gcaaaccctg catggaacag atgattggaa tcagtgacta
    12361 tctgcgcatc ctgcgccggc gcagggtttt gttgtggtcg gtgttcggcg tggtgttcgt
    12421 catcgcggtg ctggtgaccc tggccttgcc atcggtctac acctcgtcgg gcaccattct
    12481 gatcgagcag caggagattc cggccgagat ggtcaagtcg ac
//
LOCUS       DAORCQ010000068        12272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3410_length_12272_cov_6.045756, whole genome shotgun sequence.
ACCESSION   DAORCQ010000068 DAORCQ010000000
VERSION     DAORCQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_12272_cov_6.045756"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKI23_08065"
     CDS             <1..683
                     /locus_tag="PKI23_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36932.1"
                     /translation="PNRRPSNRRGRSSNNQRRADTAALGNEVAMPTAASTDSAVEQYP
                     QQQPEPAMTAPIERDAAAEVRIEPASRESAPAPVMLTMEPAVSVPAEVEAPSAAPSTE
                     TPTSAPMPTAPRAEVPAESPAPRSTEADDRSESIISRAGAFERGGFRVPNDPRIAPRK
                     VALEAVESRPSQRPTAQEQEMVQEPMATVTPTVEKIARAPNDPRARRTTPSSASSDEE
                     RPAAPEPQ"
     gene            961..1326
                     /locus_tag="PKI23_08070"
     CDS             961..1326
                     /locus_tag="PKI23_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN36933.1"
                     /translation="MSVPLLICDDSAMARKQVVKALPAEWDVDITLATNGVEALDAIR
                     QGRGEVVLLDLTMPEIDGYGVLERVRSEGLKAAIIVISADIQPEARDRVMKFGALDFI
                     KKPVDHHRLHDALRKCGLI"
     gene            1323..1958
                     /locus_tag="PKI23_08075"
     CDS             1323..1958
                     /locus_tag="PKI23_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36934.1"
                     /translation="MNRTLALSEDQRDCLQEVVNVAMGQAGDSLARFLEVFIQLSVPR
                     IQLLHRDQLIHQLTTMTGGDRVRVSGVSQGFYQLSGSRGMRGEAIVLFTDSSFRELAD
                     LLAYDSTLSMETEKELLLDVANILSGACLNGIAEQFDTEMGFSTPAILGQRISVHAML
                     EHEKPEWDHALLVEISYTLEDRSFGCTLFLLMPGESILLVKRMLDQLLELL"
     gene            1959..2921
                     /locus_tag="PKI23_08080"
     CDS             1959..2921
                     /locus_tag="PKI23_08080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNN36935.1"
                     /translation="MSAFSPSSQLLECVVDRINVGVFMVDIDLEIQFWNGFMATHSGI
                     RADEVLGRKLFDCFPQLPQRWLEKKINSVFMLKNFAFSSWQQRPYLFQFRHNRPVTGD
                     MEFMRQDLTLIPIKRDSGEVDHVCIILLDVTDTAIYHALHQSAMQKLENASRIDGLTQ
                     LLNRSHWQSRLTEEFNRATRYQQPLSLILFDLDHFKSINDSHGHLGGDAVLVEVAAIV
                     KKSLRDSDIAGRYGGEEFGLILPGTPAAEAMRTAERLRTTLQEQPILFEGTPIAVTAS
                     LGIGALDATIADENGLIAAADAALYSAKRSGRNRAVLHEERQSA"
     gene            complement(2952..3986)
                     /locus_tag="PKI23_08085"
     CDS             complement(2952..3986)
                     /locus_tag="PKI23_08085"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNN36936.1"
                     /translation="MKTARRVTVLGGGSFGTVLANILAENGHTVWLWMRDRDRVEQVA
                     QSRENAAYLPGYRLHDALQVTAEMAACLPDSEIVFVSVPSQTVRAVVRAASAWLPSDV
                     IMVSTAKGIEAEGFRMMSQVLVEEMPNARIGALSGPNLAREIAARFPTATVIASADEA
                     LCNTIQETLGCDYLRVYSAIDLYGVQLAGALKNIYAIITGMADALGMGANTRGMLITR
                     SLAEMSRFAVRLGAEPMTFLGLTGVGDLVVTCTSPLSRNYRVGALVGRGKSVAEAMAE
                     VGQVAEGVNTLNLVKQKAIELDIYMPLVMGLDAVLSNPRMAFDVAKALMLGSGKRDVE
                     FSLRGQQQVV"
     gene            4204..4515
                     /locus_tag="PKI23_08090"
     CDS             4204..4515
                     /locus_tag="PKI23_08090"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNN36937.1"
                     /translation="MFVAMNRFRIARGQEDAFTEIWQTRDSHLQGVPGFREFHLMRGP
                     TEEDHTLFASHSVWESRQDFENWTRSEAFRKAHANAGNASGVRTMYLGPPKLEVFESV
                     L"
     gene            4664..5182
                     /locus_tag="PKI23_08095"
     CDS             4664..5182
                     /locus_tag="PKI23_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNN36938.1"
                     /translation="MSDSNTPILTPELLAWIGKEGPVSTAEVRLEAIQNYANAVGFGG
                     PDPLYFDEEFAQGTPYGGITAPPMFFGTPFSRIVPHMDLRDDGLPKDDDVHSGLRPPL
                     PLPRLMGGGTEVEFFRPVRPGDRLTRRSKLADLTEKNGRTGPLVFTTIETEYRNEADE
                     LVLIVRSSTISR"
     gene            5203..5688
                     /locus_tag="PKI23_08100"
     CDS             5203..5688
                     /locus_tag="PKI23_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36939.1"
                     /translation="MNSIPAPTTRRLMWPYVTEGDLLPPLSVTIGAAQLFQLSAVMNL
                     AHRIHYDLEWARHEGYPNLVIHAPFHGEVMVQMIRNWMGPSGWLKKISYQNRHYAVLG
                     DTLTGSGVIARRYEEGDLFLVDLKLEVSKTGPDGERIITAPGTATVALPTRAVQVPIP
                     H"
     gene            complement(5766..6605)
                     /locus_tag="PKI23_08105"
     CDS             complement(5766..6605)
                     /locus_tag="PKI23_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNN36940.1"
                     /translation="MTDLCAQVRSDILQAIEADRLVMPTLPEVALTIRTEASNPNSTA
                     ASLARAIGRDAALSARLLKIANSPLLRASRAINDLQMAISRLGVQYSSNLATGLAMQQ
                     MFQSSSRAIDQRLRQIWHESTEVAGIALVLTQNFTRLKPDQAMLAGLMHRLGALPILT
                     YAEGRSELLASPALIDRLIEELHHELGRAILERWEFPPALVEVPERYRDFGRDSSAPD
                     YVDVVMVAALHSRLDSDHPDLKLDWSTIPAFGKLGLDPDIDARQIEDVSDEMEAAMAL
                     LNG"
     gene            complement(6651..7673)
                     /locus_tag="PKI23_08110"
     CDS             complement(6651..7673)
                     /locus_tag="PKI23_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNN36941.1"
                     /translation="MSRWQEFLRSQGARFDGFELLDFGDPQAGQGASTLLTGLSHEGV
                     IQVSGVESEKFLQGQLTCDLREVDSHHARLGALCTYQGRAVATFILNRIGSDYHLQLD
                     RSALPQLLATLGKYIVFSKATLRDASDEWVRIGLAGHHASELLGANFGALPAADYEVM
                     PLSAGAILRLPGSVARFQIWLPFDAALDIWPRLKQGATLVGSDGWRLLEIRAGVPMVT
                     AATQEAFIPQMLNFDELGGINYRKGCYTGQEIIARSHYRGKQKRHTQRAVVADAVERI
                     APGSEIHAAGEAAPVGTVVTAARAGAGWELLAVLKDDRLDAELRWGDSNGPPVRLLDL
                     PYTLSL"
     gene            7779..8045
                     /locus_tag="PKI23_08115"
     CDS             7779..8045
                     /locus_tag="PKI23_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNN36942.1"
                     /translation="MVDETELHRTYWHSRRGMLELDLILLPFVKMHYPNLGPADRASY
                     QRLLREEDQSLFLWLLGRETAPDAELARMIGKIRAATTAPDHPA"
     gene            8053..8502
                     /locus_tag="PKI23_08120"
     CDS             8053..8502
                     /locus_tag="PKI23_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36943.1"
                     /translation="MLLIALVGLAHGAGLIGLLSAELPATVRWGGTTLLLLSCALQES
                     RLRRRHPDRIVRLTLHPHHTELTTYGGQRRLATLEPGTRITPWCIALSLRPFESPWWR
                     TRYPLLLLPDALTATDWRRLRIGLQQGHGRRRTADDGDQHGVERTGQ"
     gene            complement(8416..10056)
                     /gene="nadB"
                     /locus_tag="PKI23_08125"
     CDS             complement(8416..10056)
                     /gene="nadB"
                     /locus_tag="PKI23_08125"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNN36944.1"
                     /translation="MPQPASYDLLIIGAGAAGLTLALKLAAHLKIALVSKGSLIESST
                     YFAQGGIAAVLAENDSIEAHVADTLRAGAGLCHRDAVEFTVRNSRGIIEWLIEQGVPF
                     TKTDRPEQGFEDYHLTQEGGHSLRRIIHAEDATGQAVSNALIDRLHSHANIGVFEYRT
                     AVDLIVQQDAQGERRCTGAYLLNQQSGEVEVFQARFTALATGGASKVYLYTSNPDTAT
                     GDGIAMAWRAGCRVANMEFIQFHPTCLYHPKAKSFLISEALRGEGARLLLPDGTAFMQ
                     RFHPQRELAPRDVVARAIDHEMKRLGIDHVYLDISHQPADFIRSHFPTIYGRCLEFGI
                     DITREPIPVVPAAHYTCGGVVVDLDGATDLPGLYAIGESSCTGLHGANRMASNSLLEC
                     LVYAQAAAQSILRRSDATPVPAPTRPWDESRVIDSDEEIVIAHNWEELRRFMWDYVGI
                     VRTDKRLARALRRVELLKQEIADYYGHYKVTSDLLELRNLACVAELIVRCAIARKESR
                     GLHYTLDYPDLLPRALDTVLIPVIGGAAPTMTLLEPNP"
     gene            10316..10891
                     /gene="rpoE"
                     /locus_tag="PKI23_08130"
     CDS             10316..10891
                     /gene="rpoE"
                     /locus_tag="PKI23_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNN36945.1"
                     /translation="MTQSDNDQQLVARVQKGDKRAFDLLVLKYQHKIVSLVSRYVHDN
                     HEAEDVAQESFIRAYRALRDFRSESAFYTWLYRIAINTAKNHLATRHRRPPNSDIEIE
                     DAELQGGHNQLRETASPEGLLMRDELEQVVNRTIRELPEELRAAITLREYDGLSYEEI
                     AEIMECPVGTVRSRIFRAREAIDQRIAAMVQ"
     gene            11061..11627
                     /locus_tag="PKI23_08135"
     CDS             11061..11627
                     /locus_tag="PKI23_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RseA family anti-sigma factor"
                     /protein_id="HNN36946.1"
                     /translation="MNEAIEESLSALLDGEADELETMRVLRALETDPELREKWRRYHM
                     MVTLLRGEQKVSSRLDIGRKVTLSLAGESVAQRNRSEQKTRPWSKSVAILAVAASTTF
                     IVVFGAQQWGRIDNSQNPATLAAQNATPTTSASTAPTHLQPTTTETALATDQERLERH
                     MSRHAQGRLGSEPTLTQPFARPASLDQQ"
     gene            11624..>12272
                     /locus_tag="PKI23_08140"
     CDS             11624..>12272
                     /locus_tag="PKI23_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HNN36947.1"
                     /translation="MIPSSLRWVLLRGWPLLLPLCLASSALWAESADEWLLRMGQAFR
                     EQNYDGTFIYVRGDELQSFRIIHRADAGIEQERLIHLDGAAHELLRTGEGLFAIQADA
                     PISETAAKASFAQSFAREPGQLAQHYRLELLGDERVSNRQCRRIAIRPHDPDRYSHEL
                     WIDQASGLLLRSLLRDTDGRTLERFQFVTLRVADDVSVGDLTSMTHGSTTLHRVHP"
BASE COUNT         2383 a         4120 c         3657 g         2112 t
ORIGIN      
        1 cacccaaccg ccgccccagc aaccgccgtg gccgcagcag caacaaccag cgccgggccg
       61 acacggcggc actcggcaat gaggtcgcca tgccaaccgc tgcctcgact gactccgcag
      121 tcgagcaata cccgcagcag caaccggaac ctgccatgac ggcgccgatc gagcgcgacg
      181 ctgctgccga ggtcagaatc gaacctgcct cacgggagtc tgcccccgct ccggtgatgc
      241 tgaccatgga gcctgcggta tcagtgcccg ccgaggtcga agccccttcg gctgcaccat
      301 ccaccgagac gccgacgtct gcacccatgc cgacagcgcc cagagctgaa gtgcccgccg
      361 aatcgcctgc tcccagatcg accgaagccg acgaccgctc cgaatcgatc atcagccggg
      421 ccggtgcctt cgagcgcggc gggttcaggg tgcccaatga cccgcgcatc gcaccgcgca
      481 aggtcgcgct ggaggcggtg gagagcaggc cgagccagcg accaacagcg caggagcagg
      541 agatggtcca ggagccgatg gcaacggtca ctcccacggt cgagaagatc gcgcgcgcgc
      601 ccaatgaccc gcgcgcacga cgcaccactc catccagcgc atcatccgac gaagagcgcc
      661 ctgcggcacc agagccgcaa tagcgcggcc ggacgctcag ggcagacgct cttctgccct
      721 gagcgtggcg cccagacgcc cggttttgcc atttcccaca gcccgagcct cgccatcagg
      781 ctccctgcga cctctcggcc cgcccttttc cgcgtcgcac tgccaaagcc tcccggcagg
      841 acaacgacac accgcgcaca atcggtgcgg caaaactctt gctttccatg tggtctgaaa
      901 aatcggaact atacttccga tcaacctatg ttcactcagg cgtgtgctcc agagaacaac
      961 atgtccgtac ccttactgat ttgcgatgac tcggcgatgg cccgcaagca ggtggtcaag
     1021 gcgctgcctg ccgagtggga tgtcgacatc accctggcca ccaatggggt cgaagcgctc
     1081 gatgccattc gccaaggcag aggcgaagtg gtgttgctgg atctgacgat gcccgaaatc
     1141 gatggctatg gcgtgctgga aagggtcaga tcagaaggac tcaaagccgc catcatcgtg
     1201 atttcggccg acattcagcc cgaggcccgc gatcgggtga tgaagttcgg cgcgctcgat
     1261 ttcatcaaaa aaccggtgga ccaccacaga ttgcacgatg cgctgcggaa gtgtggcctg
     1321 atatgaacag gacgctggcg ctgtcggaag atcaacggga ctgcctgcag gaggtggtca
     1381 acgtcgccat gggacaggcc ggcgactcgc tggcccgctt tctggaggtc ttcatccagc
     1441 tctcggtgcc gcgcatccaa ctgctgcatc gggaccagct catccaccag ttgacaacca
     1501 tgaccggggg cgatcgggtc agggtctccg gcgtcagcca aggcttttac cagttgagcg
     1561 gcagccgtgg catgcgcggc gaagccatcg tgctctttac cgacagctcc ttcagggagc
     1621 tggccgatct gctggcttac gacagcactt tgagcatgga gaccgagaaa gagctgctgc
     1681 tcgatgtcgc caacattctc agcggtgcct gcctgaatgg catcgccgaa cagttcgaca
     1741 ccgagatggg tttctccacg ccagccattc tgggtcagcg catctccgtc cacgcaatgc
     1801 tggagcacga aaaaccagag tgggatcacg cattgctggt tgaaatctcc tacacccttg
     1861 aagaccgctc attcggttgc actctgttcc tgctgatgcc gggtgaatcg atcctgctgg
     1921 tcaagaggat gctcgaccag ctgctggaac tgctctgagt gtccgctttc tcgccctcct
     1981 cgcaactgct ggagtgcgtc gtcgaccgca tcaatgtcgg cgtgttcatg gtcgacattg
     2041 atcttgaaat tcagttctgg aacggattca tggccaccca cagcggcatc cgtgccgatg
     2101 aggttctggg cagaaagctg ttcgactgct ttccgcaact gccgcagcgc tggcttgaaa
     2161 agaagatcaa cagcgtgttc atgctgaaaa atttcgcctt cagctcatgg cagcagcgcc
     2221 cctacctgtt ccagttccgg cacaaccgcc cagtgaccgg tgacatggag ttcatgcgtc
     2281 aggatctgac gctgataccg atcaagcgtg acagtggcga agtcgatcat gtctgcatca
     2341 tcctgctcga cgtgaccgac accgcgatct atcacgccct gcaccagtca gcgatgcaga
     2401 aactggaaaa cgccagccgc atcgatggcc tgacccaact gctcaatcgc agccactggc
     2461 aatcccgtct gaccgaggag ttcaaccggg caacacgtta ccagcaaccg ctgtcgctga
     2521 tcctgttcga cctcgatcat ttcaagtcga tcaacgacag ccatggccat ctgggtggcg
     2581 acgcggtgct ggttgaggtc gcggccatcg tcaaaaagag cctgcgcgac tcggacatcg
     2641 ccgggcgcta cggcggcgag gagttcggcc tgatcctgcc aggcaccccg gcggccgagg
     2701 cgatgaggac cgcggagcgc ctgcgcacca ccctccagga gcagccgatc ctgtttgagg
     2761 gaacgccgat tgcggtgacc gccagcctcg gcatcggtgc gctcgatgcc accattgccg
     2821 atgagaacgg gctgatcgcc gcggctgacg ccgcgctcta cagcgccaag agatcgggtc
     2881 gcaaccgtgc tgtgctccac gaggagaggc aaagcgcgta aagcaccccg cagggcgcgt
     2941 ccgcccacgg atcagaccac ctgctgctga cccctcaggg agaactccac gtcacgcttg
     3001 ccgctgccga gcatcagcgc cttggcgaca tcgaaggcca tgcggggatt gctgagcacc
     3061 gcatccagtc ccatcaccaa cggcatgtag atgtccagtt cgatcgcctt ctgcttgacc
     3121 agattcagcg tgttgacccc ttcggccacc tgcccgacct cggccatggc ctcggccacg
     3181 ctcttgccac gccccaccag ggcaccgaca cggtagttgc ggctgagcgg tgaggtgcag
     3241 gtcaccacca ggtcgcccac accggtcagg ccgagaaagg tcatcggctc ggccccaagg
     3301 cgtaccgcaa agcggctcat ctcggccaga ctgcgggtga tcagcatgcc gcgggtgttg
     3361 gcgcccattc ccagcgcatc cgccatgccg gtgatgatcg cgtagatgtt cttcagcgcg
     3421 ccggccagct gcacgccata caggtcgatg gcggaataga cgcggaggta gtcgcagccc
     3481 agggtctcct gaatggtgtt gcagagcgct tcatccgcgc tggcgatcac cgtggccgtt
     3541 ggaaaacgcg cggcaatctc ccgcgccaga ttgggcccgc tcagggcgcc gatgcgggcg
     3601 ttcggcatct cttcgaccag cacctgactc atcatgcgaa aaccttccgc ctcgatgccc
     3661 ttggcggtgc tgaccatgat gacatcgctc ggcagccagg cactggcggc acgcaccacc
     3721 gccctgaccg tctggctcgg cacactgaca aagacgatct cgctgtcggg cagacaggcg
     3781 gccatctctg ccgtcacctg aagcgcatca tgcagccgat agcccggcag gtaggcggca
     3841 ttctcgcgcg actgcgcgac ctgctcgaca cggtcacggt cgcgcatcca cagccagacg
     3901 gtgtggccat tctcggccag gatgttggcc agtaccgtgc cgaaggagcc tcctcccagc
     3961 accgtgacgc gcctggcggt tttcatctca tcttttattg tgcttgtcat gccggcgaat
     4021 ccaaccaggg ccaccctgtg ccatgaggcg caaagcggcg gcatcataac ccaggcggct
     4081 ccgcgtccca aacagacgcc cgtgctcacc cgaagcccaa tctcaccatg ggcaaattgt
     4141 cttttcaaac cgccttgcta agatgccgct gttcaaagat caataccgac aggagaattc
     4201 agcatgtttg tcgccatgaa caggttccgg atcgcgcgcg gccaggaaga tgccttcacc
     4261 gaaatctggc agacccgtga cagccacctg cagggtgttc ccggcttcag ggagtttcat
     4321 ctgatgcgcg gaccgaccga agaggaccac accctgttcg cctcccactc ggtctgggag
     4381 tcgcgtcagg atttcgagaa ctggacccgc tccgaagcct tccgcaaggc ccacgccaat
     4441 gccggcaacg ccagcggcgt gcgcaccatg tatctggggc cacccaagct cgaagtgttc
     4501 gagtcggtac tctgaaccga ctcgaccgct acaggccggc gtggtcaatc gcgccgcgca
     4561 aggcagcaga ttgtctgcca gtgcaatttg tgcttcgata ccgctggcat cggtcatcga
     4621 ccgaacaccc ataacaataa agtcaggatg gcaggatgac accatgagtg acagcaacac
     4681 tccgattctc acccccgaac tcctcgcctg gatcggcaag gagggccccg tctcgacggc
     4741 cgaagtcagg ctggaggcaa tccagaacta cgccaatgcg gtcggcttcg gcggacccga
     4801 tccactctac ttcgacgagg agttcgccca gggcactccc tatggcggca tcaccgcgcc
     4861 gccgatgttc ttcggcacgc cgttcagccg catcgtcccg cacatggatc tgcgcgacga
     4921 tggcctgccg aaggatgacg acgtgcacag cggtctgcgc ccgccgctgc cactgccccg
     4981 attgatgggc ggcggcaccg aggtggagtt cttccgcccg gtccgtcccg gcgaccgtct
     5041 gacccggcgc tcgaagctgg ccgatctgac cgaaaaaaat ggccgcaccg gcccactggt
     5101 cttcaccacc atcgagaccg agtaccgcaa cgaggcggac gagttggtgc tgatcgtcag
     5161 aagctcgacc atttcccgct gagcggcggc aaggagaacg tcatgaattc gattcccgca
     5221 cccaccaccc gccggctgat gtggccctat gtcaccgaag gcgacctgct gccaccgctg
     5281 tcggtcacca tcggtgccgc ccaactgttt caactgtcgg cggtgatgaa tctggcccat
     5341 cgcatccatt acgacctcga atgggcccgt cacgaaggct accccaatct ggtcatccat
     5401 gcgcccttcc atggcgaggt gatggtgcag atgatccgca actggatggg accctccggc
     5461 tggctgaaga agatctccta ccagaaccgc cactatgcgg tgctcggcga cacactgacc
     5521 ggcagcggcg tcattgcccg tcgctatgag gagggcgatc tgtttctggt cgatctgaag
     5581 ctggaggtga gcaaaaccgg cccggatggc gagcgcatca tcaccgcacc cggcaccgca
     5641 acggtggccc tgccaacccg tgccgtgcag gtgccgattc cgcattgacc tgcaaccgcc
     5701 cgcagtggtg cccgatcagt tccagactgt cggcgtcacc atggcacgca tcggcgctgc
     5761 ggctttcagc cgttcagcag cgccatcgcc gcttccatct cgtcgctgac atcctcgatc
     5821 tgccgggcgt cgatgtcggg atcgagcccc agcttgccga aggccggaat ggtgctccag
     5881 tcaagcttga gatcgggatg gtcgctgtca agacggctgt gcagtgccgc aaccatcacg
     5941 acatcgacat agtccggcgc actgctgtcg cgaccaaaat cgcggtagcg ttccggcact
     6001 tcgaccagtg ccggcggaaa ctcccagcgc tcgagaatgg cgcggcccaa ctcgtggtgc
     6061 agctcctcga tcaaccgatc gatcaacgcc ggtgacgcca gcagttcaga ccggccttcg
     6121 gcataggtga gaatcggcag tgcgcccagg cgatgcatca acccggccag catggcctga
     6181 tcaggcttga gcctggtgaa attctgcgtc agcaccagcg cgatgcccgc cacctcggtg
     6241 ctctcgtgcc agatctgccg cagccgctga tcgatcgccc tggagctgct ctggaacatc
     6301 tgctgcatcg ccaacccggt ggccaggttg ctgctgtact gtacccccag ccggctgatt
     6361 gccatctgca gatcattgat ggcgcgcgaa gcgcgcaaca gcggactgtt ggcgatcttc
     6421 agcaaccggg cgctcagcgc ggcatcgcgg ccaatggccc gtgccaggct ggcggcggtg
     6481 gagttgggat tgctcgcttc ggtccggatc gtcaatgcca cctccggcag ggtcggcatc
     6541 accagccgat ctgcctcgat ggcttgcaga atgtcgctgc gaacttgtgc gcagaggtcg
     6601 gtcatgtcat tgctgcctgc tcaaaagaag aaacggcagc ccacggccgc ctacagcgat
     6661 aaagtatagg ggagatcgag cagtctgacc ggcggcccgt tgctgtcgcc ccagcgcagc
     6721 tcggcatcca gccggtcatc cttgagcacc gccagcagct cccagcccgc accagcgcgc
     6781 gctgccgtca ccaccgtgcc gaccggagcg gcctccccgg ctgcatggat ttcgctgcct
     6841 ggcgcgatcc gctccaccgc gtcggccacc actgcacgct gggtgtgccg cttctgcttg
     6901 ccacggtagt gcgagcgggc aatgatctcc tgaccggtgt agcagccctt gcggtagttg
     6961 atgcccccca gctcgtcgaa attgagcatc tgcggaatga aggcctcctg ggtcgccgcc
     7021 gtcaccatcg gtacgccggc acgaatttcc agcaaccgcc atccatcgct gccgaccagg
     7081 gtggcgccct gcttcagccg tggccagatg tcgagggcgg catcgaacgg gagccagatc
     7141 tgaaaacggg ccacactgcc cggcagacgg aggatcgccc ccgcgctcag cggcatcacc
     7201 tcatagtcag cggccggcaa cgcaccaaag ttggcgccga gcagttcgct ggcatggtgg
     7261 cccgccaggc cgatgcgcac ccactcatca ctggcatcac gcagcgtggc cttggagaag
     7321 acgatgtact tgcccagtgt cgccaacagc tgcggcagcg ccgagcggtc gagctgcagg
     7381 tggtagtcgc tgccgatgcg gttgagaatg aaggtggcca ctgcgcgccc ctgataggtg
     7441 cagagcgcgc cgagccgcgc atggtggctg tccacttcgc gcagatcgca ggtcaactgc
     7501 ccctgcagaa acttttcgct ctccacaccg ctgacctgaa tcaccccctc gtggctgagg
     7561 ccggtcagca gggtcgatgc gccctgtcca gcctggggat cgccaaagtc gagcagctcg
     7621 aacccgtcga agcgggctcc ctggctgcgc agaaactcct gccatctgga catacactac
     7681 tccctgccaa acagtggtga acaatcggca gcccccttga cgggtcgacc gatcgtggcc
     7741 gctataatag ccgctgcaac aggcgcagag gcaatcagat ggtggatgag acggaactgc
     7801 atcggacata ctggcacagc cgacgcggca tgctggagct ggacctgatt ctgctcccct
     7861 tcgtcaagat gcactacccc aacctcggcc cggccgaccg cgccagctat cagcggctgc
     7921 tgcgcgaaga ggaccagtcg ctctttctct ggctgctggg ccgcgaaaca gcgcccgatg
     7981 ccgaactggc gcggatgatc ggaaaaatcc gtgcagcaac caccgcccct gaccatccgg
     8041 cttgagcagt cgttgctgct cattgcactg gtcggactgg cacatggcgc cggcctgatc
     8101 ggactgctga gcgccgagct gcccgctacc gtgcgctggg gcggcacgac actgctgctg
     8161 ctgtcatgcg cactgcagga atcgcggctg cgtcgacgcc atcccgaccg catcgttcga
     8221 ctgacgctgc atccgcacca caccgagctg accacctacg gcggacagcg ccgactggcc
     8281 acgctggagc ccggcacgcg catcaccccc tggtgcattg cgctcagcct ccgtccgttc
     8341 gagtcaccct ggtggaggac acgctacccg ctgttgctgc tgcccgacgc cttgacggcc
     8401 accgactggc gccgattacg gattgggctc cagcagggtc atggtcggcg ccgcaccgcc
     8461 gatgacgggg atcagcacgg tgtcgagcgc acggggcaat agatcaggat aatcaagggt
     8521 gtagtgcagc ccgcggctct ccttgcgggc aatcgcgcag cgcacgatca actcggcgac
     8581 acaggcgagg ttgcgcagct ccagcagatc gctggtcacc ttgtagtggc cgtagtagtc
     8641 ggcaatctcc tgcttcagca gctcgacccg gcgcaatgcc cgcgccagcc gcttgtcggt
     8701 gcgcacgatg ccgacatagt cccacatgaa tcggcgcagc tcctcccagt tgtgcgcgat
     8761 cacgatctcc tcgtccgaat cgatcaccct ggattcatcc cacggccggg tcggcgccgg
     8821 caccggtgtc gcgtcactgc ggcgcagaat cgactgggcc gccgcctggg catagaccag
     8881 acattcgagc agcgagttgc tggccatgcg gttggcacca tgcagtccgg tgcaactgct
     8941 ctcgccgatg gcgtagagtc cgggcaggtc ggtggcacca tcgagatcga ccaccacccc
     9001 gccacaggtg tagtgggccg ccggcaccac cggaatcggc tcgcgggtga tgtcgatgcc
     9061 gaactccagg cagcgaccgt agatggtcgg aaagtggctg cggatgaagt cggccggctg
     9121 gtggctgatg tcgaggtaga catggtcgat gcccagccgc ttcatctcgt ggtcgatggc
     9181 gcgggccacc acatcacgcg gtgccagctc ccgctgtgga tgaaaccgct gcatgaatgc
     9241 ggtgccatcg ggcagcaaca gtctggcccc ctcgccacgc agcgcctcgg aaatcagaaa
     9301 ggatttggcc ttggggtggt agaggcaggt gggatggaac tggatgaact ccatgttggc
     9361 cacccggcag ccggcgcgcc aggccatggc gatgccatca ccggtggcgg tgtcggggtt
     9421 ggaggtgtag agatagacct tgctcgcccc gccggtggcc agcgcagtga agcgcgcctg
     9481 aaacacctcc acctcgccgc tctgctggtt gaggagataa gcaccggtgc agcgccgctc
     9541 tccctgcgca tcctgctgca cgatcagatc gaccgcggtg cggtactcga acacaccgat
     9601 gttggcgtgg ctgtgcagcc ggtcgatcaa cgcattggag accgcctgcc cggtcgcatc
     9661 ctcggcatgg atgatgcggc gcagactgtg ccccccctcc tgggtcaggt ggtaatcctc
     9721 aaaaccctgc tcgggccggt cggtcttggt gaagggcacc ccctgctcga tcagccactc
     9781 gatgatgccg cggctgttgc gcaccgtgaa ctcgaccgca tcgcggtgac acagcccggc
     9841 accggcacgc aaggtgtcgg ccacatgcgc ctcgatgctg tcattctcgg ccagcaccgc
     9901 ggcaatgccg ccctgcgcga agtaggtcga actctcgatc agactgccct tgctgaccag
     9961 cgcgatcttc aggtgcgcgg ccagtttgag cgccagggtg agaccggcag cgcccgcgcc
    10021 gatgatgagc aggtcgtagg aggcaggttg cggcatgaac agaagggtcc ggattgatca
    10081 ccataaggtg acagagtgat gaagcggcca atttaaccag aaatggaccc gggccggcag
    10141 tctctcttta ttccatcgca tggctgcatc gggcgaattt ctgctaggat ggcgcccttt
    10201 ggcccatccc acggcgaatg agatgcttca cttgcgtttc cgatcaggaa catgcccacc
    10261 tccaaaccac gcctgatcgg gcagccgcgg ctgtcccaac tccactgaac cgaacatgac
    10321 ccagtcagac aacgaccagc agctggtcgc gcgggtccag aaaggggaca aacgcgcttt
    10381 cgacctgctg gtgctcaagt accaacacaa gatcgtctcg ctggtcagtc gctatgtgca
    10441 tgacaaccac gaggcggaag atgtggcgca ggagtccttc atccgcgcct accgtgcact
    10501 gcgcgacttc cgcagcgaga gcgcctttta cacctggctc taccgcatcg ccatcaacac
    10561 cgccaagaac cacctggcca cgcgccaccg ccgtccgccc aacagcgaca tcgagatcga
    10621 ggatgccgaa ctgcaagggg gacacaacca gctcagggag acggcctcgc ccgaagggct
    10681 gctgatgcgc gatgaactcg agcaggtcgt caaccgcacc atccgcgaac tgcccgaaga
    10741 gctgcgtgcg gccatcaccc tgcgcgagta tgacggcctc agttatgagg agatcgccga
    10801 gatcatggag tgcccggtcg gcacggtgcg atcaaggatt ttccgcgccc gcgaggccat
    10861 tgaccagcgc atcgccgcca tggttcagta gccacgaaaa acaagaagat gcagaaaaaa
    10921 accaaccgaa accagcacgc ccgccccgat gctcggcacc gggtgcagcg cgttgacacc
    10981 acaatttctg tcttgccatg gcactttcac cgagttgccg tgtcaaacgg gggtcaatga
    11041 tgcaacttgg ggtcactgag atgaacgaag cgatcgagga gtcgctgtca gccttgctgg
    11101 atggcgaggc cgatgaactg gagaccatgc gggtgctgcg cgcgctcgaa accgatccgg
    11161 aactgcgcga aaagtggcgc cgctaccata tgatggtgac cttgctgcgt ggcgagcaga
    11221 aggtctcgag ccgactcgac atcggccgca aggtgaccct ctcgctggcc ggagagtcgg
    11281 tggcgcaacg caatcgatcc gagcagaaaa cccgcccctg gtcgaagtcg gtcgccattc
    11341 tggcagtggc tgcctcgacc accttcatcg tggtctttgg tgcgcagcag tggggtcgaa
    11401 tcgacaacag ccaaaatccc gcaaccctgg ccgcccagaa cgccacccca accaccagcg
    11461 ccagcacagc cccgactcat ctccagccca ccaccaccga aaccgcactc gccaccgacc
    11521 aggaacgcct cgaacggcac atgtcccgcc atgcccaagg cagactgggc agcgaaccca
    11581 ccctcaccca acccttcgcc cgtccagcca gtctcgatca acagtgattc cctcctctct
    11641 tcgctgggtc ctgctgcgcg gctggccact gctgctgccg ctatgccttg cctcctcagc
    11701 actctgggca gagagcgccg acgaatggct gctgcgcatg ggtcaggcct ttcgggaaca
    11761 gaactacgat ggcaccttca tctacgtgcg gggagatgaa ctgcaaagct tccgcatcat
    11821 ccaccgcgcc gatgccggca tcgagcagga gcggctgatc cacctcgatg gcgccgcgca
    11881 tgaactgctg cgcactggcg aaggtctctt tgccatccag gccgacgctc caatcagcga
    11941 aaccgcagcc aaggcctcct ttgcccaatc ctttgcccgc gagcccggcc aactggccca
    12001 gcactaccgg ctcgagttgc tgggcgatga acgggtcagc aaccggcagt gcagacgcat
    12061 cgccattcgg ccacatgacc cggaccgcta cagccatgag ctgtggatcg accaggcgtc
    12121 cggtctgctg ctgcgctcgc tgctgcgtga taccgatggc cgcacactgg agcgcttcca
    12181 gttcgtcacg ctacgagtgg ccgatgatgt gagcgtgggc gacctgacat cgatgaccca
    12241 tggctccacc accctgcacc gagtacaccc ca
//
LOCUS       DAORCQ010000069        12029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3503_length_12029_cov_5.427927, whole genome shotgun sequence.
ACCESSION   DAORCQ010000069 DAORCQ010000000
VERSION     DAORCQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12029
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3503_length_12029_cov_5.427927"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKI23_08145"
     CDS             complement(<1..528)
                     /locus_tag="PKI23_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNN36948.1"
                     /translation="MAIVGKPLPAWIDPLLWAVVPLPLLWASSRLLLGWGVDPGERLV
                     HFLGLWSLHWLILTLAITPATWLLHWPHLVRLRRTLGLACLIYASLHLLAYGWFLLGW
                     QFATLGDDLLKRPYIVVGFTAWLLLLPLGATSTRAMQRRLGRRWKTLHRLIYLIALLA
                     LLHLGWQVKADWSDFA"
     gene            complement(531..1526)
                     /gene="msrP"
                     /locus_tag="PKI23_08150"
     CDS             complement(531..1526)
                     /gene="msrP"
                     /locus_tag="PKI23_08150"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNN36949.1"
                     /translation="MLIKKRPDLAEHQVTDETLYHARRDFLARCGHLTGLSLFGGLLG
                     CGDEASSATQTPQWLSDQLAQAQPPQPAFTTDETRTPFEDVTRYNNFYEFGSDKGDPA
                     RHSDRFKPHPWSVVVEGACQKPGRYALEDLVKPHRLEERIYRLRCVEAWSMVVPWVGL
                     PLASLLRRFEPDSRARFVRFETLFDPQQMPGQAATFAAIDYPYVEGLRIDEAMHPLAF
                     IAVGLYGKPLPAQNGAPLRLVVPWKYGFKSIKSIVRIEFVETQPLTTWERSAPSEYGF
                     YANVNPAVDHPRWSQKSERRLPGSLFSPRVIPTRPFNGYEEVASLYSGMDLRRLF"
     gene            complement(1563..2414)
                     /gene="pssA"
                     /locus_tag="PKI23_08155"
     CDS             complement(1563..2414)
                     /gene="pssA"
                     /locus_tag="PKI23_08155"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNN36950.1"
                     /translation="MNEEHPTAEERPNPSLPTDGIERLLPVDEHIEEELAASGHRVRR
                     RGIFLLPNLLTTGALFAGFYSIISAINGQFEAAAIAIFFAMVLDGLDGRVARAVHAQS
                     AFGAEYDSLCDMVSFGVAPALLAFTWGVSQLGKVGLAAAFIYSACAALRLARFNTQIA
                     VADKRYFTGLASPAAAALLSAWVWSGYELFPAAAQVPRALAWLTALLTFMTGLAMVLR
                     VRYHSFKQFDLGGRVPFVLLLVVVLAFFVIALYPARMLLLGFALYALSGPLITLWRRR
                     KQQMSDS"
     gene            complement(2567..3580)
                     /gene="ilvC"
                     /locus_tag="PKI23_08160"
     CDS             complement(2567..3580)
                     /gene="ilvC"
                     /locus_tag="PKI23_08160"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNN36951.1"
                     /translation="MKIYYDKDADLALIQGKKVVIIGYGSQGHAHANNLKDSGVKVVV
                     ALRPGSASASKAEKAGLTVQPIEQAVADADLVMLLAPDEHQAALYRQIEPKLRKGAAL
                     AFAHGFNIHYRQIEPRADLDVIMVAPKGPGHLVRSTYTQGGGVPSLIAIHQDATGQAR
                     NLALSYASANGGGRAGIIETSFREETETDLFGEQAVLCGGASALVQAGFETLVEAGYA
                     PEMAYFECLHELKLIVDLMYEGGIANMRYSISNTAEYGDLTRGPRIITDETKREMKKI
                     LTEVQNGEFAREFILENQAGAATLKAKRRLGENHPIEEVGARLRSMMPWIQANRLVNK
                     EHN"
     gene            complement(3672..4163)
                     /gene="ilvN"
                     /locus_tag="PKI23_08165"
     CDS             complement(3672..4163)
                     /gene="ilvN"
                     /locus_tag="PKI23_08165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNN36952.1"
                     /translation="MRRIISILLENEAGALSRVVGLFSQRGYNIETLTVAPTEDPTLS
                     RLTLTTRGDDRVIEQITKQLNKLIEVVKLVDLSEGEHLERDLLLLKVKAVGPQREEIK
                     RCVDIFRGQIIDITSTLYTVQLVGTAQKIDAFIAALGEAQILEVARTGVSGIARGEKI
                     LAL"
     gene            complement(4165..5886)
                     /locus_tag="PKI23_08170"
     CDS             complement(4165..5886)
                     /locus_tag="PKI23_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HNN36953.1"
                     /translation="MEYLSGAEMVIRFLQDEGVEYIYGYPGGAALHIYDALFRQSRVK
                     HVLVRHEQAATHMADGYARATGKPGVVLVTSGPGATNTITGIATAYMDSIPMVVIAGQ
                     VMSHLIGDDAFQETDMMGICRPVVKHSFSVRRPEEIPLVLKKAFHIASTGRPGPVVVD
                     IPKDMTTPSEKFPYVYPQKVKLRSYNPPQRGHAGQIRKAVDLLLGAQRPVIYSGGGVI
                     MGGAAEALTRLAKLLGYPVTNTLMGLGAFPGTDPQFIGMLGMHGSYEANMAMHHCDAL
                     LAIGARFDDRVTNHTGKFCPQAKIAHIDIDPASISKTIPVDVPIVGPVESVLREMITL
                     IEESTQKPDATALKGWWAQINEWRKRHGGRYRTDDTTLIKPQTVIEALYRVTDGDAYI
                     TSDVGQHQMFAAQYYKFDKPHRWINSGGLGTMGFGFPAAMGIKLNFPDQTVACVTGEG
                     SIQMNIQELSTCMQYNIPVKIINLNNGSLGMVRQWQDMNYESRHSHSYMESLPDFIKL
                     AEAYGHVGIRVDRREDLEPALERCFAMKDRLVFMDIYVDPEEHVYPMHVPQGSMRDMW
                     LSKTERT"
     gene            6355..6798
                     /locus_tag="PKI23_08175"
     CDS             6355..6798
                     /locus_tag="PKI23_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36954.1"
                     /translation="MSVLRRTRLCLLLSLLMAPLANGEKQILYRWSEGDQQHFSDHPP
                     PLGTPYEKVVRQTTPAQQKPEAAAEKPGADQGDAAKPPAADASEQKIRQENCNNARSE
                     LRQLENSARVREVDPQGGSRMLSEEEKNARIERARQMIRDHCVAE"
     gene            complement(6829..9396)
                     /gene="clpB"
                     /locus_tag="PKI23_08180"
     CDS             complement(6829..9396)
                     /gene="clpB"
                     /locus_tag="PKI23_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNN36955.1"
                     /translation="MRDRFTSALQQALVEAQSLAVGRDHGYIEPAHLLLALVDQSGGT
                     TRTLLNSAGANLATLRSALERALDQLARIKQPTGDVKVSPDLARLLNLADRLAQQRQD
                     QFISSELIPLAAFEERGAIAKLLQEAGVTKERLTAAIDALRGGEKVTDPEAEERRQAL
                     ERFTVDLTAKAASGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVNGEVPEGLKEKRILALDMGLLIAGAKFRGEFEERLKGVLNEVTRQEGQIILFI
                     DEIHTVVGAGKAEGSMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQK
                     VFVGEPSEEDTIAILRGLKDRYELHHGVDITDSAIIAAARLSQRYITDRQLPDKAIDL
                     IDEAASRIRMEIDSKPEELDRLERRLIQLKIEAEALKKEEDPASRERLGKLREQISSV
                     ERDYADLEEIWKSEKAGLQGAQQIKSELDQARIDFDAARRQGDLTRMSELQYGIIPEL
                     EKRLASADSQQPAETKLVRNRVTDEEIAEVISKWTGIPVAKMLEGEREKLLRMEEALH
                     QRVIGQDEAVVAVSNAVRRSRAGLADPNRPNGSFLFLGPTGVGKTELCKALASFLFDT
                     EEAMVRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRRPYSVLLLDEVEKAH
                     PDVFNILLQVLEDGRLTDGHGRTVDFRNTVVVMTSNLGSDLIQTLAGEAHYNEMRDAV
                     MGVVERHFRPEFINRIDEVVVFHPLGEAQIRAIARIQLAALERRLAERDLSLHVDDAV
                     IDRLVAVGFDPVYGARPLKRAIQRWIENPLAQALLGGRFAPGAQIRAVIEAGEIRFD"
     gene            complement(9485..10228)
                     /gene="pgeF"
                     /locus_tag="PKI23_08185"
     CDS             complement(9485..10228)
                     /gene="pgeF"
                     /locus_tag="PKI23_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNN36956.1"
                     /translation="MQPEPTLLWADWPELPGVQAGVTLRQPGHSAGSYAGCNLADHVG
                     DDPVRVERNRRQLEQRLGLRAPIDYLTQVHGIEVALLPTASPRPVADAAIRRTPGVAA
                     VLTADCLPILLAAETGDEVAALHAGWRGLAAGVIEATFEKLRAPPRSLQVWLGPAIGP
                     CHFEVGSEVRAAFVARQPADAEAFTLHGDRWRADLYLLARRRLQRLGVTRIFGGGLCT
                     HCDGARFYSYRRDGTTGRMASLIQLHSPA"
     gene            complement(10218..11174)
                     /gene="rluD"
                     /locus_tag="PKI23_08190"
     CDS             complement(10218..11174)
                     /gene="rluD"
                     /locus_tag="PKI23_08190"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNN36957.1"
                     /translation="MSETCIRQQLRIPIELAGLRLDQAAAQLFPDHSRARLQGWISDG
                     QLTVNQRRMRPRDRVVGGEELSLDAVLEAQGDWQAQPIEFRVLFEDDDLLVIDKPAGL
                     VVHPAAGNWEGTLLNGLLHRIPQLIQLPRAGIVHRLDKETSGLMVVAKSLRAHTSLVG
                     QLQNRSMGRDYEAVAEGAMIAGGTVDRPIGRHPRQRTRMAVVATGKPAVTHYRLLHRF
                     RHYSHLRLSLETGRTHQIRVHMAELHHPLVGDRDYGGRLRLPAGLAPALIEVIRAFPR
                     QALHAKALRLTHPHSGETLQWEVPLPADMQQLLEQLAQHDAA"
     gene            11325..>12029
                     /locus_tag="PKI23_08195"
     CDS             11325..>12029
                     /locus_tag="PKI23_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNN36958.1"
                     /translation="MLLLLLALTTLLGGCSLFGEKKEKPELSEQEYYNQARKALDVNN
                     IQEAIELLQALESRYPFGAYGQQGQLELIYAQYRSFDYDSAAIAADRFIRLHPTHANV
                     DYAYYMKGLANYDRDDMGFTRYLSQNPAERDLTNARQAYLDFSQLVNRFPTSPYAADA
                     RQRMIFLRNQLAEREIVIARYYFKRKAYLAAANRGRYLVENYPDSPLVGDGLAVMAEG
                     YRLMELPALQADAVEVL"
BASE COUNT         2009 a         3798 c         4041 g         2181 t
ORIGIN      
        1 cgcgaagtcg ctccagtccg ccttgacctg ccagccgagg tgcagcagcg ccagcagggc
       61 gatcaggtag atcagccgat ggagcgtttt ccagcggcgt cccagccgtc gctgcatcgc
      121 gcgggtcgag gtggcgccca gcggcaacag cagcagccag gcggtgaagc cgacgacgat
      181 gtagggccgc ttgagcaggt catcacccag ggtcgcaaac tgccagccga gcagaaacca
      241 gccataggcc agcagatgca gcgaggcgta gatcaggcag gccaggccca gcgtccggcg
      301 caggcgaacc aggtgtggcc agtgcagcag ccaggtggcc ggggtgatcg ccagtgtcag
      361 gatcagccag tgcaggctcc acagaccgag aaaatggacc agccgttcac ccggatcgac
      421 gccccagccg agcagcagcc ggctgctggc ccacagcagc ggcagcggca ccacggccca
      481 cagcagcgga tcgatccagg ctggcagcgg cttgccgacg atggccatgc tcagaagagc
      541 cggcgcaggt ccatgccgct gtagagcgag gcgacctctt catagccgtt gaacgggcgg
      601 gtcggaatga cgcggggtga gaagagcgat ccgggcagac ggcgctcgct cttctggctc
      661 cagcgcggat ggtccaccgc cggattgacg ttggcataga agccatattc gctgggtgcc
      721 gacctctccc aggtggtcag tggttgggtc tcgacgaatt cgatgcgcac gatcgacttg
      781 atgctcttga agccatactt ccacggcacc accagccgca gtggcgcgcc gttctgtgcc
      841 ggcagcggct tgccatagag gccgacggcg atgaaggcga gcggatgcat cgcctcgtcg
      901 atgcgcagcc cttcgacgta gggatagtcg atggcggcga aggtggcggc ctgtcccggc
      961 atctgctgcg ggtcgaacag cgtctcgaag cggacgaagc gggcgcggga gtcgggttcg
     1021 aagcggcgca gcagggacgc cagcggcagt ccgacccagg gcaccaccat cgaccaggcc
     1081 tcgacgcagc gcagccggta gatgcgctct tcgagccgat gcggcttcac cagatcttcg
     1141 agtgcatagc ggccgggttt ctggcaggcc ccctcgacga ccactgacca ggggtgcggt
     1201 ttgaagcggt cgctgtggcg ggccggatcg cccttgtcgc tgccgaattc gtagaagttg
     1261 ttgtagcggg tcacatcctc gaagggcgtg cgcgtctcat cggtggtgaa ggcgggctgt
     1321 ggcggctgcg cctgagcgag ctgatcactc agccactgcg gcgtctgcgt cgcggagctg
     1381 gcctcgtcgc cgcagccgag cagaccgccg aacaggctca ggccggtcag gtggccgcag
     1441 cgggcgagaa agtcacgccg ggcgtggtag agggtttcgt cggtgacctg atgttccgca
     1501 aggtcggggc gcttcttgat cagcatgctc gaactcctgc acagggccgg ggcgggtttc
     1561 gtctagctgt cggacatctg ctgcttgcgg cggcgccaca gggtgatcaa cgggccggag
     1621 agggcataga gtgcaaagcc gagcagcagc atgcgcgccg ggtagagcgc gatcacgaag
     1681 aacgcgagca ccaccaccag cagcagcacg aaggggacgc gtccgccaag gtcgaactgt
     1741 ttgaacgagt gatagcgcac gcgcagcacc atcgccagac cggtcatgaa ggtgagcagg
     1801 gcagtcagcc aggcgagcgc gcggggcacc tgcgccgcag cgggaaagag ctcgtacccg
     1861 ctccagaccc aggcgctgag cagggccgcc gccgccggac tggccagacc agtgaagtag
     1921 cgcttgtcgg ccaccgcgat ctgggtgttg aagcgggcca gtcgcagtgc cgcacaggcg
     1981 ctgtagatga aggcggcggc cagcccgacc ttgcccagtt gcgacacgcc ccaggtgaag
     2041 gccagcagtg ccggtgcaac cccgaacgag accatgtcgc acaggctgtc atattcggca
     2101 ccgaaggcac tctgggcatg gacggcgcgg gccacccggc catcgagccc atcgagcacc
     2161 atcgcaaaga agatggcgat ggcagccgct tcgaactggc cattgatggc gctgatgatc
     2221 gagtagaagc cggcgaacag cgcaccggtg gtgagcaggt tgggcagcag gaagatgccg
     2281 cgccgccgca cccgatggcc gctggcggcc agctcctctt cgatgtgttc atcgaccggc
     2341 agcagccgtt cgatgccatc ggtggggagg gatgggttcg gacgttcttc agcggtgggg
     2401 tgctcttcat tcatgtatcg ggcctgtcgc tgcggcatcc atcgcactgc ctgttgccgg
     2461 tcggggccat gctacaccag ccgggggctg gttttgcagc gcccggggtt gcgggcggaa
     2521 acaatgaagg cagaggcatt gcggcccctg ccttcatggt cggcactcag ttgtgctctt
     2581 tgttgaccag gcggttggcc tgaatccacg gcatcatcga gcgcagccgg gcacccacct
     2641 cttcgatcgg gtggttctcg cccaggcgac gcttggcctt cagggtggcg gcgccggcct
     2701 gattttccag gatgaactcg cgggcgaact cgccattctg cacctcggtg agaatctttt
     2761 tcatctcccg cttggtctcg tcggtgatga tgcgtggccc gcgggtcagg tcaccatact
     2821 cggcggtgtt ggagatcgag tagcgcatgt tggcgatgcc gccctcatac atcaggtcga
     2881 cgatcagctt gagctcgtgc aggcattcga aataggccat ctcgggggca tagccggcct
     2941 cgaccagcgt ctcgaagccc gcctgcacca gtgcgctggc gccaccacag agcacggcct
     3001 gctcgccgaa caggtcggtc tcggtctctt cgcggaagct ggtctcgatg atgccggccc
     3061 gtccgccgcc attggccgag gcgtaggaga gcgccagatt gcgtgcctga ccggtggcat
     3121 cctgatggat cgcgatcagg ctgggcacgc cgccgccctg ggtgtaggtg gagcggacca
     3181 gatggccggg ccccttgggg gcgaccatga tcacgtcgag gtcggcgcgc ggctcgatct
     3241 gccggtagtg gatgttgaag ccgtgggcga aggccagtgc cgcgcccttg cgcagcttgg
     3301 gctcgatctg tcggtagagc gcggcctgat gctcatcggg tgccagcagc atcaccagat
     3361 cggcgtcggc gaccgcctgc tcgatcggct gcacggtcag gccggccttt tcggccttgc
     3421 tggcggaggc cgagccgggg cgcagtgcca ccaccacctt cacgccggaa tccttcaggt
     3481 tgttggcatg ggcgtggccc tgcgagccgt agccgatgat caccaccttt ttgccttgaa
     3541 tcagcgcaag gtcggcatct ttgtcgtagt agatcttcat caggtcatcc tcggtcaatg
     3601 ctcaggggca gggtgcatcg ctgtgccctg cggggtcaac ggaaaattgc agcagcggct
     3661 catgccgccg gctacagggc cagaatcttc tcgccacggg cgatgcccga gacgccggtg
     3721 cgtgccactt cgagaatctg cgcttcgccg agtgcggcga tgaaggcgtc gatcttctgt
     3781 gcggtgccga ccagttgcac ggtatagagc gtgctggtga tgtcgatgat ctggccgcga
     3841 aagatgtcga cgcagcgctt gatctcctcg cgctgcggac cgaccgcctt caccttcagc
     3901 agcagcaggt cgcgttcgag gtgctcaccc tcgctcaggt cgaccagctt gaccacctcg
     3961 atcagcttgt tgagctgctt ggtgatctgc tcgatcaccc ggtcatcgcc gcgggtggtc
     4021 agcgtcagcc gcgacagtgt cggatcttcg gtcggagcca ccgtcagggt ttcgatgttg
     4081 tagccgcgtt gcgaaaagag cccgacgacc cgtgacaggg cgccggcttc gttttccagc
     4141 agaatggata tgattcttcg catctcaggt ccgctccgtc ttgctcagcc acatgtcgcg
     4201 catcgagccc tgcggcacat gcatcgggta gacatgctcc tcggggtcga catagatgtc
     4261 catgaagacc agccggtctt tcatggcgaa gcagcgttcg agcgccggtt ccagatcttc
     4321 ccgccggtcg acgcgaatgc cgacatggcc gtaggcctcc gccagtttga tgaagtcggg
     4381 cagcgactcc atgtaggagt gggagtggcg gctctcgtag ttcatgtcct gccactgccg
     4441 caccatgccg agcgagccgt tgttgaggtt gatgatcttg accggaatgt tgtactgcat
     4501 gcaggtcgag agctcctgga tgttcatctg gatgctcccc tcgccggtca cgcaggcgac
     4561 ggtctggtcc ggaaagttga gcttgatgcc catcgccgcc ggaaagccga agcccatcgt
     4621 ccccagcccg ccggagttga tccagcggtg gggtttgtcg aacttgtagt actgggccgc
     4681 gaacatctga tgctggccga catccgaggt gatgtaggcg tcgccgtcgg tgacgcggta
     4741 gagcgcctcg atcaccgtct gcggcttgat cagggtggtg tcgtcggtgc ggtagcggcc
     4801 gccatgccgt ttgcgccact cgttgatctg tgcccaccag cccttcagcg cggtggcatc
     4861 gggcttctgc gtgctctcct cgatgagtgt gatcatctcc cgcagcaccg actcgaccgg
     4921 cccgacgatc ggaacgtcga ccggtatcgt cttggagatc gaggcaggat cgatgtcgat
     4981 gtgggcgatt tttgcctgcg ggcagaattt gccggtgtga ttggtgaccc ggtcgtcgaa
     5041 gcgggcgcca atcgccagca gcgcgtcgca gtggtgcatc gccatgttgg cctcatagct
     5101 gccatgcatg cccaacatgc cgatgaactg cggatcggtg ccaggaaagg cgcccagccc
     5161 catcagggtg ttggtgaccg ggtagcccag cagctttgcc agccgggtga gggcttcggc
     5221 cgcgccgccc atgatcacgc cgccaccgct gtagatcacc ggccgctggg cgccgagcag
     5281 caggtcgacc gccttgcgga tctgcccggc atggccgcgc tggggcgggt tgtaggagcg
     5341 cagtttcacc ttctgcggat agacataggg gaacttctcg ctcggcgtgg tcatgtcctt
     5401 cggaatgtcg accaccaccg gaccgggccg gccggtgctg gcgatgtgga acgccttttt
     5461 cagcaccagc ggaatctcct ccggccggcg caccgagaag ctgtgcttca ccaccggtcg
     5521 gcagatgccc atcatgtcgg tctcctgaaa ggcgtcatcg ccgatcaggt ggctcatcac
     5581 ctgaccggcg atgaccacca tcgggatcga atccatgtag gcggtggcga tgccggtgat
     5641 ggtgttggtg gcgccagggc cggaggtgac cagcaccacg ccgggcttgc cggtggcacg
     5701 ggcatagcca tcggccatgt gggtggcggc ctgttcgtgc ctgaccagca catgcttgac
     5761 ccggctctgc cggaacagcg catcataaat atgcagggcg gctccgcccg gatagccgta
     5821 aatgtattcg accccctcat cctgaagaaa gcggatgacc atttcagcgc ctgacaagta
     5881 ctccatctgt gctacctcac aagtacctta gggtatgtcg atgatcgatt gctgttggat
     5941 atggccgaca ccttcgtcgc ggtggatcaa aggtgttggc gcgtgccggt gccgggtaga
     6001 tggccagaag gggcaccgat cgcgggggtg ggcggtcggt tgccgggaag gctcagggtg
     6061 aaatcagtgc cgacggggtt cagcccggtt cggcagcgac gaatcatcag gtgcgggggt
     6121 aaccacaccg ccctgcaccc tgtacagagc gggtctggca ttctctcttg ccggtgctct
     6181 ttgtcaaggg gcagtgtcga ctgctggcct gtttcggccg aaaagcgcaa gccagggtct
     6241 cgtttcgggg cggttggcgg gcaaatggaa ggtggagctg gcggttttgc cctattatct
     6301 gataattgaa agattctgtt gcgcatgaat ctcgattcca aaggatgccg ctgcatgtcc
     6361 gttttgcgaa gaacgcgcct ctgtctgctg ctgtcgctgc tgatggcgcc gttggccaac
     6421 ggcgagaaac agattctcta ccgctggagc gagggcgatc agcagcactt cagcgaccat
     6481 ccgccgccgt tgggcacccc gtacgagaag gtcgtgcggc agacgacacc tgctcagcaa
     6541 aagcccgaag ccgccgccga gaaacccggt gcagatcagg gtgatgctgc caagccgccg
     6601 gccgccgatg ccagtgagca gaagatccgt caggagaact gcaacaacgc ccgcagcgag
     6661 ctgcgccagc tcgaaaacag cgcccgggtg cgcgaggtcg atccacaggg tggctcgcgg
     6721 atgctgagcg aagaggagaa gaatgcccgc atcgagcggg cacggcagat gatccgcgac
     6781 cattgcgtcg ccgagtgatc gacgacgcaa tggcgggttg ggcgggcgtc agtcgaagcg
     6841 gatctctccg gcctcgatca ccgccctgat ctgcgcgccc ggcgcgaaac ggccacccag
     6901 cagtgcctgc gccagcgggt tctcgatcca gcgctgaatc gcccgcttca gcggccgcgc
     6961 cccatagacc gggtcgaaac cgaccgccac cagtcgatcg atgacggcat catcgacatg
     7021 cagtgacaga tcgcgctcgg cgagccgccg ctccagtgct gcgagctgga tgcgggcgat
     7081 ggcgcgaatc tgcgcctcac ccaatggatg gaacaccacc acctcatcga tgcggttgat
     7141 gaattcgggg cggaaatggc gctcgaccac gcccatcacc gcgtcgcgca tctcgttgta
     7201 gtgggcttcg ccggccaatg tctggatcag gtctgagccg aggttcgagg tcatcaccac
     7261 cacggtgttg cgaaaatcga cggtgcggcc atggccgtcg gtcaggcgtc catcctccag
     7321 cacctgcagc aggatgttga agacatcggg gtgggccttc tcgacctcat cgagcagcag
     7381 caccgagtag gggcggcggc gcaccgcctc ggtcagatag cccccctcct catagccgac
     7441 atagcccggc ggtgcgccga tcagccgcga caccgagtgc ttctccatga actcggacat
     7501 gtcgatccgc accatcgcct cttcggtgtc gaacaggaag ctggcgagtg ccttgcacag
     7561 ctcggtcttg ccgacaccgg tcgggccaag gaacaggaag gagccattcg ggcggttcgg
     7621 gtcggccagt ccggcgcgcg aacggcgtac ggcgttggag acggcgacca ccgcctcgtc
     7681 ctgaccgatc acccgctggt gcagcgcctc ttccatccgc aaaagcttct cgcgctcgcc
     7741 ctccagcatc ttggcgaccg gaatgccggt ccatttggag atcacctcgg cgatctcctc
     7801 gtcggtgacc cggttgcgca ccagtttggt ctcggccggc tgctgactgt cggccgaggc
     7861 cagccgcttc tccagttcgg gaatgatgcc gtattgcagc tccgacatgc gtgtcaggtc
     7921 accctgacgg cgcgcggcat cgaagtcgat gcgggcctgg tccagttccg acttgatctg
     7981 ctgtgccccc tgcaggccgg ccttctcgct cttccagatc tcctcgaggt cggcatagtc
     8041 gcgctcgacg ctgctgatct gctcgcgcag cttgccgagc cgctcgcgtg aggcggggtc
     8101 ctcttctttc ttcagcgcct cggcctcgat cttgagctgg atcagccggc gctccagccg
     8161 atcgagctcc tcgggcttgg agtcgatctc catgcggatg cggctggcgg cctcgtcgat
     8221 caggtcgatc gccttgtcgg gcagttgccg gtcggtgatg tagcgctgcg acaggcgggc
     8281 ggcggcgatg atggccgagt cggtgatgtc gacgccatgg tgcagttcat aacggtcttt
     8341 cagtccgcgc aggatggcga tggtgtcctc ctcgctcggc tcgccgacga agactttctg
     8401 gaagcggcgt tcgagtgcgg catccttctc gatgtactgg cggtactcat cgagcgtggt
     8461 ggcgccgatg cagtgcagtt caccccgcgc cagcgccggt ttgagcatgt tgccggcatc
     8521 catcgaaccc tctgccttgc cggcgccgac cacggtatga atctcgtcga tgaagaggat
     8581 gatctgcccc tcctgccggg tcacttcatt gagcaccccc ttgagccgct cctcgaactc
     8641 gccacggaac ttggcaccgg cgatcagcag tcccatgtcg agcgccagaa tccgcttctc
     8701 cttcagcccc tcgggcacct cgccattgac gatgcgctgt gccagccctt cgacgatcgc
     8761 cgtcttgccg acgccgggtt cgccgatcag caccgggttg ttcttggtgc ggcgctgcaa
     8821 cacctggatg gtgcggcgga tttcgtcgtc ccggccgatc accgggtcga gcttgccgct
     8881 ggccgccttg gcggtgaggt cgacggtgaa gcgctccagt gcctggcgcc gctcctcggc
     8941 ctcggggtcg gtcaccttct cgccgccacg cagcgcatcg atggctgcgg tcagccgctc
     9001 cttggtgacg ccagcctcct gcaacagctt ggcgattgcc ccgcgctctt cgaaggcggc
     9061 gagcggaatc agctcgctgg agatgaactg gtcctgacgc tgctgggcca gcctgtcggc
     9121 caggttcagc aggcgggcca gatcgggaga gaccttcaca tcgccggtcg gctgcttgat
     9181 tctggccaat tgatcgagtg cccgctccag cgcactgcgc agtgtggcga ggttggcgcc
     9241 ggcgctgttc agcagcgtgc gggtggtgcc accgctctga tcgaccagcg cgagcagcag
     9301 gtgggcgggt tcgatgtagc catggtcacg gcccaccgcc agggactggg cttcgacgag
     9361 ggcctgttgc agggcgctgg tgaatcggtc gcgcatggag cttctccttc aaagggcttg
     9421 agtccggtgc accaaccgct ggtgcacggc attgtccctg tagatggggt gggaacgcgg
     9481 cgattcaagc cggggagtgc agctggatca ggctggccat gcggccggtg gtgccatcgc
     9541 ggcggtagga gtagaagcgc gcaccatcgc agtgggtgca gagaccgcca ccgaagatgc
     9601 gggtcacgcc caaccgctgc agccgccggc gcgccagcag gtagagatcg gcccgccagc
     9661 ggtcgccgtg gagggtgaag gcctcggcat cggctggctg ccgggccacg aaggcggcgc
     9721 gcacctcgct gccgacctcg aagtgacagg ggccgatggc cggtcccagc cagacttgca
     9781 ggctccgggg cggcgcgcgc agcttttcga acgtggcttc gatgacgccg gcggccaggc
     9841 cgcgccagcc ggcatgcagt gcggccacct catcgccggt ttcggcagcg agcagaatcg
     9901 gcagacagtc ggcggtcagc accgcggcaa cgccgggcgt gcggcggatg gcagcatcgg
     9961 caaccgggcg tggtgacgcg gtcggcagca gcgcgacctc gatgccatgc acctgggtca
    10021 gatagtcgat cggcgcgcgg agcccaagcc gctgctccag ttgccggcgg ttgcgctcga
    10081 cccgaaccgg atcgtcgccg acatgatccg ccaggttgca gccggcatag gagccggcac
    10141 tgtggcccgg ttggcgcagc gtgacgccgg cctgcacgcc cggcagttcc ggccagtcgg
    10201 cccacagcag ggtcggttca ggctgcatcg tgctgtgcca actgttccag cagttgctgc
    10261 atgtctgccg gcagcggcac ctcccactgc agggtttcac cgctgtgggg gtgggtcagc
    10321 cgcagcgcct tggcatgcag tgcctgccgt ggaaaggcgc ggatcacctc aatcagtgcg
    10381 ggcgccaggc ccgccggcag ccgcaaacga ccgccgtagt cgcggtcacc gaccagcgga
    10441 tggtgcagtt cggccatgtg cacccggatc tggtgggtgc ggccggtctc cagcgacagt
    10501 cgcaggtggc tgtagtggcg aaagcggtgc aggaggcggt agtgggtcac ggccggcttg
    10561 ccggtcgcga ccaccgccat cctggtgcgc tgtcgcggat ggcggccgat cggtcgatcg
    10621 accgtgccgc ctgcgatcat cgcgccttcg gccaccgcct cgtagtcgcg gcccatgctg
    10681 cggttctgga gctgcccgac cagcgaggtg tgcgcgcgca ggctcttggc caccaccatc
    10741 aatccggagg tctccttgtc gaggcgatgc acgatgccgg cgcggggcaa ctggatcagt
    10801 tgcggaatgc ggtgcagcag accattgagc agggtgccct cccagttgcc ggcggcgggg
    10861 tggaccacca gtccggccgg cttgtcgatc accagcaggt cgtcatcctc gaacagcacc
    10921 ctgaattcga tcggttgagc ctgccaatcg ccctgcgctt cgagcaccgc gtcgaggctg
    10981 agctcctcgc cgccgacgac acggtcgcgc ggtcgcatcc gccgctgatt gacggtcagt
    11041 tggccgtcac tgatccagcc ttgcagccgt gcgcgcgagt gatcgggaaa gagttgcgcc
    11101 gcggcctgat cgagacgcag cccggccagt tcgatcggaa tgcgcagttg ctgcctgatg
    11161 caggtttcac tcatggcgaa ggggtgtcgg ggccgacggc gattggttca gccggggcgc
    11221 tatggttaaa tggcggcttg cctgtttcat ctccttctcg atttctcacc atcgtgattt
    11281 caaaccgcac tttcgatcat cgcattgacc cccttcccgc tgtcgtgctc ctgctgctgt
    11341 tggcgctgac cacgctgctg ggtggctgct ccctgtttgg cgagaagaag gagaagcccg
    11401 aactgagcga gcaggagtac tataaccagg cgcgcaaggc gctcgatgtg aacaatatac
    11461 aggaagccat cgagctgctg caggcgctcg agtcgcgcta tccgttcggc gcctacggcc
    11521 agcaggggca actggagctg atctatgccc agtaccgcag cttcgactat gacagcgcgg
    11581 cgattgccgc cgaccgtttc atccggctgc acccgaccca cgccaatgtc gactacgcct
    11641 actacatgaa ggggctggcc aactacgacc gcgacgacat gggctttacc cgctatctgt
    11701 cgcagaatcc ggcagaacgc gatctgacca acgcccgcca ggcctacctc gatttttcgc
    11761 agctggtcaa ccgctttccg accagtccct atgccgccga cgcccggcag cggatgatct
    11821 ttctgcgcaa ccagctggcc gagcgggaga tcgtcatcgc ccgctactat ttcaagcgca
    11881 aggcctatct ggccgccgcc aaccggggcc gttacctggt cgagaactat cctgactcgc
    11941 cactggtcgg tgacgggctg gcggtgatgg ccgagggcta ccggctgatg gagctgccgg
    12001 cactgcaggc cgacgccgtc gaggtgctg
//
LOCUS       DAORCQ010000070        12004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3510_length_12004_cov_5.272073, whole genome shotgun sequence.
ACCESSION   DAORCQ010000070 DAORCQ010000000
VERSION     DAORCQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3510_length_12004_cov_5.272073"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKI23_08200"
     CDS             <1..263
                     /locus_tag="PKI23_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNN36959.1"
                     /translation="EIEVESLAQLQEAIDAGADIVMLDNFNLEQMHQAVQLTAGRLKL
                     EASGGMTAEQLRAVAATGVDYISLGTLTKDIKAIDLSMRFTL"
     gene            335..865
                     /gene="ppa"
                     /locus_tag="PKI23_08205"
     CDS             335..865
                     /gene="ppa"
                     /locus_tag="PKI23_08205"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNN36960.1"
                     /translation="MSLNTLCAGRDVPNEVHVIIEIPAHSGPVKYEVDKDSSALFVDR
                     FLSAPMFYPCNYGYIPQTLCGDGDPLDVLVIAPHPLISGSVIRCRPIGMMKMVDEAGE
                     DTKLIAVPIHKLTPLYDHVEEIDHVSPQLMAQIEHFFTHYKDLETGKWVRIDGWGNSA
                     EARAEILQSIENYKTA"
     gene            complement(956..1720)
                     /locus_tag="PKI23_08210"
     CDS             complement(956..1720)
                     /locus_tag="PKI23_08210"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNN36961.1"
                     /translation="MNRVAGKVALISGGASGLGKAQALLLAQQGAKVVVTDLNEGDGQ
                     AVADEIVAAGGEAQFLRQDVRNESEWEAVMSATLGRFGRLDVVVNNAGIGVGGNAEDA
                     TLEQWRHLMSINLDAVFLGTKHAIRAMKGRGGSIINISSIEGLVGDPNLAAYNASKGG
                     VRLLTKSAALHCAKAGYGIRVNSVHPGYILTPMVERAMQGEHAELVRQHLVSLHPIGR
                     LGAPLDIAWGVLYLASDESSFMTGSELVIDGGYTAQ"
     gene            1848..2528
                     /locus_tag="PKI23_08215"
     CDS             1848..2528
                     /locus_tag="PKI23_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNN36962.1"
                     /translation="MSGTIIEFSTAAGGNCQGYLSIPAAGKGPAVIVIQEWWGLVDHI
                     KQVADRFAETGFVALAPDLYHGVQARQPDEAGRLMMALNIDGAASDLSGAADFLLEHP
                     AVTSRKVGTVGFCLGGQLSLMAACVDTRIGACVDYYGVHPQIQPDFSKLASPVLGFFG
                     GLDGFVTPDAVSALEQQIRQAGKSVEFHVYPDADHAFFNDSRPEVYHRAHAEESWKRM
                     LAFFHANL"
     gene            complement(2531..3418)
                     /locus_tag="PKI23_08220"
     CDS             complement(2531..3418)
                     /locus_tag="PKI23_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNN36963.1"
                     /translation="MADSPAVRGAAVSDLDRLGFTLFLALAIHGVLLLGISFKANLKP
                     PPESQPTIEVTLATQASARPPPKADFIAPVHQQGSGEAEQAQRLTSPKPATTQSPRQQ
                     LSQSEQAPARPQPRPRSVPPAVSRASQTAKPVPQRAEPTPEVAAPAQDSTLLERSIEI
                     ASLEAELDRQQQVKARRPRVRTISAASTQSSVDAYYLEAWRQKIERVGNLNYPQQARD
                     QQIYGSLRMMVRLLPDGSVDRIQIIESSGHPLLDEAAVRIVRLAEPFAPFPPELSRET
                     DVLQIIRTWRFRNSYSSAP"
     gene            complement(3430..4386)
                     /gene="gshB"
                     /locus_tag="PKI23_08225"
     CDS             complement(3430..4386)
                     /gene="gshB"
                     /locus_tag="PKI23_08225"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNN36964.1"
                     /translation="MPKPLEIGVVMDPIEAVSYKKDSTLAMLWAAQERGWRLHYMEQK
                     DLCLTEGRAMASSRPLEVFRDPQRWFSRGEPQQGPLSRFDAILMRKDPPFDTEYIYTT
                     YLLEQAEREGVLVVNRPQSLRDCNEKMFAATFPELGPPTLVSRQAGQIRDFHRHHGDI
                     IVKPLEGMGGASVFRLTPEDPNVSVILETLTHHGQRYIMAQRYLPEIRNGDKRILLID
                     GEPVPYALARIPAPGESRGNLAAGGTGEGRPLTSRDRWICAQLGPALRERGLIFVGID
                     VIGDCLTEINVTSPTCIRELQNQFSIDIAGQLMDAIARRLGR"
     gene            4632..5021
                     /gene="pilG"
                     /locus_tag="PKI23_08230"
     CDS             4632..5021
                     /gene="pilG"
                     /locus_tag="PKI23_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HNN36965.1"
                     /translation="MDESNFSGLKVMVIDDSKTIRRTAESLLAKVGCEVITATDGFEA
                     LAKIADGRPDIIFVDVMMPRLDGYQTCALIKNNREFRATPVIMLSSKDGLFDKAKGRI
                     VGSDEYLTKPFSKEELMGAIEQHVVRH"
     gene            5033..5446
                     /locus_tag="PKI23_08235"
     CDS             5033..5446
                     /locus_tag="PKI23_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN36966.1"
                     /translation="MGALAYRPSGNDQSAIAARVLIVDDSPTETLAISTMLRRHGYQV
                     LAATSGEEGIETARREQPDLVLMDVVMPGISGFQATRQLTREQATGHIPVVIISTKDQ
                     PTDRVWGLRQGAREYLCKPVLEQRLLQTIEQILTH"
     gene            5464..6012
                     /locus_tag="PKI23_08240"
     CDS             5464..6012
                     /locus_tag="PKI23_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNN36967.1"
                     /translation="MHPPADPADLLLDIERRCQRHAAALPQPTRPERTIDTGIGFLFA
                     GLEMVAAMSEISEILAVPRLSKVPGVKPWVCGIANLRGRLLPIYDIDLLCGHERDRTV
                     QQREQRVLVTECSGQYSGFMVSRIFGMKPMDPDRFESNTPAAPSVLSSCIEGFYRNGE
                     QTWLRLSLHRLAAAPALQQLAR"
     gene            6237..7355
                     /locus_tag="PKI23_08245"
                     /pseudo
     CDS             6237..7355
                     /locus_tag="PKI23_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            7369..>12004
                     /locus_tag="PKI23_08250"
     CDS             7369..>12004
                     /locus_tag="PKI23_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNN36968.1"
                     /translation="MNIASSQPGVTSSLMAARWLLPEIQVELHAIQVLLDQAATDSQS
                     ESEVADRLRQLGNLLTVAELTLPATLCHELRQALQTTRFSLPARELALEILLRLPDLI
                     GELARDEPDALALTWILRAELQRLLGRPLPEPLSRSIGPPAAVALSAEQLAHYPAERF
                     LPLAAKLHATYRDGLLQYRQTNMAAALQGMQRVVGKLLEIAPQSPSSPLLQALLRILA
                     AMEQARLGPRAALLDLLSEFEPWLARLVTTGAALLNQPPTLLAALQPYAQLAGPMDGH
                     DDTTSPLLPISGTGAAREVLLNELAIEIDHLKTRLEENLDLPALHQECERLRSTIRLL
                     ALPDLTRAMQRVIELLSATPERLDRLQLAEAVLELEAAVMNPDSQVAATTQRNSGLRQ
                     AQQQFMQQCAQELEELRQRLDDHLTLPDDNPLPPQVEQGLQQLQGGFNLMEWPELATA
                     IGVARTQLLAPQRPAPADLALIVDLLIGTEFYLQQWLAGERDAPSANLINLCNRLIRA
                     PQEESQATPAPAPAEDASDSGFDAEIAAVFVEEAQEVLAALDEQWPAFAAGAQGALIE
                     VRRGFHTLKGSGRMVAADTVAALAWAVEQRLNQLIDLGHGLSGELIELIDQTRNLLPE
                     LLDCFANGQAAAPGWQQLLQRIEASDNAEETKPEPQARPESATATATAAEESLDAVFS
                     REAALHLQRIEQILAHPDSPQGDWRLLERTLHTLKGSANAAGRRPLAQLAGAMLDAVQ
                     RHLQEGEAAPTLACRAGAELLPMLVLHAGDDATLPPQFHAAVELLQQPLCEPEALSTR
                     WLDAADTLVAALEQLGSAPQQAQARQQIEQAISAALGEQLPAAIRNWIDHLHLWMAQH
                     FHADRIEARARLALCRQALIESIEAEVLNQPASDWSELWQPLPEPQRPPSTPERAFAT
                     PQPEEKSAAAAPMSAATREADEELLAIFMGEAEELLEEIQAIQRHWPDRPAATSEQVA
                     ALARALHTLKGGARLVGQRMLADICHEFETRLDSLRVAEVGPDELSGLVRQLQAVISA
                     EQSTDVDIDSPSTRLETPEVALSVLPPETSTVQATGAEPAEITDPIRSPARGETLRLS
                     ASLLSHLLDLTNESTLLQERLERQSREFGQAVDEMELTIDRLRDQVRRLDIEAESELR
                     LRHRQGSSERQDFDPLELDRYSQLQQLSRGLVESASDIKDLRDAMAGRLQQMRGQLLQ
                     QSHLHTELREGLARAQMVPFSRLVPRLGKMVRQLATELGKDVGLRVINPEVEIDRTVL
                     ERLITPLEHMLRNALDHGIESPAQRLALGKSARASITLQLQPQGSNLLLSVSDDGAGI
                     NVEQVLHKARSRGLIDPQRQLSPNEALQLIFLPGFSTASVVTQISGRGVGMDVVRNEV
                     QALGGTIDASSRPGEGTCFELRLPYTLSANRALMVRVGGELYALPLETIEGVVRVSPY
                     ELEHHYQQPDSRYAYAGRDYRLRHLGTLLFGWNPPTLDGVTRPLPVILLRSQDDAVAL
                     QVDALEGSSEIVAKALAPQFNQVPGIAGASLQGDEGAVIILDLPALLRAKP"
BASE COUNT         2262 a         3955 c         3701 g         2086 t
ORIGIN      
        1 tggagatcga ggtggagagt ctcgcgcagt tgcaggaggc gatcgatgcg ggcgccgaca
       61 tcgtcatgct cgacaacttc aacctcgaac agatgcacca ggccgtgcaa ctcactgccg
      121 ggcgcctcaa gctcgaagcc tccggtggca tgacggccga gcagttgcgt gcggttgccg
      181 cgaccggcgt cgactacatt tcgctgggga cgctgaccaa ggacatcaag gccatcgacc
      241 tctccatgcg gttcaccctc tagaaggttg tcgctatact gcgccgctga cttggccagt
      301 tcactctttt tgccgcacaa caggaccgtt atccatgagt ctcaacaccc tctgcgccgg
      361 gcgcgacgtg cccaatgaag tccacgtcat catcgagatt cccgcccaca gcggaccggt
      421 caaatatgag gtggacaaag actcctcggc actctttgtc gaccgcttcc tctcggcccc
      481 gatgttctac ccctgcaact atggctacat cccgcagacc ctctgcggcg acggcgatcc
      541 gctcgatgtg ctggtgatcg ctccccaccc gctgatcagc ggctcggtga ttcgctgccg
      601 tccgatcggc atgatgaaga tggtcgacga agcgggagaa gacaccaaac tgatcgcagt
      661 gccgattcac aaactgaccc cgctctatga ccatgtcgag gagatcgatc acgtcagccc
      721 gcaactgatg gcgcagatcg agcatttctt cacccactac aaggatctcg aaaccggcaa
      781 gtgggtacgc atcgatggct ggggcaacag cgccgaagcc cgtgccgaga ttctccagtc
      841 gatcgaaaac tacaaaaccg cctgatcacg cttcgaaaaa agccagcgct gccctcatgg
      901 cggcgctggc tttttcattg gccacccggc atggagcgag cctgactgcc tgctgctact
      961 gcgcggtata gccgccatcg atcaccagtt cactgccggt catgaagctc gactcatccg
     1021 aggcgaggta gagcacaccc caggcgatgt cgagcggtgc gccgagccgg ccgatcggat
     1081 gcagggaaac cagatgttgc cgcaccaatt cggcatgttc gccctgcatc gcccgttcga
     1141 ccatcggtgt caggatgtaa ccgggatgga ccgagttgac gcgaatgccg taacccgcct
     1201 tggcacagtg gagcgccgcc gacttggtga gcagtcgcac accgcccttg ctggcgttgt
     1261 aggcggccag attggggtcg ccgaccagcc cttcgatcga tgagatgttg atgatcgaac
     1321 caccccgccc cttcatcgcc cgaatggcat gcttggtgcc aagaaagacc gcatcgaggt
     1381 tgatgctcat caggtgacgc cactgctcga gcgtggcatc ttcggcattg ccgccgacgc
     1441 cgatgccggc attgttgacg accacatcga gcctgccgaa gcggccgagc gtcgcggaca
     1501 ttaccgcctc ccactctgat tcattgcgga catcctggcg cagaaactgc gcctcgccac
     1561 cggcggcaac gatctcgtcc gccaccgcct gaccatcgcc ctcgttgaga tcggtcacca
     1621 ccaccttggc cccctgctgg gccagcagca acgcctgcgc cttgccgagc cccgaggcgc
     1681 cgccggaaat cagcgccacc ttgcctgcca ctcgattcat tctgctcact cctgttggtt
     1741 gtcatggtcc tgtcggacaa cgcgcccagc ataacgcagc ggtccgcttt cttttacaat
     1801 cggaccggtt tcgatcgaac aacctctatc atccaaggag agcgaccatg tcgggcacga
     1861 tcatcgaatt cagcaccgcg gccggcggca actgccaagg ttatctgagc attcccgcag
     1921 ccggtaaagg cccggcagtc atcgtcattc aggagtggtg ggggttggtc gaccacatca
     1981 aacaggtggc cgaccgtttc gccgagaccg gctttgtcgc actggcaccc gatctctacc
     2041 atggcgtcca agcccgccag cccgacgaag ccggacggct gatgatggcg ctcaacatcg
     2101 acggggccgc cagcgacctg agcggcgcgg ccgattttct gctcgaacac cctgccgtca
     2161 ccagccgcaa ggtcggcact gtcggtttct gtctgggcgg ccagctctcg ctgatggccg
     2221 cctgtgtcga cacgcggatt ggtgcctgtg tcgactatta cggcgttcat ccgcagattc
     2281 agcccgattt cagcaagttg gcgagcccgg tgctcggctt tttcggcggt ctggatggct
     2341 ttgtcacacc cgacgcggtc agcgcactcg agcagcagat ccgccaagcc ggcaagtcgg
     2401 tggagtttca tgtctatccg gatgccgatc atgccttctt caatgacagc cggcccgagg
     2461 tctaccaccg ggcccatgcc gaagagagct ggaagcggat gctggcgttc tttcacgcca
     2521 acctgtaacg ctagggcgcg gaggagtagc tgttgcgaaa acgccaggtg cgaatgatct
     2581 gcaacacatc ggtttcgcgg ctcagctcgg gtggaaaggg ggcaaagggc tcggccagcc
     2641 ggacgatgcg caccgccgcc tcatcgagca gcggatggcc ggaggattca atgatctgga
     2701 ttcgatcgac tgagccatcg ggcagcagcc gcaccatcat gcgcaaactg ccataaatct
     2761 gctgatcccg cgcctgctgc ggatagttga ggttgccgac ccgctcgatc ttttgccgcc
     2821 aggcttcgag gtagtaggca tcgaccgacg actgggtcga ggcggcgctg atggtacgca
     2881 cccgcggccg acgtgccttg acctgctgct ggcggtcgag ctctgcctcc agactggcaa
     2941 tctcgatgct gcgctcgagc agggtcgaat cctgcgccgg agcggccacc tcgggcgtcg
     3001 gttcggcgcg ttgcggcacg ggcttggcag tctggctggc gcgggacacc gccggcggta
     3061 cactcctggg cctgggctgt ggccgtgctg gcgcctgttc gctctggctc agctgctgtc
     3121 gcggactctg ggtggtggct ggtttggggc tggtgaggcg ctgtgcctgc tcagcctcac
     3181 cactgccctg ttgatgcacc ggcgcgatga agtcggcctt gggcggtggc ctggcgctcg
     3241 cctgcgtcgc cagtgtcacc tcgatggtcg gctggctctc gggcggtggc ttgaggttgg
     3301 ccttgaagct gatccccagc agcaacacac catgaatcgc cagcgccaga aagagcgtga
     3361 aacccaaacg atcgagatcg gagaccgccg cccccctcac cgctggcgaa tcagccacgg
     3421 cagacaatgt cagcgtccga gccgccgggc gattgcatcc atcaactgtc cggcgatgtc
     3481 gatcgagaac tggttctgca attcgcgaat gcaggtgggg ctggtgacgt tgatctcggt
     3541 caggcagtca ccgatcacat cgatgccgac gaagatcaag ccccgctccc gcagggcggg
     3601 accaagctgg gcgcaaatcc agcggtcacg gctggtcaag ggtcggcctt cgccagtccc
     3661 gccggccgca aggttgccac ggctctcacc cggtgccgga atgcgcgcca gcgcgtaggg
     3721 caccggctcg ccatcgatca gcaggatgcg tttgtcgcca ttgcgaatct cgggcaggta
     3781 gcgctgcgcc atgatgtaac gctgaccatg gtgggtcagg gtttcgagaa tgaccgagac
     3841 attgggatcc tcgggcgtca gccgaaagac cgatgcgcca cccatcccct ccagcggctt
     3901 gacgatgatg tcgccatggt ggcggtggaa atcgcgaatc tgccccgcct gccggctgac
     3961 cagcgtcggc ggacccagtt cggggaaggt ggcggcaaac atcttctcat tgcagtcgcg
     4021 cagcgactgc ggccggttga ccaccagcac cccttcgcgc tcggcctgtt cgagcaggta
     4081 ggtggtgtag atgtattcgg tgtcgaacgg cggatccttg cgcatcagga tcgcatcgaa
     4141 gcgcgaaagc gggccctgtt gcggctcacc gcggctgaac cagcgctgcg ggtcgcgaaa
     4201 cacctcgagc ggacggctgg acgccattgc ccgcccttcg gtcagacaga ggtctttctg
     4261 ctccatgtag tgcagccgcc agccgcgctc ctgtgctgcc cacagcatgg ccagggtgct
     4321 gtctttcttg tagctgacgg cctcgatcgg gtccatgacc acgccaatct caaggggttt
     4381 gggcatgctg ctctcagtga aaatgaaaat gaagttgaac agggaagaaa aggcacgcgg
     4441 ttaacttacc cggccagctc cattgcagca agaggcgcag tgtcgcgcca tctccgccgg
     4501 aaagtgcgat ccaggtcgct cttctgcaca ctgccgctgg cgacaccagg gggcagcgct
     4561 aggattaact ctgagggcga tgacagggcg cccgatcacg aactctccgc agtcaaatcg
     4621 agtgcacgcc aatggatgaa agcaattttt ctggcctcaa ggtgatggtg atcgacgaca
     4681 gcaagaccat ccgccgcacc gccgaaagcc tgctggccaa ggtgggttgc gaggtgatca
     4741 ccgccaccga cggcttcgaa gcgctggcga aaatcgccga cggccgcccc gacatcatct
     4801 ttgtcgatgt gatgatgccg cgtctggatg gctatcagac ctgcgcgctg atcaagaaca
     4861 accgcgagtt ccgcgccacg ccggtgatca tgctgtcgag caaggatggc ctgttcgaca
     4921 aggccaaggg ccgcatcgtc ggctcggatg agtacctgac caagcccttc agcaaggagg
     4981 agctgatggg tgcaatcgaa caacacgtcg tgcggcactg aaggagcgca acatgggtgc
     5041 tttggcttat cgtccctcgg gcaatgacca gtcggccatc gcagcgcggg ttctgatcgt
     5101 cgacgactct cccaccgaaa ctctggccat ctcgaccatg ctgcggcggc acggctatca
     5161 ggtcttggcc gccacctccg gcgaggaggg catcgagact gcccgccgtg agcagcccga
     5221 tctggtgctg atggatgtgg tgatgccggg aatcagcggt tttcaggcca cccgtcagct
     5281 gacgcgtgaa caggcaaccg gccacattcc ggtggtgatc atcagcacca aggaccaacc
     5341 caccgaccgg gtgtggggat tgcgtcaggg tgcgcgggag tatctctgca agccggtgct
     5401 cgaacagcgg ctgttgcaga ccatcgagca gattctgacc cattgatgac aggagcaggc
     5461 gccatgcatc cacccgccga tccagccgat ctgctgctcg acatcgagcg gcgctgccag
     5521 cgacatgccg ccgcactgcc gcagccgacc cgcccggaac gcaccatcga caccggcatc
     5581 ggttttctgt tcgccggcct cgaaatggtg gcggccatga gtgagatcag cgaaattctg
     5641 gcggtgccac gcctctccaa ggtgcctggt gtcaagccct gggtctgcgg cattgccaat
     5701 ctgcgcggcc ggctgttgcc gatctatgac atcgatctgc tctgtggtca cgaacgcgat
     5761 cgcacggtgc agcaacgcga acagcgcgtg ttggtgaccg aatgcagtgg ccaatacagc
     5821 ggtttcatgg tcagcaggat cttcggcatg aaaccgatgg atccggaccg cttcgaatcg
     5881 aacacgcccg ccgcacccag cgtgctctcc tcctgcattg aagggtttta ccgcaatggt
     5941 gaacagacgt ggctgcgcct gagtctgcac cggctggccg ctgcaccagc cctgcaacag
     6001 ctcgcccgct gagcggtcgc cctcctcttc cacgcaccgc gcgcccgtca ctcttgaact
     6061 agactcgatt catgatcaac ccgttcccac cagcgagcat cgtcatgtcc aattcagcct
     6121 ccaaccccat ctcctctgga tcgacgtgga cggtgacggc gccactgctg ctctgcgccc
     6181 tgtcgatcgt gctgctgctg ggcaacaccc tgttgccgga aagcccgatt ccaccgctcg
     6241 gcagtctgct gctcggcgca ggcggcctgc tgacgctgct ctgggcagcg ctggcgatgc
     6301 ggcgcgcgca cagccggcaa ctgcaacaca gtgccgccat cgaacagcag aatcaggagg
     6361 ccatcctgcg tctgctggat gaactcgaag ggctgtcgcg gggtgatctg accacccagg
     6421 tcacggtctc cgaagccttt accggcgcca tcgccgattc gatcaacttc accatcgagc
     6481 agctgcggca actggtcatt tcgattcatg ccgcctccag ggagatcctg cagggcagcg
     6541 aaaactccca cgccaccgcc ctgcaactgg ccgaggctgc ccagcgacag gccagcgaga
     6601 tcggcacggc ttcggcctcg atcagccaga tcgccaccag catcgaacag gtctcgagcc
     6661 atgccgccga atcgagtcag gtggcggaac gttcggtcac catcgccaac tctggtgccg
     6721 aggcggtgac ccgcaccatc catggcatga cgatgatccg cgagcagatt cagagctcgg
     6781 ccaaacgcgt caagcggctg ggcgaaagtt cgcaggagat cggtgatttt ctggcactga
     6841 tcaacgacat cgccgatcag accaacatcc ttgccctcaa cgccgccatt caggcatcga
     6901 tggccggcga agcgggccgc ggctttgccg tggtcgccga cgaggtgcag cggctggcgg
     6961 agcgttccag cgccgccacc cgccagatcg aggccttggt ccggaccatc cagagcgaca
     7021 ccaacgaagc ggtgctgtcg atggaagaga ccaccagcga ggtggtgcgg ggcagtcaat
     7081 tggcccagga ggcgggcgtg gcgctggacg gcatcaagga ggtgtcagcc agtctggcac
     7141 aactgatcag tcacatctcc agcgcggccc agaatcaatc tggttccgcg gccgaagtgg
     7201 ccagaaccat gggatcgatt caggagatca ccacccagac cgcggccggt gctgctgcgt
     7261 cgggcaatgc agtcggcgac ctctcggcac tggcccaccg tctgcgccag tcggtggatg
     7321 gcttcaccct gcctgccgcc ccgtccaacc gctgatgagg agcgcctggt gaacatcgcc
     7381 tcttcacagc ccggcgtcac ctccagtctc atggcggcgc gctggctgct gccagagatt
     7441 caggtcgaac tccatgcgat ccaggtactg ctcgatcagg cagccaccga ttcgcagagc
     7501 gaaagtgagg tggccgaccg cctgcgccaa ctcggcaacc tgctcaccgt cgccgaactg
     7561 acactgccgg ccacgctctg ccatgagctg cgtcaggcgt tgcagacgac acgattttca
     7621 ctccctgccc gcgagctggc actggagatt ctgctgcggc tgccggatct gattggtgag
     7681 ctggcccgcg atgagcctga cgccctcgcc ctcacctgga ttctgcgcgc cgagctgcaa
     7741 cggttgctcg gccgccccct tccggaacca ctctcccggt cgatcggacc gccagcggcc
     7801 gtggcactct cggctgaaca gctcgctcac tacccagccg aacgctttct gccactggcg
     7861 gccaaacttc acgccaccta ccgcgatggt ctgctccaat accggcagac gaacatggcc
     7921 gctgcactgc agggcatgca gcgggtggtg ggcaaactgc tcgaaatcgc accacaaagt
     7981 ccctccagtc cgctgttgca ggccctgttg cggatccttg cagcgatgga gcaggcacgg
     8041 ctcgggccgc gcgctgccct gctcgatctg ctgagcgaat ttgagccatg gttggcccga
     8101 ctcgtcacga ccggcgcggc gctgctcaat cagccaccca cgctgttggc ggcactgcaa
     8161 ccctatgccc agttggccgg accgatggat gggcatgacg acaccacttc gcccttgctg
     8221 ccgatcagcg gtaccggagc cgcacgcgag gtgttgctca atgagctggc catcgaaatc
     8281 gaccacctca agacccgact cgaagagaat ctcgatctgc ccgcactgca ccaggagtgt
     8341 gagcggctgc gatcgaccat ccggctgctc gccctgcccg atctgacccg agccatgcag
     8401 cgggtgatcg aactgctctc ggcaacgccg gaacggctcg accgactgca actggccgaa
     8461 gcagtgctcg aactcgaagc ggcagtgatg aacccggaca gtcaggtcgc cgccacgacg
     8521 caacggaaca gcggactgcg tcaggcacaa cagcagttca tgcagcagtg cgcgcaggag
     8581 ctggaagagc tgcgccaacg gctcgatgac catctgaccc tgccggacga taaccctttg
     8641 ccgccacagg tcgagcaggg gcttcagcag ctgcaggggg gcttcaacct gatggagtgg
     8701 ccggaactgg ctacggccat cggtgtcgcc cggacacaat tgctcgctcc ccagcggcca
     8761 gcgcccgcgg atttggcact gatcgtcgac ctgctgatcg gcaccgagtt ctatcttcag
     8821 cagtggctgg ccggcgaacg cgacgctcca tccgccaacc tgatcaatct gtgcaaccgc
     8881 ctcatccgcg ccccacagga ggagagccaa gccacccctg ccccggcgcc cgccgaggat
     8941 gccagcgaca gcggcttcga tgccgagatc gcggccgtct tcgtcgaaga ggcgcaggag
     9001 gtgctggcag cactggatga acagtggccc gccttcgccg caggcgcgca gggtgccctc
     9061 atcgaggtgc gccgcggctt ccacaccctg aagggcagtg gccggatggt ggccgccgat
     9121 acggttgcgg cactggcctg ggccgtcgag cagcgcctca accagctgat cgatctgggc
     9181 catggcctca gcggtgaatt gatcgaactg atcgatcaga cccgcaatct gctgccggaa
     9241 ctgctcgact gttttgccaa cggacaggcc gccgcacccg gctggcaaca gctgctgcaa
     9301 cggatcgaag cctctgacaa tgccgaggag acgaagccag aaccgcaggc tcggccagag
     9361 tcggccacgg ccactgcaac agcagccgaa gagagtctcg atgcggtctt cagccgcgaa
     9421 gcggcactcc atcttcagcg aatcgaacag attctggccc atcccgacag tccgcagggc
     9481 gactggcgac tgctggaacg cacgctgcac accctcaagg gcagcgccaa tgccgcgggc
     9541 cgccggccac tggcccaact ggctggcgcg atgctcgatg cggtacagcg ccatctgcag
     9601 gagggtgagg cggcaccgac cctcgcctgc cgtgccggcg cggagctgct gccgatgctg
     9661 gtgctccatg cgggcgatga cgcaaccctg ccaccgcaat tccatgcggc agtcgaattg
     9721 ctgcagcaac cgctttgcga accagaggcg ttgtccaccc gctggctcga cgctgccgac
     9781 acgctggtgg cagcactcga gcagctcggc agcgctccgc agcaagcaca ggcgcggcag
     9841 cagatcgagc aggcaatcag cgccgccctg ggtgaacagt tgcccgcggc catccgcaac
     9901 tggatcgacc acctccatct ctggatggcg cagcacttcc atgccgaccg catcgaagcc
     9961 cgcgcccggc tggcactgtg ccgccaggcc ctgatcgaat cgatcgaagc cgaggtgctg
    10021 aatcagcccg ccagcgactg gagtgagctg tggcagccac tgccggagcc tcaacgacca
    10081 ccctcgaccc cggagcgcgc cttcgccacg ccccaaccag aggagaaaag tgcggctgcc
    10141 gcgcccatgt cggcagccac ccgcgaagcg gatgaagagc tgctggcaat tttcatgggc
    10201 gaggccgaag agctgctcga ggagattcag gcgattcagc gccactggcc cgatcggccg
    10261 gccgccacgt cggaacaggt tgcggcattg gcgcgggcgc tgcatacact caagggcgga
    10321 gcccgtctgg tgggtcagcg catgctggcc gacatctgtc atgagtttga aacccggctc
    10381 gactcgctcc gcgtcgctga ggtcggtcct gacgaactga gtggactggt caggcaactg
    10441 caagcagtga tttcggccga gcagtcgaca gatgtcgaca tcgactcccc ctccacccgg
    10501 ctcgaaacac ctgaggtcgc gctctcagta ctgcctcctg agacatcgac cgtccaagcc
    10561 actggagccg aaccggccga gatcaccgat ccaatcagga gtccggcacg cggcgagacg
    10621 ctgcggctgt cggccagtct gctgtcacac ctgctcgatc tgaccaatga atcgaccctg
    10681 ctgcaggagc ggctcgaacg gcagagccgg gagttcggcc aggccgtcga tgaaatggag
    10741 ctgaccatag accggctgcg cgaccaggtg cgacggctgg acatcgaggc cgaaagcgaa
    10801 ctccgactgc gccaccgcca aggcagcagc gagcgacagg atttcgatcc gctggagctc
    10861 gaccgctatt cacagttgca gcagttgagc cgtggactcg tggagagcgc ctccgacatc
    10921 aaggatctgc gtgacgccat ggccggccgc ctgcaacaga tgcgtggtca attgttgcag
    10981 cagtcgcacc tgcacaccga actgcgcgaa gggctggcgc gggcgcagat ggtgcccttc
    11041 tcgcggctgg ttccacgact tggcaagatg gtgcgccagt tggccaccga attgggcaag
    11101 gatgtcggac tgcgggtgat caaccccgag gtcgagatcg accgcacggt gctcgaacgg
    11161 ctgatcacac cgctggagca catgctgcgc aatgcgctgg accatggcat cgaaagcccg
    11221 gcgcaacgcc tggcactggg caagagcgcc cgcgccagca tcactctcca actgcaaccg
    11281 cagggcagca atctgctgct gagcgtcagc gacgatggtg ccggcatcaa cgtcgaacag
    11341 gtgctccaca aggcgcgcag tcgcgggctg atcgaccctc aacggcaatt gagtccgaac
    11401 gaagcactgc aactgatctt cctgcccggt ttctcgactg cctctgtcgt cacgcagatc
    11461 tccggtcgcg gggtcggcat ggatgtggtg cgcaatgagg tccaggcgct gggtggcacc
    11521 attgacgcca gcagtcgccc cggcgaaggc acctgctttg aactgcggct gccctacaca
    11581 ctgtcagcca atcgggcctt gatggtgcgc gtgggcggtg agctctatgc gctgccgctg
    11641 gagaccatcg aaggggtggt gcgggtcagc ccctacgagc tcgagcacca ctatcagcaa
    11701 cccgacagcc gctatgccta cgccgggcgt gactaccggc tgcgccacct tggcacgctg
    11761 ctgttcggct ggaatccgcc gacactggac ggcgtcaccc gcccgctgcc ggtgatcctg
    11821 ttgcgcagtc aggatgatgc ggtggcgttg caggtcgatg cgctcgaagg cagcagcgaa
    11881 attgtcgcca aggcgctcgc gccgcagttc aatcaggttc cgggcattgc cggtgcctcc
    11941 ttgcagggcg atgagggcgc ggtgatcatt ctcgacctgc ccgccctgct gcgcgccaag
    12001 cctg
//
LOCUS       DAORCQ010000071        11902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3554_length_11902_cov_6.375032, whole genome shotgun sequence.
ACCESSION   DAORCQ010000071 DAORCQ010000000
VERSION     DAORCQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3554_length_11902_cov_6.375032"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..2580)
                     /gene="gyrB"
                     /locus_tag="PKI23_08255"
     CDS             complement(148..2580)
                     /gene="gyrB"
                     /locus_tag="PKI23_08255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNN36969.1"
                     /translation="MSDQAEENVYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHM
                     VFELVDNSIDEALAGYCSQVDVIIHPDESITVIDDGRGMPVDIHEEEGVSAAEVIMTV
                     LHAGGKFDSNSYKVSGGLHGVGVSVVNALSSTFRLRVRRDGKVHEQIYHDGAPEAPLH
                     VIGDTDRTGTECHFAASSEIFTNIHFSYDILARRLRELSFLNSGVSIRLKDERSGNSE
                     EFCYEGGLNAFLTYLNQHKSVLNRPLYFCKQRDDGIGVEVALQWNDSFQENILCYTNN
                     IPQRDGGTHLAGFRSALTRTLNQFIEQEGLAKKSKVTTVGDDVREGLTAIISVKVPDP
                     KFSSQTKEKLVSSEVKAAVEQEMARYLTDYLLEHPQDARSIVQKIIDAARAREAARKA
                     REMTRRKGPLDIAGLPGKLADCQEKDPALSELFLVEGDSAGGSAKQGRDRRFQAILPL
                     KGKILNVERARFDKMLSSQEVGTLITALGCSIGADEFNPEKLRYHRIIIMTDADVDGS
                     HIRTLLLTFFFRQMRELVERGHVYIAQPPLYKIKKGKQESYLKDEQALDGYLTQGALD
                     EALLVPATGIPPIQGVALEQLVNEYQSVKRTIERLSRLYPPTLLWALLEVPLLSLSAL
                     PAADQVQTWVEQLQGLLLHKGGESRNERWACQIAEDRERHLFCPQIDLTVHGTTQRYL
                     LDHDFLASGEYRAISALQVKLHGLFSDGGHVQRGERKQAVTDFGQALEWLIAESRRGA
                     TIQRYKGLGEMNPEQLWETTMDPAARRLLQVTIEDAIACDLMFNTLMGDEVEPRRLFI
                     ETNALKVSNLDV"
     gene            complement(2603..3751)
                     /gene="recF"
                     /locus_tag="PKI23_08260"
     CDS             complement(2603..3751)
                     /gene="recF"
                     /locus_tag="PKI23_08260"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNN36970.1"
                     /translation="MLLTRLQVTHLRNLTNLELEPAAGFNLIIGDNGSGKSSLLEAIH
                     LLGAGRSFRHTSSKANLTGLIQHGTEQALCVGRFLTTSDDVDDMSNLHPFSIGVALHR
                     RTGITAKAAGERVAAASHLARTLPLMVINSWTFQLIEGPSQLRRQYLDWGLFHVKQDY
                     LATLQGWRRCLRQRNQLLRTRHGKIAGELTLWSRELARHAEAVDAARRDYTAALTPWV
                     TSLSERLGAVPDLELTYQPGWDSAIPLLEQMERDLESDRARGFTAHGPHRADLRLKWN
                     GIAVAERLSRGQIKSVVLTLKLAQERMVRSSGSKRPVFLIDDLAAELDSSHRQLFCEL
                     LAEERPQAFLTALERDSIDLSWFGQAGSRLFHVEQGRLRECSVIDRER"
     gene            complement(3868..4998)
                     /gene="dnaN"
                     /locus_tag="PKI23_08265"
     CDS             complement(3868..4998)
                     /gene="dnaN"
                     /locus_tag="PKI23_08265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNN36971.1"
                     /translation="MKFAITREALLQPLQQVIGVVERRQTLPILANVMLEVNSGLLAV
                     TATDLEVELIGKIALEQPHEEGVTTVPARKLLDICRSLPDGASIEIETAEGRLHLRAG
                     RSRYRLATLAAEEFPNVDEGQAPALAAFAVAQSILKRLIEKTSFAMAQQDVRFYLCGM
                     LLEISGGAGQLRGVATDGHRLAMYTVENIESVATGLLSNRVQAIIPRKGTLEIARLLK
                     ESEEPVRVQLTQHHLRIENSDVVIISKLVDGKFPDYEKVIPRGGDKVLLGERLALRDA
                     FNRAAILSNEKYRGVRMQLDRDTLRLIASNPEQEQAEEELSVHYQGPQMEIGFNVGYL
                     TDALAALDQNEARFTFSSAESSVLIDVPNDASGCYVVMPMKL"
     gene            complement(5057..6460)
                     /gene="dnaA"
                     /locus_tag="PKI23_08270"
     CDS             complement(5057..6460)
                     /gene="dnaA"
                     /locus_tag="PKI23_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNN36972.1"
                     /translation="MVAAWTRCADQLQRELSAQQYNTWIRPLQACGRDEARELCLLAP
                     NRFVLDWVSEKFLDRIRELLREGCGGTAWQVVLEIGSSEKPPQTAMNGQHQSIAPSQT
                     ATAITTSPRARRGDVIQAEGAVHHESGLNADFTFDTFIEGKSNQMARAAALQLAVSGG
                     GMDAYNPLFIYGGVGLGKTHLMHAVGIALLQKNPSARVVYLHSERFVADMVKALRLNA
                     INEFKRFYRSVDALLIDDIQFFAGKDRSQEEFFHTFNALLEGRKQMILSCDRYPKEIT
                     GLEDRLKSRFGWGLTVCIEPPELETRVAILLKKAEQMGVQVGHDVAFFIAQRIRSNVR
                     ELEGCLKRVVASARFTGRPIDLELVKEALKDLLALQERQISIDNIQSTVAEYYKIKRS
                     DLLSKRRNRSVARPRQVAMALAKELTNRSLPEIGEAFGGRDHTTVLHGCRKIKELCNS
                     SADIAEDYKNLLRLLTV"
     gene            6799..7275
                     /locus_tag="PKI23_08275"
     CDS             6799..7275
                     /locus_tag="PKI23_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNN36973.1"
                     /translation="MLLPATPLCSPPSERVAGYNKAINGQQEQTAEDDPMINYITLGT
                     NDLERAAKFYDELLALVGGKRAMTSERSVGWVGPKGAPMLMIMKPFDGKPATAGNGTM
                     VALSADSPEQVAALHAKALALGGSDEGAPGPRGDSFHCGYFRDLDGNKWNAFYRNR"
     gene            7482..7616
                     /gene="rpmH"
                     /locus_tag="PKI23_08280"
     CDS             7482..7616
                     /gene="rpmH"
                     /locus_tag="PKI23_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNN36974.1"
                     /translation="MKRTFQPSVLKRKRTHGFRARMATKNGRQVIQRRRAKGRKRLSA
                     "
     gene            7609..8010
                     /gene="rnpA"
                     /locus_tag="PKI23_08285"
     CDS             7609..8010
                     /gene="rnpA"
                     /locus_tag="PKI23_08285"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNN36975.1"
                     /translation="MPDQPPAAERAFGFSRPLRLLKAGEFDAVFKHNVQRASSRELLL
                     LARPNDLGHPRLGLVVAKRQVRRSVDRNRLKRVLRETFRQQQHALGSHDIVALVRAPF
                     QELPSEQHHRFAEQQWLRLLKRLHSSPELDR"
     gene            8012..8257
                     /gene="yidD"
                     /locus_tag="PKI23_08290"
     CDS             8012..8257
                     /gene="yidD"
                     /locus_tag="PKI23_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNN36976.1"
                     /translation="MQTVILGLIRLYRYCISPLLGPNCRFHPSCSSYAEEAITRFGPL
                     RGGWLALKRIGRCHPWHVGGHDPVPHSLDPSNRSRTS"
     gene            8254..9945
                     /gene="yidC"
                     /locus_tag="PKI23_08295"
     CDS             8254..9945
                     /gene="yidC"
                     /locus_tag="PKI23_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007948101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNN36977.1"
                     /translation="MNNLPRYLILAALAVVGYLLLNAWRNDYVQPAASVPEITQPQNQ
                     GTDVGAVEQPTSKVANAELPQSVPKAAVPLATVPEKNGKNSGLIEVKSDLLTLKIDPT
                     GGDIVAAALNRYPARIETPDVPFSLLARNGRTYVAQSGLIGPDGPDASPEGRPLYSSE
                     HSSYQLAEGQDQLIFDLRHQSASGVVITKRFTLTRGSYLIDVRYLIDNSSQQPWQGNF
                     FAQLKRDNSPDPSAGNQGFGVNPYLGTALYTPDKPYTKVPFDQIAKEPLSLEIKGGWV
                     SMVQHYFISAWVADPDSSFLYETRKLGGKEQYIIGFKQPTELSVAPGQSASVGARLYI
                     GPKIQKSLKTIAPGLDLTVDYGWLWFIAQPLFWILDWIHGLVQNWGVAIILLTVLVKA
                     AFFHLSATSYRSMAKMRNLQPKMLELRDRHGNDRQQLSQATMELYRKEKVNPLGGCLP
                     ILVQMPVFIALYWTLLESVELRHASFLWLNDLSVMDPYFVLPIVMGVTMFIQQLLSPA
                     PPDPMQAKVMRLMPVMFTFFFLWFPSGLVLYWLVNNLLSIAQQWFITRQIEAAAN"
     gene            9961..11358
                     /gene="mnmE"
                     /locus_tag="PKI23_08300"
     CDS             9961..11358
                     /gene="mnmE"
                     /locus_tag="PKI23_08300"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNN36978.1"
                     /translation="MSVIGSIDRDTIVAIATPPGRGGIGILRCSGPLSLSIAEAILGK
                     RPTARHAHLADFLDEQGRLIDQGIALYFPAPHSFTGEEVLELQGHGGPVVLDLLLQRA
                     LRLGARLARPGEFSERAFLNDKIDLLQAEAIADLIDSSSVAAARSALQSLQGEFSRQI
                     NQLVERLIRLRVYIEASIDFPDEELDLLADPQIGQQLLDVSNHFRAILSKAKDGQRLR
                     DGMSVVIIGRPNAGKSSLLNQLAEHDRAIVTDIPGTTRDLLHEEITLQGVPLHLTDTA
                     GLRQASDPIEQEGIRRARQQADQADCVLLLVDATADRSHWLEPIDADRNDPLWQRLTL
                     VRNKIDLLDEVPKMTQEAFFAEGERSLTLPVIALSAKTAAGIDLLKQHLLERIQFRPS
                     EEGGFLARRRHLDALLRAQALLEQGQRAFAATGQGELLAEDLREAQQQLDEITGRFGS
                     EALLSRIFSSFCIGK"
     gene            complement(11559..>11902)
                     /locus_tag="PKI23_08305"
     CDS             complement(11559..>11902)
                     /locus_tag="PKI23_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN36979.1"
                     /translation="VITTATEIAISAADCVKNSAETVITTVLDQDGDGKLDQEDLRIL
                     TQKGTEIGKVMGTKAALLASEAAKSEIVKDAVAAAAVGAAIGVALPLVGPAAGAVVGA
                     TLGAHKNLTKK"
BASE COUNT         2286 a         3733 c         3451 g         2432 t
ORIGIN      
        1 tgcagaattg gggacagacc atggttttcc ccctgtttag cactggctag cagaaagccg
       61 gaaaaccgtg gtctatcctc agctctgcca attctgcaag cgacaattca acgagctcat
      121 cgaaaataca ttttttcaat gaatgaacta cacatccagg ttcgacacct tgagtgcgtt
      181 ggtctcgatg aagagccggc gtggctcgac ctcatccccc atcagcgtgt tgaacatcag
      241 gtcacaggcg atcgcatcct cgatcgtcac ctgcagcaat cggcgcgcgg ccggatccat
      301 cgtggtctcc cacagctgct ccgggttcat ctcgccgagt cccttgtaac gctggatggt
      361 tgcaccgcgc cgtgactcgg caatcagcca ctccagggcc tgaccgaaat cggtgaccgc
      421 ctgtttgcgc tcgccgcgct gcacatggcc gccatcgctg aacaggccat gcagtttcac
      481 ctgcaatgca ctgatcgcgc ggtactcgcc cgacgccaga aagtcgtggt cgagcagata
      541 gcgctgggtg gtgccatgca cggtcaggtc gatctgcggg cagaagagat gccgctcacg
      601 gtcttcagcg atctggcacg cccaacgctc gttgcggctt tcgccaccct tgtggagcaa
      661 caggccctgc aactgctcga cccatgtctg cacctgatct gccgcaggca atgcggagag
      721 cgacagcagc ggcacttcga gcagcgccca cagcagcgtg ggcgggtaga gccgtgacaa
      781 ccgctcgatg gtccgcttga cgctctggta ctcattgacc agctgttcga gcgccacgcc
      841 ctgaatcggt ggtataccgg ttgccggcac cagcagtgcc tcgtccagcg cgccttgggt
      901 caggtagcca tcgagcgcct gctcatcctt cagatagctc tcctgcttgc ccttcttgat
      961 cttgtacagc ggtggctggg cgatgtagac atggccgcgc tcgaccagtt cgcgcatctg
     1021 ccggaagaag aaagtcagca gcagggtgcg gatgtgcgaa ccatccacat cggcatcggt
     1081 catgatgatg atgcggtggt agcgcaactt ctccgggttg aattcatcgg cgccaatgct
     1141 gcatccaagc gccgtgatca gggttccaac ctcttgagaa gagagcattt tgtcaaagcg
     1201 ggcccgctcg acattgagga tcttgccttt cagcggcaga atcgcctgga acctgcggtc
     1261 gcgcccctgt ttggctgacc ctccggcgga atcgccctcg accagaaaga gttcagagag
     1321 cgccggatcc ttctcctgac agtccgccag ctttccgggc aggccagcga tgtccagcgg
     1381 ccccttgcgg cgggtcatct cgcgcgcctt gcgtgcggcc tctcgcgccc gcgcggcatc
     1441 gatgatcttc tgcacgatcg accgggcatc ctgcggatgc tccagcaggt agtcggtcag
     1501 gtagcgggcc atctcctgct cgaccgcagc tttcacctca gaggagacca gcttctcctt
     1561 ggtttgcgat gagaacttgg gatctggcac cttgaccgag atgatcgcgg tcagcccttc
     1621 acggacatcg tcacccaccg tggtcacctt gctcttcttc gccagcccct cctgttcgat
     1681 gaactggttg agcgtgcggg tcagggcgct gcggaaaccg gccaggtggg tgccgccatc
     1741 gcgctgcgga atgttgttgg tgtagcagag gatgttctcc tgaaagctgt cgttccactg
     1801 cagcgccacc tcgacgccga tgccatcatc gcgctgcttg cagaagtaga gcgggcggtt
     1861 cagcacgctt ttgtgctggt tcaggtaggt caggaaggca ttcaaacccc cttcataaca
     1921 gaactcctcg gagtttccgc tgcgctcatc cttcagccgg atcgacacgc cggagttcag
     1981 aaacgacagc tcgcgcagcc gtcgggccag aatgtcatag gagaagtgga tattggtgaa
     2041 gatctcgctg gaagcggcaa agtgacactc ggttccggtg cggtcggtgt cgccaatcac
     2101 atgcagcggt gcttcgggcg caccgtcgtg gtagatctgc tcatggacct tgccatcgcg
     2161 gcgaacgcgc agccgaaagg tcgatgagag cgcattgacc accgaaacgc ccacgccatg
     2221 cagaccgccc gaaaccttgt agctgttgct gtcaaacttg ccgcccgcat gcagtacggt
     2281 catgatcacc tcggcagccg acaccccctc ctcctcatgg atatcgaccg gcatcccgcg
     2341 accatcgtcg atcaccgtga tcgattcgtc gggatggatg atcacatcga cctgcgaaca
     2401 gtagccggcg agcgcctcat cgatcgagtt gtcgaccagt tcgaacacca tgtggtgcag
     2461 accactgcca tcgtcggtat cgccgatgta cataccgggc cgcttgcgga cggcgtccaa
     2521 ccctttcaac accttgatac tggaagagtc atagacattc tcttccgctt gatcgctcat
     2581 cattttctcc aaaaagaggt ttctaccgtt cgcggtcaat cacactgcat tcacgaagtc
     2641 ggccctgttc cacgtggaac aggcgagacc ctgcctgacc aaaccagctc agatcgatac
     2701 tgtcccgttc cagggcggtc aaaaatgcct gcggtcgttc ctctgccagc agttcacaaa
     2761 acagctgccg gtggctgctg tcgagctctg ctgccaggtc gtcgatcaaa aaaacgggcc
     2821 gcttgctgcc gctgctgcgc accatccgct cctgcgccag cttgagcgtc agcaccacgc
     2881 tcttgatctg ccccctggag agtcgttccg ccaccgcgat cccgttccat ttgagtcgca
     2941 aatcggcgcg gtgcggtccg tgggcggtga aaccacgcgc acggtcgctc tccagatcac
     3001 gctccatctg ttcaagcagc ggaatggcgc tgtcccagcc tggctgatag gtcaactcaa
     3061 ggtcaggcac cgctccgagc cgttcggaga ggctggtcac ccagggtgtc agcgcggcgg
     3121 tgtagtctcg ccgcgcggcg tccaccgcct cggcatggcg cgccagctct cggctccaca
     3181 gcgtcagttc gcccgcaatt ttaccatgcc gtgtccgcaa taactgattg cgttgccgca
     3241 ggcagcggcg ccagccttgc agagtggcaa ggtaatcctg tttcacgtga aacagacccc
     3301 agtcgaggta ttgcctgcgc aattggctgg gcccttcgat cagttgaaac gtccaggagt
     3361 tgatcaccat caatggcagc gttcgcgcaa gatgtgaagc cgccgcaacc cgctcgcctg
     3421 ccgccttggc ggtgatgcca gtgcgccgat gcagcgctac accaatggaa aaaggatgga
     3481 gatttgacat gtcgtcgaca tcatctgaag tggtcagaaa gcgtccgacg cagagggcct
     3541 gctctgtgcc atgctggatc aggcccgtga gattggcctt gctgctggtg tgacggaagc
     3601 tgcgtccagc gccgagaagg tgaatggcct cgagcaagga gctcttgcca ctgccgttgt
     3661 caccgatgat caaattgaag ccggcggcag gctcaagctc caaattggtg aggttgcgca
     3721 gatgggtcac ctggagccgg gtcagcaaca tggcatcagg ccaggagcag ccgccatccg
     3781 gcgaaaaaag gtggccgaaa cgggtggtta tgcctcaagt gccgggactt gcatcgccag
     3841 atccagaaga gggaggacgg catccgctca cagcttcatc ggcatcacga catagcagcc
     3901 gctggcatcg ttcggcacat cgatcagaac gctgctctcc gcggagctga aggtgaagcg
     3961 ggcttcattc tgatcgagtg cagccaaggc gtcggtcagg tagccgacgt tgaagccaat
     4021 ctccatctgt gggccctggt aatggaccga aagctcctct tcggcctgct cctgttccgg
     4081 attgctggca atcaaccgca acgtgtcgcg gtcgagttgc atgcgcacgc cgcgatattt
     4141 ttcgttcgac agaatcgccg cacggttgaa tgcatcgcgc agcgccagcc gctcacccag
     4201 tagcaccttg tctccaccgc gtggaatgac cttttcgtag tcgggaaact tgccatcgac
     4261 cagtttggag atgatcacga catcgctgtt ctcgatgcgc agatggtgct gggtcagttg
     4321 cacccgcacc ggctcctcgc tctctttgag cagccgggcg atttcgagag tgcccttgcg
     4381 cggaatgatg gcctgcaccc ggttcgacaa cagccccgta gccaccgatt cgatgttctc
     4441 cactgtgtac atcgccagcc gatggccatc ggttgccacc ccccgcaatt gtcccgcacc
     4501 gccgctgatc tccagcagca tgccgcagag atagaagcgg acatcctgtt gtgccatcgc
     4561 aaagctggtc ttctcgatca gccgcttgag gatgctctgt gccactgcaa aggcggccag
     4621 cgcaggagct tgtccctcat cgacattggg gaactcttcg gccgccagcg tcgccaggcg
     4681 ataacggctg cgcccggcac gcagatggag ccggccttct gcggtttcaa tctcgatgct
     4741 cgcgccatcg ggcagcgagc ggcagatgtc cagcaacttg cgtgccggga ccgtggtcac
     4801 tccctcttcg tggggctgct ccagcgcgat cttgccaatc agctccacct cgagatcggt
     4861 agccgtgacc gcgagaaggc cgctgttcac ctccagcatc acattggcca gaatcggcag
     4921 cgtctgccgc cgctcgacca cgccaatgac ctgttgaagt ggctgcagca gagcttcccg
     4981 cgttatcgcg aacttcatga tcttcctcgg ttttatgggt tgtgcagcca gtgatcccct
     5041 ggcggaatgg ctggtttcaa acggtgagca atcgtagcag gtttttgtaa tcttcagcga
     5101 tatcggcact gctgttgcac agttccttga tcttgcggca accatgcaac accgtggtgt
     5161 ggtcgcgtcc gccaaaggcc tcgccaattt ccggcaggct gcggttggtc agctccttgg
     5221 ccagcgccat ggccacctgc cgaggacgcg ccaccgagcg gttgcgccgt ttcgacagca
     5281 ggtcggagcg cttgatcttg tagtactcgg ccaccgtgct ctggatattg tcgatgctga
     5341 tctgtcgctc ctgcagggcc agcagatctt tcagcgcctc cttcaccagc tccaggtcga
     5401 tcggcctgcc ggtgaagcgt gcgctggcga ccacccgttt gaggcagcct tcgagctccc
     5461 ggacattgga acggatgcgc tgcgcgatga aaaaagcgac atcatgaccg acctggacgc
     5521 ccatctgctc cgccttcttc agcaggatgg ccacccgcgt ttccagttcg ggcggttcga
     5581 tgcagaccgt caacccccag ccgaaacgtg atttgagtcg atcctccagg ccggtgatct
     5641 ctttcggata gcggtcgcag gagagaatca tctgttttct tccctccagc agcgcgttga
     5701 aggtgtgaaa gaactcctcc tgcgaacggt ccttgccggc aaaaaactga atgtcatcga
     5761 tcagcagggc atccaccgac cgatagaagc gtttgaactc attgattgcg ttgagtctga
     5821 gcgccttcac catgtcggcg acaaagcgct cggaatgcag gtaaaccacg cgtgcggagg
     5881 gattcttctg cagcagtgcg atgccaaccg catgcatcag atgggtcttg cccagtccga
     5941 ctccgccata gatgaagagc ggattgtagg catccatccc gccgccgctg accgccagtt
     6001 gcagcgcggc cgcacgagcc atctgattgg atttgccctc gatgaaggtg tcgaaggtga
     6061 aatcggcatt caggccactt tcgtggtgca ccgccccttc ggcttgaatg acatcaccac
     6121 gtctggcgcg cggcgatgtc gtgattgctg ttgcagtctg cgatggagca atgctctggt
     6181 gctgcccatt catggcggtc tggggcggct tttcgctgct gccaatctcc agcaccacct
     6241 gccaggcggt gccgccgcaa ccttcgcgca gcagttcacg aatccgatcg aggaacttct
     6301 cgctcaccca atcaagcacg aaccggttgg gcgccagcag gcagagttct cttgcctcgt
     6361 cccgaccgca tgcctgcaat ggcctgatcc aggtgttgta ctgttgcgca ctcaactccc
     6421 gctggagttg gtcggcacat ctggtccatg ctgcaaccac ctggccccct gttctgtgtt
     6481 ctgatgttgc tgcgggagcg gcccatttta gtcagaccac caccgttaat ccacaaaaat
     6541 ccatgcgagc atgagaaata aaaactttgt tattcagtga attagaatat tcaagcgggc
     6601 ttgccaaaat ctttttgcgt ggattcacag agcagcttca caacctgttg ataacttgtt
     6661 catttcagtt gcatgacccg ctttgctgtt tgcggcaggc cgcatcagcc gtgagcgctt
     6721 tgttgccgag cggcgatttt tttcagcaca ggcagcagtt ttccatcatt tctttcgcca
     6781 gtgtgcggtc accgacttgt gctgttgccg gccacgcctc tatgctcgcc gccatccgag
     6841 cgagtcgctg gatataacaa ggccatcaac ggtcaacaag aacagactgc ggaggatgat
     6901 ccgatgatca attacattac cttgggcacg aatgacctgg agcgggcagc gaagttttac
     6961 gatgaactgc tggcgctggt cggtggcaaa cgcgccatga ccagtgagcg ctcggtcggt
     7021 tgggttgggc ccaagggcgc gccgatgctg atgatcatga agccattcga cggcaagccg
     7081 gccacggcgg ggaatggcac catggtggca ctgagcgctg actcaccgga gcaggtcgca
     7141 gcgctgcatg ccaaggcact ggcgttgggc ggcagtgatg agggcgcgcc gggcccgcgc
     7201 ggggacagtt ttcattgcgg ttatttccgt gatctcgatg gcaacaaatg gaatgcgttt
     7261 taccggaacc gctagaaagt ctttttcatc aagacagtgc gggttgaagg gagtgagtgc
     7321 cgctgtgccg ggcggcgggt gtggcgatgc cgtccgctgg agtggtctgc atcgccgcga
     7381 acccgccatg caattgccat ggggtggata ctcgcctaga atgccgccct ccttgcgaag
     7441 tcacgcttga catcgtccaa cgtcatctcc agatcaaggt catgaagaga acctttcaac
     7501 ccagcgtatt gaagcgcaag cgcacccatg gatttcgcgc cagaatggcc accaagaacg
     7561 ggcggcaggt catccagcgt cgccgtgcca aggggcgcaa gcgtctgagt gcctgatcaa
     7621 ccacccgctg cagagcgagc gttcggtttt tctcgcccgc tgcgcctgtt gaaggcaggt
     7681 gagtttgacg cggtattcaa gcacaatgtc cagcgggcct cgagccggga gcttctgctg
     7741 ctggcgcggc ccaatgacct gggccatccg cggctgggtc tggtggtggc caagcggcag
     7801 gttcgccgtt ctgtcgaccg aaatcgtcta aaaagagttc ttcgcgaaac cttcaggcag
     7861 caacaacatg cgctgggcag ccatgacatt gtcgcgctgg tgcgggctcc ctttcaggaa
     7921 ctcccgtcag agcagcatca tcggtttgcg gagcagcaat ggctgaggct cttgaagcgc
     7981 ctgcattcgt caccggaact cgatcgctga catgcaaaca gtgatcttgg gcctgatccg
     8041 gctttaccgc tactgcatca gtccgctgct cggtcccaac tgtcgttttc accccagctg
     8101 ttccagctac gccgaagagg cgatcacccg ctttggtccg ctgcgcggcg gctggctcgc
     8161 cctgaagcga atcggccgct gccatccctg gcacgtcggt gggcatgacc ctgtgcccca
     8221 ctcccttgac ccctcgaaca gatcccgaac gtcatgaaca acctcccgcg ctatctgatt
     8281 ctcgccgcgc tcgccgttgt cggttacctg ctgctcaatg cctggcgcaa tgactatgtg
     8341 caaccagccg cttccgtgcc cgagataaca cagccacaga atcaaggcac ggatgtcggc
     8401 gcggtggagc aaccgaccag caaggtcgcc aatgcggaat tgccgcagag cgtgcccaag
     8461 gcggccgtcc cgctggccac cgtcccggag aaaaatggca agaacagcgg tctgatcgag
     8521 gtgaagagcg acctgctgac gctgaagatc gaccccactg gcggtgacat cgttgccgcg
     8581 gcactcaacc gttacccggc gcggatcgaa acacccgatg tgccattcag cctgctggcg
     8641 cgcaatggcc gcacctatgt cgcgcagagt ggcctgattg gccccgatgg gcccgatgcc
     8701 agcccggagg gccggccgct ctacagcagc gagcacagca gctatcaact ggccgaaggg
     8761 caggatcaat tgatcttcga tctgcgtcac caaagcgcca gcggtgtcgt catcaccaaa
     8821 cgcttcacgc tgacgcgggg cagttacctg atcgatgttc gttacctgat cgacaattcg
     8881 tcgcagcagc cctggcaggg caacttcttc gcgcaactga agcgtgacaa cagccccgat
     8941 ccatcggccg ggaatcaggg tttcggtgtc aacccctacc tcggcactgc cctgtatacg
     9001 cccgacaagc cctacaccaa ggttccgttc gaccagatcg ccaaggagcc gctctcgctc
     9061 gaaatcaagg gtggctgggt ctcgatggtg cagcactact tcatctctgc ctgggtggcc
     9121 gatcccgaca gcagctttct ttacgagacc cgcaagctgg gcggcaagga gcagtacatc
     9181 atcggcttca agcagccgac cgaattgagc gtggcgccag ggcagagtgc cagcgttggt
     9241 gcacgcctct acatcggtcc caagatccag aaaagtctga aaaccatcgc gcccggcctt
     9301 gatctgaccg tcgactatgg ctggctctgg ttcattgccc agccgctgtt ctggattctg
     9361 gactggattc atgggttggt gcagaactgg ggtgtggcga tcatcctgct gaccgtgctg
     9421 gtcaaggcgg ccttctttca cctctcggcc accagctacc gctccatggc caagatgcgc
     9481 aatctgcaac ccaagatgtt ggagctgcgc gaccgccatg gcaacgaccg ccagcagctc
     9541 tcgcaggcca ccatggagct ctatcgcaag gagaaggtca atccactcgg gggctgtctg
     9601 ccgattctgg tgcagatgcc ggtcttcatc gccctctact ggaccctgct ggagagtgtc
     9661 gagctgcgcc atgccagctt cctgtggctg aatgacctgt cggtgatgga tccctacttc
     9721 gtcctgccga tcgtcatggg cgtcacgatg ttcatccagc aactgctgag cccggcgccg
     9781 cccgacccga tgcaggccaa ggtgatgcga ctgatgccgg tcatgttcac cttcttcttc
     9841 ctctggttcc cctcggggct ggtgctctac tggctggtca acaacctgct ctcgatcgcc
     9901 cagcagtggt tcatcacccg gcagatcgag gctgccgcca actgagggct cggtcgcgcc
     9961 atgtcggtca tcggcagcat cgatcgcgac accatcgtgg ccatcgccac cccgcccggt
    10021 cggggtggca tcggcatcct gcgctgctcc ggcccgctca gtctgagcat cgccgaggca
    10081 attctcggca agaggccaac cgcccgccat gcccacttgg ccgattttct cgatgagcag
    10141 ggtcggctca tcgaccaggg cattgccctc tactttcccg ctccccactc cttcaccggc
    10201 gaagaggtgc tggaattgca gggccatggc ggcccggtgg tgctcgacct gctgctgcaa
    10261 cgcgcgcttc ggctcggcgc ccgcctggcc cggcctggcg agttcagcga acgcgccttt
    10321 ctaaacgaca agatcgacct gctgcaggcc gaggcgattg ccgacctgat cgacagcagc
    10381 tcagtggcag cggcacgctc ggcgctgcaa tcgctgcagg gcgagttctc acggcagatc
    10441 aaccaactgg tcgaacggct gatccggctg cgggtctaca tcgaagcctc gatcgacttc
    10501 cccgatgaag agctcgacct gctggccgac ccgcagatcg gccagcagct tcttgatgtg
    10561 agcaatcact ttcgcgccat cctcagcaag gcgaaggatg gccagcggct gcgcgatggc
    10621 atgtcggtcg tcatcatcgg tcggcccaat gccggcaagt cgagcctgct caaccaactc
    10681 gccgagcatg accgcgccat cgtcaccgac atccccggta ccacccgcga cctgctgcac
    10741 gaagagatca cgctgcaagg ggtgccgctg catctcaccg acaccgccgg actgcggcag
    10801 gccagcgacc cgatcgagca ggagggaatc cgccgcgccc gccagcaggc cgaccaagcc
    10861 gattgcgtcc tgctgctggt cgatgccacc gccgaccgca gccactggct cgagccgatc
    10921 gacgccgatc gcaacgaccc gctctggcag cggctcaccc tcgtgcgcaa caagatcgat
    10981 ctgctggatg aagtgccaaa gatgacgcaa gaagcttttt ttgccgaagg tgagcgatca
    11041 ctcacattgc cggtcatcgc cctctcggcc aagacggcag ccggcatcga cctgctcaaa
    11101 cagcacctgc tggagcgcat ccagttccgg cccagcgaag agggcggctt tctggcgcgc
    11161 cgacgccacc tcgatgccct gttgcgggcg caggcgctgc tcgaacaggg ccaacgggcc
    11221 tttgccgcaa cgggtcaggg tgagctgttg gcggaagatc tgcgcgaagc ccagcagcag
    11281 ctcgatgaga tcaccggccg cttcggcagc gaggcactgc tttcaagaat tttttcatcg
    11341 ttttgcatcg gcaagtgaca tctgttccac cacgctgatc gagcgcgaca gcggctggct
    11401 ggggccatat tgctcggtgg agttgagcgc ggtgcgggcg aggcgcactt ttgttggagc
    11461 agccatccgt gatagagaaa aaagatcgct tggatcagct tcgtaaaacc gtggccctca
    11521 atgctgttcc tcttcatttt tcctgccaag aggaagcttc atttcttggt gagattcttg
    11581 tgggcgccaa gcgtcgcacc tacaactgcg cctgccgcag gaccaacaag cggcaaagcc
    11641 acgccaatcg cagcgcccac cgccgctgca gcgacagcgt ctttcacaat ctctgattta
    11701 gctgcctcgg atgccaggag agctgccttc gtacccatga ccttgccgat ctcggtgccc
    11761 ttttgagtga ggatacgaag gtcttcctga tcaagcttcc cgtcaccgtc ctgatccaag
    11821 acggtggtga tcacggtttc ggctgaattc tttacgcaat cagccgcaga tatagcgatc
    11881 tcagtggcag tggtaataac ag
//
LOCUS       DAORCQ010000072        11505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3721_length_11505_cov_6.671441, whole genome shotgun sequence.
ACCESSION   DAORCQ010000072 DAORCQ010000000
VERSION     DAORCQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_11505_cov_6.671441"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PKI23_08310"
     CDS             <1..1172
                     /locus_tag="PKI23_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNN36980.1"
                     /translation="QGLLEFVEMFKAPIKVLHPLLTATQEGNYNGTEGMGAIPFDGVI
                     LAHSNESEWQAFRNNRNNEAFIDRIYIVKVPYCLRLSDEVRIYEKLLTHSSLSKAPCA
                     PDTLKLLAQFSILTRLKEPENSNLYSKMRIYDGENLKNIDPKAKSYQEYRDAAGVDEG
                     MEGLSTRFAFKILSKVFNYDHTEVAANPVHLLHVLEEQIEQQQFGPERQDQYVRFIKE
                     YLVPRYIEFIGKEIQTAYLESYSEYGQNIFDRYVIYADYWIQDQEYRDHDTGEIFDRA
                     ALNEELEKIEKPAEISNPKDFRNEVVNFVLRAKANNEGKNPSWQSYEKLRGVIEKKMF
                     SNTEDLLPVISFNPKASKEEQQKHADFVTRMTERGYTEKQVRLLAEWYLRVRKSQ"
     gene            1189..2466
                     /locus_tag="PKI23_08315"
     CDS             1189..2466
                     /locus_tag="PKI23_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNN36981.1"
                     /translation="MTYIVDRRLNGKNKSSVNRQRFLQRYRAHIQKAVSDAVTGRKLT
                     DLDTGEKITIPKKEISEPLFQHGTAGNRRIVHPGNKEFVTGDQIARPNGGGGGGGGSG
                     DAGQDGEGEDEFSFSISRDEFLEFLFEDLALPNLIKRHLAGVDSFKLRRAGVVSDGSP
                     TKLNIVRSMRSSLARRIAIGAPLRAQLQELLDARESLLRSPEPALFERRIGELDEEIA
                     QLQSRLQKIPFLDSYDLRYNFHAKQPQPSAKAVMFCLMDVSGSMNQNMKEIAKRFFLL
                     LYLFLQRNYERTEVVFIRHHTSAKEVDEQEFFYSRETGGTIVSSALTLMHEIIQARYP
                     AGEWNIYGAQASDGDNWADDSPVCQALLTEKLLPLCQYYTYVEIMPRRHQSLWEHYEQ
                     VRQSHPDHFAMQDINDLPDIYPVFRQLFQRRLA"
     gene            2463..4010
                     /locus_tag="PKI23_08320"
     CDS             2463..4010
                     /locus_tag="PKI23_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNN36982.1"
                     /translation="MTLRQPISEGSEWSFELIDRYNQELARVAAHYRLDTYPNQIEII
                     SSEQMMDAYASVGMPLGYNHWSFGKQFVSVEQNYKRGRMGLAYEIVINSNPCIAYLME
                     ENTMMMQALVIAHACYGHNSFFKNNYLFKTWTDAGSIIDYLLFAKNYVRHCEERHGID
                     EVERLLDACHALMNHGVDRYKRPAPISPQEEQRRQQEREAYLQSQVNALWRTIPRSTS
                     EQAQQKQRLFPKEPQENLLYFIEKNAPLLEPWQREIVRIVRKLAQYFYPQRQTQVMNE
                     GWATFWHYTLLNHLYDEGRLTDGFMLEFLQSHTSVVAQPDFDSPHYRGINPYALGFAM
                     FRDIRRICEAPTDEDRRWFPDFAGADWLETLHFAMENFKDESFILQFLSPRLIREFRL
                     FCVLDDDRRDHLAIDAIHDDSGYRQIREQLSNQYNLSMNEPNIQAVRVDLHGDRSLTL
                     RHVQHNRRPLGKSTESVLKHLHQLWGFDVHLESTQDDRVQQTFHYPPAKGLDPAALLN
                     QFSFFNI"
     gene            complement(4026..4586)
                     /locus_tag="PKI23_08325"
     CDS             complement(4026..4586)
                     /locus_tag="PKI23_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN36983.1"
                     /translation="MTTLTFTDAPLLLVEDDAVFRDLLSQRLEAAGFTVQAAEDLESA
                     EQLVARQPFDYAILDLKLQQQSSLPLIAPLRQRNPRMRILMLTGYASIATAVSAIKLG
                     ADQYLAKPASSSEIITALTAPEESPAIEAEANVELNRMSVRRLEWEHIQQVLAENDGN
                     ISVTARQLQMHRRTLQRKLGKRPVGL"
     gene            complement(4583..5830)
                     /locus_tag="PKI23_08330"
     CDS             complement(4583..5830)
                     /locus_tag="PKI23_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN36984.1"
                     /translation="MLIPAGGRAEMLARLLVLRIIYLSLTAVATVAVALWLELLLPYW
                     ILAALLLAGVLSVLFNFARLRLPFPVTEGELAAHLAIDLLLLSAILAISGGSTNPLIS
                     FYLVLLAIASAILPGRFSYPLMATAILVYSAYLLHDLDDSAHHLHNPDAFRLHLVGMW
                     VIFVMSALLITLFVSRLAQAVRERDLALNQAREAVLRNEQLVAIGTLAAGTAHALGTP
                     LSTMLLLLEALKEESLEPPAERLVAQLSEQTERCRDELEQLKQEASAAAEPAPQWQPF
                     DQLAELLRQRLLLRAPDRLRQFTLEGSQPAPRLQVDPSLLHALLNLLENALQASKQGI
                     EVCCRWRAGEGQILIDDDGPGIDPAVMERLGEPFITARPGGLGLGLFIANATLERRGG
                     RVTLTQRAKAAGTHTCITLPLQP"
     gene            5938..6546
                     /locus_tag="PKI23_08335"
     CDS             5938..6546
                     /locus_tag="PKI23_08335"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNN36985.1"
                     /translation="MDRPFALQPVTLEGSLVTLRPLAVEDADPLAALVAAQRLDLHPH
                     TAIPAPQRMAHYIAEALDEQAAGRALPFVVIERATAQVAGTTRFCRAVPPHRRLEIGY
                     SWLGQQWRGRGINRQMKWLMLDHAFTALGALRVEFHTDLLNRQSQRALEKIGALREGL
                     LRHHMVMPSGRIRDSLIYSIVASDWPALAAGWAASDDPAPLD"
     gene            complement(6543..7310)
                     /locus_tag="PKI23_08340"
     CDS             complement(6543..7310)
                     /locus_tag="PKI23_08340"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNN36986.1"
                     /translation="MRLHNQRIIVTGGGSGFGKAMSQRFAEEGARLLLADFSAERGMA
                     VVEEIRRAGGEAHFCQIDVAQADQVKAMVAQAVTLWGGLDVMVNNAGYTHATMPLDAV
                     SEADYDRVFAVNVKSIYLSSREVVPLFRAQGEGVILNTASTAGLRPRPNLAWYNASKG
                     AVITLTKSMAIELAPAKVRVNCLCPVVANTPMLGDFIGDHARNTPETRAAMGAGIPLG
                     RLTEPQDVANAALYLVSDEAQFITGVALEVDGGRCIS"
     gene            complement(7341..8252)
                     /locus_tag="PKI23_08345"
     CDS             complement(7341..8252)
                     /locus_tag="PKI23_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNN36987.1"
                     /translation="MHQRPQRPRRSQLAVPGSDDKKMAKAAQSRADHVFLDLEDAVAP
                     SAKKPARQQIVTALTTLDWGHKVRCVRINDLETRYAYGDIIEVVSGARDKLDVLMIPK
                     VKRAADVIWVDILLSQLEADLGLDRPIGIEVLIEEVEAMMRVEEIAFSSPRLEALIFG
                     MGDYSASQGIDVRAIGGATSYPGDLWHYQRNKMVIAARAAGIDPIDGPFGNFRNEEGY
                     REECRRALTLGCVGKWAIHPAQIEPALEIFTPDPADVARARKMDEAYAEAEAAGIGAI
                     QVDGVLVDAASVRIQRNIVRRANLVGV"
     gene            complement(8269..9501)
                     /locus_tag="PKI23_08350"
     CDS             complement(8269..9501)
                     /locus_tag="PKI23_08350"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNN36988.1"
                     /translation="MKPLSGPGALAGLRVLDLTMMLSGPYCTMLLADQGADVIKIEPP
                     EGDGTRYSGPFADGDAARLFGGYFHSINRGKRSLVIDLKQAAGKALLQRLVRDADVLV
                     ENFRSGVMERLGLGYELLAGINPRLVYAAIRGFGDGRNGSSPYEAWPAYDVVAQAMGG
                     IMGITGPDAQSPMKIGPGVGDIFPGALNAFAILAAVIHARASGEGQFVDVSMTDAILS
                     LCERIVYQYSYQGTVPHPEGNAHPMLCPFGLFRCRDGWVTIACPRENFWSILCDEMNR
                     PELVSHERYRSNMQRALHRDEVNAEVERWTLRFSKQELVDRLGGKVPFAPVYDVADIF
                     ADPHFAARMLVPVEQPGAGRDVVLAGSPIGMSRTPPGIRGRGPLLGEHAEQVLGEWGL
                     TPAEIAQLRAEGVIQPSR"
     gene            complement(9498..9977)
                     /locus_tag="PKI23_08355"
     CDS             complement(9498..9977)
                     /locus_tag="PKI23_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNN36989.1"
                     /translation="MKLSAMTARDNYFEDFTVGDVMKHARSKTISVMENVLLTNLVMN
                     TAQGHFNEESMKGSAFGQRITFGGCTASLIIGLTSQDTSENALTELGLDRIRFKHPVL
                     HEDTLTAYTEVLEVKPAQREDAGEVRFRHWGVNQRGQVVFEGERRTLVKRRSHWGAR"
     gene            complement(9974..11137)
                     /locus_tag="PKI23_08360"
     CDS             complement(9974..11137)
                     /locus_tag="PKI23_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN36990.1"
                     /translation="MSHLTEERLMIQEAARQFSMKEVLPVANELDPIQGDIPMALRQK
                     LADMGYFGITIDPQYGGMGLGCFEYCLITEELSRGWMSVASLIARANGLGGASQLSEA
                     QRRDILPRTVRGEYLGAFALSEPGAGSDVAGLSCRAVRDGNDWVISGNKYWCTFADGA
                     DYIQVLCRTAPLDPKRRHAGISSIRIEKPRGELPAGCKGAPIPKIGYFGWKTWELAFD
                     GCRVPLDNLMGEEGAAFYAISQGLTVARAHTAARAIGLARGALEDALKYAQQREQFGR
                     PIGEFQAIRFKLATMATEIEAARQLMYHVCDLIDRGGRCDKEAGMAKYFATEVAERVT
                     SEAMQIFGGAGYTKLHAVERYWRDARLTKIFEGTSEIQQRIIADHLLGKGKSA"
     gene            complement(11134..>11505)
                     /locus_tag="PKI23_08365"
     CDS             complement(11134..>11505)
                     /locus_tag="PKI23_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNN36991.1"
                     /translation="SLFTTLTMNYNPLYFNQPYAEGHGHPAIVVNHMLVFTTVFGLSV
                     EDLSEAGGVFLGVEALSFHREVYPGDTLTARSEVLDKRESSSHPDSGIVTWHTSGYNQ
                     RGELVIDFRRTNMVKKREAKG"
BASE COUNT         2116 a         3847 c         3616 g         1926 t
ORIGIN      
        1 accaggggct gctcgaattc gtcgagatgt tcaaggcgcc gatcaaggtg ctgcatccgc
       61 tgctgaccgc cactcaggag ggcaactaca acggcaccga ggggatgggg gcgattccgt
      121 tcgacggcgt gattctggcc cactccaacg agtcggagtg gcaggcgttc cgcaacaatc
      181 gcaacaacga agccttcatc gaccgcatct acatcgtcaa ggtgccctac tgcctgcggc
      241 tctccgacga ggtgagaatc tacgaaaagc tgctgaccca cagctcgctc agcaaggcgc
      301 cctgcgcgcc cgacacgctc aagctgctgg cgcagttcag catcctgacc cggctgaagg
      361 agccagagaa ctccaacctc tactcgaaga tgcggatcta cgatggcgag aatctgaaga
      421 acatcgatcc gaaggcgaaa tcctatcagg agtaccgcga cgcggccggt gtcgacgagg
      481 ggatggaagg actgtcgacc cgcttcgcct tcaagattct ctccaaggtc ttcaactacg
      541 accacaccga ggtggcggcc aacccggtcc acctgctcca tgtgctcgag gagcagatcg
      601 agcagcagca gttcggtccg gagcggcagg atcagtatgt ccgcttcatc aaggagtacc
      661 tggtgccgcg ttacatcgag ttcatcggca aggagatcca gaccgcctat ctggagtcct
      721 actcggagta tgggcagaac atcttcgacc gctatgtcat ctacgccgac tactggatcc
      781 aggatcagga gtaccgcgac catgacaccg gcgagatttt cgaccgcgcc gcgctgaacg
      841 aggagctgga gaagatcgaa aaaccagccg agatcagcaa cccgaaggat ttccgcaacg
      901 aggtggtcaa cttcgtgctg cgtgccaagg ccaacaacga aggcaagaat ccatcgtggc
      961 agagctacga gaaactgcgc ggcgtgatcg agaagaagat gttcagcaac accgaggatc
     1021 tgctgccggt catctcgttc aatcccaagg cgtcgaagga ggagcagcag aaacatgccg
     1081 acttcgtcac ccgcatgacc gagcgcggct acaccgaaaa gcaggtcaga ctgctggccg
     1141 agtggtatct gcgggttcgc aagtcgcagt gaaccgacgg aggttgagat gacctacatc
     1201 gtcgatcgcc gcctcaacgg caagaacaag agcagcgtga accggcagcg gtttctgcag
     1261 cgctaccggg cgcacatcca gaaggcggtc tccgacgcgg tgaccgggcg caagctgacc
     1321 gacctcgaca cgggcgagaa gatcaccatc ccgaagaagg agatcagcga accgctgttc
     1381 cagcatggca ctgccggcaa ccggcgcatc gtccaccccg gcaacaagga gttcgtcacc
     1441 ggcgaccaga tcgcccgacc caacggcggc gggggcggtg gtggaggcag cggtgatgcc
     1501 ggccaggatg gcgagggtga ggatgagttc tcattctcga tctcgcgcga tgagtttctc
     1561 gaattcctgt tcgaggatct ggcgctgccc aatctgatca agcgccatct ggccggggtc
     1621 gacagcttca agctgcgccg ggccggcgtg gtcagcgatg gcagtccgac caagctcaac
     1681 atcgtgcgct cgatgcgctc gtcgctggcg cggcgcatcg ccatcggcgc accactgcgg
     1741 gcacagttgc aggagctgct cgacgcgcgt gagtcgctgc tgcgctcgcc ggagcccgcc
     1801 ctgttcgagc ggcgcatcgg tgaactggac gaggagatcg cccagttgca gtcgcggttg
     1861 cagaagattc cgtttctcga cagctacgac ctgcgctaca acttccacgc caaacagcct
     1921 caacccagcg ccaaggcggt gatgttctgc ctgatggatg tctccggttc gatgaaccag
     1981 aacatgaagg agatcgccaa gcgcttcttt ctgctgctct atctgttctt gcagcgcaac
     2041 tacgagcgca ccgaggtggt cttcatccgc caccacacca gcgccaagga ggtcgacgag
     2101 caggagttct tctactcgcg cgagaccggc ggcaccatcg tgtcgagcgc gctgacattg
     2161 atgcacgaga tcattcaggc acgctatccg gccggcgaat ggaacatcta cggcgcccag
     2221 gcctccgatg gcgacaactg ggccgatgac tcgccggtct gtcaggcgct gctcaccgaa
     2281 aaactgctgc cactctgcca gtactacacc tacgtcgaga tcatgccgcg ccgccaccag
     2341 tcgctgtggg agcactacga gcaggtgcgg cagagccacc ccgaccactt cgccatgcag
     2401 gacatcaacg acctccccga catctatccg gtgttccggc aactctttca aaggaggctg
     2461 gcatgacgct gcggcagccg atttcagaag gttcggagtg gagcttcgaa ctgatcgacc
     2521 gctacaacca ggagctggcg cgggtggcgg cacactaccg gctcgacacc tatccgaacc
     2581 agatcgagat catctcctcg gaacagatga tggatgccta cgcctcggtc ggcatgccgc
     2641 tcggctacaa ccactggtcg ttcggcaagc agttcgtctc ggtcgagcag aactacaagc
     2701 gcggccgcat ggggctggcc tacgagatcg tcatcaactc caacccctgc atcgcctacc
     2761 tgatggagga gaacacgatg atgatgcagg cgctggtgat cgcccatgcc tgctatggcc
     2821 acaactcctt cttcaagaac aactatctgt tcaagacctg gaccgatgcc ggctcgatca
     2881 tcgactacct gctgtttgcc aagaactacg tgcgtcactg cgaggagcgc cacggcatcg
     2941 acgaggtcga gcggctgctc gacgcctgcc acgccctgat gaaccacggc gtcgaccgct
     3001 acaagcgccc ggcaccgatc tcgccgcagg aggagcagcg gcggcagcag gagcgcgaag
     3061 cctacctgca gtcacaggtc aacgcgctgt ggcgcaccat tccgcgcagc accagcgagc
     3121 aggcgcaaca gaagcagcgg ctgttcccca aggagccgca ggagaatctg ctctacttca
     3181 tcgaaaagaa cgccccgctg ctggagccgt ggcagcgcga gatcgtgcgc atcgtgcgca
     3241 agctggcgca gtacttctat ccgcagcggc agacccaggt gatgaacgag ggctgggcca
     3301 ccttctggca ctacacgctg ctcaaccacc tctatgacga aggccggctc accgacggct
     3361 tcatgctgga gttcctgcag tcgcacacca gcgtcgtcgc ccagcccgac ttcgacagcc
     3421 cccactaccg tggcatcaac ccctacgccc tcggctttgc gatgttccgc gacattcgcc
     3481 gcatctgcga ggcccccacc gacgaagacc gccgctggtt ccccgacttc gccggcgccg
     3541 actggctgga gacgctgcac ttcgcgatgg agaacttcaa ggacgagagc ttcatcctgc
     3601 aattcctgtc gccccggctg atccgtgagt tccggctgtt ctgcgtgctc gacgatgacc
     3661 ggcgcgacca cctcgccatc gacgcgatcc atgacgacag cggctaccgg cagattcgcg
     3721 agcagctctc caaccagtac aacctgagca tgaacgagcc caacatccag gcggtgcggg
     3781 tcgacctgca tggcgaccgc tcgctgacgc tgcgccatgt gcagcacaac cgccggccgc
     3841 tgggcaagtc gaccgagagc gtgctgaagc acctgcacca gctgtggggc ttcgatgtcc
     3901 acctcgaatc gacccaggac gacagggtgc agcagacctt ccactatccg ccggccaagg
     3961 ggctcgaccc ggcggcactg ttgaatcagt tcagcttttt caacatctga atccgaagag
     4021 ggtggctaaa gtccgaccgg ccgcttgccg agcttgcgtt gcagcgtgcg gcggtgcatc
     4081 tgcaactgcc gggcggtgac cgagatgttg ccgtcattct cggccagcac ctgctggatg
     4141 tgctcccact ccagccggcg caccgacatc cggttgagct cgacgttcgc ttcggcctcg
     4201 atggccggac tctcctcggg tgcagtcagt gccgtgatga tctcgctgct gctggccggc
     4261 ttggcgaggt actgatcggc ccccagcttg atggcgctca ccgcggtggc gatgctggcg
     4321 tagccggtca gcatcagaat gcgcatccgc gggttgcgct ggcgcagcgg cgcaatcagc
     4381 ggcaggctgc tctgctgctg caacttcagg tcgagaatcg catagtcgaa tggctgccgg
     4441 gccaccagtt gctcggccga ctccagatcc tcggcggcct ggaccgtgaa accggctgct
     4501 tcgagccgct ggctcagcag atcacggaac accgcatcat cctcgaccag cagcagcggc
     4561 gcgtcggtaa aggtcaaagt ggtcatggct gcaacggcag cgtgatgcag gtgtgggtgc
     4621 cggccgcctt ggcgcgctgc gtcagggtca ccctgccgcc acgccgttcg agggtggcgt
     4681 tggcgatgaa gagccccagg ccaagaccgc cgggacgggc ggtgatgaag ggttcaccca
     4741 gccgctccat caccgccgga tcgatgcccg gaccatcgtc atcgatcaga atctgccctt
     4801 cgccggcgcg ccagcggcag cagacctcaa tgccctgctt cgaggcctgc aacgcattct
     4861 ccagcaggtt gagcagcgca tgcagcaggc tggggtcgac ctgcaaccgg ggcgccggtt
     4921 gactgccctc cagggtgaac tggcgcagcc ggtccggcgc acgcagcagc agccgctgtc
     4981 gcagcagctc ggccaactgg tcgaacggct gccactgcgg tgccggttcg gccgccgcgc
     5041 tggcctcctg cttcaactgc tccagctcgt cacggcagcg ctcggtctgc tcgctcaact
     5101 gcgccaccag ccgttccgcc ggcggctcca gcgactcctc cttcagcgct tccagcagca
     5161 gcagcatcgt cgacagtggc gtgccgagcg catgggcggt gccggcggcc agcgtgccga
     5221 tcgccaccag ttgctcgttg cgcagcaccg cctcgcgcgc ctgattgagc gccagatcgc
     5281 gctcgcgcac cgcctgcgcc agacggctga cgaacagcgt gatcagcaat gcactcatga
     5341 cgaagatcac ccacatgccg accagatgca ggcgaaaggc atcggggttg tgcaggtggt
     5401 gggcggagtc gtccagatcg tgcagcaggt aggccgaata gaccagaatc gccgttgcca
     5461 tcagcggata gctgaagcgc cccggcagaa tcgcgctggc gatcgccagc agcaccagat
     5521 agaaggagat cagcgggttg gtcgagccgc cggagatcgc cagaatcgcg ctcagcagca
     5581 gcaggtcgat cgccagatgg gccgccagct cgccctcggt cactggaaac ggcaaccgca
     5641 gtcgggcaaa gttgaacagc acgctcagca caccggccag cagcagggcg gcgagaatcc
     5701 agtagggcag cagcagctcc agccagagcg cgaccgcgac ggtggcaacc gcagtcagac
     5761 tcaggtagat gatgcgcaac accagcagcc gcgccagcat ctcggcgcgg ccacccgcgg
     5821 gaatcagcat gaaggatgac caggacatga cgcgaagtat aacgaccgca ttgacggtga
     5881 cgcagcggct gcgaccattt gtcgcggcct gccagccaca gccgacgagg tcatgcggtg
     5941 gataggccct ttgcgctgca accggtgacg ctcgaaggca gtctggtcac gctgcgcccg
     6001 ctggcggtgg aggacgccga cccgctggcg gcactggtgg cggcgcagcg gctcgacctg
     6061 catccgcaca cggcgattcc ggccccgcag cggatggccc actacattgc cgaggcgctg
     6121 gacgagcagg ccgccgggcg ggcgctaccc ttcgtcgtca tcgagcgcgc cactgcccag
     6181 gtggccggca ccacccgctt ctgccgcgca gtgccaccgc atcgccggct ggagatcggc
     6241 tacagctggc tcggccagca gtggcgcgga cgcggcatca accggcagat gaagtggctg
     6301 atgctcgacc acgccttcac cgctctgggt gcgctgcggg tggagttcca caccgacctg
     6361 ttgaaccggc agtcgcagcg ggcgctggag aagatcggcg cactgcgcga ggggctgttg
     6421 cgccaccaca tggtgatgcc gagcggccga atccgcgact cgctgatcta cagcatcgtc
     6481 gccagcgact ggccggcgct ggcagccggc tgggccgcca gcgacgatcc cgcgccgctc
     6541 gactaactga tgcagcggcc gccgtcgacc tccagcgcaa caccggtgat gaactgcgcc
     6601 tcgtccgaca ccagatagag cgcggcattg gccacatcct gcggctcggt cagacgcccg
     6661 agcggaatgc cggcccccat cgcggcacgg gtctccggcg tgttgcgcgc gtggtcaccg
     6721 atgaaatcgc ccagcatcgg cgtgttggcc accaccgggc agaggcagtt gacgcgcacc
     6781 ttggccggcg ccagttcgat cgccatcgac ttggtgaggg tgatcaccgc ccccttgctg
     6841 gcgttgtacc aggcgaggtt gggtcgcggc cgcagcccgg ccgtcgaggc ggtgttgaga
     6901 atcaccccct ctccctgggc gcggaacagc ggcaccacct cccggctgct caggtagatc
     6961 gacttgacgt tgacggcaaa gacccggtca tagtcggcct cgctgaccgc atcgagcggc
     7021 atggtggcat gggtgtagcc ggcgttgttg accatcacgt cgagcccgcc ccacagcgtg
     7081 acagcctgcg ccaccatcgc cttcacctga tcggcctgcg ccacgtcgat ctggcagaag
     7141 tgcgcctcgc cgccggcgcg gcgaatctcc tcgaccacgg ccatgccgcg ctcggcactg
     7201 aagtcggcca gcagcagccg ggcgccctcc tcggcaaagc gctggctcat cgccttgccg
     7261 aaaccggaac cgccaccggt gacgatgatt ctctggttgt gcaatcgcat gggggctctc
     7321 cttgggtacg gggttgagga tcagacgccg accagattgg cccgcctgac gatgttgcgc
     7381 tggatgcgca ccgaggcggc gtcgaccagc acgccatcga cctgaatcgc accgatgccg
     7441 gcggcctcgg cctcggcata ggcctcgtcc atcttgcggg cgcgggccac atcggccgga
     7501 tcgggggtga agatctccag cgccggctcg atctgcgccg gatgaatcgc ccacttgcca
     7561 acgcaaccga gcgtcagcgc gcgccggcac tcctcgcgat agccctcctc gttcctgaaa
     7621 ttgccgaacg ggccatcgat cggatcgatg ccggcggcgc gggcggcgat caccatcttg
     7681 ttgcgctggt agtgccagag atcgccgggg tagctggtgg cgccaccgat cgcccgcaca
     7741 tcgatgccct gcgaagcgga gtagtcgccc atgccgaaga tcagcgcctc cagccggggg
     7801 ctggagaagg cgatctcctc gacccgcatc atcgcctcga cctcctcgat cagcacctcg
     7861 atgccgatcg gtctgtcgag gccgaggtcg gcctccagct ggctcagcag gatgtcgacc
     7921 cagatcacgt cggccgcccg cttcaccttc gggatcatca gcacgtcgag cttgtcgcgg
     7981 gcgccgctga ccacctcgat gatgtcgcca taggcgtagc gggtctccag atcgttgatg
     8041 cgcacgcagc gcaccttgtg gccccagtcg agcgtggtca gcgccgtgac gatctgctgc
     8101 cgggccggct tcttggccga tggcgccacg gcatcctcca gatcgagaaa gacatggtcg
     8161 gcccggctct gcgccgcctt ggccatcttt ttgtcatcgc tgccgggcac cgccagttgg
     8221 gagcggcgcg gccgttgcgg gcgttgatgc atggtgcaga ctcctgattt atcgactcgg
     8281 ttgaatgacg ccttcggcac gcaactgggc aatttccgcc ggggtgagcc cccactcacc
     8341 cagcacctgc tcggcatgtt cccccagcag cggaccccgg ccgcgaatgc cgggcggcgt
     8401 tcggctcatg ccgatcggac tgccggccag caccacatcg cggccggcac cgggctgctc
     8461 gaccggcacc agcatccgtg cggcaaaatg gggatcggcg aagatgtccg ccacatcgta
     8521 gaccggcgcg aacggcacct tgccgccgag ccggtcgacc agctcctgct tgctgaagcg
     8581 gagcgtccag cgctccacct cggcattgac ctcgtcgcgg tgcagcgcgc gctgcatgtt
     8641 gctgcggtag cgctcatggc tgaccagctc cggccggttc atctcatcac agagaatcga
     8701 ccagaaattc tcgcgcgggc aggcgatggt gacccagccg tcacggcagc ggaacagccc
     8761 gaacgggcag agcatcgggt gggcattgcc ctcggggtgg ggcacggtgc cctggtagga
     8821 gtactggtag acgatccgct cgcagagcga caggatggca tcggtcatcg agacatcgac
     8881 gaactgcccc tcgccgctgg cgcgggcatg gatcaccgcc gccagaatcg cgaaggcgtt
     8941 gagcgcaccc ggaaagatgt cgccgacccc ggggccgatc ttcatcggcg actgcgcatc
     9001 gggtccggtg atgcccatga tgccgcccat cgcctgcgcc accacatcgt aggccggcca
     9061 ggcctcgtag gggctgctgc cgttgcgacc atcgccaaag ccgcggatgg cggcatagac
     9121 cagccgcgga ttgatgccgg ccagcagctc atagccgagg cccagccgct ccatcacccc
     9181 ggagcggaag ttctcgacca gcacatcggc atcacgcacc agccgctgca acagcgcctt
     9241 gcctgctgcc tgcttgaggt cgatcaccag gctgcgcttg ccgcggttga tgctgtggaa
     9301 gtagccaccg aacagccgcg ccgcatcgcc gtcggcaaag ggaccggagt agcgggtgcc
     9361 atcgccctcg ggcggctcga tcttgatgac gtcggcgccc tgatcggcca gcagcatcgt
     9421 gcagtagggg ccggagagca tcatcgtcag atcgagcacc ctgagccccg ccagcgctcc
     9481 cgggcccgac agcggtttca tcgtgcgccc cagtggctgc gccgcttcac cagcgtgcgc
     9541 cgctcgccct cgaacaccac ctgcccgcgc tggttgaccc cccaatgtct gaagcgcacc
     9601 tcgccggcat cctcgcgctg tgccggcttc acttcgagca cttcggtgta ggcggtcagg
     9661 gtgtcttcat gcagcaccgg atgcttgaag cggatcctgt cgagccccag ctcggtcagg
     9721 gcattctcgc tggtgtcctg gctggtgagt ccgatgatca gcgaggcggt gcagccgccg
     9781 aaggtgatgc gctgaccgaa ggcggagccc ttcatgctct cctcattgaa gtggccctgg
     9841 gcggtgttca tcaccaggtt ggtcagcagc acattctcca tcaccgagat ggtcttgctg
     9901 cgggcatgtt tcatcacgtc gccgacggtg aaatcctcga agtagttgtc gcgggcggtc
     9961 attgcggaga gtttcatgct gatttcccct tgccgagcag atggtcggcg atgatgcgtt
    10021 gctggatctc cgaggtgcct tcgaagatct tggtcaggcg ggcgtcacgc cagtagcgct
    10081 cgaccgcatg gagcttggtg tagccggcgc caccgaagat ctgcatcgct tccgaggtga
    10141 cccgctccgc cacctcggtg gcgaaatatt tggccatgcc ggcctccttg tcgcagcggc
    10201 caccgcggtc gatcaggtca cagacgtgat acatcaactg ccgtgccgcc tcgatctcgg
    10261 tggccatcgt cgccagcttg aagcggatcg cctggaattc gccgatcggc cggccgaact
    10321 gctcgcgctg ctgtgcatac ttcagcgcat cctcgagcgc gccgcgcgcc agaccgatcg
    10381 cccgggcggc ggtgtgggcg cgggccacgg tcagcccctg cgaaatcgca tagaaggccg
    10441 ccccctcctc acccatcaga ttgtcgagcg gtacccgaca gccatcgaag gccagctccc
    10501 aggtcttcca gccgaagtaa ccgatcttcg gaatcggtgc ccccttgcag ccggccggca
    10561 gttcgccgcg cggcttttcg atgcgaatcg aactgatgcc ggcatgacgt cgcttcgggt
    10621 cgaggggcgc ggtgcgacag agcacctgga tgtagtcggc gccatcggca aaggtgcacc
    10681 aatatttgtt gccgctgatc acccagtcgt tgccgtcgcg caccgcccgg cagctgagtc
    10741 cggccacatc ggagccggcg cccggttccg acagcgcgaa ggcgcccaga tactcaccgc
    10801 gcaccgtgcg cggcaggatg tcgcggcgct gtgcctcgct gagctggctg gcgccgccca
    10861 ggccgttggc ccgggcaatc aggctggcca cgctcatcca gccccgtgac agctcctcgg
    10921 tgatcaggca gtactcgaag cagcccagcc ccatgccgcc atattgcgga tcgatggtga
    10981 tgccgaaata gcccatgtcg gccagcttct gtcgcagcgc catcgggatg tcgccctgga
    11041 tcggatcgag ttcgttggcc accggcagca cctctttcat gctgaactgc cgcgcggcct
    11101 cctgaatcat caaccgctct tcggtgagat ggctcatccc ttggcctccc gctttttgac
    11161 catgttggtg cggcgaaagt cgatcaccag ctcgccacgc tggttgtaac ccgaggtgtg
    11221 ccaggtgacg atgccgctgt cggggtggga gctcgactcc cgcttgtcga gcacctcgct
    11281 gcgggcagtc agcgtgtcgc ccggatagac ttcgcggtga aaactgagcg cctcgacgcc
    11341 aaggaacacc ccgcccgcct cgctcagatc ctccaccgac agaccaaaga ccgtggtgaa
    11401 gaccagcatg tggttgacca cgatggccgg atggccatgc ccctcggcat agggctggtt
    11461 gaagtagagc gggttgtaat tcatcgtcaa ggtggtgaag agcga
//
LOCUS       DAORCQ010000073        11283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3820_length_11283_cov_6.613199, whole genome shotgun sequence.
ACCESSION   DAORCQ010000073 DAORCQ010000000
VERSION     DAORCQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3820_length_11283_cov_6.613199"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1011
                     /locus_tag="PKI23_08370"
     CDS             121..1011
                     /locus_tag="PKI23_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNN36992.1"
                     /translation="MQLHNLILSIQQIKTQLRKSERKVAEAVLARPAEIIHMRIVDLA
                     QEAQVSEPTVVRFCRSIKCNGFQDFKLRLAQQLATEASYDHAPVRPEDSIIQLRTKVF
                     DTTIGSLLAIRDRLDPTALERAIALLQQARRVEFYGFGASAAVATDAQDKFFRLKMMS
                     AAHCDPHIQCMSAQSLEPRDVVVAISQTGRTQALLHSVMLARQAGASVIALAPADSPL
                     AALSQVPIHLNVNEQNELHTPLTSRIVHLAVIDVLVMGVARQRGEGLLRHLAQLQQSL
                     EQLRVPPHGQQGKPLLESDE"
     gene            1008..1850
                     /gene="nfo"
                     /locus_tag="PKI23_08375"
     CDS             1008..1850
                     /gene="nfo"
                     /locus_tag="PKI23_08375"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNN36993.1"
                     /translation="MKYIGAHVSAAGGVEQAPLRAQQIGANAFALFTKNQRQWQAKPL
                     TEAEISAFRSGCERGGIAPAQILPHDSYLINLGHPDPAALDKSRAAFIDEMQRCRQLG
                     LTLLNFHPGSHLNQISEADCIARIAESINLALTAVPGVTAVIENTAGQGSNLGFRFEQ
                     LAAMIEQVDERGRVGVCFDTCHALAAGYDLRTAQGCQETFAEFDRTVGFRFLRAMHLN
                     GSKKGLGSRVDRHASLDEGELGMAVFHFIMADPRFDDIPLILETPNDARWAEEIALLR
                     SLAG"
     gene            1866..2486
                     /locus_tag="PKI23_08380"
     CDS             1866..2486
                     /locus_tag="PKI23_08380"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNN36994.1"
                     /translation="MANFDAIGNALADVGYAVLPDFISEPLQTALRQELLRRMAAGEL
                     KAAGIGRGADHRLLREVRGDQIRWLDGSTPAQARWLRRMRAVQRQLNRRLYLGLREHE
                     SQFAHFAPGTFYRRHLDSFAGRESRVVSTVLYLNRDWQPDDGGDLVLFDPATGAALRH
                     VQPLAGTLVIFMSALIPHEVEASRRARLSIAGWFRRDAVDDLPAPP"
     gene            complement(2507..3301)
                     /gene="thyA"
                     /locus_tag="PKI23_08385"
     CDS             complement(2507..3301)
                     /gene="thyA"
                     /locus_tag="PKI23_08385"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNN36995.1"
                     /translation="MRQYLELLRDILDHGVCKEDRTGTGTLSVFGRQLRFDLAQGFPL
                     LTTKRLHLRSIIHELLWFLRGDTNIAYLKAHGVSIWDEWADAQGDLGPVYGRQWRAWP
                     AANGERIDQLSQVLQTLKQNPDSRRMVVSAWNVAELPQMALPPCHLLFQFHVADGRLS
                     CQLYQRSCDTFLGVPFNIASYALLTQLVAAQCDLLPGEFIWTGGDVHLYLTPLDQARE
                     QLCREPYPLPRLLLKRRPASLFDYCYDDIAIVDYQSHPPIRAEVAV"
     gene            complement(3312..4118)
                     /gene="lgt"
                     /locus_tag="PKI23_08390"
     CDS             complement(3312..4118)
                     /gene="lgt"
                     /locus_tag="PKI23_08390"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNN36996.1"
                     /translation="MIAYPQIDPVAIALGPLKVHWYGLMYLLGFTAAWLLAKRRIQRG
                     GAPLQAEQVSDLIFYAALGVILGGRIGYVLFYNLPATLADPLSMFAVWQGGMSFHGGL
                     LGVLLAIWLFARRLNHHFLTTTDFIAPLVPPGLGAGRIGNFIGGELWGRATDLPWAML
                     FPRDPTQLPRHPSQLYQALSEGLLLFALLWWFSSGKRPRGAVSGLFLVGYGLQRFMIE
                     FVREPDVQIGFDLFGWLTRGQMLCLPMVIAGMLLLWMAYRLPATPSPSPH"
     gene            complement(4136..4927)
                     /locus_tag="PKI23_08395"
     CDS             complement(4136..4927)
                     /locus_tag="PKI23_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNN36997.1"
                     /translation="MITLLSYLAAGILAGLLAGLFGVGGGLIIVPTLAYIFKAQGVPA
                     TLLTHLAVGTSLSIITVTSISSVRSHHQHGAVRWPLFAQLAPGLVLGVFFGVITAGQL
                     SGATLQRLIGLFALAVAAQMLLKLQPRPHRQLPAAPLVAGTGGIIGYCSALFGIGGGS
                     LTVPFLTWNNVRMQEAVATSAACGLPIALFGALTNIWQGWHHADLPAYSSGFVYWPAF
                     AGIALTSTLAARWGARLAHRLSPERLKQAFGVMLALIGLQFILGI"
     gene            5041..5790
                     /locus_tag="PKI23_08400"
     CDS             5041..5790
                     /locus_tag="PKI23_08400"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNN36998.1"
                     /translation="MCLILLAHRYSDEFPLVVAANRDEFYARPTAGARFWDDTPTLLA
                     GRDLREGGTWLGITCSGRFAAVTNYRGSERRSRSRGQLTRDFLTSSIDPLAYLAQIHR
                     DRELYAGFNLLLGDGRGLYYYSNREGEIRSLQPGLYGLANHLLDTPWEKVELGKAALA
                     GRIAAGSRPGDLLEMLQQRPSLPVTAPPLTPEQLNAHRFIVSDSYGTCSSTVLTCSEA
                     GQIRWLEQRFKAGGIPDERHLYQFRLRRPGR"
     gene            6103..6687
                     /locus_tag="PKI23_08405"
     CDS             6103..6687
                     /locus_tag="PKI23_08405"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HNN36999.1"
                     /translation="MKLRNALLASAATLLGTMSIGAQAASENLLVDNSTYQTAQDVGA
                     VSSAAAVNVFGVRGTVSIFGVAINDDDNADFYQFSVGANQMVTLTVQTPEGSLENNDP
                     VVGLFDANGMQLANDDDGGAGYDSLLTYTLTTAGTYYAAVSGYADFDFIGGGSTNFIY
                     DLKIEAAPAPVPVPAAAWLFGSALLGLAASRRKS"
     gene            6850..8736
                     /locus_tag="PKI23_08410"
     CDS             6850..8736
                     /locus_tag="PKI23_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN37000.1"
                     /translation="MLTLEQISLHRGGRPVLTDCSLRINSGDRIGLVGANGAGKSSLM
                     QLILGQLAPDGGQIALQSGVRLAHLEQEHAAEQRCALEVVIDGDSQIGGLRQALAAAE
                     QTGDHEALATLHQQFAEVDGYRAESRAGEILHGLGVTPAQQRQSVRALSGGLRVRVQL
                     ARALMTPSDLLLLDEPTNHLDLDAILWLESWLARYPGSLLVISHDRDFFDHVVNQILH
                     LDSGVLTRYAGHYSDFERSRAEARRQQASAYQRQQQQLQQTQRFIDRFKAKASKAKQA
                     QSRMKALEKLERIVALREESTLRFSLPSAERISNPLLVLDRISVARGERTLIEHFSAT
                     LLPDTRVALLGRNGAGKSTLLAAIAGLLPLAGGTRLGGEHLKIGYFAQHQLEALDLTA
                     SPLLHLKRLDPRASEQSLRGFLGGFGFSAERALTAVEAFSGGEKSRLALALIAWQRPN
                     LLLLDEPTNHLDLDMRQALVEALLAFEGAVLLISHDRHLLESCAAEFWLIEAGRATLF
                     EGDLDDYARHLASSRSGDGSAPAADGASRRASRQNGAALRQQQAPLRQQVKKIEAEID
                     RLGRQLAQIEQQLAEPELYGGEHVARLRQLSQQQQHLRSALTEQEEQWMAASMQLETL
                     AADP"
     gene            8833..9240
                     /locus_tag="PKI23_08415"
     CDS             8833..9240
                     /locus_tag="PKI23_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNN37001.1"
                     /translation="MTQKPIEEGYFVIKEGGRPVLLGSFSPSTGNTYYPQRKLCPVTS
                     EPVQKVELPTEGELYSWTYVRMKMIGSTQSSDGGGHGVGQIDLPCGTRIQSIIRGEMG
                     DWKIGMKMRLALLPVKRDGDVELCSYCFEPVQA"
     gene            9251..10408
                     /locus_tag="PKI23_08420"
     CDS             9251..10408
                     /locus_tag="PKI23_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNN37002.1"
                     /translation="MSREVYVIGVGMHPFMRDAPPLREMYFTAASAALQDAGIRFQEV
                     GYLYNGYINGRMQGIGFAKDLGLTNVPVVHVENASATGSCAMREAVNAVKGGHCEVAM
                     AVGFDDMMKFSRARALSEMAEGPKIDSTILPAAFFAMWAVRRMHEHGTTLETFAKIAA
                     KNWNHARFNPMAQRQSKELVTPEEILAAPEVSYPHTTKMACAAGGGAAAAIVATKEVA
                     MRVARGRPMVKVVGSQMKSEHYTPGHVFLGAVIGPEDLTRVTTQALYEESGLGPKDVD
                     LVHVHDAFPIEELMYYELMGFAEPGEGDHLVARGDTSIGGKIPFSTDGGLTARGHPGG
                     PTGLAQIWDGVQQLRGESGPRQVAGARNAMIHMMGAGSICVMHMLQRDGDA"
     gene            10554..>11283
                     /locus_tag="PKI23_08425"
     CDS             10554..>11283
                     /locus_tag="PKI23_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37003.1"
                     /translation="MNIDFSAEELAFRAEVKAWFEANLPKELKAKEEAGESLTKEELI
                     SWGRKLNEKGWAAPSWPKEHGGTGWNATQKHIFEEVRAEVGAPQVFSMGITMLGPVLM
                     GYGNDAQRKQFLPRILNFDDWWCQGYSEPGAGSDLAALKTAAVLEGDHYVVNGSKIWT
                     TYAHRADWMFCLVRTNATGKKQQGISFLLIDMNTPGIQVRPIISIDGEHHLNQVFLDN
                     VRVPKQNLVGEENQGWTVAKYLLTH"
BASE COUNT         2150 a         3722 c         3582 g         1829 t
ORIGIN      
        1 agacgttcgt aataaacgag ttagcgaccg ggtgctgttc ccatcacccg ctccagctcc
       61 cgacccgcac cgtgactgtg cttcgtgctc aggaccggcc ttgccttcag gatgtttcgc
      121 gtgcagttgc acaacctgat cctgtcgatt cagcagatca agacccagct gcgcaagtcg
      181 gagcgcaagg tggccgaggc agtgctggca cgaccggcgg agatcatcca catgcgcatc
      241 gtcgatctgg cgcaggaggc gcaggtcagt gaaccgaccg tggtgcgctt ctgccgctcg
      301 atcaaatgca acggctttca ggatttcaag ctcagactgg cgcagcaact ggcgaccgaa
      361 gccagctatg accatgcacc ggtccggccg gaagactcga tcatccagct cagaacgaag
      421 gtgttcgaca ccaccatcgg ctcattgctg gcgattcgcg accgcctcga ccccacggcg
      481 ctggagcggg ccatcgcgct gctgcagcag gcacggcggg tggagttcta cggcttcggc
      541 gcctcggcgg cggtggcaac cgatgcgcag gacaagttct ttcggctgaa gatgatgagt
      601 gccgcccact gcgacccgca catccagtgc atgtcggcgc agtcgctgga accccgggat
      661 gtggtggtgg cgatctcgca gaccggacgc acccaggcgc tgctgcactc ggtgatgctg
      721 gcccggcagg ccggcgcgtc ggtgatcgcg ctggcaccgg ccgactcacc cttggcggcg
      781 ctgagccagg tgccgattca cctcaatgtc aacgaacaga atgagctcca caccccgctg
      841 acttcgcgga tcgtccatct ggcggtgatc gatgtgctgg tgatgggggt ggcccgccag
      901 cgtggcgagg ggctgttgcg tcacctcgcc cagttgcagc agagtctgga gcagttgcgg
      961 gtgccaccgc atggccagca aggcaaaccg ctcctggagt ccgacgaatg aagtacattg
     1021 gcgcccatgt cagcgccgcc ggcggcgtgg agcaggcgcc gctgcgtgcg cagcaaatcg
     1081 gcgccaacgc cttcgccctc ttcaccaaga accagcggca gtggcaggcc aagccgctga
     1141 ccgaggccga gatcagtgcg tttcgcagcg gctgcgagcg tggcggcatc gcacctgcgc
     1201 agatcctgcc gcacgacagc tatctgatca atctgggtca tcccgacccg gcggcgctcg
     1261 acaagtcgcg cgccgccttc atcgacgaga tgcagcgctg ccggcagctc ggcctgacgc
     1321 tgctcaattt ccatcccggc agccacctca accagatcag cgaggccgac tgcatcgccc
     1381 gcattgccga gtcgatcaat ctggcgctga cggccgtgcc cggcgtcacc gcagtgatcg
     1441 aaaacaccgc cgggcagggc agcaacctcg gttttcgctt cgagcagctc gcggcgatga
     1501 tcgagcaggt cgacgagcga ggccgggtcg gcgtctgctt cgacacctgc catgcactgg
     1561 cggccggcta cgacctgcgg acagcgcaag ggtgccaaga gaccttcgcc gaattcgacc
     1621 gcacggtcgg ttttcgcttt ctgcgcgcca tgcacctgaa tggctcgaag aagggtctcg
     1681 gcagtcgggt cgaccgccat gcctcgctcg acgaaggcga actgggaatg gcggtgttcc
     1741 acttcatcat ggccgatccg cgcttcgacg acattccgct gattctggaa acccccaacg
     1801 acgcgcgctg ggccgaggag atcgccctgc tgcgcagcct ggccggctga accggcagca
     1861 agcgcgtggc caacttcgat gcaatcggca atgccttggc cgatgtcggt tatgcggtgt
     1921 tgcccgattt catttccgag cccctacaga ccgccctgcg acaggagctg ctgaggcgga
     1981 tggcggcggg ggagctgaag gccgccggca tcggccgtgg tgccgaccat cggctgctgc
     2041 gcgaagtgcg cggcgatcag atccgctggc tcgatggcag cacgccggcc caggcccgct
     2101 ggctgcggcg gatgcgggca gtgcaacgac agctcaaccg gcggctctat ctgggtctgc
     2161 gggagcatga gagtcagttc gcccacttcg cgccaggcac cttctaccgt cgccacctcg
     2221 acagctttgc cggccgcgag agtcgggtgg tctccacggt gctctatctg aaccgcgact
     2281 ggcagcccga tgatggcggc gacctggtgc tgttcgatcc ggcaaccggc gcagcgctgc
     2341 gccatgtgca gccgctggcc gggacgctgg tgatcttcat gagtgcgctg attcctcacg
     2401 aggtggaggc cagccggcgc gcccgcctga gcatcgccgg ctggttccgg cgtgatgcgg
     2461 tcgatgacct gcccgcccca ccctgaaccg ggaccgccgt tgcgcgctac accgccacct
     2521 cggcgcggat cggcggatgt gactgatagt cgacaatggc gatgtcgtcg tagcagtagt
     2581 cgaacaggct ggccggccgg cgcttcagca gcagccgcgg cagcggatag ggctcgcggc
     2641 acagttgctc gcgcgcctga tcgagggggg tcaggtagag gtggacgtca ccgccggtcc
     2701 agatgaactc cccgggcagc aggtcgcact gcgccgccac cagctgcgtc agcaatgcat
     2761 agctggcgat gttgaacggc acccccagaa aggtgtcgca actgcgctga tagagctgac
     2821 aggagagccg cccgtcggcg acatggaact ggaacagcag gtggcagggc ggcagcgcca
     2881 tctgcggcag ttcggcgaca ttccaggcac tgaccaccat ccgccgcgag tcggggttct
     2941 gcttcagcgt ctgcaacacc tgactcaact gatcgatgcg ctcaccattc gcagcgggcc
     3001 aggcgcgcca ctgccggccg tagaccggac cgaggtcgcc ctgcgcgtcg gcccactcat
     3061 cccagatcga gacaccatgc gccttcaggt aggcgatgtt ggtgtcgccg cgcagaaacc
     3121 agagcagttc gtggatgatc gagcgcagat gcagccgctt ggtggtcagc aacggaaagc
     3181 cctgggccag gtcgaagcgg agctggcggc cgaacaccga caaggttccg gtgccggtgc
     3241 ggtcctcctt gcacacgccg tggtcgagaa tgtcccgcaa cagttccaga tattgacgca
     3301 tggccgtcga ctcagtgcgg tgaaggggag ggtgtcgccg gcaggcgata ggccatccac
     3361 agcaacagca tgccggcgat gaccatcggc agacagagca tctggccgcg cgtcagccag
     3421 ccgaacagat cgaaaccgat ctggacatcc ggctcacgca cgaactcgat catgaagcgc
     3481 tgcaggccat atcccaccag gaacagtccc gacaccgccc cgcgcggccg ttttccggac
     3541 gaaaaccacc agagcagcgc gaacagcagc agaccctcgc tgagtgcctg atagagctgt
     3601 gacggatgac gcggcaactg ggtcgggtcg cgcggaaaca gcatcgccca cggcagatcg
     3661 gtggcccgcc cccacagttc gccgccgatg aagttgccga tgcggccggc gccgagccct
     3721 ggcggcacca gtggcgcaat gaaatcggtg gtggtcagaa aatggtggtt cagcctgcgg
     3781 gcgaacagcc agatggccag cagcaccccc agcaggccgc catggaacga catgccaccc
     3841 tgccagaccg cgaacatcga cagcggatcg gccagcgtgg ccggcaggtt gtaaaacagc
     3901 acatagccga ttcggccgcc cagaatcacc cccagcgccg catagaagat caggtcactc
     3961 acctgttcgg cctgcagcgg cgcgccgccg cgctggatgc gccgcttggc cagcagccag
     4021 gcggccgtga agccgagcag gtacatcagt ccataccagt gcaccttgag gggccccagc
     4081 gcgatggcaa ccgggtcgat ctgtggatag gcgatcatgc tgcgtcctgt tctgctcaga
     4141 taccgagaat gaattgcagc ccgatcagcg ccagcatgac gccaaacgcc tgcttgaggc
     4201 gctccggcga cagccgatgg gccagccggg caccccagcg ggcggccagc gtgctggtca
     4261 gggcgatgcc ggcgaacgcc ggccagtaga cgaagccgct gctgtaggcg ggcaggtcgg
     4321 catggtgcca gccctgccag atgttggtca gggcgccgaa cagcgcgatc ggcaagccgc
     4381 aggcggccga ggtggccacc gcctcctgca tccgcacatt gttccaggtc agaaagggca
     4441 ccgtcagtga accgccgcca atgccgaaca gcgccgagca gtagccgatg atgccgccag
     4501 tcccggccac cagcggtgcc gcgggcagtt gtcgatgcgg tcgcggttgc agcttcagca
     4561 acatctgcgc ggccaccgcc agcgcgaaca gaccgatcag ccgttgcaat gtggcgccgg
     4621 agagctgacc cgcggtgatg acgccgaaaa agacgcccag caccagtccc ggcgccagtt
     4681 gcgcgaagag cggccagcgc accgccccat gctgatggtg gctgcggacc gagctgatcg
     4741 aggtgacagt gatgatcgac agcgaggtgc cgaccgccag atgggtcagc agcgtggccg
     4801 gcaccccctg cgccttgaag atgtaggcca gcgtcggcac gatgatcaga ccgccgccaa
     4861 cgccgaacag tccggccagc aggcccgcca gaatgccggc agcgaggtaa ctcagcagcg
     4921 tgatcatgca gcgacctttg atgtgggtcg gcattatggc cgacccgact gctttgataa
     4981 actcggacag ccgttggccc acgcgccatc aaccggaagc agtgactgga gcgacctgcg
     5041 atgtgcctga ttctgctcgc ccaccgttac agcgatgaat tcccgctggt ggtggcggcc
     5101 aaccgcgacg agttctacgc acgcccgacc gctggcgccc ggttctggga cgacaccccc
     5161 accctgctgg ctggacgcga cctgcgcgaa ggcggcacct ggctcggcat cacctgttct
     5221 ggccgcttcg cggcagtgac caactaccgc ggcagcgagc gtcgctcccg ctccagaggg
     5281 cagctgaccc gtgactttct gaccagttcg atcgacccgc tggcctacct ggcgcagatc
     5341 caccgcgacc gcgaactcta cgccggcttc aacctgctgc tgggcgatgg ccgcggactc
     5401 tactactact ccaaccgcga aggcgagata cgctcgctgc aaccgggact ctacgggctg
     5461 gccaaccacc tgctcgacac cccgtgggag aaggtcgaac tcggcaaggc cgcgctggcc
     5521 ggacggatcg ccgccggctc ccgaccgggc gacctgctgg agatgctgca acagcggcca
     5581 tccctgccag tcactgcacc gccactgacc cccgagcagc tgaatgccca ccgcttcatc
     5641 gtcagcgaca gctacggcac ctgctccagc accgtgctca cctgctccga agcgggccag
     5701 attcgctggc tggagcagcg cttcaaggcc ggcggcatcc ccgatgagcg ccacctctac
     5761 cagtttcggc tgaggcggcc gggccgatga caatgttcag ctgatccgtt ttgcaacagg
     5821 catctctgcc acttcactgt tgcatccagt cccgtgatgc aggcggatcg gaacaacctg
     5881 gagaatgcga agcaccgacc gaaaagtgtc agcttttttt acactattca aatctgacac
     5941 tgaaacgctt gctccactta tgaatcagcc agatgattta taataaattt ttaatgttgg
     6001 ctcaggcttt gctgtaaact gaaaaccggc ggcattgcct aatcgtcggc cacagccgac
     6061 cggcgtgatt cggaacagga caacctctac aggaaatgaa aaatgaagct acgtaatgca
     6121 ctgctggcat cggccgcgac tctgctcggc accatgtcga tcggcgccca ggccgccagc
     6181 gaaaacctgc ttgtcgacaa cagcacctat caaaccgcac aggatgtcgg cgccgtgagt
     6241 tcagcggctg ccgtcaacgt gttcggtgtg cgtggcaccg tctcgatctt tggcgttgcc
     6301 atcaacgatg acgacaatgc cgatttctac cagttctcgg tcggcgcgaa ccagatggtc
     6361 accctcacgg tgcagacccc cgaaggttca ctggagaaca acgatccggt cgttggcctg
     6421 ttcgacgcca atggcatgca actggccaat gacgatgacg gtggcgcggg ctatgacagc
     6481 ctgttgacct acaccctcac caccgccggc acctactatg ccgcagtgtc cggctatgcc
     6541 gacttcgact tcattggcgg cggcagcacc aacttcatct atgatctcaa gatcgaggct
     6601 gcacccgccc cggtaccggt gccggccgct gcctggctgt tcggttcggc gctgctgggc
     6661 ctggcagcca gccgccgcaa gtcataagag tgcgtcaggc ggccatgtag ccgctgccaa
     6721 agcacgattg cgacagcgag gagccccgcc atcactggcg gggctccttt ttgcacttcc
     6781 agaaatgggt tcgaccgcaa cgcacccatt accgacaccc gcttcaccca tcccgcatcg
     6841 cctcatctca tgctgaccct cgagcagatc tccctccatc gcggcggccg gccggtattg
     6901 accgactgct cgctgcggat caacagcggt gaccgcatcg gtctggtggg agccaacggt
     6961 gccggcaaga gcagcctgat gcagctgatt ctgggtcagc ttgcacccga cggtggccag
     7021 attgcgttgc agagcggtgt gcggctggcc cacctcgaac aggagcacgc ggccgagcag
     7081 cgctgcgcgc tggaggtggt gatcgatggc gattcgcaga tcggcggcct gcgacaggcg
     7141 ctggccgccg ccgagcagac gggcgaccac gaggcactgg ccacgctgca tcagcagttc
     7201 gccgaggtcg atggctaccg ggccgagagc cgcgccggcg agattctgca tgggctgggc
     7261 gtcaccccgg cccagcagcg gcagtcggtg cgcgcgctct ccggcggctt gcgggtgcgg
     7321 gtgcagttgg cgcgggcgct gatgacccct tcggatctgc tgctgctcga tgaaccgacc
     7381 aaccacctcg atctcgatgc gattctctgg ctcgaaagct ggctggcgcg ctatcccggc
     7441 agcctgctgg tgatctccca cgaccgcgac ttcttcgacc atgtggtcaa ccagattctg
     7501 cacctcgaca gcggcgtgct gacccgttac gccggccact acagcgactt cgagcgcagc
     7561 cgtgccgagg cgcggcgaca gcaggccagc gcctaccagc gccagcagca gcaacttcag
     7621 cagacccagc gcttcatcga ccgcttcaag gccaaggcca gcaaggccaa acaggcacag
     7681 agccggatga aggcactgga gaagctcgaa cggatcgtcg cgctgcgcga ggagagcacg
     7741 ctgcgcttca gcctgcccag tgccgagcgc atctccaatc cgctgctggt gctcgatcgg
     7801 atcagcgtcg cccgcggtga acggaccctc atcgagcact tttcggccac gctgctgccc
     7861 gacacccgcg tcgccctgct cggtcgcaat ggcgccggca agtcgacact gctggccgcc
     7921 atcgccggcc tgctgccgct ggcaggtggc acgcggctgg gcggagagca tctgaagatc
     7981 ggctacttcg cccagcatca gctcgaagcg ctcgacctga ccgcttcacc cctgctgcat
     8041 ctgaagcggc tcgatccccg cgccagtgag cagtcactgc gcggctttct gggcggtttc
     8101 ggcttcagtg ccgaacgcgc gctgactgcg gtcgaagcct tctccggtgg tgagaagagc
     8161 cggctggcac tggcgctgat cgcctggcaa cggcccaacc tgctgctgct cgacgagccg
     8221 accaaccacc tcgacctcga catgcgtcag gcgctggtgg aggcgctgct ggccttcgag
     8281 ggcgccgtgc tgctgatctc ccacgaccgc catctgctcg agagctgtgc cgccgaattc
     8341 tggttgatcg aagcgggacg ggcgaccctt ttcgagggtg atctcgacga ctatgcccgc
     8401 caccttgcca gttcacgcag cggcgatggc agcgcgccag cggccgatgg cgcctccaga
     8461 cgggcctcgc ggcagaacgg ggccgccctg cgccagcagc aggcgccgct gcgacagcag
     8521 gtgaagaaaa tcgaagccga aatcgatcgc ctcggtcgac aactggccca gatcgagcag
     8581 caactcgccg agcccgagct ctacggtggc gaacatgtcg ccagactccg gcaactgtcg
     8641 cagcagcagc agcatctgcg cagcgcgctg accgagcagg aagagcagtg gatggcagcg
     8701 agcatgcaac tggaaacact cgccgccgac ccttgagcag cacaacggcc aaggtggttt
     8761 cgcccgactg ccgcactggc tagaatagct gcgccccgat tcagcaatcc aacaataaga
     8821 tgaggatggc ccatgaccca aaagccaata gaggaaggct acttcgttat caaagagggg
     8881 ggcaggccag tgctgctcgg ctccttcagt ccctcgaccg gtaacaccta ctacccccag
     8941 cgcaagctct gcccggtcac ctccgagccg gtgcagaagg tcgaactgcc gaccgaaggc
     9001 gagctctact cctggaccta cgtgcgcatg aagatgatcg gttcgaccca gtcctccgac
     9061 ggcggcggcc atggcgtcgg ccagatcgac ctgccgtgtg gcacccgcat ccagtcgatc
     9121 attcgcggcg agatgggtga ctggaagatc ggcatgaaga tgcggctggc cctgctgccg
     9181 gtcaagcgcg atggcgacgt ggagctctgc tcctactgtt tcgaaccggt tcaggcatag
     9241 gagagccccc atgagcagag aagtctatgt gatcggcgtc ggcatgcacc ccttcatgcg
     9301 cgatgcgcca cctttgcgcg agatgtactt caccgccgcc agcgcggcac tccaggatgc
     9361 cggcatccgc ttccaggagg tgggttacct ctacaacggc tacatcaacg gccggatgca
     9421 gggcatcggc ttcgccaagg atctcggcct gaccaacgtg ccggtggtcc atgtcgagaa
     9481 tgcctcggcg accggctcct gtgcgatgcg cgaggcggtc aatgccgtca agggcggcca
     9541 ctgcgaggtc gccatggcgg tcggcttcga cgacatgatg aagttcagcc gggcacgggc
     9601 actgagcgag atggccgaag gtccgaagat cgattcgacc atcctgccgg cggctttctt
     9661 cgccatgtgg gcagtgcggc ggatgcacga gcatggcacc accctcgaga ccttcgccaa
     9721 gattgcggcc aagaactgga accatgcccg cttcaacccg atggcgcagc ggcagtcgaa
     9781 ggagctggtg acaccggaag agattctggc cgcacccgaa gtctcctatc cccacaccac
     9841 caagatggcc tgtgcggccg gcggtggcgc ggcagcggcc atcgtcgcca ccaaggaggt
     9901 ggcgatgcgg gtggcacgcg gccggccgat ggtgaaggtg gtcggttcgc agatgaagtc
     9961 cgaacactac acgccgggcc acgtctttct gggggcggtg atcggccccg aggatctgac
    10021 ccgggtcacc acccaggcgc tctacgaaga gagtggcctt gggccgaagg atgtcgatct
    10081 ggtccatgtg catgacgcct tcccgatcga agagctgatg tactacgaac tgatgggctt
    10141 tgccgagccc ggcgaaggcg accacctggt ggcccggggc gacacctcga tcggtggcaa
    10201 gattcccttc tccaccgatg gcggactcac cgcgcgcggc caccccggcg gcccgaccgg
    10261 actggcccag atctgggacg gcgtgcagca gttgcgcggc gaatcgggtc cgcggcaggt
    10321 ggccggcgcg cgcaacgcca tgatccacat gatgggggca ggctctatct gcgtgatgca
    10381 catgttgcag cgcgatggcg atgcctgagc cgacgtgaag cggtcatcgg ccgatgaccg
    10441 ccttttttca ggcaagatcg agcggggagc atgggctccc cgttccgttc atgtgataat
    10501 cggcacccgt cacaggcggc tctagcctgg acggaacaca acagaggtca accatgaata
    10561 tcgatttttc agccgaagag ctggcattca gagccgaggt caaggcctgg ttcgaggcca
    10621 acctgcccaa ggagttgaaa gcaaaggaag aagccggcga atcgctcacc aaggaagagc
    10681 tgatcagctg gggacgcaaa ctgaacgaga agggctgggc ggcaccctcc tggcccaagg
    10741 agcatggcgg caccggctgg aatgccaccc agaagcacat tttcgaagag gtccgtgccg
    10801 aagtcggcgc accacaggtg ttctcgatgg gcatcaccat gctgggaccg gtgctgatgg
    10861 gctatggcaa cgatgcacag cgcaagcagt tcctgccgcg cattctcaac ttcgatgact
    10921 ggtggtgcca aggctactcc gaaccgggcg ctggctccga cctggcggct ctcaagaccg
    10981 ccgccgtgct cgaaggtgac cactacgtgg tcaacggctc caagatctgg accacctacg
    11041 cccaccgcgc cgactggatg ttctgcctgg tgcgcaccaa cgcaaccggc aagaagcagc
    11101 agggcatcag ctttctgctg atcgacatga acaccccggg cattcaggtc aggccgatca
    11161 tctcgatcga tggcgaacac cacctcaacc aggtcttcct cgacaacgtg cgggtgccga
    11221 aacagaacct ggtcggcgag gagaaccagg gctggaccgt cgccaagtac ctgctcaccc
    11281 acg
//
LOCUS       DAORCQ010000074        11246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3844_length_11246_cov_5.692699, whole genome shotgun sequence.
ACCESSION   DAORCQ010000074 DAORCQ010000000
VERSION     DAORCQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_11246_cov_5.692699"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /gene="rnc"
                     /locus_tag="PKI23_08430"
     CDS             complement(<1..472)
                     /gene="rnc"
                     /locus_tag="PKI23_08430"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNN37004.1"
                     /translation="MNRPLSSLQQRLGYLFNDPRLLEEALTHSSHASGSNERLELLGD
                     SVLGFVITEELFNRFKSAREGKLTRLRARLVRGQTLSELAREFELGDYLRLGPGELKS
                     GGFRRDSILADAMEAVIGAIYLEAGFDECRRHLLNWFEPRLSQLSLDDPQKVLFA"
     gene            complement(469..843)
                     /locus_tag="PKI23_08435"
     CDS             complement(469..843)
                     /locus_tag="PKI23_08435"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNN37005.1"
                     /translation="MTTLTRQRGASMLAMLYFLTVTGFLLLLAFRLVPLYLDNSFVAA
                     AVKSLEEVPQIGEKSPSEVKNLLMTRFQINNIRNVDGKAVKVEEVDGKLVVDVNYDVA
                     VPIIANVEALVHFKNHFEARRP"
     gene            complement(872..1645)
                     /gene="lepB"
                     /locus_tag="PKI23_08440"
     CDS             complement(872..1645)
                     /gene="lepB"
                     /locus_tag="PKI23_08440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNN37006.1"
                     /translation="MNIDLPLILVVGTAVTGLVVLADRLWLAARRQAQATSVAAAPSE
                     PVWVEYSKSFFPVLLIVLVLRSFLFEPFQIPSGSMIPTLEVGDFILVNKYSYGVRLPV
                     VGTKVVAVGDPQRGDVMVFRPPHKPDTNFIKRVIGVGGDTVRYENRRLTINGQPVPQR
                     FVAALPVEAPQLELLQETLGSVSYAIQHTAPLQGRVREWVIPQGYYFVMGDNRDNSSD
                     SRVWGLVPDELVVGKAFVIWMHWPSFWSIPSFGRDGLIH"
     gene            complement(1645..3435)
                     /gene="lepA"
                     /locus_tag="PKI23_08445"
     CDS             complement(1645..3435)
                     /gene="lepA"
                     /locus_tag="PKI23_08445"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNN37007.1"
                     /translation="MQQIRNFSIIAHIDHGKSTLADRFIQICGGLSEREMSAQVLDSM
                     DIERERGITIKAQSVTLHYKARDGLTYQLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAAQGVEAQSVANCYTAIEQGLEVLPVLNKIDLPQADPERVCREIEEIIGIDTHQAVL
                     VSAKTGQGVEELLEKLIEVMPPPVGDAAAPLQALIIDSWFDNYLGVVSLVRVMQGQIK
                     KGDKILIKSVGRSHQAEGIGIFTPKRTETGLLSAGEVGYVVAGIKEVKGAPVGDTITH
                     AATPEVEALAGFARIKPQVYAGLFTVNSEDYEAFREALSKLTLNDASLFYEPESSDAL
                     GFGFRCGFLGMLHMEIIQERLEREYDLNLITTAPTVVYEVLTKEQKVVLVDNPSKLPD
                     PNQILEVREPICRANILVPQQYLGGVINLCVEKRGVQKDLQFHGNQASLVYELPMSEV
                     VLDFFDRLKSVSRGYASLDYGFERFEPASVVRLDILINGDKVDALAVIVHRQQAQQRG
                     RQVAEKLRELIPRQMFDVAIQAAIGGQVLARTTVKALRKNVTAKCYGGDVSRKRKLLE
                     KQKEGKKRMKQLGSVEVPQEAFLAVLKVDK"
     gene            complement(3650..4030)
                     /gene="gloA"
                     /locus_tag="PKI23_08450"
     CDS             complement(3650..4030)
                     /gene="gloA"
                     /locus_tag="PKI23_08450"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNN37008.1"
                     /translation="MRILHTMLRVTDLDRSVDFYQRVLGMRLLRRKEYPQGRFTLAFL
                     AFDDDPGVQIELTHNWDTDHYELGNGYGHIAIEVEDVHAACERIRAAGGVISREPGPM
                     QHGSTLLAFVKDPDGYSIELLGAG"
     gene            complement(4048..5262)
                     /locus_tag="PKI23_08455"
     CDS             complement(4048..5262)
                     /locus_tag="PKI23_08455"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNN37009.1"
                     /translation="MSAIKKVVLAYSGGLDTSVIAHWLRETYHCEVVTFTADLGQGEE
                     LEPARAKAQAMGIKEIYIEDLRETFARDYVFPMFRANTLYEGEYLLGTSIARPLIARR
                     LVEIAEETGADAISHGATGKGNDQVRFELGAYALKPGIRVIAPWREWTLNSREKLLAY
                     CEQHGIAVEQKRGNASPYSMDANLLHISYEGAVLEDPWAEPEEGMWRWSVSPELAPDH
                     AVEVELTFVNGDVVAIDGVAKSAAEVMLELNRLGGAHGVGRTDIVENRYVGMKSRGCY
                     ETPAGTILLKAHRAIESITLDREVAHLKDELMPRYASLIYNGYWWSPERLCLQALIDQ
                     SQQHVNGKVRLKLYKGNVSVVGRASDESLFDASIATFEDDQGAYDQADAGGFIKLNAL
                     RLRIAARRGRKL"
     gene            complement(5285..6061)
                     /locus_tag="PKI23_08460"
     CDS             complement(5285..6061)
                     /locus_tag="PKI23_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNN37010.1"
                     /translation="MSTLTLALVQADLAWHDIEANLTHFDQLLAPLAAVDLIVLPEMF
                     TTGFTMASQQQAEPMAGRGIAWLRTTAQARNAVVCGSLAIEEQGLCYNRFVWATPDGA
                     LHHYDKRHCFRMAGEHHHYRSGESAVVMSLNGFRIRPIICYDLRFPVWCRNRDDYDLL
                     LCVANWPAARSSAWQRLLPARAIENLSYVAAVNRVGLDGKGHAYSGDSALYDWLGEPL
                     LAAAPGEEALLTATLDLAALQRFRAGFPAHLDADPFEFTH"
     gene            complement(6049..7212)
                     /locus_tag="PKI23_08465"
     CDS             complement(6049..7212)
                     /locus_tag="PKI23_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNN37011.1"
                     /translation="MTLPMIRPLPSKLPQVGTTIFTRMSALAAECGAINLSQGFPDFD
                     GPALLRERLHHYVEQGYNQYAPMTGVATLREAIAEQVARRYRRRVDAEQEVTVTSGAT
                     EALYAAITAVVRPGDEVILFDPAYDSYEPAIELSGGTARHIALEAPEFRIDWNRVADC
                     ISDRTRLIIINSPHNPTGSVLAGEDLQQLAALVRERDIWLIADEVYEYIHFDGLDHAS
                     FHRHAELAARSFIISSFGKSFHITGWKLGYCIAPPPLSAEFRKVHQFLTFASFTPAQW
                     AIADTLRAQPEHLAGLPAFYQQKRDLFAAALKPSRFELLPCRGTYFQLAAYDAIVDLN
                     DVEFAQWLTREAKVAVIPVSVFYQTPPATRIVRFCFAKSDAVLLQAAERLCRL"
     gene            7355..7978
                     /locus_tag="PKI23_08470"
     CDS             7355..7978
                     /locus_tag="PKI23_08470"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF006672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HNN37012.1"
                     /translation="MAQPDDVDQLAERLVETVRFFAENGHCPATGGNFSLRSGDRVLI
                     TASGVDKRSLTRAQLLGLTLDGQLCAPGKPSAETELHLALYRADADIGAVLHIHSVAA
                     TVLSRLIDGDTLPLHGYEMQKAISGIDSHQQTLLLPLVDNSQQMACIAEQIERQWSTA
                     QRAHAVLVRGHGLYAWGHDLAAARRHLEGWEFLLQCELNRRLLERNP"
     gene            7975..8667
                     /gene="mtnC"
                     /locus_tag="PKI23_08475"
     CDS             7975..8667
                     /gene="mtnC"
                     /locus_tag="PKI23_08475"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003144789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HNN37013.1"
                     /translation="MTLRAIVTDIEGTTTDIHFVHRVLFPYAAERLEGFIRQHGDEAE
                     VAALLDQVREEIRQPQATAAALVDTLQQWIKLDLKLTPLKSLQGLIWREGYQQGHFTA
                     HVYPEVAAALRDWQRRGLALYVYSSGSVEAQRLLFAHTQDGDLTPLFSGYFDTRIGAK
                     RERSSYDRIAEQIGLAGDEILFLSDVEAELDAAAAAGFHTCLLLRDGANGSGHHPSAH
                     DFEQIPLLQEQP"
     gene            8664..9215
                     /locus_tag="PKI23_08480"
     CDS             8664..9215
                     /locus_tag="PKI23_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HNN37014.1"
                     /translation="MSHLTRYHQDRPEQPLGNTSDAEEIRALLAEAGVRFERWRADRP
                     LPADADQAQIGAAYRSEIDRLMREGGYQSWDVISMRPDHPDRLAMRQKFLDEHTHAED
                     EVRFFVAGEGLFNLHLGETVFALLCQREDLISVPAGTRHWFDMGDQPDFIAIRLFTNP
                     EGWVARMTGDGIGRRFPSIDHYR"
     gene            9296..10543
                     /locus_tag="PKI23_08485"
     CDS             9296..10543
                     /locus_tag="PKI23_08485"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNN37015.1"
                     /translation="MALYVQKFGGTSVGTLPRIEAVAEKVASYRNQGHDLVVVVSAMS
                     GETNRLLGLARDLADPADPRELDALVCTGEQVTAALLAMALIKRGCPARSMNGAQVPI
                     LTDNAFTKARIERIDDQALRAALAAGEVVVVTGFQGVDAEGNVTTLGRGGSDTTAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVERARRLERITFEEMLEMASLGSKVLQIRSVEF
                     AGKYAVPLRVLSSFVEGSGTLITLENPNMMENPIISGIAFNRDEAKVTVLGVPDVPGV
                     AARILCPISDANIEVDVIVQNVAADRSTDFTFTVHRNDLQKALAILNAQAGALNAREI
                     KGDRSVVKVSLVGVGMRSHAGIASKMFATLAEVGINIQIITTSEIKISVVIDEKYLEL
                     AVRSLHTAFGLDTPEEIAVVEEG"
     gene            10625..10813
                     /gene="csrA"
                     /locus_tag="PKI23_08490"
     CDS             10625..10813
                     /gene="csrA"
                     /locus_tag="PKI23_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNN37016.1"
                     /translation="MLILTRRVGETLIIGDDITVTVLGVKGNQVRLGVDAPKEVSVHR
                     EEIYQRIQAEKRQGHDDE"
     gene            10949..11039
                     /locus_tag="PKI23_08495"
     tRNA            10949..11039
                     /locus_tag="PKI23_08495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10983..10985,aa:Ser,seq:gct)
BASE COUNT         2127 a         3668 c         3463 g         1988 t
ORIGIN      
        1 gggcgaacag caccttctgc ggatcatcga gcgacagttg cgacagccgc ggttcgaacc
       61 agttgagcaa gtgacggcgg cactcatcga aacccgcctc cagatagatg gcgccaatga
      121 ctgcctccat cgcgtcggcc agaatcgaat cacggcgaaa cccgccgctc ttgagctctc
      181 cgggtcccag ccgcaggtag tcgcccagtt cgaactcccg cgccagctcc gagagggtct
      241 gcccgcgcac cagccgggcg cgcagccggg tcagcttgcc ttcacgcgcg ctcttgaagc
      301 ggttgaacag ctcctcagtg atgacgaagc cgagcaccga gtcacccagc agttcgagcc
      361 gctcgttgct gccactggca tggctgctgt gggtcaatgc ctcttcgagc agtcgcgggt
      421 cgttgaacag ataaccaagt cgctgctgga gactggagag cggtctgttc aagggcgccg
      481 ggcttcgaag tggttcttga aatggaccag cgcctcgacg ttggcgatga tcggcaccgc
      541 cacgtcgtag ttgacatcga ccaccagctt tccatcgacc tcctcgacct tcaccgcctt
      601 gccgtcgaca ttgcggatgt tgttgatctg aaagcgggtc atcagcagat ttttcacttc
      661 actgggcgat ttctcgccaa tctgcggcac ctcttcgagc gacttcaccg ccgccgcgac
      721 aaaggagttg tcgagataga gcggtaccag cctgaatgcc agcaacaaca gaaaaccggt
      781 gacggtcaga aaatagagca tcgccagcat cgatgcgccg cgctgccggg tgagtgtcgt
      841 catcgctgtt cctccgaagc tgaaacccaa ctcaatgaat cagaccatcg cggccaaaac
      901 tcggaatcga ccagaagctc ggccagtgca tccagatcac gaacgccttg cccaccacca
      961 gttcatcggg caccagaccc cagacgcggc tgtcgctgct gttgtcacgg ttgtcgccca
     1021 tcacgaagta gtagccttgc ggaatgaccc actcgcgcac ccgcccctga agcggcgccg
     1081 tgtgctggat ggcatagctg acgctgccga gtgtctcctg cagcagttcg agctgcggcg
     1141 cctcgaccgg cagggcggcg acgaaccgtt gcggcaccgg ctggccattg atcgtcagtc
     1201 gccggttctc gtagcgcacc gtatcgccac ccacgccgat gacccgcttg atgaagttgg
     1261 tgtcaggctt gtgcggcggc ctgaacacca tcacatcacc ccgctgcgga tcacccaccg
     1321 caaccacctt ggtgccgacc accggcaggc ggacgccgta actgtacttg ttgaccagaa
     1381 tgaagtcgcc cacttcgagc gtcggaatca tcgaaccgga gggaatctgg aacggctcga
     1441 acaggaacga gcgcagcacc agcacgatca gcagcaccgg aaaaaaggat ttcgaatact
     1501 cgacccagac cggttcactc ggcgcggcgg ccaccgatgt cgcctgggcc tggcggcgcg
     1561 cagccagcca gagccggtcg gccagcacca ccagaccggt cacggcggtg ccgaccacca
     1621 gaatcagcgg cagatcaatg ttcatcactt gtccaccttc aacacggcca ggaaggcctc
     1681 ctgtggcacc tcgaccgagc caagctgctt catgcgtttt ttgccttctt tctgtttttc
     1741 aagcagtttg cgcttgcgcg acacatcgcc tccataacat ttggcggtga cattcttgcg
     1801 caacgccttg accgtggtgc gggccagcac ctgtccgccg atcgctgcct gaatcgccac
     1861 atcgaacatc tgccgcggaa tcagctcgcg cagcttctcg gccacctggc gaccgcgctg
     1921 ctgcgcctgc tgccgatgca cgatcaccgc cagcgcatcg accttgtcgc cattgatcag
     1981 gatgtcgagc cgcaccacgc tggccggctc gaaacgctcg aagccgtagt cgagcgaggc
     2041 atagccacgg ctgaccgact tgagccggtc gaagaagtcg agcaccacct cgctcatcgg
     2101 cagttcatag accagcgagg cctggttgcc gtggaattgc agatccttct gcacgccgcg
     2161 cttctcgacg cagaggttga tcacgccgcc gagatactgc tgcggcacca gaatgttggc
     2221 gcggcagatc ggttcgcgca cctccagaat ctggttggga tcgggcagct tggaggggtt
     2281 gtcgaccagc accaccttct gctccttggt caatacctca tagaccaccg tcggcgccgt
     2341 cgtgatcagg ttgaggtcat actcccgctc cagccgctcc tggatgatct ccatgtgcaa
     2401 catgccgaga aaaccgcagc gaaaaccgaa accgagcgcg tccgaactct ccggctcgta
     2461 gaacagcgag gcatcgttca gggtcagctt ggacaatgcc tcacgaaagg cctcgtaatc
     2521 ctcggagttg acggtgaaga gcccggcata gacctgcggc ttgatgcggg cgaaaccggc
     2581 cagcgcctcc acctcgggcg tcgcggcatg ggtgatggtg tcaccgaccg gcgcgccctt
     2641 cacctccttg atgccggcca ccacatagcc gacctcgccg gcgctcagca gtccggtttc
     2701 ggtgcgcttg ggggtgaaga tgccgatgcc ctcggcctgg tgactgcggc ccaccgactt
     2761 gatcagaatc ttgtcgccct ttttgatctg cccctgcatc acccgcacca gcgacaccac
     2821 ccccaggtag ttgtcgaacc aggagtcgat gatcagggcc tgcagcggtg ccgcggcatc
     2881 gccgaccggt ggcggcatca cctcgatcag cttttccagc agctcctcga ccccctgacc
     2941 ggtcttggcg ctgaccagca ccgcctgatg ggtgtcgatg ccgatgatct cctcgatctc
     3001 gcggcagacc cgttcgggat cggcctgcgg caggtcgatc ttgttcagca ccggcagcac
     3061 ctcgagcccc tgctcgatgg cggtgtaaca gttggccacc gattgcgcct cgaccccctg
     3121 tgcggcgtcg accaccagca gtgccccttc gcaggcggcc agcgaacgtg acacctcata
     3181 ggagaagtcg acgtggcccg gcgtgtcgat cagattgagc tgataggtca gcccatcacg
     3241 cgccttgtaa tgcagcgtga cgctctgcgc cttgatggtg atgccgcgct cgcgctcgat
     3301 gtccatcgag tcgagcacct gcgcggacat ctcccgctcg ctgagaccac cgcagatctg
     3361 gatgaagcga tccgccagcg tcgattttcc atggtcgatg tgggcgatga tcgaaaaatt
     3421 gcgtatctgc tgcatgctca cgtcgatcgg ttcaccggcg gtggggttga aggagggatg
     3481 gaccggcaaa cggcccataa aagctgcggg atgctagcaa aggcagggga attttgccat
     3541 gcaaaccgcc ccactcactg ccatcccggt gtggctgaca tggctgaata acggctccgg
     3601 caggtcggac gccgattcag tggagcatga agcggcatcc tctgggacgt caccccgctc
     3661 ccagcagttc gatcgaatag ccgtcggggt ccttgacgaa ggcgagcagg gtcgagccat
     3721 gctgcatcgg tcccggttca cggctgatca cgccgccggc agcgcggatg cgctcgcagg
     3781 cggcgtggac atcctcgacc tcgatcgcga tatggccata gccgttgccc aactcgtaat
     3841 ggtcggtgtc ccagttgtgg gtcagttcga tctggacacc ggggtcatcg tcgaaggcga
     3901 gaaaggccag cgtgaagcgc ccctgcggat actccttgcg gcgcagcagc cgcatgccga
     3961 gcacccgctg atagaaatcg accgagcgat cgaggtcggt gacacgcagc atggtgtgca
     4021 gaatgcgcat ggtttggcgc tccttggtca aagtttgcgg ccacggcgcg cggcgatccg
     4081 cagccgcagc gcattgagct tgatgaaacc gccggcatcg gcctggtcgt aggcgccctg
     4141 gtcatcctcg aaggtggcga tgctggcgtc gaacagcgac tcatccgaag cgcgaccaac
     4201 gacgctgaca ttgcccttgt agagcttcag ccgcaccttg ccgttgacat gctgttgcga
     4261 ctggtcgatc aacgcctgca ggcagagccg ttcgggcgac caccagtagc cgttgtagat
     4321 cagcgaggca tagcgcggca tcagctcatc cttgaggtgg gccacctcgc ggtcgagggt
     4381 gatcgactcg atggcgcggt gcgccttcaa caggatggta ccggccgggg tttcgtaaca
     4441 gccgcgcgac ttcatgccga catagcggtt ctcgacgatg tcggtgcgcc ctaccccatg
     4501 agcacccccc agccggttca gctccagcat cacctcggcg gccgatttgg cgacgccatc
     4561 gatcgccacc acgtcgccat tgacgaaagt gagctcgact tcgaccgcat ggtcgggcgc
     4621 cagctccggc gagacgctcc agcgccacat gccctcttcc ggctcggccc agggatcttc
     4681 gagcacggcg ccttcgtagg agatgtgcag caggttggca tccatcgagt agggcgaggc
     4741 gttgccccgc ttctgctcga cggcgatgcc atgttgttcg cagtaggcga gcagcttttc
     4801 gcgcgagttg agcgtccact cccgccatgg tgcgatcacg cggatgcccg gtttgagtgc
     4861 ataggcaccc agttcgaaac ggacctggtc attgcccttg ccggtggcgc catgggagat
     4921 ggcatcggcg ccggtctcct cggcgatctc gaccagccgg cgggcgatca gcggccgggc
     4981 gatcgaggtg ccgagcaggt actcgccctc gtagagggtg ttggcgcgga acatcggaaa
     5041 aacgtagtcg cgggcaaagg tttcgcgcag atcctcgatg tagatctcct tgatgcccat
     5101 cgcctgggcc ttggcgcggg ccggttcgag ctcttcgccc tgccccaggt cggcggtgaa
     5161 ggtgaccacc tcgcagtggt aggtctcacg cagccagtgg gcaatcaccg aggtgtcgag
     5221 accgccggaa taggccagta ccactttttt gatcgcagac atggggctcc tcaatcaggg
     5281 atgttcaatg ggtgaattca aaggggtcgg catcgagatg ggccggaaaa ccggcccgga
     5341 accgctgcag tgccgccaga tcgagcgtcg cggtcagcag cgcctcttcg ccgggtgcag
     5401 ccgccagcag cggttcaccc agccagtcgt agagtgcgct gtcgccgctg taggcgtggc
     5461 ccttgccatc cagaccgaca cgattgaccg ccgcgacata gctgagattc tcgatcgcgc
     5521 gcgccggcag cagccgttgc caggcactgc tgcgcgctgc cggccagttg gcgacgcaca
     5581 gcagcaggtc gtagtcgtcg cggttgcgac accagaccgg aaagcgcagg tcataacaga
     5641 tgatcggtcg aatcctgaaa ccgttcagtg acatcaccac cgccgattcg ccgctgcggt
     5701 agtggtgatg ttcgccagcc atgcgaaaac agtggcgttt gtcgtagtgg tgcaaggctc
     5761 catcaggggt ggcccagacg aagcggttgt aacagagacc ctgctcctcg atcgccagcg
     5821 aaccgcagac gacggcgttg cgtgcctggg cggtggtgcg cagccaggcg atgccgcgac
     5881 cagccatggg ttcggcctgc tgctgactcg ccatcgtgaa accggtggtg aacatctccg
     5941 gcagcacgat caggtcgacc gccgcgagcg gcgccagcag ctgatcgaag tgggtcaggt
     6001 tggcctcgat gtcatgccag gcgagatcgg cctgcaccag cgccaatgtc agagtcgaca
     6061 tagccgctcc gccgcctgca gcaataccgc atcactcttg gcgaaacaga agcggacgat
     6121 gcgggtcgcc ggtggtgtct gatagaagac cgacaccgga atcaccgcca ccttggcctc
     6181 ccgggtcagc cactgggcga actcgacatc gttcaggtcg acgatggcat cgtaggcggc
     6241 cagttgaaag taggtgcccc ggcacggcag cagctcgaag cggctcggct tcagcgcggc
     6301 ggcaaagagg tcgcgctttt gctggtagaa ggccggcaat ccggccagat gctcgggctg
     6361 ggcgcgcagg gtgtcggcga tggcccactg cgccggcgtg aagctggcga aggtcagaaa
     6421 ctgatgcacc ttgcggaact ccgccgacag cggcggcggc gcgatgcagt aacccagctt
     6481 ccagccggtg atgtgaaaac tcttgccgaa ggaggagatg atgaagctgc gcgccgccag
     6541 ctcggcatgg cggtgaaaac tggcatggtc gaggccatcg aagtggatgt actcatacac
     6601 ctcatcggcg atcagccaga tgtcgcgctc gcgcaccagc gctgccaact gctgcagatc
     6661 ctcgcccgcc agcaccgatc cggtggggtt gtgtggcgaa ttgatgatga tcagccgggt
     6721 tctgtcactg atgcagtcgg ccactcgatt ccagtcgatg cgaaactccg gcgcttcgag
     6781 ggcaatgtgg cgggcagtgc caccgctcaa ttcgattgcc ggttcgtagg agtcataggc
     6841 cggatcgaac aggatcacct catcgccggg acgcaccacg gcggtgatgg cggcatagag
     6901 cgcctcggtg gcgccggagg tgacggtgac ctcctgctcg gcatcgaccc ggcgccgata
     6961 gcggcgcgcc acctgctcgg cgatcgcctc acgcagcgtc gccacgccgg tcatcggtgc
     7021 atactgattg tagccctgct cgacatagtg atgcagccgc tcgcgcagca gtgccggccc
     7081 atcgaaatcg ggaaagccct gcgacagatt gatggccccg cactcggcag ccagcgccga
     7141 catgcgggtg aagatggtgg tgcccacctg gggcagcttg cttggcagtg gccggatcat
     7201 gggcaaggtc atcgctgtct cgatcaaagc ccgtcggcgg tgcgcacggg cggtgcagaa
     7261 catcgggtcg ccgtcggaat gcaggccatg cggcgctgcg gctttcgggc tatcatcgcc
     7321 gccacccgag gcagccaaca cgaggccggc aagcatggca caacctgacg acgtggatca
     7381 actggccgaa cggctggtcg agacggtgcg cttttttgcc gaaaatggcc actgtcccgc
     7441 caccggcggc aacttttcgc tgcgcagcgg cgatcgggtg ctgatcaccg cctccggtgt
     7501 cgacaagcgg agcctgaccc gcgcgcaact gctcggcctc acgctcgatg gccagttgtg
     7561 cgcaccgggc aagccctcgg ccgaaaccga gctgcatctg gcgctctacc gtgccgacgc
     7621 cgacatcggc gcggtgctgc acatccactc cgtcgccgcc accgtgctgt cgcggctgat
     7681 cgacggcgac acgctgccgc tgcacggcta tgagatgcaa aaggcgatca gtggcatcga
     7741 cagccatcag cagacgttgc tgctgccgct ggtcgacaac agccagcaga tggcctgcat
     7801 cgccgagcag atcgaacggc aatggtccac cgcccagcgt gcccatgcgg tgctggtgcg
     7861 tggtcatggc ctctacgcct ggggtcatga tctagcagcg gcccgccgcc acctcgaagg
     7921 gtgggagttc ctgctccagt gtgaactgaa ccggcggctg ctggagcgaa atccatgacg
     7981 ctgcgcgcca tcgtcaccga catcgaaggc accaccaccg acatccactt cgtccaccgg
     8041 gtgctgttcc cctacgctgc cgaacggctc gaagggttca tccgccagca tggcgatgag
     8101 gccgaggtgg ccgccctcct cgaccaggtg cgtgaggaga tccgccagcc gcaggccacg
     8161 gctgccgcat tggtcgacac cctgcaacag tggatcaaac tcgatctgaa gctgaccccg
     8221 ctgaagagcc tgcaagggct gatctggcgc gaaggttacc agcagggcca cttcaccgcc
     8281 cacgtctacc ccgaggtggc cgctgccctg cgcgactggc agcggcgtgg cctggccctc
     8341 tatgtctact cctccggctc ggtcgaggcg cagcgactgc tgttcgccca cacccaggat
     8401 ggcgacctca ccccgctctt cagcggctac ttcgacaccc gcatcggcgc caaacgcgaa
     8461 cgctcgagct atgacagaat tgccgagcag atcggccttg ccggcgacga aatcctgttt
     8521 ctgagcgatg tcgaggccga actggatgcc gctgccgccg ccggttttca cacctgcctg
     8581 ctgctgcgcg atggcgccaa cggcagcggc caccatccat ccgcccacga tttcgagcag
     8641 attccactcc tgcaggagca gccatgagcc acctgacccg ctaccaccag gaccgacccg
     8701 aacagccact cggcaacacg agcgacgccg aggagattcg cgcgctgctg gccgaggccg
     8761 gcgtgcgctt cgagcgctgg cgcgccgacc ggccgctgcc ggccgatgcc gatcaggcac
     8821 agatcggtgc cgcctaccgc agcgagatcg accggctgat gcgcgaaggc ggctaccaat
     8881 cctgggatgt gatctcgatg cggcccgacc accccgaccg gctggcgatg cggcagaaat
     8941 tcctcgacga acacacccat gccgaagacg aagtgcgctt cttcgtcgct ggcgaagggc
     9001 tgttcaacct gcacctcggt gaaaccgttt ttgcgctgct ctgccagcgc gaagacctga
     9061 tcagcgttcc ggccggcacc cgccactggt tcgacatggg cgatcagccc gacttcatcg
     9121 ccattcgcct cttcaccaat cccgaaggct gggtggcgcg gatgaccggc gacgggatcg
     9181 gccgccgctt tccgtccatc gaccactacc gctgagctgt ggtgcgtcat cggcttgtaa
     9241 tgccgcgatg cttggagtct aatctcgccc tttttccttc gagagcggcg taaccatggc
     9301 actttatgtg caaaaatttg gcggcacctc ggttggcacc ctgccccgca tcgaggcggt
     9361 tgccgaaaaa gtggccagct accgcaacca gggacacgat ctggtggtgg tggtctcggc
     9421 catgagcggc gagaccaacc gcctgctggg tctcgcccgg gatctggccg atccggccga
     9481 cccccgcgaa ctcgatgcat tggtctgcac gggtgagcag gtcaccgccg cactgctggc
     9541 catggcgctg atcaaacgcg gctgccccgc ccgctcgatg aacggcgccc aggtgccgat
     9601 tctgaccgac aacgccttca ccaaggcccg catcgagcgc atcgacgacc aggcgctgcg
     9661 cgccgcgctg gcggctggcg aagtggtggt ggtcaccgga tttcaggggg tcgatgccga
     9721 gggcaacgtc accacgctcg gccggggcgg ctccgacacc accgccgtgg cgctggccgc
     9781 cgccctcaag gccgatgagt gccagatcta caccgatgtc gatggcgtct acaccaccga
     9841 tccacgggtg gtcgaacgcg cgcgacggct ggagcggatc accttcgagg agatgctgga
     9901 gatggccagc ctcggctcca aggtgctgca gattcgttcg gtcgaattcg ccggcaagta
     9961 tgccgtcccg ttgcgggtgc tctccagctt cgtggagggc tccggcaccc tgatcaccct
    10021 ggagaacccg aacatgatgg aaaatccgat catctccggc atcgccttca accgcgacga
    10081 agccaaggtc accgtgctcg gcgtgcccga tgtgcccggc gtggccgcac gcatcctctg
    10141 cccgatcagc gacgccaaca tcgaggtcga tgtcatcgtg cagaatgtcg ccgccgacag
    10201 gagcaccgac ttcaccttca ccgtgcaccg caacgacctg cagaaggcgc tggcaatcct
    10261 caacgcccaa gccggagcac tcaatgcccg tgaaatcaag ggcgacagaa gcgtggtcaa
    10321 ggtctcgctg gtcggggtgg gcatgcgctc gcacgccggt atcgccagca agatgtttgc
    10381 tacacttgcc gaggttggga tcaacatcca gatcatcacc acctctgaaa tcaagatttc
    10441 agtggtgatc gacgagaagt acctggaact ggcagttcgc tccctgcaca ccgcattcgg
    10501 gctggacacg cccgaagaga ttgccgtcgt cgaagagggc tgaaagcccc gcccaggcac
    10561 tgggtgcatg gcaccgccct tgagcggaac aagattcaag aacggaacaa cgaggagaaa
    10621 cgcaatgttg atactgaccc gccgggttgg cgaaaccctg atcatcggcg atgacatcac
    10681 cgtcaccgtg ctgggcgtca agggcaacca ggtgcgcctg ggcgtcgacg cccccaagga
    10741 ggtctcggtg caccgcgaag agatctacca gcgcattcag gccgaaaaac ggcaaggcca
    10801 cgacgacgaa tgaggtcgat tcacgctgcc gtgcgtgtta tcatgtcgcc cgcttcgcgg
    10861 cgggtctgac accatcaggc cgcgaatctt caaacctgcc agatcagaac cgcaagccgt
    10921 tcgtccgctg cgctgttcga cgttttccgg agaggtggcc gagaggccga aggcgctccc
    10981 ctgctaaggg agtatgggtc aaaagctcat cgagggttcg aatccctccc tctccgccac
    11041 atcgccatgc caatgcaatc tctttcgctg atcggctgga tcttgccagt ccatcacctg
    11101 atgattccgg caacaccata caaatcatgc tcttgactcg aaatcgggca agctgttctg
    11161 gccaagaacg ggaaaccgtg gtctgtccca gatccttcga aaaccgtctc ttcacgtcca
    11221 acctccgtcg tctgggggat tggact
//
LOCUS       DAORCQ010000075        11137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_3908_length_11137_cov_5.273868, whole genome shotgun sequence.
ACCESSION   DAORCQ010000075 DAORCQ010000000
VERSION     DAORCQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_11137_cov_5.273868"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1754
                     /locus_tag="PKI23_08500"
     CDS             336..1754
                     /locus_tag="PKI23_08500"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNN37017.1"
                     /translation="MPTRTRLSLAIALALASQYAAAASTQFDNFTPLAASSPANPTGS
                     PLETTTPLTLSSPKFKQQVIAQRNTQTGLGQFNSGAWDMITTNETGPDAGRYLFTVFE
                     SGQSGVQRTDTWNNNATQTLWRSPAAGGHVAFDASYWTPWGSFITAEESWTTAPGGAT
                     TSPYGRLYEMTNPLSSNITPNFVHRNVIPRTSHEGIQFDQANHMYFIDELNGGNVYRY
                     SSAAGAGSSYFDAGKTSVLRIGDGNTPNATGAFSWVDITDASGNGLAGTLTITDPNGV
                     VSLDARNTTDLAAFKGTDYQRPEDLQIKRAANGDELLFMTTTTTNEVYTVNLTSGMVS
                     VFADRNTINLHDNLAVGSPDFTSPDNLAMDAAGNIYIIEDQPGGMADIWFAKDTNHDG
                     DLNDAGEGLALWASMNTNGAEPTGLYFDPFNPNRAWLNIQHPASGNDVMVELTAVPLP
                     AALPLSLSALAALGLVARRRTA"
     gene            1910..2374
                     /locus_tag="PKI23_08505"
     CDS             1910..2374
                     /locus_tag="PKI23_08505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNN37018.1"
                     /translation="MSQLEIGTVIPPFSVQSTAGEITPRMLHGRQWVLYFYPKDATPG
                     CTSEGQDFRDLHPEFRACNTELFGVSRDSLASHERFRQKQGFPFALISDSDETLCRLF
                     DVIQEKNMYGRKVLGIERSTFLIDAEGRLARIWRKVKVKGHAAEVLAACGGR"
     gene            2513..3730
                     /locus_tag="PKI23_08510"
     CDS             2513..3730
                     /locus_tag="PKI23_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37019.1"
                     /translation="MSTESAELREFRAQVHNWLKSNRPPKPSFPLPQSFTEVGNNDQF
                     EYLRDWQQKVYEAGYLGMAWPKQYGGGGMPQAYQDIVTEEMYRLRTPLMVNAVGLSWA
                     GPTILKHGTEAQKKKYIRPILEASEIWCQGFSEPDHGSDLGNVQTRAVRDEASGDYII
                     NGRKIWTTLGEQAKHIILLARTNPNAPNKYAGLSFFLAPMDVPGVEVLPIKKITHEYG
                     FNETVFTDVRIPAESMMGNEGEGWFIAMTVLTFERGVTGGQAGGNMMEIKNVHDIHAL
                     GRGLFRDGKLALQDPSLRAELIDLYITERAHKLNKLREDFPVLQTPERTGGLGLGNKL
                     VMSEDRHQMAQFAVRMLGNQTLLYADDPDVTADGLWQHARLAAFAGSIGGGTTEVQRN
                     IIAERVLRLPKSR"
     gene            3787..4944
                     /locus_tag="PKI23_08515"
     CDS             3787..4944
                     /locus_tag="PKI23_08515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37020.1"
                     /translation="MNLSETELNFQCSEELGMLTDAATSYLRENSTIADARRLAETDS
                     GYSPEQWQTLAEMGWLGLATPEEFGGSGLGYVELTKISELMGRYLFSSPFLATTLASQ
                     ALIASHDEDKKMQWLPELSSGSSIATLALMEQNNSWELGDITCTADSSGDGYRLDGIK
                     QFVLDGQNADLMLVTALLKGRPALFLLTPDLVKKLVQRPEQLTHHARRSSRFRFGGLQ
                     LPKSALLAEGDEARRILGHVHSLAWLLLAADHAGTANGAIELTAEYLRVRKQFGKYIG
                     TYQALSHPLVNAAMLTEDTLSLAYHGAALFDGTLGGQSEISARMAKVRGGEASTLAGS
                     RAIQSHGGMGFTWECHAHLYFKRGQWNEYSFGDGGHQKRHLADLIIGDLPH"
     gene            5064..5600
                     /locus_tag="PKI23_08520"
     CDS             5064..5600
                     /locus_tag="PKI23_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HNN37021.1"
                     /translation="MKPSPRLIPLHSDSGTDLSPDEQAAVTVALDGQCDQSALLPILH
                     AVQDALGYVPSAAVPLIAHQLNLSRAEVHGVVSFYHWFRQQPPGRHVLHLCRAEACQA
                     VGGRALEAHVMRTLAVEFGQTSADGAVTLEPVYCLGNCALGPSLMVDGTLHGRVTASR
                     FDRLLRQLRDDADEAAAP"
     gene            5597..7153
                     /locus_tag="PKI23_08525"
     CDS             5597..7153
                     /locus_tag="PKI23_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNN37022.1"
                     /translation="MTVRLYLPGDASALSVGAEQTAQALIAEARQRGSEIELIRNGSR
                     GLFWLEPLLEVVTPGGRLAYGPVAAEDVAGLFDAGLLQGGHHPLALGPTEEIPWLKRQ
                     RRLTCARIGLIDPASLSDFVAHGGYRGLQQAFAIGPEETLKTVADSGLRGRGGAAFAT
                     GRKWRTVAETPATQKYIVCNADEGDSGTFADRLLLEGDPFALIEGMTIAGFATGATEG
                     YIYLRCEYPHAARALRKAIATARSEGHLGENILGSGHHFDLQLRLGAGAYICGEESAL
                     LESLEGRRGQVRFRPPLPAVKGLFGAPTVVNNVITLASVPTILAEGADSYRALGSGQS
                     RGTLPIQLGGNIRHGGLIELPFGVTLNELLHDHGGGSASGRPIRAVQVGGPLGAYHPV
                     SQFDTAIDYEAFAAIGGMLGHGGIVVFDDSVDLAEQARHAMAFCAHESCGKCTPCRIG
                     STRGLEVIDRLRAGQEPLAQQALLRDLCHTLTHASLCGLGSLTPYPVLSALDHFPEDF
                     QPRPVGSTVQ"
     gene            7161..9935
                     /gene="fdhF"
                     /locus_tag="PKI23_08530"
                     /pseudo
     CDS             7161..9935
                     /gene="fdhF"
                     /locus_tag="PKI23_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            9950..10807
                     /gene="fdhD"
                     /locus_tag="PKI23_08535"
     CDS             9950..10807
                     /gene="fdhD"
                     /locus_tag="PKI23_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNN37023.1"
                     /translation="MESSTPPAGTAAVTVTTVQDGRISRAADLLATEVPVALHCNGSP
                     FVVMMATPHQLDDFAHGFSVSEGLVDSIDEITACAVRPIEPGRPLAGFEIALQIPPAR
                     HAALLKRQRNLLGRSSCGLCGTATIEAAMHSPRPVTSSMTLPTGAIDRALAALAHHQP
                     LNQLTGALHGAAWCTITGEITALHEDVGRHNALDKLIGQRLRARDDFAAGFALITSRA
                     SYEMVQKSASVGIAVLVAVSAPTLAAVELATACNITLVGFARGNRHLIYSHPQRLLAS
                     ADSGRVAAR"
     gene            10804..11049
                     /locus_tag="PKI23_08540"
     CDS             10804..11049
                     /locus_tag="PKI23_08540"
                     /inference="COORDINATES: protein motif:HMM:NF022832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HNN37024.1"
                     /translation="MSGQQLVEMANDIATFFAAEPDPAVATLGIESHLRKFWSPRMRT
                     KLIEQWRRDDSALTPLAAAAVARLAQTEQIDEDQPQQ"
BASE COUNT         2058 a         3818 c         3382 g         1879 t
ORIGIN      
        1 cggttcgtgc tgtttcacga caaacgccac ccgcgggaga tgggtgcagc cgagaagaat
       61 tggaagaatt tgggacagac tgagctatcc ccaattcccc tacttttatc gacagtgagc
      121 agtggagcac cgctgccacg ctgaaaattc atcctcccgt caaccgggcg tcacaattgc
      181 cgtctaccgt gcgtccgtga agggagaaca gccagcttcg acccgccatc gagtggtctc
      241 ttctcttccg caacgaatgg attcggccgt cggccggatg ccattcatcc tgatggccca
      301 taaccattcg acgaacacgc acaggagaat ccgtcatgcc aacgcgcacc agactttcgc
      361 ttgccatcgc tctggccctc gccagccagt atgccgcagc cgcttccacc caattcgaca
      421 acttcacgcc gctggctgcc tccagcccgg ccaaccccac cggctcaccg ctggagacca
      481 ccactccgct gaccctctcc agcccaaaat tcaaacaaca ggtgatcgcc cagcgcaaca
      541 cccagaccgg ccttggccag ttcaacagcg gcgcctggga catgatcacc accaatgaaa
      601 ccggcccgga tgccggccgc tacctgttca ccgtattcga aagcggccag tcgggcgtgc
      661 agcgcaccga cacctggaac aacaatgcca cccagaccct ctggcgcagc ccggcggccg
      721 gcggccatgt cgcttttgat gcctcctact ggacaccctg gggttcgttc atcaccgccg
      781 aggagagctg gaccaccgcg cccggcggcg ccaccaccag cccctatggc cgtctttatg
      841 agatgaccaa tccgctcagc agcaacatca cgccgaactt cgtgcaccgc aacgtgattc
      901 cccgcacttc gcatgaaggc atccagttcg accaggcgaa ccatatgtac ttcatcgatg
      961 agctgaatgg cggcaatgtc tatcgctaca gctcagcggc gggtgccggt tccagctact
     1021 tcgatgccgg caagacttcg gtactgcgca tcggtgatgg caacaccccc aatgccaccg
     1081 gtgcgttcag ctgggtcgac atcaccgatg ccagcggcaa cggcctggcc ggcaccctga
     1141 ccatcaccga ccccaacggt gtcgtttcgc tcgacgcgcg caacaccacc gacctggctg
     1201 ccttcaaggg caccgactat cagcgcccgg aagatctgca gatcaagcgg gcggccaacg
     1261 gcgatgagct gctgttcatg accaccacca ccaccaacga agtctatacg gtcaacctga
     1321 cctcgggcat ggtctcggtc tttgccgatc gcaacaccat caacctgcat gacaatctgg
     1381 cggtgggctc tcctgacttc accagtcccg acaacctggc gatggatgcc gctggcaaca
     1441 tctacatcat cgaggaccaa cccggcggca tggccgacat ctggtttgcc aaggacacca
     1501 accacgatgg cgacctgaat gatgcaggcg aaggcttggc gctctgggcg tcgatgaaca
     1561 ccaacggcgc cgaaccgacc ggcctctact tcgatccgtt caacccgaac cgtgcctggc
     1621 tcaacatcca gcatccggcg agcggcaacg atgtgatggt cgagctgacc gcggtaccgc
     1681 tgcctgccgc actgccgctg tcgctgtcgg cactggcagc gctcggtctg gtggcacgtc
     1741 gccgcacggc ctgatcgact gacagccggc agcactgctg ccctgggacc cccgctccat
     1801 ggcgggggtc ttttttctga aagcccctgc tcccattcct cgacctgctg tgttcaactt
     1861 gccatgtcct accctgttga actctttgca caccggagca gaagccaaga tgagtcagct
     1921 cgaaatcggc acggtcattc ccccgttcag cgtccagtcc acagccggag agatcacgcc
     1981 acggatgctg catggccggc agtgggtgct ctacttctat ccgaaggatg ccacccccgg
     2041 ctgcaccagc gagggacagg atttccgcga cctccatcca gagttccgcg cctgcaacac
     2101 cgaactgttc ggtgtctcgc gcgattcgct cgccagccat gaacgattcc ggcagaagca
     2161 ggggttcccg ttcgcgttga tcagcgacag cgacgaaacc ctctgccggc tgttcgatgt
     2221 gattcaggaa aagaacatgt atggccgcaa ggtgctcggc atcgaacgct ccaccttcct
     2281 gatcgatgcc gagggtcggc tggcacgcat ctggcgcaag gtgaaggtca agggccatgc
     2341 cgccgaagtg ctcgccgcct gcggcggccg atgagcgcct gctgcgccgg tcgggtcaag
     2401 agtgatatat tcgcgcgcgg cgtccagacg ctgaacagaa gataaaaaca ccacaccaag
     2461 cacgccaggg ccggcatcca caaccggcag gtgctcgaca aggagaacca tgatgtcaac
     2521 cgaatcggcc gagttgcgcg aattccgcgc ccaagtccac aactggctca agagcaaccg
     2581 gccacccaag ccgagctttc cgctgccgca atcctttacc gaggtgggca acaacgacca
     2641 gttcgaatac ctgcgcgact ggcagcagaa ggtctatgag gcgggctacc tcggcatggc
     2701 ctggcccaag cagtacggcg gtggcggcat gccacaggcc tatcaggaca tcgtcaccga
     2761 agagatgtac cggctgcgca cgccactgat ggtcaatgca gtggggctgt catgggccgg
     2821 cccgaccatt ctcaagcatg gcaccgaggc gcagaagaag aagtacatcc ggccgattct
     2881 ggaggccagc gaaatctggt gtcagggttt ctccgaaccc gatcacggct ccgacctcgg
     2941 caatgtgcag acccgtgccg tgcgcgacga ggccagcggc gactacatca tcaatggccg
     3001 caagatctgg accacgctgg gtgagcaggc caagcacatc attctgctgg cgcgcaccaa
     3061 ccccaatgcg ccgaacaagt atgccggcct cagcttcttt ctggcgccga tggatgttcc
     3121 cggcgtcgag gtgctgccga tcaagaagat cacccatgag tacggcttca acgagaccgt
     3181 cttcaccgac gtgcgcatcc cggccgagag catgatgggc aacgagggcg agggctggtt
     3241 catcgccatg acggtgctga ccttcgagcg cggcgtgacc ggcggtcagg ccggcggcaa
     3301 catgatggag atcaagaacg tgcatgacat ccatgcactg ggccgcgggc tgttccgcga
     3361 tggcaagctg gcactgcaag acccgtcgct gcgggccgag ctgatcgatc tctacatcac
     3421 cgagcgggca cacaagctga acaagctccg tgaggatttc ccggtactgc aaacgccaga
     3481 gcggaccggc gggcttggcc tcggcaacaa gctggtgatg tcggaagatc gccaccagat
     3541 ggcgcagttc gcggtgcgca tgctgggcaa ccagacgctg ctctacgccg atgatcccga
     3601 tgtgacggcc gatggcctgt ggcagcatgc ccggctggcc gccttcgccg gttcgattgg
     3661 cggcggcacc accgaagtgc agcgcaacat catcgccgag cgggtgcttc gcctgcccaa
     3721 atcgcgctga gcgcggccac actgccaccc gtgcgttgat acctcattga taaaaaggtc
     3781 aaggacatga atctttccga aacagaattg aatttccaat gcagcgaaga gctggggatg
     3841 ctgaccgacg ccgccaccag ctacctgcgc gagaactcca ccatcgccga cgcccgccgt
     3901 ctggccgaga ccgacagcgg ctactctccc gagcagtggc agacgctggc cgaaatgggc
     3961 tggctggggc tggccacgcc ggaagagttc ggcggcagcg gcctgggcta tgtcgagctg
     4021 accaagatca gcgagctgat gggccgttac ctcttctcct ccccgttcct ggccaccacg
     4081 ctggccagcc aggcgctgat cgccagccat gatgaagaca agaagatgca gtggctgccg
     4141 gagctcagca gtggcagcag cattgccacg ctggcactga tggagcagaa caactcctgg
     4201 gagctgggcg acatcacctg caccgccgac agcagtggcg acggctaccg cctcgacggc
     4261 atcaagcagt tcgtcctcga tggccagaat gccgacctga tgctggtgac cgcactgctg
     4321 aagggccgac cggcgctgtt cctgctgacc cctgatctgg tgaaaaagct cgttcagcgt
     4381 ccggaacagt tgacccacca cgcccgccgc agcagccgct tcaggttcgg tggcctgcaa
     4441 ctgccgaaat cggccctgct ggccgagggg gacgaggcca ggcggattct cggccatgtt
     4501 cactcgctgg cctggctgct gctggccgcc gaccatgccg gcaccgccaa cggcgccatc
     4561 gagctgaccg ccgaatacct gcgggtgcgc aagcagttcg gcaaatacat cggcacttac
     4621 caggcgctct ctcacccgct ggtcaacgcg gcgatgctga ccgaagacac cctctcgctc
     4681 gcctaccatg gcgctgcact gttcgatggc accctgggtg ggcagtcgga gatctccgcc
     4741 cgcatggcca aggtgcgcgg tggcgaggcc agcacgctgg ccggctcacg ggcgatccag
     4801 tcgcatggcg gcatgggctt cacctgggag tgtcacgccc acctctactt caagcgtgga
     4861 cagtggaacg agtacagctt tggcgatggc ggccaccaga agcgccatct tgccgatctg
     4921 atcatcggtg acctgccgca ctgatctggc cctccatggc tgggccggag cgttccggtt
     4981 cagccatgga gccgatgttc gagcttggca gctcgattgc ttcatgaaga gaggaatgac
     5041 cgggactttc aagggcaacc cgcatgaaac cgtcaccacg gctgattcct ctgcattccg
     5101 actctggcac tgatctttcc cctgacgaac aggccgcagt cacggtggcc ctcgacggcc
     5161 agtgcgacca atcggcgctg ctgccgatcc tccacgcagt gcaggatgcg ctcggttatg
     5221 tgcccagcgc cgccgtcccg ctgatcgccc accagctcaa cctctcccgc gccgaagtgc
     5281 atggcgtggt cagcttttat cactggtttc gccagcagcc accgggccgg catgtgctcc
     5341 acctctgccg cgccgaggcg tgtcaggcgg tgggcggccg cgcactcgaa gcccatgtca
     5401 tgcgcaccct cgccgtcgag ttcggccaga ccagcgccga cggcgcagtc acgctggagc
     5461 cggtctactg cctgggcaac tgcgcgctgg gcccctcact gatggtcgat ggcacactgc
     5521 atggccgcgt gacggccagc cgcttcgatc gactgctgcg ccagctgcgc gacgacgccg
     5581 acgaggccgc agcaccatga ctgtccggct ctatctgccc ggtgatgcct cggcactctc
     5641 ggtcggcgcc gagcagaccg cccaggcgct catcgccgag gcgcgacagc gtggcagcga
     5701 gatcgaactg atccgcaacg gctcgcgcgg cctgttctgg ctggagccgc tgctcgaggt
     5761 ggtcacgccc ggcgggcggc tcgcctatgg cccggtggcg gccgaggatg tcgcgggact
     5821 gttcgatgcc ggcctgctgc agggcggcca ccatccgctg gccttggggc cgaccgaaga
     5881 gattccctgg ctgaagcggc agcggcggct cacctgtgcc cgcatcggtc tgatcgatcc
     5941 agccagcctc agcgattttg tcgcccatgg cggttaccgg ggtctgcaac aggcttttgc
     6001 gatcggcccc gaagagacac tgaaaaccgt cgccgactcc ggtctgcgcg gccgtggcgg
     6061 cgcggccttt gccaccggcc gcaagtggcg gaccgtggcc gaaacgccgg ccacccagaa
     6121 atacatcgtc tgcaatgccg acgaaggcga ctccggcacc tttgccgacc gcctgctgct
     6181 ggagggtgac cccttcgcgc tgatcgaagg catgaccatc gccggcttcg ccactggcgc
     6241 aaccgagggc tacatctatc tgcgctgcga atacccgcac gccgcccgtg cgctgcgcaa
     6301 ggcgatcgcc accgcgcgca gcgagggcca tctgggcgag aacatcctcg gcagcggcca
     6361 ccacttcgac ctgcaactgc gtctcggtgc cggtgcctac atctgcggcg aagagagcgc
     6421 actgctcgag agcctggagg gacgccgcgg tcaggtgcgc tttcgcccgc cgctgccggc
     6481 agtcaaggga ctgtttggtg cacccaccgt cgtcaacaac gtcatcaccc tcgcctcggt
     6541 gccgaccatt cttgccgagg gtgccgacag ctaccgcgcc ctcggcagcg gccagtcgcg
     6601 tggcacgctg ccgattcaac tgggcggcaa cattcgtcat gggggactga tcgaactgcc
     6661 cttcggcgtg accctgaacg agctgctcca cgaccatggc ggcggcagcg ccagtggtcg
     6721 gccgatccgc gcggtgcagg tgggtggccc gttgggcgcc taccatccgg tcagccagtt
     6781 cgacaccgcc atcgactacg aagccttcgc ggcgatcggc ggcatgctcg gccatggcgg
     6841 catcgtggtg ttcgatgaca gcgtcgacct ggccgaacag gcgcgtcatg ccatggcgtt
     6901 ctgcgcccat gagtcctgtg gcaagtgcac gccctgccgc atcggttcga cccgtggcct
     6961 cgaggtgatc gaccgcctgc gtgccgggca ggagccgctg gcgcagcagg cactgctgcg
     7021 cgacctctgc cacaccctga cccacgcttc gctctgtggc ctcggcagcc tcacccccta
     7081 tccggtgctg agtgccctcg accactttcc cgaagatttc caaccgcgac cggttggctc
     7141 cactgtgcag tgaggtcgcc atgcgttcga tcgccgaaac cgacctggga acccccgcca
     7201 gcccttcgac cgaacagatc agcctgacca tcgatggcca gccggtgacc gttccggccg
     7261 gcagctcggt gctgcgcgcc gccgccagcc tcggcacgca aatcccccgg ctctgcgcca
     7321 ccgatcaact ggagcccttc ggctcgtgcc ggctctgtct ggtgcagatc gatggcatga
     7381 agggctatcc cgcctcctgc accacccagg tggccgaagg gatgcaggtg accagccaga
     7441 gtgccgaact ggcccgactg cggcgcgggg tgatggagct ctacatctcc gatcatccgc
     7501 tcgactgcct cacctgcccg gccaacggcc actgcgaact gcaacagatg gccggtgcgg
     7561 tgggtctgcg cgaggtgcgc tatggccacg acggcgccaa ccatctcgct ctggccaagg
     7621 atgagagcaa cccctacttc agctttgatc cggccaagtg catcctctgt tcacgctgcg
     7681 tgcgtgcctg cgatgaacag cagggcacat tggcactgac catcgacggc cgcggtttcg
     7741 acgcccaggt ggtggccagt cagaaggcca cctttctcga ttcggagtgc gtctcctgcg
     7801 gtgcctgtgt gcaggcctgc ccgaccgcga ccctgagcga aaaatcgctg atcgagctgg
     7861 gacaggccga acacagcgtc gtcaccacct gtgcctactg cggcgtgggc tgttcgttcc
     7921 gtgccgagat gcgtggcgag acggtggtgc gcatggtgcc ggacagccgg ggcgccgcca
     7981 accatggcca cgcctgcgtc aagggccgct tcgccatcgg ttacgccacc caccccgacc
     8041 gcatcacccg gccgctgctg cgcgagaaaa tcagcgaccc gtggcgcgaa gtgagctggg
     8101 aagaggcgat tgccgaggcc gccgcccgct ttcgttcgat ccaggcccgc catggccgcg
     8161 atgccatcgg cggcatcacc tcgtcgcgct gcagcaatga agagacctat ctggtgcaga
     8221 agctggtgcg ggccggtttc ggcaacaaca acgtcgacac ctgtgcccgg gtctgccacg
     8281 cgccgaccgg ttacgggctc aagcagacac tgggagaatc ggccggcacc cagaacttcg
     8341 attcggtgat gcaggccgac gtgattctga tcatcggcgc caacccgacc gatggccatc
     8401 cggtcttcgc ctcgcaactg aagcggcggc tgcgccaggg cgcccgactg attgtggccg
     8461 atccgcgccg gatcgatctg gtgcgctcgc cgcacatcga ggccgcctgg catctgcaac
     8521 tgcgccccgg caccaatgtc gcgctgatca acgcactggc ccatgtcatc gtcaccgaat
     8581 cactggtcga cgaggccttt gtgcaacagc gttgtgacat gcagtcattc gagcgctggc
     8641 gccggttcgt cgccgacgat cggcactcgc ctgaagccac cgaatcgatc accggcgtgc
     8701 cggctgccag cctgcgcgcc gctgcgcggc tctatgccag cggtggcaac agcgccatct
     8761 actacgggct gggggtgacc gagcacagcc agggctcgac gatggtgatg ggcattgcca
     8821 atctggcgat ggccgccgga cagctcggcc gtgccggggt cggcgtcaac ccgctgcggg
     8881 gacagaacaa cgtccagggc gcctgtgaca tgggctcctt tccgcatgag tttccgggct
     8941 atcgccatgt ctccgatgcg cagacacgcc aggcattcga aagtgcctgg ggcgtgccgc
     9001 tgcaaggcga acccggattg cgcctgccca acatgttcga ggcggccatc gatggcgaat
     9061 tcttcggcct ctacatccag ggcgaggaca tcgcccagtc ggaacccgac agccagcgtg
     9121 ttgccaatgc gttgcgcgcg atggagtgcg tcgtggtgca ggatctgttt ctgaaccaga
     9181 ccagccagtt cgcccacatc tttctgcccg gttcgtcgtt cctcgaaaag gagggcacct
     9241 tcaccaatgc cgagcggcgc atctcgcggg tgcgccaggt gatgcggccc agggccggcc
     9301 atgccgactg gcagatcacc cagatgctgt cgaatgcact cggccattcg atgcactacg
     9361 gccatccgtc cgagatcatg gccgaaatcg ccagtctgac cccgagtttt tccggcgtca
     9421 gctatgaaaa actcgaccgt ctgggcagca tccagtggcc ctgtgacgcc cgccatcccg
     9481 atggaacagt ggtgatgcac agcgaaacat tcacccgtgg cctcgggcag ttcatggtca
     9541 ccgagtatgt gccgaccgag gagcgggtca atgccgaata tccgctgatt ctgaccaccg
     9601 gccgcatcct gtcgcagtac aacgtcggca cccagacccg ccgcaccgcc aacctgctct
     9661 ggcatgacca ggatctcgtc gagatccatc cgcacgatgc cgaagagcgc ggcatcggcg
     9721 cgaacgactg ggtgcggctg cagagccgct ccggcgccac gctgatgcgc gttcagatca
     9781 ccgaccgggt gccacccggc gtggtctata ccacctttca ctttcccgag tcgggcgcca
     9841 acatcctcac caccgagaac tccgactggg ccaccaactg tccggaatac aaggtgacgg
     9901 ccgtgcagct gacccgcgtc aagccgccag cccaggcggt gccgcagtga tggagagctc
     9961 gactccaccc gccggcaccg ctgcggtcac ggtgaccacc gtgcaggatg gccggatcag
    10021 ccgtgcagcc gatctgctcg ccaccgaggt gccggtggcg ctccactgca atggctctcc
    10081 tttcgtggtg atgatggcga caccacacca gctcgacgac ttcgcccacg gcttttcggt
    10141 cagcgaaggg ttggtcgact cgatcgacga gatcactgcc tgcgcagtcc gtcccatcga
    10201 acccggacgc ccgctggccg gcttcgagat cgccctgcaa attccaccgg cacgccatgc
    10261 cgcgctgctg aagcggcagc gcaacctgct cggtcgcagc agttgcgggc tctgcggcac
    10321 cgccaccatc gaggccgcca tgcactcacc gcggccagtg accagctcca tgacgctgcc
    10381 gaccggtgcc atcgaccggg cgcttgcggc gctcgcgcat catcaaccgc tgaaccagct
    10441 gaccggcgcg ttgcatggcg ccgcctggtg cacgatcacc ggcgaaatca ctgccctgca
    10501 cgaggatgtc ggccgccaca atgcgctcga caagctgatt ggccagcgac tgcgcgcacg
    10561 ggatgatttc gccgccggct tcgcgctgat caccagccgt gccagctatg agatggtgca
    10621 gaaatctgcc agcgtcggaa ttgccgttct ggtcgccgtc tcggcaccca ccctggcagc
    10681 ggttgaactc gccacagcct gcaacatcac gctggtcggc tttgcccgtg gcaaccgcca
    10741 tctgatctac agccatccgc agcggttgct ggccagcgcg gactccgggc gggtggccgc
    10801 acgatgagtg ggcagcagct ggtagagatg gccaatgaca tcgccacctt ctttgcggcc
    10861 gagcccgatc cggccgtggc cacactgggc atcgagagcc acctgcgcaa gttctggtcg
    10921 ccacggatgc gcacgaagct gatcgaacag tggcggcgcg atgacagcgc actgacgcca
    10981 ctggccgcag cagcggtggc acgactggcg cagaccgagc agatcgacga ggatcagccg
    11041 cagcagtaga gatccctacc cgatcgagcc tgcgccccgc tgctgcacat gcagtttggc
    11101 gccgatgaag tcgcggtgtg agacgtagag caattcg
//
LOCUS       DAORCQ010000076        10959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4010_length_10959_cov_6.276871, whole genome shotgun sequence.
ACCESSION   DAORCQ010000076 DAORCQ010000000
VERSION     DAORCQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_10959_cov_6.276871"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKI23_08545"
     CDS             complement(<1..439)
                     /locus_tag="PKI23_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNN37025.1"
                     /translation="MNTQYRKALPGTSLDYFDAREAVNAIQPGAWDRLPYTARIHAEN
                     IVRKADPAIIDECLKQIIEVKRERDFPWFPARVVCHDILGQTALVDLAGLRDAIAEQG
                     GDPAKVNPVVPVQLIVDHSLAVECGGFDPQAFEKNRAIEDRRNE"
     gene            989..4192
                     /locus_tag="PKI23_08550"
     CDS             989..4192
                     /locus_tag="PKI23_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNN37026.1"
                     /translation="MLGLVRIALQRPYTFIVVALLLLIIGPLAALRTPTDILPAIRIP
                     VIATAWQYTGLPPDQMAGRMITQFQRTLSTTVNDIEHMEANSYAGYGIIKVFFQPGVD
                     LAIANAQVTAASQVGIRQMPAGTTPPLILNYEASTVPIIQLALSGKGMSEQSLADIGL
                     NSVRIGLITVPGLAIPYPYGGKSRQVLIDLDPTALQARGLSAQDVANALAAQNLIVPI
                     GKQKIGEFEYTMQLNSAPDAIAELGNLPIRVVNGAMVYIRDVAQVRDGNPPQSNIVHV
                     DGNRSVLMSVLKNGSTSTLAIVDGVKRKLAEITPSLPENLKVELINDQSLFVRAAITG
                     VAVEGVIAAVLTSLMILLFLGSWRATLIIALSIPLSVLGAIIALSLTGETFNIMTLGG
                     LALAIGILVDDATVTIENINWHLEQGKLVETAIMDGARQIVTPAFVSLLCICIVFVPM
                     FFLEGVARFLFVPMAKSVIFAMICSFLLSRTLVPTLANHLLRTHASHTDLHGNDLALP
                     PSRNPLVRFQRRFEAGFERFRDLYRQLLLLALANRRRFVGGFMLLVLASFALVPFLGR
                     NFFPAVDSGQILIHARVPVGTRVEETAAQFARIQAAIRRIIPADEIATLVDNIGLPIS
                     SINLTYNNTGVMGSQDGDIQIALKPGHAPTADHVRALREQLPRLFPGVTFSFPPADIV
                     SQILNFGAPAPIDVQIRGNNLDANFDHANLLLSKIRRIPGIADARIQQSRSNPVFRVD
                     LDRTQAQQVGLTTRDVTNSLLVNLAGSSQVAPTFWLNPVNGVSYPIVMQTPQHQLDSL
                     STLANLPISGPGNSSPQILGALASIERSRSNALVSQYDIQTMVQIHATTQGRDLGAVA
                     KEVRRVIDENAHARPKGSTVVLLGQVNTMELSYSGLLFGLLGAVVLIYLLIVINFQSW
                     SDPFVIICALPAALAGIVWMLFVTYTPLSVPALTGAIMCMGVATANSVLVISFARERL
                     LELGDATAAALDAGFIRFRPVLMTALAMIIGMAPMALGLGEGGEQNAPLGRAVIGGLI
                     FATLATLIFVPVVFSLVHAGAGHDRMPEAATGEAHAR"
     gene            4182..5396
                     /locus_tag="PKI23_08555"
     CDS             4182..5396
                     /locus_tag="PKI23_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNN37027.1"
                     /translation="MPVDPSTPTLSRTRLRLAALMLLLLAATLVVIGILSRANAEAEL
                     KAATEAQAIPTVAVLLPIKSKQASEIELPGRLEAHARAPIHARVAGYLKSWKVDIGSE
                     VKAGQLLAEIETPELDQQLLQARADLASAQANATMAQKSARRWQAMVGSDAVSQQEAD
                     EKSNDLMVKQSVVKALQANVERMEVLKGFARIQAPFAGTVTARSTDVGALINPGSSGG
                     AELFVIADTRRLRLYVSVPQNQTAKIGPGTEAQVTVPERPGKRYPAKVEASAQAVQAA
                     SGTVLMQLSVDNSAGELLPGSYARVSLALPQAAEGLNIPVSALLFDKSGLRVATVDAE
                     NRVRLKTVTLLRDLGKSIDIGTGLTASDRVIESPPDGINEGDPVQIAAPENKSPAEGK
                     PATDGKRDAGKG"
     gene            5413..6822
                     /locus_tag="PKI23_08560"
     CDS             5413..6822
                     /locus_tag="PKI23_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNN37028.1"
                     /translation="MLALTLALAGCSLAPPPQLPEVPVAASYREEAPWTPAQPADQLP
                     RDGWWQLYGDAELDALQQRLLANSPDLAAALARYRQAEAVTRQSESALLPRLNGSATV
                     QRLQQSQMRPLRPPPAPNAADQYNSNTLGVSADYEVDLWGRISNQVTSSQSTQQAVAN
                     DLASARLSLAAQLADGYLALRGLDRESAFLQQSVESYVKALELTQTRHQGGIASGLDV
                     ARAQTLLDNARSQLAQSRAQRALVEHAIAALVGESASSFSIAPRLTEVRVPPVPVELP
                     STLLQRRPDIAAAQRRVAAANANVGVVRAGYYPAIMLGASAGYQSSDASDWIKAPNLF
                     WSVGPSLVVNLFDAGKRRAEEAQSRALLDEAGAKYRGVVLTAFQQVEDNLALLNHYQS
                     AREAEQSAVAAAQRSVQLAQSRYREGVANYLEVVTAQVAALQSERNALNLAVRQQRAS
                     VQLIRALGGGWSESEAPSP"
     gene            6889..8082
                     /locus_tag="PKI23_08565"
     CDS             6889..8082
                     /locus_tag="PKI23_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN37029.1"
                     /translation="MSNPAVAAPPETLLNRPFLLVLMVAAVFGFSFSSFFLLPKFLSV
                     ALAADAVSIGSITTLFWLASALTVPYIGGLIDRHGRKRYALIGALILALSCIGFVWVD
                     SLGPLIWLLRILQGIGFTLFLVALSTLAVDLAPPARLGQALGIFGAVMILTNAAGPAF
                     AEWTSAHYGWQPVFVATAAAALLAALLCRLLPEQRLLLADTRHATLWQVLRRPGIGPI
                     ILVSAMVGWVFGTLFTFYQPWALSLGIEQVASYFIAYAVVAVTIRIFFGDLPDRLGRL
                     RVVRASLLLYVLTPLALVWLDQLNLYVAGAILGLTQGVYYPALNAVAIDCATPQERGK
                     VMAAYSGAFNFGLAAAGLILGHLVLAMGYRSVFAFASVISLVAFALLAVTPQRHFRPA
                     PMTPE"
     gene            8184..8777
                     /locus_tag="PKI23_08570"
     CDS             8184..8777
                     /locus_tag="PKI23_08570"
                     /inference="COORDINATES: protein motif:HMM:NF036398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37030.1"
                     /translation="MTEQKPGAPWWQTLPGVLTATGTLIVAVTGLLGMLHQSGLLGGG
                     STAGKQPATVEASAAAPSAAQPRPAAPADSDEETDRLDTAEKRPTTPSTANGEPAVTA
                     GLTAAAQQAEFRRRVQQGLYPSEVEGQCKEGVETFRTLWKPLPAGAEFVSVRSMSKEG
                     FQTKRDKFAGDGYAIESVHSYQNCKGVERYHATWIKR"
     gene            8917..10872
                     /locus_tag="PKI23_08575"
     CDS             8917..10872
                     /locus_tag="PKI23_08575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNN37031.1"
                     /translation="MSTRHYRPEIDGLRALAVVPVILFHAGLPAFSGGFVGVDIFFVI
                     SGYLITSIVIKELDQGRFSFGNFYERRARRILPALLLVMLCSIPFSWRWMMPDELKDF
                     AQSLLAVLLFSSNLLFWQESGYFDGEADLKPLLHTWSLGVEEQFYLLFPLSAWLAWRC
                     FRRHVSLFYVALLLVSLLASQWRLAGDASAAFYLLPFRAWELLLGALLALHEARAGGR
                     TAPRWSEWSGAAGLVLILLSLALFGRETPFPGVAALLPTLGTLLVIHGASPATLVGKL
                     LGQRLLVGIGLISYSAYLWHQVLFALTRLYTFNTAHIDVYKILALLSFALAFISWKYV
                     EGPFRSASRVPLKPFIATLAVATLAVAGFGLAAVATDGFEARFNRSLDETQRLYHLNA
                     RESARLKKLGENHISHGECIIDSPSVSELFLSQFQHCARSRGKALLVIGDSHSGNLFR
                     ALASAAGRPDFLIRLGRGSCRPADQQAKCDIGEMADFLLTHKEQISAVVFAQSGFYLL
                     QGARRESILHNRTLLAPDQALIDRTLEFLIPISEAVPTYWFGPWIEPHLPLDNPRRMA
                     TTALDQLDFSALNRDMFAQLDRSIAQRASARAPLLNYRSQVREEGASADFIPLHVAGC
                     ITFRDSNHLSQCGERLMGQKMLDRIAR"
BASE COUNT         1867 a         3691 c         3420 g         1981 t
ORIGIN      
        1 cctcgttacg gcgatcctcg atggcgcggt ttttctcgaa ggcctgcgga tcgaacccac
       61 cgcactccac cgccagcgag tgatcgacga tcagctgcac cggcaccacc ggattcactt
      121 tcgccggatc accgccctgc tcggcaatgg cgtcacgcag gccggcgaga tcgaccagcg
      181 cggtctggcc gaggatgtca tggcacacga cgcgggccgg gaaccacgga aaatcgcgct
      241 cgcgtttcac ttcgatgatc tgtttcaggc actcatcaat gatggccgga tcggccttgc
      301 gcacgatgtt ctcggcgtgg atgcgggcgg tgtagggcaa ccggtcccag gcgccgggct
      361 ggatggcgtt gacggcttcg cgcgcgtcga agtagtcgag agaagtgccg ggcagggctt
      421 tgcggtattg ggtattcatc ggcagttgat ccacaacatg tttttgattc ttgacgagat
      481 gccagctcat tggtcatgcc tgaagctgtg gcaacggtcg gaaagaccat gccggttcaa
      541 tcgatccgtt gacccgtctc ggcactctgc attggccgaa gtttaccgca aaaagttgat
      601 catcgagtcg tgacgggcaa tgcgcagtcg tcttgaacaa ccctgtcgcc cctctcacgc
      661 acgtcatcga gccatgctcg gccttgcaac gcaaaaacgc accaggaaga tgcactgcca
      721 ctgcccgaag gcgcttttcc gtcctttgcc caagctgtta tgctctgcaa cgattgagct
      781 ccccccacga ctccaccagg gtgacagggc tgatgcactg agcatgacgc ccttactggc
      841 gcctcttgtg cggaaatgct tagccatcga actgtttggt gactgatatc tatctttttg
      901 tgcttcacgc agtgatgggt ctgttcttgt tccggcatca accgaacaac cggcatgaca
      961 gccgattgaa atggaatccg atccaatgat gctgggtctg gtccgcatcg ccctgcaacg
     1021 gccctacacc ttcatcgtgg tagcgctgct gctgctgatc atcggcccgc tggccgcgct
     1081 gcgcaccccc accgacattc tgccggcaat ccgcattccg gtgatcgcga cggcctggca
     1141 atacaccggg ttgccgccgg atcagatggc ggggcgcatg atcacacagt tccagcgcac
     1201 cctcagcacc accgtcaacg acatcgagca catggaggcc aactcctacg ccggctacgg
     1261 catcatcaag gtgttcttcc agccgggtgt cgatctggcg attgccaatg cccaggtgac
     1321 ggcagcctcg caggtgggga tccgacagat gccggccggc acgacaccgc cgctgatcct
     1381 gaactacgaa gcctcgacgg tgccaatcat ccagctggcg ctctcgggca agggaatgtc
     1441 cgagcaaagc ctggccgaca tcgggttgaa cagcgtccgc atcggtctga tcacggtgcc
     1501 gggtctggcg attccctacc cctatggcgg caagagccgg caggtgctga tcgatctcga
     1561 ccccaccgcg ctgcaggcgc gcggcctgtc ggcacaggat gtcgccaacg cactggcggc
     1621 acagaacctg atcgtgccga tcggcaagca gaagatcggt gaattcgaat acaccatgca
     1681 actgaacagc gcgcccgatg cgattgccga attgggcaat ctgccgatca gggtggtgaa
     1741 tggtgcgatg gtctacatcc gcgatgtcgc ccaggtacgc gatggcaatc cgccccagag
     1801 caacatcgtc catgtcgacg gcaaccgctc ggtgctgatg tcggtgctga agaacgggtc
     1861 gacctcgacg ctggcgatcg tcgatggggt caagcgcaag ctggccgaaa tcaccccctc
     1921 gctgccggag aacctgaagg tcgaactgat caacgaccag tcgctgttcg tgcgtgctgc
     1981 catcaccggc gtggcggtcg aaggcgtgat cgccgcggtg ctgaccagcc tgatgatcct
     2041 gctgtttctg ggcagctggc gggccacgct gatcatcgcg ctgtcgattc cgctgtcggt
     2101 gctgggggcg atcatcgccc tgtcgctgac cggagagacc ttcaacatca tgaccctggg
     2161 cggtctggcg ctggcgatcg gcattctggt cgacgatgcc accgtgacca tcgagaacat
     2221 caactggcac ctcgaacagg gcaagctggt cgaaaccgcg atcatggatg gcgcccggca
     2281 gatcgtcacg ccggccttcg tctcgctgct gtgcatctgc atcgtcttcg tgccgatgtt
     2341 ctttctcgaa ggggtcgccc gcttcctgtt cgtgccaatg gccaagtcgg tgatctttgc
     2401 gatgatctgc tcgttcctgc tgtcacgcac actggtgccg acgctggcca accatctgct
     2461 gcgcacccat gcgtcccaca ccgacctgca tggcaacgac ctggcgctgc cgccctcgcg
     2521 caacccgctg gtgcgcttcc agcgccgctt cgaggccggt ttcgagcggt ttcgtgacct
     2581 ctatcgacaa ctcctgctgc tggcattggc gaaccgtcgc cgtttcgtcg gcggtttcat
     2641 gctgctggtg ctggcctcct ttgccctggt gccctttctg ggccgcaact tcttcccggc
     2701 ggtcgacagc gggcagattc tgatccacgc ccgtgtgccg gtcggcaccc gggtcgaaga
     2761 gaccgctgcc cagttcgcca gaatccaggc ggccatccgc cgcatcattc cggccgatga
     2821 gatcgccacc ctggtcgaca acatcggcct gccgatcagc agcatcaacc tgacctataa
     2881 caacaccggg gtgatgggga gtcaggatgg cgacatccag atcgccctga agccgggaca
     2941 cgcccccacg gccgaccatg tgcgcgcgct gcgcgagcag ctgccacggc tgtttcccgg
     3001 cgtcaccttt tcgtttccgc ccgccgacat cgtcagccag attctcaact tcggtgcgcc
     3061 ggcaccgatc gatgtgcaga ttcgcggcaa caacctcgat gccaatttcg accatgccaa
     3121 cctgctgctg agcaagatcc ggcgcattcc cggcattgcc gatgcgcgca tccagcagtc
     3181 acgcagcaac ccggtcttca gggtcgatct cgatcgcacc caggcgcagc aggtggggct
     3241 caccacccgc gatgtgacca acagcctgct ggtcaacctg gccggcagca gtcaggtggc
     3301 acccaccttc tggctcaatc cggtcaatgg cgtctcctac ccgatcgtga tgcagacacc
     3361 gcaacatcag ctcgactccc tgtcgacact ggccaacctg ccgatcagcg gacctggcaa
     3421 cagcagcccg cagattctcg gggcgctggc cagcatcgaa cgcagccgca gcaatgcgct
     3481 ggtgagccaa tatgacatcc agaccatggt gcagattcat gcaaccaccc aagggcgcga
     3541 tctgggcgcg gtggccaagg aggtgcgacg cgtcatcgat gagaacgccc atgcgcgtcc
     3601 caagggcagc accgtcgtgc tgctgggtca ggtcaatacc atggagctct cctacagtgg
     3661 cctgctgttc ggcctgctgg gcgccgtggt gctgatctac ctgctgatcg tcatcaattt
     3721 tcagtcgtgg agcgacccct tcgtcatcat ctgtgcgctg ccggccgcgc tggccggcat
     3781 cgtctggatg ctgttcgtca cctacacccc gctgtcggtg ccggcgctga ccggcgccat
     3841 catgtgcatg ggggtcgcca ccgccaacag cgtgctggtg atcagcttcg cccgtgagcg
     3901 gctgctggaa ctgggcgatg cgacggcagc cgcactcgac gccggcttca tccgcttccg
     3961 tccggtgctg atgacggccc tggcgatgat catcggcatg gcgccgatgg cgctcggtct
     4021 gggcgaaggc ggtgagcaga atgccccgct cggccgggcg gtgatcggcg gtctgatctt
     4081 cgccaccctc gccacactga ttttcgtgcc tgtcgtgttc agcctcgtgc atgccggagc
     4141 ggggcatgac cgcatgcccg aggccgccac tggagaagcc catgcccgtt gacccttcca
     4201 ccccgacgct gtcgcgcacc cgcttgcgcc tcgccgcgct catgctgctg ctgttggctg
     4261 ccacgctggt ggtgatcggc attctcagcc gggccaacgc cgaagccgag ctgaaagccg
     4321 cgaccgaagc gcaggcaata cccacagtcg ccgtgctgct gccgataaag agcaaacagg
     4381 ccagcgagat cgagctgccc ggccggctcg aagcccacgc ccgggcgccg atccatgcgc
     4441 gggtggccgg ctatctgaag agctggaagg tcgacatcgg cagcgaggtc aaggccggtc
     4501 aactgctggc cgagatcgaa acccccgaac tcgaccagca gctgctgcag gcgcgggccg
     4561 accttgccag cgcacaagcc aatgccacca tggcgcaaaa gagcgcccgg cgctggcagg
     4621 cgatggtcgg ttccgacgcg gtgtcgcaac aggaggccga cgaaaagagc aacgacctga
     4681 tggtcaagca gtcggtggtc aaggcgctgc aggccaatgt cgaacggatg gaggtactga
     4741 agggcttcgc ccgcatccag gccccctttg ccggcacggt cactgcacgt tcgaccgacg
     4801 tcggcgcgct gatcaacccc ggcagcagtg gcggcgcgga gctgttcgtg attgccgaca
     4861 cccggcggct gcggctctat gtcagcgtgc cacagaatca gacagcgaag atcggaccgg
     4921 gcaccgaggc gcaggtgacg gtgcccgaac ggcccggcaa gcgctatccg gcgaaggtcg
     4981 aggcttcggc ccaggcggtg caggccgcct cgggcacggt gctgatgcag ctgagcgtgg
     5041 acaacagcgc cggtgaactg ctgcccggca gctacgcccg ggtgagcctg gcgctgccac
     5101 aggcggcgga agggctcaac atcccggtca gcgcgctgct gttcgacaag tccgggctgc
     5161 gcgtcgccac ggtggatgcc gagaaccggg tccggctgaa aacggtcacc ctgctgcgcg
     5221 atctcggcaa gagcatcgac atcggcacgg gattgacggc cagcgaccgg gtgatcgaaa
     5281 gcccgcccga tggcatcaat gagggcgatc cggtgcagat cgcagcgccc gagaacaaga
     5341 gccctgccga gggcaaacca gcgacggatg gcaagcgcga tgccggcaag ggctgagcgc
     5401 aggctcactg gcttgctggc gctgactctg gcgctggccg gctgttcgtt ggcaccgcca
     5461 ccgcaactgc cggaggtgcc ggtggccgcc agctaccggg aggaggcgcc ctggaccccg
     5521 gcccagcccg ccgaccagtt gccgcgtgac ggctggtggc aactctacgg cgatgccgaa
     5581 ctggatgccc tgcagcagcg gctgctggcg aacagccccg atctggccgc cgcgctggcg
     5641 cgctatcggc aagccgaggc ggtgacccgg cagagcgagt cggcgctgct gccgcgcctg
     5701 aacggttcgg ccacggtgca gcggctccag cagtcgcaga tgcgcccgtt gcgccctcct
     5761 ccggcgccga acgccgccga ccaatacaac tccaacaccc ttggcgtgtc cgccgactat
     5821 gaagtcgacc tgtggggccg catcagcaat caggtgacat cgagccagtc gacccagcag
     5881 gcggtggcca atgacctggc ctcggcacgc ctcagtctgg cggcacagct tgccgacggc
     5941 tacctggcgt tgcgaggact ggaccgggag agtgccttcc tgcagcagag cgtggagagt
     6001 tatgtcaagg cgctggaact gacccagact cgccatcagg gcggaatcgc ctccgggctc
     6061 gatgtcgccc gtgcccagac cctgctcgac aatgcacgtt cacaactggc gcagagccgg
     6121 gcgcagcggg cgctcgttga acatgcgatt gccgcactgg tcggcgagtc ggcctccagc
     6181 ttcagcatcg cgcccagact gaccgaggtc agggtaccac cggttccggt cgagctgccc
     6241 tcgaccctgc tgcagcggcg acccgacatc gccgccgcgc agcggcgggt cgcagcggcc
     6301 aatgccaatg tcggcgtggt gcgcgccggc tactatccgg cgatcatgct cggtgcctcg
     6361 gcgggctacc agagcagcga tgccagcgac tggatcaaag cccccaacct tttctggtcg
     6421 gtcggcccct ccctggtcgt caacctcttc gatgccggca agcgacgtgc cgaagaggca
     6481 cagtcacggg cgctgcttga cgaggccggc gcgaagtatc gcggcgtggt gctgacggcc
     6541 ttccagcagg tcgaggacaa tctggcgctg ctcaaccact accagagtgc gcgcgaggcc
     6601 gagcagtcgg ccgtcgctgc cgcccaacgt tcggtgcaac tggcgcagag ccgctaccgc
     6661 gaaggcgtgg ccaactacct cgaagtggtg accgcgcagg tcgcggccct gcagagcgaa
     6721 cgcaacgcgc tcaatctggc cgtgcgccag cagcgtgcca gcgtgcagct gattcgcgcg
     6781 ctgggcggcg gctggtcgga gagcgaagca ccctcgccgt gatgaatgcg gcgcctgcac
     6841 ccggctgcca ctgatctcca cggattgagt ccatcgagga tgaacgcctt gtcaaaccct
     6901 gccgtcgcag cgccccctga aacactgctc aaccggccat ttctgctggt gctgatggtc
     6961 gctgcggtct ttggcttctc attttcgagc tttttcctgc tgcccaaatt tctgtcggtc
     7021 gccctggcgg ccgacgcggt cagcatcggc agcatcacca cgctgttctg gctcgcttcg
     7081 gcattgacgg tgccctacat cggcggcttg atcgaccgcc acggccgcaa gcgctatgcc
     7141 ctgatcggtg cattgattct ggcactctcc tgcatcggct tcgtgtgggt cgacagtctc
     7201 ggtccgctga tctggctgct gcgcatcctg cagggcatcg gcttcacgct gtttctggtc
     7261 gcgctctcca cgctggcggt cgatctggcg ccaccggcgc gcctgggcca ggcgctgggg
     7321 atctttggcg cggtgatgat cctgaccaat gcggcaggac ccgccttcgc cgaatggacc
     7381 tcggcccact acggctggca gccggtgttc gtggccaccg ccgcggccgc gctgctggcc
     7441 gccctgctct gccgtctgct gccggagcag cggctgctgc tggccgacac ccgccatgcc
     7501 acgctgtggc aggtgctgcg ccgacccggc atcgggccga tcatcctggt gtcggccatg
     7561 gtgggctggg tgttcggcac gctcttcacc ttctaccagc cctgggcgct gtcgctcggc
     7621 atcgagcagg tggcgagcta cttcatcgcc tatgcggtgg tggcggtgac gatacggatc
     7681 ttcttcggcg acctgcccga ccggctcggg cggctgcggg tggtgcgcgc cagcctgctg
     7741 ctctatgtgc tgaccccgct ggcgctggtc tggctggacc agttgaacct gtatgtcgcg
     7801 ggcgccattc tcggactgac ccagggcgtc tactacccgg cactgaatgc agtggccatc
     7861 gactgcgcca ccccgcagga gcgcggcaag gtgatggcgg cctacagcgg cgccttcaac
     7921 ttcggtctgg cggcggcggg gttgatcctc ggccatctgg tgctggcgat gggctaccgc
     7981 agcgtcttcg ccttcgccag cgtgatcagt ctggtcgcct ttgcactgct ggccgtcacg
     8041 ccacagcgcc acttccggcc ggcaccgatg acgccggagt gaccctgccg gcgcgcaacg
     8101 ctgctttgcc aagctggaca gccctgctta tgctgcgacc tcccattcga tggatgcaca
     8161 acccatgaag gagcaactga tccatgactg aacagaaacc gggcgctccc tggtggcaga
     8221 ccctgcctgg ggttctgacc gccaccggca ccctgatcgt cgccgtgacc ggtctgctcg
     8281 gcatgctgca tcaaagcggc ctgctcggtg gcggcagcac ggccggcaag cagccggcca
     8341 ccgtcgaggc atccgctgct gcaccctcgg cggcccagcc caggccggcg gcaccggctg
     8401 acagcgacga agagaccgat cggctcgaca cagcggaaaa acgcccaaca accccgtcga
     8461 cagccaatgg cgaaccggcc gtcaccgcag gactgacggc cgcagcgcag caggcggagt
     8521 tccggcggcg ggtgcagcag ggtctctacc ccagcgaggt cgaaggccag tgcaaggagg
     8581 gggtcgagac cttccgcacc ctctggaagc cgctgccggc cggtgccgag ttcgtttcgg
     8641 tgcgcagcat gtccaaggag ggcttccaga ccaagcgcga caagttcgcc ggcgacggct
     8701 atgcgatcga gtcggtacac agctaccaga actgcaaggg tgtcgagcgc taccacgcca
     8761 cctggatcaa acgctgatcc agcctccacc ccgttgaaac acggcctcct gccaaccgac
     8821 tccgatgacg ccaaaccact tccgcttccc cacatcggtt gcacgccttt ttgaacagca
     8881 ccgtcacccg atgattcaat cgagcacgac caccgcatga gcacccggca ctaccgcccg
     8941 gagatcgatg gcctgcgggc gctggccgtc gtgccggtga tcctgtttca tgccggattg
     9001 cctgccttct ctggcggctt cgtcggcgtc gacatcttct tcgtgatcag cggttacctg
     9061 atcacctcga tcgtcatcaa ggagctcgac cagggccgct tcagcttcgg caacttctac
     9121 gaacgccggg cgcggcgcat cctgccggca ctgctgctgg tgatgctctg ctcgatcccg
     9181 ttctcctggc gctggatgat gccggatgaa ctgaaagact ttgcccagag cctgctggcg
     9241 gtgctgctgt tctcttccaa cctgctgttc tggcaggagt cgggctattt cgatggcgag
     9301 gccgacctca aaccgctgct gcacacctgg agcctggggg tcgaggagca gttctatctg
     9361 ctgttcccgc tgagcgcctg gctggcgtgg cgctgttttc gccggcatgt ctcgctgttc
     9421 tatgtcgcgc tgctgttggt gagcctgctg gcttcgcagt ggcgcctggc cggcgatgcc
     9481 agcgcggcgt tctacctgtt gccgtttcgt gcctgggagc tgctgctcgg cgcactgctg
     9541 gcgctgcacg aagccagagc gggcggacgc acagcgccac gttggagtga atggagcggt
     9601 gctgccgggt tggtgctgat tctgctcagc ctcgctcttt ttggaagaga gacgccgttt
     9661 ccgggcgtcg ccgccctgct gccgacgctc ggcaccctgc tggtgatcca tggcgcctcg
     9721 cccgccacct tggtcggcaa gctgctgggc cagcgactgc tggtcggcat cggtctgatc
     9781 agctacagcg cctacctgtg gcaccaggtg ctgttcgcgc tgacgcggct ctacaccttc
     9841 aacaccgccc acatcgatgt ttacaagata ttggcactgc tcagctttgc gctcgcgttc
     9901 atcagctgga agtatgtcga gggccccttc agaagtgcca gccgcgtgcc actcaagccc
     9961 ttcatcgcca cgctggccgt ggccaccctg gcggtggccg gctttggcct cgccgcggtg
    10021 gccaccgatg gcttcgaggc ccgcttcaac cgcagcctcg atgagacgca gcggctctac
    10081 catctcaatg ccagagagag cgccaggctg aaaaaactgg gagaaaacca catcagccac
    10141 ggcgagtgca tcatcgacag ccccagcgtc agcgaactgt tcctctccca gttccagcac
    10201 tgcgcccgca gccggggcaa ggcactgctg gtcatcggcg actcccactc cggcaatctc
    10261 tttcgcgcac tggcttcggc tgcggggcgg cccgattttc tcattcgcct cggtcggggc
    10321 tcctgtcgcc cggccgatca acaggccaag tgcgacatcg gcgagatggc cgacttcctg
    10381 ctgacacaca aggaacagat ctccgcagtg gtcttcgccc aatccggctt ctatctgctg
    10441 cagggtgccc gacgcgagtc gatcctgcac aatcggaccc tgctcgcgcc cgatcaggcc
    10501 ctcatcgacc ggacgctgga gtttctgatc ccgatcagcg aggccgtgcc gacctactgg
    10561 ttcggcccct ggatcgaacc gcatctgccg ctcgacaatc cacgccggat ggccacgacc
    10621 gcgctcgatc agctcgattt cagcgcgctg aaccgcgaca tgttcgccca gctcgaccga
    10681 tccatcgcgc agcgggcgag cgcccgggca cccctgctga actatcgctc gcaggtgcgg
    10741 gaagagggcg catccgctga cttcattcca ctgcatgtcg caggctgcat cacctttcgc
    10801 gacagcaacc acctcagcca gtgcggcgaa cggctgatgg ggcagaaaat gctggacagg
    10861 attgcccgct gacgcccgcc gcctttggat agaatcgctg gatcgccgac agcatcaaca
    10921 aaaagaagcc ggatgccggg tgaacacgga ccaaaccag
//
LOCUS       DAORCQ010000077        10842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4087_length_10842_cov_5.944099, whole genome shotgun sequence.
ACCESSION   DAORCQ010000077 DAORCQ010000000
VERSION     DAORCQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_10842_cov_5.944099"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /gene="radA"
                     /locus_tag="PKI23_08580"
     CDS             complement(<1..1225)
                     /gene="radA"
                     /locus_tag="PKI23_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNN37032.1"
                     /translation="MAKPTHRFFCADCGAEYSKWQGQCDGCRSWNRISEVAQSRLTGG
                     YAGAAGGTAELVTLDQISVSEAVRIGTGIGELDRVLGGGLVPGSAIVLGGHPGAGKST
                     LVLQALGNLARQHPVLYVTGEESLQQVAMRAQRLGVAGDQLKLLAETRVEAVIDCCLR
                     HRPRVLVIDSIQVMQLAELQSAPGSVGQVRESAARLTRFAKESDTILLLVGHVTKEGH
                     LAGPKVLEHMIDCSLMLEGSSDSRFRILRGQKNRFGAVNELGVFAMVEHGLREVKNPS
                     AIFLSGRREPAAGTLVTALWEGTRPLLVELQALVDQNHHGQPRRLAVGTDLNRLALLL
                     AILHRHGGLFLGDQDVFVNVVGGVKVDETSADLALVLALVSSFRNRPLPQDLLVFGEV
                     GLAGEIRPVPSGLERL"
     gene            complement(1238..2317)
                     /gene="alr"
                     /locus_tag="PKI23_08585"
     CDS             complement(1238..2317)
                     /gene="alr"
                     /locus_tag="PKI23_08585"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNN37033.1"
                     /translation="MARPIRADIDLAALRHNLQWIRQRHPGHELVAVVKANGYGHGLV
                     PTAQALAQADLLGVAAIDEAMLLRQAGLSQPILLMEGCFDASELDLAEQQGFELVIHS
                     HWQLELLEAMPPRQPLRLWIKIDSGMHRLGFAPGQVAGIQPRLRRLAGLRELVWMSHL
                     ACADEPEHPANARQLACFARAIAGLPGRCSVRASASILGDAALPMADHFLRPGLMLYG
                     ASPCADRSALALGLRPAMTFRSRLIALRELAVGEPVGYGGRFVTQRPSRIGVVAAGYG
                     DGYPRHASQGTPLLIRGQRVPLVGRVSMDMVTVDLTDLPAAAIGDEVILWGEGLPVDE
                     VAAAAGTIAYQLLCNVTGRVPYRYR"
     gene            complement(2323..3699)
                     /gene="dnaB"
                     /locus_tag="PKI23_08590"
     CDS             complement(2323..3699)
                     /gene="dnaB"
                     /locus_tag="PKI23_08590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNN37034.1"
                     /translation="MSADFETAALKLPPHSVEAEQSVLGGMMLDSTAWEQVAELLSAE
                     DFYLRDHRTIFEVMQRLIDSDQPIDVVTLSETLDRQGRLTEVGGLPYLVELARNTPTA
                     SNILAYATIVHERATRRRLIAVAGEIAASSFDPAGRSSAELIDEAESKVFQIADSRSS
                     GAGPTGVNALLSRALERIDELYNAKSPITGQSTGFHDLDEMTSGLQPADLVVVAGRPS
                     MGKTSFAMNMAEAAVITREQPVLIFSMEMPGESLMMRMLSSLGRIDQTRVRNGRLDDE
                     DWPRLTSAISLLKDKPLFIDDSPALSPAEVRARTRRVVREHGPVGLIVIDYLQLMRLG
                     GQVENRVNEISEISRSLKALAKEMNCPVIALSQLNRSLEQRTNKRPIMSDLRESGAIE
                     QDADLILFIYRDEVYHEDSKDKGRAEIIIAKQRNGPIGTVHLAFMGRYTRFDDLAPAS
                     YEESAYRS"
     gene            complement(3793..4239)
                     /gene="rplI"
                     /locus_tag="PKI23_08595"
     CDS             complement(3793..4239)
                     /gene="rplI"
                     /locus_tag="PKI23_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNN37035.1"
                     /translation="MEVILLERVGNLGNLGDRVAVKSGYARNFLIPFGKAAPATAKNI
                     AQLEAQRAELERAAQARQAAAEARAAALADLVVTLAVNAGDEGKLFGSIGTRDIAEAI
                     TAAGHPAEKSEVRLPNGTIRELGEHQVVLQLHNEVVAEIKLMVVRA"
     gene            complement(4305..5156)
                     /locus_tag="PKI23_08600"
     CDS             complement(4305..5156)
                     /locus_tag="PKI23_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37036.1"
                     /translation="MKALAEFAMRGRRQAALVAVVATALPMLFLLGAAIVGLLFLRRG
                     WSEGVTVLPWPLLVAGLWWWLGDPTAFIVMVMVVVQALLLRARVSWQPVWMAGIALAT
                     LVMLALNLWPLAGIEQLVQMTSQMMQSQLGEQWAGVEPQVTGWLNRVVVAMLAASDLL
                     FALLCLMLARHWQSVLYHPGGFRQEFHGFKVARPLALLLPLTMLAGTALWPPAALWLP
                     LLAVPFMLAAVGLVHGLVAARNLGKGWLIVFYLTLVVALPYMAPLLVLAAFLDVWFDF
                     RGRAAAT"
     gene            complement(5173..5400)
                     /gene="rpsR"
                     /locus_tag="PKI23_08605"
     CDS             complement(5173..5400)
                     /gene="rpsR"
                     /locus_tag="PKI23_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNN37037.1"
                     /translation="MTRYFRRRKFCRFSAEGVKEIDYKDIDTLLAYVSETGKIIPSRI
                     TGTKAKYQRQLSTAIKRARFLALIPYTDNRE"
     gene            complement(5466..5879)
                     /gene="rpsF"
                     /locus_tag="PKI23_08610"
     CDS             complement(5466..5879)
                     /gene="rpsF"
                     /locus_tag="PKI23_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNN37038.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIERYKRAIEEGGGAIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIEVGQPTLEELTTNFRYNDAVLRNLVIQRDEAVTEESPM
                     MRGEKENRERREREAEAEARQASREERGRANDEEE"
     gene            complement(6038..6907)
                     /locus_tag="PKI23_08615"
     CDS             complement(6038..6907)
                     /locus_tag="PKI23_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNN37039.1"
                     /translation="MPVTRYLWLSIAAAIATLLIKLGAWQLTDSIGYLSDGLESFVNL
                     AGALFALAMVVVARQPADHNHHYGHSKTEYFSVAFEGLLICIAAALIIWSALNRFLHP
                     QPLSNLDWGTLLSLLATAINFAVSRLLSQASRSHHSPALDADARHLMTDVWTTFGVIA
                     GVGLAGLTEAWWLDPLVAIAVAINILREGSKLMRGALDGLMDHALPPPMQARIEQILL
                     GYAAQGVRFGNLRTRAAGHLRFAQVDLFLPGSMTVAEAHDLADQVEQHVFSELGITLS
                     THVEPAVLDDRID"
     gene            complement(6920..7894)
                     /gene="corA"
                     /locus_tag="PKI23_08620"
     CDS             complement(6920..7894)
                     /gene="corA"
                     /locus_tag="PKI23_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNN37040.1"
                     /translation="MINAFELERGRLRQVQVESIDELRHCQPVWIDFVDASDQERDWA
                     STLYGIDLPEAHEIGDIEARARFFEEKTDNGDELHIRSDFFHEDDDGSQSIRVAFVLK
                     NSMLFSVHPEDLPQFRLVRMRARSQPGYVEDLQDVLLELYAADVEYSADVLEGIYERL
                     DGLSEQVLGHQIDDETAATVLSSIAKEENLNGHIRRNLMDTRRAISFLMRHRSLLSSE
                     QHNEARQILRDMDSIEGHTGFLFDKLTFLMDATIGFININQNKVVKIFSVASVCLLPP
                     TLIASIYGMNFELMPELKWSVGYPFSLLLMGISVVLPFWYFQRKGWLK"
     gene            complement(7891..9219)
                     /gene="yegQ"
                     /locus_tag="PKI23_08625"
     CDS             complement(7891..9219)
                     /gene="yegQ"
                     /locus_tag="PKI23_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNN37041.1"
                     /translation="MQITPELLAPAGTYRSMRYAFAYGADAVYAGQPRYSLRVRNNDF
                     TLESLATGIEEAHQQGRTFYVACNIAPHNAKVRRFIDDMVPVIEAGPDALIMSDPGLI
                     MLVRDRWPDMPVHLSVQSNVVNHAAVEFWRRQGVSRIILSRELSLEEIEEIQLASPQM
                     ELEVFVHGALCIAYSGRCLLSGYMNHRDPNQGACTNACRWEYDLLPAQQSSTGEVVST
                     CTPPAKVEPPPPADEEDGLFLLRERSRPDEVMPIFEDEHGTYILNSRDLRAVQHVERL
                     GRIGIRSLKIEGRTKSHFYAARTTQVYRRAIDDAAAGRPFDLNLMDVLEGLANRGYTE
                     GFYRRHPPGVYQNYEQGQSRSDRQQFVGEVVDSNDNWLTIEVKNQFAVGDALELMTPQ
                     GNHRFQLDHLEGRDGKAALIAPGSGHRVKIPRPPQCDAGEYALLIRNLVA"
     gene            complement(9290..10303)
                     /locus_tag="PKI23_08630"
     CDS             complement(9290..10303)
                     /locus_tag="PKI23_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNN37042.1"
                     /translation="MRTNQPILIAHRGASGYLPEHTLAAYALAALQGADFLEPDLVMS
                     RDGVLLARHDNQLDLTTDVADHPEFADRHCQRRVDGQLVSGWFSEDFTLAELKTLRAV
                     ERIPQLRPANRRFDRMFDIPTLAEVIALAKGLSQASGRPIGLYPETKHPSHFAALGLS
                     MEAPLVAALHGAGYRSAADPVFIQSFEVGNLRQLRGMTDLRLVQLLAESGQPFDVVTA
                     GGTLDYEQMATAAGLTAIAGYADGVGPQKSHFILAADAEGRLDPARASGFIDEAHAAG
                     LLVHPYTFRAENHFLPPALRGSSTEGLGDLVAELRLFLALGIDGFFCDQPDLGRRAID
                     LIE"
     gene            10726..10799
                     /locus_tag="PKI23_08635"
     tRNA            10726..10799
                     /locus_tag="PKI23_08635"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10758..10760,aa:Gly,seq:ccc)
BASE COUNT         2031 a         3607 c         3409 g         1795 t
ORIGIN      
        1 gcagccgttc gaggccactg ggcaccggac gaatctcccc ggccagcccc acttcgccga
       61 aaaccagcag atcctgcggc agcggccggt tgcgaaagct cgacaccagc gccagcacca
      121 gcgccagatc ggcgctggtc tcatcgacct tgacaccacc gacgacattg acgaagacat
      181 cctgatcacc gaggaacagc ccgccatggc ggtgcagaat cgccagcagc agcgccagac
      241 ggttcaggtc ggtgccgacc gccagtcggc gcggctggcc atggtggttc tggtcgacca
      301 gtgcctgcaa ttcgaccagc aagggccggg tgccctccca cagggcggtc accagggtgc
      361 cggcagccgg ttcgcggcgg ccggagagaa agatcgcgga ggggttcttc acctcgcgca
      421 gtccatgctc gaccatcgcg aagacgccca gctcgttgac ggcaccaaag cggttcttct
      481 gtccgcgcag aatgcgaaaa cggctgtcgc tcgaaccttc gagcatcagc gagcagtcga
      541 tcatgtgctc cagcaccttg ggcccggcca ggtggccctc cttggtaaca tggcccacca
      601 gcagcaggat ggtgtcactc tccttggcga agcgggtcag ccgcgcagcg ctctcgcgca
      661 cctgcccgac gctgcccggc gccgactgca actcggccag ctgcatcacc tggatggagt
      721 cgatcaccag cacccgcggc cggtgacgca ggcagcagtc gatcaccgcc tcgacccggg
      781 tctcggccag cagcttgagc tgatcgccgg ccacgccgag ccgctgcgcc cgcatcgcca
      841 cctgctgcag cgactcctcg ccggtgacat agagcactgg gtgctgtcgt gccagattgc
      901 ccaacgcctg cagcaccagc gtgctctttc cggcgccggg atggccaccc agcacgatgg
      961 ccgaaccggg caccagaccg ccacccagca cccggtcgag ttcgccgatg ccggtgccga
     1021 tccgcaccgc ttcgctcaca ctgatctgat ccagcgtcac cagttcggcc gtgccgccgg
     1081 cggcgccggc atagccaccg gtcagacggc tctgcgccac ttcactgatg cggttccagc
     1141 tgcggcagcc atcgcactgc ccctgccact tgctgtactc ggcgccgcag tcggcgcaaa
     1201 agaatctgtg ggtcggtttg gccatgctcc ctccctctca ccggtagcgg taaggcaccc
     1261 tgccggtgac attgcagagc agttgatagg cgatggtgcc ggcggcggcg gccacctcat
     1321 ccaccggcaa tccttcgccc cagagaatca cctcgtcacc aatcgccgcg gcgggcagat
     1381 cggtcagatc gaccgtgacc atgtccatcg acacccgacc caccagcggc acgcgctggc
     1441 cacggatcag cagcggggtg ccctggctgg catggcgcgg atagccatcg ccgtaaccgg
     1501 cggcgacgac gccgatccgg ctcggacgct gggtgacgaa ccgaccgcca tagcccaccg
     1561 gctcaccgac ggccagctcg cgcagggcga tcagccggct gcggaacgtc atcgccgggc
     1621 gcagacccag cgccagcgcc gatcggtcgg cacagggcga tgccccatag agcatcaggc
     1681 cgggccgcag aaagtggtcg gccatcggca gggcggcatc gccgagaatc gaggccgagg
     1741 cgcgcacact gcaccgcccc ggcaacccgg caatcgcccg ggcaaagcag gccagttggc
     1801 gggcattggc gggatgttcc ggctcatcgg cacaggcaag gtggctcatc cagaccagct
     1861 cccgcaggcc ggcaaggcgc cgcagccgcg gctggatccc ggccacctgc ccgggggcga
     1921 agccgagccg atgcatgccg ctgtcgatct tgatccacag ccgcagcggc tgccggggtg
     1981 gcatcgcctc cagcagttcg agctgccagt gagagtggat gaccagttca aaaccctgct
     2041 gctcggccag atccagctcg ctggcgtcaa agcagccctc catcagcagg atcggctgtg
     2101 aaagaccggc ctgccgcagc agcatggcct cgtcgatcgc cgccaccccc agcagatcgg
     2161 cctgcgccag cgcctgcgcg gtcggcacca gcccatgtcc atagccattg gccttgacca
     2221 ccgccaccag ttcatggccg ggatggcgct ggcggatcca ctgcaggttg tggcgcagcg
     2281 cggcgaggtc gatgtcggcg cggatcgggc gtgccatgcc ggtcaggacc tgtaggcgct
     2341 ctcttcatag ctggcgggcg ccagatcgtc gaaccgggtg tagcgcccca tgaaggccag
     2401 atggacggtg ccgatcgggc cgttacgctg cttggcgatg atgatctcgg cccgtccctt
     2461 gtccttggaa tcctcgtgat agacctcatc acggtagatg aacaggatca gatcggcatc
     2521 ctgctcgatg gcgccggatt cacgcaggtc ggacatgatc ggccgcttgt tggtgcgctg
     2581 ctccaggctg cggttgagct gcgaaagcgc gatgaccggg cagttcatct ccttggccag
     2641 cgccttcagc gagcgggaga tttcggagat ttcgttgacc cggttctcga cctgcccacc
     2701 cagccgcatc aattgcaggt agtcgatcac gatcagcccg accgggccat gctcgcgcac
     2761 cacgcggcgg gtacgggccc gcacctcggc cggcgacagc gccgggctgt cgtcgatgaa
     2821 gagcggcttg tctttcagca ggctgatcgc cgaggtgagg cgcggccagt cctcgtcgtc
     2881 gagccggccg ttgcgcaccc gggtctggtc gatgcgcccg agcgacgaga gcatgcgcat
     2941 catcagcgat tcgcccggca tctccatgct gaagatcagc accggctgct cgcgggtgat
     3001 caccgccgcc tcggccatgt tcatcgcaaa cgaggtcttg cccatcgatg gccggcccgc
     3061 caccaccacc aggtcggccg gttgcagccc cgaagtcatc tcgtcgaggt cgtgaaaacc
     3121 ggtgctctgg ccggtgatcg gcgacttggc gttgtaaagc tcgtcgatgc gctccagtgc
     3181 gcgcgacagc agcgcattga cgccggtggg cccggcaccg ctgctgcgcg aatcggcaat
     3241 ctggaacacc ttgctctcgg cttcgtcgat cagctcggcg ctgctgcgcc cagccggatc
     3301 gaagctgctg gcggcgatct cgccggccac cgcgatcagc cgtcgtcggg tggcgcgctc
     3361 atgcacgatg gtggcatagg ccaagatgtt cgaggcggtc ggggtgttgc gggccagctc
     3421 gaccagatag ggcaggccgc cgacttcagt gagacgaccc tgacgatcga gggtctcgga
     3481 gagggtgacg acatcgatcg gctggtcgct gtcgatcagc cgctgcatca cctcgaagat
     3541 ggtgcggtga tcgcggaggt aaaaatcctc ggcgctcaac agctcggcga cctgctccca
     3601 ggccgtgctg tcgagcatca tgccccccag caccgactgc tcggcctcga ccgagtgggg
     3661 cgggagcttc agcgccgcgg tttcgaaatc tgccgacatc gtctcaacct gcaatgaatc
     3721 ccgcaccatc gccgcgagac tgagcccgca ctgcttccgc ggcacacacc cgaatgtgtg
     3781 ccgcggaccg ggtcaggcgc gaaccaccat cagcttgatt tcggccacca cttcattgtg
     3841 cagctgaagc acgacctgat gctcgcccag ctcgcgaatg gtgccattcg gcagacgcac
     3901 ttcactcttc tcggccgggt gacccgccgc cgtgatcgcc tcggcaatgt cacgggtgcc
     3961 gatcgagccg aacagcttgc cttcgtcgcc ggcattgacc gccagggtga ccaccaggtc
     4021 cgccagcgcg gcggcccgtg cctcggccgc cgcctgccgt gcctgggctg cgcgctcaag
     4081 ctcggcccgc tgcgcttcga gctgggcaat gttcttggcg gtggccggag ccgccttgcc
     4141 gaacggaatc agaaagttgc gtgcataacc ggacttgacc gccacccggt cacccaagtt
     4201 gcccaggttg cccactctct caagcagaat cacttccatc gtattcacct cactacagga
     4261 gattgatcag ggtctgtcgc accgcggatg gctcgaaaag cctttcaggt cgcggcggca
     4321 cgcccacgaa aatcgaacca gacgtccaaa aaagccgcca acaccaacag cggcgccata
     4381 tagggcagag ccacgaccag cgtcagataa aagacgatca gccacccctt gcccagattc
     4441 cgtgccgcca ccaggccatg aaccagcccg acagccgcca gcatgaaggg cactgccagc
     4501 agcggcagcc agagggcggc cggcggccac agcgccgttc ccgccagcat ggtcaacggc
     4561 agcagcagcg ccaacggccg tgcgaccttg aaaccatgaa actcctggcg aaagccgccg
     4621 ggatggtaca gcacactctg ccaatgccgc gccaacatca gacagagcag cgcaaacagc
     4681 agatcggagg cagccagcat cgccaccacg acgcggttga gccagcccgt cacctgcggc
     4741 tccacgcccg cccactgctc accaagctga ctctgcatca tctggctggt catctgcacc
     4801 aactgttcga tgccggccag cggccagaga ttcagcgcca gcatcaccag cgtggccagc
     4861 gcgatgccgg ccatccagac cggctgccag gagacccgcg cccgcagcag cagcgcctgc
     4921 accaccacca tcaccatcac gatgaaggcg gtcgggtcgc ccagccacca ccagagcccg
     4981 gccaccaaca gcggccaggg cagcaccgtg accccttcgc tccagccacg gcgcaggaac
     5041 agcagcccaa cgatggcggc tcccagcagg aacagcatcg gcagggccgt ggccaccaca
     5101 gcgaccaggg ccgcctgccg gcgcccccgc atcgcgaatt cggcaagcgc cttcatggct
     5161 ggcgcgtgcc actcactcgc ggttgtcggt ataagggatc agcgccagaa agcgggcccg
     5221 cttgatggcg gtggagagct ggcgctgata cttggccttg gtgccggtga tccggctcgg
     5281 gatgatcttg ccggtttcag agacatacgc cagcagggtg tcgatgtcct tgtagtcgat
     5341 ctccttcacc ccttcggcgg agaagcgaca gaattttctg cgacgaaaat agcgggtcat
     5401 ggtctattcc tcaacaatgg ctacagaagg ccccgcaggg catcaccagc agagacggct
     5461 cgcctctact cctcttcgtc attcgcacga ccgcgctctt cgcggctggc ctggcgcgct
     5521 tcggcttcgg cctcgcgctc gcggcgctcg cggttctcct tttcgccgcg catcatcggc
     5581 gactcttcgg tgacggcctc gtcacgctgg atcaccaggt tgcgcagcac cgcatcgttg
     5641 tagcggaagt tggtggtgag ctcctcgagt gtcggctgtc ccacttcgat gttcatcaga
     5701 acatagtggg ccttgtggat cttgttgatc ggataggcca gttgacggcg accccaatcc
     5761 tcgagccggt ggatggcacc accgccctct tcgatggcgc gcttgtagcg ctcgatcatc
     5821 gcgggcacct gctcgctctg atcgggatga accaaaaaga caatctcgta gtgacgcatt
     5881 tctgctcctt atgggttgaa cagcctgcca cgcaccggat gatgcagcag tcaggcaagg
     5941 agcggcgggg ccgcggttgg aaaaaggcgg ctattctatg aaaacggctg accaattgaa
     6001 aggacgaatt ggaccgtgtt cgatgcaccg gccgatctca gtcgatccga tcgtccaaga
     6061 cagccggctc gacatgggtg gagagggtga tgcccagctc gctgaacaca tgctgctcca
     6121 cctggtcggc caggtcgtgc gcctcggcga ccgtcatcga tcccggcaga aacaggtcga
     6181 cctgggcaaa acgcagatgg ccggcggcac gggtgcgcag gttgccgaat ctgacgccct
     6241 gtgccgcata gccgagcaga atctgctcga tccgtgcctg catcggcggt ggcagggcat
     6301 ggtccatcaa tccatcgagg gcaccacgca tcagtttgct gccttcgcgc agaatgttga
     6361 tcgccacggc aatcgccacc agcggatcga gccaccaggc ctcggtcagc ccggccagcc
     6421 ccacgccggc gatgacaccg aaggtggtcc agacgtcggt catcagatgg cgtgcatcgg
     6481 catcgagcgc cggagagtgg tggctgcgcg aagcctgcga cagcagtcgc gacaccgcga
     6541 agttgatcgc ggtcgccagc agtgacagca gggtgcccca gtcgagattg ctcagcggct
     6601 gcggatgcag aaaacggttc agtgccgacc agatgatcag cgccgccgcg atacagatca
     6661 gcaacccctc gaaggcgacc gagaagtatt cggtcttgct gtggccatag tggtggttgt
     6721 ggtcggccgg ttgccgggcc accaccacca tcgccagcgc gaagagcgca ccggccaggt
     6781 tgacgaagga ctccagccca tcggagaggt agccgatcga gtcggtcagc tgccaggccc
     6841 ccagcttgat cagcagggtg gcgatggccg cggcgatgga gagccagagg tagcgcgtga
     6901 ccggcatggc cacatcctcc tatttgagcc agcccttgcg ctggaagtac cagaatggca
     6961 gcacgaccga gatgcccatc aacaacagcg agaaggggta acccaccgac cacttcagct
     7021 cgggcatcag ctcgaagttc atgccataga tgctggcgat cagcgtcggt ggcagcaggc
     7081 agaccgaggc gaccgagaag atcttcacca ccttgttctg gttgatgttg atgaagccga
     7141 tggtggcatc catcaaaaag gtcagcttgt cgaacagaaa gccggtgtgg ccttcgatcg
     7201 agtccatgtc gcgcagaatc tggcgtgcct cattgtgctg ctcgctcgac aacagcgacc
     7261 ggtggcgcat cagaaaggag atggcgcgcc gggtgtccat caagttgcgg cggatgtggc
     7321 cgttgaggtt ctcctccttg gcaatgctgg aaagcacggt ggcggcggtc tcgtcgtcga
     7381 tctgatgccc cagcacctgc tcgctcaggc cgtcgagccg ttcatagatg ccttcgagca
     7441 catcggcgga gtactccaca tcggccgcat agagttcgag cagcacatcc tgcagatcct
     7501 cgacatagcc cggctggctg cgggcacgca tccgcaccag ccggaactgc ggcagatcct
     7561 cgggatggac gctgaacagc atcgagttct tgagcacgaa ggcgacgcgg atgctctggc
     7621 tgccgtcgtc atcctcgtga aagaagtcgg agcggatgtg cagctcgtca ccgttgtcgg
     7681 tcttctcttc gaagaagcgg gcccgcgcct cgatgtcgcc gatctcatgg gcctcgggca
     7741 ggtcgatgcc atagagggtc gatgcccagt cgcgctcctg gtcggaagcg tcgacgaagt
     7801 cgatccagac cggctggcag tggcgcagct catcgatcga ctcgacctgc acctgacgca
     7861 ggcgaccacg ctccagctcg aaggcgttga tcatgcaacc agattccgga tcagcagcgc
     7921 atactcgccc gcatcgcact ggggcgggcg gggaatcttc acccgatgcc cggagccggg
     7981 tgcgatgagg gcggccttgc catcgcgccc ttccagatgg tcgagctgaa accggtggtt
     8041 gccctgcggc gtcatcagct ccagcgcatc gccgacggca aactggttct tcacctcgat
     8101 ggtgagccaa ttgtcatttg aatcaaccac ttcaccgacg aactgctggc gatcactgcg
     8161 cgactgtccc tgctcgtagt tctggtacac ccccggtgga tggcggcgat agaagccctc
     8221 ggtgtagcca cggttggcca acccttcgag cacatccatc agattcaggt caaacggccg
     8281 gccggccgcc gcatcgtcga tggcgcggcg gtagacctgg gtggtgcggg cggcatagaa
     8341 gtggctcttg gtgcgcccct cgatcttcag cgaacggatg ccgatgcggc cgagccgctc
     8401 gacatgctgt acggcccgca ggtcgcgtga gttgagaatg taggtgccgt gctcatcctc
     8461 gaagatcggc atcacctcgt cagggcggct gcgctcacgc agcaggaaca gcccatcctc
     8521 ctcgtcggca ggtggcgggg gctcgacctt cgctggcggg gtgcaggtcg acaccacttc
     8581 gccagtgctg ctctgctggg ccggcagcag gtcatactcc cagcgacagg cgttggtgca
     8641 ggcgccctga ttggggtcgc ggtggttcat gtagcccgag agcagacagc gacccgaata
     8701 ggcgatgcag agcgcgccat ggacgaagac ctcgagctcc atctgtggcg aggcgagctg
     8761 gatctcctcg atctcctcca gcgacagctc gcgcgacaga atgatgcggc tcaccccctg
     8821 ccggcgccag aactcgaccg cggcgtggtt gaccacattc gactgcaccg acagatgcac
     8881 cggcatgtcg ggccagcggt cgcgcaccag catgatcagg cccggatcgg acatgatcag
     8941 cgcatccggc ccggcctcga tcaccggcac catgtcatcg atgaagcgcc ggaccttggc
     9001 attgtgcggc gcgatgttgc aggccacgta aaaggtccgc ccctgctggt gggcctcctc
     9061 gatgccggtg gccagcgact ccagcgtgaa gtcgttgttg cgcaccctga ggctgtagcg
     9121 cggctggccg gcatagaccg catcggcacc ataggcaaag gcgtagcgca tgctgcggta
     9181 ggtgccggca ggcgccaaca gttcgggggt gatctgcatg gtgttgttcg cacttgggct
     9241 gcggtttgag cggggcgcaa tggtatcacg gcactccacc gcgttgatct cattcgatca
     9301 gatcgattgc ccgacgcccg agatcgggtt ggtcgcagaa gaaaccatcg atacccaatg
     9361 ccagaaacag gcgcaactcc gccaccaaat cgcccagccc ctcagtggaa ctgccccgca
     9421 gtgccggcgg cagaaagtgg ttctcggcac gaaaggtgta gggatgcacc agcagaccgg
     9481 ccgcatgggc ctcgtcgatg aagccgctgg ctcgggccgg gtcgagccga ccctcggcat
     9541 cggccgccag aatgaaatgg ctcttctgcg gaccgacgcc atcggcataa ccggcaatgg
     9601 cggtcagtcc ggccgccgtg gccatctgct cgtagtcgag cgtgccgccg gcagtcacca
     9661 catcgaaggg ctgaccgctc tccgccagca actgcaccag tctgagatcg gtcatgccgc
     9721 gcaactgccg caggttgcca acctcgaacg attgaatgaa caccggatcg gccgcgctgc
     9781 ggtagccagc accatgcagc gcggccacca ggggcgcctc catcgacaac cccagtgccg
     9841 caaagtggct gggatgcttg gtttccggat agagcccgat cggacggcca ctggcctggg
     9901 agagcccctt ggccagcgcg atcacctcag cgagggtggg aatgtcgaac atccgatcga
     9961 aacggcggtt ggccggacgc agctgcggga tccgctcgac cgcgcgcaat gtcttcagtt
    10021 cggcgagggt gaaatcctcg ctgaaccagc cactcaccag ctggccatca acccggcgct
    10081 gacaatgccg gtcggcaaac tcgggatggt cggcgacatc ggtggtgaga tcgagctgat
    10141 tgtcgtgacg cgccagcagc accccgtcac ggctcatcac cagatcgggt tcgagaaaat
    10201 cggccccttg caaggccgcc agcgcatagg cggcaagggt gtgttcaggc agatagccgc
    10261 tggcgccgcg gtgggcgatc aggatgggct gattggttcg catgggcatc cccgattcga
    10321 gagaatgcca tggtaaaagt cggtggccat cgcgcaaagc gcccggcacg attgtacgtg
    10381 ccggtcagca cccgccgcgc atggccactc gatgcgtcaa agctgttgca gcaaattgac
    10441 agcaaacagc gccccgacaa acagcagata gctggccagc aatccaaggc gcatccgcat
    10501 cggccgcggc agtggcggaa cgacactctg gcactgctgc gtgccctgct gcgagcaaag
    10561 tgatcgaatc atgatgaacg ctcccatgtg ttcagtgaaa acataggcag aataggcgcc
    10621 gctgtgttca ctgtcaacat ttttctttgc gggcgcgacc ctccgcagca gcacccggcc
    10681 aacaccttgc gtggcgcccg ggacaacagt atcgttccgg ctgccgcggg tgtggttcaa
    10741 tggcagaacg gcggcttccc aagcctcaag cgtgggttcg attcccatca cccgctccac
    10801 tgcatcattc tgcggacttc cgcagaagtc caagagagtc tg
//
LOCUS       DAORCQ010000078        10679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4182_length_10679_cov_5.782568, whole genome shotgun sequence.
ACCESSION   DAORCQ010000078 DAORCQ010000000
VERSION     DAORCQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4182_length_10679_cov_5.782568"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..238
                     /locus_tag="PKI23_08640"
     tRNA            163..238
                     /locus_tag="PKI23_08640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:196..198,aa:Gly,seq:gcc)
     gene            complement(299..616)
                     /locus_tag="PKI23_08645"
     CDS             complement(299..616)
                     /locus_tag="PKI23_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNN37043.1"
                     /translation="MLPDITTLSDDELKELSKKIQALISKREEQRKDEVAAQIKALAD
                     SAGLTVEIGGAEKSKRKGRSKLQPKFRHPSDPSLTWSGIGAKPKWLREMEKNGRRTEE
                     FRI"
     gene            836..1774
                     /locus_tag="PKI23_08650"
     CDS             836..1774
                     /locus_tag="PKI23_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN37044.1"
                     /translation="MVAALDIRNLTKSYATGKLALRGVDLRVEQGDFFALLGPNGAGK
                     STLIGTVSGLVNKSGGTVEIFGHDIDREFSLAKKQLGVVPQEFNFNQFEKVIDIVMTQ
                     AGLYGLPVTLARARAEKYLGKLGLWEKRNERSRFLSGGMKRRLMIARALVHEPRMLIL
                     DEPTAGVDIELRRSMWDFLKEINRDGTTIILTTHYLEEAESLCRNIAIIDQGSIVANT
                     DMKSLLQQLNTETFVLDLRQNLDQAPQLTDYRLRLIDAHTLEVDLLKAQSLNDCFGQL
                     SALGIEVISMRNKSNRLEELFLNMVESSQNKNGAVA"
     gene            1771..2544
                     /locus_tag="PKI23_08655"
     CDS             1771..2544
                     /locus_tag="PKI23_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN37045.1"
                     /translation="MNWLEQWRALLTIVVREVRRFTRIWMQTLLPPAISTGLYFVIFG
                     ALIGARIGKMGGYDYMEFIVPGLVMMAVITNAYSNVVSSFYNTKFQRSIEEMLVSPTP
                     NYIILLGYVLGGTARGLMVGAIVIALSLFFTHLQVHHMLVTLMVVVMTAVLFSLGGFI
                     NAVFAESFDDISIVPTFVLTPLTYLGGVFYSIDLLPEFWRQVSLLNPILYMVNSFRYG
                     ILGVSDVSIGWALGMIFSCIVVLALYALSLLNRGVGIRS"
     gene            complement(2554..5964)
                     /locus_tag="PKI23_08660"
     CDS             complement(2554..5964)
                     /locus_tag="PKI23_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNN37046.1"
                     /translation="MAEVSTLKLPIDSPARDAALDTTRSLIVQAPAGSGKTELLIRRM
                     LRLLAQVSRPEEILAITFTRKAAAEMQARILAALHGAQSAPPAEPHRLENWHLARQAL
                     AQDQRHGWRLLENPNRLNICTIDSFNGRLVRRLPLAAGLAFNAHLADQSEPAYHDAAR
                     ELLDSLNDAVPWADALEQLLLHLDNDFDRAERLFVELLKIRDQWLPHLYLEEGEQLRH
                     HLERCLTELIDERLADAARTLRNLPDTLWPLVRFAVDQLHLAQIDSPILPLLSSPLPP
                     PAEHSALPAWQALTQLLLTGEGEWRKRIDRNQGFPPASDARDPQQKSLLSERKAQMGQ
                     LLASLADDGASREALLEIRGLPRHGYSDQHWALLQALIALLKPLAALLKLVFQRRNEI
                     DHNEVAAAALRALGGDEQPTDLALALDSQIRHLLVDEFQDTSSTQIALLSRLTHGWEA
                     GDGRTLFLVGDPMQSIYRFREADVSHFLAAFHGNFNQLPLDAVRLTTNFRSTRQVVEW
                     INDAIGNAFPSDESLAIGAVPYSRATAFDGSTATPDAITATLLSGPGARLLEAKALVT
                     CLQQALQQPEHTIAVLVRARTHLQPLISELKSAGIAFSAVEVEPLERRIAIQDLRTLT
                     AALLAPADRLAWFALLRSPLVGLTLADLTLIADQGQDPVIELLQQAKRCAQLSSDGAQ
                     RCQRLLTRITPSLQQARRRPLRDWIEGCWLLLGGPATLRSAQAHDEAEAYFQLLERIE
                     TPGLPLDLDQLDAELSRLHAPSQGQTRVQLLTIHKAKGLEFDVVLLPGLEQSPPPAKE
                     KLLNWHVNRGPHAPRLLMAPIAPGADRAQSNPINGYLKEIERKREAQEAIRLLYVAAT
                     RARARLHLFATVKRDPSGQPRAPDRRSFLAMLWPGILPEARWIEAVDEIDGEAKSAAQ
                     WHRLPADFAAPHLPDDGLLSRFRGHDFPYDPQRLRFKLQLDELDTQGARQFGELLHRA
                     IETVAQEGIAQWDSQRLARSRDGWLAMLRQLGYRGAAVPAVTRLQQIVQDCLQSPTFR
                     WLLDPLHQQAENELAVSGWIDGELRHCVIDRTFVNHSGIRWIIDYKSSRPTADEALDA
                     FLARETGQYRQQLLDYRTLMGHIDARPIRTALYFAQIDLLHEIGHEAR"
     gene            complement(5957..8614)
                     /locus_tag="PKI23_08665"
     CDS             complement(5957..8614)
                     /locus_tag="PKI23_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNN37047.1"
                     /translation="MSQPDATLAALDHHSLLLTPNLRLSRTLLNRHAQLQRERGVKAW
                     RSPAILPFSGWLQQLYLQQQALSSDRRWQATLLDEWAQRLLWQQAVESALPDQGLFRA
                     ADLAVQASDAWELLQLWQVSESRLINELAVDESFGWLAQWVAHFRGRCREQGWISSAE
                     LPALITTAIDHGQISLPRQLLLYAFDEWPPAWQRLLDRANAAGATLRQLDADPRPSQP
                     QRLPCDQERDELQAIAAWCRRVLIERGERVRIGVVAPELPRVRGQLLHALREQLEPHA
                     LLPSTPPYTPPVNLSAPLPLPAVPIIADALTLLRLTTGTDLSATALRQILRSPFIGEE
                     RRQAQFEAALQQQRRHEWSPPLLRAALLQLPNSHASPFSSTLIDLLDQAQQGSAAQPP
                     SYWARRFSSALLALGWPGERTLDSEELQAVETWREALQRFAMLDPYTAPLAAGTALQR
                     LTELLALASFQPQSGDSPIQILGLLEASGLQFDQLWIMGLDRGNWPTAPAPNPLLPIG
                     LQRRLDLPRATAEREIEFAARITRRLLGAADQVILSHACRKDEEPLEPSPLISGWPTT
                     TAAALGLVTSAIEPLRQQPLATLPTEDHQGLPLVLTDDRPSRGGTQLLKDQAACPFRA
                     YANHRLRLLPAEPPQLGFDARERGTFSHLLMERFWSEVRSQERLQHADAAQRDAWCQQ
                     AVDAVMQQHHRTAGAGRLWQIERQRLLRLLQTALLLDLQRPPFTVTVEQPAALSIGPL
                     RLRTRADRIDRLEDGSLLLIDYKGSEHNVTEWLGERPDEPQLPLYLLQQDDRQPIAAI
                     AFFMLDTERSKLAGLGAHDDLAPGIKLPRHDEMATDPWNTQIDLWQQHLTRLAEEFAA
                     GIARVTPKRLPHSCEYCPHRPLCRIDDHG"
     assembly_gap    8650..8749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8791..9024)
                     /locus_tag="PKI23_08670"
     CDS             complement(8791..9024)
                     /locus_tag="PKI23_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HNN37048.1"
                     /translation="MNESKKEPSKQPESLSILQTIGSVLAAALGVQKRANLERDFAHG
                     KASRFIIAGVIFTVLFVLTVYGVVQLVLSQVAR"
     gene            9197..10342
                     /locus_tag="PKI23_08675"
     CDS             9197..10342
                     /locus_tag="PKI23_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNN37049.1"
                     /translation="METLRPFSIEIPEAQLIDLRQRLAATRWPEAETVDDWSQGVPLA
                     YLREFCSYWAQEYDWRVTERRLNVFPQFKSQIDGLDIHFLHVRSRHAQALPLLITHGW
                     PGSIVEFHKVIAPLSDPTAFGGSADDAFHVICPSLPGYAFSDKPRQTGWGVEKIATVW
                     DALMRRLGYQRYVAQGGDWGAAVTTAIGTQNLGACIGIHLNMPTASPTPQALSSPTER
                     DQRALAAAAHYQQWEGGYSKQQATRPQTLGYGLVDSPVGQAAWILEKFRAWTDCQGHP
                     ENILSRDELLDNIMLYWLTASAASSARLYWESFGSFGGDRSAGVELPTGCSIFPKEIV
                     PTPRSWAEQRYRNIVYWNELDRGGHFAAFEQPQRFVEELRACFALMR"
     gene            10352..>10679
                     /locus_tag="PKI23_08680"
     CDS             10352..>10679
                     /locus_tag="PKI23_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,3-beta-glucosidase"
                     /protein_id="HNN37050.1"
                     /translation="MALEKKIRGVNLGSWLLLEKWMVPSLFAGLEATDETSWCVELGD
                     RAAEPLHRHWNSFITRDDFVWLAARGINAVRIPVGHWIFGPDYPYHRSYGAHRHPFVT
                     GGIELLD"
BASE COUNT         1789 a         3132 c         3548 g         2110 t
ORIGIN      
        1 cccttcgtct agtggcctag gacaccgccc tttcacggcg gtaacagggg ttcgagtccc
       61 ctaggggacg ccaacccgat ggggcttgca gtgggtcgcg aaaattgtag aatgtgcgtc
      121 cgtttgcggt gagtcgaaga tcacgcgggc gaaggtcaaa aagcgggaat agctcagttg
      181 gtagagcgca accttgccaa ggttgaggtc gcgagttcga gcctcgtttc ccgctccaat
      241 caaaaaggcg ccatggtgat gaagaccatg gcgccttttt cggttcgggt ccgctggatc
      301 agatgcggaa ctcttcggtg cgtcgaccgt ttttctccat ctcgcgcagc catttcggtt
      361 ttgcgccgat gccggaccag gtcagacttg ggtcggatgg gtggcggaat ttcggttgca
      421 acttgctgcg gcctttgcgt ttggatttct ccgcaccgcc gatctccacg gtcagaccgg
      481 cggagtcggc cagcgccttg atctgtgcgg ccacctcatc cttgcgctgc tcctcgcgct
      541 tgctgatcag ggcctgaatc ttttttgaaa gctctttgag ttcgtcgtca ctcagggtgg
      601 taatatctgg aagcatcggt ttctcctgtg aatgaatttc agcaagtata acgatcaaat
      661 cgctggggtt gaatatttaa tttcaattgc gcctgttctg gtctggctct cttgctgtcg
      721 cttttgtggg gcaggtgatc ctgaatcctt acatgaaatc attgctgcag ggcagccgtt
      781 accggcatgg tgcttaaagg ttttcttttt ctggtcgagg tgaaaggcag gtcttatggt
      841 tgcggcattg gatatcagga atttgaccaa gagctatgcc acgggaaagt tggccttgcg
      901 tggtgtcgat ctgcgggtcg aacaggggga tttctttgcc ctgctcggtc ccaatggagc
      961 tggcaagtcg accctgatcg gcacggtttc gggtctcgtc aataaaagtg gcggcaccgt
     1021 cgagattttt ggccatgaca tcgatcggga attttcgctg gccaagaagc agttgggtgt
     1081 cgtgccgcag gagttcaatt tcaatcagtt cgaaaaggtg atcgacattg tcatgacgca
     1141 ggcagggctc tatggattgc cagtgacact ggcgcgggca cgggccgaga agtatctcgg
     1201 caagctgggg ctgtgggaga agcgcaatga acgctcccgt tttctttcgg gcggcatgaa
     1261 gcggcgattg atgatcgccc gggcgctggt gcatgaacca cgcatgctga ttctcgatga
     1321 gccgactgcc ggggtggaca tcgagctgcg ccgttcgatg tgggattttc tcaaggagat
     1381 caacagagat ggcaccacga tcatcctgac gacccactat ctggaagagg ctgaaagcct
     1441 gtgccgcaac attgcgatca tcgaccaggg cagcatcgtt gccaacaccg acatgaagag
     1501 cctgttgcag cagttgaaca ctgaaacctt cgtgctcgat ctgcggcaga atctcgatca
     1561 ggcaccgcaa ctgaccgact accggctgcg gctgatcgat gcgcacacgc tggaggtcga
     1621 tctgctcaag gcgcagagtc tgaatgactg ctttggccaa ttgtcggcat tgggcatcga
     1681 ggtgatcagc atgcgcaaca agagcaaccg tctcgaggag ctgttcctca acatggtcga
     1741 gagcagtcag aataaaaatg gagcggtggc atgaactggc tggaacagtg gcgcgccttg
     1801 ttgacgattg tcgtgcggga ggtgcggcgt ttcacgcgta tctggatgca gaccctgctg
     1861 ccgccggcga tcagcaccgg gctctatttc gtgatcttcg gggcgctgat cggggcacgg
     1921 atcggcaaga tgggcggtta tgactacatg gaattcatcg ttccggggct ggtgatgatg
     1981 gccgtgatca ccaatgccta ttccaatgtg gtgtcgtcgt tctacaacac caaattccag
     2041 cgctcgatcg aggagatgct ggtctcgcca acgccgaact acatcatcct gctcggctat
     2101 gtactgggcg gaaccgcccg tgggctgatg gtgggtgcga tcgtgattgc gctgtcgctc
     2161 tttttcaccc atctccaggt ccatcatatg ctggtgacgc tgatggtggt ggtgatgacg
     2221 gcagtgctgt tttccctcgg cggtttcatc aatgcggtgt ttgccgagag ttttgatgac
     2281 atctcgattg tgccgacctt cgtgttgacg ccactgacct atctgggtgg ggtgttctat
     2341 tcgatcgatc tgctgcctga gttctggcgg caggtatcgt tgctgaatcc gattctctac
     2401 atggtcaaca gctttcgtta tggcattctt ggcgtttcgg atgtttcgat cggttgggca
     2461 ctcggcatga tcttctcctg catcgtcgtg ctggcgcttt atgcgttgag cctgctcaat
     2521 cggggtgtcg gcattcgctc ctgatctgct tcttcagcgc gcttcgtgcc cgatctcgtg
     2581 cagcaggtcg atctgcgcga agtaaagcgc ggtacggatc ggtcgggcat cgatgtggcc
     2641 catcagggtg cggtaatcga gcagttgctg ccggtactgc ccggtttcac gcgccagaaa
     2701 cgcatccagt gcctcgtccg ctgtgggtct gctgctttta tagtcgatga tccagcgtat
     2761 accgctgtga ttgacgaagg tgcgatcgat cacgcagtgg cgcagttcgc catcgatcca
     2821 gccgctgact gcgagctcgt tctcggcctg ctgatgcagc ggatcgagca gccagcgaaa
     2881 ggtgggtgac tgcaggcaat cctgcacgat ctgctgcagc cgtgtcaccg cgggcacggc
     2941 tgcgccccgg tagccgagct gccgcagcat cgccagccag ccatcgcggc tgcgggcgag
     3001 gcgttgggaa tcccactgtg caatgccctc ctgcgcaacg gtttcgatcg cgcggtgcag
     3061 cagctcgccg aactggcggg cgccctgcgt gtcgagctcg tccagttgga gcttgaaccg
     3121 cagccgttgc gggtcgtagg gaaagtcatg accacgaaag cggctcaaca ggccgtcatc
     3181 gggcagatgc ggcgcggcga agtcggccgg cagtcgatgc cactgcgcgg cgcttttggc
     3241 ttcgccgtcg atctcgtcga ccgcttcgat ccagcgtgct tcgggcagga ttccgggcca
     3301 gagcatggcc agaaagctgc gtctgtcggg tgcgcgtggc tggccgctcg ggtcgcgctt
     3361 gacggtggcg aacagatgca ggcgggcgcg ggcgcgggtg gcggcgacat agagcaggcg
     3421 aatcgcctcc tgcgcctcgc gcttgcgctc gatctccttc aggtagccgt tgatcgggtt
     3481 cgattgcgcc ctgtccgcgc caggggcaat cggcgccatc agcaatctgg gggcgtgcgg
     3541 gccgcggttg acatgccagt tgagcagttt ctccttggcc ggaggcggac tctgttccag
     3601 accgggcagc agcacgacat cgaactcgag acccttggcc ttgtggatgg tcagcagctg
     3661 cacccgggtc tgaccctggc ttggggcatg gaggcgggag agttcggcat cgagctggtc
     3721 gagatcgagc ggcagacccg gggtttcgat tcgctccagc aactgaaaat aggcttcggc
     3781 ctcgtcatgg gcctgtgccg atcgcagcgt cgctggacca ccgagcagca gccagcagcc
     3841 ttcgatccag tcccgcagtg gccggcgtcg cgcctgttgc aggctggggg tgatccgtgt
     3901 cagcagccgc tgacagcgct gcgcaccgtc gctggagagt tgtgcgcagc gcttcgcctg
     3961 ctgcaacagt tcgatcaccg ggtcttgccc ctggtctgcg atcagggtca ggtcggcgag
     4021 cgtgagcccc accagtgggg agcgcagcag tgcgaaccag gccagccggt cggccggagc
     4081 gagcagggca gcggtcaaag tgcgcagatc ctgaatggcg atgcggcgct ccagcggctc
     4141 gacctcgacc gccgaaaagg cgatgccggc cgatttcagc tcgctgatca gcggctgcag
     4201 atgggttctg gcgcgcacca gcacggcaat ggtgtgctct ggctgttgca gcgcctgttg
     4261 caggcaggtc accagcgcct tggcttcgag cagccgtgcg cctgggccgc tgagcagggt
     4321 ggcggtgatg gcatccggtg tcgccgtgct gccgtcgaag gccgtggccc gactgtaggg
     4381 caccgcaccg atcgccagcg attcatcgct gggaaaggcg ttgccgatcg catcgttgat
     4441 ccactccacc acctggcggg tggagcggaa attggtggtc aggcgcacgg catcgagcgg
     4501 caactggttg aaatttccgt ggaatgcagc gaggaagtgg ctgacgtcgg cttcgcgaaa
     4561 gcggtagatc gactgcatgg gatcgccgac caggaacagc gtgcggccgt cgcccgcttc
     4621 ccagccatgg gtgagccggc tgagcagcgc gatctgtgtc gaggaggtgt cctggaactc
     4681 gtcgaccagc aggtggcgaa tctgactgtc gagcgccagc gccagatcgg tgggctgttc
     4741 gtcgccgcca agtgcccgca gtgccgccgc agcgacttcg ttgtgatcga tctcgttgcg
     4801 ccgctggaat accagtttga gcagggcggc gagcggtttg agcagcgcga tcaacgcttg
     4861 cagcagcgcc cagtgctggt cgctgtagcc gtgcctcggc agcccccgaa tctccagcag
     4921 tgcctcgcgg ctggcgccat cgtcggcgag tgaggcaagc agttggccca tctgcgcctt
     4981 gcgctcgctc agcaggcttt tctgctgtgg gtcgcgtgca tcgctggcgg gtggaaagcc
     5041 ctggttcctg tcgatgcgct tgcgccactc gccttcgccg gtcagcagca gttgggtcag
     5101 cgcctgccag gcgggcaggg cgctgtgctc ggctggtggc ggcagcggac tggacagcag
     5161 cggcaggatc ggcgaatcga tctgggccag atgcagctga tcgaccgcga aacggaccag
     5221 cggccagagt gtgtcgggca ggttgcgcag cgtgcgtgcg gcatcggcga gccgttcgtc
     5281 gatcagttcg gtcagacagc gctcgaggtg gtggcgaagc tgctcgccct cctccagata
     5341 gaggtggggc agccactggt cgcgaatctt cagcagctcg acgaacagcc gttcggcgcg
     5401 gtcgaagtca ttgtccagat gcagcagcaa ctgctccagt gcatcggccc aggggactgc
     5461 atcgttcagg ctgtcgagca gctcccgggc ggcgtcgtgg taggctggtt cgctttgatc
     5521 ggcgaggtgg gcattgaagg cgagcccggc cgccagcggc agccgccgca ccagtcggcc
     5581 attgaagctg tcgatggtgc agatgttcag ccggttggga ttttcgagca gccgccagcc
     5641 atggcgttga tcctgcgcca gtgcctgccg cgccagatgc cagttctcta gccgatgggg
     5701 ctcggcgggt ggcgcactct gcgcgccatg cagcgctgcc aggatgcgcg cctgcatctc
     5761 tgccgccgcc ttgcgggtga aggtgatggc cagaatctcc tcggggcggg agacctgggc
     5821 cagcagtcgc aacatgcggc gaatcagcag ctcggtcttg ccggagccgg ccggcgcctg
     5881 cacgatcagc gagcgcgtgg tgtcgagtgc cgcgtcacgg gccgggctgt cgatgggcag
     5941 cttcagggtg ctgacttcag ccatggtcat cgatccggca gagggggcgg tgcggacaat
     6001 attcgcaact gtgcggcagg cgcttgggtg tcacgcgggc gatgccggcg gcaaactcct
     6061 cggcgagccg ggtcagatgc tgctgccaga ggtcaatctg cgtgttccag gggtctgtcg
     6121 ccatttcgtc gtgccggggc agcttgatgc cgggggccag atcgtcgtgc gcaccgagtc
     6181 cggccagctt gctgcgttcg gtgtcgagca tgaagaaagc gatggcggcg atcggctggc
     6241 ggtcgtcctg ttgcagcagg tagagcggca attgtggctc atctggacgc tcgcccagcc
     6301 attcggtcac attgtgttcg ctgcccttgt agtcgatcag cagcaggctg ccatcctcga
     6361 gccgatcgat gcggtcggcg cgggtgcgca gccgcagcgg gccgatcgaa agcgctgctg
     6421 gctgctcgac cgtgaccgtg aaaggcgggc gttgcaggtc gagcagaaga gcggtctgca
     6481 acagccgcag cagccgctgg cgttcgatct gccacaaacg accggcgccg gccgtacggt
     6541 gatgctgctg catcaccgca tcgactgcct gctggcacca ggcgtcgcgc tgggctgcat
     6601 cggcatgctg cagtcgttcc tgcgaacgca cctcgctcca gaatcgctcc atcagcagat
     6661 ggctgaaggt gccgcgttcg cgggcatcga aaccgagctg gggtggttcg gccggcagca
     6721 gtcgcagccg atggttggca taggcgcgaa acgggcaggc ggcctgatcc ttcagcagtt
     6781 gcgtgccgcc acggctgggg cggtcgtccg tcagtaccag cggcagaccc tgatgatctt
     6841 cagtgggcag ggtggccagc ggctgctggc gcaacggctc gatggccgag gtgacaaggc
     6901 ccagcgccgc cgccgtggtg gtcggccagc cgctgatcag cgggctcggt tcgagcggtt
     6961 cttcatcctt gcggcaggca tggctcagga tcacctggtc ggcggcgccg agcaggcgcc
     7021 gggtgatgcg ggcggcaaat tcgatctcgc gctcggcagt ggcgcgcggc agatccagcc
     7081 gccgctgcaa gccgatcggc agcagcggat tgggtgccgg cgcggtgggc cagttgccac
     7141 gatcgagccc catgatccag agctggtcga actgcaaacc gctggcttcg agcaggccca
     7201 gaatctggat tggactgtcg ccactttgcg gctgaaagct ggcgagcgcg agcagttcgg
     7261 tcagccgctg caacgccgtc cccgcagcga ggggtgcggt gtaggggtcg agcatggcaa
     7321 agcgttgcag tgcctcgcgc caggtttcga ccgcctgtaa ctcctcgctg tcgagcgtgc
     7381 gttcacccgg ccagcccagc gccagcaacg cgctgctgaa ccggcgtgcc cagtacgagg
     7441 gtggttgtgc ggcactgccc tgctgtgcct gatcgagcag atcgatcagg gtgctgctga
     7501 acgggctggc atggctgttt ggcaactgca gcagggctgc gcgcagcaga ggtggcgacc
     7561 actcgtgacg gcgctgctgc tgcagggcag cttcgaactg cgcctgtcgt ctctcctcgc
     7621 cgatgaatgg agagcgcaga atctgccgca gcgcggtggc gctgaggtcg gtgccagtgg
     7681 tcagcctcag caacgtcagc gcatcggcga tgatcggtac cgcaggcagt ggcagcggcg
     7741 cggagaggtt gaccggtggc gtgtagggcg gcgtgctggg cagcagcgca tggggttcga
     7801 gctgttcccg cagcgcatga agcaactggc cacgcacgcg cggcaattcc ggcgccacca
     7861 cgccgattcg caccctctcg ccccgttcga tcaacactcg gcggcaccag gcggcgatgg
     7921 cctgcaattc atcacgctcc tggtcgcagg gcaaccgttg cggctggctc gggcgtggat
     7981 cggcatcgag ctgtcgaagg gtggcacccg cggcattggc tcgatcgagc agcctttgcc
     8041 aggccggcgg ccattcgtca aaagcgtaga gcagcagctg tctgggcaag ctgatctggc
     8101 cgtggtcgat cgccgtggtg atcagtgcgg gcagctcggc actgctgatc cagccctgtt
     8161 cacgacagcg gccgcgaaag tgggcaaccc actgcgcaag ccagccaaag ctttcatcca
     8221 cggccaactc attgatcaga cgcgactcag agacttgcca cagttgcagc agttcccagg
     8281 cgtcgctggc ctgtaccgcc agatcggcgg cccggaacag cccttggtca ggcagtgccg
     8341 actcgacggc ctgctgccac agcagccgct gcgcccactc atccagcagc gtggcctgcc
     8401 agcgccgatc actgctgagg gcctgttgct ggagatagag ctgctggagc caaccgctga
     8461 atggcaggat cgcggggctg cgccaggcct tgacgccccg ttcgcgctgc aactgggcgt
     8521 ggcgattcag cagtgtgcgc gacagccgaa gattgggcgt caacagcagc gagtggtggt
     8581 cgagagcggc cagggtggca tcgggctgtg acatcgtcat cctgtgcttg cgcggtttcc
     8641 gcggtgtggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgatggaggc
     8761 gggacatggg cagcagtgag tgcgccggcc tcagcgcgcc acttggctca gcaccagttg
     8821 caccacccca tacacggtca gcacaaagag cacggtaaaa atgacgccgg caatgatgaa
     8881 ccggctcgcc ttgccatggg cgaaatcccg ttcaagattg gcgcgcttct gcacacccag
     8941 tgccgccgcc agcacgctgc cgatggtttg caggatcgac agcgattcgg gttgtttcga
     9001 aggctctttc tttgattcgt tcatgcaact ctcctgatga ggcgagcgct gattataccc
     9061 cgcctgtcca gagggccacg ctgctctgaa gaccatcttt ctggagccgt ttgcgccggg
     9121 ctgtcatcgt cgcctcgatg cagtacagtg acgaccgcat cgacactgtg aacccaatcc
     9181 gttcaggagc cgactcatgg agaccctgcg cccgttctcg atcgagattc ccgaagcgca
     9241 actgatcgat ctgcggcagc ggttggccgc gacacgctgg cccgaggccg agacggtcga
     9301 tgactggtcg cagggggtgc ctctggccta tctgcgggag ttttgcagtt attgggcgca
     9361 agagtatgac tggcgcgtga ccgagcgacg gctcaacgtc tttccccagt tcaagagcca
     9421 gatcgatggg ctcgacatcc attttctgca tgtgcgcagt cgccatgcgc aggcgctgcc
     9481 gttgctgatc acccatggct ggcctggttc catcgtcgag ttccacaagg tgattgcccc
     9541 gctgagcgat cccaccgcat tcggcggcag cgccgacgat gccttccatg tcatctgccc
     9601 ctcgctgccg ggttatgcct tttcagacaa accgcgtcag acaggctggg gggttgagaa
     9661 gatcgccacg gtctgggatg ccctgatgcg ccgactcggc tatcagcgct atgtggcgca
     9721 aggaggggac tggggcgctg cggtcaccac cgccatcggc acgcagaatc tcggcgcctg
     9781 catcggcatt catctcaaca tgccgactgc ctcgccgacc ccgcaggcgt tgagcagtcc
     9841 gaccgagcgt gatcagcgcg cactggccgc cgcggcccac tatcaacagt gggagggtgg
     9901 ctactcgaag cagcaggcca cccggccaca gaccctcggc tatgggctgg tcgattcgcc
     9961 ggtcgggcag gccgcctgga ttcttgaaaa gttccgggcc tggaccgact gtcagggaca
    10021 ccctgaaaac atcctcagcc gcgatgaact gctcgacaac atcatgctct actggctgac
    10081 cgcctcggcg gcctcatcag cccggctcta ctgggagagc ttcggcagct tcgggggcga
    10141 ccgatcggcg ggcgtcgaac tgccgaccgg ctgcagcatc tttcccaaag agatcgtgcc
    10201 gacgccgcgc agttgggccg agcagcgcta ccgcaacatc gtctactgga atgagctcga
    10261 tcgtggcggc cattttgctg ccttcgagca gccgcagcgg ttcgtcgaag agctgcgtgc
    10321 ctgctttgcg ctgatgcgct gagggggtga catggctttg gaaaagaaga ttcgcggcgt
    10381 caacctcggc agttggctgc tgctggagaa gtggatggta cccagtctgt ttgccggact
    10441 ggaggccacc gacgagacca gttggtgcgt cgagctgggt gatcgggccg ccgagccgct
    10501 gcatcggcat tggaacagct tcatcacccg cgatgacttc gtctggctgg ccgcgcgtgg
    10561 catcaacgcg gtgcgcattc cggtgggcca ctggattttc ggacctgact acccctacca
    10621 ccgcagctat ggcgcccacc gccacccttt cgtgaccggt ggcatcgaac tgctcgacc
//
LOCUS       DAORCQ010000079        10412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4343_length_10412_cov_5.867915, whole genome shotgun sequence.
ACCESSION   DAORCQ010000079 DAORCQ010000000
VERSION     DAORCQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4343_length_10412_cov_5.867915"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PKI23_08685"
     CDS             <1..1253
                     /locus_tag="PKI23_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37051.1"
                     /translation="CHNWPRWRLPWATRGEWWLRRPARSGPMIGLGLVTLWLALLLGW
                     GWRLRGALRVLWCEPCLSRPVLIFESDDWAPAPPTQQAALNRLIDTLAAFRDAVDHPP
                     VMTLGLVLSAPVQQAGQLQIRQFDEPAAEPLRAVIQRGIDAGLFSPQLHGAAHYWPAA
                     FADWLAGDSAAGQWWGDAANLRTETLPSALQSRWWNPRLDPPAAPAAAEAEAAAEAEA
                     TLFQRCFGEPPRVAVPPTFLWQEAVETGWRRGGVRHVVTPGRRWQARDATGRLRDAGV
                     VLHNGQRSRQGLGYLVRECYFEPERGHTAEDGLRALTQQSRLARPTLLEIHRSNFIGD
                     SALCERALQELQRLLEQALRHHGELRFLSTLELAERIDRNDADWMLHQPLQRLKFFVR
                     RGAIEPGLSRWFKLSGLFLLAGRG"
     gene            1259..2218
                     /locus_tag="PKI23_08690"
     CDS             1259..2218
                     /locus_tag="PKI23_08690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN37052.1"
                     /translation="MGSPSFSVIIPVYNGAATLARAIDSVLAQRHPAHEIIVVDDGST
                     DDSGRVAAGYGSAIRYLRQPNQGVSAARNQGAALASGDWLAFLDADDWYHPCRLEAHA
                     RLLERAPDVGFMTGDFDYFDDAGNFLRRSMVSTPLGRQLLQQADAAGQAFFGGEQIAS
                     FIEAHFGDTHTLSVRRDWFIELGGYPPGLPVCEDVHFLIRLCARAQRIGVHCQPLAVY
                     LIHGASATRRDPVRAQAETVRALSAIAGLEQMPRWIRTGVAGARRHARFDWAMALLRR
                     GQRRQALAAVLPLLSEQPSWASLRQVASVARGCFQNLHSRTEP"
     gene            2215..3378
                     /locus_tag="PKI23_08695"
     CDS             2215..3378
                     /locus_tag="PKI23_08695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNN37053.1"
                     /translation="MNHPLLVITELFLPTKGGTAVWFDEVYRRIGGKEIHVVTAAVPG
                     SDEHDRDHPNSIHRLRLQRHPWLRPESLAMYLKLLGSSLKLAFTQPVVAVHAGRVLPE
                     GLIGWLVAVCRRVPLLIYAHGEEITTWTQPLKRRVMRFSYRRADCIIANSRFTFDELC
                     KLGVDPARIRTLFPGVDIERFRPGLPAADLRQSIGVGPGQPLLLSVGRLSRRKGFDQV
                     IRALPALIEAGFDPHYAIIGIGEEHDPLARLASELQVRDRLHLLGHVPTDTLPRWYNG
                     CELFVMPNREVDGDTEGFGMVFLEAAACGRTAIAGLAGGTGDAVVDGETGLRIDGAEL
                     PALTAAIATLLEDATLRQQLATQALSRARRDFSWHSVAQKTVQLLPNGRRNPE"
     gene            3356..3931
                     /locus_tag="PKI23_08700"
     CDS             3356..3931
                     /locus_tag="PKI23_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN37054.1"
                     /translation="MVGATLNEERAMKRMRYCLGLLWFFSMAVMAQDFARTAEENAMC
                     VAVINQGRDTSDQANWVHMHHYCDCLRFTQRAVSSANEAQRRDNLGQAVDGCNYVLGH
                     VSENFYMRPMVLVSRGRALELAKRPAEAIADYETAIRLNPSYYKAYIGLSDAYMAVGD
                     RKAALQAVERGLQVAPQSTGLQKRLAKLKGS"
     gene            complement(3918..7064)
                     /locus_tag="PKI23_08705"
     CDS             complement(3918..7064)
                     /locus_tag="PKI23_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNN37055.1"
                     /translation="MSASLTVFICTHNRAELLRRTLHHLNQARRPAISVSILVIANAC
                     SDDTQRLLEAYQAEAAEQDWLALDWHAEPRPGKSHALNCGLALIESTLVAFVDDDHRV
                     AEDFLVETVKAAAAFPETTLFCGRILPDWDGSEPAWVHDEGPYRIYPLPVPCFDCGDA
                     GFEIDAQGRLPGGGNLFARTEALRRTGRFSIELGPKGHDLGGGEDTEFIARALNQGER
                     LRYIPTVVQHHFVEHERLELRYLLRKSYQRSKAAMQIQAPSRQIPRYMVRKLVEHGGR
                     ALFSLNVSRSRFYLMRVAATLGEIAGTRAARQQRPSWCSFDRPLRWPLWLAAIGTGLT
                     GMALTRPSLESEAVAALTSSAVMALLLTLALVTKSIIDFSQTGPQIRDEVTGAFRFYT
                     LYAMGRLAFWCWLILTLTGSLGAAGWMAVSTLQQADVPGPMALGAAALLSSGMVVLGR
                     FCHLLLHRPAALAASAHYRLSRLYPLWRRLSPARLRHAATLLLLIPTGLLTAAAIVAL
                     LRGEPALAALHGGLLLTLTGIAGWSFLPRALQSGPLRTGAPMNLLMIGVDTLRSDRIG
                     RLRHGKPLSPTLDALAERGLFYPNCYTPCARTAPSLISLLTGTWPHRHGVRDNFVDDG
                     QTQLPVTALPALLQAAGYHTAAISDWCGADLGKFSFGFEQVDLPEDQWNLKYLIRQGP
                     KDLRLFLSLFTHSHFGRRFLPELYYLAGIPLTRHLSQRACHAIDQLAGQPKPFFLNLF
                     LSTTHPPFGSEYPHYISQSDPAYQGESKFVMTRLTDPFEIIARQGEAREEFDLDQIID
                     LYDGCVANLDAELARILRHLQQRGLTQNTLLVLYSDHGMEFFEHDTWGQGNSAIGEAS
                     PRTPLILVDPRQPARGKSAAVVRSIDLLPTLLELLGITAADEIDGVSLAAHLTEGRPP
                     AMLDAFNETGIWLTPPPGMPADHLRYPDLLELIEVADKHSGTLGIKPDYRQQIVLAKD
                     RMLRRGHWKLVHQPLQQGARQLLFDLERDPHCQHDLAAQHPEVVAQLWPRLDGWIMAD
                     PLMRTAPGPAGDGAVRSP"
     gene            complement(7091..8032)
                     /locus_tag="PKI23_08710"
     CDS             complement(7091..8032)
                     /locus_tag="PKI23_08710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN37056.1"
                     /translation="MSASTIQASVIVCTYNRAESLRHTLNALAAQQLPSTVEWELIVV
                     DNNSSDQTREVVLAAQAQFATLHYLHEPRQGLSFARNCGIAAARGEVLLFTDDDVRPE
                     PDWIATVLAALEREQAEACGGYIAPDWEVAPPAWLTERFYGFLAIKTETAACRIDTLD
                     RLPFGANMAFRRQLFDRFGRFDTERGRKGNQLASGEDGELFARLIRAGIKVIHVPEAR
                     VHHRIEAFRVEKRYFRRWRSHTSRNLAQTVGLPGERRLAGIPLYLFAQLARAAWRTLV
                     GYLTAPADEAFHREVVLWHFIGTLDGLWRSRHRPVQP"
     gene            complement(8029..8739)
                     /locus_tag="PKI23_08715"
     CDS             complement(8029..8739)
                     /locus_tag="PKI23_08715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNN37057.1"
                     /translation="MSPQSFAEQMAKLHKKGYQAVGIDGFMAWFRGEATLPEKAFLLT
                     FDDGFFDLDRHVADLLQEFQWPAAIFLVTGLMGQTDEWMQSQGTSAVHRLLDWEQVEV
                     LKTLGFTVHAHSRSHRDLTLLDDEQLASEVEGAKRDLAEHGFDAHFFAYPYGRCDARV
                     IAAVREAGYEAAFCNQCGFNRPQRDPFQIRRIEVHGSDSPVNLLRKIELGTNDGSRRA
                     RARYYQSRLQARIRPGSG"
     gene            9084..9407
                     /locus_tag="PKI23_08720"
     CDS             9084..9407
                     /locus_tag="PKI23_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNN37058.1"
                     /translation="MNMSIRIEELLQSGLAPAYLEVVDESQQHNVPPGAQSHFKVTVV
                     SDQFAQKNLVARHRQIYALLREPLAGGVHALALHTYSLAEWQARGGGSLDSPRCLGGS
                     ANEAR"
     gene            complement(9415..>10412)
                     /locus_tag="PKI23_08725"
     CDS             complement(9415..>10412)
                     /locus_tag="PKI23_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNN37059.1"
                     /translation="AGIPAMMLFQLLRNLNEGLGLPRLAMRVGTVAFALNIPLAWCLV
                     TGEAGLPALGGAGCGIATALLFWVMFALLWLLSRRDARIAPHLRGTVRLDGALLREFL
                     QLGLPIGVGLFLESGLFALVALLLAPLGAVAVAAHQVALNIAATLFMLPLSLALALTV
                     RLGHHLGASAHPAAHRTLRVALAMAAVIATGNTLLILLLGEPLVALHSGDAAVRALAL
                     RLLCYAALFQLSDCMQMTLLGALRALGQTRATMWIAFFVYWGVTLPLGYSLGIADWLG
                     PPRGAEGLWIGLTLGITLAAVLLGWRLRQSSLFDNTRAQTWKGPPRTLSDPSGSS"
BASE COUNT         1763 a         3405 c         3532 g         1712 t
ORIGIN      
        1 gttgccacaa ctggccgagg tggcggctgc catgggctac ccgcggtgag tggtggctgc
       61 ggcgacccgc gcgaagcggg ccgatgatcg ggctggggct ggtcaccctc tggctcgcac
      121 tgctgctcgg ctggggctgg cgtctgcgtg gcgcgcttcg ggtgctgtgg tgcgaaccct
      181 gcctgagccg ccccgtgctg atcttcgaga gcgacgactg ggcgccggcg ccgccgaccc
      241 agcaggcggc gctgaaccgg ctgatcgaca cgctcgctgc ctttcgtgat gccgtcgacc
      301 acccgccggt gatgacactg ggtctggtgc tgagcgcgcc ggtgcagcag gccgggcagc
      361 tccagattcg ccagttcgac gaaccggccg ccgaaccgct gcgggcagtg attcaacgcg
      421 gcatcgatgc cggactcttc tcaccgcaac tgcacggcgc ggcccactac tggccggcgg
      481 cctttgcaga ctggctcgcc ggcgattcgg cggcgggtca atggtggggc gatgccgcca
      541 acctgcgcac tgaaacactg ccctcggcgc tgcagagccg ctggtggaac ccgcggctcg
      601 atccgccggc cgccccggcg gcggccgagg ccgaggcagc ggccgaggcc gaggccacac
      661 tgttccagcg ctgcttcggc gagccaccgc gggtggcggt gccaccgacc tttctgtggc
      721 aggaggccgt cgagacgggc tggcgccgtg gcggagtgcg ccatgtcgtc accccggggc
      781 ggcgctggca ggcacgcgat gccaccggtc gactgcgtga cgccggagtc gtgctgcaca
      841 acggtcagcg cagccggcag gggctcggct acctggtgcg ggagtgctac ttcgagcccg
      901 agcgcggcca caccgccgag gatggcttgc gggcgctgac gcagcagagc cgcctggcgc
      961 ggcccacgct gctggagatc caccgcagca acttcatcgg cgacagcgcg ctgtgcgaac
     1021 gcgcgctcca ggagttgcaa cggttgctgg agcaggcgct gcgacaccat ggtgaactgc
     1081 gctttctctc caccctcgaa ctggccgagc gaatcgaccg caacgatgcc gactggatgc
     1141 tccatcagcc actgcaacgg ctgaaattct ttgtgcgccg cggcgccatc gagcccggcc
     1201 tcagccgctg gtttaaactg agcggactgt tcctgctggc ggggcgcggg tgatgacgat
     1261 gggcagcccc agcttctcgg tgatcattcc ggtctacaac ggtgccgcca ccctggcgcg
     1321 cgccatcgac tcggtgctgg cgcagcgcca tccggcgcac gagatcatcg tcgtcgatga
     1381 tggctcgacc gacgactccg gcagggtcgc cgccggttat ggcagcgcga tccgctacct
     1441 tcgccagccg aaccaggggg tctcggccgc gcgcaaccag ggtgccgcgc tggccagcgg
     1501 cgactggctg gcctttctcg atgccgatga ctggtaccac ccctgccggc tcgaagccca
     1561 tgcccggctg ctggagcgcg cccccgatgt cggcttcatg accggcgatt tcgactactt
     1621 cgacgatgcc ggaaacttcc tgcgccgttc gatggtcagc accccgctcg ggcggcagct
     1681 gctgcaacag gccgatgcgg cgggccaagc ctttttcggt ggcgagcaga tcgcctcctt
     1741 catcgaagcc cactttggcg acacccacac cttgagtgtc aggcgtgact ggttcatcga
     1801 gcttggcggc tatccgcccg gcctgccggt ttgcgaggat gtccacttcc tgatccggct
     1861 ctgcgcccgc gcgcaacgca tcggcgtgca ctgccagcca ctggcggtct atctgattca
     1921 tggcgccagc gcgacccggc gcgatccggt gcgggcgcag gccgagacgg tgcgggcgct
     1981 ctccgccatc gccgggctgg aacagatgcc gcgctggatc cggaccggtg tcgccggcgc
     2041 ccgccgccat gcccgcttcg actgggcgat ggccctgctg cgccgcggtc agcgtcgcca
     2101 ggcgctcgct gccgtcctgc cactgctcag cgaacagccg tcatgggcca gtctgcggca
     2161 ggtggcctcg gtggcacgcg gctgcttcca gaacctccat tcgcgaaccg agccatgaac
     2221 cacccgctgc tggtcatcac cgaactcttt ctgcccacca agggcggcac cgcagtctgg
     2281 ttcgacgagg tctaccggcg catcggcggc aaggagatcc atgtcgtcac cgccgccgtg
     2341 ccgggttcgg acgagcatga ccgcgaccac cccaactcga tccatcgact gcggttgcag
     2401 cgccacccct ggctgcgtcc cgagagtctg gcgatgtacc tgaagctgct cggcagcagc
     2461 ctgaagctgg cattcaccca gccagtggtg gcggtccatg ccggccgggt gctgccggag
     2521 gggttgatcg gctggctggt cgccgtctgc cggcgcgtgc cgctgctgat ctacgcccat
     2581 ggcgaagaga tcaccacctg gacccagccg ctgaagcgcc gggtgatgcg cttcagctac
     2641 cggcgggccg actgcatcat cgccaacagc cgcttcacct tcgacgagct gtgcaagctg
     2701 ggggtcgatc cggcacggat ccggaccctc ttccccggtg tcgacatcga gcggtttcgc
     2761 cccggactgc cggcggccga tctgcgccag tcgatcggtg tcggcccggg gcagccactg
     2821 ctgctgtcgg tcggccggct cagccggcgc aaggggttcg atcaggtgat ccgggcgctg
     2881 cccgcgctga tcgaggccgg tttcgacccg cactatgcca tcatcggcat tggcgaggag
     2941 catgaccctc tggcccggct ggccagcgaa ctgcaggtgc gcgaccgcct gcacctgctg
     3001 ggccatgtgc cgaccgacac cctgccgcgc tggtacaacg gctgcgagct gttcgtgatg
     3061 ccgaaccgcg aggtcgatgg cgacaccgaa ggcttcggca tggtctttct cgaagcggcc
     3121 gcctgcggtc gcaccgccat cgccggactg gccggcggca ccggcgatgc cgtggtcgat
     3181 ggcgagaccg ggctgcgcat cgatggcgcc gaactgccgg cgctgacggc ggccatcgcg
     3241 accctgctcg aagatgccac gctgcgccag cagctggcca cccaggcgct cagccgtgcc
     3301 cgacgggatt tttcatggca cagcgtggcg cagaagaccg tgcagctgct gccgaatggt
     3361 cggcgcaacc ctgaatgagg agagagcgat gaagcgtatg aggtattgtc ttggcctgtt
     3421 gtggtttttt tccatggccg tgatggcgca ggattttgcc cggactgctg aagagaacgc
     3481 catgtgtgtc gcagtgatca accagggccg cgacacctcc gatcaagcta actgggtgca
     3541 catgcaccac tattgcgact gtctgcgttt tacccagcgc gcagtgagca gtgcgaatga
     3601 ggcgcaaaga cgggacaatc tcggtcaggc agtcgatggc tgcaactatg ttctggggca
     3661 tgtcagtgag aatttctaca tgcgaccgat ggtgctggtt tcccgaggac gtgcgttgga
     3721 gttggccaaa cgtcccgccg aagccattgc cgactatgaa accgccatcc gtctcaaccc
     3781 gagctactac aaggcctata tcggcctgag cgatgcctac atggctgtcg gtgaccgcaa
     3841 agcagcgctg caggcagtcg agcgtggcct tcaggtcgcg ccgcaatcta ccggattgca
     3901 gaaacggctg gccaagctca agggctcctg acggccccat ccccagccgg tcccggcgcc
     3961 gtccgcatca gcggatcggc catgatccag ccatcgagcc gcggccacaa ttgcgccacc
     4021 acctcggggt gctgcgccgc caggtcatgc tggcagtgcg ggtcgcgctc caggtcgaac
     4081 agcagctggc gcgcgccctg ttgcagcggc tgatggacca gtttccagtg gccgcggcgc
     4141 agcatgcggt ccttggccag cacgatctgc tgccgataat cgggtttgat accgagggtg
     4201 ccgctgtgtt tgtctgccac ctcgatcagc tcgagcaggt cggggtagcg caggtggtct
     4261 gccggcatcc ccggtggagg agtcagccag atgccggttt cgttgaaggc gtcgagcatg
     4321 gcgggcggtc tgccctctgt cagatgggcc gccagcgaca cgccatcgat ctcgtcggca
     4381 gcggtgattc ccagcagttc gagcagcgtt ggcagcagat cgatgctgcg caccaccgcc
     4441 gctgattttc cgcgagctgg ctggcgcgga tcgaccagga tcagcggtgt gcgcgggctg
     4501 gcctcgccga tcgccgagtt gccctggccc caggtgtcat gctcgaagaa ctccatgcca
     4561 tggtcgctgt agagcaccag cagcgtgttc tgcgtcaggc cacgctgttg cagatggcgc
     4621 aggatgcgcg ccagttcggc atcgaggttg gcgacacagc catcgtagag gtcgatgatc
     4681 tgatcgaggt cgaactcctc gcgcgcctcg ccctgccggg cgatgatttc gaagggatcg
     4741 gtcagccggg tcatcacgaa tttcgactcg ccctggtagg ccggatcgct ctggctgatg
     4801 tagtgcggat attccgaacc gaagggcgga tgggtggtgg agagaaacag gttgagaaag
     4861 aacggcttgg gctggccggc cagctggtcg atggcatggc aggcgcgctg gctcagatgg
     4921 cgggtcagcg ggatgccggc caggtagtag agctccggca gaaagcgacg gccaaagtgg
     4981 ctgtgggtga agagcgacag gaacagccgc agatccttgg gcccctggcg gatcagatac
     5041 ttgaggttcc attgatcctc cggcagatcg acctgctcga agccgaagct gaatttgccg
     5101 aggtcggcgc cacaccagtc ggagatggcc gcggtgtggt agcccgccgc ctgcagcagc
     5161 gcgggcagcg cggtcaccgg cagttgcgtc tgcccatcat cgacaaagtt gtcacgcacg
     5221 ccgtggcggt gtggccaggt gccggtcagc agcgagatca ggctcggtgc ggtgcgggcg
     5281 cagggtgtgt agcagttggg gtagaacagg ccgcgctcgg ccagtgcatc gagggtgggg
     5341 ctcaacggct tgccgtggcg cagccggccg atccggtcgg agcgcagggt gtcgacaccg
     5401 atcatcagca ggttcatcgg tgcgccggtg cggagcgggc cgctctgcaa tgctctgggc
     5461 agaaagctcc agccggcaat gccggtgagg gtcagcagca gaccaccgtg gagcgctgcc
     5521 agggccggtt cgccacgcag gagggcgaca atggccgctg cggtcagcag cccggtcggg
     5581 atcagcagca acagggtggc ggcatgccgc aatcgcgccg gcgacagccg tcgccacagc
     5641 ggatagagcc ggctcagccg atagtgcgca gaggccgcca gcgcggccgg tcgatggagc
     5701 agcaggtggc agaagcggcc cagaaccacc atgccgctcg acagcagcgc ggcagcgccc
     5761 agcgccatgg ggcccggcac gtccgcctgc tgcaaggtgc tgaccgccat ccagcctgcc
     5821 gcgccgaggc tgccggtcag ggtcaggatc agccagcacc agaaggccag ccgtcccatc
     5881 gcatagaggg tgtaaaagcg aaaggcgccg gtgacctcgt cccgaatctg cggtccggtc
     5941 tggctgaagt cgatgatcga cttggtcacc agcgccagcg tcagcagcag cgccatgacc
     6001 gcgctgctgg tcagcgccgc cacggcttcg ctctccagtg atggccgggt cagggccatg
     6061 cccgtcagac cggtaccgat tgcggccagc cacagtggcc agcgcagcgg ccggtcgaat
     6121 gaacaccatg acggacgctg ctgtcgggca gcgcgtgtgc cggcgatctc gcccagggtg
     6181 gcggcgaccc gcatcagata gaagcggctg cgggaaacat tgaggctgaa cagtgcccga
     6241 ccgccatgct ccaccagctt gcggaccatg tagcgtggaa tctgccgcga tggcgcctgg
     6301 atctgcatcg ccgccttgga gcgctgatag cttttgcgca gcagatagcg caactccagc
     6361 cgctcatgct cgacgaagtg gtgttgcacc acggtcggaa tgtagcgcaa ccgttcgccc
     6421 tgattcaggg cgcgggcgat gaactcggtg tcctcgccgc cacccagatc gtgtcccttc
     6481 ggccccagtt cgatgctgaa tctgccggtg cgtcgcaggg cctcggtgcg ggcgaacagg
     6541 ttgccgccgc cggggagccg accctgcgcg tcgatttcga atcctgcatc gccgcagtcg
     6601 aagcagggca ccggcagcgg atagatgcgg tagggtcctt cgtcgtgcac ccatgccggt
     6661 tcactgccat cccaatcggg caggatcctg ccgcagaaga gggtggtctc gggaaaggcc
     6721 gcggcagcct tgacggtctc gaccagaaaa tcctcggcca cgcggtggtc atcgtcgacg
     6781 aaggccacca gcgtgctctc gatcagcgcc agcccgcagt tgagcgcatg ggatttgccc
     6841 gggcgcggtt cggcatgcca gtcgagcgcc agccaatcct gctctgcggc ctctgcctga
     6901 taggcctcga ggaggcgctg ggtgtcgtcg ctgcaggcgt tggcaatcac cagaatcgac
     6961 actgaaatcg ccggtcgccg cgcctgattc aggtggtgca gggtccggcg cagcagttcg
     7021 gcccggttgt gggtgcagat gaagaccgtc aacgaagctg acatgagcgc atccttgccc
     7081 ggtcagacct tcagggctgt accggccggt ggcggctgcg ccacaggcca tcgagggtgc
     7141 cgatgaaatg ccacagcacc acctcccggt ggaaggcctc atcggccggt gccgtcaggt
     7201 agccaaccaa tgtgcgccag gccgcgcggg ccagctgggc aaacagatag agcggaattc
     7261 ccgccagccg tcgctctccc ggcagcccca ccgtctgtgc caagttgcgg ctggtgtgcg
     7321 agcgccagcg gcggaagtag cgcttttcga cccggaacgc ctcgatgcgg tgatgcaccc
     7381 gcgcctcggg cacatggatc accttgatgc cggccctgat cagccgtgcg aacagctcgc
     7441 catcctcgcc gctggccagt tggttgccct tgcggccgcg ctcggtgtcg aaccggccga
     7501 aacggtcgaa cagctggcga cgaaaggcca tgttggcgcc gaacggcaat cggtcgagcg
     7561 tgtcgatccg acaggctgcg gtctcggtct tgatggccag aaaaccgtaa aaacgctcgg
     7621 tcagccaggc tggcggggcg acctcccagt cgggcgcgat gtagccacca caggcctcgg
     7681 cctgttcgcg ctcgagcgcg gccagcaccg tggcgatcca gtcgggttcg gggcggacat
     7741 cgtcgtcggt gaacagcagc acctccccgc gtgccgccgc gatgccgcag ttgcgggcga
     7801 acgacagccc ctgtcggggt tcatgcagat aatgcagcgt ggcgaactgc gcctgcgcgg
     7861 ccagcaccac ctcgcgggtc tggtcgctgg agttgttgtc gaccacgatc agttcccact
     7921 cgaccgtaga cggcaactgc tgtgcggcca gcgcgttcag ggtgtggcgc agcgattcgg
     7981 cacggttgta ggtgcagacg atgacgctgg cctggatggt ggaggcgctc atccggaccc
     8041 cggcctgatt cgcgcctgca gacggctttg gtagtagcgt gcccgtgccc ggcgcgagcc
     8101 atcattggtg cccagttcga tcttgcgcag cagattgacc ggtgagtcgc tgccatgcac
     8161 ttcgatgcgg cggatctgga aaggatcgcg ctgcggccgg ttgaagccac actggttgca
     8221 gaaagccgcc tcgtagccgg cctctctgac cgctgcgatg acccgtgcat cgcagcgccc
     8281 gtaggggtag gcaaagaagt gggcgtcaaa gccatgttcg gccagatcgc gctttgcgcc
     8341 ctcgacctcg ctggcgagct gttcatcatc aagcagcgtg agatcgcggt ggctgcggga
     8401 gtgggcgtga accgtgaacc ccagcgtctt cagcacctcg acctgctccc agtcgagcag
     8461 tcgatgcact gccgatgtgc cttggctctg catccactcg tcggtttgcc ccatcagacc
     8521 ggtgaccaga aaaatcgccg ccggccactg aaactcctgc agaagatcgg ccacatggcg
     8581 gtcgagatca aaaaaaccgt catcgaaggt gagcaggaat gccttttcgg gcaaagtggc
     8641 ctcgcccctg aaccaggcca tgaaaccgtc gatgccgacc gcctgatagc ccttcttgtg
     8701 cagtttggcc atctgttcgg caaagctctg cggtgacacg cagtgagaac gctcctggcg
     8761 accggccgcg cgaccgatcc ggcggtactt caagaccggc acccgttcac tgttcattga
     8821 caaggaagga tggctcttgc agaggatgga agatggcctg aagggtgcgt caaccggctg
     8881 gccatgatgc cgcgatcgcg ctctgctgct tcggaagtgg tcacaggtcg aacatctgct
     8941 cgccgcgttg aaagctgcgg ccaattatag gtgcatcggc cagaaaaggc gcaattccgc
     9001 tgaatgagcg cgaagctggg catcgggacc ggatttgcgt taacgtgcga accgttgcca
     9061 atttctgtcc acaggagcgc acgatgaaca tgtcgatccg catcgaagag ctgttgcagt
     9121 ccgggttggc gcccgcatac ctcgaagtgg tcgatgagag ccagcagcac aatgtcccac
     9181 ccggtgcgca gagccacttc aaggtcacgg tggtcagcga tcagttcgcg cagaaaaacc
     9241 tggtggcccg tcatcggcag atctatgcgc tgctgcgcga accgctggct ggcggtgtgc
     9301 atgcgctggc gctgcacacc tactcgctgg ccgaatggca ggcgcgtggc ggtggttcgc
     9361 tcgactcgcc gcgctgtctg ggcggttcag ccaacgaggc cagatagcgg aggctcatga
     9421 cgatccgctc ggatcggaca gcgtacgagg cggacccttc caggtttgcg cacgcgtgtt
     9481 gtcgaacagc gaggattgcc gcagacgcca ccccagcagc actgccgcca gcgtgatgcc
     9541 cagtgtcagg ccgatccaga gcccctcggc cccacgtggc gggcccagcc agtcggcgat
     9601 gccgaggctg taacccagcg gcagggtgac cccccagtag acgaaaaagg cgatccacat
     9661 ggtggcgcgg gtctgtccga gtgcgcgcag cgcccccagc agggtcatct gcatgcagtc
     9721 cgaaagctgg aacagcgccg catagcagag caggcgcaac gccagcgccc ggacagctgc
     9781 atcgccactg tgcagcgcca ccagtggctc gcccagcagc aggatcagca gcgtgttgcc
     9841 ggtggcgatg acggcggcca tcgccagggc cacccgcagc gtgcgatggg cggccggatg
     9901 agcgctggca cccagatggt gtcccagccg caccgtcagt gccagggcca ggctgagggg
     9961 gagcatgaac agggtggccg cgatgttcag cgccacctga tgggcggcga cagccaccgc
    10021 gcccagcggc gccagcagca gcgccaccag tgcgaacagg ccgctttcaa gaaaaagtcc
    10081 aacgccgatc ggcaggccga gctgcaggaa ctcccgcaac agcgcgccat cgagacgcac
    10141 ggttccgcgc agatggggcg cgatgcgcgc atcgcgccgc gacagcagcc agagcagcgc
    10201 gaacatcacc cagaacagca gcgccgtggc gatgccgcag ccggcgcccc ccagtgccgg
    10261 cagtcccgct tcacccgtca ccaggcacca ggcgagtggg atgttgagcg cgaaggcgac
    10321 cgtgccgacc cgcatcgcca gtcggggcag tcccagtccc tcgttgaggt tgcgcagcag
    10381 ctggaacagc atcatcgccg gaatgccggc ac
//
LOCUS       DAORCQ010000080        10061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4577_length_10061_cov_5.172796, whole genome shotgun sequence.
ACCESSION   DAORCQ010000080 DAORCQ010000000
VERSION     DAORCQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4577_length_10061_cov_5.172796"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PKI23_08730"
     CDS             <1..146
                     /locus_tag="PKI23_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNN37060.1"
                     /translation="FMHCLPAHRGEEVSEEMLDDHRSVVWEEAENRLHAQKALMEWLL
                     QQH"
     gene            275..961
                     /gene="phoB"
                     /locus_tag="PKI23_08735"
     CDS             275..961
                     /gene="phoB"
                     /locus_tag="PKI23_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNN37061.1"
                     /translation="MHGQKILVVEDEPEIRDMLAFSLERGGYAVTLAEDGESALRLLD
                     QQRPDLILIDWMLPGLSGIELANRLRRDEPTQELPLIMLTARGEESDKLKSFSVGIDD
                     YITKPFSSKELLARIKALLRRSGRGDEGRLAIDGLCLDTQGHRLLVDGKPVHIGPTEY
                     RLLELFMSNPDRVYNRDQLLDRVWGRTVYVEERTVDVHILRLRKTLAPHGYDRLIQTV
                     RGTGYRLSRT"
     gene            964..2334
                     /gene="phoR"
                     /locus_tag="PKI23_08740"
     CDS             964..2334
                     /gene="phoR"
                     /locus_tag="PKI23_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNN37062.1"
                     /translation="MSRPWIEESWRLLLFVAAALLIGAFTGQSGWLLSISLLLYIGNL
                     LLRLRRLEQWVRHHDRDPPDLDGVFDEVIYHLHRHRKRQHARRSRIAQELRQFQRSAR
                     ILPDATVLLDAHDQILWFNRAATDLLGLRSSDTGHAISGLIRSPQFIRLLREHSSDSN
                     YDTLEMMSPVDERILLDLRISSGRNEQRLLLARDVTQLNRLLNMRRDFVANVSHELRT
                     PLTVVIGYLESLLDDDIQETDPATLKSLLGKIYAPAQRMKTLVEDLLLLSRLDTDTML
                     NAENCPLVEMPALLRSAARDCEQLSKGQHSFSYRIDPQLKLRGVEQELYSVASNLLGN
                     AIRYTPPGGKIELQWQVEPDGRARLSVTDNGPGIAPAHIERLTERFYRVDVGRSRQAG
                     GTGLGLSIVKQVIRRHGAELRIDSQPGHGSSFHCLFPVNRTLVQAARKLAPAAAHDAI
                     VTNAQQ"
     gene            2414..3385
                     /locus_tag="PKI23_08745"
     CDS             2414..3385
                     /locus_tag="PKI23_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HNN37063.1"
                     /translation="MKTLRLLSLVSVSAALASPWAVAETAVDPALPSYSKASGVSGNL
                     SSVGSDTLANLMTFWAEEFKRQYPNVNIQIQAAGSSTAPPALTEGTSNIGPMSREMKG
                     NEVAAFEDKYGYKPTAIRVAVDALAVFVHKDNPIASLTIPQVDAIFSTTRKCGGADDI
                     GTWGQLGLTGDWEKRSIQIYGRNSVSGTYGYFKEHALCKGDFKANVNEQPGSASVVQS
                     ISTSLNSIGYSGIGYTTPGVRALPLATKEGAKPVEATEANALNKSYPLSRYLYVYVNQ
                     EPDKALAPIEREFIKLILSKQGQQVVVKDGYVPLPASVAAEDLAKIK"
     gene            3550..5787
                     /locus_tag="PKI23_08750"
     CDS             3550..5787
                     /locus_tag="PKI23_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNN37064.1"
                     /translation="MNKAGFTLITSPRQRRRLLLDRSMEKVIAAGGLWVIVTILAIFI
                     YLLYVVLPIFQSASITPHGNFALPAAEQGESLLIDVEESFQLGSRLTANGQLSFFNLT
                     DGQLVRQEQLPLAAGQKIVKALAIGAVQRHLVLLLEGNQLLIAKVSYKVSFDGDRRVL
                     TPRVQYPHGKTPLALPASAPLIDLDAVLENEALILALVDDSRQLQLLSYRTEYGEPLT
                     TLEQQAVIPAPQGVAAVHIDANALWLYLVGQDNSIQLFDLRDRSAPLALHTFSDWVGG
                     SNLIGSQLLLGGSSLLVADAEQQIHQLSIQRDSENRYQLRQVRSFNSSGSISTLIPER
                     LRKGFTVLDQEGGLDLFYTTSERTLLREEKLLPAALRSAAMAPRGDGLLVETRDNRLL
                     AYELHNPHPEISWSTLWSKVQYEGYPEPQYIWQSSAANNDFEAKFSLIPLVFGTFKAA
                     FYAMLFSVPLAILGAIYTAYFMSPKMRGWVKPAIEMMAALPTVILGFLAGLWLAPMIE
                     NHLLAIFLLLLMLGPLFFLCAWLWHLAPAALRYRLPEGWEALLLVPVVLFGIWLAFAV
                     APGIESLLFGGSLRRWLPEVAGIHYDQRNALVVGIAMGVAVIPNIFSITEDAIFSVPK
                     HLTLGSLALGATPWQTLTRVVLLTASPGIFSAVMIGMGRAVGETMIVLMATGNTPVMD
                     FSIFEGLRTLSANIAVEMPESEVNSSHYRILFLAALVLFAVTFIFNTAAELVRQNLRK
                     RYGNL"
     gene            5774..7480
                     /gene="pstA"
                     /locus_tag="PKI23_08755"
     CDS             5774..7480
                     /gene="pstA"
                     /locus_tag="PKI23_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNN37065.1"
                     /translation="MEISDQPTRASVPRPSFNAWRNSGAPWIWLNAGAISISLIVVLT
                     LLGLLTVRGLSHFWPATLHEYRYQLSDGTQERIIGQQVNAEFISVNQYRESTGQAAPA
                     GAEQVERWLIKVGNRELLPADFRWLDHHLIKQIDSPVHLVRIERTEWGDMFGTLQGIT
                     EAGQPVATGSDASWLALQPRLERAKTLRAEIRALEKGEIGAINHQLERLRLERRKLAL
                     QNQQSDEALADIKTREDAQHAQFELLQQRLIELHEQLARDTLQIEVMTGQVVDVPLSR
                     VIEATRPNSLSLFSKLQCYAINLWNFLSDDPREANTEGGIFPAIFGTVMMVLLMSVMV
                     TPFGIVTAVYMREYARQGPLIRAIRIAVNNLAGVPSIVYGVFGLGFFVYLLGGQIDQW
                     FFAEALPAPTLGTPGILWASLTLALLTVPVVIVSTEEGLARIPRSIREGSLAVGATRF
                     ETLWRVVLPMASPAIMTGMILAIARAAGEVAPLMLVGVVKLAPTMPIDGNFPFFHLDR
                     KFMHLGFHIYDVGFQSPNVEAARPLVYATSFFLVLVIILLNLTVILLRNRLREKFSAL
                     DN"
     gene            7509..8330
                     /gene="pstB"
                     /locus_tag="PKI23_08760"
     CDS             7509..8330
                     /gene="pstB"
                     /locus_tag="PKI23_08760"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNN37066.1"
                     /translation="MGVTVDRPNRSIDTPRKLGIADESVCIKVEHLDLLYGEKQALFD
                     VNMQIPEHCVTAFIGPSGCGKSTLLRCFNRMNDLVDGVQVNGRILLQGQDIHDPSVNV
                     SDLRRHVGMVFQKPNPFPKSIYENVAYGLRLQGVKQRRVLDEKIEWALQSAALWDEVK
                     DRLDDNAMGLSGGQQQRLVIARAIAIEPSVILLDEPASALDPISTLKIEELIYELKSN
                     YTIVIVTHNMQQAARISDYTAFMYLGKLIEFDNTDTLFTKPAKKQTEDYITGRYG"
     gene            8348..9076
                     /gene="phoU"
                     /locus_tag="PKI23_08765"
     CDS             8348..9076
                     /gene="phoU"
                     /locus_tag="PKI23_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016394384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNN37067.1"
                     /translation="MERSHHISEQYNLELDKLRTQVLTMGGLVETQILDAITALVDGD
                     TQKAEQVIHGDIQVNSMEVTIDEQCTQIIARRQPTAVDLRLLIAILKTITDLERIGDE
                     AEKIAKMAYELSTMDRPINQYIEIKHLGGHVRKMLHDTLDAFARMDVEAALAVIAEDK
                     VVDREYEASMRQLITIMMEDQRSIRRVLNTLWSARALERIGDHAKNICEYVIFCVKGK
                     DVRHIGLEQKQRTALDAPTLGEAE"
     gene            complement(9121..9420)
                     /locus_tag="PKI23_08770"
     CDS             complement(9121..9420)
                     /locus_tag="PKI23_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HNN37068.1"
                     /translation="MEPATTIELQAAAFRHLLEHLRARPQLQNIELMNLADFCRNCLA
                     KWLVEAAAERGLSLDYEAAREQVYGMPYSEWKAHHQREATPEQLAAFEQRQNSKS"
     gene            9544..>10061
                     /locus_tag="PKI23_08775"
     CDS             9544..>10061
                     /locus_tag="PKI23_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37069.1"
                     /translation="MSEFPHAASASVPPLDGTPWSQAWQALIPHQQRLAGQRLSALFS
                     TDPRRAERYSVSAAGLQLDYAKNRLDDPALAALLTLAEAAGVSDGIAAMFRGEPINPT
                     EARPALHIALRGSFTDQADLAVNAEVDSTLARLEALVTSVANRDWRGFSDRPITDIVP
                     AFEGLVGLSIARG"
BASE COUNT         1924 a         3350 c         2955 g         1832 t
ORIGIN      
        1 tcttcatgca ctgcctgccg gcccaccgtg gcgaagaggt cagcgaggag atgctcgacg
       61 accaccgctc ggtggtgtgg gaggaggccg agaaccgcct gcatgcgcag aaggcgctga
      121 tggagtggct gctgcaacag cactgagcag catccgcacc gtcacactca ctgccccggc
      181 accgacaatt gttacaattg tcatccagcc gtcattcatg acggattaaa tgtttccttc
      241 atctccacct gcacgaggtt gatttgacat ggaaatacac gggcaaaaga ttctggtcgt
      301 cgaagatgag ccggagattc gtgacatgct ggccttcagc ctcgaacgtg gcggctatgc
      361 ggtcactttg gccgaagatg gcgaaagcgc gctgcgcctg cttgatcagc agcgtccgga
      421 tctgattctg atcgactgga tgttgccggg cctcagcggc atcgagctgg ccaaccggct
      481 gcggcgcgat gaacccaccc aggagctgcc gctgatcatg ctgaccgccc gcggcgagga
      541 gagcgacaag ctgaaatcct tctcggtcgg catcgatgac tacatcacca agcccttctc
      601 ctccaaggag ctgctggcgc gcatcaaggc gctgctgcgc cgcagtggca gaggtgacga
      661 ggggcgcctt gccatcgacg gactctgcct cgacacccag gggcaccggc tgctggtcga
      721 cggcaagccg gtccacatcg gcccgaccga atatcggctg ctggagctgt tcatgagcaa
      781 tcccgaccgg gtctacaacc gcgaccagct gctcgatcgg gtctggggcc gcacggtcta
      841 tgtcgaggag cgcacggtgg acgtccacat tctgcggctg cgcaagaccc tggcacccca
      901 tggctatgat cggctgatcc agaccgtgcg cggcaccggc tatcgcctct ccagaacctg
      961 agcatgtctc gcccctggat cgaggagtcg tggcggctgc tgctgttcgt tgccgcagcg
     1021 ctgctgatcg gcgccttcac cggccagagc ggctggctgc tgagcatcag cctgctgctc
     1081 tatatcggca acctgctgct gcgcctgcgt cggctcgaac agtgggtacg gcaccatgac
     1141 cgcgacccgc ccgacctcga tggcgtcttc gatgaggtca tctaccatct gcatcgccac
     1201 cgcaagcgtc aacatgcacg ccgcagccgc atcgcccagg agctgcggca gttccagcgc
     1261 tcggcgcgaa tcctgcccga tgccaccgtg ctgctcgatg cccatgacca gattctctgg
     1321 ttcaaccgcg ccgccactga tctgctggga ctgcgctcca gcgacaccgg ccatgccatc
     1381 agcggcctga ttcgcagtcc gcagttcatc cgcctgctgc gcgagcacag cagcgacagc
     1441 aactacgaca ccctggagat gatgtcaccg gtcgatgagc gcatcctgct cgatctgcgc
     1501 atctccagcg gccgcaacga gcagcgcctg ctgctggcgc gcgatgtcac ccagctcaac
     1561 cggctgctga acatgcggcg tgacttcgtc gccaatgtct cccacgagct gcgcacaccg
     1621 ctgacggtgg tgatcggcta cctcgaaagc ctgctcgatg acgacattca agagaccgac
     1681 cccgcgacac tgaagagcct gctcggcaag atctatgccc ctgcccagcg aatgaagacc
     1741 ctggtcgagg atctgctgct gctgtcgcgg ctcgacaccg acaccatgct caatgccgaa
     1801 aactgcccgc tggtcgagat gccggccctg cttcgttcgg ccgcacgcga ttgcgaacag
     1861 ctcagcaagg ggcaacacag cttcagctac cggatcgatc cccagctcaa gctgcgcggc
     1921 gtcgagcagg agctctacag cgtggccagc aacctgctgg gcaacgccat tcgctacacc
     1981 ccaccaggcg gaaagatcga gctgcaatgg caggtcgagc cagacggtcg ggcgcggctc
     2041 agtgtcaccg acaacggccc cggcatcgca ccggcccaca tcgagcggct caccgagcgc
     2101 ttctaccggg tcgatgtcgg tcgctcacgt caagccggcg gcaccggcct ggggctgtcg
     2161 atcgtcaagc aggtgatacg ccgccatggc gccgaactga gaatcgacag ccaaccggga
     2221 catggcagca gcttccactg tctgtttccg gtcaaccgga ccctggtgca agctgcgcga
     2281 aagctcgccc cggctgcggc gcacgatgcg attgtcacaa acgcacagca gtgatccgcc
     2341 ctttcgtaac ctgactgtca caatcgattc cgataatcgc tgccatccgc cactcatgga
     2401 gatcacaccc gcaatgaaaa ccctcagact tctttctctg gtcagcgtct cggctgccct
     2461 ggccagccct tgggctgtcg ccgaaaccgc cgtcgatccg gccctgccca gctacagcaa
     2521 ggccagcggt gtctccggca acctgtcgag cgtcggttcc gacacactgg ccaatctgat
     2581 gaccttctgg gccgaagagt tcaagcgcca atacccgaat gtcaacatcc agatccaggc
     2641 tgccggctcc tccaccgcgc caccagcgct gaccgaaggc acctccaaca tcggcccgat
     2701 gagccgggag atgaaaggca acgaggtcgc tgccttcgaa gacaaatatg gctacaaacc
     2761 caccgcgatc cgggtggcgg tcgatgcact ggcggtcttc gtccacaagg acaatccgat
     2821 tgcgtcactc accattccgc aggtggatgc catcttctcc accacccgca agtgcggtgg
     2881 tgccgatgac atcggcacct gggggcaact cggcctgacc ggcgactggg agaaacgctc
     2941 gatccagatc tacggccgca actcggtctc cggcacctac ggctacttca aggagcatgc
     3001 actgtgcaag ggcgacttca aggccaacgt caacgagcag ccaggctccg catcggtggt
     3061 gcagagcatc agcacctcgc tgaacagcat cggttactcc ggcatcggtt atacgacgcc
     3121 cggcgtgcgt gcactgccac tggccaccaa ggagggcgcc aagccggtcg aggccaccga
     3181 ggccaatgcg ctcaacaaga gctatccgct gtcgcgttac ctctatgtct atgtgaatca
     3241 ggagccggac aaggcgctgg caccgatcga gcgtgagttc atcaagctga tcctctccaa
     3301 gcagggccag caggtggtgg tcaaggatgg ctatgtcccg ctgcccgcca gcgtggctgc
     3361 tgaagacctg gccaagatca agtgatcggc ctgtgactgt gcatgaaggg agactgcccg
     3421 ccagtctccc ttcatgtttt ctgaatatcc tctgctgttt tctgaccggt ctggtatctt
     3481 tgcgcgccgg atcgacatga acggcaccat gccacacggc atcccttttc aattctggac
     3541 gactcgccga tgaacaaggc cggtttcacc ctgatcacct cgccgcgcca gcgccgccgc
     3601 ctgctgctcg acagatcgat ggaaaaggtg attgccgcag gcggcctgtg ggtgatcgtc
     3661 accatcctgg caatcttcat ctacctgctc tatgtcgtgc tgccgatctt ccagtcggct
     3721 tcgatcaccc cacacggcaa ctttgcgctg ccggcggccg aacagggtga atcactgctg
     3781 atcgatgtcg aagagagctt ccagctgggc agccgcctga ccgccaacgg ccagttgagc
     3841 ttcttcaatc tgaccgacgg ccaactggtc cgtcaggagc agctgccgct ggctgccgga
     3901 cagaagatcg tcaaggccct ggcgattggc gccgtacaac gccatctggt gctgctgctc
     3961 gaaggcaacc agctgctgat cgccaaggtg agctacaagg tcagcttcga cggcgatcgg
     4021 cgcgtcctga cgcctcgggt gcagtacccc catggcaaga cgcccctggc cctgcccgcc
     4081 agcgcacccc tcattgatct ggacgccgtg ctggaaaacg aagccctgat tctggcgctg
     4141 gtcgatgaca gcaggcagtt gcagttgttg agctatcgga ccgagtatgg cgaaccgctg
     4201 accactctcg aacaacaggc agtgattcca gcaccacagg gcgtggctgc ggtgcacatc
     4261 gacgccaatg cgctctggct ctacctggtc ggtcaggaca acagcatcca gctgttcgac
     4321 ctgcgcgacc gcagcgcacc gctggcactg cacaccttca gcgactgggt gggcggcagc
     4381 aatctgatcg gcagccagtt gctgctgggc ggttcatcac tgctggtcgc cgatgccgag
     4441 cagcagatcc atcaactgtc gatccagcgc gacagcgaaa accgctatca actgcgtcag
     4501 gtccgcagct tcaacagcag cggctccatc tcgaccctga ttccggaacg gttgcgcaag
     4561 ggcttcaccg tgcttgacca ggagggtggc ctcgacctct tctacaccac ctcggagcgc
     4621 actttgctgc gcgaagagaa gctgctgccg gccgccctgc gcagcgccgc catggcaccg
     4681 cgcggcgatg gcctgctggt cgaaacccgc gacaaccggc tgctcgctta tgagctgcac
     4741 aatccgcatc cggagatctc ctggagcacg ctctggtcga aggtgcagta cgaaggctac
     4801 ccggaacccc agtacatctg gcaatcctcc gctgccaaca acgatttcga ggccaaattc
     4861 agcctgatac cgctggtctt cggcaccttc aaggcagcct tctatgcgat gctgttctcg
     4921 gtgccgctgg ccattctcgg cgccatctac accgcctact tcatgtcgcc gaagatgcgt
     4981 ggctgggtca agccggccat tgaaatgatg gcggcgctgc cgacggtgat tcttggcttt
     5041 ctggccgggc tctggctggc gccgatgatc gagaaccacc tgctggcgat ctttctgctg
     5101 ctgctgatgc tggggccgct gttctttctc tgcgcctggc tctggcacct cgctcccgct
     5161 gcattgcgct accgtctgcc cgaaggctgg gaagccctgc tgctggtgcc ggtggtgctc
     5221 ttcggcatct ggctcgcctt tgcggtggcc cccggaatcg agagcctgct gttcggcggc
     5281 agcctgcgcc gctggttgcc ggaggtggca ggcatccact acgaccagcg caacgcgctg
     5341 gtggtgggca ttgcgatggg cgtggcggtg attcccaaca tcttttcgat caccgaggat
     5401 gccatcttca gcgtgcccaa gcacctgact ctcggctcgc tggcactcgg tgccaccccc
     5461 tggcagaccc tgacccgggt ggtgctgctg accgccagcc ccggcatctt ttcggcggtc
     5521 atgatcggca tgggccgggc cgtcggcgaa accatgatcg tgctgatggc caccggcaac
     5581 acgccggtga tggatttcag catcttcgaa gggctgcgca ccctctcggc caacatcgcg
     5641 gtggagatgc cggaatcgga ggtgaacagc agccactacc ggattctgtt cctggcggca
     5701 ctggtgctct ttgccgtcac cttcatcttc aacaccgcgg ccgaactggt ccggcagaat
     5761 ctgcggaagc gttatggaaa tctctgatca accgaccagg gcgagtgtgc ctcgcccttc
     5821 gttcaacgcc tggcgcaaca gcggcgcccc ctggatctgg ctcaatgccg gcgccatcag
     5881 catcagcctg atcgtggtac tgaccctgct gggattgctc accgtgcgcg gtctctccca
     5941 cttctggcca gccaccctcc atgagtaccg ctatcaactg tccgatggca cgcaggagcg
     6001 catcatcggc cagcaggtca atgccgagtt catctccgtc aaccagtacc gcgaatcgac
     6061 cggtcaggcc gcgccggccg gcgccgaaca ggtcgagcgc tggctgatca aggtgggcaa
     6121 ccgcgagctg ctgccagccg attttcgctg gctcgaccac catctgatca aacagatcga
     6181 cagccccgtc cacctggtgc gcatcgaacg caccgaatgg ggtgacatgt tcggcacgct
     6241 gcaaggcatc accgaggccg gacagccggt cgccaccggc agtgacgcca gctggctggc
     6301 gctgcaaccc cggctcgagc gggccaaaac cctgcgagcc gagatccgcg cgctggagaa
     6361 gggcgaaatc ggcgcgatca accaccaact ggagcggctg cggctcgagc ggcgcaaact
     6421 ggcactgcaa aatcagcaga gcgatgaagc gcttgccgac atcaagaccc gggaggatgc
     6481 ccagcatgcc cagttcgaac tgctgcaaca gcgcctgatc gaactgcatg agcaactggc
     6541 acgcgacacg ctgcaaatcg aggtgatgac cgggcaggtc gtcgatgtcc cgctgtcgcg
     6601 ggtgatcgag gccacccgac ccaacagcct gtcgctcttc tcgaagctgc agtgctatgc
     6661 catcaacctg tggaatttcc tcagcgacga tccacgggag gccaacaccg agggcggcat
     6721 ctttccggcc attttcggca ccgtgatgat ggtgctgctg atgtcggtga tggtcacccc
     6781 tttcggcatc gtcaccgccg tctacatgcg tgaatatgcg cgccagggcc cactgatccg
     6841 cgccatccgc atcgccgtca acaacctcgc cggtgtgccg tcgatcgtct atggggtgtt
     6901 tggcctgggc ttcttcgtct acctgctcgg tgggcaaatc gaccagtggt tctttgccga
     6961 agccctgccg gccccgactt tgggcacgcc cggtatcctg tgggcatcac tgaccttggc
     7021 cctgctcacg gtgccggtgg tgatcgtctc gaccgaagag ggcttggcgc gcatcccgcg
     7081 ttcgatccgc gaaggcagcc tggcagtggg tgccacccgc ttcgagaccc tgtggcgggt
     7141 ggtgctgccg atggccagcc cagccatcat gaccggcatg atcctcgcca tcgcccgcgc
     7201 cgctggtgaa gtggcgccgc tgatgctggt cggcgtggtc aagctggcgc cgaccatgcc
     7261 gatcgacggc aacttcccgt tcttccatct ggaccgcaag ttcatgcacc tggggtttca
     7321 catctatgat gtcggcttcc agagccccaa tgtcgaggcg gcccggccgc tggtctatgc
     7381 gacctcgttc tttctggtac tggtcatcat cctgctcaac ctgaccgtga tccttctacg
     7441 caaccggcta cgggagaaat tcagtgccct cgacaactga agccagttca tacatcgtcc
     7501 ccaacggcat gggtgtcacc gtggatcgac ccaatcgcag catcgatacc ccccgcaagc
     7561 ttggcattgc cgatgagtcg gtctgcatca aggtggagca cctcgacctg ctctatggcg
     7621 aaaaacaggc gctgttcgac gtcaacatgc agattccaga acactgcgtc actgccttca
     7681 tcggtcccag cggttgcggc aaatcaaccc tgttgcgctg cttcaaccgc atgaacgacc
     7741 tggtcgatgg cgtacaggtg aatggccgga tcctgctgca ggggcaggac attcacgacc
     7801 cgagcgtcaa tgtctccgac ctgcgccgcc atgtcggcat ggtgtttcag aagcccaacc
     7861 cctttccaaa atcgatctac gagaatgtcg cctacggcct gcggctgcag ggggtgaaac
     7921 agcggcgtgt gctcgacgag aagatcgagt gggccctgca gagcgcggca ctgtgggatg
     7981 aggtcaagga ccggctcgac gacaacgcca tggggctctc cggcggccag cagcagcggc
     8041 tggtgatcgc gcgcgccatc gccatcgagc cctcggtgat cctgctcgat gagccggcct
     8101 cggcactcga cccgatctcg acgctgaaga tcgaggagct gatctacgag ctcaagtcga
     8161 actacaccat cgtcatcgtc acccacaaca tgcagcaggc ggcccgcatc tccgactaca
     8221 ctgccttcat gtacctgggc aaactgatcg agttcgacaa caccgacacc ctcttcacca
     8281 agccagccaa gaagcagacc gaggattaca tcaccggccg ctacggctga ggccacagga
     8341 gtcagcaatg gaacgatcac accacatcag cgagcagtac aacctggagc tcgacaagct
     8401 ccgcacccag gtgctcacca tgggcggtct ggtggagacc cagattctgg atgccatcac
     8461 ggcactggtg gatggcgaca cccagaaggc cgaacaggtc attcacggcg acatccaggt
     8521 caactcgatg gaggtgacga tcgatgagca gtgcacccag atcatcgccc ggcgtcagcc
     8581 caccgcggtg gacctgcgcc tgctgatcgc catcctgaag accatcaccg acctcgaacg
     8641 catcggtgac gaggccgaaa agatcgccaa gatggcctat gagctgtcga cgatggaccg
     8701 gccgatcaac cagtacatcg agatcaagca tctgggcggc catgtccgaa agatgctgca
     8761 tgacaccctg gatgccttcg cccgcatgga tgtcgaggcg gccctcgccg tcatcgccga
     8821 agacaaggtg gtcgaccgcg aatatgaggc gtccatgcgc cagttgatca ccatcatgat
     8881 ggaggatcag cgcagcatcc ggcgggtgct caacaccctc tggtcggccc gcgcgctgga
     8941 gcgcatcggc gaccatgcca agaacatctg cgaatatgtg atcttctgcg tcaagggcaa
     9001 ggatgtccgc cacatcggtc tggagcagaa gcagcggacc gcactggatg cacccaccct
     9061 cggggaggcg gagtagcacc gcaacgaagc gtcgcgggtc agccaccgac ccgcctccgc
     9121 tcaactcttg ctgttctgcc tctgctcgaa ggcggccaac tgctcgggtg tggcctcgcg
     9181 ctggtgatgt gccttccact cgctgtaggg catgccatag acctgctccc gcgccgcttc
     9241 gtaatcgagc gacaggccac gttcggccgc tgcctccacc agccacttgg ccaggcagtt
     9301 gcggcagaaa tcagccaggt tcatcaactc aatgttctgc agttgcggcc gtgcccgcag
     9361 atgctcgagc agatggcgaa aagccgcggc ttgcagttca atggtcgttg cgggctccat
     9421 cattcacctc cagcgattca gttgaaagcg tgcacaccgc acgcgccaga tggaccatgg
     9481 tagcaagaat tggcccgatc ctggcttcaa aaacccattc acggtcgcaa gacgagaacc
     9541 accatgtcag aatttcccca cgccgcatcc gcttcggtac cgccgctgga cggcactccc
     9601 tggagccagg cctggcaggc gttgattccc catcagcagc ggctggccgg gcagaggctt
     9661 tctgcgctgt tcagtaccga tccccggcgt gccgaacgct acagcgtgag cgccgccgga
     9721 ctgcaactcg actacgccaa gaaccggctc gatgacccag cgcttgccgc cctgctgaca
     9781 ctggccgaag cggccggcgt cagcgacggg atcgccgcca tgttccgggg tgaaccgatc
     9841 aatccgaccg aagcgcgtcc tgccctgcac atcgccctgc gtggcagctt caccgaccaa
     9901 gccgatctgg ccgtcaatgc cgaggtcgac agcaccttgg cccggctgga ggcgctggtc
     9961 acctcggtgg cgaaccgcga ttggcgcggc ttcagcgacc gaccgatcac cgacatcgtc
    10021 cccgcgttcg aggggctcgt cggcctgagc atcgcccgtg g
//
LOCUS       DAORCQ010000081        10018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4615_length_10018_cov_5.811603, whole genome shotgun sequence.
ACCESSION   DAORCQ010000081 DAORCQ010000000
VERSION     DAORCQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4615_length_10018_cov_5.811603"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /gene="prsT"
                     /locus_tag="PKI23_08780"
     CDS             <1..1879
                     /gene="prsT"
                     /locus_tag="PKI23_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNN37070.1"
                     /translation="GDYLPAWRQLALLQLQKKSYPEAELSLRKVLGFQPDDLAFGKLL
                     AEVLMRQFKLNEASALLDSLAKNHSGDPGVIALRSSLALKQGDSEAAQTQLNQLSSAS
                     KSPLQSHWQEGMINLANGQFDAAIKSLRQAQSEEPDQPLLQLALAQAHLGKRDVDAAL
                     PLAQSLQKKFPEQARIQALIGAILATKGDYNSAQAAFAKALELEPGDPDSSHQLAKLA
                     LINKDPQGARRYYEAALARHPQSWITAVRLAGLSELEGHPDDAEQRLRKAVSDYPLEI
                     PVHQALALSLFRHGKTDEALTHLSDLTLKHGNDFDALTALAELQRLAGRHVNAAETYR
                     TLAEKYPSPNSYYQLADAELRNQNYHQARQALAQAEKMSGAHPQLTVSRIRIALLEGD
                     LAAAEKLLGELVGQSKGENGLTHRLRGEIALARKQPLEAAHAFEKSLAVEPNPDLAIA
                     LIQNLNRAGKGDEARSATAKWLKRFPDHAGLRVVHAEQLMLQGADEQAIAVYQGLLKS
                     TPQSPAVLNNLAYLHIKRDPKAAVSYAEQAAKLAPGSAEIADTHGWALLHAGAPEQAL
                     PILQQALQKTPNNPSIGYHLAAVQQKLGDSAAAKQLLSGLIEQKFPEQAEAQALYQKL
                     P"
     gene            1927..3114
                     /locus_tag="PKI23_08785"
     CDS             1927..3114
                     /locus_tag="PKI23_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010656629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37071.1"
                     /translation="METLTQFRSETRAWLEANCPPSMRTPMGEAEIVWGGRNARFSHP
                     DARLWLERMAQKGWTAPTWPLDYGGGGLSGEQNAILQQELRRLQCRPALQSFGLWMLG
                     PVLLEFANEAQKQTHLPPIVRGEIRWCQGYSEPGAGSDLAGLQTRCEEQDDHYLVNGQ
                     KIWTSYADQADWCFCLVRTDPRAAKHNGISFILIDMASPGVSTSPIRLISGASPFCQT
                     FFDNVRVPKENLIGEPNKGWTIAKRLLQHERSMLSGMGGMGGGGRRSLPKLAKQAVGE
                     VDGRIADPLLRDHITRHRMDSRAFALTLQRSAEEQKAGQSVGAVSSMFKYYGTELNKR
                     NYELLIESLGTQALGWSGPGFSDDELLIPREWLRSKGNSIEGGTSEVQLNVIAKQVLG
                     LPD"
     gene            3143..4282
                     /locus_tag="PKI23_08790"
     CDS             3143..4282
                     /locus_tag="PKI23_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37072.1"
                     /translation="MALALNDEQRFLKQTAAEFFAQQAPIAQLRQLRDQHSADGFDRA
                     TWQEMVELGWSGILIPERFGGSDFGFLGLALVLEESGRTLAATPLLATALIGASALLL
                     GGSAAQQQAHLPAIAAGQRLSALAIEEQGRHAPYQIACRVERQGSGFVLDGSKQFVID
                     GHVADLLIVSARSRGQPGERDGITLLLVPADAPGVTITRNQMVDSRNAASIRFDRVKL
                     EADALLGQEGEGAALLDALLDRARIGLAAEMLGSLQECFERTIGYLKERQQFGVAIGS
                     FQALKHRAAQMFCEVELCKSVVLQAASSIDDAPDQSALLASLAKARLCDTATLVSNEG
                     IQMHGGIGMTDEFDIGFFIKRARVAAHAFGDANYHRDRYATLDGY"
     gene            4540..4746
                     /locus_tag="PKI23_08795"
     CDS             4540..4746
                     /locus_tag="PKI23_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HNN37073.1"
                     /translation="MSKKVKRDVEQRAFTRGYQAGINGKSKELCPHTATTGRQSWLDG
                     WREGRGDLWGGMQGVSAIHRLPTI"
     gene            complement(4862..5917)
                     /locus_tag="PKI23_08800"
     CDS             complement(4862..5917)
                     /locus_tag="PKI23_08800"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003175505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNN37074.1"
                     /translation="MTGTKMLYSLLRPLLFMLEPERAHHWALEGLAWSERLRLAGPLF
                     GGLSVPPPLSTQVMGLSFPNPLGLAAGLDKNGDYIDALATLGFGFIEVGTVTPRPQIG
                     NPQPRLFRLPARRALINRMGFNNLGVDHLAARLAASGHRGIVGVNIGKNADTALERAV
                     DDYLHGMNRLYPLASYITVNLSSPNTPGLRSLQEGAAFSALLERLKSAQAALATEHRR
                     YVPLVIKIAPDFDRGAVEALGAQLLQFEVDGVIATNTTVSRQGVEGEVLAKEAGGLSG
                     RPLLHPTNEVLRWLAEVLEARIPIIGCGGVEDAWSFEQKRTAGAQLVQLYTALIYQGP
                     WLVRRMLAELALHQQRA"
     gene            6080..6904
                     /locus_tag="PKI23_08805"
     CDS             6080..6904
                     /locus_tag="PKI23_08805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNN37075.1"
                     /translation="MFKNRIRGLLCAIVLLSVQGQALAARDIFSGSADAYLVKVGNRT
                     LWDESQDVRLPPASLTKMMTALLALERVEMSKRVTISRAAAAETGSKIGVRVGETYSV
                     HSMLAAALIRSGNDACHALADAVSGSEQRFVALMNSRAKEMGLRNTQFTNACGHDDPA
                     HYSSAHDLAILGERLLNHKIAADLVSKKNFTIRALDGKKRSITFKNSNHLLGEYPGML
                     GGKTGYTPGAGKCLVAVAKRGNSRALIVLLNAPNRWQTATQMLNKALDQRTTTAMR"
     gene            complement(7112..7447)
                     /locus_tag="PKI23_08810"
     CDS             complement(7112..7447)
                     /locus_tag="PKI23_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNN37076.1"
                     /translation="MNTMTINGYQAVIAFDPDIQMFRGEFVGLNGGADFYAKDVDGLR
                     REGEISLRIFLEACAEDGVDPRKYFSGKFSLRIDPELHEAAAIAAAAHGQSLNQWAAE
                     AIRRAARAQ"
     gene            complement(7444..7752)
                     /locus_tag="PKI23_08815"
     CDS             complement(7444..7752)
                     /locus_tag="PKI23_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNN37077.1"
                     /translation="MACSIALLVDTIICYHSSMKRKHQRTLEQIFARPVSANVRWTDI
                     EVLFVELGGQVTEREGSRVLVRLFGDRRVFHRPHPEPTTDKGAVEAIRKWLEQHGVKP
                     "
     gene            complement(7847..8935)
                     /gene="ychF"
                     /locus_tag="PKI23_08820"
     CDS             complement(7847..8935)
                     /gene="ychF"
                     /locus_tag="PKI23_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNN37078.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQAGIDAANFPFCTIDPHTGVV
                     PMPDPRLDRLAQIVQPERVVPTTMEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAQ
                     VVRCFEDDNIIHVANRVDPVSDIEVINTELALADLDTVEKNLARSARAAKGGDKEAIA
                     LHALLERIQPELNQARPVRTLGLEPAALALLRPLHLLTVKPTLYIANVAEGEEDNDHV
                     RAVRAVAAAEGAEVVVICNQLEAEIAELEESEKAEFLESLGFEEPGLNRLIRAGYRLL
                     GLHTYFTAGQKEVRAWTIPIGATAPKAAAVIHTDFERGFIRAEVIAYDDFVAGNGEQG
                     AKEAGKWRLEGKEYIVADGDVIHFRFNV"
     gene            complement(8954..9526)
                     /gene="pth"
                     /locus_tag="PKI23_08825"
     CDS             complement(8954..9526)
                     /gene="pth"
                     /locus_tag="PKI23_08825"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNN37079.1"
                     /translation="MDAIVGLGNPGPDYAATRHNAGCWLVDRLAALAGVRLREERKFH
                     GRHARAFWQGCEFHLLQPDTFMNRSGRAVAALLRFYQIPPSRLLLVHDELDLPPGVAR
                     FKQGGGHGGHNGLRDIIEQLGGDKSFYRLRLGIGHPGDSSRVVTYVLNPPTAAEREAI
                     DQAMDDALRALPDALNGNWATAMSRLHAPT"
     gene            complement(9560..>10018)
                     /locus_tag="PKI23_08830"
     CDS             complement(9560..>10018)
                     /locus_tag="PKI23_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003099279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNN37080.1"
                     /translation="LAKSLESEAFYTHIINLKVGGSSEPVLLKALQRHPSKGSVMHAD
                     FMRVEMNRKIHVHVPLHFINEENCIGIKRDGGRIAHTLTEVEISCLPGDLPEYLEVDM
                     LDVGVGRHIHLSDLQLPPGVELVALSQGGDHDAQVVAVVPTKGGGDEAGG"
BASE COUNT         1927 a         3377 c         3058 g         1656 t
ORIGIN      
        1 gggcgactac ctgccggcct ggcgccagct cgcactgctg caactgcaga aaaaaagcta
       61 tccggaagcc gagctctcgc tgcgcaaggt gctcggcttt caaccggatg atctggcctt
      121 cggcaagctg ctggccgaag tgctgatgcg gcagttcaag ctgaacgaag ccagcgcact
      181 gctcgacagc cttgccaaaa accactccgg cgaccccggc gtgatcgcgt tgcgcagcag
      241 cctggcactc aagcagggtg acagtgaggc cgcccagacc caactcaacc agctcagcag
      301 tgccagcaaa tccccgctgc aatcgcactg gcaggagggc atgatcaacc tggccaacgg
      361 gcagttcgat gccgccatca aatcgctgcg gcaggcccag agcgaagagc ccgatcaacc
      421 gctgctgcag ttggcgctgg cccaggccca tctcggcaaa cgcgatgtcg atgccgcact
      481 gccgcttgcc caatcgctgc agaagaaatt tcccgagcag gcccgcatcc aggcactgat
      541 cggcgccatc ctggccacga agggggatta caactccgcc caggcggcct ttgccaaggc
      601 gttggagctc gaaccgggcg accctgacag cagccatcaa ctggccaagc tggccctgat
      661 caacaaggat ccgcaaggtg cccgccgtta ctacgaagcg gcgctcgcca ggcatccaca
      721 gagctggatc accgcggtgc gcctggccgg tctcagtgaa ctcgaaggcc atccggacga
      781 cgccgagcaa cggctgcgca aggcggtgag cgattacccg ctggagatcc cggttcacca
      841 ggcgctggcg ctctccctgt ttcgccacgg caagaccgac gaagcactca cccacctctc
      901 cgacctgacg ctgaagcacg gcaacgactt cgatgcgctg accgcgctgg ccgaactgca
      961 acggctggcc gggcgtcatg tcaacgcggc cgaaacctac cgcacgctgg ccgaaaagta
     1021 cccgagcccc aacagttact accagttggc cgatgccgaa ctgcgcaacc agaactacca
     1081 ccaggccagg caggcgctgg cacaggccga aaagatgagc ggagcacatc cgcaactgac
     1141 cgtttccagg atcagaatcg ctctgctcga gggtgatctc gccgctgccg aaaaactgct
     1201 tggcgagttg gtcggacaga gcaaaggcga aaatggcctg acccaccgct tgcgcggcga
     1261 gatcgccctg gcacgcaagc agccgctgga ggcggcccat gccttcgaaa aatcgctggc
     1321 cgtcgagccc aatcccgatc tcgccatcgc gctgattcaa aatctcaatc gggccggcaa
     1381 gggcgacgaa gcccgcagcg ccacggcaaa atggctgaag cgcttccccg accacgctgg
     1441 tctgcgcgtg gttcatgccg agcagctgat gctgcaaggc gccgatgagc aggccatcgc
     1501 cgtctaccag ggcctgctca agagcacgcc acagagcccg gcggtgctga acaacctcgc
     1561 ctacctgcat atcaagcgcg atccaaaggc ggcggtcagt tatgccgaac aggcggcaaa
     1621 gctggccccc ggctcggctg aaatcgccga cacccacggc tgggccctgc tgcatgccgg
     1681 cgcgccggag caggcgctgc caattcttca gcaagcgctg caaaagacgc cgaacaaccc
     1741 atcgatcggc taccatctgg ctgccgtcca gcagaaactg ggcgattcag ccgcagccaa
     1801 acagctgttg agcggcctca ttgagcagaa attcccagag caggccgaag cccaggcgct
     1861 ctaccagaaa ctgccctgat tcgacccgca acgcagacca tcatgcacgc aacaacggag
     1921 ggaaccgtgg agacgctgac ccaattccgc agtgaaaccc gcgcctggct ggaggccaac
     1981 tgcccaccca gcatgcgcac gccgatgggc gaagcggaga tcgtctgggg tggccgcaac
     2041 gcccggttca gccaccccga tgccagactc tggctcgagc gcatggcgca gaagggctgg
     2101 accgcgccga cctggccact cgactatggc ggtggcggtc tcagcggcga gcagaatgcc
     2161 atcctgcaac aggagttgcg ccgattgcag tgtcgaccgg ccctgcaatc cttcggtctg
     2221 tggatgctgg gcccggtgct gctcgaattc gccaacgagg cgcagaaaca gacccatctg
     2281 ccaccgatcg tgcgcggcga gattcgctgg tgccaaggct acagcgaacc cggggccggc
     2341 tccgatctgg ccggactcca gacccgctgc gaagagcagg acgaccacta tctggtcaat
     2401 ggccagaaga tctggacctc ctacgccgat caggccgact ggtgtttctg cctggtgcgc
     2461 accgacccca gggcggcgaa gcacaacggc atcagcttca tcctgatcga catggccagc
     2521 cccggcgtca gcacctcacc catcaggctg atcagcggcg cctctccctt ctgtcagacc
     2581 ttcttcgaca acgtccgggt gcccaaggag aatctgatcg gcgagcccaa caagggctgg
     2641 accatcgcca agcggctgct gcagcatgag cgctcgatgc tctccggcat gggcggcatg
     2701 ggcggcggcg gacggcgctc gctgccgaag ctggccaagc aggcggtcgg cgaggtcgat
     2761 ggccgcatcg ccgacccgct gctgcgcgat cacatcaccc gccaccgcat ggattcccgc
     2821 gccttcgcgc tgacactgca acgctcggcc gaagagcaga aagccggcca gagcgtcggg
     2881 gcagtctcct cgatgttcaa gtactacggc accgagctga acaagcgcaa ctacgagctg
     2941 ctgatcgaga gcctcggcac ccaggcgctc ggctggagcg gtccgggctt cagcgatgat
     3001 gaactgctga ttccacgtga atggctgcgc tcgaagggca actcgatcga aggcggcacc
     3061 tccgaggtcc agctcaacgt catcgccaag caggtgcttg gcttgcccga ctgaactcag
     3121 ggaacgaacc aaggagcaga ccatggcact ggcactcaac gacgagcagc gctttctcaa
     3181 acagaccgcc gcggagttct tcgcccaaca ggcaccgatc gcccagttgc gccagctgcg
     3241 cgaccagcac agcgccgacg gcttcgaccg cgccacctgg caggagatgg tcgaactggg
     3301 ctggtccggc atcctgattc cggaacggtt cggcggcagc gacttcggtt ttctcggcct
     3361 tgccctggtg ctggaagagt ccggtcgcac cctggccgcc accccgctgc tggccactgc
     3421 actgatcggc gcttcggccc tgctgctggg cggctctgcc gcgcagcagc aggcgcatct
     3481 gccagccatc gccgccggcc agcggctcag cgcgctcgcc atcgaggagc aggggcgcca
     3541 tgccccgtac cagatcgcct gccgtgtcga acgacagggc agcggcttcg ttctcgatgg
     3601 cagcaagcag ttcgtcatcg atggccatgt ggccgatctg ctgatcgtct cggcccgcag
     3661 ccgcggccag cccggcgagc gcgacggcat cacgctgctg ctggtccctg ccgatgcgcc
     3721 cggcgtcacc atcacccgca accagatggt cgacagccgc aacgccgcca gcatccgctt
     3781 cgaccgggtg aagctggagg ccgatgcgct gctcggtcag gagggtgaag gcgccgcgct
     3841 gctcgatgcc ctcctcgacc gcgcccgcat cggtctggcc gccgagatgc tcggcagcct
     3901 gcaagagtgt ttcgagcgca ccatcggcta cctcaaggag cgccagcagt tcggcgtcgc
     3961 catcggctca ttccaggccc tgaagcaccg tgctgcacag atgttctgcg aagtggaact
     4021 gtgcaagtcg gtggtgctgc aagccgccag cagcatcgac gacgcccccg accagagcgc
     4081 cctgctggcc agcctggcca aggccagact gtgcgacacc gccacgttgg tgagcaacga
     4141 gggcatccag atgcatggcg gcatcggcat gaccgatgag ttcgacatcg gctttttcat
     4201 caagcgggca cgcgtcgccg cccatgcctt tggcgatgca aactaccacc gcgaccgcta
     4261 cgccacgctc gatggctact gaatcggctg ggcgactgcc gcaccgcacg acagtctgca
     4321 gtctcgccct ccgccctcac caccgcgacc gatgaaacgt gtctaaaaag cgtggccgat
     4381 agcacaaaat cgttgtcgac acccgcgctt ggttacgctt agattacagg tgccggcagc
     4441 aattcacgga ggtgtcgtcc tgctgctttt cggccttcgg caccccgccg aagcgttctc
     4501 gttcgaagtc aaaaaaccaa acgatcgagg gtatttcgca tgagcaagaa agtgaaaaga
     4561 gatgtcgaac agcgcgcatt cacccgcggc tatcaggcag gcatcaacgg caagtcgaag
     4621 gagctctgtc cccacaccgc cacgaccgga cgacagtcct ggctcgacgg gtggcgcgag
     4681 gggcgcggag atttgtgggg cggcatgcag ggggtttcag ccattcaccg tctgcccacc
     4741 atctgatctg cggccatcca ccacacccaa gggtagacac ccatcaactg ccggcctcga
     4801 cggtcacgat cgacgccggt gatccagcag caaggcggcg ggcaacaccc gccgcctttt
     4861 ttcatgcccg ctgctgatgc agcgccaact cggcaagcat tcgtcgcacc agccaggggc
     4921 cctgatagat cagcgcggta tagagttgca ccagctgcgc gccggcggtg cgcttctgct
     4981 cgaagctcca cgcatcctcg accccgccac agccgatgat cggaatgcgc gcctccagca
     5041 cctcggccag ccagcgcagc acttcgttgg tcgggtgcag cagcgggcgg ccgctcaacc
     5101 cgcctgcctc tttcgccagc acctcacctt ccacgccctg cctcgacacc gtggtgttgg
     5161 tggcgatcac gccatcgacc tcgaactgca aaagttgcgc gcccagcgcc tcgaccgcac
     5221 cccggtcgaa atcgggcgcg atcttgatca ccagcggtac atagcggcgg tgctcggtcg
     5281 ccagcgccgc ctgtgccgat ttcagccgct ccagcagcgc gctgaaggcg gccccctcct
     5341 gcaaactgcg cagccccggc gtgttgggcg aggagagatt gacggtgatg taggaggcca
     5401 gcggatagag ccggttcatg ccatggagat agtcatcgac ggcccgctcc agtgccgtgt
     5461 cggcattctt gccgatgttg acgccgacga tgccgcgatg gccgctcgcg gccagccgtg
     5521 cagccagatg gtccaccccc aggttgttga aacccatgcg gttgatcagc gcccgcctgg
     5581 ccggcaaccg gaacagccgc ggctgcgggt tgccgatctg cggtcgcggc gtcaccgtgc
     5641 ccacctcgat gaaaccgaag ccgagtgtcg ccagcgcatc gatgtagtcg ccattcttgt
     5701 cgagcccggc cgccagcccc agcggattgg gaaaactcag ccccatcacc tgggtcgaga
     5761 gcggaggcgg caccgacaga ccaccgaaca acgggccggc aagccgcagc cgctcgctcc
     5821 atgccaaccc ctccagtgcc cagtggtgcg cgcgctcggg ctcaagcatg aacaacaaag
     5881 gtcgcaacag cgaatagagc attttcgttc cagtcatcga gagattttca tgatcacccc
     5941 actggcacag cgggcgattt taatttatct ttcaaggcag acggttacag ggcaatccag
     6001 aataaaaaca tcgagtttta tcggcctgaa agcaccgtct caaccccgtc cacacccacc
     6061 tgaattgcga aggcggcaca tgttcaaaaa cagaattcgc ggactgctgt gtgccattgt
     6121 actgctttcg gttcagggtc aggccctggc ggctcgggac atcttctccg gctcggccga
     6181 tgcctacctg gtcaaggtgg gcaaccgcac cttgtgggat gagtcgcagg atgttcggct
     6241 gccaccggcc agcctgacca agatgatgac cgcgctgctg gcgctcgagc gggtcgagat
     6301 gagcaagcgg gtcaccatct ccagggcggc ggcggccgaa accggcagca agatcggcgt
     6361 acgggtcggc gagacctaca gcgtgcactc gatgctggct gcggcgctga tccgctccgg
     6421 caatgacgcc tgccacgcac tggccgacgc cgtcagcggc agcgaacagc gctttgtcgc
     6481 cctgatgaac agccgcgcga aggagatggg cctgcgcaac acccagttca ccaatgcctg
     6541 cggccatgac gacccagccc actactccag cgcccatgac ctggcgatcc tgggcgagcg
     6601 gttgctcaac cacaagatcg ctgccgatct ggtcagcaaa aagaacttca ccatccgcgc
     6661 gctggatggc aaaaagcgca gcatcacctt caagaacagc aaccacctgc tcggcgagta
     6721 ccccggcatg ctgggcggca agaccggcta cacccccggt gccggaaaat gcctggtggc
     6781 cgtggccaag cgtggcaaca gccgcgcgct gatcgtgctg ctgaatgccc ccaaccgctg
     6841 gcaaaccgcc acccagatgc tgaacaaggc gctggatcag cgcaccacca ccgcgatgcg
     6901 ctgagcgccg gttgcggcaa cccactgatc gaggcgctgt ccacagcttt ggatcgacgc
     6961 gtgggagagc ttgcgccgct tcacacgacc gcgaaaatcc gtcagacgtt ggatgcgaag
     7021 tgcgacctga aagtcgcatc acttgagatg cagtgaaagg tcgctctttc gtacccggtt
     7081 ccatgcccgg tttcggcaat gccaccgctc atcactgcgc ccgtgccgct cgccggatcg
     7141 cctcggcggc ccactggttg aggctctgtc catgcgcggc agcagcgatg gcggcggctt
     7201 cgtggagttc gggatcgatg cgcagggaga actttccgga aaagtacttg cgcggatcga
     7261 cgccatcctc cgcgcaggct tcgaggaata tgcgcagcga gatttcgccc tcgcggcgca
     7321 ggccatcgac atccttggcg tagaagtcgg caccgccatt cagcccgacg aattctcccc
     7381 ggaacatctg gatgtccggg tcgaacgcga tgaccgcctg gtaaccattg atcgtcatgg
     7441 tgttcatggt ttcactccgt gttgttccag ccacttgcgg atggcctcga ctgccccctt
     7501 gtccgtggtc ggttcggggt gcggccgatg aaacacccgc ctgtcaccaa acaggcgcac
     7561 cagcacgcgc gacccttccc gctcggtgac ctgcccgccc aactcgacga acagcacctc
     7621 gatgtcggtc caacgaacat tcgccgatac cggccgggcg aatatctgtt cgagggtgcg
     7681 ctggtgcttt cgcttcatgg atgaatggta gcaaatgatg gtatcaacca acaaagcgat
     7741 gctgcaagcc atctgaccgc ccgcaccgcc accgctgcac cactggcttt gtctcttctg
     7801 ctaccgccgt cccgccagcg ccgcttcacg cgactgcgaa aaaccgtcag acgttgaacc
     7861 tgaaatggat cacgtcacca tcggcgacga tgtactcctt gccctccagc cgccatttgc
     7921 cggcctcctt ggcgccctgc tcaccgttgc cggcgacgaa gtcgtcgtag gcaatcacct
     7981 cggcacggat gaaaccgcgc tcgaagtcgg tgtggatcac cgcggccgcc ttgggtgcgg
     8041 tggcgccgat cgggatggtc caggcccgca cctccttctg tccggcggtg aaataggtgt
     8101 gcagacccag cagccggtag ccggcgcgga tcagccggtt gaggccgggc tcctcgaagc
     8161 cgagcgattc gagaaactcg gctttttcgg actcttcgag ttcggcgatc tcggcctcga
     8221 gctgattgca gatcaccacc acctcggccc cttcggccgc cgccaccgcc cgcaccgcgc
     8281 gaacgtggtc gttgtcctcc tcgccttcgg cgacattggc gatgtagagg gtcggtttga
     8341 cggtgagcag atgcagtggc cgcaacagtg ccagcgccgc aggctccagc cccagcgtgc
     8401 gcaccggtct ggcctggttc agttcgggct ggatgcgctc cagcagggca tgcagcgcaa
     8461 ttgcctcctt gtcgccaccc ttggcggcac gcgcgctgcg ggccaggttc ttttcgacgg
     8521 tgtcgaggtc ggccagcgcc agttcggtgt tgatcacttc gatgtcagac accgggtcga
     8581 cgcggttggc cacatggatg atgttgtcat cctcgaaaca gcggaccacc tgggcaatgg
     8641 catcggtctc acggatgttg gcaagaaact ggttgcccag tccctcgcct ttcgaggcac
     8701 ccgccaccag cccggcgatg tcgacgaact ccatcgtggt aggcaccacc cgctccggtt
     8761 ggacgatctg cgccagccgg tcgagccgtg gatcgggcat cggcaccacc ccggtgtggg
     8821 gatcgatggt gcagaagggg aagttggcgg catcgatgcc ggcctgggtg agtgcattga
     8881 acagggtcga tttgcccacg ttcggcaagc cgacaatgcc acatttgaat cccatgtcag
     8941 gacctattcg atgtcaggtg ggggcatgca accggctcat cgccgtcgcc cagttgccat
     9001 tgagggcgtc ggggagtgcc cgcagggcat catccatcgc ctggtcgatc gcctcgcgct
     9061 ctgccgcagt gggcggattg agcacatagg tgaccacccg tgaactgtct ccggggtggc
     9121 cgatgccaag acgcagccgg tagaagcttt tgtcaccgcc caactgctcg atgatgtcgc
     9181 gcaggccatt gtgaccgcca tggccgccgc cttgcttgaa gcgggccacg ccgggcggca
     9241 gatcgagttc atcgtggacc agcagcagac ggctgggcgg aatctggtaa aagcgcagca
     9301 aggccgccac ggcccggccg ctgcggttca tgaaggtgtc cggttgcagc aggtgaaatt
     9361 cgcaaccctg ccagaacgcg cgggcgtgac ggccatggaa cttgcgctct tcgcgcagcc
     9421 ggacgccggc cagcgccgcc agccggtcga ccagccagca gccggcattg tggcgggtgg
     9481 ccgcatagtc ggggccgggg ttgcccagcc cgacgatggc gtcgatccgc gcgctcaagc
     9541 gaaagccggg cgggagcgat caaccgccgg cttcgtcacc accacccttg gtcggcacca
     9601 ccgccaccac ctgggcgtca tggtcgccgc cctgcgacag ggccaccagt tcgacgcctg
     9661 gcggcaattg caggtcggaa aggtgaatgt gtcggcccac gccgacgtcg agcatgtcga
     9721 cttcgaggta ttccggcaga tcgcccggca gacagctgat ctccacctcg gtcagggtgt
     9781 gggcgatgcg accaccatcg cgcttgatgc cgatgcagtt ctcttcattg atgaagtgca
     9841 gcggcacatg cacgtgaatc ttgcggttca tctcgacccg catgaagtcg gcatgcatga
     9901 ccgacccctt gctggggtga cgttgcagcg ccttgagcag gaccggctca ctgctgccgc
     9961 ccaccttgag gttgatgatg tgcgtgtaga aggcctcgct ctccagcgat tttgccag
//
LOCUS       DAORCQ010000082         9809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4758_length_9809_cov_5.406807, whole genome shotgun sequence.
ACCESSION   DAORCQ010000082 DAORCQ010000000
VERSION     DAORCQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4758_length_9809_cov_5.406807"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /gene="pyrG"
                     /locus_tag="PKI23_08835"
     CDS             complement(<1..167)
                     /gene="pyrG"
                     /locus_tag="PKI23_08835"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252329.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HNN37081.1"
                     /translation="MTKYIFVTGGVVSSLGKGITSASLGAILEARGLKVTMIKLDPYI
                     NVDPGTMSPFQ"
     gene            complement(280..1035)
                     /locus_tag="PKI23_08840"
     CDS             complement(280..1035)
                     /locus_tag="PKI23_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010451297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNN37082.1"
                     /translation="MNHSLFEALLDPTLPTPAGLRVWNGSDPAARFAIHRNHLRVSLT
                     SALAQSYPVTRTLVGEPFFNAMAWQFIRQAPPRLPMLACHGAALPNFIDSFAPAATLP
                     YLADVARLEWLRIEACHAADAVELPTASLTRQLQQPATLARLKITFQPSVRLLTSRHA
                     VVSIWAAHQGRGDLRAVDPVLPESALILRPQLDVELIPLPLAAGDFIGRLLKGEPLGS
                     ALEAHLATTADFDLVETLRLLIASGAIAALESD"
     gene            complement(1032..1898)
                     /locus_tag="PKI23_08845"
     CDS             complement(1032..1898)
                     /locus_tag="PKI23_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019817838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNN37083.1"
                     /translation="MNSLPARAGVGLKADHFQRIVSDWPDIGFFEVHAENYLMAGGPH
                     HHYLTRIRERYPLSIHGVGLSLGGEAPLDVDHLDRLKALLDRYQPESFSEHLAWSSHG
                     GFFFNDLLPLPYTPVTLQRLCDHIEQTQTHLQRQILLENPATYVEFAASTMAETDFIQ
                     ELLRRSGCALLLDLSNLHLSCINHQRDTAATLRALASCPVGEIHLAGFSETQDDAGAP
                     LLLDSHDRPVAAEVWQLYRQALALLGPVATLLERDHELPPFDLLFEESQQAERLLRGL
                     DRTGATHCEPGQ"
     gene            2122..3093
                     /gene="arsS"
                     /locus_tag="PKI23_08850"
     CDS             2122..3093
                     /gene="arsS"
                     /locus_tag="PKI23_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339360.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNN37084.1"
                     /translation="MHATLPLLQPSDFPALQRQRLTTLQVNLGYRCNQSCLHCHVNAG
                     PTRKEQMEADTLALIPRVIEACGIDTLDLTGGAPELHPDFRAVVRSARALGVRVIDRC
                     NLTILFEPGQQDLAEFLAAEQVEVVASLPCYLPENVDRQRGNGVFDRSIAALQRLNAL
                     GYGRAGSGLTLNLVFNPQGPSLPPSQSALQADYARELYAQFGIVFNQLFVLANMPIQR
                     FGSTLISKGSFADYLRLLRENFAAANLEQVMCRTLVSVDWQGHLHDCDFNQQLGLPLP
                     AFDGLDSPPHLRDLLRVALEGRPIQVADHCYGCTAGQGSSCGGSLNQ"
     gene            3112..5268
                     /locus_tag="PKI23_08855"
     CDS             3112..5268
                     /locus_tag="PKI23_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNN37085.1"
                     /translation="MRKGAIAVGLLGLVMLFFALGLHRQLTFDQLKESLGQLMAWRAA
                     EPFRSALLFFVLYLLVTALSLPGAAIMTLAAGALFGLGWGFVIVSFASSLGATLAFMT
                     ARYLLHDAVQRRFGNRLQAINAGMARDGALYLFTLRLVPIFPFFIINLLMGLTPIGSW
                     TFYWVSQLGMVAGTLVYVNAGTQLAQLTDLSGIFSPALLLSLALLGFFPLLSKRLLQT
                     WQRRRVYARWRRPRRFDRNLLVIGGGAAGLVTAYIAATVKARVTLIEAHKMGGDCLNH
                     GCVPSKALIEVARLAHAIRHAPSQGLLVGEPVVDFRTVMARVQAVIRQIEPHDSIERY
                     TGLGVEVLEGRARLVDPWTVAVAMKQGGERRLTARSIVLATGAQPLVPPLPGLEEVGY
                     LTSDTLWAQFATLDVLPGRIVVLGGGPIGCELAQSLARLGAEVTQIERSERILQQEDE
                     EVALLLQDALGQEGVILLTGHLALHCERDGERKFIVVADAEQQTRRIEFDALICAVGR
                     VARLTGYGLEELGIATDRRVQTNDYLETLYPNILAAGDLVGPWQFTHAAAHQAWYAAV
                     NALFGQFWRFRVDHSVLPRATFVDPELARVGLNEQEARAQGVPYEVTRYELAELDRAI
                     IDDKRRGFVKVLTVPGRDRILGVTIVGEQAAELIAEFVLAMQQGVGLNALLGTLHIYP
                     TLAEANKYVAGQWRQAHAPQRLLAWAARYHRWRRGG"
     gene            5268..5942
                     /locus_tag="PKI23_08860"
     CDS             5268..5942
                     /locus_tag="PKI23_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNN37086.1"
                     /translation="MRLCIIVPILNEAQRLPRLLDELAPLQRQGVEVILVDGGSDDGS
                     AGMVEAAGFTLVRSARGRARQMNAGAALARGDTLLFLHADTRLPAQAIQSIEQALAAG
                     TACWGRFDVTLSGRSCLLWVVARMMNWRSRCSGIATGDQAIFVTRAVFDAVGGFPDQP
                     LMEDIELSRRLKRRSRPACLRNRVLTSGRRWHGKGPWRTILLMWRLRWAYWRGTPAEQ
                     LARRYR"
     gene            complement(5970..8081)
                     /locus_tag="PKI23_08865"
     CDS             complement(5970..8081)
                     /locus_tag="PKI23_08865"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009407459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNN37087.1"
                     /translation="MKQFFYPETAARALLLLFLCITSVTAAPTPVALLDYKGEFSDIA
                     EEVLIHLQQNHYRHVDIDDQLSSKLLDRYLKLLDPTRSHFLTRDIEEFETVRKLLDDG
                     LRTGNVEPAFHIYNRFQQRLAERLTYSLDLLEKQPQTLDLTQDERLDLDREQAPWPRD
                     SAAQQQLWRRQLKNSLITLRLADDHEDPAKANQVLIKRFHNQLSRLQQTGSRDVFQLY
                     MDALTSNFDPHTQYFSPRLSENFQINMSLSLEGIGAVLSSEDEEIKIVRLVPKGPADK
                     SGQLHSGDRIIAVGQGAEGELVDIVGWRLDDVVEKIRGKKGTQVRLKVIPHDRTEGGS
                     KIVTIERNEVKLEEQAAQSKIFPVDQNGSQTRIGVITLPAFYADFEAQRRGDRNYKST
                     TRDVARLLGELKAAKVDGIVLDLRNNGGGSLQEVNSLIGLFIQQGPTVQVRLNNGRTE
                     LQGDPDPRIFYSGPLAVLVNRLSASASEIFAGAIQDYQRGLVIGEQTFGKGTVQSLID
                     LNEGQIKITTAKFYRISGQSTQNRGVTPDISFPSLVDTKEIGESALPDALPWDTIAAT
                     PFRIFEDHQRLLPLLRTNHAQRSDQNPEFIYLIRRAELQKRLRDQKELSLNEKTRDSE
                     REKIERERLDLENQLRASRGEAPLSSVTQIAKSEEKDELNPDPGKDPLIVESGHILVD
                     YMALDKQQHLVRQDGSAQDQR"
     regulatory      8211..8430
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8554..>9809
                     /locus_tag="PKI23_08870"
     CDS             8554..>9809
                     /locus_tag="PKI23_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNN37088.1"
                     /translation="MNAFRLKTLLTLSTLVAVEAAAAPPPMSLVITPTRTARTVDASL
                     AAVTVIDRAQIERQQPRSLQELLTGMPGVSLSNSGGIGQLTTLSLRGTEPDHLLVLVD
                     GVKVGSATTGMTAFQDLPVEQIERIEIVRGPRSSLYGSEAIGGVVQIFTRKGRGPIKP
                     TVALGAGSQGGYQASAGVSGGSDASWFSLNANGFGNSGDNACYSSQGCFTVEPDKDGY
                     HNESAALRAGHRFANGAELDGHWLQAVGENEYDGFYNRSEVVEQVVGGSLRFAPLEPW
                     LVTLRAGRSRDESDNYSGQTFQTRFDTLRDSGSWQNDLTLHPGQLLTLGVDWQQDRID
                     STTLYDQTSRRNEGLFGQYQGQWGAHDLQLSLRRDDNEQYGHHDTATAAWGYGFGNGV
                     RLFASHGTAFKAANFNDLYYPGYSNP"
BASE COUNT         1586 a         2982 c         3367 g         1874 t
ORIGIN      
        1 tgctggaacg ggctcatggt gcccggatcg acgttgatgt agggatcgag cttgatcatg
       61 gtgaccttca ggccacgcgc ttcgagaatc gcgccgagcg aggccgaggt gatgcctttg
      121 ccaagggagg agaccacgcc tccggtgacg aaaatgtact tggtcatgca ggatcctgtt
      181 ggaaaccgaa gggcgttgcg cgatccgtca tggatcggcc gggagcgggg cgcgagtata
      241 tcaaatcggc ggcagcggtt caggggtggc gtcggcgact caatcactct ccagcgcagc
      301 gatcgctcca ctggcgatca gcagccgcaa cgtttcgacc aggtcgaagt cggcagtggt
      361 ggccagatgg gcttcgagtg cgctgccaag gggctctcct ttcagcaggc gcccgatgaa
      421 gtcgccggca gccagcggca gcggaatcag ctcgacatcg agctgaggac ggaggatcag
      481 cgcgctctcg ggcagaaccg gatcgacggc gcgcagatcg ccgcggccct gatgcgcggc
      541 ccagatcgac accacggcat ggcgggaggt caacagcctg accgacggct gaaaggtgat
      601 cttcagccgt gccagcgttg ccggctgctg cagctgccgg gtcaggctgg cggtcggcag
      661 ctcgacggca tcggccgcgt gacaggcttc gatgcgcagc cactccagtc gggcgacatc
      721 ggcgaggtag ggcagggtcg cggcgggcgc gaaactgtcg atgaagttgg gcagcgcagc
      781 gccatggcag gccagcatcg gcaggcgcgg tggtgcttgc cggatgaact gccaggccat
      841 ggcgttaaag aatggctcgc cgaccagcgt ccgggtgacc ggatagctct gcgccagggc
      901 gctggtcagc gagacccgca ggtggttacg atggatggcg aaacgggcgg cggggtcgga
      961 accgttccag acccgcagtc cggctggagt gggcagcgtg gggtcgagca gcgcttcgaa
     1021 cagggagtga ttcactggcc cggctcgcag tgcgtggctc cggtccgatc gaggccgcgc
     1081 agcagccgct cggcctgctg gctctcttcg aacagcagat cgaagggcgg cagctcatgg
     1141 tcacgctcca gcagcgtcgc caccggaccg agcagtgcca gcgcctgccg atagagctgc
     1201 cagacttcag ccgccacggg ccgatcgtgg ctgtcgagca gcagcggggc gccggcgtca
     1261 tcctgcgttt cgctgaagcc ggccagatgg atctcgccga ccgggcagct cgccagggcg
     1321 cgcagagtgg cagcggtgtc acgctggtgg ttgatgcagg agagatgcag gttgctcaga
     1381 tcgagcagca gagcgcagcc actgcggcgc agcaactcct gaatgaaatc ggtttcggcc
     1441 atcgtcgagg cggcgaattc gacataggtg gccggatttt ccagcaggat ttgccgttgc
     1501 agatgggtct gggtctgttc gatgtggtcg cagagccgtt gcagcgtcac tggggtgtag
     1561 ggcagcggca gcaggtcgtt gaaaaaaaag ccgccatggc tcgaccaggc cagatgttcg
     1621 gagaagctct cgggctggta gcggtcgagc agcgccttga gccggtcgag gtggtcgaca
     1681 tcgagtggcg cctcgccgcc cagcgacagc ccgacgccat ggatcgacag cgggtagcgc
     1741 tcacggatgc gggtcagata gtggtggtgc gggccgccgg ccatcaggta gttttcggca
     1801 tggacctcaa aaaagccgat gtcgggccag tcgctgacga tccgttgaaa atggtcggcc
     1861 ttcaggccca cgcccgcgcg tgccggcagg ctgttcatgg gcttttcagc aaaggggagg
     1921 ccgtgcggca atggtggttg tgacgacatc cgctgtgatg cgttgatctg gtttgaatgc
     1981 gctgcgtggg gcgccacatt ggacccccgg tccggagtgg tcaagagtga gcgtggtcga
     2041 atctctttgt gctgttgtaa tggcgcgccg aatgttctgc aatgcgctct tgtcacacca
     2101 accgaatgta ccggggccgc catgcatgcc accctgccac tgttgcagcc gagtgatttt
     2161 cctgccctgc agcgccagcg gctgaccacc ctgcaggtca acctcggtta ccgctgcaat
     2221 caatcctgcc tgcactgcca tgtcaatgcc ggtccgaccc gcaaggagca gatggaggct
     2281 gacaccctgg cgctgattcc acgggtgatc gaggcctgcg gcatcgatac gctcgatctg
     2341 accggtggcg cgccggagct gcaccccgac ttcagggcag tggtgcgcag cgcgcgggcg
     2401 ctcggtgtca gggtgatcga ccgttgcaac ctgacgatcc tgttcgagcc cggacagcag
     2461 gatctggccg agtttctggc ggccgagcag gtcgaggtgg tggcatcgct gccctgttat
     2521 ctgcccgaga atgtcgaccg ccagcgcggc aatggcgtgt tcgacaggag cattgccgcg
     2581 ctgcagcggc tcaacgcact cggctatggc cgcgccggca gtggcctgac cctcaatctg
     2641 gtcttcaatc cgcaggggcc atcgctgcca cccagccaga gtgcgttgca ggccgactat
     2701 gcgcgtgagc tttatgcgca gttcggcatc gtcttcaatc agctcttcgt gctggccaac
     2761 atgccgattc agcgcttcgg ttcgacgctg atctccaagg gcagcttcgc cgactacctg
     2821 cggctgctgc gggagaattt cgccgccgcc aacctcgagc aggtcatgtg ccgcacattg
     2881 gtcagtgtcg actggcaggg ccatctgcat gactgcgact tcaatcagca gttgggactg
     2941 ccgctgccgg cgttcgatgg tctggactcg ccgccacatc tgcgcgatct gctgcgcgtt
     3001 gcgctggagg gacggccgat ccaggtggcc gaccactgct atggctgcac cgccggacag
     3061 ggcagcagtt gcggtggttc gctgaatcag taggcgtcag gagttcattc gatgcgaaaa
     3121 ggcgcgattg cggtcggact gctggggctg gtcatgctgt tcttcgcgct cgggctgcac
     3181 cgccagttga ccttcgatca gctcaaggag agtctcggtc agctcatggc atggcgtgcc
     3241 gccgagccgt tccgcagcgc gctgctgttc ttcgtgctct acctgctggt gacggcgcta
     3301 tcgctgcccg gcgcggccat catgacgctg gcggcggggg cgctgttcgg cctgggctgg
     3361 ggcttcgtga tcgtctcctt cgcctccagt ctgggtgcga ccctggcctt catgaccgcg
     3421 cgctacctgc tgcatgatgc cgtgcagcga cgcttcggca atcggctgca ggcgatcaat
     3481 gccggaatgg cccgcgatgg cgccctctat ctcttcacgc tgcggctggt gccgatcttt
     3541 ccgttcttca tcatcaacct gctgatgggg ttgacgccaa tcggcagctg gaccttctac
     3601 tgggtcagtc agctcggcat ggtggcgggc acgctggtct atgtgaacgc cggcacccag
     3661 ttggcccagc tcactgatct gtcaggcatc ttctcgcctg cgctgctgct gtcgttggca
     3721 ctgctcgggt tttttccgct gctgagcaag cggctgttgc agacttggca gcgacggcgt
     3781 gtgtatgccc gctggcgccg accccgccgt ttcgaccgca atctgctggt gatcggcggt
     3841 ggcgcggcgg gtctggtcac tgcgtacatc gctgcgacgg tgaaggcgag ggtgaccctg
     3901 atcgaagcac ataagatggg gggtgactgc ctcaaccacg gctgtgtgcc gagcaaggcg
     3961 ctgatcgaag tggcccgtct ggcccatgcc atccgccatg cgccaagcca gggtctgctg
     4021 gtgggtgagc cggtggtcga ctttcgcacg gtgatggcgc gggtgcaggc ggtgattcgg
     4081 cagatcgaac cgcatgacag catcgagcgc tacaccgggc tgggcgtcga ggtgctcgaa
     4141 ggccgggccc gcctggtcga tccgtggacg gttgcggtgg caatgaagca ggggggcgag
     4201 cggcggctga ccgcacgcag catcgtgctc gccaccggtg cccagccact ggtgccgccg
     4261 ttgccggggc tggaggaggt gggttatctc accagcgaca ccctgtgggc gcagttcgcc
     4321 acgctcgatg tgctgcctgg acggatcgtg gtgctgggcg gcggtccgat cggctgtgag
     4381 ctggcgcaga gccttgcccg gctgggggcg gaggtgaccc agatcgagcg ctccgagcgc
     4441 atcctgcagc aggaggatga agaggttgcc ctcctgctgc aggatgcgct cggacaggag
     4501 ggtgtgatcc tgctcaccgg ccatctggca ctgcattgcg agcgcgacgg ggagcgcaag
     4561 ttcatcgtgg tcgccgatgc agagcaacag acgagacgca tcgaattcga tgcactgatc
     4621 tgcgcggtcg gccgtgttgc ccggctgacc ggttatggtc tggaagagct gggcatcgcg
     4681 accgaccgca gggtgcagac caatgactac ctcgagaccc tctaccccaa catcctggcc
     4741 gccggcgacc tggtggggcc ctggcagttc acccacgcag ccgcgcatca ggcctggtat
     4801 gccgccgtca atgcgctctt tggccagttc tggcgcttcc gcgtcgatca ctcggtgctg
     4861 ccgcgcgcca ccttcgtcga tccggagctg gcgcgtgtcg ggctcaacga gcaggaggcc
     4921 agggcccagg gggtgcccta cgaggtcacc cgttatgaac tggccgagct ggaccgtgcc
     4981 atcatcgatg acaagagacg gggcttcgtc aaggtgctga cggtgcctgg gagagaccgc
     5041 atcctcggtg tcaccatcgt cggtgagcag gctgccgagc tgatcgccga gttcgtgctg
     5101 gcgatgcagc agggggtggg tctgaacgcg ctgctgggca ccctgcacat ctatccgacg
     5161 ctggccgaag ccaacaagta tgtcgccggg cagtggcgac aggcccatgc gccgcagcgg
     5221 ctgctggcct gggcggcgcg ttaccaccgc tggcggcggg gtggctgatg cggctctgca
     5281 tcatcgtgcc gattctgaac gaggcgcagc ggttgccccg gttgctggat gagctggcgc
     5341 cgctgcaacg gcagggtgtc gaggtgatcc tggtcgatgg cggcagtgac gatggctccg
     5401 ccgggatggt cgaggcggcc ggtttcaccc tggtccgctc tgcgcgcggg cgcgcccggc
     5461 agatgaacgc cggcgcggcg ctggcccgtg gcgacaccct gctgtttctg catgccgaca
     5521 cccgcctgcc ggcgcaagcc atccagtcga tcgagcaggc gctggcggct ggcactgcct
     5581 gctggggccg cttcgatgtc accctttcgg gtcgctcctg cctgctgtgg gtggtggcgc
     5641 gaatgatgaa ctggcgctcg cgctgctccg gcatcgccac cggcgaccag gcgatcttcg
     5701 tcacccgggc ggtctttgat gcggtcggtg gctttcccga ccagccgctg atggaggaca
     5761 tcgaactctc ccggcgactc aagcggcgct cgcgccctgc ctgtctgcgc aaccgggtgc
     5821 tcacctcggg ccgccgctgg cacggcaagg gcccatggcg gacgattctg ctgatgtggc
     5881 ggctgcgctg ggcctattgg cgcggcacgc cggcggagca gttggcgcgc cgctatcgct
     5941 gaagccaggt cggcagggct gcgggcgtgt taccgctgat cttgcgctga gccatcctgc
     6001 ctgaccaggt gttgctgttt gtcgagcgcc atgtagtcga ccagaatgtg gccgctctcg
     6061 acgatgagcg gatccttgcc cggatcgggg ttgagctcat ccttctcttc gctcttggcg
     6121 atctgggtca ccgagctgag cggcgcctca ccgcggctgg cacgcagttg attctccaga
     6181 tcgagccgtt cacgttcgat cttctcacgt tcactgtcac gcgttttttc gttcagtgag
     6241 agctctttct gatcacgcag ccgtttttgc agttcggccc gcctgatcag atagatgaac
     6301 tccgggttct ggtcgctgcg ctgagcatgg ttggtgcgca gcagcggcaa caggcgctgg
     6361 tgatcctcga agatgcgaaa gggggtcgcg gcgatggtgt cccacggcag tgcatcgggc
     6421 agggcgcttt cgccgatctc cttggtgtcg accaggcttg gaaagctgat gtcaggggtc
     6481 acgccgcggt tctgggtgct ctgccccgag atgcggtaga acttggcggt ggtgatcttg
     6541 atctgcccct cgttgaggtc gatcagggac tgcaccgtgc ccttgccgaa ggtctgttcg
     6601 ccaatgacca gtccgcgctg ataatcctgt atggcgccgg cgaagatctc cgaggccgag
     6661 gcgctcaggc ggttgaccag caccgccagc ggaccgctgt agaagatgcg cgggtcagga
     6721 tcgccctgca attcggtgcg gccattgttg agtcgcacct ggacggtggg gccctgctgg
     6781 atgaacaggc cgatcagtga gttgacctcc tgcagcgagc cgccgccgtt gttgcgcaga
     6841 tcgagcacga tgccgtcgac cttggcggcc ttgagttcgc caagcagcct ggccacatcg
     6901 cgggtggtgc tcttgtagtt gcgatcgcca cggcgctggg cctcgaaatc ggcatagaag
     6961 gcgggcaggg tgatgacgcc gatgcgggtc tggctgccgt tctggtcgac cgggaagatt
     7021 ttcgactggg ccgcctgctc ttcgagcttg acctcgttgc gctcgatggt gacgatcttg
     7081 ctgccaccct cggtgcggtc atgcgggatc accttcagac gcacctgcgt gcccttcttg
     7141 ccgcgaatct tctcgaccac gtcatccagc cgccagccga cgatgtcgac cagctcaccc
     7201 tcggcaccct gacccaccgc gatgatccgg tcgccgctgt gcaactgccc cgatttgtcg
     7261 gccggaccct tcggcaccag ccggacgatc ttgatctcct catcctccga gctgagtacg
     7321 gcgccgatgc cttcgagcga gaggctcatg ttgatctgga agttctccga cagccgaggg
     7381 gagaagtact gggtgtgcgg gtcgaagttg ctggtcaacg catccatgta gagctggaac
     7441 acatcgcggc tgccggtctg ctgcaggcgg ctgagctggt tgtgaaagcg cttgatcagc
     7501 acctggttgg ccttggccgg atcttcatgg tcgtcggcca gccgcagcgt gatcaggctg
     7561 ttcttgagct gccggcgcca cagctgctgc tgcgcggcgc tgtcccgcgg ccagggggcc
     7621 tgctcgcggt cgagatcgag gcgctcgtct tgcgtcagat ccagcgtctg tggctgcttt
     7681 tcgagcaggt cgagcgaata ggtgagccgt tcggccagcc gttgctggaa gcggttgtag
     7741 atgtgaaagg ccggttcgac attgccggtg cgcaggccgt catcgagcag tttgcgcacc
     7801 gtctcgaatt cctcgatgtc acgggtcaga aaatggctcc gtgttggatc gagcagtttc
     7861 agatagcggt cgagcagctt gctggagagc tggtcatcga tgtcgacatg ccggtaatgg
     7921 ttctgctgca ggtgtatcag cacctcttcg gcgatgtcgc tgaactcccc cttgtagtcg
     7981 aggagggcga ccggtgtggg tgcggccgtg acgctggtga tgcacaaaaa gagcagcagc
     8041 agtgcgcggg ccgccgtttc aggataaaaa aattgcttca tgaatccagg ggtgcgatcg
     8101 ggaatggaag gagtgatgcg tttttttgca actgctgccg cgctgaaaag cttaactgat
     8161 tcggttgcgg ctcgccatgc gtgggtttga caccgacgga tcggatacct aatatccgcc
     8221 gcgcttctgg tgtcccaacg gcattcgccg gcgggattaa acgggaagcc ggtgcgtccc
     8281 tcgtcacgag gtgaccagtc cggcgctgcc cccgcaacgg taagcgagtc gaggtgccgc
     8341 acgatgccac tgggttcgac ctgggaaggc gcggcaccgg gagcaatcca ctcgcaagcc
     8401 cggagaccgg ccagttgctc aggtacggca atcgcggagg gcgatgcgcg gcagtgggcg
     8461 gtgcgccggt gggcgtgccc tgcgtccggt tttccctttg cggccgagca cttttttcaa
     8521 aaagcgcgcg ggtgtgcgcg aagggaacct ccaatgaatg cattcagact caaaaccctg
     8581 ttgacactgt cgacgctggt ggccgttgaa gctgccgccg cgccgccgcc gatgtcgctg
     8641 gtgatcacac caacacgaac cgcccgcacc gtcgatgcct ctctggccgc agtgaccgtc
     8701 atcgatcgcg cgcagatcga gcgccagcag ccgcggtcgc tgcaggagct gctgaccggt
     8761 atgccgggcg tctcgctttc caacagtggc ggcattggcc agctcaccac gctgtcgctg
     8821 cgtggcaccg agccggacca tctgctggtg ctggtcgatg gcgtgaaggt cggctcggcg
     8881 accaccggca tgaccgcctt ccaggatctg ccggtggagc agatcgagcg catcgagatc
     8941 gtccgtggtc cgcgctccag cctctatggc tccgaggcga tcggtggcgt ggtgcagatc
     9001 ttcacccgca agggccgcgg gccgatcaaa cccacagtgg cactgggtgc cggttcgcag
     9061 ggcggttatc aggcatccgc cggcgtttct ggcggcagtg atgcgagctg gttcagcctc
     9121 aacgccaacg gtttcggcaa cagtggcgac aacgcctgct acagcagcca gggctgcttt
     9181 acggtggaac cggacaagga tggctaccac aacgaatcgg ccgcgttgcg ggccgggcac
     9241 cgctttgcca atggcgccga actggatggc cactggctgc aggcagtcgg cgaaaacgaa
     9301 tatgatggtt tctacaaccg ttccgaagtc gtcgagcagg tggtgggcgg ctcgctgcgt
     9361 tttgcgccgc tggagccctg gctggtcacg ctgcgcgccg ggcgcagccg cgatgagtcg
     9421 gacaattaca gtgggcagac ctttcagacc cgcttcgaca cgctgcgcga cagcgggtca
     9481 tggcagaacg atctgacgct gcatcccggg caactgctga cgctgggcgt cgactggcag
     9541 caggaccgga tcgacagcac caccctctat gaccagactt cacgccgcaa cgagggcctg
     9601 ttcggccagt atcaggggca gtggggtgcc catgacctgc aactgagtct gcgccgcgac
     9661 gacaacgaac agtatggcca ccacgacacc gccaccgcgg cctggggtta tggctttggc
     9721 aacggcgtgc ggctgttcgc cagccatggc acggcgttca aggcggccaa cttcaatgac
     9781 ctctactatc ccggttacag caatcccga
//
LOCUS       DAORCQ010000083         9788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4769_length_9788_cov_5.852358, whole genome shotgun sequence.
ACCESSION   DAORCQ010000083 DAORCQ010000000
VERSION     DAORCQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4769_length_9788_cov_5.852358"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..188)
                     /locus_tag="PKI23_08875"
     CDS             complement(66..188)
                     /locus_tag="PKI23_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37089.1"
                     /translation="MDEVLRRPALRSPSLDSAARLLLAQMERDPLLHHSTGLSV"
     gene            415..1737
                     /locus_tag="PKI23_08880"
     CDS             415..1737
                     /locus_tag="PKI23_08880"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNN37090.1"
                     /translation="MSRVNLGICGLGTVATGVIRVLERNGGEIARRAGTALHIAHIGA
                     RRDHPGCDTSAYRVSRDLFAVVRDPEVDVVVELIGGCDTARSLILEAIAAGKHVVTAN
                     KALIALHGNEIFAAARERGVMVAFEAAVAGGIPIIKTLREGLSANRIEWLAGIINGTG
                     NFILSEMRDKGRAFDDVLKEAQALGYAEADPTFDVEGIDAAHKLTILASIAFGIPLNF
                     PAVYTEGISRLTPMDVAYAEELGYRIKHLGIARRSAKGVELRVHPTMIPARRLLANVN
                     GVKNGLMIQGDAVGPTLFYGAGAGAEPTASAVIADLVDVARQLGADCGRQVPSLAFHA
                     DALVELPMVPIEAVESPFYLRIHALDRAGVLAKVATILSRRGISIEALIQKEPPIIPE
                     SQEIAVPIIMLTHHVVERELNQAIAEIEQLDEVLEPVCRIRVEYLATE"
     gene            1758..2843
                     /gene="thrC"
                     /locus_tag="PKI23_08885"
     CDS             1758..2843
                     /gene="thrC"
                     /locus_tag="PKI23_08885"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNN37091.1"
                     /translation="MATSSRYVGLIDRYRDRLPVHDDTRFISLGEGNTPLIRLNNLPR
                     LLGREVDIYVKYEGLNPTCSFKDRGMTMAVTKAVEGGSRAIICASTGNTSASAAAYAA
                     RAGITAFVLIPEGKIALGKLAQTMMYGAAIIQIRGNFDVGMALVKQVADEAPVTIVNS
                     INPYRLQGQKTAAFEIVEELGLAPDFHCLPVGNAGNITAHWIGYSEYQHDGIINSRPR
                     MLGYQAAGAAPFVTGQMVDAPETVATAIRIGHPQSWDKAWSAQRESQGWFDALSDEEI
                     LQAQRLLAMHEGIFCEPASAASVGGAMRDIRAGKIPEGSKVVCTLTGHGLKDPDSAIA
                     QCQGTETLLTVDATLDAVRRVILERIG"
     gene            2860..4599
                     /gene="recJ"
                     /locus_tag="PKI23_08890"
     CDS             2860..4599
                     /gene="recJ"
                     /locus_tag="PKI23_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNN37092.1"
                     /translation="MAALRRRIQRHAGATRDGLLPAQPEALLAALYRHRGLGEGAVNQ
                     PLELAGLLPPADLKGIDAAVELVIDAITQGRRILVVGDFDADGATSCAVAVTGLRLLG
                     ATEVGYLVPNRFEFGYGLTPEIVAIAARSAPHLLITVDNGISSMAGVLQAQAAGIRVL
                     ITDHHLPGAELPPADAIVNPNQPGCRFASKALAGVGVIFYLLLAVRGRLRDEGWFGAG
                     VQAPNLAELLDLVALGTVADVVPLDGNNRILVEQGLRRIRAGRARPGIQALLEVSGRP
                     SDSVVSSDLGFAVGPRLNAAGRMDDMTLGIECLLAPDPARARQLAQLLDRFNQSRRET
                     ESLMHEEALLELEQQGELAAEALPVGLALYRPGWHQGVIGILASRLKERYHRPVIIFA
                     QGGEGELKGSARSIPGLHIRDLLDAMATAQPDLIRRFGGHAMAAGLSIADGDYSRFAT
                     LFDQTARQWIAPEVLEGIVLSDGILPVAALTLEVARQLRYGGPWGQHFPEPLFEGEFE
                     LLQRRIVGGHHLKMLLASAEGGEPLDAIAFRFDLDQLEQLPNRLRLAYRLDLNRYRGE
                     TRLQLVVEHWEAT"
     gene            4682..5116
                     /locus_tag="PKI23_08895"
     CDS             4682..5116
                     /locus_tag="PKI23_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNN37093.1"
                     /translation="MKARVKWAGDVMFVAESGSGHAVVLEGPPEQGGRNVGIRPMELL
                     LMGVGGCSSYDVVSILRKSRQQVTDCVAELTAERADEVPAVFTKIHLHFIVTGHGLKE
                     AQVKRAVELSAEKYCSASIMLGRAGVEVTHGYQIIELDSSHG"
     gene            5109..5504
                     /locus_tag="PKI23_08900"
     CDS             5109..5504
                     /locus_tag="PKI23_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNN37094.1"
                     /translation="MAERPAATRGMRHVALFVRAFDATERFYVELIGMKVEWRPDSDN
                     VYLTSGHDNLALHRAAEGQAAGDGRLDHIGFMLARAEDVDAWHLFLQQAGVEIVKPPR
                     THRDGARSLYCRDPDGGLVQLIHHPPLAL"
     gene            5581..6375
                     /gene="speD"
                     /locus_tag="PKI23_08905"
     CDS             5581..6375
                     /gene="speD"
                     /locus_tag="PKI23_08905"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNN37095.1"
                     /translation="MNENRLRLHGFNNLTKALSFNIYDICYAKTEAQKRAYIEYIDEL
                     YNADRLTTILEGVVEIIGATVLNISREDYDPQGASVTMLVADPTTPPSEHLITEAPGP
                     MPRTVLGHLDKSHLTVHTYPESHPDEGISTFRADIDVATCGLISPLKALNYLIHAFES
                     DIVTVDYRVRGFTRDVDGVKHFIDHEINSIQNYFSDDTLDRYQMIDVNVYQENIFHTK
                     MLLKEFNLDNYLFGTGSSELSEREQAEIRKQLHREMQEIFYGRNIC"
     gene            complement(6459..7706)
                     /locus_tag="PKI23_08910"
     CDS             complement(6459..7706)
                     /locus_tag="PKI23_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNN37096.1"
                     /translation="MTQSHLNPPVIRTLVSGMTALLLCLHGSSSSAAATSIAEAISQG
                     QAKLNLRYRYETVDEQNKAREAAASTLRTRLNFTTAAYQGFTGQIEVDDVSAIGGETY
                     NSTVNGRTLYPIVADPTATEVNQAWLAYKFGKNDLKYGRQRINLDNQRFVGGVAWRQN
                     EQTFDGITLTNTSIDKLNLFYGYITNVNRIFGPDDGVNLADLPGEVHLINVGYEGLPI
                     GKLSGYGYLLDFDDTASAALSSATYGVRLATAKPLTDYKLLYALEYASQSDHGSNPNE
                     FERDYMLVEGGVALPLATVKLGYELLGSDNGTGFSTPLATLHAFQGYADRFLTTPANG
                     VEDRYLSIGGKIEQVDLTYIYHDYRSDTGGMDYGNESDLVISRKFGKHYSLMLKYANY
                     SADTVNASLFDTEKLWLMASADF"
     gene            complement(7788..>9788)
                     /locus_tag="PKI23_08915"
     CDS             complement(7788..>9788)
                     /locus_tag="PKI23_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNN37097.1"
                     /translation="HTEGFEAALAAAQASAPDRASTAAACGVDEAAIARLFDWFGDTT
                     RVVTLFSQGINQSSSGVDKGNAIINCHLASGKIGKPGAAPFSITGQPNAMGGREVGGM
                     ANTLAAHMEYENPADLDRLRRFWDAPALAHQPGLKAVELFQAVADGRVRALWIAGTNP
                     AVSLPEANRVRQALQQCEFLIVSDCEGRTDTSQHAHVLLPAAAWGEKDGTVTNSERRI
                     SRQRAFLPLPGEARPDWWIFTQVARALGFGSAFDYDTPQQIFREHARLSSFENDGQRD
                     FDLSGLSMLDAAQYDALQPIQWPVTPTHPDGCARLFSDGRYYTPSGRARLIPITPHLS
                     VARLDAPHPFLLNTGRIRDQWHTMTRSGRSVRLLSHIAEPFVEIHPEDAKTLGVGERA
                     LLQVHNHHGSVTLRAQISDAVQRGQLFAPIHWNDQFASAARIGSLVAAVTDPISGQPE
                     FKQSAVAVEPFAATWHGFVLSREPFTTDQIDYWCRGPIEGGHCHELAGKQPINDWRGW
                     FTARLPATDPMLESSWIEFQDAEAGRYRAARFIGGRLDSLIFIDRTPQPAERTWLLER
                     FACGQIEPSARPALLAARPAVAQPDAGRTICVCFNVGLNTIEQAIHSQRLCTVEVIGN
                     RLRAGTNCGSCLPELRAILSRSATPSAATQDCQAQNRHTDQC"
BASE COUNT         1727 a         2943 c         3287 g         1831 t
ORIGIN      
        1 agctttttgg ggacagacca cggtttccca gtcctgacca aggtcgattg cttatccgca
       61 acagatcata ccgacaaacc agtggagtga tgcaacagcg ggtcgcgctc catctgcgcc
      121 agcagcagcc gggcagcgct gtcgagtgat ggcgagcgca gggcagggcg tcgaagcact
      181 tcatccatgc tgtcagccac ccgattcgca ggcgcagagg gttgaacggt cggtttggga
      241 gccggaaaga ttccgcatcg ccaggctcgc ctcaacagcg tgatcgcgat cgcatgacgt
      301 ggtgagatcg gagttttcaa cgctctgcca gccacctctg ccgtggcaag gcttctggat
      361 gccatggccc gcggctacaa tgcgcgcttt cggcgacaaa gcgggtggtg ccttttgagc
      421 agagtcaatc tgggaatttg cggcctggga acggtcgcga cgggcgtgat cagggtgttg
      481 gagcgcaatg gcggcgaaat tgcccgccgc gccggcactg cattgcacat tgcccacatc
      541 ggtgcgcggc gggaccaccc gggctgcgac acttcggcct accgcgtcag ccgtgatctg
      601 ttcgccgtgg tgcgcgaccc ggaggtcgat gtggtggtgg agctgattgg tggctgcgac
      661 accgcgcgca gtctgattct cgaggccatt gcggccggca agcatgtggt gacggcgaac
      721 aaggcgctga ttgcgctgca tggcaacgag attttcgccg cggcccgcga gcgcggtgtg
      781 atggtcgcct tcgaggcggc ggtggcgggc ggcatcccga tcatcaagac gctgcgcgag
      841 gggctgagtg ccaatcgcat cgaatggctg gccggcatca tcaatggcac tggcaacttc
      901 attctgagcg agatgcgcga caaggggcgc gcttttgacg atgtgctgaa agaggcgcag
      961 gcgctgggct atgccgaggc cgatcccacc ttcgatgtcg aaggcatcga tgcggcgcac
     1021 aagctgacca ttctggcttc gatcgccttt ggcattccgc tcaactttcc ggccgtctac
     1081 accgagggca tcagccgtct gacgccgatg gatgtcgcct atgccgaaga gctgggttat
     1141 cgcatcaagc atctgggcat tgcccggcgc agtgccaagg gggtcgagct gcgtgtccat
     1201 ccgacgatga tcccggccag gcgactgctg gccaatgtca acggggtcaa gaatggcctg
     1261 atgattcagg gcgatgcggt ggggccgacg ctgttctatg gtgccggcgc cggcgctgaa
     1321 ccgaccgcct cggcagtgat cgcggatctg gtcgatgtgg cccgccagct cggggccgac
     1381 tgcggacggc aggtgccctc actggccttc catgccgatg cgctggtcga attgccgatg
     1441 gtgccgatcg aagcggtcga atcacccttc tatctgcgca tccatgcgct cgatcgggcc
     1501 ggtgtgctgg ccaaggtggc gaccattctc agccggcgtg gcatcagcat cgaggcgctg
     1561 attcagaagg agccgccaat cattcccgag tcgcaggaga tcgccgtgcc gatcatcatg
     1621 ctgacccacc atgtggtgga gcgtgagctg aatcaggcga ttgccgaaat cgagcagctc
     1681 gacgaagtgc tcgaaccggt ctgccgcatc cgcgtcgagt acctcgccac cgaataattg
     1741 ccgagaaagg agcccaaatg gccacatcca gccgctatgt cggcctgatc gaccgctacc
     1801 gtgaccgcct gccggtgcac gacgacaccc gtttcatcag ccttggcgaa ggcaatacac
     1861 cgttgatccg gctcaacaat ctgccgaggt tgctgggcag ggaggtcgac atctatgtca
     1921 aatatgaggg gctgaatccc acctgctcgt tcaaggaccg cggcatgacc atggcggtca
     1981 ccaaggcggt cgagggcggc agccgcgcga tcatctgcgc ttcgaccggc aacacctcgg
     2041 cgtcggcggc cgcctatgcc gcccgcgccg gcatcaccgc cttcgtgctg attccggaag
     2101 gcaagatcgc gctgggcaag ctggcacaga cgatgatgta cggggctgcc atcatccaga
     2161 tccgtggcaa tttcgatgtc ggtatggcgc tggtcaagca ggtggcggat gaggcaccgg
     2221 tcaccatcgt caactcgatc aacccctatc gtctgcaggg acagaagacc gccgcctttg
     2281 aaatcgtcga ggagctgggg ctggcgcccg actttcactg tctgccggtc ggcaatgccg
     2341 gcaacatcac tgcccactgg atcggctaca gcgaatacca gcacgatggc atcatcaaca
     2401 gccggccgcg gatgctgggc tatcaggccg ccggagcggc ccccttcgtc actgggcaga
     2461 tggtcgacgc accggagacg gtcgcgaccg cgattcgcat cggccacccg cagtcgtggg
     2521 acaaggcctg gagtgcgcag cgcgagtcgc agggctggtt cgacgcgctg agcgacgagg
     2581 agattctgca ggcgcagcgg ctgctggcga tgcatgaggg catcttctgc gagccggcgt
     2641 cggcggcctc ggtcggcggt gcgatgcgcg acatccgcgc tggcaagatt cccgaaggca
     2701 gcaaggtggt ctgcaccctg accggccacg gactgaagga tcctgacagc gccatcgccc
     2761 agtgtcaggg caccgagacg ctgctgacgg tcgatgccac cctcgatgcc gtgcgccggg
     2821 tgattctgga gcgaatcggc tgaccctgcg ggaatccgaa tggctgcatt gcgccgacga
     2881 attcaacgcc acgccggcgc gaccagggat ggtctgctgc cggcgcaacc cgaggcgctg
     2941 ctggccgctc tgtatcggca ccgtggtctg ggcgaaggcg cggtgaatca gccgctggag
     3001 ctggctggtc tgctgccgcc ggctgatctg aaaggcatcg atgcggccgt cgaactggtg
     3061 atcgatgcca tcacccaggg acggcgcatt ctggtggtcg gcgatttcga tgccgatggc
     3121 gccaccagct gcgcggtggc cgtgaccggg ctgcggctgc tgggtgccac cgaagtgggc
     3181 tatctggtgc cgaaccgctt cgaattcggc tatggcctca cgcccgagat cgtcgccatc
     3241 gccgcccgct ccgcgcctca tctgctgatc actgtcgaca atggcatctc cagcatggcg
     3301 ggcgtgttgc aggcgcaggc ggccggcatc cgggtgctga tcaccgacca ccacctgccc
     3361 ggcgccgagt tgccgccggc cgatgccatc gtcaacccga atcagcccgg ttgccgtttt
     3421 gcttccaagg ccctggccgg ggttggggtg atcttctacc tgctgctggc ggtgcgtggt
     3481 cgactgcgcg atgagggctg gttcggtgcc ggtgtgcagg cgccgaatct ggccgagttg
     3541 ctcgatctgg tggcgctggg cacggtggcg gatgtggtgc cgctggatgg caacaaccgc
     3601 attctggtcg agcaaggtct gcgccgaatc cgtgctggcc gtgcccgccc cggcatccag
     3661 gcactgctgg aggtgagtgg gcgtccgagc gacagcgtgg tcagcagcga tctgggcttc
     3721 gccgtggggc cgcggctcaa cgcggccggg cggatggatg acatgaccct cggcatcgaa
     3781 tgtctgctgg cgcccgatcc agcccgggcg cggcaactgg cgcaactgct cgaccgcttc
     3841 aatcagtcgc ggcgtgaaac cgaatcgctg atgcatgaag aggcactgct cgaactggag
     3901 cagcaggggg agctcgctgc cgaggcgctg ccggtcggtc tggcgctcta ccgtcccggc
     3961 tggcatcagg gcgtgatcgg gattctggcc tcgcggctca aggagcgcta ccaccggccg
     4021 gtgatcatct tcgcgcaggg cggtgagggt gagctcaagg gttcggcacg gtcgattccc
     4081 ggcctgcaca tccgcgacct gctcgatgcg atggcgacgg cgcagcccga tctgatccgc
     4141 cgttttggcg gccatgcgat ggcggcgggg ctcagcatcg ccgacggcga ctattcccgt
     4201 ttcgccacgc tgttcgatca gacggcacgt caatggatcg cgcccgaagt gctcgaaggg
     4261 atcgtgctga gcgatggcat tctgccggtc gccgcgctga cactggaggt ggcacggcag
     4321 ctgcgctatg gcggcccctg ggggcagcac tttcccgagc cgctgttcga gggggagttc
     4381 gagctgctgc agcgacgcat cgtcgggggt caccatctga agatgctgct ggcctcagct
     4441 gagggtggag agccgctcga tgccatcgcc tttcgcttcg atctcgacca gttggagcag
     4501 ctgccgaacc ggctgcgact ggcctaccgg ctcgacctca accgctatcg gggcgagacg
     4561 cggttgcaac tggtggtcga gcattgggaa gcgacctagg cggcagttcg atcagcgcaa
     4621 tgtcatcttg tcgggcgtgg tttatggtac cctttgcgcc tctttgcggg gggcgaggtt
     4681 gatgaaagcg cgggtgaagt gggcgggcga cgtcatgttc gtggccgagt cgggcagtgg
     4741 tcatgcagtg gtgctcgaag gaccgccgga gcagggtggc cgcaacgtcg gcatccggcc
     4801 gatggagctg ctgctgatgg gggttggtgg ctgttcctct tacgacgtgg tgtcgattct
     4861 gcgcaagtcg cggcagcagg tgaccgattg cgttgcggaa cttaccgccg aacgtgccga
     4921 cgaggtgcca gcggtcttca ccaagattca cctgcacttc atcgtcaccg gccatggcct
     4981 caaggaggca caggtgaagc gggcggtcga gctctccgcc gaaaagtact gctcggcgtc
     5041 gatcatgctg ggccgggccg gcgtcgaagt gacgcacggg tatcagatca tcgaactgga
     5101 cagcagccat ggctgagcga cccgctgcca cccgcggaat gcgccatgtc gcgctgttcg
     5161 tgcgggcctt cgatgcgacc gagcgattct atgtcgagct gatcggcatg aaggtggagt
     5221 ggcgccccga ctccgacaat gtctacctga cttcggggca tgacaatctg gcgctgcacc
     5281 gcgctgccga gggtcaggcc gccggcgatg gtcggctcga ccacatcggc ttcatgctgg
     5341 cgcgggccga ggatgtcgat gcctggcatc tttttttgca gcaggccggt gtcgagatcg
     5401 tcaagccgcc gcgcacccac cgcgatggtg cacgcagcct ctactgccgc gaccccgatg
     5461 gcggtctggt gcagttgatc caccacccgc cgctggccct gtaacaatct ttccattgaa
     5521 tatcaccggc tccgcatgcg gggccacttc cacctgttat cgagaaggag ggttcctcgg
     5581 ttgaacgaaa acagactgcg gttgcatggg ttcaacaatc tgaccaaggc gttgagtttc
     5641 aacatctacg acatctgcta cgccaagacc gaggcacaga agcgcgccta catcgagtac
     5701 atcgatgagc tctacaacgc cgaccggctg accaccatcc tcgaaggggt ggtcgagatc
     5761 atcggcgcca ccgtgctcaa tatttcgcga gaggattacg atccgcaggg ggccagtgtc
     5821 accatgctgg tggccgaccc gaccacaccg ccgtccgaac atctgatcac cgaggcgccc
     5881 ggtccgatgc cgcgcacagt gctgggccac ctggacaaga gccatctgac cgtgcacacc
     5941 tatccggaga gccatcccga cgaggggatc agcacctttc gcgcggacat cgatgtcgcc
     6001 acctgcgggc tgatctcacc gctgaaggcg ctgaactacc tgattcacgc ctttgaatcg
     6061 gacatcgtca cggtcgacta ccgggtacgc ggcttcaccc gcgatgtcga tggcgtcaag
     6121 cacttcatcg accacgagat caactcgatc cagaactact tctccgatga caccctcgac
     6181 cgctatcaga tgatcgatgt caatgtctat caggagaaca tctttcacac caagatgctg
     6241 ctcaaggagt tcaatctgga caactacctg ttcggtaccg gatcgagcga gctgagcgag
     6301 cgcgagcagg ccgagattcg caagcaactg catcgggaga tgcaagagat cttctacggt
     6361 cgcaacatct gctgagcaaa aaaaccgcca ttgtggccga ggccacaatg gcggaaacgc
     6421 actacactcc cactcaaatc aaatcggtcc cactcacctc agaagtcggc cgaggccatc
     6481 agccagagct tttcggtgtc gaacaggctg gcgttgacgg tgtcggcgct gtagttggca
     6541 tacttcagca tcaggctgta gtgcttgccg aactttctgg agatgaccag gtcgctttcg
     6601 ttgccatagt ccatgccgcc agtgtccgaa cggtagtcat gatagatata ggttagatca
     6661 acctgctcga tcttgccgcc gatgctgagg taacgatctt ccacgccatt ggccggggtg
     6721 gtcagaaagc ggtcggcata gccctggaac gcatgcagcg tggccagtgg ggtgctgaag
     6781 ccggtgccgt tgtcggagcc cagcagttca tagccgagtt tcacggtggc cagcggcagc
     6841 gccacgccgc cttcgaccag catgtagtca cgctcgaact catttgggtt gctgccgtgg
     6901 tccgactggc tggcatactc cagggcatag agcaacttgt agtcggtcag tggcttggcg
     6961 gtggccagac gcacaccata ggtggcgctc gacagcgcgg ccgaggccgt atcgtcgaag
     7021 tcgagcagat agccatagcc gctcagcttg ccgatcggca gcccttcgta gccgacattg
     7081 atcaggtgga cctcgccggg caggtcggcc aggttgacac catcatcggg gccgaaaatg
     7141 cggttgacgt tggtgatgta accgtagaag aggttgagct tgtcgatgct cgtgttggtc
     7201 agcgtgatgc cgtcgaaggt ctgttcgttc tgacgccagg cgacgccgcc gacgaagcgc
     7261 tggttgtcga ggttgatgcg ctgccggcca tatttgaggt cgtttttgcc aaacttgtag
     7321 gcgagccagg cctggttgac ctcggtggcg gtcggatcgg ccacgatcgg atagagcgtg
     7381 cggccattga cagtgctgtt gtaggtctca ccgccgatgg cgctgacatc atcgacctcg
     7441 atctgaccgg tgaagccctg ataggcggcg gtggtgaaat tgagtcgggt gcgcagcgtc
     7501 gaggcggccg cttcgcgtgc cttgttctgc tcatcgacgg tttcatagcg gtagcgcagg
     7561 ttgagcttgg cctgcccttg gctgatggcc tcggcaatcg aggtggcggc ggccgaactg
     7621 ctgctgccgt gcaggcagag cagcagtgcg gtcatgccgc tgaccagggt gcggatcacc
     7681 ggtgggttaa ggtgactttg ggtcatgaag atctccttga gggctgaaag aaaatcgcta
     7741 aaaaattcaa caaatccgcg ggttgcttat aaatcatcaa caacaattca gcattgatct
     7801 gtgtgcctgt tttgggcttg acaatcctgc gtcgcggcag acggtgttgc cgatcgactg
     7861 aggatggcgc gcagctccgg caggcaggag ccgcagttgg tgccggcacg cagccggttg
     7921 ccgatcacct ctacggtgca cagtcgctgg ctgtggatgg cctgttcgat ggtgttgagg
     7981 ccgacattga agcagacgca gatcgtgcgg ccagcatcgg gctgggccac ggccggacgg
     8041 gctgccagca gcgccgggcg ggctgacggc tcgatctggc cgcaggcgaa gcgctccagc
     8101 agccaggtgc gttcggcggg ctgcggtgtg cggtcgatga agatcaggct gtcgagccgt
     8161 ccgccaatga agcgcgcagc gcggtagcgg ccggcttcgg catcctggaa ctcgatccag
     8221 ctgctctcca gcatcggatc agtggcaggc agtcgtgcgg tgaaccagcc gcgccagtca
     8281 ttgatgggct gcttgccggc cagttcatgg cagtgtcctc cttcgatcgg gccgcgacac
     8341 cagtagtcga tctggtcggt ggtgaacggc tcgcggctga gcacgaatcc atgccaggtc
     8401 gcagcgaaag gctcgactgc aaccgcactc tgcttgaact ccggctggcc ggaaatcggg
     8461 tcggtgacgg cagccaccag gctgccgatg cgggcggccg aggcgaactg gtcgttccag
     8521 tgaatcggcg cgaacagttg accgcgttgc accgcgtcgc tgatctgtgc ccgcagcgtc
     8581 acgctgccgt gatgattgtg aacctggagc agcgcccgct caccaacgcc aagggtcttg
     8641 gcatcttctg ggtgaatctc gacaaagggc tcggcgatgt ggctgagcag acgcaccgag
     8701 cggccgctgc gagtcatggt gtgccactgg tcgcggatgc ggccggtgtt gagcagaaac
     8761 ggatggggtg catcgagtcg ggcgacggac aggtgtggcg tgatcggaat cagtctggcc
     8821 cggcccgacg gggtgtaata acggccatcg ctgaacagcc gggcgcagcc atcggggtgc
     8881 gtcggcgtga ccggccactg gatcggttgc agagcgtcat actgcgccgc gtcgagcatg
     8941 ctcaggccgc tgaggtcgaa gtcgcgctgg ccgtcgtttt caaagctgga gagccgcgca
     9001 tgttcacgga aaatctgctg gggtgtgtcg tagtcgaagg cgctcccgaa accgagcgcg
     9061 cgcgccacct gggtgaagat ccaccagtcg ggtctggcct cgcccggcag tggcagaaag
     9121 gcacgctggc gcgagatgcg gcgttcggag ttggtgacgg tgccatcctt ctcgccccag
     9181 gcggccgctg gcagcagcac atgggcatgt tgactggtgt cggtgcgccc ttcgcagtcg
     9241 gagacgatca gaaattcgca ctgttgcaac gcctgccgca cccggttggc ctcgggcagg
     9301 ctgacggcag ggttggtgcc ggcgatccag agcgcacgca cgcggccgtc ggccaccgcc
     9361 tggaacagct ccaccgcctt gagtcccggc tgatgcgcca gtgccggtgc atcccagaag
     9421 cggcgcaggc ggtcgaggtc ggccggattc tcatactcca tgtgcgccgc caaggtgttg
     9481 gccatgccgc ccacttcgcg gccgcccatc gcattgggct ggccggtgat cgagaacggt
     9541 gccgcgcccg gtttgccgat cttgccgctg gcgaggtggc agttgatgat ggcattgccc
     9601 ttgtcgacac cactggagga ttgattgatc ccctgcgaga agagcgtgac cacccgcgtg
     9661 gtgtcgccga accagtcgaa cagacgggca atggcggctt cgtcgacgcc acaggcggcg
     9721 gcggtgctgg cccggtcggg tgcgctggcc tgggcggcag ccagtgcggc ctcgaaacct
     9781 tcggtgtg
//
LOCUS       DAORCQ010000084         9729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4818_length_9729_cov_5.364689, whole genome shotgun sequence.
ACCESSION   DAORCQ010000084 DAORCQ010000000
VERSION     DAORCQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4818_length_9729_cov_5.364689"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKI23_08920"
     CDS             complement(<1..726)
                     /locus_tag="PKI23_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN37098.1"
                     /translation="MLGTMMQRPLSIPALLEHAARCHGDTEIVTRTTEGPIHRYGYAE
                     ALQRSKQLANALIRLGAHPNDRIATLAWNTHRHFELYYAISGIGAIVHTLNPRLFVDQ
                     LAYIINHAADRYLFVDLTFLPIVEQLAERIPTVLELIVLTDRAHMPTHSHLPRLHCYE
                     ELLAAENSQFNWPTMDENRAAALCYTSGTTGHPKGVLYSHRSTLLHALTVCQRQVLDL
                     SADTAVLPAVPMFHVCAWGMPYAA"
     gene            complement(795..1559)
                     /gene="moeB"
                     /locus_tag="PKI23_08925"
     CDS             complement(795..1559)
                     /gene="moeB"
                     /locus_tag="PKI23_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNN37099.1"
                     /translation="MDDQQLLRYSRQIMLPDFEIAGQERLLASRVLIVGLGGLGSAAA
                     LYLTAAGVGELRLADHDRVDLSNLQRQILHQNSDLGRPKVASAVDRLRALNPGVRLMP
                     IEQRLAADNLADWIDGVDLLLDCSDNFAIRFALNRASVTARIPLVSAAAIGYDGQLGS
                     FDPRQSDSPCYHCLYRDNGEDEAQGCARNGVLAPTVGVIGTLQAFEAIKLLTGIGETL
                     VGKLMLFDGRKMAWRTLRLPKDVACPVCASAPHFAG"
     gene            complement(1552..2400)
                     /gene="prmC"
                     /locus_tag="PKI23_08930"
     CDS             complement(1552..2400)
                     /gene="prmC"
                     /locus_tag="PKI23_08930"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014589668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNN37100.1"
                     /translation="MATLSALLRTACQRLDSSPTPELDARLLLCHLLGVGSSFLYGWP
                     EHPLSVTQQQQFEALIERRRQGEPIAYLTGRQGFWDLELRVDATTLIPRPETELLVEL
                     TLQRLPTTPLRLADLGTGSGAIALALAKARPAWQLVATDASPAALHVARDNAATLNLA
                     HRVCFQQGDWCDALPAGRFAALVSNPPYIAADDPHLAALGFEPATALVAAENGLADLR
                     RIAEQAPAHLLSGGWLLLEHGWQQGAAVRDLLSRLGYHQIETHHDLGGQERVTSARIG
                     EEHTDG"
     gene            complement(2431..3519)
                     /gene="prfA"
                     /locus_tag="PKI23_08935"
     CDS             complement(2431..3519)
                     /gene="prfA"
                     /locus_tag="PKI23_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNN37101.1"
                     /translation="MKPSIQHKLEQIQERQQELAALLSQAETIQDNSRFRELSREFSE
                     IEPVALCFGRYRQLLQQLDETRTLLDSSDAELRELAEAELGSDSAELERLETELQLLL
                     LPRDEHDNSNVYLEIRAGTGGDEAAIFAGDLFRMYSRHADLRGWRIEVVSERPGEHGG
                     FKEIVCHITGNQVYSHLKFESGTHRVQRVPATESQGRIHTSACTVAILPEVDEIDGIE
                     INKADLKVDTYRASGAGGQHVNKTDSAIRITHLPSGMVVECQEERSQHKNRAKAMSLL
                     AARLLDQARGKQQAEHAESRRLQVGSGDRSERIRTYNYPQGRITDHRINLTLYQLDEV
                     LGGHLDLLTMPLLHEYQAEQLARLADAH"
     gene            complement(3516..4841)
                     /gene="hemA"
                     /locus_tag="PKI23_08940"
     CDS             complement(3516..4841)
                     /gene="hemA"
                     /locus_tag="PKI23_08940"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNN37102.1"
                     /translation="MVDQSALPASDLALLALGINHTTADITLRERVAFNPARFGEALE
                     SARDSVPLREVAILSTCNRTELYCVPESLPGARQRLLAWLADYHHLPQKQLTQSLYTH
                     WGAKAARHMMRVAAGLDSMILGEPQILGQMKEAYAQAKAADTLGRRLNQVFQRTFAVA
                     KKVRTDTGIGQQSISVAYASVQLAKRIFTDLGRSHALLIGAGETIELVARHLREQGIG
                     SIQVANRTLERAEQLAREVGGTPILLSEIPQQLPQADLVISSTASPLPILGKGAVERA
                     LKARRHRPIFMVDLAVPRDIEAEVAELADVYLYTVDDLQEVVRENLRSRQGAADEAER
                     LITLGVEELMREERAHDAIELVKRYRGRAEQLQQAELEKARRLLAQGQDPQQVIERLA
                     HGLTNKLLHGPTVQIKAASELDDREQRLAIYRDLFSLECRRDHSDPTPR"
     gene            5125..6837
                     /locus_tag="PKI23_08945"
     CDS             5125..6837
                     /locus_tag="PKI23_08945"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN37103.1"
                     /translation="MLLAGCASKKVDLPQADAPALAAEVPAPAAAPAEPSRFRPFAKE
                     TLYDLLSAELAGKSQQYDLALDGYLQQARQTRDPVIIERALQIAQFLQNDAAMLEAAG
                     LLIEVDPANSEARRMYAIQLIRAERYLEALPQLDTLRATGQEAAYDYLALMAVRQPPE
                     RLDGLIVEFDRRLATAADDAQLLFGKAILLNARGQREAALQLAQQLSKMPQDNGKSMV
                     LEAQILFGMGREQQALQRLDEAVARFPDDLRLRVQLIQLLLSRQELARAGSEIDRFSA
                     LVDGNDGVLLSTVAAMAARSGLVSRAERLYQQLLDIDHRPDEAHFQLGLLAQQRNDFN
                     QAFYHFSAIGEGPLYLNAVAKRAELLAITGEFEAMQRLLADTREQWSSAAASLYLLEG
                     ELLMTHQRPGSAIKLYDRALKALPQDLNLRYGRAMAHEQLGDLVAMERDLRYIIKREP
                     DNAVALNALGYTLAVRTKRLSEAHRLILRAHQLRPQEPAILDSLGWVEHLRGNHAEAL
                     QLLEQAAQQLNEPEVIGHLVELLWQEGQQERARALLRKGMIDHPATPRWGELIKRLKI
                     DLEP"
     gene            6834..7445
                     /gene="lolB"
                     /locus_tag="PKI23_08950"
     CDS             6834..7445
                     /gene="lolB"
                     /locus_tag="PKI23_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNN37104.1"
                     /translation="MNLSLRTGLLMLLALWLGGCAQLARQPEPTPTGVQAAAEFLRRQ
                     ADLETMTHWQLAGKLGIRSGDQAVSGYFNWQEAPEHYLVQVSGPVGGGSMQIEGQPGR
                     VILRDSDGRSREAENVEMLLKSETGWWLPLSLLRYWIRGIPAPALPERHSLDPQGRIS
                     LLEQAGWKISYASYRQAGARELPEKIVLDTDHLHITLLLKAWQ"
     gene            7442..8287
                     /gene="ispE"
                     /locus_tag="PKI23_08955"
     CDS             7442..8287
                     /gene="ispE"
                     /locus_tag="PKI23_08955"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNN37105.1"
                     /translation="MSRLLTAPAPAKLNLFLHILGRRFDGYHRLQTLFQFLDHGDRLT
                     FEPIEDDQLELIAPGLPIPASDNLIMRAARLLRRDTGCRLGARIQLDKRLPMGGGLGG
                     GSSDAATTLVMLNRLWRLQLPPTELARLALELGADVPLFTLGRAAWGEGIGEQLTPVQ
                     LEEPWYLVLTPDCEVATGTIFQHPQLTRNSEPITMSAFLEGGGRNDFEPLVRKLYPQI
                     DAAFGWLGEFAPPRLTGSGSSLFARFATEETALRPLRQLPSGWRAFVARGCNTSPLYL
                     KSANE"
     gene            8342..8416
                     /locus_tag="PKI23_08960"
     tRNA            8342..8416
                     /locus_tag="PKI23_08960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8374..8376,aa:Gln,seq:ttg)
     gene            8482..9423
                     /locus_tag="PKI23_08965"
     CDS             8482..9423
                     /locus_tag="PKI23_08965"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNN37106.1"
                     /translation="MPPMKIFTGNATPELARSVAEILDMPLGSALVGSFSDGEILVEI
                     QENVRGDDVFIIQSTCAPTNDHLMELIIMVDALRRASASRITAVVPYFGYARQDRRVR
                     SARVPISAKVVADMMTGVGINRVLTVDLHADQVQGFFDVPVDNIYGSPVLIDDILRNN
                     FEDLLVVSPDIGGVVRARAFAKQLSSDLAIIDKRRPQANQTQVMNVIGEVEGRTCILV
                     DDIIDTAGTLCSAAAALKEQGAARVMAYCTHPVLSGRAIDNLDNSELDQLVVTDTIPL
                     SPAARQCSRVRQISLAPLLAEAVRRVSNEESISAMFR"
     gene            9512..>9729
                     /locus_tag="PKI23_08970"
     CDS             9512..>9729
                     /locus_tag="PKI23_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNN37107.1"
                     /translation="MSAQFELDAELRQQQGTGASRRLRRLENRVPAILYGGGKDPMLL
                     SCVAKDLAKSLESEAFYTHIINLKIGGS"
BASE COUNT         1596 a         3015 c         3361 g         1757 t
ORIGIN      
        1 cgcggcgtag ggcatccccc aggcgcagac atggaacatc ggcactgccg gcagcacggc
       61 ggtgtcggca gagaggtcga gcacctgccg ctgacagacc gtcaatgcat ggagcagcgt
      121 ggagcggtgg ctgtagagca cacccttggg gtgcccggtg gtgccggagg tgtagcagag
      181 ggccgccgca cggttctcgt ccatcgttgg ccagttgaac tggctgtttt cggccgccag
      241 cagctcttcg tagcagtgca ggcgcggcag gtggctgtgg gtcggcatgt gggcgcggtc
      301 ggtcagcaca atcagttcga gcacggtggg aatccgctcg gccaactgct cgacgatcgg
      361 cagaaaggtc aggtcgacga acaggtagcg gtcggcggca tggttgatga tgtaggccaa
      421 ctggtcgacg aacaggcgcg ggttgagggt gtggacgatg gcgccgatgc cggaaatggc
      481 gtagtagagt tcgaagtggc ggtgggtgtt ccaggcgagc gtggcgatgc ggtcattggg
      541 gtgggcgccg aggcggatca gcgcattggc caactgcttg ctgcgctgta gggcctcggc
      601 gtagccgtag cggtgaatcg gcccctcggt ggtgcgggtg acgatctcgg tgtcaccgtg
      661 gcagcgggcc gcatgttcga gcagggcagg aatcgacagc ggacgttgca tcatggtgcc
      721 cagcatggcg tgctcctctt tcttgttgtt gtgatgggcg gtgcgcggtc ggaggcacta
      781 tagaggcttg ccactcaccc ggcaaagtgt ggcgcgctgg cgcagaccgg gcaggcgaca
      841 tccttcggca gccgcagcgt tcgccaggcc atcttgcgtc catcgaacag catcagcttg
      901 ccgaccaggg tctcgccgat gccggtcagc agcttgatcg cctcgaaggc ctgcaatgtg
      961 ccgatcaccc cgaccgtcgg ggccagtacg ccattgcggg cgcagccctg ggcttcatcc
     1021 tcgccgttgt cgcggtagag gcagtggtag caggggctgt cgctctggcg cggatcgaag
     1081 ctgccgagct ggccgtcgta accgatcgcc gcggccgaca ccagcggaat gcgggctgtg
     1141 acgctggcgc ggttgagggc gaagcggatg gcgaagttgt cgctgcaatc gagcagcagg
     1201 tcgacgccgt cgatccagtc ggccaggttg tcggcagcca gtcgctgttc gatcggcatc
     1261 agccgcacgc ccggattgag cgcccgcagc cggtcgaccg ccgaggccac cttgggacgg
     1321 ccgaggtcgc tgttctgatg cagaatctgc cgttgcaggt tggagagatc gacccggtcg
     1381 tggtcggcca gccgcagttc gccgacgccg gcggccgtca gatagagggc ggccgcgctg
     1441 ccgagcccgc cgagcccgac gatcagcacc cggctggcga gcagtcgctc ctgcccggcg
     1501 atctcgaagt cgggcagcat gatctgtcgg ctgtagcgca gcagttgctg gtcatccatc
     1561 ggtgtgctcc tcgccgatgc gggcagaggt gacccgctcc tgtccgccga gatcgtggtg
     1621 ggtctctatt tgatggtaac cgagtcggct cagcaggtcg cgcaccgcgg cgccctgttg
     1681 ccagccatgc tccagcagca gccagccacc gctcagcagg tgggcggggg cctgctcggc
     1741 gattcggcgc agatcggcca ggccattctc ggccgccacc agcgccgtgg ccggttcgaa
     1801 accgagcgcg gcgagatggg ggtcatccgc ggcgatgtag ggggggttgc tgacgagggc
     1861 ggcaaagcgg ccggccggca gtgcgtcgca ccagtcgccc tgctggaagc agacacggtg
     1921 cgcgagattc agggttgcgg cattgtcccg cgccacatgc agcgcggccg ggctggcatc
     1981 ggtggcgacc agctgccagg ctgggcgcgc cttggccagt gccagcgcga tggcgccgct
     2041 gccggtgccg agatcggcca gccgcagcgg ggtggtcggc agccgttgca gcgtcagctc
     2101 gaccagcagc tcggtctcgg gccgggggat cagggtggtg gcgtcgaccc gcagctccag
     2161 atcccagaaa ccctgccggc cggtcaggta ggcgatcggc tcgccctgtc ggcgccgctc
     2221 gatcagcgct tcgaactgct gctgttgcgt gacactgagc gggtgctccg gccagccata
     2281 gagaaagctg ctgccgacac cgagcaggtg gcacagcagc aggcgggcat ccagttccgg
     2341 tgttggactg ctgtcgagtc gttggcaggc agtgcgcagc agggcagaca gggtggccat
     2401 cgcgatcagc cagcggttgc cggcagcgcc tcagtgggca tcggccaggc gggcgagctg
     2461 ttcggcctgg tactcatgca gcagcggcat ggtcagcaga tcgagatggc cgcccagcac
     2521 ctcgtcgagc tgatagagag tgaggttgat gcggtggtcg gtgatgcgtc cctgtgggta
     2581 gttgtaggtg cgaatccgct cggagcggtc gccggagccc acctgcagcc gccgcgattc
     2641 ggcatgttcg gcctgctgct tgccgcgggc ctgatcgagc agccgggcgg ccagcagcga
     2701 catcgccttg gcgcggtttt tgtgctgcga gcgctcctcc tgacactcca ccaccatgcc
     2761 gctcggaagg tgggtgatgc ggatcgccga atcggtcttg ttgacatgct gccctcccgc
     2821 ccccgaggcg cggtaggtgt cgaccttcag atcggccttg ttgatctcga tgccgtcgat
     2881 ttcgtcgacc tccggcagga tggcgacagt gcaggccgag gtgtggatgc gtccctgtga
     2941 ttcagtggcg ggcacccgct gcacccggtg ggtgcccgat tcgaacttga ggtgggagta
     3001 gacctggttg ccggtgatgt gacagacgat ctccttgaag ccgccgtgct cgccgggccg
     3061 ttcgctgacc acctcgatgc gccagccgcg caggtcggca tggcggctgt acatgcgaaa
     3121 caggtcgccg gcgaagatcg cggcctcatc accgccggtg ccggcacgaa tctccaggta
     3181 gacgttgctg ttgtcatgct catcgcgcgg cagcagcagc agttgcagct cggtttcgag
     3241 ccgttcgagt tcggcgctgt cgctgccgag ctcggcttcg gccagttcgc gcagttcggc
     3301 gtcgctgctg tcgagcagtg tgcgggtctc atcgagctgc tgaagcaact gccggtagcg
     3361 gccaaagcag agggcgaccg gttcgatctc gctgaactcg cgcgacagct cacgaaagcg
     3421 gctgttgtcc tggatggtct cggcctggct cagcagggcg gccagctcct gctggcgctc
     3481 ctgaatctgc tccagcttgt gctgtatcga gggtttcatc gaggggtcgg gtcgctgtgg
     3541 tcgcgacggc attcgaggct gaagaggtcg cggtagatcg ccagccgctg ctcgcggtca
     3601 tcgagttcgc tggcggcctt gatctgcacc gtggggccat gcagcagctt gttggtcagg
     3661 ccgtgcgcca gccgctcgat cacctgctgc ggatcctgcc cctgcgccag cagtcggcgg
     3721 gccttctcca gctcggcctg ctggagctgt tcggcccggc cgcggtagcg cttgaccagt
     3781 tcgatggcgt cgtgggcgcg ctcttcgcgc atcagctcct cgacccccag cgtgatcagc
     3841 cgttcggcct cgtcggcggc gccctggcgg ctgcgcaggt tctcgcgcac cacctcctgc
     3901 aggtcgtcga cggtgtagag gtagacatcg gccagttcgg ccacctcggc ctcgatgtcg
     3961 cgcggcacgg cgaggtcgac catgaagatc ggccggtggc ggcgtgcctt cagtgcccgc
     4021 tcgacggcgc ccttgccgag gatcggcagc gggctggcgg tggaggagat caccagatcg
     4081 gcctgtggca gttgttgcgg aatttcgctc agcaggatcg gggtaccgcc gacctcacgc
     4141 gccaactgtt cggcccgctc cagcgtgcgg ttggccacct gaatcgagcc gatgccctgt
     4201 tcacgcaggt ggcgcgccac cagctcgatg gtctcgccgg cgccgatcag cagggcatgg
     4261 ctgcggccga ggtcggtgaa gattcgcttg gccagttgca ccgaggcgta ggcgaccgag
     4321 atcgactgct ggccgatgcc ggtgtcggtg cgcaccttct tggccaccgc aaaggtgcgc
     4381 tggaacacct ggttgagccg gcggcccagc gtgtcggccg ccttggcctg ggcgtaggcc
     4441 tccttcatct gccccagaat ctgcggctca ccgagaatca tcgagtcgag tccggcggcc
     4501 acccgcatca tgtggcgcgc cgccttggcg ccccagtggg tgtagaggct ctgggtcaac
     4561 tgcttctgtg gcaggtggtg gtagtcggcg agccaggcga gcagccgctg gcgggcgccc
     4621 ggcagcgatt cgggcacgca gtagagttcg gtgcggttgc aggtcgacag aatcgccacc
     4681 tccctgagcg gcacactgtc gcgcgcactt tccagtgcct cgccgaagcg tgccgggttg
     4741 aaggcgaccc gttcgcgcag agtgatgtcg gccgtggtgt gattgatgcc gagcgccagc
     4801 agtgcaaggt cggaagcggg gagggcggac tggtcaacca tggtctgtca gtgataaccc
     4861 agttgtggtg cgcatcgtga gtgagaaagt gaggcgtgct gccccgggtt ccatggattc
     4921 acggacaaca gcacaggccg ccgtggccgg atctgttgcg gcttgccgat cctgtggcgg
     4981 aaatatacca caaaagcacg gggttaccgg cagttgcgcc gtgccccatt gtggcccatg
     5041 cttcgatgcg atttaatgcg acatccactc gaacagccct tcatgccatc cacccgtcct
     5101 cttgctgcct gcattgtcct gttgatgctg cttgctggct gcgccagcaa aaaagtcgac
     5161 ctgccccagg ccgatgcgcc agctctggcg gccgaggtgc cggcgccggc ggctgcaccg
     5221 gccgaaccgt cgcggtttcg cccctttgcc aaggagacgc tctacgacct gctgagtgcc
     5281 gagctggccg gcaagtcgca acagtacgat ctggcgctcg atggctacct gcagcaggcg
     5341 cggcagacgc gtgatccggt catcatcgag cgggccctgc agatcgccca gttcctgcag
     5401 aatgacgcgg ccatgctcga agcggccggg ctgctgatcg aggtcgatcc agccaattcc
     5461 gaagcgcggc ggatgtatgc cattcaactg atcagggccg agcgttacct cgaggcgctg
     5521 ccgcaactgg acaccctgcg cgcgaccggg caggaggctg cctacgacta tctggcattg
     5581 atggccgttc gccaaccgcc ggagcggctc gatggcctga ttgtcgagtt cgatcggcgg
     5641 ctggcgacgg ccgcggatga tgcgcaactg ctgttcggca aggcaatcct gctcaatgcg
     5701 cgtggtcagc gcgaggcggc cctgcaactg gcgcagcagt tgtcgaagat gccgcaggac
     5761 aatggcaaga gcatggtgct ggaggcgcag atcctgttcg gcatggggcg cgaacagcag
     5821 gccctgcagc gactggacga agcagtggcg cgcttccccg acgacctgcg gttgcgggtg
     5881 cagttgattc aactgctgct gagccggcag gagctggctc gggccggcag cgaaatcgac
     5941 cggttcagcg cgctggtcga cggcaacgat ggtgtgctgc tgtcgacggt ggccgccatg
     6001 gcggcgcgca gcggcctggt cagtcgggcc gagcgactct atcagcagct gctcgacatc
     6061 gaccaccgtc ctgatgaggc ccactttcaa ctgggtctgc tggcacagca gcgcaacgat
     6121 ttcaatcagg cgttctatca cttttcggcg atcggcgaag gcccgctcta tctcaacgcg
     6181 gtggccaagc gggcggagct gctggcaatc accggtgaat tcgaggcgat gcagcggctg
     6241 ctggccgaca cccgtgagca gtggagttcc gccgcggcca gcctctacct gctcgaagga
     6301 gagctgctga tgacccatca gcggcccggc agtgcgatca agctctacga tcgggcgctg
     6361 aaggcgctgc cgcaggatct caatctgcgc tatggccgtg cgatggccca tgaacagttg
     6421 ggcgatctgg tggcgatgga gcgcgatctt cgctacatca tcaagcgcga accggacaat
     6481 gccgtggcac tgaatgcgct gggctacacc ctggcggtgc gcaccaagcg tctgagcgaa
     6541 gcccatcgac tgattctgcg ggcccaccag ttgcggccgc aggagccggc gattctcgac
     6601 agcctcggct gggtcgaaca tctgcgcggc aaccatgccg aagcgctgca gttgctggag
     6661 caggccgccc agcagctcaa cgagccggag gtgatcggcc atctggttga gctgctgtgg
     6721 caagagggcc agcaggagcg ggcgcgggca ctgctgcgca aggggatgat cgaccatccg
     6781 gccactccgc gctggggtga actgatcaag cgcctgaaga tcgatctcga accatgaacc
     6841 tgtcgttgcg caccggcctg ttgatgcttc tggcgctgtg gctggggggc tgtgcccaac
     6901 tggccaggca gccggagccg acgccaaccg gcgtgcaggc cgcggcggaa tttctcaggc
     6961 gccaggcgga tctggagacg atgacgcact ggcagttggc cggcaaactg ggcattcgca
     7021 gcggtgacca ggcggtcagc ggctatttca actggcagga ggcaccggag cactatctgg
     7081 tgcaggtgag cggtccggtg ggtggcggct cgatgcagat cgaaggacag ccgggtcggg
     7141 tcatcctgcg cgacagcgac ggcagatcgc gtgaggccga gaacgtcgag atgctgctga
     7201 agagtgaaac cggctggtgg ctgccactgt cgctgctgcg ctactggatt cgcggcattc
     7261 cggctccggc actgcccgag cgccacagcc tcgatccaca ggggcgcatc agcctgctcg
     7321 aacaggccgg ctggaaaatc agctatgcca gttatcgaca ggcgggggcg cgcgaactgc
     7381 cggagaagat cgtgctcgac accgaccatc tgcacatcac gctgctgctc aaggcgtggc
     7441 aatgagcagg ctattgaccg cgccggcgcc ggccaagctc aacctgtttc tgcacattct
     7501 ggggcgccgc ttcgacggct atcaccggtt gcagacactg ttccagttcc tcgaccatgg
     7561 tgatcggctc accttcgagc cgatcgagga tgatcagctc gaactgattg ctccggggct
     7621 gccgattccg gcatcggaca acctgatcat gcgggcggca cggctgctca ggcgtgacac
     7681 cggctgccgt ctgggcgcgc gcatccagct cgataagcgg ttgccgatgg gcggtggcct
     7741 gggcggcggc agctccgacg cggcgaccac gctggtgatg ctgaatcgac tgtggcggct
     7801 gcaactgccg ccgaccgagc tggcccggct ggcgctcgaa ctgggtgccg atgtgccgct
     7861 gttcacgctg gggcgggcgg cctggggcga gggcatcggt gagcagttga cgccggtgca
     7921 gctcgaagag ccctggtacc tggtgctcac gcccgattgc gaagtggcaa ccggtacgat
     7981 ctttcagcat ccgcaattga cacgcaacag tgagccgatc acaatgtcgg cctttttgga
     8041 ggggggcggt cgcaacgatt tcgagccgct ggttcgcaaa ctctacccgc agatcgatgc
     8101 cgccttcggc tggctcggag agtttgcccc gccgcggttg accggttcgg gcagctccct
     8161 gtttgcccgg tttgcaaccg aagagactgc gttgcggccg ttgcggcagt tgcccagtgg
     8221 ctggcgggct ttcgtcgccc ggggctgcaa cacctctcca ctgtatttga agagtgcaaa
     8281 cgaatgaagc ggcgtcacgg cacagcccgg accgcagtac cattcgcagg tttttaacca
     8341 ttggggtgtc gccaagcggt aaggcactgg attttgattc cagcatacga aggttcgaat
     8401 ccttccaccc cagccatctt tccgtgcgtc atacctgaaa tcgtccacct ttactgacgc
     8461 cgatcagcct gaggtttcgt gattccgccc atgaaaatct tcactggcaa tgccaccccg
     8521 gagcttgccc gcagtgtcgc cgagattctc gacatgccgc tgggcagtgc gctggtcggc
     8581 agcttcagcg atggcgaaat cctggtcgag attcaggaga atgtgcgcgg tgatgatgtc
     8641 ttcatcatcc agtcgacctg cgcgccgacc aacgaccacc tgatggaact gatcatcatg
     8701 gtcgatgcgc tgcggcgcgc ctcggccagc cggatcaccg cagtggtgcc ctacttcggc
     8761 tatgcccgac aggatcgccg ggtgcgctcg gcgcgggtgc cgatcagcgc caaggtggtg
     8821 gccgacatga tgaccggcgt tggcatcaac cgggtgctca ccgtcgatct gcatgccgac
     8881 caggtgcagg gctttttcga cgtgccggtg gacaacatct atggctcgcc ggtgctgatc
     8941 gacgacatcc tgcgtaacaa tttcgaagac ctcctggtgg tttcgcccga tatcggtggc
     9001 gtcgtgcgtg cacgcgcctt tgccaagcag ctcagcagtg atctggcaat catcgacaag
     9061 cgccgccccc aggccaacca gacgcaggtg atgaacgtga tcggcgaggt cgaggggcgc
     9121 acctgcattt tggtcgacga catcatcgac accgccggta ccctctgcag tgccgcggcg
     9181 gccctgaaag agcagggcgc ggcccgggtg atggcctact gcacccaccc ggtgctgtcg
     9241 ggacgcgcca tcgacaacct cgacaactct gagctcgatc aactggtggt gaccgacacc
     9301 atcccgttgt cgccggctgc ccggcagtgc agccgagtgc ggcagatctc gctggcaccg
     9361 ctgctggccg aagccgtgcg ccgggtcagc aacgaggagt cgatcagcgc catgttccgc
     9421 tgatcgacct tttcacaacc gatttcaacc acccaccggt cggcgtcgtt ttggtcgcga
     9481 aacccgtcga ctgtcagcaa aggagcccgc gatgagcgct caatttgaac tggacgccga
     9541 acttcggcaa cagcagggga caggtgcgag ccgccgcctg cgtcgtctcg aaaaccgggt
     9601 gcctgccatc ctctatggcg gcggcaagga tccgatgctg ctcagctgcg tggccaagga
     9661 tctggcaaaa tcgctggaga gcgaggcctt ctacacgcac atcatcaacc tcaagatcgg
     9721 tggcagcag
//
LOCUS       DAORCQ010000085         9691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4844_length_9691_cov_5.286011, whole genome shotgun sequence.
ACCESSION   DAORCQ010000085 DAORCQ010000000
VERSION     DAORCQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4844_length_9691_cov_5.286011"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKI23_08975"
     CDS             complement(<1..345)
                     /locus_tag="PKI23_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37108.1"
                     /translation="MSSSINPILEAEGTQAPLRIVKIRRDYNTWVANETLEDYALRFT
                     PHAFRKWSIFRVSNTAFGAISFLVLEAIGGTLVLNYGFTNTLWATLAVSLIIFLTALP
                     IGHYAARYGLDMD"
     gene            complement(451..1140)
                     /gene="urtE"
                     /locus_tag="PKI23_08980"
     CDS             complement(451..1140)
                     /gene="urtE"
                     /locus_tag="PKI23_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020294146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNN37109.1"
                     /translation="MFEVENLEVSYGQSRVIHGISFRAEKNETLAIMGRNGMGKTTLF
                     KSLMGILPSSSSKAVVDGENLQGIESHQRVARGLAYVPQGRMIFPGLTVEENIQTGLE
                     KSNSRRIPDEIFALFPVLFDMRHRKGGNLSGGQQQQLAIARALVTEPKVLLLDEPTEG
                     IQPSIIKEIARVLNEIRRLREITIIVSEQVLSFTLEVADRIIVIDKGRFIHEDRRDQV
                     DAARIKGYLSV"
     gene            complement(1187..1930)
                     /gene="urtD"
                     /locus_tag="PKI23_08985"
     CDS             complement(1187..1930)
                     /gene="urtD"
                     /locus_tag="PKI23_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004886694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNN37110.1"
                     /translation="MSNTDFVLAVEDLTVSFDGFKAVDALTMYVDKNELRVVIGPNGA
                     GKTTLLDLICGKTRSTSGSIKFNNQELTKLSEHEIVRSGVGRKFQTPSIYENLTVYQN
                     LEVSFPQGRTVFGSLFFRRSAEVARRVRSVAEEIFLDEQLEMEAALLSHGQKQWLEIG
                     MLLMQEPELLMLDEPVAGMSAKERDQTADLLKRICRNRSVIVIEHDMEFVERIAHKVT
                     VLHQGRILAEGTMEKVQADDRVIEVYLGH"
     gene            complement(1927..3084)
                     /gene="urtC"
                     /locus_tag="PKI23_08990"
     CDS             complement(1927..3084)
                     /gene="urtC"
                     /locus_tag="PKI23_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004886696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNN37111.1"
                     /translation="MTSYFSRLLGGREGAIGLVLLAALLLMVLPLSLDLFRLGLVGKY
                     ITYAFVAVSLVLLWGNGGILSLGQGVFFGLGGYCMAMFLKLEASDPVSTAIQSTPGIP
                     DFMDWNQLTALPWFWEPFKSLSFTIVAILLVPTLFAFVIGFAMFKRRVGGVYFAIITQ
                     AVALILSILIVGQQGYTGGVNGITDLKTLHGWDIRTDSAKYILYYVSALLLLGCILLS
                     RGILGSKFGMLLLAMRDKEERVRFSGYDVSDFKIFIFCVSAMISAIGGAMFTLQVGFM
                     SPSFVGIVPSIEMVIFAAVGGRMSLIGAVYGTLLVNFGKTMFSETYPELWLFLMGGLF
                     IAVVMFFPNGLAGIYQKYAKRSGLLRRMMQPATNGSCARISSESKKMGART"
     gene            complement(3098..4015)
                     /gene="urtB"
                     /locus_tag="PKI23_08995"
     CDS             complement(3098..4015)
                     /gene="urtB"
                     /locus_tag="PKI23_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNN37112.1"
                     /translation="MFEYSMAEIGNIVVMQGFSGLSLFSVLLLMALGLAIIFGQMGVI
                     NMAHGEFMALGAYTTVLFSRFAVSHELVSHYFVFAIAAAFVVAFVVGYLIEYGMIRHL
                     YKRPLDTLLATWGLSLVMQQCFRSGFGAKEVSATLPEWLLGSFKPTPDIDIPINGVFV
                     MVLALVVTVGVGLFMFRSRWGLRMRATVQNRVMADAVGINTRKVDRVTFALGCGIAGV
                     AGAAFTTIASTGPTTGSLYIVDSFMVVVFGGAASLLGTITSAFGIAQAQSILQFFMTS
                     SMGKVATLMVIVGILMLRPEGLFASKLRK"
     gene            complement(4212..5456)
                     /gene="urtA"
                     /locus_tag="PKI23_09000"
     CDS             complement(4212..5456)
                     /gene="urtA"
                     /locus_tag="PKI23_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HNN37113.1"
                     /translation="MKKLNRGRWVEGAATVTRSLLAAGVLIGSALASAPPTAQVNTTG
                     LAVTDSSVKVGILHSATGTMAISETGSIQAEKLAIAQINAMGGVLGRKIEVIQEDGAS
                     DWPTFAEKSKKLLVNDKVAAVFGCWTSASRKAALPVFEKENGLLFYPTFYEGLEQSKN
                     VFYTGQEATQQILAGLDWIAKEKGAKSFYLIGSDYIWPRTSMKIARKHIEQKLGGKVV
                     GEEYIALGDTQFGSVINKIKLKKPDVIYAAVVGGSNVAWFKQLNAAGLNSGKQTMLTI
                     SVTEDEVLGIGGENLAGFYSAMKYFQSLKNPNNEQFVAEFKKMWGPNSVIGDVTQAAY
                     LGPWLWKAAVERAGSFDVDKAAAALPGYEMTTAPEGYVKVESNHHLSSKLRIGQWRAD
                     GQADVVYESDLIAPDPFPKGYQ"
     gene            complement(5514..5864)
                     /locus_tag="PKI23_09005"
     CDS             complement(5514..5864)
                     /locus_tag="PKI23_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNN37114.1"
                     /translation="MPIYEYRCEQCGPFTALRRLSEFEQPAHCDGCGALSARIPSLPR
                     LALVGHAVRVAHERNERSVHEPAQLRRSVCGCSGVHHCQSTRGKPAAAATVQGGTPLQ
                     GQTRKTARPWMLGH"
     gene            complement(5878..7101)
                     /locus_tag="PKI23_09010"
     CDS             complement(5878..7101)
                     /locus_tag="PKI23_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HNN37115.1"
                     /translation="MKTLVKLDLDKKPWEQDGQIHNRWHCDLPMIAMVKPGDEFRVEC
                     MDWTGGQIGNNDSANDIRDVDLLQVHYLSGPIGVEGAEPGDLMVVDILDVGTFDHSQW
                     GFNALFSRQNGGGFLTDHFPEARKSIWDFHGIYTTSRHVPNVKFAGIMHPGLIGCLPS
                     RALLENWNRREAALIATDPNRVPALALPPDAQAAVMGRMQGDAAKSAAGEAARTVPPR
                     DHGGNCDIKNLTKGSRVYFPVYVKDGGLSMGDLHFSQGDGEITFCGAIEMAGYLDIKV
                     SLIKEGVKKYGIKNPLFQPSPLTPAYRDYLIFEGISVDEHNTQHYLDVTVAYRQACLN
                     AIEYLKKFGYSGAQALSILGTAPVEGHISGVVDIPNACATLWLPTEIFDFDLKPNADG
                     PQKRVLPGIDMAVAS"
     gene            complement(7314..9572)
                     /gene="relA"
                     /locus_tag="PKI23_09015"
     CDS             complement(7314..9572)
                     /gene="relA"
                     /locus_tag="PKI23_09015"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017527554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HNN37116.1"
                     /translation="MVKVRDDHPITAEGEIDLQSWCASLEQFVPQLVREPVLQAARWV
                     QQIEQRDPSSQEYRDHSYDCLQMGLEMARIVAELHLDQDSIIAATLYRAVRRGKLPLE
                     QVKQEFGSKIAKLIAGVQSMAAISAGSANNDSVFGKNQDQVKNIRAMLVAVIDDVRVL
                     LLKLAERTCAIRAVKGLDPVRQQRVSREVFDFYVPMAHRLGVGHIEWELEDLSFRYLE
                     PDAYQSIARLLDERRRDRDDYIQRVIQQIKQEMQSAGVAVQVYGRSKHIYSIWRKMQK
                     KGLDFSQIYDVRAVRVLVPTVRDCYTTLGVVHSLWKHIPNEFDDYIANPKENGYRSLH
                     TAVIGPQGRVVEVQIRTEEMHEEAEYGVCAHWLYKDPGKARKSKDYEEKIAWLQQALE
                     WHEEMGGLGNIAEHLRSYERQERIYVFTPDDHVVSLPHNATPVDFAYHVHTEVGNSCR
                     GAKVNGRIVPLNHPLQTGDRVEILRGPHSRPSRDWLNSSLQYIRTARARAKVQQWFRQ
                     QNREQNILDGRNLLEKEFQRLAITGIEPQQLADEVNLKSIDDVHADLGSGRLRLSQLL
                     NAAQRIENRTAQRAQMELLPTTLARAARSEATLAKDAVAIQGVGNLLTQFASCCKPVP
                     GDAITGFITRGRGVSIHRADCNNILQLEDNEPGRIIAVDWSGRTAQIYPVDILIEAYD
                     RSGLLRDITTVLARDSINVIAVNTLSHKESGTATMRLTIEVTSFDELGKVLARINQLS
                     NVIEARRFTAPA"
BASE COUNT         1885 a         3095 c         2851 g         1860 t
ORIGIN      
        1 atccatgtcc agcccgtaac gggccgcgta gtgaccgatc ggcagagcgg tcagaaagat
       61 gatcaggctg accgccagcg tggcccacag ggtgttggtg aagccgtagt tgagcaccag
      121 ggtgccgccg atggcttcga gcaccagaaa cgaaatcgcg ccgaaggcgg tgttggagac
      181 gcgaaagatc gaccacttgc gaaatgcatg cggtgtgaag cgcagggcat aatcttccag
      241 cgtctcgttg gcgacccagg tgttgtaatc acggcggatc ttgacgatgc gcagtggcgc
      301 ctgcgtaccc tctgcctcaa gaatcggatt gatactgctc gacatcgctg cccattcatc
      361 cgttgcctgc acggctcagg caagattcat gcgcaaacca tgcagcaggt cacgctggcg
      421 cagctcaatc gacgacattc gtcactccag tcagaccgag agatagccct tgattcgggc
      481 ggcatcgacc tgatcgcgcc ggtcttcatg gatgaagcgg cccttgtcga tgacgatgat
      541 gcgatcggcg acctccagcg tgaagctcaa cacctgctcg gaaacgatga tggtgatctc
      601 gcgcagccgg cgaatctcgt tgagcaccct ggcgatctcc ttgatgatcg acggctgaat
      661 gccctcggtt ggttcatcca gcagcaaaac cttgggttcg gtgaccaatg cccgggcaat
      721 cgccagttgc tgctgctgtc cgccggagag gttgcccccc ttgcgatgac gcatgtcgaa
      781 cagcaccgga aacagcgcga agatctcatc gggaatccgg cggctgttcg atttctccag
      841 accggtctgg atgttctctt cgacggtcag ccccggaaag atcatccgac cctgcggcac
      901 ataggccagc ccccgggcaa cacgttgatg gctctcgatg ccctgcagat tctcgccatc
      961 gaccactgcc ttgctgctgg agctgggcag aatgcccatc agcgacttga acagcgtggt
     1021 cttgcccatg ccgttgcggc ccatgatggc gagggtttcg tttttttccg ccctgaaact
     1081 gatgccgtgg atgacacgac tctgaccgta gctgacctca aggttttcga cttcgaacat
     1141 gtgacgctcc tgaattgaat gctgaaaccc atcgacatat cgcgcctcag tggccgagat
     1201 agacctcgat caccctgtcg tcggcctgca ccttctccat cgtgccttcg gcgagaatcc
     1261 ggccctgatg cagcaccgtc accttgtggg cgattctctc gacgaactcc atgtcatgtt
     1321 cgatgacgat caccgaacgg ttccggcaga tgcgcttgag cagatcggca gtctggtccc
     1381 gctccttggc gctcatgccg gccaccggct cgtcgagcat cagcagctcc ggctcctgca
     1441 tcaacagcat gccgatctcc agccactgct tctggccatg gctcagcagg gccgcctcca
     1501 tctccagttg ctcatccaga aagatctcct ccgccacact gcgcacccgc ctggccacct
     1561 cggcactgcg tctgaagaac agactgccga acaccgtgcg cccctgggga aaggagacct
     1621 cgaggttctg gtagaccgtc aggttttcgt agatcgatgg cgtctggaat ttgcgtccga
     1681 caccggaccg gacgatctca tgctccgaca gcttggtcag ctcctggttg ttgaacttga
     1741 tcgatcccga ggtcgaccgg gtcttgccgc agatcaggtc gagcagcgtg gtcttgccgg
     1801 cgccgttggg accgatcacc acccgcagct cgttcttgtc gacatacatc gtcagcgcgt
     1861 cgacagcctt gaagccatcg aaggagaccg tcagatcctc gaccgccagc acaaagtcgg
     1921 tattgctcat gtccttgccc ccattttttt cgattcgctc gaaattcttg cgcaggagcc
     1981 gttcgtcgcc ggctgcatca tccgccgcaa caggccgctt cttttggcat atttctgata
     2041 gatgcccgcc agtccgttgg gaaagaacat caccaccgcg atgaacagcc cacccatcag
     2101 gaacagccag agctcgggat aggtctccga gaacatggtc ttgccgaagt tcaccagcag
     2161 cgtgccgtag accgcgccga tcaacgacat gcggccaccc acggcggcga agatcaccat
     2221 ctcgatcgaa ggcacgatgc cgacgaacga gggcgacatg aatccgacct gcagcgtgaa
     2281 cattgcgcca ccgatggccg agatcatcgc cgagacacag aagatgaaga tcttgaaatc
     2341 ggagacgtca tagcccgaaa agcgcacccg ctcctccttg tcgcgcatcg ccagcagcag
     2401 catgccgaac ttgctgccga gaatgccgcg cgacagcagg atgcagccga gcagcagcag
     2461 tgcgctgacg taatagagga tgtacttggc actgtcggtc cggatgtccc agccatgcag
     2521 ggtcttcaga tcggtgatgc cattgacgcc gccggtgtaa ccctgctggc cgacgatcag
     2581 aatgctgagg atcagcgcca ccgcctgggt gatgatggcg aagtagaccc cccccacccg
     2641 gcgcttgaac atggcgaagc cgatcacaaa ggcgaacagc gtcggcacca gcagaatcgc
     2701 cacgatcgtg aacgacagac tcttgaacgg ctcccagaac cagggcagcg cggtcaactg
     2761 gttccagtcc atgaagtcgg ggatgcccgg cgtgctctga atcgcagtgc tgaccgggtc
     2821 ggacgcctcc agcttcagga acatcgccat gcagtagccg cccagcccga agaacacccc
     2881 ctgccccaga ctgagaatgc cgccattgcc ccacagcagc accaggctga ccgcgacaaa
     2941 ggcataggtg atgtacttgc cgaccaggcc aagacgaaac agatcgagtg acagcggcag
     3001 caccatcagc agcagcgccg ccagcaggac caggccaatg gccccctctc ggccaccgag
     3061 caatcgactg aaatatgatg tcatgatccg attcctccta tttgcgcagc ttggatgcaa
     3121 acagcccttc cgggcgcaac atcagaatgc cgacgatgac catcagggtg gccaccttgc
     3181 ccatcgaact ggtcatgaag aactgcagga tcgactgcgc ctgggcaatg ccgaacgccg
     3241 aagtgatggt gccgagcaga ctggcggcgc cgccgaacac caccaccatg aagctgtcga
     3301 cgatgtagag cgagccagtg gtcggcccgg tcgaggcgat ggtggtgaag gcagcacccg
     3361 ccacgccggc aatgccgcaa cccagcgcga aggtgacccg atcgaccttg cgggtgttga
     3421 tgcccacggc atccgccatg acccggttct gcaccgtggc ccgcatccgc agcccccagc
     3481 gtgagcgaaa catgaacaga ccgacaccca cggtgaccac cagtgccagc accatgacga
     3541 agactccgtt gatcggaatg tcgatgtcgg gggtcggctt gaacgatccg agcagccact
     3601 ccggcagggt ggcactcacc tccttggcgc caaaaccgga acggaagcac tgctgcatca
     3661 ccagactgag cccccaggtg gcgagcaggg tgtcgagcgg ccgcttgtag aggtgccgga
     3721 tcattccata ctcgatcagg taacccacca cgaaggcgac cacgaaggcc gcggcgatcg
     3781 caaagacgaa gtagtggctc accagttcat ggctgacggc gaagcgggaa aagagcaccg
     3841 tggtgtaggc ccccagcgcc atgaactcgc catgcgccat gttgatcacg cccatctggc
     3901 caaagatgat cgccagaccc agcgccatca gcagcagcac gctgaacaga ctgagaccgg
     3961 aaaaaccctg catcaccacg atgttgccga tttcggccat cgaatactca aacatggtcg
     4021 aacctccgga tgggtgcacc ctgccctttg atgcgcatcc ggccgacggc aggacgcggc
     4081 ggtcgaatcg atcgagttgc ctggtcggct gtcgcctgca tctctcgatc acgcagtcac
     4141 caatggcgcc tgaatcacgc ggcaatccgg gttcacggca gcagcagtgg cgccgtgaac
     4201 ccggaacggc tctattgata gcccttgggg aagggatcgg gcgcgatcag gtcggattcg
     4261 tagaccacat cggcctggcc atccgcacgc cactgcccga tgcgcagctt gctggagagg
     4321 tggtggttgg actccacctt cacatagccc tcgggcgccg tggtcatctc gtagccgggc
     4381 aaggccgccg ccgccttgtc gacatcgaaa ctgccggcac gctcgaccgc ggccttccac
     4441 agccatggac cgagataggc ggcctgggtg acatcgccga tcaccgagtt cggaccccac
     4501 atcttcttga attcggcaac gaactgctca ttgttggggt tcttcaggct ctgaaagtac
     4561 ttcatcgccg aatagaaacc cgcgaggttc tcaccgccga tgccaagcac ctcatcctcg
     4621 gtgaccgaga tggtgagcat ggtctgtttg ccggagttca ggcccgccgc gttcaactgc
     4681 ttgaaccagg cgacattgct gccaccgacc accgcggcat agatgacatc cggcttcttc
     4741 agcttgatct tgttgatcac cgagccgaac tgggtgtctc ccagcgcgat gtactcctcg
     4801 cccaccacct tgccacccag cttctgctcg atgtgcttgc gggcgatctt catcgaggtg
     4861 cgcggccaga tgtagtccga accgatcagg tagaagctct tggcaccctt ctccttcgcg
     4921 atccagtcga ggccggccag aatctgctgg gtggcctcct ggccggtgta gaagacattc
     4981 ttcgactgct ccagaccttc atagaaagtc ggatagaaca gcaggccatt ctccttctcg
     5041 aacaccggca gcgccgcctt gcgcgatgcc gaagtccagc agccgaacac ggccgccacc
     5101 ttgtcattga ccagcagctt cttcgacttt tcggcgaagg tcggccagtc actggcgcca
     5161 tcttcctgaa tcacctcgat cttgcggccg agcacgccgc ccatggcgtt gatctgcgcg
     5221 atcgccagct tctcggcctg gatcgaaccg gtctcactga tggccatggt gccggtcgcc
     5281 gaatgcagaa tgccgacctt gacgctgctg tcggtcaccg caaggccagt ggtgttgacc
     5341 tgcgcggtcg gcggagcgga cgccagcgcc gatccgatca gcaccccggc ggcgagcaga
     5401 ctcctggtca ctgtcgccgc accctcgacc catcgacctc gattgagttt tttcatcgac
     5461 ttcatcctcg tgtggagcat tggaaaagtt tcatcgttca cgtcactcaa gactcaatgt
     5521 ccgagcatcc agggtcgtgc cgtcttgcgg gtctggccct gcaatggcgt gccaccctgc
     5581 acggtggcgg ccgcagccgg tttgccccgc gtcgactggc agtggtgcac accactgcaa
     5641 ccgcagacgg atcgccgcag ctgcgccggc tcatggactg atctctcgtt gcgttcatgg
     5701 gcgacccgaa cggcgtggcc caccagtgcc agacgcggca gcgatggaat gcgcgcgctc
     5761 agggcgccgc agccgtcgca gtgggctggc tgttcgaact cgctcaacct gcgcagtgcg
     5821 gtaaagggtc cacactgttc gcagcggtat tcgtagatcg gcatggcctc accctcctca
     5881 ggacgcaacg gccatgtcga tgccgggcag caccctcttc tgcgggccat cggcattggg
     5941 cttgagatcg aaatcgaaga tctcggtcgg cagccacagc gtggcgcagg cgttggggat
     6001 gtccaccacc ccgctgatgt gcccctccac cggcgccgtg ccgagaatcg acagcgcctg
     6061 ggcaccgctg tagccgaact tcttcaggta ctcgatggca ttcaggcagg cctgccgata
     6121 ggcgaccgtg acgtcgaggt agtgctgggt gttgtgctcg tcgaccgaga tgccctcgaa
     6181 gatgaggtaa tcgcggtaag ccggggtcag cggactgggc tggaacagcg gattcttgat
     6241 cccgtacttt ttcaccccct ccttgatcag gctcaccttg atgtcgagat agcccgccat
     6301 ctcgatcgcg ccacagaagg tgatctcgcc atcgccctgg gagaagtgca gatcacccat
     6361 cgacaggcca ccatccttca cgtagaccgg aaagtagacc cgcgatccct tggtcagatt
     6421 cttgatgtcg cagttgccgc catggtcacg cggaggcacg gtgcgcgctg cttcgccggc
     6481 agcactcttg gcggcatcgc cctgcatccg tcccatcacc gcggcctgcg catcgggcgg
     6541 cagggcgagc gccggcaccc ggttcggatc ggtggcgatc agcgcggcct cgcgtctgtt
     6601 ccagttctcc agcaacgccc gcgagggcag acagccgatc agacccggat gcatgatgcc
     6661 ggcgaacttc acattgggca catggcgcga ggtggtgtag atgccgtgga aatcccagat
     6721 cgacttgcgc gcctcgggaa agtggtcggt gagaaacccg ccgccgttct gcctggagaa
     6781 cagcgcattg aacccccact gcgaatggtc gaaggtcccc acatcgagga tgtcgaccac
     6841 catcagatct cccggctcgg ccccttcgac accaatgggt ccgctcaggt agtggacctg
     6901 cagcagatcg acatcgcgaa tgtcgttggc gctgtcgttg ttgccaatct gcccgccggt
     6961 ccagtccatg cactcgacac ggaactcatc acccggcttg accatggcga tcattggcag
     7021 gtcacagtgc cagcggttgt ggatctgacc atcctgctcc cagggctttt tatccaaatc
     7081 cagcttcaca agcgttttca tgcagcggcc tcctcgagtt ggcgatttgc tgtcgatgac
     7141 aaaatcgctg gtcagcgaga tttgtcatgg cgcgagacta gcaagccgcc agggccgttc
     7201 tgaatacgtc ggattgcgta gagcgaggcg gcgcggtgac gccaggatga gggcagtcgg
     7261 cgggaagatg cggagaaatc cgctcgaccg ctgtcacgcg gccgagcgga aggtcaggcc
     7321 ggggccgtga agcgacgcgc ctcgatgacg ttggagagct gattgatgcg cgccagcacc
     7381 ttgcccagct catcgaagct ggtcacctcg atggtcagcc gcatggtggc ggtgccgctc
     7441 tccttgtgcg acagcgtgtt gaccgcgatg acgttgatcg aatcgcgcgc cagcacggtg
     7501 gtgatgtcgc gcagcagccc ggaacggtca taggcttcga tcaggatgtc gaccggatag
     7561 atctgcgcag tgcgaccgct ccagtccacc gcgatgatgc gtcccggctc attgtcctcg
     7621 agctgcagga tgttgttgca gtcggcacgg tgaatgctga cgccccgtcc gcgggtgatg
     7681 aagccggtga tggcatcgcc cggcaccggc ttgcagcagc tggcaaactg ggtcagcagg
     7741 ttgccgacac cctgaatcgc cactgcatcc ttggccagcg tcgcctcgct gcgcgcggca
     7801 cgggccagtg tcgtgggcag cagctccatc tgcgcccgct gcgcagtgcg gttttcgatc
     7861 cgctgcgcag cgttgagcag ttgactcagc cgcaaccggc ccgaaccgag gtcggcatgg
     7921 acgtcgtcga tgctcttgag attgacctca tcggccagtt gctgcggctc gatgccagtg
     7981 atcgccagcc gctggaactc cttttcgagc aagttgcggc catcgaggat gttctgctcg
     8041 cggttctgct ggcggaacca ctgctgcacc ttggcgcgcg cgcgcgcggt gcggatgtac
     8101 tgcaggctgg agttgagcca gtcgcggctg ggccggctgt gcggcccgcg caggatctcc
     8161 acccggtcgc cggtctgcaa cggatggttc agcggcacga tgcgaccgtt gaccttggcg
     8221 ccccggcaac tgttccccac ctcggtatgg acgtggtagg caaaatcgac cggcgtcgcg
     8281 ttgtgcggca ggctgaccac atggtcgtcg ggggtgaaga cgtagatgcg ctcctgccgc
     8341 tcgtaactgc gcaggtgctc ggcgatgttg ccgagcccgc ccatctcctc gtgccattcg
     8401 agggcctgtt gcagccaggc gatcttctct tcgtagtcct tcgacttgcg tgccttgccc
     8461 ggatccttgt acagccagtg ggcgcagacg ccgtactccg cctcttcgtg catctcttcg
     8521 gtgcgaatct gcacctcgac cacccgtccc tgcggcccga tgaccgcggt gtgcagcgag
     8581 cggtagccat tctccttggg gttggcgatg tagtcgtcga actcgttggg aatgtgcttc
     8641 cacaggctgt gcaccacccc gagggtggtg tagcagtcgc gtactgtcgg caccagcacc
     8701 cgcaccgcac gcacatcgta gatctgcgaa aaatcgagac ccttcttctg catcttgcgc
     8761 cagatgctgt agatatgttt tgaccggcca tagacctgca ccgccacgcc ggcactctgc
     8821 atctcctgct tgatctgctg gatgacccgc tggatatagt cgtcacggtc gcgccgccgc
     8881 tcatcaagca gacgggcaat cgactggtag gcatcgggtt cgagataacg gaacgagaga
     8941 tcttcaagct cccattcgat atgtcccacc ccaagccggt gtgccatcgg cacatagaag
     9001 tcgaacacct cgcgggaaac ccgttgttgt cgaacagggt caagaccctt gaccgctcga
     9061 atggcgcaag tgcgttcagc cagcttcagc agcaacaccc gcacatcatc gatcaccgcc
     9121 accagcatgg cgcgaatgtt cttgacctga tcctgattct tgccgaatac gctgtcgttg
     9181 ttggcgctcc cggcactgat tgccgccatg ctctgcacac cggcaatcag cttggcaatc
     9241 ttgctgccaa attcctgttt gacctgctca agcggcagtt tgccccggcg cactgcccga
     9301 tagagggtgg cggcgatgat ggaatcctga tccagatgca gctcggcgac gatccgagcc
     9361 atctccagtc ccatctgcag gcagtcgtag ctgtggtcgc gatactcctg tgatgaagga
     9421 tcacgctgct cgatctgctg cacccagcgc gccgcctgca acaccggctc gcgtacaagc
     9481 tgcggcacaa actgctccag acttgcacac caggattgca ggtcgatctc gccttcagct
     9541 gtgatcgggt ggtcgtcgcg tactttaacc atgatgaata tgacttttcg tctgctgagg
     9601 gaatgctatc tggcagcctg acagcgcgcc ggtttcagcc acgcacaaac aatgccatcg
     9661 actcgacatg ccgggtgtga ggaaacatgt c
//
LOCUS       DAORCQ010000086         9650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4879_length_9650_cov_5.443981, whole genome shotgun sequence.
ACCESSION   DAORCQ010000086 DAORCQ010000000
VERSION     DAORCQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_9650_cov_5.443981"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKI23_09020"
     CDS             <1..803
                     /locus_tag="PKI23_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNN37117.1"
                     /translation="AITPASRALLVGNSNGHPADWDAFTRLAQQHRLLLIEDSSEAIG
                     SRRGGRLVGSFGACALFDFSQPGALVCGEGGMVVTDDSALAARLRTLRSRHPADRHSV
                     VSAGLPCAQLAMSDLTAALGLAQLSRLPAILERRRQVEAFYREQILSFEGIKPPYQAP
                     DVEEVHWFLYLVHLGTRFSRSSRDAIVDDLHTEAIEAYPYCQPLHRQRAYLPAGTAVS
                     RLPVTEKLADRALALPFHAHLDEEQVAFIVATLKDASINVGAGTTIYL"
     gene            829..1134
                     /locus_tag="PKI23_09025"
     CDS             829..1134
                     /locus_tag="PKI23_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNN37118.1"
                     /translation="MPIITVRPSGNSFEASPGTSLLSALLAAGEAIEHKCEGDAKCGS
                     CHLFVHEGRKSLSKIARIENERLDTLIGIGSKSRLACQALLGEEDVTVELLGFASGF"
     gene            1192..1413
                     /locus_tag="PKI23_09030"
     CDS             1192..1413
                     /locus_tag="PKI23_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37119.1"
                     /translation="MNSSDQMIHVRFAPDGSVIQIGACPAGLTLQQWFNLLSNQAGFH
                     YQSLSGGRGLFRLPPELLERLSTKPVEVA"
     gene            1413..2273
                     /locus_tag="PKI23_09035"
     CDS             1413..2273
                     /locus_tag="PKI23_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNN37120.1"
                     /translation="MDPVLAKITTCAAAGQLVPYLGAGLIDLVEAAAVPPASSATLVK
                     KLTERVSVPPRSRNNLTGAAQFIENFKHRKTLRGLMSDAFAPRPAPPPLCHWLASLPR
                     LPLLVSAWYDDLMAQALAATGRNWGAVQGVSQAEQFGEWVNYFQADGSPASAGQAHDW
                     STLLYQPLGSIAPAANFLVSDSDFVEVLTEIDIQTPIPDEVKLRRDGRQFLFLGCRFD
                     DQLSRTFARQILKRSSHQHWAVLPGRLTRNEARFLAEVDIERIDLPLADFAALLTGSS
                     AESARLSAVT"
     gene            complement(2344..2925)
                     /locus_tag="PKI23_09040"
     CDS             complement(2344..2925)
                     /locus_tag="PKI23_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNN37121.1"
                     /translation="MSAFSSLLDEVRACTRCAAHLPLGPRPILQLHPKARVLIAGQAP
                     GRKAHATGLPFDDASGNRLREWLGVTREVFYDPEQIAILPMGFCFPGPGRGGDLPPRP
                     ECAPAWRKQLLAPLRHIEFTVVLGQYAQAYHFGDGRLPVTELVRSWHTHWPHRVPLPH
                     PSPRNTLWLRRNPWFECEVLPALRKRVAAVLAK"
     gene            complement(3418..4830)
                     /locus_tag="PKI23_09045"
     CDS             complement(3418..4830)
                     /locus_tag="PKI23_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HNN37122.1"
                     /translation="MSKPSQRKTAARNEAPYLSADERRAKGKALRDVVPRTSQAGWKP
                     PKNRRDPVEIIRESSAGRLENLIPIRYGRMSASPFAFYRGSAALMAADLSTTPTSGLR
                     VQACGDAHLMNFGGFATPERNIIFDINDLDETLPAPFEWDLKRLTASVVIAAQHLRLP
                     DSDAARVAIDVACEYRERMADYASMRALDVWYDRIDAERFIERATDPDLQKELRKRVT
                     ENIEEAQRKTEPDFLYPKLVSQEGTSPAIKDEPPLIFHPTPEQAPGLAGGAVEAVAVY
                     SKSLPEHVQTLFGRYHFFDYAVKVVGVGSVGTLCGICLFMAADDDPLFLQVKEARASV
                     LEPYAGKSLHANHGERVVVGQRIMQSASDVFLGWTHLLTGQDAYIRQLRDVKVSAVIE
                     DWDTEALRHYGRMCAHALARAHARSGDAAMLAGYMGAGQAFDDAIGEFAVEYADQNMS
                     DYRAFIKAIRDGRIRAQQDE"
     gene            complement(5214..5783)
                     /locus_tag="PKI23_09050"
     CDS             complement(5214..5783)
                     /locus_tag="PKI23_09050"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNN37123.1"
                     /translation="MSYRLTTITTKGGDGGETSLADGQRLPKDDARIEAIGTVDELNA
                     CIGLLRTEIEPGDIINRLLEEVQQQLFDLGGELALPGSQRLKGEYVAMLEEAQSVLND
                     ALPPLRNFVLPGGNRSAATAHLARTVCRRAERRLIHLDRQSPLNPASRIYLNRLSDLL
                     FVIARTLLRQQSEHEAQWGVDRESDFPRR"
     gene            complement(5845..>8255)
                     /gene="topA"
                     /locus_tag="PKI23_09055"
     CDS             complement(5845..>8255)
                     /gene="topA"
                     /locus_tag="PKI23_09055"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNN37124.1"
                     /translation="ARYEILPGKEKVVDELRHLAAEADTIYLATDLDREGEAIAWHLM
                     QAIGGDPARYRRVVFNEITQRAIRDAFDSPGTLDLNRVNAQQARRFLDRVVGYMLSPL
                     LWSKITRGLSAGRVQSVAVKLVVEREREIRAFLPEEYWTLDAELLGKGHTPVWFQVAK
                     EGDKKFQPGDEATTRKAMAALQGAEFILRQREDKPTQSRPGAPFITSTLQQAASTRLG
                     FGVKKTMTLAQRLYEAGYITYMRTDSTNLSAEAVASCRELIKSEFGATYLPPQPQGYA
                     SRSGAQEAHEAIRPTDVRAKPGKIAGLEPDQQRLYELIWRQFVACQMAPARFTSTTLV
                     VEAGDHELRARGRVMLFDGFLKVLPALAKGEDDRELPDLKEGARLDLQQLKPAQHFTK
                     PPARYSEATLVRELEKRGIGRPSTYAAIISTIQERGYVRLDQRRFHAEKMGELVTDRL
                     AECFTQLMDYGFTAQLEQQLDEVAAGQVEWKAVLDTFYDDFSSKLRLAERPQGGMRGN
                     QPTATDIPCPRCARPMLIRSGKTGIFLGCSGFALPAAEQCKETLNLLPGEEAILADAD
                     DEAESRQLRARHRCPLCGTAMESYLVDASQKLHVCGNNPDCAGYEIERGSYKLKGYDG
                     PSIACDKCGESMQLKSGRFGKFFACTAADCSNTRKLLKSGQAAPPKSAPIPMPELRCA
                     KVNDHYVLRDGAAGLFLAASQFPKHRETRAPRVRELLPHADQLDPKYAHLLSAPLTDD
                     QGNDTFIRFSRKEGALYLSSEADDKATGWQARYQNGKWVASSGQKRDSAKPATATRKK
                     RKVA"
     assembly_gap    8256..8355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8356..8654)
                     /locus_tag="PKI23_09060"
     CDS             complement(<8356..8654)
                     /locus_tag="PKI23_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016971417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNN37125.1"
                     /translation="MGRSLVIVESPAKAKTINKYLGREFIVKSSVGHIRDLPVSGGEA
                     SKERGAKRGKTKAAADDKTPVVSPLVRRMGVDPEQGWQARYEILPGKEKVVDELR"
     gene            8881..>9650
                     /locus_tag="PKI23_09065"
     CDS             8881..>9650
                     /locus_tag="PKI23_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010953311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN37126.1"
                     /translation="MSYRDLHRQSITHPEQFWGDRAHEISWYRAPTTLLGQDEHGIDR
                     WFVDGELNSCYLAVDFHIEQGRGEQTALIYDSPVTGSQARYSFHQLRDRVAKVAGFLQ
                     DLGVAQGDRVVIYMPMVPEAVIAMLASARLGAIHSVVFGGFAPRELALRIDDAKPRVL
                     LSASCGIEVDRVIPYKPLLDEALRLSQHRPQHCVILQRPQHPATLLPGRDHDWHSLES
                     TAEAAEPVPVRATDPLYILYTSGTTGRPKGVVRDNGGHA"
BASE COUNT         1602 a         3164 c         3029 g         1755 t
ORIGIN      
        1 ccgccatcac acccgccagt cgcgcgctgc tggtcggcaa cagcaatggc catcccgccg
       61 attgggacgc cttcacccgg ctggcgcaac agcatcgact gctgctgatc gaggactcca
      121 gcgaagccat cggctcacgc cgcggtgggc ggctggtcgg cagtttcgga gcctgcgcgc
      181 tgttcgactt ctctcagccc ggtgcgctgg tctgcggcga aggcggcatg gtggtgaccg
      241 atgacagcgc actcgcggcc cgtctgcgca ccctgcgcag tcgccatccc gccgaccgtc
      301 actcggtggt cagcgctggc ctgccctgcg cacagctggc catgagcgac ctcacggccg
      361 cgcttggtct ggcgcaactg tcgcgactgc cggccattct cgagcgtcgg cggcaggtcg
      421 aggcgttcta ccgtgagcag atcctctctt tcgaagggat caaaccaccc tatcaggcac
      481 ccgatgtcga agaggtgcac tggtttctct atctggtcca cctcggcacc cgcttcagcc
      541 gttcgagccg cgacgccatc gtcgatgacc tgcacaccga ggcgatcgaa gcctatcctt
      601 actgccagcc actgcaccgc cagcgcgcct atctgccggc cggcaccgcc gtctcgcggc
      661 tgccggtcac cgaaaaactg gccgaccgcg ccctggcgct gccattccac gcccatctgg
      721 acgaagagca ggtggcgttc atcgtcgcca ccctcaagga tgcctcgatc aacgtgggcg
      781 ccggcaccac catctatcta tgaccccgcg atcaacagga gatcgaccat gcccatcatc
      841 accgtcaggc catcgggaaa cagcttcgaa gccagccccg gcaccagcct gctgagtgca
      901 ctgctcgccg ccggcgaggc catcgaacac aagtgcgagg gcgacgccaa atgtggctcg
      961 tgccacctgt tcgtgcacga aggccgcaag agcctctcca aaattgcccg catcgagaac
     1021 gaacggctcg acaccctgat cggtatcggc tccaaatcgc gcctggcctg ccaagccctg
     1081 ctcggagagg aggatgtcac ggtcgaactg ctcggcttcg cctccggctt ctgaagcgac
     1141 ctcgttgact cctcaggcaa cccatcccca cccagaccgg agatcacccc gatgaacagc
     1201 agcgaccaga tgatccatgt ccgtttcgcg ccggatggct cggtgattca gatcggcgcc
     1261 tgtccggccg gcctcacgct gcaacagtgg ttcaacctgc tgagcaacca ggccggattc
     1321 cactaccagt cactgtcagg tggacggggg ctgttccggc tgccaccgga gttgctggag
     1381 cggttgtcga caaaaccggt cgaggtggcc tgatggaccc ggtcttggcg aaaatcacca
     1441 cctgtgcggc cgccggccag ttggtgccct acctgggtgc cggcctcatt gacctggtcg
     1501 aagccgcggc cgtaccgccc gcctcatcgg ccacgctggt caaaaaactg accgaacggg
     1561 tcagcgtgcc gcccagaagc cgcaacaacc tgactggcgc cgcgcagttc atcgagaact
     1621 tcaagcaccg caagaccctg cgcggcttga tgagcgacgc cttcgcccca cggcccgccc
     1681 cgccgccgct ctgccactgg ctggccagtc tgccccggct gccgctgctg gtcagtgcct
     1741 ggtatgacga cctgatggcg caggcattgg cggccaccgg gcgcaactgg ggcgcggtgc
     1801 agggggtcag ccaggccgaa cagttcggcg aatgggtcaa ctactttcag gccgatggca
     1861 gcccggccag cgccgggcag gcccacgact ggtccaccct gctctaccag ccgctcggca
     1921 gcattgcgcc cgccgccaac tttctggtct ccgactcaga cttcgtcgag gtgctgaccg
     1981 aaatcgacat ccagacgccg attcccgatg aagtgaagct gcgccgcgac ggccgccagt
     2041 tcctctttct cggctgccgc ttcgacgatc aactgagccg caccttcgcc cggcagattc
     2101 tcaaacgctc ctcgcatcag cactgggccg tgctgccagg cagactcacc cgcaacgagg
     2161 cgcgctttct cgccgaggtc gacatcgaac gcatcgacct gcccctggcc gactttgccg
     2221 cgctgttgac cggcagcagc gcagagagtg caaggttgag cgccgtgacc tgaatggcgc
     2281 agactccctc actgcgaaca gcgcctgtgc gattttggcc ggtgatccgc ctgtcgggcc
     2341 gtctcatttt gcgagcaccg ctgccacgcg cttgcgaagc gcggggagaa cttcacactc
     2401 gaaccagggg ttccggcgca accagagggt gtttcgcgga ctgggatgcg gcagcggaac
     2461 ccggtggggc caatgggtgt gccatgacct gaccagttcg gtgacaggca agcgcccgtc
     2521 accgaagtga tacgcctgcg cgtactgccc gagcaccacg gtgaactcaa tgtgacgaag
     2581 cggcgcaaga agctgtttgc gccaggcagg cgcacactcg gggcgtggcg gaaggtctcc
     2641 cccccttccc ggaccgggga agcagaagcc catcggcaga atcgcgatct gctccggatc
     2701 gtaaaacact tcgcgtgtca cccccagcca ctcgcgcaag cggtttccac tggcgtcatc
     2761 gaacggcagg cccgtggcat gagcctttct gcccggcgcc tgcccggcaa tcagaactct
     2821 ggccttcgga tgcaactgaa gtatcggccg aggccccagc ggcaagtgcg ccgcgcagcg
     2881 ggtgcatgct cgaacctcat caagcaggga cgaaaaggcg ctcatggtca cgacctcccg
     2941 caacaagcca acgaagctgt aaaagcatca ttccgccgcg cctgcctgct ccggtggccg
     3001 gcgaacggtg cggcttgttt ggcatcgacc cgacagcaaa aacagggtcg gactgagcat
     3061 cccgggattg atgaacactt tttaaggtcg acactgcctg tgaaggtgtc agtggcaggg
     3121 tgggcaaagc gcagcgtgcc cacacagaaa aacgcgcgtc atcgcgcgaa tcgggttgcg
     3181 aatcgttgaa cccgcgtggg cacgacctga aggcctttgc ccaccctgaa aatcgctgaa
     3241 gcgaggcaaa cccgatgtcg cgccagcgga aaaccggaga cactgttttt cggcatctgg
     3301 cttgagaacc tggccaagaa gcgtggtctt tccccggttt tcgctcggca atgctggaag
     3361 aaaacgggga gattggcgtc cgtcatggca tcacccctgc tgcggggcat cacagcgcta
     3421 ctcgtcttgt tgagcacgaa ttcgcccgtc gcggatcgcc ttgatgaagg cgcggtagtc
     3481 gctcatgttc tgatcggcat actctactgc gaactcgccg atcgcgtcat cgaaggcctg
     3541 cccggcgccc atgtagccgg cgagcatcgc cgcatcgccg gagcgggcgt gtgcgcgcgc
     3601 cagcgcatgt gcgcacatgc gcccgtagtg gcgcaacgct tccgtatccc agtcctcgat
     3661 gaccgcagac actttcacgt cgcggagctg ccggatatag gcgtcctgtc cggtcagcag
     3721 gtgcgtccag ccgagaaaca catcgctcgc cgactgcatg atgcgttggc caaccaccac
     3781 gcgctcgcca tgattggcgt gcagactctt gccggcatag ggctccagca ccgaggcgcg
     3841 cgcctctttc acctgcagga acagcggatc gtcgtccgcc gccatgaaca gacagatgcc
     3901 acacaaggtg ccgacgctgc ccacgccaac gaccttgacg gcgtagtcga aaaagtgata
     3961 ccggccgaac agggtctgaa catgctccgg cagactcttg ctgtagacgg cgaccgcctc
     4021 caccgcccca cccgccagcc ccggcgcctg ctccggcgtg ggatgaaaga tcagcggcgg
     4081 ctcgtccttg atcgcggggc ttgtgccttc ctgcgacacc agcttcgggt acaggaaatc
     4141 gggctccgtc ttgcgctggg cttcctcgat gttttcggtg acgcgtttgc gaagctcctt
     4201 ctgcagatcc ggatcggttg cccgctcgat gaagcgttcc gcgtcgatcc ggtcatacca
     4261 gacgtcgagc gcgcgcatcg acgcgtaatc ggccatccgc tcgcggtatt cgcaggccac
     4321 atcgatggca acgcgcgccg catcgctgtc gggcagacgc agatgctgcg cggcgatcac
     4381 cacgctcgcg gtcaggcgtt tcaaatccca ttcgaacggc gccggcaggg tttcgtccag
     4441 gtcattgatg tcgaagatga tgttgcgctc gggcgtcgcg aaaccgccga agttcatcaa
     4501 atgcgcgtcg ccgcacgcct gcacccggag cccactggtc ggcgtcgtgg agagatccgc
     4561 ggccatcagc gccgccgagc ctcggtagaa ggcaaatggc gaagcggaca tgcggccgta
     4621 gcggattgga atgaggttct ccagccgacc ggcactggat tcacggatga tctcgaccgg
     4681 atcccggcga ttctttggcg gcttccatcc cgcctgcgag gtcctcggca ccacatcgcg
     4741 cagcgccttg cccttggcgc gacgctcgtc agcggacaga tagggcgcct cgtttcgagc
     4801 cgcggttttg cgctgactgg gcttggacat cttcactcct gtctgtctag cagtttgttg
     4861 aaatacttat gaattttaat tttctgaact gggcgtgcgc tgaaaaatcg gggttccgga
     4921 taccgaaaac gccctcaccg cagtgccatc ggccctttca caggccccag tggggtgctg
     4981 ctccggattt ttcgcattca tccggccact cctgtcagca gattgtgaat tcgcaaccga
     5041 ttgattcaaa aagattcgct ctcacgaaag ccctccctgc cacagacggc accccgaggc
     5101 accgctcttt gaaatggccc gctcgagcgc gcggacgcgg ttggccagac tttacgacat
     5161 cgcacccgaa aacagaatgt gcgatgaccg aagtcggcgg attctgccgc gcttcagcgc
     5221 cttgggaaat cgctttcgcg gtcgacgccc cactgggctt cgtgttcgct ctgctgacgc
     5281 agcagggtgc gggcgatgac gaacagcagg tcggagagcc ggttgaggta gatgcggctg
     5341 gcagggttga ggggcgattg acggtcgaga tggatcagcc gccgttcggc gcggcggcag
     5401 acggtgcggg ccagatgggc ggtcgcggcg ctgcggttgc caccgggcag cacgaagttg
     5461 cgcagcggcg gcagggcgtc gttcagcacg ctctgggcct cttcgagcat ggcgacatat
     5521 tcgcccttga gacgctggct gcccggcagg gccagttcgc cgcccaggtc gaacagttgc
     5581 tgctgcacct cttcgagcag ccggttgatg atgtcgccgg gttcgatctc ggtgcgcagc
     5641 aggccgatgc aggcgttgag ttcatcgacg gtgccgatgg cttcgatgcg ggcatcgtcc
     5701 ttgggcagac gctggccgtc ggccagactg gtctcgccac catcgccgcc cttggtggtg
     5761 atggtggtca ggcggtagct catgcactgt ctcgctgctg ggggttgagg gggatcatcc
     5821 gctcgccact ttcggaacca cgcatcaggc caccttgcgt tttttgcggg tggcggtggc
     5881 aggtttggcg ctgtcgcgtt tctggccgct ggacgcaacc cacttgccat tctgataacg
     5941 cgcctgccag ccggtggcct tgtcgtcggc ctcgctgctg aggtagaggg cgccctcctt
     6001 gcggctgaag cggatgaagg tgtcgttgcc ctggtcgtcg gtcagcggcg cgctgagcag
     6061 gtgggcatat ttggggtcga gctgatcggc atggggcagc agttccctga cccgcggtgc
     6121 ccgggtctcg cggtgtttgg gaaactggct ggcggccaga aacaggccgg cggcaccgtc
     6181 gcgcagcacg taatggtcgt tgaccttggc gcagcgcagt tcgggcatcg gaatcggggc
     6241 gctcttgggc ggagccgcct gaccactctt cagcagcttg cgggtgttgc tgcaatccgc
     6301 cgcggtgcag gcgaagaatt tgccgaagcg gccgctcttc agttgcatcg attcgccgca
     6361 tttgtcgcag gcgatcgagg ggccgtcata gcccttgagc ttgtagctgc cccgttcgat
     6421 ctcgtagccg gcgcagtcgg ggttgttgcc gcagacgtgc agtttctggc tggcgtcgac
     6481 caggtagctc tccatcgcgg tgccgcagag cgggcagcgg tggcgggcgc gcaactggcg
     6541 cgattcggct tcgtcgtctg catcggccag aatcgcctct tcgcccggca gcaagttgag
     6601 ggtctccttg cactgttcgg ccgccggcag cgcaaagcct gagcaaccaa gaaagatgcc
     6661 ggtcttgccg gagcggatca gcatcggccg tgcgcaacgc ggacagggga tgtcggtggc
     6721 ggtcggctgg ttgccgcgca tccccccttg cggccgctcg gccagccgca gcttgctgct
     6781 gaagtcgtcg tagaaggtgt cgagcacggc cttccactcc acctgaccgg cggccacttc
     6841 gtcgagctgc tgttcgagct gcgcggtgaa gccgtagtcc atcaactggg tgaagcattc
     6901 ggccagccgg tcggtcacca gttcgcccat cttttcggca tggaagcggc gctggtcgag
     6961 ccgcacgtag ccacgctcct ggatggtcga gatgatggcg gcataggtcg aggggcggcc
     7021 gatgccgcgc ttctccagct cccgcaccag ggtcgcctcg ctgtagcggg ccggcggctt
     7081 ggtgaagtgc tgcgccggtt tgagctgttg caggtcgagt ctggcgccct ccttcaggtc
     7141 gggcagttca cggtcatcct cgcccttggc cagtgccggc agcaccttga gaaagccatc
     7201 gaacagcatg acccggccgc gcgcgcgcag ttcgtggtcg cctgcctcga ccaccagcgt
     7261 ggtggaggtg aagcgcgccg gcgccatctg gcaggcgacg aactgccgcc agatcagttc
     7321 gtaaagccgc tgctgatccg gctccagtcc ggcgatcttg ccgggtttcg cccgcacgtc
     7381 agtcggacgg atcgcctcgt gcgcctcctg cgcgccgctg cggctggcat agccctgtgg
     7441 ctgcggcggc aggtaggtgg cgccgaattc gcttttgatc agttcgcgac agctcgccac
     7501 cgcctcggcg ctgaggttgg tcgagtcggt gcgcatgtag gtgatgtagc cggcctcgta
     7561 gagccgctgc gccagggtca tggtcttctt cacgccgaag ccgagacggg tgctggccgc
     7621 ctgttgcagc gtggaggtga tgaagggcgc accgggtcgg gactgggtcg gcttgtcttc
     7681 gcgctggcgc aggatgaact cggcgccttg cagggcagcc atcgccttgc gggtggtggc
     7741 ttcatcaccc ggctgaaatt ttttatcgcc ctctttggcg acctggaacc agaccggcgt
     7801 gtggcccttg ccgagcagtt cggcatcgag cgtccagtac tcctcgggca gaaaggcgcg
     7861 aatctcccgt tcgcgctcga ccaccagctt gacggccacc gactgcaccc gcccggccga
     7921 caggccacgg gtgatcttgc tccacagcag cggcgagagc atgtagccca ccacccggtc
     7981 gagaaaacgg cgcgcctgct gggcattgac ccggttgagg tcgagcgtgc cggggctatc
     8041 gaaggcgtcg cggatggcgc gctgggtgat ctcgttgaac accacccgcc gatagcgggc
     8101 cggatcgccg ccgatcgcct gcatcaggtg ccaggcgatc gcctcaccct cgcggtcgag
     8161 gtcggtggcg aggtagatgg tgtcggcctc ggcggccagg tggcgcagct catcgaccac
     8221 cttctccttg cccggcagaa tctcgtagcg cgcctnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnntggcg cagctcatcg accaccttct ccttgcccgg cagaatctcg
     8401 tagcgcgcct gccagccctg ctccggatcg acccccatgc ggcgcaccag cgggctgacc
     8461 acgggcgttt tgtcgtcggc cgctgccttg gtcttgccac gcttcgcgcc gcgctccttg
     8521 ctcgcctcgc cgccgctgac cggcaggtca cggatgtggc cgacgctcga tttgacgatg
     8581 aactcccgtc ccagatattt gttgatcgtc ttggccttgg ccggtgattc gacgatcacc
     8641 agggatctgc ccataccgtt gtaaccttgt gttcgattga aaaaggatga atagggtccg
     8701 ctggcccggg tgcagtcaca ggccagcgcg ctttttatta tagaaagtat cgaaccaccg
     8761 gctgtcaagg catcttcacc gctgcggcgg ttgccaaggc ggtgcctgcc ctttaagtta
     8821 gccggcaaat ccgcagaccg cactcagcac cgcgcttcac gccaaggcag gttcccttcg
     8881 atgagctacc gcgaccttca ccgacagtcg atcacccatc ccgagcagtt ctggggtgac
     8941 cgtgcccatg agatcagctg gtaccgcgcc cccaccacac tgctgggcca agatgagcat
     9001 ggcatcgacc gctggtttgt cgatggcgag ctcaacagct gttacctggc ggtcgacttt
     9061 cacatcgagc agggccgcgg cgagcagacg gcgctgatct acgactcgcc ggtcaccggt
     9121 agccaggcgc gttacagctt ccatcagctg cgcgaccgcg tcgcgaaggt cgccggattt
     9181 ctgcaggatc tgggcgtcgc tcagggcgac cgggtggtga tctacatgcc gatggtgccc
     9241 gaagcggtga tcgccatgct ggccagcgcc cggctcggcg ccatccattc ggtggtgttc
     9301 ggcggttttg cgccacgcga gctggcattg cgcatcgacg atgccaagcc cagggtgctg
     9361 ctcagcgcct cctgcggcat cgaggtcgac cgggtcattc cctacaaacc gctgctcgac
     9421 gaggcgctgc gcctgagcca gcaccgcccg cagcactgtg tcattctgca gcggccccag
     9481 cacccggcca cgctgctgcc gggccgcgac cacgactggc acagcctcga aagcacggcc
     9541 gaagccgccg agccggtgcc ggttcgtgcc accgatccgc tctacattct ctacacctcc
     9601 ggcaccaccg gccgccccaa gggggtggtg cgcgacaacg gcggccatgc
//
LOCUS       DAORCQ010000087         9596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_4919_length_9596_cov_5.672990, whole genome shotgun sequence.
ACCESSION   DAORCQ010000087 DAORCQ010000000
VERSION     DAORCQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4919_length_9596_cov_5.672990"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PKI23_09070"
     CDS             <1..605
                     /locus_tag="PKI23_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNN37127.1"
                     /translation="IEIDLNQIREPLLACPNDPDDVRPLSSVAGARIDEVFIGSCMTN
                     IGHFRAAGKMLEKASAIPTRLWISPPTKMDQQQLMEEGFYNTFAKVGARTEMPGCSLC
                     MGNQARVAAKSSVVSTSTRNFPNRLGDGANVYLASAELAAVAAILGALPTVEQYMQYA
                     NTLNSMAPEIYRYLNFDQLDNFVKAANQVKIPAVNVSVAG"
     gene            complement(683..1468)
                     /gene="fnr"
                     /locus_tag="PKI23_09075"
     CDS             complement(683..1468)
                     /gene="fnr"
                     /locus_tag="PKI23_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNN37128.1"
                     /translation="MSESRNLAAISCHHQPGKISRVACHECGLAPLCLPLSLTDEGVS
                     KLDSIIQRSRPLQKGDYLFHQGDIFDSVFAIRTGSIKSYTITDSGQEQITGFYLPTEL
                     AGLSGLNCFTYPVSVVALETTSVCAIPYIRLEKLFDELPALRQQVMHSMSKELRDYQD
                     MMLVMGQKSAEARIATLLVNLSMRFKRRGFSAHHFRLSMSRNEMGNYLGMAVETVSRV
                     FTRFQKQGLLRVEGKDVEILQPAALSTMAGSSASTMQEQRASH"
     gene            complement(1582..2256)
                     /locus_tag="PKI23_09080"
     CDS             complement(1582..2256)
                     /locus_tag="PKI23_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNN37129.1"
                     /translation="MFESLGYGAAFMFGLLGSSHCLGMCGGIMGALGMFSSSSVRPIE
                     RMLLILGYNLGRIFSYAVAGLLVGLLSSWLQARFFLFATLVQVMAALLLIAMGGYLAG
                     FQRLLLPIERIGQQLWRRVAPVANQMLPVRRFRTALLAGLIWGWLPCGLVYATLGWAA
                     TAESAWQSALTMLAFGLGTLPLMFVSGLAFFRFRLLLAHPYLRSLNGLLVALFGLWNL
                     SRLLAG"
     gene            complement(2249..2455)
                     /gene="ccoS"
                     /locus_tag="PKI23_09085"
     CDS             complement(2249..2455)
                     /gene="ccoS"
                     /locus_tag="PKI23_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNN37130.1"
                     /translation="MEILFILIPLSLLLVAVMLWAFAWSVRTGQFDDLQRHGHDLMFD
                     DEPPGQQATEEGDPIQRERPPADV"
     gene            complement(2460..4961)
                     /locus_tag="PKI23_09090"
     CDS             complement(2460..4961)
                     /locus_tag="PKI23_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNN37131.1"
                     /translation="MKCYHCDLPIAQPDPLQVEIEGTLRPMCCPGCAAVARSIVEGGL
                     DRFYRLRDRPALNPDDPALSGLPDFSEYDHPAVAAELVQVAEDGSHHVSLLLAGMNCA
                     ACVWLIESGLKAQSGIRSISVNFDQRRAEVNWQADQIRLGEIVATLHRLGYRAEPFTV
                     TAKEQLLQRERRDLLIGLGFAGIGMMQVMMYSGALYLDDFLEFERNYQTLLRWASLLL
                     TLPVVAISSRPFFSGAWRDLRQRRPGMDVPVALAIALAFLVSVWATFTQGPAVYFDSV
                     VMFVFFLLLGRFLERTVRERAGLAALDLRSRLPRLATRVCEKGEESVPLRRLQVGDLL
                     LIRPGEVIPTDGVVQNGRSSVDESLLTGERLPVLREVGDRLLGGSINLEQPLWISVSC
                     GVEHSRLAMIERLLQQAQADKPHIIQLTDRIASHFVTVMLGCAASVYLFWWWQGSADA
                     FWITLSVLVITCPCALSLATPTVLTAATLALQKQGFLAIKGHVIESLNQIDQVVFDKT
                     GTLTEQRLRLLRTLPAAGLDADGCLALAAALEGASRHPIAATLIDAAASRALPVSRVI
                     ELCSSAGQGVEGVWRGESVRIGTPEFIRALLRSEQPVTPPSADGKWLALASESQLLAW
                     FELEEPQRRDAAASLRQLASRGLHLALMSGDVPAAVAPLAASLNLVDWQASMTPEAKL
                     AAVRARQQSGAKVLMVGDGVNDAPVLAGANLAVAIDEGSDLAKSSADAIILNGRLSSL
                     VAAFVVADRSSRVIRQNLLWAFGYNLLAIPFAAAGWVSPYLAALGMSLSSLLVVANAL
                     RLLRPVATGLTEAPLTLSSALPGPPRAVPLSQTVE"
     gene            complement(4958..5482)
                     /locus_tag="PKI23_09095"
     CDS             complement(4958..5482)
                     /locus_tag="PKI23_09095"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNN37132.1"
                     /translation="MSSATSTLPAPWYRQFWPWFLIALPLLSVVAGLILLGFALDSRD
                     SLVRDDYYKEGMMINQSIRRDQAAAAARLSARIELSADARELLVVLEGELTDWPSALT
                     LELDHPTLAEQDQRFTLVQVAPRHYRTLLERPPHGRFHLSVAPATLDRGDWRLKGEAE
                     LPRPVELRAAFKPG"
     gene            complement(5498..6922)
                     /gene="ccoG"
                     /locus_tag="PKI23_09100"
     CDS             complement(5498..6922)
                     /gene="ccoG"
                     /locus_tag="PKI23_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNN37133.1"
                     /translation="MPQRLPVREIDPKQPPVTDSRELDLYEKREKIYTRKIEGFFQRI
                     RLYTGWPLLLGYFLLPWINWNGHQSLLWSLPERKFYILNWTFWPQDFVFLAWLLIICA
                     FGLFFVTNWLGRVWCGYTCPQTVWTAIYMWMEQFAEGSRNQRIKLDQAPWSAEKLFKK
                     GLKHAMWLGFGFWTGLTFVGYFTPIRELLPDLVLLQAHPVAAFWTLFFMLATYVNAGW
                     MREQVCKYMCPYARFQSAMFDRDTLIISYDAARGEPRGSRKRGDAAEKQELGDCINCT
                     VCVQVCPTGIDIRDGLQYECIGCALCIDGCNEVMDKMGYPRGLIRYTTLAELETGKRH
                     ILRPRLVGYGVAILVMIGLFGYRLYQRIPLELDIIRDRNQLFTMSSDGGIENSYTLKI
                     LNMDSAPHRYRITATGIDGLKIQGATEAEVNGSEVLSLPLRLTVPAQQLGQGSRKILF
                     SLEAIDRDDYRITQESRFIGPAQR"
     gene            complement(7050..7934)
                     /gene="ccoP"
                     /locus_tag="PKI23_09105"
     CDS             complement(7050..7934)
                     /gene="ccoP"
                     /locus_tag="PKI23_09105"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNN37134.1"
                     /translation="MSTFWSLWVMVLVAVTAVGSWWLLQSNRKVPPGEGATTGHEWDG
                     IEELNNPLPYWWYMMFILSLLFLVAYLVLYPGFGNFPGVLNWSSTGAWQKEVQEAEKE
                     FGPLFVRYGAMSVEQLQQEPQALKMGQRIFATNCSVCHGSSATGSFGFPNLTDSEWLY
                     GGTAEAIETTILSGRRGQMPPWGPILGDEKVKQVTAHVVNLSGGTADPKLAEAGKALF
                     ASTCTACHGMDGKGNQLIGAPDLTNAIWLYGGAPGQIEHSIAAGRAGQMPTFEQTLGA
                     QKVRIVAAYVYSLSQPKK"
     gene            complement(7931..8104)
                     /locus_tag="PKI23_09110"
     CDS             complement(7931..8104)
                     /locus_tag="PKI23_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNN37135.1"
                     /translation="MEINTVRAVSTVVVMLLFVAICLWAYSSKRKSAFDEAAQLPFDD
                     EAETTPNSRGKGQ"
     gene            complement(8108..8716)
                     /gene="ccoO"
                     /locus_tag="PKI23_09115"
     CDS             complement(8108..8716)
                     /gene="ccoO"
                     /locus_tag="PKI23_09115"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNN37136.1"
                     /translation="MKHDLIEKNLGVMIVLVAIVISFGGLAEIPPLFFIQGNTQPVAG
                     LTPLKPLELAGRDIYIREGCHVCHTQMVRPFRSETERYGHYTVAGEQVYEHPFLWGSK
                     RTGPDLARVGGRYSDDWHRAHLYNPRDVVPESNMPAFTWLFDSEVDASQIQAKMRALR
                     TVGVPYSDAEIEAAPQALQSRSEADAMVAYLQQLGTVMQSVK"
     gene            complement(8730..>9596)
                     /locus_tag="PKI23_09120"
     CDS             complement(8730..>9596)
                     /locus_tag="PKI23_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNN37137.1"
                     /translation="SYSVYSGTIDAMVQWWYGHNAVGFYLTAGFLGMMYYFIPKQAER
                     PVYSYRLSIVHFWALICVYMWAGPHHLHYSALPDWAQSVGMVMSLVLLAPSWGGMING
                     IMTLSGAWEKLRTDPIIKFLIVSLSFYGMSTFEGPMMSIKTVNALSHNTDWTIGHVHS
                     GALGWVAMISIGSLYHMIPIMFGKKQGEMFSIPLINFHFWLSTIGTVLYIASMWVNGI
                     MQGLMWRAVNTDGTLTYSFAEAVEASYPGYVVRFVGGAIFFFGMLVMAYNVWKTARPA
                     DASITHAIPQAG"
BASE COUNT         2069 a         3037 c         2830 g         1660 t
ORIGIN      
        1 tgatcgagat cgatctgaac cagatccgcg agccgctgct cgcctgcccc aacgacccgg
       61 atgatgtgcg gccgctgtcg agcgtggccg gcgccaggat cgacgaggtg ttcattggct
      121 cctgcatgac caacatcggc cacttccgcg cggccggcaa gatgctggag aaggcctcgg
      181 caatcccgac gcggctgtgg atctcgccac cgaccaagat ggatcagcag cagttgatgg
      241 aagaggggtt ctacaacacc ttcgccaagg tgggtgcgcg caccgagatg cccggctgtt
      301 cactctgcat gggcaaccag gcacgggtgg ccgccaagag cagcgtggtc tccacctcga
      361 cccgcaactt ccccaaccgt ctcggcgatg gcgccaatgt ctacctcgcc tcggccgaac
      421 tggcggcagt ggcggcgatc ctcggcgcac tgccgaccgt cgagcagtac atgcagtatg
      481 ccaacacgct gaacagcatg gcgcccgaga tctaccgcta tctgaacttc gatcagctcg
      541 acaacttcgt caaggcggcc aaccaggtga agattccggc cgtcaacgtc tcggtcgcgg
      601 gctgaccctc cggcccaaag cgaaaacccc gccagatggc ggggttttca tttctgtatc
      661 tgataaaact gcctgcgccg catcagtggc tggcacgctg ctcctgcatc gtgctggcac
      721 tgctgccagc catcgtagag agtgcggcgg gctgcagaat ctcgacatct ttgccctcga
      781 cccgcagcag tccctgcttt tgaaaacggg tgaagacccg gctgacggtc tcgacggcca
      841 tccccagata gttgcccatc tcattgcgtg acatgctgag ccggaaatgg tgtgccgaga
      901 agccacgccg tttgaaccgc atcgagaggt tcaccagcaa ggtggcgatg cgcgcttcgg
      961 cactcttctg gcccatgacc agcatcatgt cctggtagtc gcgcagctcc ttgctcatcg
     1021 agtgcatcac ctgttggcgc agggctggca gttcgtcaaa cagcttttcg agacggatgt
     1081 agggaatcgc gcagaccgag gtggtttcga gcgccaccac cgaaaccggg taggtgaagc
     1141 agttgagccc cgacagtccg gccagttcgg tgggcagata gaagccggtg atctgctctt
     1201 ggccactgtc agtgatggta tagctcttga tcgagccggt acgaatcgcg aagaccgaat
     1261 cgaagatgtc gccctggtga aacaggtagt cacccttttg cagtgggcgg ctgcgctgga
     1321 tgatcgagtc gagtttgctg acaccttcat cggtcaacga aagtggcagg cagagcggcg
     1381 ccagtccgca ttcatgacag gcaacccggc tgatcttgcc gggttgatgg tgacaggaga
     1441 tggcggcgag attgcgtgac tctgacatgg atcctgctca tggtggactg ctgggcgtct
     1501 cgtctgatca tgctgcgtgc ggatcagatc gacaacgtac ttgagaagta taacgaaaag
     1561 caccacctca agaaaaaact gtcagcctgc cagcaatcga ctcaggttcc acagcccaaa
     1621 gagcgccacc agcaatccgt tgaggctgcg caaataggga tgagccagga gcaaacgaaa
     1681 tctgaaaaaa gccagaccac tgacgaacat cagcggcaag gtgccaagac cgaatgcaag
     1741 catggtgagc gcgctctgcc aggcactctc ggcggtggct gcccagccta gggtggcata
     1801 gaccagacca cagggcagcc atccccatat gagccccgcc agcagagcag tccggaaccg
     1861 tcgcaccggc aacatctggt tggcaaccgg cgccaccctt cgccagagct gctggccgat
     1921 ccgctcgatc ggcagcagca accgctggaa accggccaga tagcccccca ttgcgatcaa
     1981 cagcagtgct gccatgacct gaaccaatgt ggcgaagaga aaaaaccgcg cctgcagcca
     2041 gctcgacagc agcccgacca gcaatccggc caccgcataa ctgaagatgc gaccaaggtt
     2101 gtagcccagg atcagtagca tgcgctcgat gggccgcacg gagctggagg agaacatgcc
     2161 gagcgccccc atgatgccac cgcacatgcc gagacagtgg ctgctgccga gcagcccgaa
     2221 catgaaggcc gcgccataac ccaggctctc aaacatcggc aggcggccgc tcgcgctgta
     2281 tcggatcgcc ctcttcggtc gcctgctggc cgggcggctc gtcgtcgaac atcaggtcat
     2341 ggccatgtcg ctgcagatca tcgaattggc ctgtgcgaac cgaccaggca aaggcccaca
     2401 gcatcacggc caccagcagc agcgacagcg gaatcagaat gaacagaatc tccatgccgc
     2461 tactccaccg tttgcgagag cggaacggcg cgcggagggc ccggcagcgc cgatgacagc
     2521 gtgagtggtg cttccgtcag cccggttgca accggtcgca gcagccgcag ggcattggcc
     2581 accaccagca gcgagctgag gctcatcccg agcgccgcca gatagggcga cacccagccg
     2641 gccgcggcaa acggaatcgc cagcaggttg tagccaaagg cccagagcag attctgtcgg
     2701 atcacccgac tgcttcggtc ggccaccacg aaggcggcca ccagcgacga cagtcggccg
     2761 ttcaggatga tggcgtcggc cgaactcttg gcgaggtcgc tgccttcatc gatggccacc
     2821 gccaagttgg cgccggcaag cactggcgca tcattgacgc catcgcccac catcaacacc
     2881 ttggcaccac tctgctggcg cgctctgacg gccgccagct tggcctccgg cgtcatgctg
     2941 gcctgccagt cgaccagatt cagtgaggcg gccaggggtg caaccgcagc cgggacatcg
     3001 ccactcatca aggcgagatg caagccccgt gaggccagtt gccgcagact tgcggccgca
     3061 tcacggcgtt gcggctcttc cagttcgaac caggccagca gttgcgactc gctggccaac
     3121 gcaagccact tgccatccgc cgaaggcggc gtcaccggct gctctgaccg caacagagcc
     3181 cggatgaact ccggcgtgcc gatgcgaacg ctctcgcccc gccatacacc ttcaacgccc
     3241 tgcccggcac tcgagcacag ctcaatcacc cgtgaaaccg gcaacgcacg gctggccgct
     3301 gcatcgatca gggtggcggc aatcggatgt cgcgatgcac cttccagggc cgcggccaat
     3361 gccaggcagc catcggcatc gagtccggca gcaggcaggg tcctgagcaa ccgcaggcgt
     3421 tgctcggtca gggtgccggt cttgtcgaaa accacctgat cgatctgatt caggctctcg
     3481 atcacatgac ccttgatcgc cagaaaaccc tgcttctgca gagcaagggt cgctgccgtc
     3541 aacacggtcg gcgtggccag agacagcgca cagggacagg tgatcaccag caccgaaagc
     3601 gtgatccaga aagcatccgc cgacccctgc caccaccaga agagatagac cgatgcggcg
     3661 caaccgagca tgacggtgac gaagtggctg gcgatgcgat cggtcaactg gatgatgtgc
     3721 ggcttgtcag cctgcgcctg ctgcagcagt cgttcgatca tcgccaggcg ggagtgctcg
     3781 accccgcaac tgacgctgat ccacagcggc tgctccagat tgatcgaacc accgagcagc
     3841 cgatcaccca cttcgcgaag caccggcaga cgttcaccag tcaacagcga ttcatcgaca
     3901 ctgctgcggc cgttttgcac cacgccatcg gtcgggatca cctcaccggg ccgaatcagc
     3961 agcagatcgc ccacttgcag ccgtcgcagt ggcacggact cttcaccttt ctcgcacacc
     4021 cgggttgcca accgtggcag gcgactgcgc agatcgagcg ccgccagacc cgcacgctca
     4081 cgcacggtgc gctccagaaa gcggcccagc agcagaaaaa agacgaacat caccaccgag
     4141 tcgaagtaga ccgccggccc ctgagtgaag gtggcccaga cactgaccag aaaagccagt
     4201 gcgatggcca gtgcgaccgg cacatccatg ccggggcgac gctggcgcag atcacgccag
     4261 gcaccgctga aaaagggccg ggaagagatc gccaccaccg gcagggtcag cagcaggctg
     4321 gcccagcgca gtaacgtctg atagttgcgc tcgaattcaa gaaaatcgtc gagatagagc
     4381 gcgccgctgt acatcatcac ctgcatcatg ccgatgccgg caaaacccag cccgatcagc
     4441 agatcgcgac gctcccgctg cagcaactgc tcttttgcgg tgacagtgaa aggttcggca
     4501 cgatagccca gccgatgcag cgtggcgacg atctcgccaa gccgaatctg gtcagcctgc
     4561 cagttcacct cggcgcgtcg ctggtcaaaa ttgacgctga tactgcggat gccggattgc
     4621 gccttcagac cggattcgat cagccagacg caggcggcgc aattcattcc cgccagcagc
     4681 aatgaaacat ggtggctgcc atcctccgcc acctgtacca gttcagccgc caccgccggg
     4741 tggtcatact cgctgaagtc gggcaggccg gacagcgcgg gatcatcggg attcagggct
     4801 ggtcggtctc gcaaccggta gaagcggtcc aaccctcctt cgacaatgct gcgcgcaacc
     4861 gcggcgcagc cgggacagca cattggccgc aatgtgccct cgatctccac ctgcagcgga
     4921 tcgggctgcg caatcggcag gtcacagtgg tagcacttca tcccggcttg aatgcagccc
     4981 gcaattcaac cggtcgaggc agctcggcct cccctttgag tcgccagtca cccctgtcca
     5041 aggttgccgg ggcaacagag aggtgaaaac ggccatgggg tggccgctcg agcagggtgc
     5101 gatagtgacg cggcgccacc tgtaccagcg tgaagcgctg atcctgttcg gccagcgtgg
     5161 gatggtccag ttcgagcgtc aatgcactcg gccagtcggt gagttcaccc tccaggacca
     5221 ccagaagttc cctcgcatcg gcggacagct cgatgcgggc tgacaaccgg gcggcggccg
     5281 cggcctgatc gcgccggatc gactggttga tcatcatccc ctctttgtaa tagtcgtccc
     5341 gcaccaggct gtcacggctg tcgagcgcga agccgagcag gatcagcccc gccaccaccg
     5401 acagcagcgg cagcgcgatg aggaaccagg gccagaactg acgataccat ggagcaggaa
     5461 gggttgacgt ggcagacgac atgatggaca ccctccttca gcgttgcgca gggccaatga
     5521 atcgactctc ctgagtgatt cgataatcat cacggtcgat ggcctccagc gagaagagaa
     5581 tcttgcgcga gccctgaccc agctgctgcg ccggcactgt gagtcgcagc ggcagtgaga
     5641 gcacctctga tccattcacc tcggcttcgg tggcgccctg gattttcaag ccatcgatgc
     5701 cggtggccgt gatccggtag cggtgcgggg cactgtccat gttgagaatc ttgagggtat
     5761 agctgttctc gatgccgcca tcactgctca tggtgaacag ttgattgcgg tcacggatga
     5821 tgtcgagttc gagcgggatg cgctgataga gccggtaacc gaacagcccg atcatcacca
     5881 ggatcgccac gccatagccg accagccgtg gccgcaggat atgccttttg ccggtttcga
     5941 gttcggccag cgtcgtgtag cggatcaacc cgcgcgggta gcccatcttg tccatcactt
     6001 cattgcagcc atcgatgcag agtgcacagc cgatgcactc atactgcagg ccatcgcgga
     6061 tatcgatgcc ggtcggacag acctgcacgc agaccgtgca gttgatgcag tccccaagtt
     6121 cctgtttttc ggctgcgtca ccgcgcttgc gcgaaccacg cggttcgccc cgtgccgcat
     6181 cgtaggaaat gatcagggtg tcccggtcga acatcgccga ctggaagcgt gcatagggac
     6241 acatgtattt gcagacctgc tcgcgcatcc agccggcatt gacataggtg gccagcatga
     6301 aaaacagcgt ccagaaggcc gccaccggat gggcctgcaa tagcaccaga tcgggcagca
     6361 gttcacgaat cggcgtgaag tagccgacaa aggtcaggcc ggtccagaag ccgaagccga
     6421 gccacatggc atgcttgagg cccttcttga acagcttttc ggctgaccag ggggcctgat
     6481 ccagcttgat acgctggttg cgtgaacctt cggcaaactg ctccatccac atgtagatgg
     6541 cagtccagac cgtctgcgga caggtatagc cacaccagac ccgcccaagc cagttggtga
     6601 cgaaaaacag gccaaaggca cagatgatca gcagccaggc cagaaagacg aaatcctgcg
     6661 gccagaaggt ccagttcagg atgtagaact tgcgctcggg cagactccac agcagcgact
     6721 gatgcccatt ccagttgatc catggcagca ggaagtaacc cagcagcagc ggccagccgg
     6781 tgtaaagccg aatgcgctga aagaaacctt cgatcttgcg ggtgtagatc ttctcgcgtt
     6841 tctcatacag gtcgagctcc cgggaatcgg tcaccggagg ctgtttcgga tcaatctcgc
     6901 gaaccggaag acgttgaggc atgactgtcc cgcggcaggg tgaggtcaga gtgtacaaca
     6961 gtacgatttg ttcgagtcat cccggtgatg gtgaccatgc ggccatcctc accgggatgt
     7021 cgaactcaga ttgaagtcgc tttaaaccgc tacttcttcg gttgggagag cgagtagaca
     7081 taagcagcga cgatgcggac cttctgcgca cccagcgtct gctcgaaggt cggcatctgt
     7141 ccggcccggc cggcagcgat gctgtgctcg atctgccccg gcgcgccgcc ataaagccag
     7201 atggcattgg tcagatcagg agcaccgatc agctgattgc ccttgccatc catgccgtga
     7261 caggccgtgc aggtcgaagc gaagagcgct ttgcccgcct ctgccagttt cggatcggca
     7321 gtgccaccgc tgagattgac cacatgcgcg gtcacctgct tgaccttttc atcgcccaat
     7381 atgggccccc acggcggcat ctgaccacgg cggccgctca gaatcgtggt ctcgatcgcc
     7441 tcggcagtgc caccataaag ccactcgctg tcggtcagat tgggaaaacc gaagctgccg
     7501 gttgccgatg aaccgtgaca gaccgagcag ttggtggcga agatgcgctg tcccatcttc
     7561 agcgcctgcg gctcctgctg caactgctcg accgacatcg caccgtaacg gacaaacagc
     7621 ggaccaaact ccttctcggc ctcctgcacc tctttctgcc aggcacccgt ggaactccag
     7681 ttcagcacac cgggaaagtt gccaaacccc gggtagagca ccagataggc caccagaaac
     7741 agcagcgaga gaatgaacat catgtaccac cagtacggca gcggattgtt cagctcttcg
     7801 atgccatccc actcatgacc ggtcgttgcc ccctcgcccg gcggcacctt gcggttggat
     7861 tgcagcaacc accagctccc caccgcggtc accgcgacca gcaccatcac ccacaggctc
     7921 cagaaggtac tcattgaccc ttacctcttg aattcggagt tgtttcagct tcatcgtcga
     7981 acggcagttg cgccgcttcg tcgaaggcag atttgcgttt gctgctgtaa gcccacagac
     8041 agatggcgac aaacagcagc atgaccacca ccgtgctgac ggcacggacc gtattgatct
     8101 ccatgcgcta tttcaccgat tgcatcactg tgccgagttg ctgcaggtac gcgaccatgg
     8161 catcggcttc cgatctgctc tgcagggcct gcggcgccgc ctcgatctcg gcatcgctgt
     8221 aaggaacacc cacggtgcgc aacgcccgca tcttggcctg gatctgactg gcatcgacct
     8281 cgctgtcgaa cagccaagtg aaggcaggca tgttcgactc gggcacgaca tcacgcgggt
     8341 tgtagaggtg tgcccggtgc cagtcatcgc tgtagcgacc accgacgcgg gcaaggtccg
     8401 gtccggttcg cttcgatccc cacaggaagg gatgctcata gacctgttca ccggccaccg
     8461 tatagtgacc atagcgctcg gtctcgctgc ggaaagggcg caccatctgg gtgtggcaga
     8521 catgacagcc ttcacggatg tagatgtcgc gccctgccag ctccagcggc ttcagcggtg
     8581 tcaggccggc cactggctgg gtgttgccct gtatgaaaaa aagaggggga atctccgcca
     8641 acccaccaaa actgatgacg atcgcaacca gcacgatcat cactcccagg tttttctcga
     8701 tcaggtcatg tttcatgatg cggctcccgt caaccggctt ggggaatggc gtgggtaatg
     8761 gatgcatcgg cagggcgcgc ggtcttccag acgttgtagg ccatcaccaa catcccgaag
     8821 aagaagatcg caccaccgac gaaacgcacc acatagccgg gataactcgc ctcgaccgct
     8881 tcagcgaagc tgtaagtgag ggtgccatcg gtgttgaccg cacgccacat cagcccctgc
     8941 atgatgccgt tcacccacat cgaggcgatg tagagcacgg tgccgatggt cgagagccag
     9001 aagtggaagt tgatcagcgg aatgctgaac atctcgccct gcttcttgcc gaacatgatc
     9061 ggaatcatgt ggtagagcga gccgatcgag atcatcgcca cccagcccag cgcacccgag
     9121 tggacatggc cgatggtcca gtcggtgttg tgcgacagcg cattgacggt cttgatcgac
     9181 atcatcggtc cttcgaaggt cgacatgcca tagaaagaga gcgagacgat caggaacttg
     9241 atgatcgggt cggtacgcag cttctcccac gcgcccgaga gggtcatgat gccgttgatc
     9301 atgccccccc atgacggtgc caacagcacc agtgacatca ccatgccgac gctctgcgcc
     9361 cagtcaggca gggcgctgta gtgcaggtgg tggggccccg cccacatgta gacgcagatc
     9421 agcgcccaga aatgcacgat cgacagccga taggagtaga ccggacgctc ggcctgcttg
     9481 ggaatgaagt agtacatcat cccaagaaaa ccggccgtca gataaaagcc gaccgcattg
     9541 tggccatacc accactgcac catcgcatcg atggtgccgg agtagaccga atagga
//
LOCUS       DAORCQ010000088         9469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5018_length_9469_cov_5.548438, whole genome shotgun sequence.
ACCESSION   DAORCQ010000088 DAORCQ010000000
VERSION     DAORCQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5018_length_9469_cov_5.548438"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKI23_09125"
     CDS             complement(<1..377)
                     /locus_tag="PKI23_09125"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNN37138.1"
                     /translation="MHPNKRVHGGFTLIELVILMVAIALMATIAIPSFDSSVSRNSID
                     AAQSELQAALNLARSEAVKRGKTVTLCASSDQQVCSNVWSSGWLLFVDNNSNNAVNSG
                     EEILRVGRAVNGRNGLAMSGSVS"
     gene            650..1639
                     /locus_tag="PKI23_09130"
     CDS             650..1639
                     /locus_tag="PKI23_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37139.1"
                     /translation="MKEINALTHSSTEQAPWLFDWLLSHDPSSDTAHDWPVSMASWWE
                     KYLHSATLFPATALRPIDRAIIGGALAGRVAHAFCSGYHNALQRLFPGLDDQRRAALL
                     VTERGGKSPRDIATRLTAHQEHWLLNGEKSYVSGGSTAQQLLVLAASGETAEGRRQLK
                     IVRLSSQQAGVTIEDLPAAAWMPEFRHGHARLQQVVVRAEDLLPGDGFADYTRPFATL
                     ESSFIFAALASHLFRLATVHRWPLAQRQSLLFLIMAYRSISDLDPRQPATEILLQGCH
                     DQAVAIIEQCAPCLGQLPGELSAAWQRDSGILRMAKGTLQRSAKAWERFGHSS"
     gene            1808..>2173
                     /locus_tag="PKI23_09135"
     CDS             1808..>2173
                     /locus_tag="PKI23_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNN37140.1"
                     /translation="MHKIVPRAFTALLTCSALMAGQAALAAEPPRDYVNNCAVCHGMA
                     GKGDGQLAANLTPKPTDLTQISKNNGGKFPYWRIRQSIDGRIAEGANIRGHGPKEMPV
                     WGQVFYYDSHESETVAKARM"
     assembly_gap    2174..2273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2274..3086
                     /locus_tag="PKI23_09140"
     CDS             <2274..3086
                     /locus_tag="PKI23_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNN37141.1"
                     /translation="EKIDLLVLPELHNGPYFCVSEEAAQFERAEPIPGPSTTALGQLA
                     RELNAVVIGSLFERRAAGLYHNSAVVLERDGSLAGCYRKMHIPDDPGYYEKYYFTPGD
                     LPHNRPEQAFRPIQTSAGLLGVMVCWDQWYPEAARLMALAGAELLIYPTAIGWDPDDS
                     TDEQRRQRDAWITVQRGHAIANGLPLVAVNRVGHEVDPSGATAGSRFWGSSFACGPQG
                     ELLWQAGSDETEIKLIGIDFGRSEQVRRSWPFLRDRRIDGYGDLVRRWRDPQ"
     gene            3126..4238
                     /locus_tag="PKI23_09145"
     CDS             3126..4238
                     /locus_tag="PKI23_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNN37142.1"
                     /translation="MLLILRPDIDPASSDYQQMLAHLHKLPDIRLQTHQIQGQQQTLT
                     EIYLLGNTARLDRAEIESLAAVERVVRISDEYRILGRHRDRDRVSGFHYNGVDFSQEG
                     FQLFAGLCAVDNRRNVETMMKALHEHGLECTRMGAYKPRTSPYAFQGHGADCLPDLFE
                     LAGKYGIKVIAMEVTHEAQVEEIDQTLERLGRPTGVMLQIGTRNTQNFELLKALGRQQ
                     RYPILLKRGFGITLNESLNAAEYLASEGNSNVIFCLRGMKSEYGAPHRNLVDFSQVPV
                     VKRLTRMPVCIDPSHSVGSRDSDPDGLLDLCHATAQGLIAGANMVLVDFHPQPAEALV
                     DGPQALRLEELPNYLADIALCRQSYLQRKALWSQSA"
     gene            4235..6253
                     /gene="uvrB"
                     /locus_tag="PKI23_09150"
     CDS             4235..6253
                     /gene="uvrB"
                     /locus_tag="PKI23_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNN37143.1"
                     /translation="MSGSGRFELHAGYAPAGDQPAAIAALIEGLEDGLAYQTLLGVTG
                     SGKTFTIANVVAQVQRPTLVLAHNKTLAAQLYGEFREFFPRNAVEYFVSYYDYYQPEA
                     YVPSSDTYIEKDSSVNDHIEQMRLSATKALMEREDVLVVATVSAIYGLGDPAAYLKMV
                     LHLDRGDRIDQRMLLRRLAELQYSRNDAELRRATYRVRGDVIDIHPADSEIEAVRVEL
                     FDDTIERITLFDPLTGEQLRQLPRATIFPKSHYVTPRETMVEMMEQVKVELAERLALL
                     RGQNRLVEAQRLEQRTQFDLEMISELGYCTGIENYSRYLSGRQPGEPPPTLFDYLPAN
                     ALLVIDESHVTIPQLGAMYRGDRARKETLVQYGFRLPSALDNRPLRFDEWERLSPQTL
                     FISATPSVYEAEHSARVVEQLVRPTGLIDPEIEVRPAANQVDDLLSQIRQRVNQRQRV
                     LVTTLTKRMAEDLTDYLGEHGVKVRYLHSDIDTVERVEIIRDLRLGEFDVLVGINLLR
                     EGLDMPEVALVAILDADKEGFLRSERSLIQTIGRAARNLEGKAILYADTLTGSMQRAI
                     DETERRRGVQLEFNRQHGITPRGIHKEVSDILEGAVAPGDRGRRGRKAQPAAAELQQL
                     PPQQLEKAVKKLEEQMYLHARNLEFEAAARLRDQIQALRERSYGAAQR"
     gene            6380..6712
                     /gene="grxD"
                     /locus_tag="PKI23_09155"
     CDS             6380..6712
                     /gene="grxD"
                     /locus_tag="PKI23_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNN37144.1"
                     /translation="MSDIIELIQQQIEGNPIILYMKGSPEAPQCGFSAQSSKILMACG
                     AKFAYVDVLSNPEIRANLPRHSNWPTFPQLFIDGELVGGCDIMTELYQQGELKRMVQQ
                     AIDVASGE"
     gene            6851..7273
                     /locus_tag="PKI23_09160"
     CDS             6851..7273
                     /locus_tag="PKI23_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNN37145.1"
                     /translation="MTALKGSKTESNLKAAFAGESQANRRYLYFAAKADVEGYNDVAA
                     VFRSTAEGETGHAHGHLEYLEKVGDPATGLPIGPTGDNLKAAIAGETHEYTDMYPGMA
                     KAAREEGFDEIADWFETLAKAERSHANRFQKALDTLGN"
     gene            7352..8680
                     /locus_tag="PKI23_09165"
     CDS             7352..8680
                     /locus_tag="PKI23_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNN37146.1"
                     /translation="MSREGSLEAPTRHPLDWENPQFHDRAQLDEELERVFDICHGCRR
                     CVSLCNSFPTLFDLVDESLTMEVDGVAKADYAKVVEQCYLCDLCFMTKCPYVPPHEWA
                     IDFPHLMLRAKATAFQEQGTSLRNRVLASTDQVGALASLPLVAEVVNVVNRSDLGRKA
                     LAATLGVHAQARVPEYHSSKGRNRLDRLDGPDQPPQPAGNSSGKVALFVTCYGNYNAP
                     QLVEDLARVLIHNGITVTRVRQERCCGMPKLELGDLQSVAQAKEQNIPQLLELVEDGW
                     DLIAPVPSCVLMFKQELPLMFPHDAAVARVKAAFFDPFEYLLERHRYGLLRLDFKQSL
                     GRVLYHAACHQRVQNIGPKTRDLLQLIPGTEVQSIERCSGHDGTYGVKAEYFEFSKKI
                     CRPIVRELDERAPDHYGSDCPMAGAHIENFSEGDKATEHPLSLLRLAYGI"
     gene            8718..>9469
                     /locus_tag="PKI23_09170"
     CDS             8718..>9469
                     /locus_tag="PKI23_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN37147.1"
                     /translation="MELHLAGKVAIVTGASRGIGRAIAEQLAAEGVQLVLAARSAETL
                     QQVATACNGQAVIFPADLRDPQAPQRLIDFAIERFGTIDLLVNNAGATRRGDFLQLSE
                     ADWEDGFALKFFSTVRCSRAAWPHLVRSTGAIVNIVGVGGRTGDAEFAIGGAVNAALL
                     NLTKVLAERGMAEGVKVNAINPGLIATDRLQARIDQLAAIHNLDPAAAATAMARNLGI
                     PRIGQPAEIAAAVAFLASSASAYCQGSILDIDG"
BASE COUNT         1767 a         3184 c         2858 g         1560 t
ORIGIN      
        1 ttcgagaccg atccgctcat cgccaggccg ttgcggccat tcaccgcccg gccgacccgc
       61 aggatctcct cgccgctgtt gaccgcgttg ttggagttgt tgtcgacgaa cagcaaccag
      121 ccactgctcc agacattgct gcaaacctgc tggtcgctgc tggcgcagag ggtgaccgtc
      181 ttgccgcgct tgaccgcctc gctgcgcgcc aggttgagcg ccgcctgcaa ctcgctttgc
      241 gcggcgtcga tgctgttgcg tgagacactc gaatcgaaac tggggatggc gatggtggcc
      301 atcagcgcga tggccaccat caggatcacc agctcgatca gggtaaaacc gccgtgaact
      361 ctcttgttcg gatgcatggc cagatcagca gtggtggatg tctgatcaaa agatagatga
      421 accgccaccg cgtgccggtg agccgcgatg aaaggtggca tgcagcggat gaacggcggc
      481 ccggcggctt cgggtcgtgc aggccgtgac ccgggtcaca ctctgccggt ccgctgcaac
      541 ggcagcgccg atcggatcgc acggctggcc atcggcaccg caggctgttc acagcccggc
      601 cgacgcgtga caatggtgct tcgaacactc cggggacgga tcaaaatcaa tgaaagaaat
      661 caatgcattg acacactcat caacagagca ggctccctgg ctgttcgact ggctgctgag
      721 tcatgaccca tccagcgaca ctgcgcacga ctggccggtc agcatggcgt catggtggga
      781 gaagtatctg cacagtgcga cgctgttccc cgccacagcg ctcagaccga tcgaccgcgc
      841 catcatcggc ggcgcgctgg ccggccgggt ggcccatgcc ttctgcagcg gctatcacaa
      901 tgcactgcag cggcttttcc ccggtctcga cgaccagcgc cgcgccgcgc tgctggtcac
      961 cgagcgcggt ggcaagagcc cgcgcgacat cgccacccgc ctgaccgccc atcaggagca
     1021 ctggctgctc aatggcgaga agagctatgt ctccggtggc agcactgccc agcaactgct
     1081 ggtgctggcg gccagcggcg agacggccga aggccggcgg cagctcaaga tcgtgcgact
     1141 gtcgagccag caggcgggcg tcacgatcga ggatctgccg gccgctgcct ggatgccgga
     1201 gttcagacat ggccatgccc gcctgcagca ggtcgtggtg cgcgcggagg atctcctgcc
     1261 gggcgatggc ttcgccgact acacgcgccc gttcgccacc ctcgaatcga gcttcatctt
     1321 cgcggcactc gccagccatc tgtttcgcct tgccaccgtc caccgctggc cgctggcgca
     1381 gcgccagtcg ctgctgttcc tgatcatggc ctaccgcagc atcagcgacc tcgatccacg
     1441 ccagcctgcg accgaaatcc tgctgcaggg ctgccatgac caggccgtcg ccatcatcga
     1501 acagtgtgcg ccctgcctcg gccaactgcc cggcgaactg tcagcggcct ggcagcgcga
     1561 cagtggcatt ctgcggatgg ccaagggcac cctgcagcgc tcggccaagg cctgggagag
     1621 gttcggccac tcatcatgaa ccgtcggacg caagcgaatg agcgcttgct ttcgatcgac
     1681 gttttaataa caattccaag cacccacaga gaaactgggt gctctgactt gcagtgatga
     1741 tagggaattg tcccagccgg acagcacgac cgatggactg tccggcatca accccgagga
     1801 gaccataatg cataagatcg tcccccgcgc tttcaccgct ctgctgacct gttctgcctt
     1861 gatggcaggt caggccgccc tggcagccga gcccccccgc gactacgtca acaactgcgc
     1921 ggtgtgccac ggcatggccg gcaagggcga cgggcaactg gccgccaacc tgacgccgaa
     1981 accgaccgat ctgacccaga tcagcaagaa caatggcggc aaattcccct actggcgcat
     2041 tcgccagtcg atcgatggcc gcatcgccga aggggcgaac atccgcggcc atggtcccaa
     2101 ggagatgccg gtctggggac aggtgttcta ctacgactcg cacgagagcg aaaccgtcgc
     2161 caaggcgagg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagaaga
     2281 tcgatctgct ggtgctgccg gagctgcaca atggccccta cttctgcgtc agcgaagagg
     2341 ccgcccagtt cgagcgggcc gaaccgattc ccggaccctc gaccacggcg ctgggtcaac
     2401 tggcgcgcga actgaacgca gtcgtcatcg gctcactgtt cgagcggcgc gcggccggtc
     2461 tctaccacaa cagtgccgtg gtgctggagc gcgacggcag cctggctggc tgctaccgca
     2521 agatgcacat ccccgatgac cccggctatt acgaaaagta ctacttcacc cccggtgatc
     2581 tgccccacaa caggccggag caggcctttc ggccgatcca gaccagcgcc ggcctgctcg
     2641 gcgtcatggt ctgctgggat cagtggtacc ctgaggcagc acggctgatg gcgctggccg
     2701 gcgccgagct gctgatctac cccaccgcga ttggctggga ccccgacgac agtaccgatg
     2761 agcaacggcg gcagcgcgat gcctggatca ccgtgcagcg tggccatgcg attgccaatg
     2821 gtttgccgct ggtcgcggtg aaccgcgtcg gccatgaggt cgaccccagc ggtgccacgg
     2881 ccggatcacg cttctggggt tcgagtttcg cctgcggacc acaaggcgag ctgctgtggc
     2941 aggcgggcag tgatgagacc gaaatcaagc tgatcggcat cgacttcggt cgcagcgaac
     3001 aggtacgccg ctcttggccc tttctgcgtg accgccgcat cgacggctat ggcgacctcg
     3061 tccggcgctg gcgtgatccg cagtagtgtg cagcccgcct ccaccttcgt catcgagagc
     3121 cccccatgtt gctgatactg cgtcccgaca tcgatcccgc ctcgtcagac taccagcaga
     3181 tgctcgccca tctgcacaag ctgcctgaca tccggctgca aacccaccag attcagggtc
     3241 agcagcaaac cctgaccgag atctacctgc tcggcaacac cgcccgcctc gaccgcgccg
     3301 aaatcgagag cctggccgcg gtggagcggg tggtgcgcat ctccgatgag taccgcatcc
     3361 tcggccgcca ccgcgaccgt gaccgggtca gcggctttca ttacaacggt gtcgacttct
     3421 cgcaggaggg ctttcagctc ttcgccgggc tctgcgcggt ggacaaccgc cgcaatgtcg
     3481 agaccatgat gaaagccctg cacgaacatg gccttgaatg cacccgcatg ggcgcctaca
     3541 agccgcgcac cagcccctac gcctttcagg gccatggcgc cgactgcctg cccgatctgt
     3601 ttgaactggc gggtaaatac ggcatcaagg tgatcgccat ggaggtgacc cacgaggcgc
     3661 aggtcgagga gatcgaccag accctggagc ggctcggccg ccccactggt gtgatgttgc
     3721 agatcggcac ccgcaacacc cagaacttcg agctgctgaa ggcgctgggg cggcagcagc
     3781 gctatccgat cctgctcaag cgcggcttcg gcatcacgct gaacgagtcg ctgaatgccg
     3841 cggagtatct ggccagcgaa ggcaactcca atgtgatctt ctgcctgcgc ggcatgaaga
     3901 gcgagtatgg cgcgccacac cgcaatctgg tcgacttcag tcaggtgccg gtggtcaagc
     3961 ggctcacccg catgccggtc tgcatcgacc cgtcgcactc ggtcggcagc cgcgacagcg
     4021 accccgatgg cctgctcgat ctctgccacg ccacggcgca gggcctcatc gccggcgcca
     4081 acatggtgct ggtcgacttc catccgcagc cggccgaggc actggtcgat ggcccgcagg
     4141 cgctgcggct cgaagagctg cccaactacc tggccgacat cgcactctgt cgacagagct
     4201 acctgcaacg caaggcgctc tggagccagt cggcttgagc ggctcaggtc ggttcgaact
     4261 gcacgccggc tatgcgccgg ccggggacca gccggcggcc atcgcggcgc tgatcgaagg
     4321 gctggaggat ggcctggcct accagacgct gctgggcgtc accggctcgg gcaagacctt
     4381 caccatcgcc aacgtggtgg cgcaggtgca gcggccgacc ctggtgctgg cgcacaacaa
     4441 gacactggcg gcgcaactct atggcgagtt ccgcgagttc tttccccgca acgcggtgga
     4501 gtatttcgtc tcctactacg actattacca gcccgaagcc tacgtcccct cctccgacac
     4561 ctacatcgag aaagactcct cggtcaacga ccacatcgag cagatgcggc tgtcggccac
     4621 caaggcgctg atggagcgtg aggatgtgct ggtggtggcc accgtgtcgg cgatctacgg
     4681 cctcggcgac ccggccgcct acctcaagat ggtgctgcac ctcgaccgtg gcgaccggat
     4741 cgatcagcgg atgctgctgc gccgcttggc cgagctgcaa tacagccgca acgatgccga
     4801 actgcgccgc gccacctacc gggtgcgcgg cgatgtgatc gacatccatc cggccgactc
     4861 cgaaatcgag gcggtgcggg tcgagctgtt cgacgacacc atcgagcgca tcacgctgtt
     4921 cgacccgttg accggcgagc aactgcggca gttgccacgc gcgaccatct ttcccaagag
     4981 ccactacgtc acgccacgcg aaaccatggt cgagatgatg gagcaggtga aggtcgagct
     5041 ggccgagcgt ctggcgctgc tgcgcggcca gaaccgtctg gtcgaggcgc agcggctcga
     5101 acagcgcacc cagttcgacc tggagatgat cagcgaactg ggctactgca ccggcatcga
     5161 gaactactcc cgctacctct ccggccgcca gccgggagag ccaccgccca ccctgttcga
     5221 ctatctgccg gccaacgccc tgctggtaat cgatgaatcc cacgtcacca ttccacagct
     5281 tggcgccatg taccgcggcg accgggcgcg caaggagacg ctggtgcagt acggttttcg
     5341 cctgccctcg gcgctcgaca accgtccgct gcgctttgac gaatgggagc ggctctcgcc
     5401 gcagaccctc ttcatctcgg ccaccccgtc ggtctacgaa gcggaacaca gcgccagggt
     5461 ggtggagcaa ctggtgcgtc ccaccggtct catcgacccc gaaatcgagg tgcgtcccgc
     5521 cgccaaccag gtcgatgacc tgctgtcgca gattcggcag cgcgtcaacc agcggcagcg
     5581 cgtgctggtc accaccctga ccaagcggat ggccgaggac ctcaccgact acctcggcga
     5641 acatggcgtc aaggtgcgct atctgcactc cgacatcgac accgtcgaac gggtcgagat
     5701 catccgcgat ctgcgccttg gcgagttcga tgtgctggtc ggcatcaacc tgctgcgcga
     5761 ggggctcgac atgcccgaag tggcgctggt cgccatcctc gatgccgaca aggagggctt
     5821 tctgcgctcc gaacgctcac tgatccagac catcggccgg gcggcgcgca acctcgaagg
     5881 caaggcgatt ctctatgccg acaccctcac cggttcgatg cagcgtgcga tcgacgagac
     5941 cgaacggcgc cgcggcgtgc agctcgaatt caaccgtcag catggcatca caccgcgcgg
     6001 catccacaaa gaggtcagcg atatcctcga aggggcagtg gcgcccggcg accgcggccg
     6061 gcgtggccgc aaggcccagc cagcggccgc cgagttgcag caactgccgc cacaacagtt
     6121 ggagaaggcc gtgaagaaac tggaagagca gatgtatctg catgccagaa atctcgaatt
     6181 cgaggccgcg gcacggctgc gcgaccagat tcaggcgctg cgcgagcgca gttatggggc
     6241 tgcgcagcgg tagcgtcgtc catatatccc cagccactgg cgtgataatc tttttccgtc
     6301 cgggattaca ctgtcccctc cagttaccca ggcgcacgtt gcgccaccac ccacttcttg
     6361 ccccgaggag cagcacacca tgagcgacat catcgaactg attcaacagc agatcgaagg
     6421 caacccgatc atcctctaca tgaagggctc gcccgaagcg ccccagtgcg gcttctcggc
     6481 ccagtcatcg aagatcctga tggcctgcgg cgcgaagttc gcctatgtcg atgtgctctc
     6541 caacccggag atccgcgcca acctgccgcg ccactccaac tggccgacct tcccgcagct
     6601 cttcatcgat ggcgagctgg tgggcggttg cgacatcatg accgagctct accagcaggg
     6661 cgaactgaag cggatggtgc aacaggcgat cgatgtcgct tccggtgagt gaaccgcccg
     6721 ggttccaccc gctcaatcac gctggccaaa tggccagcgc tcgcttagag tgcgaccacg
     6781 gagtcggcgc cgggaacccc accggcacca ttccagcccg caaaccacga ccacccatga
     6841 gaggctgatc atgactgcac tgaaaggaag taaaaccgaa agcaacctca aagccgcctt
     6901 tgccggggag tcccaggcca atcgccgcta tctctacttt gccgccaagg ccgacgtcga
     6961 gggctacaac gacgtggcgg cggtctttcg ctccaccgca gagggtgaaa ccggccatgc
     7021 ccatggccac ctcgaatatc tggagaaagt cggcgatccg gccaccgggc tgccaatcgg
     7081 tccgaccggc gacaacctga aggcggccat cgccggtgag acccacgagt acaccgacat
     7141 gtatcccggc atggccaagg ccgcccgcga agagggcttc gacgagatcg ccgactggtt
     7201 cgagacgctg gccaaggccg agcgctcgca cgccaaccgc ttccagaagg cactcgacac
     7261 cctcggcaac tgagccgcgg cagcccatct gtcgatcaca agcgggaact tcggttcccg
     7321 ctttcggttt acccgtcaca ggagatgacc catgtcccgc gaaggcagcc tcgaagcccc
     7381 cacccgccat ccgctcgact gggaaaatcc gcaattccat gaccgcgcgc agctcgatga
     7441 ggagctggag cgggtcttcg acatctgcca tggctgtcgc cgctgcgtca gcctctgcaa
     7501 cagctttccg accctgttcg atctggtgga cgagtcgctg acgatggagg tcgatggtgt
     7561 cgccaaggcg gactatgcca aggtggtcga gcagtgctac ctctgcgacc tctgcttcat
     7621 gaccaagtgc ccctatgtac cgccgcatga gtgggccatc gactttcccc acctgatgct
     7681 gcgcgccaag gccacggcat tccaggagca gggcacctcg ctgcgcaacc gggtgctggc
     7741 cagcaccgac caggtcggcg ccctggccag ccttccgctg gtggccgagg tggtcaacgt
     7801 cgttaaccgc agcgacctgg gtcgcaaggc gctggcggcc acgctgggcg tgcatgcgca
     7861 ggcgcgggtg cccgaatacc attccagcaa gggccgcaac cgtctcgatc ggcttgacgg
     7921 ccccgaccaa ccaccccaac ctgccggcaa cagcagtggt aaggtcgcgc tcttcgtcac
     7981 ctgctacggc aactacaatg caccgcaact ggtcgaggat ctggcccggg tgctgattca
     8041 caacggcatc accgtgaccc gggtgcggca ggagcgctgc tgcggcatgc cgaagctcga
     8101 actgggtgac ctgcaatcgg tggcgcaggc caaggagcag aacatcccgc agcttctcga
     8161 actggtcgag gatggctggg acctcatcgc gccggtcccc tcctgtgttc tgatgttcaa
     8221 gcaggagttg ccactgatgt tcccccatga cgccgccgtt gcgcgggtca aggcggcctt
     8281 tttcgatccg ttcgaatacc tgctcgaacg gcaccgttat ggcctgcttc ggctcgactt
     8341 caaacagtca ctcggccggg tgctctacca tgccgcctgc caccagcggg tgcagaacat
     8401 cggccccaag acccgtgacc tgctgcaact gattcccggc accgaggtgc agagcatcga
     8461 acgctgctcc ggccacgatg gcacctatgg agtcaaggcg gaatatttcg agttctcgaa
     8521 gaaaatctgt cgaccgatcg tccgcgaact ggatgaacgc gcacccgacc actatggcag
     8581 cgactgtccg atggccggcg cccacatcga aaatttcagc gagggtgaca aggcgaccga
     8641 gcatccactc tcgctgctgc ggctggccta cggcatctag acacgcaacc tccactcacc
     8701 gactccgcga ggccgacatg gagctgcatc tcgccggcaa ggtggccatc gtcaccggcg
     8761 ccagccgtgg catcgggcgc gccattgccg aacagctcgc cgctgaagga gtgcaactgg
     8821 tattggcggc ccgttcggcg gagacgctgc aacaggtcgc caccgcctgc aacggccagg
     8881 cggtgatctt tccggccgat ctgcgtgatc cccaggcacc acagcggttg atcgattttg
     8941 cgatcgagcg gttcggcacc atcgacctgc tggtcaacaa cgccggcgcc acccgtcgcg
     9001 gtgacttcct gcagctcagc gaagcagact gggaggatgg cttcgcgctg aagttcttca
     9061 gcacggtccg ttgctcccga gccgcctggc cgcatctggt ccgctcgacg ggcgccatcg
     9121 tcaacatcgt cggcgtcggt ggccgcaccg gcgatgccga gttcgcgatc ggcggcgcgg
     9181 tcaatgcggc gctgctgaac ctcaccaagg tgctggccga gcgcggcatg gccgaggggg
     9241 tcaaggtcaa tgcgatcaac ccagggctga tcgccaccga ccgcctgcag gcacgaatcg
     9301 atcaactggc tgcgattcac aacctcgatc cggcggcagc cgccacggca atggccagaa
     9361 acctgggcat cccgcgcatc ggccagccgg ccgaaattgc cgctgcggtg gcctttctgg
     9421 cctcttcagc ctccgcctac tgccagggtt cgatcctcga catcgacgg
//
LOCUS       DAORCQ010000089         9325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5143_length_9325_cov_5.877994, whole genome shotgun sequence.
ACCESSION   DAORCQ010000089 DAORCQ010000000
VERSION     DAORCQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5143_length_9325_cov_5.877994"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="PKI23_09175"
     CDS             <1..1442
                     /locus_tag="PKI23_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNN37148.1"
                     /translation="GLVIHPPMLYMGYVGFSVAFAFAIAALTSGRLDAAWARWSRPWT
                     AVAWLFLTLGITLGSWWAYYELGWGGWWFWDPVENASFMPWLIGTALIHSLAATEKRG
                     VFKSWTVLLAIFAFSLSLLGTFLVRSGVLTSVHAFAVDPQRGMFILLFLLLVIGGSLT
                     LYAMRAPTVASQTRFELWSRESFLLFNNLLLTVACASVLIGTLYPLAYEALNAGRKIS
                     VGPPYFNAVFIPLMGLLILFMGAGPLLRWRATDRSELLRRLLWMALIALPLALLLPWL
                     LVRALIPSTAIAALLAGWIGAGLLLDLIIKGRQLHAQGGLRRLPISHWGMVAAHLGVA
                     VTALGIALVSHHSSERDLRLKPGESAELAGHRFQFKGVSELAGPNYLASHGEVEVSRA
                     GHPIATLAPQKRLYQARGNSMTEAAIDPGFTRDLYIALGEPLENGAWALRIQYKAYVR
                     WIWLGGLLMVAGALLTLCDRRYRTTGATP"
     gene            1439..1972
                     /locus_tag="PKI23_09180"
     CDS             1439..1972
                     /locus_tag="PKI23_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNN37149.1"
                     /translation="MTRWKLFIPLLLFLALGAFLLRGLFMDPTLLPSPLIGKPLPAFT
                     LGTLADPAGVKRQSDLTGEVALVNVWATWCISCRAEHPWLMKIAAMGVPIYGVNYKDQ
                     REAALTWLKQLGDPYRFSLFDESGRLGLDLGVYGAPESYLIDRHGVIRYKRVGVIDER
                     IWRDELEPAINRLRAEP"
     gene            1969..2448
                     /locus_tag="PKI23_09185"
     CDS             1969..2448
                     /locus_tag="PKI23_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNN37150.1"
                     /translation="MSAALTRWLLLGTLLLTTTLGAVEPDYQFDQPEQSQRFHRLLDQ
                     FRCPKCQNTNLVGSDAPIAADLRQHIYQLMNEGRSDREIEEYLVERYGEFVLYKPRAQ
                     GSNWLLWLAPAMLLLGGLGYVALLIARRRPQATPATGLSSDERARLDQLLKRQEPPG"
     gene            2448..3683
                     /gene="ccmI"
                     /locus_tag="PKI23_09190"
     CDS             2448..3683
                     /gene="ccmI"
                     /locus_tag="PKI23_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNN37151.1"
                     /translation="MPLFALAIALLICLALAFILLPLRRATAAQVSEASRQQTNLALH
                     RRRLDDLQQEHRRGEIDDAALAALTLELERDLLVDVDSAEPSSAGTTTPASLWSRHWL
                     VLLVAGLLPLVALLLYGRLGGIDQIELTRLSERLTQTAVESPEFPALQEQLASRLQPT
                     ANQLSGWYLLASSALQNGRLDLATDTLQQIARLNGESTDNAPVYAQLAQVAFQQAGQK
                     ITPQIEGWIQQALALDPDEPTALGLLGIAAFARSDYQAAIDAWQHALTRTPAGASSDA
                     LRSGIGVARERLGLPPEPAVAGRIEVTIEIDPALLPEVSPDSHLLLFARAANGPPMPL
                     AAVRLPVGDWPREVQLDDSMAMQPGLKLSLHPQVEVVARISRSGGAQAMAGDLEGRSG
                     VIEPSRQQGKLKLLINQRR"
     gene            3700..4728
                     /gene="mtnA"
                     /locus_tag="PKI23_09195"
     CDS             3700..4728
                     /gene="mtnA"
                     /locus_tag="PKI23_09195"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNN37152.1"
                     /translation="MQPLSCLSLRLDAEGLFLLDQTLLPQQECWHPCRDVATLEQMIK
                     TLQVRGAPMIALCCALLLAWLTEQGVPRAQIADALERLRATRPTAVNLMHAMERLRAT
                     LATDDWPQRLIGEAERLFIEDRALCTAMAGHGATLIANGDRILTHCNTGGLATAGIGT
                     AIGVIRHAHELGRNISVWVDETRPLLQGGRLTAWELGRFGIPYRLICDSMAAVLMGSG
                     QVDKVLVGADRIAANGDFANKIGTYGLAVLAHHHQIPFYVVAPHTTVDGHCPNGKVIP
                     IEQRDGAEVRGVRTAHGHLEWSPADAPVFNPAFDVTPAALVTGWVLDSGLYGQADVEQ
                     GLFGSMQG"
     gene            complement(4733..5179)
                     /locus_tag="PKI23_09200"
     CDS             complement(4733..5179)
                     /locus_tag="PKI23_09200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNN37153.1"
                     /translation="MAELPQFSNYNEATARSLIESNNRNMGGLPGFIGMELVEFGPGT
                     LRAKVEVRDELLTAFGTMHGGVMAGFIDHVLGVVLYPLMQPGQWAATTEFKLNYLASV
                     SKGTLVADSEVVSLTRTTAVVRALVTNEGRLCCAAQGTLLIREPRK"
     gene            5347..6144
                     /locus_tag="PKI23_09205"
     CDS             5347..6144
                     /locus_tag="PKI23_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016852662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNN37154.1"
                     /translation="MMRVSELYLHPVKSCGGHAVERVELDPQGPRGDRRWMVTDPTGQ
                     FLTQRELARMALIQVGVTDSGITLRAPGFDTLTVMTPPQEQASERLVTVWRDQCRALL
                     ADAAANQWLSDFLQRPAQLVHLPDSSRRPVDPRYATASDQVSFADGFPLLLIGAASLD
                     ELNSRLDQPVSMRRFRPNLVVSGGAAFAEDGWRRLRIGTITFRVAKPCARCAIPGIDP
                     DSGQTTAEPLRTLARFRRRDGVIYFGQNLLHDGPGTLTVGDPVELLE"
     gene            complement(6172..6615)
                     /locus_tag="PKI23_09210"
     CDS             complement(6172..6615)
                     /locus_tag="PKI23_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNN37155.1"
                     /translation="MADYKKILVSIDLTAEADQVVEQASSIAARYQAELSLIHVIEPL
                     TFAYGGDIPMDVSTIQEEIETQAKARLRGLGSQWNIPAERQYLIYGRPAAEVHRLVTE
                     QQADLIVIGSHGRHGIGLVLGSTATAVLHGAKCDVLAVRVRSATK"
     gene            6833..7282
                     /gene="clpS"
                     /locus_tag="PKI23_09215"
     CDS             6833..7282
                     /gene="clpS"
                     /locus_tag="PKI23_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNN37156.1"
                     /translation="MPDRSHGASHPGSVAGGKHATFPRSTLLQQRNFHLTFSGNAGGS
                     SPAGGQQGDTAIESAKPKLKPPSRYQVVMLNDDFTPMEFVVDVLQMFFGMPLEKASQI
                     MLIVHTRGRAVCGVYSRDIAETKAFQVTQYAREHQHPLLCEIERVDE"
     gene            7303..>9325
                     /gene="clpA"
                     /locus_tag="PKI23_09220"
     CDS             7303..>9325
                     /gene="clpA"
                     /locus_tag="PKI23_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNN37157.1"
                     /translation="MLSKDLEVSLNLAFQGARNKRHEFMTVEHLLLALLDNDIAIDVL
                     KACGIDLERLRSDLEEFVESTTPLLPEGDKERDTQPTLSFQRVLQRAIFHVQSSGKRE
                     VTGANVLVAIFSEQESHAIFLLKQQQVARIDVVNYVAHGIAKVSRHQEQLDPGPAAGD
                     EERSGSEGSGQHPLEAFATNLNQQAMQGRIDPLVGREHELQRVVQILARRRKNNPLLV
                     GEAGVGKTAIAEGLAKRIVEGDVPEVIANSVVYSLDLGSLLAGTKYRGDFEKRFKALL
                     AELKKQENAILFVDEIHTIIGAGAASGGVMDASNLLKPILTSGELRCIGSTTFQEYRG
                     IFDKDRALSRRFQKIDVLEPSVDETVQILKGLKSRFEAHHGLRYTAGALQAAAELSAR
                     YITDRQLPDKAIDVIDEAGAAQRLKPPSRRKKVIGTPEVEEVVASIARIPPKSVSASD
                     KEVLRNLEGNLKKLVFGQDQAIEALATAIKLSRAGLKSVDKPIGAFLLSGPTGVGKTE
                     VSRQLANILGVQLLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKNPHCVL
                     LLDEIEKAHPEVFNILLQVMDHGTLTDNNGRRADFRNVILLMTTNAGASEMSRTSIGF
                     TKQDHSSDGMEAIRRMFSPEFRNRLDAIIPFAPLSPQVIVSVVDKFLVEL"
BASE COUNT         1602 a         3199 c         2957 g         1567 t
ORIGIN      
        1 cgggtctggt gatccatccg ccgatgctct acatgggcta tgtcggcttc tcggtcgcct
       61 tcgcctttgc catcgccgcg ctgaccagcg gccggctcga tgccgcctgg gcgcgctggt
      121 cacgcccctg gactgcggtc gcctggctgt ttctgaccct tggcatcacc ctcggcagct
      181 ggtgggccta ctacgaactg ggctggggcg gctggtggtt ctgggatccg gtcgagaacg
      241 cctcgttcat gccctggctg atcggcaccg cactgatcca ctcgctggcc gccaccgaga
      301 agcgcggcgt gttcaagagc tggacggtgc tgctggcgat cttcgccttc tcgctgagtc
      361 tgctcggcac ctttctggtc cgctccggcg tcctcacttc ggtccatgcc ttcgcggtcg
      421 acccgcagcg cgggatgttc atcctgctgt tcctgctgct ggtgatcggc ggatcactga
      481 cgctctatgc catgcgcgcg ccgaccgtcg cgtcacagac gcgtttcgag ctctggtcgc
      541 gcgagagctt tctgctgttc aacaacctgc tgctgaccgt cgcctgcgcc tcggtgctga
      601 tcggcaccct ctatccgctg gcttatgaag cgctcaatgc cggccgcaag atctcggtcg
      661 ggccacccta cttcaacgcg gtgttcattc cgctgatggg gctgctgatc ctgttcatgg
      721 gcgccggtcc gctgctgcgc tggcgcgcca ccgaccgcag cgaattgctg cgccggctgc
      781 tgtggatggc gctgatcgcg ctgccgctgg cactgctgct gccctggctg ctggtgcggg
      841 cgctgatccc ttccaccgcc atcgcggcgc tgctggccgg ctggatcggt gccgggctgc
      901 tgctcgatct gatcatcaag gggcggcaac tgcacgcgca gggcggactg cggcggctgc
      961 cgatcagcca ctggggcatg gtggccgccc atctgggggt ggcagtgacc gcgctcggca
     1021 tcgcgctggt cagtcaccac agcagcgagc gtgacctgcg gctgaagcca ggcgagtcgg
     1081 ccgaactggc cggccaccgc ttccagttca agggggtcag cgagcttgcc ggacccaact
     1141 atctggccag ccatggcgaa gtcgaggtaa gccgcgccgg ccaccccatc gccacgctgg
     1201 caccgcagaa gcggctctat caggcccgcg gcaacagcat gaccgaggcg gcgatcgacc
     1261 ccggcttcac ccgcgacctc tacatcgccc tcggtgaacc actcgaaaac ggcgcctggg
     1321 cactgcgcat ccagtacaag gcctatgtgc gctggatctg gctcggtggc ctgctgatgg
     1381 tggccggtgc gctgctgacg ctctgcgacc gccgctaccg cacgacagga gccacgccat
     1441 gacgcgctgg aagctgttca tcccgctgct gctgtttctg gcgctcggcg cctttttgct
     1501 gcgcgggctg ttcatggacc cgacgctgct gccctcgccg ctgatcggca agccgctgcc
     1561 ggccttcacg ctcggcaccc tcgctgatcc ggccggcgtc aaacggcaga gcgacctgac
     1621 cggtgaggtg gcgctggtca atgtctgggc cacctggtgc atctcctgcc gcgccgaaca
     1681 cccctggctg atgaagatcg ccgcgatggg cgtgccgatc tacggcgtca actacaagga
     1741 tcagcgcgag gccgcgctga cctggctcaa gcagctcggc gacccctacc gcttcagcct
     1801 gttcgacgag agcggccggc tcgggctcga cctcggcgtc tatggcgcac ccgagagcta
     1861 cctgatcgac cgccatggcg tcatccgcta caagcgggtc ggcgtgatcg acgagcgcat
     1921 ctggcgcgac gagctggagc cagccatcaa cagactgcgg gccgagccat gagcgcggca
     1981 ctgacccgct ggctgctgct cggcacgctg ctgctgacca ccactctcgg cgccgtggag
     2041 cccgactacc agttcgacca gccggagcag agccagcgct ttcaccgcct gctcgaccag
     2101 ttccgctgcc ccaagtgcca gaataccaac ctggtcggct ccgatgcccc gatcgccgcc
     2161 gacctgcgcc agcacatcta ccagttgatg aacgaggggc gcagtgaccg ggagatcgag
     2221 gagtacctgg tcgagcgcta tggcgagttc gtgctctaca aaccacgcgc ccagggcagc
     2281 aactggctgc tgtggctggc gccggccatg ctgctgctgg gcggcctcgg ctacgtcgcc
     2341 ctgctgatcg cgcggcgtcg gccgcaggca acacccgcca ccggactgag cagcgacgaa
     2401 cgcgcccggc tcgaccaact gctgaagcga caggagccgc ccggatgatg ccgctgtttg
     2461 ccctggccat cgcgctgctg atctgcctgg cgctcgcctt catcctgctg ccactgcgcc
     2521 gcgccacggc ggcgcaggtc agcgaagcga gccgccagca gaccaatctg gccctgcatc
     2581 gccgccggct cgatgacctg caacaggagc atcgccgcgg cgagatcgac gacgctgcgc
     2641 tggcggcgct gacgctggag ctggagcgcg acctgctggt cgacgtcgac agcgccgaac
     2701 ccagctcggc cgggacgacc acgccggcca gcctctggtc gcggcactgg ctggtgctgc
     2761 tggtcgccgg cctgctgccg ttggttgcgc tgctgctcta tggccgtctg ggcggcatcg
     2821 accagatcga actgacccgg ctgagcgagc ggttgacgca gacagcagtc gaaagcccgg
     2881 agttcccggc actgcaggag cagctcgcca gccggctgca accgaccgcc aatcagctct
     2941 ccggctggta cctgctcgcc agctcggcac tgcagaacgg ccggctcgac ctggccaccg
     3001 acacactgca acagattgcc cgcctcaatg gcgaatcgac cgacaacgca ccggtctatg
     3061 cgcaactggc gcaggtcgcc ttccagcagg ccgggcagaa gatcacgccg cagatcgagg
     3121 gctggatcca gcaggcgctg gcactcgatc ccgacgagcc aaccgcgctg gggctgctcg
     3181 gcatcgccgc cttcgcccgc agcgactatc aagccgcgat cgacgcctgg cagcacgcgc
     3241 tgacccggac gccggccggc gccagcagcg atgcgctgcg cagcggcatc ggcgtcgccc
     3301 gcgaacggct ggggctgccg cccgaaccgg ccgtggccgg ccgcatcgaa gtgaccatcg
     3361 agatcgatcc ggcactgctg cccgaggtgt cgcccgacag ccacctgctg ctcttcgccc
     3421 gtgccgccaa tggcccgccg atgccgctgg cggccgtccg tctgccagtc ggcgactggc
     3481 cgcgcgaggt gcaactcgat gactcgatgg cgatgcagcc cggtctcaag ctctcgctcc
     3541 atccgcaggt cgaggtggtg gcacgcatca gccgcagcgg tggtgcccag gccatggccg
     3601 gcgatctcga aggccgctcc ggcgtgatcg aaccgtcccg gcagcagggc aagctcaagc
     3661 tgctgatcaa ccagcgtcgc tgaacagggc cctcgcacca tgcagccact ctcctgcctc
     3721 agcctgcggc tcgacgccga gggactgttc ctgctcgacc agacgctgct gccgcagcag
     3781 gagtgctggc acccctgccg cgacgtggcc acgctggagc agatgatcaa gacattgcag
     3841 gtgcgcggag cgccgatgat cgcactctgc tgcgcgctgc tgctggcctg gctgaccgaa
     3901 cagggcgtgc cgcgcgcgca gattgccgac gcgctggagc ggctgcgcgc cacccgacca
     3961 accgcggtca atctgatgca tgcgatggag cggctgcgcg ccaccctcgc caccgatgac
     4021 tggccgcagc ggctgatcgg cgaggccgaa cggctcttca tcgaggatcg ggcactctgc
     4081 acagcgatgg cgggccacgg cgcgacgctg atcgccaacg gtgaccgcat cctcacccac
     4141 tgcaacaccg gcgggctggc caccgccggc atcggcaccg cgatcggtgt catccgtcac
     4201 gctcacgagc tcggccggaa catctcggtc tgggtcgacg agacccgccc gctgctgcaa
     4261 gggggccggc tgaccgcctg ggaactgggc cgattcggca tcccctatcg gctgatctgc
     4321 gacagcatgg ccgcggtgct gatgggcagc gggcaggtcg acaaggtgct ggtcggcgcc
     4381 gaccgcatcg ccgccaatgg cgatttcgcc aacaagatcg gcacctacgg gctcgccgtt
     4441 ctggcgcacc accaccagat tccgttctat gtggtcgcgc cccacaccac cgtggatgga
     4501 cactgcccga atggcaaggt gattccgatc gaacagcgcg atggcgccga ggtgcgcggc
     4561 gtgcgcaccg ctcacggcca tctggaatgg agccccgccg acgcaccggt gttcaacccg
     4621 gcgttcgatg tcaccccggc ggcactggtg accggctggg tgctcgacag cggcctttac
     4681 ggccaggccg acgtcgagca gggtctgttc ggatcaatgc agggctgagc ggctacttgc
     4741 gcggctcgcg gatcagcagc gtaccctgtg ccgcgcagca gagccgtcct tcgttggtca
     4801 ccagcgcccg caccacggcg gtggtgcggg tcagcgagac cacctcggaa tcggccacca
     4861 gcgtcccctt gctgaccgag gcaaggtagt tcagcttgaa ctcggtggtc gcagcccact
     4921 gccccggctg catcagcgga tagagcacca cgcccagcac atggtcgatg aaaccggcca
     4981 tgacgccgcc atgcatggtg ccgaaagcgg tcagcagttc atcgcgcacc tcgaccttgg
     5041 cccgcagcgt acccggccca aactcgacca gctccatgcc gatgaagccc ggcaggccac
     5101 ccatgttcct gttgttgctc tcgatcagcg agcgggcagt ggcttcgttg tagttcgaaa
     5161 actggggtaa ttccgccatg ttattgttct tccttaaaaa gtgattgaat ggcaccttgc
     5221 gccctgacag gaccccgctg aatctaccag cttggagtca aacatcaagg cggcaaccgg
     5281 gacaggcagt ggcgcagcca gcgccggtgc aaaccgccgc tggacttgaa aatttggaga
     5341 atcaacatga tgcgtgtcag cgaactctat ctgcacccgg tcaagtcctg cggcggccac
     5401 gccgtggagc gcgttgagct cgatccgcag gggccacgcg gcgaccgccg ctggatggtg
     5461 accgacccga ccgggcagtt tctcacccag cgcgagctgg cgcggatggc gctgatccag
     5521 gtcggagtga ccgacagcgg catcacgctc cgggcccccg gcttcgacac cctcaccgtc
     5581 atgacgccac cccaggaaca ggccagcgaa cgactggtca ccgtctggcg cgaccagtgc
     5641 cgagcactgc tggccgatgc ggccgccaat caatggctca gtgattttct gcaacggccg
     5701 gcacaactgg tccacctgcc cgacagcagc cggcggccgg tcgatccacg ctacgccacc
     5761 gccagcgatc aggtcagctt cgccgatggt tttccgctgc tgctgatcgg cgccgcctca
     5821 ctcgacgaac tcaacagccg tctcgatcag ccggtgtcga tgcgccgttt tcgccccaac
     5881 ctggtggtca gcggcggtgc cgcctttgcc gaggatggct ggcggcgcct ccgcatcggc
     5941 accatcacct tcagggtggc caagccctgc gcgcgctgcg cgattccggg catcgacccc
     6001 gacagcggcc agaccacggc cgaaccactg cgcacactgg cacggtttcg ccgccgcgat
     6061 ggggtgatct acttcggtca gaacctgctg catgacggac cgggcaccct gacagtgggc
     6121 gacccggtcg aactgctgga gtagcctgcc gcggcagaca cgccgtttcg gctatttggt
     6181 cgccgagcgc acccgcaccg ccagcacgtc gcacttggcg ccatgcagca cggcggtggc
     6241 cgtggaaccc agcaccaggc cgatgccatg gcgaccatgg ctgccgatga cgatcagatc
     6301 ggcctgctgt tcagtgacca gccgatggac ttccgccgcc ggccgtccat agatcagata
     6361 ctggcgctcg gccggaatgt tccactggct gcccagcccg cgcagccgcg ccttggcctg
     6421 ggtctcgatc tcctcctgga tggtgctgac atccatcgga atgtcgccac cataggcgaa
     6481 ggtgagaggt tcgatgacat ggatcaggct gagctcagcc tgatagcgcg ctgcgatcga
     6541 actggcctgt tcgacgacct gatcggcctc ggcggtgaga tcgatcgaca ccagaatttt
     6601 cttatagtcg gccatgcggc tgctcccaga gtgattgcct tgcaaaacca ggcggattct
     6661 cagtgatttt gtatcaattt gctaggctca cggccatgcc gcaaccccgg gtgacgctgt
     6721 gcgcctcgtg cttgaatccg gcaccgacac cgccatatcg agcccatgcc ccaacggtat
     6781 gaataaggta atatcggagc tctaccctgc aatgggtcaa tcggagaatc agatgccaga
     6841 tcgatcgcat ggggcaagcc atccaggcag cgtcgccggt gggaagcacg ccacatttcc
     6901 tcgctccacc ctcctccagc agcggaattt tcatctaacc tttagtggaa atgcaggggg
     6961 aagcagcccg gccggtggcc agcagggcga cacggccatc gaatccgcca agcccaagct
     7021 caagccgccc tcccggtatc aggtggtgat gctgaacgat gatttcacgc cgatggagtt
     7081 cgtggtcgat gtgttgcaga tgttctttgg catgccgctc gagaaggctt cccagatcat
     7141 gctgatcgtg cacacccgcg gtcgggcggt gtgcggtgtc tacagccgcg acatcgccga
     7201 aaccaaggcg tttcaggtca cccagtatgc ccgtgagcat caacatccct tgctgtgcga
     7261 aattgaacgt gtcgatgagt aattccgaat ccgaggtagc acatgttaag taaagatctc
     7321 gaagtctcgc tgaatctggc ctttcagggt gccagaaaca agcgacacga gttcatgacc
     7381 gtcgagcatc tgctgctggc actgctcgac aacgacattg cgatcgatgt gctgaaggcc
     7441 tgcggcatcg atctggagcg gctgcgcagc gatctcgaag agttcgtcga gagcaccacg
     7501 ccactgctgc ccgaaggcga caaggagcgc gacacccagc ccacgctcag ctttcagcgg
     7561 gtgctgcagc gggcgatctt ccatgtgcaa tcctccggca agcgcgaagt gaccggcgcc
     7621 aatgtgctgg tggcgatctt cagcgaacag gagagccacg cgatctttct gctgaaacag
     7681 cagcaggtgg cgcggatcga cgtggtcaac tacgtggccc atggcatcgc caaggtgtcg
     7741 cgccatcagg agcagctcga ccccggtccg gctgccggtg acgaggagcg cagcggcagt
     7801 gagggcagcg gccagcatcc gctggaggcc tttgccacca acctcaacca gcaggcgatg
     7861 cagggtcgca tcgatccact ggtcggccgt gaacatgagc tgcaacgggt ggtgcagatt
     7921 ctggcccggc ggcgcaagaa caacccgctg ctggtcggtg aggccggtgt cggcaagacc
     7981 gcgattgccg agggactggc caagcgcatc gtcgagggcg acgtgcccga ggtgatcgcc
     8041 aacagcgtgg tctactcgct tgatctgggc tcgctgctgg ccggcaccaa gtaccgcggc
     8101 gatttcgaaa agcggttcaa ggcgctgctg gccgaactga agaagcagga gaacgccatc
     8161 ctgttcgtcg atgagatcca caccatcatc ggcgccggcg cggcctcggg cggcgtgatg
     8221 gacgcatcga acctgctcaa gccgatcctg acctccggcg agctgcgctg catcggctcc
     8281 accaccttcc aggagtaccg tggcatcttc gacaaggacc gcgcactgtc acggcgcttc
     8341 cagaagatcg acgtgctcga accaagcgtc gacgagacgg tgcagatcct caaggggctg
     8401 aagtcccgct tcgaggccca ccacgggctg cgctacaccg ccggcgccct gcaggcggct
     8461 gccgagctgt cggcgcgcta catcaccgac cgccagctgc ccgacaaggc gatcgacgtg
     8521 atcgacgagg ccggcgccgc gcagcggctc aagccgccca gccgtcgcaa gaaggtgatc
     8581 ggcacccccg aggtcgagga ggtggtcgcc tcgatcgccc gcatcccgcc caagagcgtc
     8641 tcggcctccg acaaggaggt gctgcgcaac ctcgagggta acctcaagaa gctggtgttc
     8701 ggccaggatc aggcgatcga ggcgctggcc accgcgatca agctctcccg tgccggcctg
     8761 aagtcggtcg acaagccgat cggcgccttc ctgctctcgg gtccgaccgg cgtcggcaag
     8821 accgaggtga gtcggcaact ggccaacatc ctcggcgtgc agttgctgcg cttcgacatg
     8881 tccgaataca tggaacgcca caccgtctcg cggctgatcg gtgcaccgcc gggctatgtc
     8941 ggcttcgatc agggtggcct gctcaccgaa gcgatcacca agaacccgca ctgcgtgctg
     9001 ctgctcgacg agatcgaaaa ggcccatccg gaggtgttca acatcctgtt gcaggtgatg
     9061 gaccatggca ccctgaccga caacaacggc cggcgtgccg acttccgcaa cgtcatcctg
     9121 ctgatgacca ccaacgccgg cgccagcgag atgagccgca cctcgatcgg tttcaccaaa
     9181 caggaccaca gcagcgatgg catggaggcg atccgccgca tgttctcacc ggagttccgc
     9241 aaccgcctcg acgcgatcat cccgttcgcc ccgttgagcc cgcaggtgat cgtctcggtg
     9301 gtcgacaagt tcctggtcga gttgc
//
LOCUS       DAORCQ010000090         9307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5157_length_9307_cov_6.417748, whole genome shotgun sequence.
ACCESSION   DAORCQ010000090 DAORCQ010000000
VERSION     DAORCQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5157_length_9307_cov_6.417748"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..747
                     /locus_tag="PKI23_09225"
     CDS             46..747
                     /locus_tag="PKI23_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37158.1"
                     /translation="MPLTELILSNIDSLIAAIAGLGTAAYGLVDTSKAIRGGASNPGF
                     GHIETAVRPFLPDPGDSALGAPQILDTLRANWLNGVGKAEQKAAAKALIRLTLTQDNA
                     SKLARATHLAPAALQQLADSIANGTPLTPAQINLLGQFDTVLSALLDLGYERADQLYR
                     NSAKAFAAVTAMVLALVGGGLLSPDGTLAYLCSRQALLALLVGALSVPLAPIAKDLSS
                     ALSSAVSAIAASKRR"
     gene            754..1593
                     /locus_tag="PKI23_09230"
     CDS             754..1593
                     /locus_tag="PKI23_09230"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN37159.1"
                     /translation="MQNLYLNFRAAAIDGGIVEPYLLEGDGNQPQPALRALQWHELPP
                     LFAGKNVLFLSHGFNVNYAEGARALGRLDNRLALPEPDLFIGILWPGDAWLPVVNYPF
                     ESNDANHCGRLLADFCDRWLATAQSISFASHSLGARLILSAAQRMSRRLHLLCLTAAA
                     VDHDVLEAEFKRAAANAERIVVLASRDDQVLKLAFRVGDPLSDLLQGESITARPALGY
                     GGPSAGSAHLVERPWQIRSGYGHGDYLPPSGPQPSPTATAWQRVAEFIERAFYDQPRD
                     WPR"
     gene            complement(1637..3574)
                     /gene="acs"
                     /locus_tag="PKI23_09235"
     CDS             complement(1637..3574)
                     /gene="acs"
                     /locus_tag="PKI23_09235"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNN37160.1"
                     /translation="MSEIHLHQVPESAKARAHITRDDYLRMYRNSIEQPEPFWAEQAQ
                     KFLDWFSPWSTVSESDFRQGQAKWFIGATLNAAYNCIDRHLPQRADQVAILWEGDDPA
                     DSKKITYQELHQQVCRLANLLKARQVRKGDRVCIYMPMIPEAAYAMLACARIGAVHSV
                     VFGGFSPESLKDRILDSDCRLVITADEGLRGGRRVPLKQNCNQALERCPDVHSVIVVR
                     RTGGPIDWVDGRDIWYHEGIASVASDCPAEPMDAEDPLFILYTSGSTGKPKGVLHTTG
                     GYLLQAAMTHRYVFDYHDGDIYWCTADVGWVTGHSYIVYGPLANGATTLVFEGVPTYP
                     DASRFWQVIDKHQVNIFYTAPTALRALMGQGDQYVTRSGRQSLRTLGTVGEPINPEAW
                     EWYHRVVGEGRCPVVDTWWQTETGAIMISPLPGATDLKAGSATLPFFGVKPELLDADG
                     HIINGAGSGNLVIARSWPSQIRTVFGDHQRCVDTYFSTYPGYYFTGDGARRDEDGYYW
                     ITGRVDDVLNISGHRLGTAEIESALVLHPAVAEAAVVGYPHPIKGQGIYAYVTLMNEV
                     PPSDALRKELIDLVSKEIGAIAKPDIIQWAPGLPKTRSGKIMRRILRKVAANELDNLG
                     DTTTLAEPAVVDDLIKHRALQ"
     gene            complement(3746..4126)
                     /locus_tag="PKI23_09240"
     CDS             complement(3746..4126)
                     /locus_tag="PKI23_09240"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNN37161.1"
                     /translation="MESIMLKAKLHRACVTHAELEYEGSCAIDGQLLDLAGIREYEQI
                     QIYNVANGERFTTYAIRAEEGSRVISVNGAAAHKAKPGDRVIICAYAHYSAAQLASFK
                     PSLVYLNEDNTVSHASNSIPVQVA"
     gene            complement(4218..5069)
                     /gene="panC"
                     /locus_tag="PKI23_09245"
     CDS             complement(4218..5069)
                     /gene="panC"
                     /locus_tag="PKI23_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNN37162.1"
                     /translation="MELLHAIEPLRARLHARRRAGHRIGLVPTMGNLHQGHLDLVERA
                     RAQCDAVVVSIFVNPLQFGPQEDYDSYPRTLTADCALLEPLQVDWLFAPNVAEMYPQG
                     QGAGVIRVGTPAIADILCGANRPGHFSGVTTVVSKLFNIVQPDRAYFGEKDYQQLAII
                     RALVRDLDFPITITGVPTRREADGLAMSSRNGRLSSDDRQRAPLLYRALCETADRLRA
                     GDRNLDQLCAQARDTLTAAGFAVDYFTVLAADDLSPALPAQRNLVILVAAKIGSTRLI
                     DNLVVNV"
     gene            complement(5072..5866)
                     /gene="panB"
                     /locus_tag="PKI23_09250"
     CDS             complement(5072..5866)
                     /gene="panB"
                     /locus_tag="PKI23_09250"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNN37163.1"
                     /translation="MNAMTLNSLRALKRDGKRFSCLTAYDASFAHLLSSSGIEVLLVG
                     DSLGMVVRGHSSTVPVRMEEMVYHTHCVAAGNQGALLMADLPFMSYTTPAQALENSAR
                     LMQAGANVVKLEECQQLVEIIAALSGCGIPSCAHIGLAPQSVNKLGGYRVQGRDATAA
                     QAMIEHAVALEEAGADLLLLECVPSRLAETITRQLTIPVIGIGAGAATDAQVLVLHDM
                     LGITPGKPPKFVRNFMIGADSIQQAITGYRDAVLDGTFPGPEQSFS"
     gene            complement(5909..6439)
                     /gene="folK"
                     /locus_tag="PKI23_09255"
     CDS             complement(5909..6439)
                     /gene="folK"
                     /locus_tag="PKI23_09255"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNN37164.1"
                     /translation="MVRCHIALGSNLDEPLVQILTASRALAEIPDSTLVALSSCYRSA
                     PLDGMDQPWYVNRVAALDTQLSPEALLDALQRIERRQGRIRTVRWASRTLDLDLLLYG
                     DLVMESPRLRIPHPGMATRNFVLHPLAELVGDLILPTGQRLSELCRCCTDAGLIRIDL
                     TAVEPAIAGPALTHGR"
     gene            complement(6449..7816)
                     /gene="pcnB"
                     /locus_tag="PKI23_09260"
     CDS             complement(6449..7816)
                     /gene="pcnB"
                     /locus_tag="PKI23_09260"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNN37165.1"
                     /translation="MYTSRADFVATGLIAIEQTLAKPTVIPRDAHTLSRSQVSAAALK
                     VLYRLKDAGFAAYLVGGCVRDLLLGLTPKDFDIATDAMPEQVTQLFRNSRVIGRRFRI
                     VHVRFGQETIEVSTFRAHHSPFDEEDPDTEPLERRRSDSGMILRDNVYGRVDEDAGRR
                     DFTINALYYDIRDFTLHDYADGLRDLNARQIRMIGDPAIRYREDPVRMLRAVRFAAKL
                     DFTLAEETLKPIRTLRTLLLQVPAARLFDEVIKLFLTGHGLSSFQLLRAHGLFETLFP
                     ATQASIEAATPFAVALIEAAMHNTDLRLAEERPVNPAFIFAVLLWPVLTIRQQQLQHD
                     EGLPPIAALHQAAHEVLGEQVRITAIPRRFSTIVIEIWELQWRLPRRTPAAVAKLIAH
                     PRFRAGYDFLLLREQAGEALDGLGQWWSSYQIADEAERTALVATVTPPQSRRRSRVRR
                     RKSRD"
     gene            7883..8176
                     /locus_tag="PKI23_09265"
     CDS             7883..8176
                     /locus_tag="PKI23_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37166.1"
                     /translation="MPEAFHCKVCHSRAKLPRRVFVLQICRPAPDCYCYEPNLLVVIT
                     TMLHLDLPLRIGSSGGCAIIVVVPEASFLLFIVIVIVMSSSYTFGVPKLNSLK"
     gene            complement(8306..>9307)
                     /locus_tag="PKI23_09270"
     CDS             complement(8306..>9307)
                     /locus_tag="PKI23_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNN37167.1"
                     /translation="GLPGMVGRSPAMQAIYSHIHKVARTATTALIQGESGTGKELIAK
                     AIHALGPRRDQPLISINCAAIPESLIESELFGHEKGAFTGATASRTGLVEAADGGTLF
                     LDEIGELPLEAQARLLRVLQEGEIRRIGSVESKKVDVRLISATHRDLARMVKENRFRQ
                     DLYYRINVVKIELPPLRERGSDILEIAEHILDKVAQRLGRSALRFSPNAIQAITTYGW
                     PGNVRELENAIERAVVLSEQPVIDHPLLGIDVELIEVAELRRQQESKGTVPSKIAFEP
                     QEGLSLEDYFQQFVLQHQDQMSETELARKLGISRKCLWERRQRFSIPRSRTPAKRAG"
BASE COUNT         1629 a         2916 c         3069 g         1693 t
ORIGIN      
        1 gcacatctcc atcgatccat ccctgtaaac agcacggagc cgcccatgcc actgaccgag
       61 ctgatattga gcaacatcga cagcctcatc gccgccattg ccggcctggg caccgccgcc
      121 tacggattgg tcgacacctc caaggcgatt cgcggcggtg cctccaaccc ggggtttggc
      181 cacatcgaaa cagcggtgcg cccctttctg cccgaccccg gcgacagcgc cctgggtgcg
      241 ccgcagattc tcgacaccct gcgcgccaac tggctcaacg gggtcggcaa ggccgagcag
      301 aaggccgccg ccaaggcgct gatccggctg acgctgaccc aggacaacgc cagcaaactg
      361 gccagggcca cccaccttgc gccggctgcg ctgcaacagc tggccgacag catcgccaac
      421 ggcacgccac tgacgccagc gcagatcaac ctgctcgggc agttcgacac ggtgttgagc
      481 gcgctgctcg atctgggcta tgagcgggcc gaccagctct accgcaactc ggccaaggcc
      541 ttcgcggccg tcaccgcgat ggtgctggcc ctggtcggcg gcggactgct gtcgccggat
      601 ggcaccctgg cctacctctg ctccaggcag gcgctgctgg cactgctggt cggcgcgctg
      661 tcggtgccgc tggcgccgat cgccaaggat ctctcctcgg cactgtcgtc ggcggtctcg
      721 gcgattgccg cctcgaaacg ccgctgagcc gcgatgcaga acctctacct caactttcgc
      781 gccgcggcca tcgacggcgg catcgtcgag ccctacctgc tggagggcga tggcaaccag
      841 ccgcagccgg ccctgcgcgc gttgcagtgg catgagctgc cgccgctgtt cgccgggaag
      901 aacgtgctgt tcctcagcca tggcttcaat gtgaactatg ccgagggggc ccgcgcgctg
      961 ggccggctcg acaaccggct ggcgctgccg gaacccgatc tcttcatcgg catcctctgg
     1021 cccggcgatg cctggctgcc ggtggtcaac tacccgttcg agagcaacga tgccaaccac
     1081 tgcggccggc tgctggccga cttctgtgac cgctggctgg caacggcgca gagcatctcg
     1141 ttcgcttcgc acagtctcgg tgcccggctg atcctgagcg cggcacagcg catgtcacgg
     1201 cggctgcatc tgctctgtct gaccgcggcc gccgtcgacc atgacgtgct ggaggccgaa
     1261 ttcaagcggg ccgccgccaa tgccgagcgg atcgtcgtgc tcgcctccag ggatgatcag
     1321 gtgctgaagc tggcgttccg tgtcggcgat ccgctctccg acctgctgca gggcgagtcg
     1381 atcaccgccc ggccggcact cggctacggt ggcccgtcgg ccgggtcggc ccatctggtc
     1441 gagcggccct ggcagatcag aagtggctat ggccatggcg actacctgcc gccaagcggg
     1501 ccgcagccgt cacccacggc cacggcatgg cagcgggtgg cggagttcat cgaacgggcc
     1561 ttttacgacc agccgcgcga ctggccacgc tgaaactgcg tcagggaggc aagagcaccc
     1621 cctgacggca agcgcgctat tgcagcgcgc ggtgcttgat caggtcatcg acgacggccg
     1681 gctcggccag cgtcgtggtg tcgcccaggt tgtcgagctc attggccgcc accttgcgca
     1741 ggatgcgccg catgatcttg ccggagcggg tcttgggcag gccgggtgcc cactggatga
     1801 tgtccggctt ggcgatggca ccgatctcct tgctgaccag gtcgatcagc tccttgcgca
     1861 gcgcatcgct gggcggcacc tcgttcatca atgtgacata ggcatagatg ccctgtccct
     1921 tgatcgggtg gggatagccg accaccgccg cttcggccac cgccggatgc agcaccaggg
     1981 cactctcgat ctcggcggtg ccgaggcgat ggccggagat gttgagcacg tcatcgaccc
     2041 gaccggtgat ccagtagtag ccatcctcgt cgcgacgggc gccatcgccg gtgaagtaat
     2101 agccgggata ggtcgagaaa taggtgtcga cacagcgctg gtggtcaccg aagacggtgc
     2161 ggatctggct gggccaactg cgggcgatca ccaggttgcc gctgccggcg ccgttgatga
     2221 tgtggccatc ggcatcgagc agctccggct tgacgccgaa gaacggcagc gtggcggagc
     2281 cggccttgag gtcggtggca ccgggcagcg gcgagatcat gatcgcgccg gtttcggtct
     2341 gccaccaggt gtcgaccacc gggcagcgcc cttcgccgac cacccggtgg taccactccc
     2401 aggcttcggg attgatcggt tcgccgaccg tgccgagcgt gcgcagcgat tgacgcccgc
     2461 tgcgggtcac atactggtcg ccctgcccca tcaaggcccg cagggccgtg ggtgcggtgt
     2521 agaagatgtt gacctgatgc ttgtcgatca cctgccagaa gcgcgaggca tcgggatagg
     2581 tgggcacccc ctcgaagacc agagtggtgg cgccattggc gagcgggcca tagacgatgt
     2641 aggagtggcc ggtgacccag cccacatcgg ccgtgcacca gtagatgtcg ccatcgtggt
     2701 agtcgaagac atacctgtgg gtcatggcgg cctgcaacag ataaccgccg gtggtgtgca
     2761 gcaccccctt gggtttgcca gtcgaaccgg aggtgtagag gatgaagagc ggatcctcgg
     2821 catccatcgg ctcggccgga caatcgctgg cgacgctggc aatgccctcg tggtaccaga
     2881 tgtcacgtcc atcgacccag tcgatcggac cgccggtgcg gcgcacgacg atgaccgaat
     2941 gaacatcggg gcagcgctcc agcgcctggt tgcagttctg cttgagcggc acccgccggc
     3001 cgccgcgcag cccctcgtcg gcggtgatca ccagccggca gtcggagtcg agaatccggt
     3061 ccttcagtga ctccggcgag aagccgccaa agacgacgga gtggaccgca ccgatccggg
     3121 cgcaggccag catggcatag gcggcctcgg gaatcatcgg catgtagatg cagacccggt
     3181 cccccttcct gacctggcgc gccttcagca ggttggcgag ccggcacacc tgctggtgca
     3241 gctcctggta ggtgatcttt ttcgagtcgg ccggatcatc cccctcccag agaatggcga
     3301 cctgatcagc ccgctgcggc agatggcggt cgatgcagtt gtaggcggca ttcagggtcg
     3361 cgccaatgaa ccatttggcc tgtccctgac gaaagtccga ttcgctcacg gtgctccagg
     3421 ggctgaacca gtcgaggaac ttctgcgcct gttcggccca gaacggctcg ggctgttcga
     3481 tcgagttgcg gtacatccgc agatagtcgt cgcgggtgat atgcgcgcgc gccttggcgc
     3541 tctctggaac ctggtggaga tggatttcgg acactgtgac tccctgcatc ggcgtggctt
     3601 atggtgatgg catgccaagt cgacgctggc cctcttgcag agaggcagga cgtgacttgt
     3661 ggatgtgccg agagtgtact cgtcgcctgt ggagtcgggc aaccgcccgg cgcgatgcgc
     3721 cgggcggtac agaaagagcg gagggtcagg ccacctggac cgggatggag ttgctggcgt
     3781 ggctgacggt gttgtcttcg ttcagataga ccagcgaggg cttgaagctg gccagctggg
     3841 ctgcgctgta gtgggcatag gcacagatga tcacccggtc acccggcttg gccttgtgcg
     3901 cggccgcgcc attgaccgag atcacccgcg aaccctcttc ggcccggatg gcataggtgg
     3961 tgaaacgctc gccattggcg acgttgtaga tctgaatctg ctcatattca cggatgccag
     4021 ccagatccag aagctggcca tcgatcgcgc acgaaccttc atattcaagc tcggcatggg
     4081 tgacacatgc ccgatgtaac ttggccttga gcatgatgga ttccatacag ccacacctct
     4141 tcagctggag ttatagcctc tttccggatt cctgccggaa agcgtgggcg gcagtatgcc
     4201 gcaaagaatt accccgttca aacatttacc accagattgt caatcagccg ggtcgagccg
     4261 attttggccg ccaccagaat gaccagattc cgctgcgctg gcagtgccgg cgacagatca
     4321 tcggcggcca gaacggtgaa ataatcgacg gcaaaaccag ctgccgtcag agtgtcacgg
     4381 gcctgtgcgc agagctgatc gaggttccgg tcaccggcgc gcaggcggtc ggccgtctcg
     4441 cacagcgccc ggtaaagcag tggcgcccgt tgccgatcgt cgctgctgag gcggccattg
     4501 cgcgaactca tcgccaggcc atcggcttcg cggcgggtcg gcacgccggt gatggtgatc
     4561 ggaaagtcga ggtcacgcac cagcgcacga atgatggcca gttgctggta atccttctcg
     4621 ccgaaatagg cccggtcggg ctgaacgatg ttgaacagct tgctgaccac ggtggtcacg
     4681 ccgctgaagt ggccgggacg gttggcgcca cagaggatgt cggcgatcgc cggggtgccg
     4741 acgcggatca ctccagcccc ctgaccctgc gggtacatct cggcgacgtt gggggcaaac
     4801 agccaatcga cctgcaatgg ctccagcagt gcgcaatcgg cggtcagcgt gcgcggatag
     4861 ctgtcgtaat cctcctgcgg tccgaactgc aacgggttga cgaagatgct gacgaccacg
     4921 gcgtcacact gcgcgcgcgc ccgttcgacc agatcgagat ggccctgatg cagattgccc
     4981 atggtcggca ccaggccgat gcggtggccg gcacgccgcc gtgcatgcag ccgtgcgcgc
     5041 agtggttcga tggcgtgcag cagctccatc gtcaggagaa gctctgctcc gggccaggga
     5101 aggtgccgtc gagcacggca tcgcgataac cggtgatggc ctgctggatg ctgtcggccc
     5161 cgatcatgaa attcctgacg aactttggtg gcttgccggg ggtgatgccg agcatgtcat
     5221 gcagcaccag cacctgggca tcggtggcgg caccggcacc aatgccgatg accggaatgg
     5281 tcagttgccg ggtgatggtc tcggccagcc ggctgggcac gcattcgagc agcagcaggt
     5341 cggcaccggc ctcttcgagt gccacggcat gctcgatcat cgcctgcgcg gccgtggcat
     5401 cgcgtccctg cacccggtag ccgcccaatt tgttgacgct ctggggggcc agaccgatgt
     5461 gggcgcaact gggaatgccg cagccactga gtgcggcgat gatctcgacc agctgctggc
     5521 actcctccag cttgacgacg ttggcgccgg cctgcatcag ccgggccgaa ttttccagtg
     5581 cctgggctgg ggtggtgtaa ctcatgaagg gcaggtcggc catcagcagc gcaccctggt
     5641 tgccggcggc aacgcaatgg gtgtggtaga ccatctcttc catccgcacc ggcacggtcg
     5701 acgaatggcc acgcaccacc atgccgaggg agtcgccgac cagcagcact tcgatgccgc
     5761 tgctgctgag caggtgggca aagctggcgt cataggcggt gaggcaggaa aaccgcttgc
     5821 cgtcgcgctt gagcgcccgc aggctgttca gggtcatggc attcacgcgg tggctccttc
     5881 tgtctgactg ggcatgcggt tcgcagggtc agcggccgtg ggtgagcgcg ggcccggcga
     5941 tcgccggttc cacggcggtg agatcgatgc ggatcaggcc agcatcggta cagcaccgac
     6001 acagctcgct cagccgctgc ccggtcggca gtataaggtc gcccaccagt tccgccaaag
     6061 ggtgcagcac gaagttccgc gtcgccattc ccggatgggg aatccgcagc cgggggctct
     6121 ccatcaccag gtcgccatag agcagcaggt cgagatcgag cgtgcgggac gcccagcgca
     6181 cggtgcggat gcggccctgt cgccgttcga tccgttgcag tgcatcgagc agcgcttccg
     6241 gtgacagctg ggtgtcgagc gcggcgaccc gattgacgta ccagggctga tccatcccat
     6301 ccagcggtgc gctgcggtaa caactggaga gcgcaaccag cgtgctgtcg ggaatttccg
     6361 ccaacgcccg ggaggcggtg agaatctgca ccagcggctc atccaggtta ctgcccagcg
     6421 cgatatggca acgcacgatg cagatggctc agtcgcggga tttgcgccgc cgcacccggc
     6481 tgcggcgcct ggattggggg ggcgtaacag tggcgaccaa tgccgtccgt tcagcctcgt
     6541 cggcaatctg atagctgctc caccactgac cgagaccatc gagcgcttca cccgcctgtt
     6601 cgcgcagcag cagaaagtcg tagccggcac gaaagcgtgg atgcgcgatc agcttggcca
     6661 ccgccgctgg cgtgcgtcgg ggcagccgcc actgcagctc ccagatctcg atgacgatgg
     6721 tggagaagcg gcgcggaatg gccgtgattc gcacctgctc gcccagcacc tcgtgggcgg
     6781 cctgatggag tgccgcgatc ggcggcagcc cctcgtcatg ctggagctgc tgctgtcgga
     6841 tcgtgaggac cggccacaac agcaccgcga agatgaaggc cggattgacc ggacgttcct
     6901 cggccaggcg caggtcggtg ttgtgcatcg ccgcttcgat gagcgcaacg gcaaagggtg
     6961 tcgcggcctc gatgctggcc tgggtggctg gaaagagcgt ctcgaacagg ccgtgcgccc
     7021 gcagcagttg aaacgagctc aatccatggc cagtcaggaa cagcttgatc acctcatcga
     7081 acaggcgtgc cgcaggcacc tgcagcagca gggtgcgcag cgtcctgatc ggtttgaggg
     7141 tctcctcggc cagcgtgaag tcgagtttgg cggcaaagcg caccgcgcgc agcatgcgca
     7201 ccggatcctc gcggtagcgg atggccgggt caccgatcat cctgatctgc ctggcgttca
     7261 ggtcgcgcaa tccatcggcg tagtcgtgca gcgtgaagtc gcggatgtcg tagtagagcg
     7321 cgttgatggt gaagtcacgc cgtccggcat cctcgtcgac ccggccatag acattgtcgc
     7381 gcaggatcat gccgctgtcg gaacggcgcc gctcgagcgg ttcagtgtcg ggatcttctt
     7441 cgtcgaaggg cgagtgatgg gcgcgaaagg tcgagacctc gatggtctcc tggccgaaac
     7501 ggacatgcac gatgcgaaaa cggcgaccga tcacccggga attgcgaaag agctgagtga
     7561 cctgctccgg catcgcatcg gtggcaatgt cgaaatcctt gggtgtcagg cccaacagca
     7621 gatcgcgcac gcagccaccc accagatagg ccgcgaaacc ggcatccttc agccgataga
     7681 gcaccttgag cgcggcggcg ctgacctgac tgcgggagag ggtgtgggcg tcacggggga
     7741 tgacggtcgg cttggcaagc gtctgttcga tggcaatgag acctgtggca acaaaatccg
     7801 cgcgcgaagt atacaccacg ccacatcgat accgcgtcga gccgaaagaa gggaagtgga
     7861 tttcagatga gtttgcagtg gaatgcctga ggcattccac tgcaaggtgt gtcactcaag
     7921 agccaagctg ccgcgccgtg tctttgtttt acagatttgc cggccagcgc cggattgtta
     7981 ttgttatgag ccaaacctgc ttgttgttat cacaacgatg ctgcacctcg atctgcctct
     8041 tcggatcggc agctcaggtg gctgcgcaat tattgttgtt gtacccgaag cgtcgttttt
     8101 attgtttatt gttattgtta ttgttatgtc ctcttcctat acatttggcg tgccaaaatt
     8161 aaattcactt aaataacaac aagttgaaat attatcgtgg tttgctaaac ggcggggaaa
     8221 cagttttctg ttaccgtccc cctcgttctg gggaccacgc cgcgcgacag gtaacatcgc
     8281 ggcacggcgt tgcggtgaca ggcggctagc cagccctctt ggcgggtgtg cgactgcgcg
     8341 ggatgctgaa gcgctgccgg cgctcccaca gacatttacg gctgatgccg agcttgcgcg
     8401 ccagctcggt ctcgctcatc tggtcctgat gctgcagcac gaactgctgg aagtaatcct
     8461 cgagcgacag cccctcctgc ggttcaaagg cgatctttga cggcaccgtg cccttgcttt
     8521 cctgctgtct gcgcagttcg gccacctcga tcaactcgac atcgatgccg agcagcgggt
     8581 ggtcgatgac cggctgctcg ctgagcacca ccgcccgttc gatggcgttt tcgagctcgc
     8641 ggacattgcc gggccaaccg taggtggtga tggcctggat ggcgttgggc gagaaccgca
     8701 gcgcggagcg ccccagacgc tgggcaacct tgtcgagaat gtgttcggcg atctcgagga
     8761 tgtcgctgcc gcgctcgcgc agcggcggca gttcgatctt caccacattg atgcggtagt
     8821 agagatcctg gcgaaaccgg ttctccttga ccatgcgcgc cagatcgcgg tgggtggccg
     8881 agatcagacg cacatcgacc ttcttcgatt cgaccgaacc gatgcggcga atctcgccct
     8941 cctgcagcac ccgcagcagg cgggcctggg cctccagcgg cagttcgccg atctcatcca
     9001 gaaagagggt gccgccatcg gcggcttcga ccaggccggt gcggctggcg gtcgcgccgg
     9061 tgaaggcgcc cttttcgtgg ccaaagagtt cggattcgat caggctttcg ggaatggccg
     9121 cgcagttgat gctgatcagc ggttggtcac ggcgtggccc cagcgcatgg atggccttgg
     9181 cgatcagctc cttgccggtg cccgattcac cctggatcag cgcggtggtg gcggtcctgg
     9241 ccaccttgtg gatgtggctg tagatcgcct gcatcgccgg tgagcgcccg accatgccgg
     9301 gcaggcc
//
LOCUS       DAORCQ010000091         9181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5258_length_9181_cov_5.815582, whole genome shotgun sequence.
ACCESSION   DAORCQ010000091 DAORCQ010000000
VERSION     DAORCQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_9181_cov_5.815582"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKI23_09275"
     CDS             <1..612
                     /locus_tag="PKI23_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNN37168.1"
                     /translation="PTATGHCHLLDLGANVALTSEHLHQFAIMGSVAAAAMDGLEAPR
                     VGLLNIGEEEIKGNEQIKLASQLIRENARLNYIGYIEGDDIFRGAADVVVCDGFVGNV
                     AIKTSEGLAELIDQRLNAAFRANILSRFVGLLAWPILRRLRRQIDPGRYNGATLLGLQ
                     GILVKSHGDADVDGFRQAIEVAVREVDSDVPQQIHRMLEATLA"
     gene            658..1611
                     /gene="fabD"
                     /locus_tag="PKI23_09280"
     CDS             658..1611
                     /gene="fabD"
                     /locus_tag="PKI23_09280"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNN37169.1"
                     /translation="MSGFTAFVFPGQGSQQVGMLADLAQHFALVQETFAEASEQLGFD
                     LWQLTQQGPEEQLNQTANTQPALLAASVAIWRVWRSLAAPLPEWVAGHSLGEYSALVA
                     AESIDFADAVRLVRTRGLLMQEAVPVGVGAMAAILGLEDAEVEAACAEAAGDEVVAAA
                     NYNAPGQVVIAGHRTAVERAIELMKARGAKRAMPLPVSVPSHCALMRPAAERLRGELA
                     SIQIRAPQIKVVQNVHAREEHQIEAIRANLVAQLDHPVRWTRSVETLAAAGVATTIEC
                     GPGKVLSGLNKRIDKRLNTFAIQESASLHEVATQLANGSIG"
     gene            1650..2393
                     /gene="fabG"
                     /locus_tag="PKI23_09285"
     CDS             1650..2393
                     /gene="fabG"
                     /locus_tag="PKI23_09285"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNN37170.1"
                     /translation="MSQEARIALVTGASRGIGQAIALALGAQGATVVGTATTEAGAQK
                     ISATLAEQGISGSGFCLDVCSDESVAAVLQQIEAQFGTPLILVNNAGITRDNLLMRMK
                     SNEWDEVIDTNLSALYRTSKAMLRGMTKARWGRIVNIGSVVGAMGNAGQTNYAAAKAG
                     MGGFTRALAREVGSRGITVNAVAPGFIDTDMTRALDEAHRQTLQQQIPLGRLGSAAEV
                     AAVVAFLCSESAGYVTGETLHVNGGMYMA"
     gene            2531..2770
                     /gene="acpP"
                     /locus_tag="PKI23_09290"
     CDS             2531..2770
                     /gene="acpP"
                     /locus_tag="PKI23_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNN37171.1"
                     /translation="MSSIEDRVKKIICEQLGVKEEDITNASSFVEDLGADSLDTVELV
                     MALEDEFETEIPDEDAENITTVQQAIDYVKRNLEA"
     gene            2866..4107
                     /gene="fabF"
                     /locus_tag="PKI23_09295"
     CDS             2866..4107
                     /gene="fabF"
                     /locus_tag="PKI23_09295"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNN37172.1"
                     /translation="MSRRRVVVTGMGMLTPLGNTVAETWAAILAGRSGIGPIETFDVT
                     AYATQFGGGLKGFDVEQYIPQRDARRMDPFIQYGMAAGIQAFEDAAITVTEANAERIG
                     VAIGSGIGGLLTIEQSRDVVRDKGPKRASPFFVPSAIINMISGNLSIRYGLKGPNIAI
                     ATACTTGAHNIGISARLIQSGDADVMVCGGAEMATTEGGLAGFSSAKALSTRNEAPQA
                     ASRPWDRDRDGFVLSDGAGALVLEEYEHARARGARILAELAGFGMSADAHHITSPPED
                     GAGAAQAMRSALLDARLTAEQIQYINAHGTSTPLGDVAESKAIESLFGEASRTVAVSS
                     TKSMIGHLLGAAGAVEAIFCILALRDQVAPPTINLDNPDPECRLDYVPHQARPMRIDA
                     ALSNSFGFGGTNGSLVFSRMR"
     gene            4235..5137
                     /gene="pabC"
                     /locus_tag="PKI23_09300"
     CDS             4235..5137
                     /gene="pabC"
                     /locus_tag="PKI23_09300"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNN37173.1"
                     /translation="MSTLRDAAIPGSDLRLLWVNGRAEPFLHPLDRGLAYGDGLFQTM
                     RVVGGAIPLLGHHLQRLSEGLQRLAIECDLASIEREIGQFLAALGSEEAKADWVLKLT
                     LTRGVAGRGYLPAPGVVPTRMLALFPGTPFPAPQSESGVTVRLCRLRLSRQPALAGIK
                     HLNRLEQVLARSEWHDARIAEGILRDEAGQLIDGVISNLFAVRGGVVETPRLDQCGVA
                     GAMRAFIIARCAALSIPCREVALSLEQVLSAEEIFLSNSLFGLWPVAQWLDEAGGDLG
                     WRPGGNPVLRRLQVEVNSLFLAAS"
     gene            5134..6186
                     /gene="mltG"
                     /locus_tag="PKI23_09305"
     CDS             5134..6186
                     /gene="mltG"
                     /locus_tag="PKI23_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNN37174.1"
                     /translation="MKRSLRTLTLWMALFGVVTGAAALFVHSSVQAWLNRPLPAQGQL
                     FEVLPGDSLWAVGQRLQRQGLLSPAWLLTAVGQWHGVAGRIQAGEYQLDVGSTPQSLL
                     QKLLRGEVVVYRVTFVEGWTLRQVLDLLQRQPKLEHRLAAADDAELARVLELDSAQPE
                     GWIYPDTYHYRKGESDVQLLRRAHQSMKKRLAEQWSGRATDLPLKSPYEALILASIVE
                     KETGQGSERPQIAGVFVRRLHSDMPLQTDPTLIYGLGACFDGNLTLADLRRPGPYNSY
                     LNKGLPPTPIGLPGEAAIQAVLHPAAGDALYFVAKGDGSHHFSATLEAHQQAVEQYQQ
                     RQRRADYRSAPRNGGQ"
     gene            6188..6829
                     /gene="tmk"
                     /locus_tag="PKI23_09310"
     CDS             6188..6829
                     /gene="tmk"
                     /locus_tag="PKI23_09310"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009877617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNN37175.1"
                     /translation="MQRGRFITIEGSEGAGKSSCLRKISALLQQLSIEHRITREPGGT
                     ELAEAIRDLLLRPWAEPVQPLTELLLLFAGRAQHIQQLIEPALAAGIWVVSDRFTDAT
                     YAYQGGGRAVPQSMIAQLEQLVQGELRPDLTLFLDLPVADGLRRIDGRDGELDRFERE
                     QIDFFERIRACYLARSAAEPERIRRIDASAPLDQVTAAIEQTLRQAIQRWSAP"
     gene            6826..7854
                     /gene="holB"
                     /locus_tag="PKI23_09315"
     CDS             6826..7854
                     /gene="holB"
                     /locus_tag="PKI23_09315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNN37176.1"
                     /translation="MSVPREAGQDPLAPPLPWQRPQWQRLVELQQQQKLAHALLLSGP
                     QGIGKRCFAERLATWLLCHQPGREQACGACRSCGWIRAGTHPDLFWLLPESPARVIKI
                     DQVREANEFMQLSAQAGHGKVVIIDPADQLNRNAANALLKSLEEPAAERWLLLVSSRY
                     GMLLPTIVSRCQRLTMVEPTAPQAQAWLRDHTDAELPWDEILAASRNRPLRALDLARE
                     PLWKQYNRALTLLSAISTGRSDPLLLAEGGEQFDPELLLDWWWGWLVAAIRLASGLAA
                     DLPLPTELTTRAGQASPLALFDFLNRLEQARTIQGQQITLNRSLLLGDLALRWRDAFA
                     RRQPLQMG"
     gene            7924..8274
                     /locus_tag="PKI23_09320"
     CDS             7924..8274
                     /locus_tag="PKI23_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNN37177.1"
                     /translation="MKPRQGSGHGILSLTIKDKAVLFAAYMPFVRHGGLFIPTSRNYQ
                     LHDEVFMLLQLMEEPDKLPVAGKVVWVTPKGAQSNRVAGIGVQFSGQDDTARNKIETY
                     LAGMLETDRPNHTL"
     gene            8316..9119
                     /locus_tag="PKI23_09325"
     CDS             8316..9119
                     /locus_tag="PKI23_09325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNN37178.1"
                     /translation="MFVDSHCHLDRIDLTPHGGSLEAAIAAARAAGVSGILNVAVDLN
                     EFPQVLATAASQPAVWATCGLHPLEVAEPLPTVEQLLALAADARVVALGETGLDGHYG
                     SEQSALQQESFRRHLLASRALRKPVVVHSRDARAQTLALLEAHADPAVGGVLHCFTED
                     WAMAERALELNFHVSFSGIVTFRNADALREVARRVPLDRLLIETDAPYLAPIPYRGKR
                     NEPRYLPAVGHFVAQLRGIAPQALAEQTSANFCTLFRIDPALLAVATRS"
BASE COUNT         1589 a         2861 c         3099 g         1632 t
ORIGIN      
        1 ccgaccgcca ctggccattg ccatctgctt gatctcggcg ccaatgtcgc gctcacctcc
       61 gagcatctgc atcagtttgc catcatgggc tcggtggccg ctgccgcgat ggatggtctt
      121 gaggcgccgc gggtgggcct gctcaacatc ggtgaggagg agatcaaggg caatgagcag
      181 atcaagctgg catcgcaact gatccgggag aatgcccggc tcaactacat cggctacatc
      241 gaaggcgatg acatctttcg cggcgccgcc gacgtggtgg tgtgtgacgg ttttgtcggc
      301 aatgtcgcca tcaaaaccag cgaagggctg gccgaactga tcgaccagcg gctcaatgcg
      361 gcgttcagag ccaacattct gagccggttc gttggcttgc tggcatggcc gatcctgcgg
      421 cggctgcgca gacaaatcga tcctggccgc tataatggcg ccaccctgct ggggctgcag
      481 ggcattctgg tcaagagcca tggcgatgcc gatgtcgatg gcttccgtca ggccatcgag
      541 gtggcggtac gggaggtcga ctccgatgtg ccgcaacaga ttcaccgcat gctcgaagcc
      601 acactggcgt aatcaagaat aatcacactc tgccgtcgat ccacgatgag ggatgcaatg
      661 tctggcttca ccgctttcgt ctttccgggc caaggttcac agcaggtcgg catgctggcc
      721 gatctggcgc agcacttcgc gctggtgcag gagacctttg ccgaggccag cgaacagctt
      781 ggcttcgatc tgtggcagtt gacgcagcag gggccggaag agcagctcaa ccagaccgcc
      841 aacacccagc cggcgctgct ggcggccagt gtggcgatct ggcgggtgtg gcgctccttg
      901 gccgcaccgc tgccggagtg ggtggcagga cacagcctcg gtgaatactc cgcgctggtg
      961 gcggccgagt cgatcgactt tgccgatgcg gttcggctgg tgcgcacccg tgggctgctg
     1021 atgcaggagg cggtgccggt cggcgtcggt gcgatggccg ccattctggg tctggaagat
     1081 gccgaggtcg aggcggcctg cgctgaagcg gctggtgatg aagtggtggc cgcagccaac
     1141 tacaacgctc ccgggcaggt ggtgattgct ggccaccgga cggcggtcga gcgggccatc
     1201 gagttgatga aggcgcgcgg cgccaagcgt gccatgccgt tgccggtcag tgtcccttcg
     1261 cactgcgcgc tgatgcgccc ggcggcggag cggctgcgcg gcgagctggc gtcgattcag
     1321 attcgcgcgc cgcagatcaa ggtggtgcag aacgttcatg cccgtgagga gcaccagatc
     1381 gaagcgattc gcgccaacct ggtcgcccag ctcgaccacc cggtgcgctg gacccgctcg
     1441 gtcgagacgc tggctgctgc cggcgtggca accaccatcg aatgtggacc gggcaaggtg
     1501 ctgtccgggc tcaacaagcg catcgacaag cgcctgaaca ccttcgccat ccaggagtcg
     1561 gcctcactgc acgaggtggc gacgcagctc gccaatggca gcatcggctg atcgcatcag
     1621 ttttttcatc cccatcagga gagcagagcg tgagtcagga agccagaatt gcactggtca
     1681 ccggcgccag ccggggcatc ggccaggcca tcgcgctggc gctgggtgcc cagggtgcca
     1741 ccgtggtcgg caccgcaacg accgaggccg gcgcgcagaa gatttcggcc accctggccg
     1801 aacagggcat cagcggcagc ggcttctgtc tcgatgtctg cagcgatgaa tcagtcgctg
     1861 cggtgttgca gcagatcgaa gcacaattcg gcacaccgct gattctggtc aacaacgccg
     1921 gcatcacccg cgacaacctg ctgatgagaa tgaagtcgaa cgagtgggat gaggtgatcg
     1981 acaccaatct gagtgccctc taccgcacct ccaaggccat gctgcgcggc atgaccaagg
     2041 cgcgctgggg acgcatcgtc aacatcggtt cggtggtagg ggccatgggc aatgccggcc
     2101 agaccaacta tgctgcggcc aaagcgggca tgggtggctt cacccgggcg ctggcccgtg
     2161 aagtgggctc gcgcggcatc accgtcaatg cggtcgcgcc gggcttcatc gacaccgaca
     2221 tgacccgcgc gctggatgag gctcatcgtc agacactcca gcagcagatt ccgctcggac
     2281 gccttggatc ggcggccgaa gtggcggcgg tggtggcatt tctttgcagt gaatcagccg
     2341 gttacgtcac cggagagacc ctgcatgtca atggcggaat gtatatggct taaggtcaac
     2401 tgtttgggct acaatgccgc cgcaaaatcg gcgggcccaa ttcgggttgt tgccttttca
     2461 tgaagatgcc tgaagggtgg ccgttggccc acttcttgga attcaacatt atcgggagat
     2521 aatacgcacc atgagtagca tcgaagaccg cgtcaagaag atcatttgcg aacagcttgg
     2581 cgtgaaggaa gaagacatca ccaacgcctc ttcctttgtc gaggatctgg gcgcagactc
     2641 cctggacacc gtcgaactgg tgatggcgct cgaagatgaa ttcgagaccg aaattcccga
     2701 tgaagatgcc gaaaacatca cgaccgttca gcaagccatc gactacgtca agcgcaatct
     2761 cgaagcctga gaccgcgcct gttcagtcaa gtgagcagtg gtaaagccgt ctctgtcccg
     2821 cacagagcgg cttttgtttt gggcgagtga aagagggtga atgccgtgtc aagacgcagg
     2881 gtggtggtga cgggcatggg gatgctgacc cccctcggca acacggttgc cgaaacctgg
     2941 gctgcgatcc tggctggacg cagcggcatc ggcccgatcg agaccttcga tgtgaccgcc
     3001 tacgccacgc agtttggtgg aggattgaaa gggttcgacg tcgagcagta catcccgcag
     3061 cgggatgccc gccgcatgga tcccttcatt caatatggca tggcggcagg cattcaggcc
     3121 ttcgaggatg cggcgatcac ggtcaccgaa gccaacgccg agcgcatcgg cgtggccatc
     3181 ggctcgggca tcggtggcct gctgaccatc gagcagagcc gcgacgtggt gcgggacaaa
     3241 ggcccgaagc gcgcctcacc tttctttgtg ccgtcggcca tcatcaacat gatctcgggc
     3301 aacctgtcga tcaggtatgg tctgaagggg cccaacatcg ccattgccac cgcctgcacc
     3361 accggggcgc acaacatcgg catttcggcg cggctgatcc agtcgggcga tgccgacgtg
     3421 atggtctgcg gcggtgccga gatggccacc accgaaggtg gtctggccgg tttctcctcc
     3481 gccaaggcgt tgtcgacccg caatgaggcg ccacaggccg ccagtcggcc ttgggaccgt
     3541 gaccgggatg gtttcgtgct gagcgatggt gccggtgcac tggtgctgga ggagtatgag
     3601 cacgcccggg cccgcggtgc acggattctg gccgaactgg cgggcttcgg catgagcgcc
     3661 gatgcgcacc acatcacctc accgccggaa gatggcgccg gcgccgcgca ggcaatgcgc
     3721 agtgcgctgc tggatgccag actgactgcg gagcagattc agtacatcaa tgcccatggc
     3781 acctcgaccc cgctgggtga tgtggccgaa tcgaaggcca tcgagtcgct gttcggcgag
     3841 gccagccgca ctgtcgcggt cagctccacc aagtcgatga tcggccatct gctcggcgcg
     3901 gccggtgcgg tcgaggcgat cttctgcatc ctggcactgc gcgatcaggt ggcgccgccc
     3961 accatcaacc tcgacaaccc cgatcccgag tgccgcctcg actatgtgcc gcaccaggct
     4021 cgaccgatgc gcatcgatgc cgcgctctcc aactcctttg gctttggcgg caccaacggc
     4081 agcctggttt tttccagaat gcgctgattg cccaggcggc ccggcagccg tgaggccttg
     4141 ccgccgctgc atcggcgttg cgtcaccacg ccgggtgcga caggtggcgt cgctgttgat
     4201 tccttccaga acacgtccac tgacacgatg accgatgtcg acactccgtg acgccgcaat
     4261 accaggcagt gatcttcggc tgctgtgggt caatggccgg gccgagccgt ttctgcatcc
     4321 gctcgaccgt ggtctggcct atggcgacgg gctgttccag accatgcggg tggtgggggg
     4381 cgccatcccg ctgctgggtc accacctgca acggctgagc gaggggctgc agcggctggc
     4441 gatcgagtgt gaccttgcat cgatcgagcg ggagatcggc cagtttctgg ccgcgctggg
     4501 atcggaggag gccaaggccg actgggtcct gaagctgacc ctgacccgcg gggttgccgg
     4561 acgcggctat ctgccggcgc ccggagtggt gccgacgcgg atgctggcgc tgtttcccgg
     4621 aacgcccttt ccggcaccgc agagcgagtc gggggtgacg gttcggctct gccggctgcg
     4681 gctctcccgt cagccggcat tggcgggcat caagcatctg aaccgcctcg aacaggtgct
     4741 ggcgcgcagc gagtggcatg atgcgcggat cgccgagggg atcctgcgcg acgaggcagg
     4801 ccagttgatc gatggcgtga tcagcaacct cttcgccgtg cgcggcggtg tggtggagac
     4861 gccgcggctc gaccagtgcg gagtggccgg ggcgatgcgc gccttcatca tcgcgcgctg
     4921 tgctgcgctg tcgattccct gtcgcgaggt ggcactgtcg ctcgaacagg tgttgtcggc
     4981 cgaggagatc tttctcagca acagcctgtt tggtctctgg ccggtggcgc agtggctcga
     5041 tgaggcgggc ggtgacctgg gctggcgccc tggcgggaat ccagtgctgc ggcgcctgca
     5101 ggtggaagtg aattcactct ttctcgctgc gtcatgaagc gatcgcttcg cacgctcacg
     5161 ctgtggatgg cgctgtttgg cgtggtgacg ggtgccgccg cgctctttgt ccattcgagc
     5221 gttcaggcat ggctgaaccg gccacttccg gcgcaggggc agttgttcga ggtccttccc
     5281 ggcgattcgc tctgggcggt ggggcagcgg ctgcaacggc agggtctgct gtcacccgcc
     5341 tggctgctga ctgccgtcgg tcagtggcac ggggtggcgg ggcggattca ggcgggcgaa
     5401 tatcagctcg atgtcggcag cacgccacaa tcactgctgc agaagctgct gcggggcgag
     5461 gtggtggtct atcgcgtcac cttcgtcgag ggctggacgc tccggcaggt gcttgacctg
     5521 ctgcagagac agcccaaact ggagcaccgt ctggccgcag cggatgatgc cgaactggcc
     5581 agggtgctcg agctcgattc ggcgcaacca gaagggtgga tctatcccga cacctaccac
     5641 taccgcaagg gagagagtga tgtgcaactg ctgcgacgtg cccatcagag catgaaaaag
     5701 cggctggccg agcagtggag cgggcgggcg accgacctgc cgctcaaatc gccctatgag
     5761 gcgctgattc tggcctcgat cgtcgagaag gagaccggac agggcagcga acgaccgcag
     5821 attgccggcg tcttcgtgcg acggttgcac agtgacatgc cgttgcagac cgacccgacc
     5881 ctgatctacg gtctgggtgc ctgcttcgac ggcaacctga ccctggccga tctgcgccgc
     5941 cccggcccct acaacagcta cctgaacaag gggttgccgc cgacgcccat cggcctgccg
     6001 ggcgaggcgg cgattcaggc ggtgctgcat ccagcggcgg gggatgcgct ctacttcgtc
     6061 gccaagggtg atggcagcca ccacttttcg gccacgctgg aagcgcatca gcaggccgtt
     6121 gaacagtatc aacagcgtca gcgccgtgcc gattaccggt cggcgccgcg aaatggcggg
     6181 cagtaagatg cagcggggcc gtttcatcac catcgaaggc agcgagggcg ccggcaagag
     6241 cagttgcctg cgcaagatct ccgcgctgtt gcagcagctg tcgatcgagc atcgcatcac
     6301 ccgcgaaccg ggtggcaccg agctggcgga ggcgatccgc gacctgctgc ttcggccctg
     6361 ggctgagccg gttcagccgt tgaccgaact gctgctgctg tttgccggcc gggcacaaca
     6421 tatccagcag ctgatcgaac cggcgctggc ggccggcatc tgggtggtca gcgaccgctt
     6481 caccgatgcc acctatgcct accagggtgg cgggcgcgca gtgccgcagt cgatgattgc
     6541 acagctggag cagctggtgc agggcgagtt gcgtcccgac ctgacgctgt ttctcgacct
     6601 gccggtggcg gatggcctgc ggcggatcga tgggcgggat ggtgagctgg accgtttcga
     6661 gcgtgaacag atcgacttct tcgaacggat acgggcctgc tatctggcgc gcagcgcggc
     6721 cgaaccggag cgtatccgcc gcatcgatgc ctccgcaccg ctcgatcagg tgacggcagc
     6781 catcgaacag accctgcggc aggcgattca gcgctggagc gcgccatgag cgttccacgc
     6841 gaagcgggtc aggatccgct ggcgccgccg ctgccctggc agcggccgca gtggcaacgg
     6901 ctggtcgagc tgcaacagca gcagaagctg gcccatgcgc tgctgctgag cggtccgcag
     6961 ggaatcggca agcgctgctt tgccgagcgt ctggccacct ggctgctctg ccaccagcca
     7021 gggcgcgagc aggcctgtgg cgcctgccgc agctgcggct ggattcgtgc cggcacccat
     7081 cccgacctgt tctggctgct gccggaatcg ccggcgcggg tgatcaagat cgatcaggtg
     7141 cgcgaagcca acgaattcat gcagttgagc gcccaggccg gccacggcaa ggtggtgatc
     7201 atcgatccgg ccgaccagct caaccgcaat gccgccaatg cgctgctgaa atcgctcgag
     7261 gagccggccg ccgagcgctg gctgctgttg gtcagcagcc gctatggcat gttgctgccg
     7321 accatcgtca gccgctgtca gcggttgacg atggtcgaac ccacggcgcc gcaggcgcag
     7381 gcgtggctgc gtgaccacac cgatgccgaa ctgccgtggg atgaaattct ggctgccagc
     7441 cgcaaccggc cgctgcgcgc gctcgatctg gcgcgggagc cgctctggaa gcagtacaac
     7501 cgggccttga ccctgttgag cgccatctcg actgggcgga gcgatccgct gctgctggcc
     7561 gaagggggcg agcagttcga tcccgaactg ctgctcgact ggtggtgggg ctggctggtc
     7621 gcggcgatcc ggctggccag cggtctggcg gccgacctgc cgctgccgac ggagctgacg
     7681 acccgggccg ggcaggccag tccgctggcg ctgttcgact ttctgaaccg gctggagcag
     7741 gcgcgcacga ttcaggggca gcagatcacg ctgaatcgct cactgctgct gggcgatctg
     7801 gccctgcgct ggcgcgatgc ctttgcacgg cggcagccgt tgcaaatggg ctagactgat
     7861 acaatcgcaa cagttcatgg ttctgatgat gtggaacgca gtatcaaccg ggagatcgac
     7921 cgcatgaagc ctcgtcaggg cagcgggcat ggaattcttt ccctgaccat caaggacaag
     7981 gccgtgctct ttgccgccta catgcccttt gtcaggcatg gtggcctgtt cattcccacc
     8041 tccagaaact accagctgca tgatgaagtg ttcatgctgc tgcaattgat ggaagagccc
     8101 gacaagctgc cagtggccgg caaggtggtc tgggtgacgc ccaagggggc gcagagcaac
     8161 cgcgtcgccg gcattggcgt gcagttcagt ggtcaggatg acaccgcgcg caacaagatc
     8221 gagacctatc tggccggcat gctcgaaacc gaccggccca accacaccct gtgacccgac
     8281 tggccggcag caagccggcg ccgcggattt gtccgatgtt cgtagattcc cactgccatc
     8341 tcgaccgtat cgatctcacc ccccatggcg gctcactgga agctgccatc gcggcggccc
     8401 gcgcggctgg cgtcagcggc attctcaacg tcgctgtcga tctgaacgag tttccccagg
     8461 tgttggcgac cgctgccagc cagccggcgg tgtgggcaac ctgtggtctg catccgctgg
     8521 aggtggcgga gccgctgccg acggtcgagc aactgctggc gttggcggcg gatgcgcggg
     8581 tggtggccct tggcgagacc gggctcgacg gccactacgg cagcgagcag agcgcactgc
     8641 agcaggagag ttttcgtcgt catctgctgg ccagccgggc actgcgcaag ccggtggtgg
     8701 tgcacagccg cgatgcccgg gcgcagaccc tggccctgct cgaagcccat gccgacccgg
     8761 cggtgggcgg cgtgctgcac tgcttcaccg aagattgggc gatggccgag cgtgcgctcg
     8821 agctcaactt ccatgtctcg ttctcgggca tcgtcacctt caggaatgcc gatgcactgc
     8881 gcgaggtggc acgtcgggtg ccgctcgatc ggctgctgat cgagaccgac gccccctatc
     8941 tggcaccgat cccctatcgc ggcaaacgca acgagccgcg ctacctgccg gcggtgggcc
     9001 actttgtggc gcaactgcgc ggcatcgcgc cgcaggcgct ggccgaacag accagcgcca
     9061 acttctgcac cctgttcaga atcgatccgg cgctgctggc ggtggcgacg cggtcatagc
     9121 tcccgcactg ccagtgccct ctggcagtcg cagccctcaa caagatcgga tcactggcga
     9181 c
//
LOCUS       DAORCQ010000092         9141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5288_length_9141_cov_5.287145, whole genome shotgun sequence.
ACCESSION   DAORCQ010000092 DAORCQ010000000
VERSION     DAORCQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5288_length_9141_cov_5.287145"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKI23_09330"
     CDS             <1..295
                     /locus_tag="PKI23_09330"
                     /inference="COORDINATES: protein motif:HMM:NF028497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNN37179.1"
                     /translation="WTPKGFVSQQHPTDNQVETQPSLLHSELFSDGLYAFSVFVETLD
                     QNETAQDGRRQRGATSAVTSRIPYRGESVMVTVVGEIPLETASRIARSVAPSP"
     gene            411..1781
                     /locus_tag="PKI23_09335"
     CDS             411..1781
                     /locus_tag="PKI23_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNN37180.1"
                     /translation="MTLNVQAAELPDFSPLVEQYSNAVVNVSSVTKAPRNAALPFQGQ
                     GPYGELPEEAQEFFRHFFGAPPQGGPMQKRASTGSGFIISSDGYVLTNNHVIDGADEI
                     TVRLRDRREIEAKVVGTDPGTDLALLKIDGKDLPVVKLGNSDRLKVGEWVLAIGSPFG
                     FDYSVSAGIISALNRSLPNEQNQNYVPFIQTDVAINPGNSGGPLFNMDGEVIGINSMI
                     YTRSGGFMGLSFAIPISVAMDVVGQIKEKGHVLRGWLGVSIQEVNRDLAESFGLERPA
                     GALVAAVVPDSPAAKGGLQAGDIILKVDGTAIEFSADLPHLIGRKRPGSTAALEVVRN
                     GKPLDLKVKVGELPSEPEKLAQQGGGGAQPQNRLGILVSDLTAEQLRELELPHGIVIE
                     EVVDGVGAAIGLRPGMVITAIANKPVKSAAEFNLIVKSLPANRLIAMRVLRDGVPLFV
                     TFKLPK"
     gene            complement(1833..2621)
                     /gene="mazG"
                     /locus_tag="PKI23_09340"
     CDS             complement(1833..2621)
                     /gene="mazG"
                     /locus_tag="PKI23_09340"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008029317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNN37181.1"
                     /translation="MSRSLTNMLKTLEIMAHLRDPQQGCPWDVEQDFNTLTPCTLEEA
                     YEVVDAIERNDFAGLQEELGDLLLQIVFYCQIASERGLFDFEAVAGSLNDKLIRRHPH
                     VFAQAERTTAQGQQLLWDEIKRQEKAGQPEQGLLDSVPANLPALAEAQKLQKRAAKVG
                     FDWPDAEPVFAKLHEEVDEVREAMALADSAACLEEVGDLLFVVTNLARHLRVDAQQAL
                     SHANHKFRRRFGQMEQMARVMGQGLEMLSLEQQEQLWQRAKQSE"
     gene            2801..3556
                     /locus_tag="PKI23_09345"
     CDS             2801..3556
                     /locus_tag="PKI23_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNN37182.1"
                     /translation="MFEWIADPSAWLALVTLTVLEIVLGIDNIIFISILVGRLPESMR
                     RRARTLGLALAMVTRIALLFSITWVMKLAEPLLTVMEQSFSGRDLILLGGGLFLLWKS
                     SVEIYNSLEGEEEEAHAPTVNLKLGIVSIMIQIALIDIIFSLDSVITAVGLANDFFVM
                     ALAIVIAVLMMMFMSHIIADFVDRHPSIKMLALSFLTMIGMALIGEAFQVHIPKGYIY
                     FAMGFSVLVEVLNIRRASRRAVPVKLHHTLPGD"
     gene            complement(3601..5238)
                     /locus_tag="PKI23_09350"
     CDS             complement(3601..5238)
                     /locus_tag="PKI23_09350"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNN37183.1"
                     /translation="MSDLVRLAAVALLLLGVTLQGASVVAEQDDDAVAAGIARLLSGQ
                     QPELRGFYQARAGRPAWPDDQAVAALAAAIGTLDGDGLRPTDYHPTALLPAYRLAQAA
                     AEPLARARFDLQLSELLLTVLHHVQRGKLDPAAIDPNWELPRAAPRLDLAAVSQAVDA
                     RQFDQLIAAVRPDHLLHARLRSALKHYRDIKLQGGWPMLPARHDALHPGERDADVPLL
                     RQRLAIEEGAALPVSVGCNPAEAAVGGPDPYCHDENLVAAVHRFQRRHLLREDGIVGR
                     QTLAALNVPVATRIEQIRANLERARWLLHGLPRTLVLVDIAGYRLSYFRPDGTLWQSR
                     IMVGRPYRSTPMLRSEINRLTFNPTWTVPPTILREDMLPMIRKDPDYLTREDIEVISL
                     SGAPLDPRQIDWWHPGNLLLRQRAGPHNALGRVAIRFPNRHDVYLHDTPAQGLFNLDR
                     RAISSGCIRVANALELARLLLDDEKKWNQAAIDELVASGLTREVALARRVPLILYYWT
                     VAVDAEGVVSFRPDIYQRDAAVLAALNRPLAAAAPSH"
     gene            5468..6112
                     /locus_tag="PKI23_09355"
     CDS             5468..6112
                     /locus_tag="PKI23_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNN37184.1"
                     /translation="MLVGATAFAANSTTLRQPPLDHLLTRLAPGADPAMLRLAAKALS
                     CAAPRADRLAVIDYSLPSSEPRLWVFDLARQRLLFKERVAHGRGSGDLQASLFSNRPE
                     SFQSSLGLFRTLESYHGRNGYSLRLEGLEPGINDRAYQRAIVIHGAGYVSDTFISRTG
                     RLGRSHGCPAVRLEITQPLIDSIKDDQYLFAYYPDRNWLKESTLLHCQHSLSQR"
     gene            complement(6300..6386)
                     /locus_tag="PKI23_09360"
     tRNA            complement(6300..6386)
                     /locus_tag="PKI23_09360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6350..6352),aa:Leu,seq:cag)
     gene            6615..8192
                     /locus_tag="PKI23_09365"
     CDS             6615..8192
                     /locus_tag="PKI23_09365"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HNN37185.1"
                     /translation="MLALNDRTPRAAALSRFGMPTLCASAILLTAANAHAFQFKFEDP
                     QMKGFLDTSTTLNATWRTQKADARRVSYGNQNFSEGDLVSTPLKITPELGLSYGSMGL
                     FTRANYVYDAKIEGNERISDAAEDVVSNKFRLLDAFVYDSFEFSNAKLTTRLGSQVVN
                     WGESTFIGNGINSTNPIDATKARGAGVEVKEVNLPLPMLWASMDFGGAFSIEAYYNSE
                     WNETILDPVGTFFSTSDAVGEGPNRLRISSALSLPRRNDMLPDDGGAYGVALRTVIEP
                     LNSAEVGLYHLRYHSFAPYVQVTRPGASPLSAYYQLLYPEDIDLYGLSINADLPGDLG
                     ISIGGELSFRPDAPLARDSAVVIGRSLAGAAAVPPIAPYDEGNLTQAQFTLTYQVGSE
                     NPFAADKMTLVFEPGVALANLPDKTYYSNYDSVSWGYAFRGALEYADAFLNMTVTPTL
                     AFRHDVAGTTPGAPSGTFLNDRKSATLSAQAVYLDYLTFELGYTRNWGAEFRSASALI
                     NTAADRDFLALSAKYNF"
     gene            8221..>9141
                     /locus_tag="PKI23_09370"
     CDS             8221..>9141
                     /locus_tag="PKI23_09370"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNN37186.1"
                     /translation="MNKKEFLPIGLAAALSLLPATLLAKATPEELKKIGLEGTELTPV
                     GAIRAGNADGSIPAWTGGLPQKDVPKGQWLDNPFAADKPLFTITAQNYKQYADKLSPG
                     QVAMFERYPDYKMNIYPSRRSASFKPHIYEAALYNAANAEMNPSGIDFHGGKITWAFP
                     IPHTANEAMMSYSTKPREAGTRRWANTTVVTSTGDYQTSKIIEEIISDWSKEGNTPET
                     MTGENDVFYFLQSVMAPPKTAGGVILILDGIHQDGQARRIWSYNPGQRRVRRAPQVQY
                     DNPKTGGDGLATSDQLGMYNGKIDRYDWKLV"
BASE COUNT         1782 a         3107 c         2669 g         1583 t
ORIGIN      
        1 ctggacaccc aagggttttg tctctcagca gcatcccaca gacaaccagg tcgagaccca
       61 gccgagcctg ctgcacagcg aactgttcag cgatgggctc tatgccttct cggtctttgt
      121 cgaaaccctg gaccaaaacg agacggcaca ggatggccgc cgccaacgcg gtgccacctc
      181 ggcggtcacc agtcgaattc cctaccgcgg cgagagcgtg atggtcacgg tggtcggcga
      241 aattccgctg gagaccgcca gtcgaatcgc ccgctccgtg gcgccttccc cctgaatctc
      301 atggatcatc caccatcttc cactgcacaa gaggaataga aatggcgaat gcaatcaagg
      361 aactgcgtcc cgttggtgtt ctgtttgcca cgctctgcct ggccgcgctg atgacactga
      421 atgtccaggc cgccgaactg cccgacttct cgccattggt cgaacagtac tcgaacgccg
      481 tggtcaacgt cagttccgtc accaaggcac cgcgcaacgc ggccctgccc tttcagggac
      541 aagggcccta tggcgaactg cccgaagagg cccaggagtt cttccggcac tttttcggcg
      601 ccccgccaca aggcggaccg atgcagaagc gcgcctcgac cggttcaggc ttcatcatct
      661 cctccgatgg ctatgtcctg accaacaacc acgtcatcga tggcgctgac gaaatcaccg
      721 tcagactgcg ggatcggcgg gagatcgagg ccaaggtggt gggcaccgac cccggcaccg
      781 atctggcact gctcaagatc gatggcaagg atctgccagt ggtcaagctg ggcaattccg
      841 accggctgaa ggtcggcgag tgggtgctgg ccatcggctc gcccttcggt ttcgactact
      901 cggtctcggc cggcatcatc agcgcgctga accgcagcct gcccaacgag cagaatcaga
      961 actacgtgcc cttcatccag accgatgtcg ccatcaaccc cggcaactcc ggtggcccgc
     1021 tgttcaacat ggatggcgaa gtgatcggca tcaactcgat gatctacacc cgctccggcg
     1081 gtttcatggg gctgtcgttc gccattccga tctcggtggc gatggatgtg gtgggtcaga
     1141 tcaaggagaa aggccatgtg ctgcgcggct ggctcggcgt cagcatccag gaggtgaacc
     1201 gcgacctggc ggaatcgttc ggactggagc gcccggccgg ggcgctggtg gccgccgtgg
     1261 tgccggacag tccggcggcc aaggggggcc tgcaggccgg tgacatcatc ctcaaggtcg
     1321 atggcactgc gatcgaattc tcggccgacc tgccccatct gatcgggcgc aagcgccccg
     1381 gttcgaccgc ggcactggag gtggtgcgca acggcaagcc gctcgatctg aaggtcaagg
     1441 tgggtgaact gccgagcgaa ccggagaagc tggcccagca ggggggcggc ggagcgcagc
     1501 cgcagaaccg cctcggcatc ctggtgagcg acctgactgc ggagcagctg cgtgaactgg
     1561 agcttcccca tggcatcgtg atcgaggagg tggtcgacgg tgtcggtgcg gccatcgggc
     1621 ttcgccccgg catggtgatc accgccatcg ccaacaagcc ggtcaagagt gccgccgagt
     1681 tcaacctgat cgtcaagtcg ctgccggcaa accggctgat tgcgatgcgg gtgctgcgcg
     1741 acggcgtgcc gctgttcgtg accttcaaac tgccaaagta atccacactt tcgccggggg
     1801 atgggccgcg cggcagcgcg cccatccgtc gatcactcgc tctgcttggc gcgctgccag
     1861 agctgctcct gctgctcgag cgacagcatc tccagcccct gccccatcac cctggccatc
     1921 tgttccatct gcccgaagcg gcggcggaac ttgtggttgg catggctcag cgcctgctgt
     1981 gcatccaccc gcagatggcg cgccagattg gtcaccacga acagcagatc acccacctcc
     2041 tccaggcagg cggccgaatc agccagcgcc atcgcctcac gcacctcgtc gacctcttca
     2101 tgcagtttgg caaataccgg ctcggcatcg ggccagtcga aaccgacctt ggcagcacgc
     2161 ttctgcagct tctgcgcctc ggcgagtgcc ggcaggttgg ccggcaccga atcgagcaga
     2221 ccctgctcgg gttgcccggc cttctcctgc cgcttgatct cgtcccacag cagctgctgc
     2281 ccctgcgccg tggtccgctc cgcctgtgca aagacatggg gatggcggcg gatcagcttg
     2341 tcgttcaggc tgccggccac cgcctcgaaa tcgaacaatc cgcgctcgct ggcgatctgg
     2401 cagtaaaaga cgatctgcag cagcagatcg cccaactcct cctgcaaccc ggcaaagtca
     2461 ttgcgctcga tcgcatcgac cacctcatag gcctcttcga gcgtgcaggg ggtcagggtg
     2521 ttgaaatcct gctcgacatc ccagggacag ccctgttgcg gatcgcgcag gtgtgccatg
     2581 atttcaagcg ttttcagcat gttggtcaaa gatcggctca taagggctct gcgatggggg
     2641 gatggcaggg gcgtcaatta ttgccctcct ggtcgacgct gacaaaccgg aaacaggcct
     2701 gaaaggccca gccccgcgca ctgctgcttt gagcacaatg cggcttgcgg taaagtggcg
     2761 accttcaccg tacatcattc aaccgcgagt gcagtcgttc atgttcgaat ggatcgccga
     2821 tccgagtgcc tggctggcgc tggtcaccct gaccgttctc gaaatcgtgc tgggcatcga
     2881 caacatcatc ttcatcagca tcctggtggg gcgcctgccc gagtcaatgc gccgccgcgc
     2941 ccgcaccctg gggctggcgc tggcgatggt gacccgcatc gcgctgctgt tctcgatcac
     3001 ctgggtgatg aagcttgccg aacccctgct gacggtgatg gaacagagct tctcgggccg
     3061 cgacctgatc ctgctgggcg gtggactctt tctgctgtgg aaaagcagcg tggagatcta
     3121 caacagcctc gaaggcgaag aggaagaggc gcacgcaccg accgtcaacc tgaagctggg
     3181 cattgtttcc atcatgatcc agatcgcact gatcgacatc atcttctcgc tcgactcggt
     3241 gatcaccgca gtcggcctcg ccaacgactt cttcgtgatg gcactggcga tcgtcatcgc
     3301 cgtgctgatg atgatgttca tgtcgcacat catcgccgac ttcgtcgacc gccatcccag
     3361 catcaagatg ctggcgctga gctttctgac gatgatcggc atggcgctga tcggcgaggc
     3421 cttccaggtc cacattccca agggctacat ctatttcgcg atgggcttct cggtgctggt
     3481 cgaggtgctg aacatccgcc gggcctcacg ccgcgccgtt ccggtcaagc tgcaccacac
     3541 ccttcccggc gattgaaccg cacgcaacaa gcggcaaaaa gccagggccg ggccccaccc
     3601 tcagtgtgac ggcgctgctg cggccagcgg ccggttcagc gccgccagca ctgccgcgtc
     3661 gcgctggtag atatcgggac gaaaactcac cacaccctcg gcatcgaccg ccaccgtcca
     3721 gtagtacaga atcagcggca cgcgtcgggc cagcgcgact tcacgcgtca acccactggc
     3781 gaccagctca tcgatcgcgg cctggttcca ctttttctca tcgtcgagca gcagacgggc
     3841 cagctccagc gcattggcaa cccggatgca acccgagctg atcgcgcgcc gatcgaggtt
     3901 gaacagcccc tgcgccggcg tgtcgtgcag atagacatca tgccggttgg gaaaacggat
     3961 cgccacccgc cccagcgcat tgtgcggacc ggcacgctgc cgcagcagca gattgcctgg
     4021 atgccaccag tcgatctgtc gtggatcaag cggcgctcca gacagcgaaa tgacctcgat
     4081 gtcctcccgc gtcagataat ccggatcctt gcggatcatc ggcagcatgt cctcgcgcag
     4141 gatggtgggc ggcaccgtcc aggtcggatt gaaggtcagg cggttgatct ccgagcgcag
     4201 catcggtgta ctgcggtagg gccgccccac catgatgcgg ctctgccaga gcgtcccatc
     4261 ggggcgaaaa tagctcaacc ggtaaccggc gatgtccacc agcaccaagg tccgcggcag
     4321 gccatgcagc agccagcggg cccgctccag attggcccgg atctgctcga tgcgtgtcgc
     4381 caccggcaca ttcaacgccg ccagcgtctg cctgccgacg atcccatcct cccgcagcag
     4441 gtggcggcgc tggaaacggt gcaccgcagc gaccaggttt tcatcatggc agtatgggtc
     4501 cgggcccccg accgctgcct ccgcagggtt gcagccaacc gataccggca gtgcggcgcc
     4561 ctcctcgatc gccagtcgct gacgcaacag cggaacatcg gcatcccgct caccaggatg
     4621 cagtgcgtcg tgacgggccg gcagcatcgg ccagccaccc tgcagcttga tgtcacgata
     4681 atgcttcagg gcggaacgca accgcgcatg gagcagatgg tccggccgca cagcggcaat
     4741 gagctgatcg aactgccggg catcgaccgc ctgcgacacc gcagccagat cgagccgcgg
     4801 tgccgcgcgc ggcagctccc agttcggatc gatcgccgcc ggatcgagct tgccacgctg
     4861 cacatgatgc agcaccgtca gcaacagttc gctcagttgc agatcgaagc gtgcccgcgc
     4921 cagcggctca gccgccgcct gcgccagccg ataggccggc agcagtgccg tcgggtggta
     4981 atcggtcggt cgcaggccat cgccatcgag ggttccgatg gccgccgcca aggcagccac
     5041 cgcctggtca tcaggccatg ccggccgtcc agcacgcgcc tgataaaagc cgcgcaattc
     5101 aggctgttgc cccgacaaca atcgggcaat gccggctgcc accgcatcgt catcctgctc
     5161 ggccaccaca ctggcaccct gcagggtcac acccagcagc aggagcgcga cagcggccag
     5221 cctgacgaga tcgctcatca gatgccttgc cggatcgaca gaaaaatttg gccgctccgc
     5281 tgcccctgtc gataatggtg ctcgccgctg ccgcagtaca cccatgccgt gcgctctgtc
     5341 caacccaaag acccggatcg caagaccctc atggatgacg gcctcacgca gaacagagga
     5401 tgacgcttcg atgacatgtc ccaggccatt tctgtcatgg tgccgcacgg tgctgaccac
     5461 cctgctgatg ctcgtgggcg ctacagcctt cgcggccaac tccaccaccc tgcggcaacc
     5521 gcctctcgac cacctcctga cccgcctggc acccggtgcc gacccggcca tgttgcggct
     5581 ggccgccaag gccctctcct gcgccgcgcc acgggccgac cggctggcag tgatcgacta
     5641 ctcgctgccc tcctccgaac cgcgcctgtg ggtcttcgat ctggcccggc agcggctgct
     5701 gttcaaggag cgggttgcgc atggtcgcgg ttccggtgac cttcaggcca gcctcttttc
     5761 caacaggcct gaaagttttc aatcgagcct ggggctgttc cgcacgctgg agagctacca
     5821 tgggcgcaat ggctactcat tgcgactgga gggactggag cccggcatca atgaccgcgc
     5881 ctaccagcgc gcgatcgtca tccatggcgc cggctatgtc agtgacacct tcatcagccg
     5941 gaccggacgc ctgggtcgca gccatggctg cccggcagtg cgactggaga tcacccagcc
     6001 actgatcgac agcatcaagg atgatcaata cctgttcgcc tactatccag accgcaactg
     6061 gctgaaagag tccaccctgt tgcactgcca gcacagcctg tcgcaacgct gagccattcg
     6121 gatcggacca cactgcgcct ctggccgacg cggccgaaaa ccccatcgca atgccgacgc
     6181 cggaacgtca aggccgccct gctggtcgtc aaggtaaatg ggggtggaac ccaacccgca
     6241 atcgtgagct ggagcggacc atggagattt cagatgctgc tgtggatgca gatcacggtt
     6301 ggtgccgagg agaggacttg aacctccacg gggtttcccc cactagcacc tgaagctagc
     6361 gtgtctacca atttcaccac ctcggcaatc agacgccatg gcgtgaccat gtgcccgagg
     6421 cgcgcaatct accggcgccg ccgcaccttg tcaacaatgc aacccgtcgt ccccctgccg
     6481 caaggccctt tcgacgacaa tcgctctgga aatcagccaa aaactgcgca tccggcttgc
     6541 gcgatgcgcc aaccgcctat accctcggca ccgtcgatga caccaagtcg ataacatcaa
     6601 taaggagaga cttcatgctc gcactcaatg acaggacgcc tcgcgccgcc gcgctgagca
     6661 gattcggcat gccgaccctc tgcgccagcg cgattctgct gacagcggcc aacgcccacg
     6721 cctttcagtt caagttcgaa gacccgcaga tgaagggctt cctcgacacc tcgaccaccc
     6781 tcaatgccac ttggcgtacg cagaaagcag acgcacgcag ggtcagctat ggcaaccaga
     6841 atttcagcga aggcgacctg gtctccaccc cgctcaagat cacgccggaa ctgggcctga
     6901 gctacggctc gatgggtctg ttcacccgcg ccaactatgt ctatgacgcc aagatcgaag
     6961 gcaacgagcg catctccgac gcggccgagg atgtggtcag caacaagttc cgcctgctcg
     7021 acgcctttgt ctatgacagt ttcgagttca gcaacgccaa actgaccacc cgcctcggca
     7081 gccaggtggt caactggggc gagagcacct tcatcggcaa cggcatcaac agcaccaacc
     7141 cgatcgatgc caccaaggcc cgaggcgccg gtgtcgaagt gaaggaggtg aacctgccgc
     7201 tgccgatgct ctgggcatcg atggattttg gcggcgcgtt cagcatcgag gcctattaca
     7261 actcggagtg gaacgagacc atccttgacc cggtcggcac cttcttcagc accagtgatg
     7321 cagtcggtga aggccccaat cgtctcagaa tctcctctgc gctctctctg ccgcgcagaa
     7381 acgacatgct gccagatgat ggcggcgcct atggagtggc attgcgcacg gtgatcgagc
     7441 cgctcaacag cgccgaggta ggcctgtacc acctgcgcta ccacagtttt gccccctatg
     7501 tgcaggtgac cagaccgggc gccagccccc tgagcgccta ttaccagctg ctctatccgg
     7561 aagacatcga cctttatggt ctcagcatca atgccgacct gccgggcgac ctgggcatct
     7621 cgataggcgg cgagctctcc ttccgccccg atgcaccgct ggcacgtgac agcgccgtgg
     7681 tgatcggaag aagcctcgct ggtgcagccg ccgttccacc gatcgccccc tatgacgaag
     7741 gcaacctgac ccaggcgcag ttcacgctga cctatcaggt cggttcagag aaccccttcg
     7801 ccgccgacaa gatgacactg gtgttcgaac cgggcgtcgc gctggccaac ctgccggaca
     7861 agacctacta cagcaactac gactcggtct cctggggcta tgcattccgt ggcgcccttg
     7921 agtatgccga tgcctttctc aacatgacgg tcaccccgac actggccttc cgccacgacg
     7981 tcgccggtac cacccccggc gcgcccagtg gcaccttcct caatgaccgc aagtcggcca
     8041 cgctgtcggc acaggcggtc tacctcgact atctgacctt cgagttgggc tacacccgca
     8101 actggggcgc tgaattcaga tcggccagtg cactcatcaa tacggccgct gaccgcgact
     8161 tcctcgccct cagcgccaaa tacaacttct gacccgcagc aataccgagg actctcaacc
     8221 atgaacaaga aggaattcct gcccatcggc ctggcagcgg cactgagcct gctgcctgcc
     8281 acgctgctgg ccaaggccac ccctgaagag ctgaagaaga tcggcctgga gggcaccgag
     8341 ctgaccccgg tcggagcgat tcgcgccggc aacgccgatg gcagcatccc ggcctggacc
     8401 ggcggtctgc cgcagaagga tgtgcccaaa gggcagtggc tcgacaaccc ctttgccgcc
     8461 gacaagccgc tcttcaccat caccgcgcag aactacaagc agtatgccga caagctgagc
     8521 cccggtcagg tggcaatgtt cgagcgctac cccgactaca agatgaacat ctacccatcg
     8581 cggcgctcgg cctcgttcaa gccccacatc tacgaggcag cgctctacaa cgccgccaat
     8641 gccgagatga acccgtcggg catcgacttc catggcggca agatcacctg ggccttcccg
     8701 attccgcaca ccgccaacga agcgatgatg agctacagca ccaagccgcg ggaggccggt
     8761 acccgccgct gggccaacac caccgtggtg acctcgaccg gcgactacca gaccagcaag
     8821 atcatcgagg agatcatcag cgactggtcg aaggagggca acacccccga gaccatgacc
     8881 ggagagaatg atgtcttcta cttcctgcag tcggtgatgg caccacccaa gaccgccggc
     8941 ggtgtgatcc tgatcctcga cggcatccac caggatggtc aggcacggcg catctggtcc
     9001 tacaaccccg gtcagcgccg cgtgcgccgc gcgccgcagg tgcagtacga caaccccaag
     9061 accggcggtg atggcctggc caccagtgac cagttgggca tgtacaacgg caagatcgac
     9121 cgctacgact ggaagctggt c
//
LOCUS       DAORCQ010000093         9045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5361_length_9045_cov_5.653059, whole genome shotgun sequence.
ACCESSION   DAORCQ010000093 DAORCQ010000000
VERSION     DAORCQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5361_length_9045_cov_5.653059"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /locus_tag="PKI23_09375"
     CDS             complement(<1..1997)
                     /locus_tag="PKI23_09375"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNN37187.1"
                     /translation="MLESYRKHVEERAAEGVPPKPLDAQQVAALVELMKSPPPGEGDF
                     LLELLSQRVPPGVDEAAYVKAGFLAAIAKGETSSPLIEPVKAVELLGTMLGGYNVEPL
                     IQLLDDARLGPTAAKALSHTLLIFDSLHDVLEKARAGNANARQVVDSWAAADWFTARP
                     ELAHKLTMTVFKVDGETNTDDLSPAGDAWSRPDIPLHAKAMLVKRMENPLGKIEELKQ
                     KGHPLVYVGDVVGTGSSRKSATNSVLWLMGNDIPHVPNKRGGGVCIGGKIAPIFFNTM
                     EDAGALPFECDVTGLKMGDVIDIHPYEGVIKAHDSDRVITRFALKSEVLLDEVRAGGR
                     IPLIIGRGLTDRVRRELGLPPSTLFRRPTAAADSGKGFTLAQKMVGRACGVAGVRPGV
                     YCEPHMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPRPVDIETQHTL
                     PDFIMNRGGVSLRPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFA
                     AATGVMPLDMPESVLVRFKGQMQPGITLRDLVNAIPYAAIQQGLLTVPKEGKKNVFSG
                     RILEIEGLPQLKVEQAFEISDASAERSAAGCTIKLDKAPIIEYLNSNVVMLKWMLANG
                     YGDVRTIERRIAAMQAWIADPQLLSADADAEYAAVIEIDLN"
     gene            2337..3776
                     /locus_tag="PKI23_09380"
     CDS             2337..3776
                     /locus_tag="PKI23_09380"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015487206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNN37188.1"
                     /translation="MNPQHEALFDEWKNSEAIAEAMIPLIGRLYRENNIVLSIYGRAL
                     VNKTVIDVLRLHRFARRVTSNPLSVADSFPILKVLTELDLGPARIDLGRLLDKYNGSE
                     PVEQFVRREMAPIIGRNAPPIPQPQDVVLYGFGRIGRLLARLLIEKAGGGDLLRLRAI
                     VVRKGKGGDLEKRASLLRRDSIHGPFNGSIVPDEKNSGLLVNGNFIQVIYASSPAEVD
                     YASYGISNAIVVDNTGIWRDQEGLSQHLKCPGVGRVLLTAPGKGEIKNVVFGINQDVI
                     GKEQIVSAASCTTNAIVPVLKVMNDNFGVESGHIETVHSYTNDQNLLDNYHSGSRRGR
                     SAPVNMVITETGAAKAVAKALPELAGKLTGNAIRVPTPNVSLAVINLNLAKESSVEAV
                     NALLHDAALNSSLRKQIDYVDSSEVVSTDFIGSRHAGVVDAKATIVSGNHCVLYVWYD
                     NEFGYSCQVVRVLQHMAGISYITLPAGSN"
     gene            3853..4359
                     /locus_tag="PKI23_09385"
     CDS             3853..4359
                     /locus_tag="PKI23_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNN37189.1"
                     /translation="MSALVWIDWTILGVITLSALLSLTRGFMREAVSLLSLLVAVVVA
                     RLFSGQMELLLAGWIASAPLRTVAAFALLFFGVLVMGGILGRLLGHLLRVSGLGMFDR
                     LLGMAFGVVRGLIIVVLLVAAIEQTPATSSESWHSSRLLPHLLLLADWGRHTLLPLVT
                     GTITAATG"
     gene            4440..5954
                     /gene="purF"
                     /locus_tag="PKI23_09390"
     CDS             4440..5954
                     /gene="purF"
                     /locus_tag="PKI23_09390"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNN37190.1"
                     /translation="MCGVVGMVAHSNVNQGLYDALTVLQHRGQDAAGIMTSADGRMHL
                     RKGNGLVRDVFQERDMQMLLGNVGIGHVRYPTAGSSSVALAQPFYVNSPYGISLAHNG
                     NLTNTAKLSQELYRDDLRHVNTDSDSEVLLNVLAHELQAHATHTPTPEVFFRAVAGVQ
                     RRCRGGFAVVALVMGHGVLGFRDPNGIRPLVVGRRETEQGVEHMIASESVALDALDFK
                     LLRDVLPGEAVFIDLHGELHSQLCATQTHYSPCIFEHVYFARPDSIIDGISVYKARLR
                     MGEKLAEKILRSWPDHDIDVVIPIPDTSRTSALPLANRLGVKFREGFVKNRYIGRTFI
                     MPGQSQRKKSVRQKLNAIDLEFRGKNVLLVDDSIVRGTTCQQIVQMARDAGARKVYFA
                     SASPPVRYPNVYGIDMPAATELIAHGRSEAEVQRLIGADRLIYQDLKDLIACAEEGNP
                     KINTFECAVFTGNYVTDDVDELYLARIQATRNDAMKARMEAELANGQAYGDRMM"
     gene            5996..7210
                     /locus_tag="PKI23_09395"
     CDS             5996..7210
                     /locus_tag="PKI23_09395"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNN37191.1"
                     /translation="MNDELHSRAEGPVQLESTRFDTLAVRAGQTRTAEGEHGEPIFTT
                     SSYLFENASNAAERFAGITPGNVYSRYTNPTVRMFEERLAALEGAEQAVGTASGMAAI
                     LSLMMTLLQAGDHVICSASVFGTTVGLFNRYLRKFGVETTFVHLTHLDDWRAAVRPNT
                     RLLFLETPSNPLCEVADLAALSTLARQIGAKLVVDNCFCSPALQRPLELGADYVVHSA
                     TKYLDGQGRCVGGVVAGPASVMSEVVAFLRTAGPTMSPFNAWVFLKGLETLRLRMDAH
                     SANALTLARWLQSQPGIEQVYHCGLPTHPQHQLALRQQRGFGGVFSFSVSGDRAAAWR
                     FIDATRIFSLTANLGDAKSTIIHPATTTHGRLSAEERTNAGIGDNLIRLAVGLEAVED
                     LQDDLARGLAAL"
     gene            7263..8198
                     /locus_tag="PKI23_09400"
     CDS             7263..8198
                     /locus_tag="PKI23_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN37192.1"
                     /translation="MQKLVEQIIAEVGRVVLGKERQVRLALGCLIAGGHLLIEDLPGM
                     GKTTLAQTLARVLGLEFSRIQFTSDLLPADILGVSLFSPQTQQFNFRPGPLFAQLVLA
                     DEINRATPKTQSALLEAMEEQQATVDGVSHPLPQPFFVIATQNPVTQLGTFALPESQL
                     DRFLMRIDIGYPHPAVERRMLAGEMPRQLLEHLAPLGDATTLLRLRQAVRQIKASDAL
                     LDYVQALIAFSRNAGHFVNGLSPRGAQAMMESARAWALLAGRDYLLPEDIQAILPSVV
                     DHRLQTIERDAALPSALMVAAVDPLARQRGGALRP"
     gene            8205..>9045
                     /locus_tag="PKI23_09405"
     CDS             8205..>9045
                     /locus_tag="PKI23_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNN37193.1"
                     /translation="MRWRRPRLPDRLQKGYDRWLARRFPPLSRLRLGHRQLFIVPSLS
                     GGLFMLLLAAMLLAAINYQNSLAFALSFWLLGLMPIALIHAYRNLLGLELTALGSTAG
                     FVGGHGQLRLQLRAERPLRHAIELRLGGGEWVSDADSEERSITLTLPLASRGWQAIPR
                     LEVRSGFPLGLFRVWSYPCLDWKLLAYPHPFAADAMPPVSGESSAALPHGVQSGDEDF
                     AGLDPWRLGDGWGRVAWKQWARRGELLVKRLEQPEQGAQWLDFDHCPDRSIEARLSRL
                     AGWV"
BASE COUNT         1582 a         2795 c         2934 g         1734 t
ORIGIN      
        1 tggttcagat cgatctcgat caccgcggca tactcggcat cggcatcggc cgacagcagt
       61 tgcggatcgg caatccaggc ctgcatcgcg gcaatgcgcc gctcgatggt gcggacatcg
      121 ccatagccgt tggccagcat ccacttcagc atcaccacgt tggagttgag gtactcgatg
      181 atcggcgcct tgtcgagctt gatcgtgcag ccggcagcgg agcgctcggc tgaggcatcg
      241 gagatctcga acgcctgctc gaccttgagc tgcggcagac cttcgatttc gagaatgcgg
      301 ccggagaaga cattcttctt gccctccttc ggtacggtca gcagaccctg ctggatggcc
      361 gcgtagggga tggcattgac cagatcacgc agcgtgatgc ccggctgcat ctggcccttg
      421 aagcgcacca gcaccgactc gggcatgtcg agcggcatca ccccggtggc ggcggcaaag
      481 gcgaccagac cggagccggc tggaaaggag atgccgatcg ggaagcgggt gtgggagtcg
      541 ccaccggtgc cgacggtgtc gggcagcagc atccggttga gccaggagtg gatgatgcca
      601 tcgcctggac gcagcgagac accgccgcgg ttcatgatga agtcgggcag ggtgtgctgg
      661 gtctcgatgt cgaccggccg gggataggcc gcggtgtgac agaacgactg catcaccagg
      721 tcggcggaaa agccgaggca ggccagatcc ttcagctcat cacgggtcat cgggccggtg
      781 gtgtcctggg aaccgaccgt ggtcatgtgc ggttcgcaat agacgccggg gcgcacgccg
      841 gcaacgccac aggcgcgacc gaccatcttc tgtgccaggg tgaagccctt gccgctgtcg
      901 gccgccgcgg tgggacggcg gaacagcgtg ctgggcggca ggcccaattc acggcggacg
      961 cggtcggtga ggccgcggcc gatgatcagc gggattctgc ctccggcacg cacttcgtcg
     1021 agcagcacct ccgacttcag cgcaaaacgg gtgatcaccc tgtcgctgtc atgggccttg
     1081 atcacccctt catagggatg gatgtcgatc acatcaccca tcttcagacc ggtcacatcg
     1141 cactcgaagg gcagggcgcc ggcatcttcc atggtgttga aaaagatcgg ggcgatcttg
     1201 ccgccgatgc agacgccacc gccgcgcttg ttgggtacat ggggaatgtc attgcccatc
     1261 agccacagca ccgagttggt ggccgacttg cgcgaggagc cagtgccgac cacatcgccg
     1321 acatagacca gcggatggcc tttctgcttc agctcctcga tcttgccgag cgggttctcc
     1381 attcttttga ccagcatcgc cttggcgtgc agcgggatgt ccggacggct ccaggcatcg
     1441 cctgccggcg agaggtcatc ggtgttggtc tcgccatcga ccttgaaaac ggtcatggtg
     1501 agcttgtgtg ccagctcagg acgggcagtg aaccagtcag cggcggccca gctgtcgacc
     1561 acctggcggg cattggcatt gccggcacgg gctttttcga gcacatcgtg cagcgagtcg
     1621 aagatcagca aggtgtgaga gagtgccttg gccgcagtgg gccccagcct ggcatcatcg
     1681 agcagctgga tcagtggctc gacgttgtag ccacccagca tggtgccgag cagctcgacc
     1741 gccttcaccg gctcaatcag cggcgagctg gtttcgccct tggcgatggc tgccagaaaa
     1801 ccggccttga cgtaggcagc ctcatcgacg ccgggtggca cccgttggct gagcagttcc
     1861 agcagaaaat cgccttcgcc cggcggtggc gacttcatca gttcgaccag cgcggcaacc
     1921 tgttgcgcat ccaggggttt tggagggaca ccttcggccg cacgctcttc gacatgtttt
     1981 ctgtaggatt caagcacctg tgaagacctc ttgagatgat ttcagatttg atgggcaatt
     2041 ttggctgtgg aaggcgaacg ggtggcgcat gattcaaagc gctctttgca gggtaaacac
     2101 cgtttttcag tgtaaagcac cacccgattt ttgttaagca aggggagagt gtcctggacc
     2161 ggttgcaagg ctgtggattg cctctgtcgg tgtcgatcaa agataattgc cggctatgct
     2221 ggtaatgagt gtggtggacg gaattttgtg ccgccgccgt gatcgaatca gacagcgtcg
     2281 ccaagccagg tggcgatgtg ccaacccaat tttcatccaa caggtgtccg tccgtgatca
     2341 atccccaaca tgaagcgctc ttcgatgaat ggaaaaacag cgaggccatt gccgaggcga
     2401 tgattccgtt gattggccgg ctctaccgcg agaacaacat cgtgctgtcg atctatggac
     2461 gggcgctggt caacaagacg gtgatcgatg tcttgcgcct gcaccgtttt gcccggcggg
     2521 tgaccagcaa cccgttgtcg gtggccgaca gttttccgat cctgaaggtc ctgaccgagc
     2581 tggaccttgg cccggcccgc atcgacctgg gtcgtctgct ggacaaatac aacggcagcg
     2641 aaccggtgga gcagttcgta cggcgcgaga tggcaccgat catcgggcgc aatgcgccac
     2701 cgattccgca gccacaggat gtggtgctct atggttttgg tcgcatcggt cgactgctgg
     2761 ccaggctgct gatcgaaaag gcaggcggtg gcgatctgct gcggttgcgg gcgatcgtgg
     2821 tgcgcaaggg caagggcggc gatctggaga agcgcgccag cctgttgcgc cgtgattcga
     2881 tccatggtcc tttcaatggc agcatcgtcc ccgacgaaaa gaacagtggc ctgctggtca
     2941 acggcaactt cattcaggtg atctacgcca gctcgccggc cgaggtcgat tatgcttcct
     3001 atggcattag caacgcgatc gtcgtcgaca acaccggtat ctggcgtgac caggaggggc
     3061 tgtcgcaaca tctcaaatgc cccggggttg gccgcgtgct gctgacggca cccggcaagg
     3121 gcgagatcaa gaatgtcgtc tttggcatca atcaggatgt gatcggcaag gagcagatcg
     3181 tctccgccgc ctcctgcacc accaatgcga tcgtgccggt gctgaaggtg atgaacgaca
     3241 acttcggtgt cgaaagtggt cacatcgaga cggtccactc ctacaccaac gaccagaatc
     3301 tgctcgacaa ctaccacagc ggcagccgcc gtggacgcag cgcgccggtc aacatggtga
     3361 tcactgaaac cggtgccgcc aaggcggtgg ccaaggcatt gccggagctg gccggcaagc
     3421 tgaccggcaa cgccatccgc gtgccgacgc ccaacgtctc gctggcggtc atcaacctca
     3481 acctggccaa ggagagttcg gtcgaagcgg tgaacgcact gttgcacgat gccgcgctca
     3541 actcttcgct gcgcaagcag atcgactatg tcgactcatc ggaggtggtg tcgaccgact
     3601 tcatcggttc acgtcatgcc ggtgtggtcg atgccaaggc cacgatcgtc agcggcaacc
     3661 actgtgtgct ctatgtctgg tatgacaacg agttcggtta cagttgtcag gtggtgcggg
     3721 tgctgcaaca catggcgggc atctcctata tcactctgcc agccgggtcg aattgacggc
     3781 gctggcgcgc gtccggtggc agtggatggc cttcgctgct gccggccacc ctgatctgac
     3841 tgaggatgat cgatgagcgc cctggtctgg atcgattgga caatcctggg tgtgatcact
     3901 ctctccgcgc tgctcagcct gacccgtggc ttcatgcgcg aggccgtctc gctgctctcc
     3961 ctgctggtgg ccgtggtggt cgcccggctc ttttcggggc agatggagct gctgctggcc
     4021 ggatggatcg cctccgcgcc actgcgcacc gtcgcggcct tcgcgctgct gttctttggt
     4081 gtgctggtga tgggtggcat cctgggacgc ctgctgggac atttgctgcg ggtcagcgga
     4141 ttgggtatgt tcgatcgcct gttgggcatg gcctttggtg tcgtgcgcgg gttgatcatc
     4201 gtggtattgc tggtggcagc catcgagcag acgccagcga cctcaagcga gtcatggcac
     4261 agctcccggc tgcttcccca tctgctgttg ctcgccgatt gggggcgtca tacgctgctg
     4321 cccctggtga cagggacgat aacggccgca accggctgaa cgacccgaat ggatcgtgac
     4381 gacacggctg ttcgagcgac agcaaacttc aactctgatg gcgacgaccg aggtgtccta
     4441 tgtgcggcgt agttggcatg gttgcacaca gcaacgtaaa ccaggggctc tacgatgccc
     4501 tgacggtgct gcaacaccgt ggtcaggatg cagcgggcat catgaccagc gccgatggcc
     4561 ggatgcacct gcgcaagggc aatggcctgg tgcgtgatgt gttccaggag cgcgacatgc
     4621 agatgctgct gggcaacgtc ggcatcggtc atgtccgcta tcccacggcg gggagctcca
     4681 gtgtggcgct ggcgcagccg ttctacgtca actcgcccta cggcatcagc ctggcgcaca
     4741 acggcaacct gaccaacacg gccaaactga gtcaggagct ctatcgggac gatctgcgcc
     4801 acgtcaacac cgattccgac tccgaggtgc tgctcaatgt gctcgcccat gagttgcagg
     4861 cccatgccac ccacacgccg acgcccgagg tgttctttcg tgccgtggcc ggcgtgcagc
     4921 ggcgctgtcg cggggggttc gccgtggtgg cgctggtgat ggggcatggt gtgctcggct
     4981 ttcgcgaccc caacggcatt cggccgctgg tggtgggacg gcgcgagacc gagcaggggg
     5041 tcgaacacat gatcgcctcc gagagcgtcg ccctcgacgc gctcgacttc aagctgttgc
     5101 gcgacgtgct gcccggcgag gcggtcttca tcgatctgca tggcgaactg cacagtcagc
     5161 tctgtgcgac ccagacccac tacagcccct gcatcttcga gcatgtctac tttgcccggc
     5221 ccgattcgat catcgatggc atctcggtct acaaggcacg gctgcggatg ggtgagaagc
     5281 tggctgaaaa gattctgcgc agctggcccg accatgacat cgacgtggtg attccgattc
     5341 cagacaccag ccgcacttcg gcgctgccgc tggccaaccg cctgggcgtc aagttccgcg
     5401 aggggttcgt caagaaccgc tacatcggtc gcaccttcat catgccgggg cagagccagc
     5461 gcaagaaatc ggtcaggcag aaactcaacg ccatcgatct ggagttccgc ggcaagaatg
     5521 tgctgctggt cgatgactcc atcgtgcgtg gcaccacctg ccagcagatc gtgcagatgg
     5581 cacgcgatgc cggcgcgcgg aaggtctatt tcgcctcggc ctcgccgccg gtgcgctacc
     5641 ccaatgtcta tggcatcgac atgcccgccg ccaccgagct gattgcccat ggccgcagcg
     5701 aagccgaggt gcagcggttg atcggtgccg accggttgat ctatcaggat ctgaaagatc
     5761 tgatcgcctg cgccgaagag ggcaatccga agatcaacac cttcgagtgt gcggtcttca
     5821 ccggcaacta tgtgaccgac gatgtcgatg agctctatct cgcccgcatc caggccaccc
     5881 gcaacgacgc catgaaggcc cggatggagg cggaattggc caacggccag gcctatggtg
     5941 atagaatgat gtaaaggcga tgcgaccatg atcagatcca gcaggaaccg acacgatgaa
     6001 cgatgaattg cattcacgcg ccgagggccc ggtgcaactg gagagcaccc gcttcgacac
     6061 cttggcggtg cgcgcgggcc agacccgcac cgccgaaggc gagcatggtg agccgatctt
     6121 caccacttca agctatcttt ttgaaaacgc ttcaaatgcg gcggagcgtt ttgctggcat
     6181 cacccctggc aacgtctatt cgcgctacac caatcccacg gtgcggatgt tcgaggagcg
     6241 gctggcggca ctggagggag ccgagcaggc ggtcggcacg gcctctggca tggcggccat
     6301 tctgagcctg atgatgacgc tgttgcaggc cggtgaccat gtcatctgtt cggccagcgt
     6361 gttcggcacc acggtcgggc tgttcaaccg ctatctgcgc aagttcggcg tcgagaccac
     6421 cttcgtgcac ctgacccacc tcgacgattg gcgcgcggcg gtgcggccca acacccggct
     6481 gctgtttctc gaaaccccct ccaacccgct ctgcgaggtg gccgatctgg ccgcgctgtc
     6541 gacgctggca cgccagatcg gcgccaagct ggtggtggac aactgcttct gctctccggc
     6601 actgcaacgg ccgctcgaac tgggcgccga ttacgtggtg cattcggcca ccaaatatct
     6661 ggatggtcag gggcgttgcg tgggcggcgt ggtggccggt cctgcctcgg tgatgagcga
     6721 ggtggtggcc tttctgcgca ctgccggacc gaccatgagc cccttcaatg cctgggtctt
     6781 tctcaagggt ctggagacac tgcggttgcg catggatgcc cacagtgcca acgcgctgac
     6841 gctggcgcgc tggctgcaaa gccagcccgg catcgagcag gtctatcact gtggtctgcc
     6901 gacccatccg cagcatcagc tggcgctgcg tcagcagcgc ggattcggcg gggtcttctc
     6961 cttttcggtc agtggtgacc gcgcggctgc gtggcgcttc atcgatgcca cccgcatctt
     7021 ctcgctcacc gccaacctcg gcgatgccaa gagcaccatc attcacccgg ccaccaccac
     7081 ccatgggcgg ctgagtgccg aggagcgcac caacgccggc attggcgaca acctgatccg
     7141 tctggcggtc ggcctggagg cggtcgagga tctgcaggac gatctggcgc ggggcctggc
     7201 cgcactctga ccaccgctcc accgcgccca ttccgctgtc ctgatccgga gtcagcccga
     7261 ccatgcagaa gttggtggaa cagatcatcg ctgaggtcgg ccgggtggtg ctgggcaagg
     7321 agcggcaggt gcgtctggca ctgggctgcc tgatcgccgg tggtcatctg ctgatcgagg
     7381 atctgcccgg catgggcaag accacgctgg cgcagacgct ggcacgggtg ctggggctgg
     7441 agttctcccg catccagttc accagtgacc tgttgccggc ggacatcctg ggcgtttcgc
     7501 tgttctcgcc gcagacgcag cagttcaact ttcgtccggg tccgctgttt gcccagctgg
     7561 ttctggccga tgagatcaac cgcgccacac ccaagaccca gagcgcgctg ctcgaagcga
     7621 tggaggagca gcaggccacc gtcgatggtg tgagtcaccc gctgccgcag ccgttcttcg
     7681 tcatcgccac ccagaatccg gtgacccagc ttggcacctt tgccctgccg gagtcacagc
     7741 tcgaccgctt cctgatgcgc atcgacatcg gctatcccca cccggcggtc gagcgccgga
     7801 tgctggccgg cgagatgccg cgccagcttc tggaacatct ggccccactc ggtgatgcga
     7861 cgaccctgtt gcgattgcgt caggcggtgc ggcagatcaa ggccagtgat gcgttgctgg
     7921 actatgtgca ggcgctgatc gccttcagcc gcaatgccgg ccatttcgtc aatggtctgt
     7981 cgccccgtgg tgcccaggcc atgatggaga gcgcccgcgc ctgggcgttg ctggccggac
     8041 gcgactatct gctgcctgaa gacattcagg ccatcctgcc gagcgtggtc gaccaccggt
     8101 tgcagaccat cgagcgcgac gccgcactgc ccagcgcgct gatggtggca gccgtcgacc
     8161 cgctggcacg gcagcgcggt ggcgcgctca ggccatagtg cgccgtgcgc tggaggcgtc
     8221 ccaggctgcc tgaccggctg cagaaaggct acgaccgctg gctggcgcgt cgctttccgc
     8281 cactgtcacg gctccgcctg ggccatcggc agctcttcat cgtgccgtcg ctctccggcg
     8341 gactcttcat gctgctgctg gctgcgatgc tgctggcggc gatcaactac cagaacagcc
     8401 tcgcctttgc gctcagtttc tggctgcttg gcctgatgcc gatcgcgctg atccatgcct
     8461 accgcaacct gctcggactg gaactgaccg ccctgggcag cacagcaggc ttcgtcggcg
     8521 gtcatggaca gctgcggctg caactgcgcg ccgaacggcc actgcggcat gccatcgaac
     8581 tgcggctggg cggcggcgag tgggtgagcg acgcggacag tgaagagcgg tcgatcaccc
     8641 tgacgctgcc gcttgcaagc cgcggttggc aggcgattcc ccggctggag gtcaggtcag
     8701 gctttccgct gggactgttc cgggtctggt cctacccctg cctcgactgg aagctgctgg
     8761 cctatccaca cccctttgcc gccgatgcca tgccgccggt cagtggcgaa tcgtcggcgg
     8821 cgttgccgca cggcgtgcaa tcaggcgatg aagattttgc cggactcgac ccatggcgcc
     8881 tgggcgatgg ctggggcagg gtggcgtgga agcagtgggc ccgccgcggc gagttgctgg
     8941 tgaagcggct ggagcagccg gagcaggggg cgcagtggct cgatttcgac cactgccccg
     9001 atcggtcaat cgaagcgcgg ctgtcgcggc tggccggctg ggtgc
//
LOCUS       DAORCQ010000094         8993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5407_length_8993_cov_6.367532, whole genome shotgun sequence.
ACCESSION   DAORCQ010000094 DAORCQ010000000
VERSION     DAORCQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_8993_cov_6.367532"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKI23_09410"
     CDS             <1..635
                     /locus_tag="PKI23_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN37194.1"
                     /translation="IYEYYGGTEGGACNATPQEWLQRPGTCGKPLAGVRILCEQGEIL
                     PAGEIGTVYLPAPAEGRFQYFKDPQKTEKAYGGGVSHFTLGDMGYLDQEGYLYLCDRR
                     TDLIISGGVNIYPAEVDAVFLTHPKVADACTIGIPDPEWGQQVMTVIEPKTGVEPTDE
                     LAEALLGYCRDQLARFKCPRRIEFMASLPRSEAGKILRRKVRDHYVEESA"
     gene            723..1763
                     /locus_tag="PKI23_09415"
     CDS             723..1763
                     /locus_tag="PKI23_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37195.1"
                     /translation="MSQRKAVALISGGLDSMLAARVVQSQGIHVEGINFFTGFCVEGH
                     THAIRRQPGGKPKRNNALWVAEQLGIKLHIIDVSTPYKQVMLNPRHGYGQHLNPCLDC
                     KVFMVGEARRWMEEHGFDFIITGEVIGQRPMSQRKATLPIVARESGAEDRLLRPLCAH
                     NLLPTLPEREGWVDRAQLHGFHGRNRKPQIALAEQLGLTEYAQPAGGCCFLTDPNYSS
                     KLADLWQHRGVRDYELDDIMLLKVGRHIRPRPHFKLIISREEGENNFLEGYRKEFTHL
                     RPLSHPGPLCLIDGSTDDADLLLAARIVARFSQGRMAEQVELELVQPDHPPRRLSVAP
                     LPPTELPESWYL"
     gene            1760..1984
                     /locus_tag="PKI23_09420"
     CDS             1760..1984
                     /locus_tag="PKI23_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNN37196.1"
                     /translation="MKHQLDARRLLCPLPVIRTQARVATLQPGDLLEVIATDPGVRHD
                     LPAWCRVHGHQLIEIVEQEREIHVTLEVGG"
     gene            2378..3784
                     /gene="glnA"
                     /locus_tag="PKI23_09425"
     CDS             2378..3784
                     /gene="glnA"
                     /locus_tag="PKI23_09425"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HNN37197.1"
                     /translation="MPSRVYKLIKEREVKWVDLRFTDTMGKEQHVSIPAKTVDDSFFT
                     DGKMFDGSSIAGWKGIHQSDMILMPDPSTAVFDPFTDEVTLIIRCDVIEPTTMQGYDR
                     DPRSIARRAEEYLKSTGLGDTAFFGPEPEFFVFDDVKWKSDISGSMYSIQANEAAWSS
                     HLEVEGGNIGHRPGIKGGYFPVPPVDSSQDLRSAMCAAMESMGLEIEVHHHEVATAGQ
                     NEIGVKFNTLVKKADEVQILKYCVHNVAHSYGKTATFMPKPLVGDNGSGMHCHQSFWK
                     DGVNQFSGDKYAGLSETALYYIGGIIKHARALNAFTNASTNSYKRLVPGFEAPVMLAY
                     SACNRSASIRIPYVSSPKAVRIEVRFPDPTSNPYLGFAAMLMAGIDGVKNKIHPGDAL
                     DKDLYDLPPEEAKAIPTVAHTLEMALQALDKDRAFLTAGGVFSDDMIDAYIELKMAEV
                     IRVAQTTHPLEFAMYYSL"
     gene            3959..4537
                     /locus_tag="PKI23_09430"
     CDS             3959..4537
                     /locus_tag="PKI23_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNN37198.1"
                     /translation="MSIRQPFSAVSWSVLILWSLLQPSLVWAAEEIYRSADETGTPSF
                     TDRPAGDARKVELPAPNIDSAGVPQGEFYTPPSAAVPAAAYESVRIVSPQAEATLRSN
                     SGDLEVRVEVQPALQPQHWLLLRMDGQEMGELQKGGGFQLSNIDRGSHQLLVEVVDQD
                     RAVIAQSSPVTIHLHRHSILFPPPAGSKPAPK"
     gene            4640..5710
                     /gene="glnL"
                     /locus_tag="PKI23_09435"
     CDS             4640..5710
                     /gene="glnL"
                     /locus_tag="PKI23_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNN37199.1"
                     /translation="MYPDSLYRQILDHLNSAVILLDGDFCLRYMNPAAEALFEMSGLR
                     LIGSPVQELFLEEVETDAQTLFDLARPYTKRKATLRLTHGKEIVVDYSVSPMQGADAM
                     LLIEFQPIDRLLRIAREEAGIATSRANRVLVRGLAHEIKNPLGGLRGAAQLLARELTD
                     MRLHEYTDVIIEEADRLRNLVDRMLGPHRPPDLKPINVHEVVERVARLIQAEAGDAIM
                     LVRDYDPSIPEIQADREQLIQALLNIVRNALEAITSSPAKRPGQIILRTRTVRQHTIG
                     LVHHRLLCRIEVIDNGPGIPAEIMQNIFFPMVSGRAEGSGLGLSISQSIVHQHHGSLE
                     CDSEPGQTRFTLLIPIQDARGG"
     gene            5715..7157
                     /gene="ntrC"
                     /locus_tag="PKI23_09440"
     CDS             5715..7157
                     /gene="ntrC"
                     /locus_tag="PKI23_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004882730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNN37200.1"
                     /translation="MEQRQQVWIVDDDRSIRWVLERALEQAGVATTSFDSGDAALRRL
                     KREHPSVLISDIRMPGSDGFALLAAVQQEHPKLPVIIMTAHSDLESAVASYQGGAFEY
                     LPKPFDVDDAVSVVNRALAYAQEQEAETATEAEIAPARKTEIIGEAPAMQEVFRAIGR
                     LSHSNITVLINGESGTGKELVAQALHNHSPRSKGPFIALNMAAIPRELMESELFGHEK
                     GAFTGAAAQRKGRFEQADGGTLFLDEIGDMPADTQTRLLRVLADSEFYRVGGHTPVKV
                     DVRIIAATHQNLEQLVKSGQFREDLFHRLNVIRIHLPRLRERAEDIPRLARHFLARAA
                     EELNVEPKVLRKETVDYLCTLAWPGNVRQLENTCRWITVMASGREVHIRDLPPELHQQ
                     PESPIQPCNWQQALSQWADQELARGNKGLLDYALPEFERIMIQTAIKQTAGRRRDAAL
                     LLGWGRNTLTRKIKELGLQIQHDEDEEESG"
     gene            7231..7698
                     /locus_tag="PKI23_09445"
     CDS             7231..7698
                     /locus_tag="PKI23_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNN37201.1"
                     /translation="MLHLLLHQPQIAPNTGNLIRLCANTGCQLHLIEPLGFTLDDKRL
                     RRAGLDYHEWARVERHRDFDEFLQRLASPPRIYAFSTRAQRCYSTVSYQPDDVLLFGS
                     ETQGLPDELLARLPTQQQLRLPMQPGSRSLNLANAAAIALYEAWRQLGFNGGV"
     gene            complement(7732..8175)
                     /gene="secB"
                     /locus_tag="PKI23_09450"
     CDS             complement(7732..8175)
                     /gene="secB"
                     /locus_tag="PKI23_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNN37202.1"
                     /translation="MSDQQTPPSQFSIQRIYVKDLSFESPRAPEVFRGGWQPEIRLEM
                     NTQHRAIEGDLHEVILAVTITAEMEAKKVFLAEIQQAGLFQVQFPSEQETARILGSFC
                     PNVLFPYAREALDSLVVRGGFPPLMLAPVNFDALYQQALQQRSAN"
     gene            complement(8239..8505)
                     /gene="grxC"
                     /locus_tag="PKI23_09455"
     CDS             complement(8239..8505)
                     /gene="grxC"
                     /locus_tag="PKI23_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNN37203.1"
                     /translation="MAATVTLYTSAFCGYCHRAKALLAQKGVDYSELSVDGQPQRRRE
                     MERLSGRSTVPQIWIGTTHVGGCDELYALERAGRLDALLARAGE"
     gene            complement(8527..>8993)
                     /locus_tag="PKI23_09460"
     CDS             complement(8527..>8993)
                     /locus_tag="PKI23_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNN37204.1"
                     /translation="TPTGCRCAALLEVGMVMARFFEFAANHWQLVSLFVALGGLFFWM
                     EHRRRGGSISCQQAVDLINKQEGVVLDVRDSGEYKAGHIVNALHIPHAKVAEQIAQLD
                     KYRARPIIVVCGMGQQAGTVCETLKKSGFEQVHRLGGGMGSWQGEKLPVVRK"
BASE COUNT         1710 a         3002 c         2740 g         1541 t
ORIGIN      
        1 tcatctatga atactacggc ggcaccgagg gcggcgcctg caacgccaca ccgcaggagt
       61 ggctgcagcg gcccggcacc tgcggcaagc cactcgctgg ggtgcgcatc ctctgcgaac
      121 agggtgaaat cctgccagcc ggcgagatcg gcaccgtcta tctgccggca ccggccgagg
      181 ggcgcttcca gtacttcaag gatccgcaga agaccgagaa ggcctatggc ggcggtgtca
      241 gccacttcac cctcggcgac atgggctatc tcgaccagga gggctacctc tatctctgcg
      301 accgccgcac cgatctgatc atttccggtg gcgtcaacat ctatccggcc gaggtcgatg
      361 cggtttttct gacccatccc aaggtggcgg acgcctgcac catcggcatt cccgatccgg
      421 agtggggaca gcaggtgatg acggtgatcg aaccgaagac cggcgtcgaa ccgaccgatg
      481 agctggccga agcgctgctc ggctactgcc gcgaccagct cgcccgcttc aagtgtccgc
      541 gccgcatcga attcatggcc agtctgccgc gctccgaagc gggcaagatc ctgcgccgca
      601 aggtgcgcga ccactatgtg gaggagagcg catagccgca cgacccccgg gtttcatttc
      661 atccgccact ggcgtttaat ggcgcccccc ggcatccggg gtgccacagg ggggatagaa
      721 ccatgagcca acgcaaggcg gtcgcgctga tctccggcgg cctcgactcg atgctggctg
      781 cccgcgtggt gcagagccag ggcatccatg tcgagggcat caacttcttc accggcttct
      841 gtgtcgaggg ccacacccac gccatccgca ggcagccggg cggcaagccc aagcgcaaca
      901 acgcgctctg ggtcgccgag cagctcggca tcaagctgca catcatcgat gtctccaccc
      961 cctacaagca ggtgatgctc aaccctcgcc atggctatgg ccagcacctc aacccctgcc
     1021 tcgactgcaa ggtgttcatg gtcggcgagg cccggcgctg gatggaagag catggcttcg
     1081 acttcatcat caccggcgaa gtgatcggcc agcggccgat gtcgcagcgc aaggcgacgc
     1141 tgccgatcgt cgcccgtgaa tcgggcgccg aagatcggct gttgcggccg ctctgcgcgc
     1201 acaatctgct gccaacgctc ccggagcgcg aaggctgggt cgaccgcgcg cagctgcatg
     1261 gttttcatgg ccgcaaccgc aagccacaga tcgcgctggc cgagcagttg ggcctcaccg
     1321 aatatgccca accagccggt ggctgctgct ttttgaccga ccccaactac agcagcaagc
     1381 tggccgatct gtggcagcac cgtggcgtgc gtgactacga actggatgac atcatgctgc
     1441 tgaaagtggg gcgccacatc cggccgcggc cccacttcaa gctgatcatc agccgcgaag
     1501 agggtgagaa caactttctc gaaggctacc gcaaggagtt cacccatctg cgcccgctga
     1561 gccatcccgg accgctctgc ctgatcgacg gcagcaccga tgacgccgac ctgctgctgg
     1621 ccgcccgcat cgtcgcccgc ttcagccagg gccgcatggc cgaacaggtc gaactggagc
     1681 tggtgcagcc cgaccatccg ccccggcggc tcagcgtcgc gcccctgcca cccaccgaac
     1741 tgcccgagag ctggtatctg tgaagcacca gctcgatgcc cgccgcctgc tctgcccgct
     1801 gccggtgatc cgcacccagg cgcgggtggc cacgctgcaa ccgggcgacc tgctcgaggt
     1861 gatcgccacc gatccgggtg tcaggcatga cctgccggcc tggtgccggg tgcatggtca
     1921 ccagctgatc gagatcgtcg aacaggagcg ggagatccac gtcacccttg aagtcggggg
     1981 ttaatgcggg cgcccagacg cattggctgg gcttgcgcca gcaacgaacg cacaataaaa
     2041 gtgcaaaaaa ctggcgcaag ttccattgtg gtgcacatat tgtccgcttt tggccgggtg
     2101 ctgatccgat ttggctcatc cttgtcgccg agaatcggag cacggttgca tttgaccatc
     2161 cattgcggct aaatcgccgg ctttgacggc tcctgttttt gggcactgcg cgctggatcc
     2221 gcacaaatgg atccccaaac gctgctggca tgatctctgc tatgctcttc gtgcagtgac
     2281 catccgcatc ccgatggcca ttccctgtca gaccgtcttt cgattcgacc gataacaccc
     2341 caatggcgcc gcgcgccatg cattccctgg aggagccatg ccaagcagag tctataaatt
     2401 gatcaaagag cgtgaggtca agtgggtcga cctgcgcttt accgatacca tgggcaagga
     2461 gcagcatgtc tccatcccgg ccaagacggt ggatgacagc ttcttcaccg atggcaagat
     2521 gttcgatggc tcttcgattg ccggctggaa aggcattcac cagtcggaca tgatcctgat
     2581 gcccgacccc agcaccgcgg tgttcgaccc cttcaccgac gaggtcacgc tgatcatccg
     2641 ctgcgacgtc atcgagccga ccacgatgca gggctacgac cgtgatccac gctcgatcgc
     2701 ccgccgtgcc gaggagtatc tgaaatcgac cggactcggc gacaccgcgt tctttggccc
     2761 ggagcccgaa ttcttcgtct tcgacgacgt gaagtggaaa tccgacatca gtggctcgat
     2821 gtacagcatt caggccaacg aagcggcctg gtcgagccac ctggaggtcg agggcggcaa
     2881 catcggccac cgtcccggca tcaagggcgg ctacttcccg gtgccgccgg tcgacagctc
     2941 gcaggatctc cgcagcgcca tgtgcgccgc catggagtcg atggggctcg aaatcgaggt
     3001 gcaccaccat gaagtggcca ccgctggcca gaacgagatc ggcgtcaagt tcaacacgct
     3061 ggtgaagaaa gcggacgaag tacagattct gaagtactgc gtccacaacg tggcccactc
     3121 ctacggcaag accgccacct tcatgcccaa gccgctggtc ggcgacaacg gcagcggcat
     3181 gcactgccac caatccttct ggaaggatgg cgtcaaccag ttctccggcg acaagtatgc
     3241 cggcctgtcg gagaccgcgc tctactacat cggcggcatc atcaagcatg cccgcgcgct
     3301 caacgccttc accaatgcct cgaccaactc ctacaagcgg ctggtgcccg ggttcgaggc
     3361 gccggtcatg ctggcctatt cggcctgcaa ccgctcggcg tcgatccgca tcccctatgt
     3421 gagcagcccc aaggcggttc gcatcgaggt gcgcttcccc gaccccacct ccaaccccta
     3481 cctcggcttc gcggcgatgc tgatggccgg catcgacggc gtcaagaaca agatccaccc
     3541 gggcgatgcg ctggacaagg atctctacga cctgccgccc gaagaggcca aggcgatccc
     3601 gacggtcgcc catacgctgg agatggcgct gcaggccctc gacaaggacc gcgccttcct
     3661 caccgctggc ggggtcttct ccgatgacat gatcgatgcc tacatcgagc tgaagatggc
     3721 cgaggtgatc cgcgtcgccc agaccaccca tccgctcgag tttgcgatgt actacagcct
     3781 ctgaggtgac atcgccggcc gtccacggga cggctgtcgg cccgtcgaaa gccccggtcc
     3841 agtgccgggg ttttctgtct gggcacagcg gcgcagccgg atggtcacgg gcaatgctct
     3901 tgatgaaaac gtcgggctgc gcaacactgg accgaccacg attcaacagg tgtgatgcat
     3961 gtcgatccgt cagccattca gcgcagtgtc gtggtcggtt ctgatcctct ggtcactgtt
     4021 gcagccatcg ctggtttggg ccgccgagga gatttatcgt tctgccgatg agacaggcac
     4081 tccgagcttc accgaccgcc cggcaggtga tgcgcgcaag gtggagctgc ccgcgcccaa
     4141 catcgacagc gccggagtgc cgcagggcga gttttacacc ccgccctccg ctgccgttcc
     4201 ggccgcggct tacgagtcgg tgcgcatcgt ttcgccgcag gcggaggcca cgctgcgcag
     4261 caacagtggc gatctggagg tgcgggtgga ggtgcaacca gccctgcaac cccagcactg
     4321 gctgctgctg cggatggatg gccaggagat gggggagctg cagaagggtg gcggctttca
     4381 gctcagcaac attgaccgcg gcagccacca actgctggtg gaggtggtcg atcaggaccg
     4441 tgcggtgatc gcacagagct cgcccgtgac catccatctg caccgccatt caatcctctt
     4501 cccgccgcct gccggcagca aaccagcccc gaagtagcat cgctttcaac cgtgcgcagc
     4561 gcggggttcc acgctctcca cgagcgaaat ggttggcccc gcttttgctt tgcttgattg
     4621 catgcaacat ggcgctcaca tgtatccaga cagtctctac cgacagattc tcgaccatct
     4681 caacagtgcg gtgattctgc tcgatggtga tttctgcctg cgctacatga atccggccgc
     4741 cgaagcgctg ttcgagatga gtggtctgcg gctgatcggc agcccggtgc aggagctgtt
     4801 cctcgaggag gtcgagacgg atgcgcagac actgttcgat ctcgcccggc cctacaccaa
     4861 gcgcaaggcg accctgcggc tcactcatgg caaagagatc gtggtggact attcggtctc
     4921 accgatgcag ggggccgatg ccatgctgct gatcgagttc cagccgatcg accggctgct
     4981 gcggattgcc cgcgaggagg ccggcattgc caccagccgg gccaatcggg tgctggtgcg
     5041 cgggctggcg cacgagatca agaatccgct gggcggactg cgtggcgccg cgcaactgct
     5101 ggcgcgcgag ttgaccgaca tgcggctgca tgagtacacc gacgtgatca tcgaagaggc
     5161 cgatcggctg cgcaacctgg tcgaccgcat gctgggacca caccgtccac ccgacctcaa
     5221 accgatcaac gtgcacgagg tggtggagcg ggtggcgcgg ctgatccagg ccgaggccgg
     5281 cgatgcgatc atgctggtgc gcgactatga cccgagcatc ccggagattc aggccgaccg
     5341 cgagcagttg attcaggcac tgctgaacat cgtccgcaat gcgttggagg cgatcaccag
     5401 cagtccggcg aaacggccgg ggcagatcat tctgcgcacc agaacggtgc gtcagcacac
     5461 catcggcctg gtgcatcatc gtctgctctg ccgcatcgag gtgatcgaca acggcccggg
     5521 cattccggcc gagatcatgc agaacatttt ctttccgatg gtcagcggcc gggccgaggg
     5581 gagcggcctt ggactttcga tttcgcaatc gatcgttcat caacaccatg gcagcctgga
     5641 gtgtgacagc gaacctggtc agacccgctt cacgctgctg attccaattc aagatgcgcg
     5701 cggtggatag attcatggaa caacgtcaac aggtctggat cgtcgatgac gaccgctcca
     5761 ttcgctgggt gctggaacgg gcactggagc aggcgggggt ggcgaccacc agtttcgaca
     5821 gtggcgatgc ggccctgcgg cggctgaagc gcgagcatcc gagcgtgctg atcagcgaca
     5881 ttcggatgcc gggcagcgac ggctttgcgc tgctggcagc cgtgcagcag gagcacccca
     5941 agctgccggt catcatcatg accgcccact ccgacctcga aagcgcggtg gcctcctacc
     6001 agggcggtgc cttcgagtac ctgcccaagc ccttcgatgt cgacgatgcc gtctcggtgg
     6061 tgaaccgggc gctggcctat gcccaggagc aggaagcaga aacagccacc gaagccgaaa
     6121 tcgccccggc acgcaagacc gagatcatcg gcgaggcgcc agcgatgcag gaggtgttcc
     6181 gcgccatcgg tcggctgtcg cactccaaca tcaccgtatt gatcaacggc gaatccggta
     6241 ccggcaagga gctggtcgcc caggcactgc acaaccacag cccccgctcc aaggggccct
     6301 tcatcgccct caacatggcg gcgattccac gcgagctgat ggaatccgaa ctcttcggcc
     6361 acgaaaaggg cgccttcacc ggcgcggccg cccagcgcaa gggacgcttc gaacaggccg
     6421 atggcggcac actctttctc gacgagatcg gcgacatgcc ggccgacacc cagacccggc
     6481 tgctgcgggt gctggccgac agcgagttct accgggtggg cggccacacc ccggtcaagg
     6541 tcgatgtgcg catcattgcg gccacccacc agaacctcga acaactggtc aagagcgggc
     6601 agttccgcga agatctgttc caccggctca atgtcatccg catccacctg ccgcggctgc
     6661 gcgaacgggc cgaggacatt ccccgtctgg cccgccactt tctggcccgt gcggccgaag
     6721 agctcaatgt cgaacccaag gtgctgcgca aggagacggt cgactacctc tgcaccctcg
     6781 cctggcccgg caatgtccgg cagctggaga acacctgccg ctggatcacc gtgatggcca
     6841 gtggccgtga ggtgcatatc cgcgacctgc cgcccgagct gcaccaacag cccgagagcc
     6901 cgatccagcc ctgcaactgg caacaggcgc tgagccagtg ggccgatcag gagctggcgc
     6961 gcggcaacaa agggctgctc gactacgccc tgccggagtt tgaacgcatc atgatccaga
     7021 ccgccatcaa gcagactgcg ggccgccgcc gcgatgccgc cctgctgctc ggctggggcc
     7081 gcaacacgct gacccgcaag atcaaggagc ttggcctgca gatccagcac gacgaggatg
     7141 aagaggagtc cggctgaggt gatgcgtcgc agggccgccc gcttcggcta gaatgcgcct
     7201 gccttcgatc gaccttctga ctgcactgcc atgctgcatc tgctcctgca tcagccacag
     7261 attgccccca acaccggcaa cctgatccgt ctgtgcgcca acaccggttg ccaactgcac
     7321 ctgatcgaac cgctcggctt cacgctggat gacaaacggc tgcgccgcgc cgggctcgac
     7381 taccacgaat gggcccgcgt agagcgtcac cgcgacttcg atgagttcct gcagcggctg
     7441 gcatcgccgc cgcgcatcta tgccttctcg acccgcgccc agcgctgcta cagcacggtg
     7501 agctaccagc cggacgatgt gctgctgttc ggttcggaaa cccagggcct gccggacgag
     7561 ctgctggcac ggctgccaac acagcagcag ttgcggctgc cgatgcaacc gggcagccgc
     7621 agcctcaacc tggccaacgc cgccgccatc gccctctatg aggcgtggcg gcagcttggc
     7681 ttcaacggcg gggtctgatc tgacgacagc cggggagaga gcccccctga ctcagttggc
     7741 cgagcgctgt tgcagcgcct gctggtagag cgcgtcgaag ttgaccggtg ccagcatcag
     7801 cggtgggaag ccgccacgca ccaccagact gtcgagggcc tcgcgcgcat aggggaacag
     7861 aacgttcggg cagaagctgc ccaggatgcg tgcggtctcc tgctcgctgg ggaactgcac
     7921 ctgaaacagg ccagcctgct ggatctcggc cagaaagacc ttcttcgcct ccatctcggc
     7981 ggtgatggtg accgccagaa tcacctcgtg cagatcgccc tcgatggcac gatgctgggt
     8041 gttcatctcc agcctgatct cgggctgcca gcccccgcga aacacctcgg gcgcgcgcgg
     8101 cgattcgaaa gagagatcct tcacatagat gcgctggatc gagaactggc tgggaggggt
     8161 ctgttgatcg ctcatggaaa agagaactcc tgttgctccg ctggcgcggc agatgggttt
     8221 atcaaatgga gggattgctc attcgccggc acgggccagc agcgcatcga gccggccggc
     8281 gcgctccagc gcgtacaact catcgcaccc accgacatgg gtggtgccaa tccagatctg
     8341 cggcaccgtc gagcgaccac tcaaccgctc catctcgcgc cgccgctgcg gctggccatc
     8401 gaccgacagc tcactgtagt cgacgccttt ctgcgccagc agcgccttgg cgcggtggca
     8461 atagccgcaa aatgccgaag tgtagagtgt gactgtcgct gccatcggtc cgcctgcctg
     8521 aaactgctac ttcctgacca ccggcagctt ttcgccctgc caactgccca tgccgccacc
     8581 caggcgatgg acctgctcaa aaccggactt cttcaacgtc tcgcagaccg taccggcctg
     8641 ctggcccatg ccgcacacca caatgatagg ccgtgcgcgg tacttatcca gctgcgcgat
     8701 ctgctcggcc accttggcat ggggaatgtg cagggcattg acgatgtggc cggccttgta
     8761 ctcgccgctg tcgcgcacat cgagcacgac cccctcctgc ttgttgatca gatcgaccgc
     8821 ctgctggcaa ctgatcgaac cgccccgccg gcgatgctcc atccagaaga acagcccgcc
     8881 aagcgccacg aacagcgaca ccagttgcca gtggtttgcc gcaaactcaa aaaaccgtgc
     8941 catgaccatc ccgacttcaa ggagcgcggc gcagcgacac cccgtgggcg tgc
//
LOCUS       DAORCQ010000095         8669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5681_length_8669_cov_5.733457, whole genome shotgun sequence.
ACCESSION   DAORCQ010000095 DAORCQ010000000
VERSION     DAORCQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5681_length_8669_cov_5.733457"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..840)
                     /gene="eda"
                     /locus_tag="PKI23_09465"
     CDS             complement(214..840)
                     /gene="eda"
                     /locus_tag="PKI23_09465"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNN37205.1"
                     /translation="MEIEAILKLAPVMPVITLERIEQALPLARALLAGGVRALEVTLR
                     TPVALRAIELLAGHDEGWVVGAGTVTTAAEYQSVVEAGARFAVSPGLTPALAAAGQQG
                     PIPLLPGVMTPSEILNARDQGFGFLKLFPAEQAGGIPFLDAIHGPLPDIRFCPTGGVG
                     PANLTDYLKLPNVLCVGGSWVTPRRLLRDGHWSEIEALARAAVTVGKA"
     gene            complement(889..1719)
                     /locus_tag="PKI23_09470"
     CDS             complement(889..1719)
                     /locus_tag="PKI23_09470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN37206.1"
                     /translation="MAAFTVLLTGATSGIGLETARALATPERRLILACRNRSKAETLR
                     QQLLSAQPACQIDLVDLDLASLASVDRAVTEIFQCCSRLDVLINNAGVMPAQFTRSVD
                     GHELNFAVNHLAPFSLTQQLLPLLRASGRARVVNVSSMMHRFGRPDFDDWPHPARYRQ
                     VRAYGSSKLGLLLCTREFARRHQRDGITANALHPGGVDTAITDYPGWMRLFLRSARKG
                     AETSIYLATAAEVASVTGGYFIDCQPKPGTALSNRADLAAQLWQRSEAWLAEWRATAA
                     "
     gene            complement(1826..2218)
                     /locus_tag="PKI23_09475"
     CDS             complement(1826..2218)
                     /locus_tag="PKI23_09475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNN37207.1"
                     /translation="MTEDELPPQPGGELALKTVALPRDTNAHGDISAGWLVSQMDLAA
                     EITAANLAKGRVVTVAIEELAFMRPIAIGASVNCHTEILEIGRSSMRILVEVWTCLPA
                     DPEPYKITEGEFVLVALDDEGRARRVVV"
     gene            2363..3130
                     /gene="yaaA"
                     /locus_tag="PKI23_09480"
     CDS             2363..3130
                     /gene="yaaA"
                     /locus_tag="PKI23_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004406753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNN37208.1"
                     /translation="MLTILSPAKNLDFETIPCTRHASQPEFVEASSELVGLLRPMTAQ
                     QLGELMQISPRLAELNQRRYQQWQPRFEAGPCKQALLAFAGEVYVGLEAASLSEADLL
                     HAQQHLRILSGLYGLLRPLDLIHPYRLEMGTPLANPHGRDLYHFWGDRITDALNRQLG
                     GSGVLINLASQEYFKAVQPKRLAGRLITPQFKDYKGGTYRTIGLLAKRARGAMARAII
                     LNRWERPDALKTFDWQGYRFNAELSSATEWVFSRRQA"
     gene            3177..4982
                     /locus_tag="PKI23_09485"
     CDS             3177..4982
                     /locus_tag="PKI23_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNN37209.1"
                     /translation="MATWDCDPVTADEADTLDALFRARVRRTPTAVAYRAYDRQRQGW
                     RDYRWREIGQAVQQWQQALRREPLQAGDRVAIALPNSPQWVIFDQAALGLGLVVVPLY
                     VDDRPNNTAYILEDCGARLLLLVDETHWLTLAGALAGIPTLERILLLEGDAACRFDDP
                     RLRSVAGWLPTPGAEQPPLPPVEPRQLATLIYTSGTTGRPKGVMLSHANILANVHGIL
                     SRIEVYRQDLLLSFLPLAHALERTAGYYVPMAAGATVVHGRGIQLLARDLSRQRPTAL
                     ICVPRVYETLHGRLSRQLMQRGALLAWLFRWAVAVGWHRFQYRQRRAGWHPRLLLWPL
                     LQPLVARPLQQRLGGRLRLAVSGGAALQPEVARRFIGPGIPIVQGYGLTEASPVVSCN
                     TLHDNDPASVGRPLPGVALHIGEQGEVLVRGPNVMLGYWHGRADDPAIDAQGWLHTAD
                     QGWLEDGRLHLRGRLKETIVLCNGEKIPPDDLEMAIRLDPLFSQVMLAGSGQPRLVAL
                     VVLEPELWREQAIQWGVEPDDAALNDPVVKQLLLARMNGLLKEFPAYATLGRAHFSLT
                     PWRVADGLLTPTLKLKRSQLLERFAGQLRQLQQGS"
     gene            complement(5010..6239)
                     /gene="serA"
                     /locus_tag="PKI23_09490"
     CDS             complement(5010..6239)
                     /gene="serA"
                     /locus_tag="PKI23_09490"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNN37210.1"
                     /translation="MTRLSLDKDRIKILLLEGIHPSAVQTLRQAGYSNIEALPTALEG
                     EELKARIRDVHFIGIRSRTRLNAEVLEAAQRLVAIGCFCIGTNQVDLNQALLQAIPVF
                     NAPFSNTRSVAELVLAEAILLLRGIPEKNALAHRGGWLKSASNAYEIRGKQLGIIGYG
                     NIGTQLGVMAESLGMKVRFYDVVNKLSIGNAVQLHDLHELLATSDVVTLHVPETAATR
                     NMIGSAELAAMKPTGVLINASRGTVVDIDALAQALADKRLLGAAIDVFPVEPKGNDTP
                     FVSPLQAFDNVILTPHIGGSTIEAQANIGVEVAEKLIKYSDNGTTTSSVNFPEVALPA
                     HPGKHRLLHIHRNEPGVMSAINRVFSDSGINISAQYLQTNDRTGYVVIDVDAEYSELA
                     LKRLQEIPATLRARALF"
     gene            6423..6905
                     /locus_tag="PKI23_09495"
     CDS             6423..6905
                     /locus_tag="PKI23_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNN37211.1"
                     /translation="MNSTIVERATQLGLLLKQRGLRVATAESCTGGLVAAAITAVAGS
                     SAWFEYGWVVYSNEAKRTLLGVAADTLQRFGAVSHETVRELARNALLRADADLAVAVS
                     GIAGPGGATAHKPVGTVCFGWAGRDGRLETESLHFTGDRAGVREQAALHALAGLMARA
                     "
     gene            6974..7942
                     /gene="recA"
                     /locus_tag="PKI23_09500"
                     /pseudo
     CDS             6974..7942
                     /gene="recA"
                     /locus_tag="PKI23_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702372.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            8117..>8669
                     /locus_tag="PKI23_09505"
     CDS             8117..>8669
                     /locus_tag="PKI23_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130353.1"
                     /note="involved in the post-transcriptional processing of
                     the daa operon mRNA, which encodes proteins involved in
                     fimbrial biogenesis of an enteropathogenic E. coli strain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNN37212.1"
                     /translation="MLGRIASRLAAAKPADRLLSEFRQQLDASSSWVEARLQSIPTID
                     YPLELPVVAARERLLDAIERHQVVVLAGETGSGKTTQLPKLCLELGCGSRGMIGHTQP
                     RRLAARAVAQRLAEELRVPLGQQVGAQVRFSDQSGPQTLVKVMTDGILLAEIGHDPLL
                     TAYDTLIIDEAHERSLNIDFLLGY"
BASE COUNT         1405 a         2771 c         3025 g         1468 t
ORIGIN      
        1 gccgcgaccc ggcggctcga atgacacctt gtcaccgatg ctgaatttga gcatttcagt
       61 gtgctggtgc atcgattcaa gaaacttgag cctttgcacg atgcgctgat tcagttcgat
      121 cagctctgcc tcggtcattg tgtcgatgtc gatcttcatg aattgttcca gatcattaag
      181 tggctgaatc gtgtgtcagt gcgcatgcgc cggtcatgcc tttccgaccg tcaccgccgc
      241 gcgggccagc gcctcgatct cgctccagtg gccgtcgcgc agcagtcggc gcggtgtcac
      301 ccaggagccg ccgacgcaga gcacattggg cagtttcagg tagtcggtga ggttggccgg
      361 gccgacgccg ccggtggggc agaagcggat gtcgggcagt gggccgtgga tggcgtcgag
      421 aaaggggatg ccgccggcct gttcggccgg aaagagtttg agaaaaccaa aaccctggtc
      481 gcgtgcattg aggatttcgc tgggggtcat gacgccgggc agcagcggaa tcggtccctg
      541 ctggccggcc gccgccagcg cgggcgtcag gccggggctg acggcgaagc gggcgccggc
      601 ctcgacgaca ctctggtact cagcggcagt ggtgacggtg ccggcaccga ccacccagcc
      661 ttcgtcatgg ccggccagca gttcgatggc ccgcagcgcc accggggtgc ggagcgtcac
      721 ctccagtgcg cgcacgccgc cggccagcag cgcgcgggcc agtggcaggg cctgttcgat
      781 tcgctccagc gtgatcaccg gcatcaccgg cgccagcttc agtatcgctt cgatctccat
      841 cgtgcagccc tcggcagttt cgtggcaaca gcctggtggc agcaatcctt atgccgctgt
      901 cgcgcgccac tcggcgagcc aggcttcgct gcgttgccac agttgcgcgg cgaggtcggc
      961 acggttgctg agcgcggtgc cgggcttggg ctggcagtcg atgaagtaac cgccggtcac
     1021 gctggccact tcggcggcgg tggcgaggta gatcgaggtt tctgccccct tgcgcgcgga
     1081 gcgcaggaac agccgcatcc agcccggata gtcggtgatg gcggtgtcga cgccgcccgg
     1141 gtgcagggcg ttggcggtga tgccgtcgcg ctggtggcgg cgggcaaact cacgggtgca
     1201 gagcagcagt ccgagcttgg agctgccata ggcgcgcacc tgtcggtaac gggccggatg
     1261 gggccagtcg tcgaaatcgg ggcgaccaaa gcggtgcatc atcgagctga cattgaccac
     1321 ccgcgccctg ccgctggcgc gcaacagtgg cagcagttgc tgggtcagcg agaacggcgc
     1381 aaggtggttg acggcgaagt tgagctcgtg gccatcgacc gagcgggtga actgggcagg
     1441 catgacgccg gcgttgttga tcagcacgtc aaggcgtgag cagcactgaa agatctcggt
     1501 cacggcgcgg tcgaccgagg ccagactggc caggtcgaga tccaccagat cgatctggca
     1561 ggcgggctgg gcgctcagca gttgctggcg gagggtctcg gccttggatc ggttgcggca
     1621 ggccagaatc agccgtcgct cgggtgtggc cagggcgcgg gcggtctcca gtccgatgcc
     1681 gctggtggcg ccggtgagca gaacggtgaa agcggccatg caatccctcc tggatcagat
     1741 tggtgcactt ttttgatggt gaccgatcgc tggcgtggcg tccagcaccc tgccggcatg
     1801 ccggtccggg tggcgaaaga gtcggctaaa ccaccacccg gcgcgcgcgg ccctcgtcgt
     1861 cgagggccac caggacgaac tccccttcgg tgatcttgta gggttccggg tcggcgggca
     1921 ggcaggtcca gacctccacc agaatgcgca tcgaactgcg gccgatttcg aggatctcgg
     1981 tgtgacagtt gacgctggcg ccaatcgcga tcggccgcat gaaggcgagc tcttcgatgg
     2041 cgaccgtcac cacccgtccc ttggccaggt tggcggcggt gatttcggcc gcgaggtcca
     2101 tctgcgacac cagccagccg gccgagatgt cgccatgggc attggtgtcg cgcggcaggg
     2161 caacggtctt gagtgccagt tcaccgccgg gctgcggcgg cagttcatct tcggtcatcg
     2221 gtgggaggac tgttattgtt gtgggcaggg ggcgatccgg cggcattata ggcattccag
     2281 gctgcgttgg tgacaattgc aaccgtgacg gcttttgcta ccgtgttcgg cccgtgacgc
     2341 gagccatgcc aaggtgtctg ccatgttgac cattctctcc ccggccaaga acctcgattt
     2401 cgagacgata ccctgcaccc gccatgccag tcagccggag ttcgtcgaag ccagcagcga
     2461 actggtcggg ctgctgcggc cgatgacggc gcagcagttg ggcgaattga tgcagatcag
     2521 ccccaggctg gccgagctga accagcgtcg ctatcagcag tggcagcccc gcttcgaagc
     2581 gggaccctgc aagcaggcgc tgctcgcctt tgccggcgag gtctatgtcg ggctggaggc
     2641 cgccagcctg agcgaggccg atctgctcca tgcccagcag catctgcgca tcctctccgg
     2701 cctctacggg ctgctgcgcc cgctcgatct gatccatccc tatcggctgg agatgggcac
     2761 cccgctcgcc aatccccatg gccgcgacct ctatcacttc tggggtgatc ggatcaccga
     2821 cgccctcaac cggcaactgg ggggcagtgg cgtactgatc aatctggcgt cgcaggagta
     2881 cttcaaggcg gtgcagccga agcggctcgc tggccggttg atcacgcccc agttcaagga
     2941 ttacaaaggt ggcacctacc gcaccatcgg cctgctggcc aagcgggcgc gcggtgcgat
     3001 ggcgcgcgcg atcatcctca accgctggga gaggcccgac gcgctcaaga cgttcgactg
     3061 gcagggctac cgcttcaacg ccgagctctc atccgccact gagtgggtgt tcagccgccg
     3121 gcaggcgtga gcggaacggc ccgcggatcg cacggcccac agggaggagc acgacgatgg
     3181 caacctggga ctgcgatccg gtcactgccg acgaggccga cacgctggat gcgctgtttc
     3241 gcgcgcgcgt gcggcgcacg cccacggcag tggcctaccg cgcctacgac cggcagcgtc
     3301 aaggctggcg tgattaccgt tggagagaga tcgggcaggc ggtgcaacag tggcagcagg
     3361 cgctgcggcg ggagcctctt caagcgggtg accgcgtcgc catcgccctg cccaactctc
     3421 cgcagtgggt catcttcgat caggctgcgc tggggctggg attggtggtg gtgccgctct
     3481 atgtcgacga ccgtcccaac aacaccgcct acatcctgga ggattgcggg gcgcgcctgc
     3541 ttctgctggt cgacgagacc cactggctga cgctggccgg ggcgctcgcc gggatcccga
     3601 cactggagcg catcctgctg ctggagggtg atgcggcctg caggttcgac gatcctcggc
     3661 tgcgcagcgt ggccggctgg ttgccgacgc ccggcgcaga gcagccgcca ctgccgccgg
     3721 tcgagccacg acagttggcg acgctgatct acacctccgg caccaccgga cgccccaagg
     3781 gggtgatgct gagccatgcc aacattctgg ccaatgtcca tggcattctg agccggatcg
     3841 aggtctaccg gcaggatctg ctgctctcct ttttaccgct cgcccatgcg ctggaacgga
     3901 ccgccggcta ctatgtgccg atggcggccg gcgccaccgt ggtccatggc cgtggcattc
     3961 agcttctggc gcgcgatctg tcacggcagc ggccgacggc gctgatctgc gtgccccgcg
     4021 tctacgaaac cctccatggc cgactcagcc ggcaactgat gcagcgcgga gcgctgctcg
     4081 cgtggctgtt ccgctgggcg gtcgcggtgg gctggcaccg ctttcagtac cggcagcggc
     4141 gggcaggctg gcatccccga ttgctgctct ggccgctgct gcagccgctg gtcgcccggc
     4201 cgctgcaaca gcgcctgggt ggacggctgc ggctggcggt cagtggcggc gccgcgctgc
     4261 aacccgaggt ggcgcggcgc ttcatcggcc cgggcattcc gatcgtgcag ggctatggcc
     4321 tgaccgaggc cagcccggtg gtcagttgca acaccctgca cgacaacgat cccgccagcg
     4381 tcggacggcc gctgcccggc gtggcgctgc acatcggcga gcagggtgag gtgctggtgc
     4441 gcggtcccaa tgtgatgctg ggctactggc acggccgcgc cgacgacccg gcgatcgatg
     4501 cgcagggctg gctgcacacg gcggatcagg gctggctgga ggatggacgg ctccatcttc
     4561 gcggtcggct gaaggagacc atcgtgctct gcaatggcga gaagatcccg ccggacgacc
     4621 tggagatggc gattcggctc gacccgctgt tcagtcaggt gatgctggcc ggcagcggtc
     4681 agccccggct ggtggcgctg gtggtgctgg agccggaact ctggcgggaa caggccattc
     4741 agtggggtgt ggagcccgac gatgcagcgc tgaacgatcc ggtggtgaag cagctgctgc
     4801 tggcacgcat gaatggactg ctgaaggagt ttccggccta tgccaccctc ggccgggccc
     4861 acttcagcct gaccccctgg cgggtcgccg atggcctgct gacgccgacg ctgaagctga
     4921 agcggtcgca actgctcgaa cggttcgccg gacagctgcg gcagcttcag caggggtcat
     4981 gaacggacgg gccgaccccg caccggcact cagaacagcg cgcgggcgcg cagcgtggcc
     5041 ggaatctcct gcagccgctt cagcgccagc tcgctgtatt cggcatcgac atcgatcacc
     5101 acatagccgg tgcggtcgtt ggtttgcagg tactgggcgc tgatgttgat gccgctgtcg
     5161 gaaaagaccc ggttgatcgc gctcatcacc ccgggttcgt tgcgatggat gtgcagcagc
     5221 cgatgcttgc cgggatgggc cggcagcgcc acctcgggaa agttgaccga cgaggtggtg
     5281 gtgccgttgt cgctgtattt gatcagcttt tcggccacct cgaccccgat gttggcctgc
     5341 gcctcgatgg tgctgccgcc gatgtggggg gtgagaatga cattgtcgaa cgcctgcagc
     5401 ggcgagacga acggggtgtc attgcccttg ggctcgaccg gaaagacatc gatggcggcc
     5461 cccagcagcc gcttgtcggc cagggcctgc gccagtgcgt cgatgtcgac caccgtgccg
     5521 cgcgaggcgt tgatcagcac gccggtcggc ttcatcgccg ccagctcggc gctgccgatc
     5581 atgttgcggg tcgccgcggt ttccggcaca tgcagcgtca ccacatccga tgtcgccagc
     5641 agttcatgca ggtcgtgcag ctgaacggca ttgccgatcg agagcttgtt gaccacgtcg
     5701 taaaaacgga ccttcatgcc gagagactcg gccatgacac cgagctgggt gccgatgttg
     5761 ccatagccga tgatgccgag ctgcttgccg cgaatctcgt aggcgttgct ggcgctcttc
     5821 agccagccgc cgcgatgggc cagtgcgttc ttttcgggaa tgccgcgcag cagcagaatc
     5881 gcctcggcca gcaccagctc cgccacgctg cgggtgttgg agaagggggc gttgaacacc
     5941 ggaatggcct gcaacagcgc ctggttcaga tcgacctggt tggtgccgat gcagaagcag
     6001 ccgatcgcca ccagccgctg cgccgcttcg agcacctcgg cgttgagccg ggtgcgtgag
     6061 cggatgccga tgaagtgcac gtcgcgaatc cgcgccttca gctcctcgcc ctcgagcgcg
     6121 gtcggcagtg cctcgatgtt gctgtagccg gcctggcgaa gggtctgcac ggccgagggg
     6181 tggatgccct cgagcagcag aatcttgatg cgatccttgt cgagcgagag tctggtcatg
     6241 gcaaggctcc tgataaagca acgggtgcct gtggctgcga ctgtgcagcc cacatggggg
     6301 gcgctatgct agcagcccgc cggaatcagg ccaaaccgcg ctcgcttgcg caccacgcgt
     6361 tccgcctcgg ctgcggcatc cgctaagatg gtcgcacctg gacggtcgag ggggtgacgc
     6421 agatgaacag cacgatcgtc gagcgcgcca cgcagctggg tctgctgctg aagcagcgcg
     6481 gtctgcgggt ggcgaccgcc gagtcctgca ccggtggtct ggtggccgcg gccatcaccg
     6541 ccgtggccgg cagctcggcc tggttcgagt atggctgggt ggtctactcc aacgaagcca
     6601 agcgcaccct gcttggcgtg gcggccgata cgctgcaacg gttcggtgcc gtcagccatg
     6661 aaaccgtgcg cgagctggcc cgcaatgcgc tgctgcgggc cgatgccgat ctggcggtgg
     6721 cggtcagtgg cattgccggg ccgggcggcg ccaccgcgca caagccggtc ggcacggtct
     6781 gcttcggctg ggccgggcgc gatggccggc tggagaccga gtcgctgcac ttcaccggtg
     6841 accgtgccgg ggtgcgcgag caggcggcgc tgcatgcgct ggccgggctg atggcgcggg
     6901 cctgacacac tttttcaatc aactgcaggc aagacggtgg agtgcgcact ccaccgcacc
     6961 atgaggaaag ccgatggatg caaacaagca gaaggcgctc gaagcggcgc tgggtcagat
     7021 cgagagacag ttcggcaagg gcagcatcat gcggctcggc gacaaggagc ggcagaacat
     7081 tccggcgatc tcgaccggtt cgctcgggct cgacatcgcg ctgggcatcg gtggcctgcc
     7141 gcgtggccgg gtggtggaga tctacggtcc ggaatcttcc ggcaagacca cgctggcgct
     7201 cagcaccatc gcccagtgcc agcgcggagg tggcgtggcc gccttcgtcg atgccgaaca
     7261 tgcgctcgac ccgctctatg ccgaaaagct cggcgtcaac ctcgacaacc tgctggtgtc
     7321 gcagcccgac accggcgagc aggcgctgga gatcgtcgac atgctggtgc gctccggcgc
     7381 ggtcgacctg gtggtgatcg actcggtggc ggcgctgacg cccaaggccg agatcgacgg
     7441 cgagatgggc gaccagcatg tcggcctgca ggcacggctg atgtcgcagg cgctgcgcaa
     7501 gatcaccggc aacatcaagc gttccaacac gctggtgatc ttcatcaacc agatccgcat
     7561 gaagatcggc gtgatgttcg gcagtccgga gaccaccacc ggcggcaatg ccctgaagtt
     7621 ctacgcctcg gtgcggctcg acatccgccg caccagctcg gtcaagtccg gcgacgaggt
     7681 ggtgggcagc gagacccggg tcaaggtggt gaagaacaag gtggcgccgc cgttccggca
     7741 ggccgagttc cagattctct acggcaccgg catctaccgc ctgggtgagc tggtcgacat
     7801 ggccgtgcag cagaatctga tcgaaaaatc gggcgcctgg tacagctgcc gtggcgagcg
     7861 catcggtcag ggcaaggcca acgccgccca gtatctgcac gagcatccgc aactggccaa
     7921 cgaactggag gcgcagattc gcagcctctg caccatcacc ccgaccgcca cggatgagtc
     7981 ggccgatgag ccgtgagtga ggcccacccc gctgcccacc gtgcctgtca gcgagctgcc
     8041 gccgtcattc agcctcgatg ccgaacgcgc ccggctggcg cactgccgcg ccagtgaccg
     8101 gatcaggctc gaggcactgc tcgggcgcat cgccagccgt ctggcggccg ccaagccggc
     8161 cgaccggctg ctgagcgagt tccggcagca gctcgacgcc tcgtcgagct gggtcgaggc
     8221 gcggctgcaa tcgataccca ccatcgacta tccgctcgaa ctgccggtgg tggccgcccg
     8281 cgaacggctg ctcgatgcca tcgagcggca tcaggtggtg gtgctggccg gcgagaccgg
     8341 ctcgggcaag accacccagt tgccgaagct ctgcctcgaa ctgggctgcg gcagccgtgg
     8401 gatgatcggc cacacccagc cgcggcggct ggcggcgcgg gcggtggcac aacggctggc
     8461 cgaggagctg cgggtgccgc tcgggcagca ggtgggcgcg caggtgcgct tcagcgacca
     8521 gagcggcccg cagacgctgg tcaaggtgat gaccgatggc atcctgctgg ccgagatcgg
     8581 ccatgatccg ctgctgaccg cctacgacac cctgatcatc gacgaggcgc atgagcgcag
     8641 cctcaacatc gacttcctgc tcggctatc
//
LOCUS       DAORCQ010000096         8600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5749_length_8600_cov_6.150497, whole genome shotgun sequence.
ACCESSION   DAORCQ010000096 DAORCQ010000000
VERSION     DAORCQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_8600_cov_6.150497"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /gene="narH"
                     /locus_tag="PKI23_09510"
     CDS             complement(<1..670)
                     /gene="narH"
                     /locus_tag="PKI23_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252563.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNN37213.1"
                     /translation="MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ESKPGIGYPRDWENQQRWNGGWRRKPNGRIEPRIGAKWRVLANIFANPDLPEIDDYYE
                     PFNFDYEHLQSARESQAFPTARPRSAITGERMEKIEWGPNWEEILGGEFAKRSADVNF
                     EGVQKEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCV"
     gene            complement(683..4423)
                     /locus_tag="PKI23_09515"
     CDS             complement(683..4423)
                     /locus_tag="PKI23_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HNN37214.1"
                     /translation="MSHFLDRLTFFRRTVGDFSAGHGIVTQEDRSWEEGYRKRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTREELPNHEPRGCARGASYSWY
                     LYSGARVKYPLIRGRLLRAWRTARATLPPVAAWAAIVEDPEQRRAYTAIRGHGGFVRA
                     GWDEITEIIAAANAYTVKRWGPDRVFGFSPIPAMSMISYAAGARYLQLLGGGCGSFYD
                     WYCDLPPASPQTWGEQTDVAESADWYNSGFLVLWGSNVPQTRTPDAHFYTEARYRGAK
                     SVVICPDYSEASKFADLWVAVKQGTDAALAMAFGHVILKEFHVDRQVPYFRDYLRKYS
                     DLPLLVRLVPQEGAYVAERLLRASDFDDGLGQGNNPEWKTVALDERSGEVVVPTGSIG
                     FRWGPDGRDDAGKWNLEEKDAAGRDTTLRLGLNGVHDAVAGVRFPYFANAASNGFAAT
                     DHPDVLVRNVPVKRLQLKEGEVLVASVYDLFLANYGVDQGFGGEHMPRDFDDPEPYSP
                     AWAEQITSVPREQILAVARGFADNAEKTNGRSMVIIGAAMNHWYHMDMNYRGVINMLV
                     MCGCVGQSGGGWSHYVGQEKLRPQSGWAPIAFALDWIRPPRQQNSTSFFYAHTDQWRY
                     ETVSTAEILSPTAPAGPWDASLIDFNARAERMGWLPSTPALKTNPLEVARAAAAAGVD
                     PVAYTVNELKSRRLELSCMDPDDPANWPRNLFIWRSNLLGSSGKGHEYFLKHLLGAPH
                     GVQGKDLGQEGRQKPREVAWHDEAPEGKLDLLVTLDFRMSTTAVYSDIVLPTATWYEK
                     NDLNTSDMHPFIHPLGAAVDPAWESKSDWEIFKAIAKAFSAVAPEVLGVEKDVVLSPI
                     QHDSAGELAQALDVKDWYLGECEPIPGKTMPQISVVERDYPNLYQRFTALGPLLDRLG
                     NAGKGLTWNTREDVERLRQINGAVHEAGATQGLARLERDIDVCEAILALAPETNGSVA
                     VKAWEALEQKTGRPHAHLALPKEDEKIRFHDLLAQPRKIISSPIWSGLESEKVCYTAG
                     YTNIHELIPFRTLSGRQQLYQDHLWMRAFGEALCLYKPPLDLKTTWVKGLKPNGEAEI
                     VLNFITPHQKWGIHSTYSDNLLMLTLNRGGPVVWLSETDAQKAGIRDNDWVEAFNANG
                     ALAARAVVSQRIREGTLFMYHAQEKIVNTPGSEITGLRGGIHNSCTRAVLKPTHMIGG
                     YAQQAYGFNYYGTVGSNRDEFVVVRKMAKVDWLEAPLDTRNP"
     gene            complement(4439..7171)
                     /locus_tag="PKI23_09520"
     CDS             complement(4439..7171)
                     /locus_tag="PKI23_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN37215.1"
                     /translation="MNSEAHPQSTRILSASTVAFTVCFAVWTIFSIIGIRIREELGLN
                     ETQFGLLVGMPILTGSLIRVALGVWSDLYGGRLVFTLTMLAAAVATWLLSQAETYPQI
                     LLAGLGVGIAGGSFAVGVAYVSRWYPQQKQGTALGIFGMGNIGAAVTKLLAPFALVAF
                     GWQSVAQIWAAGLAITAVLFWLTTQDDPVLVERRRAGIKAKSAWLELEPLKNVQVWRF
                     SLYYFFVFGAFVALALWLPRYLTQVYQLDIKSAGMIAALFSIPASFFRAYGGHLSDAY
                     GARRVLYWTFFVSIVATFLLSYPPTDYVVHGVKETFSFHLEMGLPSFVVIVFVLGFFM
                     ALGKAAVYKHIPVYYPEHVGAVGGLVGMIGGLGGFILPILFGALLDLTGLWTSCFMAL
                     FVLVLVAMVWMHVAILRMEREAAAPLLSTLPELPEMKAVHGARQVGALGPQRVLADWR
                     PEERTFWEQQGKSIARRNLWLSIPCLLLSFAVWMVWSVVVAKLPAIGFRYSTNELFWL
                     ASLPGLSGATLRIFYSFMVPIFGGRMWTTLTTWSLMIPAVGIGFAVQNPATPYWVFLL
                     LALLCGFGGGNFASSMSNISFFFPRAEKGNALALNAGLGNLGVSVVQFLVPLVITAGV
                     FGALGGDPQVATQGDKTVSLWLQNAGFIWVPFIAAAAIAAWFGMNDIASAKASFSEQS
                     VIFQRRHNWIMCWLYTGTFGSFIGYSAAFPLLTKTAFPAVNALQLAFLGPLVGALSRS
                     LTGWVADKYGGGRVTFWVFVAMMAGVGGVLYGLEMQSFPIFFASFLALFCATGIGNAS
                     TFQMIPAIMTKELQRLMPDVDAESRQRQVEKEAAAITGFTSAIAAFGAFFIPKSYGTS
                     IALTGGAQAALWCFLIFYVSCLVITWRVYTRKGGLLHDLERQRRAAPAQPAAAK"
     gene            7382..8095
                     /locus_tag="PKI23_09525"
     CDS             7382..8095
                     /locus_tag="PKI23_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNN37216.1"
                     /translation="MTVEEEIELQTVPPLCRLPQASRERLLASAIQHRVAAGTVLFEQ
                     GELPTFQIVVLAGSVQLFGRSTQGREVLVEVARAPDLIIPAAVVTGAPYLMQARVPEP
                     SRLLLIPAALFRDTLLQDPLLAHEVIDALARQFRRMVRQVKNLKLRSSVQRVGCYLLT
                     LSKRQGTPGQAVLPYEKNLIASELGITRESFSRALANLEQAGIRVNGQTIMIVDATRL
                     ATACQPDSLIDDIEGAVAV"
     gene            complement(8132..>8600)
                     /locus_tag="PKI23_09530"
     CDS             complement(8132..>8600)
                     /locus_tag="PKI23_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNN37217.1"
                     /translation="AERLNGLSAIEVREARHGDRVMPGRALIAPGGRHMTLKRSGAQY
                     HVEVIDGPPINRHKPSVDVLFRSVARAAGRNALGIIMTGMGDDGARGLKEMRDAGAMT
                     LGQDEASCVVYGMPKEAVRLGAVERSLSLTQMAGAIVEHGKQAATATAAHRAG"
BASE COUNT         1500 a         2817 c         2879 g         1404 t
ORIGIN      
        1 agacgcacat gcgccagccg cggcacttgt cctggtcgat gaggacgatg ccgtcttcct
       61 cgcgcttgta gatggagccc gagggacagc tcgcgacgca cgccgggttc aggcagtgct
      121 cgcacagccg cggcaggtac atcatgaagg tgttctcgaa ctggccgtag atctccttct
      181 gcaccccctc gaagttgaca tcggcgctgc gcttggcgaa ttcaccgccg agaatctcct
      241 cccagttcgg cccccattcg atcttctcca tgcgctcgcc ggtgatcgcc gagcgcgggc
      301 gcgccgtcgg aaaggcctgg gactcgcggg cgctctgcag gtgttcgtag tcgaagttga
      361 acggctcgta gtagtcgtcg atctcgggca ggtcgggatt ggcgaagatg ttcgccagca
      421 cccgccactt ggcgccgatg cgcggctcga tgcggccgtt cggcttgcgc cgccagccgc
      481 cgttccagcg ctgctggttc tcccagtcgc gggggtagcc gatgcccggc ttggattcga
      541 cgttgttgaa ccaggcgtac tcgacccctt cgcggctggt ccagacgttc ttgcaggtga
      601 ccgaacaggt gtggcagccg atgcatttgt cgaggttgag caccatgccg atctgtgcgc
      661 ggattttcat cgtgcaacct cctcaggggt tccgggtgtc gagcggcgct tcgagccagt
      721 cgactttcgc catcttgcgg accacgacaa actcgtcgcg gttggagccg accgtgccgt
      781 agtagttgaa gccataggcc tgctgggcat agccgccgat catgtgcgtc ggcttgagca
      841 ccgcgcgggt gcaggagttg tggatgccac cgcgcaggcc ggtgatctcc gagccggggg
      901 tgttcacgat cttctcctgc gcgtggtaca tgaacagtgt cccctcgcgg atgcgctgcg
      961 acaccaccgc gcgggcggcg agggcgccgt tggcattgaa ggcctcgacc cagtcgttgt
     1021 cgcggatgcc ggccttctgc gcgtcggttt ccgagagcca gaccaccggc ccgccgcggt
     1081 tcagtgtcag catcagcagg ttgtcggagt aggtggagtg gatgccccac ttctggtgcg
     1141 gggtgatgaa gttgagcacg atctcggcct cgccgttcgg cttgaggccc ttcacccagg
     1201 tggtcttcag gtcgagcggc ggcttgtaga gacagagcgc ctcgccgaag gcgcgcatcc
     1261 acaggtggtc ctgatagagc tgctgccggc cgctgagggt gcggaatgga atcagctcgt
     1321 ggatgttggt gtagccggcg gtgtagcaga ccttctcgct ttcgaggccg gaccagatcg
     1381 gcgaggagat gatcttgcgc ggctgggcca gcaggtcgtg gaagcggatc ttctcatcct
     1441 ccttcggcag cgccagatgg gcatgcgggc ggccggtctt ctgctccagc gcctcccacg
     1501 ccttcaccgc aacgctgccg ttggtctccg gcgccagcgc cagaatcgcc tcgcagacat
     1561 cgatgtcgcg ctcgagccgc gccaaacctt gggtcgcgcc tgcctcatgc accgcgccgt
     1621 tgatctgccg cagccgctcg acatcctcac gggtgttcca ggtgagcccc ttgccggcat
     1681 tgcccagccg gtcgagcagc ggccccagcg cagtgaaacg ctggtagagg ttggggtagt
     1741 cgcgctcgac cacgctgatc tgcggcatgg tcttgccggg aatcggctcg cactcgccca
     1801 gataccagtc cttcacgtcg agcgcctgcg ccagctcgcc ggcgctgtcg tgctggatcg
     1861 ggctcagcac cacatccttc tcgaccccca gcacctcggg cgcgacggcc gagaatgcct
     1921 tggcgatcgc cttgaagatc tcccagtccg atttgctctc ccaggccggg tccaccgccg
     1981 caccgagcgg gtggatgaag gggtgcatgt cggaggtgtt gaggtcgttc ttctcatacc
     2041 aggtcgcggt cggcagcacg atgtcggagt agaccgcggt ggtcgacatg cggaaatcga
     2101 gcgtcaccag caggtcgagc ttgccctcgg gggcctcgtc gtgccaggcg acctcgcgcg
     2161 gcttctgccg cccctcctgc cccagatcct tgccctgcac cccgtgggga gcgcccagca
     2221 ggtgcttgag gaagtactcg tgccccttgc cggaggagcc aagcagattg gagcgccaga
     2281 tgaacaggtt gcgcggccag ttggccggat cgtcggggtc catgcaggag agttcgagcc
     2341 ggcgcgactt gagctcgttg acggtgtagg ccaccgggtc gaccccggcc gcggcagccg
     2401 cccgcgccac ctccagcgga ttggtcttga gcgccggcgt cgagggcagc cagcccatcc
     2461 gttcggcgcg ggcgttgaag tcgatcagcg atgcatccca aggccccgcc ggcgccgtcg
     2521 gcgacaggat ctcggcggtg ctgacggtct cgtagcgcca ctggtcggtg tgggcataga
     2581 agaacgaggt cgagttctgc tggcgcggtg ggcgaatcca gtcgagggcg aaggcgatcg
     2641 gcgcccagcc ggactgcgga cgcagcttct cctggccgac gtagtggctc cagccaccgc
     2701 cggactggcc cacgcagccg cacatcacca gcatgttgat gacgccacgg tagttcatgt
     2761 ccatgtggta ccagtggttc atcgccgcgc cgatgatgac catcgaccgg ccattggtct
     2821 tctcggcgtt gtcggcaaag ccgcgcgcca cggccagaat ctgctcgcgc ggcacgctgg
     2881 tgatctgctc ggcccaggcc ggcgagtagg gttctggatc gtcgaaatcg cgcggcatgt
     2941 gctcgccgcc aaagccctga tcgacgccgt agttggccag aaacaggtcg tagaccgagg
     3001 ccaccagcac ctcgccctcc ttcagctgca gccgcttgac cggcacgttg cgcaccagca
     3061 catcggggtg gtcggtcgcg gcgaagccgt tgctggccgc gttggcgaaa tagggaaagc
     3121 gcacccccgc caccgcgtca tgcaccccgt tgaggccgag gcgcagcgtg gtgtcgcggc
     3181 cggcggcatc tttttcttcg aggttccatt tgccggcgtc gtcgcgcccg tccgggcccc
     3241 agcgaaagcc gatcgagccg gtgggcacca ccacctcgcc gctgcgctca tccagcgcca
     3301 ccgtcttcca ttcggggttg ttgccctgcc ccagcccatc gtcgaagtcg gaggcgcgca
     3361 gcaggcgctc ggcgacatac gccccctcct gcggcaccag ccgcaccagc agcggcaggt
     3421 cggagtattt gcgcaggtag tcgcggaagt agggcacctg ccggtcgacg tggaactcct
     3481 tcaggatcac atggccgaac gccatcgcca gcgcggcgtc ggtgccctgc ttcacggcga
     3541 cccacaggtc ggcgaatttc gacgcctcgg agtagtcggg gcagatgacc acgctcttgg
     3601 caccccggta gcgcgcctcg gtgtagaagt gggcatcggg ggtgcgggtc tggggcacgt
     3661 tggagcccca cagcaccagg aagccggagt tgtaccagtc cgcgctctcg gcgacgtcgg
     3721 tctgctcgcc ccaggtctgc ggcgaggccg gcggcaggtc gcaataccag tcgtagaacg
     3781 agccgcagcc gccgcccagc agttgcaggt agcgcgcccc ggcggcgtag gagatcatcg
     3841 acatcgccgg aatcggcgag aagccgaaca cccggtcggg cccccagcgc ttgacggtgt
     3901 aggcgttggc ggcggcgatg atctcggtga tctcgtccca gccggcgcgc acgaagccgc
     3961 cgtggccgcg gatcgcggtg taggcgcgcc gctgctcggg atcctcgacg atggcggccc
     4021 aggccgcgac cggcggcagc gtggcccgcg cggtgcgcca tgcgcgcagc agccggccgc
     4081 ggatcagcgg gtacttcacc cgggcgccgg agtagagata ccagctgtag ctggcgccac
     4141 gcgcgcagcc gcgcggctcg tggttgggca gctcctcacg ggtgcgtggg tagtcggtct
     4201 gctgcgtctc ccaggtgacg atgccgccct tgacatagat tttccacgag caggagccgg
     4261 tgcagttcac gccatgggtg gagcgcacga tcttgtcgtg ctgccagcgc ttgcggtagc
     4321 cctcctccca gctgcgatcc tcctgggtca cgatgccgtg gcccgcggaa aaatcaccga
     4381 cggttctgcg aaagaaggtg agccgatcga gaaagtgcga catggtgagg gtccctggtt
     4441 acttggccgc agccggttga gcaggtgcag cgcgccgctg ccgttcgagg tcgtgcagca
     4501 ggccgccctt gcgggtgtag acccgccagg tgatgaccag acagctgacg tagaagatca
     4561 ggaagcacca gagcgccgcc tgtgcgccac cggtcagcgc gatcgaggtg ccatagctct
     4621 tcgggatgaa gaaggcaccg aaggcggcga tcgccgaggt gaagccggtg atggccgccg
     4681 cctccttctc gacctgccgc tggcggctct cggcgtcgac gtcgggcatc agccgctgca
     4741 gctccttggt catgatcgcc gggatcatct ggaaggtgga ggcattgccg atgccggtgg
     4801 cgcagaacag cgccaggaag ctggcaaaga agatcgggaa gctctgcatc tccagcccgt
     4861 agagcacacc gccgacgccg gccatcatcg ccacgaagac ccagaaggtg acgcgcccgc
     4921 caccgtattt gtccgccacc cagccggtca gtgagcgcga cagcgcgccg accagcggac
     4981 cgagaaaggc cagttgcagg gcgttgaccg ccgggaatgc ggtcttggtc agcagcggga
     5041 aggccgccga gtaaccgatg aaggagccga aggtgccggt gtaaagccag cacatgatcc
     5101 agttgtgtcg ccgctggaag atgaccgact gctcggagaa cgacgccttg gccgaggcga
     5161 tgtcgttcat gccgaaccag gcggcgatcg ccgcggcggc gatgaagggc acccagatga
     5221 agccggcatt ctgcagccac aggctcactg tcttgtcacc ctgggtcgcg acctgcggat
     5281 caccccccag cgcgccgaac acaccggcgg tgatcaccag cggcaccagg aactgcacca
     5341 cgctgacccc gaggttgccg agcccggcgt tgagtgccag cgcattgccc ttctccgccc
     5401 gcgggaagaa gaacgagatg ttcgacatcg aggaggcgaa gttgccgccg ccaaagccgc
     5461 acaacagcgc cagcagcaga aagacccagt agggggtggc cggattctgc acggcaaagc
     5521 cgatgccgac ggccgggatc atcagcgacc aggtggtcag cgtcgtccac atccgtccgc
     5581 cgaagatcgg caccatgaag gagtagaaga tgcgcagcgt cgcgcccgac agtcccggca
     5641 gcgaagcgag ccagaacagc tcattggtgc tgtagcgaaa gccgatggcc ggcagcttgg
     5701 cgaccaccac cgaccagacc atccacaccg cgaacgacag cagcagacag gggatcgaca
     5761 gccagaggtt gcggcgggcg atcgatttgc cctgctgctc ccagaaggtg cgctcctcgg
     5821 gccgccagtc ggccagcacc cgctgtggcc ccagcgcacc cacctgtctg gcaccatgca
     5881 cagccttcat ctccggcagc tccggcagcg tgctgaggag gggggccgcc gcctcgcgct
     5941 ccatgcgcag gatcgccaca tgcatccaga ccatcgcgac cagcaccagc acgaacagcg
     6001 ccatgaagca gctggtccac agcccggtca ggtcgagcag ggcgccgaac aggatcggca
     6061 gaatgaagcc gcccaggccg ccgatcatgc cgaccagacc acccaccgcg ccgacatgct
     6121 ccgggtagta gaccgggatg tgcttgtaga ccgccgcctt gcccaaggcc atgaagaaac
     6181 cgagcacgaa gacgatgacg acgaaactgg gcagccccat ttcgaggtgg aacgagaagg
     6241 tctccttcac cccgtgcacc acgtagtcgg tcggtggata ggagagcaga aaggtggcga
     6301 cgatggagac gaagaaggtc cagtagagca cccgccgcgc gccgtaggca tcggagaggt
     6361 ggccaccata ggcgcggaag aagctggccg gaatcgagaa cagcgccgcg atcatcccgg
     6421 cactcttgat gtcgagctgg tagacctggg tcaggtagcg cggcagccag agcgccaggg
     6481 cgacaaaggc gccgaagaca aagaagtagt agagcgagaa gcgccacacc tgcacattct
     6541 tcagcggctc cagctcgagc caggcgcttt tggccttgat gccggcacgg cgacgctcga
     6601 ccagcaccgg gtcatcctgg gtcgtcagcc agaacagcac ggcggtgatg gccaggcccg
     6661 ccgcccagat ctgcgcgacg ctctgccagc cgaacgccac cagcgcgaac ggggcgagca
     6721 gcttggtgac ggcggcgccg atgttgccca tgccgaagat gccgagcgcc gtgccctgct
     6781 tttgttgcgg ataccagcgc gagacatagg caacgccgac cgcgaaggag ccgccggcaa
     6841 tgccgacccc cagccccgcc agcaggatct gcggataggt ctcggcctgc gacagcagcc
     6901 aggtggccac ggcggcggcc agcatcgtca gcgtgaagac cagccggccg ccgtagaggt
     6961 cgctccagac ccccagcgcc acccggatca gcgagccggt cagaatcggc atgccgacca
     7021 gcaggccgaa ctgggtctcg ttcagaccca actcttcgcg aatgcggatg ccgatgatcg
     7081 aaaagatggt ccatacggcg aagcagacgg taaaggccac cgtgctcgcg ctcaggatgc
     7141 gggtcgattg gggatgggcc tcgctgttca tcgctcgatt tccttctgtc gatggggggt
     7201 cggaaaacgg tgagcctgca tcaggctcgc gactacggtg acaggacctc gcagctcttt
     7261 cattgatcca aatcaaatgt tgccgtgaca tttgtcacaa tacttgtcgc caagcaaaat
     7321 cggccgatgc agcggatcaa gccctgaaaa catttcgcag cccagcgagg agaacccaac
     7381 agtgaccgtg gaggaggaga tcgaactgca gacggtaccg ccgctgtgcc ggttgccaca
     7441 ggccagccgc gagcggctgc tggccagcgc gattcagcac cgggtggcgg cgggaacggt
     7501 gctgttcgag cagggcgaac tgcccacctt ccagatcgtg gtgctggccg gttcggtgca
     7561 gctgttcggc cgttcgaccc agggtcgtga ggtgctggtg gaggtggcgc gcgcgccgga
     7621 cctcatcatt ccggccgcag tggtcacggg tgcgccctat ctgatgcagg cgcgggtgcc
     7681 ggagccttcg cgcctgctgc tgattccagc ggcgctgttt cgcgacaccc tgctgcagga
     7741 tccgctgctg gcccacgagg tgatcgatgc gctggcacgg cagttccggc gcatggtgcg
     7801 gcaggtgaag aacctgaagc tcaggtcgtc ggtgcagcgg gtgggctgct atctgctgac
     7861 gctgtcgaag cgccagggca cgccggggca agcggtgctg ccctatgaaa aaaatctgat
     7921 cgcctctgag ctcggcatca cccgcgaatc gttttcgcgt gcgctcgcca acttggagca
     7981 ggccggcatc cgggtgaatg gacagaccat catgatcgtc gacgcgacac ggctggcaac
     8041 ggcctgtcag cccgattcgc tgatcgatga catcgagggg gccgtggcag tgtgagcctg
     8101 ccgcgctgtc ggggcaaaaa tcgaaccgcg ctcaccccgc ccggtgagca gcagtggccg
     8161 tcgcggcctg cttgccatgc tcgacgatgg cgccggccat ctgcgtcagc gacagcgagc
     8221 gttcgaccgc gcccagcctg accgcctcct tgggcatgcc atagaccaca caactggctt
     8281 cgtcctgtcc caacgtcatg gcaccggcat cgcgcatctc cttcaggcca cgcgcaccat
     8341 catcgcccat gccggtcatg atgatgccga gcgcattgcg tccggcggca cgggccactg
     8401 aacggaacag cacgtcgacc gagggtttgt ggcggttgat cggtggaccg tcgatcacct
     8461 cgacatggta ctgggccccg ctgcgcttga gtgtcatgtg ccgtccaccc ggcgcgatca
     8521 gcgcgcggcc cggcatcacc cggtcaccat gccgcgcctc acgcacctcg atggccgaca
     8581 gcccgttcag ccgctcggca
//
LOCUS       DAORCQ010000097         8395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_5955_length_8395_cov_5.550959, whole genome shotgun sequence.
ACCESSION   DAORCQ010000097 DAORCQ010000000
VERSION     DAORCQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5955_length_8395_cov_5.550959"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..946)
                     /locus_tag="PKI23_09535"
     CDS             complement(206..946)
                     /locus_tag="PKI23_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNN37218.1"
                     /translation="MIQSHRSPLAVASLAASLLLGSSAAMAATSFTYDFESGTVDAEW
                     SMISPGSLAVDTTPSGRKFLGLNDSQNLGFSNDAVKLTLTGLPSYSGFTLSFDLYAIQ
                     SLDGNQFGYGPDLIGATATSGSLSLPLLHTTFSNIPSYNQSYPGNFFSSNPAQSGSAE
                     QNSLGYSYFGDSVYQLSFGWGFGQTNAPLEVVFFASGLQGIGDESWGIDNVRIELSGT
                     PAPVPLPPAVWLLGTGLISLAGIARRNT"
     gene            1185..1886
                     /locus_tag="PKI23_09540"
     CDS             1185..1886
                     /locus_tag="PKI23_09540"
                     /inference="COORDINATES: protein motif:HMM:NF019378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HNN37219.1"
                     /translation="MSRNGLFLGPPGCLALLLLFGLLVLVPIFLANAAVTALAKLGLS
                     PGVSLLALIGIVVGGLINIPLYRLPTYLVVDAPAPQLFGLDRLRPQRTTFEEGFMVLA
                     VNVGGCLVPLAIALYELVRLAGAGGGMLLWTVLAVVINVLLCQRLAKLLPGRGVALPP
                     LLPALTAVLCAWILGGEMAAPVAFVAGVLGPLIGADLLNLPRVRQLGAPIASIGGAGT
                     FDGIVLSGLLATLLA"
     gene            complement(1893..2411)
                     /locus_tag="PKI23_09545"
     CDS             complement(1893..2411)
                     /locus_tag="PKI23_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNN37220.1"
                     /translation="MTRELLLMRHAKSDWPPDVADFNRPLTKRGRADSQKMADWLQAR
                     QLLPELVIASTAARAAETCQLLCARLELPPTRLRWEPRIYEATPTTLLTRLHTTPSSC
                     RRLLLIGHNPGLEQLLLTLAQDQIAVRADGKLMTTAAIARIQLDCDWSEVRAKCGRLI
                     SLIRPKEIEQHS"
     gene            complement(2514..3479)
                     /locus_tag="PKI23_09550"
     CDS             complement(2514..3479)
                     /locus_tag="PKI23_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN37221.1"
                     /translation="MSDPLALLSLTAIEQRFTSGSGLQRRTIRAVDGVSLQIRPGEVL
                     GLVGESGCGKSTLGRIAVGLTAPASGEVRYRGQPIRQLHGDDWQQFRRRVQMVFQNPH
                     ASLSPRLRIEAQLLEPIRWHHPTASAAEHRQQLESLMAAVGIDTAWRQRYPHQLSGGQ
                     KQRIAIARALTTTPELIVADEPVSALDLSVQAQVLNLLLELRASRGLTYLFISHDLAV
                     VRHIADRVAVMYLGTLCELAPAATLFSAPRHPYTQALLQAIPRLDRDRPNPPRLAAEL
                     PNPGTTLIGCPFHSRCPQAQALCRQQRPALRQIAAEHQVACHLLE"
     gene            complement(3476..4450)
                     /locus_tag="PKI23_09555"
     CDS             complement(3476..4450)
                     /locus_tag="PKI23_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN37222.1"
                     /translation="MTPLLEVDRLEVSLGRTPLLRQLSFTLQAGERLGIVGESGAGKS
                     MLAHAVMNLLPPPAHISSGALRFMQQDLRTLDPAALHRLRGRQMAMIFQDPMMTLNPL
                     LTIGTQLTECLTHPPAGVERISARAARVRAIEALQEVRIPAAASQLQAYPHQLSGGMR
                     QRVAIAIALLHRPALLIADEPTTALDVTIQAEILALLTELCTAQRMALMLITHDLAVL
                     AETTDHTLVLYAGEAVETAPTRALIERPRHPYSRALLDALPQRATPGQPLRALGGSMP
                     TASALPSGCAFHPRCPQRMSVCPQQPPQWQGTAPDRQRRCHLPIEVDG"
     gene            complement(4447..5358)
                     /locus_tag="PKI23_09560"
     CDS             complement(4447..5358)
                     /locus_tag="PKI23_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN37223.1"
                     /translation="MNDRSSSLGRQLLRAPVALAALLLLLALVTASLAAQWLAPQNPY
                     DPASYDIMDAQIPPLWLAGGDPRFLLGSDGQGRDILSIILHGSRISLLIGLGAVAVQA
                     LLGIVIGLCAGYGSRWLDDLLMRLADVQMSISTLMIALVALALAQSLLPAEHYQHHVP
                     LLLILVIGLAEWPQYARTLRAAVLAEKQLAYVEAARLLGFSQRRILLRHLLPNTLSPL
                     LVISTVQVASAIMSEAALSFLGLGMPITRPSLGALINSGFELLLSGAWWISIIPGLTL
                     VLLILCINLLGDWLRDALDPRHGGHAP"
     gene            complement(5355..6332)
                     /locus_tag="PKI23_09565"
     CDS             complement(5355..6332)
                     /locus_tag="PKI23_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN37224.1"
                     /translation="MLELLLRRLIQTLLVLGVISLIAFVIQEQLGDPLRELSGQSVST
                     EERTRLRHELGLDRPFALRYGQFVGRALRGDLGHSYFFKEPALPLILARLPATLELVL
                     GATLIVLLISVPVGLFAAIRPRHPLSQLAMGLTLFGISVPIFITAILGIMLFSVKLGW
                     LPAQGRGELSAPFGWNSGLFTLDGVQHLLLPACTLASLLLPLFVRLIRAEMTAVLNQE
                     YIKFAWAKGLAPGRIWWLHAFKNTLLPLITVGGMQIGGLVAYAILTETIFQWPGMGAL
                     FLQSVNRADGPVVTAYLLVVGVIFVITSTLVDLLYGLVDPRIRLSGTAP"
     gene            complement(6367..7923)
                     /locus_tag="PKI23_09570"
     CDS             complement(6367..7923)
                     /locus_tag="PKI23_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNN37225.1"
                     /translation="MRTRWSRLLLMGLLVALCTTAAAQHTLRVAYDADPDSLDPHEQL
                     SGATLQLSHLLFDPLLRWKQDRSFEPRVAERWERIDPLTTRFHLRPGVKFHSGRPLTS
                     QDVAWTFARLQRSPDFKGLFAPFKPVRVIDDHTFELITHQPYPLVLNLATYIFVLDRQ
                     FYQGQDEQGRNKEALVKSGDSFAARNVSGSGPFRLVSRQQGVRLELQRFADHWDRQSP
                     GNVERIVLTPIKEDATRIAALLAGDVDFIAPVPPADLPRLQRQPAIEVVTMTGTRIIT
                     LQLNQQRVPAFADPRVRRAVRLAINNVGISEKIMRGFATPAAQLSPTGYPGHDPALVP
                     RFDLAEARQLLQQAGHGQGLKVTMLAPNNRYVNDEQIAQAVVGMLAKIGIGVELKTVP
                     KAQYWPLFDQSAADMLMLGWQSDTEDSGNFFEFMLMTHDSQNGYGQYNSGHYRNPALD
                     ALLLQAQTELDPARRAQLLQQAERLVHDDAAIIPLHWQNLAWAARRGVRIDRIVNVMD
                     FPYLGDLVIE"
     gene            complement(8138..>8395)
                     /locus_tag="PKI23_09575"
     CDS             complement(8138..>8395)
                     /locus_tag="PKI23_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNN37226.1"
                     /translation="GNRDEEVIDRPYEFIIDRENPRHHLSFGFGIHRCMGNRLAEMQL
                     RVTWEEIMKRFKTIEVVGEPVRNYSPFIKGYSELPVQLRPL"
BASE COUNT         1507 a         2478 c         2933 g         1477 t
ORIGIN      
        1 cacagcgcgc gggcgccggc catgttgcgg ccatgggtgg aggtcttgga gcggtaactg
       61 ggcatggtgt cggttccatg ggacaaggag gagggcggca ttgtagcaaa ggcatcgttc
      121 agcacggccg gtaatgcagt tgcttcagac agcaaaaggc ccgccggcaa gatgcgcggc
      181 gggccttgaa gcagccagag gaagatcagg tgttgcgacg ggcgatgcct gccagcgaaa
      241 tcaggccagt gccgagcagc cagaccgcag gtggcagcgg tacgggtgcc ggggttccgc
      301 tcagctcgat cctgacattg tcgatgcccc agctttcgtc gccgatgccc tgcaggcccg
      361 aggcgaagaa gacgacctca agcggtgcat tggtctggcc gaatccccag ccaaagctga
      421 gttggtagac cgaatcgccg aagtaggaat agccgaggct gttctgttca gccgaaccgc
      481 tctgagcggg gttgctggag aaaaaattgc ccgggtagga ctggttgtag cttggtatgt
      541 tggaaaaagt ggtgtgcagc agcggcaagg agagcgagcc actggtggcg gtggcgccga
      601 tcaggtccgg accatagccg aactgattgc catccagact ctggatcgca tagagatcga
      661 agctgagggt gaagccgctg taactgggca gcccggtgag cgtcaacttg acggcatcat
      721 tgctgaagcc gaggttctga ctgtcattga gtcccagaaa tttgcggccg ctcggggtgg
      781 tgtcgacggc cagcgagccc ggtgaaatca tcgaccattc ggcatcgacg gtgccgcttt
      841 cgaagtcata ggtgaaactg gtggcggcca tggcggcaga gctgcccagc agcagcgaag
      901 cagccaagga ggcgacggcc agaggtgagc gatgagactg aatcatgaca ttgtcctgct
      961 gagtggttag tgccaatgtt aaagcactat agatgccatt aaatttatat tattaaaaat
     1021 catatagtta ataaaaaaaa taatgatttt ttaatcgttt tttgatcgaa agtgtaaaaa
     1081 aaattgacac tcgccaggcg tgcggtcatt ccgctcggca ggtttgccgc aggccggtta
     1141 cactgcatcc cgttgtggaa aatgagtctg atcggaggag ttggatgtca agaaatggtc
     1201 tgttcctcgg cccccccggc tgcctggcac tgctgttgct gttcggtctg ctggtgctgg
     1261 tgccgatctt tctggccaat gcggcggtga ccgcgctggc gaagctgggg ctgagtcccg
     1321 gcgtctcgct gctggcgctg atcggcatcg tggtcggtgg gttgatcaac attccgctgt
     1381 accgcctgcc gacctatctg gtggtggatg cgccggcacc gcaactgttt ggtctcgatc
     1441 ggttgcgtcc gcaacgcacc acctttgaag agggcttcat ggtgctggcg gtcaatgtcg
     1501 gcggctgtct ggtgccgctg gccatcgcgc tctatgagct ggtgcgactg gccggtgccg
     1561 gcggcgggat gctgctgtgg acagtgctgg cagttgtcat caatgtgctg ctctgccagc
     1621 ggttggcgaa actgctaccg ggccgtggcg tggccttgcc gccgttgctt ccggcgctga
     1681 cggcggtgct ctgcgcctgg atactgggtg gcgagatggc cgcgccggtc gccttcgtgg
     1741 ccggggtgct ggggccgctg atcggggctg atctgctcaa tctgcccagg gtgcgtcagt
     1801 tgggggcgcc gattgccagc atcggcggcg cgggcacctt cgatggcatc gtgctctcgg
     1861 ggctgctggc cacgctgctg gcgtagccga catcaggagt gctgctcgat ctccttgggc
     1921 cggatcaggc tgatcagccg gccgcacttc gcccgcactt cactccagtc gcagtcgagc
     1981 tgaatccggg cgatggcggc cgtggtcatc agcttgccat cggcgcgcac ggcgatctga
     2041 tcctgcgcca gcgtcagcag cagttgctca agccctggat tgtgtccgat cagcagcagt
     2101 cgccggcagc tggaaggggt ggtgtgcagc cgtgtcagca gggtggtcgg ggtggcttca
     2161 tagatgcgtg gctcccagcg caaccgggtc ggtggcagtt caagccgggc gcagagcagt
     2221 tggcaagtct cggctgcgcg tgccgccgtg gaggcgatga ccagttccgg cagcagttgc
     2281 cgtgcctgca accagtcggc catcttctgg ctgtcggcac ggccgcgctt ggtcagcgga
     2341 cggttgaagt cggcgacatc gggcggccag tccgatttgg catggcgcat cagcagcagt
     2401 tcgcgggtca tgatcgaagg tttctgcgag gtgattgaac gtctggatgg atgatagagg
     2461 attgaacacc aaggtgtcag ggcatgttgc tctggcatct gaacgagacg gcttcactcc
     2521 agcagatggc aggcgacctg atgctctgcc gcgatctggc gcagggctgg acgctgctgc
     2581 cggcagagcg cctgcgcctg cggacagcgg ctgtgaaatg gacagccgat cagggtcgtg
     2641 ccggggttgg gcagttcggc agcgaggcgc ggtgggttgg ggcggtcgcg gtcgagccgt
     2701 ggaatcgcct gcagcagtgc ctgggtatag ggatggcgtg gggcgctgaa cagcgtcgcg
     2761 gccggggcca gttcgcacag ggtgccgaga tacatcaccg ccacccggtc ggcgatgtgg
     2821 cgcaccactg caaggtcgtg gctgatgaag aggtaggtca gtccgcgtga agcccgcagc
     2881 tccagcagca ggttgagcac ctgggcctgc accgacagat cgagtgccga gaccggctca
     2941 tcggcgacga tcagttcggg cgtggtggtg agggcgcggg cgatggcgat gcgctgtttc
     3001 tgaccgccgg agagctggtg tggatagcgc tggcgccagg cggtgtcgat gccgaccgcg
     3061 gccatcaggc tttcgagctg ctgccgatgt tccgccgcag aggcagtggg gtggtgccag
     3121 cggatcggct ccagcagttg ggcttcgatc cgcagccgtg gcgagagcga ggcgtgcgga
     3181 ttctggaaca ccatctgcac ccgccggcgg aactgctgcc agtcatcgcc atgcagttgc
     3241 cggatcggct ggccacggta gcggacttcg ccggacgctg gcgcggtcag gccgacggcg
     3301 atgcgcccca gcgtcgattt gccgcagccg ctctcgccga ccagtcccag cacctcgccg
     3361 gggcggatct gcagcgagac gccatcgacc gcccggatgg tgcgacgctg caggccgctg
     3421 ccgctggtaa agcgctgttc gatggcggtc aggctgagca gcgcaagggg gtcgctcatc
     3481 catcgacctc gatcggcagg tgacagcggc gctgtcgatc aggcgcagtg ccttgccact
     3541 gcggtggctg ttgcgggcag accgacatgc gctgcgggca acgtggatga aaggcacaac
     3601 cagagggcag ggcgctggcg gtcggcatgc tgccacccag ggcgcgcagc ggttggccgg
     3661 gcgtggcccg ctgcggcagc gcatcgagca gggcgcggct gtaggggtgg cgtggccgtt
     3721 cgatcagggc gcgggtcggc gcggtttcga ccgcctctcc ggcatagagc accagcgtgt
     3781 ggtcggtggt ctcggccagc acggcgaggt cgtgggtgat cagcatcagt gccatccgct
     3841 gtgcggtgca gagttcggtc aacagcgcca gaatttcggc ctggatggtc acatcgagcg
     3901 cagtggtcgg ttcgtcggcg atcagcaggg ccggccgatg cagcagcgcg atggcgatcg
     3961 ccacccgttg gcgcatgccg ccggagagct gatgcggata ggcttgcaac tggctggcgg
     4021 ccgcaggaat ccgcacttcc tgcagcgcct cgatggcgcg gacgcgggcc gcccgggcgc
     4081 tgatgcgctc gacccccgct ggcggatggg tcaggcattc ggtcaactgg gtgccgatgg
     4141 tcagcagcgg attcagcgtc atcatcggat cctgaaagat catcgccatc tgcctgccgc
     4201 gcaggcgatg cagcgccgct gggtcgagtg tccgcagatc ctgctgcatg aagcgcagcg
     4261 cgccgctgct gatatgcgcc ggcggcggca gcaggttcat cactgcatgg gccagcatcg
     4321 acttgccggc gccactctcg ccgacgatgc cgaggcgctc gccggcttgc aaagtgaagc
     4381 tgagctgtcg cagcagcggc gtgcgaccca ggctcacttc caatcggtcg acctcgagca
     4441 gtggcgtcat ggcgcgtgac cgccatgacg tggatcgagc gcatcacgca gccagtcacc
     4501 cagcaggttg atgcagagaa tcagcagtac cagcgtgagg ccagggatga tcgagatcca
     4561 ccaggcgccg ctcagcagca gctcgaaacc gctgttgatc agggcgccga gcgaagggcg
     4621 ggtgatcggc atgccgaggc cgagaaagga gagggcggct tcgctcatga tcgcgctggc
     4681 cacctgcacc gtggagatca ccagcagcgg cgacagggtg ttgggcagca ggtggcgcag
     4741 caggatccgg cgctggctga agccgagcag ccgtgccgcc tcgacgtagg cgagctgctt
     4801 ctcggccagt accgccgcgc gcagcgtgcg ggcgtactgc ggccactcgg cgagcccgat
     4861 caccagaatc agcagcagtg ggacatggtg ttgatagtgc tcggccggca gcagcgactg
     4921 tgccagcgcc agcgcgacca gcgcgatcat cagggtcgag atcgacatct gcacgtcggc
     4981 cagccgcatc agcaggtcgt cgagccagcg gctgccatag ccggcgcaga gaccgatcac
     5041 aatgccgagc agcgcctgca ccgccacggc acccaggccg atcagcaacg agatgcggct
     5101 gccgtgcaga atgatgctga gaatgtcgcg tccctgccca tcgctgccga gcagaaagcg
     5161 cgggtcgccg ccggccagcc acagtggcgg gatctgcgca tccatgatgt cgtaactggc
     5221 cggatcatag ggattctgcg gcgccagcca ctgcgccgcc aggctggccg tcaccaatgc
     5281 cagcaacagc agcaacgcgg cgagcgccac cggcgcgcgc agcagctgtc ggcccagact
     5341 ggatgaccgg tcgttcatgg cgcggttccg ctcagtctga tgcgtgggtc gaccaagcca
     5401 tagagcagat cgaccagggt actggtgatg acgaagatca caccgaccac cagcaggtag
     5461 gcggtcacca ccgggccatc ggcgcggttg accgactgca ggaagagcgc gcccatgccc
     5521 ggccactgaa aaatggtttc ggtcaggatg gcgtaggcca ccaggccacc gatctgcatg
     5581 ccgccgaccg tgatcagcgg cagcagcgtg ttcttgaatg catgcagcca ccagatgcgc
     5641 ccaggcgcga gccccttggc ccaggcgaac ttgatgtact cctgattgag cactgcggtc
     5701 atctcggcgc ggatcagccg cacgaacagc ggcaacagca gagaggccag cgtgcaggcc
     5761 ggcagcagca gatgctgcac gccgtcgagg gtaaagaggc cggagttcca gccgaagggt
     5821 gcgctcagtt cgccgcgtcc ctgcgccggc agccagccca gtttgaccga gaacagcatg
     5881 atgccgagga tcgcggtgat gaaaatcgga acagagatgc cgaacagggt cagccccatg
     5941 gcgagctgac tgagtggatg acgcggccgg atggcggcga acagcccgac cggaacgctg
     6001 atcaggagca cgatcagggt tgcgcccagc accagctcca gcgtggccgg tagccgcgcc
     6061 agaatcagcg gcaacgccgg ctccttgaag aagtaggagt ggccgaggtc accgcgcagg
     6121 gcgcggccga cgaactggcc atagcgcagt gcaaagggtc gatcgaggcc cagttcgtga
     6181 cgcagccggg tccgctcttc ggtcgacacc gactgcccgc tcagctcccg cagcgggtca
     6241 ccgagttgct cctggatcac gaaggcgatc aggctgatca ctcccagcac cagcagggtc
     6301 tggatcaggc ggcgcagcag cagttcaagc atcggtgagc tggatccact tccgttcggc
     6361 acaggactac tcgatcacca gatcgcccag gtagggaaaa tccatcacat tgacgatccg
     6421 gtcgatcctg accccacggc gggcggccca ggcgaggttc tgccaatgca gcggaatgat
     6481 cgccgcatcg tcgtgcacca gccgttcggc ctgctgcaac aactgtgcgc gccgtgcggg
     6541 gtcgagttcg gtctgcgcct gcaacagcag cgcatcgagg gccggattgc ggtagtggcc
     6601 actgttgtac tgcccgtagc cgttctggct gtcgtgggtc atcagcatga attcgaagaa
     6661 gttgccgctg tcctcggtgt ccgactgcca gccgagcatc agcatgtccg ccgcgctctg
     6721 atcgaacagc ggccagtatt gcgccttcgg caccgtcttc aactcgacgc cgatgccgat
     6781 cttggccagc atccccacca ccgcctgggc gatctgctca tcattcacat agcggttgtt
     6841 gggcgccagc atcgtcacct tcagcccctg gccatggcca gcctgttgca gcagttgccg
     6901 ggcttcggcg aggtcgaacc gtggcacgag cgcgggatca tgaccgggat agcccgtcgg
     6961 gctgagctgg gcagctggtg tggcaaaacc gcgcatgatc ttctcgctga tgccgacatt
     7021 gttgattgcc agccgcactg cccggcgcac gcgggggtcg gcaaaggccg gcacccgctg
     7081 ctgattcagt tgcagggtga tgatgcgggt gccggtcatg gtgaccacct cgatggccgg
     7141 ctgccgttgc aggcgcggca gatcggctgg cggtactggc gcgatgaagt cgacatcgcc
     7201 ggccagcagc gccgcgatgc gggtggcatc ctccttgatc ggcgtcagca cgatgcgctc
     7261 gacattgccg ggggattggc gatcccaatg gtcggcaaag cgttgcagtt cgagtcggac
     7321 cccctgctgc cggctcacca gccggaacgg tccactgccg ctgacattgc gggcggcgaa
     7381 ggagtcacca ctcttgacca gtgcctcctt gttgcgcccc tgctcatcct gtccctgata
     7441 gaactgccga tcgagcacaa agatgtaggt ggcgaggttg agcaccagcg gatagggctg
     7501 atgggtgatc agttcgaagg tgtggtcatc gatcactcgc actggcttga agggggcgaa
     7561 cagccccttg aagtccgggc tgcgctgcag ccgggcgaag gtccaggcca catcctggct
     7621 ggtcagcggg cgtccggagt ggaacttgac gcccggccgc agatgaaagc gggtggtgag
     7681 tggatcgatg cgttcccagc gttcggccac tctgggttca aagctgcgat cctgcttcca
     7741 gcgcagcagt ggatcgaaca gcagatgtga gagctggagc gtggcgcctg acaactgctc
     7801 atgcggatcg agcgaatcgg ggtcggcgtc ataggcgacg cgcagcgtgt gctgcgctgc
     7861 tgcggtcgtg cagagcgcaa cgagcagtcc catcagcagc aggcgtgacc agcgggtgcg
     7921 catgggtggg ggactccagt atgggcttga atgcaaatgc agaacgatcc gcaatggggc
     7981 gcgccgtgaa tggtaacttg aaaagcggcg gtgatggagc cccgatgcaa tccgctggcc
     8041 agaggcggca tgaatgaaaa caggcgccaa ggagacccag gcgcctgttc gagaggctgg
     8101 cgtccttgcc ggttggcagg accgctcagt cgtcagctca cagcgggcgc agttgcaccg
     8161 gcagttcaga gtaacccttg atgaagggtg agtagttgcg cactggctcg ccgaccactt
     8221 caatggtctt gaagcgcttc atgatctctt cccaggtgac ccgcagttgc atctcggcca
     8281 ggcggttgcc catgcagcgg tggatgccga agccgaacga caggtggtgg cgcgggttct
     8341 cacggtcgat gatgaactcg taggggcggt cgatcacctc ttcgtcacgg ttgcc
//
LOCUS       DAORCQ010000098         8205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6120_length_8205_cov_5.850307, whole genome shotgun sequence.
ACCESSION   DAORCQ010000098 DAORCQ010000000
VERSION     DAORCQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_8205_cov_5.850307"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4963)
                     /locus_tag="PKI23_09580"
     CDS             complement(<1..4963)
                     /locus_tag="PKI23_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNN37227.1"
                     /translation="MSNNANPSRQAVPKKPAESSVEQNAEAVAIVGMAFRFPGGMEDE
                     QAFWRALQEGRDLVSAIPDERWAVETLQHPKRGEAGRSITFSAGVLSRIDAFDAQFFG
                     ISPREAAWMDPQQRLLLELAWESLENAGQRPSALAGSQCAVYVGIAGLDYGMRALDDL
                     SSITSHFMTGNTLSIVANRLSYVFDLHGPSLAVDTACSSSLVALHHACNSLRSGEAEA
                     ALVGGVNLLLHPFPFVGFTKASMLSAQGRCKVFDAEGDGYVRAEGGAMLFLKPLSRAL
                     ADGDPIKAVILASGTNSDGARNKKGITIPSAEGQSELMRTVLRRSGLAPQQVDFIEAH
                     GTGTAVGDPVETMAIGQVYGQSRPSDRPLPIGSVKSNLGHLEAASGMAGLVKAVLCLQ
                     HRALPPSLHLHTPNPHIDFAGLNLSLVTRNTELTPQSGGRLVAGVNSFGFGGANAHVL
                     LQEPPLRLLPGSHRPEPVAAPPLFLSARTHEALRAMAADYARLLREQPAEAYYDLACS
                     VVRDREWMAQRLALRAEPATALPELLEQFAQAGQVAEVVVETALPEAGGVVFVYSGNG
                     AQWHGMGQRLLGESPRFVELLAEVDSVLQPLSGFSVRAELESEASRLDDTTVAQPLLF
                     AMQVTITQLLRERGVQPLLVLGHSVGEVAAAWCAGALDLEQAARLICARSAAQGLTRG
                     SGRMAAVALSAAEAEACIEALGAALEVEIAGINSAGNVTLSGSLADLETLGAELTAKG
                     VFYRLLDLDYAFHSRRMDPIEQPLRESLAELVPTSSSGVHFVSTVTGEQLSTERLDAG
                     YWWRNVREPVQFARGVERAIALGGGCFIEIGPHAILQRYLGEGLKSANRVGRVLPTLR
                     RDDDGPERIEEAALRAHLLAAQPNLTAYFPAVGRRVALPNYPWQRERHWHPVSSESHG
                     LIQRRRIHPLLGWRLSELEAGWENTLDPRSLPWLADHQVGGAVVFPAAAYAEMALAAA
                     QLWHGKPQTGFEQLDIVAPLVFDGERARTLRFTLNPRDGGFQISSRQRLGEEPWALYA
                     TGRLLDLPSSEAARSMIAPVAVAERTVDHASHYALTAAVGLGYGPAFQGVRELCIDGD
                     LLEAQLSAPAEVADDSRYLLHPALLDLACQSLVDFFQPQIEAGRGVAFLPIKITRLHR
                     HRETSISSLRARLLRHGLRSVLADFELFDADGALVARMEGCRFRAAPLVVPTHGAPMR
                     WRIVPRLRPHPRDLATSQFPAPAQLAETVRQQFLQHEERLARQLWFGETLPLIEALTL
                     SFIHEAFMAVAQPGQEALQAAMQGPVAASAYLRWMAELLIEEGLLIDADGLWRLIPAA
                     ELPPAALIWQTLLRDAPAAVAQLTLLGRVGCQLPRLLRGEASADVLAREVSHSPLAET
                     LLDEDPLHLGSGWAVRKLLELLQAAQPAGRRLRVLELASGPSALPRHFAAALQEERME
                     YLLLHGDEVHCQRQQAEFEKMRNVTVLGIDLADWTLSAQKVALQPFDVVVLRHVVHRA
                     PFPHQALAQARQWLTQGGLLLLAEQHADWSADLLGGLDADWWSEDQAQPRRPLSSLQA
                     PEVWRQLLAEAQFEAIELLNEPTLPAGLNAGSHLLLARRPAQIERRAVEPVPEAERWL
                     LLIDETSAALAHSLRQRLEQQGQQVMMAEAETEAQAIAAGCDHL"
     gene            complement(5119..5700)
                     /locus_tag="PKI23_09585"
     CDS             complement(5119..5700)
                     /locus_tag="PKI23_09585"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNN37228.1"
                     /translation="MIDEDEQHNPWTTLESRQLYDNPWIQVSERQVINPRGGRGIYGV
                     VHFKSRAIGIVPVDDSGHTYLVGQFRYSLGSYSWEIPMGGCPLDEPPLRAAQRELQEE
                     TGLRAARWRHLLTLHTSNSVTDEVGELFLAEGLIEGEAEPEETELLKVWRLPLQEAIG
                     MALDGRITDALSVAALLRLAVEPGLLAMPSAQE"
     gene            5833..5909
                     /locus_tag="PKI23_09590"
     tRNA            5833..5909
                     /locus_tag="PKI23_09590"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5867..5869,aa:Met,seq:cat)
     gene            complement(6173..7996)
                     /gene="ftsH"
                     /locus_tag="PKI23_09595"
     CDS             complement(6173..7996)
                     /gene="ftsH"
                     /locus_tag="PKI23_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNN37229.1"
                     /translation="MDNQSKPRPNSSSHWFALLFTLFFLALYTVFDSSRSSRPVEISY
                     SEFKQAVDGGRVRQVTLSPDRVSGEWSAGQNGRFRAVLPPWQDPQLLPLLEQKGVTIK
                     GQSSESPFWLRAVIAVLPWLLLFVFFLYSSRMMAQRMGGSGLFDFSRSRAHRYDKSRI
                     RVKFDEVAGVDVAKQDLLEVIDYLKHPQKYTRIGARVPHGILMMGPPGSGKTLLAKAT
                     AGEAEVPFFSVSGSEFIEMFVGVGASRVRDMFRQARAEAPALIFIDEIDSIGRVRGTG
                     LGGGNDEREQTLNQILSEMDGFEPEEAVVVLAATNRPDVLDPALLRPGRFDRKITLDL
                     PHSAARLKILQVHTRKTPLASDVDLEQIAARTVGFSGADLQNLVNEAALNAARLDREQ
                     LTAADFDEARDKVMLGSMREELLNEHDRERIAYHEAGHALTAVHVEHADPLTRISIIP
                     RGRALGLTEQLPEEERHHFTEDYFSDRLAILLGGRGAERLVFGVVSSGAADDLKQATR
                     LARKMVAEWGMSELGPLSLPLSEEHPFLGRDIAQPRDFSEASLARIDQEVERLLHTAE
                     MRTEAILQGHRAQLDLLAKALLKQELLNQGEIAAILAAALR"
BASE COUNT         1537 a         2820 c         2471 g         1377 t
ORIGIN      
        1 gcaggtggtc acagccggcc gcgatggcct gcgcctcggt ttccgcctca gccatcatca
       61 cctgctgccc ctgctgctca agccgttgtc gcaacgagtg ggccagcgcc gcagaggttt
      121 catcgatcag caacagccag cgctcggctt cgggcactgg ctcgaccgcc cggcgctcga
      181 tctgcgccgg gcgccgggcc agcagcagat ggctgccagc gttcagccct gcgggcaggg
      241 tcggttcgtt cagaagttcg atcgcctcga actgcgcttc ggccagcagt tgccgccaca
      301 cctccggcgc ctgcagtgac gacagcggcc ggcgcggttg cgcctgatcc tcgctccacc
      361 agtcggcgtc gagcccaccc agcaggtcgg cgctccagtc ggcatgctgc tcggccagca
      421 gcagcaggcc accctgcgtc agccactgcc gcgcctgcgc caatgcctga tgaggaaacg
      481 gcgcacggtg cacgacatgg cgcagcacca cgacatcgaa gggttgcagc gccactttct
      541 gcgccgacag cgtccagtcg gccaggtcga tgcccagcac ggtgacattg cgcatcttct
      601 cgaactcggc ctgctgccgc tggcagtgca cctcatcgcc gtgcagcagc agatactcca
      661 tgcgctcttc ctgcagtgcc gcagcgaaat ggcgcggcag tgcactgggt cccgaggcga
      721 gctccagaac acgcaaccgg cgcccggcag gctgcgccgc ctgcaacagc tccagcagct
      781 tgcgcacggc ccagccgctg ccgagatgga gcggatcctc atcgagcagg gtttcggcca
      841 gcggtgagtg gctcacttcg cgggccagca catcggcaga cgcctcaccc cggagcagcc
      901 gtggcagttg gcagccaacc cgccccagca gcgtcagttg cgcgaccgca gcgggcgcat
      961 cacgcaacag cgtctgccag atcagtgcgg ccggcggcag ttctgcggcc ggaatcaacc
     1021 gccagaggcc atccgcatcg atgagcagcc cctcctcgat cagcagctca gccatccagc
     1081 gcagataggc cgacgcggca accggcccct gcatggcggc ctgcagtgcc tcctgacccg
     1141 gttgcgccac agccatgaag gcctcgtgga tgaacgacag cgtcaatgct tcgatcagcg
     1201 gcagcgtctc accaaaccag agctgccgcg ccagtctctc ttcgtgctgc agaaactgct
     1261 gccgcaccgt ttcggcaagt tgcgccggcg ccggaaactg gctggtcgcc agatcgcgcg
     1321 gatgcgggcg cagcctcggc acgatgcgcc agcgcatcgg tgcgccatgc gtcggcacga
     1381 ccaagggtgc ggcacggaac cggcacccct ccatccgtgc caccagggca ccatcggcat
     1441 cgaacagctc gaaatcggcc agcaccgaac gcagaccgtg acgcagcagc cgcgcccgga
     1501 gcgagctgat cgaagtttcg cgatggcggt gcaaccgggt gatcttgatc ggcagaaagg
     1561 ccacgcctcg tccggcttcg atctgcggct gaaagaaatc gaccaggctc tgacaggcca
     1621 gatcgagcag cgccggatgg agcagatagc gggagtcatc agccacttca gccggcgcac
     1681 tcagctgcgc ctcgagcaga tcgccatcga tgcacagctc ccgcacgccc tgaaaagccg
     1741 ggccgtagcc gagcccgact gcggcggtca gggcgtagtg gctggcatgg tcgaccgtgc
     1801 gctcggccac cgcgaccggg gcgatcatcg accgcgctgc ttcgctgctt ggcagatcga
     1861 gcaggcggcc ggtggcatac agcgcccacg gctcttcgcc aagccgctgg cggctgctga
     1921 tctgaaagcc gccatcgcgt ggattgaggg tgaagcgcag tgtccgcgcc cgctcaccat
     1981 cgaacaccag tggcgcgacg atatcgagct gctcgaaccc cgtttgcggc ttgccatgcc
     2041 acagttgcgc cgccgcgagc gccatctcgg cataggccgc cgccggaaac accacggcgc
     2101 cgcccacctg atggtcggcc agccagggca aggaccgcgg atcgagcgtg ttctcccagc
     2161 cggcctccag ctcggagagg cgccagccca gcagcggatg gatgcgtcgc cgctggatca
     2221 ggccatggct ttcgctggaa accggatgcc agtgccgctc acgctgccat ggatagttgg
     2281 gcagggcgac ccgccgcccc actgccggaa agtaggccgt aaggttcggc tgcgctgcaa
     2341 gcaggtgggc acgcagggcc gcctcttcga tccgctccgg gccgtcgtcg tcacggcgca
     2401 gtgtcggcag cacccgtccc acccggttcg ccgacttgag cccttcaccg aggtaacgct
     2461 gcaggatggc atgggggccg atttcgatga agcagcctcc acccagagca atggcacgct
     2521 cgacacccct ggcgaactgc accggctcac gcacgttgcg ccaccagtag ccggcatcga
     2581 gccgttctgt gctcaactgt tcgccggtca ccgtcgagac aaagtgcaca ccgctcgaac
     2641 tggtcggtac cagttctgcc aggctctcgc gcaacggctg ctcgatcggg tccattcgcc
     2701 gactgtggaa ggcgtaatcg aggtcgagca gccgataaaa gacccccttg gctgtcaact
     2761 cggcaccgag ggtttcaagg tcggccaggc tgcccgagag cgtgacattg ccggcactgt
     2821 tgatgccggc aatctccact tccagagcag cgccaagcgc ctcgatgcag gcttcggcct
     2881 ccgcagccga cagcgcgacg gccgccatgc gcccactgcc cctggtcagc ccctgcgcgg
     2941 cgctgcgggc acagatcagc cgcgcagcct gctcgaggtc gagcgcgccg gcacaccagg
     3001 ccgccgcgac ctcaccgacg ctgtgtccca gcaccagcag cggctgcacc ccacgctcgc
     3061 gcagcaactg ggtgatggtc acctgcatcg caaacagcag tggctgggcc acggtggtgt
     3121 cgtcgagtcg cgaagcctcc gactccagtt cggcccgcac cgagaaaccg ctcagcggtt
     3181 gcagaaccga atccacctcg gccagcagtt cgacaaaacg gggagattcg cccagcagtc
     3241 gctgccccat gccatgccac tgcgcgccat tgccggagta gacaaaaacc actccccctg
     3301 cttcgggcag agcggtctcg accacgactt cggccacctg cccggcctgc gcaaactgct
     3361 ccagcagctc cggcagtgcc gtggcgggtt cggcccgcag cgcaaggcgc tgcgccatcc
     3421 actcacggtc ccgcacgacc gaacaggcca ggtcgtagta ggcttcagcg ggctgttcgc
     3481 gcagcagccg ggcgtagtcc gccgccatcg cccgcagcgc ctcgtgcgtg cgggccgaca
     3541 gaaacagcgg cggcgctgcc accggctcag gtcgatgact gcccggcagc agccgcagcg
     3601 gtggctcctg cagcaacaca tgggcattgg cgccgccgaa gccgaatgaa ttgacacccg
     3661 ccaccagccg cccgccggac tggggcgtca gttcggtgtt ccgggtgacc agactcaggt
     3721 tcagcccggc aaagtcgata tggggattgg gcgtgtgcag atgcagcgag gggggcaagg
     3781 cgcggtgttg caggcagagc acggccttca ccagacccgc catgccggaa gccgcctcca
     3841 gatggcccag gttgctcttg accgatccga tcggcagcgg ccgatcgctt gggcgcgact
     3901 ggccatagac ctgaccgatc gccatcgtct cgaccggatc acccaccgca gtgccggtgc
     3961 catgcgcctc gatgaaatcg acctgttgcg gcgccaggcc gctgcggcgc aacacagtgc
     4021 gcatcagctc gctctgaccc tcggcgctgg gaatggtgat gcccttcttg ttgcgggcgc
     4081 catcgctgtt ggtgccgctg gccaggatca ccgccttgat cggatcacca tcggccagtg
     4141 cccgcgacaa cggcttgaga aacagcatcg cgccgccttc ggcacggaca tagccatccc
     4201 cttcggcatc gaaaaccttg cagcgcccct gcgccgagag catcgaagcc ttggtgaagc
     4261 ccacgaatgg aaaaggatgc agcagcagat tgaccccacc caccagtgcc gcttcggcct
     4321 cgccgctgcg cagactgttg caggcatggt gcagcgccac cagcgatgat gagcaggcgg
     4381 tgtcgacagc cagtgaaggg ccatgcaggt cgaacacata ggagagccgg ttggcgacga
     4441 tgctcagcgt gttgccggtc atgaagtggc tggtgatgct ggaaaggtca tccagcgccc
     4501 gcatgccgta atcgagcccg gcgatgccga catagaccgc gcactggctg ccggccagcg
     4561 ctgaaggtcg ctgcccggcg ttttccaaag actcccaggc cagctccagc aacagacgct
     4621 gctgcggatc catccaggcc gcttcgcggg gcgaaatgcc gaagaattgc gcatcgaacg
     4681 catcgatgcg cgacagcaca ccggccgaga aggtgatgct gcgccccgcc tcgccgcgct
     4741 tcgggtgttg cagcgtctca accgcccaac gctcatcggg aatggcgctg accagatccc
     4801 gcccctcctg cagcgctcgc cagaaggcct gctcatcctc catgccaccc gggaaacgga
     4861 aggccatgcc gacgatggcc acggcttcgg cattctgctc gacggacgat tcagcgggct
     4921 ttttcggcac tgcctggcga cttggatttg cgttattgct caaaaaagtg cggggatcca
     4981 tagggccgtg atgatgctcc tcaaggcgcg cctgcatcag tacggcgcca agccatgcga
     5041 tgcaaaatga cgaaaaaaac ttattctata aaaatcaaaa tgatataaat atccttaccc
     5101 tatatgacaa ttgtaacttt attcttgcgc cgatggcatc gcaagcaatc cgggttccac
     5161 cgccagacgc agcagcgctg ccacgctgag cgcatcggtg atgcgcccat ccagcgccat
     5221 gccgatcgcc tcttgcagcg gcaggcgcca gaccttcaac agttcggtct cctcaggttc
     5281 ggcctcgccc tcgatcagtc cttcggccag gaacagctcg cccacctcat cggtgaccga
     5341 gttggaggtg tgcagcgtca gcagatggcg ccagcgcgct gcccgcaggc cggtctcctc
     5401 ctgcagttcg cgctgggctg cccgcagcgg cggctcatcg agcggacagc cacccatcgg
     5461 aatctcccag gagtagctgc caaggctgta gcggaactgg ccgaccaggt aggtgtggcc
     5521 actgtcatcg accggcacga tgccgatggc ccggctcttg aagtgcacca ccccatagat
     5581 gccgcgcccg ccgcgcggat tgatcacctg ccgctcgctc acctgaatcc aggggttgtc
     5641 atacaactgc cgactctcca gcgtggtcca tggattgtgc tgctcatctt catcaatcac
     5701 cgcaactctc ctgtggtctg tgcgctcttg cgccaccctg tgctcgatca tctgttcacc
     5761 aaagacagca gcttccctgc ttgacacaat ggccctgatc atcaagaatg cccgacctat
     5821 ttttgaccgt tcggctatgt agctcagccg gttagagcac agcactcata atgctggggt
     5881 cggtggttcg agtccaccca tagccaccat ctcctctcaa acacaaaagc cagcgcaacg
     5941 ctggcttttt gttcttgctg aatcaatcgg atgcgagagc gtgacctttg cgttcaccct
     6001 tctcgccgaa aagcgaaatg agctccagcg gaagcacgct gcggccgctc ccaccgtcag
     6061 ggtgttgcgc aggtgcgaaa tgggcggcgg tgtccagcag cgcagactga cgccgacgca
     6121 ccaacggcaa gcgttccgcg acaccggccg caagcgcttt ctcttcatgc ggctaccgca
     6181 gtgccgccgc gagaatcgca gcaatttcac cctgattgag cagctcctgt ttcagcaatg
     6241 ccttggccag caggtcgagt tgcgcacggt gcccctgcaa gatcgcctcg gtccgcatct
     6301 cggcagtgtg cagcagccgc tccacctcct gatcgatgcg tgccaggctg gcttcactga
     6361 agtcccgagg ctgagcgatg tcgcggccga gaaacggatg ctcctcgctc agcggcaggc
     6421 tgagcggtcc cagttcgctc atgccccact ccgccaccat tttgcgcgcc aaccgtgtgg
     6481 cctgcttcag gtcatcggcg gccccggagc tgaccacgcc aaagaccaac cgttcggcgc
     6541 cacgtccacc cagcaggatc gccagccggt cgctgaagta atcctcggtg aagtggtgac
     6601 gctcctcttc gggaagctgt tcggtcaggc ccagcgcgcg gccgcgcggg atgatcgaaa
     6661 tgcgtgtcaa cgggtcggcg tgctcgacat ggacggcggt cagggcatgg cctgcctcat
     6721 ggtaggcgat ccgttcacgg tcatgttcgt tcagcagctc ctcgcgcatc gagccgagca
     6781 tcaccttgtc ccgcgcctcg tcgaaatcgg cggccgtgag ttgctcccgg tccagtcggg
     6841 cggcattgag cgctgcctca ttcaccagat tctgcaggtc ggcgcccgaa aatccgaccg
     6901 tgcgggcggc gatctgctca agatcgacat cgctggcaag cggcgtcttg cgcgtgtgca
     6961 cctgcaggat cttgagccgt gccgccgagt gcggcagatc gagggtgatc ttgcggtcga
     7021 atcgccctgg tcgcagcaag gcgggatcaa gcacatcggg tcggttggtg gccgccagca
     7081 ccaccacggc ctcttccggc tcgaatccgt ccatctcgga gagaatctga ttgagcgtct
     7141 gctcgcgctc atcgttcccc ccgcccaggc cagtaccgcg cacccgtcca atcgaatcga
     7201 tctcgtcaat gaaaatcaac gctggcgctt cggcgcgcgc ctgtcggaac atgtcccgca
     7261 cccgcgaggc gcccacaccc acgaacatct cgatgaactc ggagccactg acgctgaaaa
     7321 acggcacttc ggcttcgccc gcagtggcct tggcaagcag cgtcttgcca gaaccgggcg
     7381 gccccatcat caaaatgcca tgcggcactc gggcgccgat cctggtgtac ttttgggggt
     7441 gcttcagata gtcgatcacc tcaagcagat cctgcttggc cacatcgaca ccggccacct
     7501 catcgaactt cacccggatg cggcttttgt catagcggtg agcacgtgag cgagaaaaat
     7561 cgaacaagcc gctgcccccc atgcgctgcg ccatcatcct gctgctgtag agaaaaaaga
     7621 caaaaagcag cagccacggc aacacggcaa tcaccgccct cagccagaat ggactttcag
     7681 aggattggcc cttgatcgtc acccccttct gctccagcag cggcagcagt tgtggatcct
     7741 gccaaggcgg caagaccgcc ctgaaccgcc cgttttgccc cgcgctccat tcaccactga
     7801 cacggtctgg actcagtgtg acctggcgaa ctcgtccgcc atccaccgcc tgcttgaact
     7861 cgctgtagct gatttcgacc ggtcggctcg aacggctgga atcaaaaacc gtatacaaag
     7921 ccagaaaaaa cagcgtaaac agcagtgcaa accagtggct cgaagagttt ggtctgggct
     7981 tggattggtt gtccatgtcg ctctgtggcc agtgaaaagt ggaatgtgca tcctgctcca
     8041 tgcgtcgata tgcgggatcg attgccgtcc cggccgagaa tagcaagacc acaacggaaa
     8101 aagggctgcc aagcagccct ttttctctcg atcaaccgtc atacgcgaat cgatcaaccc
     8161 gcgaatgggc acttctccac ggaggccgag ccgctctggc tgccc
//
LOCUS       DAORCQ010000099         8065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6254_length_8065_cov_5.033333, whole genome shotgun sequence.
ACCESSION   DAORCQ010000099 DAORCQ010000000
VERSION     DAORCQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6254_length_8065_cov_5.033333"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..766)
                     /locus_tag="PKI23_09600"
     CDS             complement(89..766)
                     /locus_tag="PKI23_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNN37230.1"
                     /translation="MSSKRRNPQNADAIHRRLKEMILNFEFYPGTRLTEGELSERFGV
                     SRTPIREAVQRLEMERLMTVRPKQGCFVRELDIDEINDYYQVRMALERLMLQLVIERM
                     PRQALLDLAAEWAPSQIPTDVIDVETMALRDESFHLALATGCGNRVLVQKLREINEHI
                     HIIRRLDFTDVGRIEATYREHHALCQCLLDGRLDEALALLQSHIERSQEFSQGLTLRR
                     LAEMRTG"
     gene            1038..1820
                     /locus_tag="PKI23_09605"
     CDS             1038..1820
                     /locus_tag="PKI23_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HNN37231.1"
                     /translation="MNQPLPNTAIERHPSRQLRGLLAGLCCTLTTACLADGGGFSTTN
                     FQYLRGDNYELGDQQRSIITVEHANGWAYGDNFFFVDVTNPDRNGNQTGTGYYAEFSP
                     RLSFGKMFGADLSSGIIRDYLITTTLELPEEPVDKTYLYGLAIDLDLPGFAFFQTNFY
                     IRNSQAPGVDTGHQITLAWLYPFHTGSLNWVFEGFLDYAFGEDPLEDNLLTAPRLLLD
                     LGALAGSPSKIYAGVEYQYWDNKFGIDGVTESVAQAMLKWVF"
     gene            1833..3620
                     /gene="atzF"
                     /locus_tag="PKI23_09610"
     CDS             1833..3620
                     /gene="atzF"
                     /locus_tag="PKI23_09610"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HNN37232.1"
                     /translation="MTTVATTMNLNALRSRYAKGLRVSQLVESLLDPLARSRVQGVWI
                     HCLDDDALLARARELDAAGPADKPLFGIPFAVKDNIDVAGLPTSNGCRATVDWPAQSA
                     PVVQRLLDAGALLLGKSNMDQFAMGLVGTRSPYGLCPNPFNPDYIAGGSSSGSAVAVA
                     RGLASFALGTDTAGSGRVPAALNHLVGLKPTPGRVSSRGLRPSCRSLDCISLFAHTIA
                     DSWELLQLIEGFDAADPYSSQPQAYPCALRQDAARIGVAEPDLLDFGGDPASQALYSR
                     AVQAVTQSGLQRVETKIEPLLEAGRLLYDGPWVAERHVAVGELDRNAMQPPIAAILDQ
                     AADYSAADLFRAQHRLAQLRREADLLWQQVEALLLPTLPRPYRIAEIKAEPLRCNSHL
                     GLYTNFVNLLGLCAIAIPVGLLPSGVPYGVSLIAPAWSEARLCRIAEQLLAAIETRSL
                     AAAQGCHADRSEQIIAVCGAHMSGLPLNWQLLELGGHLLAESSTAPCYRMRLLEGSGP
                     PRPCLLRSDSDGAALPLELWSLPGSQIGKLLAQVPAPLALGLIELADGRSVHGFIGQA
                     GAVEHGRDITALGGWRNHLASLSARKPDG"
     gene            3685..5250
                     /locus_tag="PKI23_09615"
     CDS             3685..5250
                     /locus_tag="PKI23_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37233.1"
                     /translation="MQTQPKLWTSGDWNAFFGFGTNILLNLLVLTGLLTLVLKMPSDL
                     VFGRILPATGLMLFLSTLYYAWLAWRLARQSGRDDVCALPSGISVPHMFVVVFVIMLP
                     IATATGDPIRGWEAGLTWVFVQSFVLMAGGFVAPWIRRITPRAALLGALAGVSIAFIS
                     MRPALEMFMTPAIGLICFAIIVLNWFGGVRYFKGAPAGLVAIAVGSLIAWGSTAFGLN
                     YGGMSLAKLGASFSNFGFSLPLPVIGHVFSGFEFIGIILVTAIPFGIYDLVEAMDNVE
                     SAAAGGDHFPTTRVLTADGVVSLIGCLLGNPFINAVYIGHPGWKAIGGRIGYSAATGI
                     MVLILSWFGIIAVLMALIPVVAIAPILLYIGMLIGAQAFQETPKNHAPVVILALVPQV
                     AAWGKLQIDSALAAAGTSAASVGLDKLGQGGVLYEGLSVLGGGAILGSLILAAIGACI
                     IDRHFIKAASFALVGSLLTFFGFMHGEAIGIGHHPAVAVGYLLMAALLYGCAQSEKAT
                     QPALQPAIADLAD"
     gene            5423..5776
                     /locus_tag="PKI23_09620"
     CDS             5423..5776
                     /locus_tag="PKI23_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNN37234.1"
                     /translation="MYRKALFTTLLGTALLTNGVEAGRGNGSPNDPRIDATKVDSQQT
                     LLTIQGDHFGSSMPSVVMGRWQLKVKTHSNQEIVAELPKGVSGSSYRMQVISGAPANA
                     SSEPFFTTLFTASNP"
     gene            complement(5809..6759)
                     /gene="arcC"
                     /locus_tag="PKI23_09625"
     CDS             complement(5809..6759)
                     /gene="arcC"
                     /locus_tag="PKI23_09625"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNN37235.1"
                     /translation="MSETAVIALGGNALISDAGHEAIPDQYQRVCRAAPHLVDLIEQG
                     WRVVISHGNGPQVGFILRRSELAEAEVDPVPLDYAVADTQGAIGCMFVKALTNELTRR
                     GLDRPVIALVTHTLVDVDDPAFARPDKPVGAFLSEATARARQQMLGWSIMEDAGRGWR
                     RTVPSPQPRRILESATIHQLLEQGAVVIAAGGGGIPVSIGADGLLSGVEAVVDKDRAS
                     SLLAQELAARMLIIPTSVAQVAIRFGTPAQQWLDRLTVEQARDYLAAGEFGAGSMAPK
                     VAALADFVACTPGAVGAIGLPEQIAAIVDGRAGTRITASG"
     gene            complement(6756..>8065)
                     /locus_tag="PKI23_09630"
     CDS             complement(6756..>8065)
                     /locus_tag="PKI23_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HNN37236.1"
                     /translation="ADADLGWKLAALIGDPRIDEANRTAYGRYLAARPRLVDLQRARD
                     AIAALGGGERRILHAGPPIDWAQMCGPQQGAIAGALLYEGWADSLEAAARMAAAGEVA
                     LEPCHDHGAVGPMAGVISPSMPLWVVENGDRGNRAYCNLNEGLGKVLRFGANTPEVIA
                     RLRWLGSEFFTTLRVAVRALEDGELKPLMAQALQMGDELHNRNLAASGLLFKRLALAL
                     LGSDLDSGAIQRTLGFIAGNDHFFLNLSMAACKAMLDAAHGVPGSSLVTVMARNGVNF
                     GIRLSGTGAQWFEAPANPVQGLYFAGYGVEDAAADLGDSAITETAGLGGFAMAAAPAI
                     VQFVGGTAADASANSRRMLEITLGSNPAFSLPPLDFAGTPAGIDARLVVDSGILPVIN
                     TGIAHRQAGVGQIGAGITTAPLACFNEAIAALCQMLDRNGEAA"
BASE COUNT         1352 a         2762 c         2529 g         1422 t
ORIGIN      
        1 gccgccaccg cgcagtcgtt gtcgcaggct tatgagttcc tgcgccggct ggagttgcgg
       61 cgcaacggcc ggccctaggg cacgccgttc aacccgtgcg catctcggca agccgccgca
      121 gcgtcagccc ctgcgaaaac tcctgactgc gctcgatgtg gctctgcaac agcgcgagtg
      181 cctcgtcgag ccggccgtcg agcaggcact ggcagagggc gtggtgctcc cggtaggtgg
      241 cctcgatgcg cccgacatcg gtgaaatcga gccggcggat gatgtggatg tgctcgttga
      301 tctcgcgcag cttctgcacc agcacccggt tgccgcagcc ggtggccagc gccagatgga
      361 aggactcatc gcgcagcgcc atcgtctcca cgtcgatcac atcggtcggg atctggctcg
      421 gcgcccactc ggcagccaga tcgagcagcg cctggcgcgg catccgctcg atcaccagtt
      481 gcagcatcag ccgctccagc gccatgcgca cctggtagta gtcgttgatc tcgtcgatgt
      541 cgagttcccg gacaaaacag ccctgcttcg gccgcaccgt catcaaccgc tccatctcca
      601 gccgctgcac cgcttcacgg atcggcgtgc ggctgacgcc aaagcgctcg ctcagctcgc
      661 cctcggtcag ccgggtgccg gggtagaact cgaagttgag gatcatctct ttcaatcgac
      721 gatggatggc gtcggcattc tgcgggtttc tgcgcttgct ggacatcaca ctaccgtaag
      781 ttgtgaatct ggcgctccgg cctggaaatg tctggctggt gttctgcact tttgtagttc
      841 atcacaccgg aacatgtcca gccttggggc agattctttt tggaatcatc ggcagaacaa
      901 cagaaactga ctcctatcgt tttgatttta atcaatattt atctaactct agacacttgc
      961 aacaaactgg cacgctttgc gcatcaaatg tatacaacaa taaattcatt gttgtttgca
     1021 acacgccgga gagatacatg aaccagccgc tgcccaacac cgcaatcgaa cgccacccgt
     1081 caaggcaact ccgcggcctg ctggccggac tctgctgcac cctcaccacg gcctgcctgg
     1141 ccgacggtgg cggcttcagc accaccaact tccagtacct gcgtggcgac aactatgaac
     1201 tgggcgacca gcagcgctcg atcatcaccg tcgaacatgc caatggctgg gcctatggtg
     1261 acaacttctt cttcgtcgat gtcaccaatc ccgatcgcaa cggcaatcag accggtaccg
     1321 gctattatgc cgagttctcg ccgcgtctga gtttcggcaa gatgttcggt gccgacctct
     1381 ccagcggcat catcagggat tacctgatca ccacgacgct ggaactgccc gaggagccgg
     1441 tcgacaagac ctacctctat ggcttggcga tcgacctcga cctgcccggt ttcgccttct
     1501 ttcagaccaa cttttacatc cgcaacagtc aggcgccggg cgtcgacacc ggccatcaga
     1561 tcactctggc ctggctctat ccgttccaca ccggcagcct caactgggta ttcgagggct
     1621 ttctcgacta cgcctttggc gaggatccgc tggaagacaa cctgctgacg gcaccccggc
     1681 tgctgctcga tctgggtgcg ctggccggct caccgagcaa gatctacgct ggtgtcgaat
     1741 accagtactg ggacaacaag ttcggcatcg acggtgtcac cgaaagcgtt gcccaggcca
     1801 tgctgaagtg ggtcttctag gccaggatga cgatgaccac tgtggccacc accatgaatc
     1861 tgaacgcact gcgcagccgc tatgccaagg gactgcgggt cagccaactg gtcgagtcgc
     1921 tgctcgatcc gttggcgcgc agcagagtcc agggggtgtg gatccactgc ctcgatgacg
     1981 acgccctgct ggcgcgcgca cgcgaactgg atgcggcagg tccggccgac aagccgctgt
     2041 tcggtattcc gttcgcggtc aaggacaaca tcgacgtggc cggcctgccg accagcaatg
     2101 gttgtcgcgc cactgtcgac tggccagcgc agtcggcgcc ggtggtgcag cgactgctcg
     2161 atgccggcgc gctgctgctg ggcaagagca acatggacca attcgcgatg gggctggtcg
     2221 gcacccgttc gccctatggc ctctgcccca atccattcaa ccccgactac attgccggcg
     2281 gctccagttc gggctcggcc gtcgccgtgg cccgcgggct ggccagcttc gcactgggaa
     2341 ccgacaccgc cggttcggga cgggtgccgg ccgcgctcaa ccatctggtc ggactaaaac
     2401 cgacgccggg tcgggtcagc agccgtgggc tgcgcccctc ctgccgttcg ctggactgca
     2461 tctcgctctt cgcccacacc attgccgaca gttgggagct gttgcagttg atcgaaggat
     2521 tcgacgccgc agacccctac tccagccagc cgcaggccta tccttgcgcc ctgcggcagg
     2581 atgcagcgcg aatcggcgtg gcggagccgg atctgctcga cttcggcggc gatccggcca
     2641 gccaggcgct ctatagccgg gcggtgcagg ccgtgacgca aagcggactg caacgggtcg
     2701 agacgaaaat cgaaccactg ctcgaagccg gccggctgct ctacgacggc ccttgggtgg
     2761 cggagcgcca tgtcgccgtg ggcgaactgg accgtaatgc aatgcaaccg cccatcgctg
     2821 ccattctcga tcaggcggct gactacagcg ccgccgacct gttccgggcg cagcaccgtc
     2881 tggcccagtt gcggcgcgag gccgacctgt tgtggcagca ggtcgaggcg ctgctgctgc
     2941 caaccctgcc gaggccctac cgcatcgccg agatcaaggc cgagccgctg cgctgcaaca
     3001 gccacctcgg cctctacacc aacttcgtca acctgctcgg cctctgtgcc atcgccattc
     3061 cggtcggact cctgcccagc ggcgtgccct atggcgtcag cctgatcgcg cccgcctgga
     3121 gcgaagcccg gctgtgccgg atcgccgagc agttgctggc ggccatcgaa acgcgctccc
     3181 tggcagcggc gcaaggctgc cacgccgacc gcagcgagca gatcatcgcg gtctgcggcg
     3241 cccacatgag cggcctgccg ctgaactggc aactgctgga gctgggcgga catctgctgg
     3301 ccgagagcag caccgcgccc tgctatcgaa tgcggctgct cgaaggcagc ggtccgcccc
     3361 gcccctgcct gctgcgcagt gacagcgatg gggcggcact gccgctggag ctctggagcc
     3421 tgccgggcag ccagatcggc aagctgctgg cgcaggtgcc cgccccgctg gcgctggggc
     3481 tgatcgagct ggccgacggc cgcagcgtcc atggtttcat cggccaggcc ggcgccgtcg
     3541 agcatggccg cgacatcacc gccctgggcg gctggcgcaa ccatctggcc agcctgtcgg
     3601 cccgcaaacc cgacggatga atttcagcaa cccaaacgaa ccgatcaccg acccaaccat
     3661 cgaccaaccc gaggagctgt ggccatgcaa acccaaccca agctgtggac ttcaggtgac
     3721 tggaatgcct tcttcggttt cgggaccaac atcctgctca acctgctggt gttgaccggt
     3781 ctgctgacgc tggtgctgaa gatgccgagc gatctggtgt tcggccgcat cctgcccgcg
     3841 accggactga tgctgttcct ctccaccctc tactacgcct ggctggcctg gcggctggcc
     3901 agacagagcg gccgcgatga tgtctgtgcg ctgccgtcgg gcatcagcgt gccgcacatg
     3961 ttcgtggtgg tgttcgtcat catgctgccg atcgccaccg cgactggcga tccgatccgc
     4021 ggctgggaag ctggcctcac ctgggtcttc gtgcagagct tcgtgctgat ggccggtggc
     4081 ttcgtggcac catggatccg caggatcacc ccgcgcgcgg cgctgctggg cgcactggcc
     4141 ggcgtctcga tcgccttcat ctcgatgcgc ccggcgctgg agatgttcat gaccccggcc
     4201 atcggtctga tctgcttcgc catcatcgtc ctcaactggt ttggcggcgt gcgctacttc
     4261 aagggtgctc ctgccggact ggtcgccatc gccgtcggca gtctgatcgc ctggggctcg
     4321 accgcgttcg gcctgaacta tggtggcatg agcctcgcca agctcggcgc gtcattcagc
     4381 aatttcggct tttcgctgcc gctgccggtg atcggccatg tcttcagcgg cttcgagttc
     4441 atcggcatca tcctggtcac cgccattccg ttcggcatct atgacctggt cgaggcgatg
     4501 gataatgtcg agagcgcagc cgccggcggc gaccactttc ccaccacccg cgtgctgacc
     4561 gccgatggcg tggtgagtct gatcggctgc ctgctcggca atcctttcat caatgcggtc
     4621 tacatcggcc atccgggctg gaaggcgatc ggtggccgca tcggctactc ggccgccacc
     4681 ggcatcatgg tgctgatact ctcctggttc ggcatcatcg cggtgttgat ggcgctgatt
     4741 ccggtggtgg cgatcgcccc gatcctgctc tacatcggca tgttgatcgg tgcccaggcg
     4801 ttccaggaga ccccgaagaa ccatgcgcca gtggtgatcc tggcgctggt gccgcaggtg
     4861 gccgcctggg gcaagttgca gatcgacagc gcactggccg cggccggcac cagcgccgcc
     4921 agcgtcggac tcgacaagct gggccaaggc ggcgtgctct atgagggatt gagcgtgctg
     4981 ggcggcggcg ccatcctcgg cagcttgatt ctggccgcga tcggtgcctg catcatcgac
     5041 cgccacttca tcaaggccgc cagcttcgcc ctggtcggca gcctgctcac cttcttcggc
     5101 ttcatgcatg gcgaggcaat cggcatcggc caccatccgg ctgtggctgt cggttacctg
     5161 ctgatggcgg cgctgctcta tggctgcgcg cagagcgaga aggccaccca gccggcgctg
     5221 caaccggcca tagccgatct ggccgattga gcccagcggc acagcgtcgt ctgcctgatt
     5281 cccgaccctg aaaaggccaa cgccatcgag tccgactgac cacaacaggg aggaggtgtg
     5341 tccaaaacgg ttccgtcatg tcgaaagcag aagatcggaa gttcgtcctc tcaccaccat
     5401 tcaagttcac aaggatattc tcatgtacag gaaagccctc tttaccaccc tgcttggcac
     5461 cgctctgctg accaacggcg tcgaggccgg acgtggcaac ggcagcccca atgaccccag
     5521 gatcgacgcg acgaaggttg acagccagca gacgctgctg accatccagg gcgaccactt
     5581 cggcagctcc atgccgagcg tggtgatggg ccggtggcag ttgaaggtca agacacactc
     5641 caaccaggag atcgtcgctg agctgccaaa gggcgtcagc ggcagctcct accggatgca
     5701 ggtgatctcg ggcgcgccgg ccaatgccag ctcggaaccc ttcttcacga cgctcttcac
     5761 ggccagcaac ccatgagcgg ctgacgccac gctcccaact ggtggcgctc agccgctggc
     5821 ggtgatgcgg gtgccggccc ggccgtcgac gatcgccgcg atctgctccg gcaggccgat
     5881 ggcgccgacc gcccccgggg tgcaggcaac gaagtcggcc agcgccgcca ccttcggcgc
     5941 catgctgccg gcaccgaatt cgccggcagc caggtagtcg cgggcctgct cgaccgtgag
     6001 ccgatcgagc cactgctggg ccggtgtgcc gaagcggatc gccacctggg cgacactggt
     6061 ggggatgatc agcatccgtg cggccagctc ctgcgccagc aggctgctgg cgcggtcctt
     6121 gtcgaccacc gcctcgacgc cgctcagcag gccatccgca ccgatgctga ccggaatgcc
     6181 gccaccgccg gccgcgatga ccaccgcccc ctgttccagc aactggtgga tggtcgcgct
     6241 ctcaaggatg cgtcgcggct gtggtgacgg caccgtccgc cgccagcccc gtccggcatc
     6301 ctccatgatg ctccagccca gcatctgctg tctggcgcgg gcggtggcct cgctcagaaa
     6361 ggcgccgacc ggtttgtccg gccgggcaaa ggctggatcg tcgacatcga ccagcgtatg
     6421 ggtcaccagg gcgatgaccg ggcggtcgag gccacggcgg gtcagttcgt tggtcagcgc
     6481 cttgacgaac atgcagccga tcgccccctg ggtgtcggcc acggcatagt cgagcggcac
     6541 cggatcgacc tcggcctccg ccagctccga gcgccgcagg atgaagccga cctgcgggcc
     6601 attgccatga gaaatcacca ctctccagcc ctgttcgatc aggtcgacca gatgaggcgc
     6661 ggcacgacag acccgctgat actgatcggg aatcgcctca tgtccggcat cgctgatcag
     6721 cgcgttgcca cccagtgcga tgacggccgt ttcgctcatg ccgcctcccc attccggtcg
     6781 agcatctggc agagcgcggc aatggcctcg ttgaagcagg ccagcggcgc agtggtgatg
     6841 ccggcaccga tctggccgac gccggcctga cggtgggcaa tgccggtgtt gatgaccggc
     6901 aggatgccgg agtcgaccac cagccgggca tcgatgccgg ccggcgtgcc ggcgaaatcg
     6961 agcggtggca ggctgaaggc cgggttgctg ccgagtgtga tctccagcat ccgccggctg
     7021 ttcgcgctgg catcggccgc agtgccgccg acgaactgca cgatcgccgg cgcggcggcc
     7081 atcgcaaagc cgccgagccc ggcggtctcg gtgatggcgg agtcgccgag gtcggcggcg
     7141 gcatcctcga cgccatagcc ggcgaagtag agcccctgca ccggattggc cggcgcctcg
     7201 aaccactgtg cgccggtgcc gctgagacga atgccgaagt tgacgccatt gcgcgccatg
     7261 accgtcacca ggctgctgcc gggcacgcca tgcgccgcat cgagcatcgc cttgcaggcc
     7321 gccatcgaca ggttgagaaa gaagtggtcg ttgccggcga tgaagccgag cgtgcgctga
     7381 atcgcaccgc tgtcgaggtc gcttccgagc agcgccagcg ccagccgctt gaacagcagg
     7441 ccggaggccg ccagattgcg gttatgcagc tcatcgccca tctgcaacgc ctgcgccatc
     7501 agcggcttca gctcgccatc ctccagcgcc cgcaccgcca cccgcagcgt ggtgaagaac
     7561 tcgctgccga gccagcgcag ccgcgcgatc acctccggcg tgttggcgcc aaagcgcagc
     7621 accttgccga gcccttcgtt gaggttgcag taagcgcggt tgccgcggtc gccattctcc
     7681 accacccaga gtggcatcga cggactgatc acaccggcca tcggccccac tgcgccatgg
     7741 tcgtggcagg gttcgagggc cacctcgccg gcggcggcca tgcgtgccgc cgcctccagc
     7801 gaatcggccc agccttcgta gagcagcgcc ccggcgatcg ccccctgttg cggaccgcac
     7861 atctgcgccc agtcgatcgg cggaccggcg tggaggatgc gccgctcgcc gccgcccagc
     7921 gcggcaatcg catcgcgtgc ccgttgcaga tcgaccagcc gcggccgtgc cgcaagatag
     7981 cggccatagg cggtgcggtt ggcctcatcg atgcgcggat cgccgatcag cgcggccagc
     8041 ttccagccga ggtcggcatc ggcac
//
LOCUS       DAORCQ010000100         8054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6263_length_8054_cov_5.577197, whole genome shotgun sequence.
ACCESSION   DAORCQ010000100 DAORCQ010000000
VERSION     DAORCQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6263_length_8054_cov_5.577197"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1385
                     /gene="serS"
                     /locus_tag="PKI23_09635"
     CDS             99..1385
                     /gene="serS"
                     /locus_tag="PKI23_09635"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNN37237.1"
                     /translation="MLDPKRFRTELDELAARLASRNFTLDVEQIRRLEARRKALQVDT
                     EALQNDRNRQAKEIGQAKRDGRDVAALLAAGEDLGERLTALQQELSAVQEALEQILLG
                     VPNLPHASVPIGRGEADNQEQSRWGEPRRFDFTPLDHVALGAGAGMDFEAGAKLTGAR
                     FVVLEGGLARLHRALIQFMLDLHTGEHGYRELYVPYIVNADSLRGTGQLPKFEADLFK
                     LVGEENYYLIPTAEVPVTNLARDRIVEAEQLPLKWVCHTPCFRSEAGSHGRDTRGMIR
                     QHQFEKVELVQLVAAEASHDALEVLTGHAEAVLQRLELPYRKMVLCTGDIGFSAAKTY
                     DLEVWLPSQNCYREISSCSNFEDFQTRRMQARQRHPVSRKPELLHSLNGSGLAVGRTL
                     LALMENFQQADGSIVLPDALRPYLGGMARLDLERAG"
     gene            1393..2799
                     /gene="cysG"
                     /locus_tag="PKI23_09640"
     CDS             1393..2799
                     /gene="cysG"
                     /locus_tag="PKI23_09640"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HNN37238.1"
                     /translation="MDYLPICIDLRGRHCLLVGGGEVARRKAETLLRAGATLRLVAPA
                     IDPGLGELVRQRGGELRLRAFEPRDVDDVWLAVAATDDEAVNRQVRDAARQRGVLVNV
                     VDQPALCDFIFPSIIDRDPLLIAVSSGGRAPLLARLLRARLETTIPAGYGRLAAWMGR
                     FRPQVKARIAGGTARRRFWERVLEGQIGETFLAGREAEAERLLTASLTAGTVDEVGEV
                     YLVGAGPGDPDLLTFRALRLMQKADVVLYDRLVAAPIVDLVRKEAERIHVGKERDRHT
                     LPQSRINQLLIDLARSGKRVLRLKGGDPFIFGRGGEEIEHLAEAGISFQVVPGITAAS
                     GCASYAGIPLTHRDFAQSVRFVTGHLKDGSPDLNWPELAHADQTLVFYMGLLGLPHIC
                     RQLVAHGLDDQTPIALIQQGTTPNQQVWVATLATLPQLLERIRPQPPTLAIVGGVVSL
                     HRRLAWFQGGAPAAEQDQ"
     gene            2885..3772
                     /gene="dapA"
                     /locus_tag="PKI23_09645"
     CDS             2885..3772
                     /gene="dapA"
                     /locus_tag="PKI23_09645"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNN37239.1"
                     /translation="MIRGSIVALVTPMADDGRVSWSELAALVDFHLAAGTHAIVSMGT
                     TGESATLDFDEHIEVVRRTVERVQGRIPVIAGTGANSTREAIELTAAAKQAGADACLL
                     VTPYYNKPTQQGLYLHHRAIAEQVEIPQILYNVPGRTACDLLPETVARLAQLPNIIGI
                     KEATGSIERAKEILACCPAPFMLYCGDDAIAIELILLGAQGNISVTANVAPRQMAEAC
                     AAALAGDAARARQIDRALAPLHRDLFIESNPIPVKWALHEMGLISAGIRLPLTPLSPQ
                     HRPQLRQALQQAELLDRTD"
     gene            3833..4888
                     /gene="bamC"
                     /locus_tag="PKI23_09650"
     CDS             3833..4888
                     /gene="bamC"
                     /locus_tag="PKI23_09650"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNN37240.1"
                     /translation="MNTVAKGWVGLLLLGALTGCGTGLVRDRYYDYLDAEQGDTLKLP
                     PRLADAKAPEPLMPIPELASAPSNPERAPVPRPRPVAMQVSSAERVTLQQAGARNWLL
                     VLDPPAKVWPQMVRFFDESALPLKGSDPASGVLESGWLPSGGGSERFRVQVEEGVRPQ
                     SSEIKLLQQSATAAAPAVELPWPAQSSDTSREAALLERLQNHLRRSADADAGSFSLLA
                     QNLSSKKRSELIDPGKGEAPYLLLDVGYERAWSAVELALKELPRMQVEERNHAQGVIH
                     LHFAAGEEEKPNWFDTLIADLSGEKRAVADEGAIDQLRLIKVGGKTEIRLTNAQGTPL
                     PKERALPLLRQIQEHLF"
     gene            4927..5697
                     /locus_tag="PKI23_09655"
     CDS             4927..5697
                     /locus_tag="PKI23_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNN37241.1"
                     /translation="MRFASLGSGSRGNATLVAAGDTCLMVDCGFSLRETERRLARLGL
                     APSQISAILVTHEHGDHLRGVERLAATHGIAVHATPGTLRAAAFSSDVTQHPLDASRP
                     FAIGGIEIAPLSVPHDAAEPCQYTFSDGALKFGLLTDVGSCTAAMVEHFSGCHALMLE
                     CNHDLQLLSEGSYPPSLKRRVMGLHGHLSNGQAAGFLAAIDHRWLQHLVAAHLSEANN
                     RPELARSALARVMHCDEELVEIADQQAGLRWRQLFQRL"
     gene            5735..6448
                     /locus_tag="PKI23_09660"
     CDS             5735..6448
                     /locus_tag="PKI23_09660"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNN37242.1"
                     /translation="MQRGKELYSGKAKSVFETADAQRLIMLFRDDTSAFDGKKIEQLA
                     GKGKVNNLFNAFIMQKLAAAGIPTHFDRLLSDTESLVRRLKMIPVECVVRNIAAGSLC
                     RRLGVTEGIDLDPPTFEFFLKNDALGDPMVNEYHIRAFGWATPEQVERMKALSFAVND
                     ILKPLFLAGDMLLVDFKLEFGLFGEQMLLGDEFTADGCRLWDRQTRRKLDKDRFRQNL
                     GGVVESYHEVAQRLGVSID"
     gene            6488..6577
                     /locus_tag="PKI23_09665"
     tRNA            6488..6577
                     /locus_tag="PKI23_09665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6522..6524,aa:Ser,seq:cga)
     gene            6616..7444
                     /locus_tag="PKI23_09670"
                     /pseudo
     CDS             6616..7444
                     /locus_tag="PKI23_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            7485..>8054
                     /locus_tag="PKI23_09675"
     CDS             7485..>8054
                     /locus_tag="PKI23_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 family protein"
                     /protein_id="HNN37243.1"
                     /translation="MDHTTLSSLRDRHPAWRLLASPHAPLVASFLRRVFVAPNVRVMP
                     EADLAEALEDELFALREQLGADAYPKGASEYLNDWCAPDKGWLRKFYQPGTDEAQFDL
                     TPATEKAIAWLGSLTERPFVGTESRLLTLFALLEQIAAGTEADPIKRVEELRQRRDAL
                     DAEIARVLAGDAPLLDDTAVRERFLQFQQL"
BASE COUNT         1415 a         2600 c         2695 g         1344 t
ORIGIN      
        1 gctggcgctg gcggtcggca tcgcactggc acgccgtttc tgaacggcat cgacctctta
       61 tccatccctg tatccatgac ctgtgactga tcaagaaaat gttggaccca aagagatttc
      121 gcactgaact ggatgaactg gcggcccgtc tcgccagccg caacttcacg ctggatgtcg
      181 agcagatccg tcggctcgag gcgcgccgca aggcgttgca ggtcgatacc gaggcgctgc
      241 agaacgaccg caaccgccag gccaaggaga tcggccaggc caagcgtgac gggcgcgatg
      301 tcgcggcgct gctggccgcc ggcgaagatc tcggcgagcg gctgaccgcg ctgcagcagg
      361 agttgagtgc ggtgcaggag gcgctcgaac agattctgct cggcgtgccc aacctgcccc
      421 atgcctcggt accgatcggc cgtggcgagg cggacaacca ggagcagtcg cgctggggcg
      481 aaccgcgccg cttcgacttc acgccgctcg accatgtcgc actgggcgcc ggggcgggca
      541 tggatttcga ggccggcgcc aagctgaccg gcgcccgctt cgtggtgctc gaaggggggc
      601 tggcgcggct gcatcgggca ctgatccagt tcatgctcga tctgcacacc ggcgaacatg
      661 gctaccgcga gctctacgtg ccttacatcg tcaatgccga ttcgctgcgt ggcaccggtc
      721 agttgccgaa gttcgaggcc gatctgttca agctggtcgg cgaggagaac tactatctga
      781 ttcccaccgc cgaggtgccg gtcaccaatc tcgcccgcga ccgcatcgtc gaggccgagc
      841 agctgccgtt gaagtgggtc tgccacaccc cctgttttcg cagcgaggcg ggcagccatg
      901 gccgcgacac ccgcgggatg atccgccagc accagttcga gaaggtcgag ctggtgcaac
      961 tggtggcggc cgaggcctcc catgacgcac tcgaagttct gaccggccac gccgaagccg
     1021 tgctgcaacg gctcgaactg ccttaccgca agatggtgct ctgcaccggc gacatcggtt
     1081 tttcggctgc caagacctat gacctcgaag tttggctgcc aagccagaac tgctatcggg
     1141 agatctcctc ctgcagcaac ttcgaggatt tccagacccg gcggatgcag gcgcgacagc
     1201 gccatccggt cagccgcaag cccgagctgc tgcacagcct caatggctcg gggctggcag
     1261 tgggccgcac attgctggcg ctgatggaga acttccagca ggccgacggc agcatcgtgc
     1321 tgcccgatgc actgcgtccc tacctgggtg gcatggcgcg gctcgacctc gagcgtgcgg
     1381 gctgacgcag ccatggacta tctgccgatc tgtatcgact tgcgtggccg ccactgcctg
     1441 ctggtcggtg gcggcgaggt ggcgcggcgc aaggccgaga ccctgctgcg cgctggcgcg
     1501 acgctgcggc tggtggcgcc agcgatcgac cccggcctgg gtgaactggt gcggcagcgg
     1561 ggcggcgaac tgcggttgcg ggcgttcgag ccacgggatg tcgatgacgt ctggctggcg
     1621 gtggccgcca ccgacgatga ggcggtcaac cgtcaggtcc gcgacgcggc gcggcagcgg
     1681 ggggtgctgg tcaatgtggt cgatcagccg gcgctgtgcg acttcatctt tccatcgatc
     1741 atcgaccgcg atccgctgct catcgcggtg tcgagcggtg gccgggcgcc gctgctggcc
     1801 cggctgctgc gggcccggct cgaaaccacc attccggccg gctacgggcg gctggcggcc
     1861 tggatgggcc ggtttcgccc gcaggtgaag gcgcggattg ccggcggaac tgcccgccgc
     1921 cgcttctggg aacgggtgct ggagggccag atcggcgaga cctttctggc cggacgcgag
     1981 gccgaagccg agcggctgct gacggcgtcg ctgacggccg gcacggtcga tgaggtgggt
     2041 gaggtctatc tggtcggcgc cggacccggt gatcccgacc tgctcacctt ccgcgccctg
     2101 cggctgatgc agaaggccga tgtggtgctc tatgaccgcc tggtcgccgc gccgatcgtc
     2161 gatctggtgc gcaaggaggc cgagcgcatc catgtcggca aggagcgcga ccgccacacc
     2221 ctgccacagt cacgcatcaa ccagttgctg atcgatctgg cccgctccgg caagcgggtg
     2281 ctgcggctga aggggggcga ccccttcatc ttcggccgcg gcggcgagga gatcgaacat
     2341 ctggccgaag ccggcatctc ctttcaggtg gtgccgggca tcaccgccgc ctccggctgc
     2401 gccagctatg ccggcattcc gctgacccac cgcgactttg cccagtcggt gcgcttcgtg
     2461 accgggcatc tgaaggatgg ctctcccgac ctcaactggc cggagctggc ccatgccgac
     2521 cagacgctgg tcttctacat ggggctgctg gggctgcccc acatctgccg gcagctggtg
     2581 gcgcatggcc tcgacgatca gacgccgatc gcgctgatcc agcagggcac cacgccgaac
     2641 cagcaggtgt gggtggcaac gctggccacc ctgccgcaac tgctcgaacg gatcagaccg
     2701 cagccaccga cactcgccat cgtcggcggg gtggtgagcc tgcatcggcg gctggcctgg
     2761 ttccagggtg gcgcgccggc tgcggagcag gatcagtgag cggttgctgg tgttacactg
     2821 acgcgccgct ggcgacgccc ggcggatggc ggaagttgat cctggttcgt gaagaggagt
     2881 tgcaatgatc agaggcagta tcgttgccct ggtgaccccg atggccgacg acggccgcgt
     2941 gtcgtggagt gagctggcgg cgctggtcga tttccatctg gcggccggca cccatgccat
     3001 tgtctcgatg ggcaccaccg gcgagtcggc gacgctcgac ttcgatgaac acatcgaggt
     3061 ggtgcggcgc accgtcgagc gggtgcaggg ccgcatcccg gtgattgccg gcaccggcgc
     3121 caacagcacc cgcgaggcga tcgaactgac tgccgccgcc aaacaggccg gtgccgatgc
     3181 ctgcctgctg gtcacgccct actacaacaa gccgacccag caggggctct atctgcacca
     3241 ccgggccatt gccgagcagg tggagattcc gcagattctc tacaacgttc ccggccgcac
     3301 cgcctgcgat ctgctgcccg agaccgtggc ccggctggcg caactgccca acatcatcgg
     3361 catcaaggag gccaccggca gcatcgagcg ggccaaggag atcctggcct gctgtccggc
     3421 gcccttcatg ctctattgcg gcgatgacgc cattgccatc gagctgattc tgctgggcgc
     3481 ccagggcaac atctcggtca ccgccaacgt ggcgccgcgg cagatggccg aggcctgcgc
     3541 ggcggccctg gccggcgatg cagcgcgcgc gcggcagatc gaccgggcac tggccccgct
     3601 gcaccgtgat ctcttcatcg aatcgaaccc gattccggtc aagtgggcac tgcacgagat
     3661 ggggctgatt tccgccggca tccggctgcc gctgacaccg ctctcaccgc agcatcggcc
     3721 ccagctgcgc caggcgttgc agcaggccga actgctcgat cggactgact gagtgccatc
     3781 ggcacggcat gaagggtttt cgtgatacgc attgaactgg atcgataaaa acatgaacac
     3841 tgttgccaaa ggctgggtcg gcctgctgct gctcggtgcg ctgaccggat gtggaaccgg
     3901 tctggtgcgt gaccgctact acgactacct cgatgccgaa cagggcgaca cgctgaagct
     3961 gccgccacgg ctcgccgacg ccaaggcgcc cgagccgctg atgccgattc ccgagctggc
     4021 ctcggcgcca tcgaatccgg agcgcgcacc agtgccgcgt ccacggccgg tggcgatgca
     4081 ggtcagcagt gccgaacggg tcactcttca gcaggccggt gcgcgcaact ggctgctggt
     4141 gctcgatcca ccagccaagg tgtggccgca gatggtgcgc ttcttcgatg agtcggcgct
     4201 gccgctgaag ggctccgatc cagccagcgg cgtgctggag tccggttggc tgccgagcgg
     4261 tggcggcagc gagcgcttca gggtgcaggt cgaagagggg gtgcggccgc agagcagcga
     4321 gatcaaactg ctgcaacagt cggcgaccgc tgccgcgcct gcggtggagc tgccctggcc
     4381 ggcacagtcg agcgacacca gccgcgaagc cgcgctgctg gagcggctgc aaaaccatct
     4441 gcgtcgcagt gccgatgccg atgcgggctc attctcgctg ctggcgcaga acctcagcag
     4501 caaaaaacgc agtgaattga tcgatcccgg caagggtgaa gcgccctacc tgctgcttga
     4561 cgtgggctac gaacgggcct ggtcggcggt cgaactggcg ctcaaggagc tgccccggat
     4621 gcaggtcgag gagcgcaacc acgcccaggg cgtgatccat ctgcactttg ccgctggcga
     4681 agaggagaaa cccaactggt tcgacacgct gatcgctgac ctcagtggcg aaaagcgtgc
     4741 cgtggcggat gagggtgcca tcgaccagtt gcggctgatc aaggtgggcg gcaagaccga
     4801 gattcgactg accaacgcgc aggggacacc gctgccgaag gagcgcgccc tgccactgct
     4861 gcggcagatt caggagcatc tgttctgatc gctgcgcctg cgctctgcca ctgctgagcc
     4921 gcgcggatgc gtttcgcctc gcttggcagc ggcagccgtg gcaatgccac cctggtggcg
     4981 gccggcgaca cctgcctgat ggtcgattgc ggcttctcgc tgcgtgagac cgaacggcgg
     5041 ctggcccggc tggggctggc gccgtcgcag atcagcgcca tcctggtcac ccacgagcat
     5101 ggcgaccacc tgcgtggcgt cgagcggctg gcggcgaccc atggcatcgc ggtccatgcc
     5161 accccgggca ccctgcgggc cgccgccttt tcgtcggatg tgacccagca tccgctcgat
     5221 gccagtcggc ccttcgccat cggcggcatc gagatcgctc cactttcggt gccgcacgat
     5281 gccgccgagc cgtgccagta caccttcagc gacggagcgc tgaagtttgg cctgctgacc
     5341 gatgtcggct cctgcaccgc agcgatggtc gagcacttca gcggctgcca tgccctgatg
     5401 ctggagtgca accacgatct gcaactgctg agcgagggca gctatccacc ctcgctcaaa
     5461 cggcgggtga tggggttgca tggccatctc agcaatggtc aggccgccgg ttttctggct
     5521 gcaatcgacc accgctggct gcaacatctg gtggcggccc acctgagcga ggccaacaac
     5581 cggccggagc tggcgcgcag cgcgctggcg agggtgatgc attgcgacga ggagctggtc
     5641 gaaatcgccg accagcaggc gggattgagg tggcggcaac tgtttcagcg gctataatgc
     5701 cgcccttcga tcacccaacc gcccgagggg cgtgatgcag cgcggcaaag aactttactc
     5761 cggcaaggcc aaatcggtct ttgagaccgc cgacgcacag cggctgatca tgctgttccg
     5821 cgacgacacc agcgcgttcg acggcaagaa gatcgaacaa ctggccggca agggcaaggt
     5881 caacaacctg ttcaacgcct tcatcatgca gaagctggcc gccgccggca ttccgaccca
     5941 cttcgaccgg ctgctcagcg acaccgaatc gctggtcaga cggctgaaga tgattccggt
     6001 ggagtgcgtc gtgcgcaaca tcgccgccgg cagcctctgc cgtcgtcttg gcgtgaccga
     6061 gggcatcgac ctcgacccgc cgaccttcga attctttctg aagaacgacg cgctgggcga
     6121 cccgatggtc aatgaatacc acatccgcgc attcggctgg gccacgcccg aacaggtcga
     6181 gcggatgaag gcgctgtcgt tcgcggtgaa cgacatcctg aagccgctgt tcctcgccgg
     6241 cgacatgctg ctggtcgact tcaagctgga gttcggcctg tttggcgagc agatgctgct
     6301 gggcgatgag ttcaccgccg acggctgccg gctgtgggac cggcagacgc ggcgcaagct
     6361 cgacaaggac cgcttccgcc agaacctggg tggcgtggtc gagtcctacc acgaggtggc
     6421 acagcgtctc ggagtcagca tcgattgaag ctgcggcgtc tccccggcgc cgtgagcgaa
     6481 cgttttcgga gaggtgccag agcggccgaa cggggcggtc tcgaaaaccg ttgtgccttt
     6541 gcgggcaccg agggttcgaa tccctccctc tccgccaatt caagtccttc tattgatcca
     6601 gcatgattaa gattgattca tggcctctgc ccccaagggg ttgaatggac aaaagaagat
     6661 gcaagatttc agacgttgga gcgattgcac gagcgacgca aacaagttgt tcggctgcat
     6721 tgcaaaggga taggcgtgat gcggattgtc gagttggtgg gactgtcctg cccgaggatg
     6781 cgcggcgtgg acaactgcct ggcccgctgg ggcttcaacg ccgcaaagcc gaaggcctac
     6841 gagcaacgtc cggaagcgtg caggcctggc tggatgagca ataccccgag attgagcagc
     6901 gcgccaaggc cgaaggggcg gagattcact ggggcgatga aaccgccctg gtcaacaccg
     6961 atgtacgcgg acgcagtgac gtaccggcag ggaagacgcc cgtggcctgt gcagtgggta
     7021 gcacgcgcca gaagctgtcg atgattgcca ccgtcaccaa ccaaggcaag acgcgttgga
     7081 tgatcattga tcaggcgttc aactcggaca agctgattga gttcctggaa gccttgacca
     7141 aggagactgg gcaggaaggt attccggatt ctggacaacc tgcgggtcca gcaagccggt
     7201 caaggcctgg gctgctgagt ggaaagacaa gatcgaactg ttctgcctgt ccagctacag
     7261 ccccgaactg aatccggagg agtggctcaa cgctgacctc aaacaggcca tcggatcaaa
     7321 ggtgccggcg cgcaccaagg ccaagctgaa aaccgccgca accgagcaca tgacccaact
     7381 tgagcaaaac ccggatcgag tcatgtccta cttccaagac ccgcccgtca aatatgcggc
     7441 ttgaaacttc aaacttcatc tggctggatc aataacgccc agccatggac cacaccaccc
     7501 tcagttcgct gcgcgaccgc catcccgcct ggcggctgct ggcgtcaccg cacgcgccgc
     7561 tggtggcgag ctttctgcgc cgggtgttcg tggcgccgaa cgtgcgggtg atgcccgagg
     7621 ccgatcttgc cgaggcgctg gaggatgaac tgttcgcctt gcgcgagcag ctcggagcgg
     7681 atgcttaccc gaaaggtgct tcggagtacc tcaacgactg gtgcgcgccg gacaagggct
     7741 ggctgcgcaa gttctaccag ccgggcaccg acgaggcgca gttcgacctg acgcccgcca
     7801 ccgagaaggc catcgcctgg ctggggtcgc tgaccgagcg ccccttcgtc ggcaccgagt
     7861 cgcgtctgct gacgctgttc gcgctgctgg agcagatcgc cgccggcacc gaggccgacc
     7921 ccatcaagcg ggtcgaggag ttgcggcaaa gacgcgacgc gctcgacgcc gagatcgccc
     7981 gcgtgctggc cggcgacgct ccgctgctgg acgacacggc ggtccgcgag cgcttcctgc
     8041 agttccagca actg
//
LOCUS       DAORCQ010000101         8034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6285_length_8034_cov_5.790826, whole genome shotgun sequence.
ACCESSION   DAORCQ010000101 DAORCQ010000000
VERSION     DAORCQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6285_length_8034_cov_5.790826"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKI23_09680"
     CDS             complement(<1..354)
                     /locus_tag="PKI23_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HNN37244.1"
                     /translation="MSGFYASADVGNFDMYDDILTFDEVDEEFETLDDTSALDEGLSA
                     VLGRASRRVTHGVTTVEGSNASINVTLQKNLDATQIYLSEIGFTPLLSAREEVFFARR
                     ALRGELKARNRMIESN"
     gene            complement(407..1195)
                     /locus_tag="PKI23_09685"
     CDS             complement(407..1195)
                     /locus_tag="PKI23_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNN37245.1"
                     /translation="MHIAARQHPFDPRNSRWLALLGITLLLCACASPLPLSDHDDATA
                     ENKSSKTEPRRTPPREYVVVKGDTLVAIAFRHGMDYRQLAALNGIKEPYTIYPGQRLR
                     LSRDTSASSAGRSTPVNAKGSPVRTPAPAETSSVTSTLPWRWPVAGKVLRGYDSAGGT
                     NKGIDIAAPEGTQVLAAAGGTVVYAGNGLRAYGNLLIIKHNDEFLSAYGYNRRLLVAE
                     AEEVKVGQPIAETGSDGQTTGLLHFEIRRQGKPIDPLLYLPRQP"
     gene            complement(1241..1909)
                     /locus_tag="PKI23_09690"
     CDS             complement(1241..1909)
                     /locus_tag="PKI23_09690"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNN37246.1"
                     /translation="MRPGTLGIGMTSVRTRERLIDRLRQQGISNRQVLEAMREVPRHL
                     FIDEAMAHHAYEDKALPIGFNQTISQPYTVARMLEALLAHGTPEQVLEVGTGSGYQTA
                     LLAELVPKVFTIERIRPLLLRARQRLRQLGYRNVLSDHVDGHQGWPGGGEFGGIIVSA
                     APATLPEALLAQLAAGGCMVTPVGRLQNQLLKRITRSSEGLLREETLERVLFVPLIPG
                     ALSG"
     gene            complement(1909..2667)
                     /gene="surE"
                     /locus_tag="PKI23_09695"
     CDS             complement(1909..2667)
                     /gene="surE"
                     /locus_tag="PKI23_09695"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007250172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNN37247.1"
                     /translation="MNLLISNDDGVFAPGIAALTRELARLGPLTVVAPDRDRSGASNS
                     LTLDRPLYPRTHDNGFISIAGTPTDCVHLGIHSLSAQPPDRVVSGINAGANLGDDVLY
                     SGTVAAAMEGRALHATPIAVSLVRQGENELHHYQTAAIIVRRLIEQADRLHLEPRTVL
                     NVNVPDLPLDQLQGIKLTRLGHRGVSPGPTPFQDPRGRQGYWIGQMGLEQDAGPGTDF
                     DAVRHGYVSVTPLQIDMTRYEAFDTLTRWLESLR"
     gene            complement(2664..3203)
                     /gene="ispF"
                     /locus_tag="PKI23_09700"
     CDS             complement(2664..3203)
                     /gene="ispF"
                     /locus_tag="PKI23_09700"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNN37248.1"
                     /translation="MRIGHGFDVHRLVDGSDHSAGTRPLVLGGVAIPCAFRIVAHSDG
                     DVLLHALMDALLGAAALGDIGHWFPDSDPAYAGVDSRQLLQHCLAQISQQGWQLANAD
                     LTLVAQQPRIAVHVPQMRATIAESLGIQTCQINVKATTTERLGAIGRGEGIAAHAVAL
                     LQRATPGNQATPSQGITAS"
     gene            complement(3203..3949)
                     /gene="ispD"
                     /locus_tag="PKI23_09705"
     CDS             complement(3203..3949)
                     /gene="ispD"
                     /locus_tag="PKI23_09705"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNN37249.1"
                     /translation="MSASPPTRSTTPLWAVVPAAGLGSRLGAELPKQYLPLLGRAMIE
                     HTLERLLQLTEIQAIVVVLHPHDRWWPTLGLHDHPRITTVTGGAERCHSVLAGLRRLR
                     EQAAGHQRVLVHDAARPCVRPADIRTLIEQTTHHADGGLLGVAVRDTMKRLDETHQVQ
                     QTVSRTRLWHAQTPQLFTLEPLITALEQATTRQQLVTDEAEAMELIGHRPLLVAGSDD
                     NIKVTRSEDLPLAEFHLQRQVAQFAREASA"
     gene            complement(3949..4284)
                     /locus_tag="PKI23_09710"
     CDS             complement(3949..4284)
                     /locus_tag="PKI23_09710"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNN37250.1"
                     /translation="MNRLRREHRLWLALLLLFLLAALQLSLWSGVGSRLELQRLQQGI
                     ERQQQENALLQARNEQIGLEVTSLKSGLEQIEVKAREELGLVRPNETLFLFVNEQTHA
                     GQRPGPQPR"
     gene            complement(4303..5592)
                     /gene="eno"
                     /locus_tag="PKI23_09715"
     CDS             complement(4303..5592)
                     /gene="eno"
                     /locus_tag="PKI23_09715"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNN37251.1"
                     /translation="MTATIRQIRAREVLDSRGNPTIEADVLLHSGVVGTACAPSGAST
                     GSREALELRDGDPARYGGKGVLQAVGHVNGPIGALLKGRDATDQAALDQAMIEADGTE
                     NKSKFGANAILAVSLAAAKAAAQSLGKPLYQHIAELNGTTQLTLPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAPSFAEALRCGAEIFHSLKKVLHGRGLATSVGDEGGFAPNLPS
                     NEAALEAILEAIQKSGYQLGKEVTLALDCAASEFYHDGHYRLTGEGKSYDAGGFADYL
                     ATLAGKYPILSIEDGMHESDWSGWKQLTQLIGERVQLVGDDLFVTNTRILQRGIEQGI
                     ANSILIKFNQIGSLTETLAAIRMAKAAGYTTVISHRSGETEDTTIADLAVATAAGQIK
                     TGSLCRSDRVAKYNRLLRIEEELQGRAPYRGRAEFRG"
     gene            complement(5644..6477)
                     /gene="kdsA"
                     /locus_tag="PKI23_09720"
     CDS             complement(5644..6477)
                     /gene="kdsA"
                     /locus_tag="PKI23_09720"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNN37252.1"
                     /translation="MQLCGFEVGLDRPFFLIAGPCVIESEQLALDTAGELAELCRALD
                     IPFIYKSSFDKANRSSHQSYRGPGIDAGLRILEQVKTQIGVPVLTDVHEETPIDEVAS
                     VVSVLQTPAFLCRQTNFIQRVAAAGLPVNIKKGQFLAPWDMAHVVAKARATGNPQIMV
                     CERGVSFGYNNLVCDLRSLAVMRETGAPVVFDATHSVQLPGGQGSASGGQREMVPVLA
                     RGAVAAGVAGLFMETHPDPDRAKSDGPNSWPLARMGELLHTLRDIDHLVKQQGLIETT
                     L"
     gene            complement(6491..>8034)
                     /locus_tag="PKI23_09725"
     CDS             complement(6491..>8034)
                     /locus_tag="PKI23_09725"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNN37253.1"
                     /translation="KLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFLHAR
                     MSRLNNFTTGRVYETVLRKERRGDYLGGTVQVIPHITDEIKRRIIEGVGNVDVALVEV
                     GGTVGDIESQPFLEAIRQLKLELGAQHCLFMHLVLIPYIASAGETKTKPTQHSVKEMR
                     SIGLQPDILICRSDHEIPEDARRKIALFTNVEQRAVIPLLDTDTIYRIPLLLQQSGLD
                     EIVTDMLRIDCRQADLTPWRQVVEAQLNPRQQITIAMVGKYINLLDAYKSLNEALIHA
                     GIHSRTRVTIRYIDSEELESSDIDKLIGVDAILVPGGFGVRGIEGKLKAVRHAREQGI
                     PYLGICLGMQLAVIELARNRLGLPQANSTEFDPITPDPVIGLITEWADRSGSIETREE
                     STDLGGTMRLGAQECRLVKQSRIRELYGRDIITERHRHRYEVNNNYLERLQQQGMKIS
                     GWSMDDALVEAIELEDHPWFVACQFHPEFTSSPLDGHPLFSGFVRAALDHRQRSGRPL
                     AIHSD"
BASE COUNT         1311 a         2441 c         2759 g         1523 t
ORIGIN      
        1 gttgctctcg atcatgcggt tgcgggcttt cagttcaccg cgcagtgcgc ggcgggcgaa
       61 gaagacctcc tcgcgggccg acagcagtgg cgtgaagccg atttcgctga ggtagatctg
      121 ggtggcgtcg agattcttct gcagcgtgac attgatcgag gcgttgctgc cttcgacggt
      181 ggtcactcca tgggtcacac gccggctcgc tctgcccagt acggcggaca acccctcatc
      241 cagcgccgag gtgtcatcga gggtttcgaa ctcctcgtcc acctcatcga aggtgagaat
      301 gtcatcgtac atgtcgaagt tgcccacatc tgctgacgca tagaacccgc tcattgttct
      361 gctcccaaac atcactgtca atcgaggtca ttgggactgg aatccgctag ggctgccgag
      421 gcaggtagag cagcggatcg atgggtttgc cttgacgacg aatctcgaag tgcagcaatc
      481 cagttgtttg cccatcactt cctgtttcgg caatgggctg cccaacttta acctcttctg
      541 cttcggcgac cagcaatcga cggttgtagc cgtaggcgct caggaactcg tcgttgtgtt
      601 tgatgatcag caggttgccg taggcacgca gtccattgcc ggcgtagacc acggttccgc
      661 cggcagccgc cagtacctgg gtgccttcgg gtgcggcgat gtcgatgccc ttgttggtgc
      721 cgccggcgct gtcataaccg cgcagcacct tgccggccac cggccagcgc cagggcagcg
      781 tgctggtgac ggaggaagtt tctgccggag ctggagttct gaccggtgag cctttggcat
      841 tgacgggggt tgagcgaccg gcgctgctgg cggaggtgtc gcggctgagc cgcaggcgct
      901 gaccgggata gatggtgtag ggctccttga tgccattgag ggcggccagt tgacggtaat
      961 ccatgccatg acgaaaggcg atggcgacca gtgtgtcgcc cttgactacg acatactctc
     1021 gtggtggggt tcggcgaggt tcggtcttgc tgctcttatt ctctgcggtg gcgtcgtcgt
     1081 ggtcggacag cggcagcggc gaggcacagg cgcagagcaa cagggtgata cccagaaggg
     1141 cgagccagcg gctgttccgt gggtcgaagg gatgctgccg ggccgcgatg tgcatggagg
     1201 aggcagtccg agcgggtggt ccggccatgg cgggcagggc tcagccggag agggcgccag
     1261 ggatcagcgg cacgaacagc acccgctcca gcgtctcttc gcgcagcaga ccctcactgg
     1321 agcgggtgat gcgtttgagc aattgattct gcaggcgacc gaccggcgtc accatgcagc
     1381 cgcctgctgc cagttgggcc agcagtgcct ccgggagcgt ggcgggtgcc gccgagacga
     1441 tgatgccgcc aaattcgccg ccgccgggcc agccctgatg gccatcgaca tggtcggaga
     1501 ggacattccg gtaacccagt tgacgcagtc tctgtcgtgc ccgcagcagc agcgggcgaa
     1561 tgcgttcgat ggtgaagacc ttgggaacca gttcggcaag cagcgcggtc tggtaaccgg
     1621 agccggtgcc gacctccagc acctgctcgg gcgtgccatg ggccagcagg gcctcgagca
     1681 tccgcgcgac ggtgtagggc tgcgagatgg tctggttgaa gccgatcggc aatgccttgt
     1741 cttcataggc gtggtgtgcc atcgcctcgt cgatgaagag gtggcgcggc acttcgcgca
     1801 ttgcctccag cacctgtctg ttgctgatgc cctgctggcg caaccggtcg atcagccgct
     1861 cacgggtgcg gaccgaggtc atgccgatgc cgagcgtgcc ggggcgcatc agcgcagact
     1921 ctccagccag cgggtcaggg tgtcgaaggc ttcatagcgg gtcatgtcga tctgcagtgg
     1981 cgtgaccgag acatagccat ggcgcaccgc gtcgaagtcg gtgccggggc cggcatcctg
     2041 ttccaggccc atctgtccga tccagtagcc ctgccgaccg cgcggatcct gaaacggggt
     2101 ggggccgggc gagacaccac ggtggccgag ccgggtcagc ttgatcccct gcaactgatc
     2161 gagcggtaga tcgggcacat tgacattgag gacggtgcgg ggctccagat ggagccgatc
     2221 ggcctgctcg atcaggcgtc gcacaatgat ggcggcggtc tgatagtggt gcagttcgtt
     2281 ttctccctgt cgaaccagcg aaacggcaat cggcgtggca tgcagcgcgc ggccttccat
     2341 cgcggccgcc actgtgccgg agtagagcac atcatcgccg aggttggcac cggcattgat
     2401 gccggagacg acgcggtcgg gcggttgcgc actgagcgaa tggatgccga ggtggacgca
     2461 gtcggtcggg gtgccggcaa tgctgatgaa gccattgtcg tgggtgcgtg gatagagtgg
     2521 acggtcgagg gtcagagagt tgctggcacc gctgcggtca cggtcgggtg ccaccactgt
     2581 caaggggccg agtcgggcca gctcccgggt cagggcggcg atgcccgggg cgaaaacgcc
     2641 gtcgtcgttg gagatcaaca gattcatgat gcagtgattc cttggctggg tgtcgcttga
     2701 ttgcccgggg tggcacgctg gagcagagcc accgcgtggg cggcgatgcc ctcgccccga
     2761 ccgatggcgc cgagccgttc ggtggtggtg gccttgacgt tgatctggca cgtctggatg
     2821 ccaagcgatt cagcgatggt ggcccgcatc tgtggtacat gaacggcgat gcgcggctgc
     2881 tgggccacca gcgtcagatc ggcattggcc agttgccagc cctgctggct gatctgcgcc
     2941 agacagtgtt gcagcaactg acgactgtcg acacctgcgt aggccggatc gctgtcgggg
     3001 aaccagtggc cgatgtcgcc gagggcggcc gcgcccaaca gtgcatccat caatgcatgg
     3061 agcagcacgt cgccatcgga gtgggcgacg atgcgaaagg cgcaggggat ggccacgccg
     3121 cccagcacca gcggtcgggt gccggccgag tggtcgctgc catcgaccag acgatggacg
     3181 tcaaagccgt gaccgatgcg catcatgcgc tggcttcccg agcgaattgc gccacctggc
     3241 gctgcagatg gaactcggcc agcggcagat cctcgctgcg ggtcaccttg atgttgtcgt
     3301 cactgccggc caccagcagc gggcgatggc cgatcagttc catcgcctcg gcctcgtcgg
     3361 tgaccagttg ctgacgggtg gtggcctgtt ccagcgctgt gatcagcggc tccagcgtga
     3421 acagttgcgg ggtctgggca tgccagagcc gggttcgcga gacagtctgt tgcacctgat
     3481 gggtctcgtc cagccgcttc atggtgtcgc gcaccgccac gccgagcagc ccgccgtcgg
     3541 catggtgtgt ggtctgttcg atcagtgtgc ggatgtcggc cgggcgcaca caggggcggg
     3601 cggcatcgtg caccagcacc cgttgatggc cagccgcctg ttcgcgcagc cggcgcaggc
     3661 cggccagcac cgagtggcag cgttcggcac cgcccgtgac agtggtgatg cgtgggtggt
     3721 cgtgcaggcc aagagtgggc caccagcggt cgtgcggatg cagcacgacg acgatggctt
     3781 gaatttcagt cagttgcagc agccgttcga gcgtgtgctc gatcatcgcc ctgccgagca
     3841 gcggcaggta ttgcttcggc agctcggcgc ccagacggct gccaagaccc gcggcgggca
     3901 ccaccgccca cagtggggtc gttgatctgg tcggtggcga ggcactcatc agcggggctg
     3961 tggcccgggc ctctgaccag cgtgggtctg ttcattgacg aacaggaaca gcgtctcgtt
     4021 cgggcgaacc aggcccagct cttcgcgggc cttcacctcg atctgctcca gcccgctctt
     4081 cagcgaggtg acttcgagtc cgatctgctc gttgcgggcc tgcaacaggg cattctcctg
     4141 ctgctggcgc tcaatgccct gttgcagccg ctgcagttcc aaccggctgc caaccccgct
     4201 ccacagactg agctgcaggg ccgccagcag aaacagcagc agcagagcga gccagagacg
     4261 atgctcacgc ctgagtctgt tcacgcatca cgctcgccgc ggtcacccgc gaaactcagc
     4321 ccgaccccgg tatggcgcac ggccctgcaa ctcctcttcg attctgagca gacggttgta
     4381 tttggcaacc cggtccgagc ggcagagcga cccggtcttg atctgtccgg cagcggtggc
     4441 gacggccaga tcggcgatgg tggtgtcttc ggtctcgccg gagcggtggg agatcaccgt
     4501 ggtgtaaccc gcggccttgg ccatgcggat cgcggccagg gtctcggtca gactgcctat
     4561 ctgattgaac ttgatcagga ttgagtttgc aatcccctgt tcgatgccgc gttgcagaat
     4621 ccgggtgttg gtgacgaaca ggtcatcgcc gaccagctgc acgcgctcgc cgatcagctg
     4681 ggtgagctgc ttccagccac tccagtcgct ctcgtgcatg ccatcctcga tcgacaggat
     4741 cggatacttg ccggccagtg tggccaggta gtcggcaaaa ccgccggcgt catagctctt
     4801 gccttcgccg gtgagccgat agtggccatc gtggtagaac tccgaggcgg cgcagtcgag
     4861 cgccagggtc acctccttgc cgagttgata gccgcttttc tggatcgctt cgagaatcgc
     4921 ctcgagggcg gcttcgttgc tcggcaggtt gggggcaaag ccgccctcat cgccgaccga
     4981 ggtggcgagt ccacgcccat gcagcacctt cttcaggctg tgaaagatct cggcgccgca
     5041 gcgcagcgct tcggcaaagc tgggcgcgcc gaccggctgg atcatgaact cctggatgtc
     5101 gacgttgttg tcggcatgtt cgccgccgtt gatgatgttc atcatcggca ccggcagcgt
     5161 cagttgcgtg gtgccgttga gttcggcgat gtgctggtag agcggcttgc cgagtgactg
     5221 ggcggccgcc ttggcggcgg ccagcgagac ggcgaggatg gcattggcgc cgaatttcga
     5281 cttgttttcg gtgccgtcgg cttcgatcat cgcttggtcg agtgcggcct gatcggtggc
     5341 atcacgcccc ttcagcagcg ccccgatcgg gccgttgaca tggcccaccg cctgcagaac
     5401 gcccttgccg ccgtagcggg ccgggtcgcc atcgcgcagt tccagtgcct cccgtgagcc
     5461 ggtcgaagcg ccagacgggg cgcaggcagt gccgacgacg ccggagtgca gcagcacgtc
     5521 ggcctcgatg gtcggattgc cgcgggagtc gagtacctcg cgggcgcgga tctgtctgat
     5581 ggttgccgtc attgcctcat ttctccatgt cagcgcggtg ctgccgatcg gcagcgttgg
     5641 gattcacagg gtggtttcga tcagtccctg ctgtttgacc aggtggtcga tgtcacgcag
     5701 ggtgtgcagc agctcgccca tccgggccag cggccaggag ttggggccat cgctcttggc
     5761 gcggtcggga tcggggtggg tctccatgaa gagtccggcg acaccggcgg cgactgcgcc
     5821 gcgggccagc accggcacca tttcgcgctg cccaccggag gcgctgccct ggccgccggg
     5881 caactgcacc gagtgggtgg catcgaacac caccggtgcg ccggtctcgc gcatcaccgc
     5941 cagcgatcgc aggtcacaga ccaggttgtt gtagccgaag ctgacgccgc gctcgcagac
     6001 catgatctgc gggttgccgg tggcgcgcgc cttggcgacg acatgggcca tgtcccatgg
     6061 ggcgagaaac tgccccttct tgatgttgac cggcaggccg gcggccgcca cccgctggat
     6121 gaagttggtc tggcggcaca gaaaggcggg cgtctgcagc accgagacca ccgaggccac
     6181 ctcgtcgatc ggcgtctctt catggacatc ggtcagcacc ggtacgccga tctgcgtttt
     6241 gacctgctcg aggatgcgca ggccggcatc gatgcccggt ccgcggtagc tctgatgcga
     6301 cgaacggttg gccttgtcga acgacgattt gtagatgaaa ggaatgtcga gagcgcggca
     6361 gagttcggcc agctcgccgg cggtgtcgag cgccaactgc tccgattcga tgacacaggg
     6421 gccggcaatc agaaagaacg gccggtcgag ccccacttca aagccacaga gttgcatggt
     6481 cgcgcctcat tcagtcggag tggatggcaa gtggtcggcc actgcgctgg cggtggtcga
     6541 gcgccgcacg cacgaagccg ctgaacagcg gatggccgtc gagcggtgag gaggtgaact
     6601 cggggtggaa ctggcaggcg acgaaccagg ggtggtcctc cagttcgatc gcttccacca
     6661 gtgcatcgtc catcgaccag ccggagatct tcatgccctg ctgctgcaac cgttcgaggt
     6721 agttgttgtt gacctcgtaa cggtggcggt ggcgctcggt gatgatgtcg cggccgtaga
     6781 gttcacgaat cctgctctgc ttgaccagtc gacactcctg ggctcccagc cgcatggtgc
     6841 cgccgagatc cgtgctctct tcgcgggtct cgatgctgcc gctgcggtcg gcccactcgg
     6901 tgatcagccc gatcaccggg tcgggagtga tcggatcgaa ctcggtgctg ttggcctgcg
     6961 gcagccccag ccggttgcgc gccagctcga tgaccgccag ttgcatgccg agacagatgc
     7021 ccagataggg gatgccctgt tcgcgggcat ggcgcaccgc cttcagcttg ccctcgatgc
     7081 cgcgcacacc gaaaccgccg gggaccagaa tcgcatcgac gccgatcagt ttgtcgatgt
     7141 cgctgctctc cagctcctcg gagtcgatgt agcggatcgt cacccgggtg cggctgtgga
     7201 tgccggcatg gatcagtgct tcgttgagcg acttgtaggc atcgagcaga ttgatgtact
     7261 tgccgaccat cgcaatggtg atctgctgcc gggggttgag ctgcgcctcg accacctgcc
     7321 gccagggggt caggtcggcc tgccggcagt cgatgcgcag catgtcggtg acgatctcat
     7381 cgagcccgct ctgctgcagc agcagcggaa tgcggtagat ggtgtcggtg tcgagcagcg
     7441 ggatcaccgc gcgctgctcg acattggtga agagcgcgat cttgcgccgg gcatcctcgg
     7501 gaatttcatg gtcggagcgg cagatgagga tgtcgggctg caagccgatc gagcgcatct
     7561 ccttcaccga gtgctgggtc ggtttggtct tggtctcgcc ggcgctggcg atgtagggaa
     7621 tcagcaccag atgcatgaac aggcagtgct gggcacccag ttccagcttg agctgacgaa
     7681 tcgcctccag aaagggctgt gactcgatgt cgccgaccgt gccgcccact tcgaccagcg
     7741 cgacgtcgac attgccgacc ccctcgatga tgcgacgctt gatctcgtcg gtgatgtgcg
     7801 gaatcacctg caccgtgccg cccaggtagt cgccgcggcg ctccttgcgc agcacggtct
     7861 cgtagacccg accggtggtg aagttgttca gccggctcat gcgcgcatgc agaaaacgct
     7921 catagtggcc gaggtcgagg tcggtctccg caccatcctc ggtgacgaag acctcgccat
     7981 gctggaacgg gctcatggtg cccggatcga cgttgatgta gggatcgagc ttga
//
LOCUS       DAORCQ010000102         7977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6359_length_7977_cov_5.350669, whole genome shotgun sequence.
ACCESSION   DAORCQ010000102 DAORCQ010000000
VERSION     DAORCQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6359_length_7977_cov_5.350669"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..2809)
                     /gene="prsT"
                     /locus_tag="PKI23_09730"
     CDS             complement(32..2809)
                     /gene="prsT"
                     /locus_tag="PKI23_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNN37254.1"
                     /translation="MPNNRRHCARLALVVLLTGVAGCGREPTAAEYLAKAQHYQQQGQ
                     YKEASIEARNAVKLAPDDPAGRLLLGNLYLQMGIYDAAEKELRRAHALGTAAELVNVG
                     FGEALLALGNWQEALKLAEQLDPARASEPLRLQLLRADAELAGERLDAAEQRYRDILK
                     QDARAARAHLGLARIAAIKGDAAAVNREIDSAAAIDSDDAELWSLKGEWQRLNGQTEA
                     AEQSYSEALRRRPNLINDRYHRALARIVLNNPSGADEDIQLLHKQLGDHPLVAYLQGM
                     NQFRQRHFDEAQSALEKAVRGNSRHFTSHYYLGLTHLLRNQPAQAEQYLKQALEIVPR
                     SAAARLALALSQYRSQQHDSAAKNLTELLKQQPDDVAALNLLGEVELKRGNSAAGLRH
                     LEAALKQGANSPAAQARLGLANLQAGRTDEGIEDLEALLKSDPRLAQASAMAALSKIQ
                     SGQLDEAAKVIALLKRNDDKGPLPDHLEGLLLIARNQPDAARAAFGRALQKEPGNPAA
                     GQNLALLAIAARHYDEAAGHYQAVLAKHPDDLQTLMGLAQLHALQGQSEPMVQRLEQA
                     HASHPRQAEPVIALARWQQQQGEVGKARTLLESAFADSDEGQLLLRALVELEIRAKES
                     ERALAALERLDPDSLANLRFSLDSYQRLNRPDRGLALLRSVTERHPAAPAPRLLLAQW
                     LLASNEIEQARQAFEPLRQRHPEQIEVLLFEGELARRQGKPELAVAAYQRAYKKQPSA
                     SLARALAQAQLQQGQPRAALATLEGWNRQAPNDLEALYLRAKLHGGLDEAPAARKLLE
                     QMVAIDPNSIVALNDLAWELKTSDPQRAGRYIDQALTRSPDAAALLDTQAMVRLAQGN
                     PVEAKAALDRALAIEPANRDYLYHQALLLHHAGKNEEAATQLRALLQQQQPFAERNEA
                     EALLRRLEAGR"
     gene            2999..3517
                     /gene="fabA"
                     /locus_tag="PKI23_09735"
     CDS             2999..3517
                     /gene="fabA"
                     /locus_tag="PKI23_09735"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HNN37255.1"
                     /translation="MSRPHAFDRDALLQCGHGELFGPGNAQLPVPNMLMLDRITLITD
                     DSGAHGKGEITAELDIRPDLWFFSCHFPGDPVMPGCLGLDAMWQLVGFFLAWRGNPGR
                     GRALGCGEVKFFGQVLPQAQKVTYHIQLKRVIERKLVMGIADGTMAVDGTTIYSARDL
                     RVGLFQSTERMG"
     gene            3640..4857
                     /gene="fabB"
                     /locus_tag="PKI23_09740"
     CDS             3640..4857
                     /gene="fabB"
                     /locus_tag="PKI23_09740"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HNN37256.1"
                     /translation="MRRVVVTGLGIVSCLGNDRQSVTDSLREGRSGIARIDDYVQMGL
                     KSHIGGTPKVDFEAAIDRKLLRFMGDAAAYAYLAMEQAISDARLLPQQISDPRVGLIM
                     GSGGASTANQVEAVDLMRDKGLRRVGPYRVTRTMGSTVSACLATPFQIKGVNYSISSA
                     CATSAHCIGNAMELIQLGKQDMVFAGGAEEIYWAMTMMFDAMGALSTQYNDQPQRASR
                     PYDRDRDGFVIAGGAGVLVLEELEHAQRRGAPILAELVGYGATSDGHDMVAPSGEGAA
                     RCMHQALAGARRPVDYINAHGTSTPVGDLCELGAIREVFGDEAPPIGSTKSLSGHSLG
                     AAGVQEAIYSLLMMEQNFLAASANIDNLDPGAEGLPILRTRRDGVELNSVLSNSFGFG
                     GTNACLLFERFEG"
     gene            complement(4897..6129)
                     /locus_tag="PKI23_09745"
     CDS             complement(4897..6129)
                     /locus_tag="PKI23_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNN37257.1"
                     /translation="MGGTPLTPVDVAIEQLLAAADGAPAVQPGATLDALGRVLARDLH
                     APFDQPAVDNSAVDGYAVRSREVEAGRPLPISQRIAAGDVAAELVPGTVARIFTGAPL
                     PAGADAVVMQEDADPEADQVRFTVTPRPGANVRRRGHEIAAGSLLLPAGTRLRPQELG
                     LIASAGVAQIPLCRPLRVALLSTGRELLEPGQGGAEQLASGARLFNSNRYLLTGLLRG
                     LGCQIDDFGIVDDTLAATRAALQQAAAVADLLITTGGASVGEEDHLISAIQQLGRLKL
                     WQLAIKPGKPFAFGEIGTTPIMALPGNPAAVLVTFCILCRPYLLRRQGVREVAPLRVG
                     VPAGFSVSQPGKRREYLRARLLRDATGHDRLEPHPNQGSGVLRSAAWADGLAVVEPGQ
                     RVTAGDLIDFHPFAALLG"
     gene            complement(6132..6662)
                     /gene="moaB"
                     /locus_tag="PKI23_09750"
     CDS             complement(6132..6662)
                     /gene="moaB"
                     /locus_tag="PKI23_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNN37258.1"
                     /translation="MSHAATEFVPLDLAVLTVSDSRDEASDSSGRFLAEAARDAGHRV
                     RDKRIVPDDRYQIRAAVSVWIADPAIQVVLVTGGTGFSQRDGTPEALAPLFDQHIDGF
                     GELFRHFSLEEIGSSTIQSRALAGLANRTLIFVLPGSTGACQTGWNRIIREQIDSRQR
                     PCSFVGHLLGTTSGGQ"
     gene            6808..7458
                     /locus_tag="PKI23_09755"
     CDS             6808..7458
                     /locus_tag="PKI23_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNN37259.1"
                     /translation="MKKSMAMATLCLLAVGTGSAYAGEGPWLVRLRALDMNVDNENNI
                     TPVTTAYGEVDVEDKWFPEIDISYFFTPNIAAELVLTYPQKHDVKLGNFDIGSLKHLP
                     PTLLLQYHFCPEGTVRPYVGAGINYTHFSDVDLNAAPAVGTDAPLRLDTSSWGLAAQV
                     GVDFKVAPNWFVNLDAKYVQIDANDIRVKSGVLAGTYVTDLTVNPWLLSVGAGYRF"
     gene            7635..>7977
                     /locus_tag="PKI23_09760"
     CDS             7635..>7977
                     /locus_tag="PKI23_09760"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNN37260.1"
                     /translation="MMTQPAGLTAQIAAQAAHRTFEHLPASMVALAKQCLLDWLGVTL
                     AGSREPLVETLLAQSCSEGGRPQATAVGHAPRVNRLQAALLNGTASHALDYDDVNMAI
                     IGHPTAPVLPAA"
BASE COUNT         1174 a         2619 c         2801 g         1383 t
ORIGIN      
        1 tttggcggct gggtcgagga agcccatgtc actaccgccc cgcctccagc cgccgcagca
       61 gtgcctcggc ctcattgcgt tcggcaaagg gctgctgttg ttgcagcagc gcgcgcaact
      121 gggtcgcggc ctcttcattt ttgccggcat ggtgcagcag caatgcctgg tggtagaggt
      181 agtcgcggtt ggctggttcg attgccagcg cgcggtcgag ggcggccttg gcctcgaccg
      241 ggttgccctg cgccagcctg accatcgcct gggtgtcgag cagcgcggcg gcgtcgggag
      301 agcgggtcag ggcctggtcg atgtagcggc cggcgcgctg cggatcgctc gtcttcagct
      361 cccaggccag gtcattgagg gcgacgatgc tgttggggtc gatggccacc atctgctcca
      421 gcagtttgcg cgccgccggc gcctcatcaa gcccgccatg cagcttggcg cgcaggtaga
      481 gcgcctcgag gtcgttgggt gcctggcggt tccagccctc caatgtggcg agcgcggcgc
      541 gcggctgccc ctgttgcagt tgcgcctggg ccagcgcgcg ggccagtgag gcgctgggct
      601 gctttttgta ggcgcgctgg taggcggcca ccgccagctc gggcttgccc tggcgccgcg
      661 ccagttcacc ctcgaacagc agcacctcga tctgctcggg gtggcgctgg cgcagcggtt
      721 cgaacgcctg acgcgcctgt tcgatttcat tgctggccag cagccactgc gccagcagca
      781 gccgcggtgc tggcgcagcg gggtggcgct cggtgacgct gcgcagcagc gccaggccgc
      841 ggtcggggcg gttgagccgc tggtagctgt cgaggctgaa gcgcaggttg gccagcgagt
      901 cggggtcgag ccgttcgagt gcggccagcg cccgctcgct ctccttggcg cgaatctcca
      961 gttcgaccag tgcccgcagc agcagttggc cctcgtcact gtcggcaaag gcgctctcga
     1021 gcagtgtgcg cgccttgccg acctcgccct gctgctgttg ccagcgcgcc agcgcgatga
     1081 ccggttcggc ctggcgcgga tggctggcgt gggcctgctc cagccgctgc accatcggct
     1141 cgctctgccc ctgcagcgca tggagctggg ccagccccat cagggtttgc aggtcgtcgg
     1201 gatgcttggc gagcacggcc tggtagtggc cggcggcttc gtcgtagtgg cgggcggcaa
     1261 tcgccagcag cgccaggttc tggccggcgg ccgggttgcc gggctctttc tgcagcgccc
     1321 ggccaaaggc ggcccgggcg gcatcgggct ggttgcgggc gatcagcagc agcccttcga
     1381 ggtggtcggg cagcgggccc ttgtcgtcgt tgcgcttgag cagcgcgatc accttggcgg
     1441 cctcgtcgag ctggccgctc tggattttgc tcagcgcggc catcgcgctg gcctgcgcca
     1501 gccgtggatc gctcttgagc agcgcttcca gatcttcgat gccctcatcg gtgcggccgg
     1561 cctgcaggtt ggccagcccc agccgcgcct gggctgccgg tgaattggcg ccctgcttga
     1621 gcgcggcttc gaggtggcgc aggccggcgg cgctgttgcc gcgcttcagc tccacctcgc
     1681 ccagcaggtt gagggcggcg acatcgtcgg gctgctgctt cagcagttca gtcagatttt
     1741 tggcggcgct gtcatgctgc tgggagcggt actggctcag tgccagcgcc agccgcgccg
     1801 ctgccgagcg tggcacgatc tccagcgcct gcttcaggta ctgttcggcc tgggccggct
     1861 ggttgcgcag caggtgggtg aggccgaggt agtagtggct ggtgaagtgg cggctgttgc
     1921 cgcgcaccgc tttttcgagc gcgctctgcg cctcgtcgaa gtggcgctgg cggaactggt
     1981 tcatcccctg caggtaggca accagtggat ggtcgccgag ctgcttgtgc agcagctgga
     2041 tgtcttcgtc ggcgccgctg gggttgttga ggacgatgcg cgccagcgcg cggtggtagc
     2101 ggtcgttgat caggttgggg cggcggcgca gcgcttcgct gtagctctgt tcggcggctt
     2161 cggtctggcc attgagccgc tgccactcgc ccttcaggct ccagagttcg gcgtcgtcgc
     2221 tgtcgatggc ggcggcgctg tcgatctcgc ggttgacggc ggcagcatca cccttgatgg
     2281 cggcgatgcg tgcgaggcca aggtgggcgc gggcggcgcg ggcatcctgc ttcagaatgt
     2341 cgcggtagcg ctgctcggcc gcatcgagcc gttcgccggc cagttcggca tcggcgcgca
     2401 gcagttgcag ccgcagcggt tcgctggcgc gggccgggtc gagctgttcg gccagcttca
     2461 gtgcctcctg ccagttgccg agcgccagca gcgcctcgcc aaaaccgacg ttgaccagtt
     2521 cggcggcggt gcccagcgca tgggcgcggc gcagctcttt ctcggcggcg tcgtagatgc
     2581 ccatctgcag atagaggttg ccgagcagca gccggccggc cgggtcatcg ggcgccagct
     2641 tgaccgcgtt gcgtgcctcg atgctggcct ccttgtattg cccctgttgc tgatagtgct
     2701 gggccttggc cagatattcc gcagcggtag gttcacggcc acagccggcg acaccggtca
     2761 gcaggacaac gagcgccaga cgggcgcagt gacggcggtt gttcggcact tcaggctcct
     2821 ttgaaagaat tggccgccgg cagggcatcg gtggcggcgc gggggcgatt tccacacgca
     2881 gtctcattat aatgcgctcc gtcgttttat ctgcgggtgc aggccagcca cagagcgaac
     2941 agagcagaac caaccggggc gctccccacc ttccacttgc caggcgttgc agagcgttat
     3001 gtccagaccc catgccttcg accgcgatgc gctgctgcag tgcggtcatg gcgaactctt
     3061 cggccccggc aacgcgcagt tgccggttcc caacatgctg atgctcgacc gcatcacgct
     3121 gatcaccgat gacagtggcg cccatggcaa gggcgagatc accgccgaac tcgacattcg
     3181 tcccgacctg tggttcttct cctgtcattt tcccggtgat ccggtgatgc cgggctgcct
     3241 ggggctcgat gcgatgtggc aactggtcgg cttctttctg gcctggcgcg gcaatcccgg
     3301 ccgcggtcgg gcgctgggct gcggcgaggt gaagtttttt ggtcaggtgc tgccgcaggc
     3361 gcagaaggtc acctatcaca tccagctcaa gcgggtgatc gaacgcaagc tggtgatggg
     3421 cattgccgat ggcaccatgg cggtcgatgg caccaccatc tacagcgccc gcgatctgcg
     3481 ggtcgggctg ttccagtcga ccgagcgcat gggctgaaac ggcagtttaa cgctgctggg
     3541 gtttgatcag gccgccccgg caccgtcgag tggccggggg cgcagtgccg tcccggccgg
     3601 caatcattcc aacaggcctt cactggaggt tccgcgatga tgcgtcgcgt ggtggttaca
     3661 ggtcttggca tcgtctcctg tctcggcaat gaccggcaga gcgtcaccga ttcactgcgc
     3721 gagggccgtt cgggcatcgc gcgcattgac gactatgtgc agatggggct gaagagccac
     3781 atcggcggca cgccgaaggt cgacttcgaa gccgccatcg atcgcaagct gctgcgcttc
     3841 atgggcgatg cggccgccta tgcctatctg gcgatggagc aggcgatcag cgatgcccgg
     3901 ctgctgccgc agcagatctc cgatccgcgg gtcggcctca tcatgggctc gggcggcgcc
     3961 tcgaccgcca accaggtcga agcggtcgat ctgatgcgcg acaaggggct gcggcgggtc
     4021 ggtccctacc gggtaacccg cacgatgggc agcacggtgt cggcttgcct cgccaccccg
     4081 tttcagatca agggggtcaa ctactcgatc tcctcggcct gcgccaccag cgcgcactgc
     4141 atcggcaacg cgatggagct gatccagctc ggcaagcagg acatggtctt tgccggcggc
     4201 gccgaagaga tctactgggc gatgacaatg atgttcgatg cgatgggcgc gctctcgacc
     4261 cagtacaacg accagccgca gcgggcgtcg cgcccctatg accgcgaccg cgacggcttc
     4321 gtcatcgccg gcggtgcggg ggtgctggtg ctggaggagc tggagcatgc ccagcgccgt
     4381 ggtgcaccga ttctggccga gctggtcggc tatggcgcca cctccgatgg ccatgacatg
     4441 gtggcgccct cgggcgaggg ggcggcgcgc tgcatgcatc aggcgctggc cggcgcccgg
     4501 cgtccggtcg actacatcaa tgcccatggc accagcacgc cggtgggcga tctgtgcgag
     4561 ctgggggcga tccgcgaggt gttcggcgac gaggcgccgc cgatcggctc gaccaagtcg
     4621 ctctctggcc actcgctcgg cgcggccggc gtgcaggagg cgatctacag cctgctgatg
     4681 atggagcaga actttctggc cgcatcggcc aacatcgaca acctcgaccc cggtgccgag
     4741 gggctgccga tcctgcgcac gcgccgcgat ggcgttgagc tcaacagcgt gctctccaac
     4801 agcttcggct tcggtggcac caacgcctgc ctgctgttcg agcgtttcga gggctgaaac
     4861 ccggcgcgcg ggcggccacc gggttgcccg cagccgtcac cccagcagcg cggcgaacgg
     4921 gtgaaagtcg atcagatcgc ccgccgtcac gcgctgtccg ggctccacca ccgccagacc
     4981 atctgcccag gccgccgaac gcagcacccc cgacccctgg ttggggtgcg gttcgagccg
     5041 gtcgtggccg gttgcatcac gcaacagccg ggcgcgcagg tattcgcgcc gcttgcccgg
     5101 ctggctgacg ctgaagccgg ccggcacccc gacccgcagc ggtgcgacct cgcgcacccc
     5161 ctgccggcgc agcagatagg gccggcagag gatgcagaag gtgaccagca cggcggccgg
     5221 attgcccggc agtgccatga tcggcgtcgt gccgatctcg ccgaacgcaa agggcttgcc
     5281 cggtttgatc gccagttgcc acagcttcag gcggccgagc tgctggatgg cgctgatcag
     5341 gtgatcctcc tcgccgaccg aggcgccgcc ggtggtgatc agcaggtcgg ccaccgcggc
     5401 ggcctgctgc agggctgcgc gcgtcgcggc cagcgtgtcg tcgacgatgc cgaaatcgtc
     5461 gatctggcag ccgagcccgc gcagcaaccc ggtcagcagg tagcggttgg agttgaacag
     5521 ccgcgcgccg ctcgccagct gctcggcccc gccctgaccc ggttccagca gttcgcggcc
     5581 ggtggagagc agggcgaccc gcagcggacg acagagcgga atctgcgcca cgccggccga
     5641 ggcgatcagc cccagctcct gtggacgcag acgggttccg gccggcaaca gcaggctgcc
     5701 ggctgcgatc tcgtggccgc gccggcggac attggccccc ggtcggggtg tcaccgtgaa
     5761 ccgcacctgg tctgcttcgg ggtcggcatc ctcctgcatc accaccgcgt cggcgccggc
     5821 cggcagcggc gcgccggtga agatgcgcgc caccgtgccg ggaaccagtt cggcagcgac
     5881 atcgccggca gcgatgcgtt gcgagatcgg cagtggccgg ccggcttcga cctcccggct
     5941 gcgcacggca tagccatcga ccgcgctgtt gtcgacagcg ggctggtcga acggggcgtg
     6001 caggtcgcgc gccagcaccc ggccgagcgc atcgagggtg gcgcccggtt gcactgccgg
     6061 ggcgccgtcg gcagccgcca gcagttgttc gatggcgaca tcgaccgggg tcagtggagt
     6121 gccacccatc gtcactggcc gccgctggtc gtgccgagca gatggccgac gaagctgcag
     6181 gggcgctggc ggctgtcgat ctgctcgcgg atgatccggt tccagccggt ctggcaggcg
     6241 ccggtcgagc cgggcagcac gaagatcagc gtgcggttgg cgagtccggc cagcgcgcgc
     6301 gactgaatcg tcgagctgcc gatctcttcc agcgagaagt ggcggaacag ctcgccaaag
     6361 ccatcgatgt gctgatcgaa cagcggtgcc agcgcctcgg gcgtgccatc gcgctgcgag
     6421 aaaccggtgc cgccggtgac cagcaccacc tgaatggccg gatcggcgat ccagaccgag
     6481 accgccgcgc gaatctggta gcggtcatcg ggcacgatgc gcttgtcccg cacgcgatgg
     6541 ccggcgtcgc gcgctgcctc ggcgaggaag cggcccgagc tgtcgctggc ctcatcgcgg
     6601 ctgtcggaga ccgtcagcac ggccagatcg aggggaacga attcggtggc ggcatgggac
     6661 atggcagggc tctcgcaacg aaacggggcg gccattgtcc gctgccgcac cgggtcgggc
     6721 aaccggccaa cgcttgatcc agatcaatgg cgaacagtga ccgcttgcct atagtccgcc
     6781 ctgcattcct caactttgga gatcagcatg aaaaaaagca tggcgatggc gacactctgt
     6841 ctgctcgcgg tcgggaccgg ctctgcctac gcaggtgagg ggccatggct ggtgcggctg
     6901 cgcgcactcg acatgaatgt cgacaacgag aacaacatca ccccggtgac cacggcctat
     6961 ggcgaggtcg atgtcgagga caagtggttt ccggagatcg acatcagcta cttcttcacg
     7021 cccaacatcg cggccgaact ggttctgacc tacccgcaga agcacgatgt gaagctcggc
     7081 aacttcgaca tcggcagcct caagcacctg ccgccgacgc tgctgctgca gtaccacttc
     7141 tgcccggaag ggacggttcg cccctatgtc ggtgccggca tcaactacac ccacttcagt
     7201 gatgtcgacc tgaacgccgc gccggcggtc gggacggatg cgccgctgcg gctcgacacc
     7261 agcagctggg gtctggcagc gcaggtgggg gtcgatttca aggttgcgcc caactggttc
     7321 gtcaacctcg atgccaagta tgtgcagatc gacgcgaacg acatccgggt gaagagtggt
     7381 gtcttggcgg gcacctacgt gaccgatctg acggtcaacc cctggctgct cagcgtcggc
     7441 gcgggctacc gcttctgaca ctccccttgg ggcgggccgg tcggctcgcc cccttttctt
     7501 ttcgcaatgc ggcgccttcg ctgaatccgt tgcacagcgc gagtgctctt tgctagattg
     7561 accggaacaa taacaggcga ggatggcgct gccgccatca ctcgaccggg gaatcagcca
     7621 gaggattggc gaacatgatg acccagccgg ctggattgac cgcacagatc gccgcccagg
     7681 cggcccaccg caccttcgag catctaccgg ccagcatggt tgcgctggcg aagcagtgtc
     7741 tgctcgactg gctgggcgtc acgctggcgg gcagccgtga acccctggtg gagacgctgc
     7801 tggcgcagag ttgcagtgaa ggtggccgtc cgcaggcgac ggcagtgggc cacgcaccgc
     7861 gcgtgaaccg gctgcaggcg gcgctgctca atggcacggc ttcacatgcc ctcgactacg
     7921 atgacgtcaa catggcgatc attgggcatc ccaccgcgcc ggtgctgccg gcagcct
//
LOCUS       DAORCQ010000103         7837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6542_length_7837_cov_6.095991, whole genome shotgun sequence.
ACCESSION   DAORCQ010000103 DAORCQ010000000
VERSION     DAORCQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6542_length_7837_cov_6.095991"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /gene="crcB"
                     /locus_tag="PKI23_09765"
     CDS             complement(<1..354)
                     /gene="crcB"
                     /locus_tag="PKI23_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNN37261.1"
                     /translation="MNPWLAVAVGGALGSMARFAVGTWVAAGLGSRFPYGTLLVNLLG
                     CLLMGLLVVLIDERWAVPPQWRAFALVGLLGGFTTFSSFSLEAYTLLRTQQWLAALLY
                     VAGSLLLGLLALAVGI"
     gene            620..1372
                     /locus_tag="PKI23_09770"
     CDS             620..1372
                     /locus_tag="PKI23_09770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN37262.1"
                     /translation="MVAPHPSSPSLSLSVVIPVKDERANIAPLAAEIVAALDGRCTFE
                     AIFVDDGSSDGTDDEIHHAIARHAGHVRGIRLARNCGQSTAVHRGVGAARYPWVATLD
                     GDGQNPPAEINRLLAALQNSGSDPLLLVAGCRLQRNDDWIRRLSSRVANGVRRRMLRD
                     DTPDTGCGLKLFARDTFLQLPYFDHMHRFLPALYQRLGGRVCCVEVNHRPRAAGRSKY
                     GIHNRLWVGIVDLFGVAWLQRRARLSPMSDIE"
     gene            1369..1716
                     /locus_tag="PKI23_09775"
     CDS             1369..1716
                     /locus_tag="PKI23_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012272480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNN37263.1"
                     /translation="MSIEAMLAPIIGFFHQSDPLWLLVGFAGQLLFSMRFIVQWLQSE
                     RVKRSVVPTAFWYFSVAGGATLLGYAIHRRDPVFILGQGIGLFIYFRNLQLIYRHGTA
                     DTDSLPATPGQHH"
     gene            1753..3357
                     /locus_tag="PKI23_09780"
     CDS             1753..3357
                     /locus_tag="PKI23_09780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNN37264.1"
                     /translation="MSRAPLTLPVMLLLWSLLWLLGVSIRPPFPIDETRYLTVAWEMY
                     QRNDWLLLHLNGTPYSHKPPLLFWLINLGWGVFGVNGWWPRLVVWLCALLGLLGTHRL
                     ARRLWPTRPQIALTSTLLLGGCSYWLLFSHLLMFDLILSAWVALGLNALLRTLHGERA
                     GWLLLAIATALGGLTKGPVILLHLGLAALVMRLTLHEPASPRQWLPKALATTIVGLLP
                     LLLWALLAAHRGGDAFAHELLFKQTGERMVSSFAHRRPFWWLLAMLPLLLLPWTLSLT
                     AWRAAKGLPRLWSEPGLRFAALWSLAVLAAFSLISGKQPHYLLPIFPALALLLARAID
                     ELPQSGAPQREWLLAGMLLLFALSIMLITTGRLANPQLQALAPLLPGWLAAAFALLAL
                     LCLPGLDRHRSTLRNSLVIGGMTALLYLGLLPAQLPRYRADDVSALLHEAEQQQIPVA
                     FIDFEYDGQFTFPALLRQPLAVVPAAQAHGWAAQHPDGWLLFILDEARAADSQPRPLL
                     ERRYRGSMLYLYSAQALLEQGEFPSD"
     gene            3452..5038
                     /locus_tag="PKI23_09785"
     CDS             3452..5038
                     /locus_tag="PKI23_09785"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNN37265.1"
                     /translation="MAGHRNLLITLTLAALPALPLQAAPPDPADDSMMQGRNGARVTP
                     LFTVGETVTPDDGGEGYQPVGVLDGLGAYRLDADTVRLWVNHELADDKGRPYRLANGT
                     LLRGARISQFDIDRHSRTLRRAGIAYAEVHDRAGQPVTRAEQINGPGSHGFDRFCSAA
                     LFEPNRFGEDRGFRDRIHFAGEERSRSPGGTLWAGEVDRGRLWAVPMLGRGAWENVAE
                     IDTGTPERIALLLGDDHVGAPLYLYVGIKNGDPHSSFLDRNGLQQGRLYAWKSAGNDA
                     DPATFHGMAAQRDGHWVELHPRDPARASQPGHDALGDVDAETLRAEADRLGAFSFSRP
                     EDLHVNPRNGRQAVLASTGRADFAGGSDTQGDLYLIDLQFDAQGNPGPARLTLLYDGD
                     ADPALRLRSPDNLSWSSDGRILVQEDAAYPWQPQQNPAEARIVAIDPLTLTLTTLAEI
                     HRAAVRPSGSSDAKAKELGAWESSGIIEVSSLFDAPPGTLHLFDVQAHGIRDGMIGER
                     DLVEGGQLLWLEVGDPVSRR"
     gene            complement(5046..5774)
                     /locus_tag="PKI23_09790"
     CDS             complement(5046..5774)
                     /locus_tag="PKI23_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37266.1"
                     /translation="MRRYLAGSMMAVALWSVQSDAALIYSNNFDTPAFKAAGVSASLT
                     GGFVGAVSAGPYAGSLGKSWAGNYFNGNGASATLTLTGLPVHTGVNVDLLLGFLNSWD
                     SRNGTVARDDLNLYIDGRLLLSMTTNNASGTVVDFDGGTLLVNDGQIDSNRFFSDDLV
                     DMATASLLTLAHTAPSLTLRITPTGRGWQGWPDEGWGMDALRVSVTGDESTHGVPVGP
                     TLSLFGIGLAGLGLARWHGARRRG"
     gene            5956..7698
                     /locus_tag="PKI23_09795"
     CDS             5956..7698
                     /locus_tag="PKI23_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNN37267.1"
                     /translation="MRTPSRLWPLLLLCCLCSAALRAETDLPVRVELSGIDTTARDNI
                     LATLTIAQQGDRALSPARVRLLHRRAGQQIIDALRPLGYYRATVDGAVERDARQWQAR
                     YRITPGAPLPIGQVDLRFTGAAEQDEAMLRLQRELPLKSGEPLRHDQYEAAKKQIQRL
                     AAERGYFTARFTRHQVRVDLDRYQADIQLWFDSGPRHRLGPVRFSDSRISPELLRRYL
                     RQAPGTPYRQSALLRLQERLVDSGYFSQVEVVAQPERAQNDEVPVEVKLFDRKPDRFQ
                     IGAGYGTDTGARMRGSYEYQPINPQGHRFDSEAVLSQLNQKIDARHLIPLARPDRDQF
                     ITHAGWENDQPPNDSESAAFKFGVGVQQGLGQWRRLIGLDYQSERFQIGDEEGLSQLL
                     MPTLGLTRTQADHPIHVTHGNSLQLSLRGSIEGTLSDATFVQASLKGKRIDPLGEGRL
                     LSRLELGSSWVDEFDQLPPSVRFFAGGDQSVRGHDYKALGPEDADGEVLGGKHLLVGS
                     VEYEHPFNDDWRGAIFIDAGNAFDRFDQPLHRGVGLGLRWKLPVGWVRIDLAHPHPFA
                     EGGWRLHFTMGPDL"
BASE COUNT         1269 a         2780 c         2504 g         1284 t
ORIGIN      
        1 gatgccgacc gccagcgcca gcagccccag cagcaggctg ccggccacat agagcagggc
       61 cgcgagccac tgctgggtgc gcagcagggt gtaggcctcg agcgaaaagg aggagaaggt
      121 ggtgaagcca cccagcagcc ccaccagcgc aaaggcccgc cactgcggcg gcactgccca
      181 gcgctcgtcg atcagcacca ccagcagccc catcagcaga cagccgagca gattgaccag
      241 cagcgtgcca tagggaaagc ggctgccgag cccggccgcg acccaggtgc cgaccgcgaa
      301 acgggccatc gaaccgagcg cgccgccgac cgccacggcc agccacgggt tcatgtcgcg
      361 ctccacgcag cgacccgacg ggcggagcgg tcggccgatg gcgggagaga gggcagatga
      421 ggcaagcgcg gccactccag gcagtgaggg tggcgtcgat tataccgatt cgcgcgcact
      481 tcaccgctgc ggttttcacc cggccaacga ccgcaccacc cgccatggct gacagccgga
      541 tcgggtttcg gagtacaatg ccggcgccct gctgcacgca gcagggcgtt cctcatcacc
      601 ctgtcacctg ccactgccaa tggtcgcccc gcatccatcc agcccgagcc tgtcgctgtc
      661 tgtggtcatt ccggtcaagg atgagcgggc caacatcgcc ccgctggcgg ccgagatcgt
      721 cgccgcgctc gatggccgct gcaccttcga ggcgatcttt gtcgacgatg gcagcagcga
      781 tggcaccgac gacgagattc accatgccat cgcgcgccac gccggccatg tgcgcggcat
      841 ccggctggcc cgcaactgcg gtcaatcgac cgccgtgcac cgtggtgtcg gcgcagcccg
      901 ttacccctgg gtcgccacgc tcgatggcga tggccagaat ccaccggccg agatcaaccg
      961 gctgctggcc gcactgcaga acagtggcag cgacccgctg ctgctggtgg ccggctgccg
     1021 gctccagcgc aatgatgact ggatacgccg gctctcctcc cgggtcgcca acggtgtgcg
     1081 ccggcgcatg ttgcgtgacg acacccccga caccggctgc ggcctcaagc tgttcgcccg
     1141 cgacaccttt ctgcaactgc cctacttcga ccacatgcac cgctttctgc cggcgctcta
     1201 ccagcggctg ggcgggcggg tctgctgtgt cgaggtcaac caccggccac gcgccgcggg
     1261 ccgctcgaag tacggcatcc acaaccggtt gtgggtcggc atcgtcgacc tgttcggcgt
     1321 cgcctggctg caacggcgcg cccggctctc gccgatgagc gacatcgaat gagcatcgaa
     1381 gcaatgctgg caccgatcat cggcttcttt catcagagcg atccgctctg gctgctggtg
     1441 ggattcgccg gccagctgct gttctcgatg cgcttcatcg tgcagtggct gcagagcgaa
     1501 cgggtcaagc gcagcgtggt gccgaccgcc ttctggtact tctcggtcgc cggcggcgcc
     1561 acgctgctcg gctatgcgat tcatcgccgc gacccggtct tcattctcgg ccagggcatc
     1621 ggcctcttca tctattttcg caatctgcaa ctgatctacc gccacggcac agcggacacc
     1681 gactccctgc ccgccacacc cggccaacac cactgatcgg ctgattcccg ctccaccgtg
     1741 caagagttcc gcatgagccg cgcgcccctc accctgcctg tgatgctgct gctctggtcg
     1801 ctgctgtggc tgctgggggt gtcgattcgc ccgccgttcc cgatcgatga gacccgctac
     1861 ctcaccgtcg cctgggagat gtaccagcgc aacgactggc tgctgctgca cttgaacggc
     1921 accccctaca gccacaagcc gccgctgctg ttctggctga tcaacctcgg ctggggagtg
     1981 ttcggcgtca acggctggtg gccgcgcctc gtcgtctggc tctgtgcgct gctcggcctg
     2041 ctcggcaccc atcgactggc gcgccggctc tggcccacac gcccgcagat cgccctgacc
     2101 agcacgctgc tgctcggcgg ctgcagctac tggctgctgt tttcccatct gttgatgttc
     2161 gatctgatcc tgtcggcctg ggtggcgctg gggctcaacg cgctgctgcg cacgctgcac
     2221 ggcgaacgtg ccggctggct gctgctggcg atcgcgacgg cactgggcgg gctgaccaag
     2281 gggccggtga ttctgctgca cctcggcctg gccgcgctgg tgatgcggct gacgctgcat
     2341 gaaccggctt cgccacgcca gtggctgccg aaggcgctgg cgacgaccat tgtcggtctg
     2401 ctgccgctgc tgctgtgggc gctgctggcg gcccatcgcg gcggtgatgc ctttgcacac
     2461 gagctgctgt tcaagcagac cggcgagcgg atggtctcct ccttcgccca ccgccgaccg
     2521 ttctggtggc tgctggcgat gctgccgctg ctgctgctgc cctggaccct cagcctgacc
     2581 gcctggcgcg cggccaaagg gttgccacgg ctgtggtccg agcccggtct gcgctttgcg
     2641 gctctgtgga gtctggcggt gctggcggcc ttctcgctga tcagcggcaa gcagccacac
     2701 tatctgctgc cgatctttcc ggcgctggcg ctgctgctgg cccgcgcgat cgatgaattg
     2761 ccgcagagcg gtgccccgca acgcgaatgg ctgctggccg gcatgctgct gctcttcgcg
     2821 ctgtcgatca tgctgatcac gacgggtcgg ctcgccaatc cacagcttca agcgctggcg
     2881 ccgctgctgc ccggctggct ggcggccgcc ttcgcgctgc tggcgctgct ctgtctgccc
     2941 ggtctggatc gccaccgctc cacattgcgc aacagcctgg tcatcggcgg catgaccgcc
     3001 ctgctctacc tcggcctgct gcccgctcaa ctgccacgct accgtgccga tgatgtcagc
     3061 gcactgctgc acgaggccga acagcagcag attccggtcg ccttcatcga cttcgaatac
     3121 gacggccagt tcacctttcc ggccctgctg cgccagccgc tggcggtggt tccagccgct
     3181 caggcacacg gctgggcggc acagcacccc gacggctggc tgctgttcat tctcgatgag
     3241 gcccgcgccg ccgacagcca gccgcgcccg ctgctggagc gccgctaccg cggttcgatg
     3301 ctctacctct actccgccca ggcactgctc gaacagggcg aatttccatc cgactgaccc
     3361 ggtgctggca gagattggag cgaatgaaga gaatctgtca tcgatcgacc gaacaatggc
     3421 cactgcccca ttcaccccca ggaggcacac catggccggc caccgcaacc tgctgatcac
     3481 actgaccctc gctgccctgc ccgcgctgcc gctccaggcc gcaccgccgg accctgccga
     3541 cgacagcatg atgcagggcc gcaacggtgc ccgggtcacg ccgctgttca ccgtcggcga
     3601 gacggtcacg ccagacgatg gcggagaggg ttaccagccg gtcggggtgc tcgacggact
     3661 gggcgcctat cgcctcgatg ccgacacggt gcggctgtgg gtcaatcatg aactggccga
     3721 tgacaagggc cggccctatc gactcgccaa cggcaccctg ctgcgcggtg cgcgcatcag
     3781 ccagttcgac atcgaccgcc acagccgcac gctgcgtcgc gccggcatcg cctacgccga
     3841 ggtgcatgac cgcgccggcc agccggtcac ccgcgcggag cagatcaacg gccccggcag
     3901 ccacggcttc gaccgcttct gctcggccgc actgttcgag ccgaaccgct tcggcgagga
     3961 ccgcggcttt cgcgaccgca tccatttcgc cggcgaagag cgcagccggt caccgggcgg
     4021 aacgctctgg gccggcgagg tcgaccgcgg ccgcctgtgg gccgtgccga tgctcggccg
     4081 tggcgcctgg gagaatgtcg ccgagatcga caccggcacc ccggagcgga tcgccctgct
     4141 gctgggcgat gaccatgtcg gcgcgccgct ctatctctat gtcggcatca agaacggtga
     4201 tccgcacagc agctttctcg accgcaacgg cctgcagcag ggccggctct atgcctggaa
     4261 gtcggccggc aacgacgccg atcccgcgac cttccatggc atggcggcgc agcgtgacgg
     4321 ccactgggtc gagctgcatc cacgtgatcc ggcccgggcc agtcagccgg gtcatgacgc
     4381 cctgggcgat gtcgatgccg agaccctgcg cgccgaggcc gatcggctcg gcgccttcag
     4441 cttctcgcgc cccgaggatc tgcatgtcaa cccccgcaat ggccggcagg cggtgctcgc
     4501 ctccaccgga cgcgccgatt tcgccggcgg cagcgacacc cagggcgacc tctatctgat
     4561 cgatctgcaa ttcgacgcgc aaggcaaccc cggcccggcc cgcctgaccc tgctgtacga
     4621 tggcgacgcc gacccggcgc tgcggctgcg cagcccggac aacctcagct ggagcagcga
     4681 cggccgcatc ctggtgcagg aggacgccgc ctacccatgg caaccgcagc agaatccggc
     4741 agaggcgcgc atcgtcgcca tcgatccgct caccctaacc ctgaccaccc tggccgagat
     4801 ccaccgcgcc gcggtccggc ccagcggcag cagcgatgcc aaagcgaagg agctgggcgc
     4861 ctgggagagt tcgggcatca tcgaggtgtc gtcactgttc gatgccccac ccggcacgct
     4921 ccatctgttc gacgtgcagg cccacggcat ccgtgacggc atgatcggcg agcgggatct
     4981 ggtcgaaggc ggacagctgc tctggctcga ggtgggcgat ccggtcagcc gccgctgaaa
     5041 cgcaatcatc cccggcgcct ggcaccatgc cagcgcgcca gacccaaccc cgccagcccg
     5101 atgccgaaca gcgagagcgt cggtccgacc ggcaccccat gggtcgactc atcgccggtc
     5161 actgacaccc gcagcgcatc catgccccag ccctcgtccg gccagccctg ccagccgcga
     5221 ccggtggggg tgatccgcag cgtcaggctc ggcgcggtgt gggcgagcgt caacagcgag
     5281 gcggtcgcca tgtccaccag atcatccgaa aaaaagcggt tggaatcgat ctggccgtcg
     5341 ttcaccagca aggtgccgcc atcgaaatcg accaccgtcc cggaggcatt gttggtggtc
     5401 atgctcaaca gcagacgccc atcgatgtag aggttcagat catcccgcgc cacggtgcca
     5461 ttgcggctgt cccaggagtt gagaaagccg agcagcagat cgacgttgac cccggtgtgt
     5521 actggcagac cggtcagcgt cagcgtggcc gaagcgccat tgccattgaa gtaattgccg
     5581 gcccagcttt tgccgagact gccggcatag gggccagcgg agacagcgcc gacgaagccc
     5641 ccggtcaggc tggccgagac acccgcggcc ttgaaggccg gcgtgtcgaa attgttcgaa
     5701 tagatcagcg cggcatcgga ctggacgctc cagagcgcaa ccgccatcat cgaacccgcc
     5761 agatatcgcc gcatgagaac ctcttttgcc atggtgaaaa aaacgggcgg cacaatcgcg
     5821 caacatgcag ccgcatgagc gacctgcgcc ttcccggact ggcgcagcca tcgagctgca
     5881 catggctgta aaactagatg gaatttcagg ttgttccact cttcccaccg ccgcagttgc
     5941 gtcggcagct cgacattgag aacaccttct cgcctctggc cactgctgct gctctgctgc
     6001 ctctgctcag ccgcgctgcg ggccgagacc gatctgccgg tccgcgtcga actgagcggc
     6061 atcgacacca ccgcacggga caacatcctg gccacactga cgatcgccca gcagggtgat
     6121 cgcgcgctct cgccggcacg ggtcaggctg ctgcaccgcc gtgccggaca gcagatcatc
     6181 gatgcgctgc gtccactcgg ttactaccgc gccacggtcg atggcgcggt cgagcgcgac
     6241 gcccggcagt ggcaggcccg ctaccggatc acccccggcg cgccgctgcc gatcggtcag
     6301 gtcgatctgc gcttcaccgg cgcggccgaa caggacgagg cgatgctgcg cctgcaacgt
     6361 gagctgccgc tgaaaagcgg tgagccgctg cggcacgacc agtacgaggc ggccaagaag
     6421 cagatccagc ggctggcggc cgagcggggc tacttcaccg cccgcttcac ccgccaccag
     6481 gtgcgggtcg acctcgatcg gtatcaggct gacatccagc tctggttcga cagcggtcca
     6541 cgccatcgcc tgggtccggt ccggttcagc gattcgagaa tttcgcccga actgctgcgc
     6601 cgctatctgc ggcaggcgcc aggcaccccc tatcgccagt cggcgctgct gcgcctgcag
     6661 gagcggctgg tcgacagcgg ctacttcagt caggtcgagg tggtcgcaca accggagcgg
     6721 gcgcagaacg acgaggtgcc ggtcgaggtg aaactgttcg accgcaaacc ggacaggttc
     6781 cagatcggtg ccggctatgg caccgacacc ggcgcccgga tgcggggcag ttacgaatac
     6841 cagccgatca atccgcaggg gcatcgcttc gacagcgagg cggtgctgtc gcaactgaac
     6901 cagaagatcg atgcccgcca tctgattcca ctggcccgcc cggaccggga tcagttcatc
     6961 acccatgccg gctgggagaa cgaccagcca cccaacgaca gcgagagcgc cgccttcaaa
     7021 ttcggcgtgg gcgtgcagca gggtctgggg cagtggcgcc ggctgatcgg cctggattat
     7081 cagtccgaac ggttccagat cggcgacgaa gagggactct ctcagctgct gatgccaacg
     7141 ctcggcctga cccggaccca ggccgaccac ccgatccatg tgacccacgg caacagcctg
     7201 caactgtcgc tgcgcggcag catcgagggg acgctgtccg atgccacctt cgtgcaggct
     7261 tcactcaagg gcaagcgcat cgatcccctc ggcgaaggcc ggctgctgag ccgcctcgaa
     7321 ctgggcagca gttgggtcga tgagttcgac cagctgcccc catcggtgcg gtttttcgcc
     7381 ggcggtgacc agagcgtgcg cggccatgac tacaaggcgc tgggaccgga agatgccgat
     7441 ggcgaggtgc tgggcggcaa gcatctgctg gtgggcagcg tcgagtatga acatccgttc
     7501 aacgacgact ggcgtggtgc gatcttcatc gatgccggca atgccttcga ccgcttcgat
     7561 cagccgttgc atcgcggtgt cggcctgggt ctgcgctgga agctgccggt cggctgggtc
     7621 cggatcgatc tggcccatcc gcaccccttt gccgagggcg gctggcgact ccacttcacg
     7681 atgggacccg atctgtgaag cgcctgctgc gctgggtgtt ttcactctcg gccctgctgt
     7741 cgctgtgcgc cctcggctgg ctgctgctga gtccggcagc gccaccgctg ttgtggcgca
     7801 ccctgcactg gctgctgccc ggtgaactgc aggtggc
//
LOCUS       DAORCQ010000104         7802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6582_length_7802_cov_5.819801, whole genome shotgun sequence.
ACCESSION   DAORCQ010000104 DAORCQ010000000
VERSION     DAORCQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6582_length_7802_cov_5.819801"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2207
                     /gene="pepN"
                     /locus_tag="PKI23_09800"
     CDS             <1..2207
                     /gene="pepN"
                     /locus_tag="PKI23_09800"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010793218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNN37268.1"
                     /translation="RFPVLLSNGNPVAHGSLADGRHWVTWDDPFRKPCYLFALVAGRL
                     DHHASQFHTRSGRTVQLRIHVEPKDLDKCAHAMASLQQAMAWDEQHYGCEYDLDQYNI
                     VAIDDFNMGAMENKGLNIFNTSCVLAHPQTTTDAAFARVAAVVAHEYFHNWSGNRVTC
                     RDWFQLSLKEGFTVFRDQQFSADLGSATVKRIEDVNLLRTVQFAEDSGPMAHPIRPDS
                     YLEISNFYTPTVYEKGAEVVRMLHQLLGAQPFRRGCDHYFSQHDGEAVTTDHFVSAME
                     AANDVDLSQFRRWYSQAGTPIVRVEDAYDAESRRYTLTISQSTPSTPGQPGEAKQPLV
                     IPLSLALYDGQGREMPLQCDDEADAATTQRVFAITESRQQLRFRNVAERPTPSLLRGF
                     SAPVRLEYDYSRDALRFLARHDSDGFNRWEAMQRLMVLCIQEQIASHLRTETTPFTAD
                     LIETVQALLDDQQLTPAELARMVQLPSEAYLSGLAEPIEVEAIHAARRGWQRELGQQL
                     IGRWQQLFEANRPAPPYRADAEQSGRRALRAVALEYLAAADVSGAEEASRLYFAADNM
                     TEALSGLRTLLHSPYPELEALREQALSHFYQRWQHEPLVVDQWLALQATAPRPETLQR
                     VETLLMHPAFDLRNPNKSRALIGSFAGQNPIQFHRTDGRGYRFLADQVLRIDPLNPQI
                     AARLVLPLTRWKKYGTTRQTTMRAELARIAEGTTLSRDLLEVVSKSLASEAV"
     gene            complement(2213..3472)
                     /locus_tag="PKI23_09805"
     CDS             complement(2213..3472)
                     /locus_tag="PKI23_09805"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNN37269.1"
                     /translation="MERVLPEQLKLFSSFSSCDDAWLSALSDQLSLRSAPAKTVLLNL
                     GSTERGELFLVEGTLALEARDGRRSVIKSGSESAALPIARLRPSMYRVVALTPVRFLW
                     VAPNLLRPAKEPEPAMQVEFSVQTANELARIEVQQDATTGSLYFDIYEDLKNDALALP
                     TLPVVALRIREMIDSEQSNAEMVAKAAASDPAIAAKLLRISNSPLYRGQSHFETLTQA
                     IVRLGMQSTKQIVTSFAVRELFTSDQPLLKQRLMALWQHSVEVAATCFVLARLNRGLN
                     PEQALLTGLLHDIGVLAIISHAEHYPDITATPERLESVIHEFGRRVSVLILQKWDFPA
                     AMVEACQKCESWSADNGAQLTYADLVVLAQVHLLRGHPDYPQLPDIQTLPAYRKLSNQ
                     GLTSAGLLKILDEASEQVDEARQLLLA"
     gene            complement(3551..3880)
                     /locus_tag="PKI23_09810"
     CDS             complement(3551..3880)
                     /locus_tag="PKI23_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNN37270.1"
                     /translation="MDELRKSPRITLDQELQVIDRGDGSRMGVVVNVSQGGVMVAGDR
                     PVVTDRLYRLRLELTTPVAGKTHIHLGADALWSQNAEGSQYYTGFQLIDIADDDLVLL
                     LESAAKF"
     gene            complement(3937..6360)
                     /locus_tag="PKI23_09815"
     CDS             complement(3937..6360)
                     /locus_tag="PKI23_09815"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HNN37271.1"
                     /translation="MHFSQRQLIDQLRSEVISQVTSAVQRTFEQIEEKVFEKAEQARS
                     NQEQAAVFDDLREIRFRWPMVTEHFLEGVSEQINRFARNQPLPPIGGRPAATGGGLSL
                     VDEHVLNEEIIIGNMVQSANAQFHQSIHALNQRLAILSQGRKLEECDNPFGPERLGEA
                     CRGAVRLLNLSEEIRQLVLKSFGTEVLERLGRLYDRCNDLLIEADILPNLAFEVRRLH
                     GASGSSQRPTTEEKIEPSDSLQERVPQPSGFDAHPTSPSELFAQVQSMLAQPLPLVQE
                     PMATGAVAHPAMDPALAYSTGGVPMPAVQFMPFVPSAMAAVPGGGQSAAMAGAPPLMS
                     APSLRSMGSMRPDGVALVGDGASYTPHQLAQTVDQIQHHQYQSAAQVDHAQLLTVEAV
                     KRQLLEQVEKLNSIAGEVRQVATVDADLIDLVGMLFEYMLNDENLPDSVKALLSHLHT
                     PFLKVALLDRQFFTRSSHPARRLLNAMAKAGGHWLIDDDQEHGVFAKMRSIVDRILLE
                     YTLEIELFDEVLADFNQFIEKLEKRADLAEKRAMETLQGQEKLRTARNRAVQEVMSRT
                     EGHDLPEAVTRFLEQPWVDILVFTLLRKGTESASWHNTLKVVDDVIWSVTPKVSEEEK
                     SALRRQLPELNEAIQQGFVLLGGYQGEAKQLLRELAAAQSRALEARAEQVAVQQPVAE
                     KPPGQRNRVRAAEASSTGNLSPELQQAIAELRKVPFGTWFEFTINAQGDKKRGKLSWF
                     SPLTSRYMFVDQNGKQVGVRSLISLANSLLSGDAKVIRQAEEPLIDRAMSTIHDLLSR
                     RLRRRAGAV"
     gene            6548..7027
                     /locus_tag="PKI23_09820"
     CDS             6548..7027
                     /locus_tag="PKI23_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNN37272.1"
                     /translation="MPPSSYPPAGLLRRLGAMLYDTLLVVALLMCITAAYIFIALQLQ
                     GDTLAAGKVAARGALYQSILLISTFGFFAYFWTRHGQTLGMQAWRLRIQNSDGSTIRL
                     PQAVLRFFGALFSLLCLGLGYGWMLVDRDRMTWHDRLSSSLVVQLPKPEKKRKKRGG"
     gene            complement(7031..>7802)
                     /gene="lptG"
                     /locus_tag="PKI23_09825"
     CDS             complement(7031..>7802)
                     /gene="lptG"
                     /locus_tag="PKI23_09825"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNN37273.1"
                     /translation="GIAWSACRPVLLFMVATTLLGEWVAPAAEQAAQNWRAQLNGDGH
                     GRAGEGTWLRDGPDFVYFSAITAQGDLLGVTRYQFDQERQLVRLSHAQRASYAEGRWQ
                     LDQVAATWLTAEQSQSEQLAQQAWPSTLTPALLQVLALKPDRLSAIELYRYAVHLDQQ
                     RLESAPHWLAFWKRLFQPAATLALVLIGISFVFGPLRELTMETRVFAGIAVGVVFHFA
                     QNILGPASIVFQFSPIWAAALPPLLCLLFALRWLRRAG"
BASE COUNT         1529 a         2580 c         2223 g         1470 t
ORIGIN      
        1 aacgttttcc ggtgctgctg tcgaacggca atccggtggc tcatggcagc ctcgccgatg
       61 gccgccactg ggtcacctgg gatgatccct tcagaaaacc ctgctatctc ttcgcgctgg
      121 tggcgggacg gctcgatcac cacgcgtcac agttccacac ccgctccgga cgcacggtgc
      181 aactgcgcat ccacgtcgaa cccaaggatc tcgacaagtg cgcccatgcg atggcttcac
      241 tgcaacaggc gatggcctgg gatgagcagc actacggatg cgagtacgac ctcgaccagt
      301 acaacatcgt cgccatcgac gacttcaaca tgggtgcgat ggagaacaag gggctcaaca
      361 tcttcaacac ctcctgtgtg ctcgcccatc ctcagaccac caccgatgcc gcctttgccc
      421 gggttgcagc agtggtggcc cacgaatatt tccacaactg gtcgggcaat cgggtcacct
      481 gccgcgactg gttccagttg agtctcaagg agggattcac ggtctttcgc gaccagcagt
      541 tctccgctga cctcggctcc gcgaccgtga agcggatcga ggatgtcaac ctgctgcgca
      601 cggtgcagtt tgccgaagac agcggcccga tggcccatcc gatccgcccc gacagctacc
      661 tggagatctc caacttctac accccgaccg tttatgaaaa gggtgccgag gtggtgcgga
      721 tgctgcacca gttgctgggc gcgcaaccat tccgccgcgg ttgcgaccac tacttcagcc
      781 agcacgatgg cgaagccgtc accaccgacc atttcgtcag cgcgatggag gcggccaacg
      841 atgtcgacct gagtcagttc cgccgctggt acagccaggc tggcacgccg atcgtgcggg
      901 tcgaggatgc ctacgatgcc gaatccaggc gctacaccct caccatcagc cagtcgaccc
      961 cctcgacacc gggacaaccg ggcgaagcca agcagccgct ggtcattcca ttgagtctgg
     1021 ccctctatga cggtcagggt cgggagatgc cactgcagtg cgatgacgaa gccgacgcgg
     1081 ccaccaccca gcgggtcttc gccatcactg aatcccgaca gcagctacga ttccgcaatg
     1141 tcgccgagcg tcccacaccg tcactgctgc gcggcttctc ggcgccggtc aggctcgagt
     1201 atgactacag tcgcgatgca ttgcgctttc tcgcccgcca tgacagcgac ggtttcaacc
     1261 gctgggaggc gatgcagcgg ctgatggtgc tctgcataca ggagcagatc gccagccatc
     1321 tgcgcaccga gacaacccct ttcaccgctg atctgatcga aaccgtgcag gcactgctcg
     1381 atgatcagca gttgacgccg gccgaactgg ccaggatggt gcaactgccg agcgaggcct
     1441 acctgagtgg actcgccgag ccaatcgaag tcgaggcgat tcatgccgcc cgccgtggct
     1501 ggcagaggga actcggccag caactcattg gccgctggca gcaactcttc gaggcgaacc
     1561 gcccggcacc gccctatcgc gccgatgccg aacagagcgg tcgccgcgcg ctgcgcgcgg
     1621 tggcactgga gtatctggcg gccgccgacg tctctggggc cgaggaggcg agccgactct
     1681 acttcgccgc cgacaacatg accgaagcac tcagcggcct gcgcacactg ctgcacagcc
     1741 cctatccaga actcgaggcg ctgcgcgagc aagctttatc ccacttctat cagcgctggc
     1801 agcatgagcc gctggtggtc gaccaatggt tggcactgca ggccacggcg ccacgaccgg
     1861 agaccctgca acgggtcgag acgctgctga tgcatcccgc tttcgacctc cgcaacccga
     1921 acaagagccg ggcgctgatc ggcagctttg ccggtcagaa cccgatccag ttccatcgca
     1981 ccgatggccg cggttaccgc tttctggccg atcaggtcct gcgcatcgac ccgctcaacc
     2041 cacagatcgc cgcccggctg gtgctgccgc tgacgcggtg gaaaaagtac gggacgacac
     2101 ggcagacgac catgcgagcg gagctggcgc gcatcgccga gggtacgaca ctctcaaggg
     2161 atctgctcga ggtggtcagc aagagcctgg ccagcgaagc ggtctgatct gatcaggcca
     2221 gcagcagttg tctggcctcg tcgacctgct cgctggcctc gtccagaatc ttgagcaacc
     2281 cggcgctggt cagcccctgg ttgctcagct tgcggtaggc cggcagcgtc tggatgtcgg
     2341 gcagttgcgg atagtcagga tgaccacgca gcaggtgcac ctgtgccagc accaccagat
     2401 cggcataggt cagttgtgcg ccattgtcgg cggaccagct ttcacacttc tgacaggcct
     2461 cgaccatcgc tgccgggaaa tcccacttct gcaggatcag cacgctgacc cgccggccaa
     2521 actcatggat cacgctttca agccgctccg gcgtggcggt gatgtcggga tagtgctcgg
     2581 catggctgat gatggccaac acgccgatgt cgtgcagcag accggtcagc agcgcctgtt
     2641 cgggattgag cccccggttg aggcgggcca gcacgaaaca ggtcgcggcc acctcgacac
     2701 tgtgctgcca cagcgccatc aatcgctgct tcagcaaggg ttgatcactg gtgaagagct
     2761 cgcgcaccgc aaaactggtg acgatctgct tggtcgactg catccccaga cgcacgatgg
     2821 cctgcgtcag ggtctcgaaa tgtgactgcc cacggtaaag cgggctgttg gaaatccgca
     2881 gcaacttggc ggcaatggcc ggatcggagg cagccgcctt ggccaccatc tcggcattgc
     2941 tctgctcgct gtcgatcatc tcgcggatgc gcagcgccac caccggcaag gtcggcagcg
     3001 ccagcgcgtc atttttcaaa tcttcataga tgtcgaaata gaggctgccg gtcgttgcat
     3061 cctgctgcac ctcgatcctg gccagctcat tcgcggtctg taccgagaac tcgacctgca
     3121 tcgccggttc cggttccttc gccggccgca acaggttggg cgccacccac aggaagcgaa
     3181 ccggcgtcaa tgccaccacc cggtacatgc ttggacgcag ccgtgcgatc ggcaatgcgg
     3241 cgctttcgct gccgctcttg atgaccgacc tgcgaccatc gcgcgcctcc agcgccagag
     3301 tgccttcgac gagaaacagc tcccctcgtt cggtgctgcc aagattcagc aggaccgttt
     3361 tggcgggcgc cgagcgcagt gaaagctgat cgctcagcgc actcaaccat gcatcgtcgc
     3421 aactgctgaa ggacgagaac aatttcaact gttcgggcaa gacccgttcc atcgttgcag
     3481 tgctcataac caccgattca ctttataacc tgatcaaatc acaaaccgtg ccatcaatgg
     3541 ccgtcgccct tcagaacttc gccgccgact ccagcagcaa aaccagatca tcgtcggcaa
     3601 tatcgatgag ctgaaaacca gtgtagtact ggctgccttc ggcattctga ctccagagcg
     3661 catcagcccc cagatggatg tgggtttttc ccgccacagg cgttgtcaac tccagacgca
     3721 gccgatagag ccgatcggtc acgaccggcc ggtcacccgc caccatcacc cccccttgcg
     3781 agacattgac cacgacaccc attctgctgc catcgccgcg gtcgatcacc tggagttctt
     3841 gatccagggt gattcttgga gattttctca actcatccat tcagattccc cacgaaaaga
     3901 ccgctctgtc caatctgcaa acatggagca agccgctcag actgcaccgg ccctccggcg
     3961 caaccgcctg gagagcaggt catgaatggt gctcatcgca cggtcgatca gtggctcctc
     4021 ggcttggcgg atcaccttgg catcgccact caacagactg ttggccagcg aaatcagcga
     4081 cctcactccg acctgcttgc cgttttgatc gacaaacatg taacgcgatg tcagaggact
     4141 gaaccaagag agtttgccgc gctttttgtc accctgggca ttgatggtga attcaaacca
     4201 ggtgccgaag ggcactttgc gcagctcggc aatggcttgc tgcagctcag gtgaaagatt
     4261 gcccgtcgaa ctggcctctg ccgcccgcac tctgttgcgc tgaccaggcg gtttttcagc
     4321 aactggctgc tgtactgcga cctgctcggc gcgcgcctcc aacgcacggc tctgcgccgc
     4381 ggccagttca cgcagcagct gtttcgcttc gccctgatac ccccccagca gcacaaaccc
     4441 ctgctgaatg gcttcgttca actcgggaag ctgccgacgc aacgcactct tttcctcctc
     4501 gctcaccttg ggcgtgacgc tccagatgac atcatcgacc accttcagcg tgttgtgcca
     4561 gcttgcactc tccgttccct tgcgcagcag cgtgaagacc agaatgtcga cccacggctg
     4621 ctcaagaaaa cgggtgaccg cctcgggcag atcatggccc tcggtgcggc tcatcacctc
     4681 ctgcaccgcg cggttgcggg ccgtgcgcaa cttctcctgc ccctgcaatg tctccatcgc
     4741 ccgcttttcg gccaggtcgg cgcgcttttc cagcttttcg atgaactgat tgaaatcagc
     4801 caggacctcg tcgaacagtt cgatctccag cgtatattcg agcaggatgc ggtccacgat
     4861 cgaacgcatc ttggcaaaca cgccatgctc ctgatcatca tcgatcaacc aatgtccacc
     4921 cgccttggcc attgcattca acagccgccg agccggatgg ctggagcggg tgaaaaactg
     4981 tcgatcgagc agcgccacct tcaggaaggg ggtgtgcaga tggctcaaca gcgccttcac
     5041 cgaatcgggc agattctcgt cgttgagcat gtactcgaac agcatgccga ccaggtcgat
     5101 caggtcggca tcgaccgtag cgacctgcct cacctctccg gcaatgctgt tgagcttctc
     5161 gacctgctcc agcagctggc gcttgactgc ttcgaccgtc agcagttgcg catggtcgac
     5221 ctgagcggca gattgatact ggtgatgctg aatctgatcg accgtctgcg ccaattggtg
     5281 aggcgtgtaa ctggcaccat cgcccaccag tgccacgcca tccgggcgca tcgaccccat
     5341 gctgcgcagc gatggcgccg acatcaacgg cggcgctcct gccatggccg ctgattgccc
     5401 cccgccaggc accgcagcca tcgcggaagg cacgaacggc atgaactgca ctgcgggcat
     5461 cggcaccccg cctgtcgaat aggccagcgc tggatccatt gccgggtgcg caacagcccc
     5521 cgtcgccatg ggctcctgca ccaagggcag aggctgcgcc agcatcgact gaacctgggc
     5581 aaacagctcc gacggcgatg tgggatgggc atcgaaaccg gatggctgcg gcacccgctc
     5641 ctgaagcgaa tcggaaggtt cgatcttttc ttcggttgtc ggccgctgcg aactgccgct
     5701 cgctccatgc aggcggcgga cttcaaaagc cagattcggc agaatgtccg cctcgatcag
     5761 caggtcgttg cagcggtcat acagccggcc cagcctctcg agcacctcgg tgccgaagga
     5821 tttcaaaacc agttgcctga tctcttcgga aagattgagc aagcggacag caccacggca
     5881 agcctctccg agccgctccg ggccaaaggg gttgtcacac tcttcgagct ttcggccctg
     5941 cgacaggatt gccaggcgct gattcagggc atggatcgac tgatggaact gcgcattggc
     6001 gctttgaacc atgttgccaa tgatgatctc ttcattgagc acatgttcat ccaccagtga
     6061 caggccaccg ccggtcgcag ccggtcgccc accgatcggc ggcagtggct gattgcgcgc
     6121 aaaccggttg atctgttcgc tcaccccctc cagaaagtgc tccgttacca tcggccagcg
     6181 gaaacgaatc tctcgcaagt catcaaaaac ggcggcctgc tcctggttgc tgcgcgcctg
     6241 ttcggctttt tcaaagacct tctcttcgat ctgttcaaag gtgcgctgca ccgctgaggt
     6301 gacctggctg atcacctcac tgcgcaattg atcgatgagt tgacgctggc tgaagtgcat
     6361 gaccaatcgc cccaaaacct ctcgtctcgc tgcaggccta tttacagtgg ccaactataa
     6421 catccttgcg gttccagcaa ggccgaagac tcgacccgaa tgccaaccgt cggcggcttt
     6481 tttcgccgat cgcaattttg ctaccatgcg gcgcctcgac gacccgctca ggaatcccgg
     6541 aacagcaatg cctccctctt cctatccccc ggcagggctg ctgcgccgct tgggcgcgat
     6601 gctctacgac acgctgctgg tcgttgcgct gctgatgtgt atcactgctg cctacatctt
     6661 cattgcgctg caactgcagg gcgatacgct ggccgccggc aaggtggcgg cgcggggggc
     6721 gctctatcag agcattctgc tgatttcgac cttcggcttt tttgcctatt tctggacccg
     6781 acatggacag accctcggca tgcaggcctg gcgtctgcgc attcagaaca gcgatggctc
     6841 gaccatcagg ctgccccagg cggtactgcg cttctttggg gcactgtttt cgctgctctg
     6901 tctcggattg ggctatggct ggatgctggt cgaccgcgac cggatgacct ggcatgaccg
     6961 gctgtcatct tcgctggtgg tccaactgcc caagccggag aagaagagga aaaagcgcgg
     7021 cggatagcgt tcagccagcg cgcctcaacc aacgcaacgc aaacagcaga cagagcaatg
     7081 gtggcagtgc cgccgcccag atcggcgaaa actgaaacac gatgctggcc ggccccagga
     7141 tgttctgggc gaaatgaaac accacgccta ccgcaatccc ggcaaagacc cgcgtctcca
     7201 tcgtcaattc gcgcagcggc ccgaaaacga acgaaattcc aatcagcacc agcgccaggg
     7261 ttgccgccgg ctgaaacagc cgcttccaga atgccagcca gtgcggcgcc gactccagcc
     7321 gctgctgatc gaggtgaacg gcatagcggt agagctcgat ggccgaaagt cgatccggtt
     7381 tcagtgccag cacctgcagc agcgccggag tgagggtgct tggccaagcc tgttgtgcca
     7441 gctgctcgct ctgactctgc tccgccgtca gccaggtggc ggccacctgg tcgagctgcc
     7501 atcgcccctc ggcgtaggag gcccgttgcg catgactgag acgaaccaac tggcgctcct
     7561 gatcgaactg gtaacgggtc acacccagca ggtcgccctg cgcggtgatg gcactgaagt
     7621 agacgaaatc aggaccatca cgcaaccagg tcccctcacc cgcacgccca tggccgtcgc
     7681 cattgagctg cgctcgccag ttttgtgcgg cctgttcggc agccggggcc acccactctc
     7741 ccagcagggt cgtcgccacc atgaacagca ataccggacg gcaggccgac caggcgattc
     7801 cc
//
LOCUS       DAORCQ010000105         7702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6714_length_7702_cov_5.022362, whole genome shotgun sequence.
ACCESSION   DAORCQ010000105 DAORCQ010000000
VERSION     DAORCQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6714_length_7702_cov_5.022362"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKI23_09830"
     CDS             complement(<1..441)
                     /locus_tag="PKI23_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNN37274.1"
                     /translation="MLLAIREFFERMCQPAADQGDASHALRLATAALLVEVMRADHAF
                     DPREREALLTLLQQHQQLAPEEVETLLELAEQAQREATSLFQFTDLIRDHYSYPQRVA
                     LIEAMWRIALADNRLDRYEEHLIRKVAELLYVSHRDFIGAKLHVQ"
     gene            complement(464..1435)
                     /gene="dusA"
                     /locus_tag="PKI23_09835"
     CDS             complement(464..1435)
                     /gene="dusA"
                     /locus_tag="PKI23_09835"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNN37275.1"
                     /translation="MRLDRRLSIAPMMGCTDRHARFFLRLFSPRLLLYTEMITSAALC
                     HGDPARFLDHDAAEHPLALQLGGSDPRELAAAARIGEQWGHDEINLNLGCPSDRVQAG
                     RFGACLMKEPTLVEECLRAMAEAVQVPVTAKIRIGVDEADSFELLCRFVETIARTGVG
                     SITVHARKAWLKGLSPKENREIPPLDYPRVHRLKQHFPQLEIILNGGLKTLDQVSEQL
                     AFVDGCMIGREAYHNPAILLEAEQRIYQQAGEPASRLEILQRLHPYIEQQLTLGVPLH
                     AMTRHLLGLFQGQPGARHFRRHLSEQGPRRRHDVRLLHEAVALLHAA"
     gene            1682..2161
                     /locus_tag="PKI23_09840"
     CDS             1682..2161
                     /locus_tag="PKI23_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNN37276.1"
                     /translation="MTLKAGDSIPAATLATMTEAGPKPLTTDEIFKGKKVVLFAVPGA
                     FTPLCSGQHLPGYVKLAKEIKAKGVDTIACVSVNDVFVMGAWGKSQGADEILLLADGN
                     AAFTKAMGLELDGTPMNLGIRSQRYAMIVDDGVIKTLQVEEGRNFAVSNAESMLALL"
     gene            2211..2969
                     /locus_tag="PKI23_09845"
     CDS             2211..2969
                     /locus_tag="PKI23_09845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNN37277.1"
                     /translation="MFPLYDENPSVRLPLTTIALIIGNGLVWLLLQQLGSSEGLARSI
                     CQHGLIAGELLQRVPPGSSFALDAQFRCVLDGSGGWLTLFSSMFMHGGWMHLIGNMWF
                     LWVFGDNVEDAMGHSRFLLFYLLCGLAAAAAQIATNPASTVPMVGASGAIGGVMGAYA
                     RLYPRARIQMLVILGFYVTRVAVPALFMLGYWLLLQLLGALPALGRLGGGVAFWAHIG
                     GFAAGVVLAGPLTRADYLQQHLRQPPRRGARYRF"
     gene            2989..4278
                     /locus_tag="PKI23_09850"
     CDS             2989..4278
                     /locus_tag="PKI23_09850"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNN37278.1"
                     /translation="MSLLHATSRWLRSPVVAWALYDWANSAFALVVLTTFFPLFFKTH
                     WNAGVPAELSTFRLGLTNSLTIALVVVSAPLLGALADTGSGKKPLLTLFLLIGLIGTL
                     GLWWFDSGPWWVAALLFGLAYLGFATSNVFYDALLIDVSPPDRLEWNSALGFSLGYIG
                     SSLLFMFDLLLIQDPQRFGLDSQSEAIRFGFLSVALWWGLFSLPLLWRVHERPLPARA
                     AGPRIRQSLQALRATVRLLFRERTIGLFLLAYWLYIDGVDTVIAMAVDYGLAIGLEAS
                     DLLQAILLTQLVGFPAALLFGWLATRSGIRPALYIGLAVYMLVTLGGAWLNSAWQFYL
                     LAVTIGLVQGGVQSLSRAYFARVIPAERSGELFGFYNMIGKFAAVLGPLLVGLTSWLS
                     GSNRLGLLSLLLLFGSGLWLLTRLPVLPPPSGTLSND"
     gene            complement(4300..5886)
                     /locus_tag="PKI23_09855"
     CDS             complement(4300..5886)
                     /locus_tag="PKI23_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNN37279.1"
                     /translation="MSNSAVLQEISRRRTFAIISHPDAGKTTITEKLLLFGNAIQMAG
                     TVKGKKSGRHATSDWMEMEKERGISVTTSVMQFPYHERVINLLDTPGHADFSEDTYRT
                     LTAVDSALMVIDGAKGVEARTIKLLEVCRLRDTPIFSFINKLDRDIREPIELLDEIEQ
                     VLGIECAPITWPIGMGRHFRGVYHLERDLIYLYTPGMGHTIADERLIVGIDSPQAQAE
                     LADEMAELKEQIELVRGASHPFDRERFLAGKLTPVYFGTALGNFGVREMLNDFIEQAP
                     PPRPRAARERLVEVAEPNFSGFVFKIQANMDPRHRDRIAFMRICSGRYQPGMKLYHSR
                     LGKEVRVADALTFMAGERSQTEEAYAGDIIGLHNHGTIQIGDTFTTGEPLQFTGIPHF
                     APELFMRVRLRDPLRLKQLQKGLTQLSEEGATQLFMPLNSNDLILGAVGSLQFDVAAY
                     RLREEYGVDCIHEPVNIHSARWISCADERHLDEFRRKAADQLAIDGGGHLTYLAPSRV
                     NLELMRERWPKIEFRATREH"
     gene            6004..6582
                     /locus_tag="PKI23_09860"
     CDS             6004..6582
                     /locus_tag="PKI23_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNN37280.1"
                     /translation="MNNDEFARLVREHHRALIGIATPIVGPSEAEEVVQNAWLKAHRS
                     IGQFEGRAQIRTWLGRIVINEARMQRRKRRRESLFSDWLPAEDETEGDIMAERFSPNG
                     HWNTPPMQWNLDTPEALLLREDLAECLQKLLDTLPASQRAVLELRDTLELSFEEICNE
                     LSISASNAKVLLHRARLKLFRLVDHYEETGEC"
     gene            6576..6815
                     /locus_tag="PKI23_09865"
     CDS             6576..6815
                     /locus_tag="PKI23_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNN37281.1"
                     /translation="MLNSCRKVVESADQLLDGTLPWRQRLSLRLHLLLCNACRRYLRQ
                     LRALLRAIPFMHGEASDEEVQRVMAHIQTHPDQHS"
     gene            7191..7313
                     /locus_tag="PKI23_09870"
                     /pseudo
     CDS             7191..7313
                     /locus_tag="PKI23_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252796.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7379..7621
                     /locus_tag="PKI23_09875"
     CDS             7379..7621
                     /locus_tag="PKI23_09875"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNN37282.1"
                     /translation="MIGSASKQPLAGAGLGLRRALLGALENESSRQPDFLEVVPENWL
                     RIGGQLRHRFDALAQQRPLVCHGLSLSLGGPDPLDW"
BASE COUNT         1327 a         2414 c         2468 g         1493 t
ORIGIN      
        1 ctgcacatgc agtttggcgc cgatgaagtc gcggtgtgag acgtagagca attcggccac
       61 cttgcggatc agatgctcct cgtagcggtc gaggcggttg tcggccagcg cgatgcgcca
      121 catcgcctcg atcagtgcca cccgctgggg gtagctgtag tgatcacgaa tcaggtcggt
      181 gaactggaac agcgaggtgg cctcgcgctg cgcctgctcc gccagttcca gcaaggtctc
      241 gacctcctcc ggtgcaagct gctgatgttg ctgcagcagc gtcagcagcg cttcgcgctc
      301 acgcggatcg aaggcatggt cggcacgcat cacctcgacc agcagtgccg cggtggccag
      361 ccgcagcgca tggctggcgt caccctggtc cgcagcgggc tgacacatcc gctcaaagaa
      421 ctctctgatc gcaagcagca tggtggtgct ccggtgggtc gattcaggcg gcgtgcagca
      481 gcgctaccgc ctcatgcagc agccggacgt cgtgccgtcg ccgtggcccc tgttcgctca
      541 gatggcggcg gaagtggcgc gcgccgggtt ggccctgaaa caggcccagc agatggcggg
      601 tcatcgcatg cagcggcacc cccagcgtca actgctgttc gatgtaggga tgcagccgct
      661 gcagaatctc cagacgactg gccggctcgc cggcctgttg gtagatccgc tgctcggcct
      721 ccagcaggat ggccggattg tggtaggcct cgcgaccgat catgcagcca tcgacgaagg
      781 cgagctgctc gctcacctga tcgagcgttt tcaaccctcc attcaggatg atctcgagct
      841 ggggaaagtg ctgcttgagc cgatggaccc gcgggtagtc gagtggcgga atctcgcggt
      901 tctcctttgg actcagcccc ttcagccagg ccttgcgggc atggacggtg atgctgccga
      961 ccccggtgcg ggcgatggtc tcgacaaagc ggcacagcag ctcgaaggag tcagcctcat
     1021 cgacgccgat gcggatcttg gcggtgaccg gaacctgcac cgcttcggcc atcgcccgca
     1081 ggcactcctc gaccagtgtc ggctccttca tcagacaggc gccgaaccgg cccgcctgca
     1141 cccggtcgct ggggcagccg aggttgaggt tgatctcatc atggccccac tgctcgccga
     1201 tgcgcgctgc tgctgccagc tcccgcggat cactgccacc cagctgcaac gccagcggat
     1261 gttcggcggc atcatggtcg agaaaccggg cggggtcgcc atggcagagc gccgcactgg
     1321 tgatcatctc ggtgtagagc agcagccgtg gcgagaacag ccgcagaaag aagcgggcat
     1381 ggcggtcggt gcaacccatc atcggcgcga tgctgaggcg acgatcgagc ctcacgggca
     1441 gaacagcagt ggtcaagggc aatttccggg ttgaacagag ggcatgatta tagctgcatg
     1501 acatcgccac ggtggtgaaa caggtcggct ccgcactggt ttcttgaaat caaccatttg
     1561 ttttgaatgg tattttggca gcgacagagg tgacaagggc aagtcgtgca tggtattgat
     1621 agccacggcg cgctctgcgt gcagtcagga ttttcgttca ttcacctcag gagcaactcc
     1681 aatgacactc aaagcgggcg attccattcc tgcggccacc ctcgccacca tgaccgaggc
     1741 cggccccaag ccactcacca ccgatgagat cttcaagggc aagaaggtgg tgctgttcgc
     1801 ggtgccgggc gccttcaccc cgctctgcag cggtcagcac ctgcccggct atgtcaaact
     1861 cgccaaggag atcaaggcca agggggtcga caccattgcc tgcgtctcgg tcaacgatgt
     1921 cttcgtgatg ggtgcctggg gcaagtcaca gggtgccgac gagattctgc tgctggccga
     1981 tggcaatgct gctttcacca aggcaatggg gctggagctc gacggcacac cgatgaacct
     2041 cggcatccgc tcgcagcgct atgcgatgat cgtcgacgat ggcgtgatca agacgttgca
     2101 ggtcgaggag gggcgcaact tcgccgtcag caacgcagag tcgatgctgg cactgctgta
     2161 acccggcccc cgatggcggg ccctgcccgc catcagcctg acccacgcgc atgttcccgc
     2221 tctacgatga aaacccttcg gtccggctgc cgctgaccac gattgcactg atcatcggca
     2281 atggcctggt ctggctgctg ttgcagcaac tcggcagcag cgaggggctg gcgcgctcga
     2341 tctgccagca tggcctgatt gcgggtgagc tgctgcaacg ggtgccgccc ggtagcagct
     2401 ttgcgctcga tgcgcagttc cgctgcgtgc tcgatggttc cggcggctgg ctcacgctgt
     2461 tcagttcgat gttcatgcat ggcggctgga tgcacctgat cggcaacatg tggtttctgt
     2521 gggtttttgg cgacaatgtc gaagatgcga tgggccacag ccgctttctg ctcttctatc
     2581 tgctgtgcgg tctggccgct gctgcggcgc agatcgccac caatccggcc agcaccgtgc
     2641 cgatggtggg tgcatcgggc gcgattggcg gcgtcatggg cgcctatgcc cggctctatc
     2701 ccagagcgcg catccagatg ctggtgattc tcggcttcta tgtcacccgg gtcgcggtgc
     2761 cggcactctt catgctgggc tactggctgc tgctgcaact gctgggcgcc ctgccggcac
     2821 tcggccgcct ggggggcggg gtggccttct gggcccacat tggcggcttc gcggccggcg
     2881 tggtgctggc cgggccgctg acgcgcgctg actacctgca acagcatctg cgccaaccgc
     2941 cacgccgcgg cgcgcgctac cgcttttagc cgccacggaa acccgctcat gtccctgttg
     3001 cacgccacat cgcgctggct gcgctcaccc gtggtggcct gggcactcta tgactgggcc
     3061 aactcggcct tcgcgctggt ggtgctgacc accttctttc cgctcttctt caagacccac
     3121 tggaatgccg gtgtgccggc cgaactgagc acctttcgcc tcggcctcac caacagcctg
     3181 accatcgcgc tggtggtggt cagcgcgcca ctgctgggtg cactggccga caccggcagc
     3241 ggcaagaagc cgctgctgac gctctttctg ctgatcggtc tgattggcac gctggggttg
     3301 tggtggttcg acagcggtcc ttggtgggtg gccgcactgc tgttcggact ggcttacctc
     3361 ggctttgcca ccagcaatgt cttctatgac gcgctgctga tcgatgtgtc gccccccgat
     3421 cggctggagt ggaactcggc gcttggtttc agcctcggct acatcggcag cagcctgctg
     3481 ttcatgttcg acctgctgct gatccaggat ccgcagcgct tcgggctcga cagccagtcc
     3541 gaggcgattc gcttcggctt tctgtcggtg gcgctgtggt ggggcctctt ctcgcttccg
     3601 ctgctgtggc gcgtgcatga gcgcccgctg ccagcccgtg ccgccggccc gcgcatccgc
     3661 cagagcctgc aagcgctgcg tgcgaccgtg cggctgctgt tcagggaacg caccatcggc
     3721 ctgtttctgc tggcctactg gctctacatc gatggcgtcg acaccgtcat cgccatggcg
     3781 gtcgactatg gcctggcgat cgggctcgag gccagcgacc tgctgcaggc gattctgctg
     3841 acccaactgg tcggttttcc tgcggcactg ctgttcggct ggctggccac gcgcagtggc
     3901 atccggcccg cgctctacat cgggctcgcg gtctacatgc tggtgacgct gggtggcgcc
     3961 tggctcaaca gtgcatggca gttctacctg ctggcggtca ccatcggtct ggtgcagggc
     4021 ggtgtgcagt cactgagccg ggcctacttc gccagagtga ttccagccga gcgttccggt
     4081 gagctgtttg gcttctacaa catgatcggc aagttcgcgg cggtgctggg accactgctg
     4141 gtcggactga cgagctggct gagcggcagc aaccggctgg ggctgctgtc actgctgctg
     4201 ctgttcggca gcggcctctg gctgctgacg cggttgccgg tattgccgcc cccctccggc
     4261 accctctcga acgattagca gccgcgccag cgggcggtct caatgctcgc gggtggcgcg
     4321 gaactcgatc ttgggccagc gctcgcgcat cagctccagg ttgacccggc tcggcgccag
     4381 ataggtgaga tggccgccgc catcgatggc cagctgatcg gccgccttgc ggcggaactc
     4441 atcgaggtga cgctcgtcgg cgcaactgat ccagcgcgcg ctgtggatgt tgaccggttc
     4501 gtggatgcag tcgacgccat actcctcgcg cagccgatag gccgccacat cgaattgcag
     4561 gctgccgacc gcacccagga tcagatcatt gctgttgagc ggcatgaaga gctgggtggc
     4621 gccctcctcc gacagctggg tcaacccctt ctgcaactgc ttcagtcgca gcggatcgcg
     4681 cagccgcacc cgcatgaaca gctccggagc gaagtggggg atgccagtga actgcagcgg
     4741 ctcgccggtg gtgaaggtgt caccgatctg gatggtgcca tggttgtgca gaccgatgat
     4801 gtcgccggca taggcctctt cggtctggct gcgctcgccg gccatgaagg tgagcgcatc
     4861 ggccacccgc acctccttgc ccagacggct gtggtagagc ttcatccccg gctgatagcg
     4921 gccggaacag atgcgcatga aggcgatgcg gtcgcggtga cgcggatcca tgttcgcctg
     4981 aatcttgaag acgaaaccgc tgaaattggg ctccgccacc tcgaccagcc gctccctggc
     5041 cgcacggggt cgcggcggcg gcgcctgctc gatgaagtca ttgagcatct cgcgcacacc
     5101 gaaattgccc agcgcggtgc caaagtagac cggcgtcagc ttgccggcca gaaagcgctc
     5161 gcggtcgaac gggtggctgg cgccgcgcac cagttcgatc tgctccttca gctcggccat
     5221 ctcgtcggcc agctcggcct gcgcctgcgg tgagtcgatg ccgacgatca gccgctcatc
     5281 ggcaatcgtg tggcccatgc cgggcgtgta gaggtagatc agatcgcgtt cgaggtggta
     5341 gaccccgcga aaatggcggc ccatgccgat cggccaggtg atcggggcac attcgatgcc
     5401 gagcacctgt tcgatctcat ccagcagttc gatcggctca cggatgtcac ggtcgagctt
     5461 gttgatgaac gagaagatcg gcgtgtcgcg cagccggcac acctcgagca gcttgatggt
     5521 gcgtgcctcc acccccttgg cgccatcgat caccatcagc gccgaatcga ccgccgtcag
     5581 ggtgcgatag gtgtcctcgg agaagtcggc gtggcccggg gtgtcgagca ggttgatcac
     5641 ccgctcatgg taggggaact gcatcaccga ggtggtgacc gagatgccgc gctccttctc
     5701 catctccatc cagtcggagg tggcatggcg ccccgacttc ttgcccttga cggtgccggc
     5761 catctggatg gcattgccga acagcagcag tttttcggtg atggtggtct tgccggcatc
     5821 cgggtgcgag atgatggcga aggtgcgtcg acgcgagatt tcctgcaaca ctgcgctatt
     5881 ggacatcagg tcggggtttc acttttgcag atcggaccga tcgggggcgg cattctcgct
     5941 gatcacaggg ttgcggacaa ttcatgcttc aatgcaggtc aaatgacatg gaaatggcca
     6001 gtgatgaaca atgacgaatt tgcccggctc gtgcgtgagc accaccgtgc actgatcggc
     6061 atcgccaccc ccatcgtggg cccctccgag gccgaggagg tggtgcagaa tgcctggctc
     6121 aaggcccatc gatcgattgg tcagttcgag gggcgtgcgc agattcgcac ctggctcggt
     6181 cgcatcgtca tcaatgaagc cagaatgcag cgccgaaaac gcaggcggga gtcgctcttc
     6241 agcgactggc tgcccgcgga ggatgagaca gaaggcgaca tcatggcaga gcggttcagc
     6301 cccaatggcc attggaacac cccgccgatg cagtggaacc tcgacacccc cgaagcactg
     6361 ctgctgcgtg aggatctggc ggagtgtctg cagaagctgc tcgacaccct gccagccagc
     6421 cagcgtgccg tgctggaact gcgtgacacc ctggaactct ctttcgaaga gatctgtaac
     6481 gagctgtcga tttccgcatc caatgccaag gtgctgctgc atcgggcaag actcaaattg
     6541 ttcagactgg tggatcacta cgaggagacc ggcgaatgct gaactcttgt cgcaaggtcg
     6601 ttgagagcgc cgaccaactg ctcgatggaa ctctcccctg gcgtcagcgg ctgtcgctgc
     6661 ggctccacct gctcctctgc aacgcctgtc gtcgctacct gcgccaactg cgtgccctgc
     6721 tgcgcgccat cccgttcatg catggcgaag ccagtgacga agaagtgcag cgcgtcatgg
     6781 cgcacattca gacccatccg gatcagcact cctgagcgca ccctctgcca ctgcgcagtg
     6841 gcaccctttg gtcaaccaga caatttctcg cctcccctgt aaccaatcgg caggccgtgc
     6901 atccaaccgg tacattgcag ccctgatggc cgacggatca tctgccgtgc ccaagcctgc
     6961 aatgcaacga tcggcacagc gggagaccgc ggcaggctga cccaaacgct ctgccgcgat
     7021 cactgtctgc tgaaccgaac ccgatgacga tcatcatgaa tatggagaga aacatgcaga
     7081 gcaagtcgat caacagcctc aaaggtgccc ttggcgtggc ctttctgtcc ggctccctcg
     7141 ccgggcccgt gcttgccgac acgaccggaa atcctttcgt cgccaatgat ctcggcagcg
     7201 gctatcaggt cgcagagaaa ggtggcgaag gcaaatgcgg tgaaggtaaa tgcggtgagg
     7261 gaaaatccgc taaaaaggag ggggaaggca agtgcggtga agccaagtgt ggcgcagaca
     7321 aggccaagga gaaatccgcc gaagccgaga agcactgaca gcccgcgagg tgaagaccat
     7381 gatcggctct gcttcgaaac agcctctggc tggcgcgggt ctcggtctgc ggcgtgccct
     7441 gctgggcgcg cttgagaacg agagcagtcg acagcccgac tttctcgaag tggttcccga
     7501 aaactggttg cggatcggtg gccagttgcg ccaccgattc gatgccctgg cacagcagag
     7561 acccctggtg tgtcatggcc tctccctgtc gctggggggg cctgatccgc tggactggta
     7621 atttctcgca gagattcgtg actttctgca acagcaccgg gtgttgcact acacggagca
     7681 tctgagctat tgcagcgatg gc
//
LOCUS       DAORCQ010000106         7568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6879_length_7568_cov_6.704113, whole genome shotgun sequence.
ACCESSION   DAORCQ010000106 DAORCQ010000000
VERSION     DAORCQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6879_length_7568_cov_6.704113"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKI23_09880"
     CDS             complement(<1..278)
                     /locus_tag="PKI23_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNN37283.1"
                     /translation="MSLTVEQTKARNKELIDEVLKVYPDKTVKKRAKHLNTFEEGKTD
                     CGVKSNIKSVPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYS"
     gene            complement(424..1311)
                     /gene="nifH"
                     /locus_tag="PKI23_09885"
     CDS             complement(424..1311)
                     /gene="nifH"
                     /locus_tag="PKI23_09885"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039800829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HNN37284.1"
                     /translation="MAMAELRQIAFYGKGGIGKSTTSQNTLAALADMGQKILIVGCDP
                     KADSTRLILHAKAQDTILSLAAEAGSVEDLELEQVMKTGYKDIRCVESGGPEPGVGCA
                     GRGVITSINFLEEEGAYEGIDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMA
                     MYAANNISKGILKYANSGGVRLGGLVCNERQTDKELELSEALAAKLGTKLIHFVPRDN
                     VVQHAELRRMTVMEYAPESKQAEEYRTLARKIHENGGKGTIPTPITMDELEDLLMEHG
                     IMKRVDESIVGQSAASAVA"
     gene            1765..2193
                     /locus_tag="PKI23_09890"
     CDS             1765..2193
                     /locus_tag="PKI23_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37285.1"
                     /translation="MQVGVGSATAVENKRVFIVDSDEIIRAVLQFMLHDEYEAHELAT
                     PAAAVAKGIDWKPDLLVLGGATLRELGLAGLPQIKSELGGVKILLVTEAGENVVAQAA
                     LDAGVADALLLQPLTVESVRRTVNRLCGRRTQRTVKLHQI"
     gene            complement(2245..3240)
                     /locus_tag="PKI23_09895"
     CDS             complement(2245..3240)
                     /locus_tag="PKI23_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HNN37286.1"
                     /translation="MNQFRALQTSEIRDGLYGSSVVTRSIDQLPEGELLIAVHYSSLN
                     YKDALSASGHRGITRHYPHTPGVDAAGVVQASTHPDFTPGMPVVVTGYDLGMNTAGGF
                     AEYIRVPAAWAIPLPTELSPREAMILGTAGLTAAIAVDKLQRMGLLPEQGEVLVTGAT
                     GGVGSLAVALLARLGYEVVASSGKIEQADYLSKLGAKRVIDRDTLAERSPRALLKPLW
                     QGAIDTVGGDTLEQILKSLNYGGSVACCGQLSSTSLSSSIMPFILRGVNLLGVDSVEL
                     APAIKAAFWRRLASSWDSLPLDLIAREITLEELPTQIERMLAGKSVGRVLVRVGA"
     gene            complement(3237..4307)
                     /gene="sohB"
                     /locus_tag="PKI23_09900"
     CDS             complement(3237..4307)
                     /gene="sohB"
                     /locus_tag="PKI23_09900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HNN37287.1"
                     /translation="MTAALLEYALFLAKGLTLLILVLLLVAGIAALSQRQRKAAHGQI
                     EVRKLNHEWEQQADILRHATLSEPALKLWKKERKQLEKARKKGDVLPKKHIYVIDFKG
                     DIRASQTRQLREEISAILAVATAADEVLVRLESSGGMVHAYGFAASQLDRIRNHGIAL
                     TICVDMVAASGGYMMACIANRLLAAPFAMIGSIGVVAQLPNFHRLLKKHEIDYELLTA
                     GEYKRTLTLFGENSEKGREKFIEELEETHALFKHFVTSHRPQLAIEQVATGEVWYGQK
                     ALTHGLIDELMTSDEYLGRQVNEADIFKVAYVEKRSLPERMGLSMGQLRQRLGGHLSR
                     PAASMAMAENLSLQQQIAGELP"
     gene            complement(4370..5182)
                     /gene="nudC"
                     /locus_tag="PKI23_09905"
     CDS             complement(4370..5182)
                     /gene="nudC"
                     /locus_tag="PKI23_09905"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNN37288.1"
                     /translation="MSEFEPGNLRPHGYQESYCIAFVGDRLVMSTEAPHWQPLDQGQC
                     RWLNLDRDSEHYIGTFRGHPCFTAEATEPLPSGFVLTDLRALLSLANGDLFAAASRAW
                     QMVQWHRTHRYCGRCASPMQDHATDRAKECPNCRLLNYPRLSPCIIVLVTRGEELLLG
                     RSPQFPPGMFSTLAGFIEPGESAEQALYREVLEEVGVQVTNPRYLSSQAWPFPNNLML
                     GFHADYLAGEVKADGVEVVEAGWFHYKNLPKVPGSVSISGWLIESYLRQLQQ"
     gene            complement(5334..5921)
                     /locus_tag="PKI23_09910"
     CDS             complement(5334..5921)
                     /locus_tag="PKI23_09910"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNN37289.1"
                     /translation="MAGKLTRGDLLSLEQYAEQRPLFRARIIEHKRNRRLPIGPCAVL
                     YFEDRLSMQYQIQEMLRAERIFESAAIQDELDAYNPLIPDGTNLKATFMIEIEDAVER
                     RAALAQLVDIENRVWIRVAGFDAVHAIADEDMERADSEKTSSVHFLRFEFTPAMIAAI
                     HNGAPLAAGIDHPGYQHWVDPIPRHIRDSLGNDFS"
     gene            complement(5989..7233)
                     /locus_tag="PKI23_09915"
     CDS             complement(5989..7233)
                     /locus_tag="PKI23_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNN37290.1"
                     /translation="MSFATLGLANEILRALSDQGYTDPTPIQQQAIPTVLAGGDLLAA
                     AQTGTGKTAGFTLPILQRLADTPAPANGHRPIRALILAPTRELAAQIEESIRGYGKYL
                     RSTSTAIFGGVGINPQIERLRRGVDILVATPGRLLDHLQQRTVNLSQVEILVLDEADR
                     MLDMGFIHDIRRILALLPKKRQNLLFSATFSNEIKTLANTLLNSPTHIEVARRNAPAD
                     AVTQKVYRVDRERKRELLTHLIRQHQWYQVLVFTRTKHVANRLAEQLDKSGIPALAIH
                     GNKSQNARTRALSEFKLGQLQVLVATDIAARGIDIDELPHVINFELPNVAEDYIHRIG
                     RTGRAGAQGEAISLVCVDEAKLLTGIERLLKRTLPSETVPGFEPDPSIKPQPELIMQR
                     GNRMKPTPRQANGNVRSGQRHG"
BASE COUNT         1345 a         2204 c         2456 g         1563 t
ORIGIN      
        1 gagtactggc cgcagccgac cgggccgtgg gagatgtgga tcatgtcctt gatcggcccc
       61 cacaccacac ccttggagcc ggcataggca caaccacgaa tggtcatgac gccggggacc
      121 gacttgatgt tcgacttgac gccgcagtcg gttttaccct cttcgaaggt gttgaggtgt
      181 ttggcgcgct tcttgacggt cttgtccgga tagaccttga gcacctcatc gatcagctct
      241 ttgttgcgtg ccttggtttg ctcgacggtc aggctcatgt tggcctcctc attgacgttg
      301 aactgaaatt ctgtgaaccc cggcggatgg cgccgtcctg ctggttgact gcgctgcttc
      361 tggcgccatt cgcggagatt ccacccctgt caccaggaga ggggtggatt ccgttggcgc
      421 gcctcaggcg actgccgacg cagcgctctg accgacgatc gactcatcga cccgcttcat
      481 gatgccgtgc tccatcagca gatcttcgag ctcatccatg gtgatcgggg tggggatggt
      541 gcccttgccg ccgttctcat ggatctttct ggccagcgtg cggtactcct cggcctgctt
      601 gctctccggt gcgtactcca tcaccgtcat ccgccgcagt tcggcgtgct ggacgacgtt
      661 gtcacgcggc acgaagtgga tcagcttggt gccgagcttg gcggccagcg cttcggagag
      721 ttccagctcc ttgtcggtct ggcgctcgtt gcacaccagg ccccccaggc gcacgccgcc
      781 ggagttggcg tacttcagaa tgcccttgga gatgttgttg gccgcgtaca tggccatcat
      841 ctcgccggac atgacgatgt agatctcctg cgccttgttc tcgcggatcg gcatggcaaa
      901 gccgccgcac accacgtcac cgagcacgtc gtaggagacg tagtcgatgc cttcgtaggc
      961 gccttcctct tccaggaagt tgatcgaggt gatcacgcca cgcccggcgc agccgacacc
     1021 cggttccggt ccgcccgact cgacgcagcg gatatccttg tagcccgtct tcatcacctg
     1081 ttcgagttcg agatcctcga cgctgcctgc ctcggcggcc aggctgagga tggtgtcctg
     1141 tgccttcgca tgcaggatca ggcgggtgga gtcggccttg ggatcacagc cgacgatcag
     1201 gattttctgt cccatgtcgg ccaaggccgc cagggtgttt tgtgaagtgg tggatttgcc
     1261 gattccgccc ttgccgtaaa aggcgatctg ccgtagttct gccattgcca tctccttaca
     1321 aagttttggt cgtgaaaggt gcccgaattc gctgcggcct cgtggcccat gcggcagaag
     1381 accataccgg ccagagcaac tgcctttgca accccttatg gtttttcgta tcggctgcaa
     1441 atccggcaaa catcgactgc gcaatcgatc cagtgcagtc cgcgtgccat ccatgatatt
     1501 ttttctcaag cgattgaatt taaattaatt ttttaagatc tttcgaaaag ttagggtatt
     1561 cggtgattca ggcaggctgt tgcaaaggcg acaaagccgt cgactctgtc gcaaagggta
     1621 cagattggca gtctgagctc tgaaaggcgc aacaggctgt ttttcaggca tttgcaggcc
     1681 aggtcgtgat tgaaggacag gtcggaacgg aagttgctga atctggcggc aggtgttgga
     1741 gcaagaatga ccggagggtg agccatgcag gttggagtgg ggagtgccac cgcagtggaa
     1801 aacaagcggg tgttcatcgt cgacagtgac gagatcatcc gcgcggtact gcaattcatg
     1861 ctgcatgacg agtacgaggc ccatgaattg gcgaccccgg cggcggccgt ggccaaaggc
     1921 atcgactgga agcccgacct gctggtgctg ggaggcgcca ccctgcgtga gctgggtctg
     1981 gccggcttgc cgcagatcaa gtcagaactg ggcggggtga agattctgct ggtgaccgaa
     2041 gcgggagaga atgtggtggc acaagcggcg ctggatgcag gagttgccga tgcgctgctc
     2101 cttcagccgc tgacggtgga gtcggtgcgc cgcaccgtca acaggctgtg cggacgtcgc
     2161 acgcaacgca ccgtcaaact gcatcagatc tgatggcgac ggcggatctg gccgttgcga
     2221 ccagagcgcg gccgcatcgg gtcgttacgc gccgaccctg accaggacgc ggccgacgga
     2281 cttgcccgcc agcatccgtt cgatctgcgt tggcagctct tccagcgtga tctcgcgggc
     2341 gatcaggtcg agcggcaggc tgtcccagct gctggccagc cgccgccaga aggcggcctt
     2401 gatcgctggc gccagctcca ccgagtcgac gcccagcagg ttcacgccac gcagaatgaa
     2461 cggcatgatc gatgaactga gactggtcga ggagagctgc ccgcagcatg cgactgagcc
     2521 gccatagttg agcgatttga ggatctgttc gagggtgtcg ccgcccaccg tgtcgatggc
     2581 cccttgccac aggggtttca gcagtgccct gggcgagcgt tcggcgagag tgtcgcggtc
     2641 gatcacccgc tttgcaccca gtttcgacag gtagtcggcc tgctcgatct ttccggaact
     2701 ggccaccacc tcgtagccga gccgggccag cagcgccacg gccaagctgc cgacaccgcc
     2761 ggtggcaccg gtgaccagca cttcgccctg ctcgggcagc aggcccatcc gttgcagctt
     2821 gtcgacggcg atcgcggcgg tgagcccggc tgtgccgaga atcatcgctt cgcgcgggct
     2881 cagttcggtg ggcagtggaa tggcccaggc ggccggaacc cgaatgtatt cggcaaagcc
     2941 gccggcggtg ttcatgccga ggtcgtagcc agtgacgacg accggcatcc ctggtgtgaa
     3001 atcgggatgg gtggatgctt gcacgacgcc ggccgcgtcg acgccggggg tgtgcggata
     3061 gtggcgggtg atgccacggt gaccgctggc cgagagggca tccttgtagt tgagtgacga
     3121 gtagtgaacg gcgatcagca gttcaccctc cggcagttgg tcgatcgagc gggtgacgac
     3181 gctgctgccg tagaggccat cgcgaatctc gctggtttgc agggcacgga actggttcat
     3241 ggcagttcgc cggcaatctg ctgttgtaag gagagatttt cggccatggc catcgaagcc
     3301 gctggccgtg acagatgccc gcccagtcgc tgccgcagtt gccccatgct cagccccatg
     3361 cgttcaggca gcgagcgttt ttcgacatag gcgaccttga agatgtcggc ttcattcacc
     3421 tgccggccga ggtattcatc gctggtcatc agctcatcga tcagaccatg ggtgagcgcc
     3481 ttctgcccat accagacctc accggtcgcc acctgttcga tggcaagctg ggggcggtgg
     3541 ctggtgacga aatgcttgaa cagtgcatgg gtctcttcga gctcttcgat gaacttttcg
     3601 cgtcccttct cgctgttttc gccaaagagt gtcagggtcc gcttgtattc gccggcggtc
     3661 agcagttcat agtcgatctc atgttttttc agcagtcggt ggaagttggg cagttgggcg
     3721 acgacgccga tcgagccgat catggcgaaa ggggccgcca gcagccggtt ggcgatgcag
     3781 gccatcatgt agccgccgct ggcggccacc atgtcgacgc agatggtgag cgcaatgccg
     3841 tgattgcgga tgcgatcgag ttgcgaggcg gcaaaaccat aggcatgcac cattccgccg
     3901 ctgctttcga gccggaccag cacctcgtcc gccgcagtgg ccaccgccag gatcgcgctg
     3961 atctcctcgc gcagttgccg ggtttgtgat gcgcgaatgt ctcctttgaa gtcaatgaca
     4021 taaatgtgtt tttttggcag aacatcacct tttttgcgtg ccttttcgag ctgtttccgc
     4081 tcttttttcc acagcttcag agccggttca gagagcgtgg cgtggcgcag gatgtcggcc
     4141 tgctgctccc actcatggtt cagtttgcgc acctcgatct ggccgtgtgc cgccttgcgc
     4201 tggcgctgcg agagtgcggc gatgccggca accagcagca gaacgagaat gagcagggtc
     4261 agccccttgg cgagaaaaag cgcgtactcc agcagtgctg cggtcacaga tggctcctga
     4321 cagggatgaa gaacaatacg gagggccagc ggtcgtccgc cactgcctgt tattgttgca
     4381 gttgccgcag atagctttcg atcagccagc ccgaaatcga caccgaaccg ggcaccttcg
     4441 gcagattttt gtagtggaac cagcccgcct ccaccacttc gacgccatcg gccttcacct
     4501 caccggccag gtagtcggca tgaaagccca gcatcaggtt gttgggaaag ggccaggcct
     4561 gactgctgag gtagcgtgga ttggtgacct gcacgcccac ctcttcgagc acttcgcggt
     4621 agagggcctg ttcagccgac tcgcccggtt cgatgaaacc cgccagagtg ctgaacatgc
     4681 cgggtggaaa ttggggcgaa cgcccgagca gcagctcttc accgcgggtg accagcacga
     4741 tgatgcaggg tgacaggcgc gggtagttca gcagcctgca attcgggcac tccttggcac
     4801 ggtcagtcgc atggtcctgc atcgggctgg cacagcggcc gcagtagcgg tgggtgcggt
     4861 gccactgcac catctgccag gcccggctgg cggcggcaaa gaggtcgcca ttggcgaggc
     4921 tcagcagcgc gcgcaggtca gtcaggacaa agccggatgg cagcggttcg gtcgcttcgg
     4981 cggtaaagca ggggtgtccc cggaaggtgc cgatgtagtg ctcgctgtca cggtcgaggt
     5041 tgagccagcg gcattggccc tgatcgagtg gttgccaatg gggcgcctcg gtcgacatca
     5101 ccaggcgatc gccgacaaag gcgatgcagt aactctcctg gtagccatgc ggacggaggt
     5161 tgccgggttc gaactcactc aactcctgcc agaacatcgc ttctatccca ctctgcgttg
     5221 atcaccattc gggcgcaaaa ccccgtaact gcctgaagac tatcagcacg gagcggggtt
     5281 aacaatctgc atgccgatga gtcgtggcgg agcgggccat gcgctgcggc gcttcaggag
     5341 aagtcgtttc cgagtgagtc gcggatgtgg cgggggatgg gatcgaccca atgctgatag
     5401 cctgggtggt cgatgccggc ggccagtggg gcaccattgt ggatggcggc aatcatcgcg
     5461 ggggtgaatt cgaagcgcag aaaatggacc gacgaggtct tttcgctgtc cgcgcgctcc
     5521 atgtcctcgt cggcgatggc gtgaacggcg tcgaaaccgg cgaccctgat ccagacccga
     5581 ttctcgatgt cgaccagttg ggccaaggcg gcgcggcgct cgacggcatc ctcgatctcg
     5641 atcatgaagg tggccttgag gttggtgcca tccggaatca gcgggttgta ggcgtcgagt
     5701 tcatcctgaa tcgccgcgga ttcaaagatt cgctcggccc gcagcatctc ctggatctga
     5761 tactgcatcg acagccggtc ctcgaagtag agcaccgcac aggggccaat cggcagccgg
     5821 cgattccgct tgtgttcgat gatccgcgcc cggaacagcg gacgttgctc ggcatactgc
     5881 tccagcgaca gcagatcgcc gcgggtcaac tttccagcca tgtctgttca gctccgatgg
     5941 atcgtcatcg tgggttgggc catgttacca gaaccccaag cggccacgtc agccgtgccg
     6001 ctggccgctt cgaacattgc cgtttgcctg gcggggcgtc ggtttcatgc ggttgccgcg
     6061 ctgcatgatc agttccggct ggggtttgat gctcggatcg ggctcgaaac cgggcaccgt
     6121 ttcactcggc agggtgcgct tgagcagccg ttcgatgccg gtcagcagtt ttgcttcatc
     6181 gacacagacc agcgagatgg cttcgccctg ggcgccggcg cggccggtgc ggccgatgcg
     6241 gtgaatgtaa tcttccgcca cgttgggcag ttcgaagttg atcacgtgcg gcagttcatc
     6301 gatgtcgatg ccacgggcgg cgatgtcggt tgcgaccaac acctgcaact ggccaagctt
     6361 gaactccgac agggcgcggg tgcgggcgtt ctggctcttg ttgccatgga tggccagtgc
     6421 cggaatgcca cttttgtcca actgctccgc gaggcgattg gccacatgct tggtgcgggt
     6481 gaacaccagc acctgatacc actggtgctg ccggatcaga tgggtcagca gttcgcgctt
     6541 gcgctcccga tcgacccgat agaccttttg cgtcaccgca tcggccggcg cattgcgccg
     6601 cgccacctcg atatgggtgg ggctgttcag caatgtattt gccagcgttt tgatttcatt
     6661 tgagaaggtt gcagagaaga gcaggttttg ccgctttttg gggagcagcg caagaatgcg
     6721 gcggatgtca tggatgaatc ccatgtcgag catccggtcg gcttcgtcca gcaccagaat
     6781 ctccacctgc gacaggttga ccgtgcgctg ctggaggtgg tcgagcaagc gacccggcgt
     6841 ggcgaccagg atgtcgacac cgcgccgcag tcgttcgatc tgtgggttga tgccgacgcc
     6901 accaaagatc gcggttgagg tgctgcgcag atacttgccg tagccgcgga tgctctcctc
     6961 gatctgggcg gccagttcac gggtcggtgc cagaatcagc gcccgaatgg ggcggtggcc
     7021 attggcaggc gctggagtgt cagcaaggcg ttgcagaatc ggcagcgtga acccggccgt
     7081 cttgccggta ccggtctgcg cggcggcaag gagatcacca ccggccaata cggtgggaat
     7141 cgcctgctgc tgaatcggcg tcgggtcggt gtagccctgg tcggagaggg cgcgaagaat
     7201 ttcattggca aggccgaggg tggcaaaact catgggattc ctgaaagtgg gaaagacaaa
     7261 gcatcctgca tcgtcatcga tgcagcaaag cggcatgggt tggatcgcga gacggcgaca
     7321 gtgaggtgaa tggcgggcaa ctgctggttg caggcattgc gtggacgccg ccatcatacc
     7381 acactctctg ctgcgcggcc gttgtatccg attcgggggt ggagcggttt gccggcggtc
     7441 tcttgacgct ggcgcagcgc ttcactacat tgccgccttc gcgctgctgg cccgacgcag
     7501 gcggatgaag gtccccttcg tctagtggcc taggacaccg ccctttcacg gcggtaacag
     7561 gggttcga
//
LOCUS       DAORCQ010000107         7521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_6948_length_7521_cov_5.777525, whole genome shotgun sequence.
ACCESSION   DAORCQ010000107 DAORCQ010000000
VERSION     DAORCQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_7521_cov_5.777525"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKI23_09920"
     CDS             <1..536
                     /locus_tag="PKI23_09920"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNN37291.1"
                     /translation="KTGGDGLATSDQLGMYNGKIDRYDWKLVGKKEMFIPYNAYMLHT
                     DKVKVADVIRPGHINQDVARYELHRVWHIEGDLKPDTSHIYKKRVFFMDEDSWQIAVM
                     DHYDKRDQLWRMTEGHVINSYDILTATNTAEIHYDLQSGRYLAVGLDNEDTPPDNSFR
                     AGPERYTPDALRQEGVR"
     gene            724..1650
                     /locus_tag="PKI23_09925"
     CDS             724..1650
                     /locus_tag="PKI23_09925"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNN37292.1"
                     /translation="MLATLFSLSSTLPAFAAESLQRPSTASPLSSKTLLTDSTLAGQR
                     IVAVGTRGHIVYSDDQGKSFSQAAVPTRMLLTAVDFVSDQEGWAVGHDAVILHTRDGG
                     NSWTLQHEDASLEKPLLDVLFLDAQHGIAVGAYGLMLKTSDGGSSWEAAEVEEADWHF
                     NALIKIKEGLLIVGEAGNLLRSDDMGETWTALESPYVGSLFGANSVGPDQQILIYGLR
                     GHIYQSNDRGDSWEELKTDLTRNLFDSAELPNGDVVVVGGGGTVLVKASADQQFKKVP
                     YPGYGNLVSVLPLSDTEVLLFGARGAQPLKVR"
     gene            1696..4101
                     /locus_tag="PKI23_09930"
     CDS             1696..4101
                     /locus_tag="PKI23_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNN37293.1"
                     /translation="MLRDRFTLLTEKLVFANRVAILVVFALITAFMGYKAMSLRMEAG
                     FVKLLPYKHPYMRTLIDYSAKFGGGNQIIVALMQKKGDIFTPEFFTTLHEASDEVFFL
                     PGVDKSKVTSIFTPNVRFTEFVEDGFAGGNVIPAEFQPTPEWLEIARQNIIKSGELGR
                     LVTFDFTGSLIRAELLDRDPITGKKLDYQQVSKDLEEKIRQRFENDNVSVHILGFAKS
                     IGDIAEGARSVIAFFGIAFVVTGVLLWLYSGSIQLTLMPLLCSSVAVIWQLGTLSLFG
                     FGIDPMSILVPFLIFAIAVSHSVQMISGWIGEVLYGADEQDVHHPVVPVNAKTQGVDG
                     YSAARRTFRRLLIPGSIAVLCNILGFVTILMINIGIIQEMGITASIGVTIIILTNLLL
                     LPILLSFINLPNIEQYRQKRVAAESKRDGLWRTLSKFATTAWSIPTLVIIAITTVMAH
                     FYSQGLQVGDLHAGVSELREEARYNLDTKMVTSKFAIGVDVLSVIVETPKNACIDYDT
                     MVAMDELHWFAQNLPGVQSVLSLSEAMKIVTGGNNEGSPRWETLMRNQYNLSGALRYM
                     ETSSGLMDAECTAMPLLIYTKDHKADTVETIVRALEKYDSGLPKEKFNPRLASANVGI
                     IAATNQAVHAAQEPIMAYVHGAIALLVLLSFRSVMVTACLMIPLLYVSSLCYALMTYL
                     NIGLKVNTLPVVALGIGIGVDYGIYLYSRMADFLNEGLGLQESLYRALKLTGKPVMFT
                     AATLAVGVATWIFSDLKFQADMGILLTFMFIVNMIGAILVIPALARWLLPARLRNPST
                     PAG"
     gene            complement(4313..6220)
                     /gene="htpG"
                     /locus_tag="PKI23_09935"
     CDS             complement(4313..6220)
                     /gene="htpG"
                     /locus_tag="PKI23_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNN37294.1"
                     /translation="MSAPQQAQTYGFQTEAKQLLHLMIHSLYSNKEIFLRELISNASD
                     AADKLRFAALADSSLYENDPELRIWIDCDADARTVTVTDNGIGMSRDEVIAHLGTIAK
                     SGTAEFLRSLTGDQRKDSSLIGQFGVGFYSAFIVADRVEVFSRKAGLPAQQGVHWQSQ
                     GESEFSVAPVELPQRGTRVVLHLKEDEAEFSEHFRLRHIIKTYSDHIALPVMMRAEAT
                     GDAEEQPSETPDYEQVNVAKALWSRPRNEIDEAEYQEFYKHVAHDFENPLAWSHNRVE
                     GKLEYTSLLYLPRRAPYDLWNRDRQRGLKLYVQRVFIMDDAEQFLPLYLRFIKGVIDS
                     NDLSLNVSREILQKDPVIDSMRAALVKRVLDMLDKLAEEQPQEYQTFWEEFGTVLKEG
                     LAEDHANRERIAALMRFASTQGDGARQVRSLKEYLAAMKPAQQQIYYLTAESFAAGSN
                     SPHLEIFRKKGVEVLILSDRIDEWVMGHLHQFEGKQFQDVSRGDLDLTALEDEADKSA
                     QQAQAKEYETLLERVKAQLAERVKAVRLTHRLTDSPACLASDEADLGGQMRRMMQAVG
                     QKLPESTPYFELNPDHPLIARLKQQPEGERFADLVEILFDQARLANGEQLAEPGRYSA
                     RLNRLLSDLLG"
     gene            complement(6441..7313)
                     /gene="sucD"
                     /locus_tag="PKI23_09940"
     CDS             complement(6441..7313)
                     /gene="sucD"
                     /locus_tag="PKI23_09940"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNN37295.1"
                     /translation="MSILIDKDTKVLCQGFTGAQATLHSEQALDYGTRLVGGVTPNKG
                     GQTHLGLPVFNTMREAVEATAADASVIYVPAPFCKDSILEAAHSGVKLVVCITEGIPT
                     LDMLQCKLVCDRLGVRLIGPNCPGVITPGECKIGIMPGDIHLPGKVGIVSRSGTLTYE
                     AVKQTSDFGFGQSSCVGIGGDPIPGSSFIDILGLFQADPGTEAIVMVGEIGGSAEEEA
                     ARFIKQHVTKPVVSYIAGVTAPAGKRMGHAGAIISGGKGTAAEKFAALEAAGVRTVRS
                     LADIGKAIAEVTGW"
     gene            complement(7310..>7521)
                     /gene="sucC"
                     /locus_tag="PKI23_09945"
     CDS             complement(7310..>7521)
                     /gene="sucC"
                     /locus_tag="PKI23_09945"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701527.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNN37296.1"
                     /translation="GIVRCDLIAEGVIGAVEEVGVKIPVVVRLEGNNARLGAQILADS
                     GLNIIAAQSLTDAAQQVVKAAERQP"
BASE COUNT         1385 a         2421 c         2274 g         1441 t
ORIGIN      
        1 ccaagaccgg cggtgatggc ctggccacca gcgaccagtt gggcatgtac aacggcaaga
       61 tcgaccgcta cgactggaag ctggtcggca agaaggagat gttcatcccc tacaacgcct
      121 acatgctcca caccgacaag gtgaaggtgg ccgatgtcat ccgcccgggt cacatcaacc
      181 aggatgtggc ccgctatgaa ctgcaccggg tctggcacat cgagggcgac ctgaagcccg
      241 acaccagcca catctacaag aagcgggtct tcttcatgga cgaagacagc tggcagatcg
      301 ccgtcatgga ccactacgac aagcgcgatc aactgtggcg gatgaccgag gggcatgtga
      361 tcaactccta tgacatcctg accgccacca acactgccga gatccactac gacctgcagt
      421 cagggcgcta tctggcggtc ggcctcgaca acgaggacac cccgcccgac aacagcttcc
      481 gtgccggtcc tgagcgctac acccccgacg cattgcgcca ggagggcgtg cgctgagctt
      541 gcacgcatga tgtcctgatg cctgcatccg gatcagccga tccggatgca ggttgaccta
      601 aaaacggaag ccgtcgagca gcccgctccg ggtcgatcca cctcgacgca gacccaacaa
      661 taacgactcc acaaaggaga tttctccatg gaccaccggc caaggctgcg cacgtggttt
      721 tgtgtgcttg caacgctgtt ctcgctctca tccacgctgc cagcattcgc agccgaatcg
      781 ctgcagcgcc catccaccgc ctcgcccctc tccagcaaga cactgctgac tgattcgacg
      841 ctggccggcc agcggatcgt cgcggtcggc acccgtggcc acatcgtcta cagcgacgac
      901 cagggcaaga gcttcagcca ggcagcggtc cccacccgga tgctgctgac ggcggtggac
      961 ttcgtctccg atcaggaggg ctgggccgtc ggtcatgatg cggtgatcct gcacacccgc
     1021 gatggcggca acagctggac gctgcaacat gaggatgcct cgctcgaaaa gccgctgctc
     1081 gatgtgctgt ttctggatgc ccagcatggc atcgcggtcg gcgcctatgg cctgatgctg
     1141 aagaccagcg acggcggcag cagttgggaa gccgcagagg tcgaggaggc cgactggcac
     1201 ttcaacgcgc tgatcaagat caaggagggt ctgctgatcg tcggcgaagc gggcaatctg
     1261 ctgcgctccg atgacatggg cgaaacctgg accgcgctcg aatcgcccta cgtcggttca
     1321 ctgttcggcg ccaacagcgt cgggcctgat cagcagattc tgatctacgg cctgcgcggt
     1381 catatctacc agtcgaacga ccgcggtgat tcgtgggaag agctgaagac cgacctgacc
     1441 cgcaacctgt tcgacagcgc cgagttgccg aatggcgacg tggtggtcgt gggcggtggc
     1501 ggcaccgtgc tggtgaaggc atccgctgat caacagttca agaaagtgcc ctatcccggt
     1561 tatggcaacc tggtctccgt gctgccgctg agtgacaccg aggtgctgct gttcggcgcc
     1621 cgcggtgccc agccgctcaa ggtcagataa caacaaccgc cgtcattgcg aacggaccaa
     1681 cggaaggatt ttgtcatgct aagagatcga ttcaccctgc tgacggaaaa actggttttc
     1741 gccaatcgtg tggcgattct ggtggtcttc gcgctcatca ccgccttcat gggctacaag
     1801 gcgatgagcc tgcgcatgga ggccggcttc gtcaaactgc tgccctacaa gcacccctac
     1861 atgcgcacgc tgatcgatta cagcgccaag ttcggcggtg gcaaccagat catcgtcgca
     1921 ttgatgcaga aaaaaggtga catcttcaca ccggagttct tcaccaccct gcacgaagcg
     1981 tcggatgagg tcttctttct gcccggggtc gacaagtcga aggtgacttc gatcttcacc
     2041 cccaatgtgc gcttcaccga attcgtcgag gatggctttg ccggtggcaa cgtgattccg
     2101 gccgagttcc agccgacacc ggagtggctg gagatcgccc gccagaacat catcaaatcg
     2161 ggcgaactgg gccgtctggt caccttcgac ttcaccggct cgctgattcg tgccgaactg
     2221 ctcgatcgcg atccgatcac cggcaagaag ctcgactatc agcaggtctc caaggatctc
     2281 gaagagaaga ttcgccagcg cttcgagaac gacaacgtca gcgtgcacat tctcggcttt
     2341 gccaaatcga tcggtgacat cgccgaaggt gcccgcagcg tgatcgcctt cttcggcatc
     2401 gccttcgtcg tcaccggggt gctgctgtgg ctctactccg gctcgattca gctcaccctg
     2461 atgccactgc tctgctcgtc ggtggcggtg atctggcagc tcggcacatt gagcctgttc
     2521 ggcttcggca tcgacccgat gagcatcctg gtgccctttc tgatcttcgc catcgccgtc
     2581 agtcacagcg tgcagatgat cagcggctgg atcggcgagg tgctttacgg cgccgacgag
     2641 caggatgtcc accatccggt ggtgccggtc aatgccaaga cccagggcgt cgatggctac
     2701 agtgcggccc gccgcacctt ccgccgcctg ctgatccccg gcagcatcgc ggtgctctgc
     2761 aacattctcg gtttcgtcac catcctgatg atcaacatcg gcatcatcca ggagatgggc
     2821 atcaccgcca gcatcggcgt gaccatcatc atcctgacca acctgctgct gttgccgatc
     2881 ctgctgtcgt tcatcaacct gcccaacatc gagcagtacc ggcagaagcg ggtggcggca
     2941 gagagcaagc gcgacgggct gtggcgcacc ctctcgaagt tcgcaaccac cgcctggtcg
     3001 attccgacgc tggtcatcat cgccatcacc acggtcatgg cgcacttcta cagccagggt
     3061 cttcaggtcg gcgacctgca tgccggggtc tctgaactgc gcgaggaggc ccgctacaac
     3121 ctcgacacca agatggtcac cagcaaattc gccatcggtg tggatgtgct gtcggtgatc
     3181 gtcgagacgc cgaagaatgc ctgcatcgac tatgacacca tggtcgcgat ggatgaactg
     3241 cactggttcg cacagaacct gccgggcgtg cagtcggtgc tgtcactgtc ggaagcgatg
     3301 aagatcgtca ccggtggcaa caacgagggg tcaccgaggt gggagaccct gatgcgcaac
     3361 cagtacaacc tgtcgggtgc gctgcgctac atggagacca gcagcgggct gatggatgct
     3421 gaatgcactg ccatgccgct gctgatctac accaaagacc acaaggccga cacggtggag
     3481 accatcgtcc gcgcactgga gaagtatgat tccggcctgc cgaaggagaa gttcaatccg
     3541 cggctggcct cggccaacgt cggcatcatc gccgccacca accaggccgt gcatgcggca
     3601 caggagccga tcatggccta tgtccatggc gcgatcgccc tgctggtgct gctgagcttc
     3661 cgctcggtga tggtcaccgc ctgtctgatg attccgctgc tctatgtctc ttcgctctgc
     3721 tatgcgctga tgacctatct gaacatcggt ctcaaggtga acacgctgcc ggtggtggca
     3781 ctggggatcg gcatcggggt cgactacggc atctacctct acagccggat ggccgacttc
     3841 ctgaacgaag gtctgggact gcaggagtcg ctctaccgcg ccctgaagct gaccggcaag
     3901 ccggtgatgt tcaccgccgc aacgttggcg gtcggcgtgg ccacctggat attctccgac
     3961 ctgaagttcc aggctgacat gggcattctg ctcaccttca tgttcatcgt gaacatgatc
     4021 ggcgccatcc tggtgatccc ggccctggca cgctggctgc tgcccgccag gctgcgcaat
     4081 ccgagcacgc cagccggctg accaccgcgc ttcacccaaa aaagaacccg gccaaggccg
     4141 ggttcttttt tgggtttctc tcattgatcc ggtcagaaga agtctggagc atgtccctct
     4201 accccagccc ctctcccgca tgcgggagag ggcggattca gacatgtgcg ttccggatcg
     4261 atctgaaacc gcgcccgcct ccttgcttgc ggcggcggcg ctgtttcaag gctcacccca
     4321 gcagatcact cagcaaccga ttgagccgcg cactgtaacg gcccggctcg gccagctgct
     4381 caccgttggc caggcgtgcc tgatcgaaca ggatctcgac caggtcggca aagcgctcac
     4441 cctcgggctg ctgcttcagt cgggcgatca gcggatggtc gggattgagc tcgaagtaag
     4501 gggtgctctc gggcagcttc tggccgacgg cctgcatcat ccgccgcatc tggccaccca
     4561 ggtcggcttc gtcactggcg agacaggctg gcgagtcggt cagccggtgg gtcaaccgca
     4621 ccgccttgac ccgctcggca agctgtgcct tgacccgctc cagcaatgtc tcgtactcct
     4681 tggcctgggc ctgctgcgca ctcttgtcgg cctcgtcctc cagcgccgtc aggtcgaggt
     4741 caccacgcga gacatcctgg aactgcttgc cctcgaactg atgcaggtgg cccatcaccc
     4801 attcatcgat ccggtcggac agaatcagca cctcgacccc cttcttgcgg aagatctcca
     4861 gatgggggct gttgctgccg gcagcgaagc tctcggcggt caggtagtag atctgctgct
     4921 gtgccggttt cattgccgcc agatactctt tcagcgaacg cacctgccgc gcgccatcgc
     4981 cctgggtcga ggcaaagcgc atcagcgcgg cgatgcgttc acggttggcg tggtcctcgg
     5041 ccagtccctc tttcagcacg gtgccgaact cttcccagaa ggtctgatat tcctgcggct
     5101 gctcctcggc cagtttgtcg agcatgtcga gcacccgctt gaccagcgcc gcgcgcatcg
     5161 agtcgatcac cggatccttc tgcagaatct cccgcgagac gttcagcgag aggtcattgg
     5221 agtcgatcac ccccttgatg aagcgcaggt agagcggcag aaactgctcg gcatcgtcca
     5281 tgatgaagac gcgctgcacg tagagcttga gtccacgctg gcggtcgcgg ttccacaggt
     5341 cgtagggcgc acgccgcggc agatagagca ggctggtgta ttcgagcttc ccctcgaccc
     5401 ggttgtggct ccaggccagc gggttctcga agtcgtgggc gacatgcttg taaaactcct
     5461 gatactcggc ctcgtcgatc tcgttgcgcg gccggctcca gagcgccttg gccacattga
     5521 cctgctcata gtcgggcgtt tcactcggct gctcctctgc atcaccggtc gcctcggccc
     5581 gcatcatcac cggcagcgca atgtggtcgg agtaggtctt gatgatgtgc cgcaggcgaa
     5641 agtgctcgga aaattccgcc tcatcctcct tcagatgcag caccacccgc gtgccgcgct
     5701 gcggcaattc gaccggtgcg accgagaatt ccgactcgcc ctgtgactgc caatgcaccc
     5761 cctgctgcgc cggaagaccc gccttgcggc tgaacacctc cacccggtcg gccacgatga
     5821 aggccgagta gaagccgaca ccgaactggc cgatcaggct ggagtccttg cgctggtcgc
     5881 cggtcagcga gcgcagaaac tccgcggtgc ccgacttggc gatggtcccg agatgggcga
     5941 tcacctcgtc acggctcatg ccgatgccgt tgtcggtcac ggtcacggtg cgcgcgtcgg
     6001 catcgcagtc gatccagatc ctgagctcgg gatcgttctc gtagagcgaa ctgtcggcga
     6061 gggcggcaaa acgcagcttg tccgcggcgt cggaggcgtt ggagatcagc tcgcgcagaa
     6121 agatctcctt gttgctgtag agcgaatgga tcatcagatg aagcagttgc ttcgcttcgg
     6181 tctggaatcc gtaggtttgc gcttgctgtg gtgcactcat gtggtcagac gactccttgc
     6241 agggcgcgga ggcagtgccc ggccgcgctg ttcgaacgat ggatttgact ggggaatatg
     6301 ggggagttgc cgggcttttc aagccgatcg tccgctgcat ggcccagccg caggttcgac
     6361 tcgatgttga ctggaccgta acgaaagggg cggtcaccct gcggcaaccg ccccttcgat
     6421 tcccagcggc gtcacgcgct ctaccagccg gtcacctcgg caatcgcctt gccgatgtcg
     6481 gccagactgc gcacggtgcg cacaccggct gcttcgagcg cggcgaactt ttcggccgca
     6541 gtgcccttgc cgccggagat gatggcgccg gcgtggccca tccgcttgcc ggctggcgcg
     6601 gtgacgccgg cgatgtagga gaccaccggt ttggtgacat gctgcttgat gaagcgggca
     6661 gcctcctcct cggcgctgcc accgatctcg ccgaccatga cgatcgcctc ggtcccgggg
     6721 tcggcctgga acagccccag gatgtcgatg aagctgctgc ccggaatcgg atcgccaccg
     6781 atgccgacgc aactgctctg gccaaagccg aagtcgctgg tctgcttgac cgcctcatag
     6841 gtgagggtgc cggagcggga gacgatgccg accttgccgg gcagatggat gtcgccgggc
     6901 atgatgccga tcttgcactc gccgggggtg atgacaccgg gacagttggg gccgatcagc
     6961 cggacaccca gccggtcgca caccagcttg cactgcaaca tgtcgagggt cgggatgccc
     7021 tcggtgatgc agaccaccag cttgacgccg gagtgggccg cttcgaggat cgaatccttg
     7081 cagaacggcg ccggcacgta gatgaccgaa gcatcggcgg ccgtcgcctc gaccgcctca
     7141 cgcatggtat tgaacaccgg caggcccaga tgggtctggc cgcccttgtt cggcgtcacg
     7201 ccgcccacca gtcgggtgcc atagtcgagc gcctgctcgg aatggagcgt cgcctgcgcg
     7261 ccggtgaagc cctggcagag caccttggtg tctttgtcga tcagaatgct catggctgac
     7321 gctccgcggc cttgacgacc tgttgtgctg cgtcggtcag actctgtgca gcaatgatgt
     7381 tgaggccgct atcggccagt atctgtgcgc ccagtctggc gttgttgccc tccagccgca
     7441 ccacgaccgg aatcttgacg cccacctcct cgaccgcacc gatcaccccc tcggcgatca
     7501 ggtcgcagcg cacgatgccg c
//
LOCUS       DAORCQ010000108         7440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_7060_length_7440_cov_5.185376, whole genome shotgun sequence.
ACCESSION   DAORCQ010000108 DAORCQ010000000
VERSION     DAORCQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7060_length_7440_cov_5.185376"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /gene="ureC"
                     /locus_tag="PKI23_09950"
     CDS             <1..1582
                     /gene="ureC"
                     /locus_tag="PKI23_09950"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNN37297.1"
                     /translation="EVKFGGGKVIRDGMGQSQASSKIAMDTVITNAVILDHWGIVKAD
                     IGLKAGRIHAIGKAGNPDIQPGVTIVIGPGTEVIAGEGLIVTAGGIDAHIHFICPQQI
                     DEALMSGVTTMLGGGTGPATGTNATTCTPGPWHLARMLQALEPFAMNFGLLGKGNASL
                     PEALEEQLLAGAMGLKLHEDWGTTPAAIDCCLAVADRFDIQVAIHTDTLNESGFVEET
                     LAAFKGRTIHTFHTEGAGGGHAPDIIKACGEPNVLPSSTNPTRPYTVNTVDEHLDMLM
                     VCHHLDPGIPEDVAFADSRIRKETIAAEDILHDLGAFSMISSDSQAMGRVGEVITRCW
                     QSAHKMKVQRGALAEDSARHDNFRVRRYVAKYTINPAIAHGIAHVVGSVEPGKLADLV
                     LWRPAFFGVKPAMILKGGMIAAAPMGDPNASIPTPQPVHYRPMFGAFGSAPHHTSLTF
                     VSRAALEAGIKERLDLNRTLVAVGNTRQIGKAQMVHNSYLPRMEVDPQTYEVRADGLL
                     LSCEPASVLPLAQRYFLF"
     gene            1604..2056
                     /gene="ureE"
                     /locus_tag="PKI23_09955"
     CDS             1604..2056
                     /gene="ureE"
                     /locus_tag="PKI23_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004576554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNN37298.1"
                     /translation="MSTLTQVSELLGHHHHDQADARLILDFEARSKSRQRVQLADGEA
                     VALMLPRGTLLRGGDWLRDSRGRLIEVVAAPEWLSCVSGEPLQLLRAAYHLGNRHIPL
                     QIAPGWLRYQHDSVLDQMVIGLGLTLDRQQLPFEPEAGAYQGGHHHAD"
     gene            2046..2732
                     /locus_tag="PKI23_09960"
     CDS             2046..2732
                     /locus_tag="PKI23_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNN37299.1"
                     /translation="MPTELARLRLLQLTSPALPIGAFACSQSLESAVTLGWVDDADSL
                     ADWIGGLLSHGLGRLDLPILARQIQACAADQQAEAVRWNRHLLASRESGELRLEDRQL
                     GAALLRLLTTLEGADVLDWLADEAACFGVAQVTLFGWAAARFGIEPAAALEGFAFAWL
                     ENQLTAATKLMPLGQSRAQRLLSGFLPRLPALVEQALQCDDSAIGAALPGMAFASGRH
                     ESQYCRLFRS"
     gene            2750..3379
                     /gene="ureG"
                     /locus_tag="PKI23_09965"
     CDS             2750..3379
                     /gene="ureG"
                     /locus_tag="PKI23_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNN37300.1"
                     /translation="MSSAQALRVGVGGPVGSGKTALLARLCRLLRDHYQIAVVTNDIY
                     TQEDAQFLVRNEALAPERIIGVETGGCPHTAIREDASMNLQAVAELQQRFSDLELIFV
                     ESGGDNLSATFSPELSDLTLYVIDVAAGDKIPRKGGPGITRSDLLIINKTDLAPLVGA
                     DLAVMERDAKKMRGERPFLFTNLKQGDGVAAVVDFIVTAGLLPAPRLPQ"
     gene            3376..4020
                     /locus_tag="PKI23_09970"
     CDS             3376..4020
                     /locus_tag="PKI23_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37301.1"
                     /translation="MITDGSTLQIMTLAMGLGALHALDADHLAVVTSLGSRRPGWRHA
                     LGFTLRWALGHALSLIALATLLLVTARNFAEQLGGTAEQLAGFSLIAIALWLLAHPLH
                     PSVDRAAFHNGDPPRPNRSPLLATALGLLHGVAGSAPILALIPLTQLQRPVEMLYFVL
                     LFSSGVVLSMCLVGFLIGHFWQRIHHRSHGVARLLHRLVACTTLAIGARLLLVH"
     gene            complement(4044..4424)
                     /locus_tag="PKI23_09975"
     CDS             complement(4044..4424)
                     /locus_tag="PKI23_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37302.1"
                     /translation="MQSNSASEGILRSGSHHRGHPMHIRWKTACSTIVLLSLSLGLCA
                     APKGGHGPAEHDHAATPPASSNRQSLPEAERGAERAQERQSEQAQEHSRAHEQQMEQE
                     LEQHKTQHSHGQGSQENKENKKLD"
     gene            4522..4953
                     /gene="trxC"
                     /locus_tag="PKI23_09980"
     CDS             4522..4953
                     /gene="trxC"
                     /locus_tag="PKI23_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNN37303.1"
                     /translation="MLQINCPHCFVTNRLPAQRLGDRPNCGRCKQPLFTGTPLELTEA
                     NLTATLTRNDIPLVVDCWAAWCGPCRSFAPTFSQAAARYEPHARFAKLDTEALPAVAQ
                     QWAIRSIPTLILFRNGQEVQRQAGALSPPQLAQWLKQAGVV"
     gene            complement(5039..6052)
                     /locus_tag="PKI23_09985"
     CDS             complement(5039..6052)
                     /locus_tag="PKI23_09985"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN37304.1"
                     /translation="MSTPVLTFFNNKGGVGKTSLVFHVAWMLCELGKRVVAIDLDPQA
                     NLTSAFLAEETLETLWDPPAPVESGTTIFRCVQPLTKVGDIRPPVVQSIHPGLVLVPG
                     DLALAGFEDELSKAWLEAMGSNNLYRPFRLLTAFWQVAQMAAAEHEADLILADVGPNL
                     GAINRSALIGSDHVVIPLAADLFSLQGLRNLGPTLTAWRSDWQKRLNNWPEPEFLLPA
                     GRMAPLGYILMQHMERLSRPVKAYKKWVDRIPATYVETVTGVQSPTPAHADDGNCLAR
                     LKHYRSLIPMAQETRKPVFHLTSADGAIGSHSQAVRDAGNDFRALATTILQRIDRGEE
                     SAF"
     gene            complement(6049..7263)
                     /locus_tag="PKI23_09990"
     CDS             complement(6049..7263)
                     /locus_tag="PKI23_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN37305.1"
                     /translation="MTPLTDIELAALADDLESDRVERKRAWKGDAPEKGRQAVCAFAN
                     DLPNHRKPGVLLVGIADDGTPAGPEVVVTDALLQTLADIKTDGKITPPPTLTVTRRFL
                     KGHDVAVVTVWPADAPPVRLDGRIWIRTGPRRGLASAQDERILNERRRHLDRAFDATP
                     VYGCPLDELDRLTFEREFLPQAIAPDVLAANDRSYEDRLASLGMIASAADPTPTVAGI
                     LTLGKSPRTWVPCAYIQFLRIRGTELADPIADAAELDGTLQQMLRRADEKLAAFLTTE
                     VDFTSATIEERRTPYPMSALQQLLRNAVMHRTYEGTNAPVRLYWFDNRIEIHSPGGPY
                     GVVNAANFGQPGACDYRNQILAGVLKVLGFVQRFGFGIAQARRALAANGNPPPEFRVE
                     PTMVLATLRRAP"
BASE COUNT         1249 a         2504 c         2401 g         1286 t
ORIGIN      
        1 ggaggtgaag ttcggcggcg gcaaggtgat ccgcgacggc atgggccaga gccaggccag
       61 cagcaagatc gccatggaca cggtgatcac caatgcggtg atcctcgacc actggggcat
      121 cgtcaaggcc gacatcggcc tgaaggccgg gcgcatccat gccatcggca aggccggcaa
      181 ccccgacatc cagcccgggg tgacgatcgt catcggtccc ggcaccgagg tgattgccgg
      241 cgaggggctg atcgtcaccg ctggcggcat cgacgcccac atccatttca tctgcccgca
      301 gcagatcgac gaggcgctga tgtccggcgt caccaccatg ctgggtggtg gcaccggacc
      361 ggccaccggc accaatgcca ccacctgcac gccgggaccc tggcatctcg cccgcatgct
      421 gcaggcgctg gagccctttg cgatgaactt cggcctgcta ggcaagggca acgccagcct
      481 gcccgaggcg ctcgaggagc agctgctggc cggcgcaatg ggactgaaac tgcacgagga
      541 ttggggcacc accccggcgg cgatcgactg ctgtctggcg gtggccgacc gcttcgacat
      601 ccaggtggcg atccacaccg acaccctcaa cgagtcaggc tttgtcgaag agaccctggc
      661 cgccttcaag gggcgcacca tccacacctt ccacacggaa ggggccggcg gcggccatgc
      721 cccggacatc atcaaggcct gtggcgagcc caatgtgctc ccctccagca ccaatccgac
      781 ccgtccctat accgtcaata ccgtcgatga gcacctcgac atgctgatgg tctgtcatca
      841 cctcgatccc ggcattcccg aggatgtcgc cttcgccgac tcgcgcatcc gcaaggagac
      901 catcgccgcc gaggacatcc tgcatgacct cggtgccttt tcgatgatct cctccgactc
      961 gcaggcgatg ggccgggtcg gcgaggtgat cacccgctgc tggcagagcg cgcacaagat
     1021 gaaggtgcag cgcggcgcgc tggccgaaga cagcgcccgc cacgacaact ttcgtgtgcg
     1081 ccgctatgtc gccaagtaca ccatcaaccc ggccattgcc catggcatcg cccatgtggt
     1141 cggctcggtc gaaccgggca agctggccga cctggtgctg tggcggccgg ccttcttcgg
     1201 cgtcaagccg gcgatgatcc tgaagggcgg aatgatcgcc gccgcgccga tgggcgaccc
     1261 caatgcctcc attcccaccc cgcagccggt ccactaccgg ccgatgttcg gtgccttcgg
     1321 cagcgcgccg caccacacca gcctcacctt cgtctcgcgg gcggcgctcg aggcaggcat
     1381 caaggagcgg ctcgacctga accgcaccct ggtcgcggtc ggcaacaccc gccagatcgg
     1441 caaggcgcag atggtccaca acagctacct gccaagaatg gaagtggacc cgcaaaccta
     1501 cgaggtgcgc gccgatgggc tgctgctcag ttgcgaaccg gccagcgtgc tgccgctggc
     1561 acagcgctac tttctgttct gaacaaagcc gggaggcttg ccgatgtcca ccctcaccca
     1621 ggtcagcgag ctgctgggtc atcaccacca cgatcaggcc gatgcccggc tgattctcga
     1681 cttcgaggcg cgcagcaaga gccgccagcg ggtccagttg gccgatggcg aggcggtcgc
     1741 gctgatgctg ccgcgcggca cgctgctgcg cggcggcgac tggctgcgcg acagccgggg
     1801 ccggttgatc gaggtggtcg ccgcgccgga gtggctcagt tgcgtcagcg gcgaaccctt
     1861 gcagctgctg cgcgccgcct accacctcgg caaccgccac atcccgttgc agatcgctcc
     1921 cggctggctg cgctatcagc acgacagcgt gctcgaccag atggtgatcg gcctggggtt
     1981 gaccctcgac cggcagcagt tgccgttcga gcccgaagcc ggcgcctacc agggcggcca
     2041 ccaccatgcc gactgaactg gcgcggctgc ggctgctgca actgaccagc ccggccctgc
     2101 cgatcggcgc ctttgcctgc tcccagtcac tcgaaagtgc ggtaaccctc ggctgggtcg
     2161 atgatgccga cagcctggcc gactggatcg gcgggctgct gagccatggg ctgggccggc
     2221 tcgacctgcc gattctggcc cggcagattc aagcctgcgc ggccgatcag caggccgagg
     2281 cagtgcgctg gaaccgccat ctgctcgcca gccgcgagag cggcgagctg cggctcgaag
     2341 accggcaact gggcgcggcc ctgctgcggc tgctgaccac gctcgaaggt gccgacgtgc
     2401 tcgactggct ggccgacgaa gccgcctgct tcggtgtcgc ccaggtcacg ctgttcggct
     2461 gggccgccgc ccgtttcggc atcgaaccgg ctgccgcgct cgaagggttc gccttcgcct
     2521 ggctcgaaaa ccagctcacc gctgccacca agttgatgcc actggggcag agcagggcgc
     2581 agcggctgct gagcggtttt ctgccacggt tgcccgcgct ggtcgaacag gcgttgcagt
     2641 gtgacgacag cgccatcggc gctgcgctgc cgggcatggc ctttgccagc ggccgccacg
     2701 aaagccagta ttgccgattg ttccgttcat gaatcgagga gtttccacca tgtccagcgc
     2761 tcaagccttg cgggtcggcg tcggcggtcc ggtcggctcc ggcaagaccg cgctgctggc
     2821 gcggctctgc cgtctcctgc gcgaccacta ccagatcgcc gtcgttacca atgacatcta
     2881 cacccaggag gatgcccagt ttctggtgcg caacgaggcg ttggcgccgg aacggatcat
     2941 cggcgtcgaa accggtggct gtccccacac cgcgatccgc gaggatgcct cgatgaacct
     3001 gcaggcggtg gccgaactgc aacagcgttt cagcgacctc gaactgatct tcgtcgaaag
     3061 cggcggtgac aacctgagcg ccaccttcag cccggagctc tccgacctga ccctctatgt
     3121 gatcgacgtc gcggccggtg acaagattcc ccgcaagggc ggacccggca tcacccgctc
     3181 cgacctgctg atcatcaaca agaccgacct cgccccgctg gtgggcgccg atctggcggt
     3241 gatggagcgc gacgcaaaga aaatgcgtgg cgagcggccg tttctgttca ccaacctcaa
     3301 gcaaggcgac ggcgtcgccg cggtggtcga cttcattgtc accgccggcc tgctgcccgc
     3361 cccccggctg ccgcagtgat caccgacggc agcaccctgc aaatcatgac gctggccatg
     3421 gggctgggcg cgctgcacgc gctcgatgcc gaccacctgg ccgtggtcac ctcactgggc
     3481 agccgccggc ccggctggcg ccatgccctc ggcttcacgc tgcgctgggc gctgggccat
     3541 gccctgtcgc tgatcgcgct ggccaccctg ctgctggtca ccgcgcgcaa cttcgccgag
     3601 cagctcggcg gcacggccga acagttggcc ggcttcagcc tgatcgccat tgccctgtgg
     3661 ctgctggcgc atccgctgca tcccagcgtc gatcgggcag ccttccacaa cggcgaccca
     3721 ccgcggccga accgctcccc gctgctggcc accgcccttg gcctgctgca tggcgtggcc
     3781 ggttcggcgc cgattctggc gctgattccg ctcactcaac tgcaaagacc ggtcgagatg
     3841 ctctacttcg tactgctgtt cagcagcgga gtggtgctgt cgatgtgcct ggtcggcttt
     3901 ctgatcggcc acttctggca gcggattcat caccgcagcc atggcgtggc ccggctgctg
     3961 caccggctgg ttgcctgtac caccctggca atcggtgcgc gactgctgct cgtccactga
     4021 gcggcagccg cgccggagct gcctcagtcg agctttttat tctccttatt ctcctggctg
     4081 ccctggccat gagaatgctg cgttttgtgc tgttccaatt cctgctccat ctgctgctca
     4141 tgggcacggg agtgctcctg agcctgttcg gactgccgct cctgcgcccg ttccgcaccc
     4201 cgctccgcct cgggcaacga ctggcgattg ctgcttgccg gtggcgtggc ggcgtggtca
     4261 tgctcggccg ggccatgacc tcctttgggt gcggcgcaga gcccaaggct cagactcaaa
     4321 agcacaatgg tgctgcaggc ggttttccaa cgaatgtgca tgggatggcc tcgatgatga
     4381 cttccggagc gcaggatgcc ctccgatgcg gagttggact gcacgagcca acacaggttc
     4441 accgcacgcc agcgctcctc aagttgaaaa accgctggcg gcaccccaga tcgaccgcac
     4501 cgccacccga gatgtcgcgc catgttgcag atcaactgtc cccactgttt cgtgaccaac
     4561 cgcctgccgg cgcagcggtt gggtgatcgg cccaactgcg gtcgctgcaa gcagccgctc
     4621 ttcaccggca caccgctcga actgaccgaa gccaacctga ccgcgacatt gacgcgcaac
     4681 gacattccgc tggtcgtcga ctgctgggcc gcctggtgcg gtccctgccg cagcttcgcc
     4741 ccgacgttca gtcaggcggc agcccgctat gagccccacg cccgcttcgc caagctcgac
     4801 accgaggccc ttcctgcggt ggcccagcag tgggcgatcc gcagcatccc gaccctgatc
     4861 ctgttccgca acggtcagga ggttcagcgc caggccggcg ccctgtcacc accgcaactg
     4921 gcccagtggc tgaagcaggc gggggtggtt tagaaaagcc tgcggcgact ttggatcagg
     4981 aacaggccga ctccgtcggt gttactggag gtattgcttg gcaggagaga agcaagcgtc
     5041 agaaggctga ttcttcgccc cggtcaatgc gttggagaat ggtggtcgcg agcgcccgaa
     5101 aatcgttccc tgcgtcgcgg acggcttgcg aatggctgcc gattgcgcca tccgccgagg
     5161 tgagatggaa cacgggcttg cgcgtctcct gggccatggg gatcaggctg cggtagtgct
     5221 tgagtcgagc caggcaattg ccgtcgtcag cgtgggcggg tgtcggagat tgcacacccg
     5281 tgacggtttc aacatacgta gctggaatcc ggtcgaccca ttttttgtaa gccttcactg
     5341 gacgtgacag gcgctccatg tgctgcatca ggatgtaacc cagtggcgcc atgcggccag
     5401 ccggcagcaa aaactccggc tccggccagt tgttcagccg cttttgccag tcggaccgcc
     5461 aagcggtcaa tgtggggccg aggttgcgca ggccctgcag cgagaacaga tcggccgcta
     5521 gcggaatgac cacatgatcg ctgccgatca gggccgaacg gttgatggcg cccaggttcg
     5581 gcccgacgtc ggccaggatc agatcggctt cgtgttcggc agcggccatt tgtgccacct
     5641 gccaaaaggc ggtgagcagg cggaagggcc gatagaggtt gttcgaaccc atggcctcaa
     5701 gccacgcctt ggacagctcg tcctcgaacc cggccaatgc cagatcgccg ggcaccagca
     5761 ccagcccggg atggatggac tgcaccaccg gcggacggat gtcgccgacc ttggtgagcg
     5821 gctgcacgca gcggaagatc gtcgtgccgg actcgactgg agccggtggg tcccacaggg
     5881 tttccagcgt ctcttctgcg agaaaggccg atgtcagatt ggcctggggg tcgagatcga
     5941 tggcgaccac gcgcttgccc agttcgcaga gcatccaggc aacgtggaaa accagcgagg
     6001 tcttgccgac gccgcccttg ttgttgaaaa aggtgagaac cggggtgctc atggcgccct
     6061 gcgaagggtt gccagaacca tggtcggttc cacgcggaac tcgggtggtg gattgccatt
     6121 ggctgcgagc gcgcggcgtg cctgggcgat accgaagccg aaacgctgta cgaagccaag
     6181 taccttgagg acgccagcca aaatctggtt gcggtaatcg caggcgcccg gttggccgaa
     6241 attcgccgcg ttgacgacac catacggccc gcccgggctg tgaatctcga tgcggttgtc
     6301 gaaccagtac aggcgcaccg gggcgttggt gccttcgtag gtgcggtgca tcaccgcgtt
     6361 gcgcagcagc tgctgcaggg cgctcatcgg gtaaggcgtg cgccgctcct cgatcgtggc
     6421 ggaggtgaaa tcgacttcag tggtcagaaa ggccgcgagc ttctcgtccg ccctgcgcag
     6481 catctgctgc agggtcccat ccaattctgc ggcgtcggcg atcgggtcgg ccagttcggt
     6541 gccgcgtatg cgcaggaact ggatgtacgc gcacggcacc caggtgcgcg gcgatttccc
     6601 cagtgtgaga atgccggcga ccgtgggcgt cgggtcggcc gccgacgcga tcatgcccag
     6661 agatgccaga cgatcttcgt aactgcggtc gttggcggca agcacgtcag gggctatcgc
     6721 ctgcggcagg aactcacgtt caaacgtcag tcgatccagc tcatcgagcg ggcagccgta
     6781 cacaggcgtg gcgtcgaatg cccggtccag atggcggcgc ctctcattca ggatccgctc
     6841 gtcctgcgcg gaggccaagc cgcgccttgg gccagttcgg atccagatgc ggccatccag
     6901 ccggaccggt ggcgcatccg ccggccacac tgtgaccacg gccacgtcgt ggcctttgag
     6961 aaaccgcctg gtgaccgtca gcgtgggtgg cggcgtgatt tttccgtcgg tcttgatgtc
     7021 cgccagggtt tgcagaagcg cgtcggtgac cacgacctcc gggccagccg gcgtgccgtc
     7081 gtcggcgata ccgaccagca ggacaccggg tttgcggtga ttgggcaagt cgttggcgaa
     7141 agcgcagacc gcctggcgcc ctttttccgg tgcatcgccc ttccaggcac gcttgcgctc
     7201 gacccgatcg ctttcgaggt cgtcggccaa cgcggccagc tcgatgtcgg tcaggggagt
     7261 catgtggcgt tctcctggca ttcgcgcaag gccgtcgtca gtgcggccga ggcgataaga
     7321 gaatggtcgg gacaactgat tgggtcacac aaaatagagt aatccatccg cttaaccccc
     7381 acccaccatg aaggccgcca gccgccggta gaactcgacc agcatcgcgg cggtggcgcc
//
LOCUS       DAORCQ010000109         7408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_7105_length_7408_cov_5.197334, whole genome shotgun sequence.
ACCESSION   DAORCQ010000109 DAORCQ010000000
VERSION     DAORCQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7105_length_7408_cov_5.197334"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /gene="aceB"
                     /locus_tag="PKI23_09995"
     CDS             complement(<1..1333)
                     /gene="aceB"
                     /locus_tag="PKI23_09995"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HNN37306.1"
                     /translation="MSSTGQTISGLTLKGELRPEYQQILTPEALAFVAELVEKFQPRR
                     NQLLQARVARQQQFDAGQLPDFLPETQTIRSGNWKIRGIPHDLQDRRVEITGPVDRKM
                     IINALNANVKVFMADFEDALSPTWDNAIQGQINLRDANLGVIEYTNPDNGKQYRLKEQ
                     PAVLIARVRGLHLEEKHVTFAGENLSGALFDFGLYFYHNHKIRLGNGTGVYYYIPKLQ
                     SHHEAKWWDEVFAYTEKKFGVEVGTIKATVLIETLPAVFEMDEILHEMRDHIVALNCG
                     RWDYIFSYIKTLRRHPDRVLPDRQVVTMSQPFLSAYSRLLIRTCHKRGAMAMGGMAAF
                     IPVKDPVKNDEVLRKVRADKELEASNGHDGTWVAHPGLADTAMEVFDRIIPAGQPNQL
                     NVLREQDAPISAADLLQPSEGERTEVGMRTNIRVALQYTEAWISGSGCVPIY"
     gene            1658..2569
                     /locus_tag="PKI23_10000"
     CDS             1658..2569
                     /locus_tag="PKI23_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNN37307.1"
                     /translation="MQESLLKRKKLTSAEEDAHRALEQTQRYQSGAFKLAFADREFML
                     RDELRPVRLQLELLKPEMIQNERQVYCTVVVFGSARFVSHEAALDALEQASVQARQQP
                     EDAVAQRHLRHAERRLAQAYYYEEARRFGALVTEHSINQLGISLKIVTGGGPGIMEAA
                     NRGAFEANGDSIGLNIVLPMEQAPNPYITPELCFQFHYFAIRKMHFLMRAEALVCFPG
                     GFGTLDELFETLTLIQTHKSRKVPVLLFCREFWTQLINFDLLVDEGMISADDLRLFEY
                     VETAEEAWEAITRFYHSEPLHPALPGQ"
     gene            2660..3733
                     /locus_tag="PKI23_10005"
     CDS             2660..3733
                     /locus_tag="PKI23_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNN37308.1"
                     /translation="MAALTQHPSSVAQKLQTLRGNRALRLLSDAEIRQQLAGITVPKE
                     ADAVFEGGGVKGIALAGAIQVAELLGVRWKSVAGTSAGAIAAALLAAGYSSRELVTIL
                     TREMDFNKFMDEGIVDKLPLIGKLASALFEKGIYEGDYFESWLGQKLAQRGVHTFADL
                     RDGQGGYRLKMIASDVTQRKMVVLPDQLPEYGRDPDSFPVARAARMSMSIPYFFEPVM
                     LDYPGASGARCKAYIVDGGMLSNFPVWLFDAPAGTAPRWPTYGFKLVEPDSDRPAPER
                     WPHQYIMALINTMMEAHDRAYIEQANFKRTIAIPTMGIQTTQFNLTDEEETLLVRAGQ
                     EAAIKFFREWSFDRHNQTYRGTP"
     gene            3730..4575
                     /gene="rlmJ"
                     /locus_tag="PKI23_10010"
     CDS             3730..4575
                     /gene="rlmJ"
                     /locus_tag="PKI23_10010"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNN37309.1"
                     /translation="MNYRHGFHAGNFADVMKHLCLLLLMRALQAKEKPLAVLDTHAGH
                     GCYDLASVQAQRSGEWRDGIGKLWEGGANPAVLADYLTQVRGLQPPSERAKLRWYPGS
                     PWLLRQLCRPQDRLILCELLPEAVADLRALFRHDPRVSIHQRDGFAALTALLPFAERR
                     GLILVDPPYEAEDEFERLLRSVEAGHHRFPTATFMLWYPITARNPIERFLRRVRNSTL
                     RKVLQVQLLPGHVEQGLLGSGLLIINPPWQSEQAIATAMSALATRLNDTQASASVEWL
                     VAEQR"
     gene            4623..6383
                     /gene="dauA"
                     /locus_tag="PKI23_10015"
     CDS             4623..6383
                     /gene="dauA"
                     /locus_tag="PKI23_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HNN37310.1"
                     /translation="MKNHYFATALRESLRQGYGWPALRADLLAGVTVGIVALPLAMAL
                     AIASGVPPQQGLYTVVIAGAVIALAGGSRVNISGPTAAFVVVLLPITQQFGLGGLLLA
                     SVMAGVILLAMGLGRLGRFIELVPYPVTIGFTAGIGVVIATLQLKDFLGLDVGASQGH
                     FLSQLWALLQALPSLHWQDCLIGLLTLAVLLLWPRWVKKIPGHLAALLVGSVTALLLT
                     RWLPGFEVATIGSRFQYHIGGVIGHGIPPLLPHFDWPWRFSGADGKALALDFDTVQAL
                     LGPAFAIAMLGAIESLLCAVVADGMAGTRHDSNGELIGQGLGNIVAPFFGGIPATAAI
                     ARTATNVRAGAVSPLAAVIHALVVLGAMVSLADLLGYIPMAALAALLLVVAWNMSEAR
                     HFVRVIRIAPGNDILVLLTCFGLTVLFDMVIAVTVGIGLAALLFIKRSIDLTESHRVE
                     RPALSAEEMPEQIALYDINGPLFFGAAHKALGTLKTVTPGLRVVLLDLRDVNLVDMTG
                     LVALESLIETARRQQLGLVFCGLNARLKVKLRRIGIRKQRGHLEFANNIDEGIERALR
                     MRDAQPAPSGDSSTAWRPDD"
     gene            6561..>7408
                     /locus_tag="PKI23_10020"
     CDS             6561..>7408
                     /locus_tag="PKI23_10020"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNN37311.1"
                     /translation="MLCAAGAAADHRPPLRFGSNLWVGYEPFYLARQQGLYAAHEAHA
                     LRLIQLSSASDVLHAFRSGRLEVAAITLDEALRLADEGTPFRTVLLIDISNGADALLG
                     GPQVSTLAELNGKRVGVEDGATGAILLHHALNKAGLTPAQLRIVPLKFDQHRQALQQG
                     QVDALVTFDPVRSQLLAGGAHLLFDSSEAASSIVDLLIVTPEVLKSAEQRAALTALIR
                     GYFSMLAQLRGGNANLLAQAAANLELTPEQLQQAMQLIEQPDRVRNCALLCGTAPPFE
                     ATVRQV"
BASE COUNT         1313 a         2367 c         2320 g         1408 t
ORIGIN      
        1 cgtagatcgg cacgcagcca ctgcccgaga tccaggcctc ggtgtattgc agtgccacac
       61 ggatgttggt gcgcatgccg acctcggtgc gttcgccctc ggagggttgc agcagatcgg
      121 ccgcgctgat cggcgcatcc tgttcgcgca gcacattgag ctggttgggc tgtccggccg
      181 gaatgatgcg gtcgaacacc tccatcgccg tgtcggccag acccgggtgg gccacccagg
      241 tgccatcatg gccattgctg gcttcgagct ccttgtcggc gcgcaccttg cgcagcacct
      301 cgtcgttctt caccggatcc ttgaccggaa tgaaggccgc catgccaccc atcgccatcg
      361 caccacgctt gtggcaggtc ctgatcagta accgcgaata ggcgctcagg aagggctggc
      421 tcatcgtcac cacctgacgg tccggcagaa cgcgatcagg gtggcgccgc aatgtcttga
      481 tatagctaaa aatatagtcc cagcgtccgc agttgagcgc aacgatgtgg tcgcgcatct
      541 cgtgcagaat ctcatccatc tcgaacacgg ccggcagggt ctcgatcagc accgttgcct
      601 tgatggtgcc gacctcgaca ccaaacttct tctcggtgta ggcgaagacc tcatcccacc
      661 acttggcctc atggtggctt tgcagcttgg gaatgtagta gtagacccct gtgccgttgc
      721 cgagcctgat cttgtggttg tgatagaaat agagcccgaa atcgaacagc gcaccggaga
      781 ggttctcgcc ggcgaaggtg acatgcttct cttccagatg caggccacgc acgcgggcga
      841 tcagcaccgc cggttgctct ttcagccgat actgcttgcc gttgtccggg ttggtgtatt
      901 cgatgacgcc gaggttggcg tcacgcaggt tgatctgccc ctggatggcg ttgtcccagg
      961 tcggggagag cgcatcctcg aaatcggcca tgaacacctt cacattggcg ttcagcgcat
     1021 tgatgatcat cttgcgatcg accggaccgg tgatctcgac ccggcggtcc tgcagatcgt
     1081 gcggaatgcc gcggatcttc cagtttccgc tgcggatcgt ttgggtctcg ggcagaaaat
     1141 cgggcagttg cccggcatcg aactgctgct gacgggcaac ccgcgcctgc agcagctgat
     1201 tgcgacgcgg ctggaacttc tcgaccagct cggcgacgaa agcgagggcc tcgggggtca
     1261 gaatctgctg atattccggg cgcaactcac ccttcagggt cagtccgctg atggtttggc
     1321 ctgttgaaga catggtggta cctcgatgcc agttcttgac tgcgcttgcc agtttttcgg
     1381 caaaaaaaca gtcggttgaa gccagcctca cgctgccttg tgggcaaccg cgacgataac
     1441 gaggcaaatt caaagagatg gcgcttgaat cggcccggca ctgatcagat tggttcctgt
     1501 ttggggggct gaattttctt gttttttgtt gatgccgtca agcaaacccg aacagccggt
     1561 ctactgaacc aaagccctgg ccgcaggcag ggatcacccg cagggggcat aatgaagcca
     1621 gaccatttgg gtgtgaattt ttatctggag agtcaagatg caagagagcc tgctgaagag
     1681 aaagaaactg acgtcggccg aggaggatgc gcaccgggca ctggagcaga cgcaacgcta
     1741 ccagagcggc gccttcaaac tcgcctttgc cgaccgtgaa ttcatgctgc gcgatgaact
     1801 gcggccggtg cgattgcagc tcgagttgct gaaaccggag atgatccaga acgagcgcca
     1861 ggtctactgc accgtggtgg tgttcggcag tgcccgcttc gtcagtcatg aagcggcgct
     1921 ggatgccttg gagcaggcct ctgttcaggc acggcagcag ccggaggatg ccgtggcaca
     1981 acgccacctg cgtcatgccg agcggcggtt ggctcaggcc tactactacg aagaggcgcg
     2041 ccggtttggc gccctggtca cagaacattc gatcaatcag cttggcattt cgctcaagat
     2101 cgtcactggc ggcggtcccg gcatcatgga agccgccaat cgcggcgcct tcgaggccaa
     2161 tggcgacagc atcggcctca acatcgtgct gccgatggag caggctccca acccttacat
     2221 cacgccggaa ctctgctttc agtttcacta cttcgccatc cgcaagatgc actttctgat
     2281 gcgtgccgag gcgctggtct gctttcccgg gggctttggc acgctggacg agctgttcga
     2341 gaccctgacg ctgatccaga cccacaagag ccgcaaggtg ccggtgctgc tgttctgccg
     2401 tgaattctgg acccagttga tcaacttcga cctgctggtg gatgagggga tgatctccgc
     2461 ggatgatttg cggctgtttg aatatgtcga aaccgccgag gaggcctggg aggcgatcac
     2521 ccgtttctac cacagcgagc cactgcatcc ggcattgccg ggccaatgag cccacgtccg
     2581 agtcggttgc tcagcctgca tagagggcgt cacaatgtcc tggcccgaca atcgatgcct
     2641 caaccgggag cagaacagca tggccgcatt gacccaacac ccttcatccg tggcgcaaaa
     2701 gctacagaca ctgcgcggca acagggccct gcgcctgctg agcgatgccg aaatccgcca
     2761 gcagctcgcc ggcatcaccg tgccgaaaga ggccgatgcg gtgttcgaag gcggtggcgt
     2821 caagggcatt gcgctggctg gcgcgatcca ggtggccgaa ctgctcggcg tgcgctggaa
     2881 gagcgtggcc ggcaccagtg ccggcgccat cgccgcagca ctgctggcgg ccggttacag
     2941 cagccgtgag ctggtcacca tcctcacccg cgagatggat ttcaacaaat tcatggatga
     3001 ggggatcgtc gacaagctgc cgctgatcgg caagctggcc tcggcgctgt tcgagaaggg
     3061 catctacgaa ggcgactatt tcgaatcctg gctgggccag aaactggcgc agcggggcgt
     3121 gcacaccttt gccgatctgc gcgatggcca gggcggctat cggctgaaga tgatcgcctc
     3181 ggacgtgacc cagcgcaaaa tggtggtgct gcccgatcaa ctgcccgagt atggccgcga
     3241 ccccgacagc tttccggtcg cacgggccgc gcgcatgagc atgagcatcc cctacttctt
     3301 cgagccggtc atgctcgact accccggcgc cagcggcgca cgctgcaagg cctacatcgt
     3361 cgatggcggc atgttgtcga actttccggt ctggctgttc gacgcccccg ccggcaccgc
     3421 gccgcgctgg cccacctatg gcttcaagct ggtggagccc gactccgacc gccccgcacc
     3481 agagcgctgg ccgcatcaat acatcatggc gctgatcaac acgatgatgg aggcgcacga
     3541 ccgcgcctac atcgagcagg ccaacttcaa gcgcaccatc gcgattccga ccatgggcat
     3601 ccagaccacc cagttcaacc tgaccgacga agaggagacg ctgctggtcc gcgctggtca
     3661 agaggccgcc atcaagttct tccgggagtg gagcttcgac cggcacaacc agacctaccg
     3721 cggcactcca tgaactaccg ccacggcttt catgccggca attttgccga cgtgatgaag
     3781 cacctctgtc tgctgctgct gatgcgggcg ctgcaagcga aagagaaacc gctcgccgtg
     3841 ctcgacaccc atgccggcca tggctgctac gacctcgcca gcgtgcaggc acagcgcagc
     3901 ggcgagtggc gcgatggcat cggcaagctg tgggagggcg gggccaaccc ggccgtactg
     3961 gccgactatc tgacacaggt gcgcggtctg caaccgccct ccgaacgcgc caagctgcgc
     4021 tggtaccccg gctcgccctg gctgctgcgg cagctctgcc gcccccagga tcggctgatc
     4081 ctctgcgaac tgctgccgga ggcagtggcc gatctgcgtg cgctgtttcg gcacgatcca
     4141 cgggtatcga tccaccagcg cgatggtttt gccgcactca ccgccctgct gcccttcgcc
     4201 gagcggcgcg gactgatcct ggtcgacccg ccctatgaag cggaggatga gttcgagcgg
     4261 ctgctgcgca gtgtcgaggc tggccatcac cgctttccca ccgcgacctt catgctctgg
     4321 tacccgatca cggcccgcaa cccgatcgag cgctttctgc gccgggtgcg caacagcacg
     4381 ctgcgcaagg tgttgcaggt gcaactgctg ccgggccatg tggagcaagg gctgctcggc
     4441 agcgggctgc tgatcatcaa tccaccctgg cagagcgaac aggccatcgc caccgcaatg
     4501 tcggcgctgg cgacccggct gaacgacaca caggcgagtg ccagcgtcga atggctggtg
     4561 gcagaacagc gctgagcacc ccaaagcctg ttcgaagcag atcgagacga ggagacaact
     4621 caatgaaaaa tcactatttc gccaccgcgc tgcgcgaaag cctgcgtcag ggctatggct
     4681 ggcctgctct gcgcgccgac ctgctggccg gcgtgacggt cggcatcgtc gcgctgccgc
     4741 tggcgatggc actggcgatc gccagcggcg tgccgccgca gcagggtctc tacaccgtgg
     4801 tcatcgccgg cgcggtgatc gcactcgccg gcggttcgcg ggtcaacatc tccggtccca
     4861 ccgccgcctt cgtcgtggtg ctgctgccga tcacacagca gttcggcctc ggcggcctgc
     4921 tgctggcctc ggtgatggcc ggtgtcattc tgctggcgat ggggctgggc cggctcgggc
     4981 gcttcatcga gctggtcccc tacccggtca ccatcggctt caccgccggc atcggcgtcg
     5041 tcatcgccac cttgcagttg aaggattttc tcggcctcga tgtgggcgcg agtcaggggc
     5101 actttctgtc ccagctgtgg gcgctgctgc aggcgttgcc gagcttgcac tggcaggact
     5161 gcctgatcgg cctgctgacg ctggctgtgc tgttgctgtg gccgcgctgg gtcaagaaaa
     5221 ttcccggcca tctggcggcg ctgctggtcg gcagtgtcac cgccctgctg ctgacgcgct
     5281 ggctgcccgg cttcgaggtg gccaccatcg gctcccgctt ccagtaccac atcggcggcg
     5341 tcatcggcca cggcatcccg ccgctgctgc cgcactttga ctggccatgg cgcttcagcg
     5401 gcgccgatgg caaggcgctg gcgctcgact tcgacaccgt tcaggcgttg ctgggaccgg
     5461 cctttgccat cgccatgctg ggggcgatcg agtcattgct ctgcgccgtg gtggccgacg
     5521 gcatggcggg cacccgccac gactccaatg gcgaactgat cggccagggc ctcggcaaca
     5581 tcgtcgcgcc tttttttggc ggcattccgg ccactgcggc gattgcccgc accgccacca
     5641 atgtccgcgc cggcgcggtg tcgccgctcg ccgccgtgat ccatgcgctg gtggtgctgg
     5701 gggcgatggt cagtctggcc gatctgctcg gctatatccc gatggcggcg ctggcggcat
     5761 tgctgctggt ggtggcctgg aacatgagcg aggcgcgcca tttcgtccgc gtcatccgca
     5821 tcgcgcccgg caacgatatc ctggtgctgc tgacctgttt cggcctcacc gtgctgttcg
     5881 acatggtgat tgccgtcacc gtcggcatcg ggctggccgc gctgctgttc atcaagcgca
     5941 gcatcgacct gaccgaaagc catcgcgtcg agcgcccggc gctgtcggcg gaggagatgc
     6001 ccgaacagat cgccctctac gacatcaatg gcccgctgtt cttcggtgcc gcacacaagg
     6061 cgctgggcac gctgaagacg gtcacgccgg ggctgcgggt ggtgttgctc gacctgcgcg
     6121 atgtcaatct ggtggacatg accgggctgg tggcgctcga atcgctgatc gaaaccgccc
     6181 ggcgccaaca gctcgggctg gtgttctgcg gtctcaatgc gcggctgaag gtcaagctgc
     6241 gtcgcatcgg cattcgcaaa cagcgcggcc atctggagtt tgccaacaac atcgatgaag
     6301 gcatcgagcg cgcattgcgg atgcgcgatg cgcagccagc cccatccggt gacagctcga
     6361 cggcatggcg acccgatgat tgaacagcta tagttttcag tgccaaaccg ccattccgtt
     6421 ctgcaacaac ccgttggaac cgcccagtgc agcgcgagaa tgatcgagaa aacgataaat
     6481 tgaacagcgc agcacccccg cctttgtggc gcccgccccg ccttcaaacc ctcaacagcc
     6541 tgctgctcat ggcgtggctg atgctgtgtg cggctggcgc agcggccgac caccgcccgc
     6601 cactgcgatt cggcagcaac ttatgggttg gctatgaacc cttctatctc gctcggcaac
     6661 agggacttta tgccgcgcac gaggcccatg cactgcggct gatccagttg tcttcagcca
     6721 gcgatgtgct gcatgccttt cgcagcggcc gtctcgaggt cgcggcaatc acgctggatg
     6781 aagcattgcg gctggcggat gaaggcactc ccttcaggac cgtattgctg atcgacatct
     6841 cgaacggtgc cgatgcgctg ctcggtggtc cgcaggtcag cactctggcc gaactgaatg
     6901 gcaaacgggt cggcgtcgaa gatggtgcga ccggcgccat cctgctgcac cacgcactga
     6961 acaaggctgg gttgacaccg gctcaactgc gaatcgtgcc attgaaattc gaccagcatc
     7021 ggcaggcact gcagcagggg caggtcgacg cgctggtcac ctttgacccg gtgcgctcac
     7081 aactgctggc cggcggcgcc cacctgctgt tcgacagcag tgaggccgcc agctccattg
     7141 tcgatctgct gatcgtcaca cccgaagtgc tgaagagtgc ggagcagcgc gccgcattga
     7201 cggctctgat ccggggctat ttttcgatgc ttgcccaact gcgtggtggc aacgccaatc
     7261 tgcttgcaca ggccgcagcc aacctggagt tgacgcccga gcagctccag caggccatgc
     7321 agctgatcga acagcccgat cgggtgcgca actgcgcatt gctgtgcggc actgcaccgc
     7381 ccttcgaggc gacggtgcgc caggtgag
//
LOCUS       DAORCQ010000110         7167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_7417_length_7167_cov_5.401715, whole genome shotgun sequence.
ACCESSION   DAORCQ010000110 DAORCQ010000000
VERSION     DAORCQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7417_length_7167_cov_5.401715"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1835)
                     /locus_tag="PKI23_10025"
     CDS             complement(111..1835)
                     /locus_tag="PKI23_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNN37312.1"
                     /translation="MPAEVIIHTTFGEIATLLALAAGIGMLGAALRQPLIVSFIAVGL
                     IAGPSGLDVVHSDAQIDLLAELGIAMLLFLVGIKLDTKLMRSLGPVALLTGLGQVIFT
                     LGLGYLIGLGLGFDPITSLYVALALAFSSTIIIVKLLSDKREIDALHGQIALGFLIVQ
                     DLVVIAAMIAVSAIGIGAVEGHGGGDVISVLLSGLALVALVALFVRHVATPLTEWLAH
                     APELLVIFAIAQTAIFAAIGDFVGLGKEVGGLLAGISLASTPYRETMAARLAPLRDFL
                     LLFFFISLGARLDLSLLGAHLTEALLFSLFVLLGKPLIVLIIMGAMGYRKRTGALAGI
                     AMAQISEFSLIFVAMGVTLGHIEQETFGLVTLVGLVTIALSTYMITYSHQLYAWCEPL
                     LAPFERLETIRERTQPASQLHNPPSVIIFGLGRFGAAIAMRLHRHGLPVLGIDFNPQA
                     VRRCNGFGIDTRYGDATDSELIADLPLAEAQWLVSTTPNHAAGLTHDDTRIILIQLAR
                     STGFTGRIAVTSHSGDESEALLAMGADLVIEPFQDAADRAVELLSGARLEERIEIPPI
                     ETEQQPIP"
     gene            2012..2662
                     /locus_tag="PKI23_10030"
     CDS             2012..2662
                     /locus_tag="PKI23_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37313.1"
                     /translation="MCFSASASFLVGASLLVVGATTLNKVSRRVELPFASIPLLFGVQ
                     QLMEGGIWLGLQFDAPTATQLLTLLYSLFSHLLWPIYVPLAMLLLEPEPARRKINLAF
                     VWAGVAAALYLLYVLIRFPLQAKLVGGHILYGSFHDYALLPMGAYLVATCVSLLFSTH
                     RMVEVLGFVLLLSCIAAYLFYRLWFISVWCFFAAILSAMVYLYFRRRVARTAQGLG"
     gene            complement(2697..3491)
                     /locus_tag="PKI23_10035"
     CDS             complement(2697..3491)
                     /locus_tag="PKI23_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN37314.1"
                     /translation="MELGLTAKQALVTGSTAGIGFATARSLLREGAQVTLNGRTQARV
                     DAALAQLRAEFPAASVSGIAADLSSAEGCQRLIEQLPEVDLLVNNLGIFEPKPFEQIG
                     DEDWLRFFQTNVMSGVRLARHYVGGMRARNWGRIIFVSSESALQIPTETIHYGMTKTA
                     QLAISRGLAETLTGTGVTVNSVLPGPTASEGVTRFIDQMIKSHGIDAATAEREFFRSA
                     RPSSVLQRFTTPDEVAAMITYLCSALASGTTGAALRVDGGVVRAIA"
     gene            complement(3569..5503)
                     /locus_tag="PKI23_10040"
     CDS             complement(3569..5503)
                     /locus_tag="PKI23_10040"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNN37315.1"
                     /translation="MAKMSFNEWPAITADDATIKAALEEANIPALMAALAHLTGSTAI
                     LRGDIQPNNNIFRDPQSGIAPEQQAKARAMAFEIITAYRDAGSRLPPVPDENSVREMM
                     HYLIGQEISPDYVQFLMSELALNGEDPYSQAGINALPQRLKSQFKVAVIGAGMSGILA
                     GIRLKEAGIPYVIFEKNGDVGGTWYENTYPGCRVDSPNHSYSYSFAPNDWPQYFSSQA
                     VLRDYFDRCATEYGIRDAIHFNTEVVSAEFDETSKIWRLHLNSRDAGPRTLEFNAIIS
                     AVGQLNRPKFPDIEGRDRFKGIAFHSAQWQHQHDLRGKRIAVIGTGASAFQFVPEIAR
                     TASEVLIFQRTPPWLSPRDNYHDHIPEGKHWLLNNLPFYTKWFRFLMFWQTSEGLLKA
                     VKCDPDWHGHPNSVSAEHDQLRQMLTHYLTQMVGDDPALLAKVIPDYPPAGKRMCVDN
                     GTWIRALKRDNVQLFTDGIGEITETGLTTRDGQHRAVDVIIYGTGFKASRLLWPMQII
                     GRGGVELQQQWNDDPHAYLGITVPGYPNLFCLYGPNTNIVVNGSIIFFSECEMRYIML
                     ALQQLLERNQRTIECKQSVHDAYNDWIDAGNQQMAWGSPRVQSWYKNSQGRVTQNWPY
                     TLLEFWRRTRTLNPDDYQFG"
     gene            5768..7159
                     /gene="sthA"
                     /locus_tag="PKI23_10045"
     CDS             5768..7159
                     /gene="sthA"
                     /locus_tag="PKI23_10045"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019096213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNN37316.1"
                     /translation="MAREFDVVVIGTGPAGEGAAINAAKHGKRVAVVEDRGAVGGACT
                     HLGTIPSKALRHVVKQISEFNSNPLFRDVGEPRWFSFPQVLKRAESVIAKQVQLRTQF
                     YSRNRISLFSGHAKFVDAHTLVVQSEGRTNEVLKARHFVIATGSRPYRPTGVDFDHPR
                     IYDSDTILHLEHTPRTIIIYGAGIVGCEYASILAGLGVQVNLVDQHDEILSFLDDEIS
                     DALSYHLSNNGVLIRLNEEYERIEADSQRVYLYLKSGKKIKGDALLWCNGRSGNTVGL
                     GLEKIGLTPDTRGRIAVNGNYQTDVEHIYAAGDVIGWPSLASAAHDQGRAVANHLLAN
                     GQFHHIDDVATGIYTIPEISSIGRTERELTADRVPYEVGRAFFKSMARAQIAGEEVGM
                     LKILFHTETLEILGVHCFGDRAAEIIHIGQAIMRQDGKANSINYFVDTTFNYPTMAEA
                     YRVAALNGLNRLA"
BASE COUNT         1368 a         2133 c         2222 g         1444 t
ORIGIN      
        1 ggaaccactg gtctgcatca agggatagtc gaccgcggta tgcgcatcca gctcggtcat
       61 cggctgctcc ttggtcgctg gtcgattggg ttctcctcga tcgatcaggc tcagggtatc
      121 ggctgctgct cggtttcgat cggtggaatc tcgatgcgct cctccagcct ggcaccgctc
      181 agcagttcga ccgcgcggtc ggcagcgtcc tggaagggtt cgatcaccag atcggccccc
      241 atcgccagca gggcttcact ctcatcgccg ctgtgggagg tgaccgcgat gcgcccagtg
      301 aagccggtac tgcgtgccag ttggatcagg atgattcgcg tgtcgtcgtg ggtgaggccg
      361 gcggcatggt tcggcgtggt ggagaccagc cactgggcct cggccagcgg caggtcggca
      421 atcagttcag aatcggttgc atcaccgtag cgggtgtcga tgccaaaacc gttgcagcgt
      481 cgaaccgcct gcggattgaa gtcgatgccc agcacgggca ggccgtgccg atgcaggcgc
      541 attgcgatcg ccgcgccaaa gcgccccaga ccgaaaatga tcaccgaagg cgggttgtgc
      601 aactgactgg ccggctgcgt ccgctcacga atggtttcca gacgctcgaa gggggcgagc
      661 agcggttcgc accaggcgta gagctggtgg gagtaggtga tcatgtaggt ggagagggcg
      721 atggtcacca gcccgaccag ggtcaccaag ccgaaggtct cctgttcgat gtggccgagc
      781 gtgaccccca tggcgacgaa gatcaatgag aactcgctga tctgggccat ggcgatgccg
      841 gcaagagcgc cggtgcgttt gcgatagccc atggcgccca tgatgatgag tacgatcagt
      901 ggcttgccaa gcagcacgaa cagcgaaaag aggagcgctt cggtcagatg tgcgccgagc
      961 agcgagaggt cgagcctggc gcccagtgag atgaagaaga acagcagcag aaaatcgcgc
     1021 agcggtgcca gacgtgccgc catggtttct cgataggggg tcgaggccaa tgagatgcct
     1081 gccagcaggc cacccacctc cttgccgagc ccgacgaaat cgccgatcgc cgcgaagatc
     1141 gcggtctggg cgatggcgaa gatcaccagc agttccggcg cgtgcgcgag ccactcggtc
     1201 agcggggtgg cgacatggcg cacgaacagt gccaccagtg ccaccagcgc cagcccggag
     1261 agcagcacgc tgatgacatc gcccccgcca tggccttcga cggcgccgat gccgatcgcc
     1321 gacactgcga tcatcgccgc gatcaccacc agatcctgca cgatcagaaa accgagcgcg
     1381 atctggccat gcagcgcgtc gatttcgcgt ttgtcggaga gcagcttgac gatgatgatg
     1441 gtcgacgaga acgccagcgc cagcgccaca tagagactgg tgatcggatc gaaccccagg
     1501 ccgagcccga tcagataacc caggcccagg gtgaagatca cctggcccag gccggtcagc
     1561 agggccaccg gtcccaggga gcgcatcagc ttggtgtcga gcttgatgcc gaccagaaac
     1621 agcagcatgg cgatgccaag ttcggcgagc aggtcgatct gcgcatccga atgcaccaca
     1681 tccagccccg aggggcctgc gatcagacca acggcaatga agctgacgat cagcggctgg
     1741 cgtagcgcgg cgcccagcat cccgatgccg gcggccagcg ccaacagggt ggcaatctca
     1801 ccaaatgtcg tatgaatgat gacctccgca ggcatggcat gcactggcgt cgcagtgcct
     1861 gtttctcgtc atcgaaaccc gactccgccc ttttttcttt cgaaactgca aaacagcaca
     1921 ttgcgccgcg ctgccaacaa cctgatctat tatgcactct tgcgcgagcc tgcatccggc
     1981 ctcggcgaca cccctgaatg agacgaaccc catgtgcttt tccgcctcgg caagctttct
     2041 ggtcggcgcc tcgctgctcg tggtgggtgc gaccactctg aacaaggtgt cgcgccgggt
     2101 cgaattgccc tttgcctcga tccccctgct gttcggggtg cagcaactga tggaaggtgg
     2161 catctggctc ggtctgcaat tcgatgcccc cactgcaacc cagctgctga cactgctcta
     2221 ctcgctgttc tctcatctgc tgtggccgat ctacgtgccc ctggcaatgc tgttgctgga
     2281 gcctgaaccg gcgcgccgca aaatcaatct cgcattcgtc tgggccggtg tggcggccgc
     2341 cctctacctg ctctacgtcc tgatccggtt tccgctgcag gccaagctgg tgggtggaca
     2401 tatcttgtac ggctcgtttc acgactacgc actgctcccc atgggcgcct acctggtggc
     2461 aacctgtgtc agcctgctgt tttcgaccca ccggatggtg gaagtgctcg gtttcgtgct
     2521 gctgctctcc tgcatcgccg cctatctgtt ctacaggctc tggttcatct cggtctggtg
     2581 tttctttgcc gcgatcttga gcgcgatggt ctacctctat ttccgccgcc gcgtggcccg
     2641 caccgcacag ggcttggggt agcaaccgcc gatgccggcg cagccgattt gcagcatcag
     2701 gcaatggccc gcaccacacc gccatcgact cgcagtgccg caccggtggt gcccgacgcc
     2761 aaagcactgc aaaggtaggt gatcatggcc gcgacttcat caggcgtggt gaagcgttgc
     2821 agcaccgaag aggggcgggc gctcctgaag aactcccgct cggcggtggc ggcgtcgatg
     2881 ccatggctct tgatcatctg atcgatgaaa cgggtcaccc cttcggaggc ggtgggcccc
     2941 ggcagcacac tgttgacggt gacgccagtg ccggtgaggg tctcggccaa gccgcgcgag
     3001 atcgccagtt gtgcggtctt ggtcatgccg tagtgaatgg tttcggttgg aatctgcaac
     3061 gccgattcgc tggagacgaa gatgatccgg ccccagttgc gcgctcgcat gccgccgaca
     3121 tagtgtcggg ccagacgaac gccactcatc acattggtct gaaaaaagcg cagccaatcc
     3181 tcatcgccga tctgctcgaa gggcttgggc tcgaaaatgc cgaggttgtt gaccagcagg
     3241 tcgacctcgg gcagttgctc gatcagtcgc tgacaaccct cggcactgct gaggtcggcg
     3301 gcaatgccgc tgacgctggc cgctgggaac tcggcgcgca gctgcgccag tgcagcatcg
     3361 acccgcgcct gcgtccggcc attgagggtc acctgcgcac cctcgcgcag cagtgaccgt
     3421 gcggtggcaa aaccgatgcc ggcggtcgag ccagtcacca aggcctgctt tgcagtcaat
     3481 ccaagctcca tgtcccatct cctgtctgtt gttgccatat tgccgattgc tcaggcgcgc
     3541 cggcagtcct gcaggcgcac cgttcgactc agccgaactg gtaatcatcc gggttgaggg
     3601 tgcgggtgcg gcgccagaac tccagcaggg tatagggcca gttctgcgtc acccgcccct
     3661 ggctgttctt gtaccagctc tgcaccctgg gtgaacccca ggccatctgc tggttgccgg
     3721 cgtcgatcca gtcgttgtag gcatcatgca ccgactgctt gcactcgatc gtgcgctggt
     3781 tccgttcgag cagctgctgc aacgccagca tgatgtagcg catctcgcac tcggagaaga
     3841 agatgatgct gccgttgacg acgatgttgg tgttggggcc atagaggcag aagaggttgg
     3901 ggtagcctgg caccgtgatg ccgaggtagg cgtgtggatc gtcattccac tgctgttgca
     3961 gttcgacgcc gccacgcccg atgatctgca tcggccacag caggcggctg gccttgaagc
     4021 cggtgccata aatgatgacg tccaccgcac gatgctggcc atcacgggtg gtcaggccgg
     4081 tttcggtgat ttcaccgatg ccatcggtga agagctggac attgtcgcgc ttgagcgcgc
     4141 gaatccaggt gccattgtcc acgcacatcc gcttgccggc tggtggatag tcaggaatca
     4201 ccttcgccag cagtgccggg tcatcgccga ccatctgggt gaggtaatgg gtcagcatct
     4261 gtcgcagttg atcgtgctcc gcgctgaccg aattggggtg gccatgccag tccgggtcgc
     4321 acttgaccgc cttcagcagc ccttcggagg tctgccagaa catcagaaag cgaaaccact
     4381 tggtatagaa gggcaggttg ttgagcagcc agtgctttcc ttccgggatg tgatcatggt
     4441 agttgtcgcg cggactgagc cagggtggcg tgcgttgaaa gatcagcacc tcgctggccg
     4501 tcctggcgat ttcgggcacg aactgaaagg cgctggcgcc ggtgccgatg accgcgatgc
     4561 gcttgccgcg cagatcatgc tggtgctgcc actgcgctga atggaacgcg atgcccttga
     4621 agcgatcacg gccctcgatg tcaggaaatt tggggcggtt gagctggccg actgcgctga
     4681 tgatggcgtt gaactccagc gtccgcgggc ccgcatcgcg gctgttcaga tgcagcctcc
     4741 agatcttgct ggtttcgtcg aactcggccg acaccacctc ggtgttgaag tggatggcgt
     4801 cccgaatgcc gtactcggtc gcgcagcggt cgaagtagtc ccgcagcacg gcctgcgacg
     4861 agaagtattg cggccagtcg ttgggggcaa aggagtagga gtagctgtga ttggggctgt
     4921 cgacccggca gccggggtag gtgttttcgt accaggtgcc gccgacatcg ccatttttct
     4981 cgaagatgac gtagggaatt cccgcctctt tcagtcggat gcccgccaga atgcccgaca
     5041 tgccggcgcc gatgaccgcc accttgaact ggctcttgag cctctggggc agggcattga
     5101 tgccggcctg gctgtaggga tcctcgccgt tgagtgccag ttcgctcatc agaaactgaa
     5161 catagtcggg cgagatctcc tgaccgatca ggtagtgcat catctcgcgg acactgtttt
     5221 cgtcaggaac cggcggcagc ctgctgccgg catcgcggta ggcggtgatg atctcgaagg
     5281 ccatcgctcg cgccttcgcc tgctgttcgg gcgcaatgcc gctttgcgga tcacgaaaga
     5341 tgttgttgtt gggttgaatg tcgccgcgca ggatggcggt gctgccagtc agatgcgcca
     5401 gcgcggccat cagggcaggg atgttggcct cttcgagtgc cgccttgatg gtggcatcat
     5461 ccgccgtgat ggccggccat tcgttgaagc tcatcttcgc cattgcgttg tacctccggg
     5521 agttctgtga atgatcttcg actatggtaa tgaagaaggg tcatgctacc gctttttgct
     5581 tggcgctttg aatgcgcgct ccatcaagga gcgacagggc agggcgccga gatggccaat
     5641 gcgcgcacat ggaaaaaata aatatatcga tgtttaacaa ataattgaaa gacagatcaa
     5701 aagttttgca ttagaatttg atcaaggttt tgggtaatgt ttctggctgt ctgatcggga
     5761 gcgaaaaatg gcacgtgagt ttgatgtggt cgtcatagga acagggcctg ccggcgaggg
     5821 cgccgcaatc aatgccgcca agcatggcaa gcgggtggcg gtggtggaag accgcggtgc
     5881 cgtcggtggt gcctgcaccc atttgggcac cattccctcc aaggcgttgc gccatgtcgt
     5941 caagcagatc agcgagttca acagcaatcc actgttccgt gacgtcggag aaccgcgctg
     6001 gttctcgttt ccacaggtgc tgaaacgggc cgaatcggtc attgccaaac aggtgcagtt
     6061 gcgcacccag ttctacagcc gtaaccgaat ctcgctcttt tcaggccacg ctaagttcgt
     6121 cgacgcgcac accctcgtcg tgcagagcga aggacgtacc aacgaggtgc tgaaggcccg
     6181 ccatttcgtc atcgccaccg gatcacggcc ctatcggccg acgggtgtcg atttcgatca
     6241 tccgcgcatc tacgacagcg acaccatttt gcatctcgaa cacaccccga gaacgatcat
     6301 catctatggc gcgggcatcg tcggctgtga atatgcctcg attctggccg ggcttggtgt
     6361 gcaggtgaac ctggtcgatc agcatgacga aattctgtcg tttctcgatg atgaaatctc
     6421 cgatgccctg agttatcacc tcagtaacaa tggcgtgctg attcgcctga acgaagagta
     6481 tgaacggatc gaagccgact cgcagcgggt ctatctctac ctcaaatccg gcaagaagat
     6541 caaaggcgat gccttgctct ggtgcaatgg tcgcagcggc aatacggttg ggcttggatt
     6601 ggaaaagatc ggtctgacgc ccgatacccg cgggcgaatc gccgtcaacg gcaactatca
     6661 gaccgatgtg gaacacatct atgccgcagg ggatgtgatt ggttggccca gtcttgccag
     6721 cgccgcgcat gaccaaggac gcgccgtggc caaccatctg ctcgccaatg gccagtttca
     6781 ccacatcgat gacgtcgcca ccggcatcta caccattcct gaaatcagct cgattggccg
     6841 caccgagcgc gaactgaccg ctgacagggt gccttacgag gttggccgcg ccttcttcaa
     6901 gagcatggca cgcgcgcaaa ttgccggtga agaggtcggc atgctgaaga tcctgttcca
     6961 caccgagact ctggagattc tcggtgtcca ctgttttggc gaccgcgctg ccgagatcat
     7021 ccacattggt caggcgatca tgcgtcagga cggcaaggcc aacagcatca actatttcgt
     7081 cgacaccacc ttcaactatc cgacgatggc cgaggcttac cgtgtggccg ccttgaatgg
     7141 cctcaatcgg ctggcatgag ttgcaac
//
LOCUS       DAORCQ010000111         7054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_7589_length_7054_cov_6.314188, whole genome shotgun sequence.
ACCESSION   DAORCQ010000111 DAORCQ010000000
VERSION     DAORCQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7589_length_7054_cov_6.314188"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..384)
                     /locus_tag="PKI23_10050"
     CDS             complement(121..384)
                     /locus_tag="PKI23_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37317.1"
                     /translation="MTTTEHSIEHDLIATLGDLRILQIPARGAIGCPGYDIQTLTEVI
                     QPAACRWQATGPLLASLATVWARFKAGLAWRGYLLAFCRVQEK"
     gene            546..1046
                     /locus_tag="PKI23_10055"
     CDS             546..1046
                     /locus_tag="PKI23_10055"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNN37318.1"
                     /translation="MELLFLCTGNSCRSIMAEACCNARAGGRLHARSAGSQPTGFVHP
                     WALRQLTRAGIAVEGLQSKSWAHLPSRPQIIITLCDSAAGESCPAYLGPAVRAHWGLP
                     DPARVTGSEAVVDAAFEQVWQALCARIDALLTLPIERWLADHPAALRAELERIGALAL
                     PDVGAH"
     gene            1046..2095
                     /gene="arsB"
                     /locus_tag="PKI23_10060"
     CDS             1046..2095
                     /gene="arsB"
                     /locus_tag="PKI23_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNN37319.1"
                     /translation="MQRGEPAGLTLFERYLTLWVALCILLGIGAGELFPGLFCLIGQI
                     KLAEVNLLVGLLIWVMIIPMLLKVDFGALRQLRRHLRGIGVTLTVNWLVKPLSMALLG
                     WLFIRHLFAPWLPTAQIDGYIAGLILLAAAPCTAMVFVWSRLVEGDPLFTLSQVALND
                     SIMLFAFAPIVALLLGVSAITVPWDTLLLCLLLYVFIPLGLAQLWRRRLLARGAASLD
                     RAAASVAPWSIVALLLTLLLLFGFQGAAILGQPLIILLLAVPILLQVLFNAGLAYGLN
                     RWLGEQHCVAGPSALIGASNFFELAVATAISLFGFESGAALATVVGVLVEVPVMLLVV
                     RLVNRSRAWYGPASA"
     gene            complement(2087..2941)
                     /locus_tag="PKI23_10065"
     CDS             complement(2087..2941)
                     /locus_tag="PKI23_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37320.1"
                     /translation="MVGRILLLMVLLPLAVWSTAEERLEIIPLRHRTAEQLLPQLTPF
                     VEPTGVINGRGDTLYLRASSGNRAEIMRLIENLDRPARQLLISVRHGDSAQRHGQGGE
                     LSGSVGGSGVRIIRSGSTLPKSGTVEINRGNSQIEASGFQASTRSEEEIDQRVQVMEG
                     GRAFIQVGQSIPLPMRQVILRPDGAVVSETTQWHDIGTGFYAAPQLAGDRVTLDISPL
                     HQRAGDQPGATETREISTTVSGRLGEWINLGGSDQRSSEGRDGSGYRATEGGSDSFGI
                     WLKVDELR"
     gene            3057..3848
                     /locus_tag="PKI23_10070"
     CDS             3057..3848
                     /locus_tag="PKI23_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37321.1"
                     /translation="MPRPTGLNRSSHWPLAGMVWLLLTLGGCATVVPPTGAPATALPS
                     PTPPPSPAAPAPERARGWWYACSQFNWSSDSEPDWGLDLFVADRVVRPLIAAHRDQLP
                     LWRVHRRAAPDGAGHRFCLIFYATPQVAGQINQEWNTNPQLQRLQEGGQLRAIQLDDP
                     TRNSRPALADASDPAWPAVIGNAWPYYIMGASQMWLELITQMRSERGSPDTTELAQQL
                     EQYRQLQGQISQLWNDSGQHAFLHHLNALFGYQPVLIREQRSYRF"
     gene            complement(3876..5213)
                     /locus_tag="PKI23_10075"
     CDS             complement(3876..5213)
                     /locus_tag="PKI23_10075"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNN37322.1"
                     /translation="MLDLLILNGRMVNEGVIGETDLAIRDGRIEAMGGDLQHRPARTT
                     LDASGLLLLPGLIDDQVHFREPGLTHKGDMASESRAAVAGGITSFMEMPNCQPPTTSH
                     QALLEKHRLAASKSVANYAFYFGASNQNLAEILALDTRLACGIKVFMGASTGDMLVDD
                     PKTLEQIFANSPLLIATHCEDTPTILANEARFRQQHGEQIPIECHPLIRSEEACYRSS
                     SLAVELAKRHGTRLHVLHLTTARELALFSAGPLADKRITAEVCVHHLFFNDGDYRNKG
                     TLIKCNPAIKQESDRLALLQAVRDDRIDIIATDHAPHLLDEKARPYAQAPSGLPLVQD
                     ALLVLLELVQQGQLTLEQVVAKSSHRVAELFAIRDRGYLREGYHADLVLVDPLTPTEV
                     TRTKVLSKCGWSPFEGHRFAHSIHTTVVNGQILYRRGQPVADITALPLQFRRD"
     gene            5281..5748
                     /gene="hisI"
                     /locus_tag="PKI23_10080"
     CDS             5281..5748
                     /gene="hisI"
                     /locus_tag="PKI23_10080"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNN37323.1"
                     /translation="MSRSSLFKQLEVAPRASQVAVGDFLAALNFDQAGLIPCITQQHD
                     SGEVLMLAWMNRASLEESLASGRLCYWSRSRQRLWRKGESSGQFQWLRELRVDCDGDA
                     LLALVEQHGVACHTGRQGCFYLRLSAQGAEVMRDPAVPVEQLYGAAKAQQESE"
     gene            complement(5783..>7054)
                     /locus_tag="PKI23_10085"
     CDS             complement(5783..>7054)
                     /locus_tag="PKI23_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNN37324.1"
                     /translation="VTMRRLLATAHALLLLVLTLPAAAEQRFNLCLDSLRQQASSQGI
                     HGATLDLAFADISQRPEVLQKDRGGQSEFIYTFWHYLGRSVSPERVETGRNQIATHRE
                     LLEQISRQYGVPYHYLVALWGLETNFGRYLGDIPTLDALATLACDQRRSSMFGRQFVE
                     ALRLVDQGKIARESMRGSWAGAMGQVQFMPGTYALYAVDYDGNGRTDLWNDLPDVFAS
                     AANYLNRIGWKEQDRWGREVRVPRDLPWNLTGIKGWRPMSEWRRLGVRQVDGSPLPED
                     GRQAALLTPEGYEGPCFLVYDNFKVVMRWNASSYYALAVGHLADRLAGGPELHARPPV
                     QTFYRRAEAVEIQQRLTILGYELGQQDGLLGGRTQTAIKQFQSLHGLPVDGYPSQPLL
                     EQLRRLTPSLAVPVNAAIHSHGGKVATTVGE"
BASE COUNT         1079 a         2274 c         2409 g         1292 t
ORIGIN      
        1 gtcgatcagc cacgcaacct ggccaaatcg gtcaccgtcg aatagcgccg accgaaccgg
       61 cgaacagggc aggcatggcc ggggtcacat gtgaccctgt ttttctcatc tttgcgtgac
      121 tcacttttct tggacacggc aaaacgccag cagatatccc cgccacgcca gccccgcctt
      181 gaatcgggcc cacacggtgg caagcgaagc cagtaggggg cccgtggctt gccagcgaca
      241 cgccgccggt tgaatcactt cggtcagcgt ctggatgtcg tatcccggac aacctatagc
      301 cccccgcgca ggtatctgta aaatcctgag gtcgccgagc gtggcaatca ggtcgtgttc
      361 gattgagtgt tcggtggtgg tcatgaatca aagctcaaga tgccgaaacc ccatgccggc
      421 tggtttgcag cgtgaccaaa gccggccgga gttcaagcgg gcaggccctg gtttgccatg
      481 aaagtgtcac cgggttcggt tactctggcg gtccgattcc attcggacca cccatgcacc
      541 ctccgatcga actgctgttt ctctgcaccg gcaactcctg ccggtcgatc atggccgagg
      601 cctgctgcaa tgcccgcgcc ggcgggcggc tgcatgcccg cagcgccggc agtcagccga
      661 ccggttttgt tcacccatgg gcattgcggc aactgacgcg ggccggcatt gcggtcgagg
      721 ggttgcagag caagtcgtgg gcgcacctgc cgagtcggcc gcagatcatc atcacgctgt
      781 gcgattcggc ggccggcgag agctgtccgg cctatctggg accggcagtc cgcgcccact
      841 gggggctgcc cgatccggcc cgggtcactg gcagcgaggc cgtcgtcgat gccgcctttg
      901 agcaggtgtg gcaggcgctc tgcgcgcgca tcgatgcgct gctgacgctg ccgatcgagc
      961 ggtggctggc cgatcatccg gcggcgctga gggcagaact ggagcgcatc ggtgccctgg
     1021 cgctgccgga tgtcggagca cactgatgca gcgcggtgag ccggccgggt tgacgctgtt
     1081 cgagcgctat ctgacgctgt gggtggcgct ctgcattctg ctcggcattg gtgccggcga
     1141 gctgttcccc gggctgttct gcctgattgg gcagatcaag ctggccgagg tcaatctgct
     1201 ggtcggattg ctgatctggg tgatgatcat tccgatgctg ctgaaggtcg atttcggcgc
     1261 gctgcgccaa ctgcggcgcc atctgcgcgg catcggtgtc accctgacgg tgaactggct
     1321 ggtcaagccg ctgtcgatgg cgctgctggg ctggctgttc atccgccacc tgttcgcccc
     1381 ctggctgccg acggcgcaga tcgacggcta cattgccggc ctgattctgc tggcggcggc
     1441 cccctgcacc gcgatggtct tcgtctggag tcgattggtc gaaggcgatc cgctgttcac
     1501 gctgtcgcag gtggcgctca acgacagcat catgctgttc gccttcgcgc cgatcgtcgc
     1561 actgctgctc ggcgtttcgg ccatcaccgt gccctgggac acgttgctgc tctgcctgct
     1621 gctctatgtc ttcattccgc tggggctggc acagctctgg cgtcgccggc tgctggcgcg
     1681 gggggcggca tcgcttgatc gggccgcggc cagcgtcgcg ccctggtcga tcgtggcgct
     1741 gctgctgacg ctgctgttgc tgttcggctt tcagggggcg gcgattctcg gccagccgtt
     1801 gatcatcctg ctgctggcgg tgccgattct gctgcaggtg ctgttcaatg cagggctggc
     1861 ctatggcctc aaccgctggc tgggggagca gcactgcgtg gccggccctt cagcgctgat
     1921 cggtgcttcc aacttctttg aactggcggt cgccaccgcc atcagtctgt tcggtttcga
     1981 atcgggcgcc gcgctcgcca ccgtggtggg ggtgctggtc gaggtgccgg tgatgctgct
     2041 ggtggtgcga ctggtgaacc gcagccgcgc ctggtatggg cctgcttcag cgtagttcgt
     2101 cgaccttcag ccagatgccg aagctgtcgc tgccaccctc ggtggcacgg tagccgctgc
     2161 catcgcgccc ttcgctcgat cgctggtcac tgccgccgag gttgatccac tcgcccagcc
     2221 ggcccgagac ggtggtggag atctcccggg tttcggtggc gccgggctgg tcgccggccc
     2281 gttggtggag tggcgagatg tcgagggtga cccggtcgcc cgccagttgc ggtgcggcgt
     2341 agaagccggt gccgatgtca tgccactggg tggtttcgct gaccaccgcg ccgtccggtc
     2401 gcaggatcac ctgccgcatc ggcagcggga tcgactggcc gacctggatg aaggcgcgcc
     2461 ctccctccat cacctgcacg cgctggtcga tctcctcctc gctgcgggtc gatgcctgaa
     2521 agccgctggc ttcgatctgg ctgttgccgc ggttgatctc cacagtgccg ctcttcggca
     2581 gcgtcgaacc cgagcggatg atgcgcacgc cgctgccgcc cacgctgccc gacagttcac
     2641 cgccctgccc gtgccgctgt gcgctgtcgc catgacgcac gctgatcagc agttggcgcg
     2701 ccggccggtc gaggttctcg atcagccgca tgatctcggc gcggttgccg ctggaggcgc
     2761 gcaggtagag ggtgtcgccg cggccattga tgacgccggt cggttcgacg aagggggtca
     2821 gttgcggcag cagttgctcg gcggtgcggt gtctgagcgg gatgatctcc agccgctctt
     2881 ccgccgtgct ccacacggcc agcggcagca gaaccatcag gagcagaatg cggccgacca
     2941 tcgggagacc tcgcagggtg agaagaagcg gcactggatg ccattcaagc atagccaacc
     3001 cgtcgacgca gcgttgtcgg ctacaatcgc ccgaccattt gtcctggagc cgttgtgtgc
     3061 cacgcccgac cggattgaat cgatcttcac actggccgct ggccggcatg gtgtggctgc
     3121 tgctgacgct gggcggttgc gccactgtgg tgccacccac cggcgcgcct gccaccgccc
     3181 tcccctcgcc gacacccccg ccctcccctg ccgcacccgc gccagagcgc gcacgcggct
     3241 ggtggtatgc ctgcagccag ttcaactggt cgagcgacag tgaaccggat tgggggctcg
     3301 atctgttcgt cgccgatcgg gtggtgcggc cgttgatcgc cgcccaccgc gaccagttgc
     3361 cgctgtggcg tgtgcaccgt cgcgccgcgc ccgatggcgc tggccaccgc ttctgcctga
     3421 tcttctacgc gacgccgcag gtggccgggc agatcaacca ggagtggaac acgaatccgc
     3481 agctgcaacg gctgcaggag ggggggcagt tgcgggcgat tcaactcgat gacccgaccc
     3541 gcaacagccg gccggccctg gccgatgcca gcgacccggc ctggccggcg gtgatcggca
     3601 acgcctggcc ctactacatc atgggtgcca gtcagatgtg gctggagctg atcacgcaga
     3661 tgcgcagcga gcgcggcagc cccgacacca ccgagctggc gcagcagctc gaacagtacc
     3721 ggcaactgca ggggcagatc agccagctct ggaacgacag cgggcagcat gcttttctgc
     3781 accacctgaa tgcgctgttc ggctaccagc cggtgctgat tcgcgaacag cgctcctacc
     3841 gcttctgagc ggtgccggcc cggcggcaag ccggctcagt cgcgccggaa ctgcaatggc
     3901 agcgcggtga tgtcggcaac cggctgaccg cgccgataga gaatctggcc attcaccacc
     3961 gtggtgtgaa tcgaatgggc gaagcggtga ccctcgaagg gtgaccagcc gcacttcgac
     4021 agcaccttgg tccgggtcac ctcggtcggc gtcagcggat cgaccagcac caggtcggca
     4081 tggtagccct cgcgcagata gccacggtcg cgaatcgcaa acagctcggc gacccggtgg
     4141 ctgctcttgg cgaccacctg ctccagcgtc aactgaccct gctgcaccag ttcgagcagc
     4201 accagcaggg catcctgcac cagcggcaac ccggaagggg cctgggcgta gggacgggcc
     4261 ttttcatcga gcagatgcgg tgcgtggtcg gtggcgatga tgtcgatgcg gtcgtcgcgc
     4321 accgcctgca gcagcgccaa ccggtcgctc tcctgtttga tggcggggtt gcacttgatc
     4381 agcgtaccct tgtttcgata gtcgccatcg ttgaagaaga ggtggtgcac gcagacttcg
     4441 gcggtgatcc gcttgtcggc gagcggacct gcgctgaaca gcgccagctc gcgcgccgtg
     4501 gtcaggtgca gcacatgcag ccgggtgccg tgccgcttcg ccagctcgac cgccagcgag
     4561 ctggagcggt agcaggcttc ttcggagcgg atcagcggat ggcattcgat cggaatctgc
     4621 tcgccatgct gctgccggaa ccgcgcttcg ttggcgagga tggtcggggt gtcttcgcag
     4681 tgggtggcga tcagcagcgg gctgttggcg aagatctgct ccagtgtttt gggatcatcg
     4741 accagcatgt cgccggtcga ggcgcccatg aacaccttga tgccgcaggc gagccgggtg
     4801 tcgagcgcga ggatctccgc cagattctgg ttgctggcgc cgaaatagaa ggcgtagttg
     4861 gccaccgact tgctggctgc cagccgatgc ttctccagca gcgcctggtg gctggtggtg
     4921 ggtggctggc agttgggcat ctccatgaag ctggtgatgc cgccggccac ggcggcgcgc
     4981 gactcgctgg ccatgtcgcc cttgtgggtc aggccgggct cacgaaagtg cacctgatca
     5041 tcgatcagac cgggcagcag cagcaggccg ctggcatcga gggtggtgcg cgccggccgg
     5101 tgctgcaggt cgccgcccat ggcctcgatg cggccgtcac ggatcgccag atcggtttcg
     5161 ccgatcaccc cttcgttgac catgcggcca ttgagaatca gcagatcgag catggagtgg
     5221 ctgccatcgg agggaatggg gctgttattc tagcgcgatt gccccagaag gagttccgtc
     5281 atgtcccgct ccagcctgtt caagcagctc gaagttgcgc cacgcgccag ccaggtggcg
     5341 gtcggtgatt ttctggcggc gctcaacttc gaccaggccg ggctgattcc ctgcatcacc
     5401 cagcagcatg acagcggcga ggtgctgatg ctggcctgga tgaaccgcgc cagcctcgag
     5461 gagagtctgg ccagcggtcg gctctgctac tggtcacgct cgcggcagcg gctgtggcgc
     5521 aagggcgaga gctccgggca gttccagtgg ctgcgcgagc tgcgggtcga ctgcgacggt
     5581 gacgccctgc tggcactggt cgagcaacat ggggtggcct gccacaccgg gcggcagggc
     5641 tgcttctatc tgcgattgag tgcccagggc gccgaggtga tgcgcgatcc ggcggtgccg
     5701 gtcgagcagc tctacggtgc ggccaaggcg cagcaggaaa gcgagtgacc gctcggcagt
     5761 cggatgcggt cccgcagcgc cactactccc cgactgtcgt ggcgaccttg cctccgtggc
     5821 tgtggatggc cgcattgacg ggaaccgcca gcgatggagt caaacggcgc agctgttcga
     5881 gcagcggctg gctggggtag ccatcgaccg gcagcccatg caggctctgg aactgcttga
     5941 tggccgtctg ggtgcgaccg cccagcaggc catcctgctg gcccagctca taccccagaa
     6001 tggtcaaccg ctgctggatc tcgaccgctt cggcacggcg atagaaggtc tgcaccggtg
     6061 gcctggcgtg cagttccggc ccgccggcca gccggtcggc gaggtggccg accgccagcg
     6121 cgtagtagct ggaggcattc cagcgcatca ccaccttgaa gttgtcatag accagaaaac
     6181 agggcccctc gtagccttcg ggcgtcagca gcgcagcctg acgcccatcc tcgggcagcg
     6241 gtgaaccatc gacctggcgc acgccgagcc gtcgccattc gctcattggg cgccagccct
     6301 tgatgccggt cagattccaa ggcagatcgc gcggcacccg cacttcgcga ccccagcggt
     6361 cctgctcctt ccagccgatg cggttcaggt agttggcggc ggaggcaaag acatcgggca
     6421 ggtcattcca caggtcggtg cggccattgc cgtcgtaatc gaccgcatag agcgcatagg
     6481 tgccgggcat gaactgcacc tgccccatcg cgccggccca ggagccgcgc atcgactccc
     6541 gggcgatctt gccctggtcg accagccgca gcgcttcgac gaactggcgg ccgaacatgc
     6601 tgctgcggcg ctggtcgcag gccagcgtgg ccagcgcatc gagggtcgga atgtcgccga
     6661 gatagcggcc gaagttggtt tcgagtcccc acagcgccac cagatagtgg tagggcacgc
     6721 cgtactggcg gctgatctgc tccagcagct cgcggtgggt ggcgatctgg ttgcgaccgg
     6781 tctcgacccg ttccggcgac acgctgcgcc ccagatagtg ccagaaggtg tagatgaact
     6841 ccgattgccc accgcggtcc ttctgcaaca cctcgggacg ctggctgatg tcggcaaagg
     6901 ccagatcgag ggtggcgccg tggatgccct ggctgctggc ctgctgtcgc agggagtcga
     6961 gacagaggtt gaagcgctgc tcggcagcgg ccggcagcgt gagtacaagg agcagcagcg
     7021 catgagcggt ggcgagcagg cggcgcatgg tgac
//
LOCUS       DAORCQ010000112         6948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_7755_length_6948_cov_5.046134, whole genome shotgun sequence.
ACCESSION   DAORCQ010000112 DAORCQ010000000
VERSION     DAORCQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7755_length_6948_cov_5.046134"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..721
                     /locus_tag="PKI23_10090"
     CDS             299..721
                     /locus_tag="PKI23_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNN37325.1"
                     /translation="MKVSAIMSSPVVTVHLDDRLSAVREIFSRTRFHHLIVVEHGKLF
                     GVVSDRDLLKAISPNIGKGAERDADTATLNKRVHQIMSRKPIFLQEESSVLDAIRLFD
                     EHAVSCIPIVNPDNTPVGILSWRDIIKAIRAVSQKEKP"
     gene            complement(752..1696)
                     /locus_tag="PKI23_10095"
     CDS             complement(752..1696)
                     /locus_tag="PKI23_10095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNN37326.1"
                     /translation="MSAVLSIPTPSRDASLPGSGGGTADAIAAELAELNLQLGLARLL
                     QTSLEVEQLLTFFADWVSKIMPITGLGYRHEDEQIEFAWEGDGCIQLNYRLAIEQMAL
                     GEIGLHRNHPFSEAETELLETLLSTLIFPLRNALIHRKTLRTALTDGLTQVGNRQAFE
                     SAMRREVDLARRHRQPLSLLMIDVDHFKRINDSHGHPAGDQVLSELAAEIGRAARSTD
                     LLYRYGGEEFALLLHNTPAEGALITAERIRRAAAKLEVTYRGSVLNFTVSLGIATLHD
                     DSAAELLRRADEALYRAKHEGRNRVCMAEEAATDQLSR"
     gene            complement(1926..2687)
                     /locus_tag="PKI23_10100"
     CDS             complement(1926..2687)
                     /locus_tag="PKI23_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HNN37327.1"
                     /translation="MFEYQPPSDLLQGRFILVTGAGAGIGRAAANCYARHGATVVLLG
                     RTVKHLEECYDEIVAAGGPEPAIYPMNLQGATLRDHQDLTATLEREFGQLHGVLHNAA
                     LLGRRTPLGQYPLDEWQKLLQVNLTAAFMMTQALLPLLMQADDASVIFTSSGVGRRGR
                     AYWGAYAVTKFAVEGMAQVWAEELGSVSQVRINSINPGATRTRMRADAYPGEDPARNP
                     APDEIMPLYLYLMGPQSRHLNGVSLDAQTRDGQPG"
     gene            complement(2751..3428)
                     /locus_tag="PKI23_10105"
     CDS             complement(2751..3428)
                     /locus_tag="PKI23_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNN37328.1"
                     /translation="MRTLSTVLFDLDGTLLDTAPDFIVVLRQLMQQRQCQRLLSDEAI
                     RATVSEGAGALITLAFGHAPDAAEFEPLRQQLLDLYQRNGHARTRLFAGLAELLDWFD
                     ATGIAWGVVTNKPSHYAVPLMQGLDLLPRCRTLVCPDHVTRRKPDPEPILLACRQLDR
                     PVAHTLYIGDHRRDIEAGRAAGALTMAAAYGYVHPADPAEQWQADHLVRSVDEMASWL
                     RQRLSAD"
     gene            complement(3434..4144)
                     /gene="ubiG"
                     /locus_tag="PKI23_10110"
     CDS             complement(3434..4144)
                     /gene="ubiG"
                     /locus_tag="PKI23_10110"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011103693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNN37329.1"
                     /translation="MQHANNVDLGEIAKFEALANRWWDPDSEFKPLHDINPLRLNYID
                     ERAGLAGRRVVDIGCGGGLLAEGMAVRGAEVLGIDMGEAPLAVAELHRLESQLEGLSY
                     RRVTAEALADEQPAAFDLVTCLEMLEHVPDPLSVIRAAARLVKPGGHLFFSTINRNPK
                     SFLFAIVGAEYLLGLLPRGTHQYTRFIRPAELAGWLRMADLRLDDLTGMVYNPLTRRY
                     RLDRDVDVNYLLHAVKGA"
     gene            4463..5338
                     /locus_tag="PKI23_10115"
     CDS             4463..5338
                     /locus_tag="PKI23_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNN37330.1"
                     /translation="MQPFDYVAPTSLAAATELLKSASSVPTRILAGGTDLLVQMRAGA
                     RSTSRVLDIKKIPETSVLEIGTSHLRIGAAISAAQICGHPELRKLFPGVMESIELIGS
                     TQVQGRATPVGNLCNASPGADSVPALVAVGAEARVVSASGARWLPVAELPAAPGRTTL
                     APEEFVVELRLPLPAARSSDAYLRFTPRAEMDIAVVGVGVALTLDEQGVCRAARAAVG
                     AVAATVLLAPTVADAIIGSKIDDESLARAAQAATRVGNPISDKRGTAEFRRHIAGVLA
                     KRAIRAAAQRAQQQG"
     gene            5344..5826
                     /locus_tag="PKI23_10120"
     CDS             5344..5826
                     /locus_tag="PKI23_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNN37331.1"
                     /translation="MAKQHVSTTINGEAIEFLCEPQQSLLEVLRDELGLTGTKEGCAT
                     GDCGACSVQLNGRLICSCLMLGVEAQGQQIGTIEGIAKPHELHPIQQKFLEHAALQCG
                     ICTPGYIVATKALLEQNPDPTEQEVRYWLSGNLCRCTGYDKIVRAVLDAAAELKGATP
                     "
     gene            5823..>6948
                     /locus_tag="PKI23_10125"
     CDS             5823..>6948
                     /locus_tag="PKI23_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNN37332.1"
                     /translation="MSEQSTPKTYRSIGTSPVRPDGIDKVTGRANYGADRHLPGMLHG
                     KTLRSPHAHARIRSIDVSEAEKLPGVMAVVTGADFPHLQSAMEEAGESQIDVWDLARN
                     VMARDKVLYHGHAVAAVAATSDAIAEAALAKIRVDYEVLTPVMDIEAAMADNAPLLHD
                     EMRTQGLPAAADRASNVATVIKLARGDLAAGFAAADVVIEREFSTPTVHQGYIEPHAV
                     VADCRENGQATLWCCTQGPFVVRTLTSKVLNWDVSRIKVIPSEIGGGFGAKTTVYQEP
                     VAIMLSLKSGRPVKMVMNREEVFRATGPTSASRIRIKLGANRAGKIMAAEAELKYEAG
                     AFKGSPVMPGCMCVFVSYEMQSFSITGYDVVVNKPKVAAYR"
BASE COUNT         1275 a         2236 c         2224 g         1213 t
ORIGIN      
        1 gaccacggtt tcctggtttc tgctccgctt tggcagaaaa ccgtaacggg aaaccatggt
       61 ctgtccccaa ttctacaggt gttggcgtga cattcgctgg ttgctgcagg gcaaaaaagg
      121 cagaggactt tcgtcagatg agccagtccg gagacagcgc gtgacccaca gttcgctgag
      181 aaaatgaagc cagactgacg cccgtttcag cacccggtcg tgctcgatca atgctgcaca
      241 gcaaagctga ggctggcggc tagactttgc tgtgtgactg cgtcttcagg gggcaacagt
      301 gaaagtcagc gcgatcatgt cttcaccggt ggtgacagtg cacctcgacg acaggctgtc
      361 ggcagtgcgg gagattttca gccggaccag attccatcac ctgatcgtgg tcgagcatgg
      421 caagctgttc ggcgtcgtct cggatcggga tttgttgaag gcgatcagcc cgaatatcgg
      481 caaaggggcg gagcgtgatg cagacaccgc gaccctcaac aagcgggttc accagatcat
      541 gagccgcaag ccgatctttt tgcaggagga gagcagcgtg ctcgatgcca tcaggctgtt
      601 tgacgaacac gccgtctcat gcattccaat cgtcaacccc gacaacacgc ctgtgggcat
      661 tctcagttgg cgcgacatca tcaaggcgat ccgggccgtc agtcaaaaag agaagccgta
      721 gcagcagtcg agcgacagcg gcaaaaaccg gttaccgaga caattgatct gtcgctgctt
      781 cctcggccat gcagacgcgg ttgcgcccct cgtgcttggc ccggtagagc gcttcatcgg
      841 cgcgccgcag cagttcagcc gctgagtcgt cgtgcagcgt ggcgattccc aggctgaccg
      901 tgaagttcag cacgctgccg cggtaggtga cctcgagttt ggccgccgcc cggcggatgc
      961 gctctgcggt gatcagtgcc ccttcggccg gtgtgttgtg cagcagcagt gcgaactcct
     1021 caccgccata gcgatagagc aggtcggtcg aacgggccgc ccggccgatc tcggcagcca
     1081 gttcgctcag cacctggtca ccggccgggt ggccatggct gtcattgatc cttttgaagt
     1141 ggtcgacatc gatcatcagc agcgacagcg gctgacgatg ccgtctggcg agatcgacct
     1201 cgcggcgcat cgcgctctcg aatgcctgac ggttgccgac ctgggtcaga ccatcggtga
     1261 gggccgtgcg cagcgtcttg cggtgaatca aggcgttgcg caacggaaag atcagcgtgg
     1321 agagcagggt ttcgagcaat tcggtctcgg cctcgctgaa ggggtggttg cgatgcaacc
     1381 cgatctcacc cagcgccatc tgttcgatcg ccagtcggta gttcaactgg atgcagccat
     1441 ctccctccca ggcgaactcg atctgctcat cctcgtgccg atagcccagg ccagtgatcg
     1501 gcatgatctt gctgacccag tcggcaaaaa aggtgagcag ttgctccact tcgagcgaag
     1561 tctgcaacag ccgggcaaga ccgagctgca gattcagctc cgccagttcg gcggcaatgg
     1621 cgtcggcggt accgccccca cttcccggca gggatgcgtc gcgcgaaggg gtcggaatcg
     1681 ataacactgc ggacatggat tcatcactcc agagagtctt ggcttgatcc gggcgtcacg
     1741 ctgatgggcg cctcgttgac acgactgctt tagcgaaagc cgtgccaact atcaagagac
     1801 tgttttccaa gctcaaaacc gtttttcagc cggtttattg gccagaaacg gcaggagtgg
     1861 tggttttttg accagaaagc cgccgctgcc ctccttcgct cggcgggtga tcagtcaaaa
     1921 tactgtcatc cgggttgtcc gtcgcgcgtc tgcgcatcca gcgaaacacc gttgagatgg
     1981 cggctctgcg gccccatcag gtagaggtag agcggcatga tttcgtccgg agccggattg
     2041 cgggccggat cttcgccggg ataggcgtcg gcccgcatcc gggtgcgggt cgcgcccgga
     2101 ttgatgctgt tgatccgcac ctgactcact gagcccaact cctcggccca gacctgcgcc
     2161 atgccctcga cggcaaactt ggtgaccgca taggcacccc agtaggcacg tccgcgtcgg
     2221 ccaacgcccg acgaagtaaa aatcactgaa gcatcatcgg cttgcatcaa cagcggcagc
     2281 agcgcctgcg tcatcatgaa ggcggcagtc agattgacct gcagcagttt ctgccactca
     2341 tcgagcgggt actgcccaag cggcgtgcgc cggcccagca gcgcggcatt gtgcagcaca
     2401 ccatgaagct ggccgaactc ccgctccagg gtcgcggtca gatcctggtg gtcgcgcagg
     2461 gtggcgccct gcagattcat cggatagatg gccggctcgg gtccaccggc ggcgacgatc
     2521 tcgtcgtagc actcttcgag atgcttgacc gtgcgcccca gcaacaccac ggtggcgccg
     2581 tggcgcgcgt agcagttggc cgcggcccgg ccgatgccgg caccggcgcc ggtcaccaga
     2641 atgaaacgcc cctgcaacaa gtcggaaggt ggttgatatt caaacatttg ctgtggcctc
     2701 ccagagactc ggtaaattcg cgaacacggc aactccctgc cgcccgggtt tcagtcggcg
     2761 gagagccgtt gccgcagcca cgatgccatc tcatcgacgc tgcgcaccag gtggtcggcc
     2821 tgccactgct cggccggatc ggccggatgc acgtagccgt aggcggccgc catcgtcagg
     2881 gcgccggcgg cccggccggc ctcgatgtcg cggcggtggt cgccgatgta gagcgtatgc
     2941 gccaccgggc gatcgagctg gcgacaggca agcaggatcg gttcgggatc gggcttgcgt
     3001 cgcgtgacat ggtcagggca gaccagagtg cggcagcgcg gcagcagatc gagcccctgc
     3061 atcaatggca ccgcatagtg actcggcttg ttggtcacca cgccccaggc aatgcccgtt
     3121 gcatcgaacc agtcgagcag ttcggccagg ccggcgaaga gccgggttcg cgcatggccg
     3181 ttgcgctggt aaagatcgag caactgctgg cgcagcggct cgaactcggc tgcatcgggc
     3241 gcatgcccaa aggccagggt gatcagcgcg cccgccccct ccgacaccgt cgcgcgaatc
     3301 gcctcgtcgc tgagcaggcg ctggcactgc cgctgctgca tcaactgccg cagcaccacg
     3361 atgaagtcgg gggcggtatc gagcagggtg ccatcgaggt cgaacagcac cgtggagagc
     3421 gtccgcatcg gcttcacgcc cccttcaccg catggagcag atagttgacg tcgacatcgc
     3481 ggtcgagccg gtagcgccgg gtcagcgggt tgtagaccat gccggtcagg tcatccagcc
     3541 gcagatcggc cattctgagc cagccggcca gctcggccgg gcggatgaaa cgggtatatt
     3601 gatgggtgcc gcgcggcagc agacccagca gatattcggc gccaacgatg gcgaacagaa
     3661 aggatttcgg attgcggttg atggtcgaga agaacagatg accgcccggc ttcaccagcc
     3721 gggccgcggc ccgaatcacc gacagcggat cgggcacatg ctcgagcatc tccagacagg
     3781 tgaccaggtc aaaggcggcc ggctgctcat ccgccagcgc ctcggcggtc acccggcggt
     3841 agctcagccc ttccagttgc gattcgagcc gatgcagctc agccaccgcc agtggcgcct
     3901 cgcccatgtc gatgcccagt acctcggcgc cgcgcactgc catcccctcc gccagcaggc
     3961 caccaccgca gccgatgtcg accacccgcc tgccagccag ccctgcccgc tcgtcgatgt
     4021 agttgagccg cagcggattg atgtcatgca gcggcttgaa ttcgctgtcc gggtcccacc
     4081 agcggttggc cagggcttcg aacttggcga tctcacccag gtcgacattg ttggcgtgct
     4141 gcatgggggc tcctgtggct gtgcaatggt ggcatgatac cgcaagcccc cagccatcgc
     4201 gtcactcagc gtcggtcgca ccgtgcactt cgccggccct tttcgccggc cgatttctgt
     4261 ttatataatg cggtgtcctc aagttggaat tgctgcgcgc gatgcgtggc gttccccatc
     4321 cttgctgccg aacagaacaa ccgattcaga gcgacgcact ttcgctccga tcgcggatat
     4381 aacaacgagc gcgcgcggcc tggtctggat caatatgccc gacgccgccg tcgctcgacc
     4441 acaggctcat tagggaggct tcatgcaacc tttcgactat gtggcgccca cttcactggc
     4501 cgcggccact gaactcctca agagcgcctc atccgtgccc actcgcatcc tggccggcgg
     4561 gactgacctg ctggtgcaga tgcgtgccgg cgcgcgcagc acttcgcgcg tgctcgacat
     4621 caagaagatt ccggaaacca gcgtgctgga gatcggcacc agccacctgc gcatcggtgc
     4681 ggccatcagc gccgcgcaga tttgcgggca tcccgagctg cgcaagctgt ttcccggcgt
     4741 gatggagtcc atcgaactga tcggttcgac ccaggtgcag ggccgggcca cgccggtcgg
     4801 caacctctgc aacgcatcac ccggtgccga cagtgtgccg gcactggtgg ccgtcggcgc
     4861 cgaggcgcgt gtggtcagtg ccagtggcgc gcgctggcta ccggtggccg agctgcccgc
     4921 tgcgccagga cgcacgacgc tggcgccgga ggagttcgtc gtcgaactgc ggctgccgct
     4981 gcctgccgca cgcagctccg atgcctacct gcgcttcacg ccgcgtgccg agatggacat
     5041 tgccgtcgtc ggtgtcggtg ttgcgctcac cctggatgaa cagggcgtct gccgcgcggc
     5101 gcgtgccgcc gtcggcgccg tggccgcaac ggtactgctg gcgcccaccg tggccgacgc
     5161 catcatcggt tcgaagatcg atgacgagtc gctggcacgg gccgcccagg ccgccacgcg
     5221 ggtgggcaat ccgatcagcg acaagcgagg caccgcggaa ttccgccgcc acatcgccgg
     5281 ggtgctggcc aagcgcgcca tccgcgccgc ggcacaacgg gcgcaacaac aggggtaatg
     5341 aagatggcga agcaacatgt ctccaccacc atcaatggtg aagcgatcga atttctctgt
     5401 gagccgcagc agagcctgct ggaggtgctg cgcgacgagc tggggttgac cggcaccaag
     5461 gagggttgtg cgaccggcga ctgtggcgcc tgctcggtgc agctcaatgg ccggctgatc
     5521 tgctcctgcc tgatgctggg cgtcgaggcc cagggacagc agatcggcac catcgagggc
     5581 atcgccaagc cgcatgagct gcacccgatc cagcagaagt tcctcgaaca tgccgccctg
     5641 cagtgcggca tctgcacgcc cggctacatc gtggcgacca aggcattgct ggaacagaac
     5701 cccgatccga ccgagcagga ggtgcgttac tggctctccg gcaacctgtg ccgttgcacc
     5761 gggtatgaca agatcgtgcg tgcggtgctc gatgccgccg cggaactcaa aggagcgaca
     5821 ccatgagcga gcagagcact cccaagacct accgatcgat cggcacctcc ccggtccgcc
     5881 ccgatggcat cgacaaggtg accgggcgcg ccaactacgg tgccgaccgc cacctgcccg
     5941 gcatgctgca tggcaagacg ctgcgcagcc cgcatgccca tgcgcgcatt cgctcgatcg
     6001 atgtcagcga ggccgaaaag ctgccgggcg tcatggcggt ggtgaccggt gccgacttcc
     6061 cccatctgca atcggcgatg gaagaggccg gcgagtcgca gatcgatgtg tgggatctgg
     6121 cccgcaacgt catggcgcgc gacaaggtgc tctaccacgg ccatgcggtg gccgcagtgg
     6181 cggccacctc cgatgcaatc gccgaggcgg cactggcaaa gattcgggtc gactatgaag
     6241 tgctgacacc ggtgatggac atcgaggccg ccatggccga caacgcaccg ctgctgcatg
     6301 atgagatgcg cacccagggg ctgcccgctg cggccgatcg cgcctccaac gtcgccacgg
     6361 tgatcaagct ggcgcgtggc gaccttgccg ccggctttgc agcggccgat gtggtgatcg
     6421 agcgtgaatt ctcgaccccc accgtccacc agggctatat cgagccccat gccgtcgtgg
     6481 ccgactgccg cgaaaatggt caggccacgc tctggtgctg cacccagggc cccttcgtgg
     6541 tccgcacgct gaccagcaag gtgctgaatt gggatgtctc ccgcatcaag gtgattccga
     6601 gcgaaatcgg cggcggcttc ggtgccaaga ccaccgtcta tcaggagccg gtcgcgatca
     6661 tgctgtcgct gaagtcgggc cgtccggtca agatggtgat gaaccgcgaa gaggtgttcc
     6721 gcgccaccgg tcccacctcg gccagccgca tccgcatcaa gcttggcgcc aaccgcgccg
     6781 gcaagatcat ggccgccgag gccgagctga agtacgaagc gggcgccttc aagggctcgc
     6841 cggtgatgcc cggctgcatg tgcgtgttcg tctcctacga gatgcagagc ttcagcatca
     6901 ccggctatga cgtggtggtc aacaagccga aggtggcggc ctatcggg
//
LOCUS       DAORCQ010000113         6869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_7882_length_6869_cov_4.807455, whole genome shotgun sequence.
ACCESSION   DAORCQ010000113 DAORCQ010000000
VERSION     DAORCQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_6869_cov_4.807455"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="PKI23_10130"
     CDS             complement(<1..1973)
                     /locus_tag="PKI23_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNN37333.1"
                     /translation="MKRILINAAHPEELRVALVDGQKLYDLDIEHRTREQTKANIYKG
                     RITRIEPSLEAAFVDFGAERHGFLPLKEISRDYPGLKEGHQVIVQVEKEERGTKGAAL
                     STFVSLAGRYLVLMPNNPRGGGISRRIEGEERAELREIMSQLNLAEGMGVIVRTAGIG
                     RSLDELQADLDYLVQLWQAITQAGAERPAPFLIYQESNVVVRAVRDYLRPDIGEVLID
                     SEEAYQEACAFIRQVMPQYEPNIKLYRDAIPLFTRYQIESQIEVVYQREVKLPSGGMV
                     VIDPTEALVSIDINSARATRGADIEETALQTNLEAADEIARQLRLRDIGGLIVIDFID
                     MSAARNQKEVENRMRQALEMDRARVQVGRISRFGLLEMSRQRLRPSIEEASHISCPRC
                     NGQGTIRGMKSLSLSIMRILEEETTKGGSAEIRAIVPVPVAAYLLNEKRNNLLEIEQR
                     SKIRLRIIPNPNLETPLFRLERLRQGESIEAPDYESTEILLEELEEESSQQSRPRKQA
                     AVGPIAAAPSAPAAATATAAPAARPRGLLKRLTSALSELFEPATEAAQPESNSRTTQS
                     AATAAASAPNLKPAGRPTPAATETETGRENRQRDHRPSGREQRSDGGRGGRGDGRGQQ
                     QQSRRPERPPREPAAQKESSPPAAAEPRSNQEQT"
     gene            complement(1915..2268)
                     /locus_tag="PKI23_10135"
     CDS             complement(1915..2268)
                     /locus_tag="PKI23_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37334.1"
                     /translation="MMYIKLPTANSSSSHEEIQNLSMVHRLFFMLAQPLRHCAVLFCA
                     LIARHFEPWPASPLQRPTWTLSIRHGFAATRSLVGSQPFPQFLTAQIHRTSESHHEKN
                     SDQCGPPRGAARRPR"
     gene            2604..3548
                     /gene="rluC"
                     /locus_tag="PKI23_10140"
     CDS             2604..3548
                     /gene="rluC"
                     /locus_tag="PKI23_10140"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HNN37335.1"
                     /translation="MEIRTIQAEEAGQRVDNFLLRALPGVPKSKIYNIVRRGEVRVNQ
                     GRVKPSYRLQDGDALRIPPVRVGVRDEVPAAPRQLQALAQMVLHEDDELLVLNKPAGL
                     AVHGGSGVSLGLIELLRQQRPEARFLELVHRLDRDTSGCVMVAKRRRALLELQQALQS
                     GQVDKRYQLLVAGAWPAAVRRVDAPLRKNILSSGERLVRVDSEGKPACTEFRVLERFA
                     QATLLEARPITGRTHQIRVHARQQGHPIAGDPKYGDAQFDAALRGQGLQRLFLHAWRL
                     ELKLPSRNAPLRLEAPLEASLAAVLARLAKAGTDASPG"
     gene            3602..4603
                     /locus_tag="PKI23_10145"
     CDS             3602..4603
                     /locus_tag="PKI23_10145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNN37336.1"
                     /translation="MSDDQWREDEPSAPRRPPAESSKEWRLIEKALLAGIGEQRRARR
                     WGIFFKFLTFGYLFVLLGLFSRSTHLDEKVGVAGRHTALIDVQGVIAEGEEAQADSIV
                     TALRSAFDEKHVAGIVLRINSPGGSPVQAGYVYDEIKRLRALHPQVKVYAVIADIGAS
                     GAYYIAAAADQIYADKASLVGSIGVVSSSFGFVDALKKLGVERRLFTSGEHKGFLDPF
                     SPLNPQEQAYWQQVLDVTHRQFIEQVKAGRGSRLNSEKSDLLFSGLVWSGEQARELGL
                     IDGLGSPGYVAREVIGAEQIVNYTEKPTPFEKLARRMESRLDGAALTSDLFRTLPGW"
     gene            complement(4619..>6869)
                     /locus_tag="PKI23_10150"
     CDS             complement(4619..>6869)
                     /locus_tag="PKI23_10150"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HNN37337.1"
                     /translation="AQDRLNYRPTVQTLSGFIRNAGTEPHLTLAITGEWGQGKSSVMR
                     MLKRDLEQAGFRTAWFNAWHHQQEGRPMSALFNVIRRQAMPSLFSREAIAALRVRWRL
                     LWGRGGAYRFTLPVMLFGLLLTGQDVVHDYTHEGIVLSGGERLRWSASHHLLGEQRVR
                     LTPESLARLTPVAPSSPAPTDNNTAKGTPAPTATPADKSKSAPPDNKTVKPPPAPAET
                     AAADSLRVRQALIDYLRTSLVYGGGGGDADTGCRDPRPLPPALRCVFDHSQQLFATID
                     QHAGFTLTVDERQHLLKVATTLPAPPLLPRMEWLLLSLGPLLALLLTKGVSVYGFQLL
                     RPLNGLVNRLTGQSADEKEPTGLIEHYRTEFALLTQALDGRLVLFVDDLDRCSASTVN
                     SLLELTNYLTDIGQCFIVLGVAIEHIEGSIASNREGTDNTEYARRYLRKLIHVEVPVP
                     HRMAQTDRLYDPPAGDSPGWLGRLLRLLGRWLPLRLLLGGPAVIGVVLLALPAFDWMN
                     ATLGVAQLPLDIPAVQSQTAVAASPAASAATNPPPTPKTPSVDAGAPMLSTATRVPES
                     VTFGIAGGALIGTLLLILAQRLFPGVASSVNRALGRAERTFDSAAFVEALRLWHPALV
                     QYDPTPRGVKRFANRARLMVLYERLSAKGTSRQALNEAQVVAVTAIHQVAPELLDRWA
                     NAGGESTGSTGFNLGWQVANSATTGTATPQLYPELLNCFKEHCKEHGEPAAADIARLN
                     RRMQDVKVR"
BASE COUNT         1090 a         2191 c         2373 g         1215 t
ORIGIN      
        1 gtctgctcct ggttgctgcg cggttccgct gcggccggcg ggctgctctc tttctgcgcg
       61 gcgggctccc tgggcgggcg ttccggacgg cgcgactgct gttgttgccc acggccgtca
      121 ccgcggccgc ctctgccacc gtcgctgcgc tgctccctgc cggatggacg atggtcgcgc
      181 tgacggtttt ctctgccggt ttcggtttcg gttgcggccg gagtcgggcg tccggccggt
      241 ttcaggtttg gagccgatgc tgctgcggtc gcggccgatt gcgtagtgcg gctgttgctc
      301 tcgggttggg ccgcttcggt tgcgggctcg aacagttcag agagggcgct ggtgagccgc
      361 ttcagcaggc cgcgcggtct ggcggcgggc gccgcggttg cggttgcagc ggccggtgcc
      421 gagggtgccg ctgcaatggg gcccacggcc gcctgcttgc gtggccggct ctgctggctg
      481 ctctcctctt cgagctcttc gagcaggatc tcggtcgatt cgtagtcggg agcttcgatc
      541 gattcaccct gccgcagccg ttcgaggcgg aacagcgggg tctccaggtt ggggttgggg
      601 atgatgcgca ggcggatttt gctgcgctgc tcgatctcca gcaggttgtt gcgcttctcg
      661 ttgagcaggt aggcggcgac cggcaccggc acgatggcgc gaatttcggc gctgccgccc
      721 ttggtggtct cctcctcgag gatgcgcatg atcgacagcg acagcgactt catgccgcgg
      781 atcgtgccct gcccgttgca gcgcgggcag gagatgtggc tggcctcctc gatcgacggg
      841 cgcagccgct ggcgggacat ctccagcagt ccgaaacggg agatgcgccc cacttggacg
      901 cgcgcgcggt ccatctccag tgcctggcgc atccggttct cgacctcttt ctggttgcgc
      961 gccgcgctca tgtcgatgaa gtcgatgacg atcagaccgc cgatgtcccg cagccgcaac
     1021 tggcgggcga tctcgtcggc ggcttccagg ttggtttgca gtgcggtctc ttcgatgtcc
     1081 gcgccacggg tggcacgcgc cgagttgatg tcgatcgaga ccagcgcctc ggtcgggtcg
     1141 atcaccacca tgccgccgga gggcagcttc acttcacgct gatagaccac ctcgatctgg
     1201 ctttcgatct gatagcgggt gaacagcgga atggcatcgc gatagagctt gatgttcggc
     1261 tcgtactgcg gcatcacctg gcggatgaag gcgcaggctt cctgataggc ctcctccgag
     1321 tcgatcagca cttcgccgat gtcggggcgc aggtagtcgc gcaccgcacg cacgaccaca
     1381 ttgctctcct gatagatcag aaacggcgct ggccgctccg cgcctgcctg ggtgatcgct
     1441 tgccacaact gcaccaggta gtcgaggtcg gcctgcaact catccaggct gcggccgatg
     1501 ccggcggtgc gcacgatgac gcccatgcct tcggccagat tgagctggct catgatctcg
     1561 cgcagttcgg cgcgctcttc accctcgatg cggcgggaga tgccgccgcc gcgcgggttg
     1621 ttcggcatca gcaccagata gcggccggcc aggctgacga aggtgctgag tgcggcgccc
     1681 ttggtgcccc gctcctcctt ctcgacctga acgatgacct ggtggccctc tttcaggccg
     1741 gggtagtcgc gcgagatctc cttcagcggc aaaaagccgt gccgctcggc gccgaaatcg
     1801 acaaaggctg cctcgaggct gggctcgatc cgggtgattc tgcccttgta gatgttggcc
     1861 ttggtctgct cccgcgtgcg gtgctcgatg tccaggtcgt agagtttctg cccatcaacg
     1921 agggcgacgc gcagctcctc ggggtgggcc gcattgatca gaattctttt catggtgact
     1981 ttcgcttgtt cggtgaattt gtgcagtcaa aaactgcggg aagggttggc tgccgaccag
     2041 cgacctcgtt gcggcgaatc catggcggat cgacagcgtc cacgtgggtc gctgcaaggg
     2101 cgatgcaggc cagggttcaa agtgtcgggc aatgagcgcg caaaacagca cggcgcagtg
     2161 gcgcagcggt tgcgcgagca tgaagaagag gcggtggacc atcgacaggt tctgaatctc
     2221 ttcgtggctc gacgacgaat tggcggtggg cagcttgatg tacatcatgc cgggttggcg
     2281 caatggaatc aggttggccg acaggagatg gtctggttgc ggattgcacg ccagggcatg
     2341 cggagcaaaa agcgcgacgg gaggtggaca actggcccgc tcgcccgacg ctggcccacg
     2401 gttggaccga gggtgctgtt gcgtccgccc ggtggcgcat tgaacggcca ccggctgccc
     2461 gctgcgaccg cgcctttgac aacatgcgac ccatcgggtt tgctaatata gcaccagtca
     2521 tcaaatttct tcaaacaggg cacgctgtca tcgccacgca caccccccag gagtcaggcg
     2581 cgtccgcgcg acgcccgccg gccgtggaga tcagaacgat ccaggccgaa gaggccggac
     2641 agcgggtcga caactttctg ctgcgcgcac tgcccggcgt gccgaagagc aagatctaca
     2701 acatcgtgcg gcggggtgag gtcagggtca atcagggccg ggtcaagccg agctaccggc
     2761 tgcaggatgg cgacgccctg cgcattccgc ccgtgcgggt gggcgtgcgg gacgaggtgc
     2821 cggcggcgcc gcgccagttg caggcgctgg cgcaaatggt actgcatgag gatgacgaac
     2881 tgctggtgct caacaagccg gccgggttgg ctgtgcatgg tggcagcggg gtctcgctcg
     2941 gactgatcga gctgctgcgg cagcagcgac ccgaagcccg gtttctggag ctggtgcacc
     3001 gcctcgaccg tgacacctcc ggctgcgtga tggtggcgaa gcggcgacgg gcgctgctcg
     3061 agttgcagca ggcgctgcaa agcggccagg tcgacaagcg ttatcagctg ctggtggcgg
     3121 gcgcgtggcc ggcggccgtg cgccgggtgg atgccccgct gcgcaagaac atcctcagct
     3181 ccggcgagcg gctggtgcgg gtcgacagcg aaggcaagcc ggcctgcacc gaattccgcg
     3241 tgctcgagcg ttttgcgcag gcgaccctgc tcgaggcgcg ccccatcacc gggcgcaccc
     3301 atcagattcg ggtgcatgcg cgccagcagg gccatccgat tgccggcgac cccaaatatg
     3361 gcgatgcgca gttcgatgcc gcgctgcgcg gtcagggcct gcagcggctg ttcctgcatg
     3421 cctggcggct cgaactgaag ctgccgagcc gcaacgcgcc gctcaggctg gaggccccgc
     3481 tcgaggcgag cctggcggcg gttctggcgc gactggccaa ggccggaacc gatgcgtcgc
     3541 cgggctgatc ctgcacacgg cagagactcg acacctcaat gaaccgaaaa ggtgactttt
     3601 tatgagtgac gaccaatggc gagaggatga accttccgca ccgcgccgtc cgccggccga
     3661 gagttcgaag gagtggcgtc tgatcgaaaa ggcgctgctg gcgggcattg gcgagcagcg
     3721 gcgtgcgcgg cgctggggca tcttcttcaa gttcctgacc ttcggctacc tctttgtgct
     3781 gctggggctc ttctcccgca gcacccacct cgacgagaag gtgggcgttg ccgggcgtca
     3841 caccgcgctg atcgacgtgc agggggtgat tgccgaaggc gaggaggcgc aggccgacag
     3901 cattgtgacc gcactgcgca gcgcctttga tgagaagcat gtggccggca tcgtgctgcg
     3961 catcaacagc cctggtggca gtccggtgca ggcgggctat gtctacgacg agatcaaacg
     4021 gctgcgcgcg ctccatccgc aggtgaaggt ctatgcggtg attgccgaca tcggcgcttc
     4081 cggcgcctac tacatcgcgg cggcagccga tcagatctac gccgacaagg cgagcctggt
     4141 cggctcgatc ggcgtcgtct cctccagctt cggttttgtc gatgcgctga agaagctcgg
     4201 cgtcgaacgg cggctgttca cctcgggcga gcacaagggc tttctcgacc ccttctcgcc
     4261 gctcaatccg caggagcagg cctactggca acaggtgctc gacgtgactc accgccagtt
     4321 catcgagcag gtgaaggccg gtcgcggcag ccggctcaac agcgagaagt ccgatctgct
     4381 gttcagcggt ctggtctgga gcggcgagca ggcgcgtgaa ctgggactga tcgatggcct
     4441 cggcagcccc ggctatgtcg cccgcgaggt gatcggcgcg gagcagatcg tcaactacac
     4501 cgagaaaccg acacccttcg agaagctggc gagaaggatg gagagccgcc tcgacggcgc
     4561 cgcgctgacc agtgacctgt tccggacgct gccgggctgg tgaagagaac ctgccggctc
     4621 agcgcacctt cacatcctgc atccggcggt tcaggcgggc gatgtcggca gcggcgggtt
     4681 caccatgttc cttgcagtgc tctttgaaac agttgagcag ttcagggtac agttgggggg
     4741 tagcggtgcc cgttgtggcg gagttcgcca cctgccagcc cagattgaat ccggtcgaac
     4801 cagtggattc accgcctgca ttcgcccacc ggtccagcaa ctccggagcc acctgatgaa
     4861 tcgccgtcac cgccaccacc tgcgcttcat tcagcgcctg gcgtgacgtg cccttggcac
     4921 tcagccgttc atacaggacc atcagccgcg cccggttggc aaagcgtttg accccgcgcg
     4981 gtgtcgggtc gtattgcacc agcgccgggt gccacaggcg cagcgcctcg acgaaggccg
     5041 cgctgtcgaa ggtgcgctcc gcgcggccca gcgcccggtt cacgctgctc gccacgccgg
     5101 ggaacaggcg ctgcgccagg atcagcagca aggtcccgat cagcgcgcca ccggcaattc
     5161 caaaggtgac cgactccggc acgcgcgtcg cggtactgag catgggcgca ccggcgtcga
     5221 ccgatggagt cttgggcgtt ggtggcggat tggtggcggc cgacgcggcc ggactggcgg
     5281 caaccgccgt ctgactttgc acagccggta tgtcgagcgg cagttgagcc acgccgagcg
     5341 tggcattcat ccagtcgaag gcaggcagcg cgagcaggac aacgccgatg accgccggcc
     5401 cgcccagcag cagacgcagc ggcagccagc gccccagcag acgcagcagc cggcccagcc
     5461 agcccggcga atcgcctgcc ggcgggtcgt acaggcggtc ggtctgcgcc atgcggtggg
     5521 gcaccggcac ctccacatgg atcagcttgc gcaggtagcg gcgggcgtac tcagtgttgt
     5581 ctgtcccttc gcggttggag gcgatgctgc cttcgatgtg ttcgatggcc acccccagca
     5641 cgatgaaaca ctggccgatg tcggtcaggt agttggtcag ttcgagcagg ctgttcacgg
     5701 tcgacgcact gcaccggtcg aggtcgtcga cgaacagcac caggcggcca tcgagcgcct
     5761 gcgtcagcag ggcgaactcg gtgcggtagt gttcgatcag ccccgtgggc tctttttcgt
     5821 ccgccgactg cccggtcaga cgattcacca ggccattcaa gggccgcagc aactggaagc
     5881 cgtagaccga cacgcccttg gtcagcagca gcgcgagcag cggccccagc gacagcagca
     5941 gccactccat ccgcggcagc aagggtggag cgggcagagt cgttgccact ttgagcagat
     6001 gctggcgttc atcgaccgtc agcgtgaacc cggcgtgctg gtcgatggtg gcgaacagct
     6061 gctgggagtg gtcgaacacg caacgcagcg ccggaggcag cggacgtggg tcgcggcaac
     6121 cggtgtcggc atcgccacca ccgccgccgt acaccagcga cgtgcggagg tagtcgatca
     6181 gcgcttgccg cacccgcagg ctgtcggcgg ccgccgtctc ggcaggcgcg ggaggtggct
     6241 tgactgtttt gttgtcgggt ggagcggatt tcgatttatc ggccggcgtt gcggtgggcg
     6301 caggagtgcc cttggcggtg ttgttgtcgg tcggagctgg gcttgatgga gcgaccggcg
     6361 tcagccgcgc cagtgactcc ggcgtcagcc gcacccgttg ttcgcccagc aggtggtggc
     6421 tggccgacca gcgcagccgc tcgccgcccg acagcacaat gccctcgtgg gtgtagtcgt
     6481 gcacaacatc ctgcccggtc agcagcaggc caaacagcat caccggcagg gtgaaccgat
     6541 aggccccgcc gcggccccac agcagccgcc agcgcacccg cagcgcggcg atggcctcgc
     6601 ggctgaacag actcggcatc gcctgcctgc ggatcacgtt gaacagcgcg ctcatcggcc
     6661 ggccctcctg ctggtggtgc caggcgttga accacgcagt gcgaaagccg gcctgctcca
     6721 ggtcgcgctt caacatgcgc atcaccgacg acttgccctg cccccactca ccggtgatcg
     6781 ccagcgtcag gtgcggttcg gtgcccgcat tgcggatgaa gcccgacagc gtctgcaccg
     6841 tcggccggta gttcagccgg tcctgcgcc
//
LOCUS       DAORCQ010000114         6759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_8057_length_6759_cov_9.953461, whole genome shotgun sequence.
ACCESSION   DAORCQ010000114 DAORCQ010000000
VERSION     DAORCQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_6759_cov_9.953461"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2684
                     /locus_tag="PKI23_10155"
     CDS             <1..2684
                     /locus_tag="PKI23_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37338.1"
                     /translation="LISAVDQTKTAFDSIKRGLGGLTDTAKSVNGVLANLGVAVSVAG
                     LTAMVKSAIDTGDALDEMSQRVGVSVETLSVWKPAAEQSGVSGESFEKGLRKLSTTML
                     EAATGSEDAARGFSAVGVEFKNQDGTLRATDQVLLDLAERFKAMPDGAEKTALAVQLF
                     GKSGAELIPFLNQGRDGINELAAEMQALGVQMSSETAAQAGNFNDALDKLKLATTSIG
                     NQIIASLLPALNDMAGGMVESAKQGGTLRVILDGVVLVLKTLALGAATVGKAFVALGE
                     AIGAGVAAAVEALKGNTEGAKAIIADLKGNLVKRLDELASFRDSLFDPKPIEVKAPKI
                     QADPELLQRLTKPKAVKAAQDTTGAQTTLMKAQLDAEFALLKDGLNRQQTALDAALED
                     RLVSVRDYYTQRTAIEQREVDAEIARKQQELARSQQVAATGKSENDRLKAKAEVAKAE
                     ADLITLNNRRTDIEQANARKAAQAERELADALAQAREELAQITGTATDADRQAAIERS
                     YRDLRARLAAESDADGVSLVDRLINVKAAQANLAALEAQWRQVTERLRNAQEAIQTQQ
                     QAGLLTEAQARQQIVALQQQSATEMERLLPTMQQAAQAIGPDAVIRVQAWRNELDRTK
                     LTVDEMAPLWNRIGESFGGALNGMITGAQTWRSALASIFQQVADAFLQQIVIQPFQQW
                     IAMQARMLALKLGFIQQEQTVDAAASAAKVAQKTTETTAVVSMDAAKAGAGAAASQAS
                     IPYVGPALAVAAMVAMVAAVMALLGGIKKFAGGGLVSGPGSATSDSIPARLSAGEYVV
                     RAAAVRQVGVAFLDSLNGLSAGPRFKGGELAFAAGGLVPEVKVPPAQPQMNQAVRIVN
                     AVDPGVTHDHLQSPAGEKVIVNIIGRNARAIRAALQG"
     gene            2704..3762
                     /locus_tag="PKI23_10160"
     CDS             2704..3762
                     /locus_tag="PKI23_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37339.1"
                     /translation="MALLFIDGFDHYDPQAVDSFGDPWLARGKAAYLSPQATRINGRR
                     PSSYALRLPEGSGGGYVKNLDATKTSLIVGAAVRVVPYQNTYTEPLLLGVRDANSQVA
                     HLVKIGEDGRLKLYRWQYGYDQLISVSVASAPARGWHYIELQVTQGTSNGVLSVRING
                     ILAIQMTAQNTIQGGGQLLTAFVGAVPGQNCPLTIDVDDFYIADTSGTINNTFLGDVR
                     VDALQAQADGSLNQWTPSPVGTAAWEAVSDEDEATAISAPNVGLRQSFDVEPLPVMAT
                     PAIYGVQLTMLARKTDAGLGKVKGLVVSGAQSAVSTDIVLQEQLAWQSTLFERNPNGN
                     VQWTEAAFNAAEFGVESA"
     gene            3759..5342
                     /locus_tag="PKI23_10165"
     CDS             3759..5342
                     /locus_tag="PKI23_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37340.1"
                     /translation="MTDRVVVQDLAEVSSKPTPGSELPAFQGEVLSRATFGASAASFT
                     PETAVAPLPPNLAASLLAESLAGPWPPIDAPIFLVEVLRRDTASSAIVATGMDAFGDQ
                     PWPDAQRGVFAFRHDWMEPLVERLEWQTSVTRLASGNESRQARRRVPRRWLTYKVGNA
                     RQTDALVADWLADHLGQMALWPLPQYAVHLTESCERGALALNVTEADGRQFGPLSANV
                     HLTYDGVQGWQETENNGRWILIIAADGWQIAQLSDVESDLLWLTEPLARAAAVGSTIM
                     PLVWGKAIDPADLTQWVPGMVGGNIPTQIQPAPLPDQDVLDDPWLDEIPVWPDGNWRD
                     DPTAAAQATITRQDLSPADPWVRRDDPWATTTLQRRYLASSLDEIEIWRARLWRAQGR
                     LEAFWLPDGLAPILWVTVEADPEDGFLRVDGKDISARISDFWHRPAACLIVHPDGYRQ
                     YALTATCHLDQGGVLVLRSGLDDWVPAGSRVIRLVRCRLDHDAIDLYWHSPTLLEITL
                     TARQLPEPRGNDRQTYEGE"
     gene            5348..6136
                     /locus_tag="PKI23_10170"
     CDS             5348..6136
                     /locus_tag="PKI23_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HNN37341.1"
                     /translation="MSQNPLLEVELYAFASNSAQFYLTPHEFDVDLDGNLYKSLALER
                     NELALGAEAAKAGLDLKLPPNCDLVRHLLANSLTGDTTSITLRIGRRDTWGDYWWISG
                     TRWMGRVLGVEVADDVARVRCESAQVSLKRIGLRRLYSRKCSHVLYSAACGASPISAS
                     ALVSNSNGRNVDLDGGTPGSVSGGLAGGWLQTPEGARHMIVSDYGGGVELLYPVAIEV
                     GTEVLLTVGCDHSTATCESRFGNLDNYGGFPAIPSKNPFSTGVF"
     gene            6155..6382
                     /locus_tag="PKI23_10175"
     CDS             6155..6382
                     /locus_tag="PKI23_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37342.1"
                     /translation="MWYLVVIVVAALVSVALAPKPPEPKPASLSDVDAPTAEEGRPIP
                     VVFGTVLLRGSNVVWYGDLEADPIKKKGGKK"
     gene            6379..6594
                     /locus_tag="PKI23_10180"
     CDS             6379..6594
                     /locus_tag="PKI23_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37343.1"
                     /translation="MTTQTVITIDHVRAVGLCVNGTRTWFARHDLDFRAFLRDGCDAD
                     TLLATGDAMAQRVVEHARNQSSQREQG"
BASE COUNT         1255 a         2151 c         2217 g         1136 t
ORIGIN      
        1 tcctgatcag tgccgtcgac cagaccaaga ccgctttcga ctcgatcaag cggggtctgg
       61 gtggcctgac cgacaccgcc aagagcgtca acggcgtgct ggccaacctc ggggtggctg
      121 tctccgtggc cggtctgacc gcgatggtca aatcggccat cgacactggt gatgcgctgg
      181 acgagatgtc gcaacgtgtc ggtgtcagcg tcgagaccct gtcggtatgg aaaccggcag
      241 ccgagcagtc cggtgtgtcc ggcgaatcgt tcgagaaggg gctgcgcaag ctgtccacca
      301 cgatgctgga agccgcgacc ggatcggaag atgccgcgcg cggattctcc gccgtaggtg
      361 tcgagttcaa gaaccaggac ggcaccctgc gcgccaccga tcaggtgcta ctcgatctgg
      421 ccgagcgctt caaggccatg cccgatggcg cggaaaaaac cgcgctggca gtgcaactgt
      481 tcggcaagtc gggagccgag ctgatcccgt tcctgaatca ggggcgcgac ggcatcaacg
      541 agttggctgc cgagatgcag gcgctcggcg tgcagatgag cagcgaaacg gcggcgcagg
      601 cgggcaactt caacgatgcg ctcgacaagc tgaaactggc caccaccagc atcggcaacc
      661 agatcatcgc gtccttgctg cccgccctga acgatatggc cggaggcatg gtcgagtcgg
      721 ccaagcaagg cggcacgctg cgcgtgatcc tggatggcgt ggtgctggtg ctcaagaccc
      781 tggccctcgg tgccgccacc gtcggcaagg ccttcgtcgc cttgggcgag gccattggcg
      841 caggtgtcgc ggcggcggtc gaggcgctca aggggaacac cgaaggggcc aaggccatca
      901 ttgccgacct caaaggcaat ctggtcaaac ggctggatga actggcgtcc ttccgtgaca
      961 gcctgttcga ccccaagccc atcgaggtca aggcacccaa gatccaggcc gatccggaac
     1021 tgcttcagcg cctgactaag cccaaagcag tcaaggcagc gcaggacacg accggcgcgc
     1081 agaccacgct gatgaaagcg cagctggacg ccgagttcgc gctgctcaag gacggtctga
     1141 accggcaaca aactgcgctg gatgctgcgc tcgaagaccg tctggtatcg gtgcgcgact
     1201 actacacgca gagaacggcc atcgaacagc gcgaggttga tgccgagatt gcccgcaagc
     1261 agcaggagct ggcccgcagt cagcaagtcg ccgccacggg caaatcggaa aacgaccgac
     1321 tgaaagccaa ggccgaggtc gccaaggcgg aagccgacct gatcacgctc aacaaccggc
     1381 gcacggacat cgagcaggcc aatgcgcgca aggcagcgca agccgagcgt gagctggccg
     1441 atgccttggc gcaggcgcgt gaggaactgg cccagatcac cggcaccgcc acggatgccg
     1501 accggcaagc agccattgag cgcagctacc gcgatctacg ggcgcgactg gcggcagaaa
     1561 gcgatgccga cggcgtgtcg ctcgttgatc ggctgatcaa tgtgaaggct gcacaggcca
     1621 atctggctgc gcttgaagct cagtggcggc aagtcactga gcgtctgcgt aatgcgcagg
     1681 aggccattca gacccagcag caggctgggc tgctaaccga agcacaggcg cgtcagcaga
     1741 tcgtggccct gcaacagcaa tcggccaccg agatggagcg cctgttgccg accatgcagc
     1801 aagccgcgca ggccatcggg ccggatgcgg tgattcgcgt gcaggcgtgg cgcaacgagc
     1861 tggatcgcac caagctcacg gtcgatgaaa tggccccgct gtggaatcgc atcggcgaga
     1921 gcttcggcgg tgcgctcaac gggatgatca ccggcgcgca gacctggcgc agtgccttgg
     1981 cgagcatctt ccagcaggtg gccgatgcct tcctgcagca gatcgtgatc cagccgttcc
     2041 agcagtggat cgccatgcag gcgcggatgc tggcgctcaa gctcggtttc atccagcagg
     2101 agcaaaccgt cgatgcggcg gccagcgccg ccaaggtcgc ccaaaagacc accgaaacca
     2161 ccgccgtggt gtcgatggat gcggccaagg cgggagctgg ggcggcggcg tcgcaggctt
     2221 ccattcccta cgttggcccg gcactcgcgg tggccgcgat ggtagccatg gtcgccgctg
     2281 tgatggcgct cttgggtggc atcaagaagt tcgcgggtgg cggtctggtc tccgggccgg
     2341 gcagcgccac ctcggattcg atcccggcgc gtctgtccgc aggcgagtac gtggtgcggg
     2401 cggccgccgt gcgccaagtc ggcgtggcct tcctcgactc gctcaacggc ttgtcggccg
     2461 gcccacgttt caagggtggc gagctggcct ttgcagcggg cgggctggta ccggaggtga
     2521 aagtgccgcc cgcgcagccg cagatgaatc aggccgtgcg catcgtcaac gcggtcgatc
     2581 cgggtgtgac ccacgaccac ctgcagtcgc ctgccggaga gaaagtcatc gtcaacatca
     2641 tcgggcgcaa tgcacgggcc atccgtgcgg cgctgcaagg ctgaattttc aggggaaagt
     2701 ccaatggcac ttctgttcat cgacggtttc gatcactacg acccgcaggc cgtggacagc
     2761 tttggcgatc cgtggctcgc acgtggcaag gcggcgtatc tgtcaccgca ggccacccgg
     2821 atcaatggcc gtcgtccgtc ctcctatgcc ctgcgtttgc cggaaggttc gggtggcggc
     2881 tacgtcaaga acctcgacgc caccaagacc agcctgatcg tcggggcagc cgttcgcgtg
     2941 gtgccgtacc agaacaccta caccgagccg ctgctgctgg gcgtgcgcga tgccaactcg
     3001 caggtcgcgc atctcgtgaa aatcggcgag gacggtcggc tcaagctcta ccgctggcaa
     3061 tacggctatg accagttgat ctctgtctca gtcgccagcg ctccggcgcg cggctggcac
     3121 tacatcgagt tgcaggtcac gcaaggcacc agcaacggtg tgctgtcagt gcgcatcaac
     3181 ggcatcctgg ccatccagat gaccgcgcag aacaccattc agggcggcgg ccaactgctc
     3241 acggcattcg taggtgcggt gcccggccag aattgtccgc tgaccatcga cgtcgacgac
     3301 ttctacatcg ccgacaccag cggcacgatc aacaacacct tcctcggtga tgtgcgcgtc
     3361 gatgccttgc aggctcaggc cgatggcagt ttgaaccagt ggactcccag tccggtcggc
     3421 actgctgcct gggaagccgt cagcgacgag gacgaagcca cagcgatcag tgcgccgaac
     3481 gtggggctgc gccagtcctt cgatgtcgag ccgctgccgg taatggccac gcccgccatc
     3541 tacggcgtcc agctcacgat gctggcgcgc aagaccgacg ccggtctggg caaggtcaaa
     3601 ggcctcgtgg tcagtggtgc gcagagcgct gtcagcaccg acatcgtcct gcaggagcaa
     3661 ctggcgtggc agagcacgct gttcgagcgc aatccgaacg gcaacgtgca gtggacggag
     3721 gccgccttca atgccgctga gttcggcgtg gagtcggcat gacggatcgc gtcgtcgttc
     3781 aagacctcgc ggaggtttct agcaagccga cgccggggag cgaactgccc gccttccagg
     3841 gtgaagtgct ctcgcgcgcc accttcgggg cgagcgcagc cagcttcacg ccggaaacag
     3901 ctgtcgctcc gctgccgccc aatctggcgg ccagcctgct ggcggaatcc ttggcgggcc
     3961 cctggccacc catcgatgca ccgatctttc tggtcgaagt gttgcgccgg gacacggcct
     4021 caagcgccat cgtcgccacc ggtatggatg cctttggcga ccagccttgg ccggatgcgc
     4081 aacgcggcgt gtttgccttc cgtcatgatt ggatggagcc cctcgtcgaa cggctggagt
     4141 ggcagaccag cgtcacgcgg ctggccagtg gcaacgaatc ccggcaggca cgccgacgcg
     4201 ttcctcggcg ctggctcacc tacaaggtgg gcaacgcccg gcagaccgat gccctggtgg
     4261 ccgactggct ggccgatcat ctcggtcaaa tggcgctgtg gccgctgccg cagtacgcgg
     4321 tgcacctgac cgagtcctgc gaacgtggcg cactggcact caatgtgacg gaggctgacg
     4381 ggcgacagtt cgggccactc tcggccaatg tgcatctgac ctacgacggg gtgcagggct
     4441 ggcaggaaac tgagaacaat ggccgctgga ttttgatcat cgccgccgat ggctggcaga
     4501 tcgcccaact cagcgatgtg gaaagcgatc tgctgtggct gacggagccc ttggcacgcg
     4561 ccgcagccgt gggcagcacc atcatgccct tggtgtgggg caaggccatc gatccggcgg
     4621 atctcacgca gtgggtaccc ggcatggtcg gcggcaacat tcccacacag atccagcctg
     4681 cgccactgcc cgaccaggat gttctcgatg acccatggct cgacgagatc ccggtctggc
     4741 cagatggcaa ctggcgtgac gatccgacag ccgccgcgca agccacgatc acccgccagg
     4801 atttatcccc tgcagatccg tgggtgcgcc gggacgatcc gtgggcgacg acaactttgc
     4861 agcggcgcta tctggccagc tcactcgatg aaatcgagat ctggcgggcg cggttgtggc
     4921 gcgcccaagg ccgtttggaa gccttctggc tgcccgatgg cttggctccg atcctgtggg
     4981 tgaccgtcga agccgatccc gaagatggct tcctgcgcgt ggatggcaaa gacatctccg
     5041 cgcgaatttc ggatttttgg catcgccccg ccgcatgttt gatcgtgcat ccagacggct
     5101 atcggcaata cgccctgacg gcgacctgcc atctggatca aggcggtgtg ctggtgctgc
     5161 gctcgggtct cgacgactgg gtgcccgcag gcagccgcgt cattcgcctc gtgcgctgcc
     5221 gcctcgacca cgacgccatc gatctgtact ggcacagccc gacgctgctg gagatcaccc
     5281 tgacagcgcg tcagttgccc gaaccacgcg gaaatgaccg tcaaacctac gagggagagt
     5341 aagcacgatg agccagaacc cattgctgga agtcgagcta tacgccttcg ccagcaacag
     5401 cgcgcagttc tatctgacgc cgcacgaatt cgatgttgat ctggatggca acctctacaa
     5461 gagcctggcc ttggaacgca acgaactggc gctgggtgct gaagctgcga aggctggctt
     5521 ggatctgaaa ctgccgccga actgtgattt ggtgcgccac ctgctcgcca actcgctgac
     5581 cggcgacacc acctcgatca ccctgcgcat cggacggcgc gacacctggg gcgactactg
     5641 gtggatctcc ggcacgcgct ggatgggccg ggtgctgggc gtcgaagttg ctgacgatgt
     5701 ggctcgcgtt cgctgcgaat cggcgcaagt cagtctcaag cgcatcgggt tgcggcggct
     5761 ctacagccgc aagtgttccc acgtgctgta ttcggctgcc tgtggtgcct caccgatttc
     5821 tgccagcgcc ttggtgagca acagcaatgg ccgcaacgtc gatctcgacg gtggcacgcc
     5881 cggcagcgtc agtggtggct tggccggtgg ctggctgcaa accccggaag gtgcccgcca
     5941 catgatcgtc agcgactacg gcggcggcgt ggagttgctc tacccggtgg ccattgaggt
     6001 cggcaccgag gtgctgctga cggtgggctg cgaccacagc acggctacgt gcgagtcgcg
     6061 cttcggcaac ctcgacaact acggcggctt tcccgccatc ccgagcaaaa acccgttctc
     6121 gacgggcgtg ttctgaatcc ctggagaaat cgccatgtgg tacctcgtcg tcatcgtggt
     6181 ggcggcgctg gtttcggtcg cgctcgcgcc gaaaccgccc gaacccaaac cggcgtccct
     6241 gtctgacgtc gatgccccca ccgcagaaga aggccgaccg attcccgtcg tgttcggcac
     6301 cgtgctgctg cgtggctcca acgtcgtctg gtatggcgat ctggaagccg acccgatcaa
     6361 gaagaaaggt ggcaagaaat gactacgcag accgtcatca ccatcgatca cgtgcgcgcc
     6421 gtgggcctgt gcgtgaacgg cacgcgtacg tggtttgcgc gtcacgacct ggatttccgg
     6481 gccttcctgc gcgatggctg tgacgccgac accttgctgg ccaccggcga tgcaatggcc
     6541 caacgtgtgg tcgaacacgc ccgcaatcaa tccagccagc gggagcaagg ctgatgggtg
     6601 gcagcagcaa gaagcaaacc gtcggctatc gctatcggat gggactccat ctggccctgt
     6661 gccaggggcc cgtcgatgcc gtgcaggaaa tccagatggg cgaccgtacc gcgtggggtg
     6721 atgccgaccg cgcgccgctg tccagcgggc atgggctga
//
LOCUS       DAORCQ010000115         6752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_8072_length_6752_cov_5.782739, whole genome shotgun sequence.
ACCESSION   DAORCQ010000115 DAORCQ010000000
VERSION     DAORCQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8072_length_6752_cov_5.782739"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKI23_10185"
     CDS             complement(<1..850)
                     /locus_tag="PKI23_10185"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HNN37344.1"
                     /translation="MAESSSWRWPLSPPLTLLRCNVSKLVQQKDGALIQAERNVAAAL
                     SPTSFGWLLTLSLALLLPQLAQMPPLLLAVCAAALTIRSLWWRRRPDAVPLWLRLPLL
                     LSGLAAIYATYAGVVGVEPAVALLLLSFAGKWLELNSRRDGQVLILLGCFVMLAQFLF
                     DQSLLMAGYALFELLLIVTSWLVLLHGQQPMRALGWAARLLGQSLPLMVALFLLFPRI
                     GPLWSIPLSSSTARSGFAEEIGPGDISRLRLTDELVLRARFEGEIPPAAELFWRGLVL
                     AHFDGRN"
     gene            complement(964..3297)
                     /locus_tag="PKI23_10190"
     CDS             complement(964..3297)
                     /locus_tag="PKI23_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNN37345.1"
                     /translation="MKVSNKNIDHVPFLERLVFENRIAILVLVLLITGFLGYQMSQVR
                     PDVQFEKLIPLQHPYIQNSMKYMPTGGNAGNAVQIAVEVVDGDIFTKEYLETLRQIAD
                     DVFYTPGVDKGNMISMWSAGLRWEAATPVGWKGDAIIGSGYDGTPEAIEKVRLNVLRS
                     RHIGTYIANNFKATIIKVPLVGVDRSKGERFDYLAFSKKLEQDIRSKYQTDQIKIHIV
                     GFPKWIADLISGFQLIGLFFVISIGIAFVLLYIYSHCWKSSVVPLLCSIIAVVWQLGL
                     VKLLGYGLDPYSVLVPFLIFAIGVSHGVQIISGISSEAGQGEDKLMAARRAFRVLHIP
                     GAVAILCDVVGFSTLYMIPIKVIQELAVTASLGVFVILFTNLVLLPLLMSYIGVTKAS
                     IKHAQSQLNKDTPVWNAMSRFASGRIAPVTLLISAVLLGGAFYFRADLKIGDLDAGQP
                     MLRPDSVYNKDNAYVNANFSTSSDVMVVFLKTAPDKCTEYSSAVLIDQLTWALENDPG
                     VQSVFSLVTKAKYYSFNTTEANPKWSILARDQNLLTTQIGITGMNMDCSVAYVQVSLY
                     DHKAETLQRVAELVEKFAAENNSEEKQLLLAGGNAGIDAATNQEIKYSQNRMLLLVYA
                     AVTVLCLITFRSILATICIITPLIITSILCEALMAIMGIGFKVATLPVVALGVGIGVD
                     YGIYIFAKLESFLSEGYDLVEAYLHTLRTTGKAVFFTGFTLALGVGTWVFSPIKFQAD
                     MGILLVFMFLWNMVGAVWMLPAIAHYLIKPAKYAAKQ"
     gene            complement(3328..4446)
                     /locus_tag="PKI23_10195"
     CDS             complement(3328..4446)
                     /locus_tag="PKI23_10195"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNN37346.1"
                     /translation="MSLCALAMGFALHAHAVVDPIEAPAVPMPKAAHTLLLGVAKAGT
                     RLVAVGQYGIVVYSDDQGKNWQQAKVPVQLTLTDLFFVDDKTGWAVGDAAVILKTVDG
                     GATWEKQHDGLMPNSVASQLVAEGQKKIDEAQAALDQADPNDEAARAELDERLFQAED
                     RIQALKKASNSPLLNVWFRDQSEGFAVGSYAQFYHTADGGKTWESWAHKLQNAENHNY
                     DIWGDGSGLMLIVGETGNIFRSTDHGATWQTIPKVSPRSLYSIVPDVARKRLYITGMV
                     GQVAYSDDQGGSWTAMPTHTEFSVQNGLIGADGKPVFIGNNGLFITEKSDGSGLGVYL
                     RDDRVHMANIIDAGDGNFLTVGFAGVKRISPKGLDLPH"
     gene            complement(4777..5142)
                     /locus_tag="PKI23_10200"
     CDS             complement(4777..5142)
                     /locus_tag="PKI23_10200"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNN37347.1"
                     /translation="MSTVESFRRRLEPLLRIAFGLLFLLVGWLALTPQPATLSSGNDK
                     SDHLLAFLALTLLLRHGWSRRLAGQGVLAVMLGYGAAIELLQLRVPGRDGSLLDLGAD
                     LIGILLALGISRGGWVSLR"
     gene            5242..5652
                     /locus_tag="PKI23_10205"
     CDS             5242..5652
                     /locus_tag="PKI23_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37348.1"
                     /translation="MSRTSGMPDLGMDANTLYLEETFTDQRVGTLRRLTPVTAAGQPD
                     GSRPVLFQGQTQLMTQVGPLPLDFNIEASSLDEAVARFGSSAQQALEKTLRDLEEMRR
                     ESASSLIIPGGGGPSGGPLGGGPLGGGRGGKILR"
     gene            5681..>6752
                     /locus_tag="PKI23_10210"
     CDS             5681..>6752
                     /locus_tag="PKI23_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNN37349.1"
                     /translation="MKSRAAIALAPGRPLELVEIDVAEPRAGEVLIRNIATGVCHTDA
                     YTLSGRDPEGLFPVILGHEGGAIVEAVGSGVRNVAVGDHVIPLYTPECGECKFCRSGK
                     SNLCVAIRASQGKGVMPDGSSRLSFEGRMVHHYMGTSTFSEYGVLPEIALARVPKQAP
                     LDKVCLLGCGITTGVGAVRHTARVEAGATVAVFGLGAVGLSVIQGARMANAGRIIAID
                     INPTKFDFARQLGATDCVNPAEHGLPLQQVIIDLTDGGVDYSFECVGNVDLMRVALEC
                     CHRGWGVSTIIGVAGGGEEIRTRPFQLVTGRRWQGSAFGGVKGRSQLPDYVQMYLDGK
                     LEVDRFITHTLPLSDINRAFELM"
BASE COUNT         1365 a         2157 c         2016 g         1214 t
ORIGIN      
        1 agttgcgtcc atcgaagtgg gccagcacca gaccgcgcca gaacagctcg gcggcaggcg
       61 gaatttcccc ttcgaaacgg gcccgcagca ccagttcatc cgtcagccgc aaccggctga
      121 tgtcacccgg gccgatctct tcggcaaagc cgctgcgggc agtcgaactg gagagcggaa
      181 tcgaccagag cggcccgatg cgcggaaaaa gcagaaaaag cgccaccatc aacggcagac
      241 tctgccccag cagccgcgcg gcccaaccga gcgcccgcat cggttgctgc ccatgcaaca
      301 gcaccagcca actggtgacg atcagcagca gctcgaacag cgcgtagccc gccatcaaca
      361 ggctttggtc gaacagaaac tgtgccagca tgacaaagca gccgagcagg atcagcacct
      421 gcccatcacg ccgactgttc agctccagcc acttgccggc aaacgacagc agcaacagcg
      481 ccaccgccgg ctccaccccc accacaccgg cataggtggc atagatggcg gccaatcccg
      541 acagcagcag cggcagccgc agccagagcg ggaccgcatc aggtcgacga cgccaccaga
      601 gcgaccggat cgtcaacgcg gccgcgcaga ccgccagcaa cagcggtggc atctgcgcca
      661 gttgcggcaa cagcagcgcc aacgagagcg tcaacagcca gccgaatgac gtgggcgaga
      721 gcgctgcggc gacattccgc tcggcctgga tcaatgcacc atccttttgt tgcaccagct
      781 tggagacatt gcagcgcaaa agcgtcagcg gtggcgaaag cggccaccgc cacgacgaag
      841 attcagccac tccctgctcg gcatgacccg aggaatgaaa aaccccggca caggccgggg
      901 ttttttactc aatcggccat tacatcagca atggccggac acacgccgct tgccgaacga
      961 aactcactgt ttcgctgcat attttgctgg cttgatcagg tagtgcgcga tggcgggaag
     1021 catccagacc gccccgacca tgttccacag gaacatgaag accagcagga tgcccatgtc
     1081 ggcctggaac ttgatgggcg agaagaccca ggtgccgacc ccgagcgcca gcgtgaagcc
     1141 ggtgaagaac accgccttgc cggtggttct cagagtgtgc aggtaggcct cgaccaggtc
     1201 atacccttca ctgagaaagg attccagctt ggcgaagatg tagattccgt agtcgacgcc
     1261 aatgccgacc cccagcgcca ccaccggcag cgtcgccacc ttgaagccga tgcccatgat
     1321 cgccatcagc gcctcacaga gaatggaggt gatgatcagc ggcgtgatga tgcagatggt
     1381 cgccaggatc gaacggaagg tgatcagaca gagcacggtc accgctgcat agaccagcag
     1441 cagcatgcgg ttctgcgaat atttgatctc ctggttggtg gcggcatcga tgccggcatt
     1501 gccaccggcc agcagcagct gcttctcctc gctgttgttt tcggccgcga acttctcgac
     1561 cagctcggca acccgctgca acgtctcggc cttgtggtca tagagcgaca cctgaacata
     1621 ggcgacgctg caatccatgt tcatgccagt gatgccgatc tgcgtggtca gcaggttctg
     1681 atcacgcgcc agaatcgacc atttcgggtt ggcctcggtg gtgttgaagc tgtagtactt
     1741 cgccttggtc accagcgaga agaccgactg tacgcctggg tcattctcca gtgcccaggt
     1801 cagctgatcg atcagcaccg ccgaggagta ctcggtacat ttgtccggtg cggtcttcaa
     1861 aaacaccacc atcacatcgg agctggtgga gaagttcgcg ttgacgtagg cattgtcctt
     1921 gttgtagacc gaatcgggtc gcagcatcgg ctgcccggca tccaggtcac cgatcttgag
     1981 gtcagcgcgg aaatagaatg cgccacccag caataccgcg gagatcagca gggtcacagg
     2041 tgcaatcctg ccgctggcaa agcgcgacat cgcattccat accggcgtgt ccttgttcaa
     2101 ctgggactga gcatgtttga tgctggcctt ggtgacaccg atgtaggaca tcagcaacgg
     2161 cagcagcacc aggttggtga acaggatcac gaacaccccg aggctggcgg tgacggccag
     2221 ctcctggatc accttgatcg gaatcatgta gagggtggag aagcccacca cgtcacacag
     2281 aatcgccacc gcaccgggga tgtgcaacac acggaaggcg cgacgggcgg ccatcagctt
     2341 gtcctcgccc tgcccggcct cgctcgatat ccccgagatg atctgcacgc catggctgac
     2401 gccgatcgcg aagatcagga acggaaccag caccgaatag gggtcgaggc cataccccag
     2461 cagcttgacc agaccgagct gccagaccac ggcgatgatc gaacagagca gcggtacgac
     2521 cgagctcttc cagcagtgtg aatagatata gagcaggacg aaggcgatcc cgatcgagat
     2581 cacgaaaaac agcccaatga gctggaagcc cgagatcagg tcggcgatcc acttcggaaa
     2641 gccaacgata tggatcttga tctgatcggt ctgatacttg gaacggatgt cctgctcaag
     2701 cttcttggaa aacgccagat agtcgaaccg ttcacccttg ctgcggtcga ccccgaccag
     2761 cggcaccttg atgatggtcg ccttgaagtt gttggcgatg taggtgccga tgtggcgtga
     2821 ccgcaggaca ttcaaacgca ccttctcgat cgcctcgggc gtgccatcgt agccgctgcc
     2881 gatgatggca tcccccttcc agccgaccgg cgtggccgcc tcccagcgca ggccggccga
     2941 ccacatggag atcatgttgc ccttgtcgac gcccggtgtg tagaagacgt catcggcgat
     3001 ctgtcgcaag gtctccagat actccttggt gaagatgtca ccatcgacca cttcgaccgc
     3061 gatctgcacc gcgttccctg cgttgccgcc ggtcggcatg tacttcatcg agttctggat
     3121 gtagggatgc tgcagcggaa tcagcttctc gaactgcaca tcagggcgaa cctgagacat
     3181 ctggtaaccc agaaagccgg tgatcagcag caccagcacc agaatcgcaa tgcggttctc
     3241 gaagaccagc cgctccagaa atggaacgtg atcaatgttt ttattggaga ctttcatgtt
     3301 tgaatcccgc tttatgcctc agatcaatca gtgtgggagg tcgagccctt tgggggagat
     3361 gcgcttgacg ccggcaaaac caaccgtcaa gaaattgccg tccccggcat cgatgatgtt
     3421 ggccatgtgc acccggtcat cacgcaggta gacgccaaga ccactgccat cgctcttctc
     3481 ggtgatgaac agaccgttgt tgccgatgaa aaccggttta ccatcagccc cgatcaggcc
     3541 attctgtacc gaaaactcgg tgtgggtcgg catcgccgtc cagctgccgc cctgatcatc
     3601 gctgtaggcc acctggccga ccatgccagt gatgtagagc cgcttgcgcg cgacatcggg
     3661 cacgatggag tagaggctgc gcgggctcac cttgggaatg gtctgccaag tggcgccatg
     3721 gtcggtggag cggaagatgt tgccggtctc gccaacgatc agcatgaggc ctgagccatc
     3781 gccccagatg tcgtagttgt ggttttcggc attctgcagc ttgtgcgccc agctctccca
     3841 cgtcttgccg ccatcggcgg tgtggtagaa ctgcgcatag gagccgaccg cgaaaccctc
     3901 gctctggtca cggaaccaga cattgagcag cggtgagttg ctggcctttt tcagcgcctg
     3961 gatgcggtcc tcggcctgga acagccgctc atccagttcg gcacgggccg cttcgtcatt
     4021 ggggtcggcc tggtcgagtg cggcctgcgc ctcgtcgatc ttcttctgcc cttcggcaac
     4081 cagctggctg gccacgctgt ttggcatcag gccatcgtgc tgcttctccc aggtggcacc
     4141 gccatcgacg gttttcagga tcaccgctgc gtcaccgaca gcccagccgg ttttgtcatc
     4201 gacaaagaac aggtcggtca gggtgagctg caccggcacc ttggcctgct gccagttttt
     4261 gccttggtca tcggaataga ccacgatgcc atactgaccc actgccacca gacgggtgcc
     4321 ggccttggcc acgccgagca gcagggtatg tgctgccttg ggcatcggca ccgccggtgc
     4381 ctcgatgggg tcgacgaccg catgggcgtg cagcgcaaac cccatggcca gcgcgcaaag
     4441 cgacatgcca aggcgccgaa gcgattgtgg acgcctgctc agccctgaga atctttggct
     4501 aatccatcgc cccgatgggt tgcgagcaca acccattcga tcgtttttgg cagatagcat
     4561 gaactccccc tagaagatgc tgtcgttatt atttcagcgc gccatgttaa ccgggcttgc
     4621 ggatggaatc taaccctctg ccattcccgc gacagagggt ttcgtttcta ccccggtttc
     4681 cagcacgaaa gcggcgactc cgcctgcgca ctgtttcatt gggtaacgaa acctgcattc
     4741 agtcacccgg cccaccggac aactgccaga catgcttcag cgaagcgaca cccatccacc
     4801 gcggctgatg cccaaagcca gcaggatacc gatcagatca gcccccaggt ccagcagcga
     4861 gccgtcccgt cccggcaccc gcagttgcag cagctcgatg gcagcgccat agccaagcat
     4921 cacggccaga acgccctgcc cagcaagacg ccttgaccag ccatgacgca acagcagggt
     4981 cagcgccaga aaagccagca ggtggtcgct cttgtcgttg ccggaggaga gcgttgccgg
     5041 ttgcggagtc agcgccagcc agccaaccag cagaaacagc aacccaaagg cgatccgcag
     5101 cagcggctcc agtcggcgcc tgaagctttc aacagttgac attgcgcact ccgctcctgg
     5161 ttcgagtcga tcactcgggt cgatttctgc taggctagcg ccgcttcgat ttctgtcact
     5221 tcattcgcca cgaggccttc tatgtcacgc acttccggca tgcccgatct cggcatggat
     5281 gccaacacgc tctatctcga agagaccttc accgaccagc gggtcggcac gctgcgccgt
     5341 ctgacgccgg tcactgcggc cggccagccc gacggcagcc gtccggtact gtttcagggg
     5401 cagacgcaac tgatgaccca ggtcggcccg ctgccgctcg attttaacat cgaggccagt
     5461 tcgctcgatg aagccgtcgc ccggttcggc agcagcgccc agcaggcgct ggagaagacc
     5521 ctgcgcgatc ttgaagagat gcgccgcgaa tcggcctcgt cgctgatcat ccccggcggt
     5581 ggcggcccca gcggtggccc gctggggggc ggtccgctcg gcggtggtcg gggcggcaag
     5641 attctgcgtt gatcatcagt gacgaaggag gatgcccaca atgaagagcc gtgccgccat
     5701 cgcgcttgcc ccgggacgcc cgctcgaact ggtcgaaatc gacgtggccg aaccgcgcgc
     5761 cggcgaggtg ctgattcgca acatcgccac cggcgtctgc cacaccgatg cctacaccct
     5821 gtcgggccgt gatcccgaag ggctgtttcc ggtcattctc ggccatgagg gcggcgccat
     5881 cgtcgaggcc gtcggcagcg gcgtccgcaa cgtggcagtg ggcgaccatg tcatcccgct
     5941 ctacaccccc gagtgcggcg agtgcaagtt ctgccgctcc ggcaagtcga acctctgtgt
     6001 cgccatccgt gccagccagg gcaagggggt gatgcccgat ggcagcagcc ggctgtcgtt
     6061 cgaaggcagg atggtccacc actacatggg cacctcgacc ttctcggaat atggcgtgct
     6121 gccggagatc gcgctggcca gggtgccgaa gcaggcgcca ctggacaagg tctgcctgct
     6181 cggctgcggc atcaccaccg gtgtcggcgc cgtgcgccac accgcccggg tcgaggccgg
     6241 cgcgaccgtg gcggtcttcg gcctcggcgc cgtcggactc agcgtgatcc agggcgcgcg
     6301 catggccaat gccggacgga tcatcgccat cgacatcaat ccgaccaagt tcgactttgc
     6361 ccgccagctg ggtgccaccg actgcgtcaa tcccgccgag catggcctgc cgttgcagca
     6421 ggtgatcatc gacctgaccg acggcggcgt cgattactcg ttcgagtgtg tcggcaatgt
     6481 cgatctgatg cgtgtcgcgc tggagtgctg tcaccgtggc tggggtgtct ccaccatcat
     6541 cggtgtcgcc gggggcggcg aggagattcg cacccgcccc tttcaactgg tcaccggccg
     6601 tcgctggcag gggagcgcgt tcggcggcgt caagggacgc agccagttgc cggactacgt
     6661 gcagatgtac ctcgacggca agctggaggt ggaccgcttc atcacccata cgctgccgct
     6721 gtccgacatc aaccgggcgt tcgaattgat gg
//
LOCUS       DAORCQ010000116         6550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_8418_length_6550_cov_5.955504, whole genome shotgun sequence.
ACCESSION   DAORCQ010000116 DAORCQ010000000
VERSION     DAORCQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8418_length_6550_cov_5.955504"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..579
                     /locus_tag="PKI23_10215"
     CDS             202..579
                     /locus_tag="PKI23_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37350.1"
                     /translation="MRSLQRTIIPLLLLSFVVVSLGAYSPGSRALLHQLDHERHLFGL
                     SGEHAHQLAPKADDPAKPQPLSDLEHRLLHALSHFEPVLDASVPMPAPLHADIIPSPP
                     RPLSLPPRVAESPFRPPRGLLQS"
     gene            678..1805
                     /locus_tag="PKI23_10220"
     CDS             678..1805
                     /locus_tag="PKI23_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNN37351.1"
                     /translation="MNNRIAGALPLRRWVALAICLSFATADAAEPPIRLTIADSQVQA
                     LGIQTQPLSPSSGAVTARFAAEVTLPPQAARRISAPLAGMVTELLVQPDQAVRAGDPL
                     LRLAGEQWGALQSALLQADARARLARQSLQREQALFAEGIIPQRRLQEAQTALAESEA
                     VLGQARMALQATGLPMALVEQLLSAAKPQQNLTLTAPTAGRVSQIEVQPGQQVEAADA
                     LLQLTQPDPLWLAIRMPVSEGDRWQPGTALTVAGKAVTARLLRAGTILAADSQTFVWH
                     ATVERGGEQLRPGEFVAVELAVAEGEQGWEVPLAAIARDGDQACLFVRTAEGFEARPV
                     RILASGGQQVRVQGLLQSGEQIAVSGVVALKGAWLDARGGE"
     gene            1805..4888
                     /locus_tag="PKI23_10225"
     CDS             1805..4888
                     /locus_tag="PKI23_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNN37352.1"
                     /translation="MLAPLIRFSLSQRLFILLATLLVAALGWMAFRGLPIDAFPDVSS
                     TQVKVIMKAPGMTPEEVESRIAVPIEVEMLGIPHSKILRSVTKYGLVDVTIDFEDGTD
                     IYWARQQVNERLAGLGESLPPGITGGMAPVTTPLGEMFMFTVEGEGLSLTERRSLLDW
                     VIRPALRTVPGVAEVNALGGAVRAFEVVPDPLKLAASGVTLGQLKETIQANNRNDGAG
                     RLGEGEEVLLVRSEGSVTTLEDLRSIVVARKAGVPVPLGALAEVRIGTMTRYGAVTRS
                     GQGEAVQGLVLGLRGANAQQVVEGVRQKIAELQPSLPPGVRIDTFYDRAALVTQAVGT
                     VSSALIEATLLVVVLLVLFLGDLRAALTVALVLPLAALITFILMRNFGMSANLMSLGG
                     LAIAIGMLVDAAVVVVENIVQRLATDPSGGRLPRLHIIYRAVREVVVPVTSGILIIVT
                     VFLPLLTLQGLEGKLFTPVALTIVFALLASLLLSLTVIPVLASYLLGEARHEEPWLPR
                     KLLTLYEPALAWGMRRQPLVAAVALLMLLLAALVYTQIGKSFMPTLDEGDLIVGIEKL
                     PSISLEQSVALDLKIQQAIMQAIPEVTGIVARAGADEIGLDPMGLNQTDTYLLLKPRD
                     EWNLPDKAALMEAVRKVLDPLPGIEYSFTQPIEMRVSEMVIGVRGDLAVKIFGPDLAR
                     LNDHAGQIETLLKRIPGNQDVYTVQNDGVQYLRVVIDRLQAGRLGLTVEEIQDALRIQ
                     IEGARAGVVIEGNQRTPIVLRGAEAVRLSPAEFAALRITTAEGATVPLASVARLERAA
                     GPVKIDREMGSRYSVVIANVSGRDLVSFVEEAKALIAQKVALPSGYRITWGGQFENQQ
                     RAAARLTVVVPVALALIFVLLFSTFGSVRQALLILSNIPFALVGGIVALWLTGEYLSV
                     PASVGFIALLGIAVLNGVVLVSHFNQLHGEGLPLAEVVVQGARRRLRPVLMTALITAF
                     GLIPLLFSTGPGSEIQRPLAIVVIGGLITATALTLMLLPILYLRFALPKPEVSDV"
     gene            4881..5183
                     /locus_tag="PKI23_10230"
     CDS             4881..5183
                     /locus_tag="PKI23_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HNN37353.1"
                     /translation="MSELCLTLLCPPQIAEALLDLLLTWPQNLLFTSTPMAAHGLTVD
                     VLDQSEQVMGRARAIAITVLLSDADKRTLLGTLHQQFAGSGLRYWLTAVTETGEIA"
     gene            5180..6424
                     /locus_tag="PKI23_10235"
     CDS             5180..6424
                     /locus_tag="PKI23_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNN37354.1"
                     /translation="MKAPLLGMVLTLIAPMIAAELAPPPMTGLLPTELARPLLDQDPG
                     VAAAQADLQVALHEAGILERSPHEWSLRTTGQQRNLDRGPTHDEWHLALERTLRLPGK
                     AAADRGLGAATVEAAQARRGELLHETARELMTLWLEWLAAEQTRELAADALLAVERAQ
                     AAVHKRVTAGDAAQLDLSLAQAELATQQVFSNDAQTQAAASWARLSTRFPATPRQPLP
                     MAEPIPVEGTVALWQRRILEQSDALKLTEAALQGDEARAARARSERLPDPTLGAFTAS
                     EASGHERIAGVSLSLPIPGGSRALRDAQAYAMVEVSRQRVELKRRQLESEIASAVITA
                     QGAYRSWQTAREGAAAMQQNAALVERAYTLGETDLQTLLLARRQAISARQGALQARTA
                     ALNSHYGLLIDAHLVWELAHEE"
BASE COUNT         1066 a         2115 c         2270 g         1099 t
ORIGIN      
        1 gtgctctttt catcacatcg actccttgaa aacggtgagt ggacatctca tccagttcga
       61 gacgatatga cagttcgaag cagggcgcag cagcgtgctt gaccccgcac tggcgaccgg
      121 ccgaaaatgg tactcgcacc ccttgcccgg gagcgttcag gttcattccc tctctatcga
      181 cgaaaccccg caagccaatc aatgcgcagc ctccagcgaa cgatcatccc cctgctgctg
      241 ctcagcttcg tggtggtgag cctgggcgcc tacagccctg gttccagggc gctgctgcat
      301 cagctcgacc atgagcgcca tctgttcggg ctctctggcg aacatgcgca ccagcttgcg
      361 ccgaaagcgg atgacccggc gaaaccgcag ccattgagtg atctggagca tcggctgctc
      421 catgcactga gccatttcga acccgtgctc gatgcgtcgg tgccgatgcc ggcaccgctc
      481 catgccgaca tcatcccttc accgcctcgc ccgctttcgc tgccacccag agtggccgag
      541 tcgccgttcc gcccaccgcg aggcctcctc cagagctgat tccgcccgcg cggagtcgat
      601 cttgccgagg accgtcgacc cggttgaatg ggtcgacggt gcttcgttgc gatccctgaa
      661 gccattcgag gtctctgatg aacaacagaa tcgctggtgc gctgccattg cgccgctggg
      721 tggcgctggc catctgtctc tcttttgcca cggccgatgc ggccgagcca ccgatccgcc
      781 tgaccatcgc cgacagccag gtgcaggcac tcggcatcca gacccagccg ctgtcgccat
      841 cgagcggcgc ggtgacggcc cgctttgcgg ccgaggtgac gctgccgcca caggctgcgc
      901 ggcggatcag cgcgccactg gcaggaatgg tcaccgagtt gctggtgcag ccagaccaag
      961 ccgtgcgtgc cggagatcca ctgctgcggc tggctggtga gcagtggggc gcattgcagt
     1021 cagcgctgct gcaggccgat gcccgcgcca gactggcccg gcagagcctg caacgcgagc
     1081 aggcgctgtt cgctgaaggc atcatcccgc agcggcggtt gcaggaggcg cagacggcac
     1141 tggccgagag cgaggcagtg ctgggccagg cgcggatggc gttgcaggca accggcctgc
     1201 caatggcgct ggtcgagcag ctgctcagcg ccgcaaaacc acagcagaac ctgacgctga
     1261 ccgcacccac ggcgggcagg gtgagccaga tcgaggtgca gccgggacag caggtcgaag
     1321 cggccgatgc gctgctgcaa ctgacacagc ccgacccgtt gtggctggcg atccggatgc
     1381 cggtgagcga aggcgaccgc tggcagccgg gcactgcgct gacagtggcc ggcaaggcgg
     1441 tgaccgcccg tctgttgcgg gctggaacca tcctggccgc cgacagccag accttcgtct
     1501 ggcacgccac ggtcgagcgc ggtggtgagc aactgcggcc gggcgagttc gtcgctgtcg
     1561 aactggcggt cgccgagggc gagcagggtt gggaggtgcc actggcggcc atcgcccgcg
     1621 acggcgatca ggcctgcctg ttcgtgcgca ccgccgaggg tttcgaggcc cggccggtgc
     1681 ggattctggc cagcggcggg cagcaggtgc gggtgcaggg cctgctgcag agcggcgaac
     1741 agatcgccgt cagcggtgtg gtcgcgctca agggtgcctg gctcgatgcc cggggaggtg
     1801 agtgatgctg gcgccactga tccgtttttc gctgtcacag cggctgttca tcctgctggc
     1861 gacactgctg gtcgccgccc tcggctggat ggcctttcgc gggttgccga tcgatgcctt
     1921 tcccgatgtc tccagcaccc aggtgaaggt gatcatgaag gcgcccggca tgacgccgga
     1981 ggaggtcgag agccgcatcg ccgtgccgat cgaggtggag atgctcggca tcccccacag
     2041 caagatcctg cgttcggtca ccaagtatgg gctggtcgat gtgaccatcg acttcgagga
     2101 tggcaccgac atctactggg cgcggcaaca ggtgaacgag cggctggcgg ggttgggtga
     2161 gagtctgccc ccgggaatca ccggcggcat ggcaccggtg accacgccgc tcggtgagat
     2221 gttcatgttc accgtcgagg gcgaggggct gtcgctgacc gagcggcgca gtctgctcga
     2281 ctgggtgatc cgcccggcgc tgcgcacggt accgggggtg gccgaggtga atgcactggg
     2341 tggggcggtc cgtgccttcg aggtggtgcc cgatccgctc aagctggctg ccagcggggt
     2401 gacgctcggt cagctgaagg agacgatcca ggccaacaac cgcaacgacg gtgccggtcg
     2461 gctgggtgag ggcgaagagg tgctgctggt gcgcagcgag ggcagcgtca ccacgctgga
     2521 ggatctgcgc agcatcgtgg tggcccgcaa ggccggggtg ccggtgcctc tcggcgcact
     2581 ggccgaggtg agaatcggca ccatgacccg ttatggcgcg gtgacccgca gcggtcaggg
     2641 tgaggcggtt caggggctgg tgctggggtt gcgtggcgcc aacgcgcagc aggtggtcga
     2701 aggggtgcgg cagaagatcg ccgagttgca gcccagcctg ccgccgggcg tgcggatcga
     2761 caccttctac gaccgcgccg cactggtgac ccaggcggtg ggcacggtct cctcggccct
     2821 gatcgaggcg acgctgctgg tggtcgtgct gctggtgctg ttcctcggtg acctgcgcgc
     2881 cgcgctgaca gtggcgctgg tgctgccgct ggcggcgctg atcaccttca tcctgatgcg
     2941 caatttcggc atgtcggcca acctgatgag cctgggcggt ctggcgatcg ccattggcat
     3001 gctggtcgac gcggcggtgg tggtggtcga gaacatcgtg cagcggctgg ccaccgaccc
     3061 cagtggcggc aggttgccgc ggctgcacat catctaccgg gcagtgcgcg aggtggtggt
     3121 gccggtcacc tccggcatcc tgatcatcgt cacggtgttc ctgccactgc tgacattgca
     3181 ggggctcgaa ggaaaactgt tcacaccggt ggcgctcacc atcgtcttcg cgctgctcgc
     3241 ctcgctgctg ctgtcactga cggtgattcc ggtgctcgcc tcctacctgc tcggtgaagc
     3301 gcggcatgaa gagccctggc tgccgcgcaa gctgttgaca ctctacgagc cggctctggc
     3361 ctggggcatg cgacgacaac cgctggtggc ggccgtggcg ctgctgatgc tgctgctggc
     3421 ggcgctggtc tatacccaga tcggcaagag cttcatgccg acgctggatg agggcgacct
     3481 gatcgtcggc atcgagaagc tgccgtcgat cagcctggag cagagcgtcg cgctcgacct
     3541 gaagatccag caggcgatca tgcaggccat tcccgaggtg accggcatcg tcgcgcgtgc
     3601 cggcgccgac gagatcggtc tcgacccgat ggggctgaac cagaccgaca cctatttgct
     3661 gctgaagccg cgagatgagt ggaatctgcc ggacaaggcg gcattgatgg aggcggtgcg
     3721 caaggtgctc gacccactgc cgggcatcga gtacagcttc acccagccga tcgagatgcg
     3781 ggtgtcggag atggtgatcg gcgtgcgcgg cgacctggcg gtgaagatct tcgggcctga
     3841 tctggccaga ctgaatgacc atgccggaca gatcgaaacg ctgctgaagc gcatccccgg
     3901 caaccaggat gtctacacgg tacagaatga tggcgtgcag tacctgcggg tggtgatcga
     3961 ccgcctgcag gccgggcggc ttgggctcac ggtcgaggag atccaggatg cattgcgcat
     4021 ccagatcgaa ggagcacgtg ccggcgtcgt gatcgagggc aaccagcgca caccgatcgt
     4081 gctgcgtggc gccgaggcgg tgcggctgtc gccggccgaa ttcgccgcgc tgcgcatcac
     4141 caccgccgag ggcgcgaccg tgccgcttgc cagtgtcgcc cggctggagc gtgctgcagg
     4201 gccggtcaag atcgaccgtg agatgggcag ccgttacagc gtggtgatcg ccaatgtcag
     4261 cggccgcgat ctggtcagct tcgtcgaaga agccaaggca ttgattgcgc agaaggtggc
     4321 gctgccgagt ggctaccgca tcacctgggg cggacagttc gagaaccagc agcgggcggc
     4381 cgcccggctg acggtggtgg tgccagtggc gctggcactg atcttcgtgc tgctgttctc
     4441 caccttcggc tcggtgcggc aggcgttgct gattctcagc aacatcccgt tcgcactggt
     4501 gggtggcatc gtcgcgctct ggctgaccgg cgagtatctg tcggtgccgg cctcggtcgg
     4561 cttcatcgcg ctgctgggca tcgccgtgct caacggggtg gtgctggtca gccatttcaa
     4621 ccagctgcat ggtgagggac tgccactggc cgaggtggtg gtgcagggcg ccagacgccg
     4681 tctgcggccg gtgctgatga cggcgctgat caccgccttc ggactgatcc cgctgctgtt
     4741 ctcgaccggg cctggttcgg agatccagcg gccactggcg atcgtggtga tcggcggcct
     4801 gatcacggcc accgcgctga cgctgatgct gctgccgatc ctctatctgc gcttcgcact
     4861 gccgaaaccg gaggtgtccg atgtctgaac tctgtctgac cctgctctgt cctccgcaga
     4921 ttgccgaagc actgctcgat ctgctgctga cctggccgca gaacctgctg ttcaccagca
     4981 cgccgatggc cgcccatgga ctcaccgtcg atgtgctgga tcaatccgaa caggtgatgg
     5041 ggcgggcacg ggccatcgcc atcaccgtgc tgctgagcga tgccgacaag cgcacgctgc
     5101 tcggcaccct gcatcaacag tttgccggca gcggattgcg ctactggctg accgccgtca
     5161 ccgaaaccgg agagatcgca tgaaggcgcc actgctgggc atggtgctga ccctgatcgc
     5221 accgatgatt gccgccgaac tcgcaccacc gccaatgacc gggctgctgc ccaccgaact
     5281 ggcgcggccg ctgctcgacc aggatcctgg cgtggcggca gcacaggccg atctgcaggt
     5341 cgcgctgcac gaggctggca ttctcgaacg ctcgccccat gagtggagcc tgcgcaccac
     5401 cggtcagcag cgcaacctcg accgcggtcc gacccatgac gaatggcatc tggcgctgga
     5461 gcggacactg cgcctgccgg gcaaggccgc ggctgaccgc ggtctgggtg ccgccactgt
     5521 cgaggctgcc caggcccgcc gtggcgagct gctgcacgaa accgcccgtg aactgatgac
     5581 gctgtggctg gagtggctgg cggccgagca gacccgtgaa ctggccgctg acgccctgct
     5641 cgcagtcgag cgcgcgcagg cagcggtgca caaacgggtc accgccggcg acgccgccca
     5701 actcgacctg agcctggccc aggcggagct ggcgacgcag caggtgttca gcaacgatgc
     5761 ccagacccaa gccgccgcga gctgggcacg gctctccacc cgctttccgg ccactccccg
     5821 acagccgctg ccaatggccg agccgatacc ggtcgaaggg acggtcgcgc tctggcaacg
     5881 ccgcattctc gaacagagcg acgccctgaa actgaccgag gccgcgctgc agggtgacga
     5941 ggcccgcgca gcccgggccc ggagcgagcg gctgcccgac ccgaccctcg gcgccttcac
     6001 cgcctccgag gcgagtggcc atgaacgcat cgccggcgtc agcctcagcc tgccgattcc
     6061 gggtggatca cgcgcgctgc gcgatgccca ggcgtacgcc atggtcgagg tgtcgcggca
     6121 acgggtcgag ctgaagcggc gtcaactcga aagcgagatc gccagcgccg tcatcaccgc
     6181 ccagggcgcc tatcgcagtt ggcagaccgc ccgtgagggt gcagcggcaa tgcagcagaa
     6241 tgccgcgctg gtggagcgtg cctacacgct gggtgaaacc gatctgcaga ccctgctgct
     6301 ggcgcgccgt caggcgattt cggcacgaca aggcgcgctg caggcgcgga cggcggcact
     6361 caacagccac tatggactgc tgatcgacgc ccatctggtc tgggaactgg cgcacgaaga
     6421 gtgagcatcg ccggccgctg cttgccctgc tagcgcaaaa agtcgagaat ggcaagcacc
     6481 gcgatggggc gagcagtcga agcgatccat cgcagcgtga ttttcgcacc gaagtacgtt
     6541 tcgttaccga
//
LOCUS       DAORCQ010000117         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_8809_length_6355_cov_5.410794, whole genome shotgun sequence.
ACCESSION   DAORCQ010000117 DAORCQ010000000
VERSION     DAORCQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8809_length_6355_cov_5.410794"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="aceE"
                     /locus_tag="PKI23_10240"
     CDS             complement(<1..546)
                     /gene="aceE"
                     /locus_tag="PKI23_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622108.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNN37355.1"
                     /translation="MHDDIDPLETGEWLQALDSVMQQEGRERAAFLIEQLIERAGRRA
                     VKLPSAVTTPYRNTIAVQDEARIPGNIFMDRRIRSLIRWNAFAMVMRANQTDHDLGGH
                     ISSFASSATLYEIGFGYFFRGTNAQQLGDLIYFQGHSAPGIYARSFLEGRLDETRLDN
                     FRQEVAGHGLSSYPHPWLMPDY"
     gene            complement(620..1567)
                     /locus_tag="PKI23_10245"
     CDS             complement(620..1567)
                     /locus_tag="PKI23_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNN37356.1"
                     /translation="MRLGIDLGGTKIEGVALDETGEICCRKRLPTPAGSYEETLEAIV
                     ELVCQIERQTGRSGTLGIGTPGAVSRMSGQIKNSNSTCLNGQFLREDLTRRLGREVRI
                     ANDADCFALSEASDGAGTGHAVVFGVILGTGAGGGIVVNGQLVNGPNAIAGEWGHNPL
                     YFSARDQNSADWFSADRPERAQLLPDGNRRCNCGRLNCNETYISGSGLQLSYQEQGGE
                     SLTAVEIAELARNGDPLAERTLQLYQRQLARALASVINILDPDAIVLGGGVSNIDSLY
                     DEVPRLWQPHVFSDRVDTPLLKAKHGDSSGVRGAAWLWP"
     gene            complement(1600..2505)
                     /gene="ampE"
                     /locus_tag="PKI23_10250"
     CDS             complement(1600..2505)
                     /gene="ampE"
                     /locus_tag="PKI23_10250"
                     /inference="COORDINATES: protein motif:HMM:NF028423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory signaling modulator protein AmpE"
                     /protein_id="HNN37357.1"
                     /translation="MKFLALLITLAIAMLFPFRPLLHRDAFFIAFRDWMARHAPSEPG
                     STLSILLGALLPALLIALLLALLQGWLLGLVGLVAGCAILLYSLERRDLLADLSRYRL
                     AWSRGDAHAAWRIAIEARLLPTECTPTTAIELNRLVAQGIAVRALSGLFTLLFWFFLL
                     GPAAVLFLRLIQLPTAESGVERLEPGSASGRLLELAQWLPMRLLGGCFMLAGDWGRAW
                     PCWSGSLTDFDSSAADLIGRLALAAAAIDQPLDLSDAVSLDSEPSASAAVVKTGNAQI
                     EAMTALLRRGLIIWVTLFALLTLLG"
     gene            complement(2502..3113)
                     /gene="ampD"
                     /locus_tag="PKI23_10255"
     CDS             complement(2502..3113)
                     /gene="ampD"
                     /locus_tag="PKI23_10255"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF008758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNN37358.1"
                     /translation="MQQRVNQHWWHGAKRLPSPNQNERPAAAAITLIVIHNISLPAGE
                     FGGSAIDALFLNQLDISAHPSFSSLAGLRVSSHLLIRRDGAVHQYVSFDRRAWHAGRS
                     CYRGVVDCNDFSIGIELEGTDHTPYESIQYERLVEIVSDLMACYPTLSREAIVGHQHI
                     APQRKSDPGPAFDWAGFRQRLHERLGGMTDGGWTGQTGTGVLQ"
     gene            complement(3195..5486)
                     /locus_tag="PKI23_10260"
     CDS             complement(3195..5486)
                     /locus_tag="PKI23_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HNN37359.1"
                     /translation="MSRDSSNVYQLPRSRPRATVVTPPRMPPLLANLRDLFAEGLRAQ
                     LKCCFDLTDDALFEMANHAGDAEQQNVYLEAMRELRLKRSSVEALFHSRLAERLNGWL
                     LGVRSGALAEEKIHAQIDAETLTLLSEDELEERVAIESMSVRARDRLLQPLDHLSLRL
                     NHLLPGVVGQGHDENPFAPHALCEAFVAATRVLEAGMKAKLVLFKLFERNMLEQLERL
                     YQQINQFLAEAGILPDLKGRGRTAASPQRRPASEMVSTTRREDSSLRDETEDLLASLH
                     DLLARQRRAGSSDEAHADYKNSISIGELANILKTVQSSDGQQHRGFDSRFSAGGSPVV
                     SGAGVTAIWPLIQRLLGQTNSGGQQAIAPLEQDVIHLISMLFEFILNDYNIPEAIKAQ
                     LGRLQIPLLRAALLDRGFLNRANHPARRLLNELASASVGLADNAGSRDQLYLKINEVI
                     ERVTSEFDTDVTLFQKLLDEFTLFMREERRRTELAEHRLRTAEDGRAKMESARAAAQR
                     VIDGLHQGNRIPQKISALLRDGWSHHLAIIHLKEGEASNAWGQSIETVEELVWSIQPQ
                     RQAERMQLLQRLPGLLQRLRDGLNLVAFDPCLTNEFFVELETLHLQICQSKQSDLPMA
                     GEAVAMIEPVTPSSLPDSPKVDAVEEPVPAMQDGAESLLLPSSAETQALLDNLATGSW
                     VEFIRGQERLRCKLVAFIRSADKYIFVNRAGAKVAQKQRGEMLAALAAGELLILDDAQ
                     LFDRALTAVIGTLRNASVQELHS"
     gene            5753..>6355
                     /gene="nadC"
                     /locus_tag="PKI23_10265"
     CDS             5753..>6355
                     /gene="nadC"
                     /locus_tag="PKI23_10265"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNN37360.1"
                     /translation="MPYRSLPPMTEITANVTAALNEDIGSGDLTAQLIPATRQAEATL
                     LCRDAAVVCGRPWVDRCFELLDPTIRIEWLVEEGAEVAPDTLLARLHGAARALLTGER
                     TALNFLQTLSATATRSRQFADLVRDYPVQLLDTRKTLPGLRLAQKYAVRCGGCHNHRL
                     GLYDAFLIKENHIASLGSITAVITQARGIAANRKVEIEVES"
BASE COUNT         1282 a         2045 c         1879 g         1149 t
ORIGIN      
        1 gtagtcgggc atcagccatg gatgcggata ggaggagagt ccatgccccg caacctcctg
       61 gcggaagttg tcgagacggg tctcatccag ccgcccctcc agaaacgagc gggcatagat
      121 gccgggggcg gagtggccct gaaaatagat gaggtcaccc aactgctgtg cgttggtgcc
      181 tcgaaagaag taaccgaaac cgatctcata gagggtggcg ctcgatgcaa agctgctgat
      241 gtggccgccc aggtcatggt cggtctggtt ggcgcgcatc accatcgcaa aggcattcca
      301 gcgaatcagc gagcgaatcc gtcgatccat gaagatgttg ccgggaatgc gtgcctcatc
      361 ctgcaccgca atggtgttgc gatagggcgt ggtgaccgcc gaggggagtt tgaccgcgcg
      421 gcgtccggcg cgctcgatca gctgctcgat caggaaggcc gctcgctcgc gtccctcctg
      481 ctgcatcacc gaatcgagcg cctgcaacca ctccccggtt tcgagcgggt cgatgtcatc
      541 atgcatggag tcactcatcg ccagaccctc cttcgggtca tcgatatttt cacggatagc
      601 gcagcagttc agtcgatcct cagggccaga gccaggcggc accgcgcacg ccgctcgaat
      661 cgccatgttt ggccttcagc agtggcgtgt ccacccgatc cgaaaacaca tggggctgcc
      721 acagccgcgg cacttcgtca tagagcgaat cgatgttgga gaccccgccg cccagcacaa
      781 tggcatccgg atcgagaatg ttgatcacgc tggccaatgc ccgcgccagc tgacgttgat
      841 aaagctgcaa ggtgcgttcc gccagcggat caccgttgcg cgccagctcg gcaatttcga
      901 ctgcggtgag cgattccccg ccctgctcct ggtaactcaa ctgcaagccg ctgccagaga
      961 tgtaggtctc gttgcagttg agccggccgc agttgcagcg gcggttgcca tcgggcagca
     1021 gttgtgcgcg ctctgggcgg tccgcgctga accagtcggc ggagttctga tcccgtgcgc
     1081 tgaagtagag cggattgtga ccccactcac cggcaatggc attcgggcca ttcaccagct
     1141 ggccgttgac gacgatgcca cctccggcgc cggtgccgag gatgacgccg aagaccaccg
     1201 catgtccggt gccggcaccg tcactggcct cggagagtgc aaaacagtcg gcatcgttgg
     1261 cgatgcgcac ctcgcgaccg agtctgcggg tcagatcctc gcgcaggaac tgtccgttca
     1321 gacaggtgga gttgctgttc ttgatctgtc cgctcatccg cgacaccgcc cccggtgtgc
     1381 cgatgccgag ggtgccgctg cgaccggtct gacgttcgat ctggcatacc agttcaacga
     1441 tggcctccag cgtctcttcg taactcccgg ccggggtggg cagccgcttc cgacagcaga
     1501 tctcgcccgt ctcatcgagg gccacgccct cgatcttggt gccgcccaga tcaatgccga
     1561 gtcgcatcaa tgtcgccctc ccaatcagat gctgttggct caacccagca gggtcagcaa
     1621 cgcaaagagt gtcacccaga tgatcagccc acgccgaagc agcgcggtca tggcttcgat
     1681 ctgcgcattg cccgttttca ccacggccgc actggcgctc ggttcactgt cgagcgacac
     1741 agcgtcggac agatcgagtg gctgatcgat ggccgccgct gccagcgcca accggccaat
     1801 cagatcggct gcgctgctgt cgaaatcggt caggctgccg ctccagcagg gccaagcgcg
     1861 cccccagtca cccgccagca tgaaacagcc ccccagcaat cgcatgggca accactgtgc
     1921 caattcgagc aggcgcccac tggcactgcc gggctccagt cgctccaccc cactctcggc
     1981 cgttggcagt tgaatcagcc gcagaaagag cacggcggca ggccccagca gaaagaacca
     2041 gaagagcagt gtgaacagcc cgctcagtgc acgcaccgcg ataccctgcg caaccagcct
     2101 gttcagttcg atcgcagtgg tcggcgtgca ttcggtcggc agcagtcgag cttcgatggc
     2161 gattcgccag gccgcatggg catcgccacg gctccaggcc agacgatagc gcgacaaatc
     2221 ggccagcaga tcccgacgct cgaggctgta gagcagaatt gcacaacccg ccaccagccc
     2281 gaccagcccc agcagccagc cttgcagcag cgccagcagc agcgcgatca gcagcgccgg
     2341 cagcagtgcg cccagcagga tcgacagggt cgaacccggt tcggacggcg catggcgggc
     2401 catccagtcg cggaaagcga tgaagaacgc atctcgatgc aacagcggac gaaacggaaa
     2461 gagcatcgcg atcgcgagcg tgatcagcag cgccaagaac ttcattgcag gacacctgtg
     2521 ccggtttgcc cagtccatcc gccatcggtc atgccaccca gacgctcatg cagtcgctga
     2581 cggaatcctg cccagtcaaa ggccggtccc ggatcgctct tgcgctgcgg tgcgatgtgc
     2641 tgatggccga cgatggcttc gcgagagagc gttggatagc aggccatcaa atccgagact
     2701 atttcgacta accgctcata ctggattgat tcataagggg tatgatcagt cccttcaagc
     2761 tcgattccaa tcgaaaaatc attgcagtcg acaacgccgc ggtagcagga gcgcccagcg
     2821 tgccaggccc gccgatcgaa ggagacatac tggtgaaccg caccatcgcg gcggatcagc
     2881 agatgcgaag agacgcgcag gcccgccaag gagctgaatg aggggtgggc cgagatgtcg
     2941 agttggttga gaaagagtgc atcaatggcc gatccaccaa actcgcccgc cggcagggag
     3001 atgttgtgga tgacgatcag cgtgatcgcc gcagcggcgg gccgctcatt ctgattgggg
     3061 gaaggcaggc gcttggcgcc atgccaccag tgctggttca cacgctgctg catcgacttc
     3121 cgtctccact tgcgcagagt ctggaagatc gatgaccaaa agtccaatcc ggtcagccag
     3181 tgaggctcct cacctcaact gtgcagttcc tgcaccgaag cgttgcgcag cgtgccaatc
     3241 actgccgtca gcgcccgatc gaacagttgg gcatcgtcga gaatcagcag ttcgccggcc
     3301 gccagcgccg ccagcatctc gccgcgctgt ttctgcgcca ccttggcgcc agcgcggttg
     3361 acgaagatgt acttgtccgc gctccggatg aaggccacca gcttgcagcg cagccgctcc
     3421 tgaccgcgga tgaactcgac ccaactgccg gtcgccaggt tgtcgagcag tgcctgtgtc
     3481 tcggcactgg atggaagcag caggctctcc gcgccatcct gcatggctgg cactggctct
     3541 tccacagcat ccaccttggg ggaatcgggc aacgaagatg gagtgaccgg ttcgatcatc
     3601 gccacggctt cgccggccat gggcagatcg ctctgcttcg actggcaaat ctgcagatgg
     3661 agcgtctcca gttcgacaaa gaactcattg gtgaggcagg gatcgaaggc aaccaggttg
     3721 agcccgtcac gcaaccgctg cagcagcccg ggaagccgtt gcagcagctg catccgctcg
     3781 gcctgccgct gcggctggat gctccagacc agctcctcaa cggtttcgat actctgcccc
     3841 caggcgttgc tggcctcacc ctccttgaga tgaatgatgg ccaggtgatg gctccagccg
     3901 tcgcgcagca gcgcggaaat cttctgtgga atccggttgc cctgatgcaa tccatcgatg
     3961 acccgctgcg cggctgcacg tgccgactcc atcttcgccc ggccatcctc cgcagtgcgc
     4021 agacggtgtt cggccagctc ggtacgccga cgttcttcac gcatgaacag tgtgaactca
     4081 tcgagcagct tctggaacag cgtgacatcg gtatcaaact cgctggtcac acgctcgatc
     4141 acctcattga tcttgaggta gagctggtcg cggctgccag cgttgtctgc cagccccacc
     4201 gaggccgagg ccaactcatt caacagccgg cgtgccggat ggttggcgcg gttcagaaaa
     4261 ccacgatcga gcagcgctgc gcgcagcagt ggaatctgca accgtcccaa ctgcgccttg
     4321 atggcctccg gaatgttgta gtcgttgaga atgaactcga acagcatcga aatcaggtgg
     4381 atgacatcct gctccagcgg tgcaatcgcc tgctgcccac cactgttcgt ctggcccagc
     4441 agccgttgaa tcagcggcca gatcgccgtt acacccgcac cggagacgac tggactgccc
     4501 cctgccgaaa accggctgtc aaaacccctg tgctgctgtc catcagaact ttgaacagtc
     4561 ttgagaatgt ttgccaactc accgattgaa atgctgtttt tgtaatctgc atgcgcttca
     4621 tcagaacttc cggcccgacg ctggcgtgcc agcagatcat gcagtgacgc cagcaggtct
     4681 tccgtctcat cccgcagtga cgaatcctcg cggcgcgttg tcgaaaccat ttcagatgct
     4741 ggacgccgct gtggcgatgc cgcagtgcgt ccgcgccctt tcagatcggg cagaatgccg
     4801 gcctcggcca gaaactggtt gatctgctga tagaggcgtt caagctgttc gagcatattg
     4861 cgctcgaaca gcttgaacag caccagcttg gccttcatgc ccgcctccag cacccgggtg
     4921 gccgcgacaa aggcttcaca cagcgcatga ggggcaaagg gattctcatc atggccctgt
     4981 ccaaccaccc ctggcagcag gtggttcagg cgcaatgaga gatgatcaag cggttgcagc
     5041 agccggtcgc gggcacgcac cgacatcgat tcgatggcga cccgttcttc gagctcatcc
     5101 tcgctcagca gggtgagggt ctccgcatcg atctgcgcat ggatcttctc ctccgccagt
     5161 gcaccacttc tgacccccag cagccagcca ttcaaccgtt cggccaggcg tgaatgaaac
     5221 agcgcctcga cgctgctgcg cttgagtcgc agttcgcgca ttgcctccag atagacattc
     5281 tgctgctcgg catcaccggc atggttggcc atctcgaaga gcgcatcgtc ggtgagatcg
     5341 aagcagcact tcaactgcgc gcgcaaccct tcggcaaaga gatcgcgcag attggccagc
     5401 aaagggggca ttctgggggg cgtcaccacg gtggcgcgcg gccgtgacct gggcaactga
     5461 tagacattgc tggaatctct ggacatgacc ggcacctcgc attaccgcag ccgctgaggt
     5521 ggaacagcgg actcccgtca acattggacg aactgatgca tggttcgagt ataggcagcc
     5581 atctccagta cggcagcgac aaaatccaat tccgtgtgcg ggatcacatt ctgccgggca
     5641 caagagataa atcagtgcga agccggcgat ggaagcgcca gaagctgggg gcaatgcgca
     5701 tataatccgg cgacccgggc cttttcttcg acccccatac cgagcattca tcatgcccta
     5761 ccgctccctt ccgccaatga ccgaaatcac cgccaacgtc accgcagcgc tcaacgaaga
     5821 catcggcagc ggcgatctga ccgcacaact gattcctgcc acccggcagg ccgaagccac
     5881 cctcctctgc cgcgatgcag ccgtggtgtg cggtcgtccc tgggtcgatc gctgtttcga
     5941 actgctcgac cccacaatcc gcatcgaatg gctggttgaa gagggcgccg aagtggcgcc
     6001 cgacaccttg ctcgcccgtc tgcatggagc ggcacgggca ctgctgaccg gggagcgcac
     6061 tgcactcaac tttctgcaaa cgctctcggc gaccgcaacc cgctctcgac aatttgccga
     6121 tctggtgcgc gactacccgg tgcaactgct cgacactcgc aagaccctgc cgggcctgcg
     6181 actggcgcag aaatatgcgg tgcgctgtgg cggttgccac aaccatcgtc tgggcctgta
     6241 cgacgccttt ctgatcaagg agaaccacat tgccagcctg ggaagcatca ccgctgtcat
     6301 cacccaggcg cgtggcatcg cagccaatcg aaaggtggag atcgaggtgg agagt
//
LOCUS       DAORCQ010000118         6299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_8927_length_6299_cov_5.607463, whole genome shotgun sequence.
ACCESSION   DAORCQ010000118 DAORCQ010000000
VERSION     DAORCQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8927_length_6299_cov_5.607463"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKI23_10270"
     CDS             <1..542
                     /locus_tag="PKI23_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNN37361.1"
                     /translation="CHYCDTSYAFSGGSLHSLDEIIAATAGHGARHVCVTGGEPLAQP
                     RCLDLLVRLCDLGYRVSLETSGAIDLDGVDARVVKVMDLKTPGSGEVERNLWRNLDLL
                     NPHDQIKLVLRDRRDYEWARFMLDQHRLPERVEAVLFSPCQGELDPARLAQWMLDDRL
                     PVRFQLQLHKVLWGDQPGR"
     gene            547..1236
                     /gene="queC"
                     /locus_tag="PKI23_10275"
     CDS             547..1236
                     /gene="queC"
                     /locus_tag="PKI23_10275"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019333476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNN37362.1"
                     /translation="MNMATSSERRAVVLLSGGLDSSTALAATRVEGYHCYSMAVDYGQ
                     RHQAELEAAQRIARQLGAVEHRVMKIDLAGIGGSALTDRSIAVPEQPSPGIPVTYVPA
                     RNTIFLALALAWAEVLDADAIVIGVNAVDYSGYPDCRPEFIAAFAGMANLATRRAVLG
                     QRIELKTPLIDLTKAQIVQWGAALGVDYALTVSCYQADLTGAACGRCDSCRLRAKGFS
                     DAGLPDPTRYA"
     gene            complement(1279..1719)
                     /locus_tag="PKI23_10280"
     CDS             complement(1279..1719)
                     /locus_tag="PKI23_10280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNN37363.1"
                     /translation="MHIHLAETDAEIAGCHPAMQTLRPQIAEADFVSRIRRQQASGYR
                     LAFVEQDGMAVAVAGFRLGENLAWGRFLYVDDLVTLPNHRSQGLGATLLSWLRAFAVQ
                     AGCQQLHLDSGLHRADAHRFYEREGMSKRSFHFAENLAPDGAGR"
     gene            complement(1758..3677)
                     /locus_tag="PKI23_10285"
     CDS             complement(1758..3677)
                     /locus_tag="PKI23_10285"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNN37364.1"
                     /translation="MKFTRLFSRTGLILLLTLLCAATLLIDRLLPAARIDLTQDRLYS
                     LSDGSRKLLQSLDKPVHLYLFFSEKESKENPFIRAYAKRVRELIDEYRLASNGKLLLE
                     VIDPEPFSEAEDKAAELGINAIPLQAGGPQIYLGLAAKGENGTTERIPFFSPDREPLL
                     EYDISRMIYTVSRGKRPTVGLISAIDVLGGIDMLRRQPTPPWMAFAQLEQLFEFKQVG
                     NETSELDPKLYDLLVLIHPQQLPPRLEYAIDQYLMRGGKLILFVDPHAERMNQGMMEL
                     GDAASNLPALMKSWGVEMVDKKVLLDDGHAINVTVGEEARQVRHPAILALDKSGFDSS
                     DVIMSRLEYVLVSSSGVLKPIEGATTRFTPLMQSSSQSMLIDADKMLGLTDPSLLYQG
                     FEPDGQLYTVAARITGPLQSAFPAGPPAEPKPPANAPKAAAEPPAPAAKPLLPHLAAS
                     QSDSNILVVADTDLLTDRLWVRVQNFMGQQVGTPFADNGDLIANAVDNLSGNPDLIAI
                     RGRGQFYRPFTRVIALQQQASAHFREKQEQLQASLKETEQKLGALQAQRNGEGEQQKS
                     ATLTPEQEATIEQFQQEKLRLRKELRTVQHQLNQEIEQLGTWIKALNILLMPLLITLF
                     ALLHRTVKQRRAALR"
     gene            complement(3691..4425)
                     /locus_tag="PKI23_10290"
     CDS             complement(3691..4425)
                     /locus_tag="PKI23_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN37365.1"
                     /translation="MSATTIVMRRELASYFATPLAYIFIVIFLVVASVFAFSVGNLYE
                     MGQADLLNFFNFHPWLYLFLIPALAMRLWAEERKTGSIELLLTLPIRLHEAVLGKFLA
                     AWLFSGLALLLTFPIWITVNYLGDPDNGIILASYLGSWLMAGAFLAIGSCMSACTSNQ
                     VIAFILTIVICFCYVVAGLPLLLNFFEGWASQPLLDAVASLSFMTHFNAISRGVLDFR
                     DLLFFVLMIVGWLTATAIVIDMKKAQ"
     gene            complement(4425..5348)
                     /locus_tag="PKI23_10295"
     CDS             complement(4425..5348)
                     /locus_tag="PKI23_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN37366.1"
                     /translation="MIRVSSLTKSFDGFVAVDRLSFELKSGEVLGFIGPNGAGKSTTM
                     KIITGFLAPTEGSVTIFDQDIWRTPLAAKRLIGYLPEGAPAYGDMTAAEFLRFIAEIR
                     GFSKQQRSEAIDRVVAALDLETVLGKRIETLSKGFRRRVGLAQALIHDPRVLILDEPT
                     DGLDPNQKHQVRELIRGLSKEKIIIISTHILEEVTALCSRVIVIANGRLLTDATPAQL
                     EAHSRYHRAVTVESREPALGEKLRMLPGVQAVEEDQVNQRYTLIPVTGQEILPALNQR
                     IASEGWAISQIYVEKGELNDVFRQLTTGEAA"
     gene            5685..>6299
                     /locus_tag="PKI23_10300"
     CDS             5685..>6299
                     /locus_tag="PKI23_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNN37367.1"
                     /translation="MKLYHFRRSPYSSRCRMLINFKGLDIELIEPPEDFSSIHPVARI
                     PSLHIDALVIPEAEVICEYLEDQFPTPSLRAENPAKRALVRTINRINDIYILAPMFDL
                     APHVEMQSRDQAVVEQKFEELVLGLVRLNHFIEGPDYAVGNTLTLADCALVPTLYVMT
                     RYLPSFDRADQLKKHPKISSYWERIQHNPRAVPIIDDLAAAMRDR"
BASE COUNT         1134 a         1969 c         2051 g         1145 t
ORIGIN      
        1 gctgccacta ctgcgacacc agttatgctt tcagcggcgg cagcctgcac tcactcgacg
       61 agatcatcgc cgccacggcc ggccatggtg cccgccatgt ctgcgtcacc ggtggcgaac
      121 cgctggcgca gccgcgctgt ctcgacctgc tcgtgcggct ctgtgacctg ggttaccggg
      181 tctcgctcga aaccagcggt gccatcgacc tcgatggtgt cgatgcgcgg gtggtcaagg
      241 tgatggatct gaagacgccc ggttcggggg aggtcgagcg caacctctgg cgcaacctcg
      301 atctgctcaa tccgcatgac cagatcaagc tggtgctgcg tgatcgccgc gactatgagt
      361 gggcccgctt catgcttgat cagcatcgac tgcccgaacg ggtcgaagcg gtgctgttct
      421 cgccctgtca gggggaactc gatccggcac ggctggcgca gtggatgctc gatgaccggc
      481 tgccggtccg gttccagttg caactgcaca aggtactctg gggcgatcag cccggacgtt
      541 gaggggatga acatggcaac cagcagcgaa cggcgcgcgg tggtgctgct ctccggtggg
      601 cttgactcca gcacggcgct ggcggccacc cgtgtcgagg gctatcactg ctactcgatg
      661 gcggtcgact atggccagcg tcaccaggcc gaactggagg cagcacagcg gatcgcccgg
      721 caactgggcg cggtcgaaca tcgggtgatg aagatcgatc tggccggcat cggtggctcg
      781 gcgctgaccg accgcagcat cgcggtgccg gaacagccga gcccgggcat tcccgtcacc
      841 tatgtgcccg cgcgcaatac catttttctg gcgctggcac tggcctgggc ggaggtgctc
      901 gatgccgatg ccatcgtcat cggcgtcaat gcggtcgact actccggcta tcccgactgc
      961 cgaccggagt tcatcgccgc ctttgccggc atggccaacc tcgccacccg tcgcgcggtg
     1021 ctgggccagc gcatcgaact gaagacgccg ctgatcgacc tgaccaaggc gcagatcgtg
     1081 cagtggggcg cggcgctggg tgtcgactac gctctgacgg tctcctgcta tcaggccgat
     1141 ctgaccggcg cagcctgtgg ccgctgcgac agttgccggc tgcgcgccaa ggggttcagc
     1201 gacgccggtc tgcccgaccc gacccgctac gcctgaaccg ggcattcagg cgctggaaca
     1261 gcgctccggc cacacagctc atctgcctgc gccgtcgggc gcgagatttt ccgcgaagtg
     1321 aaaactgcgc ttcgacatcc cttcgcgttc gtagaaacgg tgtgcgtcgg ctctgtgcag
     1381 gccggagtcg aggtgcaact gctggcaacc cgcctggacc gcgaatgccc ggagccatga
     1441 cagcagcgtg gcgcccagcc cttgggagcg gtggttgggc agggtcacca ggtcatcgac
     1501 gtagagaaaa cggccccaag cgaggttttc accgaggcga aagcccgcca ccgccacggc
     1561 catgccgtcc tgttcgacaa aggccaggcg gtaccccgag gcctgctggc ggcgaatccg
     1621 cgaaacaaaa tccgcctccg cgatctgcgg gcgcagtgtc tgcatggctg gatggcagcc
     1681 ggcaatctcc gcatcggttt cggcaagatg aatgtgcatc gctggcatcc cgaggctgaa
     1741 gagaagcgcc ggcggagtca gcggagcgcc gcccgccgct gtttcacggt ccgatgcagc
     1801 agcgcgaaca gcgtgatcag cagcggcatc agcaggatgt tgagagcctt gatccaggtg
     1861 cccaactgct cgatctcctg gttgagctga tgctggacgg tgcgcagctc cttgcgcagc
     1921 cgcagcttct cctgctggaa ctgctcgatg gtggcctcct gctccggtgt cagcgtggcg
     1981 ctcttctgct gttcgccttc gccattgcgc tgggcttgca gtgcccccag cttctgctcg
     2041 gtctccttca gactggcctg cagctgctcc tgcttctctc tgaagtgggc agaggcttgc
     2101 tgttgcagcg cgatgacccg ggtgaagggg cggtagaact gaccccggcc gcggatggcg
     2161 atcaggtcgg ggttgccgct gaggttgtcc accgcattgg cgataagatc gccgttgtcg
     2221 gcgaaggggg tgcccacctg ctgtcccatg aagttctgca cccgcaccca gagccggtcg
     2281 gtgagcaggt cggtgtcggc caccaccagg atgttgctgt cgctctgcga ggcggcgaga
     2341 tggggcagca acggcttggc tgccggagcg ggtggctcag cggctgcctt gggcgcattg
     2401 gccggcggct tgggctcggc cggcgggcca gcggggaagg cgctctgcaa cgggccggtg
     2461 atgcgcgccg ccaccgtgta gagctggcca tccggttcga agccctgata gagcagcgat
     2521 ggatcggtca gtccgagcat cttgtcggca tcgatcagca tgctctgcga actcgactgc
     2581 atcagcgggg tgaagcgggt ggtggcccct tcgatcggct tcagcacacc ggaggaggag
     2641 accagcacat actcgagccg gctcatgatg acgtcgctgc tgtcgaagcc actcttgtcg
     2701 agcgccagaa tcgccggatg gcgcacctgg cgggcctctt cacccacggt gacgttgatc
     2761 gcatggccat cgtcgagcag cacctttttg tcgaccatct cgacgcccca gctcttcatc
     2821 agcgccggca ggttggaggc ggcgtcaccc agttccatca tgccctgatt catgcgctcg
     2881 gcatgggggt cgacgaagag gatcagcttg ccaccgcgca tcaggtactg gtcgatggcg
     2941 tactccagcc gcggcggcag ttgttgcgga tggatcagca ccagcaggtc atagagcttg
     3001 gggtcgagtt cgctggtctc gttgcccacc tgcttgaact cgaagagctg ctccagctgc
     3061 gcgaaggcca tccacggcgg cgtgggctgg cggcgcagca tgtcgatgcc gcccagcacg
     3121 tcgatggcgc tgatcagccc caccgtcggc cgcttgccgc ggctgacggt gtagatcatc
     3181 cggctgatgt catattccag caggggttcg cgatcggggc tgaagaaggg gatgcgctcg
     3241 gtggtgccgt tctctccctt ggcggccaat cccagataga tctgcggccc gccggcctgc
     3301 agcggaatgg cgttgatgcc gagttcggcc gccttgtcct cggcctccga gaagggttcc
     3361 ggatcgatca cctccagcag cagtttaccg ttgctggcca gccgatactc atcgatcagc
     3421 tcgcgcaccc gcttggcgta ggcgcggatg aatggattct ccttgctctc cttttcggag
     3481 aagaacagat agaggtgcac cggcttgtcg agcgattgca gcagcttgcg gctgccgtcg
     3541 gagagtgagt agagccggtc ctgggtcagg tcgatgcgtg ctgcgggcag cagccgatcg
     3601 atcagcaggg tggccgcaca cagcagcgtc agcagcagaa tcagcccggt tctggagaag
     3661 agtctggtga atttcatcgt gatgctccgg ttactgtgcc tttttcatgt cgatcacgat
     3721 cgcggtggcg gtgagccagc cgacgatcat cagcacgaaa aacagcaggt cacgaaaatc
     3781 gagcacgccg cggctgatcg cattgaagtg ggtcatgaag ctcagtgagg ccacggcatc
     3841 cagcagcggc tgactggccc agccctcgaa gaagttgagc agcagcggca aaccggccac
     3901 cacgtagcag aagcagatga cgatggtgag aatgaaggcg atcacctggt tgctggtgca
     3961 ggccgacatg caggagccga tcgccagaaa ggccccggcc attagccagc tgcccagata
     4021 gctggcgagg atgatgccgt tgtccgggtc gccgaggtag ttgacggtga tccagatcgg
     4081 aaaggtgagc agcagcgcca gaccactgaa cagccaggcg gcgaggaact tgcccagcac
     4141 cgcctcgtgc agccggatcg gcagcgtcag cagcagctcg atcgagccgg tcttgcgctc
     4201 ctcggcccac agccgcatcg ccagcgccgg aatcagaaag aggtagagcc agggatgaaa
     4261 gttgaagaaa ttgagcagat cggcctgacc catctcgtag aggttgccga cactgaaggc
     4321 gaagacgctg gccaccacca gaaagatcac gatgaagatg taggccagcg gggtggcgaa
     4381 gtaactggcc agctcgcggc gcatgacaat ggtcgtggcg gacatcaggc ggcttctccg
     4441 gtggtcagtt ggcggaacac gtcgttcagt tcacccttct cgacatagat ctggctgatc
     4501 gcccagcctt cgctggcgat tctctggttg agtgccggca gaatctcctg ccccgtgacc
     4561 ggaatcagcg tgtagcgctg gttgacctga tcctcttcca ccgcctgcac gccgggcagc
     4621 atgcgcagct tctcaccgag cgccggctcg cggctctcga cggtcaccgc ccggtggtag
     4681 cgggagtggg cctcgagctg cgccggcgtc gcgtcggtca gcagccggcc gttggcgatg
     4741 acgatcaccc ggctgcacag cgcggtcacc tcttcgagga tgtgggtcga gatgatgatg
     4801 atcttctctt tcgacagccc gcggatcagc tcgcgcacct gatgcttctg attggggtcg
     4861 agcccatcgg tcggctcatc cagaatcagc accctggggt catggatcag cgcctgtgcc
     4921 aatccgaccc ggcgccggaa gcccttcgac agcgtctcga tgcgtttgcc cagcaccgtt
     4981 tccagatcga gcgcggccac cacccgatcg atcgcctcgc tgcgctgctg tttggagaag
     5041 ccgcgaatct cggcgatgaa gcgaagaaac tcggcagcgg tcatgtcgcc ataggccggc
     5101 gccccctccg gcagatagcc gatcaaccgc ttggcggcca gcggcgtgcg ccagatgtcc
     5161 tgatcgaaga tggtcacgct gccctcggtc ggggccagaa agccggtgat gatcttcatc
     5221 gtggtcgact tgccggcacc attggggccg atgaagccga gcacttcgcc gctcttcagc
     5281 tcgaacgaga gccgatcgac ggcgacgaaa ccgtcaaatg atttggtcag ggaagagaca
     5341 cggatcatgg ccgaaacctg tggggcatgt gatgcatgga gagctggcag atggcagcgg
     5401 aatcctggcg aacgaacgag cagaaaaatg cagtggggcg ggactatagg cagccccctg
     5461 cggctttgca agcgggagtg gcgggcggtt tttttgtcgg catggccgtc gcattgcctg
     5521 tccggcacag agggtgcctg accgggagat gaaggcggtt ccgcccttgc attgtgcaaa
     5581 gtgcgcggcg cacaatggcc cgcaatcaat acgggcatgg atgggccctc tcagctcagc
     5641 acccgctcct gctcgcgact cacctcatcg atccgggaaa cgccatgaaa ctctaccatt
     5701 ttcgccgttc gccctactcc tcgcgctgcc gcatgctgat caacttcaag gggctcgaca
     5761 tcgaactgat cgaaccgccg gaggattttt ccagcatcca tccggtggcc agaatcccct
     5821 cgctgcacat cgacgcgctg gtcattcccg aggccgaagt gatctgcgaa tacctcgaag
     5881 accagtttcc caccccctcg ctgcgggccg aaaacccggc caaacgggcg ctggtgcgca
     5941 ccatcaaccg catcaacgac atctacatac tcgcgccgat gttcgacctg gccccccatg
     6001 tcgagatgca gagccgtgac caggccgtgg tggagcagaa gttcgaagag ctggtgctgg
     6061 gactggtgcg gctcaaccac ttcatcgagg ggccggatta cgcggtcggc aataccctga
     6121 cgctggccga ttgcgcgctg gtgccgacgc tctatgtgat gacccgctac ctgccgagct
     6181 tcgatcgcgc cgatcagctc aaaaaacatc cgaaaatcag cagctactgg gagcgcatcc
     6241 agcacaaccc cagggcggtg ccgatcatcg acgacctggc cgccgcgatg cgcgatcgg
//
LOCUS       DAORCQ010000119         6157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_9227_length_6157_cov_5.382334, whole genome shotgun sequence.
ACCESSION   DAORCQ010000119 DAORCQ010000000
VERSION     DAORCQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9227_length_6157_cov_5.382334"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKI23_10305"
     CDS             <1..271
                     /locus_tag="PKI23_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37368.1"
                     /translation="MEDAATAEISRTSIWQWIQNHKTLDNGKVVTKELFRQMLAEERE
                     VVRKEIGEERWQNGKFEQATQLLDQITTSDELVDFLTLPAYQLLG"
     gene            340..1659
                     /gene="aceA"
                     /locus_tag="PKI23_10310"
     CDS             340..1659
                     /gene="aceA"
                     /locus_tag="PKI23_10310"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNN37369.1"
                     /translation="MSNYNRVEEIARIEQDWKTNPRWKNVKRTYSAADVVRLRGSVQV
                     EHTLARRGAEKLWKLVNGAAAEKFGKDYVNCLGALTGGQAVQQVKAGIQAIYLSGWQV
                     ASDNNSALSMYPDQSLYPVNSVPTVVSRINNSFSRADQIQWQKGVNPGDAGYIDYFAP
                     IVADAEAGFGGVLNAYELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAIQ
                     KLIAARLAADVLGVPTIVLARTDANAADLLTSDCDEYDRPFVVGERTAEGFYRTRAGI
                     DQAIARGLAYAPYADLLWCETATPDLAEAKKFAEAVRKAHPEQLLAYNCSPSFNWKKN
                     LDDATIAKFQRELAAMGYKYQFITLAGVHSMWYNMFDLAYRYAREDMSAYVELQETEF
                     AAAKRGYTFVAHQQEVGTGYFDDVTTVIQGGSSSVTALTGSTEEHQF"
     gene            complement(1763..2368)
                     /locus_tag="PKI23_10315"
     CDS             complement(1763..2368)
                     /locus_tag="PKI23_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNN37370.1"
                     /translation="MSNLTPIPRVPMLSIEEAQKRGAEIGLSESLCPLSVFRVLLQHP
                     TLAKNVGNLLMGLLFEGNVLDARLRELIIMRLGWVTGSNYEWTQHWRVALMFGLSEEE
                     VLAVRDWQSATNLTAADRAVLQATDDTLQLGAISSSTWKLIETHLATPQERVELVLAI
                     GNWRLFSQLLLSLQIPLEEGVASWPPDGKIPAAALNPPFLK"
     gene            2536..3633
                     /locus_tag="PKI23_10320"
     CDS             2536..3633
                     /locus_tag="PKI23_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNN37371.1"
                     /translation="MTLPRTSFAMVQTAARTLEGREIPIPAISDDSAILRIEACGICG
                     SDYEQYEGALSVPMNVIPGHEPLGVIAAIGDKAAKRWGVDVGDRVAVETMLACHFCEN
                     CLAGRYHLCNSRRIYSYIPLSDAPGLWGGYSQYMYLDPCSVLHKMDKKLPPEIAVIFN
                     PLGAGFRWAVEMPQTRIGDTVVIMGPGQRGLSSVLACRQAGAGTIIVTGLAADAKKME
                     VARAFGADHTIDVQNENAVERIQQITHGRGADVVVDVTSYATQPVVEALSYCKSGGTI
                     VLAGIKGFKPIPDFISDMIVVKEITVKGAIGVTSSGYRSAIQLIESGKVPLQLMHTHD
                     FALQDAELAIKTLARQVPGDESIHSCLIPEF"
     gene            3664..4506
                     /locus_tag="PKI23_10325"
     CDS             3664..4506
                     /locus_tag="PKI23_10325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNN37372.1"
                     /translation="MSFVKEWLEGAPYNRTLGVEQVSLSDDLLVLRLPFNEANTNPGG
                     ALHGGVAASLSVIGGQGVVRAALGAESGPWHTAGAQINYLAAAINEGVLAKARLLRRG
                     KELCFVDVAIETEAGKPIAQANLAMRGRFGAAAAQLRRASGDDGLVEPGPMGSHIGAI
                     SFIGNRGIKVELMKEGRSRLVMPYREDNGDLHGVHEGAAMALFDTTGAMAAWAETGPG
                     PYKASTPAIQMQMLAPPPKADLVAYGRVVQRDNELFWSEVEVAEQKSGLVCLRGTVFY
                     RIVT"
     gene            4582..>6157
                     /locus_tag="PKI23_10330"
     CDS             4582..>6157
                     /locus_tag="PKI23_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNN37373.1"
                     /translation="MIKNKIYKNLFWIAVAVVGAFAFGGLALHRGESINALWLIVAAL
                     CIYAIGYRFYSAWIATRVLMLDETRLTPAERLKNGRDFMPTQKWVLFGHHFAAIAGPG
                     PLVGPTLAAQFGYLPGTLWILIGSLLCGCVQDMVILFCSTRRDGRSLGQMARDELGAV
                     AGVAALIGTFMILVILIAVLGLVVVNAMKHSPWATATVAATIPIAMLVGLYMHGLRPG
                     KVLEGSLIGVALLLFSVIGGGWIDQLPAVRGYFDWNGPDLAWAVIIYGFAAAVLPVWL
                     LLAPRDYLSTFLKLGTVFMLAIAVVFLAPEIKMPAVTRFIDGSGPIFGGTLFPFVFIT
                     IACGAISGFHSLVASGTTPKMIENEKQIRLVGYGSMMLESFVALMAMIAATMLDPGVY
                     FAINTAAGVVGSAPAAAVATISSWGFPVTVEQMQNLAQQMGESSLFGRTGGAPSLAVG
                     MATIFNSAFGSNLLALWYHFAIMFEAVFILTTLDAGTRVGRFMLQDLLGNLWKPLGRT
                     SWTPSVLLSSALVVAAW"
BASE COUNT         1140 a         1830 c         1970 g         1217 t
ORIGIN      
        1 gatggaggat gcggcgaccg ccgagatctc gcgcacctcg atctggcagt ggattcagaa
       61 tcataagacg ctcgataacg gcaaggtcgt caccaaggag ctgttccggc agatgttggc
      121 cgaagagcgc gaggtggtac gcaaggagat cggcgaagag cgctggcaaa acggcaagtt
      181 cgagcaggcg acccaattgc tcgatcagat caccacgagt gatgaactgg tcgactttct
      241 gaccctgccg gcttaccagc ttctgggctg atgagttgaa acgataacct tccggtcgct
      301 gaccgggccc tgtccaacaa cccgaactag agagtgacga tgtcaaatta caaccgtgtc
      361 gaagagattg cccgtatcga acaggattgg aaaaccaatc cgcgctggaa aaacgtcaag
      421 cgcacctaca gcgcggcgga cgtggtccgt ctgcgtggtt cggtccaggt ggaacacacg
      481 ctggcccgcc gtggtgccga aaagctgtgg aagctggtca acggcgccgc tgccgaaaaa
      541 ttcggtaagg actatgtcaa ctgcctcggc gcgctgactg gtggccaggc cgtccagcag
      601 gtgaaggcgg gaattcaggc gatctacctc tccggctggc aggtcgcctc cgacaacaac
      661 agcgccctct cgatgtatcc agaccagtcg ctctacccgg tcaactcggt gccgacggtg
      721 gtctcacgca tcaacaactc cttcagccgt gccgatcaga tccagtggca gaagggtgtc
      781 aacccgggcg atgccggcta catcgactac ttcgcgccga tcgtggccga tgccgaagcc
      841 ggtttcggcg gcgtgctcaa tgcctatgaa ctgatgaagg cgatgatcga agccggtgcg
      901 gccggtgtcc acttcgaaga ccagcttgcc tcggtgaaga agtgtggcca catgggtggc
      961 aaggtgctgg tgccgacgca agaggccatc cagaagctga tcgccgcgcg tctggcagcc
     1021 gacgtgctgg gcgtgccgac gatcgttctg gctcgcaccg atgccaacgc ggccgacctg
     1081 ctgaccagcg actgcgacga atatgaccgg ccgttcgtgg tcggcgagcg caccgccgaa
     1141 ggcttctacc gcacccgcgc cggcatcgac caggcgattg cccgtggtct ggcctatgct
     1201 ccttatgccg acctgctctg gtgcgagacc gccacccccg atctggccga ggcgaagaaa
     1261 tttgccgaag ccgtgcgcaa ggcgcatccg gaacagctgc tggcctacaa ctgctcgccg
     1321 tcattcaact ggaagaagaa tctggacgat gccaccatcg ccaagttcca gcgtgaactg
     1381 gcggccatgg gctacaagta ccagttcatc actttggccg gtgtgcacag catgtggtac
     1441 aacatgttcg acctggccta ccgctatgcc cgcgaagaca tgtcggccta tgtcgagctg
     1501 caggagaccg agttcgccgc tgccaagcgt ggctacacct tcgtggcaca tcagcaggag
     1561 gtgggaaccg gctacttcga cgacgtcacc accgtgatcc agggcggcag ctcatcggtg
     1621 accgcgctga ccggttcgac cgaagagcat cagttctgat cattcgctgc ggttgacagg
     1681 gtggaggttc cgctctgtca acgcaccaac agaaaccccc gcttcgtagc gggggtttct
     1741 gttttcagtc cagcagaagc ggttacttca ggaagggtgg attcagtgcg gcggcaggga
     1801 ttttgccatc gggtggccag gaggcgaccc cctcttcgag cgggatttgc aggctgagca
     1861 gcagttgcga gaagagccgc cagttgccga tcgccagcac cagttcgacc cgctcctgtg
     1921 gcgtggccag gtgggtttcg atcagcttcc aggtcgagct ggagatggcg cccagttgca
     1981 gggtgtcgtc ggtcgcctgc agcacggcgc gatcggctgc cgtcaggttg gtggccgact
     2041 gccagtcacg cacggccagc acctcctctt cgctcaggcc gaacatcagc gcgacccgcc
     2101 agtgctgggt ccactcgtag ttggagccgg tgacccagcc gaggcgcatg atgatcagct
     2161 cgcgcaggcg ggcatcgagt acattgcctt cgaacagcaa ccccatcagc aggttgccga
     2221 cattcttcgc cagcgtggga tgctgcagca gcacgcgaaa gacgctgagg ggacacagcg
     2281 actcgctgag accgatttcg gccccccgtt tttgtgcttc ctcgatcgac agcatcggca
     2341 cgcgggggat gggggtcagg ttcgacatgg caggctctcc ttggttgtta ttatggtcct
     2401 tgaagcaagg cacttggctt tttatcctaa ccggcaagcc aaccttggac aacttgtttt
     2461 ggtcatgtcc tttatggttt gattcggcgg cttttcattg ccaggaaccg ataacaacaa
     2521 tccaaggagt gagtcatgac cttacccaga accagtttcg ccatggtgca gactgcggca
     2581 cgcacactgg aagggcggga gattccgatt ccggccatca gcgacgattc agccattctg
     2641 cgcatcgagg cctgcggcat ctgcggcagc gactatgagc agtatgaagg tgcgctcagc
     2701 gtgccgatga atgtgattcc cggtcacgag ccactcggtg tgattgctgc gatcggcgac
     2761 aaggcggcca aacgctgggg tgtcgatgtc ggcgaccggg tcgccgtcga aaccatgctg
     2821 gcctgccatt tctgcgagaa ctgtctggcc ggccgttacc acctctgcaa cagccgtcgc
     2881 atctactcct acattccgct cagcgacgca ccgggtctgt ggggtggtta ttcgcaatac
     2941 atgtacctcg acccctgttc ggtactgcac aagatggata aaaaattgcc gccggagatc
     3001 gcggtgattt tcaatccgct cggggccggc tttcgctggg cggtggagat gccgcagacc
     3061 cgcatcggtg acacggtggt gatcatgggg ccggggcagc gcgggctttc gagcgtgctg
     3121 gcctgccgcc aggccggggc gggcaccatc atcgtcaccg gtctggcagc cgatgcgaag
     3181 aaaatggagg tggcccgcgc ctttggtgcc gaccacacca tcgatgtgca gaacgagaat
     3241 gccgtcgaac gcatccagca gatcacccat ggccgtggcg ccgacgtggt ggtggatgtc
     3301 acctcctacg ccacccagcc ggtggtggag gcgctgagtt actgcaagag cggcggcacc
     3361 atcgtgctgg ccggcatcaa gggattcaag ccgattcccg acttcatctc cgacatgatc
     3421 gtggtcaagg agatcaccgt caaaggcgcg atcggcgtca ccagcagcgg ctaccgcagc
     3481 gccatccagc tgatcgaatc gggcaaggta ccgttgcaac tgatgcacac ccatgacttt
     3541 gccttgcagg atgcggaact ggcgatcaag actctggccc gtcaggtacc gggcgatgaa
     3601 tcgatccact cctgcctcat tccagaattt tgattgaaaa caattgatga caaggagttt
     3661 cgaatgagtt ttgtaaaaga gtggctggag ggcgcgccct acaacagaac actgggcgtc
     3721 gagcaggtca gcctcagcga cgacctgctg gtattgcgcc tgccattcaa tgaggccaac
     3781 accaatcccg gcggtgcgct gcatggcggc gtggccgcct cgctgtcagt gatcggcggg
     3841 cagggggtgg tgcgtgcggc actgggggcg gagtccgggc cctggcacac ggcaggtgcg
     3901 cagatcaact atctggccgc ggccatcaac gagggagtgc tggccaaggc ccggctgctg
     3961 cgcaggggca aggagctctg ctttgtcgat gtcgccatcg aaaccgaggc cggcaagccg
     4021 attgctcagg ccaatctggc aatgcgcggc cgttttggcg ccgcagctgc gcaactgcgt
     4081 cgcgccagcg gcgatgatgg gctggtggaa ccgggtccga tgggcagcca catcggtgcg
     4141 atctctttca tcggcaaccg cggcatcaag gtcgaattga tgaaagaggg gcgctcgcgg
     4201 ctggtgatgc cctaccgcga agacaatggc gacctgcatg gcgtgcacga aggggcggcg
     4261 atggcactgt tcgacaccac cggcgcgatg gcggcctggg ccgagactgg tcccggtcct
     4321 tacaaggcct ccacgccggc gattcagatg cagatgctgg cgccgccacc caaggccgat
     4381 ctggtggcct atggccgggt ggtgcagcgt gacaatgagc tgttctggag cgaggtcgag
     4441 gtggccgagc agaagagtgg cctggtctgt ctgcgcggta cggtcttcta tcgcatcgta
     4501 acctgagtcg aaggccaccg cattccgtgc gggatgcggt ggccattttt atttcacgga
     4561 tatcaaaaaa tcttcatctt gatgataaaa aacaaaatat ataaaaatct gttctggatc
     4621 gccgttgccg tggtgggcgc cttcgctttc ggtggactgg cgctgcaccg tggtgagtcg
     4681 atcaacgcgc tctggttgat cgtggcggcg ctctgcatct atgccatcgg ctatcgtttc
     4741 tacagtgcct ggattgccac cagggtgctg atgctcgacg agacccggct caccccggcc
     4801 gaacggctga aaaatggccg cgacttcatg ccaacgcaga aatgggtgct gtttggccac
     4861 cattttgccg ccatcgccgg accgggccca ctggtcggcc cgaccttggc ggcccagttc
     4921 ggctacctgc ccggcacgct gtggattctg atcggctcgc tgctgtgtgg ctgcgtgcag
     4981 gacatggtga ttctgttctg ctcgactcgg cgcgatggcc gcagtctcgg gcagatggca
     5041 cgcgacgaat tgggggccgt tgccggcgtg gctgcgttga tcggtacctt catgattctg
     5101 gtgatcctga tcgcggtgct ggggctggtg gtggtcaatg cgatgaagca cagcccctgg
     5161 gccaccgcga cggtggcggc gaccattccg attgcgatgc tggtggggct ctacatgcat
     5221 ggcctgcggc ctggcaaggt gctggaaggg agcctgatcg gcgtcgcgct gctgctgttt
     5281 tcggtgatcg gcggcggctg gatcgaccag ttgccggcag tgcgtggtta tttcgactgg
     5341 aacggacccg atctggcctg ggcggtgatc atctatggct ttgccgccgc ggtgctgccg
     5401 gtctggctgc tgctggcgcc gcgggattac ctctccacct tcctcaagct gggaacggtg
     5461 ttcatgctgg cgattgcggt cgtctttctc gcccccgaga tcaagatgcc ggccgtgacc
     5521 cgcttcatcg atggctcggg cccgatcttt ggcggcacgc tgttcccctt cgtcttcatc
     5581 accatcgcct gcggggccat ctccggtttt cactcactgg tcgcctccgg caccacgccg
     5641 aagatgatcg agaacgagaa gcagatccgc ctggtcggct atggcagcat gatgctggag
     5701 tcgttcgtcg cactgatggc gatgattgcg gcaaccatgc tcgaccccgg cgtctatttc
     5761 gccatcaaca ccgcggccgg ggtggtcggc agtgcaccag ccgccgccgt ggccaccatc
     5821 agcagctggg gctttccggt caccgtcgag cagatgcaga acctggcaca gcagatgggt
     5881 gaaagctcgc tgttcggccg caccggcggt gcgccttcac tggcggtcgg catggccacc
     5941 atcttcaaca gcgcctttgg cagcaatctg ctggcgctgt ggtaccactt cgccatcatg
     6001 ttcgaggcag tgttcattct gaccacgctc gatgccggca cccgggtggg gcgcttcatg
     6061 cttcaggatc tgctgggcaa tctgtggaag ccgctgggcc ggacctcctg gacaccctcg
     6121 gtactgctca gctcggcgct ggtggtggcg gcctggg
//
LOCUS       DAORCQ010000120         6148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_9246_length_6148_cov_4.957328, whole genome shotgun sequence.
ACCESSION   DAORCQ010000120 DAORCQ010000000
VERSION     DAORCQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9246_length_6148_cov_4.957328"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKI23_10335"
     CDS             complement(<1..727)
                     /locus_tag="PKI23_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNN37374.1"
                     /translation="MNKQPFSGLKVWDMSWVGVGPLTARYLADHGATVVRLDSSKRPD
                     ILRTAPPFQKGQPGLDNSMFYGDYNCSKLGLGLDMAQTQGQEIARRLALWADVLIESF
                     TPGNLAKWGLDYETLRHEHPSLVMLSTCMQGQTGPRAHYPGFGNLMAAHSGFYAVTGW
                     PDGTPVPVYGAYTDFIAQRFTTCALVAALDHRRRTGQGQYIDVSQFEAASQFLGTELL
                     DYAVNERVADRAGNAAPSAAPHGV"
     gene            complement(744..2030)
                     /locus_tag="PKI23_10340"
     CDS             complement(744..2030)
                     /locus_tag="PKI23_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNN37375.1"
                     /translation="MSQPTRAAAAQSQAGPLAPYRVLDLTTERGWMSGKILADLGAQV
                     LKVEPVGGDPGRHRGPFYQDRADPEHSLRWWFLNRGKRSITLNLETEDGRALFRRLLS
                     QTDLLLESFAPGHLARLGIDHAQLMAEHPRLVWTSITPFGQLGPHARFQGPDLILTAL
                     CGSIFLTGDPDRAPVRVSVPQADLHGAAEGALNSLFALYHVSGGGSGQHVDVAAQLSA
                     TRALMNATPFPKLENKNLVRQGIWAESGGFKRRAIYGCKDGHVCIMLTGGVLGAQTIG
                     ALLEWAAADMTVPAILLDTDWKALDFGALIRNPEKAELFTLLSDFLLEFFMLHDKATL
                     YAGALQRRVLLAPISTVKDISADEQLIAREFFQQVEHPGIGEVRYPGPWAKLSQTPLP
                     ALSRAPTIGEHNEEVYCGRLGLDRQQLVRLAGAGVL"
     gene            complement(2284..4671)
                     /locus_tag="PKI23_10345"
     CDS             complement(2284..4671)
                     /locus_tag="PKI23_10345"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN37376.1"
                     /translation="MPVHAKRLKVAAVVGLSLLLLACGGAEERKAKYLASGKALMEQA
                     DYAKARLELRNVLKIDPKDVGALYLLGQLEEKQQNWRKAYAYYQQVVELDSSHVDARL
                     KLAQIYLLSNDADKALEQVTEVLKLAPDKSDALVMRGIIAGRKGDNEAAFRDASQVLE
                     KDPANIPAATVLSANYEAKGDLTNAIAVLERSSSQNPKEASLQLLLARLYEKSGQSEK
                     SAQALKQIIALQPQEFSHRLRLARYYNAHGQQAEAEQILRDAIAADATAVPPKLALIE
                     LIGKQQGFEAAEQSLRGFIEQEPKNHTLRFGLADLYRSAQREQQVMEVYREIIKLDGE
                     GKEGIKARNSLAGYLLAQKKVDEADTLTSEVLKADPKNQDGLINRAAVALVREQPDAA
                     ITDLRAVLKEQPNAIRAMRLLARAHLQKKEVELARDTLQKALQSAPQERELYLELAQI
                     SLESGDAKAAIATLEQLNQALPNDQDGLRRLAELQLNDKQWGSARETAEKLKAAFPDQ
                     PLGYYLAGLSHQGEQVFETSIKEFQQALDRSPNAIEPLVAISRSYLALNQVDAALANL
                     EKTLKQNPQHSVALNLKGEILLGKRDHAAAEAAFRRAIEVSPKFPAPYLNMAKLHMAR
                     KAPQQAAAMLKQGHEATGEMMLWLQLANLYESTGQIDQAINEYRAILASSPQIEVAAN
                     NLAALLSDRDNPTLLKEALTLAKQFENSKNPLYRDTLGWVYYRQGDMAQAIAHLEEAQ
                     RLAPNVAVINYHLGLSYQNAGRTEEAKARLRAALEQAKGESWISDARGALAKLGG"
     gene            complement(4816..>6148)
                     /locus_tag="PKI23_10350"
     CDS             complement(4816..>6148)
                     /locus_tag="PKI23_10350"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNN37377.1"
                     /translation="GQFLAVVPLVWYGGLRVEHLPLLDSVTLPAAVAIPFTVVAMVGV
                     INGVNHADGLDGLAAGEVLLSLIVIAFLAYLAGGSITLLIAMAVIGGILGFLRFNSHP
                     AQVFMGDNGSQFLGFTLAFLAVRLTQYTNSALSPAVVALLIGLPVIDILGVFYLRAAS
                     GNNWFKASKNHIHHRLLGLGFTHHETVVVIYSIQALFVFGGWCLAYEPDGLILLLYVA
                     AFAGIYAALTLLERGGWRVGQNGKGGFFPHWLDRGLVRNLFRRGAVAVILLLIPLYLV
                     LVPALLTTVPRDFALVAAVLAVVLLAEMLFVRRRPSILARGVVYIAALFSAYLYQEHS
                     IPLFNGLIWPSWLFYGVLAVASGVAIRYSYEINFRTTPTDFLGLFLVLAVALLQFSGA
                     VESGFMLLAVKFLVILYGCELLAMRARSQRNLLNFSALGGLAILAVRGLLP"
BASE COUNT         1136 a         2030 c         1871 g         1111 t
ORIGIN      
        1 agaccccatg gggcgcggca ctcggcgcgg cgttgccggc gcggtcggcc acccgctcat
       61 tgacggcgta gtcgagcagt tcggtgccga gaaactggct ggcggcctcg aactgggaga
      121 catcgatgta ctgcccctga ccggtacggc gccgatgatc gagcgccgcc accagcgcgc
      181 aggtggtgaa gcgctgggcg atgaagtcgg tgtaggcacc atagaccggc accggcgtgc
      241 cgtcgggcca gccggtcacc gcatagaagc cgctgtgggc ggccatcagg ttgccgaagc
      301 cgggatagtg cgcgcgcgga ccggtctgcc cctgcatgca ggtcgacagc atcaccagac
      361 ttgggtgctc gtgccgcagc gtctcgtagt cgagccccca cttggccagg ttgcccggcg
      421 tgaagctctc gatcagcaca tcggcccaca gcgccagccg ccgggcgatc tcctgtccct
      481 gggtctgggc catgtcgagc cccagcccca gcttggagca gttgtaatcg ccatagaaca
      541 tcgaattgtc gagaccgggc tgccctttct gaaagggcgg cgcggtgcgc aggatgtccg
      601 gtcgcttgct gctgtcgagc cgcaccacgg tcgcaccatg gtcggccaga taacgggccg
      661 tcagcggacc gacgccgacc caggacatgt cccacacctt gagtccggaa aagggctgct
      721 tgttcattgt tcgatgctcc ttgctagagt acgccggcgc cggccaggcg aaccagttgc
      781 tgtcgatcga ggccaagtct gccgcaatag acctcttcgt tgtgttcgcc gatggtgggc
      841 gcacgcgaca gcgccggcag cggcgtctgc gacagcttgg cccagggccc cggatagcgg
      901 acctcgccaa tgccagggtg ctcgacctgc tgaaagaatt cacgcgcgat cagctgttcg
      961 tcggcactga tgtccttgac cgtgctgatc ggcgccagca gcacccgccg ctgcagcgcg
     1021 ccggcataga gggtcgcctt gtcgtgcagc atgaagaatt cgagcaggaa atccgaaagc
     1081 agcgtgaaca gctcggcttt ttcgggattg cggatcagcg cgccgaaatc gagcgccttc
     1141 cagtcggtgt cgagcagtat cgccggcacg gtcatgtcgg ccgccgccca ttcgagcagc
     1201 gcgccgatgg tctgcgcacc cagcacgccg ccggtcagca tgatgcagac atggccatcc
     1261 ttgcagccat agatggcgcg ccgcttgaat ccaccacttt cggcccagat gccctgccgc
     1321 accagattct tgttttcgag ttttggaaag ggggtcgcat tcatcaatgc ccgcgtcgcc
     1381 gaaagctgcg ccgccacgtc gacatgctgg ccgctgccgc caccggagac atggtagagc
     1441 gcgaacaggc tgttgagcgc cccctcggcc gcaccatgca gatcggcctg aggcaccgag
     1501 acccgcaccg gtgcgcggtc cggatcacca gtcagaaaga tcgaaccaca gagcgcggtc
     1561 agaatcagat ccggcccctg gaaccgggca tgcggaccca gttggccaaa aggggtgatc
     1621 gaggtccaga ccagcctggg atgctctgcc atcaactgcg catgatcgat accgaggcgg
     1681 gcgagatggc ccggcgcgaa ggattcgagc agcaggtcgg tctgcgacag cagccgccgg
     1741 aacagcgccc ggccatcttc ggtttcgaga ttgagggtga tcgagcgctt gccgcggttg
     1801 agaaaccacc agcgcagcga gtgttcggga tcggcccggt cctggtagaa aggtccacgg
     1861 tgacggcccg gatcgccgcc aaccggctcg accttgagca cctgcgcacc cagatcggcc
     1921 agaatcttgc cggacatcca gccccgctcg gtggtcagat cgagcacacg atagggcgca
     1981 agcgggccgg cctgcgattg agccgcagcg gctcgggtgg gttgactcat tgccccgcac
     2041 tccatgtggt gctgaatcgg ggttacgaca gcagttgatt cagcgttgat tattgttatt
     2101 ggtggtcatt gctggtcgcc agatcaccct gcgcctgccg tcttgatccg ggtgacatgc
     2161 gcgtccatca tcgcccgaaa tcgatcttcg ttgcaacgtt ccgcgctgct tcatcacgca
     2221 atgggcggtg cccgcccgat gctgcaacag cgccggagca gacgctgttg catcgcgaca
     2281 tcctcaacct cccagcttcg ccagcgcgcc cctggcatca ctgatccagg attcgccctt
     2341 ggcctgttcc agcgcggcgc gcaaccgcgc cttggcctct tccgtgcgcc cggcattctg
     2401 atagctcagc cccaggtggt agttgatcac cgccacgttg ggggccagcc gctgcgcctc
     2461 ctcgaggtgg gcgatcgcct gcgccatgtc gccctgccgg tagtagaccc agcccagcgt
     2521 gtcgcggtag agcggattct tcgagttctc gaactgcttg gccagcgtca gcgcctcctt
     2581 gagcagggtc gggttgtcgc ggtcgctcag cagtgccgcc aggttgttgg ccgccacctc
     2641 gatctgcggt gagctggcca gaatggcacg gtactcgttg atcgcctgat cgatctgccc
     2701 ggtggactcg tagaggttgg ccaactgcaa ccagagcatc atctctccgg tggcctcatg
     2761 gccctgcttc agcatcgcgg cggcctgctg cggcgccttg cgggccatgt gcagcttggc
     2821 catgttgaga tagggtgccg ggaacttcgg cgagacctcg atggcacggc ggaacgcagc
     2881 ctcggcagcc gcatgatcac gtttgcccag cagaatctcg cccttgagat tgagcgccac
     2941 gctgtgttgc ggattctgtt tcagcgtctt ttcgaggttt gccagcgcgg catccacctg
     3001 attgagcgcc agataggagc ggctgatcgc caccagcggt tcgatcgcgt tgggtgagcg
     3061 atcgagcgcc tgctggaact ccttgatgct ggtctcgaag acctgctcgc cctgatggct
     3121 gaggccggcc agatagtagc ccaatggctg gtcggggaag gccgccttca gtttctcggc
     3181 cgtctcccgc gcgctgcccc actgcttgtc gttcagttgc agttcggcca gcctgcgcaa
     3241 tccatcctgg tcgttcggca gtgcctggtt caactgctcc agcgtggcaa tggcggcctt
     3301 ggcatcgccg ctctccaggg agatctgcgc cagttcgagg tagagctcgc gctcctgtgg
     3361 cgcactctgc aatgccttct gcagggtgtc acgcgccagc tcgacctctt tcttctgcag
     3421 gtgcgcccgc gccagcaacc gcatcgcacg gatggcattg ggctgctcct tcagcaccgc
     3481 acgcaggtcg gtgatcgccg catcgggctg ctcacgcacc agcgccaccg cggcgcggtt
     3541 gatcaggcca tcctggttct tgggatcggc cttcagcact tcgctggtca gggtgtcggc
     3601 ctcgtcgacc tttttctgtg cgagcagata gccagccaga ctgttgcgcg ccttgatgcc
     3661 ctccttgccc tcgccatcga gcttgatgat ctcgcggtag acctccatca cctgctgctc
     3721 gcgctgggca cttcgataga gatcggccag tccgaagcgc agcgtgtggt tcttcggctc
     3781 ctgttcgatg aaaccgcgca ggctctgctc cgccgcctcg aagccctgct gcttgccaat
     3841 cagctcgatc agcgccagtt tgggcggaac cgccgtggca tcggcggcaa tggcatcacg
     3901 cagaatctgc tcggcctcgg cctgctgccc atgggcgttg tagtaacggg ccagacgcag
     3961 ccggtgcgaa aactcctgcg gctgcagggc aatgatctgc ttcagcgcct gcgcgctctt
     4021 ttcgctctgg ccgctctttt catagagccg tgccagcagc agttgcagac tggcctcctt
     4081 cgggttctgg ctgctgctgc gctccagcac cgcaatggca ttggtcaggt cacccttcgc
     4141 ctcgtagttg gccgacagca ccgtggcggc cgggatgttg gccggatctt tctccagcac
     4201 ctggctggca tccctgaatg cggcctcgtt gtcacccttg cggccagcga tgatgccgcg
     4261 catcaccagg gcatcggact tgtccggtgc cagcttcagc acctcggtca cctgctccag
     4321 cgccttgtcg gcatcgttgc tcagcaggta gatctgtgcc agcttcagtc gcgcatcgac
     4381 atggctggaa tcgagctcga ccacctgctg atagtaggcg taggccttgc gccagttctg
     4441 ctgcttctct tcaagctgcc ccagcagata gagcgcgccg acatccttgg gatcgatctt
     4501 cagcacattg cgcaactcga gccgcgcctt ggcgtaatcg gcctgctcca tcaacgcctt
     4561 gccactggcc aggtacttcg ccttgcgctc ctcggcccca ccacaggcca gcagcagcag
     4621 gctcaacccg accactgcgg ccactttcaa ccgttttgca tgcactggca cctgctgtcc
     4681 ccttgattga tcagatttga aaaaacgttc cgcgattcga acattgtccg gaaagtctat
     4741 tgcaatttgg ctgacgcacc cagccgcttt cgcaaaactc cggatgccgg cacggcacct
     4801 ccagcgacct gcacgctatg gcagcagacc gcgcacggcc aggatggcca ggccacccag
     4861 tgccgaaaaa ttcaacagat tgcgctgact gcgggcacgc atcgccagca gttcgcatcc
     4921 ataaagaatg accagaaact tgacggcaag cagcatgaaa ccgctctcga ccgccccgga
     4981 aaactgcaac agcgcaaccg ccagcaccag aaacagaccc agaaaatcgg ttggcgtggt
     5041 gcggaagttg atctcatacg agtaacgtat cgccacgccg ctcgccacgg ccagaacgcc
     5101 atagaacagc cagctcggcc agatcagacc attgaaaagc ggaatcgagt gctcctgata
     5161 caggtaggcg gaaaagagcg cggcaatgta gaccacacca cgcgccagaa tcgacgggcg
     5221 gcgccggacg aagagcatct cggccagcag cacgacggcc agcaccgcgg ccaccagcgc
     5281 gaaatcgcgc ggcactgtcg tcagcagggc cggcaccagc acaagataga gcgggatcag
     5341 cagcagaatc actgccaccg caccgcgtct gaacaggttg cggaccaaac ctcgatcgag
     5401 ccagtgggga aagaacccgc ctttcccgtt ctgaccgaca cgccaaccgc cgcgctccag
     5461 cagggtcaag gccgcataga tgccggcaaa ggccgcgaca tagagcagca ggatcagccc
     5521 gtccggttca taggccaggc accagccgcc aaacacgaac agcgcctgta tcgaatagat
     5581 gacgaccacg gtctcgtggt gggtgaatcc cagccccagc agccgatggt ggatgtggtt
     5641 cttgctggcc ttgaaccagt tgttgccgct ggccgcacgc agatagaaca ctcccagaat
     5701 gtcgatcacc ggcagaccga tcagcagggc caccaccgcc ggactcaatg cactgttggt
     5761 gtactgggtc agacgcaccg ccagaaaggc cagcgtgaag ccgagaaact ggctgccgtt
     5821 gtcacccatg aacacctggg ccggatggct gttgaagcgc agaaaaccga gaatgccgcc
     5881 aatcaccgcc atggcgatca gcaaggtgat gctgcccccg gccagatagg ccagaaaggc
     5941 gatgacgatc agcgacagca gcacctcgcc ggcggcaagt ccatccagcc catcggcatg
     6001 gttgacgccg ttgatcaccc ccaccatcgc caccacggtg aacggaatgg ccaccgccgc
     6061 cggcagcgtc accgaatcga gcaacggcag atgctcgacc cgcagaccgc cataccagac
     6121 cagcggcacc accgccagaa actggccg
//
LOCUS       DAORCQ010000121         6143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_9254_length_6143_cov_5.488173, whole genome shotgun sequence.
ACCESSION   DAORCQ010000121 DAORCQ010000000
VERSION     DAORCQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9254_length_6143_cov_5.488173"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKI23_10355"
     CDS             <1..333
                     /locus_tag="PKI23_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNN37378.1"
                     /translation="RVWMRAVAPLPDALILHQCILAYASDRGLLGTALLPHAISIDNP
                     RLQAASLDHAMWFHRPFRADEWLLYVQESPSASNGRGFCLGHIYRSDGVLVASTAQEG
                     LIRHHPDR"
     gene            358..1329
                     /locus_tag="PKI23_10360"
     CDS             358..1329
                     /locus_tag="PKI23_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNN37379.1"
                     /translation="MKAILIDSAQRMQWRDTTTPQPAAGEVRIRVRATAVNRADLMQR
                     IGLYPPPAGASEILGLECAGEVEAVGAQVERWKPGDAVVALLAGGGYAEQVVCPADHL
                     LPLPKGLDFSEGAALPEVFATAWLNLFHEAALQPGERVLLHAGASGVGSAAIQLCRAF
                     GNPCFVTLGSAAKLQQCLALGAEAGHLRQQGSFVPAVKAWAAQGVDVVLDPVGGGYLA
                     DNLKVMAVDGRLLLIGLMGGASSELNLGLLLVKRFRLIGSTLRSRSDAAKATLLRDMG
                     ERLWPLFEAGRLRPIIHARCPIGEVEAAFALVESNQTFGKVVLELPG"
     gene            1425..2520
                     /gene="prfB"
                     /locus_tag="PKI23_10365"
     CDS             join(1425..1487,1489..2520)
                     /gene="prfB"
                     /locus_tag="PKI23_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015093725.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNN37380.1"
                     /translation="MEINPLLQRIADYRERGESLRGIFDYAEKADRLIEVNRELEAAD
                     VWNDPERAQALGRERATLEQIVNDCEALSHGLADNEELIELAASEDDEETLAAVGKDL
                     DQLGALLARMEFRRMFSGEMDSHNAYLDIQAGSGGTEAQDWANMLLRMYLRWAERKGF
                     KVELMEASPGEVAGLKSATLHLQGDHAYGWLRTETGVHRLVRKSPFDSGNRRHTSFAS
                     VFVSPEIDESIEIDINPADLRTDTYRASGAGGQHVNKTDSAVRITHLPSGIVVQCQSE
                     RSQHQNRAKAFDMLRARLYEQEIKERNAQKQALEESKADIGWGSQIRSYVLDASRIKD
                     LRTDVETANTGAVLDGDLDPFIEASLKMGL"
     gene            2554..4077
                     /gene="lysS"
                     /locus_tag="PKI23_10370"
     CDS             2554..4077
                     /gene="lysS"
                     /locus_tag="PKI23_10370"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNN37381.1"
                     /translation="MKNDTPPASHEEDGLDEHELIAQRKAKLAEWRQSGRAFPNQFRP
                     DALADDLNRLHADHDKAALTAAPVRVRVAGRMMLRRIMGKASFATLQDRSGRIQIYLR
                     NSLPDDSYEAFKRWDLGDIIGVEGVLFRTNSGELTIEVERAELLTKSLRPLPDKHKGL
                     VDQESRYRQRYLDLMVNEQTRRLFQIRSRLIEAIRHFMVRHDFIEVETPMMQVIPGGA
                     TARPFTTHHHALDMPLYLRIAPELYLKRLVVGGFERVFEINRNFRNEGLSTRHNPEFT
                     MIEFYLAYADYHDLMNLTETMLRTVAEQVLGTPRLHYQGIDIDLAQPFARMTLLEALI
                     RHNPELPAASFSDRDRLAGIVEELGIAVAAGWGLGKLQMELFEKSVEHRLLQPTFITC
                     YPTEVSPLARRSDSDPSVSDRFEFFVGGREIANGFSELNDPEDQAERFRSQVADKERG
                     DEEAMFYDEDYITALEYGLPPTAGEGIGIDRLVMLFTDSPSIRDVLLFPHMRPLERR"
     gene            4074..4778
                     /gene="ung"
                     /locus_tag="PKI23_10375"
     CDS             4074..4778
                     /gene="ung"
                     /locus_tag="PKI23_10375"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009042574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNN37382.1"
                     /translation="MNSAPPLQRVRLEASWKARLGAEFDAPYMVALRQFLLEQKQQGK
                     EIYPPGSEWFNSFELTPFDATRVVILGQDPYPGPGQAHGLCFSVRPGVEIPPSLHNIY
                     QELASDLGIEPASHGCLESWARQGVLLLNSVLTVERGRPGAHQGRGWERFTDRVIELL
                     NGHPRRLVFLLWGSQAQKKGEIIDPRRHCLLKAPHPSPLSAYRGFFGCRHFSKANDFL
                     IGQGESAIDWRLPPVG"
     gene            5338..>6143
                     /locus_tag="PKI23_10380"
     CDS             5338..>6143
                     /locus_tag="PKI23_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNN37383.1"
                     /translation="MSIFSHYRSRYEATQEEEFSLQEYLELCKRDPSVYASAAERMLI
                     AIGEPSLVDTSRDPRLSRIFSNKVIKTYPAFSEFYGMEEAIEQIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKSLMEKVPFYAIKGSPVNESPLGLFNPAEDGTILQED
                     YGIARQYLKGTMSPWAVKRLHEFHGDLSKFRVVKKYPSIHDQIGIAKTEPGDENNQDI
                     SSLVGKVDIRMLERFAQNDPDAYSYSGGLCNANQGLLEFVEMFKAPIKVL"
BASE COUNT         1118 a         2065 c         1988 g          972 t
ORIGIN      
        1 cgggtgtgga tgcgggcggt ggcgccgctg cccgatgcgc tgattctgca tcaatgcatc
       61 ctggcctacg cctcggaccg cggcttgctc ggcacggcgc tgctgcccca tgcgatctcg
      121 atcgacaatc cgcggctgca ggccgccagc ctcgaccatg cgatgtggtt ccatcggccc
      181 tttcgcgccg atgagtggct gctctatgta caggagagcc ccagcgccag caacggccgc
      241 ggcttctgcc tcggccacat ctaccgcagc gacggcgtgc tggtggcctc gaccgcccag
      301 gaggggctga ttcgccacca ccccgaccgc tgaccacccg atgatggagc agacaacatg
      361 aaagcgatcc tgatcgacag cgcacagcgc atgcagtggc gcgacaccac caccccacag
      421 ccggccgctg gcgaggtgcg catccgggtg cgggccaccg cggtcaaccg cgccgacctg
      481 atgcaacgga tcggcctcta tccgccgcca gccggggcca gcgagattct cggcctcgaa
      541 tgtgccggcg aggtcgaggc ggtcggcgct caggtcgaac gctggaagcc cggtgacgcg
      601 gtggtcgcgc tgctggccgg tggcggctat gccgaacagg tggtctgccc ggccgaccac
      661 ctgctgccgc tgccgaaggg gctggacttc agcgagggtg ccgcgctgcc ggaggtgttc
      721 gccaccgcct ggctcaacct gttccatgag gcggcgttgc agccgggcga gcgggtgctg
      781 ctccatgccg gtgccagcgg cgtcggcagc gcggcgatcc agctctgccg ggcgttcggc
      841 aacccctgct tcgtcacgct gggcagcgcg gcgaagctgc aacagtgtct ggcgctcggc
      901 gccgaggccg gccacctgcg ccagcagggc agcttcgtgc cggcggtgaa ggcgtgggca
      961 gcgcaggggg tcgatgtggt gctcgacccg gtcggcggcg gttacctcgc cgacaacctg
     1021 aaggtgatgg cggtggatgg ccggctgctg ctgatcggcc tgatgggcgg cgccagcagc
     1081 gagctcaacc tcggactgct gctggtgaag cgctttcgcc tgatcggctc gacgctgcgc
     1141 agccgcagcg acgccgccaa ggccacgctg ctgcgcgaca tgggcgagcg gctctggccg
     1201 ctgttcgagg cgggtcgatt gcggccgatc atccacgccc gctgcccgat cggcgaggtc
     1261 gaagcggcct ttgcgctggt cgaatcgaac cagaccttcg gcaaggtggt gctggagctg
     1321 ccgggctgag ccacggcact gcatcggcgg ccgctgattg ggtagaatgg ccgcccccgg
     1381 cgcttgcgcc actcctttta tatcttcaca ggcacccatc gatgctggaa atcaatccgc
     1441 tgctgcaacg cattgccgat taccgcgagc gcggcgagtc gctccggagg tatctttgac
     1501 tatgccgaga aggccgatcg gctgatcgag gtcaaccgcg agctggaagc ggccgatgtc
     1561 tggaacgatc cggagcgggc gcaggcgctc ggccgcgaac gcgccacgct cgagcagatc
     1621 gtcaacgact gcgaggcgct gagccacggg ctcgccgaca acgaagagct gatcgaactg
     1681 gccgccagcg aggacgacga ggagacgctg gcggcagtcg gcaaggatct cgaccagctc
     1741 ggcgcgctgc tggcgcggat ggagtttcgc cgcatgttca gcggcgagat ggacagccac
     1801 aacgcctacc tcgacatcca ggccggctcc ggcggcaccg aggcgcagga ttgggccaac
     1861 atgctgctgc gcatgtatct gcgctgggcc gagcgcaagg ggttcaaggt cgagctgatg
     1921 gaggcctcgc ccggcgaggt ggccggcctc aagagcgcca ccctccacct gcagggcgac
     1981 catgcctatg gctggctgcg caccgagacc ggcgtgcacc ggctggtgcg caagtcgccg
     2041 ttcgattcgg gcaaccgccg ccacacctcc ttcgcctcgg tcttcgtctc gccggagatc
     2101 gacgagagca tcgagatcga catcaatccg gccgacctgc gcaccgacac ctaccgcgcc
     2161 agcggcgccg gcggccagca tgtcaacaag accgattcgg ccgtgcgcat cacccacctg
     2221 cccagcggca tcgtcgtgca gtgccagagc gagcggtcgc agcatcagaa ccgcgccaag
     2281 gcgttcgaca tgctgcgcgc ccggctctat gaacaggaga tcaaggagcg caacgcgcag
     2341 aagcaggcgc tcgaagagag caaggccgac atcggctggg gcagccagat tcgctcctac
     2401 gtgctcgatg cctcccgcat caaggatctg cgcaccgatg tcgagaccgc caacaccggc
     2461 gcggtgctcg atggcgacct cgaccccttc atcgaagcca gcctgaagat gggactctaa
     2521 gccctgccga ccgccctcca ggagcactcc gcaatgaaaa atgacacgcc cccggccagc
     2581 cacgaagagg atggcctcga cgaacatgaa ctgatcgccc agcgcaaggc caagctggcc
     2641 gagtggcgcc agagcgggcg ggcctttccc aaccagttcc gtcccgacgc actggcggat
     2701 gacctgaacc gcctccatgc cgaccacgac aaggctgcgc tcaccgccgc gccggtgcgg
     2761 gtcagggtgg ccgggcggat gatgctgcgc cgcatcatgg gcaaggccag cttcgccacg
     2821 ctgcaggacc gcagcggacg catccagatc tacctgcgca acagcctgcc cgatgacagc
     2881 tacgaggcgt tcaagcgctg ggatctcggc gacatcatcg gcgtcgaggg ggtgctgttc
     2941 cgcaccaaca gtggcgagct gaccatcgag gtcgaacggg ccgagctgct gaccaagtcg
     3001 ctgcggccgc tgccggacaa gcacaagggg ctggtcgatc aggagagccg ctaccggcag
     3061 cgctacctcg acctgatggt caacgagcag acgcggcggc tgttccagat ccgctcgcgg
     3121 ctgatcgagg cgattcgcca cttcatggtg cggcacgact tcatcgaggt cgaaacaccg
     3181 atgatgcagg tgatccccgg cggcgccacc gcgcgcccct tcaccaccca tcaccatgcg
     3241 ctcgacatgc cgctctacct gcgcatcgcg cccgagctct acctgaagcg gctggtggtc
     3301 ggcggcttcg agcgggtgtt cgagatcaac cgcaacttcc gcaacgaggg gctgtcgacc
     3361 cgccacaacc ccgaattcac gatgatcgag ttctacctcg cctatgccga ctaccacgac
     3421 ctgatgaacc tgaccgagac gatgctgcgc acggtggccg agcaggtgct gggcacgcca
     3481 cggctgcatt accagggcat cgacatcgac ctggcgcagc cctttgcccg gatgacgctg
     3541 ctcgaagccc tgatccgcca caacccggag ctgccggccg ccagcttcag cgaccgcgac
     3601 cggctggccg gcatcgtcga ggagctgggc atcgcggtgg ccgccggctg ggggctcggc
     3661 aagttgcaga tggagctgtt cgagaagagc gtcgagcacc ggctgctgca accgaccttc
     3721 atcacctgct atcccaccga ggtgtcgccg ctggcgcgac gcagcgacag cgatccttcg
     3781 gtcagcgacc gcttcgagtt ctttgtcggc ggccgcgaga tcgccaacgg tttctcggag
     3841 ctgaacgacc ccgaagacca ggccgagcgg ttccgcagcc aggtggcaga caaggaacgc
     3901 ggtgacgaag aggcgatgtt ctacgacgag gattacatca ccgcgctcga atatgggctg
     3961 ccgccgaccg ccggcgaggg catcggcatc gaccggctgg tgatgctgtt caccgattcg
     4021 ccctcgatcc gcgacgtgct gctgtttccg cacatgcggc cgctggagag acggtgaaca
     4081 gcgcgccacc gttgcagcgg gtgcgcctcg aagcctcgtg gaaggcacgg cttggcgccg
     4141 agttcgacgc gccctacatg gtggcgctgc ggcagttcct gctcgaacag aagcagcagg
     4201 gcaaggagat ctatccgccc ggcagcgagt ggttcaacag ctttgagctg acgccattcg
     4261 acgccacccg ggtggtgatc ctcggtcagg acccctaccc cggaccgggg caggcccatg
     4321 gcctctgctt ctcggtgcgc cccggcgtcg agattccccc ctcgctgcac aacatctatc
     4381 aggagctggc cagcgacctc ggcatcgaac cggccagcca tggctgcctg gagtcctggg
     4441 cacggcaggg cgtgctgctg ctcaacagcg tgctgaccgt cgaacggggc cgacccggcg
     4501 cccaccaggg acgcggctgg gagcgcttca ccgaccgggt gatcgaactg ctgaacggcc
     4561 atccgcgacg gctggtcttt ctgctgtggg gcagtcaggc gcagaagaag ggagagatca
     4621 tcgacccccg gcgacactgt ctgctgaagg caccccaccc ctcaccgctg tcggcctacc
     4681 gtggtttttt cggctgccga cacttctcga aggccaacga ctttctcatc ggtcagggcg
     4741 agtcagcgat cgactggcgg ctgccgccgg tcggctgagt cgactcacac ccagccagcc
     4801 gcaacaaaag ggcgcacacc cggcactgtc acgccccaaa aaagggtagc ctgcaccatc
     4861 gatggcgggt tttacccgga ctgcgcacca ttttggccaa cctcggccgc aaaggaagca
     4921 ctgatttatt cgtcgtgcca ttcctcccag cccctcaccc ccggcccctc tcccgcatgt
     4981 gggagagggg tgcgcagcag cgcgggtgac cgaatgggtc atatctgccc accaccgtca
     5041 tttgattgac atctgacgaa gcgcacgccg gcaatcggtg acggaaacgg caattttcgt
     5101 caactttccg gctggcaccg caccacaccc gaggcgcacc gtgaaaagcg ccaggacaac
     5161 ccagccattc actcgggatt ccgcgccctg cgcctcactg cttcgcaaca tggcctacag
     5221 cttgcagagc tattgacaag cgcgttcggt gtggtgttgc cgcccagaac ggcagactca
     5281 agtgcccgca gaaggttcga ctgattttca acgttcaccc aaggcgaggt tgcagcgatg
     5341 tcgatattca gccactaccg ctcccgttac gaggcgacgc aggaggagga gttcagcctg
     5401 caggagtacc tcgaactgtg caagcgcgac ccttccgtct acgccagcgc cgcggagcgc
     5461 atgttgatcg ccatcggcga gccgagcctg gtcgacacca gccgggatcc gcgactgagc
     5521 cggatcttct ccaacaaggt gatcaagacc tacccggcct tttcggagtt ctacggcatg
     5581 gaggaggcga tcgagcagat cgtctcctac ttccgccatg ccgcccaggg gctggaagag
     5641 aagaagcaga ttctctacct gctggggccg gtcggtggcg gcaagtcctc gctggccgag
     5701 aagctgaagt cgctgatgga gaaggtgccc ttctatgcga tcaagggttc accggtcaac
     5761 gaatcgccgc tcgggctgtt caaccccgcc gaggatggca ccatcctgca ggaggattac
     5821 ggcatcgcgc gccaatatct gaaagggacc atgtcgccgt gggcggtgaa gcggctgcat
     5881 gagttccatg gcgacctcag caagttccgg gtggtgaaga agtatccgtc gatccatgac
     5941 cagatcggca tcgccaagac cgaaccgggc gacgagaaca accaggacat ctcctcgctg
     6001 gtcggcaagg tcgacatccg catgctggag cgctttgccc agaacgaccc ggatgcctac
     6061 tcctactccg gcggactgtg caacgccaac caggggctgc tcgaattcgt cgagatgttc
     6121 aaggcgccga tcaaggtgct gca
//
LOCUS       DAORCQ010000122         5531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_10787_length_5531_cov_6.720964, whole genome shotgun sequence.
ACCESSION   DAORCQ010000122 DAORCQ010000000
VERSION     DAORCQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10787_length_5531_cov_6.720964"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /gene="miaB"
                     /locus_tag="PKI23_10385"
     CDS             complement(<1..1180)
                     /gene="miaB"
                     /locus_tag="PKI23_10385"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNN37384.1"
                     /translation="MNKKLYIQTHGCQMNEYDSSRMGDLLAASHQLELTSNPEEADVL
                     LLNTCSIREKAEEKVFHQLGRWKRLKQQRPELIIGVGGCVASQEGAALRKRAPHVDLV
                     FGPQTLHRLPEMIDRQRRALLPVVDVSFPEIEKFDLLPDPGANGPTASVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDDVVAEVFHLAQQGVREVTLLGQNVNAYRGRTHQGELADL
                     AELLHYVAAIDGIERIRFTTSHPLEMSDSLIAAYATLPKLAPHLHLPVQSGSDRILAR
                     MKRNYTAADYLAIIDRLKAACPDIQLSSDFIVGFPGEEEADFEATLDLIRQVGFDQSF
                     SFIYSPRPGTPAAELPDSVPLEVKGERLARLQALINQQAQAISAAMLGSQQSILVSGP
                     A"
     gene            complement(1249..2334)
                     /locus_tag="PKI23_10390"
     CDS             complement(1249..2334)
                     /locus_tag="PKI23_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HNN37385.1"
                     /translation="MKAALYRGSDKPIVVEEIEIDAPEGSEVLVDLNACGVCHSDLHV
                     MGMMAQPPVPMVLGHEAAGVVRAVGKDVTSVAAGDHVIVAFRGACGKCFYCVKGMRQL
                     CANADLPDRQVTGPRPRLKQNGVPIVQGVSVGGWAHQVLLPETSMVKIRKDAPLDKVS
                     LIGCGVMTGIGAAIHTAKVEPGSDVAVIGCGGVGLNVIQGAKLAGAHRIIAVDLLPNK
                     LEMAREFGATHCVAAGEGDPVAKVKEISPYLDYAFEVIGLPVTAQQAFAMIRPGGTAV
                     VVGVGFGRVAEIPLGDFLQEKRLVGSFYGSSQVHVDIPRLVDLYMEGKIQLDPLVSQV
                     RPLSEVNEALKAMKAGEVARTLLSMSL"
     gene            2478..3314
                     /locus_tag="PKI23_10395"
     CDS             2478..3314
                     /locus_tag="PKI23_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNN37386.1"
                     /translation="MSPPPVRQWQARLDIHLNRQGARTVLAAHRHSGPLRLQRPFYPE
                     GSAVCHLCLLHPPGGLVMGDELSTTLTLEAAAEAVVTTPSAGKFYGVGDGPAQRQCIE
                     LNVGEQATLEWLPQENIYYAGANAQTRTRVRLAAGASFVGWEIICLGRPAANAPFVAG
                     RLDQQWELWRDERPLFIERLRLAADDPLVTHRSGLQGLPVCGTLIATFPEHLPLPAWQ
                     AQWHQDDSPIVLTRIDELLVARYLGHSTEQARLLFGALWQAVRPLLPSGRTALPPRIW
                     NC"
     gene            3339..3641
                     /gene="ureA"
                     /locus_tag="PKI23_10400"
     CDS             3339..3641
                     /gene="ureA"
                     /locus_tag="PKI23_10400"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNN37387.1"
                     /translation="MELTPREKDKLLLFTAALLAERRKARGLRLNYPEAVAYISAAIL
                     EGARDGRSVAELMASGRTLLSRDEVMEGVPEMIREVQVEGTFPDGTKLVTVHDPIP"
     gene            3712..4932
                     /locus_tag="PKI23_10405"
     CDS             3712..4932
                     /locus_tag="PKI23_10405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNN37388.1"
                     /translation="MRLLLHLLESALDHGRLTLIDPRGHPHRLGPGGPPEVTVRLHDR
                     ALPRRLLWQTELAAGEAYMAGRLTVEQGTLRDFLALATGCATALGHTGWQRLLHRIER
                     PLRWLHQFNPLRRARRNVAHHYDLSHELYALFLDADLQYSCAYFARGDETLEQAQALK
                     KRHIAAKLLLTPGMRVLDIGCGWGGLALDLARHDGVVVTGLTLSSEQLQWAQSRAQDA
                     GLAEQVQFALRDYREEQGRYDRIVSVGMFEHVGVDHYRHFFRALGRMLATDGVALVHA
                     IGRVDPPGGTNAWTRKYIFPGGYCPALSEVLVAVEKSGLWVTDLEILRLHYAETLRHW
                     FERFQARRERARALYDERFCRMWEFYLASAEMAFRHGRLMVFQLQLAHRPDAVPLTRD
                     YLCQPIPVWDHDSG"
     gene            5041..5358
                     /locus_tag="PKI23_10410"
     CDS             5041..5358
                     /locus_tag="PKI23_10410"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012272563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNN37389.1"
                     /translation="MIPGELDPASGDIELNAGRPTLRLTVANSGDRPIQVGSHYHFAE
                     TNPALLFDRAAARGHRLDIAAGTAVRFEPGQSRTVELVALAGTRRVYGFNGEVMGELE
                     AAP"
     gene            5355..>5531
                     /gene="ureC"
                     /locus_tag="PKI23_10415"
     CDS             5355..>5531
                     /gene="ureC"
                     /locus_tag="PKI23_10415"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093747.1"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNN37390.1"
                     /translation="MSRIDRRAYAEMYGPTRGDRLRLGDTALWIEVERDFTVYGEEVK
                     FGGGKVIRDGMGQSQ"
BASE COUNT          913 a         1810 c         1845 g          963 t
ORIGIN      
        1 tggccggccc actgaccaga atcgactgtt ggctgccgag catcgcggcg ctgatcgcct
       61 gggcctgctg attgatcagc gcctgcagcc gcgccagacg ctcgcccttg acctccagcg
      121 gcaccgagtc gggcagttcg gcggccggcg tgccgggacg cgggctgtag atgaagctga
      181 atgactgatc gaagccgacc tggcgaatca ggtcgagcgt ggcctcgaag tcggcctcct
      241 cctcgccggg gaagccgacg atgaagtcgg aggagagctg aatgtccggg caggccgcct
      301 tcagccggtc gatgatggcc agatagtcgg ccgccgtgta gttgcgcttc atccgcgcca
      361 ggatgcggtc ggagccgctc tgcaccggca ggtgcagatg aggcgccagc ttgggcagtg
      421 tcgcgtaggc ggcgatcagg ctgtcgctca tctccagcgg atgtgaggtg gtgaagcgga
      481 tgcgctcgat gccgtcgatg gcggcaacgt agtgcagcag ctcggccaga tcggccaact
      541 cgccctggtg ggtgcggccg cggtaggcat tgacgttctg gcccagcagc gtgacctcgc
      601 gcacgccctg ctgcgccaga tggaacacct cggccaccac gtcgtcgaac ggccggctga
      661 tctcttcgcc gcgggtgtag ggaacgacgc agaacgagca gtacttgctg cacccctcca
      721 tgatcgagac cgaggcggtc ggcccattgg cgccgggatc gggcagcagg tcgaacttct
      781 cgatctcggg gaaactgaca tcgaccaccg gcagcagcgc cctgcgctgc cggtcgatca
      841 tctccggcaa tcgatgcagt gtctgcgggc cgaacaccag gtcgacatgg ggtgcccgct
      901 tgcgcagtgc cgcgccctcc tggctggcga cgcagccacc gacgccaatg atcagctcgg
      961 gtcgttgctg cttcagccgc ttccagcggc ccagttgatg aaagaccttc tcttcggcct
     1021 tctcgcggat cgaacaggtg ttgagcagca gcacatcggc ctcttcggga ttgctggtca
     1081 gctcgagctg atggctggcg gcgagcagat cgcccatgcg ggacgagtcg tactcgttca
     1141 tctgacagcc gtgggtctga atgtacagct tcttgttcat caccggccgg gttcgtcaaa
     1201 aaaaggggcc gcgacccgcc attgcggcag gtcgcggcct ggggtcgatc aaagcgacat
     1261 cgacagcagc gtgcgcgcca cttcacccgc cttcatcgcc ttgagggctt cgttcacttc
     1321 gctgagtggg cgaacctgcg agaccagcgg gtcgagctgg atcttgcctt ccatgtagag
     1381 gtcgaccagc cggggaatgt cgacgtggac ctggctcgaa ccgtagaagg agccgaccag
     1441 acgcttctcc tgcaggaagt cgccgagcgg aatctcggcc acgcggccga agccgacacc
     1501 caccaccacc gcggtgccgc ccgggcggat catcgcgaag gcctgctgtg cggtgaccgg
     1561 cagaccgatc acctcgaagg cgtagtcgag gtaggggctg atctccttca ccttggccac
     1621 cggatcaccc tcgccggcgg cgacgcagtg ggtggcaccg aattcacgcg ccatctccag
     1681 cttgttcggc agcagatcga ccgcgatgat gcggtgtgca ccagccagct tggcgccctg
     1741 gatgacgttg aggccgacac caccgcaacc gatcaccgcg acatcgctgc ccggctccac
     1801 cttggcggtg tggatggcgg caccgatgcc ggtcatcaca ccgcagccga tcaggctcac
     1861 cttgtcgagt ggagcatcct tgcggatctt caccatgctg gtctccggca gcagcacctg
     1921 gtgggcccag ccaccgaccg agacgccctg cacgatcggc acgccattct gtttcaggcg
     1981 cggacgcggg ccggtcacct gccgatcggg caggtcggcg ttggcgcaga gctggcgcat
     2041 ccctttcaca cagtagaaac acttgccgca ggcgccacgg aaggcgacga tgacatggtc
     2101 gcccgctgcc acgctggtca catccttgcc caccgcacgc acgacgccgg cggcttcgtg
     2161 gcccagcacc atcgggaccg gcggctgcgc catcatgccc atcacatgca ggtcactgtg
     2221 gcagacacca caggcattca gatcgaccag aacttcactg ccttcgggcg cgtcgatctc
     2281 gatctcctcg acgacgatcg gcttgtcact gccacggtaa agggctgctt tcatcggatt
     2341 tctccttggt tattatgtcg ttgaatcaat gaaggaacaa aggaacggtt ggtttggaaa
     2401 ctatatcata tcaggctgac ttgaacggtt gcagcgcggc ggcacctgct gccgatcgcc
     2461 acggcggcac ttcgtcgatg tcaccccctc cggtgcgcca atggcaggcc cggctcgaca
     2521 tccatctgaa ccgccagggc gcccgcaccg tgctggccgc gcaccggcac agcgggccac
     2581 tgcggctgca gcggccgttc tatcccgagg gcagtgcggt ctgtcacctc tgcctattgc
     2641 atccgccagg cgggctggtg atgggcgacg agctgtcgac cacgctcacg ctcgaagccg
     2701 ctgccgaagc cgtggtgacc acgccgtcgg cgggcaagtt ctatggcgtc ggtgacggcc
     2761 cggcacagcg gcaatgcatc gagctgaatg tcggcgagca ggccacgctg gagtggctgc
     2821 cgcaggagaa catctactac gccggcgcca atgcccaaac tcgcacccgg gtcaggctgg
     2881 cggccggtgc cagtttcgtt ggctgggaaa tcatctgcct gggtcgcccg gcggccaatg
     2941 caccctttgt cgccggccgc ctcgatcagc agtgggagct gtggcgcgac gagcggccgc
     3001 tcttcatcga acggctgcgg ctggcggccg atgatccgct cgtcacccac cgcagcgggc
     3061 tgcaggggct gccggtctgc ggcaccctga ttgctacctt ccccgaacat ctgccgctgc
     3121 cggcgtggca ggcgcagtgg caccaggatg acagcccgat cgtgctgacc cgcatcgacg
     3181 aactgctggt ggcacgctat cttggtcact cgaccgaaca ggcccgactg ctgttcgggg
     3241 cgctctggca ggccgtgcgg ccactgctgc ccagcggacg cacggcgctg ccgccaagaa
     3301 tctggaactg ctgacaccct ctgctgtcga gatcgaccat ggaactgacc ccgcgcgaaa
     3361 aagacaaact gctgctgttc accgcggcgc tgctggccga gcggcgcaag gcacgcggtc
     3421 tcaggctcaa ctaccccgaa gcggtggcct acatcagcgc cgccattctc gaaggtgccc
     3481 gcgatggccg cagcgtggcc gaactgatgg cgagcgggcg caccctgctg agccgtgacg
     3541 aggtgatgga gggggtgccg gagatgatcc gggaggtgca ggtcgagggc acctttcccg
     3601 atggcaccaa gctggtgacg gtgcacgatc cgattccgtg actggcaagc cggcggtgag
     3661 tcgaccgctg gcgccagcag aatcctgttc cgtttgatca ggaggtgtgc gatgcggctg
     3721 cttcttcatc tgctcgaatc ggcgctcgac catggccgac tgaccttgat cgatccacgc
     3781 ggccaccccc atcgactcgg tccgggcgga cctcccgagg tcactgtgcg gctgcatgac
     3841 cgggcgctgc cgcgccgcct gttgtggcag actgaactgg cggcgggcga ggcctacatg
     3901 gccggacggc tgacggtcga gcagggcacg ctgcgcgatt ttctggcgct ggcgaccggc
     3961 tgtgccacgg cgctgggcca caccggctgg caacggctgt tgcatcggat tgagcggccg
     4021 ctgcgctggc tgcaccagtt caacccgctg cggcgcgcac gccgcaacgt ggcccaccac
     4081 tacgacctgt cacatgagct ttacgcgctc ttcctcgatg ccgacctgca gtactcctgc
     4141 gcctatttcg cgcggggcga tgagacgctg gagcaggccc aggcgctgaa aaaacgccat
     4201 atcgccgcca agctgctgct gacaccgggg atgcgggtgc tggacatcgg ctgtggctgg
     4261 ggtggcctgg cgctcgatct ggcgcgccat gatggggtgg tggtgaccgg actgaccctc
     4321 tccagtgagc agttgcagtg ggcgcagtcg cgggcacagg atgccggtct ggctgagcag
     4381 gttcagttcg cgctgcgcga ctaccgcgag gagcagggcc gctatgaccg catcgtctcg
     4441 gtggggatgt tcgagcatgt gggcgtggac cactaccggc acttttttcg tgcactcggc
     4501 cggatgctgg ccaccgatgg cgtcgcgctg gtgcatgcca tcggccgggt cgatccaccg
     4561 ggcggcacca atgcctggac ccgcaaatac atctttcccg gcggctactg cccggcgctc
     4621 tccgaagtgt tggtggcggt ggaaaagagc ggcctgtggg tgaccgatct ggagatcctg
     4681 cggctgcact atgccgagac gctgcgccac tggttcgagc gcttccaggc gcggcgtgaa
     4741 cgtgcccggg cactctatga cgagcgcttc tgtcgcatgt gggagttcta tcttgccagt
     4801 gccgagatgg cctttcgcca tggccggctg atggtgttcc agcttcagct cgcccaccgc
     4861 cccgatgcgg tgccgctgac ccgcgactac ctctgtcaac cgataccggt ttgggatcac
     4921 gacagtggct gaaacatccg ggcagattct ggcgtggcgg ttgccttggc ttatgatggc
     4981 atcgcactgc catgcggtca cgatcgaccg cgctgccgcc aaccttgcca ggagagcccg
     5041 atgattccag gagaactcga tcccgccagc ggcgacatcg agctcaatgc cggccggccg
     5101 acgctgcggc tgacggtggc caacagtggc gaccggccga ttcaggtcgg ttcgcactac
     5161 cactttgccg agaccaatcc ggcgctgctg ttcgaccgcg ccgccgcgcg cggccaccgc
     5221 ctcgacatcg ccgccggcac cgccgtgcgc ttcgagccgg gccagagtcg cacggtcgaa
     5281 ctggtggcgc tggccggcac gcggcgcgtc tacggcttca atggcgaggt gatgggtgaa
     5341 ctggaggcgg cgccatgagc cgcatcgacc ggcgcgccta tgccgaaatg tatggcccca
     5401 cccgcggtga ccggctgcgc ctgggtgaca cggcgctgtg gatcgaggtg gagcgcgact
     5461 tcacggttta tggcgaggag gtgaagttcg gcggcggcaa ggtgatccgc gacggcatgg
     5521 gccagagcca g
//
LOCUS       DAORCQ010000123         5407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_11160_length_5407_cov_4.649103, whole genome shotgun sequence.
ACCESSION   DAORCQ010000123 DAORCQ010000000
VERSION     DAORCQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11160_length_5407_cov_4.649103"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKI23_10420"
     CDS             <1..495
                     /locus_tag="PKI23_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN37391.1"
                     /translation="DEGAVIILDLPALLRAKPEGHATSSSLASTHGHDAQTCLVVDDS
                     VTMRKVTSRLLERQGYRVLMARDGVEALAMLNESRPDVMLLDVEMPRMDGFELVSRIR
                     HQSGLEDLPIIMITSRTGAKHRERARELGITHYLGKPYAEQALLLAIDTVTTTAAYPE
                     QRCS"
     gene            492..998
                     /locus_tag="PKI23_10425"
     CDS             492..998
                     /locus_tag="PKI23_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNN37392.1"
                     /translation="MTTRPSPNVTLLDLVVLPLTQRQLLLPASALAEVLPKWQSRPLP
                     ETPQWCAGELEWRQRRLPLIDFERWSGGPDTPGRWLVVCNRSQDDLPFSHYGVLLAGR
                     PRLLRLRSNQLRGIPTPSDAGIGAMTLVEGERYAIPDLMALERQIDQAMRGTRLREAA
                     GQSPVKPD"
     gene            1074..2324
                     /locus_tag="PKI23_10430"
     CDS             1074..2324
                     /locus_tag="PKI23_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNN37393.1"
                     /translation="MNLPTIDLSTMPAVQATAQSTVMISSESPNAAPTGDGRSATADF
                     QALMAALQGQLGPMNAAGATQSGAMTTKQGTVQPQQPSSEPLLTLVEETLTEATAGGT
                     VASEENMQPAQSDKEPSGDGLSLPFIGTLLPPTTPLTTTALAVERQANENEASTATDP
                     ASLLRFESGMQKTSEPTHSSGLLSETSQSGAERQFMDELKNLLAMMPGAGHEDPAPTA
                     DAQAVTPLAQSIGSASPDGTANLTGTAMTSLAPPFLHNSPGDTAMTGKAELPPARLLM
                     EQPLNLDGDTWVDGFAERILWASKNQFHYAEIQLDPPELGALQVRIQIHQDQAQVQFI
                     SPHHQVREAIEGSLDRLRDLFQQQGMQLAHSQVADGGGRGSAQQESRREGWHPIAGEN
                     PATTEEPMLQSRPLREGALIDRYA"
     gene            complement(2380..2505)
                     /locus_tag="PKI23_10435"
                     /pseudo
     CDS             complement(2380..2505)
                     /locus_tag="PKI23_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2706..3200
                     /locus_tag="PKI23_10440"
     CDS             2706..3200
                     /locus_tag="PKI23_10440"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNN37394.1"
                     /translation="MAPVPALAVQYMTTEAAQKLMFPEANLFIPHFVRLDDAQKEAVE
                     SATGVKMRHAEQPVWQVMVGGKPTGWFVRDEVYGKHEFITYAVALDASGAVRGVEILD
                     YRETHGREIINPKWRAQFTGKRHGATLKLGDDVQNISGATLSCKHITEGVRRVLAIHQ
                     AVLK"
     gene            3197..4117
                     /locus_tag="PKI23_10445"
     CDS             3197..4117
                     /locus_tag="PKI23_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNN37395.1"
                     /translation="MKVRRAQPWLGTLVDIQVEGDDAMRLRSACDDAFGRIAEIHAAL
                     SFQSPDSELTHVNRMAQTDWVGLSPDFTAVLAAALDFARLGDGNFDPCVGGRLVAAGR
                     LPRHAGFPEKTNGNWRAIEFDGERLRFRAPLLLDFSGIAKGHAVDQALATLRSTGVAA
                     ATVNAGGDLAHFGHTPIPFELRDPTHPTRLLRLAQITCGAAATSADYFQPGQLIDPAD
                     GTPLCRASSVTVLAADCLTADALTKIVAADPKHAPARLAHHGAQAILFDAEGLHLGDA
                     KGWRTVYAAVRSATPIAGHGQRPQPVTALN"
     gene            4130..5338
                     /locus_tag="PKI23_10450"
     CDS             4130..5338
                     /locus_tag="PKI23_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37396.1"
                     /translation="MKTHLAPTLLAASLAATLALPTLAADADTEARLKALEARLNALE
                     AENQLLKRQQKATEQRVDTVQEEAKPSGSAGWAENSRIGGYGELHYNNLDGEGGASDK
                     EEIDLHRFVLFFGHQFNERTRFFSELELEHSISGEGKKGEIELEQAYVEFDLNTHHRA
                     KAGLFLLPIGLINETHEPPVFYGTERNPIEREIIPATWWAGGTGLSGDLGSGFGYDIA
                     IHEGLATTAAKGYKPRDGRQKTSEARAEEIAYTARLKWSGLPGLSLAAAFQHQSDITQ
                     NLDATAGSANLFEVDGVFNHGPFTLKALYARWTLEGSGPAAIGADEQFGGYIEPSFKL
                     NEQWGLFARYNLWDNAAGAGTASEKRQTDAGVNYWPHPDVVLKADYQWQDNDDDKNQN
                     GFNLGVGYQF"
BASE COUNT         1115 a         1767 c         1605 g          920 t
ORIGIN      
        1 gatgagggcg cggtgatcat tctcgacctg cccgccctgc tgcgcgccaa gcctgaaggc
       61 catgccacct ccagcagtct ggccagcact cacggccacg atgcgcaaac ctgcctggtg
      121 gtggatgatt cggtcaccat gcgcaaggtg acctcacgcc tgctcgaacg acagggctat
      181 cgggtgttga tggcacgtga cggtgtcgag gccctggcga tgctgaatga gagccgtccg
      241 gatgtcatgc tgctcgatgt cgagatgccg cggatggatg gcttcgaact ggtcagccgc
      301 attcgtcacc agagtggtct ggaagatctg ccgatcatca tgatcacctc ccgcaccggc
      361 gccaaacacc gcgaacgggc ccgcgaactc ggcatcaccc actatctggg caagccttac
      421 gccgaacagg cgctgctgct cgccatcgat acagtgacca caacagcggc ctatccggaa
      481 cagcgctgct catgacgaca cggcccagtc ccaacgtcac gctgctcgat ctggtggtgt
      541 tgccactgac gcagcgccaa ctgctgctgc cggccagcgc gctggccgaa gtgttgccca
      601 agtggcaaag ccgtcccctg cctgaaacgc cgcagtggtg cgcgggggag ctcgaatggc
      661 gccaacgccg actgccactg atcgacttcg aacgctggtc gggcgggccg gacacaccgg
      721 gcagatggct ggtcgtctgc aaccgcagtc aggacgatct gccgttcagc cattatggag
      781 tgctgctcgc cggacgcccc cggctattgc ggctgcgcag caatcagctc cggggcattc
      841 ccacgccatc cgatgccgga atcggtgcca tgacactggt ggagggtgaa cgctatgcca
      901 tccccgatct gatggcactc gagcgacaga tcgaccaggc gatgcgtggt acccggctgc
      961 gcgaggccgc cggtcagtca cccgtcaaac cggattgagc cgagcgccgc gcactcccga
     1021 tttctggcaa tgatcttgct gtgtacgtcc aatcgtcact gcaaggagtc gagatgaatc
     1081 tgcccaccat cgacctgtcc accatgccgg cggtgcaggc cactgcacaa agcaccgtca
     1141 tgatcagctc agagtcgccg aatgccgccc cgactggcga tggccgcagc gcaacggctg
     1201 attttcaggc cctgatggct gcattgcagg gtcagctcgg cccgatgaac gcggctggcg
     1261 ccacccaatc tggggccatg acaacgaaac agggcacagt ccaaccgcag cagccaagct
     1321 cggaaccgct gctgacgctg gtggaagaga ccctcactga agccactgcg ggtggcacag
     1381 tcgcctccga agagaacatg caacctgcgc agagcgacaa ggagccgtcg ggcgacggtc
     1441 tgagcttgcc gttcatcggc acactcttgc cgccgacaac gccgctgaca accacagcat
     1501 tggccgttga acgccaagcc aatgaaaatg aagcaagcac ggccaccgat ccggcctctc
     1561 tgttacgatt tgaaagcgga atgcagaaga cctccgagcc gactcattcg agcggtctgc
     1621 tgagcgaaac cagtcaaagt ggggccgaac ggcaattcat ggacgaactg aaaaacctgt
     1681 tggcgatgat gcccggcgcc ggtcatgaag acccggcacc gaccgccgat gcccaggctg
     1741 tcacaccgct ggcgcaatcc atcggctccg ccagccctga tgggacggcc aatctgactg
     1801 gaacggccat gacgtcgctg gcaccgccct tcctgcacaa ttcacctggc gacaccgcaa
     1861 tgacaggcaa ggctgagttg ccacccgcca ggctcctgat ggagcagccg ctcaacctcg
     1921 atggcgatac ctgggtcgat gggtttgccg agcgcattct ctgggcaagc aagaaccagt
     1981 tccactatgc agagattcag ctcgacccgc ccgagctggg cgcactgcag gtgcgcattc
     2041 agatccatca ggatcaggcg caggtgcagt tcatcagccc ccaccaccag gtgcgcgaag
     2101 ccatcgaagg cagcctcgac cgactgcgcg atctgtttca gcagcaaggg atgcaactgg
     2161 cccacagcca ggtcgccgat gggggtggcc gcggcagcgc ccaacaggag tcacgccgtg
     2221 aaggttggca tcccattgca ggtgaaaacc cagccaccac cgaagagccc atgcttcagt
     2281 cacgcccact gcgcgaaggc gcactgatcg accgttatgc ctgaggggca atgaacttgc
     2341 ccgccatcgc cggatacatt gccaagcggc tttggccgcc tcgaacgctt ccgggctgag
     2401 atagccgttg gcgctgtgcc ggcggattcg attgcaatca acctcgatgt actcgaacac
     2461 ggtcgcgcgc agctgcctgc gcttggcaaa ccgctcgccg tggactcgaa aggaggtttt
     2521 ccatccattt caatgacagc ccatggctga gattgccatg catataagaa cagattttat
     2581 ttgtattcaa gaatgattct tgtttatatt gacggcaagc aaacggcaaa acagcttttg
     2641 aggcagatca cgtcgtgtcg caaactccaa actggctgat tttttccgct gcctgtgtgc
     2701 cgctcatggc gccggtgcct gcgcttgccg tgcagtacat gacgacggag gcagcgcaaa
     2761 agctgatgtt tccggaagca aacctgttca tcccgcattt cgtgcgactc gacgatgcgc
     2821 aaaaggaagc ggttgagagc gccaccggcg tgaagatgcg ccacgccgag cagccggtct
     2881 ggcaggtgat ggtgggtggc aaacccaccg gctggttcgt gcgggacgag gtgtacggca
     2941 agcacgagtt catcacctac gccgttgcgc tcgatgccag cggcgcggtg cgtggcgtcg
     3001 agattctcga ctaccgcgag acccatggcc gcgaaatcat caacccgaaa tggcgcgcgc
     3061 agttcaccgg aaaacgtcat ggcgcgacgc tgaagctcgg tgacgacgtc cagaacatca
     3121 gtggcgccac gctctcgtgc aaacacatca ccgagggtgt gcgccgcgtg ctggcgattc
     3181 atcaggcggt gctgaagtga aggtgcgccg cgcacagccc tggctcggca cgctggttga
     3241 tatccaggtc gaaggcgatg acgcaatgcg actgcgcagc gcgtgtgatg acgccttcgg
     3301 gcgcattgcc gaaatccacg cggcgctgag ctttcagtca cccgattccg agttgacgca
     3361 cgtcaaccgc atggcacaaa ccgactgggt gggtctgtca cccgacttca ccgccgtgct
     3421 cgcagccgca ctcgacttcg cccggcttgg cgacggcaat ttcgaccctt gcgtcggtgg
     3481 gcggctggtt gcagcaggcc ggctgccacg ccacgctgga tttcctgaaa agaccaatgg
     3541 caactggcgc gcaatcgagt tcgacggcga acgcctgcgt tttcgggctc cactgctgct
     3601 cgatttttcg ggcatcgcca aaggccatgc ggtcgatcag gcgctcgcca cgctccgcag
     3661 caccggcgtc gctgccgcca ccgtcaacgc cggtggcgat cttgcacatt tcggccacac
     3721 gccgattcca ttcgagctgc gcgatcccac acaccctacc cgcctgctgc ggctcgcgca
     3781 gatcacatgc ggtgccgctg cgacctcggc cgattacttc caacccggcc agctgattga
     3841 tccggcggat ggcacaccgc tgtgccgcgc atcaagcgtc accgtgctcg ctgccgactg
     3901 cctcaccgcc gacgccctga ccaagatcgt tgccgcagac ccgaaacacg cgcccgcacg
     3961 gctcgcccac catggcgcac aagcgatcct gttcgatgcc gagggtctgc acctcggcga
     4021 tgcgaagggc tggcgaactg tgtatgccgc cgttcgttct gccactccaa tcgccggcca
     4081 cgggcaacgt ccccagccgg tcaccgctct caactgacct ggagttctga tgaaaaccca
     4141 ccttgccccc accctgctcg cggcttcgct tgccgccaca ctggcgctgc ccacgctggc
     4201 cgccgacgcg gacaccgaag cacggttgaa agcactggaa gcgcgcctca atgcgcttga
     4261 ggccgaaaat cagctgctga agcgtcagca aaaggccacc gagcagcgag tcgacaccgt
     4321 tcaggaagag gccaaacctt ccggaagcgc cggctgggcg gaaaacagcc gcatcggcgg
     4381 ttatggtgag ttgcactaca acaacctcga cggcgaaggc ggggcgtccg acaaggaaga
     4441 gatcgacctg caccgctttg tgctgttctt cggccaccaa ttcaatgaac gcacgcgttt
     4501 cttctctgaa ctcgaactcg aacacagcat ctccggggag ggcaagaaag gcgagatcga
     4561 actcgaacag gcctacgtcg agttcgacct caacacccat caccgcgcca aggccggtct
     4621 gttcctgctc ccgatcggcc tcatcaatga aacgcatgag ccaccggtgt tttatggcac
     4681 cgagcgcaat ccgatcgaga gagagatcat ccccgccact tggtgggccg gcggcaccgg
     4741 cctgtcaggc gacctcggct ccggtttcgg ctacgacatc gccattcacg aggggcttgc
     4801 caccacggcg gcgaagggct acaagccacg cgatggccgc cagaagacca gcgaggcgcg
     4861 tgccgaggag atcgcctaca ctgcacgcct gaagtggagc ggcctgcccg ggctgtcact
     4921 ggctgccgcc ttccagcatc agagcgacat cacccagaac ctggacgcaa ccgccggcag
     4981 cgccaacctg tttgaagtgg atggtgtgtt caaccacggc cccttcaccc tcaaggcgct
     5041 gtacgcgcgc tggactctcg aaggcagcgg tccggccgcg atcggcgcgg atgagcagtt
     5101 cggcggctac atcgaaccct cattcaagct caacgaacag tggggcctgt tcgcacgcta
     5161 caacctgtgg gacaacgcgg ctggcgccgg gacggccagc gaaaaacggc agaccgatgc
     5221 cggggtgaac tactggccac accccgatgt ggtgctcaag gctgactacc agtggcagga
     5281 taacgacgac gacaaaaacc agaacggttt caacctcggt gtcggctacc agttctgatc
     5341 catctgccca gcggccggct caataatcaa gtgcaacacc ttgttaaggt gttgcgatgg
     5401 gaaacga
//
LOCUS       DAORCQ010000124         5314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_11456_length_5314_cov_6.165431, whole genome shotgun sequence.
ACCESSION   DAORCQ010000124 DAORCQ010000000
VERSION     DAORCQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11456_length_5314_cov_6.165431"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PKI23_10455"
     CDS             <1..301
                     /locus_tag="PKI23_10455"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37397.1"
                     /translation="ADAQGMVTVNSTAGLQALRMQKPVQVLGSAVYDIAGLTHQAGLD
                     RFWTAPGAPDPLLLDAFIRLLVQRTQLRGVFFAPSAQHGAVIDRFAERLLSLPPA"
     gene            complement(312..653)
                     /locus_tag="PKI23_10460"
     CDS             complement(312..653)
                     /locus_tag="PKI23_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNN37398.1"
                     /translation="MNCLFCKIASGEISTQLVHEDDEIVAFNDINPQAPQHILLIPRR
                     HITTLNDLEADDTLLMGRMIQTATQLAKRLGFADTGFRTLFNCNQQGGQSVYHIHLHL
                     LAGRQMRWPPG"
     gene            853..1356
                     /gene="tadA"
                     /locus_tag="PKI23_10465"
     CDS             853..1356
                     /gene="tadA"
                     /locus_tag="PKI23_10465"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNN37399.1"
                     /translation="MTEPQSDDARWMALALELAAQAAAVGEVPVGAVVVREGQVIGTG
                     FNQVITTADPTAHAEVVALRMAARQLGNYRLSGSSLYVTIEPCSMCAGALVHARVGRL
                     IYGAREPRQGAVVSTLGVLDNPALNHRVHFQEGLMAEQAGALLTQFFRARRLRGRATT
                     LDQEMTE"
     gene            1356..5240
                     /gene="purL"
                     /locus_tag="PKI23_10470"
                     /gene_synonym="purI"
     CDS             1356..5240
                     /gene="purL"
                     /locus_tag="PKI23_10470"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNN37400.1"
                     /translation="MIELTTTEALSPFRLRRLVERLQQRQELVTGCATHHLYLVQSAR
                     PLDADESARLAALLEATPAVASSAAQELIVVPRFGTLSAWSSKATDIAHNCGLGAIQR
                     IERALVYRIDSRHRLEPQQWQQLLPLLHDRMTQQVLFDRDELAQLFSQAQAPTFTTID
                     LLGAGRAALVQANGALGLALAEEEIDYLVTAFQQLGRNPSDVELMMYAQANSEHCRHK
                     IFNAEWVIDGETQPLSLFGMIRNTHARHPAGVLSAYADNAAVIEGASGGRFFPDPHSH
                     RYQSHQEPIHILMKVETHNHPTAIAPHPGAATGAGGEIRDEGAVGLGAKPKAGLTGFT
                     VSNLRIPGFEQPWEVFHYGRPARIASPLEIMVDGPLGGAAFNNEFGRPNLLGYFRTFE
                     QQLETRYHGYHKPIMLAGGLGNIRAQHVQKQPYPAGTRLVVLGGPAMLIGLGGGAASS
                     VASGASDAELDFASVQRDNPEMQRRCQEVIDRCWQLGDENPIAFIHDVGAGGLSNALP
                     ELIKDGGRGGHFDLRAVPSAEPGLSPLEIWCNEAQERYVLAIPAERLERFVAICRRER
                     CPHAVVGEATEAQQLTLEDGQFGNRPVDLPLSLLFGNSPKMRRTITRQPHSLPPLRLE
                     GVKLDDAIERLLRLPSVASKQFLITIGDRSVTGLVARDQMVGPWQVPVSDLAVTAASF
                     DGYRGEAMAMGERTPLALIDAPASGRMAVGEAIINIAAARIDRLGDIKLSANWMAAAG
                     EAGEDEKLFDTVRAVGMELCPALGIAIPVGKDSLSMRTQWREAEADKKVVSPLSLIVS
                     AFAPVTDIRHTLTPQLDTGTPSELWLIDLGAGRNRLGASALAQVYGQVGSEGPDLDQS
                     ARLSAFFAAIQQLNRAGLLLAYHDRSDGGLFVTLAEMAFAARTGLRIELDRLIKREDE
                     LLPALFAEELGAVIQLRDSDREAVTRLLEQHQLLALTHPVASVASDGWLEIRHQGHCL
                     YRQRRSRLQQLWAETSHRIQALRDNSDCADEEFAAITDEQDPGLTVRPSFDSATNPAL
                     PYLNLGARPRVAILREQGVNGQVEMAAAFDRAGFDAIDLHMSQLIAGETTLADFKGLV
                     ACGGFSYGDVLGAGQGWARSILYNVRAREQFAAFFARVDSFSLGVCNGCQMMSGLKSL
                     IPGADHWPRFLRNRSEQFEARLVLVRVEASPSILLRDMAGSILPITVAHGEGRAVFSD
                     DGMQQASESAKTVALRYVDHDQRVTERYPFNPNGSPAGITALTSIDGRATIMMPHPER
                     LFRVSQHSWFPSRDGEAAPWLRMFQNARAWVA"
BASE COUNT          877 a         1737 c         1772 g          928 t
ORIGIN      
        1 ggccgatgca cagggcatgg tgacggtcaa cagcaccgcc ggcttgcagg cgctgcggat
       61 gcagaagccg gttcaggtgc tgggcagcgc ggtctacgac attgccgggc tgacccatca
      121 ggcaggattg gaccgcttct ggacggcgcc cggcgcgccc gatccgctgc tgctcgatgc
      181 cttcatcagg ctgctggtgc agcggaccca gcttcgaggg gtcttttttg cgccgtccgc
      241 gcaacatggc gcggtcatcg accgctttgc cgagcggctg ctgagcctgc ccccggcata
      301 gtgcgcgaca ttcagcccgg cggccagcgc atctgccgtc cggccagcag gtgcagatgg
      361 atgtggtaga cgctctgtcc cccctgctgg ttgcagttga acagcgtgcg aaagccggtg
      421 tcggcaaaac cgagccgctt ggccaactgg gtggcggtct ggatcatccg ccccatcagc
      481 agcgtgtcgt ccgcttccag atcattcagc gtggtgatgt gccggcgcgg aatcagcagg
      541 atgtgctgcg gcgcctgcgg attgatgtcg ttgaaggcga cgatctcgtc atcctcgtga
      601 acgagctggg tggagatttc gccgctggcg atcttgcaga acaggcagtt catggtgggg
      661 ttccgatgtc gagggtggtc tggcctgacg gggtgctgcc agtcactggc catgctgtga
      721 gaaaatggcg caaatcctat cacagtgccg tgaccgccag gcgtggcacg aaccggcgct
      781 gccgaccttc actccgagcg gactccatga ccttgcagca gtgctgtgat gactgcccat
      841 ttccattcag ccatgactga accgcagtcc gacgacgcac gctggatggc gctggcactc
      901 gaactggccg cgcaggccgc tgccgtcggc gaggtgccgg tgggtgcggt ggtggtgcgc
      961 gagggccagg tgatcggcac cggtttcaat caggtgatca ccacggccga tcccaccgcc
     1021 catgccgagg tggtggcgct gcggatggcg gcgcggcaac tgggcaacta ccgcctgtcc
     1081 ggcagcagcc tctatgtcac catcgagccc tgttcgatgt gtgccggagc actggtgcat
     1141 gcgcgggtgg ggcggttgat ctacggtgcg cgcgagccac ggcagggagc ggtggtctcg
     1201 accctcggcg tgctcgacaa ccctgcactc aaccaccggg ttcactttca ggaggggctg
     1261 atggccgaac aggccggtgc gctcctgacc caattctttc gtgcccgccg tctgcgtggc
     1321 cgagccacga ctcttgacca ggagatgacc gaatgatgat cgagttgacg accaccgagg
     1381 cgctttcgcc gttccgactg cgccggctcg tcgagcgttt gcagcaacga caggagctgg
     1441 tcaccggctg cgcgacccac cacctctatc tggtgcagag cgcccggccg ctcgatgccg
     1501 acgaatcggc acggctggcg gcactgctcg aggctacgcc ggcggtggcc tcctcggcgg
     1561 cgcaggagct gatcgtcgtg ccccgtttcg gcaccctgtc ggcctggtcg agcaaggcga
     1621 ccgacatcgc ccacaactgt ggcctcggcg ccatccagcg catcgagcgg gcactggtct
     1681 accgcatcga cagccgccat cgactcgaac cgcagcagtg gcagcaactg ctgccgttgc
     1741 tgcatgaccg catgacccag caggtgctgt tcgatcgcga tgaactggcg caattgttca
     1801 gccaagcgca ggcgcctacc ttcaccacca tcgacctgct gggggccggt cgcgctgcgc
     1861 tggtgcaggc caacggcgcc ctggggctgg cattggccga agaggagatc gactacctgg
     1921 tcacggcttt tcagcaactt ggacgcaatc cgagcgatgt cgagctgatg atgtatgccc
     1981 aggccaattc ggagcattgc cgtcacaaga ttttcaatgc cgaatgggtg atcgatggcg
     2041 agacgcagcc gctgtcgctg ttcggcatga tccgcaacac ccatgcacgg cacccggccg
     2101 gcgtgctcag cgcctatgcc gacaacgcgg cggtgatcga aggagcaagc ggcggacgct
     2161 tctttcccga tccgcacagc caccgttacc agagccatca ggagccgatt cacatcctga
     2221 tgaaggtcga gacccacaac cacccgaccg cgatcgcacc ccatccgggc gccgccaccg
     2281 gcgcgggcgg cgagattcgt gacgaagggg cggtggggct gggcgccaaa cccaaggccg
     2341 gcctgaccgg cttcacggtc tccaacctgc gcattcccgg cttcgaacag ccgtgggagg
     2401 tgttccacta cggccgaccg gcacgcatcg cctcgccgct ggagatcatg gtcgatggtc
     2461 cactgggtgg cgcggccttc aacaacgaat ttggccggcc caacctgctg ggctacttcc
     2521 gcaccttcga gcagcagctg gagacgcgat accacggcta ccacaagccg atcatgctgg
     2581 ccggtggact gggcaacatc cgtgcacagc atgtgcagaa gcagccctat ccggccggaa
     2641 cccggctggt ggtgctgggt ggcccggcga tgctgatcgg gctgggagga ggtgccgctt
     2701 catccgtggc ctcgggcgcc agcgatgccg agctcgattt cgcctcggtg cagcgcgaca
     2761 accccgagat gcaacgtcgc tgtcaggagg tgatcgaccg ctgctggcag ctgggcgacg
     2821 agaatccgat cgccttcatc catgacgtcg gcgccggcgg gctctccaat gccctgcccg
     2881 agctgatcaa ggatggcgga cgcggtggac acttcgatct gcgtgccgtg cccagcgccg
     2941 agccgggcct ctcgccgctg gagatctggt gcaacgaggc gcaggagcgc tatgtgctgg
     3001 cgataccggc cgagcggctc gagcggttcg tcgccatctg ccggcgcgag cgctgtcccc
     3061 atgccgtcgt cggcgaggcg accgaagcac agcagttgac gctggaagat ggtcagttcg
     3121 gcaaccggcc ggtcgatctg ccgctgtcac tgctgttcgg aaactcgccg aagatgcgcc
     3181 gcaccatcac ccgccagcca cattcgttgc caccgctgcg gttagagggc gtgaagctgg
     3241 atgacgccat cgagcgactg ctgcggctgc cgagcgtggc cagcaagcag tttctgatca
     3301 ccatcggtga tcgcagcgtc accgggctgg tggcgcgtga ccagatggtc ggcccctggc
     3361 aggtgccggt cagtgacctg gccgtcaccg ccgccagctt cgacggttac cgtggcgagg
     3421 cgatggcaat gggtgagcgc acgccgctgg cgctgatcga tgcgcccgcc tcgggccgca
     3481 tggcggtggg tgaggcaatc atcaacatcg ccgcggcacg catcgacagg ctcggcgaca
     3541 tcaaactctc ggccaactgg atggcggctg ccggcgaggc cggtgaggac gaaaagctgt
     3601 tcgacaccgt gcgtgcagtg gggatggagc tctgcccggc gctcggcatc gccatcccgg
     3661 tcggcaagga ttcgctgtcg atgcgcaccc agtggcgcga ggccgaagcc gataaaaagg
     3721 tggtctcgcc gctgtcgctg atcgtcagcg ccttcgcgcc ggtcaccgac atccgccaca
     3781 ccctgacccc acagctcgac accggcacgc cgagcgagct gtggctgatc gatctgggag
     3841 ccggtcgcaa ccggctcggg gcctccgcgc tggcgcaggt ctatggccag gtgggcagcg
     3901 aggggcccga tctcgatcag tcggcacggc tgagtgcctt ttttgcggcc atccagcagc
     3961 tcaatcgggc cggtctgctg ctcgcctacc atgaccgctc cgatggtgga ctcttcgtca
     4021 ccctggccga gatggccttt gccgcccgca ccggcctgcg gatcgagctc gatcggctga
     4081 tcaagcgcga ggatgagctg ttgccggcgc tctttgccga ggagctgggc gccgtcatcc
     4141 agctgcgcga cagtgatcgg gaggcggtca ccaggctgct cgaacagcac cagctgctgg
     4201 cgctgaccca tccggtggcc agcgttgcca gcgatggctg gctggagatc cggcaccagg
     4261 gccactgcct ctatcggcag cggcgcagtc ggctgcagca gctctgggcc gagaccagcc
     4321 accgcatcca ggcgctgcga gacaacagcg actgtgccga tgaggagttt gccgccatta
     4381 ccgatgagca ggatccaggt ctgacggtgc ggcccagctt cgacagcgcg accaacccgg
     4441 cgctgcccta tctcaatctg ggtgcccgcc cgcgggtggc gatcctgcgc gagcaggggg
     4501 tgaatggcca ggtcgagatg gcggccgcct tcgaccgggc cggcttcgat gccatcgatc
     4561 tgcacatgag ccagctcatc gctggcgaaa ccacgttggc cgacttcaag gggttggtgg
     4621 cctgcggtgg cttttcctat ggcgacgtgc tgggtgccgg tcagggctgg gccagaagca
     4681 tcctctacaa cgtgcgggcg cgggagcagt tcgccgcttt tttcgcgcgc gtcgacagct
     4741 tctcgctcgg tgtctgcaac ggctgtcaga tgatgtccgg cctcaagagt ctcattcccg
     4801 gtgccgacca ctggccgaga ttcctgcgca accgctccga acagttcgag gcgcggctgg
     4861 tactggtccg ggtcgaagcg tcgccctcca tcctgctgcg cgacatggcg ggctccatcc
     4921 tgccgatcac cgtggcgcat ggcgaggggc gggcggtgtt cagtgacgat ggcatgcagc
     4981 aggcgagcga atcggccaaa acggtggccc tgcgctacgt cgaccatgac cagcgcgtga
     5041 cggagcgcta tccattcaac cccaatggct cgccagcggg catcaccgcg ctcaccagca
     5101 tcgacggccg cgccaccatc atgatgccgc acccggagcg gctgttccgc gtctcgcagc
     5161 actcctggtt ccccagccgc gatggtgaag ccgccccttg gctgcggatg ttccagaacg
     5221 cgcgggcctg ggtcgcatga tttttcaatc agcgcttaaa aacctcttgc cacgactgga
     5281 ttggtccgta taatgcgctg ctctgacggt tgct
//
LOCUS       DAORCQ010000125         5296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_11501_length_5296_cov_5.574127, whole genome shotgun sequence.
ACCESSION   DAORCQ010000125 DAORCQ010000000
VERSION     DAORCQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11501_length_5296_cov_5.574127"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PKI23_10475"
     CDS             <1..1182
                     /locus_tag="PKI23_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNN37401.1"
                     /translation="KPKVAAYRAPGAPMAAFAMESMIDEMAHKLAIDPIQFRLDNAVR
                     EGSQAVYGPKFPAIGFEACLKQAESHPHYRAPLGANQGRGVAAGFWFNVGMQSSATVN
                     VQEDGSAIVITASPDIGGSRASMALMASEELGIPYERINALVGDTETAGYCDVTGGSR
                     TTFATGMAVIQASKDVIRQMRERAAKVWNVEIDQVSWVDGAAVPAAEVAAEKDLKPLS
                     FAELARNAAHTGGPIAGRASLMAQGAGAGFGVHLCDVEVDPETGKVDIIRYTVIQDAG
                     KAIHPAYVEGQLQGGAVQGIGWALNEEYVYNAQGVMENPAFLDYRMPVALDLPMIDAV
                     IVEVPNPSHPYGVRGVGETPIVPPLGAVANAVHDATQVRFCHLPLNPPRVLAGLQAAG
                     A"
     gene            1188..1433
                     /locus_tag="PKI23_10480"
     CDS             1188..1433
                     /locus_tag="PKI23_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37402.1"
                     /translation="MARVLLSSELQPLAGGVAELNLAAVDVRELFEQLRRHCPALAEH
                     VQQHSALAIDGVIYQRPFAEPLAPDCEIYFIPRIAAG"
     gene            1555..2217
                     /locus_tag="PKI23_10485"
     CDS             1555..2217
                     /locus_tag="PKI23_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNN37403.1"
                     /translation="MTFLLRHERSLLLLATLLVLLGSGWQPHDRATWWLEVAPVLIAL
                     PILLATHHRHPLTPLLYRLLFLHGVILMVGGHYTYARVPLGFWFADWFDLTRNHYDRL
                     GHLAQGFVPALLAREILLRATPLRGAGWIWLLVTAVALAVSALYELLEWAVALISAEA
                     AESFLAMQGDVWDTQWDIFLALIGALLAQWLCGRQHDRELARLRQRSITNPSPQSAPE
                     KH"
     gene            complement(2259..2648)
                     /gene="acpS"
                     /locus_tag="PKI23_10490"
     CDS             complement(2259..2648)
                     /gene="acpS"
                     /locus_tag="PKI23_10490"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNN37404.1"
                     /translation="MILGIGTDLVSVARIARLHQRYGDAFSRRILAPSELLELPTAAA
                     PAHFLAKRFAVKEAVVKALGCGFGNGVAPRQIALLHDSAGAPCLQLSGAAAERLAALD
                     AQAQVSLSDERDYALAFVVLSRAPLPV"
     gene            complement(2645..3385)
                     /gene="pdxJ"
                     /locus_tag="PKI23_10495"
     CDS             complement(2645..3385)
                     /gene="pdxJ"
                     /locus_tag="PKI23_10495"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNN37405.1"
                     /translation="MTSTDLLLLGVNIDHVATLRQARGTRYPDPVQAAFEAEEAGADG
                     ITLHLREDRRHIQERDVRLIQQLCQTRVNLEMAVTDEMVDFALDLRPAHVCLVPEKRQ
                     ELTTEGGLDLLAHEARITAAVGRMQAVGIEVSLFIDADPAQIDAAVRCRAKAIELHTG
                     HYAELPAGESSAELQRLQQAATQAHQLGLIVNAGHGLHYHNVEPIAAIAELNELNIGH
                     AIIARALFTGLKQAVRDIKALMRNARRP"
     gene            complement(3372..4169)
                     /gene="recO"
                     /locus_tag="PKI23_10500"
     CDS             complement(3372..4169)
                     /gene="recO"
                     /locus_tag="PKI23_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNN37406.1"
                     /translation="MTLGSTRPGDGTAEAAFVLHHRPYQEHGQLLDCLTREAGLLRLV
                     ARGSRGGSRNGRLLGALLQPFAPLRLAWSGRGELKSLTAAEASGMALTLRGERWWSGC
                     YLNELLLRLLSPFDPHPALFQAYGRTVTALAGDEAVAPLLRRFEWRLLTELGYAISFD
                     HTLDGALQIDVASSYRFDPAVGFIELARHEAASGHDCFSGAILLALAHDRLSESAALQ
                     AAKRLNELALAPHLRGRPLQTRALYRRARVALSMGADRDTQGLHDQH"
     gene            complement(4166..5077)
                     /gene="era"
                     /locus_tag="PKI23_10505"
     CDS             complement(4166..5077)
                     /gene="era"
                     /locus_tag="PKI23_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNN37407.1"
                     /translation="MSDHGTTASRCGLVAIVGRPNVGKSTLLNRILGQKLSITSRKPQ
                     TTRHRILGIKTNAAVQAIYIDTPGLQQKPTRAINRFMNREARSALLEVDLVLWVLEAG
                     LFTEEDESVLEGLRQSQVPVIAVLNKIDRLSDKGLLLPYLDTLSRRFAFAALLPVSAK
                     QGQQIELLEQTVAERLPLADHLYGDEELTDRSERFLAAELIREKIMRQLGEEIPYQVA
                     VEIERFVVENEIVHINALIYVEREGQKKILIGQGGERLKRIGQEARLDMERLFGNRVM
                     LTTWVKVKRGWSDDERALKSLGYQERE"
     gene            complement(5074..>5296)
                     /locus_tag="PKI23_10510"
     CDS             complement(5074..>5296)
                     /locus_tag="PKI23_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNN37408.1"
                     /translation="PKTRLQEHLQARHQPLPDYRVVRVEGKAHAQTFHVECHHPLLAE
                     PALGSGNSRRLAEQMAASTALKQLEAIET"
BASE COUNT          854 a         1725 c         1763 g          954 t
ORIGIN      
        1 aagccgaagg tggcggccta tcgggcaccg ggagcgccga tggcggcctt cgcgatggag
       61 tcgatgatcg acgagatggc gcacaagctg gcgatcgatc cgatccagtt ccgcctcgac
      121 aacgccgtgc gtgaggggtc gcaggcggtc tatggtccaa aattccccgc catcggcttt
      181 gaagcctgcc tcaagcaggc cgagagccat ccgcactacc gcgcaccact cggcgccaac
      241 cagggacgcg gcgtggcggc cggcttctgg ttcaacgtcg gcatgcagtc gagcgcgacg
      301 gtcaatgtgc aggaggatgg cagcgcgatc gtgatcaccg ccagccccga cattggcggt
      361 tcgcgcgcct cgatggcact gatggcctcc gaagagctgg gcattcctta cgagcgcatc
      421 aatgccctgg tcggcgacac cgagaccgcc ggctattgcg atgtcaccgg cggcagccgc
      481 accacctttg ccaccggcat ggcggtcatc caggcatcaa aagatgtgat ccggcagatg
      541 cgtgagcgcg cggccaaggt ctggaacgtc gagatcgatc aggtgagttg ggtcgatggt
      601 gcggcagtgc cggcggcaga ggtggctgcc gaaaaggatc tgaagccgct gagcttcgcc
      661 gagctggcca gaaatgcggc ccacaccggt ggtccgattg ccggacgcgc ctcactgatg
      721 gcacagggcg ccggcgccgg cttcggtgtg cacctctgcg atgtcgaggt cgatccggaa
      781 accggcaagg tcgacatcat ccgctacacg gtgattcagg atgccggaaa ggcgatccac
      841 cctgcctatg tcgaagggca gttgcagggc ggcgccgtgc agggcatcgg ctgggcattg
      901 aacgaggagt atgtctacaa cgcccagggg gtgatggaaa atcccgcctt cctcgactac
      961 cggatgccgg tcgccctcga cctgccgatg atcgatgcgg tgatcgtcga ggtgccgaat
     1021 ccgtcgcacc cctatggcgt gcgcggtgtc ggcgagacgc cgatcgtgcc gccgctgggt
     1081 gcggtggcca acgcggtgca tgacgccacc caggtgcgct tctgccacct gccactcaat
     1141 ccgccacggg tgctggcagg cttgcaggcc gccggagcct gaaccggttg gcccgggtgc
     1201 tgctgtcgag cgaactgcaa cccctcgccg gaggggttgc agagttgaat ctggctgccg
     1261 ttgacgtgcg cgaactgttc gagcagttgc gccgtcactg cccggcgctg gcggaacatg
     1321 tgcaacagca ttcggcgctc gccatcgatg gtgtcatcta ccagaggccc ttcgccgaac
     1381 cactggcgcc cgactgcgag atctacttca tcccccgcat tgccgctggc tgaatccggc
     1441 acatcggcca cccattcaga gccggcgaat ggctctgtcg gcggtcgaac tccctgctca
     1501 cggtcaggat cacggcattc agactgacca cttcagccgc acgtccgagt acccatgacc
     1561 tttctgctcc gacatgaacg cagcctgctg ctgctcgcca ccctgctggt gctgctgggc
     1621 tccggctggc aaccccatga ccgcgccacc tggtggctcg aggtggcacc ggtgctgatt
     1681 gcgctgccga tcctgcttgc cacccaccac cgccaccccc tgacaccgct gctgtatcgg
     1741 ttgctgtttc tgcatggggt gatcctgatg gtcggtggcc actacaccta tgcgcgagtg
     1801 ccactgggct tctggtttgc cgactggttc gatctgaccc gcaaccacta tgaccgcctc
     1861 ggccacctgg cgcagggttt tgttccggcg ttgctggcgc gggagattct gctgcgcgcc
     1921 accccgctgc gtggtgccgg ctggatctgg ctgctggtca cggcagtggc gttggccgtc
     1981 agcgcgctct atgaactgct ggagtgggcg gtggcgctga tcagcgccga ggcggcggag
     2041 tcctttctgg cgatgcaggg tgatgtctgg gacacccagt gggacatctt tctggcgctg
     2101 atcggcgccc tgctggcgca gtggctgtgt ggccggcagc atgaccgcga actggcgcgg
     2161 ctgcggcagc ggtcaatcac caatccgtca ccccagtctg cccctgaaaa gcactgaatt
     2221 tctgcgcgca gcatgaaagt acgccgatca cctcaccatc agaccgggag cggagcgcgg
     2281 ctcagcacca cgaaggccag cgcgtagtca cgttcgtcgc tcagcgagac ctgtgcctgg
     2341 gcatcgagcg ctgccagccg ctcggcggcg gcgccggaga gttgcaggca gggggcaccg
     2401 gcgctgtcat gcagcagcgc gatctgccgg ggtgccacgc cattgccgaa cccgcagccg
     2461 agcgccttga ccaccgcctc cttgaccgcg aaccgcttgg ccagaaagtg ggcgggtgcc
     2521 gcagcggttg gcagctccag cagttcgctg ggggcaagga tgcgccggga aaaggcatcg
     2581 ccatagcgct ggtgcagccg ggcgatgcgg gcgacgctga ccagatcggt gccgatgccg
     2641 aggatcatgg ccgccgtgcg ttgcgcatca gtgccttgat gtcgcgcacc gcctgcttga
     2701 gaccagtgaa gagcgcgcgg gcgatgatgg cgtggccgat gttgagttcg ttgagttcgg
     2761 caatggcggc gatcggctcg acgttgtggt agtgcaggcc atggccggca ttgacgatca
     2821 gtccgagctg atgggcttgg gtggccgcct gctgcagccg ttgcagttcg gcactgctct
     2881 cgccagcagg gagttcggca tagtggccgg tgtgcagttc gatggccttg gcgcgacagc
     2941 gcacggcagc gtcgatctgc gccggatcgg catcgatgaa cagcgacacc tcgatgccga
     3001 ccgcctgcat ccggccgacc gccgcagtga tgcgcgcttc gtgggcgagc aggtcgagcc
     3061 caccctcggt ggtcagctcc tgccgttttt ccggcaccag gcagacatgg gccggccgca
     3121 gatcgagcgc aaagtcgacc atctcgtcgg tcaccgccat ctccaggttg acccgggtct
     3181 ggcagagctg ctggatcagc cggacatccc gctcctggat gtgacggcgg tcttcgcgca
     3241 gatggagggt gatgccgtcg gcacccgcct cctcggcctc gaacgcggcc tgcaccggat
     3301 cgggatagcg ggtgccacgc gcctggcgca gggtggcgac atggtcgatg ttgacaccca
     3361 gaagcagaag gtcagtgctg gtcatggagg ccttgtgtgt ctcggtcggc acccatcgac
     3421 agcgccactc tggcacgtcg atagagcgcc cgggtttgca ggggacgccc gcgcaggtgc
     3481 ggcgccagcg ccagttcgtt cagccgtttt gccgcctgca gcgctgccga ctcgctcagt
     3541 cgatcatgtg ccagcgccaa caggatggca ccactgaagc agtcatggcc gctggctgcc
     3601 tcgtggcggg ccaactcgat gaaacccacg gccgggtcga accggtagct gctggccaca
     3661 tcgatttgca gtgcgccgtc cagggtgtgg tcgaaggaga tggcgtaacc cagttcggtc
     3721 agcagccgcc attcgaaccg gcgcagcagc ggcgccaccg cctcgtcacc agccagcgcg
     3781 gtcactgtgc ggccataggc ctggaacagc gccggatggg ggtcgaatgg actcaacagc
     3841 cgcaacagga gctcgttcag gtagcagccg ctccaccagc gttcgccgcg cagggtcagt
     3901 gccatgccgc tggcttcggc ggcggtcagg ctcttcagtt cgccgcgccc gctccaggcc
     3961 agccgcagcg gtgcgaaggg ttgcagcagc gcgcccagca gccgcccgtt tctgctgccg
     4021 ccacggctgc cacgggccac cagccgcagc agacccgctt cgcgcgtcag gcagtcgagc
     4081 agttggccat gctcctgata gggtcgatgg tgaagcacga acgccgcctc ggccgtgcca
     4141 tctccgggac gggtcgatcc cagtgtcatt cccgctcctg ataccccaga ctcttcaggg
     4201 cccgctcgtc atccgaccag ccccgtttga ccttgaccca ggtggtgagc atcacccggt
     4261 tgccgaacag ccgctccatg tcgagccgcg cctcctggcc aatgcgcttc aaccgttcgc
     4321 caccctggcc gatcagaatt ttcttctgcc cttcacgctc gacgtagatc agcgcattga
     4381 tgtggacgat ctcattctcg accacaaagc gctcgatctc gactgccacc tggtagggga
     4441 tttcctcgcc cagttgacgc atgatcttct cgcggatcag ttcggcggcc agaaaacgtt
     4501 cgctgcggtc ggtcagctct tcgtcgccgt agaggtggtc ggccagcggc agtcgctcgg
     4561 ccacggtctg ttccagcagc tcgatctgct gcccctgttt ggccgaaacc ggcagcagcg
     4621 cggcaaaggc aaagcgcctg ctcagcgtgt cgagataagg cagcagcagc cccttgtcgg
     4681 agagccggtc gatcttgttc agcacggcaa tgaccggcac ctggctctgc cggagccctt
     4741 cgagcacgct ctcatcctct tcggtgaaga gaccggcctc cagcacccag agcaccagat
     4801 cgacctcgag cagtgccgaa cgcgcctccc ggttcatgaa gcggttgatc gcacgggtcg
     4861 gcttctgttg caggcccggc gtgtcgatgt agatggcctg caccgctgcg ttggtcttga
     4921 tgccgagaat gcggtgacgc gtggtctgcg gcttgcgtga ggtgatgctg agcttctggc
     4981 cgaggatgcg gttgagcagg gtcgacttgc cgacattggg ccggccgacg atggcgacca
     5041 gaccgcagcg gctggcagtc gtgccgtggt cactcatgtt tcgatggcct ccagttgctt
     5101 cagggcagtg ctggccgcca tctgctcggc cagccggcgg ctgttgccgc tgccaagcgc
     5161 cggttctgcc agcaacggat ggtggcactc gacatggaag gtctgggcgt gcgccttgcc
     5221 ttcgacccgc accacccggt agtcgggcag tggctggtgc cgggcctgca gatgctcctg
     5281 caggcgggtc ttgggg
//
LOCUS       DAORCQ010000126         5265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_11582_length_5265_cov_5.048752, whole genome shotgun sequence.
ACCESSION   DAORCQ010000126 DAORCQ010000000
VERSION     DAORCQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11582_length_5265_cov_5.048752"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKI23_10515"
     CDS             <1..706
                     /locus_tag="PKI23_10515"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNN37409.1"
                     /translation="AIAVAMAEQGARVVISSRKAEACQQVAQQINQTHQAPDRAIALP
                     CNIADKRQLQQLVDETRRQLGQIDILVCNAAVNPYFGPSATIPDDAFDKVLHSNVKSN
                     HWLCQMVAPEMVERRDGVIIVVSSVGGLHGSAVLGTYALSKAADMQLVRNLAVEYGAH
                     NLRANTIAPGLIRTDFSRALWENPEVLKRSTEQTPLRRIGEPEEIAGAAVFLASAAGR
                     FMTGQTLVIDGGGSIA"
     gene            782..3913
                     /locus_tag="PKI23_10520"
     CDS             782..3913
                     /locus_tag="PKI23_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNN37410.1"
                     /translation="MDSSPSSEPLNLLLVGRELDDLARIREVLAETGIASRLEITTDS
                     QRALEWLCTTRQGVCLLNSRLQGMRGIDLLQAAREAGCHLPIIMLTCSSDVEDEQAAR
                     QAGAFDCLNKNTLLPALLGCSLRHAVNEADRIRQLKQQQQTVRQLYQQLELVRGVLDQ
                     HAIVSEADRAGNIIAVNDLFCTISGYSREELLGQNHRLLKSGEHPAELYQQLWSTISR
                     GQSWHGEICNRRKDGELYWVQATIVPIADEHGRPQRYISIRTDITAQKRDELALRLLG
                     HAVRASASGIAIMDASQANPRIRYVNPALNRMTGYSPDELVAGGSALLFGPQTDMQSR
                     AEALQQLRAGQSVRQLLLCLYRKNGTPFWCDFSASWALDEQGDKTSLYVVVLNDVTEQ
                     KMAEDALRESEERLRRSQNYANIGTWDLNIQTGELLWSEQIAPMFGYQGVVETSYENF
                     IDAVHPEDRQSVIDAISGCIERGEKYRIQHRVQWPDGTLRWLLEQSDVTRLADGTPTH
                     MLGVVQDVTVHKEVSEQLRINQARLTNAQRIAHLGNWDLDLASNRLHCSEQIYAILDI
                     DPAVQIDLDRFYASVHPDDLAQIKTFRTQVLHDGKTEQSYRILRPDGEVRHLRAITEA
                     SFAADGRPLTLSGTTQDITEQVLREAALRQAKQEAEQANQAKSEFLSSMSHELRTPMN
                     AILGFAQLLQLRRELTTEQQQDVAEILKAGRHLLELINEVLDLSRIEAGALSLSLEPV
                     LCCEAIDESLALLQPMAEHHGILLREDPRKVCFVWFQADRTRLKQVLVNLISNAIKYN
                     RPHGSVTLRMVSGSPGFARIEVSDTGYGIDEALLPLLFQPFQRLDAATRGIEGTGIGL
                     AICKRLVESMGGTLGVESSPDIGSTFWLELPRIEPATPAAAHLTEMASPALPASMAAA
                     TLLYVEDNPANLRLVEQIVATRPHWRLLSASTPEIGLELIEVHANTLDVILLDIDLPG
                     MNGYQMLKRLQDEPRTAAIPVLALSANAMPNDIRRGLAAGFLDYLVKPIEVPEFLRIL
                     DGLLEQRPTQPKT"
     gene            3920..5008
                     /locus_tag="PKI23_10525"
     CDS             3920..5008
                     /locus_tag="PKI23_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN37411.1"
                     /translation="MPPLTDTEIKNAAIAIVDDEPANVLLVERTLSGAGYRTLHSTTD
                     SRQALELIRQHHPALLLLDLNMPHLNGLQLMAQMAATLSDPPTVVVLTAQTALEHRLN
                     VLQQGARDFISKPFEIPELLARVHNSLETHLLQARLRNDNILLEQRVAERTQELHDSR
                     LEIIRRLGRAAEFRDNETGLHIIRMSKYSELLGRCAGMSDGEAELLLQASPMHDIGKI
                     GIPDQILLKPGKLNAEEWETMKQHAQIGADLLDGSSAPLLVVAREIALTHHEKWDGSG
                     YPHALRGESIPLVGRIVAVADVFDALTSERPYKAAWPIPKAIELLQAERDRHFDRRLV
                     DLFIANLDAVLAIRERHAEPHSGSEATH"
     gene            complement(5043..>5265)
                     /locus_tag="PKI23_10530"
     CDS             complement(5043..>5265)
                     /locus_tag="PKI23_10530"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HNN37412.1"
                     /translation="FDQTQYLDCARHWCERLAAEHGLALQWNGEVEQAALLWSRSRGH
                     RSGRAAEQFARDQVAGRLVAARLSLHKSG"
BASE COUNT         1036 a         1779 c         1599 g          851 t
ORIGIN      
        1 ggcgattgcc gtggcgatgg cggagcaggg cgcacgggtg gtgatctcca gccgcaaggc
       61 cgaggcctgc cagcaggtgg cgcagcagat caatcagacc catcaagccc ccgaccgggc
      121 cattgccctg ccctgcaaca tcgccgacaa gcggcagttg cagcagttgg tcgatgagac
      181 ccgacggcag ttaggccaga tcgacatcct ggtctgcaat gctgccgtca acccctactt
      241 cggcccctct gccaccatcc cggacgacgc cttcgacaag gtgctgcaca gcaacgtcaa
      301 gtcgaaccac tggctgtgcc agatggtggc gccggaaatg gtcgaacggc gcgacggggt
      361 gatcatcgtc gtctcgtcgg tgggcggact gcatggctcg gcagtgctcg gcacctatgc
      421 gctctccaag gccgccgaca tgcagctggt gcgcaacctg gcggtcgaat atggcgccca
      481 caacctgcgc gccaacacca tcgcgcccgg tctgattcga accgacttct cccgcgctct
      541 gtgggagaac cccgaggtgc tgaagcgatc gaccgaacag acgccgctgc ggcgcatcgg
      601 tgaacccgaa gagatcgccg gcgccgcggt ctttctcgcc tcggccgccg gtcgcttcat
      661 gaccggtcag accctcgtca tcgatggcgg tggcagcatc gcctgaacag gctatatttt
      721 caccatccgc tcggcgtgat ataacaacaa taaaaacagg gataaaggaa tggttcctcc
      781 ggtggactct tcgcccagca gcgaaccgct gaatctgttg ctggtgggca gggagctcga
      841 cgacctggcc cgcatccgcg aggtgctggc cgagaccggc attgccagcc ggctggagat
      901 caccaccgac agccagcggg cactggagtg gctctgcacc acccgccaag gtgtctgcct
      961 gctgaacagc cggctgcaag ggatgcgcgg catcgacctg ctgcaggccg cgcgcgaggc
     1021 cggctgccac ctgccgatca tcatgctgac ctgcagcagc gatgtcgaag atgaacaggc
     1081 cgcccgccag gcgggcgcct tcgactgcct gaacaagaac acactgcttc ccgccctgct
     1141 cggttgcagc ctgcgtcatg ccgtcaatga agccgatcgc atccgccaac tcaaacaaca
     1201 acagcagacc gtgcggcagc tttaccagca actggagctg gtcaggggcg tgctggacca
     1261 gcatgccatc gtcagcgagg ccgaccgcgc cggcaacatc atcgcggtca acgatctgtt
     1321 ctgcaccatc agcggctaca gccgcgagga gctgctgggg cagaaccacc gcctgctcaa
     1381 gtcaggggaa catcccgctg aactctatca gcagctctgg tcgacgatca gccgcggcca
     1441 gagctggcat ggcgagatct gcaaccggcg caaggatggc gaactttact gggtgcaggc
     1501 caccatcgtc cccattgccg acgagcatgg ccggccccag cgctacatct cgattcgcac
     1561 cgacatcacc gcacagaagc gtgacgaact ggcactgcgt ctgctgggcc atgcggtgcg
     1621 tgccagcgcc tccggcatcg ccatcatgga cgccagtcag gccaatccgc gaattcgcta
     1681 cgtcaacccg gccctgaatc gcatgaccgg ttacagcccc gatgagctgg tggccggcgg
     1741 cagcgccctg ctgttcgggc cacagaccga catgcagagc agagcagagg cgctgcagca
     1801 actgcgcgcc ggccagtcag tccggcagtt gctgctttgc ctctaccgaa agaacggtac
     1861 gcccttctgg tgtgatttct ctgcttcctg ggctctcgat gagcagggcg ataaaacgtc
     1921 cctttacgtc gtcgtgctca acgatgtgac cgagcagaag atggccgagg atgcgctgcg
     1981 tgaaagcgaa gagcggctgc gccgcagcca gaactacgcc aacatcggca cctgggactt
     2041 gaacattcaa accggagaac tgctgtggtc cgaacagatc gcaccgatgt tcggttacca
     2101 aggggtggtc gagaccagct atgagaactt catcgatgcc gtccatcccg aagatcggca
     2161 aagcgtcatc gatgcgatca gtggctgcat cgagcggggc gagaagtatc ggatccagca
     2221 ccgtgtgcag tggcccgatg gcacccttcg ctggctgctg gagcagagcg atgtcacccg
     2281 gctggccgat ggcacaccga cccacatgct cggcgtggtg caggatgtca cggtccacaa
     2341 ggaggtctcg gagcagttgc gcatcaacca ggcccgcctg accaatgccc agcgcatcgc
     2401 ccatctcggc aactgggatc tcgatctggc cagcaaccgc ctgcactgct ccgagcagat
     2461 ctatgccatc cttgacattg atcctgccgt gcagatcgat ctggatcgct tctatgccag
     2521 tgtgcacccc gacgacctcg cccagatcaa gacattcaga acccaggtgc tgcacgatgg
     2581 caagaccgaa cagagctacc gcatcctgcg ccccgatggc gaggttcgcc acctgcgcgc
     2641 gatcaccgag gccagcttcg cggccgatgg ccggccgctg acactgagcg gcacgacgca
     2701 ggacatcacc gaacaggtgc tgcgcgaggc ggcgctgcgc caggccaagc aggaggccga
     2761 gcaggccaac caggccaagt cggagtttct ctccagcatg agccatgagc tgcgcacgcc
     2821 gatgaacgcg attctgggct ttgcccagct tttgcaactg cggcgcgaac tgaccactga
     2881 acagcagcag gatgtcgccg aaatcctcaa ggccggccgc cacctgctgg agctgatcaa
     2941 cgaggtgctc gacctctcca ggatcgaggc cggagcactg tcgctgtcgc tggagccggt
     3001 gctctgctgt gaggcgatcg atgagtcact cgcactgctg cagccgatgg ccgaacacca
     3061 tggcatcctg ctgcgcgagg atccgcgcaa ggtctgtttc gtctggttcc aggccgaccg
     3121 cacccgcctg aagcaggtgc tggtcaacct gatctccaat gcgatcaagt acaaccgccc
     3181 gcatggcagc gtcacgctgc gcatggtcag tggctcgccc ggctttgccc gcatcgaggt
     3241 gagcgacacc ggctacggca tcgatgaggc gcttctgcca ctgctgttcc agccgttcca
     3301 gcggctcgat gccgccaccc ggggcatcga aggcactggc atcggcctgg cgatctgcaa
     3361 gcggctggtc gaatcgatgg gcggcacact cggcgtggag agcagccccg acatcggcag
     3421 caccttctgg ctggagctgc cacggatcga accagcaacg cccgcggcag cgcatctgac
     3481 cgaaatggcc tcccccgcac tgccagccag catggccgcc gccacgctgc tctatgtcga
     3541 agacaacccg gccaacctgc ggctggtcga gcagatcgtc gccacccgac cccactggcg
     3601 gctgctcagc gccagcaccc cggagatcgg actggaattg atcgaagttc atgccaatac
     3661 tctggatgtg attctgctcg acatcgacct gcccggcatg aacggttacc agatgctgaa
     3721 acgactccag gacgaacccc gcaccgcagc cattccggtg ctggcactga gcgccaacgc
     3781 gatgccgaat gacattcgcc gcggcctggc cgccggtttt ctggactatc tggtcaaacc
     3841 gatcgaagtg ccggagttcc tgcggattct cgatgggttg ctcgagcagc gccccacaca
     3901 acccaaaacg tgaatcccga tgccaccgtt gaccgatacc gagatcaaga acgccgccat
     3961 cgccatcgtc gatgacgagc cggccaatgt gctgctggtc gaaaggacgc tcagcggtgc
     4021 cggctaccgc actctgcact cgaccaccga ttcacggcag gcgctggagt tgatcagaca
     4081 gcaccacccc gcgctgctgc tgctcgacct caacatgccc catctgaatg ggctgcaact
     4141 gatggcgcag atggcggcaa ccctctccga cccgccgacc gtggtggtgc tgacggcgca
     4201 gaccgcgctg gagcatcggc tcaacgtgct gcaacagggt gcccgcgact tcatttccaa
     4261 gccgttcgag attcctgagc tgctggcacg ggtgcacaac tcgctggaga cccatctgct
     4321 gcaagcgcgt ctgcgcaacg acaacatcct gctggagcag cgggtcgccg agcgcaccca
     4381 ggagctgcat gacagccgcc tggagatcat ccgccggctg gggcgcgccg ccgagttccg
     4441 cgacaacgag accggcctgc acatcatccg catgagcaaa tactccgaac tgctggggcg
     4501 ctgtgccggc atgagcgacg gcgaggccga actgctgctg caggcgagcc cgatgcacga
     4561 catcggcaag atcggcattc ccgaccagat tctgctcaaa ccgggcaagc tgaacgccga
     4621 ggagtgggag accatgaaac agcacgccca gatcggcgcc gacctgctcg atggcagcag
     4681 tgcgccactg ctggtggtgg cacgcgagat tgccctgacc caccatgaaa agtgggatgg
     4741 cagcggctac ccccatgcgc tgcgcggcga gtcgatcccg ctggtggggc gcatcgtcgc
     4801 ggtggccgat gtgttcgacg cgctgaccag cgagcgcccc tacaaggcgg cctggccgat
     4861 ccccaaggcg atcgaactgc tgcaggccga gcgcgaccgc cacttcgatc gccggctggt
     4921 cgatctgttc attgccaacc tcgatgccgt gctggcgatc cgcgaacgcc acgccgaacc
     4981 gcactccggc tctgaagcca cgcattgagc gccagtgccg gagcccatcc cggcgccttg
     5041 catcagccgg atttgtgcag gctcaatcgc gccgcaacga gccgaccggc cacctgatcg
     5101 cgcgcaaact gctcggcggc acggccactg cgatgacccc ggctgcgcga ccagagcagt
     5161 gccgcctgtt cgacctcacc gttccactgc aatgccagcc catgctcggc cgccagccgc
     5221 tcgcaccagt ggcgggcaca gtcgagatac tgcgtctgat cgaag
//
LOCUS       DAORCQ010000127         5255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_11618_length_5255_cov_5.185577, whole genome shotgun sequence.
ACCESSION   DAORCQ010000127 DAORCQ010000000
VERSION     DAORCQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11618_length_5255_cov_5.185577"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..2571)
                     /locus_tag="PKI23_10535"
     CDS             complement(271..2571)
                     /locus_tag="PKI23_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37413.1"
                     /translation="MAHHRSSRNSARSLLMLLGILLSHGLFAQVSSDTVSSFTSLPPV
                     TTPSTPPLLLIAMSRDHELFRKAYSDYSDIDGDGQIDTTYKDSIEYLGYFDSNFCYQY
                     DNGKFSPIGQVNSGTHKCSTVDGWSGNFLNWASMTRIDLIRTVLYGGKRQVDPTSSSG
                     NVLLERELIPPDIHAFAKVYDGAASGAIALADLVQGSPANGAITLCNVSKWPASTAQS
                     GTLDTSSYPPLLRVAYGNATGRVGYPNWGATEVVQCQWNGTANSAGDADRPAGNDTSN
                     AYKVLVERCVASQDGPVSARCKRYDNNTPSNSSDDLFKPIGLLQRYGERGQMRFGLMT
                     GSYDNNSHGGVLRQGVGLLSEVSTTTGKITADKLIGTLDKLRIATYRRASASALGSGG
                     GYQNCGDYVPASYVKNGSCADWGNPLGEIYLEAVRYLAGASAASSAYLVTEGPTATEN
                     DMVPGLTRLTSWSDPYNFGPSSSAVPCAQCALLLISTSQGSFDSMRDAADWPSSGLPT
                     GFAVATATNAIQTKEGLDGVSVLAGRVGSSTDQECSPKTMAAGTLDQVDGICPEAPSA
                     EGGYDIAGLAYEAAVKDLRTGLSGTQKIKTFAVSVAEDLPSFIVTSSTNAAKQAQIVP
                     LCKSRYSHNVAVSHDFQKCSLVDVIKESNPSTLPSPCTSNGSYHRFLLTWEGTAWGSD
                     HDFDMEQRLEYCEAAGNLWVRTAVTRDQAVAAPRAAMRSRMLRMRSRSRSPSPPGRTK
                     PITYATVPAASAARVPRCPPRDWSTA"
     gene            complement(2599..3216)
                     /locus_tag="PKI23_10540"
     CDS             complement(2599..3216)
                     /locus_tag="PKI23_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNN37414.1"
                     /translation="MAIHRGGNRQRGAILVVGLIVLLLMTLLGLSIMGGNAVEEKMAG
                     NERDRQIAFQAAEAALREGERLIVQTNSWQTRVNDNCTNGFCKSKKLDANYAASIGVS
                     SCSANSLEERWVDIPGCTGSRNVWSTANRHRSYTTSAFSEVNAQAMYIIELVGNSIEP
                     PNDITTCPSVMVSDICPDVFRVTAVGIGGTSATRVVLQSTYKKPL"
     gene            complement(3254..4318)
                     /locus_tag="PKI23_10545"
     CDS             complement(3254..4318)
                     /locus_tag="PKI23_10545"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNN37415.1"
                     /translation="MVAMVIGLLLLSGLVNVLVGSKKSYQWQDELSRLQENGRFAVQT
                     LSREIRLAGYAGCSPVINNLLNPAGTGYSNSLFDLSEVATGWEFTGTAVGNSYAMNST
                     NPTGVALSSWDDPALADLAAGLQNKVVPGTDVLVLKRASAKAGVTASGNTPANANTVN
                     LTAASGIAAGTILMISDCSGADVFQNRANANAHNVTRGASNSNPGPGNVNPGHYNFSH
                     RYESDAEIYTVAVTVFYIGISAGGEPALYQISYDMGSAGTPVALVEGVENMQLLYGID
                     LNGDDVPEQYVTAAALGGNADRVRTIRLGLLLRSFAKVDKDSVISSYDLLGTTLTTPN
                     DGRNRSVLTTTVKLRNRGQM"
     gene            complement(4384..4860)
                     /gene="pilV"
                     /locus_tag="PKI23_10550"
     CDS             complement(4384..4860)
                     /gene="pilV"
                     /locus_tag="PKI23_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNN37416.1"
                     /translation="MKKGASHAKEAGFSLIEGLVALVIFSIGLLGMAGLHSTGMRINS
                     ESWQRARANNMAMDILDRIRANRDEALANNIYLTDLTDASTTYAACSGACGSAQAVAK
                     NDLRQWKSTIESQLPAGKAGIAVVNPGPNPVYVVTVQWDESRGTGTPISYVIRSEL"
     gene            complement(4875..>5255)
                     /locus_tag="PKI23_10555"
     CDS             complement(4875..>5255)
                     /locus_tag="PKI23_10555"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HNN37417.1"
                     /translation="KRGKTVTLCASSDQQSCSTAWSSGWLLFVDNNSNSALNNGEEIL
                     RVGRAVNGRNSLAMSGSVSNNAIQFKSSGFSNQNATWTYCAADKVVSKARGIVVESSG
                     LTRRSRDTNGDGVQEGFSAALSCP"
BASE COUNT          898 a         1647 c         1656 g         1054 t
ORIGIN      
        1 cccgaacggg ggtgagcggc gcagcgtgcg gaacacgatc ctggggctga cggtgctctc
       61 ggtggtcgag gtgaagcgcg aggggctgga tcaggccgac gagtatggca gcgtggtgac
      121 gacggtcttc ctggtgctcg gcatcttctc ctccacgtgc gtgatgcacg ggtcggtgcg
      181 gcggatgctc agcacgccgc tgtcggagct gtggccgccg cggcagatgt tctccatcgc
      241 cgttgggtac gcggccgggt tcctcaccgc ctacgccgtc gaccagtccc tgggcgggca
      301 cctggggacc ctcgcggcgc tggccgcggg caccgtcgcg tacgtgatcg gcttcgtcct
      361 gcccggcggg ctcggggagc gcgaccggga ccgcatccgc agcatccggg agcgcatcgc
      421 cgcgcgcggg gcggccaccg cctgatcccg ggtcaccgcc gttctgaccc agaggttccc
      481 ggccgcctcg caatactcca gccgctgctc catgtcgaag tcatggtcac tgccccaggc
      541 ggtgccctcc caggtcagca gaaagcggtg gtagctgcca ttgctggtgc aaggggatgg
      601 gagtgtggac gggttcgact ccttgatcac atcgaccagc gagcatttct gaaagtcatg
      661 cgagactgcc acattgtggg agtacctcga cttgcacagc ggcacgatct gcgcctgctt
      721 ggcagcgttg gtgctgctgg tgacgatgaa gctgggcagg tcttcggcca ccgagactgc
      781 aaaggtcttg atcttctggg tgccgctcaa accagtacgc agatccttga cggccgcctc
      841 gtaggccagt ccggcgatgt catagccacc ctcggcactg ggcgcctcgg ggcagatgcc
      901 atccacctga tcgagcgtgc ccgccgccat ggttttggga ctgcactcct gatcggtgct
      961 gctgcccaca cggccagcca gcacgctgac gccatccagc ccctccttgg tctgtatcgc
     1021 attggtggcc gtggccacgg caaatcctgt cggcaagccg ctgctgggcc agtcggcggc
     1081 atcgcgcatc gagtcgaacg agccctgtga ggtggagatc agcaacagcg cgcactgggc
     1141 gcaaggcacg gcactgctgc tggggccgaa gttgtagggg tcgctccaac tggtcaggcg
     1201 ggtcaaccct ggaaccatgt cgttttcagt ggctgtcgga ccctcggtga ccagataggc
     1261 cgacgaggcc gcactggccc cggccagata gcgcaccgct tcgaggtaga tctcgccgag
     1321 cgggttgccc cagtcggcgc aggagccatt cttgacatag ctggcaggca catagtcgcc
     1381 gcagttctgg tagccaccac cgctgccgag cgcgcttgcg ctggcccggc ggtaagtcgc
     1441 gatgcgcagc ttgtcgagcg tgccgatcag tttgtcagcg gtgatcttgc cggtggtggt
     1501 gctgacctca ctgagcagac ccaccccctg gcgcagcacg ccgccgtggc tgttgttgtc
     1561 gtaactgccg gtcatcaggc caaaacgcat ctggccacgc tcgccatagc gttgcagcag
     1621 accgatcggc ttgaacaggt catcgctgct gttgctcggc gtgttgttgt cgtaacgctt
     1681 gcagcgggca gagaccggac catcctgact ggccacacag cgctcgacca gcaccttgta
     1741 ggcattgctg gtgtcattgc cagcagggcg gtcggcatca cccgccgagt tggcagtgcc
     1801 attccactga cactgcacca cctcggtcgc cccccagttg gggtagccga cccgtcctgt
     1861 ggcattgcca taggccaccc gcagcagagg agggtagctg ctggtgtcga gtgtgccgct
     1921 ctgagcggtc gaagccggcc acttggagac attgcacagc gtgatggcgc cattggccgg
     1981 actgccctgc accagatcgg ccagcgcgat ggcgccgctg gcagccccat catagacctt
     2041 ggcaaaggca tggatgtcgg gcgggatcag ttcgcgctcc agcagcacat tgccactgct
     2101 gctggtggga tcgacctgcc gtttgccgcc atagagcacg gtgcggatca ggtcgatgcg
     2161 ggtcatgctg gcccagttga gaaagttgcc actccagcca tcgacagtgc tgcatttgtg
     2221 agtcccactg ttgacctgcc cgatcggaga aaacttgccg ttgtcgtact gataacagaa
     2281 gttgctgtcg aaatagccga gatactcgat gctgtccttg taggtggtgt caatctggcc
     2341 atcgccatcg atgtcgctgt agtcggagta ggccttgcgg aacagctcgt gatcgcgcga
     2401 catcgcaatc agcagcagcg gtggcgtgct gggggtggtg accggtggca atgaagtgaa
     2461 actgctcacg gtgtcggatg agacctgcgc gaacagccca tggctgagca ggatgcccag
     2521 cagcatcagc agggagcgcg cgctgtttct gctggagcgg tgatgtgcca tggcggattc
     2581 ctcttgcgtc aacatctact acaagggttt tttataggtg ctctgcaaca cgacccgggt
     2641 tgcagaggtg ccaccgatgc cgaccgcagt gacgcgaaag acatccgggc agatgtcgga
     2701 caccatcacg ctggggcagg tggtgatgtc gttgggcggt tcgatggagt tgccgaccag
     2761 ttcgatgatg tacatcgcct gggcattgac ttcactgaag gccgaggtgg tgtagctgcg
     2821 gtggcgattg gccgtgctcc agacattgcg gctgccggtg cagcccggaa tgtcgaccca
     2881 gcgctcctcc aggctgttgg cgctgcaact gctgacacca atgctggccg catagttggc
     2941 atccagcttc ttcgacttgc agaagccgtt ggtgcagttg tcattgaccc gcgtctgcca
     3001 actgttggtc tgcacgatca agcgctcacc ttcgcgcaag gcggcctcgg ccgcctgaaa
     3061 ggcgatctgc cgatcacgct cgttgccggc catcttctct tcgacggcat tgccgcccat
     3121 gatcgacagt cccagcagtg tcatcagcag caatacgatc agtcccacga ccagtatcgc
     3181 gccccgctgc cgattgccgc ctcgatgtat cgccatgccg ttcgatcgct tcatctttgg
     3241 ttcgcccctg aaatcacatc tgcccgcggt tgcgcagctt gaccgtcgtg gtcagtaccg
     3301 agcggttgcg cccatcgttg ggggtggtga gggtggtgcc caacaggtca taactggaaa
     3361 tcacgctgtc tttatcgacc ttggcgaagg agcgcagcag cagtccgagc cggatggtgc
     3421 gcacccggtc ggcattgccg cccagcgccg ctgcggtcac atactgttcc ggcacatcgt
     3481 cgccattcag atcgatgcca tagagcagtt gcatgttctc gaccccctcc accagcgcca
     3541 ccggcgtgcc ggcactgccc atgtcatagg agatctggta gagcgccggc tcaccgccag
     3601 cgctgatgcc gatgtagaaa accgtcaccg ccaccgtgta gatttcggca tcggactcat
     3661 agcggtggct gaaattgtag tggccggggt tgacgttgcc tgggcccgga ttgctgttgc
     3721 tggcgccgcg ggtgacgttg tgggcattgg cgttcgcccg attctggaac acatcggcgc
     3781 cactgcaatc ggagatcatc aggatggtgc ccgctgcaat gccgctggca gccgtcaggt
     3841 tgacggtgtt ggcgttggcc ggggtgttgc ccgatgcggt gacgccggcc ttggccgaag
     3901 cgcgcttcag caccagcaca tcggtgcccg gcaccacctt gttctgcagg ccagcggcaa
     3961 gatcagccaa ggccggatca tcccaggatg acaatgcaac ccctgtcgga ttggtgctgt
     4021 tcattgcata gctgttgccc acggcagtgc cggtaaactc ccatccggta gccacctcac
     4081 tcagatcaaa cagagaattg ctgtagccag tgcccgccgg attcagcagg ttgttgatga
     4141 cgggtgagca gcccgcataa ccggccaggc gaatctcccg gctcagcgtc tgcacggcaa
     4201 accggccatt ctcctgcaga cgcgacagct catcctgcca ctggtagctc tttttgctgc
     4261 cgaccagcac attgaccagc ccgctcaaca gcagcaggcc gatcaccatt gctaccatca
     4321 attcgatcag ggtaaccccg gactgcctgc ttgaatgggt gtgccttttc atcgttgccg
     4381 gcctcacagt tctgagcgga tcacgtaact gatcggcgtg ccggtgccgc ggctctcatc
     4441 ccactgcacg gtgacgacat agaccggatt ggggcccgga ttgaccacgg caatgccagc
     4501 cttgccggcc ggcagttgcg actcgatggt cgacttccac tgccgcaggt cattcttggc
     4561 caccgcctgg gccgagccgc atgcgccgct gcaggcggca taggtcgtgg aggcatcggt
     4621 cagatcagtc aggtagatgt tgttcgccaa cgcttcatcc cggttggccc ggatgcggtc
     4681 gaggatgtcc atcgccatgt tgttggcgcg ggcacgctgc cagctctcgg agttgatgcg
     4741 catgccggtc gagtgcagcc cggccatgcc gagcagaccg atcgagaaga tcaccagcgc
     4801 caccagcccc tcgatcaggc tgaagcctgc ctcttttgcg tgtgatgcac cttttttcat
     4861 ctgccaccgc cccctcatgg acatgacaac gctgctgaaa aaccctcttg cacaccgtca
     4921 ccgttggtgt ctcgcgatct gcgggtcagg cccgaacttt cgacgacgat gccccgggcc
     4981 ttgctgacca ccttgtcggc ggcacagtag gtccaagtcg cgttctgatt ggagaagcca
     5041 ctgctcttga actggatggc gttgttcgag accgatccgc tcatcgccag actgttgcgg
     5101 ccattcacgg cccggccgac gcgcaggatc tcctcgccat tgttgagcgc gctgttggag
     5161 ttgttgtcga cgaacagcaa ccagccactg ctccaggccg tgctgcaact ctgctggtca
     5221 ctgctggcac agagggtgac cgtcttgccg cgctt
//
LOCUS       DAORCQ010000128         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_11697_length_5234_cov_5.325932, whole genome shotgun sequence.
ACCESSION   DAORCQ010000128 DAORCQ010000000
VERSION     DAORCQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11697_length_5234_cov_5.325932"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /gene="leuC"
                     /locus_tag="PKI23_10560"
     CDS             complement(<1..1169)
                     /gene="leuC"
                     /locus_tag="PKI23_10560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNN37418.1"
                     /translation="MGGATLYDKLWNDHVVVERDDGSALIYIDRHIVHEVTSAQAFDG
                     LRLAGRKPWRADSVLATPDHNVPTTERSSGVAGIVDPISRLQVQTLDDNCREFGLTEF
                     RMNDPRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFAALAQGIGTSEVEHVLASQ
                     CLVQKKLKNMRIEVSGTLAPGVTAKDLVLHIIGAIGTAGGTGYAIEFSGSAIRALSME
                     GRMTLCNMAIEAGARTGLVAVDQTTLDYLQGRPYAPKGELWQQAVAHWRTLISDADAR
                     FDACFEFDAAAVKPQVSWGVSPEMVLPIDAAIPDPAAQSDAVRREGYGRALEYMGLRA
                     GQPISEIAVDRVFIGSCTNSRIEDLRAAAAVVKGYQVAASVKQALVVPGSGPVKRA"
     gene            1326..2225
                     /locus_tag="PKI23_10565"
     CDS             1326..2225
                     /locus_tag="PKI23_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNN37419.1"
                     /translation="METQLLRAFLTVADQQSVSKAAERLHLTQPAISKQVQSLESQLG
                     IALFDRIGRRLNLTEAGRIFMPRAQAILQQLEESQRELRNLHAQVSGPLTLATSHHIG
                     LHRLPPVLRRYAEAYPQVDLQIEFLDSEAAYAQVLQGRFELAVITLAPEIDPRLDARL
                     LWSDRLRFVAAPHHPLASRRRLSLQQLAQHRATLPDLSTYTGQIVERLFAGAGIRLNR
                     GMATNYLETLKMMVSIGLGWSLLPQTMVDAGMVPLDVAVPPIERPLGVILHRARSLSN
                     AARIFLELLIDSADRSEAGGNRG"
     gene            complement(2199..3296)
                     /gene="aroC"
                     /locus_tag="PKI23_10570"
     CDS             complement(2199..3296)
                     /gene="aroC"
                     /locus_tag="PKI23_10570"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNN37420.1"
                     /translation="MSGNTLGKLFTVTTFGESHGPALGAIVDGCPPGLALSEADLQVD
                     LDRRRPGTSRFTTQRREPDQVRILSGLFEGVTTGTPIGLLIENSDQKSRDYQAIAEQF
                     RPAHADYTYFMKYGRRDHRGGGRASARETAMRVAAGAIAKKYLAERAGVRVRGYLSQM
                     GEIRPQRFDWDAVEQNPFFFPDPDQVPQLEALITQLRDEGDSIGAKVTVVASGVPVGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGSGFEVVTQRGSSHRDELTPQGFLSNHAGGI
                     LGGISSGQEIIAHLALKPTSSIRIPGRSIDVRGTPVEVVTTGRHDPCVGVRATPIAEA
                     MLAIVLMDHLLRHRGQNGDVEPGLAPIPASL"
     gene            3442..4158
                     /locus_tag="PKI23_10575"
     CDS             3442..4158
                     /locus_tag="PKI23_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN37421.1"
                     /translation="MAEPTRRKRKHPPKKQPRLGARLKFWLQCALAFFILLLPVAVWL
                     WYSQPHSAEGYYQKAIAEQTEEKTKEAIIDLRLGLKLDPDHIDARILLGELLLRQGAI
                     QSAEKEARMLLQLDGGNRRAQALLHNVTLAQQGVITDSQSGRFQDLKTVEQVELLVRL
                     GNGLLDQSHPETAIEQFESALKIDPDSRAAHLGLARAALLRGDERQFTAHLTTLVDLS
                     RRQAASQTNETGAKNTDSTR"
     gene            4207..>5234
                     /locus_tag="PKI23_10580"
     CDS             4207..>5234
                     /locus_tag="PKI23_10580"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNN37422.1"
                     /translation="MPSKKIPNRPQRRNSKMLLWSAGGGLLLTGLLASGAWIWFSQPR
                     SAEQYYQRALTERDQGQIKEAIIDLKNSLKLDPRQPNPRILLGELQLRQGDLQSAEKE
                     LRTVLQIDGNNERAQALLLDLTSGQHSSDNQKIRLEGLKPQEQVDLLVASGNGLLSKN
                     QADNASKQFEEALRIDPNSLGAYLGLAHAALLRGDTEQFTTHLAKLESLDSKNAQVAL
                     LRGDWKLRQGDVAGAEQDYSTAIAQDARMVEAIIKRGLVRVQQKRPAEVRQDAKRLAE
                     IEPKHPAAPLLFGMAAQQENNLNEAEQQFRAALQQGDYLPAWRQLALLQLQKKSYPEA
                     EISLRKVLG"
BASE COUNT         1020 a         1750 c         1611 g          853 t
ORIGIN      
        1 gcacgcttga ccggacccga accgggcacc accagtgcct gcttgaccga cgccgccacc
       61 tggtagccct tcaccacggc tgcggcggcg cgcagatcct cgatgcggga gttggtgcag
      121 gagccgatga agacccgatc gaccgcaatc tcgctgatcg gctggccggc acgcagcccc
      181 atgtattcga gcgcccggcc gtagccttca cggcgcaccg catcgctctg ggcagcggga
      241 tcgggaatgg ccgcatcgat cggcagcacc atctcgggcg acacccccca ggagacctgt
      301 ggcttgactg cggccgcatc gaactcgaaa caggcatcga agcgggcatc ggcatcgctg
      361 atgagcgtgc gccagtgggc cactgcctgc tgccacagct cgcccttggg cgcatagggg
      421 cgcccctgca ggtagtcgag ggtggtctga tcgaccgcca ccagcccggt gcgcgcgccg
      481 gcctcgatcg ccatgttgca gagggtcatc cgtccctcca tgctcaaggc ccggatcgcg
      541 ctgccgctga attcgatcgc atagccggtg ccgccggcgg tgccgatcgc gccgatgatg
      601 tgcagcacca gatccttggc ggtcacgccg ggcgccaggg tgccgctgac ctcgatgcgc
      661 atgttcttca gctttttctg caccagacac tggctggcca gcacatgctc gacctcgctg
      721 gtgccgatcc cctgcgccag cgccgcgaag gcgccatggg tcgaggtgtg ggagtcgccg
      781 cagaccacgg tcatgcccgg cagggtcgca ccctgctccg gcccgatgac atggacgatg
      841 ccctgccgcg gatcgttcat cctgaactcg gtcaggccaa attcgcggca gttgtcatcc
      901 agcgtctgca cctggagccg cgagatcgga tcgacgatgc cggccacgcc actgctgcgc
      961 tcggtggtcg gcacattgtg gtcgggcgtt gccagcaccg aatcggcccg ccacggttta
     1021 cgaccggcca ggcgcaatcc atcgaaggcc tgtgccgagg tgacttcatg cacgatgtgt
     1081 cgatcgatgt agatcagcgc ggaaccgtca tcccgctcga ccaccacatg gtcgttccac
     1141 aatttgtcgt acagcgttgc tccgcccatt gttcaccgcc ccgttgcgcg tctggttgag
     1201 gattcgatgc cggagcagcc tgtaaacggt gctccgctgg actgaatgct agggcctcgt
     1261 ttcaaataac acaaattcat gttattcatg aaaccgattc catttcatca ttcaaattac
     1321 tccacatgga aacccaactg ctgcgcgcct ttctgacggt ggccgaccaa cagtcggtct
     1381 cgaaagccgc cgaacggctg catctgaccc agccagccat cagcaaacag gtgcagtcgc
     1441 tcgaaagcca gctcggcatc gccctgttcg accgcatcgg ccgccgactc aaccttaccg
     1501 aggccggtcg catcttcatg ccgcgcgccc aggcgattct gcaacagctg gaagagagtc
     1561 agcgcgaact gcgcaacctg catgcgcagg tgagcggccc gctgacgctg gccaccagtc
     1621 atcacatcgg attgcaccgg ttgccgccgg tgctgcgccg ctatgccgag gcctatccgc
     1681 aggtcgatct gcaaattgaa tttctcgact ccgaggccgc ctacgcgcag gtgctgcagg
     1741 ggcggttcga actggcggtc atcacgctgg cgcccgaaat cgacccccgc ctcgatgccc
     1801 gcctgctctg gtccgaccgg ttgcggttcg tggccgcgcc gcaccatccg ctggccagcc
     1861 gcaggcggtt gtcccttcag caactggcgc agcaccgcgc caccctgccc gacctgtcga
     1921 cctacaccgg acagatcgtc gagcggctct ttgccggcgc aggcatccgg ctgaatcggg
     1981 gcatggccac caactatctg gagaccctga agatgatggt gtcgatcggg ctgggctgga
     2041 gtctgctgcc gcagacgatg gtcgatgccg gcatggtgcc gctcgatgtc gcggtgccac
     2101 caatcgagcg cccgctcggg gtcatcctgc accgcgcgcg gtcactctcc aatgcggccc
     2161 gaatctttct ggagctgctg atcgacagtg ccgaccgctc agaggctggc gggaatcggg
     2221 gctagaccgg gctcgacatc gccattttga ccccggtggc gcagcaggtg gtccatcagg
     2281 acgatcgcca gcatcgcctc ggcaatcggt gtggcgcgca cgccgacaca gggatcatgg
     2341 cgcccggtgg tgaccacctc gaccggcgtg ccacgcacat cgatgctgcg tccgggaatg
     2401 cggatgctgg aggtgggctt gagcgccaga tgggcaatga tctcctgacc gctggagatg
     2461 ccacccagga tgccaccggc atggttgctg agaaaaccct gcggcgtcag ttcgtcacgg
     2521 tggctgctgc cacgctgcgt caccacctcg aaaccgctgc cgatctccac ccccttgacc
     2581 gcattgatgc tcatcaatgc atgggcgatg tcggcatcga gccggtcgaa caccggttcg
     2641 cccagcccca ccggcacccc gctggccacc accgtcacct tggcgccgat cgagtccccc
     2701 tcgtcacgta gctgggtgat cagcgcttcc agttgcggca cctgatcagg atcgggaaag
     2761 aaaaagggat tctgctcgac ggcgtcccaa tcaaaacgct gcggccggat ctcgcccatc
     2821 tggctcagat agccgcgcac ccgcacgccg gcccgctccg ccagatattt cttggcgatg
     2881 gcgccggccg ccacccgcat cgcggtctcg cgcgccgagg cccggccacc gccacggtgg
     2941 tcgcggcggc catacttcat gaagtaggtg tagtcggcgt gggccggacg gaactgctcg
     3001 gcaatcgcct ggtagtcgcg tgacttctgg tcgctgtttt cgatcagcag gccaatcggc
     3061 gtgccggtgg tcacaccttc gaagaggccg gaaagaatcc tgacctgatc cggttcgcgt
     3121 cgctgggtgg tgaagcgtga agtgccggga cggcgacggt ccagatcgac ctgcagatcg
     3181 gcctcgctga gcgccaaccc cggcggacag ccatcgacga tcgcgcccag tgccgggccg
     3241 tggctctcgc caaaggtggt gacggtgaaa agtttgccca gggtgttgcc agacatggac
     3301 gcgatcgcct ctgctatgac caaagcgcca tgataccccg atcgcgccag cgaccgaagg
     3361 caaggcaacc aggcgcttac aaccgcagca ggctgatcta cactcaaccc catcgacact
     3421 gtggccactt cgagcgcacc gatggctgaa ccgaccagaa gaaaaagaaa acacccccca
     3481 aaaaagcaac cgcgactggg cgccaggctg aaattctggc tgcagtgtgc gctggcgttc
     3541 ttcattctgt tgctgccggt cgcggtctgg ctctggtact cccagccgca cagtgccgaa
     3601 ggctactatc agaaggccat cgcagagcag accgaggaga agaccaagga ggcgatcatc
     3661 gacctgcgac tcggcctcaa gcttgacccc gaccacatcg atgccagaat cctgctcggt
     3721 gaactgctgt tgcggcaggg tgccatccag tccgccgaga aggaggcgcg catgctgctg
     3781 caattggatg gcggcaaccg acgcgcgcag gcactgctgc acaatgtcac gctggcgcag
     3841 cagggtgtca tcaccgacag ccagagcggt cgctttcagg atctgaaaac cgtggaacag
     3901 gtcgaactgc tggtgcgcct tggcaatggc ctgctcgatc agagccatcc ggagactgcc
     3961 atcgagcagt tcgaaagtgc gctgaagatc gatcccgatt cacgcgcggc ccacctgggg
     4021 ctggcgcgcg ccgcgctgct gcggggtgat gaacggcagt tcactgcaca cctgaccacg
     4081 ctggtcgatc tgagcagacg gcaggcagcg agccagacga acgaaaccgg cgccaaaaac
     4141 accgacagca cgcggtaaca cgtcaacaac gccacctcga cagcaaaggg acgacaggaa
     4201 cagcccatgc ccagcaaaaa aatcccgaac agaccacaac gacgcaacag caaaatgctg
     4261 ctctggtcag ccggcggcgg cctgctgctg accggcctgc tggcgagcgg cgcctggatc
     4321 tggttctcgc agccacgcag tgccgagcag tattaccagc gggcgctcac cgagcgtgat
     4381 cagggccaga tcaaggaggc catcatcgac ctcaagaaca gcctcaagct cgacccccgg
     4441 cagcccaatc cgcgcattct gctgggcgag ctgcaactgc gtcagggtga cctgcaatcg
     4501 gccgaaaaag agctgcggac ggtgttgcag atcgacggca acaacgagcg ggcgcaggcg
     4561 ctgctgctcg acctcaccag tgggcagcac agcagcgaca accagaagat caggctcgaa
     4621 gggttgaaac cgcaggagca ggttgacctg ctggtggcct cgggcaatgg tctgttgagc
     4681 aagaaccagg ctgacaacgc cagcaaacag ttcgaggagg ccctgcggat cgatcccaac
     4741 tcgttgggcg cctatctggg actggcccac gccgcgctgc tgcgcggcga caccgagcaa
     4801 ttcaccaccc acctggccaa actggagtct ctggacagca aaaacgccca ggtggccctg
     4861 ctgcgtggcg actggaaact gcgccagggc gacgtggccg gcgccgagca ggactattcg
     4921 accgccatcg cccaggatgc gcgcatggtc gaggcgatca tcaagcgcgg actggttcgc
     4981 gttcagcaaa agcgaccggc ggaggtccgg caggatgcca aacgactcgc cgaaatcgaa
     5041 cccaaacatc ccgctgcacc actgctgttc ggcatggccg cccagcagga gaacaacctc
     5101 aacgaggccg aacagcagtt ccgggccgcc ctgcagcagg gcgactacct gccggcctgg
     5161 cgccagctcg cactgctgca actgcagaaa aagagctatc cggaggccga aatttcgctg
     5221 cgcaaggtgc tcgg
//
LOCUS       DAORCQ010000129         5119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_12085_length_5119_cov_3.394747, whole genome shotgun sequence.
ACCESSION   DAORCQ010000129 DAORCQ010000000
VERSION     DAORCQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12085_length_5119_cov_3.394747"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1298)
                     /locus_tag="PKI23_10585"
     CDS             complement(240..1298)
                     /locus_tag="PKI23_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNN37423.1"
                     /translation="MSIQVESLHKKFGDFTALDNVTLEFPSGELVALLGPSGCGKTTL
                     LRIIAGLESPDSGSVAFFGEETTQLHVRERHVGFVFQHYALFRHMTVFDNVAFGLSVR
                     PKKHRPDKATIVRRVHELLELVQLDWLADRYPHQLSGGQRQRIALARALAVEPRVLLL
                     DEPFGALDAKVRKDLRRWLRTLHDELHITSIFVTHDQEEAMEVADRIVVLNKGRIEQT
                     GTPDEIYNDPATPFVYDFIGHVNLFHSRVDHGWAHIGDYRVAAPEHHDIRDQAAVAYV
                     RPHDIELSEQPSEGAMAARIVHVSTAGSVLRIELQSQHNSDLLHVELPRAQQRSLNLT
                     VGAQVFARPTSSKVFLQG"
     gene            complement(1295..2224)
                     /gene="cysW"
                     /locus_tag="PKI23_10590"
     CDS             complement(1295..2224)
                     /gene="cysW"
                     /locus_tag="PKI23_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNN37424.1"
                     /translation="MGGTVSSLGLGRRGSAGFAIREPAWVRGTLTAIALVFLALFLLL
                     PLILVFSEAFSRGLEVYLAAISEPAARHAIWLTLVAAAIAVPCNLIFGLAAAWAIAKF
                     DFRGKQLVITLIDLPFAVSPVIAGLIYMLLFDRQGLLGAWLSQHDIQIMFALPGIVLA
                     TMFVTFPFIARELIPLMQEQGREQEEAAITLGASGWKMFRHVTLPSIKWGLLYGLILC
                     NARAMGEFGAVSVVSGKIREQTSTLPLHIEILYNEYQFAAAFATASLLTLLALGTLVL
                     KSLLEWRSAREHTLVEHGAPLTEFKKSSKGGVR"
     gene            complement(2227..3114)
                     /gene="cysT"
                     /locus_tag="PKI23_10595"
     CDS             complement(2227..3114)
                     /gene="cysT"
                     /locus_tag="PKI23_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNN37425.1"
                     /translation="MAEITPVALAAAPPSFTRSQRRWVSRSALPGFGLSLGFATFYAS
                     LIVFIPLAGLLLKSMELSLPELWSVISNPRSLASYRLTFGTALIAAALNGCFGLVVAW
                     VLVRYDFFGKRVVDALVDLPFALPTAVAGIALTALYAPDGWVGRWFAMAEIKIAYTWL
                     GITVALTFIGLPFVVRTVQPVLADLEAEIEEVAASLGASRWQTFSRVILPALLPSLLT
                     GFALAFARGVGEYGSVVFISGNMPYRTEITPLLIMTKLEQFDYAGAAALGMTLLLFSF
                     IFLLLINLLQHWSHRRAGG"
     gene            complement(3163..4158)
                     /locus_tag="PKI23_10600"
     CDS             complement(3163..4158)
                     /locus_tag="PKI23_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNN37426.1"
                     /translation="MKLIEKLSAALLIGLLASSATAKEIKLLNVSYDPTRELYREFNA
                     AFARHWKERSGDSVSIDQSHGGSGAQSRAVIDGLKADVVTLALAGDIDAIAKSGRLLP
                     NEWIGRLPNNSAPYTSTIVFLVRKGNPKQIKDWNDLARAGVEVITPNPKTSGGARWNY
                     LAAWAFAEKKQGQNPAGAKEFVRAIYKNVPVLDTGARGSTTTFVRRGIGDVLITWENE
                     AFLAINELGADKVEIVVPSFSILAEPSVAVIDENAKANGTAEVARAYLEYLYSKEGQQ
                     IAARHHYRPRDSEVAAAHAGSFPKLDLVTIEEVGGWARVQAEHFGDGGIFDQIYQ"
     gene            complement(4184..>5119)
                     /locus_tag="PKI23_10605"
     CDS             complement(4184..>5119)
                     /locus_tag="PKI23_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNN37427.1"
                     /translation="DITLFYRLEYGIDVDNGTNSNGRELSQRNIYGGLRGDWGAVLAG
                     KNDTPLKSLQTNTIKRSDIDRFNDFPLGDIGTYIVGENRPDNVIQYSSPILFGGLEIN
                     VAAVQAEETGVAVSATNQQNDNGFASGRSVSLSYGKADWFVGVAADSNISTTDALRFV
                     GEITLGPVKLGDIYQTAEAHDDVDVLGGFSTFVGTSGTTVGAQNGLNPLSEWDGASGN
                     AFKKQRGHILNGQWKISGPWVGRVQYAHSSTTPSNASYADVDLDGYVLGLDYNLNATT
                     RLFIAAATVKTEGDERIGADAVTDSTAALGFDLRF"
BASE COUNT         1055 a         1548 c         1524 g          992 t
ORIGIN      
        1 cggcgtccat cgggtcggac ttggccgagt gctggctgag aatcttcagg ttcagccgct
       61 tcggccacca gttgctgttg gactgcgcag ccgcggtggt gcggctgccg tggaaggggc
      121 atttgggctc gttggacatg gcgtgctcct gggtctttgg ctatgagagt tgccaatcta
      181 ctcaagctct ttccgtggtt ccaattgatt ttttggatgc ggtcgatagc aaagttctat
      241 cacccctgca agaagacttt cgaactggtc gggcgcgcaa acacttgggc gccaacggtc
      301 agattgagcg aacgctgctg ggcccgcggc agttcaacat gcagcagatc actgttgtgc
      361 tgcgattgca attcgatgcg cagcaccgaa ccggccgtgc tgacatggac gatccgcgcc
      421 gccatggcgc cctcgcttgg ctgttcgctg agttcgatgt catgcggccg cacataggcc
      481 accgccgcct gatcgcgaat gtcgtgatgc tcgggtgccg ccactctgta atcgccaatg
      541 tgcgcccagc catggtcgac acggctgtgg aacagattca catggccaat gaagtcatac
      601 acgaatggcg tggccggatc gttgtagatc tcgtccggtg tgccggtctg ttcgatgcgc
      661 cccttgttga gcacgacgat ccgatcggcc acctccatcg cctcttcctg gtcatgggtg
      721 acgaaaatcg aagtgatgtg cagctcatca tgcagtgtgc gcagccagcg ccgcagatcc
      781 ttgcggacct tggcatccag ggcgccaaag ggctcatcca gcaagagcac cctcggctcg
      841 accgccagtg cgcgggccag ggcaatgcgc tggcgctgtc cgcccgacag ttggtgggga
      901 tagcggtcgg ccagccagtc gagctggacc agttcgagca actcatgcac ccgtctgaca
      961 atggtggctt tgtccggccg atgttttttg ggacgcacac tgagaccgaa tgccacattg
     1021 tcgaagaccg tcatgtgtcg gaacagcgca tagtgctgaa acacgaaacc gacatgacgt
     1081 tcgcgcacat gcaactgggt ggtctcttcg ccaaagaacg caacactgcc cgaatcgggg
     1141 ctttccagcc cggcaatgat gcgcagcaag gtggtcttgc cacagccgga agggcccagc
     1201 agtgcaacca attcgccact ggggaactcc aacgtgacat tgtcgagggc agtgaaatcg
     1261 ccgaatttct tgtgcagact ttcaacctga atgctcatcg aactccccct tttgaactct
     1321 ttttgaactc tgtcaatgga gcaccgtgtt ccaccaaggt atgttctcgc gccgatcgcc
     1381 attccagcag gcttttcagc accagcgtgc ccagtgccaa cagggtcagc agtgatgcgg
     1441 tggcgaaggc ggcggcgaat tgatattcgt tgtacagaat ctcgatgtgc aggggcagtg
     1501 tgctggtctg ctcgcgaatc ttgccggaaa ccacgcttac cgctccgaac tcccccatgg
     1561 ccctggcatt gcaaagaatc agcccgtaga gcaatcccca tttgatcgaa ggcagggtga
     1621 catggcggaa cattttccag ccgctggcgc ccagcgtaat ggccgcctcc tcctgctccc
     1681 tgccctgctc ctgcatcagt ggaatcaact cccgggcaat gaatggaaag gtcacgaaca
     1741 tcgtcgccag cacgatgccg ggaagcgcga acatgatctg aatgtcatgc tgactgagcc
     1801 aggctcccaa caggccctga cgatcgaaca gcaacatgta gatcaggccc gcgatcaccg
     1861 gtgagactgc aaagggcaga tcgatcaggg tgatgaccaa ctgcttgcca cgaaagtcga
     1921 acttggcgat ggcccaggcc gccgccagac cgaagatcag gttgcagggc accgcgatcg
     1981 ctgccgcgac caaggtgagc cagatggcgt ggcgtgccgc cggttcgctg atcgccgcga
     2041 gatagacttc cagacccctg gagaaggcct cgctgaagac gaggatcagc ggcaaaagca
     2101 gaaacagggc caaaaaaacc agcgcaatgg cagtcagcgt gccgcgcacc caggctggtt
     2161 cgcggattgc gaaccctgca ctgcctcgcc gccccagacc cagtgatgaa accgtgccgc
     2221 ccatgttcaa cctccggcgc gtcggtggct ccagtgctgg agcaggttga tcaacaacag
     2281 aaaaatgaat gaaaacagca acagggtcat gccgagcgcc gcggcaccgg cgtaatcgaa
     2341 ctgctcgagc ttggtcatga tcagcagcgg cgtgatttcg gtgcggtacg gcatgttgcc
     2401 gctgatgaag accaccgagc cgtattcacc aacgccccgg gcaaaggcca gggcaaagcc
     2461 ggtcagcagt gagggcagca gtgccggaag gatgacgcga ctgaaggtct gccagcgtga
     2521 agcgccgagg ctcgctgcca cctcctcgat ctcggcctcc agatcggcca ataccggttg
     2581 cacggtgcgc acgacgaagg gcagaccaat gaaggtcagc gccaccgtga tgccgagcca
     2641 agtgtaggcg atcttgatct ccgccatggc aaaccagcgc ccgacccagc catccggtgc
     2701 gtaaagcgca gtcagggcaa ttccggccac ggccgtcggc agtgcaaagg gcaagtcgac
     2761 cagtgcatcg accacccgct ttccaaagaa gtcatagcgc accagtaccc aggccaccac
     2821 cagtccgaag cagccattga gagcggccgc gatcagtgcc gtgccgaagg tcagccggta
     2881 actggccagc gagcgagggt tgctgatgac actccacaac tctggcaagc tgagctccat
     2941 cgacttgagg agcagcccag ccagcggaat gaacacgatc aggctggcat agaaggtggc
     3001 aaagcccagc gaaaggccga agcccggcag ggccgatcgg ctgacccagc gccgctggct
     3061 gcgggtgaag gaaggtggcg ccgctgccag cgccacgggg gtgatttcag ccatcggtgg
     3121 tggtccagtt gcaggcgatg agggaagggt gagatcgatg gatcactgat aaatctgatc
     3181 gaagatgccg ccatcaccga aatgctccgc ctggaccctg gcccagccac cgacctcctc
     3241 gatggtgacc agatccagtt tcggaaaact tccggcatgg gcggcggcca cctcgctgtc
     3301 gcgcggccga tagtggtgtc ttgcagcaat ctgttgaccc tccttgctgt agaggtactc
     3361 cagataggcg cgggcgacct cggccgttcc attggccttg gcattttcat cgatgaccgc
     3421 caccgagggt tccgccagga tggagaagct cggcaccacg atctccacct tgtcggcacc
     3481 cagttcattg atcgccagaa aggcttcgtt ctcccaggtg atcagcacat caccaatgcc
     3541 acgcctgacg aaggtggtgg tcgaaccgcg ggcaccggtg tccagcaccg gaacgttctt
     3601 gtagatcgcg cggacgaact ccttcgcacc tgccgggttc tgtccctgct tcttttccgc
     3661 gaaagcccag gccgccagat agttccagcg cgcgccgccc gaggtcttgg ggttgggggt
     3721 gatgacctcc acaccggctc tggccaagtc attccaatcc ttgatctgtt tggggttgcc
     3781 cttgcggacc agaaagacga tggtcgaggt gtagggtgcg gagttgttcg gcaatcggcc
     3841 gatccactcg ttcggcagca gcctgcccga tttggcgatg gcatcgatat cacccgccaa
     3901 ggccagcgtg accacatcgg ccttcagtcc atcgatcacg gcacgtgact gcgcacccga
     3961 tccaccatgc gattgatcga tgctgacgct gtcgccgctg cgttccttcc aatgcctggc
     4021 gaaggccgcg ttgaattccc gatagagttc ccgcgtcggg tcataggaga cgttcagcag
     4081 tttgatctcc ttcgccgtcg cgctgctggc cagaagaccg atcaacaggg ccgcactcaa
     4141 tttttcaatc agtttcatca gactgttctc cagagtcgtg tgatcaaaaa cgcaggtcga
     4201 agccgagcgc cgcagtggaa tcggtgacgg catccgcccc gatgcgctca tctccctcgg
     4261 ttttcaccgt ggcggcggcg atgaagaggc gggtcgtggc attgaggtta taatccagac
     4321 ccaaaacgta cccatccagg tcgacatcgg catagctggc attgctcggc gtggtgctgg
     4381 agtgggcata ctggactcgg cccacccaag gcccggaaat cttccactgc ccattcagaa
     4441 tgtgcccacg ctgttttttg aaggcgttgc ccgatgcacc atcccattcg gagagcggat
     4501 tgaggccatt ctgcgccccc accgtcgtgc cgctggtgcc gacgaaggtc gaaaaaccac
     4561 cgagaacatc cacatcatca tgggcctctg ccgtttgata gatgtctccc agtttcaccg
     4621 gacccagggt gatttcccct acgaaacgca aggcatcggt ggtcgagatg ttggagtcgg
     4681 cggcaactcc cacgaaccag tcggccttgc cgtaactcag cgagaccgat cgacccgagg
     4741 caaagccatt gtcgttctgc tggttggtgg cactcaccgc caccccagtc tcttctgcct
     4801 ggacagcggc gacattgatc tcaagccccc cgaacagaat cggtgaactg tattggatga
     4861 cattgtcggg acggttttcg ccaacgatgt aggtgccaat gtcacccagc ggaaaatcgt
     4921 tgaagcggtc gatgtcgctg cgcttgatcg tgttggtttg caggctcttg agcggggtat
     4981 cgttcttgcc ggcgagaacc gcaccccagt cgccgcgcag gccgccgtag atgttgcgct
     5041 ggctcagctc gcgcccattg ctgttggtgc cattgtcgac gtcgatgcca tattccagac
     5101 ggtaaaagag ggtgatgtc
//
LOCUS       DAORCQ010000130         5090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_12206_length_5090_cov_5.749752, whole genome shotgun sequence.
ACCESSION   DAORCQ010000130 DAORCQ010000000
VERSION     DAORCQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12206_length_5090_cov_5.749752"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="cheB"
                     /locus_tag="PKI23_10610"
     CDS             complement(<1..843)
                     /gene="cheB"
                     /locus_tag="PKI23_10610"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNN37428.1"
                     /translation="MSPTRTRVLIVDDSAVVRQVLTALLNRERSIEVVGAASDPIFAL
                     ERMNRDWPDVVLLDLEMPRMDGLTFLKKIMATRPTPVVICSTLTEKGAEISMQALAAG
                     AVSIVTKPKAGLKGFLQDDIGSIVTAIRSAAAADMQRLKKSTSQLAALPVPAKLSVDA
                     LLPAADGAAPLLTTTTERIVAIGTSTGGTQALEVVLTALPRVAPGIVIVQHMPEKFTA
                     AFAERLNGLSAIEVREARHGDRVVPGRALIAPGGRHMTLKRSGAQYHVEVIDGPPINR
                     HKPSV"
     gene            complement(840..1367)
                     /locus_tag="PKI23_10615"
     CDS             complement(840..1367)
                     /locus_tag="PKI23_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337790.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNN37429.1"
                     /translation="MKNPVSRQPPARPVEIFLNPGEFHFGHRDTRIRTLLGSCVAITL
                     WHPQLLIGGMCHYLLPSAGPGSSSAELDGRYADQAMLRFMRELARSDTRPEQYQVKLF
                     GGGNQFPELLQDSAGGKVPERNIEAGQRLLADYGFRLEAQHVGGTGHRTLLLELWSGH
                     VWLRHVAGTASGGVA"
     gene            complement(1364..2158)
                     /locus_tag="PKI23_10620"
     CDS             complement(1364..2158)
                     /locus_tag="PKI23_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019360488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNN37430.1"
                     /translation="MRPDEFEWIRRFLHQQSGIALNDSKRALVTGRLEKRLRHHRLGS
                     YGDYFRLLGQPGQQAEAALVVDLLTTNETFFFREPKHFEFLREQLLASHPRNRPLRCW
                     SAASSSGEEAYTLAMLLAEHGPSEWEVIGTDISGRVIEKAQRGLYPLAAADKIPLPLL
                     KKYCLKGHDEYEGLLRIQDTLRARVRFLRANLLQPLPELGLFDVILLRNVMIYFDMPT
                     KQRLLDRLQPLLRPGGYFIISHSETLNGISTALKPVQPSIYRSAGA"
     gene            complement(2197..2697)
                     /locus_tag="PKI23_10625"
     CDS             complement(2197..2697)
                     /locus_tag="PKI23_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNN37431.1"
                     /translation="MESGQYLTFMLAGETFALGILNIKEIIKYGALTTVPLMPDFIRG
                     VINLRGRVVPVVDLAARFGRASTRVARRTSIVIIETRVDEGEQADGELQEIGILVDAV
                     NEVVEIDGDAIRPPPTFGAGVQPEFIHGMAQRGSGFVILLDLNHLLSDDQVALLGQEV
                     APCLSE"
     gene            complement(2767..>5090)
                     /locus_tag="PKI23_10630"
     CDS             complement(2767..>5090)
                     /locus_tag="PKI23_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNN37432.1"
                     /translation="KMTIRAKLIFLLAIAIGITVVVGTIAVLGLRDTTRAMHEIGSVR
                     LPSVVGLHMISEGQTAIRSANRGVLVLQNSPKAREQFAAILKEKQNIWARIDDGWKLY
                     EPLPQTEEEAVLWKRFVQEWQLWRAADNRMSETIEALSKGQSDEGQKVLFAKMESQLR
                     EVLPLFGAAQTTLREISDLNERVGTQAVATGEADATRATTLMLVSALGATALLILFAA
                     VVIRGIRRPLSAAVVFFDRLASGETDLEIETGHDEIGQMLKAFDAVRARMKADAEVMA
                     QMLAENSRIRIALDNVTTNVMIADNDRNIVYTNQSVIDMLRNAESDIRKELTNFNAGR
                     LIGSNIDQFHKHPEHQRQMLATFSSTHRAQIKIGGRTFGLIANPVISAQGERLGSVVE
                     WKDRTDEVAIEGEVARIVDAASSGDLTRRIDLAGKEGFFRALAGSINGMVSNTEAIIN
                     ETVGALERMARGDLTAPIDGSYQGSYGVIQQGYNDTMNRLSQIIGDVMTAADQLANAS
                     GQISATSQALSQAASEQAASVEETSASVEQMSASINQNAENAKVTDGMASKAAQEANE
                     GGGAVRQTVEAMKQIAERIGIIDDIAYQTNMLALNAAIEAARAGEHGKGFAVVAAEVR
                     KLAERSQVAAQEIGELAGGSVRMAERAGELLAEIVPSIGKTSDLVQEIAAASQEQSSG
                     ASQINMAMSQMNQITQQNASSSEELAATAEEMTSQAEQLQTLMDFFKLAGHDSALRGL
                     ASPVPPTRGRGMSPRPAAAVAEAGIDEAKFSRF"
BASE COUNT          848 a         1704 c         1570 g          968 t
ORIGIN      
        1 gaccgagggt ttgtggcggt tgatcggcgg accgtcgatc acctcgacat ggtactgggc
       61 cccgctgcgc ttgagcgtca tgtgccgtcc acccggcgcg atcagcgcgc ggcccggcac
      121 cacccgatcg ccatgccgcg cctcacgcac ctcgatggcc gacagcccgt tcagccgctc
      181 ggcaaaggcc gcagtgaact tttccggcat gtgctggacg atgacgatgc ccggcgccac
      241 ccttggcagc gcggtcagca ccacttccaa tgcctgagtg cccccggtgg aggtgccgat
      301 ggccacgatc cgctcggtgg tggtggtcag caacggcgcc gcgccatcgg cagcgggcag
      361 cagcgcatcg accgacagct tcgccggtac cggcagcgct gccagttggc ttgtagactt
      421 tttcagccgc tgcatgtcag cggccgctgc gctccggatg gcggtgacga tgctgccgat
      481 gtcatcctgc agaaaaccct tgagcccggc cttgggcttg gtgacgatgc tcaccgcgcc
      541 ggccgccagc gcctgcatcg agatctccgc ccccttctcg gtcagtgtcg agcagatcac
      601 caccggcgtc ggtcgcgtcg ccatgatctt tttcagaaag gtgaggccat ccatgcgtgg
      661 catctccaga tcgagcagca ccacatcggg ccagtcgcgg ttcatccgct ccagcgcgaa
      721 gatcgggtcg gacgcggcac cgaccacctc gatcgaccgc tcgcggttca gcagggcggt
      781 cagcacctgc cgcaccacgg cggagtcatc gacgatcagc acccgggtcc tggtcgggct
      841 catgcgacac ctccgctggc tgtgcctgcg acatgcctga gccagacgtg gccactccac
      901 agctcgagca gcagcgtgcg gtggccggtg ccgccgacat gttgtgcctc gagcctgaag
      961 ccatagtcag ccagtaaccg ctggcccgcc tcgatgttgc gctccggcac cttgcccccc
     1021 gcgctgtcct gcagcagttc gggaaactgg ttgccgccac cgaacagctt cacctgatac
     1081 tgctccggtc gggtgtcgct gcgggccagc tcgcgcatga accgcagcat ggcctggtcg
     1141 gcatagcggc catccagctc agccgagctg ctgcccggcc cggcgctcgg cagcagatag
     1201 tggcacatgc cgccgatcag cagttgcgga tgccacagcg taatggccac gcaggagccg
     1261 agcagcgtgc ggatgcgggt gtcgcggtgg ccgaagtgga attcgcccgg attcagaaag
     1321 atttcgaccg gccgtgccgg aggctgtcga gacaccgggt tcttcatgcg ccagcactgc
     1381 ggtagatcga tggctgcacc ggcttcagcg cggtactgat gccattgagc gtctcggagt
     1441 ggctgatgat gaagtagccg ccgggacgca gcagcggctg caacctgtcc agcagccgct
     1501 gcttggtcgg catgtcgaag tagatcatca cattgcgcag caggatcaca tcgaacagcc
     1561 ccagctcggg cagcggctgc agcaggttgg cgcgcagaaa acggacccgc gcgcgcagcg
     1621 tatcttgaat gcgcaacagc ccttcgtatt catcgtgccc tttcaggcag tattttttca
     1681 gcagcggtag cggaatcttg tcggcggcgg cgagcggata gagtccgcgc tgcgccttct
     1741 cgatgacccg accggagatg tcggtgccga tcacctccca ctcgctggga ccatgctccg
     1801 ccagcagcat cgccagcgtg taggcctcct cgccgctgga gctggccgca ctccagcagc
     1861 gcaacgggcg gttgcgcggg tgggatgcga gaagctgttc acgcaggaat tcaaaatgtt
     1921 tcggttcgcg aaagaaaaag gtctcgttgg tggtcagcag atcgaccacc agtgccgcct
     1981 ccgcctgctg gccgggctgt ccgagcaggc gaaaatagtc gccatagctg cccagccgat
     2041 ggtgccgcag ccgcttctcc agccgccccg tcaccaatgc ccgcttgctg tcattgagag
     2101 cgataccgct ctgctgatgc agaaagcggc gaatccactc gaactcgtca gggcgcaacg
     2161 cgggcgacgg attggcgcga tggaccgatg cctccttcac tccgacagac acggtgcaac
     2221 ctcctgcccc agcagcgcca cttggtcgtc cgacagcaga tggttcagat cgagcaggat
     2281 cacgaagccg ctgccgcgct gcgccatgcc atggatgaat tccggctgga ccccggcgcc
     2341 aaaggtcggc ggcggtctga tcgcatcgcc gtcgatctcg accacttcgt tgaccgcgtc
     2401 gaccaggatg ccgatctcct gaagttcgcc gtcagcctgt tctccctcat caacgcgggt
     2461 ctcgatgatg acgatgctgg tgcggcgcgc cacccgcgtg gacgcacggc caaagcgggc
     2521 tgccagatcg accaccggca ccacccggcc acgcaggttg atcaccccgc gaatgaagtc
     2581 cggcatcagc ggcacggtgg tcagggcacc gtacttgatg atctccttga tgttgagaat
     2641 gccgagggca aaggtctcac ccgccagcat gaaggtcagg tactggcccg actccatggc
     2701 ggcggcaggc gcgacgacgg cgggcagccg atccccggat gcagcgatgg ttttcatggc
     2761 gccctcctag aaccggctga atttggcttc gtcgatgccg gcctccgcca ccgccgcagc
     2821 agggcggggc gacatgcccc ggccacgggt cggcggcacg ggcgatgcca ggccgcgcag
     2881 cgcgctgtcg tggccggcca gcttgaagaa gtccatcaac gtttgaagct gctcggcctg
     2941 tgaagtcatc tcctcggccg tggcggccag ctcttcgctg ctggaggcat tctgctgggt
     3001 gatctggttc atctggctca tcgccatgtt gatctggctg gcaccggaag attgctcctg
     3061 cgaggccgct gcgatctcct gcaccagatc ggaggtcttg ccgatgctcg gcacgatctc
     3121 ggccagcaac tcaccggcgc gctccgccat cctgacactg ccgccggcca gctcgccgat
     3181 ctcctgcgcc gccacctggc tgcgctcggc cagcttgcgc acctcggctg ccaccacggc
     3241 gaaacccttg ccatgctcgc cggcacgggc cgcttcgatc gcggcattga gcgccagcat
     3301 gttggtctga taggcgatgt cgtcgatgat gccgatccgc tcggcgatct gcttcatcgc
     3361 ctccaccgtc tgcctgaccg cgccgccgcc ttcattggcc tcctgcgccg ccttgctggc
     3421 catgccatcg gtcaccttgg cattctcggc attctggttg atcgaggccg acatctgctc
     3481 gaccgaggcc gaggtctcct ccacgctggc cgcctgctcg ctggctgcct gcgacagcgc
     3541 ctgtgaggtg gcgctgatct gtccggaggc gttggcaagc tgatcggcag cggtcatcac
     3601 atcaccgatg atctgcgaca accggttcat ggtgtcgttg taaccttgct ggatgacacc
     3661 gtagctgccc tgatagctgc cgtcgatcgg tgcggtgaga tcacccctgg ccatgcgctc
     3721 cagggcaccg acggtctcat tgatgatggc ctcggtgttg ctgaccatgc cgttgatcga
     3781 tccggccagt gcccggaaga atccttcctt gcctgccaga tcgatgcgcc gggtcaggtc
     3841 accgctggac gcagcgtcga cgatccgcgc cacttcacct tcgatcgcca cctcatcggt
     3901 gcggtccttc cactccacca cgctgccgag gcgttcaccc tgggccgaga tgaccgggtt
     3961 ggcgatgagc ccgaaggtgc gtccaccaat cttgatctgg gcgcgatggg tgctgctgaa
     4021 ggtcgccagc atctgccgtt gatgctccgg atgtttgtgg aactgatcga tgttcgatcc
     4081 gatcagcctg ccggcattga agttggtcag ctccttgcgg atgtcggact ccgcattcct
     4141 gagcatgtcg atgaccgact ggttggtgta gacgatgttg cggtcattgt cggcaatcat
     4201 cacattggtg gtgacattgt cgagcgcgat cctgatccgg ctgttttcgg ccagcatctg
     4261 cgccatcacc tcggcatccg ccttcatgcg ggcacgcact gcatcgaacg ccttgagcat
     4321 ctggccaatt tcgtcatgac cggtctcgat ctcgagatcg gtctcaccgc tggccagccg
     4381 gtcgaaaaag acgactgccg cgctgagtgg tcgccggatg ccgcggatga cgaccgcggc
     4441 gaacagtatc agcaatgcag tggcgccaag agcgctgacc agcatcaggg tcgtggcacg
     4501 cgtcgcgtcg gcctccccgg tggccaccgc ttgggtgccc acccgctcat tcagatcgga
     4561 aatctcgcgc agcgtggtct gggcagcgcc aaagagcggc aacacttcac gcagttggct
     4621 ttccattttg gcaaagagca ccttctgccc ctcgtcggac tgccccttgc tcaacgcctc
     4681 gatggtctcg ctcatccggt tgtcggcggc tctccacagc tgccactcct ggacgaaccg
     4741 cttccacagc actgcctctt cttcggtctg cggcagcggt tcatagagct tccagccatc
     4801 gtcgatgcgc gcccagatgt tctgcttctc tttcaatatg gcggcaaact gctcccgagc
     4861 cttggggctg ttctgcaaca ccagcacgcc gcggttggcc gagcgtatcg cggtctgccc
     4921 ctcggagatc atgtgcagcc ccaccaccga aggcagccga acactgccga tttcatgcat
     4981 ggcgcgggtg gtgtcacgca gccccagcac tgcgatggtg ccgacgacaa ccgtgatacc
     5041 gatggcaatg gcgagcaaaa aaatcaactt cgccctgatg gtcattttgg
//
LOCUS       DAORCQ010000131         5087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_12218_length_5087_cov_5.043124, whole genome shotgun sequence.
ACCESSION   DAORCQ010000131 DAORCQ010000000
VERSION     DAORCQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12218_length_5087_cov_5.043124"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2690
                     /locus_tag="PKI23_10635"
     CDS             <1..2690
                     /locus_tag="PKI23_10635"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNN37433.1"
                     /translation="HLLLLRGWQPFCPETALQWPMALPALIQQLLYSASEVVPQLWLV
                     THGGALADGLPASATVQPHQGAVWGFGRVLMNECPMFDCTLIDIACDLEAADLAARLS
                     QELLQPDGASEIVLTESARHALCMQQATRQSVADPNGAARYRLDFHLPGKLKNLRWLP
                     AAEQPLAGHEIEVMPRAVGLNFRDVMYLMGLLPDEAVENGFAGASLGLEFAGVVSRVG
                     DAVQDLAPGDAVMGFGASCFASHVVTRAGAVARMPAGWSFASAATVPTVFFTVYYALK
                     HLADLQPGERVLIHGAAGGVGMAAIQLARHLGAEIFATAGSDEKRDFVRLLGADHVFD
                     SRTLAFADQIMEVTAGEGVDVVLNSLAGEAVRCNLRLLKPFGRFLELGKRDFFENTPI
                     GLRVFKENISYFGIDADQLLTARPALAERLFGEVMALFEQRVLTPLPQRSFAAERVVE
                     AFRVMQQSRHIGKIVVTLDAPPVRIESDAPLPQLQCGKEERWLISGGLSGFGLATAQR
                     LAARGVGHLLLLGRRGADTPDARQAVEALRTSGVEVTPLACDVTDAAAVAELIERIKQ
                     QGPPLTGIVHAAMSLDDGLIGSLDAQRMAQVLRPKLLGAWNLHRLTLDLPIRHFVLYS
                     SITTGIGNPGQANYVAANGALEVLARMRRAAGLPATCIGWGPIGDAGYLTRNEATKAQ
                     LEQRLGRPPLTAEQALDQLEQLLSEPCAGPVTVADFDWKVLSRLLPSAHASRFDELNR
                     QFAQKQQGMEQEIDFRTLLADRTPAEAHELLREVVLQQVAQILSLGAERIDRNRSLHE
                     LGMDSLMMVELALGLEQQFGIRLPVMLLNEAPTIEQVTARILDRLLADETSEASGAES
                     LDLLVQQMARQHGEVADVQEIAELANTAKALRDAGRVAG"
     gene            2687..4078
                     /locus_tag="PKI23_10640"
     CDS             2687..4078
                     /locus_tag="PKI23_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNN37434.1"
                     /translation="MSQTPGGGIDKQRLIHRVLDRRIGQERPPGATGVRRNSTSAQIP
                     DEFCRFDRHPQYEKIVVPKVAAERFGIDNPFFKAHDGVADAVTRIGGRPFINYSSYNY
                     LGLCGHPEVNRAAQQAIERYGTSASASRLVAGERPVQRELEQGIAELYGVEDCIVFVS
                     GHATNVSTIGYLFGPRDLVLHDQLIHNSVLEGIKLAGSARRAFAHNDTQALDALLSEL
                     RGQFERVLIVVEGHYSMDGDLPDLPALVEIKQRHKAFLMVDEAHSLGVLGRTGRGLHE
                     QCGVAASAVDIWMGTLSKSLAGCGGYIAGERALVEHLKYAAPGFVYSVGISPVLAAAS
                     LQALRIMQREPERVALLQARAQDFLRRARQAGLDTGHSQGFAVVPLLTGSSFEAVRLS
                     NRLFEQGINVQPIIHPAVEERAARLRFFISATHTEAQIATTIDLLTDLRSEATGIERQ
                     PATTPTTHPNDST"
     gene            4075..>5087
                     /locus_tag="PKI23_10645"
     CDS             4075..>5087
                     /locus_tag="PKI23_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HNN37435.1"
                     /translation="MKLSQRLPAALQRISPLFLATVVLPTLLALIYYGLLASDVYISE
                     SHFVIRSPERQSTSPLGFLLKGAGFARAEDDAHAVQNFMLSRDAVRALDGELGIKQAY
                     SAADLDLLSRFPGLDWDDSFENFHRYFQKQVTIQLDPTSSISILTVRAFSAQQAEAIN
                     RRLLELGEQLVNRLNERGRDDLIRYAAKEVSDLEAKAKGAALALAQYRNAQGVIDPER
                     QSSIPLQQIAKLQEELIATKAQRLQLERVARENPQLPLLQQRIQLLEQEIEAASMRVA
                     GGDRSLAGKAAEFQRLALEKEFADKMLASAMSTLELARNEAQRQQLYLERVAQPSLPD
                     QAL"
BASE COUNT          865 a         1652 c         1711 g          859 t
ORIGIN      
        1 accacctgct gttgctgcgt ggctggcagc cgttctgtcc cgaaaccgcg ttgcagtggc
       61 cgatggcgct gccggcactg atccagcagc tgttgtacag cgcgagcgaa gtcgtgccac
      121 agctgtggct cgtcacgcat ggtggtgccc tggccgatgg gctgcctgct tcggcaacgg
      181 tccagccgca ccagggggcg gtctggggtt tcggacgggt gctgatgaat gagtgcccga
      241 tgttcgactg cacgctgatc gacattgcct gcgatctgga ggccgccgat ctggcggcac
      301 ggctgagcca ggagctgctc cagcccgacg gtgccagtga aatcgtgctg accgaatcgg
      361 cgcgccatgc actgtgcatg cagcaggcga cccggcagag tgtggccgac ccgaacggtg
      421 cagcccgcta ccggctcgac ttccacctgc ccggcaagct gaaaaacctg cgctggctac
      481 cggctgccga gcagccgctg gccgggcatg agatcgaggt gatgccgcgg gcggtggggc
      541 tcaacttccg cgatgtcatg tacctgatgg gcctcctgcc cgatgaggcg gtcgagaatg
      601 gctttgccgg tgccagtctg gggctggaat tcgccggggt cgtcagccgg gtgggcgacg
      661 ccgtgcagga tctggcgccg ggcgacgccg tcatgggttt tggcgcctcc tgtttcgcca
      721 gccatgtcgt gacccgtgcc ggcgcggtgg cccgcatgcc ggcgggctgg tccttcgcca
      781 gcgcggccac ggtgccgacg gtcttcttta cggtctacta tgccctcaaa catctggccg
      841 atctgcaacc cggcgagcgg gtgctgatcc atggcgcggc cggtggcgtc ggcatggctg
      901 ccatccagtt ggcccgccat ctgggtgcgg agatctttgc cacggcgggc agtgacgaga
      961 agcgcgattt cgtgcggctg ctcggcgccg accatgtgtt cgattcgcgc acgctggcat
     1021 ttgccgatca gattatggag gtcactgcgg gcgaaggggt cgatgtggtg ctcaactcgc
     1081 tggcgggtga agccgtccgc tgcaatctgc ggctgctcaa gccattcggg cgttttctcg
     1141 aactcggcaa gcgcgacttt ttcgagaaca ccccgatcgg gctgcgtgtt ttcaaggaga
     1201 acatcagcta cttcggcatc gatgccgacc agcttctcac cgcgcgaccg gcactggccg
     1261 agcggctgtt cggcgaagtg atggcgctgt tcgagcagcg cgtgctgacc ccgttgccgc
     1321 agcgcagctt cgcggcggag cgggtggtcg aggccttcag ggtcatgcag cagtcccgcc
     1381 acatcggcaa gatcgtggtg acgctcgatg caccgcccgt gcggatcgag tcggatgcgc
     1441 cgctgccgca gttgcagtgc ggcaaagagg agcgctggct gatcagcggt ggtctgagcg
     1501 gcttcgggct cgccaccgcc cagcgactgg cggcgcgtgg cgtgggccat ctgctgctgc
     1561 tcggccggcg tggcgccgac accccggatg cacgacaagc ggtggaggcg ctgcggacca
     1621 gcggcgtcga agtcacgcca ctggcctgtg atgtcaccga tgcggcggca gttgccgagc
     1681 tgatcgagcg gatcaaacag caggggccac cgctcaccgg catcgtccat gccgccatga
     1741 gcctcgatga cgggttgatc ggcagcctcg atgcgcagcg catggcccag gtgctgcggc
     1801 ccaagctgct gggcgcctgg aacctgcacc ggctcaccct cgatctgccg atcaggcact
     1861 ttgtgctcta ctcatcgatc accaccggca tcggcaatcc ggggcaggcc aactacgtgg
     1921 ccgccaatgg cgcgctcgag gtgctggcgc ggatgcggcg ggccgccggc ctgccggcca
     1981 cctgcatcgg ttggggaccg atcggcgatg cgggttacct cacccgcaat gaggcgacca
     2041 aggcgcagct cgaacagcgg ttgggccggc cgccgctgac ggccgagcag gcgctcgatc
     2101 agttggagca actgctgagc gagccatgcg ccggtccggt gacggtggcc gatttcgact
     2161 ggaaggtgct gtcgcggctg ttgccttccg cccacgccag ccgttttgat gagctcaatc
     2221 ggcagtttgc gcagaagcag cagggcatgg agcaggagat cgattttcgc accctgctgg
     2281 ccgacaggac gcccgccgag gcccatgagc tgttgcgtga agtggtgctc cagcaggtgg
     2341 cgcagattct cagcctcggt gccgagcgca tcgaccgcaa ccgttcactg cacgaactcg
     2401 gcatggactc actgatgatg gtcgaactgg cgttggggct ggagcagcag ttcggcattc
     2461 gcctgccggt gatgctgctc aacgaagcgc cgacgatcga gcaggtgacg gcccgcatcc
     2521 tcgaccggct gttggccgac gaaaccagcg aggccagcgg cgccgaatcg ctcgatctgc
     2581 tggtacagca gatggccaga cagcatggcg aggtggcaga tgtgcaggag attgccgaac
     2641 tggccaacac ggccaaggcg ctgcgggacg ccgggagggt ggccggatga gccaaactcc
     2701 gggcggtggc atcgacaagc agcggctgat ccaccgtgtg ctcgacagac gcatcggcca
     2761 ggagcggccg ccgggtgcaa ccggcgtgcg gcgcaactcc acctcggcac agatacccga
     2821 tgagttctgc cgcttcgatc gccatccgca gtacgagaag atcgtcgtgc ccaaggtggc
     2881 ggccgagcgc ttcggcatcg acaacccctt cttcaaggcc catgacgggg tggccgatgc
     2941 agtgacccgc atcggtggcc gccccttcat caactactcc agttacaact acctcggtct
     3001 gtgcggccat cccgaggtca accgggcggc gcaacaggcg atcgagcgct acggcacctc
     3061 ggcctcggcc agccgcctgg tggcgggcga gcggccggtt cagcgcgagc tggaacaggg
     3121 catcgccgaa ctctatggcg tggaagattg catcgtcttc gtcagcggcc atgccaccaa
     3181 tgtcagcacc atcggctacc tgttcggtcc gcgcgatctg gtgctgcacg accagctcat
     3241 ccacaacagc gtgctcgaag ggatcaaact tgccggcagc gcgcgccgtg cctttgcgca
     3301 caacgacact caggcgctcg atgccctgct gagcgagctt cgcggacagt tcgagcgggt
     3361 gctgatcgtg gtcgagggcc actacagcat ggatggtgac ctgcctgatc tgccggcgct
     3421 ggtcgagatc aaacagcggc acaaggcctt tttgatggtg gatgaggcgc actcgctggg
     3481 cgtgctgggc cgcaccggcc gcggactgca cgagcagtgc ggcgtcgctg ccagcgcagt
     3541 cgacatctgg atgggcacgc tcagcaagag cctggccggt tgcggcggct acatcgccgg
     3601 cgagcgggcg ctggtcgagc acctgaagta cgcggcgccc ggtttcgtct acagcgtcgg
     3661 catctcgccg gtgctggcgg cggcctcatt gcaggcgctg cgcatcatgc agcgtgaacc
     3721 cgagcgggtg gcactgctgc aggcgcgggc gcaggacttt ctgcggcgtg cccgccaggc
     3781 ggggctcgat accggccatt cacagggttt tgcggtggtg ccgctgctca ctggcagctc
     3841 gttcgaggcg gtcaggttgt cgaaccggct gttcgaacag ggcatcaacg ttcagccgat
     3901 catccatccg gccgtcgaag agcgggcggc gcggctgcga ttctttatca gcgccaccca
     3961 caccgaggcg cagattgcca ccaccatcga cctgctcacc gatctgcgca gtgaagccac
     4021 aggcatcgag cgacagcctg caaccacacc gacaactcac ccgaacgatt cgacatgaag
     4081 ctttcacaac gtctgccagc cgccctccag cgcatcagtc cgctgtttct ggccaccgtg
     4141 gtcctgccga ccctgctggc actgatctac tacggcctgc tggcctcgga tgtctacatc
     4201 tccgagagcc atttcgtcat ccgcagcccg gagcgtcaga gtacctcacc actcggcttc
     4261 ctgctgaagg gggccggttt tgcccgcgcc gaggatgatg cccatgccgt gcagaacttc
     4321 atgctgtcgc gcgatgcggt gcgggcgctc gatggcgaac tgggcatcaa gcaggcctac
     4381 agcgcggccg atctggacct gctgagccgc tttcccgggc tcgactggga tgacagcttc
     4441 gagaactttc accgctactt ccagaagcag gtcaccatcc aactcgaccc cacctcatcg
     4501 atcagcatcc tgacggtgcg tgccttcagc gcgcagcagg ctgaggcgat caaccgccgg
     4561 ctgctggaac tgggcgagca actggtcaac cgcctgaacg agcgcggacg cgatgacctg
     4621 atccgctatg ccgccaagga ggtgagcgat ctcgaagcca aggccaaggg tgccgcgctg
     4681 gcgctggccc agtaccgcaa tgcccagggc gtgatcgacc cggagcggca atcgagcatc
     4741 cccctgcaac agatcgccaa actgcaagaa gagctgatcg ccaccaaggc gcagcgcctg
     4801 caactggagc gggtggccag agagaacccg caactgccac tgctgcaaca gcgcatccag
     4861 ctgctcgaac aggagatcga ggccgcatcg atgcgcgtgg cgggcggcga ccgctccctg
     4921 gccggcaagg cggcggagtt ccagcggctg gcactggaga aggagtttgc cgacaagatg
     4981 ctcgccagtg ccatgagcac cctggaactg gcccgcaacg aagcgcagcg ccagcagctc
     5041 tacctcgaac gggtcgccca gcccagcctg cccgaccagg cgctgga
//
LOCUS       DAORCQ010000132         4958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_12707_length_4958_cov_5.488272, whole genome shotgun sequence.
ACCESSION   DAORCQ010000132 DAORCQ010000000
VERSION     DAORCQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12707_length_4958_cov_5.488272"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PKI23_10650"
     CDS             complement(<1..762)
                     /locus_tag="PKI23_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37436.1"
                     /translation="MSIKIDRKIIGQKVIKEEDESTPAAAPPAPPAPAATEKRAADII
                     QMHESVQRPNILQGHTYKVKTPGHVSEHALYITINDILLNPGTEYELRRPFEIFINSK
                     NMDHFQWIVALTRIISAVFRKGGDVTFLVEELRSVFDPRGGYWKKGGKYVPSLVAEIG
                     DVIEQHLIRIGMLKAPELDAAQKQLLEEKRKEFALTPLDAQTDEAAEEGVARTDAGRA
                     GGTRDPLLHLLEHLRADRVHLDDHAEEERFVPGEER"
     gene            complement(796..2955)
                     /locus_tag="PKI23_10655"
     CDS             complement(796..2955)
                     /locus_tag="PKI23_10655"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNN37437.1"
                     /translation="MTSTRLSAVSNSLPAAMELPLQEASQDIWDKKYRLVQKSGAPVD
                     ADIDATYQRVARALAEVESSPEKVEEWYRRFLWALRQGCIPAGRIISNAGAQAYKPST
                     STINCTVSGTIGDSMDDILGKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSG
                     PLSFMDIYDKMCFTISSAGGRRGAQMATFDVRHPDVLDFIAAKREDGRLRQFNLSLLI
                     TDDFIQAVKQGRPWPLAFPIRSDEADDFPHIDQHPDILWLEWPVSDGMVVNAEGLVAC
                     RIYRTIPAQRLWDRIMSATYDYAEPGFVLIDKVNEMNNNWFCEKIRATNPCGEQPLPP
                     YGSCLLGSINLTKFVLDPFTERARFDWPRFREAVLVFTRMLDNVVEINGLPLPQQRHE
                     IMHKRRHGMGYLGLGSTLAMLRMTYGDAQSVTFTDEVSRELAITGWRAGLDLAREKGP
                     APIMDELFTVTAEMLRQRPEMAVDGHKVGDQLPGRVLHARYSRYMQRVASVEPELVAE
                     LASVGARFTHHSSIAPTGTISLSLANNASNGIEPSFAHQYSRNVIRAGKKSKEKVEVF
                     SFELLAYRALVNPDAMPYASDPAAQLPDYFVTTDDIPARSHVDIQAAAQRWIDSSISK
                     TINVPTDFAFDTFKEIYLYAYDQGLKGCTTFRFNPEAFQGVLVKEQDLERTRYQFTLD
                     DGSVVEVKGNDEIEYEGETHTAANLYDALKEGYYGKF"
     gene            complement(3168..>4958)
                     /locus_tag="PKI23_10660"
     CDS             complement(3168..>4958)
                     /locus_tag="PKI23_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNN37438.1"
                     /translation="EVTMALAWQVWLTTGLTLSTLLLLIFTRAPSDMVMMAAVLVLAM
                     SGILTPSEAFSGFSNSGLLTVAAMFVLAGGIRASGGVDLVVNQLLRTPKSERRAIGRL
                     MAPVMLASAFINNTPVVATMIPAVARWSKKIKVPVSSLMIPLSYASILGGTVTLIGTS
                     TNLVVNGQYQTLTGRPGFGLFDITPLGIAVALVGMSFMMLAAPRLLPNRQGTEQAFAN
                     KKQFTFEVAVAADGPLVGKSIIEAGLRHLQRIYLAEIERRGHILTAVTSEERLEGGDR
                     LVFVGETDAIIDVLRINGLVASEGAEPVIERNAPERRLVEVVISPECECIGQTIRDSQ
                     FRSRYGAVVLAVARGGRHIKGNLGSIELQTGDLLLLEARPSFIARQHALRDFLVINDL
                     NEERPNHDKAPISWAILLGVIGLASFELLDMLNAALLGAGLMILTRCCSASEARRSLD
                     LPVLITIGASLALGKGLEKSGAAEMLAQQLMTLASGDPWLLLALSYFTVMLLTELITN
                     NAAAAIMLPIVLAMCGPLGLDPVPFTVAVMFAASASFATPLGYQTNMMVYGPGGYQFS
                     DFLRMGAAMNLIVGVVTVLLIPLFWPLQAG"
BASE COUNT          866 a         1551 c         1644 g          897 t
ORIGIN      
        1 acgctcctca ccgggaacaa accgttcttc ctcggcgtga tcgtcgaggt gaacccgatc
       61 agcccgcaga tgctccagca gatgaagcag tggatctcgc gtcccgccgg ctcgaccggc
      121 atcggtccgg gcgacgccct cttcggcagc ttcgtcggtc tgggcatcga gcggggtcag
      181 ggcgaactcc ttgcgtttct cttcgagcag ctgcttctgg gcggcgtcga gttcgggcgc
      241 cttcagcatg ccgatgcgga tcagatgctg ttcgatcacg tcgccgatct cggccaccag
      301 cgaggggacg tacttgccgc ccttcttcca gtagccacca cgcgggtcga acaccgagcg
      361 cagctcctcg accaggaagg tgacgtcacc ccccttgcgg aacacggccg agatgatgcg
      421 ggtcagcgcg acgatccact gaaagtggtc catgttcttg gagttgatga agatctcgaa
      481 cggccggcgc agttcgtact cggtgccggg gttgagcagg atgtcgttga tggtgatgta
      541 gagggcatgt tcggagacat ggcccggcgt cttcaccttg taggtgtggc cttgcaggat
      601 gttgggccgc tgcaccgact catgcatctg gatgatgtcg gccgcgcgct tctcggtcgc
      661 tgccggggcc ggtggcgcgg gcggcgccgc ggccggggtg ctttcgtcct cttccttgat
      721 caccttctgg ccgatgatct ttctgtcgat cttgatgctc atggtctcac ctgggtgtgg
      781 ggttgccgct ggggttcaga acttgccgta gtagccctct ttgagcgcat cgtagaggtt
      841 ggcggcggtg tgggtttcac cttcgtattc gatctcgtcg ttgcccttca cttcgacgac
      901 gctgccatcg tcgagggtga actggtagcg ggtgcgctcc agatcctgct ccttgaccag
      961 cacgccctgg aaggcttcgg ggttgaagcg gaaggtggtg cagcccttca gcccctggtc
     1021 gtaggcgtac agatagatct ccttgaaggt gtcgaaggcg aagtcggtcg gcacgttgat
     1081 ggtcttggag atcgaggagt cgatccagcg ctgcgcggcg gcctggatgt cgacatggct
     1141 gcgggccggg atgtcgtcgg tggtgacgaa gtagtcgggc agttgcgcgg cggggtcgct
     1201 ggcatagggc atcgcgtccg ggttgaccag cgcacggtag gcgagcagtt cgaacgagaa
     1261 gacctcgacc ttctccttac tcttcttgcc ggcacggatg acgttgcgcg aatactggtg
     1321 ggcaaagctc ggctcgatgc cgttgctggc attgttggcc agcgacagcg agatggtgcc
     1381 ggtcggggcg atcgagctgt ggtgagtgaa gcgggcgccg acgctggcca gttcggccac
     1441 cagctcgggt tcgacgctgg caacgcgctg catgtagcgg ctgtagcggg catgcagcac
     1501 ccggcccggc agttggtcac ccaccttgtg gccatcgacg gccatctccg gccgctgccg
     1561 cagcatttcg gcggtgacgg tgaagagctc atccatgatc ggcgccggcc ccttctcgcg
     1621 ggccaggtcg agcccggcgc gccagccggt gatcgccagc tcgcggctca cctcgtcggt
     1681 gaaggtgacc gactgcgcat caccataggt catgcggagc atcgccagcg tcgagccgag
     1741 gccgaggtag cccatgccgt ggcgccgctt gtgcatgatc tcgtgacgct gctgcggcag
     1801 cggcaggccg ttgatttcga cgacattgtc gagcatgcgg gtgaagacca gcaccgcctc
     1861 gcggaaccgt ggccagtcga aacgggcccg ctcggtgaag gggtcgagca cgaacttggt
     1921 caggttgatc gagcccagca ggcaggagcc atagggcggc agcggctgct cgccacaggg
     1981 gttggtggcg cgaatctttt cgcagaacca gttgttgttc atctcgttga ccttgtcgat
     2041 caacacgaag cccggctcgg cgtagtcgta ggtggccgac atgatgcggt cccacagccg
     2101 ctgtgccgga atcgtccggt agatccggca ggccaccagc ccttcggcat tgaccaccat
     2161 gccatcactg accggccatt cgagccagag gatgtcggga tgctggtcga tgtgcggaaa
     2221 atcgtcggcc tcgtcggagc ggatcggaaa ggccagcggc caggggcgcc cttgcttcac
     2281 cgcctggatg aagtcgtcgg tgatcagcag cgacagattg aactgccgca gccggccatc
     2341 ctcgcgcttg gcggcgatga aatcgagcac gtcgggatgg cgcacgtcga aggtggccat
     2401 ctgcgcgccg cgccggccgc cggccgagga gatggtgaaa cacatcttgt cgtagatgtc
     2461 catgaacgac agcggccccg aggtgtaggc tccggcgccc gagacatagg cgccgcgcgg
     2521 gcgcagtgtc gaaaattcgt agccgatgcc gcagccggcc ttgagggtca gaccggcctc
     2581 gtgcaccttg ccgaggatgt catccatcga gtcgccgatg gtgccggaga ccgtgcagtt
     2641 gatggtcgag gtggagggtt tgtaggcctg ggcaccggcg ttggagatga tgcgcccggc
     2701 cggaatgcag ccctgacgca gcgcccacag aaagcggcgg taccactcct cgaccttttc
     2761 cgggctgctc tcgacctcgg ccagcgcgcg cgccacccgc tggtaggtgg catcgatgtc
     2821 ggcatcgacc ggcgcgccac tcttctgcac caggcggtat ttcttgtccc agatatcctg
     2881 cgaggcctcc tgcagcggca gctccatggc ggccgggagg ctgttgctga cggccgacaa
     2941 ccgggttgaa gtcatgatcg tgcttcctgt gcgggtgttt ttgttgtgtg ttggactgcg
     3001 cacggaatac tacatcatgt gccttaaaaa taaaaacaca ccagatatag tgtaaagagc
     3061 aaaaaattct tgcgaaagga actttgccgg cagatcagcc ggttagcaga gatcggctgc
     3121 gctcacccag ctcgaccggg tgagcgaaac cgcggagcgg atccggctca gccggcctgc
     3181 agtggccaga acagcggaat cagcagcacg gtcaccacgc cgacgatcag gttcatcgcc
     3241 gcgcccatgc gcagaaagtc ggagaactga taaccgcccg ggccatagac catcatgttg
     3301 gtctgatagc cgagcggcgt cgcgaagctg gccgatgcag cgaacatcac cgccaccgtg
     3361 aaggggaccg gatcgagccc gagcggcccg cacatcgcca gcacgatcgg cagcatgatc
     3421 gccgcggcgg cattgttggt gatcagctcg gtcagcagca tcaccgtgaa gtaactgagc
     3481 gccagcagca gccaggggtc gccgctggcc agcgtcatca gttgttgggc cagcatttcg
     3541 gcggcgccgc tcttctccag tcccttgccg agcgccaggc tggcgccaat ggtgatcagc
     3601 accggcagat cgaggctgcg ccgcgcctcg ctcgccgagc agcagcgggt caggatcatc
     3661 agcccggcgc ccagcagcgc ggcattgagc atgtcgagca gctcgaaact ggcgaggcca
     3721 atcaccccca gcaggatggc ccagctgatc ggcgccttgt catggttggg ccgctcctcg
     3781 ttcaggtcgt tgatcaccag aaagtcgcgc agcgcatgct ggcgggcgat gaaagagggg
     3841 cgtgcctcca gcagcagcag atcaccggtc tgcaactcga tcgagccgag gttgcccttg
     3901 atgtggcgcc cgccgcgtgc caccgccagc accaccgcgc cgtaacggct gcgaaactgg
     3961 ctgtcgcgga tggtctgccc gatgcattcg cactcgggcg agatcaccac ctcgaccagc
     4021 cgccgctccg gggcattgcg ctcgatcacc ggctcggcgc cttccgaggc gaccagcccg
     4081 ttgatgcgca gcacatcgat gatcgcatcg gtctcgccga cgaagaccag ccggtcaccg
     4141 ccttcgagcc gctcctcgct ggtcaccgcg gtcaggatgt ggccacggcg ctcgatctcc
     4201 gccagataga tgcgctgcag gtggcgcagt ccggcctcga tgatgctctt gccgaccagc
     4261 gggccatcgg cggccaccgc cacttcgaag gtgaactgct tcttgttggc gaatgcctgc
     4321 tcggtcccct gccggttggg cagcagccgc ggcgcggcca gcatcatgaa cgacatgccg
     4381 accagcgcca cggcaatgcc gagcggggtg atgtcgaaca ggccgaagcc ggggcgaccg
     4441 gtcagcgtct ggtactgacc attgaccacc aggttggtgc tggtgccgat cagcgtaacc
     4501 gtaccgccca gaatcgaggc gtagctgagc gggatcatca gactcgacac cggcaccttg
     4561 atcttcttgg accagcgcgc cacggcggga atcatcgtcg ccaccaccgg cgtgttgttg
     4621 atgaaggcgc tggccagcat caccggcgcc atcagccgac cgatggcgcg ccgctcgctc
     4681 ttcggcgtac gcagcagctg gttgaccacc agatcgacac cgccggaagc gcggatgccc
     4741 ccggccagca cgaacatcgc cgccaccgtc agcagaccac tgttgctgaa gccggagaag
     4801 gcctcactgg gggtcaggat gccgctcatc gccagcacca gcacggccgc catcatcacc
     4861 atgtccgaag gagcgcgagt gaagatcagc agcaacagcg tcgaaagggt gagtccagtg
     4921 gtcagccaga cctgccatgc cagtgccatc gtcacctc
//
LOCUS       DAORCQ010000133         4935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_12796_length_4935_cov_5.545902, whole genome shotgun sequence.
ACCESSION   DAORCQ010000133 DAORCQ010000000
VERSION     DAORCQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12796_length_4935_cov_5.545902"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..922)
                     /locus_tag="PKI23_10665"
     CDS             complement(257..922)
                     /locus_tag="PKI23_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNN37439.1"
                     /translation="MSHPPHWQHGGATPWPLGKILCVGRNYAEHARELGNPQPAEPIL
                     FIKPATAAVDFKRPWSIPTDRGACHVETELALLIGQPLRRAAAEQTMAAIAGYGIGLD
                     LTLRELQNRLKQAGHPWEVAKAFDGSCPLTPFIPAAELGDPTRLCFTLELNGRPQQRG
                     DVTQMLFSIPQLLQTMSHHFTLLPGDVVLTGTPAGVTALQPGDQLRLTLAQYQWQGEV
                     AAD"
     gene            complement(969..2363)
                     /locus_tag="PKI23_10670"
     CDS             complement(969..2363)
                     /locus_tag="PKI23_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNN37440.1"
                     /translation="MNQFASSPFLQSLHTLLGSALLTDPVECHPYGGDWSRQHEPAPL
                     AVALPRSVDEVVALVKLAREFGVALVPSGGRTGLSGAAVAAHGELVVAFDRMQRILDF
                     NPIDRTVTVEAGLVTAQLQQFAQQQGLFYPVDFASAGSSRIGGNIATNAGGIKVIRYG
                     LTRDWVAGLKVVTGSGELLELNQGLIKNATGYDLRHLFIGSEGTLGLIVEATLRLTLP
                     PRPLKVAVLGVPGLSAIMEVLAAFRSRVALTAFEFFSEAALTHVVQRTGLPRPFEEAT
                     PYYCLVELECEPHEEAQLLACFEHCLESGWVSDGVISQSEAQLRNLWRLREDISETIA
                     PFTPYKNDLAVRISAIPAFLQAVDALVQQHYPEFEVIWFGHIGDGNLHLNILKPANWE
                     KSAFFAECAKVSELIFAEVKRAGGSISAEHGVGLLKKPYLGYSRAPAEIALMRGIKAA
                     FDPDGILNPGKIFD"
     gene            complement(2420..2791)
                     /locus_tag="PKI23_10675"
     CDS             complement(2420..2791)
                     /locus_tag="PKI23_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012277143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNN37441.1"
                     /translation="MLTGTLHPLWLTVLLLSLSNVFMTFAWYAHLKALSHKPWIIAAL
                     VSWGIALFEYLLQVPANRIGHSVLSVAQLKILQEAITLTVFVPFALFYLKEPLKLDYL
                     WAALCVLGAVYFIFRSRWNAI"
     gene            complement(2799..3236)
                     /locus_tag="PKI23_10680"
     CDS             complement(2799..3236)
                     /locus_tag="PKI23_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HNN37442.1"
                     /translation="MRIWSLHPRYLDAKGLVALWREALLAQAVLAGQTRGYQRHPQLL
                     RFSRSANPDHAIASYLRAVHAEATHRGYRFDAAKIGPGEVTTPMPVTQGQLLYEWSHL
                     VRKLHTRAPAWLDGLDSMGQPDPHPLFRVVAGEIEPWEVITAQ"
     gene            complement(3281..4810)
                     /locus_tag="PKI23_10685"
     CDS             complement(3281..4810)
                     /locus_tag="PKI23_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HNN37443.1"
                     /translation="MPIVETRHGRLRGSLHQGIAAFKGIPYAAPPVGALRWRPPADPL
                     RWSGVRDAIDYGPWAPQRSSTLDGVMGAEQAAQSEDCLTLNLWSPGLDQVRRPVMVWI
                     HGGSFARGSGGQGLFEGHALSRHGDLVVVTLNYRLGVFGFSHLATATAGAVPATGNEG
                     LLDQIKALQWVQDNIEHFGGDPHNVTLFGESAGAMAIGALLTSPAARGLFHKAILQSG
                     ACHSFATLASAPLLGEALLKATGLDADRLCMASTAELLLAQQSIEHGQVEGYPLARIG
                     GLPFRPVVDGTVLPVRPYAAIEAEVAAGIPLMVGTTADEWRLFGALQPAITGLSEANL
                     FKRLGYFIDHAQLPALVDSYRQTLVARGIEPTPPELFMAIQTDRVFRIPALRLLERQQ
                     PHEHRLYSYLFDWKSPAAGGALGACHAIELAYVFGTHTKPGAAGFFGTGAAADALARQ
                     CMSAWSAFARNGDPGWPAYDSEQRATMILGERCRSEQAPFDRERAAWESIDDLALGSL
                     A"
BASE COUNT          827 a         1551 c         1739 g          818 t
ORIGIN      
        1 cgaagctgct ggatgaggta cgcgcccaca tccggcggtt gaactacagc atccgggacc
       61 gaatcgggga cggactgcag aatcggggac agaccacggt ttcccattct tgatcaaggc
      121 gctttcccaa actgcgatat tgaatgaacc atggtctgtc gccatttccg cgctccgatc
      181 tctctttttc cggtgcgatc atcggccgca cgaggtgagc ggcaagaaaa gggcgcgcag
      241 ccaaggataa caagcctcag tctgccgcca cctcgccctg ccactggtac tgggccaagg
      301 tcaaccgtaa ttgatcaccc ggttgcagcg cagtgacgcc ggccggggtg ccggtcagca
      361 ccacgtcgcc gggcagcagg gtgaagtggt ggctcatcgt ctgcagcagt tgcggaatcg
      421 aaaacagcat ctgtgtcacg tcaccgcgct gctgcggtcg gccattgagt tcgagcgtga
      481 agcacagtcg tgtcgggtcg cccagttcgg cggccgggat gaagggggtg agcgggcaac
      541 tgccgtcgaa ggccttggcc acctcccagg ggtggccggc ctgcttcagg cggttctgca
      601 gctcgcgcag cgtcaggtcg agcccgatgc cgtagccggc gatcgccgcc atggtctgct
      661 ccgcggctgc ccggcgcagc ggctgaccga tcagcagcgc cagttcggtt tcgacatggc
      721 aggcgccccg gtcggtcgga atcgaccagg ggcgtttgaa gtctacggcc gccgtggcgg
      781 gtttgatgaa gaggatcggt tccgccggct gcggattgcc cagctcgcgg gcatgttcgg
      841 catagttgcg gccgacgcag aggatcttgc ccagcggcca gggtgtcgcg ccgccatgct
      901 gccaatgggg tgggtgactc atcggtctgc tccgtggtgc tgcgggggac ctgccgggcg
      961 gccccaggtc aatcgaagat cttgccgggg ttgagaatgc catcggggtc gaaggccgcc
     1021 ttgatgccgc gcatcagtgc gatctcggcc ggggcgcggc tgtagccgag gtagggcttc
     1081 ttcagcaggc ccacgccatg ttcggcggag atgctgccgc cggcccgctt cacctcggcg
     1141 aagatcagtt cgctgacctt ggcgcattcg gcgaagaagg cgctcttctc ccagttggcc
     1201 ggtttgagga tgttgaggtg gaggttgcca tcgccgatgt ggccgaacca gatcacctcg
     1261 aattcgggat agtgctgctg taccagtgca tcgaccgcct gcagaaaggc cgggatggcc
     1321 gagatgcgca ccgccaggtc gttcttgtag ggggtgaagg gggcgatggt ctcggagatg
     1381 tcctcgcgca gccgccacag gttgcgcagt tgcgcctcgc tctggctgat cacgccgtcg
     1441 ctgacccagc ctgattcgag gcagtgctcg aagcaggcca gcagttgcgc ctcttcatgt
     1501 ggctcgcact ccagttccac cagacagtag tagggggtgg cctcttcgaa ggggcgtggc
     1561 aggccggtgc gctgcaccac gtgggtgagt gcggcctcgg agaagaactc gaaggcggtc
     1621 agggccaccc ggctgcgaaa ggctgcgagc acctccatga tggcggagag ccccggcacg
     1681 ccgagcaccg ccaccttgag cggtcgtggc ggcagcgtca gccgcagcgt ggcctcgacg
     1741 atcagcccga gggtgccttc ggagccgatg aagaggtggc gcaggtcgta gccggtggca
     1801 ttcttgatca gcccctggtt cagctcgagc agttcaccgc tgccggtcac caccttcagt
     1861 ccggccaccc agtcgcgggt caggccgtag cggatcacct tgatgccgcc ggcattggtg
     1921 gcgatgttgc cgccgatgcg gctggagccg gccgaggcga aatcgaccgg atagaacagc
     1981 ccctgctgct gcgcgaactg ctgcaactgc gcggtcacca gcccggcctc gacggtgacg
     2041 gtgcggtcga tcgggttgaa atcgaggatg cgctgcatcc ggtcgaaagc gaccaccagc
     2101 tcgccgtggg cggccaccgc cgcgccactg aggccggtgc gtccgcccga aggcaccagc
     2161 gccacgccga actcgcgcgc cagcttcacc agcgccacca cttcgtcgac gctgcgcggc
     2221 agcgccaccg ccagtggcgc gggctcatgc tgccggctcc agtcgccgcc atagggatga
     2281 cactcgaccg gatcggtcag cagagcgctg ccgagcaggg tgtggaggct ttgcagaaaa
     2341 ggagaggagg cgaattggtt catcggcggt gacttcggag tggatggcgg gtcatgcggc
     2401 tgtggcgcat gacagcatat caaatggcgt tccagcggct gcggaaaatg aagtagaccg
     2461 ctcccagcac acagagcgcg gcccacaggt agtcgagctt cagcggctcc ttcaggtaga
     2521 acagcgcaaa ggggacgaag accgtcaggg tgatggcctc ttgcagaatc ttcaattggg
     2581 cgaccgacag cacgctgtgg ccgatgcggt tggcgggcac ctgcagcagg tattcgaaga
     2641 gggcgatgcc ccagctgacg agcgcggcga tgatccaggg cttgtggctc aacgccttca
     2701 gatgggcata ccaggcgaag gtcatgaaga cattgctgag cgacagcagc agcacggtca
     2761 gccagagggg atggagggtg ccggtcagca tcgggatctc actgtgccgt gatcacttcc
     2821 cagggttcga tctcgccggc caccaccctg aacagcgggt ggggatcggg ttgccccatg
     2881 ctgtcgagcc cgtcgagcca ggctggtgcg cgtgtgtgca gcttgcggac gaggtggctc
     2941 cactcgtaca gcagttgtcc ctgcgtcacc ggcatgggcg tcgtcacctc acccggaccg
     3001 atcttggcgg catcgaagcg gtaaccgcgg tgggtcgctt cggcatggac tgcccgcagg
     3061 tagctggcga tggcatgatc cggattggca ctgcgcgaga agcgcagcag ttgcgggtgg
     3121 cgttggtagc cgcgggtctg cccggccagc acggcctgtg ccagcagggc ctcgcgccag
     3181 agggcgacca ggcctttggc gtcgaggtaa cggggatgaa gagaccagat gcgcatgggg
     3241 tctcatgctc cagcgggtgt gaattgctgg gtcgaaccga tcatgccagc gaccccagag
     3301 ccaggtcgtc gatcgactcc caggccgcgc gttcgcggtc gaacggcgcc tgttcgctgc
     3361 gacagcgttc gccgaggatc atcgtggcac gctgctcgga atcataggca ggccagcccg
     3421 gatcgccgtt gcgggcgaac gcgctccagg cgctcatgca ctgccgcgcc agcgcatccg
     3481 ccgccgcgcc ggtgccaaag aagccggcgg cgcccggctt ggtgtgggtg ccgaagacat
     3541 aggccagctc gatggcatgg caggcgccca gtgcgccgcc ggcggccggc gatttccagt
     3601 cgaacaggta gctgtagaga cggtgctcat gcggctgctg ccgctccagc agccgcagtg
     3661 cagggatgcg aaagacgcgg tcggtctgaa tcgccatgaa cagctcgggc ggggtgggtt
     3721 cgatgccgcg tgcaaccagt gtctgccggt agctgtcgac cagtgcgggg agttgggcgt
     3781 ggtcgatgaa gtagcccagg cgtttgaaca ggttcgcctc gctgagaccg gtgatggccg
     3841 gttgcagcgc cccgaacagc cgccactcat cggcggtggt gccgaccatc agcggaatgc
     3901 cggccgcgac ctccgcctcg atcgctgcat agggtctgac cggcagcacc gtgccgtcga
     3961 ccaccggccg aaatggcaga ccgccgatgc gtgccagcgg gtagccctcg acctgcccat
     4021 gctcgatcga ctgctgcgcc agcagcagct ctgccgtgct ggccatgcac agccggtcgg
     4081 cgtcgaggcc ggtggccttc agcagcgcct cgcccagcag cggcgccgag gccagggtgg
     4141 caaagctgtg gcaggcgccc gattgcagga tcgccttgtg gaacaggccg cgtgccgccg
     4201 gcgacgtcag cagggcgccg atcgccatcg cgccggccga ctcgccgaac agcgtgacat
     4261 tgtgcggatc gccgccaaag tgttcgatgt tgtcctgcac ccactgcagc gccttgattt
     4321 gatcgagcag cccctcgttg ccggtggcgg gaactgcgcc ggcggtcgcc gtggccagat
     4381 ggctgaagcc gaagaccccc aggcggtagt tgagcgtgac gacgaccagg tcgccgtggc
     4441 ggctcagcgc atgaccctcg aacaacccct gcccacccga gccacgggcg aagctgccgc
     4501 catgaatcca caccatcacc ggccgtcgga cttggtcgag gccggggctc cagagattca
     4561 gcgtcagaca atcctccgac tgcgccgcct gctctgcgcc catcacgcca tcgagggtgg
     4621 agctgcgctg cggtgcccag gggccgtagt cgatggcgtc gcgcacgccg ctccagcgca
     4681 ggggatcggc cggcggcctc cagcgcagtg caccgaccgg tggtgcggcg taggggatgc
     4741 ccttgaatgc cgcgatgccc tgatgcaggc tgccacgcag acggccgtgg cgggtttcga
     4801 cgatcggcat ggtggactcc tgggcagtgg cggggagatt catccgtgct ggaacggccg
     4861 gttctcgatc ggcgtgcaag cggtgcaatg gtagaccgca ggggtggtcg tggtgaaccc
     4921 cgcagccgcg gcccc
//
LOCUS       DAORCQ010000134         4884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_13003_length_4884_cov_6.158625, whole genome shotgun sequence.
ACCESSION   DAORCQ010000134 DAORCQ010000000
VERSION     DAORCQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13003_length_4884_cov_6.158625"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(488..676)
                     /locus_tag="PKI23_10690"
                     /pseudo
     CDS             complement(488..676)
                     /locus_tag="PKI23_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
     gene            complement(895..1584)
                     /gene="rpiA"
                     /locus_tag="PKI23_10695"
     CDS             complement(895..1584)
                     /gene="rpiA"
                     /locus_tag="PKI23_10695"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNN37444.1"
                     /translation="MDQSHLKLAAAQAALDHLRPRLDDRSVVGIGTGSTANHFIDLLA
                     GIRSQFDGAVASSQASEARLRQHGIPVYDLNGVAELQFYVDGADEINPRLEMIKGGGG
                     ALTREKIVAAVAREFICIADQSKYVDLLGGYPLPIEVIPMARSHVARQLVKLGGDPVY
                     REGVVTDNGNIILDLHNLRIDVPREMEQRINNITGVVCNGLFAQRPADLLLLATDEGV
                     KRYRASDAVVR"
     gene            1762..1998
                     /gene="rpmB"
                     /locus_tag="PKI23_10700"
     CDS             1762..1998
                     /gene="rpmB"
                     /locus_tag="PKI23_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNN37445.1"
                     /translation="MSRVCQITGKRPMTGNNVSHANNKTRRRFEPNLHYHRFWVESQK
                     RFVKLRVSSKGMRIIDKVGIDQILADLTARGEKF"
     gene            2030..2185
                     /gene="rpmG"
                     /locus_tag="PKI23_10705"
     CDS             2030..2185
                     /gene="rpmG"
                     /locus_tag="PKI23_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNN37446.1"
                     /translation="MRDKIKLLSTAGTGHFYTTDKNKRTMPEKMEIKKFDPIARKHVI
                     YKEAKIK"
     gene            complement(2278..3051)
                     /gene="hisF"
                     /locus_tag="PKI23_10710"
     CDS             complement(2278..3051)
                     /gene="hisF"
                     /locus_tag="PKI23_10710"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNN37447.1"
                     /translation="MGLAKRIIPCLDVDRGRVVKGVKFVEIRDAGDPVEIARRYDQQG
                     ADELTFLDITASHEDRATMVQVVEAIAGEVFIPLTVGGGIRAIEDVRRMLNAGADKVA
                     INTAAVSNPHFVAEAAERFGAQCIVVAIDAKRVSGDDEPARWEIFTHGGRKPTGIDAI
                     EWGVRMQQLGAGEILLTSMDRDGTRIGFDLGLTRAMSEAVPIPIIASGGVGTLQHLVD
                     GVLIGQADAVLAASIFHFGEFTVPDAKRYMAAHGIEMRL"
     gene            complement(3057..3797)
                     /gene="hisA"
                     /locus_tag="PKI23_10715"
     CDS             complement(3057..3797)
                     /gene="hisA"
                     /locus_tag="PKI23_10715"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNN37448.1"
                     /translation="MTFLVIPAIDLKDGECVRLRQGRMEESTVFSQDPLAMAAHWVAA
                     GARRLHLVDLNGAFAGNPVNGEIITAISRRFPQLPIQIGGGIRSLETAERYLAAGVSH
                     LIIGTQAVKQPSFVAEACRAFPGHIMVGLDARGGRVATEGWAEQSGIEAVQLARQFAD
                     CGVSALIYTDIGRDGMMQGVNIEATVELARAAAIPVIASGGVTSIDDIRALKKVASSG
                     IIGAITGRAIYEGSLDLAAAQRLADSEE"
     gene            complement(3794..4459)
                     /gene="hisH"
                     /locus_tag="PKI23_10720"
     CDS             complement(3794..4459)
                     /gene="hisH"
                     /locus_tag="PKI23_10720"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNN37449.1"
                     /translation="MAQRIAIIDYGMGNLHSAAKALEHVAPAAKVEVTADRRTILAAD
                     RVVLPGVGAIRDCIGEMRRLGIDQLLPEVIERKPLLAICVGLQALLESSEENGGVTCL
                     GLLPGRVRHFGAPLREADGSRLKVPHMGWSRVWQQPHPLWSGIADGSRFYFVHSYYVE
                     AARADQVVGQCHYGVDFAAAMASPNLFAVQFHPEKSQQAGLALLRNFSRWDGAAVVEG
                     VTS"
     gene            complement(4468..>4884)
                     /locus_tag="PKI23_10725"
     CDS             complement(4468..>4884)
                     /locus_tag="PKI23_10725"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HNN37450.1"
                     /translation="DLQIDAHHTVEDIGITLGQAFRQAAGDKQGIQRYGHAYVPLDEA
                     LSRVVIDLSGRPGLQMAVPYRRATVGGFDVDLFEEFFRGFVNHAMVTLHIDTLRGENT
                     HHQIETVFKAFGRALRMAVARDPAMAGSTPSTKGSL"
BASE COUNT          928 a         1604 c         1490 g          862 t
ORIGIN      
        1 aaccaaacag tgatgcataa cccggcagtc cacacggacg ctgcgcggta aaaccgcgca
       61 gcgccggtga cttccacgtt atgcctcatc aggagaagct ttcatgcgac gcacaatcgt
      121 tggcgcaata ctgctctgcc tttcgctcaa tgcttcagct gcggatgatt ccgtgttcgg
      181 aacgcattgg gcgaaatttc agcctatcca agtggagggt gatgtccagg gatgtcagct
      241 aacgttcttg acggtaactg cggatcgcgt ttatctcgat ggcaatcaag tcgctgttaa
      301 tggcagcata agaattggta agaattgggg acagaccacg gtttcttcgg tttcctgccg
      361 ctcagcgtac cacagtagta gggtgggcaa aggccgccag gccgtgccca cgcgaaacgg
      421 ggccagaacc agcggggtaa ttggggacag accacggttt cttcggtttc ctgccgctca
      481 gcgtgcagac cacgccggtg atgttgttga tccgctgctc catctcgcgt ggtacgtcga
      541 tgcgcaggtt gtgcaggtcg agaatgatgt tgccattgtc ggtcaccacc ccctcgcgat
      601 agaccggatc cccgcccagc ttgaccagtt ggcgggcaac atgactgcgc gccatcggga
      661 tcacctcgat cggcagcacg gaatcgggga cacagcacgg aatcggggac agaccacggt
      721 ttcttcggtt tccctgccgc tcagcgtacc acagtagtag ggtgggcaaa ggccaccagg
      781 ccgtgcccac acgaaacggg gccagaacca gcgtgggcac gctgcgcttt gcccacccta
      841 cttcttctgc ttcggggata gctcaaaacc acggtttctt ctgtttccgg acgctcagcg
      901 caccacggca tcgctagccc gataacgctt caccccttca tcggtggcca gcagcaacag
      961 gtcggccggc cgctgggcga agaggccatt gcagaccacc ccggtgatgt tgttgatccg
     1021 ctgctccatc tcgcgtggta cgtcgatgcg caggttgtgc aggtcgagaa tgatgttgcc
     1081 attgtcggtc accaccccct cgcgatagac cggatccccg cccagcttga ccagttggcg
     1141 ggcaacatga ctgcgcgcca tcgggatcac ctcgatcggc agcgggtagc cgccgagcag
     1201 atcgacatac ttgctctggt cggcaatgca gatgaactcg cgggccaccg ctgcgacgat
     1261 cttctcccgg gtcagggcgc caccaccgcc cttgatcatc tccagccggg ggttgatctc
     1321 atcggcgccg tcgacgtaga actgcaactc cgccacgcca ttcaaatcgt agaccgggat
     1381 gccatgctgg cgcagccgcg cctcgctggc ctgtgagctg gccaccgcgc catcgaactg
     1441 gctcctgatg ccggccagca ggtcgatgaa gtggttggcg gtcgaaccgg tgccgatgcc
     1501 caccacgctg cggtcatcga gccgaggacg cagatggtcg agcgctgcct gggcggcagc
     1561 cagtttcaag tggctctgat ccatgcggcc tctcctgttg cttctgggaa tgggccgcca
     1621 ttatagcgcc tgcaaggtcc gacgccggcc gccgctgccg gcaggatgcg cgcctgacac
     1681 cacatgcctt ttgcttggca gtgcgctttc tggtataaag gtcacccttt tttctggatg
     1741 cggcccaggg gcgtaacgaa gatgtcgaga gtctgtcaaa tcaccgggaa acggccaatg
     1801 actggcaaca atgtctccca tgccaacaac aaaacccggc gccggttcga gccgaatctg
     1861 cactaccacc gcttctgggt cgagtcgcag aagcgcttcg tcaagctgcg ggtctcgtcg
     1921 aagggcatgc gcatcatcga caaggtcggc atcgatcaga tactggccga cctgaccgcc
     1981 cgtggcgaga agttctgagc ccagacccac ctgaatcagg agagatccga tgcgtgacaa
     2041 gatcaaactg ctctcaaccg ccggcaccgg ccacttctac accaccgaca aaaacaagcg
     2101 gaccatgccc gagaagatgg agatcaagaa gttcgatccc atcgcccgca agcatgtgat
     2161 ctacaaggaa gccaagatca agtgatctga tgcggccagc tcaccctggc catcggaccg
     2221 gcaaacaaaa agcccgcctg cgacagccgg cgggcttttt gtttgcttgc ttgttggcta
     2281 cagtcgcatc tcgatgccat gcgccgccat gtagcgcttg gcatcgggaa cggtgaactc
     2341 gccaaagtgg aagatgctgg cggccagcac ggcatcggcc tggccgatca gcacaccatc
     2401 gaccaggtgt tgcaacgtgc caaccccgcc cgaggcgatg atcggaatcg gcaccgcctc
     2461 gctcatcgcg cgggtcaatc caaggtcgaa gccgatgcgg gtaccatcgc ggtccatgct
     2521 ggtcagcaga atctcaccgg caccgagctg ctgcatccgc acgccccact cgatggcgtc
     2581 gatgccggtg ggcttgcgtc cgccatgggt gaagatctcc cagcgcgccg gctcatcgtc
     2641 accactgacc cgcttggcgt cgatggcgac cacgatgcac tgcgcgccaa agcgttcggc
     2701 cgcctcggcg acgaagtgcg gattggagac cgccgcggtg ttgatcgcca ccttgtcggc
     2761 accggcgttg agcatgcgcc ggacatcttc gatggcgcgg atgccgccgc cgacggtgag
     2821 cggaatgaag acctccccgg caatcgcctc gaccacctgc accatggtgg cacggtcttc
     2881 atggctggcg gtgatgtcga gaaaggtcag ctcgtccgcc ccctgctggt catagcggcg
     2941 ggcaatctcg accggatcgc cggcatcgcg aatctcgacg aacttgaccc ccttgaccac
     3001 ccggccacgg tcgacgtcga gacagggaat gatccgtttg gcgagaccca tgtcggtcac
     3061 tcctcgctgt cggccaaccg ctgcgccgcg gccagatcga ggctgccctc gtagatcgcc
     3121 cggccggtga tcgcgccgat gatcccgctg ctggccacct ttttgagcgc acgaatgtcg
     3181 tcgatcgagg tgacaccgcc ggaggcgatc accggaatcg cggcagcccg cgccagttcg
     3241 acggtggcct cgatgttgac cccctgcatc atgccgtcac ggccgatgtc ggtgtagatc
     3301 agcgcgctga caccgcagtc ggcgaactgt cgcgccagtt gcaccgcctc gatgcccgac
     3361 tgctcggccc agccttcggt cgccaccctg ccgccccggg catcgagccc aaccatgatg
     3421 tgccccggaa aggcacggca ggcctcggca acgaagctcg gctgcttgac cgcctgggtg
     3481 ccgatgatca aatgactgac cccggccgcc agatagcgct cggcggtctc caggctgcgg
     3541 atgccaccgc cgatctggat cggcagttgc ggaaagcggc gcgagatcgc cgtgatgatc
     3601 tcgccattga ccggattgcc ggcaaaggcg ccattgaggt cgaccagatg cagccgccgg
     3661 gcgccggccg ccacccagtg ggccgccatc gccagcgggt cctgcgagaa gaccgtgctc
     3721 tcctccatgc gtccctgccg caggcgaaca cattcgccat ccttcagatc gatcgcggga
     3781 atgaccagaa aggtcatgat gtgactcctt caacaacggc ggcgccatcc cagcggctga
     3841 agttgcgcag cagggccagc ccggcctgct ggctcttctc gggatgaaac tgcaccgcaa
     3901 agaggttggg gctggccatg gcggcggcaa aatcgacccc atagtggcac tggccgacga
     3961 cctgatcggc acgcgccgcc tcgacatagt agctgtggac gaaatagaaa cggctgccat
     4021 cggcaatgcc gctccagagt ggatggggct gttgccatac ccggctccag cccatgtgcg
     4081 gcaccttcag ccggctgcca tcggcctcgc gcagcggcgc accgaagtgg cggacccgcc
     4141 ccggcagcag tccgaggcag gtgacaccgc cattctcctc actcgactcg agcagtgcct
     4201 gcagaccgac gcagatcgcc agcagcggct tgcgctcgat cacctccggc aacaactggt
     4261 cgatgccaag acggcgcatc tcgccgatgc agtcgcggat ggcccccacc cccggcagca
     4321 ccacccgatc cgccgccaga atggtgcgtc gatcggccgt gacttcgacc ttggcagcgg
     4381 gcgcgacatg ctcgagcgcc ttggcggccg agtgcagatt gcccatgcca tagtcgatga
     4441 tggcaatacg ctgagccatg ccgcgcctca aaggctgccc ttggtcgatg gcgtgctgcc
     4501 ggccatggcc ggatcgcggg cgaccgccat gcgcagcgcg cggccaaagg ccttgaagac
     4561 ggtctcgatc tggtggtggg tgttctcgcc gcgcagggtg tcgatgtgca gggtcaccat
     4621 tgcatggttg acgaagccac gaaagaactc ctcgaacagg tcgacatcga agccgccgac
     4681 cgtcgcccgc cggtagggca ccgccatctg caaaccgggc cgaccggaga gatcgatcac
     4741 cacccgcgac agcgcctcgt cgagcggtac gtaggcatgg ccatagcgct ggatgccctg
     4801 cttgtcgccc gctgcctgac gaaacgcctg gccgagggtg atgccgatgt cctccacggt
     4861 gtggtgggca tcgatctgca gatc
//
LOCUS       DAORCQ010000135         4872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_13054_length_4872_cov_6.233133, whole genome shotgun sequence.
ACCESSION   DAORCQ010000135 DAORCQ010000000
VERSION     DAORCQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13054_length_4872_cov_6.233133"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /locus_tag="PKI23_10730"
     CDS             complement(<1..2086)
                     /locus_tag="PKI23_10730"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNN37451.1"
                     /translation="MTLRMRFSLLPSLFMLFHLLACGVTVQAEPLKMNLSHQKFILDN
                     GLTVIVHEDRKAPIVAVNLWYHVGSKDEKPGKTGFAHLFEHLMFNGSEHHDDEYFKPF
                     ERVGATEMNGTTNNDRTNYFQNVPTSALDLALWMESDRMGHLLGAITQAKLDEQRGVV
                     QNEKRQGENQPYGRVFEAITKACYPAGHPYSWEVIGSMTDLNAATLDDVHQWFKDYYG
                     PSNAVLVLAGDIDLETAKQKVQHYFGDIAPGQPITRPKAWTAQRTGHQREVMQDRVPQ
                     PRLHQVWNVPELRDQNAPLFDLLGQLLAGGKNSRLYQRLVYRDQIATEVSAAYWGREL
                     SGLFVITADVKKGHTLAEVEKAIDQELTTLLTQGPGQSELERVRAAYTAQFVREAERV
                     GGTGGKSDLLASGEVFFDNPTAMVDIWLKQINAATPIALRDLSRKWLSDGRYTLEVEP
                     FPTLEAAKEGADRSQLPAVDEPPQLKIPELERATLKNGLKVVLAQRHEVPLVQLTLQF
                     DAGHSTDQGGKPGTQSFAMEMLTEGSSQYDALALADRLERLGATLTTSSTIDTSSLHL
                     STLKSNLTSSLGLFADVLLNPAFKSSEIERKRALWLARIEQEEASPVSLAMRLMPPLL
                     YGEGHPYALPFTGSGTVAGVSSLKRDDLVNFHRTWFQPGRATLIVVGDTTLKELVPLL
                     EQQLGQWRDRGAK"
     gene            complement(2194..3114)
                     /gene="xerD"
                     /locus_tag="PKI23_10735"
     CDS             complement(2194..3114)
                     /gene="xerD"
                     /locus_tag="PKI23_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNN37452.1"
                     /translation="MNSTAAADSPVSTADLALIERFLDALWLERGSAEPTLAAYRSDL
                     TALATWLTQQQGRLLDLDRAQLLDYLGERLAQGYSSRSTQRFLAALRNFTRYLLREGL
                     ISTDPALDIANPRLGRPLPVSLTEAEVEQLLAAPDVEQPLGLRDRTMLELLYATGLRV
                     SELVGLRHDQLDCQRGCVRITGKGGKERLIPVGEEALAWIERYRATTAPSRQDGRAPL
                     FPGRDGEALTRQAFWYRIKQHARQCGIDRPLSPHSLRHAFATHLLNHGADLRVVQMLL
                     GHSDLSTTQIYTHVARQRLRELHQKHHPRG"
     gene            complement(3226..4356)
                     /locus_tag="PKI23_10740"
     CDS             complement(3226..4356)
                     /locus_tag="PKI23_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37453.1"
                     /translation="MDFSFSEEQQLIKESVEKFIEKDYSFEARNKLLKTPERFSRALW
                     KQYAELGWLGIALPEEFGGYGGSAIETMLVMEAFGSAMVLEPYLSTVVMGANAVLFGG
                     SDAQKKTLLPKVASGELMLSVAYAEPTSRYELADVQTKATATAGGYSLSGEKIVVLNG
                     GTADKLIVSARTSGARLDQNGISLFLVDRNAAGVELSVYDTADGSQAANVRLNGATGE
                     LLGGEGQGHALLDQVIDTATAALCAQALGAMNSAFRRTLDYTKTREQFGVPIASFQVL
                     QHRLVDMFMSSEASRSMVYMVSMEVMKSDATARRKAVSAAKAYVGQHARAVAQDAVQI
                     HGGVGMTEELDVGHYFRCLTLFCSSFGSTDHHLQRYARLSQQ"
     gene            complement(4369..>4872)
                     /locus_tag="PKI23_10745"
     CDS             complement(4369..>4872)
                     /locus_tag="PKI23_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37454.1"
                     /translation="QNLVGEENQGWTVAKYLLTHERTSIAGVGAAKASLGKLKKLARA
                     TEANGKSMIEDPQFSLKLAQAELNVMALEYTNFRALAALASGQVPGAESSLLKIKGSQ
                     AQQMLQELKVEMSGYYAYPWQTEQAVGPVPFKQSLANYNFGRASTIYGGSTEVQYGVM
                     AKAILGL"
BASE COUNT          777 a         1490 c         1649 g          956 t
ORIGIN      
        1 gttttgcgcc acggtcgcgc cactggccca gttgctgttc gaggagtggc accagctctt
       61 tcagcgtggt gtcgccgacc acgatcaggg tggcacgacc gggctgaaac caggtgcggt
      121 ggaagttgac caggtcatcg cgcttcagcg acgagacgcc ggccacggtg ccgctgccgg
      181 tgaaggggag cgcatagggg tgtccctcgc cgtagagcag cggcggcatc agccgcatcg
      241 ccagcgagac cgggctggcc tcctcctgtt cgatccgggc cagccacagg gcacgcttgc
      301 gctcgatttc gctgcttttg aaggcggggt tgagcagcac gtcggcaaag agtccgagcg
      361 atgaggtgag gttgctcttc agcgtcgaga ggtgcaggct ggaggtgtcg atggtcgagc
      421 tggtggtgag ggtggcgccc agccgctcca gccggtcggc cagcgccagt gcgtcgtact
      481 ggctggagcc ttcggtcagc atctccatcg caaagctctg ggtgccgggc ttgccacctt
      541 ggtcggtcga gtggccggcg tcgaattgca gcgtcagttg caccagcggc acttcgtggc
      601 gttgggccag caccaccttg aggccatttt tcagcgtggc ccgttccaac tccggaatct
      661 tgagctgcgg gggttcatcg acagccggca gttggctgcg gtcggccccc tccttggccg
      721 cttcgagggt cgggaagggc tcgacctcca gggtgtagcg gccatcgctc agccatttcc
      781 tgctcagatc gcgcagggcg atcggggtgg cggcgttgat ctgcttcagc cagatgtcga
      841 ccatggcggt cgggttgtcg aaaaagactt cgccactggc cagcaggtcc gacttgccgc
      901 cagtgccgcc aacccgttcg gcctcgcgca cgaactgtgc ggtgtaggcc gccctgaccc
      961 gctcgagctc gctctggccc ggaccctggg tcagcagcgt ggtcagctcc tggtcgatgg
     1021 ctttttcgac ctcggcgagg gtgtggcctt tcttgacatc ggcggtgatg acgaagagcc
     1081 cggagagttc acggccccag taggcggcgc tcacctcggt cgcgatctgg tcgcggtaga
     1141 ccagccgctg atagagccgg gagttcttgc caccggccag cagttgaccc agcaggtcga
     1201 acagcggggc attctgatca cgcagttcgg gcacattcca gacctgatgg agccgtggct
     1261 gcggcacccg atcctgcatc acctcgcgct gatggccagt gcgctgggcg gtccaggcct
     1321 tggggcgggt gatcggctgg ccgggtgcga tgtcgccaaa gtagtgctgg accttctgtt
     1381 tggcggtctc gagatcgatg tcgccggcca gcaccagcac cgcgttggag gggccgtagt
     1441 aatccttgaa ccactggtgc acatcatcga gggtggcggc gttgagatcg gtcatcgaac
     1501 cgatcacctc ccacgaatag ggatggccgg ccggatagca ggccttggtg atcgcctcga
     1561 acacccggcc atagggctgg ttttcgccct ggcgcttctc gttctgcacc acgccgcgct
     1621 gctcatcgag cttggcctgg gtgatggcgc ccagcagatg gcccatgcgg tccgactcca
     1681 tccacagcgc caggtcgagc gcggaggtgg gcacgttctg gaaatagttg gtgcggtcgt
     1741 tgttggtggt gccattcatc tcggtggcgc cgacccgctc gaagggtttg aagtactcat
     1801 cgtcatggtg ttcgctgccg ttgaacatca ggtgttcgaa cagatgggcg aagccggtct
     1861 tgcccggctt ttcatccttg gagccgacgt gataccagag gttgaccgcc acgatcggcg
     1921 ccttgcggtc ctcatgcacg atcaccgtca gtccgttgtc gaggatgaat ttctgatggc
     1981 tcaggttcat tttgagcggt tcggcctgca ccgtgacgcc gcaggccagc agatggaaca
     2041 gcatgaacag ggaaggcagc agggagaagc gcatgcgcag ggtcatcatg gtggtcgatg
     2101 ggttgaagag ggtccgaaac gcccgccatg gtactgcgcc ttgcgtgccg gagtaaggct
     2161 tggggcacaa ctttgctgca tttctgggcc gaatcagccg cgcggatggt gcttctggtg
     2221 cagctcgcgc agccgctgtc gcgccacatg ggtgtagatc tgtgtggtcg acaggtcgct
     2281 gtgccccagc agcatctgca ccacccgcag atcggcacca tggttgagca gatgggtggc
     2341 aaaggcgtgg cgcaagctgt gcggtgacag ggggcggtcg atgccgcatt gtcgggcatg
     2401 ctgcttgatg cgataccaga aggcctggcg ggtgagcgcc tcgccgtcgc gacccgggaa
     2461 cagcggagcc cggccatcct ggcgtgaggg cgcagtggtc gcgcggtagc gttcgatcca
     2521 ggccagcgcc tcttctccga ccggaatcag ccgctccttg ccgcccttgc cggtgatgcg
     2581 cacgcagccc cgctggcagt cgagctgatc atggcgcaat cccaccagtt cggagacccg
     2641 cagacccgtt gcatagagca gttcgagcat ggtccggtca cgcagtccca gcggttgctc
     2701 gacgtccggc gcggccagca gttgctcgac ctcggcttcg gtcaggctga ccggcagcgg
     2761 gcggcccagt ctggggttgg caatgtcgag tgccgggtcg gttgaaatca gcccttcgcg
     2821 cagaagatag cgggtgaagt tgcgcagtgc cgccagaaag cgctgggtgg agcggctgct
     2881 gtagccctgt gccagccgct ctccgaggta gtcgagcagt tgggcgcggt cgaggtcgag
     2941 cagccggccc tgctgctggg tcagccaggt tgccagtgcc gtgagatcgc tgcggtaggc
     3001 cgccagggtc ggttcggccg aaccacgctc cagccagagg gcgtcgagaa agcgctcgat
     3061 cagcgccaga tcggccgtgc tgaccggtga atcagcggca gcagtgctgt tcatcgggcg
     3121 ctttgctcat gaagaggtgt tgtcgtcggg ttgcctccaa ataaaaagcc atgctccggc
     3181 actggccaga gcatggctgt tcgggctcac ccgacggatg cggcgctact gttgcgagag
     3241 ccgggcatac cgctgcaggt ggtggtcggt gctgccgaag ctgctgcaga agagcgtgag
     3301 gcaacggaag tagtggccga cgtcgagctc ttcggtcatg ccgaccccgc catggatctg
     3361 cactgcatcc tgcgccacgg cgcgggcatg ctggccgaca taggccttgg cggccgagac
     3421 cgccttgcgc cgtgcggtgg cgtcggactt catcacctcc atgctgacca tgtagaccat
     3481 cgaacggctc gcctcgctcg acatgaacat gtcgaccagg cggtgttgca gcacctggaa
     3541 gctggcgatc ggcacgccga actgttcacg ggtcttggtg tagtcgaggg ttcttctgaa
     3601 ggcggagttc atcgcaccca gcgcctgggc gcagagcgcc gcggtggcgg tgtcgatcac
     3661 ctggtcgagc agtgcatggc cctgaccttc gccgcccagc agttcgccgg tggcgccatt
     3721 gaggcgcaca ttggcggcct gcgagccatc ggcggtgtcg tagacgctca gctcgacacc
     3781 cgccgcattg cgatcgacca ggaacagcga gatgccgttc tgatcgagcc gcgcgccact
     3841 ggtgcgggcc gagacgatca gcttgtcggc cgtgccgcca ttgagcacga cgatcttctc
     3901 gccgctcagg ctgtagccgc cagcggtggc ggtggccttg gtctgcacgt ccgccagctc
     3961 atagcgggag gtcggctcgg cataggccac cgacagcatc agttcgccgc tggccacctt
     4021 gggcagcagg gtttttttct gtgcatcgct gccgccgaac agcaccgcat tggcgcccat
     4081 caccaccgtg gagaggtagg gttcgagcac catcgcgctg ccgaaggcct ccatcaccag
     4141 catggtctcg atggcactgc cgccgtagcc accgaactct tcgggcagtg cgatgccaag
     4201 ccagcccagc tcggcatact gcttccacag cgcgcggctg aagcgctcgg gcgtcttcag
     4261 cagcttgttg cgtgcctcga acgagtagtc cttttcgata aacttctcaa ccgattcctt
     4321 gatcagttgt tgttcttcgc tgaatgaaaa atccatcatt gccccctgtc agagcccaag
     4381 aatcgctttt gccatgacgc catactgcac ttcggtgctg ccgccataga tggtgctggc
     4441 gcggccgaag ttgtagttgg cgagagattg tttgaacggc accggtccga ccgcctgctc
     4501 ggtctgccat gggtaggcgt aatagcccga catctcgacc ttgagctcct gcagcatctg
     4561 ctgcgcctga ctgcccttga tcttcagcag cgaggattcg gcgcccggca cctgaccgga
     4621 ggcgagcgcg gccagtgcgc ggaagttggt gtactccagt gccatgacgt tgagctcggc
     4681 ctgtgccagc ttcaggctga attgcgggtc ttcgatcatc gacttgccgt tggcctcggt
     4741 ggcgcgggcc agcttcttca gcttgccgag cgatgccttg gcggcgccga cgccggcgat
     4801 gctggtgcgc tcgtgggtga gcaggtactt ggccacggtc cagccctggt tctcctcgcc
     4861 gaccaggttc tg
//
LOCUS       DAORCQ010000136         4814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_13300_length_4814_cov_6.044547, whole genome shotgun sequence.
ACCESSION   DAORCQ010000136 DAORCQ010000000
VERSION     DAORCQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13300_length_4814_cov_6.044547"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PKI23_10750"
     CDS             complement(<1..1177)
                     /locus_tag="PKI23_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNN37455.1"
                     /translation="MASLNQPAPDIWESLRKGFRLRDEMAHPRVAVQRRLLLEQSSFV
                     EKAAENSTRYLYHVKQALVERNFPLELAFLPMIESGYDPRARSASQATGMWQLIPSTA
                     RHLGLRKNTGYDGSRDVVESTEAALQYLDQMQKHFDGDWLLTLAAYNAGQGAIDRAIE
                     RNRALGKPTDFWHLNLPQQTMEFVPRILAFAQLAAEADKYQVALPNIPDRPYFVTVDI
                     ARQIELSVAAELAKLPLEEIERLNPGYRRGTTDSHGNHRLLIPAAQAEALNYKLSLFP
                     AERAAGWQHYKVKEGDSLESIAQRFDTSTTTIQQLNELDGSLRTGEKLVLPAPWSLLT
                     KRSESGTQGTAAQHSESARDSGEKGAQYRVRQGDTPWRIARQHGISLDELTRLNGEAK
                     "
     gene            1229..1531
                     /locus_tag="PKI23_10755"
     CDS             1229..1531
                     /locus_tag="PKI23_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37456.1"
                     /translation="MDLSTGCATGPVAVRSGVIRQSGGVLFDQAAASRQNRTEYECRQ
                     CLFETRQNEHLIDVDRPSRWVIVLGLRLCSPEWVSTPVEKLIHNSRGDDGGLVSIP"
     gene            complement(1521..2300)
                     /gene="gloB"
                     /locus_tag="PKI23_10760"
     CDS             complement(1521..2300)
                     /gene="gloB"
                     /locus_tag="PKI23_10760"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNN37457.1"
                     /translation="MLKTIPIPAFSDNYIWLLHEVDGAHAWVCDPGDAAVVERYLEAH
                     GLTLSGIFITHHHPDHIGGVAGLMAGRSIPVLGPRHEKIPHLTQPLGEGDVCQILGHE
                     VRILDIPGHTLGHIAYLIEGETALLFCGDTLFAGGCGRIFEGTVAQMYQSLMKLAALP
                     PQTAVHCAHEYTLSNLRFAAVVEPDNAQLQRRIVTESEKRGCGEPTVPSTIGLELATN
                     PFLRGHVAEVKRAAEAWKQGPLAQQEEIFGAIRHWKDGFKG"
     gene            2425..3213
                     /locus_tag="PKI23_10765"
     CDS             2425..3213
                     /locus_tag="PKI23_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNN37458.1"
                     /translation="MVKTMPQSTLHTRVMLEQAQRWFTSPLGQELLEIEQRLLDQILP
                     SCHGDLLVEISPFSTQFDYERCPITQQCRLSPLLHPHCPPPALVAEPTALPLYPESAS
                     VVLLHHLLEFCADPHRTVREAVQTLAPEGRILIIGFNPRSLWGLWQRIAAHRGSGPWS
                     GHFLTASRMEDWLSLLRCQPEGALHDRFGLPLQPRAWSRHWRGMSQIARQHHWPIGAI
                     YLLVARKKRAALIAQPIARRRLRLAAVGTPKLVMGQVSKSESHE"
     gene            3206..3685
                     /gene="rnhA"
                     /locus_tag="PKI23_10770"
     CDS             3206..3685
                     /gene="rnhA"
                     /locus_tag="PKI23_10770"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNN37459.1"
                     /translation="MSEKVEIYCDGACRGNPGPGGWGALLRCNGHEKRLYGSEPLTTN
                     NRMELQAAIAGLAALKRPCHVSITTDSKYVQQGMTEWLANWKKRGWKSASRQPVKNVD
                     LWMELDQQSSRHLRVDWHWIKGHSGHAENELADQLANQAIDELLGGGANKKVQGEAR"
     gene            3682..4410
                     /gene="dnaQ"
                     /locus_tag="PKI23_10775"
     CDS             3682..4410
                     /gene="dnaQ"
                     /locus_tag="PKI23_10775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNN37460.1"
                     /translation="MSRQVVLDTETTGLEARLGHRVIEIGAVEIINRRPTGRHYHQYL
                     NPERAVDAGALAVHGLGDEFLADKPLFAEIAAEFLQFIDGAELIIHNAPFDLEFLNRE
                     LELLNREPRRIEQLCAITDTLKLARNRHPGQKNSLDALCTRYGVDNSARQLHGALLDA
                     EILADLYLLMTSGQVSLSLDEAQSAAIHPGSQTMVEQLTQLAADRPRLPVIAASAEEL
                     ARHEAALDAIARECRGEPLWRRLL"
     gene            4520..>4814
                     /locus_tag="PKI23_10780"
     CDS             4520..>4814
                     /locus_tag="PKI23_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNN37461.1"
                     /translation="MIHTFACPDTETPFHSQSVPRFRNIERVARRKLLQLHAATELAS
                     LKVPPGNQLEALKGDRTGQHSIRINDQWRLLLWQQDGAGHGGIARRNPPRRNPA"
BASE COUNT          948 a         1589 c         1393 g          884 t
ORIGIN      
        1 ccttggcctc gccattcaga cgcgtcagtt cgtccagcga aatgccatgc tgtctggcga
       61 ttcgccacgg ggtgtcaccc tgcctgactc gatattgcgc gcccttttcg ccgctgtctc
      121 gggctgactc ggagtgctgt gcagcggtgc cttgcgtgcc actctcgctg cgcttcgtca
      181 gcagactcca gggcgccgga agcaccagct tctctccggt tctgagcgag ccgtccagtt
      241 cgttgagctg ctggatcgtt gtcgtcgaag tgtcgaaacg ctgtgcgatc gactccagcg
      301 aatcaccctc cttcaccttg taatgctgcc agcccgcagc acgctcggca ggaaagagcg
      361 acagcttgta gttgagtgcc tccgcctggg cggccggaat cagcagccga tgatttccat
      421 gagagtcggt ggtcccgcgt ctatagcccg gattgagccg ttcgatctcc tccaacggca
      481 gtttggccag ttcggccgca accgaaagtt cgatctgtcg tgcgatgtcg accgtcacga
      541 aatagggtcg atcagggatg ttcggcaacg caacctgata tttgtccgcc tcggcggcca
      601 gttgggcaaa ggcaaggatg cgcggcacga actccatggt ctgctgcggc agattcagat
      661 gccagaaatc ggtcggcttg cccagcgcgc ggttgcgctc gatggcacgg tcgatggcgc
      721 cttgaccggc gttgtaggcg gccagcgtca gcagccagtc gccgtcgaaa tgcttctgca
      781 tctgatcgag atattgcagc gctgcctcgg tcgactccac cacatcacgg ctgccgtcat
      841 agccggtatt cttgcgcaaa cccaaatggc gcgcggtgga gggaatcaac tgccacatgc
      901 ccgtggcctg actcgccgag cgtgcacgcg ggtcgtaacc cgattcgatc atcggcaaaa
      961 aggccagctc cagcggaaag ttacgctcga ccagtgcctg cttgacgtgg tagagatagc
     1021 gcgtcgaatt ttccgcagcc ttctcgacaa agctcgactg ttcaagcagc aagcgccgct
     1081 gcacagcaac ccggggatgc gccatctcgt cacgcagcct gaagcctttg cgcagtgatt
     1141 cccagatgtc aggcgccggc tggttcagcg aagccacctc tggcgcaaac cagttgtagt
     1201 ctcctgccgt gccattctcg atggcctggt ggatctctcc accggttgcg ccaccgggcc
     1261 ggttgccgtt cgcagcggag tgatccgcca gtcgggagga gtcctgttcg accaggctgc
     1321 agccagtcgt cagaaccgta cagaatatga gtgcagacag tgtctgtttg aaactcggca
     1381 aaatgaacac ctcatcgatg tcgatcgccc aagccgctgg gtgatcgtct tagggttgcg
     1441 cctttgcagc cctgagtggg tttctactcc agttgagaaa ttgattcaca acagccgtgg
     1501 tgatgacggt ggccttgttt ctatcccttg aaaccatcct tccagtggcg gatggcgcca
     1561 aaaatctctt cttgctgggc aagtggaccc tgtttccaag cctccgccgc ccttttgacc
     1621 tcggccacgt ggcccctcag aaaagggttc gtagcaagtt cgaggccgat tgtcgacgga
     1681 accgtcggtt cgccgcaacc tcttttttca ctttccgtga cgatccggcg ctgcaactgt
     1741 gcgttgtcgg gctcgaccac cgccgcaaaa cgcaggttgc tcagcgtgta ttcatgcgcg
     1801 caatggaccg cggtttgtgg cggaagtgcc gccagtttca tcagcgactg gtacatctgc
     1861 gccacagtgc cttcaaaaat gcgtccgcaa ccgccggcaa acagcgtgtc tccacagaag
     1921 agtagcgctg tttcaccctc gatcaagtag gcaatgtgcc ccaaggtatg gcccggaatg
     1981 tccagaatcc gcacttcatg ccccaggatc tggcagacat caccctcgcc cagcggctgg
     2041 gtcagatggg ggatcttttc atgacgcggc cccagtaccg gaatcgaacg ccctgccatc
     2101 agtccggcca cgccaccgat gtggtcggga tggtggtggg tgatgaaaat gcccgagagc
     2161 gtcagcccat gcgcctcgag gtagcgctcg accacggctg catcgcccgg atcgcacacc
     2221 cacgcatggg ccccgtccac ctcgtgcaac agccagatat aattgtcgct gaaggctgga
     2281 attggtatcg ttttcaacat gatcatctcc tgactccact ggccggacga cacaggaaac
     2341 catggccatc gacatcagac aagaccagca cggcgccatg gatccaaaac tttcgcctga
     2401 cctcacaccc gagaactgat ccagatggtc aagacgatgc cccaatccac cctccacacc
     2461 cgggtcatgc tggagcaggc acagcgctgg ttcaccagcc cgctgggcca ggagctgctg
     2521 gagatcgaac agcgtctgct cgatcagata ttgccaagct gccatggtga cctgctggtc
     2581 gagatcagtc ccttttcgac ccagttcgac tacgaacgct gcccgatcac ccagcagtgc
     2641 cgactgagcc cgctgctgca tccacactgc ccgccaccag cactggtggc ggaacccacc
     2701 gccctgccgc tctatccgga gagcgccagc gtggtgctgc tccaccacct gctggagttc
     2761 tgtgccgacc cgcatcgaac cgtgcgcgaa gcggtgcaga ccctggcccc cgaaggccgc
     2821 atcctgatca tcggcttcaa cccgcgctca ttgtggggcc tgtggcaacg catcgccgcc
     2881 catcggggca gcggcccctg gtccggccac tttctgaccg cctcgcgcat ggaagactgg
     2941 ctgtcgctgc tgcgctgcca gcccgagggg gccctccacg atcggttcgg actgccgctg
     3001 caaccacgcg cctggtcccg tcactggcgc gggatgagcc agattgcgcg gcaacaccac
     3061 tggccgatcg gcgccatcta tctgctggtg gcacgcaaaa aacgggcggc gctgatcgct
     3121 caacccatcg cccgccgccg gctgcgactg gccgccgtcg gcacccccaa actggtcatg
     3181 ggccaggtga gcaagagcga atcgcatgag tgagaaggtc gaaatctatt gcgacggcgc
     3241 ctgccgcggc aatccgggtc ccggcggctg gggcgcgctg ttgcgctgca acggccacga
     3301 aaagcggctt tatggcagcg agcccctgac caccaacaac cgcatggagc tccaggctgc
     3361 gattgccggg ctggccgccc tcaagcggcc ctgccacgtc tcgatcacca ccgactccaa
     3421 atatgtccag cagggaatga cagaatggct ggccaactgg aaaaaacgcg gctggaagag
     3481 cgccagccgc cagccggtga agaatgtcga cctgtggatg gagctcgacc agcagagcag
     3541 ccgccacctg cgggtcgact ggcactggat caagggacac agcggccacg ccgaaaatga
     3601 actggctgat caactggcca accaggcgat cgatgaactg ctcggcggcg gcgccaacaa
     3661 aaaggtccag ggagaggccc gatgagccgt caggtcgtgc tcgacaccga aaccaccggg
     3721 ctcgaagccc gtcttggcca ccgcgtcatc gaaatcggcg ccgtcgagat catcaatcgc
     3781 cgcccaaccg gacgccacta tcaccaatac ctcaatcccg aacgggcggt ggacgccggc
     3841 gcactggccg ttcatggcct cggcgacgaa tttctggccg acaaaccgct gtttgccgaa
     3901 atcgccgcgg agtttctgca gttcatcgat ggcgcggaac tgatcatcca caacgccccc
     3961 ttcgatctgg agttcctcaa ccgcgaactg gagctgctga accgtgagcc ccgccgcatc
     4021 gagcagctct gcgccatcac cgacaccctg aagctggcac gcaaccgcca tccggggcaa
     4081 aagaacagcc tcgatgcgct ctgcacccgc tatggcgtcg acaactcggc ccggcaactc
     4141 cacggcgccc tgctcgacgc cgagatactg gccgacctct acctgctgat gaccagcggt
     4201 caggtcagcc tctcgctcga tgaagcacag agcgctgcca tccacccagg cagccagacc
     4261 atggtcgaac agctgacgca actggcagcc gaccgaccac gcctgccggt cattgccgcc
     4321 tccgccgaag agctggcccg ccacgaagcc gccctcgatg ccattgcccg cgaatgccgg
     4381 ggcgaaccgc tgtggcgaag gctgttgtag ggtcatcttt tcttgatccg atgcacccga
     4441 ttggcctatc agtggcggtg ggcgcgatga aggcactctt tccgcttgac atgttccgta
     4501 tcacggaaca tgttgatcca tgatccacac gttcgcctgt cccgacactg aaacgccttt
     4561 ccatagccag tcggtgccgc gattcaggaa catcgagcgc gtggcgcgac gcaagctgct
     4621 gcaactgcac gccgccaccg agctggccag tctgaaagtt cctcctggca accagctcga
     4681 agcgctgaag ggcgaccgca ccgggcagca cagcatccgg atcaacgacc agtggcgcct
     4741 gcttctctgg caacaggacg gagcgggaca tggcggaatt gctcgacgaa atccaccccg
     4801 gcgaaatcct gctg
//
LOCUS       DAORCQ010000137         4719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_13731_length_4719_cov_5.186321, whole genome shotgun sequence.
ACCESSION   DAORCQ010000137 DAORCQ010000000
VERSION     DAORCQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13731_length_4719_cov_5.186321"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PKI23_10785"
     CDS             <1..1184
                     /locus_tag="PKI23_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005744200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNN37462.1"
                     /translation="RRLILATASHRHYAEAIAEHLGLFEQVLATEGEVNLSAHHKRDL
                     LVQHHGEQGFDYLGNSRDDLKVWEAAQRAHLANPERGVEAAARRQGNVEQVLRTAGNP
                     WRDWSQQLRLHQWAKNALLFVPLLAAHKLGDAAMLLNGLLAFVLFGLCASSVYLLNDL
                     LDLQDDRQHHRKRHRPLASGAVPIKSVLLLFPLLTLTAFAGAAWLLPQQFVLALLCYY
                     GLTLSYSLLLKRYMGVDVIVLATLYTLRVIAGTFAFGVTLTFWMLAFSMFLFLSLALV
                     KRYTELRDSRLKGKSELTPGRGYYPADLEMVSALGAASGYLSVMVLALYTQDQTTAAL
                     YQQPQLIWLACPLLLYWVTRTWMLAHRGQMHEDPVLFAIKDRTSLLVGALFAAVFWIA
                     A"
     gene            1181..2500
                     /locus_tag="PKI23_10790"
     CDS             1181..2500
                     /locus_tag="PKI23_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010433186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNN37463.1"
                     /translation="MSAGRYSWGRYPHHPQTLHHCAWRETVRPTLAAVAAKHGSLLPY
                     GNGRSYGDSCLAASDQLLSLRPLDRFIAADWQSGLIRAEAGMTLGELLELAIPRGWFV
                     PVTPGTKFVTLGGALANDVHGKNHHRRGTFGCHVPRFGLMRSDGAELVCSSSENSALY
                     AATIGGLGLTGLIDWVELQLLPIRSSLLDVTTVRFGSLDEFFALSNELDQRHEFAVSW
                     IDCLSRGKAAGRGVYSVADHAEAGPLACHRPSRLRVPVTPPLSLVNRLSLRLFNSAYY
                     RLHRADRHQSRNSHEPFFYPLDRILNWNRIYGPHGFQQYQCVLPPGNAAAATRALLDS
                     IASAQNGSFLAVLKRFGDAPSPGLLSFPMPGVTLALDFPQQGQRTQRLFARLDAIVRE
                     AHGRLYPAKDAHMSGSDFRAAYPAWTQLEALRDPRILSRFWQRVIDQ"
     gene            2497..4482
                     /locus_tag="PKI23_10795"
     CDS             2497..4482
                     /locus_tag="PKI23_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37464.1"
                     /translation="MSRLSRSGWKQFLATRPVVLGALLLLASLLAFAPFAPLLPGTEL
                     DDAWRLAMNQAVAQGLVFGRDIVFTFGPYAALYTQHYHPATYPAALLLGVLFGLAQGT
                     LLLSCLRRRHNGWLWTLAGFLLLVDSRDALFFAYPLLLTLVTCMESASHGSDDRLRFV
                     GQETGHLLAIALLGALPIIKGSFWPLAVMASLVGALLHGLKGERLRAALFILLPLLTL
                     LLLWGLAGQPLAALPDYFRTLSPIISGFAEAMSNRGLLWQVVVYLGLAAGIILATLRS
                     AGPALPKRLAITLAVGLFLFIAFKAGFVRHDRVHAGAISTGTVLAILAIQATHSAKLP
                     RSIVVTALLMCLFMHLRYIGLSIEQTPLAALRGVTDNNPLDIRQAFTERMRQLATQSP
                     LPRLPGSSDIYPFDLTDLIASGNHWAPRPVLQSYSAYTPLLARMNEAHLRGAAAPDNI
                     FFRIESIDGRFPSLDDGLSWPTLLSHYQITGSDRQYLHLQRRTTPATTQKTVLTQQSH
                     RLGEWVSLPSAAQMLFAEIELAPTLLGRLSMLLFKPDQLYLSVDLGRGEKRVYRFIPS
                     MALAGFVLSPLVESTAEFARLANGQPPSLHDTRVVQRIRILSSRAVTRCGESLTFCEK
                     DPEKQEKGSNSFSWRSDYRLTLTALAIPAHSVSRQAQ"
     gene            4479..>4719
                     /locus_tag="PKI23_10800"
     CDS             4479..>4719
                     /locus_tag="PKI23_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37465.1"
                     /translation="MSQSTTVRAQPRRGRGADGRFESTEALARQLLDDSASTARPVTR
                     RSLLLLGGLLLLTSLLAFTPIVPNVEAIPRTAGLSM"
BASE COUNT          711 a         1686 c         1446 g          876 t
ORIGIN      
        1 gccgccgcct gattctggcg accgccagcc accgccacta tgccgaggcg atcgcggagc
       61 acctcggcct gttcgagcag gtgctggcca ccgagggcga ggtcaatctg tcggcccacc
      121 acaagcgtga cctgctggtg caacaccatg gcgagcaggg gttcgactac ctcggcaact
      181 cgcgcgatga cctgaaggtg tgggaggcgg cccagcgcgc ccacctggcc aacccggagc
      241 gcggtgtcga agccgcggcc agacggcagg gcaacgtcga gcaggtgctg cgcacagccg
      301 gcaacccctg gcgtgactgg agccaacagt tgcgcctgca ccagtgggcg aagaacgcac
      361 tgctgttcgt cccgctgctg gccgcccaca agctcggcga tgccgccatg ttgctgaatg
      421 gcctgctggc ctttgtgctg tttggcctct gtgcttccag cgtctatctg ctcaacgacc
      481 tgctcgatct gcaggatgac cgccagcacc accgcaagcg ccatcgtccg ctggccagcg
      541 gcgcggtccc gatcaagtcg gtgctgctgc tgtttccgct gctgacgctg accgcgttcg
      601 ccggcgcggc ctggctgctg ccgcagcagt tcgtgctggc gctgctctgc tactacggcc
      661 tgaccctctc ctactcactg ctgctgaagc gctacatggg ggtcgatgtc atcgtgctgg
      721 ccacgctcta cacgctgcgg gtcatcgccg gcaccttcgc cttcggggtc acgctgacct
      781 tctggatgct ggccttctcg atgttcctgt tcctcagtct ggcgctggtc aagcgctaca
      841 ccgaactgcg cgactcccgc ctcaagggca agagcgaact gaccccgggg cgcggctact
      901 acccggccga tctggagatg gtctcggcac tgggcgctgc ctccggctat ctctcggtga
      961 tggtgctggc gctctacacc caggatcaga cgaccgcagc cctctaccag cagccccagc
     1021 tgatctggct ggcctgcccg ctgctgctct actgggtcac ccgcacctgg atgctcgccc
     1081 accgcggcca gatgcatgag gatccggtgc tgtttgcaat caaggatcgc accagcctgc
     1141 tggtcggtgc cctcttcgcc gcggtcttct ggatcgctgc atgagtgccg ggcggtactc
     1201 ctggggacgc tacccgcacc atccacagac cctgcaccac tgtgcctggc gcgaaacggt
     1261 gcggcccacc ctggcggccg tggccgccaa acatggcagc ctgctcccct atggcaatgg
     1321 ccgctcctat ggtgacagct gcctggccgc cagcgaccag ttgctgtcgc tgcggccgct
     1381 cgaccgcttc attgcggcgg attggcagag cgggctgatt cgtgccgagg cgggcatgac
     1441 gctcggcgag ctgctggagc tcgccattcc gcgcggctgg ttcgtgccgg tcacgccggg
     1501 caccaagttc gtcaccctcg gtggcgcact ggccaatgac gtccatggca agaaccacca
     1561 tcggcgcggc accttcggct gccatgtgcc ccgcttcggg ctgatgcgca gcgatggggc
     1621 cgagctggtc tgctcctcca gcgaaaacag cgcactctat gccgccacca tcggtggtct
     1681 ggggctgacc ggtctgatcg actgggtcga actgcaactg ctgccgatcc gctccagcct
     1741 gctcgatgtc accacggtgc gcttcggctc gctcgatgag ttctttgccc tctcgaacga
     1801 actggatcag cggcacgagt tcgccgtctc ctggatcgac tgtctgtcac ggggcaaggc
     1861 cgccggacgt ggcgtctaca gcgtggccga ccatgccgaa gcgggcccgc tggcgtgcca
     1921 ccgcccctcc cggctgaggg tcccggtcac gccgccgctg tcgctggtca accgcctgtc
     1981 gctgcggctg ttcaacagcg cctactaccg gctccaccgg gcggaccgtc accagagccg
     2041 aaacagccat gagcccttct tctacccgct cgaccgcatc ctgaactgga accgcatcta
     2101 cggtccgcat ggttttcagc agtatcagtg cgtgctgccg cccggcaacg cggccgcggc
     2161 gacccgcgcg ctgctcgaca gcatcgccag cgcacagaac ggctcctttc tcgccgtgct
     2221 gaagcgcttc ggcgatgcgc catcgcccgg gctgctctcg ttcccgatgc caggcgtgac
     2281 cctggcgctc gacttcccgc agcagggtca gcgcacccag cggctgtttg cccggctcga
     2341 tgccatcgtc cgcgaagcgc acgggcggct ctacccggcc aaggatgccc acatgagcgg
     2401 cagcgacttc cgcgctgcct acccggcctg gacgcagctc gaagcgctgc gcgatccgcg
     2461 gatcctgtca cggttctggc aacgggtcat cgaccagtga gtcgactgtc gcgctctgga
     2521 tggaaacagt ttctggccac ccggcctgtc gtgctgggtg ctctgctgct gctcgccagc
     2581 ctcctggcct ttgcaccctt cgcgccgctg ttgcccggta ctgaactgga cgacgcctgg
     2641 cgcctggcga tgaaccaggc ggtggcgcag ggactggtgt tcgggcgcga catcgtcttc
     2701 acctttggcc cctacgccgc gctctacacc cagcactacc accccgcaac ctatccggcc
     2761 gccctgctgc tcggcgtgct gttcgggctg gcacagggca ccctgctgct gagctgcctg
     2821 cgccgacggc acaacggctg gctgtggacc cttgccggct ttctgctgct ggtcgactcg
     2881 cgcgatgcgc tgttcttcgc ctacccgctg ctgctgaccc tggtgacctg catggagagt
     2941 gcctcccacg gcagtgatga ccggctgcgc tttgtcggcc aagagaccgg ccatctgctc
     3001 gccatcgccc tgctcggcgc gctgccgatc atcaagggct cattctggcc gctggcagtg
     3061 atggccagcc tggtcggtgc gctgctgcat ggcctgaagg gtgagcggct gcgtgcggcg
     3121 ctgttcatcc tgctgccgct gctgacgctg ctgctgctgt gggggctggc cggtcaaccg
     3181 ctggcggcgc tgcccgacta cttccgcacc ctgtcgccga tcatctccgg ttttgccgag
     3241 gcgatgtcca accgcggact gctctggcag gtggtggtct acctcggact ggcggccggg
     3301 atcatccttg caaccctgcg ttcagccggc cctgcgctgc cgaagcggct ggccatcacc
     3361 ctggccgtcg ggctgttcct gttcatcgcc ttcaaggccg gtttcgtgcg gcatgaccgc
     3421 gtccatgcgg gggcgatcag caccggaacg gtgctggcca ttctggcgat tcaggccacc
     3481 cacagcgcaa agctgccgcg gtcgatcgtg gtgaccgcct tgctgatgtg cctgttcatg
     3541 catctgcgat acatcggcct ctcgatcgaa cagaccccgc tggcagcgct gcgtggtgtg
     3601 acggacaaca atcccctcga catccgacag gcattcaccg agcggatgag acagctcgct
     3661 acccagtcac ccctgccccg actccccggc agcagcgata tctacccctt cgacctcact
     3721 gacctgatcg cttcgggcaa ccactgggca ccacgtccgg tgctgcagag ctattcggcc
     3781 tacaccccgc tgctcgcccg gatgaatgaa gcgcatctgc gcggcgcagc ggcgccggac
     3841 aacatcttct ttcgcatcga gagcatcgat ggccgcttcc cctcactgga cgatggtctg
     3901 agctggccga cgctgctgtc gcactaccag atcacgggca gcgaccgcca atacctccac
     3961 ctgcaaagac gaaccacgcc ggccacgacg caaaaaaccg tgctgacgca gcagagccat
     4021 cgccttggcg aatgggtgtc gctcccctct gcggcgcaga tgctctttgc cgagatcgag
     4081 ctggcgccga cgctgctcgg gcgactgtcg atgctgctgt tcaagcccga tcagctctac
     4141 ctctcggtcg acctcggccg tggcgagaag cgggtctatc gcttcattcc cagcatggcg
     4201 ctggccggct tcgtgctgtc accgctcgtc gaatccaccg ccgaattcgc ccgactggca
     4261 aatggtcaac cgccatcgct gcacgacacc agagtggtcc agcgcatccg catcctctcc
     4321 tcccgcgccg tcacccgctg cggcgagtcg ctgacgttct gcgaaaaaga tccagaaaag
     4381 caagaaaaag ggagcaattc gttctcctgg cgcagtgact accgtctgac gctgacggca
     4441 ctggcgattc cggcccattc cgtttcacgg caagcacaat gagccaatcc acgacagtgc
     4501 gggcgcagcc tcgacgaggc agaggagccg atggccgatt cgagtcgacc gaggcactgg
     4561 ctcggcaact cctcgacgac agcgcgagca ccgcgcggcc ggtgacccgg cgctcgcttc
     4621 tgctgctggg cggcctgctg ctgttgacca gcctgttagc cttcacgccg atcgtgccga
     4681 acgtcgaggc catcccgcgc accgcgggct tgtccatga
//
LOCUS       DAORCQ010000138         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_14279_length_4601_cov_4.568192, whole genome shotgun sequence.
ACCESSION   DAORCQ010000138 DAORCQ010000000
VERSION     DAORCQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14279_length_4601_cov_4.568192"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKI23_10805"
     CDS             complement(<1..309)
                     /locus_tag="PKI23_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNN37466.1"
                     /translation="MYIYDHYDQQLVNERVRQFRRQTERYLDGALNDDQFRPLRLQNG
                     LYIQRVAPMLRIAIPYGLLSSTQLRKLATICRHYDKGYGHFTTRQNLQLNWPELEDVP
                     D"
     gene            570..1706
                     /locus_tag="PKI23_10810"
     CDS             570..1706
                     /locus_tag="PKI23_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNN37467.1"
                     /translation="MSQITWSDEHHAFRAAFAKFLDTEAVPYHAEWEKKGQVDRAIWT
                     KAGQNGFLCYTLPEQYGGSGVDFRYTLIAVEEVSRRSLSGLGFGVHSDIVAPYILKHS
                     ISEEQKMRYLPKLASGEMVGAIAMTEPGAGSDLQSIRTTAILDGDHYVVNGSKTFITN
                     GQHCDLLILVCKTDPTLGAKGVSLLLVEANTPGFVKGRNLDKIGMKAQDTSELFFQDM
                     RVPKENLLGEAGKGFGYLMTELPQERLTIGAGAISSAEVALERTLEYTKERKVFGKSV
                     ADFQYNRFKLAEMATEITIGRSFLNHCIELHVNRQLDAVTAAKLKWWSTELQNRVIDQ
                     CLQMHGGFGYMWEFPIARQFADSRVSRIYGGSNEVMKEIIARTL"
     gene            1776..2882
                     /gene="tal"
                     /locus_tag="PKI23_10815"
     CDS             1776..2882
                     /gene="tal"
                     /locus_tag="PKI23_10815"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNN37468.1"
                     /translation="MSDNPLQQLRALGQSPWFDYIRRELITSGELARLIESSALGGIT
                     SNPSIFEEAITRHHDYDAAIAARLREPVTAEQLYHALVVEDVSLAAAAFLPLYRQTAA
                     ADGYVSLEVSPHLADDSATTLQQARQLWHEIDAPNLMIKVPATQAGLPAIRQLIAEGI
                     NVNVTLLFGLERYRAVLDAFTAGLEQRQQQGLPLTGVASVASFFISRIDTLIDPQLDR
                     LAVNDPQATQWRGESAIALARLAYRHYQQWRNSPRWQRLAAAGAQPQRLLWASTSTKD
                     PAYADTKYVEALIGPETVNTLPPATLAAYRDHGQPAVRIDQDEPPAQALVAALTRLGI
                     DLEQVAEQLEQEGVLKFVEAHDRLLAALAARCGG"
     gene            2971..4596
                     /gene="zorA"
                     /locus_tag="PKI23_10820"
     CDS             2971..4596
                     /gene="zorA"
                     /locus_tag="PKI23_10820"
                     /inference="COORDINATES: protein motif:HMM:NF033916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage ZorAB system protein ZorA"
                     /protein_id="HNN37469.1"
                     /translation="MLLEFSPKFCIEPCRPIPHVDVLPGISWSMAGAMNWIEEQLQLT
                     QAWDWILQAIQSLLTNPGLGSWVQLLLLILFSAFIAVFLFPGLFLRWRLGQIRRSLVK
                     TKQAKNSISPADLDRLFSDDKKLLHLWEEFKDTLHAQKEERDGLIISGAWRATAPAES
                     FFNIQSVAEGRLRTEFFKHLPGIFTGIGIIGTFSGLILGLQNFEVPDDPAKVRSSLDG
                     LLHGVFEAFIVSATAIGLAMLVTFLEKALLASLYRCTEEIAQHLDGLFTMGAGEEYLA
                     RLVLASEDAAAQSKILKDALVGDLKVILQEMTDRQVAASAAHSEALGRQITSGIETSL
                     QSPLEKIGEIVAKASGEQTTAATDLLKDVMASFGQRLNDLFGGQISGIQELNQKSAQA
                     MQDAVASLNNLVGKMAENSERSGNAMADKMASAIEEMERRQAEINGQTRSLVESIRDI
                     VSKSQNETNAELQRAVAALGAQMHEMIGALQEQATRSHEEQQQRERSFVETTSGEVGR
                     IGESVAQVIEQMVDATEQMKRSVTFVQAGLGPQ"
BASE COUNT          982 a         1358 c         1389 g          872 t
ORIGIN      
        1 atcgggcaca tcctcgagtt cgggccagtt gagttgcaga ttctgccggg tggtgaagtg
       61 gccatagccc ttgtcgtagt ggcggcagat ggtggccagc ttgcgcaact gggtcgagct
      121 gagcagaccg taggggatgg cgatgcgcaa catcggcgcc acacgctgga tgtaaagccc
      181 gttctgcagc cgcagcgggc ggaactggtc gtcgttcaat gcgccatcga ggtagcgttc
      241 ggtctggcga cggaactggc gaacccgctc gttcaccagt tgctgatcat agtggtcgta
      301 gatgtacatg gtgcaagatt cagcaggtcg atcggatcgg tgttttcttc gagcccaaaa
      361 gcaagcccgc aaggataaca gggaaggcgc cccggcaaaa acgccttttc ggtggggaga
      421 tcacccgcca cggcaggctg acctgttgca gcgggcatga cacaatatcg gcgcgctgaa
      481 ccgagtgaac tggcaaggca aacctgcacc agctcgagca caaggcgtgt tacccccatc
      541 aattcactgg acaatgaacc ggagtcgaca tgagtcagat cacatggagc gatgagcatc
      601 acgcctttcg tgcagcattc gccaagtttc tcgataccga ggccgtgccc taccacgccg
      661 agtgggaaaa aaagggacag gtcgaccgcg ccatctggac caaggcgggt cagaacggct
      721 ttctctgcta caccctgccc gagcagtatg gcggctccgg ggtcgacttc cgctatacgc
      781 tgatcgcggt cgaagaggtg tcgcgccgtt cactctccgg tctgggtttc ggtgtgcact
      841 ccgacatcgt cgcgccctac atcctcaagc acagcatcag tgaagagcag aagatgcgct
      901 acctgcccaa gctggccagc ggcgagatgg tcggcgccat tgccatgacc gaaccgggtg
      961 ccggttccga tctgcagagc atccgcacca cggcgatcct cgatggtgac cactacgtgg
     1021 tgaatggctc caagaccttc atcaccaacg gccagcactg cgacctgctg atcctggtct
     1081 gcaagaccga ccccacgctg ggcgccaagg gcgtcagcct gctgctggtc gaagccaaca
     1141 cccccggctt cgtcaaggga cgcaacctcg acaagatcgg catgaaggcg caggacactt
     1201 cggaactgtt cttccaggac atgcgggtgc ccaaggagaa cctgctgggt gaagccggca
     1261 aggggttcgg ctacctgatg accgaactgc cgcaagagcg gctcaccatc ggtgccggcg
     1321 ccatctcctc ggccgaggtg gccctggagc ggacgctgga atacaccaag gagcgcaagg
     1381 tgttcggcaa gtcggtcgcc gacttccagt acaaccgctt caaactggcg gagatggcca
     1441 ccgagatcac catcggccgc agctttctca accactgcat cgaactgcac gtcaaccggc
     1501 agctggacgc cgtgaccgcc gccaagctca agtggtggag caccgaactg cagaaccgcg
     1561 tcatcgacca gtgcctgcag atgcatggcg gtttcggcta catgtgggag ttcccgattg
     1621 cccggcagtt tgccgattca cgggtgtcgc gcatctatgg cggcagcaac gaggtgatga
     1681 aggagatcat cgcccgcacc ctttgatgcc gaagtgcccc gcatggcgca ccgtccatgc
     1741 ggggcccgct gaccgacgac ccgaggtgaa gtgagatgag cgacaacccc ctgcaacagc
     1801 tgcgtgcact gggccagagc ccctggttcg actacatccg ccgcgaactg atcacctccg
     1861 gtgaactggc acggctgatc gagagctcgg ccctgggcgg catcacctcc aacccatcga
     1921 tcttcgagga ggcgatcacc cggcaccatg actatgacgc agccatcgcg gcccgactgc
     1981 gcgagccggt caccgccgag cagctctacc atgcgctggt ggtcgaggat gtttcgctgg
     2041 cggcagcggc cttcctgccg ctctatcggc agacggcggc ggccgatggc tatgtcagcc
     2101 tcgaagtttc accccacctc gccgatgaca gcgcgacaac cctgcaacag gcgcggcagc
     2161 tctggcatga gatcgacgcg cccaatctga tgatcaaggt gcccgccacc caggccgggc
     2221 tgccggccat tcgccagttg attgccgaag gcatcaacgt caatgtcacg ctgctgttcg
     2281 gtctggagcg ctatcgggcg gtgctcgacg ccttcaccgc cgggctcgaa cagcgccagc
     2341 agcaggggtt gccgctgacc ggcgtcgcct cggtggccag cttcttcatc agtcgcatcg
     2401 acacgctgat cgatccacaa ctcgaccggc tggccgtcaa cgaccctcaa gctacgcagt
     2461 ggcgtggaga gagcgccatt gcgctggccc gactggccta ccggcattac cagcagtggc
     2521 ggaattcccc ccgctggcag cggctggccg ccgccggtgc gcagccgcaa cggctgctgt
     2581 gggcctccac ctcgaccaag gatccggcct atgccgacac caaatatgtc gaggcgctga
     2641 ttggcccgga gaccgtcaac accctgccgc cggcgacctt ggccgcctac cgcgaccatg
     2701 gccagccggc cgtgcgcatc gatcaggatg aaccgccggc gcaagcgttg gtggcggcgc
     2761 tgacgcgact cggcatcgat ctggagcagg tggcggagca actggagcag gagggcgtgc
     2821 tcaagttcgt cgaggcccac gaccgcctgc tggcggcact ggcggcgcgc tgcggcgggt
     2881 gatgatgttt tctgccattg gggcgcgata tgaggcatcc cattgctggc catctcgtgt
     2941 tggctcgaag gatggtccag agcatccgac gtgctgctgg agttttcacc aaaattttgc
     3001 atcgagcctt gccgccccat tccacacgtg gatgtgttgc caggcatttc atggagtatg
     3061 gctggcgcga tgaactggat tgaagaacaa ttgcaactga cacaagcatg ggactggatt
     3121 ctgcaagcga ttcagtcact tctcaccaat cccggcttgg gctcctgggt gcagttgctg
     3181 ctactgatac tcttttctgc cttcatagct gtatttctat ttcctggact gtttttgcgc
     3241 tggcgtttgg gtcaaatccg cagatctctc gtcaaaacaa aacaagccaa gaattcaatc
     3301 tccccagcgg atctggatag gttgttttcg gatgataaaa aactgctgca tttatgggag
     3361 gagttcaaag acacgcttca tgctcagaaa gaagagagag acgggctaat catctccggt
     3421 gcttggcgtg ccaccgcgcc ggcggaatca tttttcaaca tccaatcagt cgcggagggt
     3481 cgactacgga ccgaattctt caagcattta cccgggattt ttacgggtat cggtatcatc
     3541 ggcacgtttt ctggcctcat ccttggtctg caaaatttcg aggtgcctga cgatcctgcc
     3601 aaagttcgat caagtctgga tggcttgttg catggtgttt ttgaggcatt cattgtctct
     3661 gccacagcca ttggcttggc aatgctggtg acctttctcg aaaaggcgct gctcgcatca
     3721 ctttaccgtt gcacagaaga gattgcccag catctggacg gcctgttcac gatgggtgcc
     3781 ggagaagagt atctcgcgcg gctggttctg gcgtcagagg acgcggcggc acaatcgaaa
     3841 atcctcaagg atgcactcgt cggcgatctc aaggtcatcc tgcaggagat gacagatcgt
     3901 caggttgccg cgagcgctgc acactccgaa gccctgggca gacagatcac cagcggcatc
     3961 gaaaccagtt tgcagagtcc attagagaaa attggcgaaa ttgtggccaa ggccagtggt
     4021 gaacagacca cagcagcgac ggatttgctg aaggacgtga tggcaagctt cggtcagcgt
     4081 ttgaatgacc tgttcggtgg gcagatttcc ggaattcagg agttgaatca aaaaagtgct
     4141 caggcaatgc aggatgcagt cgcatccttg aataatctag tcgggaaaat ggccgaaaac
     4201 agcgagcggt caggtaacgc aatggccgac aagatggcca gtgcaatcga agaaatggag
     4261 cgtcgacaag ccgaaatcaa tggacagacg cgcagcctcg tcgaaagcat ccgtgacatc
     4321 gtttcaaagt cgcaaaacga aaccaacgca gagctccaga gagccgtcgc tgctctcggc
     4381 gcacagatgc acgaaatgat tggcgcgtta caggaacagg cgacgcgatc gcatgaagag
     4441 cagcaacagc gcgagcgcag cttcgtcgaa acgaccagtg gcgaggttgg caggatcggc
     4501 gaatcggtag cacaggtcat cgagcaaatg gttgacgcca ctgagcagat gaagcgcagc
     4561 gtcactttcg tccaggcggg cttgggcccc cagtaggcgt c
//
LOCUS       DAORCQ010000139         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_15622_length_4348_cov_3.547636, whole genome shotgun sequence.
ACCESSION   DAORCQ010000139 DAORCQ010000000
VERSION     DAORCQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15622_length_4348_cov_3.547636"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="PKI23_10825"
     CDS             <1..1610
                     /locus_tag="PKI23_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37470.1"
                     /translation="GTILIEQQEIPAEMVKSTVGGYAAERMQAISQQVMTTANLGGII
                     QKYNLYADERQRDTLATVVEQMREEIVLTPVSANVVDPKSGKPTTATIAFTLAYSNKS
                     PELAQRVANELATLFLNENLKQRTQQTREASRFLAAEAEKWQAQVTQLENELSSFKGK
                     YGEVLPELAQINQQAMQRAEQQLSEFDQRIRVQEDRRIALQGQLAQLSPNSTLYSTEG
                     KPILGNESRLKSLEAEYVSRSASYGPDHPDIIKMKKEIEALRKEVASTETAASLAARQ
                     QQQQAELQSLRERYSADHPDVKRLERQIAETESALSRASSQQSQRAALASKPDNPVYI
                     QMQAQLQAAESELKSLQLSKKQAEAKIGELEQRMKQMPEVEQQYRKLTADLDSARKSH
                     ADISEKQVQAQLSEVLEKEQKGERFTLVEPPPLPEDPASPNRPAILFLGLMLAAAAGV
                     GTVAMRENIDQSLYGAASVNDVLGISPLATIPYILTERDHASQRRQRRLILVMLLVLA
                     LVLVLVIHFFIMPLDIIWFSVLRRLGI"
     gene            1693..2538
                     /locus_tag="PKI23_10830"
     CDS             1693..2538
                     /locus_tag="PKI23_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN37471.1"
                     /translation="MERIKEALERAREERAGAGSSGGQPRARSDASRPVAVAPDEIEY
                     TQTRVIPLDSARLVERRIVAHLPAHPAADAYRVLRTQVLRRMTDNGWSTLMVTSPTEG
                     NGKTLTAINLAISMAREVNHTVLLVDFDLRRPTIGGFFSAAQMPGISDHLLHRQPLSE
                     MLFNPSIERLVVLPGHEALADSSSERLSSPQVVELVEEIKNRYPARIIIFDLPPLLQF
                     DDVLAFSPYVDAALLVVEEGATTDLQLRRSHELLQNVNLCGVVLNKSREESSAYGSGY
                     GYAHG"
     gene            2684..3616
                     /locus_tag="PKI23_10835"
     CDS             2684..3616
                     /locus_tag="PKI23_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNN37472.1"
                     /translation="MYEAHFGLKEKPFSILPDPSFLYLGRKHQMAFTLLEYAIENQAG
                     FAVITGEVGCGKTTLIRHLLNRLSGDITVGMITNTHESFGQLLQWILHAFGLDYGNSR
                     SKVTLYRIFTDFLIAEYAKGRRTLLIIDEAQNLSLAILEELRMLSNINADKDQVLQMI
                     LSGQPELKEKLKRQELRQFAQRVAVDYELLPLDVDETAHYITTRLQIAGRDAPLFTPE
                     AIELLYRHTQGIPRRINILCDMALVYGYAGQQQQIDAELLEEVIGNKAHTGIFTDPVP
                     LLSDVVPDADEAPTRRGANAKLDKQSLRDLFGKG"
     gene            3895..>4348
                     /locus_tag="PKI23_10840"
     CDS             3895..>4348
                     /locus_tag="PKI23_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37473.1"
                     /translation="MVQRIAALLLFWMVSILVTPVWAEQEPINVGLAVHLPLFMWDKN
                     HPTIDSVNQNQEMQRRWRYAEADLNIVQKIGAGWVVVSIRQWRDTPDDLLRVRRVIDQ
                     HRARGLKVVFRLLEEHAVYGRLTMTEDSTSGYDHDYYKWVRQLAERFGS"
BASE COUNT          920 a         1315 c         1339 g          774 t
ORIGIN      
        1 cgggcaccat tctgatcgag cagcaggaga ttccggccga gatggtcaag tcgactgtcg
       61 gcggctacgc ggccgagcgg atgcaggcca tcagccagca ggtgatgacc acggccaatc
      121 tgggcggcat catccagaag tacaatctct atgccgatga acgccagcgc gacaccctgg
      181 cgaccgtggt cgagcagatg cgtgaagaga tcgtgctcac gccggtcagc gccaacgtgg
      241 tcgatcccaa gagcggcaag ccgaccaccg ccaccatcgc cttcacattg gcctactcca
      301 acaaatctcc agagctggcg cagcgggtgg ccaacgaact ggccacgctg tttctcaacg
      361 agaacctgaa gcagcgcacc cagcagaccc gtgaggcttc ccgttttctg gccgccgaag
      421 cggaaaaatg gcaggcgcag gtgacgcagc tggagaacga gctgagcagc ttcaagggta
      481 agtatggcga agtgctgcct gaactggcgc agatcaacca gcaggcaatg cagcgggcgg
      541 agcagcagtt gagtgaattc gatcagcgca ttcgggtgca ggaggaccgg cgcatcgccc
      601 tgcagggcca actggcgcaa ctgagcccca acagcacgct ctactcaacc gaaggcaagc
      661 cgattctcgg caacgagagc cggctcaagt cgctcgaagc cgaatatgtc agccgttcgg
      721 ccagttacgg gccggaccac cccgacatca tcaagatgaa gaaagagatc gaggcgctgc
      781 gcaaggaggt tgcctcaacc gaaactgctg catcgctcgc tgcacgccag cagcagcagc
      841 aggcggagtt gcagagtctg cgtgagcgct attcggccga ccaccccgat gtgaagcggc
      901 tggagcggca gattgccgag accgagagtg cgctgagccg cgccagcagc cagcagagcc
      961 agcgtgcggc actggccagc aagccggaca acccggtcta catccagatg caggcacagt
     1021 tgcaggccgc cgagagcgag ctcaagtcgc tgcaactctc gaaaaagcag gccgaggcca
     1081 agatcggcga actcgagcag cgcatgaagc agatgccgga ggtggagcag cagtaccgca
     1141 agctgacggc cgatcttgac agcgcccgca agagccatgc cgacatcagt gagaaacagg
     1201 tgcaggcgca gctttccgaa gtgctcgaaa aagagcagaa gggcgaacgc ttcaccctgg
     1261 tcgagccgcc gccgctgcct gaagatccgg cgagtccaaa tcgtccggcg atcctgtttc
     1321 tcggtctgat gctggccgct gcggcgggcg tcggcactgt ggcaatgcgc gagaacattg
     1381 atcaatcact gtacggggcc gccagtgtca acgacgtgct gggcatctcg ccgctggcga
     1441 ccatccccta catcctgacc gagcgtgatc atgccagcca gcggcgtcag cggcgattga
     1501 ttctggtgat gctgctggtg ctggcgctgg tgctggtgct ggtgattcat ttcttcatca
     1561 tgccgctcga catcatctgg ttcagcgtcc tgcggcggct ggggatctag cggcgagagt
     1621 tcatgaaggg gaagccgccg attccggctg gcttgccgtg tgacagtcca aacttctatt
     1681 caggtaaaag agatggaacg gatcaaagag gcgctcgagc gggcccgtga ggagcgcgcc
     1741 ggagcaggca gcagtggtgg ccagccccgg gcccgttctg atgcatcacg gccggtggcg
     1801 gtggccccgg acgagatcga atacacccag acccgggtga ttccgctcga ctccgcccgg
     1861 ctggtcgaac ggcgcatcgt cgcccatctg ccggcccacc cggcggccga cgcctaccgg
     1921 gtgctgcgga cccaggtgct gcggcgcatg accgacaacg gctggagcac gctgatggtc
     1981 accagtccga ccgaaggcaa tggcaagacc ctcaccgcca tcaacctggc catcagcatg
     2041 gcgcgcgagg tcaaccacac cgtgctgctg gtcgatttcg acctgcgccg gccgaccatt
     2101 ggcggctttt tcagcgctgc gcagatgccc ggcatcagcg accatctgct gcatcggcag
     2161 ccactcagcg agatgctgtt caacccatcg atcgagcggc tggtggtgct gccggggcat
     2221 gaagccctgg ccgactcctc atccgagcgg ctctcgtcgc cacaggtggt cgaactggtc
     2281 gaagagatca aaaaccgcta cccggcccgg atcatcatct tcgatctgcc gccactgttg
     2341 cagttcgacg atgtgctggc cttctcgccc tatgtcgatg ccgcgctgct ggtggtcgaa
     2401 gagggcgcga ccaccgatct gcagttgcgg cgctcgcacg agctgctgca gaacgtcaac
     2461 ctctgtgggg tcgtgctcaa caaatcgcga gaagagagct ccgcctacgg cagcggatat
     2521 ggctacgccc atggttgacc gccccgattc ggcctgtcgt cagcggcgcc tgagcagggc
     2581 gatgcgccat ttctggcagg ctgtttgctg tttgagttga agtcgacccg aaacccctgt
     2641 acctcgatgc caaggcgccg gggccgcaaa cagaatcagc accatgtacg aagcccattt
     2701 cggactgaaa gagaaaccct tttcgatcct gcccgatccg tcgtttctct atctcggccg
     2761 caagcatcag atggccttca ccctgctcga atatgccatc gagaaccagg ccggctttgc
     2821 cgtcatcacc ggcgaggtcg gctgcggcaa gaccacgctg attcgccacc tgctcaaccg
     2881 gctgagcggc gacatcaccg ttggcatgat caccaacacc catgaatcat tcggccaact
     2941 gctgcagtgg atactgcacg cctttgggct cgactacggc aacagccgca gcaaggtcac
     3001 cctctaccgg atcttcaccg actttctgat tgccgaatat gccaagggac ggcgcacgct
     3061 gctgatcatc gacgaggcac agaacctgag cctggccatt ctcgaagagc tgcgcatgct
     3121 gtcgaacatc aatgccgaca aggaccaggt gctgcagatg atcctctccg gccagccgga
     3181 gctgaaagag aaactcaagc ggcaggagct gcgccagttt gcccagcggg tcgccgtcga
     3241 ctacgaactg ctgccgctcg atgtcgacga gaccgcccac tacatcacca cccgcctgca
     3301 gatcgccggc cgcgatgcac cgctgttcac ccccgaggcg atcgaactgc tctatcgcca
     3361 cacccagggc attccccggc gcatcaacat cctctgcgac atggcgctgg tctatggcta
     3421 tgccggacag cagcagcaga tcgacgcaga gctgctcgaa gaggtgattg gcaacaaggc
     3481 ccacaccggc atcttcaccg acccggtacc gctgctgagt gacgtggtgc cagatgcgga
     3541 tgaagccccg acccggcgtg gtgccaacgc gaagctcgac aaacagagct tgcgggatct
     3601 gtttggcaag ggttgaacgc aaggctgtcg atgtctggcg acttcacccc agtggcttgt
     3661 gggcaattgg cacacgctga cggtgacaaa tcagtgcaac gaaagcgtgg caggctgccg
     3721 ggcgcctgtc cggatcgagt gcggatccgg gccatgttca tgcacagaac atcgattgtt
     3781 tgatggagat cgcaggttgg gatttttcat gctgaaaatc ggaccgtgag cctttcacaa
     3841 ggctgaaggt tggctattgt ttagcctggt atggtccaac cgaggttttg aaagatggtt
     3901 cagagaattg ctgcccttct gctgttttgg atggtgtcga tcctggttac cccggtatgg
     3961 gcggagcagg agccgatcaa tgtcggcctt gcagtgcatc taccgctgtt catgtgggat
     4021 aaaaatcatc caaccattga ctctgtgaat cagaatcaag agatgcagcg gcggtggcgt
     4081 tatgctgaag ccgatctgaa catcgtgcag aagatcggtg ctggatgggt tgtagtgtcg
     4141 atacggcaat ggagggatac gccggatgac ctgttgcggg tcaggcgggt aatcgaccag
     4201 catcgtgccc gagggctcaa ggtggtgttt cggttgctcg aagagcacgc ggtctatggt
     4261 cgattgacga tgaccgaaga cagcacctct ggctacgacc atgactacta caaatgggtc
     4321 cggcagcttg ccgaaagatt cggcagtg
//
LOCUS       DAORCQ010000140         4298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_15915_length_4298_cov_5.023333, whole genome shotgun sequence.
ACCESSION   DAORCQ010000140 DAORCQ010000000
VERSION     DAORCQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15915_length_4298_cov_5.023333"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PKI23_10845"
     CDS             <1..1176
                     /locus_tag="PKI23_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNN37474.1"
                     /translation="RVAAAQWGQVLAQRLPTLDLGVAIERQERHGGLSTSIDQNLLSP
                     NLSLSYLLFDFGGRQARDESARATLLAALHSQNSSTQRLILAVLQAYYQLIANDAAVT
                     AARANEQTAETVLKAAQLRHQIGSVTRVDLLQAQSAYSQTRLNRQSAEGNARTARGVL
                     ANSIGLDADVPLAVVAPPQPKPDPIAEENIHLLIEQARGQRPDLAAAEAQVRAALANQ
                     RAVRSAGLPSLSLSLQQINSYPSVTPDSRDTQVGLQLSMPLFTGFADSYRLRASQAEI
                     ELQQARSELLDNQVALEVWQRYHALQTSRDTLSTTLDLLASAEAAAQMALGQYRSGVG
                     TLLNLLTLQAQLANARLQHIQAQYNWFSSRMALAQALGQLTPTSASEPASQPPPASR"
     gene            1184..2344
                     /locus_tag="PKI23_10850"
     CDS             1184..2344
                     /locus_tag="PKI23_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNN37475.1"
                     /translation="MSRTKLLVSLITVLLLGAAAVGGYGLWQKRTQQAQGEQFRIEQL
                     DRGEIVQIISANGTVNPVVMVNVGTQVSGTLQKIHVDFNAQVREGQILAELDPRLLQA
                     QVDQDEANLASGQANLKLQQANWKRLKTLYEQEYISRADFDAATQAREAAEAQVALQQ
                     AQLRRSRTNLSYTIIRSPVSGIVVSRLVDVGQTVAASFQTPVLFQIAKDLGQMQIDTS
                     VAEADLGNVHLGQEVSFYVDAFPDHRFVGEVKQIRLNPTTQQNVVTYNVVVAVDNPAG
                     KLSPGMTAYASLIVSRKPDVVRLPNAALRFRPPAEKEQEPQGRGGRSAAGAVVYQLRD
                     GKPVAVPVKTGSSDGRYTELVEGALTAGESVIVGLKRPAAESGSGGGTRRMF"
     gene            2344..3054
                     /locus_tag="PKI23_10855"
     CDS             2344..3054
                     /locus_tag="PKI23_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNN37476.1"
                     /translation="MSALIRTERLTKSYAGQNGKAAVEILHGIDFSIEAGEFVAIMGQ
                     SGSGKSTLMNLLGCLDVASSGHYWLDGEAVDQLSPDALALLRNRLIGFVFQGFNLLPR
                     SNLLDNVALPLLYAGSARQARHQRAEEMLAAVGLAGLGQRLPSQLSGGQQQRVAIARA
                     LANHPRLILADEPTGNLDSHTSDEIMALFSRLNREQRIAIVLVTHEADVAAYTDRLIR
                     LRDGRILDDGPTQALLQR"
     gene            3062..4297
                     /locus_tag="PKI23_10860"
     CDS             3062..4297
                     /locus_tag="PKI23_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNN37477.1"
                     /translation="MIFWPIFGEAWKAMGANRLRTFLTMLGMIIGVAAVILMLAFGRG
                     AQESVNKSIADMGTNLLMILSGSTTSGGLRMGMGSTPTLTIEDARAIAELPSIERASA
                     VMSGTAQLVQGGNNWSSLVYGVTPDYLEVRNWALASGNSFDEAALRSATQVALLGQTV
                     ADNLFPDGENPLGKTIRIKNMPFLVIGLLEVKGQSMEGRNQDDAVVIPVTTAQRRLFG
                     TRFRGSVQYIMAQAVSAELAKEAEQEITQLLRQRHQLGDNEQNRKPDDFTLRNLSELA
                     QTAAVAARAFALLLGAIASISLLVGGIGIMNIMLVSVTERTREIGIRLAIGARRRDIT
                     LQFLLESILISLCGCLIGLIIGVGGSWLGQRLFDLPVLVTPSSVLLALSVASSIGIFF
                     GLYPARRAARLNPIDALRQ"
BASE COUNT          784 a         1378 c         1383 g          753 t
ORIGIN      
        1 cgggtggccg cggcgcagtg ggggcaggtg ctggcacaac ggctgccaac gctcgatctg
       61 ggggtggcga tcgagcggca agagcggcac ggtggcctct ccacgtcgat cgatcagaat
      121 ctgctcagcc cgaatctgtc gctgagctat ctgctgttcg atttcggtgg ccggcaggcc
      181 cgtgacgagt cggcgcgggc gacgctgctg gcggccctcc acagccagaa cagcagcacg
      241 cagcggctga ttctggcggt tctgcaggcc tactaccagt tgattgccaa tgatgcggca
      301 gtgaccgccg cccgcgccaa tgagcagaca gccgaaaccg tgctgaaggc cgcgcaactg
      361 cgccatcaga tcggcagcgt cacccgggtc gatctgctgc aggcgcagag tgcctactcc
      421 cagacccggc tcaatcgcca gagcgccgag ggcaatgcac gcactgcccg cggggtgctg
      481 gccaacagca tcggtctcga tgccgatgtg ccgctcgccg tggtggcgcc gccacagccg
      541 aaacccgatc cgatcgccga agagaacatc catctgctga tcgaacaggc cagagggcag
      601 cgccccgatc tggccgccgc cgaagcgcag gtgagggcgg cactggccaa tcagcgtgct
      661 gtccgatctg ccgggctgcc cagcctgtcg ctgagtctcc agcagatcaa cagctatccg
      721 agtgtcacgc ccgacagccg tgacacccag gttggtctgc aactgtcgat gccgctcttc
      781 accggctttg ccgacagcta tcggttgcgc gccagccagg ccgaaatcga attgcagcag
      841 gccaggagcg agctgctcga caatcaggtg gcattggagg tgtggcagcg ctaccacgca
      901 ctgcaaacca gccgcgatac gctctcgacc accctcgacc tgctggcatc ggctgaagcc
      961 gctgcccaga tggcgttggg acagtaccgc agcggcgtcg gcacgctgct caatctgctg
     1021 acgctgcagg cccagctggc caacgcccgg ttgcagcaca ttcaggcaca atacaactgg
     1081 ttcagcagcc gcatggcgct ggcgcaggcg ctgggtcagt tgactccgac cagtgccagc
     1141 gagccggcca gtcaaccccc tcctgcatcc cgatgagcca cgcatgtcac gcaccaagct
     1201 tcttgtctca ctgatcaccg tgctgctgct cggcgctgct gcagtcggag gttatggcct
     1261 ctggcagaaa cgcacccagc aggcgcaagg cgagcagttc cggatcgagc agctcgaccg
     1321 gggcgagatc gtgcagatca tctcggccaa tggcacggtc aatccggtgg tgatggtcaa
     1381 cgtcggcacc caggtttccg gcacgctgca aaagatccat gtcgacttca acgcccaggt
     1441 gcgggaggga cagattcttg ccgaactgga cccgcggctg ctgcaggccc aggtcgatca
     1501 ggatgaggcc aatctggcca gcggccaggc caacctgaag ttgcagcagg ccaactggaa
     1561 gcggctgaag acactgtatg aacaggagta catctcgcgc gccgacttcg atgcggcgac
     1621 gcaggcgcgc gaggcggccg aggcgcaggt ggcgctgcaa caggcgcagt tgcgccgttc
     1681 gcggaccaat ctgagctaca ccatcatccg ttcgccggtc tccggcatcg tggtctcgcg
     1741 cctggtcgat gtcggccaga ccgttgcggc cagctttcag acgccggtgc tgttccagat
     1801 cgccaaggat ctggggcaga tgcagatcga caccagcgtc gccgaagccg acctgggcaa
     1861 tgtccacctc ggacaggagg tgagcttcta tgtcgatgcc tttcccgacc accgttttgt
     1921 cggcgaggtc aagcagattc gcctcaaccc gaccacgcag cagaatgtgg tcacctacaa
     1981 cgtggtggtg gcggtggaca acccggcagg caagctgtca cccggcatga ccgcctatgc
     2041 cagcctcatc gtcagccgca agccggatgt cgtgcgcttg cccaacgcgg cgttgcgctt
     2101 caggccaccg gcagaaaagg agcaggaacc acagggcagg gggggcagaa gcgccgccgg
     2161 ggcagtggtc tatcaattgc gggatggcaa gccggtggcg gtcccggtca agaccggcag
     2221 cagtgacggc cgctacaccg agctggtcga gggcgcactc accgcaggag agagcgtcat
     2281 cgtcggcctg aagcgccccg cagccgaatc cggcagcggc ggtggcacac gacggatgtt
     2341 ctgatgagtg ccctgatccg caccgaacgg ctcaccaaaa gttatgccgg acagaacggc
     2401 aaggcagccg tcgagatctt gcatggtatc gacttcagca tcgaagctgg cgagttcgtg
     2461 gccatcatgg ggcagtccgg ttcgggaaag tcgaccttga tgaatctgct cggctgtctc
     2521 gatgtggcca gcagcggcca ctactggctc gacggagagg cggtggatca actcagtccc
     2581 gatgcactgg ccctgctgcg caaccgcctg atcggctttg tatttcaagg cttcaatctg
     2641 ctgccgcgca gcaatctgct cgacaacgtt gccctgccgc tgctttatgc cggcagtgcg
     2701 cgccaggcac gacaccagcg cgccgaagag atgctggcgg cagtgggtct ggccggactg
     2761 ggacagcgtc tgcccagcca gttgtcgggt ggacagcagc agcgggtcgc catcgcccgt
     2821 gcgctggcca accatccccg cctgattctg gccgatgagc ccaccggcaa tctcgacagc
     2881 cacaccagtg acgagatcat ggcgctcttc tcccggctca accgcgagca gcggatcgcc
     2941 atcgtgctgg tgacccacga agccgatgtc gccgcctaca ccgaccggct gattcgcctg
     3001 cgcgatggcc gcatcctcga tgatggcccc acccaggcac tgctccagcg gtgaatggcc
     3061 gatgatcttc tggcccattt tcggcgaagc ctggaaggcg atgggtgcca accgcctgcg
     3121 caccttcctg accatgctgg ggatgatcat tggtgttgcc gccgtgattc tgatgctcgc
     3181 cttcggtcgt ggcgcgcagg agagcgtcaa caaatcgatt gccgacatgg gcaccaatct
     3241 gttgatgatt ctctcgggtt cgaccacctc cggcggtctg cgcatgggca tgggcagcac
     3301 gcccaccctc accatcgagg atgcccgagc gatcgccgaa ctgccgtcga tcgagcgtgc
     3361 ctctgcggtg atgagcggca ccgcgcaact ggtgcagggc ggcaacaact ggagcagcct
     3421 ggtctatggg gtcactccgg actacctcga ggtacgcaat tgggcgctgg cttcgggcaa
     3481 cagctttgac gaagccgcgc tgcgctctgc cacccaggtg gcgttgctgg ggcagaccgt
     3541 ggccgacaat ctttttcctg acggtgaaaa tccgttgggg aaaaccattc gcatcaagaa
     3601 catgcccttt ctggtgattg gtctgctcga ggtcaagggc cagagcatgg agggccgcaa
     3661 ccaggatgat gcggtggtga ttccggtcac caccgcacag cggcggctgt tcggtacccg
     3721 ctttcgcggc tcggtgcaat acatcatggc gcaggcggtg tcggcggagc tggccaagga
     3781 ggccgaacag gagatcaccc agctgctgag acagcgtcac caactgggcg acaatgaaca
     3841 gaatcgcaag ccggatgact tcaccttgcg caacctgtcg gagctggcgc agaccgccgc
     3901 cgtggccgcc cgcgcctttg cgctgctgtt gggggccatt gcctcgatct cgctgctggt
     3961 gggtggcatc ggcatcatga acatcatgct ggtgtcggtc accgagcgca cccgcgaaat
     4021 cggcatccgt cttgcgatcg gtgcgcggcg ccgtgacatc acgctgcaat tcctgctgga
     4081 gtcgatcctg atctcgctct gcggctgcct gattggcctg atcatcgggg tgggcggcag
     4141 ttggctgggg cagcgcctgt tcgatctgcc ggtgctggtc accccctcat cggtgctgct
     4201 ggcgctgtcg gtcgccagct cgatcggcat cttttttggc ctctatccgg cgcgacgtgc
     4261 ggcccggctc aatccgatcg atgcgttgag gcagtagg
//
LOCUS       DAORCQ010000141         4177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_16662_length_4177_cov_5.576419, whole genome shotgun sequence.
ACCESSION   DAORCQ010000141 DAORCQ010000000
VERSION     DAORCQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16662_length_4177_cov_5.576419"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   6..258
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6..42
                     /rpt_unit_seq="gtttctcccggccgaaaagccgggagcggattgaaac"
     gene            complement(270..977)
                     /locus_tag="PKI23_10865"
     CDS             complement(270..977)
                     /locus_tag="PKI23_10865"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNN37478.1"
                     /translation="MTSQTQIKLFSTLPHPCSYLPDEVATSLFVDPELPMTGRLYTVL
                     SRMGFRRSGAHVYRPNCRSCSACLSIRIPVQRFAPDRQQRRVWKKNDDVTLQLVESID
                     SALHYDLYQRYICQRHADGDMFPPTPEQFHSFLATACDFTRFIELRIGEQLIAVAVAD
                     LLDDGLSAVYTFFDPDLEPRSLGVLAVLWQIEYCRQRGLPYLYLGYWIQNCRKMRYKL
                     RYRPAELLIDQRWIQLN"
     gene            complement(987..1721)
                     /gene="aat"
                     /locus_tag="PKI23_10870"
     CDS             complement(987..1721)
                     /gene="aat"
                     /locus_tag="PKI23_10870"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNN37479.1"
                     /translation="MSVQAPALLDPGQIHFPPIEQALEEPNGLLAIGGDLSPERLLHA
                     YRHGIFPWYEAGQPILWWSPDPRAVIFPEHYRPSTSLRKRIRRGDYQVTTDQAFSAVV
                     AHCAAPRAGATGTWITREMHAAYCRLHRLGFAHSVESWQQGELVGGLYGVAIDRVFFG
                     ESMFSRRSDASKVAFAQLVDWLREWGYRLIDCQVANDHMISLGAEQIDRARFARLLHE
                     YCQRWQSCRPWARDAGAVADQPPANQ"
     gene            complement(1740..2693)
                     /gene="trxB"
                     /locus_tag="PKI23_10875"
     CDS             complement(1740..2693)
                     /gene="trxB"
                     /locus_tag="PKI23_10875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNN37480.1"
                     /translation="MTTVQHHPLIILGSGPAGLTAAIYAARANLAPVVISGLELGGQL
                     TTTTDVDNWPGDVEGLQGPALMARMQQHAERFETRIVMDRIIEADLSRRPFRLQGDSQ
                     AYSCDALIIATGASARYLGLPSEQLYRGRGVSACATCDGFFYRKKRVAVIGGGNTAIE
                     EALYLSNIAAEVVLVHRRDQLRGEKIMQQKLLDRAATGNIRIEWNQTLDEVLGDDSGV
                     TGMRLRSTLDGQCQQIDLHGIFIAIGHQPNTGIFSGQLEMEGGYIRIQGGGWGNATAT
                     SVPGVFAAGDVADSVYRQAITSAGFGCMAALDAERYLDGHG"
     gene            2865..3875
                     /locus_tag="PKI23_10880"
     CDS             2865..3875
                     /locus_tag="PKI23_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNN37481.1"
                     /translation="MSASRPIPTNLISGFLGAGKTTAILHLLRQKPADERWAVLVNEF
                     GEIGIDGAILAGEGAYVREVTGGCICCVAGLPLRVGLNLLIAQARPDRLLIEPTGLAH
                     PQQIGKMLTDEHYRSQLELRANLCLVDPRKLGDARYLDNDTFNAQLRLADLIVGTKSD
                     LCDAQDRQRFEQLLAALPTPRPAQEWVAHGQIDPAWLDLPRLERPSTTAVVLDAHHHH
                     HHGEDDAEPASIESVRLAVGQRFVRRENQGQGHYSCGWLFAPDTRFSLSLLMQLVSQL
                     TVLRCKAVVDTSQGRLVLNMESGALSVSPTRSEEAGRIEIIDSEPLDWDAIEQDLLLT
                     LQ"
     gene            3940..>4177
                     /locus_tag="PKI23_10885"
     CDS             3940..>4177
                     /locus_tag="PKI23_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37482.1"
                     /translation="MNARHTARPRLLKGRSALCLLLGGWLLLALQSGWTQTLELREAR
                     FLLSDAQQPPADDAAWQPQPLPDIWTQSRNGIGGV"
BASE COUNT          750 a         1335 c         1389 g          703 t
ORIGIN      
        1 ccgccgtttc tcccggccga aaagccggga gcggattgaa acttgctttt cgttatttta
       61 acggtgacgc ggctaaggtt tctcccggcc gaaaagccgg gagcggattg aaactctcct
      121 ttgagcacgc cttgcagcgc gactcatagt ttctcccggc cgaaaagccg ggagcggatt
      181 gaaacagcga aggcggaacg ttgcgcgccc ttgtgctcaa ggtttctccc ggccgaaaag
      241 ccgggagcgg attgaaaccg ggccgccgct cagttgagct ggatccagcg ctggtcgatc
      301 agcagttcgg ccggccgata gcgcaatttg tagcgcattt tccggcagtt ctgaatccag
      361 taacccaggt agagataggg cagcccgcgc tgacggcagt actcgatctg ccacagcacc
      421 gccagcacgc cgaggctgcg tggctccagg tcaggatcaa agaaggtgta gaccgccgac
      481 agtccatcat cgagcaggtc ggccaccgcc accgcgatca actgctcgcc gatgcgcagt
      541 tcgatgaagc gggtgaagtc gcaggcggtg gccagaaagg agtgaaactg ctcgggcgtg
      601 ggcgggaaca tgtcgccatc ggcatggcgc tggcagatgt agcgctggta gaggtcatag
      661 tggagggcgc tgtcgatcga ttcgaccagt tgcagggtga catcgtcgtt cttcttccag
      721 acccggcgct gctggcggtc gggcgcaaag cgttgcaccg gaatgcggat cgacaggcag
      781 gcgctgcaac tgcggcagtt ggggcggtag acatgggcgc cgctgcgacg aaaccccatg
      841 cgtgacagca cggtgtagag ccggccggtc atcggcagct ccggatcgac gaagagcgag
      901 gtggccacct catcgggcag gtagctgcat ggatggggca gcgtgctgaa gagcttgatc
      961 tgggtctgac tggtcatggc ctccactcac tggttcgcgg gcggttgatc ggcaaccgct
     1021 ccagcatcgc gcgcccatgg ccggcaactt tgccagcgct gacaatattc gtgcaacaac
     1081 cgtgcaaaac gtgcccggtc gatctgctcg gcacccaggc tgatcatgtg gtcattggcc
     1141 acctgacagt caatcagccg atagccccac tctcgcagcc agtcgaccag ctgggcaaag
     1201 gccaccttgg aggcatcgct gcggcgactg aacatcgatt cgccgaagaa gacccggtcg
     1261 atcgccacgc catagaggcc accgaccagt tcaccctgct gccagctctc caccgaatgg
     1321 gcaaagccga ggcgatgcag acggcagtag gcggcatgca tctcgcgggt gatccaggta
     1381 ccggtcgccc cggcgcgcgg ggccgcacag tgggccacca cggcgctgaa tgcctggtcg
     1441 gtggtgacct ggtaatcgcc acggcgaatc cgcttgcgca ggctggtcga ggggcggtag
     1501 tgctccggaa agatcaccgc acgcgggtcg ggcgaccacc agaggatcgg ctgcccggcc
     1561 tcataccagg gaaagatgcc gtgccgatag gcgtgcagca gccgctcggg ggagaggtca
     1621 ccgccaatcg ccagcaggcc gttgggctcc tccagcgcct gctcgatcgg cggaaagtgt
     1681 atctggccgg ggtcgagcag cgccggcgcc tgaacgctca tccgctgact ccgccgccat
     1741 cagccatggc cgtcgagata gcgctcggca tcgagtgccg ccatgcagcc aaagccggcc
     1801 gaggtgatgg cctgccggta gaccgagtcg gccacgtcac cggcggcaaa gacgccgggc
     1861 accgaggtgg ccgtggcgtt gccccaaccg ccgccctgaa tcctgatgta accaccctcc
     1921 atctccagtt gcccactgaa gattccggtg ttgggttggt gaccgatggc gatgaaaatg
     1981 ccatgcaggt cgatctgctg gcattggcca tccagcgtcg agcgcagccg catgccggtg
     2041 acgccgctgt catcgcccag cacttcgtcg agcgtctggt tccattcgat gcggatgttg
     2101 ccggtggcgg cacggtcgag cagcttctgc tgcatgatct tctcgccgcg cagctgatcg
     2161 cgccggtgca ccagcaccac ttcggcggcg atgttggaga ggtagagcgc ctcttcgatc
     2221 gcggtgttgc cgccaccgat caccgccacc cgcttcttgc ggtagaaaaa gccatcgcag
     2281 gtggcacagg cgctgacgcc acggccgcgg tagagctgtt cggagggcag gccgaggtag
     2341 cgggccgagg cgccggtggc gatgatcagc gcatcgcagc tgtaggcctg actgtcgccc
     2401 tgcaaccgga acgggcggcg gctgaggtcg gcctcgatga tgcggtccat cacgatccgt
     2461 gtctcaaacc gctcggcatg ttgctgcatc cgcgccatca gcgccggtcc ctgcaacccc
     2521 tcgacatcac caggccagtt gtcgacatcg gtggtggtgg tcaactgtcc gcccagctcc
     2581 aggccactga tgaccaccgg agcgaggttg gcgcgcgcgg catagatggc ggcggtcagg
     2641 ccggccgggc ctgaaccgag gatgatgagc ggatggtgct ggacggtggt catgaggcag
     2701 tgcggctcca cagttggatt tgggaggccg attatagcca tgcgccatga agagcgccca
     2761 gccgcgcttg ggcaggcctg ctgccgggcc gatcgccagc cgtcacgcgg acggctagaa
     2821 tctgcgcggc aattcactgc gccctcaccc tgcaaggagt cccgatgtcg gcatcccggc
     2881 ccattcccac caacctgatc agtggctttc tcggcgcggg caagaccacc gcgattctgc
     2941 acctgctcag acagaagccg gccgatgagc gctgggcggt gctggtcaac gagttcggcg
     3001 agatcggcat cgatggcgcg attctggccg gcgagggcgc ctatgtgcgt gaggtgaccg
     3061 gcggctgcat ctgctgtgtg gccgggttgc cgctgcgggt cggactcaat ttgctgatcg
     3121 cccaagcccg gcccgaccgg ctgctgatcg agccgaccgg cctcgcccat ccgcagcaga
     3181 tcggcaagat gctgaccgac gagcactacc ggtcacagct cgagctgcgc gccaacctct
     3241 gtctggtcga tccacgcaag ctgggcgatg cgcgttacct cgacaatgac accttcaacg
     3301 cgcagttgcg gctggccgat ctcatcgtcg gcaccaagag cgacctgtgc gatgcgcagg
     3361 accggcagcg cttcgaacag ctgctggccg cgctgccgac cccgcggccg gcgcaggagt
     3421 gggtggccca tggccagatc gatcccgcct ggctcgacct gccgcggctg gagcggccct
     3481 ccaccaccgc ggtggtgctc gatgcgcacc accatcacca ccacggcgaa gacgacgccg
     3541 aaccggccag catcgaatcg gtgcggctgg ctgtcggtca gcgcttcgtc cggcgcgaga
     3601 accagggaca gggacactac agttgcggct ggctgtttgc ccctgacacc cgcttttcgc
     3661 tgtcgctgct gatgcaattg gtgtcacaac tgacggtgct gcgctgcaag gcggtggtcg
     3721 acaccagcca ggggcgtctg gtgctgaaca tggagagcgg cgcgctgtcg gtgtcaccga
     3781 cccgcagcga ggaggccggt cgcatcgaga tcatcgacag cgaaccgctc gactgggatg
     3841 cgatcgaaca ggatctgctg ctgacgctgc aatagccggg actgccggct atgctcaagc
     3901 gatgagccat ctgatcgaca acgcggtcgt cacgaccgca tgaacgccag acacaccgca
     3961 cgcccacgcc ttctgaaagg acggtctgcc ctctgtctgc tgctgggcgg ctggctgctg
     4021 ctggccctgc agtcgggctg gacgcagacg ctggagctgc gcgaggctcg ttttctgctc
     4081 agtgatgcgc agcagcctcc agcggacgat gccgcctggc aaccgcagcc actgcccgac
     4141 atctggaccc agagccgcaa tggcatcggc ggggtcg
//
LOCUS       DAORCQ010000142         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_16766_length_4161_cov_5.532879, whole genome shotgun sequence.
ACCESSION   DAORCQ010000142 DAORCQ010000000
VERSION     DAORCQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16766_length_4161_cov_5.532879"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /gene="pepN"
                     /locus_tag="PKI23_10890"
     CDS             complement(<1..567)
                     /gene="pepN"
                     /locus_tag="PKI23_10890"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNN37483.1"
                     /translation="MNHPAPQPVLLSDYRPPAFLIDHTRLHFDLDPAATEVTAELTMR
                     RNPAAQATDRLRLDGVELELCSVTLEGQPLSPQQYRQDAESLTISGVPEAFTLRLVTR
                     IHPADNSALEGLYLSQGRLCTQCEAEGFRKITYYLDRPDVMSRFSTTLVGDAERFPVL
                     LSNGNPVAHGSLGKRSASPTRVVEKRDIT"
     gene            complement(564..836)
                     /locus_tag="PKI23_10895"
     CDS             complement(564..836)
                     /locus_tag="PKI23_10895"
                     /inference="COORDINATES: protein motif:HMM:NF018698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HNN37484.1"
                     /translation="MNLPQLVDTLDEATYQRLKQSIETGRWPDGRLLSSEQRENSLLL
                     LIAYETRHLPTEARTGYLPPKSDCDPTPGDSAAPLVIRHRDETHSR"
     gene            complement(840..1706)
                     /locus_tag="PKI23_10900"
     CDS             complement(840..1706)
                     /locus_tag="PKI23_10900"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNN37485.1"
                     /translation="MHKALEISHPANLRGFSRLLHSHGIEHRIVEERGHQVLWVSEAG
                     QIEQATALYRRFLADPSLQHRAAPAVVANDSEWWLAWLRAPVTLTLVATTVGVTGLLW
                     LTDSTTLLGWLTFYDLTQIGPYAILNPAVASQPWRLLTPIFLHFGLLHLVFNMLWLAE
                     LGRRIEWAQGGKVLLLLVVVLGLISNCAQAWVARESLFGGMSGVIFGLFGYCWLWERF
                     RKTRLFALPRGVIGFLLIWLALGYSNILTIAGFGSIANTAHASGLLAGLLVAAIALIA
                     TASPPRQRREKE"
     gene            complement(1699..2676)
                     /locus_tag="PKI23_10905"
     CDS             complement(1699..2676)
                     /locus_tag="PKI23_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNN37486.1"
                     /translation="MADAPGGYDLIGDVHGCALTLFHLLEKLGYQKRAGVYRHPSRQL
                     ILTGDLIDRGPRIREALHQVRDMVEAGAAQMVMGNHEYHALCHGTPAKAGSPHPYLRE
                     HSPRHQAVIEATLEQFANHHADWHAFLDWFLTLPLFLELPGCRVVHACWDHALIDQFR
                     QQHGGNRLTADLLQQSVARNSFAYRLVNRLLHGLMLPLPDGDSITGHDGIARSHFRAK
                     FWEEDPASYADLLFQPDLLPVEVAQRMISQEHRARLLHYRADEPPLFIGHYWRTGVPE
                     TIRPNLACLDYSAVKSGKLVCYRFDGETRLDSAKFVWQTMELDQPRRHA"
     gene            complement(2692..3576)
                     /locus_tag="PKI23_10910"
     CDS             complement(2692..3576)
                     /locus_tag="PKI23_10910"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNN37487.1"
                     /translation="MEQFRNVGLIGRLESPGVIETLKTLKEFLVAEGYRVILEESTAA
                     EFIDHGLQVSHLRLLGEICDLAIVVGGDGSILGAARALARHKVPVLGINRGRLGFLTD
                     ISPDEVPAKITDVLAGKYIMESRFLLDAQLQRNGEPVNSGRALNDVVLHPGQATRMIE
                     FELYIEGQFVYSQRSDGLIVATPTGSTAYALSAGGPIMHPTLDAIALVPMFAHTLSSR
                     PIVVGGSSEIKIVLGVENRFNLQISCDGQDHLIAAPGDVLYVQKYPHKLRLIHPLSHN
                     FYEICRSKLGWGNLLERT"
     gene            complement(3688..>4161)
                     /locus_tag="PKI23_10915"
     CDS             complement(3688..>4161)
                     /locus_tag="PKI23_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HNN37488.1"
                     /translation="GNTTRLAALDEAFRLQEVELCYAREHRNRLEAELAKLQQERADW
                     AGQSADGLLARLQALGVALIAYHPGAGHFNLALATIPDYLANPQAQAAQRCQVSETHY
                     RLWLDHYQNPRCNAVQAGGKRCEQPVAPVQAPAHFTTGLSDRCPLHRHDRLRVQG"
BASE COUNT          755 a         1250 c         1405 g          751 t
ORIGIN      
        1 cgtgatgtcg cgcttctcca ccaccctggt tggtgatgca gaacgttttc cgaggctgcc
       61 atgagccacc ggattgccgt tcgacagcag caccggaaaa cgttctgcat caccaaccag
      121 ggtggtggag aagcgcgaca tcacgtcggg gcggtcgagg tagtaggtga tcttgcgaaa
      181 gccctcggcc tcgcactggg tgcagaggcg cccttgcgag aggtagagcc cttccagcgc
      241 gctgttgtcg gcggggtgga tgcgggtcac cagccgcagt gtgaatgcct ccggcacgcc
      301 gctgatcgtc agtgactcgg catcctgacg atactgctgc ggtgacagtg gttgaccctc
      361 cagcgtcacc gaacagagtt cgagctcgac accatcgagc cgcagccggt cggtggcctg
      421 cgccgccgga ttgcggcgca tcgtcaattc ggcagtgacc tcggtggcgg ccggatcgag
      481 atcgaaatgc agccgggtat gatcgatcaa aaacgcgggc gggcggtagt cgctcagcag
      541 cacgggttga ggggcgggat ggttcatctg ctgtgggtct cgtcgcggtg gcggatgaca
      601 aggggtgcgg cgctgtcgcc cggagtcggg tcgcagtccg atttcggcgg aaggtagccg
      661 gtgcgtgcct ctgtgggcag gtggcgggtc tcataggcga tcagcagcaa cagactgttc
      721 tcgcgctgtt cgctgctcag cagccggcca tcgggccagc gaccggtctc gatcgactgt
      781 ttcagtcgct gataggtggc ttcatcgagc gtgtcgacca gttgcggcag attcaccgat
      841 cactccttct ctctgcgttg tcgagggggg cttgccgttg cgatcagcgc aatggccgcc
      901 accagcagtc cggccagcag gccggaggca tgggcggtgt tggcaatcga accgaaaccg
      961 gcaatggtca gaatgttgct gtagccgagt gccagccaga tcagcaaaaa gccgatgacg
     1021 ccccggggca gggcgaacag ccgggtcttg cggaaccgct cccacagcca gcagtagccg
     1081 aacagaccga agatcacccc ggacatgccg ccgaagagac tctcccgcgc gacccaggcc
     1141 tgagcgcagt tggagatcag cccgagcacc accaccagca gcagcagaac cttgcccccc
     1201 tgtgcccact cgatgcgccg tcccaactcg gccagccaga gcatgttgaa gaccaggtgc
     1261 aaaagcccga agtgcagaaa gatcggagtc agcaggcgcc agggttggct ggccacggct
     1321 ggattcagaa tggcgtaggg gccgatctgg gtcaggtcgt aaaaggtgag ccagcccagc
     1381 aaagtggtgc tgtcggtgag ccagagcaga ccggtcacac cgactgtggt ggcaaccagt
     1441 gtcagcgtca ccggggctcg cagccaggcc agccaccact cggaatcgtt ggctacgaca
     1501 gcgggtgcgg cccgatgctg gaggctgggg tcggccagaa aacggcgata gagcgcagtg
     1561 gcctgttcga tctggccggc ctcgctgacc cacagcacct gatggccgcg ctcctcgacg
     1621 atgcggtgtt cgatgccatg gctgtgcagc aatcggctga agccgcggag gttggcagga
     1681 tggctgattt cgagcgcctt atgcatgtct gcgtggctga tcgagttcca tcgtctgcca
     1741 gacgaacttg gccgaatcga gccgggtttc gccatcgaag cgatagcaga ccagcttgcc
     1801 tgatttgact gcgctgtagt cgaggcaggc gaggttggga cggatggtct ccggcacgcc
     1861 ggtgcgccag tagtggccga tgaacagtgg cggttcgtcg gcgcggtagt gcagcagccg
     1921 ggcgcggtgc tcctgcgaaa tcatgcgctg cgccacctcg accggcagca gatcgggctg
     1981 gaacagcaga tcggcatagc tggccggatc ctcctcccag aatttggccc ggaagtggct
     2041 gcgggcgatg ccatcatggc cggtgatgct gtcgccatcc ggcagcggca gcatcagccc
     2101 atgcaacagg cggttgacca ggcggtaggc gaagctgttg cgcgccaccg actgctgcag
     2161 caggtcggcg gtcagtcggt tgccaccatg ttgctgccgg aattgatcga tcagcgcatg
     2221 gtcccagcag gcatgcacga cgcggcagcc gggcagctcc agaaacagcg gcagcgtcag
     2281 gaaccagtcg agaaaggcgt gccagtcggc atggtggttg gcgaactgct ccagcgtcgc
     2341 ctcgatgacc gcctggtggc gcgggctgtg ttcgcgcagg tatggatgcg gactgcccgc
     2401 cttggccggc gtgccatgac agagcgcgtg gtactcatgg ttgcccatca ccatctgcgc
     2461 ggcaccggcc tcgaccatgt cgcgcacttg gtgcagcgcc tcgcggatgc gcggaccgcg
     2521 gtcgatcaga tcgccggtca gaatcagctg gcggctgggg tggcggtaga cgccggcgcg
     2581 cttttggtag cccagcttct ccagcaggtg gaacagggtc agcgcgcagc catgcacatc
     2641 gccgatcagg tcgtagccac cgggagcgtc agccatggcg gcaccgatga ttcatgtgcg
     2701 ctccagcaga ttgccccagc ccagcttgct gcggcagatt tcgtagaaat tgtggctgag
     2761 cgggtggatc aggcgcagct tgtgcggata cttctgcaca tagagcacat cgccaggcgc
     2821 cgcgatcagg tggtcctggc catcgcaact gatctgcagg ttgaagcggt tctccacccc
     2881 cagaacgatc ttgatctcgc ttgacccacc caccacgatc ggccggctgc tgagggtgtg
     2941 ggcgaacatc ggcaccagcg cgatggcgtc gagtgtcgga tgcatgatcg gcccacccgc
     3001 cgacagggcg taggcggtcg aaccggttgg cgtggcgacg atcagcccat ccgaacgctg
     3061 gctgtagacg aactggccct cgatgtagag ctcgaactcg atcattcgtg tcgcctgccc
     3121 cgggtggagc acgacatcgt tcagcgcgcg gccgctgttg accggttcgc cattgcgctg
     3181 caactgggca tcgagcagaa agcggctctc catgatgtac ttgccggcca gtacatcggt
     3241 gattttggca ggcacttcgt cgggagagat gtcggtgagg aatcccagtc ggccccggtt
     3301 gatgcccaat accggcacct tgtgtcgggc cagtgcgcgg gcggcgccga ggatgctgcc
     3361 atctcccccc accacgatcg ccagatcgca gatttcgccc agcaggcgca gatggctcac
     3421 ctgcaggcca tggtcgatga attcggcggc ggtgctctct tcaaggatga ctcgatagcc
     3481 ttcagcgacc agaaactcct tgagcgtttt cagggtttcg atcacgccgg ggctttcgag
     3541 cctgccaatc agcccgacat tgcgaaactg ctccattgag catccatcat gacaaaaaga
     3601 gtggctgatt atggtgccgc cggccgtttc cgtccagaaa cagtgctcga cgcagggcca
     3661 cgcgcggggc gtggatggtg tgtgagccta accctgcacg cgcaggcgat catgccgatg
     3721 gagcgggcag cgatcactga ggccggtggt gaaatgggcg ggtgcctgca caggcgccac
     3781 gggctgctcg cagcgcttgc caccggcctg aactgcgttg cagcgtggat tctggtaatg
     3841 atcgagccag agtcgatagt gtgtttcgct cacctgacaa cgctgtgctg cctgggcctg
     3901 cgggttggcc agatagtcag gaatcgtggc gagggccaga ttgaagtggc cagcgcctgg
     3961 atggtaggcg atcagggcga cccccagtgc ctgcagtctg gccagcaaac catcggcgct
     4021 ttgcccggcc cagtcagcgc gctcctgctg caacttggcc agttcggctt cgagacggtt
     4081 gcggtgctcg cgcgcataac agagctccac ctcctggagt ctgaaggctt catcgagtgc
     4141 ggcgagtcga gtcgtgttcc c
//
LOCUS       DAORCQ010000143         4088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_17240_length_4088_cov_6.116787, whole genome shotgun sequence.
ACCESSION   DAORCQ010000143 DAORCQ010000000
VERSION     DAORCQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17240_length_4088_cov_6.116787"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKI23_10920"
     CDS             <1..602
                     /locus_tag="PKI23_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37489.1"
                     /translation="LTPIQFLQLLHLVLETASPKDAAMSQPVIRLSSTHKRLIYLVFA
                     LLWMSGALWLLSHYFFAIEGDFGPTPHPLEKWALRLHGFTAMLALIAVGSLATHHMRL
                     AWSRKQNHRSGMAMFTLNAWLAATGYALYYFATDDNAAWLPLLHWVVGLALPPALVAH
                     VVVGRRRTSHGRLQPKKQPRVAGRPGSTSASRIGPKSNL"
     gene            881..1789
                     /locus_tag="PKI23_10925"
     CDS             881..1789
                     /locus_tag="PKI23_10925"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNN37490.1"
                     /translation="MQRISRLTCAPLVSFALCCALLSLPVMAAERQCEVVEGTIVCQT
                     QKDLTAGLDPTAKGLVVYSEMDNRNGATLGFGGRGYVDGVSELEATIRTADGSEHVRR
                     VHMRFLERENSSDRRMMIMENPDDQKGMALLTISNPTGLDDQWLFDTTQKQVRRVSQN
                     HIGTPFAGTDLNFEDLSLQNIDRYDYGFLGEEKLSGHDCYIVVRRPKYPYSSNGKLVT
                     WVDKAHYYPVKIDYYDRHDTLVKTLTLFDYQQYDDRFWRAGRMELVNHLTAVRTTLQW
                     SGYRFNVGLSEHDFDLNALQRPRTLR"
     gene            1812..3137
                     /locus_tag="PKI23_10930"
     CDS             1812..3137
                     /locus_tag="PKI23_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNN37491.1"
                     /translation="MADPQYLLLFQGELAPGCRRREVLDRLTQLTNRDADSLIDQLFA
                     IKPLIIKRGGDLAFLEHFRQTLITAGLSIDLRQDDAKSAEIDEIDFVFAHYAPIEEAA
                     PQLSQAVLMQNPAAPRRQLGRHALLFEGQLADGLNQRQVIRNLASLCNCSERRVSDEL
                     FSVVPLLLATADQRVELEQALEEYQRAGLLLSVVKTERCDETLPLAALSIRHDRPEVT
                     LPHLKRWPFPALVGTFCLSISLLWLQGMLTQPPKPVTPSPPSSRLEIVLNAPPDPVVK
                     PAILPSLAEAVAKAAATPAPAMAVPKPPPPAARARRVATSPPAPPPEQAAPTASEPPP
                     AAPVTTGIDIARLSEAYDNRVRLWLAEHQSKQSTQGSIGAGRVTLAVELNREGRLLSG
                     RVSESSGHAALDAQALADLKAASPFPAMPVELTAATHALTIRILYRETQ"
     gene            3279..>4088
                     /locus_tag="PKI23_10935"
     CDS             3279..>4088
                     /locus_tag="PKI23_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37492.1"
                     /translation="MVQSTIDASSEKYLRGKYAIVGVGETGYTRGSGRTTRSLGTHAI
                     RNAIVDAGLKPSDIDGMLSYGFNDSTPSPTLAGDLGIRLNFYMDVIGGGSSIEALIGI
                     AIGVIEAGMCKTVAIFRAMNGFSQVRIGGTNRQGTVAPLGGEDLFSRGNGWMSAGQMF
                     APSFMRHMHEYGTTPEQVAMVKVIHAENASNNPKAYYKQRVTVDDVLSSRMIVKPLHL
                     LDCCVETDNGTAIIVTSIDRARDCRHTPAKILSVVGRCCKPRADNHVQHGPI"
BASE COUNT          811 a         1357 c         1196 g          724 t
ORIGIN      
        1 acctgactcc aattcaattt cttcaactgt tgcacttggt tcttgaaacc gccagcccaa
       61 aagacgcagc catgagccag ccggtcatcc gcctttccag cacgcacaag cgactgatct
      121 acctggtctt tgcgctgctg tggatgagcg gtgcgctctg gctgctgtcg cactacttct
      181 tcgccatcga aggcgacttc ggtccaacgc cgcacccgct ggaaaagtgg gcgttgcgcc
      241 tgcatggctt caccgcgatg ctcgcgctga tcgccgtcgg ctcgctcgca acccatcaca
      301 tgcgactggc ttggagccgc aagcagaatc accgcagcgg aatggcgatg ttcaccctga
      361 acgcctggct cgccgccacc ggttatgcgc tgtactactt cgccaccgac gacaacgccg
      421 cctggctgcc gctgctgcac tgggtcgtcg gtctggcact gccgcccgcg ctggttgcgc
      481 atgtcgttgt cggtcgccgc cgcacttccc atggacgact ccagccaaag aaacagcctc
      541 gcgtcgccgg tcgtcctggc agcaccagcg cctcccgcat cggcccgaaa agcaacctgt
      601 gaacgcctct tccatcacgt ccgggcattc gaccggttcc aactcgtagc catcgagagg
      661 gtgcgtcggc ctgacaggcg accaaatggc atgaggacgc atggactggg cttcacggcg
      721 ggcgagaaag caagccgctg tgcacgccaa agtctgaagc gaaaccggcc attgacgctg
      781 tccgcaccac gtcgacaagc cgctgtgatc gacctatgct ttcatggcaa cgtcgacctc
      841 tcccccatct cgacccgacc gatcgataaa ggattgaaca atgcagcgga tctcccggct
      901 gacctgcgcc ccactcgtct cattcgcact ctgctgtgcg ctgctgtcgc tgccggtgat
      961 ggcggctgag cggcagtgcg aagtggtcga aggcaccatc gtctgtcaga cgcagaagga
     1021 tctgaccgcc gggctcgatc cgaccgccaa gggtctggtg gtctactccg agatggacaa
     1081 ccgcaatggc gcaacgctgg ggtttggtgg ccgcggctat gtcgatggcg tctccgagct
     1141 cgaggccacc atccgcacgg ctgacggcag cgagcatgta agacgggtcc acatgcggtt
     1201 tctggagcgt gagaacagca gtgaccggcg catgatgatc atggagaacc ccgatgatca
     1261 gaaaggcatg gcgctgctga ccatctccaa tcccaccgga ctggatgacc agtggctgtt
     1321 cgacaccacg caaaaacagg tccggcgggt cagtcagaac cacatcggca cacccttcgc
     1381 cggcaccgat ctcaacttcg aagatctgag cctgcagaac atcgaccgtt atgactacgg
     1441 ctttcttggc gaggagaagc tctccggaca tgactgttac atcgtggtgc gtcgcccaaa
     1501 atacccctat tccagcaacg gcaagctggt cacctgggtc gacaaggcgc actactatcc
     1561 ggtcaagatc gactactacg accgccatga caccctggtc aagactctga cgctgttcga
     1621 ctaccagcag tacgacgacc gcttctggcg tgccgggcgc atggagctgg tgaaccatct
     1681 gaccgccgtg cgcaccacgc tgcagtggag cggctatcgc ttcaacgtcg ggctctccga
     1741 gcacgacttc gacctcaacg cactgcaacg gccgcgcacc ttgcgctgac tggagcgttt
     1801 cgtcaccggt gatggccgat cctcaatatc tgctgctgtt ccagggtgag ctggcaccgg
     1861 gctgtcgacg gcgcgaggtg ctcgatcgac tcacccaact gaccaaccgc gatgccgaca
     1921 gcctgatcga ccagctgttc gccatcaaac cgctgatcat caagcgcggt ggcgaccttg
     1981 cctttcttga gcactttcgc cagaccctga tcacagcggg gctgtcgatc gatctgcggc
     2041 aggacgatgc aaagagtgcc gagatcgacg agatcgactt cgtctttgcc cactacgcac
     2101 cgatcgagga ggctgcgccg cagctgagtc aggcggtgct gatgcagaac ccggctgcac
     2161 cccggaggca acttggacgt catgccctgc tgttcgaggg gcaactggcc gacggcctga
     2221 accagcgaca ggtgatccgc aatctcgcca gcctgtgcaa ctgcagtgag cggcgggtca
     2281 gcgatgagct cttctcggtg gtcccgctgc tgctcgccac ggccgatcag cgcgttgaac
     2341 tggagcaggc gctggaagag taccagcgtg ccgggctgct gctttcggtc gtgaaaacag
     2401 aacgttgcga tgagaccttg ccgctggccg cgctgtcgat ccgccatgat cgacctgagg
     2461 tgacgcttcc ccatttaaaa cgctggccat tcccggcgct ggtgggcacc ttctgcctgt
     2521 cgatcagtct gctgtggctg caggggatgt tgacccaacc acccaaaccg gtgaccccat
     2581 cgccgccctc gtcccgtctg gagattgtgc tgaatgcacc gcccgatccg gtggtgaagc
     2641 cagccattct gcccagcctc gccgaagccg ttgccaaggc cgcagccacg cccgcgcctg
     2701 cgatggcggt accgaaaccc ccaccgcccg ccgcaagagc caggcgcgtg gcaacgtcac
     2761 cacctgctcc accacccgag caggccgcgc ccaccgcaag cgaaccgccg ccggccgcac
     2821 ccgttaccac gggcatcgac attgcccgat tgagcgaagc ctatgacaac cgcgtccgat
     2881 tgtggcttgc cgagcatcag agcaagcaga gcacccaagg cagtatcggt gcgggccggg
     2941 tgacactggc tgtcgagctg aaccgtgaag gaagattgct gtcagggcgg gtcagtgaat
     3001 caagcggcca tgccgcgctc gatgcgcagg cgttggccga cctcaaggcg gccagcccgt
     3061 ttccggccat gccggtggag ctcacggcag ccacccatgc cctgaccatc cgaattctct
     3121 atcgcgagac ccagtgacga ggcgccacga atggcagcct gtcccatggc tggacatcag
     3181 ccggccattg caaatttttt gtgccatgtc gccatgacaa accgggcatc atcacggtaa
     3241 gctgcgccga caataatcac acaaggagag gagagaccat ggtgcaatcc accatcgacg
     3301 ccagcagcga aaaatacctg cgtggcaaat atgcgatcgt tggcgtgggc gagaccggtt
     3361 acacccgcgg ctccggccgc accacccgct cgctgggcac ccatgcaatc agaaatgcca
     3421 tcgtcgatgc cgggctcaag ccgagcgaca tcgacggcat gctctcgtat ggattcaacg
     3481 actcgacccc ctcccccacg ctggccggag atctgggcat ccggctcaac ttctacatgg
     3541 atgtgatcgg tggcggttcc agcatcgagg cgctgatcgg cattgccatc ggcgtgatcg
     3601 aggccggcat gtgcaagacg gtggcgatct tccgcgccat gaatggcttc agccaggtgc
     3661 gcatcggtgg caccaaccgg caaggcacgg tggcgccact cgggggtgaa gatctgttca
     3721 gccgcggcaa tggctggatg agcgccgggc agatgttcgc cccttcgttc atgcgccaca
     3781 tgcatgaata tggcaccacg cccgaacagg tggcgatggt caaggtgatc cacgccgaaa
     3841 atgccagcaa caaccccaag gcctactaca agcagcgggt gacggtggat gatgtgctgt
     3901 cgagccggat gatcgtcaag ccgctgcatc tgctcgactg ctgtgtcgag accgacaacg
     3961 gcaccgccat catcgtcacc agcatcgacc gcgcccgcga ctgccgtcac accccggcca
     4021 agatcctctc ggtggtcggc cgctgctgca aaccgcgcgc tgacaaccac gtccagcacg
     4081 gaccgatc
//
LOCUS       DAORCQ010000144         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_17502_length_4052_cov_5.669252, whole genome shotgun sequence.
ACCESSION   DAORCQ010000144 DAORCQ010000000
VERSION     DAORCQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17502_length_4052_cov_5.669252"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKI23_10940"
     CDS             complement(<1..1106)
                     /locus_tag="PKI23_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNN37493.1"
                     /translation="MSSEPRDSTPFIQALRDIVGPSGLLTGEDSVRIKGGLVRPGKAP
                     PPHILLRPASTEEVSAVLRLCNDAGYQVVPQGGMTGLVEGAEIAAGQVALSLERMSAI
                     EEIDTLSGTLLAQAGVPLQAAQEAAEAAGWMLPLDLGGRGSATLGGNVGTNAGGNRVI
                     RYGMTRAMVMGLEVVLADGTILTSLKKILKDNSGYDLKQLFIGAEGTLGVVTRILLRL
                     TPKPASQETALVALNDFASVTALLNHAKRQLDGTLSAFEVMWNGFYSAVTTPPAPHQP
                     PLPGHFPFYVLIEALGSDPQQDHPRFERAMSAALESGLISDAVVAKSEAERHRLWALR
                     DDVAQLGRYYPLTGFDISLPLEAMEAYLSAVEQRL"
     gene            complement(1178..1372)
                     /locus_tag="PKI23_10945"
     CDS             complement(1178..1372)
                     /locus_tag="PKI23_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37494.1"
                     /translation="MAEIISFSKQKKARRAALAKGRTLCANGFHRWKISKDQQFDVTA
                     GKLITVWHCERCDAVKNSAL"
     gene            1506..3335
                     /gene="dsbD"
                     /locus_tag="PKI23_10950"
     CDS             1506..3335
                     /gene="dsbD"
                     /locus_tag="PKI23_10950"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNN37495.1"
                     /translation="MRLLTLLLFTLGIVLGAVRGIQAEPTPPLTSPSATKLQSLLQSD
                     SGTPHFLPVNEAFRPRLRQSAGELQIDWQVAAGYYLYRDRFKFRLIAPAGVTLGTPRF
                     PDAVEKEDELFGKVAVFDHDFTLTLPTSVTSDAEVLLEVEFQGCAEAGLCYPPERLQL
                     GSATPPAAAGNTTTGAANSSALLAQKLASARFSVVLGLFFLAGIGLSFTPCVLPMIPI
                     LSSIVVGREQHPTRMQALLLTLLYVVSMALTLAVAGALTGLFGARLNLQAYLQWPPLL
                     VGFALLFVLLSLSMFGLYELQLPAALRNRFDQLNRQMSHGHLLGAMAMGALSTLVISP
                     CVSAPLAGALVYISTTQDAWLGGAALFALGLGMGVPLLLLATLGNHLLPKAGAWMERV
                     KSFFGLLLLGVAVWLLERLLPGPVTLLLWAALLLGGALLLGALEFTAHPSGWHKLGQT
                     LGLLLLAWAVALVLGAAAGGSDPLRPLHGLGIRQGQAAAVEHPSFTDITDLPGLDRQL
                     TLAAGRITLVDVYADWCIACKVLEREVFPTVTTQLAPLHRLRADITDYQAPQRALLDR
                     YRIVGPPTLLFLDAKGQELPDSRIVGEIDATQLASHLDRLLSR"
     gene            3471..3926
                     /gene="aroQ"
                     /locus_tag="PKI23_10955"
     CDS             3471..3926
                     /gene="aroQ"
                     /locus_tag="PKI23_10955"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008078187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNN37496.1"
                     /translation="MAKLLLLNGPNLNMLGRREPGHYGRQSLDAITDALVREAEARGH
                     TLCCFQSNAEHALIDRIHGALEDGTDFILFNPAAFTHSSVALRDALLAVAIPFIELHL
                     SNVHAREPFRHHSYFSDIAVGVISGFGADSYRLALLAAIERLATPKPSP"
     gene            3955..>4052
                     /locus_tag="PKI23_10960"
     CDS             3955..>4052
                     /locus_tag="PKI23_10960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937213.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNN37497.1"
                     /translation="MDIRKVKKLIELVEESGIEELEIKEGEESVRI"
BASE COUNT          628 a         1401 c         1276 g          747 t
ORIGIN      
        1 agccgctgtt cgactgcgct caggtaggcc tccatcgcct ccagcggcag gctgatgtca
       61 aagccggtga gcgggtagta gcggccaagc tgggcgacgt cgtcacgcag cgcccagagt
      121 cggtgccgtt cggcctccga ctttgccacc accgcatcgc tgatcagccc cgactccagt
      181 gccgcgctca tcgcccgctc gaagcgcgga tggtcctgct gcggatcgct gccgagcgcc
      241 tcgatcagca catagaacgg aaaatggccc ggcagcggcg gctggtgcgg tgccggcggc
      301 gtggtgacgg cgctgtagaa gccgttccac atcacctcga aggcgctcag cgtgccatcg
      361 agctgccgtt tggcatggtt cagcagcgcg gtgaccgagg cgaagtcgtt cagtgcgacc
      421 agggcggtct cctggctggc cggcttcggg gtgagtcgca gcaggatgcg ggtcaccacg
      481 cccagcgtgc cttcggcgcc gatgaagagc tgcttgaggt cgtagccgct gttgtccttg
      541 aggattttct tcagcgaagt gaggatggtg ccatcggcca gcaccacttc gagccccatc
      601 accatcgccc gggtcatgcc gtagcggatc acccggttgc cgccagcgtt ggtgccgaca
      661 ttgccgccga gggtggcgct gccgcgcccg ccgaggtcga gcggcaacat ccagccggcc
      721 gcttcggccg cctcctgcgc cgcctgcagc ggcacgccgg cctgcgccag cagggttccg
      781 ctcagggtgt cgatctcctc gatcgcgctc atgcgctcca gcgacagcgc cacctgcccg
      841 gcggcgatct ccgccccctc caccaggccg gtcatgccgc cctgtggcac cacctggtag
      901 ccggcgtcat tgcacagccg cagcacagcc gagacctcct ccgtgctggc cgggcgcagc
      961 agaatgtgtg gcggcggcgc cttgcccggc cgcaccagcc cacccttgat gcgcaccgaa
     1021 tcctcgccgg tgagcaggcc ggatggcccg acgatgtcgc gcaatgcctg aatgaagggg
     1081 gtggagtccc gaggctctga actcatgcgc tgctccatgc ggccacggcc gggtcgttgt
     1141 gatggttgca ctgcgccgga atggccgacg cggcgggtca cagggcgctg tttttcaccg
     1201 cgtcgcagcg ttcacagtgc cacaccgtga tcagcttgcc ggcggtgacg tcgaactgct
     1261 gatccttgct gatcttccag cgatgaaagc cgttcgcgca gagcgttctg cccttggcca
     1321 gcgcggcacg ccgtgctttt ttctgtttgc tgaaggagat gatctctgcc atcgctgcca
     1381 ccgctgatcg accccgattg aatcacccac ccaaagtgca gtcaaggcgc gctcttttgc
     1441 tacaatcgcc ggcgtcagcc gaacccggcc tgtcttcgcc tcacggaaaa aatcccttgc
     1501 cattcatgcg cctgctcacc ctgctgctgt tcacactcgg cattgtgctg ggtgcggtgc
     1561 gcggcattca ggccgagcca acaccgccgc tgacctcgcc ctcggcgacg aaactgcaat
     1621 cgctgctgca aagtgacagc ggcacgccac acttcctgcc ggtcaacgag gcctttcggc
     1681 cccggttgcg gcagagcgca ggcgaattgc agatcgattg gcaggtggcc gctggctact
     1741 acctctaccg cgaccgcttc aagttccggc tgatcgcccc ggcgggcgtc accctcggca
     1801 cgccgcgctt tccggacgct gtagagaaag aggatgagct gttcggcaag gtggcggtgt
     1861 tcgaccacga cttcactctg acgctgccca cctctgtgac cagcgacgcc gaggtgctgc
     1921 tcgaagtgga gttccagggc tgcgccgaag ccgggctctg ttatcccccg gagcggctgc
     1981 aactcggttc ggccacgccc cccgctgctg ccggcaacac caccaccgga gcggccaatt
     2041 cgagcgcgct gctggcccag aagctggcca gtgcccgctt cagtgtggtg ctggggctgt
     2101 tctttctggc cggcatcggg ctcagcttca ccccctgcgt gctgccgatg attccgattc
     2161 tgtcatcgat cgtcgtcggc cgggagcaac accccacccg gatgcaggcg ctgctgctga
     2221 cgctgctcta tgtggtcagc atggcgctga cactggcagt ggccggcgcc ctcaccgggc
     2281 tctttggcgc cagactcaat ctgcaggcct atttgcagtg gccaccgctg ctggtcggct
     2341 ttgcgctgct gttcgtgctg ctgtcgctgt cgatgttcgg cctctatgaa ctgcaactgc
     2401 cggcagcgct gcgcaaccgg ttcgaccagc tcaaccggca gatgagccat ggccatctgc
     2461 tgggcgcgat ggcgatgggc gccctctcga ccctggtgat ttcgccctgc gtctcggcgc
     2521 cgctggccgg tgcgctggtc tacatcagca ccacccagga tgcctggctg ggtggtgcgg
     2581 cgctcttcgc gctcgggctg gggatgggcg ttccgctgct gctgctcgcc acgctgggga
     2641 accatctgct gcccaaggcc ggcgcgtgga tggagcgggt caagtcgttc tttggcctgc
     2701 tgctgctcgg ggtggcggtc tggctgctgg agcggctgct gcccggcccg gtcaccctgc
     2761 tgctgtgggc cgcgctgctg ctgggtggcg cactgctgct cggcgcgctg gagttcaccg
     2821 cccacccttc gggctggcac aaactgggcc agaccctcgg gctgctgctg ctggcctggg
     2881 ccgtggcgct ggtgctcggc gccgccgccg gcggttccga cccattgcgg ccgctgcatg
     2941 gcctgggcat ccgtcagggg caggcggccg ccgtggagca tcccagcttc accgacatca
     3001 ccgatctgcc ggggctcgac cggcaactga cgctggcggc cggacgcatc acgctggtcg
     3061 atgtctatgc cgactggtgc atcgcctgca aggtgctgga gcgcgaggtc tttccgacag
     3121 tcaccacgca gttggcaccg ctgcatcgac tgcgcgccga catcaccgac tatcaggccc
     3181 cgcaacgcgc cctgctcgac cgctaccgca tcgttggtcc gccgaccctg ctgttcctcg
     3241 atgcaaaggg gcaggagctg cccgattcac gcatcgtcgg cgagatcgat gccacacaac
     3301 tggccagcca cctcgaccgg ctgctgtcgc gctgagtgcg atcctgcctt gcctttcggg
     3361 cggatcggcc ctagagtgca gcggcccgga attggaaatt tgctgtcaat tgacggcaaa
     3421 cttcggcaaa ataacagtga cttgacccaa gaattccaaa accgatccgc atggcgaaac
     3481 tgctgctgct caatggcccc aacctcaaca tgctgggccg ccgcgaaccc ggccactatg
     3541 gccgccagtc actcgacgcg atcaccgatg cactggtgcg cgaggccgaa gcccgcggcc
     3601 acacgctctg ctgctttcaa tccaatgccg agcacgcgct gatcgaccgc atccacggcg
     3661 cgctcgagga tggcaccgac ttcatcctgt tcaatccggc ggccttcacc cacagcagcg
     3721 tggcgctgcg cgatgcgctg ctggcggtcg ccattccgtt catcgagctg cacctgtcga
     3781 atgtccatgc acgcgagcca tttcgtcatc actcctactt ctctgacatc gccgtcggcg
     3841 tgatcagcgg cttcggcgcc gacagctacc gtctggcact gctggccgcg atcgagcggc
     3901 tggccacgcc aaaaccatca ccctgaccca tcccgggagt acacaaccat caccatggac
     3961 atccgcaaag tcaaaaaact gatcgaactg gtcgaagagt ccggcatcga agagctcgag
     4021 atcaaggagg gcgaggagtc ggtgcgcatc ag
//
LOCUS       DAORCQ010000145         3963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_18166_length_3963_cov_5.268424, whole genome shotgun sequence.
ACCESSION   DAORCQ010000145 DAORCQ010000000
VERSION     DAORCQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18166_length_3963_cov_5.268424"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKI23_10965"
     CDS             complement(<1..793)
                     /locus_tag="PKI23_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNN37498.1"
                     /translation="MIMRNANTILIVDDVPDNLAVLHDALDESGFTVLVAVNGESALS
                     SAVEAQPDIILLDALMPSMDGFEVCRRLKADLRTQHIPVIFMTGLTESEHVVNGFVAG
                     GVDYVTKPIRISEVLARIATHLHTAHSIQQMRGVLDAFGQAAIAVLPHSGRIIWQTPL
                     ARILLHKYLGVSSDECSATPPEPLLKWLMKLSRRGSLKCGMDSTPQPLTIVQPCGRLR
                     FIPTDLSSDEQWTLLLREESDVVQIDALIARFHLTRRESEVLCWVI"
     gene            complement(798..>3963)
                     /locus_tag="PKI23_10970"
     CDS             complement(798..>3963)
                     /locus_tag="PKI23_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNN37499.1"
                     /translation="WATLAVSLIIFLTALPIGHYAARYGLDMDLLTRGAGFGYIGSTI
                     SSFVYASFTFILFALEAAIMAYALELGFGLPLQLGYLLCALVIVPLVAFGVTFISRVQ
                     MITQPIWLLLMVLPFIFILYRQPEVVGDFMKFSGRLGEGREFDPQLFGAAMALGMALI
                     PQIGEQVDFLRFMPERTRENRWRWHLGVLMAGPGWILPGMAKMLAGALLAYMALLAGR
                     SAEEAVNPNQMYLIAFGQMFSNPDLVLAVTVFFVVLSQIKINMTNAYAGSLAWSNFFA
                     RLTHSHPGRVVWLLFNIVIAIMLMELNVFQALEQVLGLYSNVAISWITAVVADLVINK
                     PLRLSPPGIEFRRAYLFDINPVGVGAMLIASTLSVLAFLGLFGLEVQPFSSFIALITA
                     LLAAPLLAWLTQGRYYIAREPVRYGAAAPTRQCVLCEKSYEVEDMAHCPAYQGAICSL
                     CCCLDARCNDLCKPDARIGRQWEAAMQRLLPKSLWRYLNSSMGHYLLLMGITLSLLAA
                     LFGLIYLHESLSLAGAAEEILPRLGLGYLKAFAALVLASGIIVWWLVLTSQSRTVAQQ
                     ESNRQTSLLQREIMLHRQTDAELQQARWVAEQANQAKSRYITGISHELRTPLNSILGY
                     AQLLDNDVTLHDSTQQAIRVIRRSGEHLLSLIEGTLDIARIEAGKLTFDIKPLKFPEF
                     IRQIVSMFELQARNKGLTFDHQISGELPAVVRADHKRLSQILINILGNAVKFTQQGGV
                     SFRVRHAREMVHFEIEDSGPGIPPDELEQVFEPFLRGSAAHGIGGTGLGLTISKLLTE
                     LMGGELRVDSRPNRGTTFGIRLFLPQVRQTQELESVVMARRTGYQGARRRVLVVDNEP
                     VDRRLLSDILQPLGFVVAEAATGHDCLRILPRFNPDLVLMDLAMPAMDGWETSYLIRK
                     MHRSDLHIGIVSANAFDKGMENMAGITAADFIFKPVNVQELLGWIGERLGLQWITEGE
                     PPGAVVETSGLTHDAVVPAQEQLDELLALVRIGHLRGITRKLDELMAADDRHQGFVRI
                     CREMTQQFQLEALKYFIKKRLAGE"
BASE COUNT          842 a         1219 c         1183 g          719 t
ORIGIN      
        1 tgatgaccca gcagagcact tcggactccc gacgggtcag gtggaaacgc gcgatcagcg
       61 cgtcgatctg caccacgtcc gactcttcgc gcagcaggag ggtccactgt tcgtcgctgc
      121 tcaggtcggt cggaatgaag cgcagccgcc cacagggttg cacgatggtc aaaggttgtg
      181 gtgtggaatc catcccacac ttgagcgacc ctcgtctcga cagcttcatc aaccatttca
      241 gcagcggttc gggaggcgtg gccgagcact catcgctcga aacacccaaa tatttgtgca
      301 acagaattct ggccagcggt gtctgccaga tgatcctgcc tgagtgcggc aacacggcga
      361 tggccgcctg accgaacgca tccagcaccc cgcgcatctg ttgaatggag tgcgcggtgt
      421 gcaaatgggt ggcaatgcgc gccagcactt cgctgatgcg tatcggcttg gtgacatagt
      481 cgacaccccc tgcaacaaag ccgttgacca catgttcgga ttcggtcagc cccgtcatga
      541 agatcaccgg aatgtgctgg gtgcgcagat cggctttcag tcgtcgacag acttcgaaac
      601 catccatgct cggcatcagc gcatcgagca gaatgatgtc cggctgggct tccactgcgc
      661 tgctgagtgc gctctcgcca ttgaccgcca ccagcacggt aaaaccggac tcatcgagcg
      721 catcgtgcag cacggcaagg ttgtccggca cgtcatcgac gatcagaatc gtgttggcat
      781 ttcgcatgat catcgactca ctcacccgcc agtctctttt tgatgaagta cttcagcgct
      841 tccaactgga actgctgtgt catctcccgg cagattctga cgaaaccttg atggcggtcg
      901 tcggccgcca tgagctcgtc gagcttgcgg gtgatgccac gcagatggcc gatcctgacc
      961 agagccagca gctcatcgag ctgctcctgg gcaggaacca ccgcgtcgtg agtcaagccg
     1021 ctcgtttcga cgaccgctcc gggcggttcg ccctcggtga tccactgcag gccgaggcgt
     1081 tcgccgatcc atcccagcaa ctcctgcaca ttgaccggct tgaaaatgaa gtcggccgca
     1141 gtgatgccag ccatgttctc catgcccttg tcgaaggcat tggccgaaac gatgccgatg
     1201 tgcagatcgg atcgatgcat cttgcggatc aggtaactgg tctcccagcc atccatggcc
     1261 ggcatggcca ggtccatcag gaccaggtca gggttgaacc ggggaaggat ccgcaggcag
     1321 tcgtgaccgg tggccgcttc ggccaccacg aaacccagag gttgcaggat gtcgctcagc
     1381 agtctgcgat cgaccggttc gttgtcgacc accagcaccc ggcgccgggc gccctgatag
     1441 cccgttcgcc tggccatgac aaccgactcc agctcctggg tctgtctgac ttgcggcagg
     1501 aacagccgga tgccaaaggt ggtgccccga ttcggtctgc tgtcgacccg cagttcacca
     1561 cccatcaatt cggtcagcag cttgctgatc gtcagtccca gtccggtgcc gccaatgcca
     1621 tgggcggcgc tgccgcgcag aaagggttcg aagacctgct ccagttcatc ggggggaatg
     1681 cccggaccac tgtcctcgat ctcgaaatgg accatctccc gcgcatggcg aacgcggaag
     1741 ctcacgccgc cctgctgggt gaacttgacg gcattgccga ggatattgat cagaatctgg
     1801 ctgagccgct tgtggtctgc tctgaccacg gccggcaact caccggaaat ctgatggtcg
     1861 aaggtcagcc ctttgttgcg ggcctgcaat tcgaacatgc tgacgatctg gcggatgaat
     1921 tccggaaact tcaaaggttt gatgtcgaag gtgagttttc cggcctcgat gcgtgcgatg
     1981 tccagggtgc cctcgatcag cgacagcaga tgttcaccgc tgcgccggat gacccggatc
     2041 gcctgctgtg tgctgtcgtg gagcgtcaca tcgttgtcga gcagctgggc ataacccaga
     2101 atgctgttga gtggtgtgcg cagctcatgg ctgatgccgg tgatgtagcg gctcttggcc
     2161 tgattggcct gttcggccac ccagcgtgcc tgctgcaact cggcgtcggt ctgacggtgc
     2221 agcatgattt cgcgctgcaa cagactggtc tgccggttcg actcctgctg ggcgacggtg
     2281 cggctttgtg aggtcagcac cagccaccag acgatgatgc cgctggccag caccagcgcg
     2341 gcaaaggcct tgagataacc cagcccgagc cggggcagaa tctcctcggc cgcgcccgcc
     2401 agactcagtg actcatgcag atagatcaat ccgaacaggg ccgccaacag tgacagggtg
     2461 atgcccatca gcagcagata gtgccccatg ctgctgttga gatagcgcca cagcgacttg
     2521 ggcagcagcc gctgcatcgc tgcttcccac tgtcgaccaa tgcgcgcatc gggcttgcag
     2581 aggtcattgc agcgcgcatc caggcaacag cagagcgagc agatcgcccc ctgataggca
     2641 gggcaatgcg ccatgtcctc gacttcgtaa ctcttctcgc acagcacgca ctgccgggtc
     2701 ggcgccgctg caccgtagcg gaccggttca cgggcgatgt aatagcgccc ttgcgtcaac
     2761 caggcgagca gcggggcggc aagcagcgcc gtgatcagtg cgatgaatga ggagaatggc
     2821 tgaacctcca ggccgaacag gcccagaaat gccagcaccg acagcgtgga ggcaatcagc
     2881 atcgcgccga caccgaccgg attgatgtcg aacaggtagg cacgacggaa ttcgatgccc
     2941 ggcggactca accgcagtgg cttgttgatg accagatcgg ccaccacggc ggtgatccac
     3001 gaaatcgcca cattcgaata gagtccaagc acctgttcga gcgcctgaaa cacattcagc
     3061 tccatcagca tgatggcgat cacgatattg aacagcagcc agaccacccg tcccggatga
     3121 ctgtgggtca ggcgggcaaa gaagttcgac caggccagcg aaccggcata ggcattggtc
     3181 atgttgatct tgatctgcga cagcacgacg aaaaaaaccg tcaccgccag caccagatcg
     3241 ggattcgaaa acatctggcc aaaggcgatc aggtacatct ggttgggatt gaccgcctcc
     3301 tcggccgatc gacccgccag cagcgccatg taggccagca gcgcaccggc cagcatcttg
     3361 gccatgcccg ggagtatcca cccgggtccg gccatcagca caccaaggtg ccagcgccag
     3421 cgattctcac gggttcgctc tggcatgaac cgcagaaaat cgacctgctc accgatctgc
     3481 ggaatcagtg ccatgccgag cgccatcgcc gcaccaaaca gttgcgggtc aaattcacgc
     3541 ccctccccca gcctgccgga gaatttcatg aaatcgccaa cgacctcggg ttggcgatag
     3601 agaatgaaga tgaacggcag caccatcagc aacagccaga tcggctgggt gatcatctgc
     3661 acacggctga tgaaggtcac gccaaaggcg accagtggca cgatcaccag tgcgcagagc
     3721 aggtagccga gctgaagcgg cagtccaaaa ccgagttcga gcgcgtaggc catgatggcg
     3781 gcttcgagcg cgaacagaat gaaggtgaag gaggcataga cgaacgagga gatcgtcgag
     3841 ccgatgtagc caaaaccggc gccacgggtc agcagatcca tgtccagccc gtaacgggcc
     3901 gcgtagtgac cgatcggcag agcggtcagg aagatgatca ggctgactgc cagcgtggcc
     3961 cac
//
LOCUS       DAORCQ010000146         3895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_18691_length_3895_cov_4.552083, whole genome shotgun sequence.
ACCESSION   DAORCQ010000146 DAORCQ010000000
VERSION     DAORCQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18691_length_3895_cov_4.552083"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..542
                     /locus_tag="PKI23_10975"
     CDS             3..542
                     /locus_tag="PKI23_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37500.1"
                     /translation="MIGLTLALLMAATRSQHFASLETLPSASWAVFFLAGLYLTPRWA
                     VVGLLAEAALLDYVAITWGGVSSFCVTPAYLFLLPAYGTLWLAGRWYGQRHLHAIKPL
                     PLLGSLTLAGVVCELWSGGSFYFLGGRFGETSLAVFGQRLLTYLPSELFDLFFYVAIA
                     VLLQWLVARAAGRLRPVSA"
     gene            complement(589..1287)
                     /locus_tag="PKI23_10980"
     CDS             complement(589..1287)
                     /locus_tag="PKI23_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37501.1"
                     /translation="MPPHVTIHQRFVGPPGMGNGGYVAGAVAAFIEGPARIKLARPTP
                     LDRPLPIVRTPSGGALLQDQGETLVEGEPTQLTLSVPVPPDFAAARRLMADSVALQRP
                     VHPICFVCSAQRDENDGLRIHPGRVEDTMMVAAAWTPDASLADARGLIDPIFLWSALD
                     CPGAYAFMIDRKPGFLGTLSGSIEGEIRPGEECVVIGWQIGSSGRKLLAGSAIFNAAN
                     QLCGRAEAIWIEAG"
     gene            complement(1312..1977)
                     /locus_tag="PKI23_10985"
     CDS             complement(1312..1977)
                     /locus_tag="PKI23_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37502.1"
                     /translation="MSPAIEFPDRETRARLRSEVLDAIALHRARHPLQQQIEAAEPAR
                     QRAYLALHEACREERLAAQLDALDPATRAWLQQIAAIHASTDGWNCPPFTSIESPFII
                     ETASTRYHAASAIDALAMPALLGCDGTLHGHCRQCGVGLSHRINRQGGLDELAIRQLG
                     VITDPADSAAVPDTRRPPSWLICADCPPPDTVIRLPLPPASTIGNAFYGFIGRLRCAL
                     NSP"
     gene            complement(1990..2649)
                     /locus_tag="PKI23_10990"
     CDS             complement(1990..2649)
                     /locus_tag="PKI23_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37503.1"
                     /translation="MKKLTLAGTTLTALLLAPLGHAATTLAVMDFSFSHELPSPATAA
                     NALSIRFSYSVQPGGLTDVDSLIFDALSFDGSAAGAVHTLTDNADDANYSNFLGYLLS
                     PAIDDIRVDVIGNQGGGIGIVGPESSLLRTAQGAPFSGLPANSLISALQLSISQIDID
                     PSASSGAFNWAVTGQLRVLGEVAPVPLPGAAPLFGAAALLTLAVSARRHRARPVPFEH
                     S"
     gene            complement(2703..>3895)
                     /locus_tag="PKI23_10995"
     CDS             complement(2703..>3895)
                     /locus_tag="PKI23_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN37504.1"
                     /translation="SGTTGRPKGVVRDNGGHAVALKYSMRAIYDCGIGDTFWAASDVG
                     WVVGHSYIVYGPLFAGCTTLLYEGKPVRTPDAGAFWRVIAEHQVRAFFTAPTAMRAIK
                     KEDPEGHLLARHDIGCLKQLFLAGERLDPPTYHWLRRLLPVPVIDHWWQTETGWAIAG
                     NPTGIEMQPSKPGSATLPMPGFDVRILDAAGAPLPAGQQGSVVIRQPLPPGCLPTLWG
                     DHPRFVAAYLAPYPGYYLSGDGGYFDEEGHLFIMGRTDDVINVAGHRLSTGEMEEVIA
                     AHPAVAECAVVAIKDELKGQLPLALVVMKDGAEIEPEALEQALVATVRQQIGPIACFR
                     RALVVKRLPKTRSGKILRNVLRQIADHEPYPMPATIDDPKVLDEIGELLGNGQQSSDS
                     TVHP"
BASE COUNT          621 a         1243 c         1359 g          672 t
ORIGIN      
        1 ggctgatcgg gctgacactg gcactgctga tggcggccac ccgcagccag cactttgcca
       61 gcctcgagac gctgccgagc gcctcctggg cggtcttctt tctggccggt ctctacctga
      121 cgccgcgctg ggcagtggtc gggctgctgg ccgaggcagc gctgctcgac tatgtggcca
      181 tcacctgggg tggggtcagc agcttttgtg tcacaccggc ctacctcttt ctgctgccgg
      241 cctatggcac gctctggctg gccgggcgct ggtatggaca gcgtcatctg cacgccataa
      301 agccactgcc cttgctgggc agcctgacgt tggcgggggt ggtctgtgag ctctggtcgg
      361 gtggcagctt ctactttctg gggggacggt ttggtgaaac ctcgctggca gtgttcgggc
      421 agcggctgct gacctatctg ccgtcagagc tgttcgacct gttcttctat gtcgccattg
      481 ccgtgctgct gcagtggctc gttgcccgcg cggctggtcg cctgcggccg gtatcggcct
      541 gacgccgttg cggacgcggt gccctgccgc gtccggaatc accttgcctc agcccgcctc
      601 gatccagatc gcctcggcgc ggccgcagag ctggttggcg gcgttgaaga tggcgctgcc
      661 ggccagcagc ttgcgcccgg agctgccgat ctgccagccg atcaccacac actcctcgcc
      721 gggtctgatc tccccttcga tcgatccgct gagggtgccg agaaaacccg gcttgcggtc
      781 gatcatgaag gcgtaggcgc ccgggcagtc gagcgccgac cagagaaaga tcggatcgat
      841 caggccacgg gcatcggcca gtgatgcatc gggcgtccag gcggcggcca ccatcatcgt
      901 gtcctccacc cgccccggat gaatccgcag accatcgttt tcatcgcgct gcgcactgca
      961 gacgaagcag atcggatgca ccggacgttg cagcgccacc gaatcggcca tcagccgccg
     1021 ggcggcggcg aaatcggggg ggacgggaac gctcagcgtc aactgcgtcg gttcaccctc
     1081 gaccagcgtc tcgccctgat cctgcaacag tgcgccgcca ctgggggtgc gcacgatcgg
     1141 cagcgggcgg tcgagggggg tcggtctggc cagtttgatc cgtgccggac cctcgatgaa
     1201 tgccgccacc gcaccggcga catagccgcc attgcccatg ccgggtgggc cgacgaaacg
     1261 ttgatgaatg gtcacatgtg gcggcatcga aggaacctcg cggttgggtt gtcaggggct
     1321 gttcagggcg caccgcagcc ggccgatgaa gccgtagaag gcattgccga tggtcgaggc
     1381 gggcggcagc ggcaggcgga tcactgtatc gggtggcggg cagtcggcac agatcagcca
     1441 gctcggtggc cgtcgggtgt ccgggaccgc cgcgctgtcg gcagggtcgg tgatcacacc
     1501 gagctggcga atggccagct catcgagccc gccctggcgg ttgatacggt ggctgagccc
     1561 cacgccacac tgccggcagt ggccatgcag cgtgccgtcg cacccaagaa gcgccggcat
     1621 cgccagcgcg tcgatggcgc ttgccgcgtg gtagcgggta ctggcggttt cgatgatgaa
     1681 cggactttcg atcgaggtga acggcgggca gttccagccg tcagtgctgg catggatggc
     1741 cgcgatctgc tgcaaccagg cgcgagtcgc cggatcgagg gcgtcgagct gcgccgccag
     1801 ccgctcctcg cggcaggcct cgtgcagcgc cagataggcg cgctgccgcg ccggctcggc
     1861 tgcttcgatc tgctgctgca gtggatggcg cgcgcggtgc agcgcaatgg catcgagcac
     1921 ctcgctgcgc agccgggcgc gcgtttcgcg gtcgggaaac tcgatggctg gactcatcgg
     1981 ttcggccgat caggaatgct cgaacggcac tggtctggct ctgtgacggc gggccgacac
     2041 cgccagcgtc agcagggctg cggcaccaaa caggggggca gcccccggca gcggcaccgg
     2101 tgccacttca cccaggaccc gcaactggcc ggtgaccgcc cagttgaacg ccccggagct
     2161 ggcggacgga tcgatgtcga tctggctgat cgagagttgc agtgccgaaa tcaggctgtt
     2221 ggcgggcagc ccgctgaagg gagcgccctg ggcggtgcgc agcagcgagg attccggacc
     2281 cacgatgccg atgcctccgc cctggttgcc gatgacatcg acccggatgt catcgattgc
     2341 cgggctcagc agatagccga gaaagttcga atagttggca tcgtcggcat tgtcggtcag
     2401 cgtgtggaca gcgccggcgg cactgccgtc gaagctcagg gcgtcgaaga tcaggctgtc
     2461 gacatcggtc aggccgccgg gttgcaccga ataggagaag cggatcgaca gcgcattggc
     2521 tgccgtggca ggggagggca gctcatggct aaaagagaaa tccatcaccg ccaaggtggt
     2581 ggcggcatgg ccgagcggtg cgagcagcag ggccgtcagg gtagtgccgg ccagggtcaa
     2641 ttttttcatt gcattcaact cctgtggtga aatcagtcag cgagaatggg gtctgcaaac
     2701 cttcatggat ggacggtgct gtcgctgctc tgctgcccat tgcccagcag ttcaccgatc
     2761 tcatccagca ccttcgggtc gtcgatggtg gctggcatcg ggtagggctc gtggtcggcg
     2821 atctgccgca gcacgttgcg caatatcttg ccggagcggg tcttgggcag ccgcttcacc
     2881 accagcgcgc ggcgaaagca ggcgatcgga ccgatctgct ggcgcaccgt tgcgaccagt
     2941 gcctgctcca gtgcctcggg ctcgatctcg gcgccatcct tcatcaccac cagcgccagt
     3001 ggcaactggc ccttcagctc atccttgatc gccaccacgg cgcactcggc cacggccgga
     3061 tgggcggcga tcacctcctc catctcgccg gtcgagagcc ggtggccggc gacattgatc
     3121 acatcgtcgg tgcgacccat gatgaacagg tggccctctt cgtcgaaata accaccatcg
     3181 ccgctgagat agtaaccggg gtagggggcc agataggcgg cgacgaagcg tggatggtcg
     3241 ccccacagcg taggcagaca gccgggtggc agcggctgcc ggatgacgac actgccctgc
     3301 tggcccgccg gcagtggcgc cccggcggca tcgaggatgc gcacatcgaa gccgggcatc
     3361 ggcagggtgg ccgagcctgg tttgctcggc tgcatctcga tgccggtcgg attgccggca
     3421 atcgcccagc cggtctcggt ctgccaccag tggtcgatca ccggcaccgg cagcagcctg
     3481 cgcagccagt ggtaggtggg tggatcgagc cgctcgccgg ccagaaacag ctgcttgagg
     3541 cagccgatgt cgtgccgggc cagcagatgg ccttcgggat cctccttctt gatcgcgcgc
     3601 attgcggtgg gtgcggtgaa gaaggcccgc acctgatgct cggcgatcac ccgccagaag
     3661 gcgccggcgt cgggcgtgcg caccggcttg ccttcgtaga gcagggtggt gcaaccggca
     3721 aacagcggcc cgtagacgat gtaggagtgg cccaccaccc agccgacatc cgaggcggcc
     3781 cagaaggtgt cgccgatgcc gcagtcgtag atcgcccgca tgctgtattt gagggccacg
     3841 gcatggccgc cgttgtcgcg caccacccct ttggggcgac cggtggtgcc ggagg
//
LOCUS       DAORCQ010000147         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_18869_length_3872_cov_5.869007, whole genome shotgun sequence.
ACCESSION   DAORCQ010000147 DAORCQ010000000
VERSION     DAORCQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18869_length_3872_cov_5.869007"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKI23_11000"
     CDS             <1..601
                     /locus_tag="PKI23_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37505.1"
                     /translation="VPLDGGVFSAFGMLVAPQARQLSRSCIQPFDDASGTAIDALLAR
                     LDASGVADLRAQRVDPATLQRLASVDLRYRGQSFTLNLPWQGVDATGSAFHALHEQRY
                     GHRLEMPIELVNVRLLLRAPARPWPLPPWQPTATPSAQSAPLAGFAQPVPIRQRESLR
                     VHEAIEGPLLLTERTSTTLLLAGWRGELDLQGHLLLNRE"
     gene            complement(626..925)
                     /locus_tag="PKI23_11005"
     CDS             complement(626..925)
                     /locus_tag="PKI23_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37506.1"
                     /translation="MHNESNAFEQAAQRSIDLANELAERQIDADLWEIADGTLSGAIH
                     YWLFSRQPCPDRDCDDCAPVATAELRLQTLLKLVRELAEESLYYHSPSDHNAAHG"
     gene            1248..1973
                     /locus_tag="PKI23_11010"
     CDS             1248..1973
                     /locus_tag="PKI23_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNN37507.1"
                     /translation="MQLLEQIEQTGSITQAAKSLSMSYRAAWNAVDALRNLAGEALLV
                     TQSGGSDGGGTRLTPAGKRLLHTHRAVQEQQRRFMSGLHAQLDHPDILPLLRRFAVKT
                     SARNQFFGTVTGIRLGAVNAEVTVALNDRDRLVATVTMESLGELQLGPGSEVWTLVTA
                     PSVMVMPDDPAIKLSARNRLCGTVSRITRGAVNSDVVIDLPGGSAVSAIITNDSLDSL
                     ALAVGTPACAVFKAGSVILGVNA"
     gene            2011..3342
                     /locus_tag="PKI23_11015"
     CDS             2011..3342
                     /locus_tag="PKI23_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNN37508.1"
                     /translation="MPRPTLIAATLAAIFAFSQAQAADASANIESMQQRLDQLERLVG
                     QLQSQLDAERQARQKVESAAVIRPVTDGKSLKFQSPSGDFSFQAGGRIDVDTAHYDQD
                     KQDLGNGTDFRRARMYMRGTLARDFDYLFEYEFVDSAARNKGITDAWLRYKGFAPALL
                     TVGNFKMPFGMEQLTSDNTTIFLERGVNDILTPGRGIGAELRSAGSNWSLAGGLFGEK
                     PDGDVDKEGDEGRDVAARTTYAPILKPDTVLHLGAGVRRHDPNDSSNALRFRSRPESN
                     LTGVYLVDTGILANTDDFVSTNAELASRWGPVTFQSEYMHTALERDDTAGDADFSGWY
                     AQAAWLMTGERRPYKVSEGVFDRVTPNGSIGLGGFGAWELAARLSEVDLNDSGIVGGQ
                     ERNLTLAVNGYLTSNIRVMFNYIRVLELDRPGNAANHDEPSILGTRLHIDF"
     gene            3371..>3872
                     /gene="modA"
                     /locus_tag="PKI23_11020"
     CDS             3371..>3872
                     /gene="modA"
                     /locus_tag="PKI23_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNN37509.1"
                     /translation="MPCSIPRRVALTAVLLLALPAACAEEVVAAVAANFAAAMARIEP
                     AFEQASGHQLTVVLGSSGKFLQQIQQGAPFDVFLSADAERPELLARSNLGVPASRFTY
                     AIGRLALWSPQADAFSDGETYLKAGAFRHLAIANPAVAPYGVAAQRTLEQLGLWAGLQ
                     EKIVRAE"
BASE COUNT          676 a         1354 c         1231 g          611 t
ORIGIN      
        1 ggtgccgctc gatggcggcg tcttctcggc gttcggcatg ttggtggcac cgcaggcccg
       61 gcaactgtca cgcagctgca tccagccatt cgacgacgcc agcggcaccg ccatcgatgc
      121 gctgctggca cggctcgatg ccagcggtgt ggccgatctg cgcgcacagc gggtcgatcc
      181 ggccacgctg caacggctgg cctcggtcga tctgcgctac cgcgggcaat ccttcacgct
      241 caacctgccg tggcaaggcg tcgatgcgac cggcagcgcc tttcatgcgc tgcatgagca
      301 gcgctatggc caccggctgg agatgccgat cgaactggtg aatgtccggc tgctgctgcg
      361 cgcgccggcc cgaccctggc cgctgccacc ctggcagccg accgccacgc catcagcgca
      421 gtcggcgccg ctcgccggct ttgcgcagcc ggtgcccatc cgccagcggg agtcgttgcg
      481 ggtgcatgaa gcgatcgagg ggccactgct gctcaccgag cgcacctcga ccaccctgct
      541 gctggccggc tggcggggag agctcgatct tcaggggcat ctgctgctga accgggagtg
      601 acctcccggc agcaggcatc agaggtcagc cgtgggcggc gttgtggtcg ctcgggctgt
      661 ggtagtagag gctctcttcg gccagctcgc ggaccagctt cagcagcgtc tgcagccgca
      721 gttcggcggt cgccaccggt gcgcagtcat cacagtcacg gtcggggcag ggttggcgcg
      781 aaaagagcca gtagtggatc gcccccgaca gcgtgccatc ggcaatctcc cacagatctg
      841 catcgatctg ccgctccgcc agctcattgg ccagatcgat cgaccgctgg gcggcctgct
      901 caaaggcatt ggattcgttg tgcatgagga actcccgctg gcgcagtctg gttgcagcgg
      961 cgcaagcata gcaagaatcg accagcaacg ataacgcaac cgccttggcg aaggggcttc
     1021 caccggcacg atcgcagccg acgggtgccc ctcctcggtt cctgacagat ccttccgaac
     1081 cttcagcgcc caccaaggat tgcagcggat cttgtgcagg gtgaaacagc ctgctaccgt
     1141 tatgcacata tctgcataac ggtagcaggg cgatgcgcaa acagcgggcg aacaacaaaa
     1201 cggtcgagcc cgtttcacct cagtggagcc tccctgacga ccgtcgaatg cagttgctgg
     1261 agcagatcga gcagaccggc tcgatcaccc aggccgccaa aagcctgagc atgagttacc
     1321 gcgctgcctg gaacgcggtc gatgcgctgc gcaatctggc cggcgaggcg ctgttggtga
     1381 cgcaaagcgg cggcagcgat ggcggcggca cgcggctgac gccggctggc aaacggctgc
     1441 tgcacaccca ccgcgcggtg caggaacagc agcgccgctt catgagcggg ctgcatgcac
     1501 agctcgacca ccccgacatc ctgccactgc tgcggaggtt cgccgtgaaa accagtgccc
     1561 gcaatcagtt cttcggcact gtgaccggca tccgcctcgg tgcggtgaat gccgaggtga
     1621 ccgtcgccct gaacgaccgc gatcgtctgg tcgccaccgt cacgatggag agtctgggtg
     1681 aacttcagct cggccctggc agcgaggtgt ggacgctggt cacggcgccc tcggtgatgg
     1741 tcatgcccga cgacccagcc atcaaactat cagcccgcaa ccgtctgtgc ggcaccgtct
     1801 cgcgcatcac ccgcggcgcg gtcaacagcg acgtcgtcat cgatctgccg ggcggcagcg
     1861 cggtcagcgc catcatcacc aacgacagcc tcgacagcct ggcgctcgcc gttggcacgc
     1921 cggcctgcgc ggtgttcaag gccggcagcg tgatcctggg cgtcaacgcc tgagcccccc
     1981 gaccaatcca catatcccgg ggaagacacc atgccacgcc cgaccctcat cgccgcaacg
     2041 ctggcggcga tcttcgcatt cagccaggca caggctgccg atgcatcggc caacatcgag
     2101 tccatgcagc agcggctcga ccagctcgaa cggctggtcg gccaactgca atcccagctc
     2161 gatgccgagc gccaggcacg gcaaaaggtc gaatcggccg ccgtgatccg cccggtcacc
     2221 gatggcaaga gcctgaaatt ccagtcgccg agcggcgact tcagcttcca ggccggcggc
     2281 cggatcgatg tcgacaccgc gcattacgac caggacaagc aggatctggg caacggcacc
     2341 gatttccgcc gcgcgcgcat gtacatgcgc ggcacgctgg cgcgcgattt cgactacctg
     2401 ttcgagtatg aattcgtcga ctccgccgcg cgcaacaagg gcatcaccga tgcctggctg
     2461 cgctacaagg gcttcgcccc ggcattgctc acggtgggca acttcaagat gcccttcggc
     2521 atggagcagc tcacgagcga caacacgacg atcttcctgg agcgcggcgt caacgacatc
     2581 ctgacacccg gccgcggcat cggcgccgaa ttgcgcagcg ccggcagcaa ctggagtctg
     2641 gcgggtggtc tgttcggcga aaagcccgac ggcgacgtcg acaaggaagg cgacgaaggc
     2701 agggatgtcg cggcccgcac cacctacgcg cccatcctga agcccgatac ggtgctgcac
     2761 ctcggcgccg gcgttcgccg acacgatccg aacgactcca gcaacgcgct gcgctttcgc
     2821 agtcgaccgg agtcgaacct gaccggcgtc tatctggtcg acaccggcat cctcgccaac
     2881 accgatgact tcgtctccac caatgcggaa ctggcgagcc ggtggggccc ggtgaccttc
     2941 cagtccgaat acatgcacac ggcactcgaa cgcgacgaca ctgcggggga cgcggacttc
     3001 tcgggctggt acgcgcaggc ggcatggttg atgaccggcg aacgtcgccc ctacaaagta
     3061 tccgaaggcg tgttcgatcg cgtcacgccg aacggcagca tcggtcttgg tggcttcggc
     3121 gcctgggagc tcgccgcccg cctcagcgag gtggatctga acgacagcgg catcgttggt
     3181 ggccaggaac gcaatttgac gctggcagtc aacggctacc tcacctccaa catccgcgtc
     3241 atgttcaatt acatccgcgt gctggagctc gaccggcccg gcaacgcggc caaccacgac
     3301 gagccgtcga tcctcggcac gcgtctgcac atcgatttct gacccacacg actcatcgag
     3361 gagctgcacc atgccctgtt cgatcccgcg ccgcgtcgcg ctcaccgccg tgctgctgct
     3421 cgccctgccc gccgcctgcg ccgaggaggt cgtcgccgcg gtggccgcca acttcgccgc
     3481 cgcgatggcg cgcatcgagc ccgccttcga gcaggccagc ggccaccagc tcaccgtcgt
     3541 gctcggctcg tccggcaagt tcctgcagca gatccagcag ggcgcgccgt ttgacgtgtt
     3601 cctgtcggcg gacgccgagc ggccggagct tctggccagg tcgaacctcg gcgtgccggc
     3661 ctcgcgcttc acctacgcca ttggccgact cgcgctgtgg agcccgcagg ccgacgcctt
     3721 cagcgacggc gagacgtacc tgaaggccgg cgcgttccgg catctggcca tcgccaaccc
     3781 ggccgtcgcg ccctacggcg tggccgcgca gcgcacgctc gagcagcttg gcctgtgggc
     3841 cggcctgcag gagaagatcg tccgcgccga gg
//
LOCUS       DAORCQ010000148         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_19078_length_3843_cov_7.862724, whole genome shotgun sequence.
ACCESSION   DAORCQ010000148 DAORCQ010000000
VERSION     DAORCQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19078_length_3843_cov_7.862724"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..435
                     /locus_tag="PKI23_11025"
     CDS             160..435
                     /locus_tag="PKI23_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37510.1"
                     /translation="MSNPNLHPVWAKVLGITPQTTSPSTPAETQLSPLAAAIAAMTGQ
                     TNSHQGWQATSHPRRPGAPVGIKKQVGPGLWVSTVARPGDTHYPVHP"
     gene            457..1302
                     /locus_tag="PKI23_11030"
     CDS             457..1302
                     /locus_tag="PKI23_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37511.1"
                     /translation="MKLNPVSKKSNPYLDKIKSENDALNLELTPLKAELAEAEAEHAA
                     AREKQNRLRDAAGSLSMNTPPAAKAHWPILCEANQRMERLKSKVSNLESQLRPLQQVL
                     ATPERFAVARKQLDDLIAQRKALTAEGQTVDGQLTKIAKRLANLEARIAVETKSASRA
                     LLDTEAEFVVPETLTKLEVELRITRASQVELERQRDAIQGQLAGLPDAVRKARDHFIH
                     CRAAMAEIELHEQLMPVMNALARASAARRQINYHHDESRFPVETPRDLIEAASDALAA
                     EMPAP"
     gene            1453..1875
                     /locus_tag="PKI23_11035"
     CDS             1453..1875
                     /locus_tag="PKI23_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37512.1"
                     /translation="MNINLSISIPGQPLADYDPEVAQRVQDWLASGQLLVWIPILSQQ
                     NFPETPPEILQFADRHQESMRWPIHAPEDLLQLPEGITGWLLPANAEDTDVACGVWNG
                     EVYILGVKDKRTGRAQTLLGERFDVATGQVDDVDQSAV"
     gene            1937..2185
                     /locus_tag="PKI23_11040"
     CDS             1937..2185
                     /locus_tag="PKI23_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37513.1"
                     /translation="MSTHQTLRVQVIDTNQRPRGVMTIEADFDHLGPYRVQHDGQAYW
                     FTGKSGTHRVSGVATREMATADDARLWITLGGTAIWED"
     gene            2188..3066
                     /locus_tag="PKI23_11045"
     CDS             2188..3066
                     /locus_tag="PKI23_11045"
                     /inference="COORDINATES: protein motif:HMM:NF022598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HNN37514.1"
                     /translation="MAAEQQHTIPAQLAALPSLPMVELWALWDQHFPRRPSHRNRNYV
                     EWRLAYRIQELAYGALPKGGKSFTKQTIYKMLHNRMYLGDIVHKGQAFPGQHQAIITQ
                     AQWDAVHALIATDGIERRRETNDRRREPVLLRGLLFTPDGERLVPSYTVKKGKTYRYY
                     SPIKHRRFGAWASQHGPLPAAPIEELVTQQIVAALSAPHIVQSVWDRIRTARPDLSEP
                     EVVLPMRNLAGLWQQLFPAEQCRLAQLLIERVVIADGGLEIIWRDHGWQELAGELLPG
                     SIGAELQEWEQTEEMA"
     gene            3063..3515
                     /locus_tag="PKI23_11050"
     CDS             3063..3515
                     /locus_tag="PKI23_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase resolvase"
                     /protein_id="HNN37515.1"
                     /translation="MKPKVQALGGPVARELRDGGEVKLSTFIPLKIRKRGAIKVVVRP
                     NGAVESPGKVASQIDQPLLVALTRAFYWQQLLDDGVVGSGSEIAQREGLHHSTVNELL
                     RLTLLEPAIIQSILAGQQPKCMSLLWFQRNPLPTDWVAQREVVAGFDA"
     gene            complement(3564..3725)
                     /locus_tag="PKI23_11055"
     CDS             complement(3564..3725)
                     /locus_tag="PKI23_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37516.1"
                     /translation="MTQSTELTSAGDDEQIRLEPDQHEMAAPARKCIERAFVDSLAQF
                     DELYRQLAR"
BASE COUNT          772 a         1265 c         1182 g          624 t
ORIGIN      
        1 tgcgccgcat cgccccggcc taccgcgcca agggcatcga catcacccat ctcggccaca
       61 gccgggaggg ggcgcgctgg cgcatcgcac agacccgttc ccaaaccaat cctgccacgc
      121 cacccggcat cgagggtggc actcgatatg gagctcaccg tgagtaatcc caacttgcat
      181 cccgtctggg ccaaggttct cggcatcacg ccgcagacca ccagcccatc cacccctgcc
      241 gaaacgcagc tgtccccgct ggctgccgcg attgcggcga tgaccgggca aaccaactcc
      301 caccagggtt ggcaggccac cagccatccc cgccgccccg gcgcgccggt cggcatcaag
      361 aagcaggtgg gccccggcct gtgggtcagc accgtcgcgc ggcccggcga tacccactac
      421 cccgtccatc cctgatcaaa cccggagacc acctccatga aactgaaccc ggtcagcaag
      481 aagtccaacc cctacctcga caagatcaag tctgagaacg acgcgctcaa tctggaactc
      541 acgcccctca aggccgaatt ggccgaggcg gaagcggagc acgctgcggc acgcgagaag
      601 caaaaccggc tgcgtgatgc cgctggctct ctgtcgatga acacgccacc ggcagccaaa
      661 gcccactggc ccatcctgtg cgaggccaat cagcggatgg aaaggctcaa gtcgaaggtg
      721 tccaacctgg agagccaact gcgtccgctg cagcaggtcc tggccacacc agagcgcttc
      781 gcagtggccc gcaaacaact tgatgatctg atcgcgcagc gcaaggcgct caccgctgag
      841 gggcagacgg tcgatggcca actgaccaag atcgccaagc ggctggcgaa cctcgaagcg
      901 cgcattgctg tcgagacgaa gtcggcatct cgggcgctgc tggacaccga agcggaattc
      961 gtggtgccag aaaccttgac caaactggag gtggagttgc gcatcacccg ggcatcgcag
     1021 gttgaacttg agcgccagcg tgatgcgatt caaggtcaac tggctggcct gcccgatgcc
     1081 gtgcgcaagg cgagggacca cttcatccac tgccgtgcgg caatggccga aatcgagctg
     1141 cacgaacagt tgatgccggt catgaacgca ctggctcgcg cctcggcggc ccggcgccag
     1201 atcaactacc accacgacga aagtcgcttc ccggttgaga ctccacgcga tctgatcgaa
     1261 gctgccagcg atgcactggc ggcagaaatg ccagcgccct gaccgtcacg cgacctgccc
     1321 acaggcgaat tgggtccttc ctcgccatca agcaatacgg ggggcgcgag cgcgcgacct
     1381 cgccagcaac agacctcgat accgggttac cagctcaggt taccggttac caccccattt
     1441 cacggacacg atatgaacat caacctttcc atctccatcc ctggccagcc attggccgat
     1501 tacgaccctg aagttgcgca gcgcgtgcag gactggcttg ccagcgggca gctgctggtc
     1561 tggattccga tactgagcca gcaaaacttc cccgaaacac cgccagagat tctgcagttt
     1621 gccgaccgac accaggagtc gatgcgctgg ccgatccacg cgccggagga tctgctgcaa
     1681 ctgcccgagg gcatcaccgg ctggttgctt cccgccaacg ctgaagacac cgacgtggct
     1741 tgcggggtct ggaatggcga ggtctacatc ctgggtgtca aggacaaacg caccgggcgt
     1801 gcgcagaccc tgctgggcga gcgtttcgat gtagcgacag gtcaggtcga tgacgttgat
     1861 cagagcgcgg tttgacttgg cttctggtca tcacagcgcg ttcatggagg tccgcaaacc
     1921 cctctcggag aaagcaatga gcacccacca aaccctgcgc gtacaggtta tcgacaccaa
     1981 ccagcgcccg cgcggcgtga tgaccatcga ggccgatttc gatcacctcg ggccctaccg
     2041 cgtacagcac gatggccaag cctactggtt caccggcaaa tccggcaccc accgcgtcag
     2101 tggcgtggcc acccgcgaaa tggcgaccgc tgacgatgcc cgcctgtgga tcacgctcgg
     2161 tggcaccgcg atctgggagg actgaggatg gccgccgaac agcaacacac catcccggcc
     2221 cagttggcgg cgctgccctc actgccgatg gtcgagctct gggcgctgtg ggatcagcac
     2281 ttcccccggc ggcccagcca tcgcaatcgc aattacgtgg agtggcgtct ggcgtaccgg
     2341 attcaagagc tcgcctatgg cgcgctgccc aagggtggca agtctttcac gaagcagacc
     2401 atctacaaga tgctgcacaa ccggatgtac ctgggggaca tcgtgcacaa ggggcaggct
     2461 tttccgggtc agcatcaggc catcatcacc caggcgcagt gggatgctgt gcacgccctg
     2521 atcgccaccg atggcatcga gcggcggcgc gagaccaacg accgccggcg tgaaccggtg
     2581 ctgctgcgag gcctgctgtt cacgcccgat ggtgaacggc tggtgcccag ctacaccgtc
     2641 aagaagggca agacctaccg ctactacagc ccgatcaagc accggcgctt tggcgcgtgg
     2701 gccagccagc atgggccact accggcggcg cccattgagg aattggtgac ccagcagatc
     2761 gtcgccgcgc tgtcagcgcc gcacatcgtg cagtcggtgt gggatcggat tcggacagcc
     2821 cgcccagacc tgtcggagcc tgaggtggtc ttgccgatgc gaaatctggc tggcctatgg
     2881 caacagttgt tccccgccga acagtgccgc ctggcgcagt tgctgatcga gcgcgtggtg
     2941 attgccgacg gcgggctgga gatcatctgg cgcgaccacg gctggcagga actggccggg
     3001 gaactgctgc ccggcagcat cggtgcggaa ttgcaggaat gggagcagac ggaggaaatg
     3061 gcatgaagcc gaaagtgcag gctttgggtg gccccgttgc gcgcgaactt cgggacggcg
     3121 gcgaggtgaa actgtccacc ttcatcccgc tgaagatccg caaacgcggc gccatcaagg
     3181 tggtggtgcg gccgaatggg gcggtcgagt cgccgggcaa ggtcgccagc cagattgacc
     3241 agcccttgct ggtggccctg acgcgggcct tctactggca gcaactgctc gacgacggcg
     3301 tggttggcag cggcagcgaa atcgcccagc gcgaaggctt gcaccattcg acggtcaacg
     3361 agctgctgcg cctgacgctg ctggagcctg ccatcatcca gagcatcctt gccggacagc
     3421 agcccaagtg catgagcctg ctgtggttcc aacggaaccc acttccgacc gactgggtgg
     3481 cgcagcggga agtggtggcg gggttcgacg cgtgatgcgc tgaactgcat tcagaccacc
     3541 acggcctgac tatcccttgg ctgtcagcgc gccagctgac gatacagctc atcgaactgc
     3601 gccaggctgt cgacaaacgc ccgctcgatg cacttacgcg caggagccgc catctcgtgc
     3661 tggtcgggct cgaggcggat ttgctcgtca tcgcccgcgc tggtcaactc agtcgattgc
     3721 gtcattggat gaccttcctt gcatccttga gcagcaaaag aagttgctgc tcccgcagcg
     3781 gcgatgcaat gaacccgaac cgggtgtaga acctcgccgc ttcttcatcg atgggatggg
     3841 tca
//
LOCUS       DAORCQ010000149         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_19751_length_3765_cov_5.905391, whole genome shotgun sequence.
ACCESSION   DAORCQ010000149 DAORCQ010000000
VERSION     DAORCQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19751_length_3765_cov_5.905391"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..195)
                     /locus_tag="PKI23_11060"
     tRNA            complement(120..195)
                     /locus_tag="PKI23_11060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Thr,seq:ggt)
     gene            complement(236..309)
                     /locus_tag="PKI23_11065"
     tRNA            complement(236..309)
                     /locus_tag="PKI23_11065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(275..277),aa:Gly,seq:tcc)
     gene            complement(361..445)
                     /locus_tag="PKI23_11070"
     tRNA            complement(361..445)
                     /locus_tag="PKI23_11070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Tyr,seq:gta)
     gene            complement(631..2085)
                     /locus_tag="PKI23_11075"
     CDS             complement(631..2085)
                     /locus_tag="PKI23_11075"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HNN37517.1"
                     /translation="MNQSVNPALIGQGDQVGQLRALLDRQRQAFNARPPSDHRERIAR
                     LDALAAGIGRHQQALVDAVMADFGHRSRHETLISEVISALGEIRFTKAKLKGWMKQRR
                     VRPTVQSLGSVGRILYQPKGVIGIMGAWNYPAALCFSPMIGAIAAGNHMLVKPSDLTP
                     RTAEVIRQLVAETFEESYCAVVTGDVGVSVLFSTLPFDHLLYTGGTAVGCQVMQAAAR
                     NLTPVTLEMGGKSPTLIGERFPMARAVQSILMGKLLNAGQTCIAPDYLLLPKARLQEF
                     LEVATAAILERYPRVVDNPDITWIINERHHQRILGLIEDARDKGARIHELNPGHAEIP
                     AGVRVIAPTLLYEVTDQMRVMQEEIFGPLLPIVTYDSLDQAIDYVNRRPRPLALYLFE
                     EDDDRIRRVVNGTISGGVCVNDTMLHIAHQNLPFGGIGPSGLGAYHGFDGFVEFSHKK
                     AVLYQGRFSPAALLKPPYNERALKVLNWVARRLA"
     gene            2246..2893
                     /gene="can"
                     /locus_tag="PKI23_11080"
     CDS             2246..2893
                     /gene="can"
                     /locus_tag="PKI23_11080"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNN37518.1"
                     /translation="MSDLTNLFTNNSRWAASMTAQDPDFFHRLSAQQAPEYLWIGCSD
                     SRVPANEIVGLLPGELFVHRNVANVVGHTDLNCLSVMQYATDVLQVKHIIVCGHYGCG
                     GVQSAVRRNKLGLIDNWLRYVQDVHQKHAGTVNAIDCDQERIRRMCELNVIEQVLNVS
                     QTTVVQDAWDRGQPLTINGWIYGIEDGLLRDLGMGASSLVEATERYHQTIANLLK"
     gene            complement(2909..3337)
                     /locus_tag="PKI23_11085"
     CDS             complement(2909..3337)
                     /locus_tag="PKI23_11085"
                     /inference="COORDINATES: protein motif:HMM:NF022795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HNN37519.1"
                     /translation="MNLLGIGEIIGGVGKIADDLITTDKERLLLAIEEKKIEAGLIQS
                     QIEVNKAEAQHASVFVAGWRPFIGWVGGFGMAYQFLLYPLLTWVWPILIARGTLPVGT
                     TIPPVLDGDVLFAMVSGLLGIAGMRSFDKMKGTDLKTVGR"
     gene            complement(3334..>3765)
                     /locus_tag="PKI23_11090"
     CDS             complement(3334..>3765)
                     /locus_tag="PKI23_11090"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HNN37520.1"
                     /translation="GQTYAGIARNAHPDWPGWRYVDANDLTQVELTQSVRDFYRREFW
                     NRIGGDAITHQTIAETLFDFAVNAGSGTAAKLAQIVVGVVPDGGIGPKTLTALNGCEP
                     ELFALKYALAKVSRYAEICNRDRTQSKFLLGWINRTLKELT"
BASE COUNT          737 a         1222 c         1108 g          698 t
ORIGIN      
        1 gtccccacgt tgacgtgcgg cttcttacgc tcaaacttct ctttcgccat ggcacctttc
       61 tcctcgacca agttcagacc aaaggccacc agcagggggc gaccatgcat cacaaaaact
      121 ggagctcata accggatttg aaccggtgac ctcttcctta ccaaggaagt gctctaccga
      181 ctgagctata tgagcgactc aaaaaccgac aacagcgctg cacatcattt gaaactggag
      241 cgggtagcgg gaatcgaacc cgcgccatca gcttggaagg ctgaggttct accattgaac
      301 catacccgca acacttcttt cgccttgcac gcggccaaca cctgaacccg gcaggcgatc
      361 tggtggaggg gggaggattc gaacctccga aggctgagcc gtcagattta cagtctgatc
      421 cctttgaccg ctcgggaacc cctccaaaat cgagcggcgc attgtcctaa aagattcgcc
      481 aaagatcaac acaaaaacca tctcggcctt tcgcgatgaa tgagtccgtc acgtcgatct
      541 ggcgaccagg cgcatattcg ccgctctgcc agcgcaatga tcatgctttg tgacagcgtg
      601 tgaaacggtt tccgcgctgc ctaacccagc tcaggccaga cgtctggcca cccagttcag
      661 caccttcagc gcacgctcgt tgtagggggg cttcagcagt gcagccggag agaagcgtcc
      721 ctggtagagc accgcctttt tgtgcgagaa ctccacgaaa ccatcgaaac catggtaggc
      781 acccagcccg ctcgggccga tgccgccaaa aggcagattc tggtgggcga tgtgcagcat
      841 ggtgtcgttg acgcagacgc cgcccgagat ggttccgttc accacccgcc tgatcctgtc
      901 gtcatcctcc tcgaacagat agagcgccag cggcctggga cgccggttga cgtagtcgat
      961 ggcctgatcg aggctgtcgt aggtaacgat tggcagcagc gggccgaaga tctcctcctg
     1021 catcaccctc atctgatcgg tcacctcata cagcagggtc ggtgcgatca cccgcacgcc
     1081 ggccggaatc tcggcatgac ccgggttgag ttcgtggatt ctggcaccct tgtcgcgggc
     1141 atcctcgatc agaccgagaa tccgctgatg atggcgctcg ttgatgatcc aagtgatgtc
     1201 gggattgtcg accacccgcg gatagcgctc gagaatcgcc gccgtcgcca cttcgaggaa
     1261 ctcctgcaac cgtgccttcg gcaacagcag atagtcgggt gcgatgcagg tctgaccggc
     1321 attgagcagc ttgcccatca gaatgctctg caccgcccgc gccatcggga accgctcgcc
     1381 gatcaaggtc ggggatttgc cgcccatctc cagcgtgacc ggggtcagat tgcgggccgc
     1441 ggcctgcatc acctgacagc ccactgcggt gccgccagtg tagagcaggt ggtcaaaggg
     1501 cagcgtgctg aacagcaccg agacgccgac atcgccagtg acgaccgcgc agtagctttc
     1561 ctcgaaggtt tccgccacca gttgccggat cacctcggcg gtgcgcggcg tcaggtcgga
     1621 gggtttgacc agcatgtggt tgccggcggc aatggcgccg atcattggcg agaagcagag
     1681 cgctgccgga tagttccagg cacccatgat gccgatcacc cccttgggct gatagagaat
     1741 ccggccgacc gagccgagac tctgtaccgt cggccgcacc cgccgctgtt tcatccagcc
     1801 cttcagcttg gccttggtga agcgaatctc cccgagcgca ctgatcacct cgctgatcag
     1861 cgtctcatgg cgggaacggt ggccaaaatc ggccatcacc gcatcgacca gcgcctgctg
     1921 atgacgaccg atgccagcgg cgagcgcatc gagccgggca atgcgctcgc gatggtccga
     1981 tggtggccgc gcattgaacg cctgccgctg ccgatcgagc agcgcacgca gctgcccgac
     2041 ctgatcgccc tgcccgatga gggctggatt cacgctctga ttcatctgca tggctcctgt
     2101 aacaggcaat tgttccaatc aaccccaaac acctggcaac gggaactcag ccctggcgcg
     2161 gactcaagcg gccgattttg ccctagaatg ccgcgccagc ctcatcgatg acaacctcgc
     2221 cgttcaaccg atccaggagt tccccatgtc cgatctgacc aacctgttca ccaacaacag
     2281 ccgctgggca gcctcgatga ccgcacagga tccagacttc ttccatcgtc tctccgccca
     2341 gcaggcgccg gaatatctct ggatcggctg ctccgacagc cgggtgccag ccaatgagat
     2401 cgtcgggctg ctgccgggcg agctcttcgt gcaccgcaat gtcgccaatg tggtgggcca
     2461 cactgacctc aactgcctgt cggtgatgca gtacgccacc gacgtgctgc aggtgaagca
     2521 catcatcgtc tgcggccact atggctgtgg cggcgtgcag tcggccgtgc gccggaacaa
     2581 gctcggtctg atcgacaact ggctgcgcta tgtgcaggat gtccatcaga agcacgccgg
     2641 cacggtgaat gcgatcgact gcgatcagga gcgcattcgc cggatgtgcg aactcaacgt
     2701 catcgaacag gtgctgaatg tcagccagac caccgtggtg caggatgcct gggaccgcgg
     2761 ccagccgctg accatcaacg gctggatcta cggcatcgag gatggcctgc tgcgcgacct
     2821 cggcatgggc gcttccagcc tggtcgaggc gaccgagcgc taccaccaga ccatcgccaa
     2881 cctgctgaag taggcgcggc cgatcgactc agcgaccgac cgtcttcagg tcggtcccct
     2941 tcatcttgtc gaagctgcgc atcccggcga tgccgagcag gccgctgacc attgcgaaca
     3001 gcacatcccc atcgagcact ggcgggatgg tggtgccgac cggcagcgtg ccgcgtgcga
     3061 tcaggatcgg ccagacccac gtcagcagcg gatagagcag aaactgatag gccatgccga
     3121 agccgccgac ccagccgatg aacggccgcc agccggccac gaagacgctg gcgtgctgcg
     3181 cctcggcctt gttgacctcg atctgcgact ggatcagccc ggcttcgatc ttcttctcct
     3241 cgatcgcaag tagaagccgc tccttatcgg tggtgatcag gtcatcggcg atcttgccga
     3301 ccccgccgat gatttcgccg atgcccagca ggttcatgtc agctccttca gcgtgcggtt
     3361 gatccagccc agcaggaatt tgctctgcgt ccggtcgcgg ttgcagattt ccgcatagcg
     3421 cgacaccttg gccagcgcgt acttgagcgc gaacagctct ggctcacagc cgttgagtgc
     3481 ggtcagcgtc ttgggaccga tgccgccatc cggcaccacc cccaccacga tctgcgccaa
     3541 cttggcagcc gtgccgctgc cggcgttgac ggcaaagtcg aacagggtct cggcaatggt
     3601 ctgatgggtg atggcgtctc cgccgatgcg gttccagaat tcgcggcggt agaagtcacg
     3661 cacgctctgg gtcagctcga cctgagtcag gtcattggca tcaacgtagc gccagcccgg
     3721 ccagtcgggg tgggcattgc gggcgatgcc ggcgtaggtc tgccc
//
LOCUS       DAORCQ010000150         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_20000_length_3738_cov_6.524301, whole genome shotgun sequence.
ACCESSION   DAORCQ010000150 DAORCQ010000000
VERSION     DAORCQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20000_length_3738_cov_6.524301"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..672)
                     /locus_tag="PKI23_11095"
     CDS             complement(166..672)
                     /locus_tag="PKI23_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNN37521.1"
                     /translation="MEPVDAATGSSATQAIRALLRHPELSRLGCAHPLRCAANQTVIQ
                     EGTQCGKLYLVDSGQLRILNGVLLEGARQIQPGVADVGPGELIGELAMFDGAPHAASV
                     VTLTTAELTEIDSEILLRFMDRHPELGYPVMKALIEALIGRFRRTSRKVSSLFAWGLK
                     AHRIDQHL"
     gene            complement(659..1291)
                     /gene="crp"
                     /locus_tag="PKI23_11100"
     CDS             complement(659..1291)
                     /gene="crp"
                     /locus_tag="PKI23_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HNN37522.1"
                     /translation="MSSRLQPAIPGLDQFLAQCHRRRYTKNATIIKQGDPCNTLYYLC
                     KGSVTVALEEQEGKEMVVCYLNGGDFFGEIGLFTTEPARSAMVRARTECEVAEISYGK
                     FQQLIEQHPAMLHTLATQLANRLRATTRKASNLTFLDVTGRVARTLLDLCKQPDAMSH
                     PDGMQIKVTRQEIGRMVGCSREMVGRVLKDLEQQGLVTARGKTMVVYGTR"
     gene            1631..1876
                     /gene="rpsP"
                     /locus_tag="PKI23_11105"
     CDS             1631..1876
                     /gene="rpsP"
                     /locus_tag="PKI23_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNN37523.1"
                     /translation="MVKIRLARSGAKKRPFYHLTVTDSRNKRDGRFCERVGFFNPVAR
                     GQEVKLKVELERIDYWLSQGAQPTDRARKLIQQARQQ"
     gene            1900..2424
                     /gene="rimM"
                     /locus_tag="PKI23_11110"
     CDS             1900..2424
                     /gene="rimM"
                     /locus_tag="PKI23_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160955.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNN37524.1"
                     /translation="MKADEWVVLGKVSTAFGIKGWVKIHSYTEPPENLLHYPQLWRVT
                     AQGRQKIDLVEAKVQGRGLIGRFADCDDRDQALGYAGDELAVPATALPELEAGDYYWH
                     QLEGMRVINRQGELLGWVDRLFETGANDVVVVRPGEGSIDERERLIPYLPERVVIRVD
                     LEQAELHVDWPADF"
     gene            2450..3226
                     /gene="trmD"
                     /locus_tag="PKI23_11115"
     CDS             2450..3226
                     /gene="trmD"
                     /locus_tag="PKI23_11115"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNN37525.1"
                     /translation="MWFGVVTLFPELLQPVLTLGVTGRAIVQGLLQVECWNPRQFTSD
                     LHQTVDDRPYGGGPGMVMKVAPLQAAIGAARAVAPKPPSVVYLSPQGRRIDQSMLERC
                     AGMEAVLLLAGRYEGVDERLIDLEVDEEWSLGDFVLSGGEIAAMALIDGVARLLPGAL
                     GHAQSARCDSFSTGLLDHPHYTRPECHQGAAAPQVLLSGDHGAIERWRRQQALVRSWL
                     RRPELLKECRLSLAELEFLERFAAAKQSGREPTGTGVSPL"
     gene            3239..3670
                     /gene="rplS"
                     /locus_tag="PKI23_11120"
     CDS             3239..3670
                     /gene="rplS"
                     /locus_tag="PKI23_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNN37526.1"
                     /translation="MSNKNALITQIEAEQMQRVLPAFAPGDTVVVQVRVLESGKDSST
                     AKKGKTAAKAPKKDSSKVDAARERFQAFEGVVIAKHNRGLNSSFVVRKISHGVAVERT
                     FQSYSPMLGEIEVKRRGDVRKAKLYYLRDRSGKSARIREKV"
BASE COUNT          708 a         1062 c         1261 g          707 t
ORIGIN      
        1 tggcatcgaa cggctgcagg aggcggccaa gcacggcttc acccgcgcca tcgtgccgag
       61 tgccaacgca ccccggcagc cgatcgccgg catgacggtg accgcggtcg agcgggtggc
      121 cgatgccctg gagagcttct gaaaagcagt gctgccaagg cggcgctaca gatgctgatc
      181 gatgcggtgc gccttgagtc cccaggcaaa aagcgaggag accttgcggc tggtccggcg
      241 aaagcggccg atcagggcct cgatcagcgc cttcatcacc gggtagccca gctcggggtg
      301 gcggtccatg aagcgcagca ggatttcact gtcgatctcg gtcagctcgg cagtcgtgag
      361 ggtgacgacc gaggcggcat ggggcgcgcc atcgaacatc gccagttcac cgatcagctc
      421 gccgggaccg acgtcggcca cacccggctg aatctgtcgg gcaccctcca gcagcactcc
      481 attcagaatc cgcagctggc cgctgtcgac cagataaagc tttccacact gcgtgccctc
      541 ctggatcacc gtctggttgg ctgcacagcg cagtggatgc gcgcagccga gccggctcag
      601 ttcggggtgg cgcagcagtg cgcggatggc ctgggttgcc gaactgccag tcgccgcatc
      661 aacgggttcc atacaccacc atcgtcttgc cgcgcgcagt gaccagaccc tgctgctcca
      721 gatccttgag cacacgtcct accatctccc gcgagcagcc caccatgcgg ccgatctcct
      781 gccgggtgac cttgatctgc atgccatcag gatgcgacat cgcatccggc tgcttgcaca
      841 ggtcgagcag cgtgcgcgcc acgcgcccgg tcacatcgag aaaggtcagg ttgctcgcct
      901 tgcgggtggt ggcgcgcagc cgattggcca gttgcgtggc gagggtgtgc agcatggctg
      961 gatgctgttc gatcagttgc tggaacttgc cgtagctgat ttcggcgacc tcgcattcag
     1021 tgcgggcccg aaccatcgcc gaacgcgctg gttcggtggt gaagaggccg atctcgccga
     1081 aaaagtcacc accgttgaga taacagacca ccatctcctt gccctcctgc tcttcgagtg
     1141 cgacagtcac cgagcctttg cagagatagt agagcgtatt gcaggggtcg ccttgcttga
     1201 tgatggtggc gtttttcgtg taacgacgac ggtgacactg ggcgagaaac tgatccagcc
     1261 ccggaatggc tggctggagt cgactgctga tgccgagcgc tctgggggag tttcgctgta
     1321 gttcgctcat ggttcgatct ggccacgatg gggatgacgc aactggaagg attgcaggtt
     1381 gattgcttga aagtatggtt gacagagtcg atttccgcca acgagccggc ctggatctcg
     1441 acctcggatt tcactggcga acgggcggga gtccgcagga tttgaaatgg gatgaaaatg
     1501 caatagaatg gccccccttt taaggtcaag gccagcaaag caggcgctgt tgggcagtgc
     1561 ggtgcccgcg gttgcggttg gttgggctca ataaactctc cagggcatcc tccaggaagc
     1621 gatatcgaca atggtaaaga ttcgacttgc acgaagcggc gccaagaagc gtccctttta
     1681 tcacctgacc gtgaccgaca gtcgcaacaa gcgcgatggc cgcttctgtg agcgggtggg
     1741 tttcttcaac ccggtggcgc gtgggcagga ggtcaagctc aaggttgaac tggagaggat
     1801 cgactactgg ctgtcacagg gcgcccagcc gaccgaccgt gccaggaagc tgatccagca
     1861 ggcacggcag cagtaagccg atccagagtc ttcgcgcggg tgaaggccga tgaatgggtg
     1921 gtgctcggca aggtcagcac cgcattcggt atcaaggggt gggtcaagat tcactcctac
     1981 accgaaccgc cggaaaacct tctgcattac ccccagttgt ggcgggtgac cgcgcagggc
     2041 cggcagaaga tcgatctggt cgaggccaag gtgcaggggc gtggcctgat cggtcgattc
     2101 gccgattgcg acgaccgtga tcaggcgctg ggttatgcgg gggatgagtt ggcggtgccg
     2161 gcaacggccc tgccagagct ggaagccgga gactactact ggcaccagct tgaagggatg
     2221 cgggtgatca accggcaagg cgaactgctg ggttgggtcg atcgactgtt cgagaccggt
     2281 gccaacgatg tggtcgtggt ccgtcccggt gaaggcagca tcgatgagcg ggagcgattg
     2341 atcccctacc tccctgagcg tgtcgtcatc cgggtggatc tggagcaggc cgaactgcat
     2401 gtcgactggc ctgccgattt ttgagagttg aacaggcggt tcaagagcaa tgtggtttgg
     2461 cgtggtgacg ctctttccgg aactgctgca accggtgctg accctcggcg tgaccggccg
     2521 ggccattgtc caggggctgt tgcaggtgga gtgctggaat ccacggcagt tcaccagcga
     2581 tctgcaccag acggtcgatg accggcccta tggcggtgga cctggcatgg tgatgaaggt
     2641 ggcgccgctg caggccgcga tcggcgccgc acgtgccgtg gcaccgaagc caccgagcgt
     2701 ggtctatctt tcgccgcagg ggcgcaggat cgaccagagc atgctggagc gctgtgcagg
     2761 gatggaggcg gtgctgctgc ttgctggccg ctatgaaggt gtcgatgagc ggttgatcga
     2821 cctcgaggtc gatgaagagt ggtcgttggg tgactttgtg ttgagcggtg gggaaatcgc
     2881 cgcgatggcg ctgatcgacg gtgtggcccg gctgttgccg ggtgcgttgg ggcatgcgca
     2941 gtctgcacgc tgtgactcat tcagcaccgg cctgctggac catccgcact atacccgtcc
     3001 ggagtgtcat cagggcgctg cggcacccca ggtgttgttg agcggtgacc atggagcgat
     3061 cgagcgctgg cgacggcaac aggcattggt gcgaagctgg ctacgccggc cggagctgtt
     3121 gaaagagtgc aggctcagcc tggcggaact ggaatttctg gaacgatttg ccgcggcgaa
     3181 acagtctggc cgcgaaccca ctggtacggg cgtcagccca ctttgaggat gaacagcgat
     3241 gagcaacaag aacgcattga tcacccagat cgaagccgaa cagatgcaga gagtcctgcc
     3301 ggcgtttgcg ccgggcgaca ccgtcgtggt gcaggtcagg gtgctggaga gtggcaagga
     3361 ctcttcgacc gccaaaaagg gcaaaaccgc cgccaaggcg cccaaaaagg attcgagcaa
     3421 ggtcgatgcg gcccgcgagc gctttcaggc ctttgaaggg gtggtgatcg ccaagcacaa
     3481 ccggggcctg aactcctctt tcgtggtgcg caaaatctcc catggcgtgg cggtcgagcg
     3541 gacctttcag agctacagcc cgatgctcgg cgagatcgaa gtgaagcgtc gcggcgatgt
     3601 ccgcaaggcc aagctgtact acctgcgtga ccgctcgggc aaatcggccc gtatccgcga
     3661 gaaggtctga tcctgttcgc tgcctgactt ctcctgtcag gcgcagccaa gcggtactgg
     3721 tttttgttcc gtgtgccc
//
LOCUS       DAORCQ010000151         3660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_20688_length_3660_cov_5.611096, whole genome shotgun sequence.
ACCESSION   DAORCQ010000151 DAORCQ010000000
VERSION     DAORCQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20688_length_3660_cov_5.611096"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PKI23_11125"
     CDS             complement(<1..1085)
                     /locus_tag="PKI23_11125"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HNN37527.1"
                     /translation="MQRHFLFLQGMPCDFMRRVGDALQMRGHRVSRINLCFADWLFWH
                     DRRALSYRGRLAQWPAFLREFLQRQAVTDLVLLGEQRKYHREAVALAQSLNIRVMVTD
                     FGYFRPDWITLEPNGMGAQSTMPKDPAVIRQLAAELPEIDFTPCHLDSSLRMALGDLA
                     GSFGNLLGWMGYPHYRQSDERPHPLLYFPAMGWSLLRERSRTRQAQRDFARLQRRGRP
                     FFVFALQLDHDFQIRAYSPFAGMEQALDQVLHSFARHAPEQCELLIKTHPWDPGLIRW
                     SSVIARQARALALGDRVHLLQGGSLDQMMADAQGMVTVNSTAGLQALRLQKPVQVLGS
                     AVYDIAGLTHQAGLDRFWMAPGAPDPLL"
     gene            complement(1107..2153)
                     /locus_tag="PKI23_11130"
     CDS             complement(1107..2153)
                     /locus_tag="PKI23_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNN37528.1"
                     /translation="MRAEAEAAAVPPCAAHYPGVRVAVTGASGFIGGQLARRLVQLDA
                     RVTLLVRDPERLDPLLRQRCELVPGDLLDRAAVEQLLHGATLVFHCAANVATWDRWPA
                     YQAANVQGVEMLLSVLAANPAALRRLVHLSTMDVYGFPAQPASESAPLRPLPFGYGES
                     KRQGELRVRQQCERHGLGHVVIRPGNIIGPGSPFIRRIGQALRSGVMLEVNRGAAHAG
                     LMDVDNLIDLLIWAAVAPGADQQLLNARDPWAVSWGEFLRDLRAALPVRGRLLNLDRR
                     TAMGLARLCGALHRRLHLRGEPLLHPLIVEIFGRSCGHSIETLNALAAPIGRIDYQQS
                     LRRSVDWLLATAGG"
     gene            complement(2150..3295)
                     /locus_tag="PKI23_11135"
     CDS             complement(2150..3295)
                     /locus_tag="PKI23_11135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNN37529.1"
                     /translation="MIRVRLHDSIDELEPAQWDALLAGRSITFSHAFWRIIEQSQLND
                     FHYRHLLFVDDDEQPVGLATFYRVTTDIAIFAPPWLRRALGAVRRMFPNFLKLRMLEC
                     GTPIILNSPPALLHPDLPADEVIEVLHRTLMRHARDEGVLLLVLRDFERNAQPWLGPL
                     RRQGYHLVDGLPNSYLDIRWRDVADYRAALKSYYRSKINNHLRKNAELQVWHDLIDDF
                     APLADALCRQWLTVHARANEFQREVLTPTFYRELALRMGGRAKVLRLFRGRDWIGHAL
                     LLHDGDTLRWLYFGRDEPVNDSLYLYVTQAVIQTAIELGVARLELGLTTYPIKQDAGA
                     RLEPIQFAVRCRWGLFNPLVGRLYGLFNKPARIQDRQVFKAGQEPQP"
     gene            complement(3292..>3660)
                     /locus_tag="PKI23_11140"
     CDS             complement(3292..>3660)
                     /locus_tag="PKI23_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37530.1"
                     /translation="DQVDEVHLLTSLTGFEALLRGKPVTCHGQPFYAGWGLTEDVTPH
                     ARRGRRRSLDELVAAALILYPRYLSPHDGSVITPEAAVDQLVALRQQGGDRSGWRRRL
                     LRPLLRALAHIEDGARRRQG"
BASE COUNT          678 a         1292 c         1143 g          547 t
ORIGIN      
        1 agcagcggat cgggcgcgcc gggcgccatc cagaagcgat cgagccctgc ctgatgggtc
       61 aacccggcaa tgtcgtagac cgcgctgccc agcacctgta ccggcttctg cagccgcagc
      121 gcctgcaggc cggcggtgct gttgaccgtc accatgccct gtgcatcggc catcatctga
      181 tcgagtgagc ccccttgcag cagatggacg cggtcgccca gcgcgagcgc cctcgcctgc
      241 cgggcaatca cactgctcca gcgaatcagc cccggatccc aggggtgggt cttgatcagc
      301 agctcgcact gctccggcgc atggcgggca aaggagtgca gcacctgatc cagcgcctgc
      361 tccatgccgg caaagggcga ataggcgcga atctgaaaat cgtggtcgag ttgcagcgcg
      421 aagacgaaga acggccgtcc acgccgctgc aaccgcgcaa aatcccgttg cgcctgccgg
      481 gttctcgagc gctcgcgcag cagactccag cccatcgccg gaaagtagag cagcggatgg
      541 ggccgctcat ccgactggcg atagtgtggg tagcccatcc agcccagcag gttgccaaag
      601 ctgccggcca gatcgccgag cgccatgcgc aggctgctgt cgagatggca tggcgtgaag
      661 tcgatttcgg gcagttcagc ggccagttgc ctgatcacgg ccggatcttt gggcatcgtc
      721 gactgcgcgc ccatgccgtt gggctccagc gtgatccagt ccggccgaaa gtagccgaag
      781 tcagtcacca tgacgcgaat gttcagcgac tgcgccagcg ccactgcctc gcggtggtat
      841 ttgcgctgct cgcccagcag caccagatcg gtcaccgcct gccgctgcag gaactcgcgc
      901 agaaacgccg gccactgcgc cagccgccca cgatagctga gcgcacgccg atcatgccag
      961 aacagccagt cggcaaagca gaggttgatg cggctgaccc ggtgaccccg catctgcaat
     1021 gcatcgccga cccggcgcat gaagtcgcac ggcatcccct gcaggaacag aaagtggcgc
     1081 tgcatcgatg gctccatccg ccggactcag ccgcctgccg ttgccagcag ccagtcgacc
     1141 gaccgccgca ggctctgttg atagtcgatc cgtccgatcg gcgccgcgag ggcgttcagc
     1201 gtctcgatcg aatggccgca gctcctgccg aaaatctcga cgatcagcgg atgcagcagc
     1261 ggctcgccac gcagatgcaa ccgccggtgc agcgcaccgc acagccgcgc cagccccatc
     1321 gccgtgcgcc gatccagatt gagcagtcgc ccccgcaccg gcaacgcggc gcgcagatcg
     1381 cgcagaaact ctccccagct caccgcccac ggatcacggg cattgagcag ttgctggtcg
     1441 gcacctggtg caaccgccgc ccagatcagc agatcgatca ggttgtcgac atccatcagc
     1501 ccggcatggg ccgccccccg gttcacctcc agcatcacgc ccgacctcaa tgcctggccg
     1561 atgcggcgga tgaaggggct gccggggccg atgatgttgc ccggtctgat caccacatgg
     1621 cccagcccat gccgttcaca ctgctggcga acccgcagct cgccctgccg cttgctctcg
     1681 ccatagccaa aaggcagcgg tcgcagtggc gcggactcgc tggccggctg cgcggggaag
     1741 ccgtagacat ccatggtgga gagatggacc agccgcctca gtgccgcagg gttcgccgcc
     1801 agcacgctca gcaacatctc gaccccctgc acattggcgg cctggtaggc cggccagcga
     1861 tcccaggtcg ccacattggc ggcgcagtgg aacaccaagg tcgcgccatg cagcagctgc
     1921 tcgaccgctg cacggtcgag caaatcgccc ggcaccagtt cgcaccgctg ccgcagcagc
     1981 gggtcgagcc gctccgggtc acgcaccagc agcgtcaccc gggcatcgag ctgcaccagc
     2041 cgccgggcca gctgcccacc gatgaagccg ctggcgccgg tcaccgccac ccgcacgccc
     2101 ggatagtggg cagcgcatgg cggcacggca gcggcctcgg cctccgccct catggctgtg
     2161 gctcctgccc ggccttgaac acctgtctgt cctggattct ggccggtttg ttgaacagcc
     2221 catagagcct gccgaccagc ggattgaaca gcccccagcg gcagcgcacc gcgaactgga
     2281 tcggctcgag ccgcgccccg gcatcctgct tgatcggata ggtggtcagc cccagctcca
     2341 gacgcgccac ccccagctcg atggcggtct ggatcaccgc ctgggtgacg tagagataga
     2401 ggctgtcatt caccggttca tcacggccga agtagagcca gcgcagcgtg tcgccatcgt
     2461 gcagcagcag cgcatggccg atccagtccc gcccgcggaa cagacgcagc accttggccc
     2521 gtccacccat ccgcagcgcc agttcgcggt agaaggtggg cgtcagcacc tcgcgctgga
     2581 actcgttggc acgcgcatga acggtgagcc actgacggca gagcgcatcg gccaggggcg
     2641 caaagtcgtc gatcagatcg tgccagacct gcaactcggc attcttgcgc agatggttgt
     2701 tgatcttgct gcgatagtag ctcttcagcg ccgcccggta gtcggcgaca tcgcgccagc
     2761 ggatatcgag gtagctgttt ggcagcccat cgaccagatg gtaaccctgc cgtcgcaacg
     2821 ggccgagcca cggctgcgca ttgcgctcga aatcgcgcag aaccagcagc agcacccctt
     2881 cgtcacgtgc atggcgcatc agcgtccggt gcagcacctc gatcacctca tcggctggca
     2941 gatcggggtg gagcagcgcc ggcgggctgt tgaggatgat cggcgtgcca cactccagca
     3001 tccgcagctt gagaaagttg gggaacatcc gccgcaccgc acccagcgcg cgccgcagcc
     3061 agggcggcgc aaagatggcg atgtcggtgg tcacccgata gaaggtcgcc aggcccaccg
     3121 gctgctcatc gtcatccacg aacagcagat ggcggtagtg aaagtcattg agctgcgact
     3181 gctcgatgat gcgccagaag gcgtgcgaga aggtgatcga gcgcccggcc agcagcgcat
     3241 cccactgcgc cggctccagc tcatcgatcg agtcgtgcag ccgcacccgg atcatccctg
     3301 cctccttctg gcgccatcct cgatatgcgc cagagcgcgc agcagcggcc gcagcaaccg
     3361 ccgtcgccaa ccggatcggt caccaccctg ctgccgcagc gcaaccagtt gatccaccgc
     3421 tgcttcgggc gtgatcacgc tgccatcatg cgggctcaaa taccggggat agagaatcag
     3481 tgcagcggcc accagctcat ccagcgaccg gcgccggccg cgccgcgcat gaggcgtgac
     3541 atcctcggtc agaccccagc cggcatagaa gggctggcca tgacaggtga ccggcttgcc
     3601 acgcagcagc gcctcgaagc cggtcagcga ggtgagcaga tgcacctcat cgacctgatc
//
LOCUS       DAORCQ010000152         3655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_20734_length_3655_cov_6.101389, whole genome shotgun sequence.
ACCESSION   DAORCQ010000152 DAORCQ010000000
VERSION     DAORCQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20734_length_3655_cov_6.101389"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..303)
                     /locus_tag="PKI23_11145"
     tRNA            complement(214..303)
                     /locus_tag="PKI23_11145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(267..269),aa:Ser,seq:tga)
     gene            complement(317..877)
                     /locus_tag="PKI23_11150"
     CDS             complement(317..877)
                     /locus_tag="PKI23_11150"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNN37531.1"
                     /translation="MVRLEGFAPIADARARLLILGSMPGGHSLRLGEYYAHPRNRFWP
                     IMAELCGFDAALAYPQRLEALKSSGIALWDVLHACRREGSADAKIDDASLELNDIAGF
                     LRQHPRIHAILFNGAKAEQCFLRHVRSTLDETSLRCLRLPSTSPAHATLGQQQKLAAW
                     RSALSQFDPGGLACEQSPIQFPGRLR"
     gene            complement(888..1745)
                     /gene="speE"
                     /locus_tag="PKI23_11155"
     CDS             complement(888..1745)
                     /gene="speE"
                     /locus_tag="PKI23_11155"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNN37532.1"
                     /translation="MSNGDAWVIERFGDEGSAIGLAVEAKLHEEQSEFQKIEIYQSRS
                     YGKVMLIDGCFMLSERDHFIYHEMITHPALFSHPAPRRVAIIGGGDCGTLTEVLKHRG
                     VEQAWQIEIDERVTRVSEQYFPELCGSNGDPRAQLLFADGIAWMRERPADSLDVIIID
                     STDPVGPAAGLFHPDFYRDCHRALGAQGVLVQQSESPLFHAGSIIRGVHDGLAQVGFS
                     SRRTLTFPLTIYPSGWWSVTLGGHGVTLAETGPARFDELGIDTRYYTPQIHQAAGVLP
                     RFVREALAQ"
     gene            1968..>3655
                     /gene="speA"
                     /locus_tag="PKI23_11160"
     CDS             1968..>3655
                     /gene="speA"
                     /locus_tag="PKI23_11160"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNN37533.1"
                     /translation="MAHDHTMTAAAHFRVARWGEGYFDIDAQGEVVVAPRGARAPQRF
                     KLTAIADRVRAQGLNYPILLRFTDIIADRLTALSEAFEQALARSGHASGYRAIYPIKV
                     NQQREVVEAMVAAQKQRGYVGLEAGSKPELLIVLANAEPGCTRIVCNGYKDRDFVRLA
                     LLGEKLGHCVYLVIESLSEVELILDEAARLGVRPRLGVRVRLATVAKGNWQNTGGEKS
                     KFGLSAAQILTLVERLQRAGRLDCLEMLHSHIGSQIADLEDIRRGSQEAARFYGELHR
                     LGAAVQVVNLGGGLSVDYDGSFSSSYFSMNYRFAEYAGAIIEGITVECARRQLPFPQL
                     MSESGRALTAHHALLLTDVADVERPVQEVAPAATLLAQWAEVSPVHRRLVECHQRLEA
                     GDLDHARNALQWMEGWLDELETLFAAGEVSLRQRAEAEQLHAINCRKIAALIAAADIP
                     DDGLLAALREKLADRLYVNFSLFQSMPDIWGIDQIFPVLPLSGLDQPLQRRALIHDIT
                     CDSDGRLDRYVTAERTTLALPEFDPHQPPLIGFFLLGAYQETLGDLHNLFGRVDS"
BASE COUNT          644 a         1189 c         1209 g          613 t
ORIGIN      
        1 aaagggtgag tccagtggtc agccagacct gccatgccag tgccatcgtc acctcgggtg
       61 tgttgtggaa gtcagtcgga gtgattcatc gcctggcctg catcgcctgc gctgccatgc
      121 ggcatgggcg gatacaggca cgagtcgagc caacttcgtt gcgtgatgac gaaaaccaag
      181 ctggcggcgc agtgggcagg agacgaaggc ggatggcgga gggggtggga tttgaaccca
      241 cgaagggcgt taacccttgc cggttttcaa gaccggtgca atcaaccgct ctgccacccc
      301 tccggcggcc gccatcttac cgcagccgac cggggaattg aattggactt tgttcgcagg
      361 ccaagccacc cggatcgaac tggctgagtg ccgaccgcca ggcagccagc ttctgctgct
      421 ggccgagggt ggcatgggcc ggactggtcg aaggcagccg caggcagcgg agcgatgttt
      481 catccagagt ggaccgcaca tggcgcagaa agcactgctc ggccttggca ccattgaaga
      541 gaatggcgtg gattcgcgga tgttggcgca gaaagccggc aatgtcgttg agctcgagcg
      601 aagcatcgtc gatcttcgca tcggcgctgc cttcgcggcg gcaggcgtgg agcacatccc
      661 acagcgcgat gccggacgac ttgagcgcct cgagtcgctg cgggtaggcg agcgctgcgt
      721 caaaaccgca gagttcggcc atgatcggcc agaaccggtt gcgcggatgg gcgtagtatt
      781 cgcccagccg cagcgagtgg ccgcccggca tgctgccgag aatcagcaac cgcgcccgcg
      841 catccgcgat cggggcaaac ccctccagcc ggaccatcga cggccggcta ctgcgccagc
      901 gcctcacgca cgaagcgtgg cagcacaccg gccgcctggt ggatctgcgg cgtgtagtag
      961 cgggtgtcga tccccagctc gtcgaaccgg gccggccccg tttccgccag ggtcacccca
     1021 tgcccgccga gggtgacgct ccaccagccc gaagggtaga tggtgagcgg aaaggtcaag
     1081 gtacggcggc tgctgaagcc gacctgcgcc aatccgtcgt gcacccccct gatgatgctg
     1141 ccggcgtgga acagcggcga ttcgctctgc tgcaccagca cgccctgggc acccagcgcc
     1201 cggtggcagt cgcggtagaa gtcggggtgg aacagcccgg cagcggggcc gaccggatcg
     1261 gtcgagtcga tgatgatcac gtcgagcgaa tcggccgggc gttcacgcat ccaggcgatg
     1321 ccgtcggcaa acagcagttg ggcgcgcggg tcgccattgc tgccacacag ctcgggaaag
     1381 tactgctccg acacccgggt gacccgctcg tcgatctcga tctgccaggc ctgctcgacg
     1441 ccacggtgct tcagcacctc ggtgagggtg ccgcagtcgc cgccgccgat gatggccacc
     1501 cgtcggggcg cgggatggct gaagagcgcc ggatgggtga tcatctcgtg gtagatgaag
     1561 tggtcacgct cgctgagcat gaagcagcca tcgatcagca tcaccttgcc gtagctgcgg
     1621 ctttggtaga tctcgatctt ctggaattcg ctctgctcct cgtgcagctt ggcctcgacc
     1681 gcaagcccga tcgccgagcc ctcgtcacca aaccgctcga tcacccaggc gtcgccgttg
     1741 ctcatcgatt gtcctcgttt gtcatgtcac tgaagtaagg cagtcattac cctgtgcccg
     1801 atcacagcgg gacgatcgac aggcaaagtg ctctggctgg cagaaaagag cggctattat
     1861 agtgagtccg cccgcagcac gcgctgccga gtgcagtgca gccgtccatt ttaccgacca
     1921 ccacccatcg tcgacctgcc gttcgcgcat cacagaggag tatcccgatg gcgcatgacc
     1981 acaccatgac cgccgccgcc cattttcgcg tcgcccgctg gggcgagggc tacttcgaca
     2041 tcgatgcaca aggggaggtg gtggtggcgc cccggggcgc acgggcaccg cagcggttca
     2101 agctgaccgc catcgccgac cgggtgcggg cccaggggct caactacccg attttgctgc
     2161 gcttcaccga catcatcgcc gaccggctga cggcactgtc ggaggcgttc gagcaggccc
     2221 tcgcgcgcag cggccatgcc agcggctacc gggccatcta tccgatcaag gtgaaccagc
     2281 agcgcgaggt ggtcgaggcg atggttgccg cgcagaagca gcgtggctat gtcggcctcg
     2341 aggccggcag caagccggaa ctgctgatcg tgctggccaa tgccgaaccg ggctgcaccc
     2401 gcatcgtctg caacggttac aaggaccgtg acttcgtccg gctggcgctg ctgggcgaaa
     2461 agctcggcca ctgcgtctat ctggtgatcg agagcctgag cgaggtcgag ctgattctcg
     2521 acgaagccgc acgcctcggt gtgcggcctc gcctcggcgt gcgggtgcgg ctggccacgg
     2581 tggccaaggg caactggcag aacaccggcg gcgagaagtc gaagtttggc ctgtcggcag
     2641 cgcagattct gaccctcgtc gagcggctgc aacgcgccgg acggctcgac tgcctggaga
     2701 tgctccacag ccacatcggc tcgcagatcg ccgacctcga ggacattcgc cgcggcagcc
     2761 aggaggcggc gcgcttctac ggcgaactgc accggctggg cgccgccgtg caggtggtca
     2821 acctcggcgg tggcttgagc gtcgactacg atggcagctt ctccagcagc tacttctcga
     2881 tgaactaccg ctttgccgaa tatgccggcg cgatcatcga aggcatcacg gtcgaatgcg
     2941 cccgccggca actgccgttc ccgcaactga tgagcgaatc tggacgggcg ctcaccgcgc
     3001 accatgccct gttgctgacc gatgtcgccg acgtcgaacg gccggtgcag gaagtcgcgc
     3061 cggccgccac gctgctggcg cagtgggccg aggtctcgcc ggtgcaccgg cggctggtgg
     3121 agtgccatca gcggctcgaa gcgggggatc tcgaccatgc gcgcaacgcc ctgcagtgga
     3181 tggagggatg gctggacgaa ctggagacgc tgttcgccgc cggcgaggtc tcgctgcggc
     3241 agcgggccga ggccgagcag ttgcatgcga tcaactgccg caagatcgcc gccctgatcg
     3301 ctgcggcaga cattcccgac gatggcctgc tggccgcact gcgcgagaag ctggccgacc
     3361 ggctctatgt caacttctca ctgtttcagt cgatgccgga catctggggc atcgaccaga
     3421 tctttccggt gctgccactg agcggcctcg atcagcctct gcaacgccgc gcactgattc
     3481 atgacatcac ctgcgactcc gatggccggc tcgaccgcta tgtcacggcc gaacgcacca
     3541 cgctggcgct gccggagttc gacccgcacc aaccaccgct gatcggattc tttctgctgg
     3601 gtgcctatca ggagaccctc ggcgatctgc acaacctgtt cggccgggtc gactc
//
LOCUS       DAORCQ010000153         3593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_21326_length_3593_cov_5.962125, whole genome shotgun sequence.
ACCESSION   DAORCQ010000153 DAORCQ010000000
VERSION     DAORCQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21326_length_3593_cov_5.962125"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKI23_11165"
     CDS             complement(<1..477)
                     /locus_tag="PKI23_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNN37534.1"
                     /translation="MFKRLRGMFSNDLSIDLGTANTLIYVRGKGIVLDEPSVVAIRIQ
                     NGQKTVCAVGSAAKRMLGRTPGNITAIRPLKDGVIADFQVTEKMLQHFIAKTHANSFI
                     RPSPRVLISIPCGSTQVERRAIRESAIGAGARDVRLIEEPMAAAIGAGMPVEEASGS"
     gene            691..978
                     /gene="gatC"
                     /locus_tag="PKI23_11170"
     CDS             691..978
                     /gene="gatC"
                     /locus_tag="PKI23_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNN37535.1"
                     /translation="MKMKPEEIQRIAQLARLRIDVDHHAEVGVRLSAILDLVQQMHQI
                     DTQNIEPLAHPLNVTQRLRGDEVTETDQRERFQQLAPLTEDGLYLVPKVIE"
     gene            1031..2500
                     /gene="gatA"
                     /locus_tag="PKI23_11175"
     CDS             1031..2500
                     /gene="gatA"
                     /locus_tag="PKI23_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNN37536.1"
                     /translation="MHNLTLTQLAHGLDAGEFSSVELTRHFLQRIDSLDGHYNSFITR
                     TAEQALDAAAAADARRARGEAGLGAGLPLAHKDIFCTDGVRTTCGSRMLGNFVSPYDA
                     TVVERFRAAGWVCLGKTNMDEFAMGSSNETSYFGPVRNPWDRSRVPGGSSGGSAAAVA
                     ARLAPGATASDTGGSIRQPAALCGISGLKPTYGRVSRYGMIAFASSLDQGGPMARSAA
                     DLSLMLQVMAGFDPRDSTSIDQPVPDYLGQLDQPLKGLRIGLPKEYFEAGLSAPMERV
                     IRAAIEIYRDLGAQFREVSLPHTALSVPVYYVIAPAECSANLARYDGVRYGHRCADPV
                     DLEDLYTRSRAEGFGAEVQRRILTGTYVLSSGYYDAYYLKAQKIRRLIRDDFARAFDE
                     VDLLLGPTSPSPAFKLGEKSSDPITMYLEDIYTIAANLAGLPALSIPAGLLDGLPVGL
                     QLIGNYFDEGRLLAAAHRFQQQTGWHQREPLSSSTLQAS"
     gene            2506..>3593
                     /gene="gatB"
                     /locus_tag="PKI23_11180"
     CDS             2506..>3593
                     /gene="gatB"
                     /locus_tag="PKI23_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNN37537.1"
                     /translation="MEWETVIGLEIHVQLATQSKIFSGASTRFGARPNGQACAVDLAL
                     PGVLPVLNEAAVRLAVRFGLAIGAQIDRQSIFARKNYFYPDLPKGYQISQMEHPIVGR
                     GQVEITLADGSTRTVGITRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPDL
                     RSAEEAVAYLRTIHTLVRWLKVSDGNMAEGSLRCDANVSVRPRGQRELGTRTEIKNIN
                     SFRFVERAIRHEVERQIELIEAGGRVVQETRLYDSVRDETRSMRSKEVATDYRYFPEP
                     DLLPLQLSEAFIEAVRGELPELPEARCARFISAHQLTPYDAGVLTSSRELADYYEAVA
                     AVVGDCKLAANWVTGELMGRLNRENLDIT"
BASE COUNT          630 a         1159 c         1148 g          656 t
ORIGIN      
        1 cgagccgctg gcctcctcga ccggcatgcc ggcaccgatg gcggcggcca tcggctcctc
       61 gatcaggcgc acgtcacggg cgcctgcgcc tatcgccgat tcgcggatcg cgcggcgttc
      121 gacctgggtc gaaccacagg gaatgctgat cagcacccgt gggctggggc gaatgaagct
      181 gttggcatgg gtcttggcga tgaagtgctg caacatcttc tcggtcacct gaaagtcggc
      241 gatgacgcca tctttcagcg gccggatcgc ggtgatgttg ccgggggtgc gcccgagcat
      301 ccgtttggct gcgctgccga cggcgcagac ggtcttctgg ccattttgaa tgcggatcgc
      361 caccaccgag ggctcatcca gcacgatgcc cttgccgcgc acatagatca gggtgttggc
      421 agtgccaaga tcgatcgaaa gatcgttgga gaacattcca cgcagtcgtt tgaacatatt
      481 cagctggcaa acctcgggat gggtgggcgc tcatgcggtg cacagcgaaa gcggtcgggt
      541 tcgaagggtg actgcaaacg cattctcgac cggcttccga cagtgcgggt cattgggggc
      601 agggtaaaaa tgtgataatt ttaccgcagt tgccccctgt ggggcagggg ctgagcaaag
      661 agttgcatca tcggtacaga gcagcacgga ttgaagatga aaccagaaga gattcagcgc
      721 atcgcccagc tcgcccggct caggatcgat gtcgatcacc atgcggaggt cggcgtgcgg
      781 ttgtcggcga ttctcgatct ggtgcagcag atgcatcaga tcgacacgca gaacatcgag
      841 ccgctggccc accccctcaa cgtcacccag cggctgcgtg gcgatgaggt caccgaaacc
      901 gaccagcgcg agcgttttca gcagctcgcg cccttgaccg aggatgggct ctatctggta
      961 cccaaggtga tcgagtgagc cgtctgaact gctgccgtcc gcgttccatc accccaccag
     1021 cacaccctgc atgcataatc tgactctgac ccaactggcg catgggctcg atgcgggcga
     1081 gttcagctcg gtcgagctga cacgccactt cctgcagcgc atcgacagcc tcgacggcca
     1141 ctacaacagc ttcatcacgc gaaccgccga gcaggcgctg gatgcagcgg ccgcggcgga
     1201 tgcgcgtcgc gcgcgtggcg aagctggcct gggtgccggc ctgccgttgg cgcacaagga
     1261 catcttctgc accgatgggg tgcgcaccac ctgcggttcg cggatgttgg gcaatttcgt
     1321 ctcgccctac gatgccaccg tggtcgagcg gtttcgtgcc gccggctggg tctgtctcgg
     1381 caagaccaac atggatgagt tcgcgatggg ctcctccaac gagaccagct attttggtcc
     1441 ggtgcgcaac ccctgggatc ggtcgcgggt gccgggcggt tcatccggtg gttcggccgc
     1501 ggcggtggcg gcgcggctgg cgcccggcgc cactgccagc gacaccggcg gctcgattcg
     1561 ccagccggcc gcgctctgcg gcatcagcgg cctgaagccg acctatggcc gggtctcgcg
     1621 ctacggcatg atcgccttcg cctccagcct cgatcagggt ggaccgatgg cgcgctcggc
     1681 ggctgacctt tcgctgatgt tgcaggtgat ggccggcttc gatccgcgtg attcgaccag
     1741 catcgaccag ccggtgcccg actacctggg tcagctcgac cagccgctga aggggctgcg
     1801 catcggttta ccgaaagagt atttcgaagc gggactgagc gcaccgatgg agcgggtgat
     1861 ccgcgctgcg atcgagatct atcgcgacct cggcgcgcag ttccgcgagg tgagtctgcc
     1921 ccacaccgcg ctgtcggtgc cggtctacta tgtcatcgcc ccggcggagt gctccgccaa
     1981 cctggcgcgc tacgatggtg tccgctatgg ccaccgctgc gccgatccgg tcgacctgga
     2041 agatctctac acccgtagcc gtgccgaggg ctttggcgcc gaagtgcagc ggcgcatcct
     2101 caccggcacc tacgtgctct cctccggcta ctatgacgct tactacctga aagcgcagaa
     2161 gatccgccgg ttgatccgtg acgacttcgc ccgtgctttc gacgaggtcg atctgctgct
     2221 gggccccact tcgccctcac cggccttcaa actcggcgag aagagcagcg acccgatcac
     2281 catgtatctg gaggacatct acaccatcgc cgccaacctg gccgggttgc cggcgttgtc
     2341 gatcccggcc ggtctgctgg atggcctgcc ggtcggtctg caactgattg gcaactactt
     2401 cgacgagggg cgactgctgg cggcggcaca ccgcttccag caacagaccg gctggcacca
     2461 gcgtgaaccc ctgtcctctt caaccttgca ggcatcatga ccgctatgga atgggaaacc
     2521 gtcatcgggc tggagatcca tgtccagctc gcgactcaga gcaagatctt ctccggtgcc
     2581 agcacccgct tcggcgcccg cccgaatggc caggcctgcg cggtcgatct ggcactgccg
     2641 ggggtgttgc cggtgctgaa cgaggctgcg gtgcggctgg cggtgcgctt tggtcttgcc
     2701 atcggtgccc agatcgatcg ccagtcgatc ttcgcccgca agaactactt ctaccccgat
     2761 ctgccgaagg gttatcagat cagccagatg gagcacccga tcgtcggacg tggtcaggtg
     2821 gagatcaccc tggccgatgg ttcgacccgc acggtcggca tcacccgcgc ccacctcgaa
     2881 gaggatgccg gcaaatcgct gcacgaagat tttcatggca tgaccggcat cgacctcaac
     2941 cgcgccggca cgccgctgct cgaaatcgtc tccgagcccg acctgcgttc ggccgaggag
     3001 gccgttgcct acctcagaac catccacacc ctggtgcgct ggctgaaggt ctccgacggc
     3061 aacatggccg aagggtcgct gcgctgcgat gccaatgttt cggtgcgccc gcgtggccag
     3121 cgtgaactcg gcacccgcac cgagatcaag aacatcaact cgttccgctt cgtcgagcgg
     3181 gcgattcgcc atgaggtcga gcggcagatc gagctgatcg aagccggcgg acgggtggtg
     3241 caggagaccc ggctctacga ttcggtgcgt gatgagaccc ggtcgatgcg cagcaaggag
     3301 gttgccaccg actaccgcta ctttcccgag cccgatctgt tgccgttgca gctgagcgaa
     3361 gccttcatcg aggcggtacg cggcgaactg cccgaactgc ccgaagcgcg gtgcgcccgg
     3421 ttcatcagcg cacatcaatt gacgccctac gacgcggggg tgctgacctc ctccagagag
     3481 ctggccgatt actacgaggc ggtggcagcg gtcgtcggcg attgcaagct ggccgccaac
     3541 tgggtcaccg gcgagttgat ggggcgactc aaccgcgaga acctcgacat cac
//
LOCUS       DAORCQ010000154         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_21515_length_3573_cov_4.592098, whole genome shotgun sequence.
ACCESSION   DAORCQ010000154 DAORCQ010000000
VERSION     DAORCQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21515_length_3573_cov_4.592098"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKI23_11185"
     CDS             <1..476
                     /locus_tag="PKI23_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNN37538.1"
                     /translation="VDSYGGMARHGGGAFSGKDPTKVDRSAAYACRYVAKNIVAAGLA
                     ERCEIQVSYAIGVAEPTSIAVDTFGTGKVSEARIVQLIREHFDLRPYAIGRMLDLGHP
                     MYQATAAYGHFGRNPVEMRYDWSERENGGEGQKSETFTAFTWERTDKAEALRAAI"
     gene            508..1902
                     /gene="ahcY"
                     /locus_tag="PKI23_11190"
     CDS             508..1902
                     /gene="ahcY"
                     /locus_tag="PKI23_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNN37539.1"
                     /translation="MSLANPVSKSTADYRVADINLADFGRREIALAEREMPALMGLRA
                     RYRGERPLTGARIVGCIHMTIQTAVLIETLIELGGEVRWSSCNIFSTQDHAAAAIAAS
                     GVPVFAWKGETEEEYWWCIEQTLHVDGKLWNPNMILDDGGDLTALIHQNYPQLLGQIH
                     GVTEETTTGVHRLEQMLKRGELKVPAINVNDSVTKSKNDNKYGCRHSLTDAIKRGVDH
                     LLAGKRALVIGYGDVGKGSAASLRQEGMIVRVVEIDPICAMQACMDGFELVSPYRNGL
                     NTGRPEDLNHALLAETELLVTATGNVRVCDRAMLAALKSGAVVCNIGHFDNEIDTQFM
                     RDNWEWVEVKPQVHQIYRDRASDDFLLLLSEGRLVNLGNATGHPSRIMDGSFANQVLA
                     QMHLYGQRFAEQSPARQQTLLKIEVLPKKLDEEVAAAMVAGIGGVLTRLTAEQADYIQ
                     VPVDGPYKPEQYKY"
     gene            1919..2779
                     /gene="metF"
                     /locus_tag="PKI23_11195"
     CDS             1919..2779
                     /gene="metF"
                     /locus_tag="PKI23_11195"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNN37540.1"
                     /translation="MVVTSSGFQLSFEFFPPKSEPGLEKLRSTWTQLDQFAPTFFSVT
                     YGAGGSTRDGTLNTVLAMRAAGLDVAPHLSFGGDSREQIGELLDRYRQAGIGRIVALR
                     GDLPSGLGSRRLAYASELVEFIRGHSGDHFTIEVAAYPEVHPESASYASELHHFAHKV
                     AAGANAAITQYFYNVDAYCHFVEACRRAGLDLPIVPGIMPITNYERLLRFSDSCGAEV
                     PRWIRKKLEGHRDDEAALLAFGADVVTRLCEQLLAAGAPGLHFYSLNQAEPTATLCRR
                     LGLAEHAAPA"
     gene            2776..>3573
                     /locus_tag="PKI23_11200"
     CDS             2776..>3573
                     /locus_tag="PKI23_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNN37541.1"
                     /translation="MNHPSEGDCLNAGDNRHWMARDLQVVWHPCTQMKDHESQPLIPI
                     RHGQGVWLEDFDGKRYLDAVSSWWVNLFGHGHPHINGALRDQLERIQHVMLAGFTHPP
                     VVELSEQLVRVTPPGLDRCFYADNGSSAVEIALKMSFHYWRNVGHSRKQRFVTLGNSY
                     HGETLGALAVGDVALYKETYAPLLLEAITVPSPDCFNREPGESWEAYSRRSFAAMETT
                     LARHADEVCAVIVEPLIQCAGNMRMYHPVYLSLLREACDRHGVHLIAD"
BASE COUNT          661 a         1150 c         1134 g          628 t
ORIGIN      
        1 tcgtcgacag ctatggcggc atggcccgcc atggcggcgg cgccttctcg ggcaaagatc
       61 ccaccaaggt cgatcgctcg gcggcctatg cctgccgcta tgtcgccaag aacatcgtgg
      121 cggccggcct ggccgagcgc tgcgagatcc aggtcagcta tgccatcggc gtggccgaac
      181 cgacctcgat cgcggtcgac accttcggca ccggcaaggt gagtgaggcg cgcatcgtcc
      241 agttgatccg cgagcatttc gatctgcgcc cctatgccat cggcagaatg ctcgatctgg
      301 gccacccgat gtatcaggca actgccgcct acggccactt tggccgcaat ccggtcgaga
      361 tgcgttacga ctggagcgag cgtgagaatg gtggtgaagg gcagaaatcc gagactttca
      421 ccgccttcac ctgggagcgc accgacaagg ccgaagcgct gcgcgccgcg atctgagccc
      481 gacccaacct gttcaacgga gtgttcgatg agccttgcca accctgtttc aaagtcgact
      541 gccgactacc gcgtcgccga catcaatctg gctgatttcg gccgccggga gatcgcactg
      601 gccgagcgcg agatgccggc actgatgggg ctgcgtgccc gctaccgcgg cgagcggcca
      661 ctgaccggcg cccggatcgt cggctgcatc cacatgacga tccagactgc cgtactgatc
      721 gagaccctga tcgagttggg gggcgaggtg cgctggtcct cctgcaacat cttctcgacc
      781 caggaccacg cggccgccgc catcgccgcc agcggcgtgc cggtctttgc ctggaagggc
      841 gagaccgaag aggagtactg gtggtgcatc gagcagactc tgcatgtcga tggcaagctg
      901 tggaacccca acatgattct ggatgatggc ggtgacctca ccgcactgat ccatcagaac
      961 tatccgcaac tgctgggtca gatccacggc gtcaccgagg agaccaccac cggcgtacac
     1021 cggctggagc agatgctcaa acgtggtgaa ctcaaggtgc cggcgatcaa tgtcaacgac
     1081 tcggtcacca agtcgaagaa cgacaacaag tatggctgcc gtcacagcct gaccgatgca
     1141 atcaagcgtg gcgtcgatca cctgctggcc ggcaagcggg cgctggtgat cggctatggt
     1201 gacgtcggca agggctccgc ggcctcgctg cggcaggagg gaatgatcgt ccgcgtcgtc
     1261 gagatcgatc cgatctgcgc gatgcaggcc tgcatggatg gtttcgagct ggtctcgccc
     1321 tatcggaacg ggctcaacac cgggcggccg gaggatctga accacgcgct gctggccgaa
     1381 accgagctgc tggtgaccgc caccggcaat gtccgggtct gtgaccgggc gatgctggct
     1441 gcgctgaaga gcggagcggt ggtctgcaac atcggccact tcgacaacga gatcgacacc
     1501 caattcatgc gcgacaactg ggagtgggtc gaggtcaagc cgcaggtgca ccagatctac
     1561 cgcgaccgcg ccagcgatga ctttctgctg ctgctctccg aagggcggct ggtcaacctc
     1621 ggcaatgcca ccggccatcc gtcgcgaatc atggatggct ccttcgccaa ccaggtgctg
     1681 gcgcagatgc atctgtatgg ccagcgtttt gccgagcaga gcccggcccg tcagcagacg
     1741 ctgctgaaaa tcgaggtgct gccgaagaag ctcgacgaag aggtggcggc agcgatggtg
     1801 gccggcattg gcggtgtgct gacccggctg accgccgagc aggccgacta catccaggtg
     1861 ccggtggacg ggccctacaa gcccgaacag tacaaatact gagcagggaa cctcagccat
     1921 ggtcgtgact tcgtctgggt ttcagctcag cttcgaattc tttccgccga agagtgaacc
     1981 gggcctggag aagttgcgca gcacctggac gcagcttgac caatttgcgc caacgttctt
     2041 ttcggtcacc tatggggccg gaggttcgac ccgcgatggc acgctgaaca ccgtgctggc
     2101 gatgcgggcc gccggactcg atgtcgcacc gcatctttca ttcggtggcg acagccgcga
     2161 gcagatcggt gaactgctcg accgttaccg gcaggcaggc atcggccgca tcgtcgccct
     2221 gcgtggcgac ctgccttcgg ggctgggcag ccggcggctt gcctatgcca gcgaactggt
     2281 ggaattcatc cgcggccaca gtggcgacca cttcaccatt gaggtggccg cctatcccga
     2341 ggtgcacccc gagtcggcca gttatgccag tgagctgcac catttcgccc acaaggtggc
     2401 ggccggtgcc aacgctgcga tcacccagta cttctacaac gtcgatgcct actgccactt
     2461 tgtcgaggcg tgccggcgcg cagggctcga tctgccgatc gtgccgggca tcatgccgat
     2521 caccaattac gagcggctgc tgcgcttctc cgacagttgt ggtgccgaag tgccgcgctg
     2581 gatcaggaag aagcttgaag gccatcgtga cgacgaagca gcactgttgg cctttggcgc
     2641 cgacgtggtg acccgcctgt gtgaacagct gctggccgcc ggggcaccgg gactgcactt
     2701 ctacagcctc aatcaggccg agccgaccgc caccctctgc cgccgtcttg ggctggccga
     2761 acacgccgct ccggcatgaa tcatccgtcc gagggcgact gcctgaacgc tggtgacaac
     2821 cgccactgga tggcgcgcga cctgcaggtg gtgtggcacc cctgcacgca gatgaaggac
     2881 cacgaatcgc aaccgctgat cccgatcaga catggccagg gggtctggct cgaggatttc
     2941 gacggcaagc gctatctcga tgccgtcagc tcctggtggg tcaacctgtt cggccatggc
     3001 catccccaca tcaacggcgc cctccgcgat cagctggagc ggattcagca tgtgatgctg
     3061 gccggcttca cccatccacc ggtggtggaa ctttcagagc agctggtgcg ggtcacgcca
     3121 cccgggctcg accgctgctt ctatgccgac aatggctcct cggcggtcga gatcgcgctg
     3181 aagatgagct ttcactactg gcgcaacgtc gggcacagcc gcaaacagcg cttcgtcacc
     3241 ctcggtaaca gctaccatgg cgagaccctc ggcgcgctgg cggtgggcga tgtcgcgctc
     3301 tacaaggaga cctacgcacc gctgctgctc gaagcgatca ccgtgccgag ccccgactgc
     3361 ttcaaccgcg agccgggtga gtcgtgggaa gcctacagcc gccgcagctt cgcggcgatg
     3421 gagaccaccc tggcgcgtca cgccgatgag gtgtgcgcgg tgatcgtcga acccttgatc
     3481 cagtgcgccg gcaacatgcg catgtaccac ccggtctacc tgagcctgct gcgcgaagcc
     3541 tgtgatcgcc atggtgtcca tctgatcgcc gat
//
LOCUS       DAORCQ010000155         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_23351_length_3398_cov_5.321268, whole genome shotgun sequence.
ACCESSION   DAORCQ010000155 DAORCQ010000000
VERSION     DAORCQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23351_length_3398_cov_5.321268"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKI23_11205"
     CDS             complement(<1..344)
                     /locus_tag="PKI23_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNN37542.1"
                     /translation="MSAFEIPLSDPDLTQLELDAVIDLLKSTRISSGPVVEAFEAAFA
                     TYLGRRHAIAVASGTAGLWLLLRACEIGPGDEVVASAFGWQPLAHAIALSGARPLFAD
                     VDYWSGTMSPEKA"
     gene            complement(341..625)
                     /locus_tag="PKI23_11210"
     CDS             complement(341..625)
                     /locus_tag="PKI23_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37543.1"
                     /translation="MREAVLTMELLAELEGWLSSPDLTRQQLPALRQSLAGIGLHSCD
                     AADVDEAPFRDYPNCALHLLDTRNHCVQITRDPARATALLLAMRRPGAQR"
     gene            complement(618..830)
                     /gene="nifT"
                     /locus_tag="PKI23_11215"
     CDS             complement(618..830)
                     /gene="nifT"
                     /locus_tag="PKI23_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HNN37544.1"
                     /translation="MKVMIRKSSSGQLSAYVPKKDLEEPIVAMEKPDLWGGTVTLANG
                     WRLLLPALAAETTLPLTVDARRMDDA"
     gene            complement(907..1329)
                     /locus_tag="PKI23_11220"
     CDS             complement(907..1329)
                     /locus_tag="PKI23_11220"
                     /inference="COORDINATES: protein motif:HMM:NF022671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HNN37545.1"
                     /translation="MERQERESAATPSLQTTLLHLPGRPAEAVVQQGSAVHHPNEFDR
                     HRIGAMLVHRRRYRYVQPTVVPESSGYRIESPCCSRNLEPDGGVIDIARLEFVIGRCW
                     RLFRKEHQSGQWQLHGVYASLRAALAPVNADPKRLFWP"
     gene            complement(1362..1604)
                     /locus_tag="PKI23_11225"
     CDS             complement(1362..1604)
                     /locus_tag="PKI23_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNN37546.1"
                     /translation="MIDRRPPRYDCGQQVRAQSDLYNDGSHPLAEAGALLIEAGAVGE
                     IVRVGLHQQANVPVYLVDFIEHRAVLGCLEEELVPL"
     gene            complement(1601..1921)
                     /locus_tag="PKI23_11230"
     CDS             complement(1601..1921)
                     /locus_tag="PKI23_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNN37547.1"
                     /translation="MGDISRDSDSTELHGPPAFAYGDRVRSRRMVRNDGTFPGREIGD
                     VLVRKGDIGYVTSIGTFLQRFYIYGVDFIDHGYCVGMKGRELEPLPWSEQVDATPSSS
                     GSTP"
     gene            complement(1927..2706)
                     /locus_tag="PKI23_11235"
     CDS             complement(1927..2706)
                     /locus_tag="PKI23_11235"
                     /inference="COORDINATES: protein motif:HMM:NF017312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HNN37548.1"
                     /translation="MTSEIATLTWIGQPVDCRQCDHHALQRDGGCSPGHACMNDRYAP
                     RIDRFFARHPQLAGSHLDHPYFEVRAVAARHVDLFQLRRLVDDPDEIVRWSAAQRLPL
                     RQLLPLIHDPSREVRIRVAGRLPPEALRAMADDADYYVRQIVARRLPAGLLRVMLDDP
                     DPGVRRLIALRADEALLPQLWNDPDPEVRLLLVERAAPRQLLHWLDDPDWRVRHAVAR
                     RIEPGHLPQLADDPDPIVRELVRQRRCGGDGLASNDLASME"
     gene            complement(2696..3256)
                     /locus_tag="PKI23_11240"
     CDS             complement(2696..3256)
                     /locus_tag="PKI23_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37549.1"
                     /translation="MSAASHWITDLHCASRPAALLADADWAAACLGGALPEAVEAALR
                     AAGSAYADDDLAERHLLQASALAPDHPAVLIAWYRFCFYKGRLMQARELGLRCLTLAA
                     RGCGLPESWQTVSATAARFDDFAAVAERFYLFSLKGCAYLSLRLGDLSLGQAMLAKLR
                     ELDPADRLGGAVLEQVLARRGADDDE"
BASE COUNT          582 a         1103 c         1158 g          555 t
ORIGIN      
        1 gccttctcgg gcgacatggt tcccgaccag tagtcgacat cggcaaagag cgggcgggcg
       61 ccgctcaggg cgatggcatg ggccagcggc tgccagccga acgccgaggc gaccacttcg
      121 tcacccgggc cgatttcgca ggctcgcagc agcagccaca gacccgcggt gccgctggcc
      181 accgcgatgg cgtggcgccg tcccagatag gtggcgaagg cggcttcgaa agcctcaaca
      241 accggtccgg atgagatgcg cgtcgatttg agcagatcga tcacggcatc gagttcgagc
      301 tgcgtcagat cgggatcgga gagcggaatc tcgaatgcgc tcatcgttgg gcaccgggtc
      361 ggcgcatggc cagcagcagt gcggtggcac gggcgggatc gcgggtgatc tgcacgcagt
      421 ggttgcgggt gtcgagcaga tgcagcgcgc agttggggta gtcgcgaaag ggtgcctcgt
      481 cgacatcggc cgcatcgcag ctgtgcagac cgatgcccgc cagcgattga cgcaatgccg
      541 gcaactgctg gcgggtcaga tcgggcgacg acagccagcc ttccagttcg gccagcagtt
      601 ccatggtcag caccgcttca cgcatcgtcc atgcgcctgg catcgacggt gagtggcagg
      661 gtcgtctccg ccgccagggc cggcagcagc agtcgccagc cattggcgag cgtgacggtg
      721 ccgccccaca ggtcgggttt ctccatggcc acgatcggct cttccagatc ctttttgggc
      781 acataggccg acaactggcc cgaggaactc ttgcgaatca tcactttcat cggtttttcc
      841 gtggtttgcc cgggtggagg tgggggtgcg acagcagcgc cgccgtggag tgaatcgatc
      901 gaccactcat ggccagaaca gccgcttcgg atcggcattg accggtgcca gcgcggcgcg
      961 caggctggcg tagacgccat ggagctgcca ctgaccgctc tggtgctcct tgcggaacag
     1021 ccgccagcag cgaccgatga cgaactccag acgggcgatg tcgatcacgc ctccgtccgg
     1081 ctccaggttg cgtgaacagc aggggctctc gatgcggtag ccgctgctct ctggtacgac
     1141 cgtcggttgc acatagcggt agcggcggcg gtgaaccagc atcgcgccga tgcgatggcg
     1201 gtcgaactcg ttggggtgat gtacagcgct gccctgctga accactgctt cagcgggccg
     1261 ccccggcaga tgcagcagcg tggtttgcag tgatggcgtt gctgccgact cacgctcttg
     1321 gcgctccacg actcacctcg tccggttgca gttcgatggc ttcacagcgg taccagctcc
     1381 tcttccaggc agcccagcac cgctctgtgc tcgatgaaat cgaccaggta gaccggaaca
     1441 ttggcctgct gatgcaggcc gacccgcacg atctcgccga ccgccccggc ctcgatcagc
     1501 agggcgccgg cctcggccag tggatgggag ccgtcgttgt agaggtcgct ttgggcgcgg
     1561 acctgctggc cgcagtcgta gcgcggcggt cgccgatcga tcatggcgtg ctcccggatg
     1621 aggatggggt ggcatcgacc tgttccgacc agggcagggg ctccagttcg cggcccttca
     1681 tgccgacgca gtagccatgg tcgatgaaat cgacgccgta gatgtagaaa cgctgcaaaa
     1741 aggtgccgat gctggtgaca tagccgatgt cgcccttgcg caccagcaca tcgccgattt
     1801 cacgtccagg aaaggtcccg tcgttgcgca ccatgcgcct cgatcgcacc cggtcgccat
     1861 aggcgaaggc cggcgggcca tgcagttcgg tcgaatcgct gtcacggctg atgtcgccca
     1921 tcattgctac tccatcgaag ccagatcgtt cgaagccagg ccatcgccac cgcaacggcg
     1981 ttgccgcacc agttcacgca cgatggggtc cggatcgtcc gccagttgcg gcagatggcc
     2041 gggttcgatc cgtctggcca ccgcgtggcg gacccgccag tcaggatcat cgagccagtg
     2101 gagaagctgc cgtggtgcag cgcgctccac cagcagcagc cgcacttccg ggtcgggatc
     2161 gttccagagc tgcgggagca gcgcctcatc ggcccgcagc gcgatcagcc tgcgcacgcc
     2221 ggggtcggga tcgtccagca tcactctgag caagccggcc ggcaggcggc gcgcgacgat
     2281 ctgacgcacg tagtaatcgg catcgtcggc catcgcccgc aatgcctcgg gtggcagccg
     2341 tcccgccacc cggatccgca cctcgcggct cgggtcatgg atcagcggca acaactggcg
     2401 cagcggcagg cgctgtgccg cactccagcg cacgatctca tccgggtcat cgaccagacg
     2461 gcgcaactgg aacaggtcga catgacgcgc cgcgaccgca cgcacctcga aataggggtg
     2521 gtcgagatgg ctgccggcga gttgggggtg acgggcaaag aagcggtcga tgcgtggcgc
     2581 atagcggtca ttcatgcagg catggccggg gctgcagccg ccatcgcgtt gcagtgcatg
     2641 gtggtcgcac tggcggcaat cgaccggttg cccaatccag gtgagcgtgg cgatctcact
     2701 cgtcatcatc cgctccccgg cgtgccagca cctgttccag caccgcgccg cccaggcggt
     2761 cggccggatc gagttcgcgc aacttggcca gcatggcctg accgaggctc agatcgccca
     2821 gccgcaggct cagataggcg cagcccttca gggagaagag atagaagcgc tcggccactg
     2881 cggcaaagtc atcgaagcgt gccgcagtgg cgctcaccgt ctgccagctc tctggcagac
     2941 cacagccgcg ggcagcgagc gtaagacagc gcagccccag ttcgcgtgcc tgcatgagcc
     3001 ggcccttgta gaaacagaac cggtaccagg cgatcagcac ggccggatgg tcgggtgcca
     3061 gggccgaggc ctgcagcagg tgccgttcgg ccagatcgtc atcggcatag gccgagccgg
     3121 cggcgcgcag tgctgcttcg actgcctcgg gcagcgcccc gccgaggcag gccgcagccc
     3181 agtctgcatc ggccagcagg gcagcgggcc gcgacgcgca gtgcaggtcg gtgatccagt
     3241 ggctggcggc tgacattggc tttcactgcc cattcggttg aacgcggcgg acgatggcgg
     3301 agaccgccac cgcgggagtg gccgtcgtgc tgctgcaatt gccggctccg gcgacgttgc
     3361 ggaaggtcag gcccgactcg gtggccgttt cggcaaag
//
LOCUS       DAORCQ010000156         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_23836_length_3357_cov_6.649909, whole genome shotgun sequence.
ACCESSION   DAORCQ010000156 DAORCQ010000000
VERSION     DAORCQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23836_length_3357_cov_6.649909"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1229
                     /gene="pgi"
                     /locus_tag="PKI23_11245"
     CDS             60..1229
                     /gene="pgi"
                     /locus_tag="PKI23_11245"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNN37550.1"
                     /translation="MATEALRPYHTGRFRSHFLANIDGADFERTLHGLDPATTLFIVA
                     SKSFGTLETRKNAEAARAWVLADSGDPAAVARHFVAVSANVAKAVAFGIDERQVFPLW
                     DWVGGRYSLWSAIGLPIMFAIGVNGFRALQQGAQAMDSHFAQAELAHNMPVLLALLGI
                     WYQNLWGARSQAILPYLHDLRRFPDFLQQLEMESNGKSCNLAGEPLAYHSAAVVWGTP
                     GTLGQHSFHQLLHQGTQFIPVDFITALRAHHRVADHHHHLFANCVAQSQALLEGKSLE
                     AATAELLQAGFDHEQARQLAPHKVIAGNRPSNMLLMEQLTPQTLGALIALYEHKVYVQ
                     SLIWRINAFDQWGVELGKQLSERISPLLIDGTLPLPDQFDGSTAALIRRYRSGLL"
     gene            complement(1248..2555)
                     /locus_tag="PKI23_11250"
     CDS             complement(1248..2555)
                     /locus_tag="PKI23_11250"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNN37551.1"
                     /translation="MSDRSRAVPAPALLIRGGRVIDPVQGIDRVADLAVSDGRILSIG
                     EPPPGFVAEQQIDAQGWVVAPGFVDLAVRPREPGDEQKATLESECRAALAGGITTLCM
                     PPDTRPPIDTPAVVRLIEAQARAAGAARVVVLGALTQQLAGAFPAEMAALRAAGCVGM
                     SNGKAPLATARVLRHCMEYAASNGLMLFVEPEEPTLAADGCAHEGEVASRLGLTGIPA
                     SAESIAIATVLLLVEQTGARVHFGRLSAARSVALIAQAKAQGLPVTADVAAHQLIWNE
                     RALLDYDARFHLRPPLRGEADRQALLQGVLEGVVDAICSDHQPHEAEAKLAPFAATEP
                     GISALETLLPLALAAASQVEMPLIKLIERLSSAPARLLGLDAGSLLPGARADLCLFAP
                     AERWRLSAATLHSRGQNSPLIESELVGRVRHTLLDGRIVHQAS"
     gene            complement(2548..>3357)
                     /locus_tag="PKI23_11255"
     CDS             complement(2548..>3357)
                     /locus_tag="PKI23_11255"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNN37552.1"
                     /translation="FEASTRTRTTFELAAKRLSADVLSLNIGTSATSKGESLLDTLRN
                     LEAMASDMFIVRHADSGAAHFIAAQVTPDVAVINAGDGRHAHPTQAMLDMFTIRRHKG
                     AFEPLKVAIVGDILHSRVARSQIKALKTLGTGEVRLIAPLTLLPAGIDAFGVRHFTRL
                     EEGLSDVDVVIMLRLQRERMQSALLPSFNEYYQLYGLTTEKLAYARPDAIVMHPGPIN
                     RGVEIQSEVADGPQSVILQQVSNGIAVRMAILSMTMSGQTLARQQESALHV"
BASE COUNT          558 a         1178 c         1083 g          538 t
ORIGIN      
        1 accgaccgat caccgacatc gtcaacatcg gcatcggcgg ttcggacctc ggtccggtga
       61 tggccaccga ggcgctgcgc ccctaccaca ccggccgctt tcgcagccac tttctggcca
      121 acatcgatgg tgccgacttc gagcgcaccc tgcacgggct cgaccccgcc accacgctgt
      181 tcatcgtcgc ctccaagtcg ttcggcacac tcgaaacccg caagaatgcc gaggcagcac
      241 gcgcctgggt gctggccgat tcgggtgatc ccgccgccgt ggcgcgtcac ttcgtggcgg
      301 tgtcggccaa tgtcgccaag gcagtcgcct tcggcatcga cgaacggcag gtctttccgc
      361 tgtgggactg ggtcggtggc cgctactcgc tctggtcggc aatcgggctg ccgatcatgt
      421 tcgccatcgg cgtcaatggc tttcgcgcac tgcaacaggg cgcccaggcg atggacagcc
      481 actttgccca ggccgaactg gcacacaaca tgccggtgct gctggcgctg ctgggcatct
      541 ggtaccagaa cctgtggggc gcacgctcac aggcgatact gccctatctg catgacctgc
      601 gccgcttccc cgattttctg cagcaactgg agatggagag caacggcaaa tcctgcaacc
      661 tggccggcga accgctcgct taccacagcg ccgccgtggt ctggggcacg cccggcacgc
      721 tgggccagca ctcgtttcac caactgctgc atcagggcac tcagttcatc ccggtcgatt
      781 tcatcaccgc gctgcgcgcc caccaccggg tggccgacca ccaccaccat ctcttcgcca
      841 actgcgtggc acagagtcag gcgctgctgg agggcaagag cctcgaagcg gccaccgcag
      901 agctgctgca ggccggattc gaccacgaac aggcccgcca actggccccg cacaaggtga
      961 tcgccggcaa ccggcccagc aacatgctgc tgatggagca actgacgccg cagaccctgg
     1021 gcgcactgat cgcgctctat gagcacaagg tctatgtgca gagcctgatc tggcgcatca
     1081 atgcattcga ccagtgggga gtggagctgg gcaagcagtt gagcgaacgc atctcgccac
     1141 tgctgatcga cggcacgctg ccgctgcccg atcagttcga tggctcgacc gccgcactga
     1201 ttcggcgcta ccgcagcggc ctgctgtgaa agccgggagt gcgcccgtta cgacgcctgg
     1261 tggacgatcc gtccatcgag cagcgtatgg cgcacccgtc ccaccagttc gctctcgatc
     1321 agcgggctgt tctgccctct tgagtgcaac gtggcggcgc tcagtcgcca gcgctcagcg
     1381 ggggcaaaga ggcagaggtc ggcgcgggca cccggcagca gcgagccggc gtcgagtcca
     1441 agcaaccggg caggtgcgct gctgagccgt tcgatcagtt tgatcagtgg catctcgacc
     1501 tggcttgcag cggccagcgc cagtggcaac agcgtctcga gtgcggagat tcccggctcg
     1561 gtggcggcaa agggtgccag cttggcctcg gcctcgtgcg gctgatggtc ggagcagatg
     1621 gcatcgacca ccccctccag caccccctgc aacagcgcct ggcggtcggc ctcgccgcgc
     1681 agcggtggcc gcagatggaa gcgggcgtca tagtcgagca gcgcgcgctc gttccagatc
     1741 aactgatggg cggccacgtc ggcagtgacc ggcaatccct gcgccttggc ctgggcgatc
     1801 aacgccaccg accgggcggc cgacagccgt ccgaagtgga cccgcgcacc ggtctgctcg
     1861 accagcagca gcaccgtggc aatggcgatg ctctcggcac tggccgggat gccggtcagg
     1921 cccagacggc tggccacctc gccttcatgg gcacagccat cggccgccag cgtcggctcc
     1981 tcgggttcga caaacagcat caggccattg ctggccgcat actccatgca gtgtcgcagc
     2041 acccgcgcag tggccagcgg cgccttgccg ttgctcatgc caacgcaacc agcggcgcgc
     2101 agcgccgcca tctcggccgg aaatgccccg gccaactgct gcgtcagtgc gcccagcacc
     2161 accacccgtg ccgcaccggc cgcgcgcgcc tgcgcctcga tcagccggac caccgccggc
     2221 gtgtcgatcg gaggccgggt gtcgggtggc atgcagagcg tggtgatgcc gccggccagc
     2281 gcggcccggc attcactctc cagtgtcgcc ttctgctcat cgccgggctc gcgcggccgc
     2341 accgccagat cgacgaaacc cggcgccacc acccaaccct gggcgtcgat ctgctgctcg
     2401 gccacgaaac cgggcggtgg ctcgccaatc gacagaatcc ggccgtcgct gaccgccaga
     2461 tcggcgacgc ggtcgatgcc ctgaaccggg tcgatgaccc ggccaccgcg gatcagcagc
     2521 gccggtgccg gcacggccct ggatcgatca gacatggagc gcggactcct gctgtctggc
     2581 cagcgtctgg ccactcatcg tcatcgacag aatcgccatc cgcacggcga tgccgttgct
     2641 cacctgctgc aggatcaccg actgcggccc gtcggccacc tcggactgga tctcgacgcc
     2701 gcggttgatc ggtcccggat gcatgacgat cgcatcgggc cgggcatagg ccagtttttc
     2761 ggtggtcagc ccataaagct ggtaatactc attgaagctc ggcagcagcg cgctctgcat
     2821 ccgctcgcgc tgcaaacgca gcatgatcac cacatcgacg tcgctcagcc cctcttcgag
     2881 ccgggtgaag tggcgcactc cgaaggcgtc gatgccggcc ggcagcagcg tgagcggggc
     2941 aatcagccgc acctcgccgg tgcccagcgt cttcagtgcc ttgatctgcg agcgggcgac
     3001 gcgggagtgc aggatgtcgc cgacgatcgc caccttcagc ggctcgaagg cccccttgtg
     3061 gcggcgaatc gtgaacatgt cgagcatcgc ctgcgtcgga tgggcatggc gcccatcacc
     3121 ggcattgatc accgccacgt cgggcgtcac ctgggcggcg atgaagtggg ccgcgccgct
     3181 gtcggcatgg cgcacgatga acatgtcgct ggccatcgcc tccagattgc gcagggtgtc
     3241 gagcaggctc tcgcccttgg aggtggccga ggtgccgatg ttgaggctga gcacatcggc
     3301 cgacagccgc ttggcggcca gctcgaaggt ggtgcgggtg cgggtgctgg cctcgaa
//
LOCUS       DAORCQ010000157         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_25080_length_3264_cov_4.985042, whole genome shotgun sequence.
ACCESSION   DAORCQ010000157 DAORCQ010000000
VERSION     DAORCQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25080_length_3264_cov_4.985042"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..836)
                     /locus_tag="PKI23_11260"
     CDS             complement(69..836)
                     /locus_tag="PKI23_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNN37553.1"
                     /translation="MSEYYSLAVDGHVITVTLQQPEISNSLDSAACLALHELFDEFER
                     NPQWWVAVITGAGERAFCAGMNLKNVAKGEPLRLPPSGFAGLTHRFDNSKPVIAAVNG
                     AAYGGGLEMALACDIVIAAEHAIFALSEPRVGVAALAGGVHRLARQIGLKQAMGMLLN
                     GRKVSAAEAHELGFVNEVVPAAELMATAQRWAAEIAACAPLAVRATKQLAMSGLDLPL
                     REAMAASYPMVTAMLNSPDMVEGARAFAERRAPNWQG"
     gene            complement(884..1990)
                     /locus_tag="PKI23_11265"
     CDS             complement(884..1990)
                     /locus_tag="PKI23_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNN37554.1"
                     /translation="MDLSITTTRDARALQLRNWAAAILAENPGLSADPADGWRFAKLA
                     GDASFRRYFRLQRERQSWVLMDAPPAKEDVRPFIAVAGGLAQLGIRVPRVLAADLDQG
                     FLLLDDLGDRLYLGELTAADPQRVDQLYQAAFQPLLRLQSVSQLPGCKLPPYDRTLLH
                     SELRLFDQWFIAELLGVELTQSEQRMLHQLYDELIELALAQPQCPVHRDYHSRNLLLC
                     ADGMVGVIDFQDAVIGPITYDLVSLLKDCYLFWPPQQVSRWVTQYHRLAQNAALLSEV
                     SPEQFLHWFDWIGVQRHLKVLGIFSRLFLRDGKEGYLADLPRVARHLLAGCAPHGPLQ
                     PFAQWFRERLLPRMATHPRLDAVAVQAVVDEKIE"
     gene            2105..>3264
                     /gene="lptD"
                     /locus_tag="PKI23_11270"
     CDS             2105..>3264
                     /gene="lptD"
                     /locus_tag="PKI23_11270"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNN37555.1"
                     /translation="MKHPLPRSLFLFLVLLHTAPGQAESTHQSAAALDWHPREALPAE
                     MQARIPVTCPGLYIDPMANVELTAPANPEGAAADSVNIQASYVESLLAETFSLDGDVV
                     LEDSPWRMTAKRGNIDLPQGKWSLQEEVELRGKGLLLRGAKADYDRSGNTARMSDGQF
                     VLHERELHGNARQISVNRDSGIITLKQAALTGCPPGDDSWWLRSDYIRLDPKSGFGST
                     RNARIDLAGIPTLHVPYFYFPIDDRRHSGFLIPSLGNDLTNGLEATFPYYFNLAPNYD
                     YTLTPRLMSKRGTLLGNEFRYLTEQSRGDTRLNLLPDDLLGATSNPDRSQRWLYGIDH
                     RTFTPSGWDLSLDLNRASDRDYFVDLGGKGLDSHSLTNLRQAAEAQYRFDDLR"
BASE COUNT          665 a         1096 c          939 g          564 t
ORIGIN      
        1 gtcgcggcac ccgccagcaa aagagtactg aatcactgat ctttgcagtg tagcaattca
       61 aacagcactc agccttgcca gttgggtgcc cgcctttcgg caaaggcgcg cgccccttcg
      121 accatgtcgg ggctgttgag catggccgtc accatcgggt agctggccgc catcgcctcg
      181 cgcaggggca gatcaagacc cgacatcgcc aactgtttgg tcgcacgcac cgccaatggg
      241 gcgcaggccg caatctcggc ggcccaacgc tgtgcggtgg ccatcagttc cgcagccggc
      301 accacttcgt tgacgaaacc cagttcatgc gcttcggctg cgctcacctt gcgtccattg
      361 agcagcatgc ccatcgcctg cttgaggccg atctgccgcg ccagacgatg cacgccaccc
      421 gccagggcgg ccacgccgac ccgcggttcc gaaagcgcaa agatcgcatg ttcggcagcg
      481 atgacaatgt cgcaggccag cgccatctcc agaccaccgc cataggccgc gccattgacc
      541 gcggcgatca ccggcttgct gttgtcgaag cggtgggtga gtccggcaaa tccggacggc
      601 ggcagccgca gcggttcgcc cttggcgaca ttcttcagat tcatgccggc acagaaggca
      661 cgctctccgg caccggtgat gaccgcgacc caccactgcg ggttgcgctc gaactcatcg
      721 aacagctcat gcaaggccag gcaagccgca ctgtcgagcg agttggagat ctccggctgt
      781 tgcagggtca ccgtgatcac gtggccatcg accgccaggc tgtagtactc agacatgcaa
      841 tatcctccat gaataaatat ttttatcttt atgaaaaatc agatcattct attttctcgt
      901 caaccactgc ctgaaccgca acggcatcga gtcggggatg ggtggccatc cgcggcagca
      961 ggcgctcgcg aaaccactgt gcaaagggct gcaacgggcc atggggggcg cagcctgcca
     1021 acagatgtcg cgccacgcgc ggcagatcgg ccaggtagcc ctctttgccg tcgcgcagaa
     1081 acagccggct gaagatgccc agcaccttca ggtggcgctg cacgccgatc cagtcgaacc
     1141 agtgcagaaa ctgctccggc gaaacttccg acagcagggc cgcgttctga gccagccgat
     1201 ggtattgagt cacccagcga ctgacctgct gaggtggcca gaacagatag caatccttca
     1261 gcagcgacac cagatcgtag gtgatcggcc cgatcaccgc atcctgaaag tcgatcacgc
     1321 cgaccatgcc atcagcgcac agcagcaggt tgcgcgaatg gtagtcgcgg tgtaccggac
     1381 actgcggctg cgcaagcgcc aattcgatca attcgtcata aagctgatgc agcatccgtt
     1441 gctccgactg cgtcagctcg acccccagca actcggcgat gaaccactga tcgaacagcc
     1501 gcagttcgct gtgcagcagc gttcgatcat agggcggcag cttgcagcca ggcaactggg
     1561 acaccgattg cagccgcagc agcggttgaa aagcggcctg atagagctga tcgacccgct
     1621 gcggatcggc agcggtcaac tcacccagat agagtcgatc acccagatca tccagcaata
     1681 aaaaaccctg atcgaggtcg gccgccagca cccgtggcac ccggatgccg agctgtgcca
     1741 aaccacccgc cacggcgatg aagggacgca catcctcctt ggcgggggga gcatccatca
     1801 gcacccagct ctgccgttcc cgctgaagtc gaaaatagcg gcggaaactg gcatccccgg
     1861 ccagcttcgc gaagcgccag ccatcggccg gatcggcgct caaaccgggg ttctctgcta
     1921 gaatggccgc ggcccagttt cgcagttgca gggcgcgcgc atctcttgtc gtggtgatag
     1981 aaaggtccat gcccggtccg atgttcaaag tgctccatta tctgcccttc accctgctgc
     2041 caccagagca tgcaggagtc ggcgcgccat ctgcccgcct cggtcgtctg aagcggggtc
     2101 atggatgaaa catccgttgc cacggagcct cttcctgttt cttgtcctgc tgcacactgc
     2161 ccccggtcag gccgaatcga ctcaccagag cgcggcagcg ctcgactggc atccgcgcga
     2221 ggcgctgccg gccgagatgc aggccaggat acctgtgacc tgccccggcc tttacatcga
     2281 cccgatggcc aacgtcgaac tgaccgcacc ggccaacccg gagggcgcgg ccgccgattc
     2341 agtcaacatc caagccagtt atgtcgagtc gctgctggcc gaaaccttca gcctcgatgg
     2401 cgatgtggtg ctggaggatt cgccctggcg catgaccgcc aagcgcggca acattgattt
     2461 accgcagggc aaatggtcgc tgcaggagga ggtcgaactg cgcggcaagg ggctgctgct
     2521 gcgcggcgcc aaggccgact acgaccgctc cggcaacacc gccagaatga gtgacggtca
     2581 gttcgtgctg cacgagcgcg aactgcatgg caatgccaga cagatctccg tcaaccgtga
     2641 cagcggcatc atcacgctga aacaggctgc cctgaccggt tgcccacctg gcgacgacag
     2701 ttggtggctg cgcagcgatt acatccggct cgaccccaag agcgggttcg gcagcacccg
     2761 caacgctcgc atcgatctgg ccggcattcc gacgctgcac gtcccctatt tttacttccc
     2821 gatcgatgac cgtcgccaca gcggttttct gattcccagc cttggcaacg accttaccaa
     2881 cggcctggaa gcgaccttcc cttactactt caacctggcc cccaactacg actacaccct
     2941 gacgcccagg ttgatgagca agcgtggcac gctgctcggt aacgagtttc gctacctcac
     3001 cgaacagagc cgtggcgaca cccggctcaa cctgctgccg gacgacctgc ttggagcgac
     3061 atcgaacccg gaccgctcac agcgctggct ctatggcatc gaccaccgca ccttcacccc
     3121 ttccggctgg gatctctcgc tcgacctgaa ccgggccagc gaccgtgact actttgtcga
     3181 cctgggcgga aaagggctcg acagccactc gctgaccaac ctgcggcagg ctgccgaagc
     3241 gcagtaccgc ttcgatgacc tgcg
//
LOCUS       DAORCQ010000158         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_25205_length_3254_cov_5.798687, whole genome shotgun sequence.
ACCESSION   DAORCQ010000158 DAORCQ010000000
VERSION     DAORCQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25205_length_3254_cov_5.798687"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKI23_11275"
     CDS             complement(<1..751)
                     /locus_tag="PKI23_11275"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNN37556.1"
                     /translation="MELKERVCIITGAAGGIGQAAARRFHAEGARLMLVDLDPGPLGE
                     VARSIGPDVAWCQADVTQPEQVKRYVDETVARFGRIDGFLNNAGIEGVVAPITDSPIE
                     MFDRVMAVNVRGVWLGLKYVMAEMQKSGGGSIVITSSTAGIKGGPGLSPYVTSKHAVV
                     GMMRSASREGAPFKIRVNTVNPSPIDTRMMRSLEQGMAPSAPELAKERVAAGIPLGRY
                     GQPEEVAELMLFLVSDRASFLTGATYMVDGGI"
     gene            complement(774..1253)
                     /gene="rraA"
                     /locus_tag="PKI23_11280"
     CDS             complement(774..1253)
                     /gene="rraA"
                     /locus_tag="PKI23_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HNN37557.1"
                     /translation="MSISTPDLCDQFADRIRLVEPMFGNYGGRSAFGGQIVTIKCFED
                     NSLVKSQVGQNGRGKVLVVDGGGSSRRSLLGDMLAEEAAKNGWQGIVIYGAIRDVDAL
                     AETDLGVQALTTCPMKTEKRGIGDLDVPVTFGGVTFNPGEYLYADNNGIIVSASKLI"
     gene            1457..1978
                     /locus_tag="PKI23_11285"
     CDS             1457..1978
                     /locus_tag="PKI23_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNN37558.1"
                     /translation="MPQLEGSLFAQSLIFLCEHSAQGAMGLIVNRPLPMQLGEILDQL
                     QIDSHAGPAREAPLYFGGPVQSEQGFILHRGPARYLSSLQVSDEVVLCTSRDALEAIG
                     ANDGPADYLVALGYAGWSEGQLEQELADNAWLTVPATTDLLFTAEPSRRAAAAAATLG
                     INLSLLHAQPGHA"
     gene            1971..2465
                     /gene="ruvX"
                     /locus_tag="PKI23_11290"
     CDS             1971..2465
                     /gene="ruvX"
                     /locus_tag="PKI23_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNN37559.1"
                     /translation="MPEATPCSQQVVLGFDFGARRIGVAVGQGLTGRATPLRVLPARD
                     GIADWAQFDRLIAEWQPGSLLVGEPLNMDGSASEMTLRARKFANRLRERYRLPVHLVD
                     ERLSSFEARGRQLERRRQQRPPRSNEPGIDAQAACLIVETFLHEASMARRQGADINLE
                     QSNG"
     gene            2458..2967
                     /gene="pyrR"
                     /locus_tag="PKI23_11295"
     CDS             2458..2967
                     /gene="pyrR"
                     /locus_tag="PKI23_11295"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNN37560.1"
                     /translation="MVELPAVDLLLDAMAAALRQQLAALRISRPAMVGIRTGGVWVAE
                     QLHQRLGLDEPLGTLDIGFYRDDFTRIGLQPKVAPSQLPFAVDGRALLLVDDVIMSGR
                     TIRAALNELFDYGRPASVMLAVLIDLPQRELPIQPDVCGERLLLAPGERIKLTGPQPL
                     SLRLERTAP"
     gene            3008..>3254
                     /gene="pyrB"
                     /locus_tag="PKI23_11300"
     CDS             3008..>3254
                     /gene="pyrB"
                     /locus_tag="PKI23_11300"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551732.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNN37561.1"
                     /translation="MNSQPPHDLQLTPQGQLRHFLAIEGLSRQILTEILDTAESFLTV
                     GDQPVKKVPLLRGKTVVNLFFEASTRTRTTFELAAKRL"
BASE COUNT          504 a         1062 c         1097 g          591 t
ORIGIN      
        1 agataccacc atcgaccatg taggtcgcgc cggtcagaaa gctggcgcgg tcgctgacca
       61 gaaacagcat cagttcggcc acctcttccg gctggccgta gcggccaagc ggaatgccgg
      121 cggccacccg ctccttcgcc agttctggtg ccgacggcgc catgccctgt tccagtgaac
      181 gcatcatgcg ggtgtcgatc ggcgaggggt tgacggtgtt gaccctgatc ttgaacggcg
      241 ccccttcgcg cgatgcgctg cgcatcatgc cgacgacggc atgtttgctg gtgacatagg
      301 gcgacaggcc gggcccgccc ttgatgccgg cggtcgagga ggtgatgacg atgctgccac
      361 cgccgctctt ctgcatttcg gccatcacat acttgagccc gagccagacg ccacgcacat
      421 tgaccgccat cacccggtcg aacatctcga tcggtgagtc ggtgatcggc gccaccacgc
      481 cctcgatgcc ggcgttgttc agaaagccgt cgatgcggcc aaagcgtgcc acggtttcgt
      541 cgacatagcg cttcacctgt tcgggctggg tgacatcggc ctggcaccag gcgacgtcgg
      601 ggccgatcga tctggccact tcgccgagtg gccccgggtc gaggtccacc agcatcagcc
      661 gggcgccttc ggcgtggaag cgccgtgcgg ccgcctggcc gatgccgccg gcggcgccgg
      721 tgatgatgca gacacgctct ttgagctcca ttgttattct ccttcggtcg gtttcagatc
      781 agttttgaag cgctgacgat gatgccattg ttgtcggcat agagatactc gccggggttg
      841 aaggtgacgc cgccgaaggt gaccggcacg tcgaggtcgc cgatgccgcg cttttcggtc
      901 ttcatcggac aggtggtcaa tgcctgcacg ccgaggtcgg tctcggcgag cgcgtcgaca
      961 tcgcggatgg cgccatagat gacgatgccc tgccagccat tcttggcggc ctcctcggcc
     1021 agcatgtcgc cgagcagcga acggcggctg gaaccgccgc catcgaccac cagcaccttg
     1081 ccacggccgt tctggccgac ctggctcttg accagcgagt tgtcttcaaa gcatttgatg
     1141 gtgacgatct ggccaccgaa ggcggagcgg ccgccatagt tgccgaacat cggctcgacg
     1201 agacggatcc ggtcggcaaa ctggtcgcaa aggtcggggg tgctgatgct cattgctgct
     1261 cctgggttgt gcatggctgg gtgcgtgccg tctgccgttg cgcctgcaat cggcggcgct
     1321 gcgtgcgggt cggccgcgct ggccggcatg ttaccaccgc ggcggcttgt cgcatccggg
     1381 ctttcgcccg atactggcgc catgtcgaaa gagggagtcc ccggtgcaga cgttgcgcaa
     1441 tcaatttctg atcgcgatgc cacagctcga ggggtcgctc tttgcccaga gtctgatctt
     1501 tctctgcgag cacagcgcgc agggggcgat ggggctgatc gtcaaccggc cgctgccgat
     1561 gcagctcggc gagattctcg accagttgca gatcgacagc catgccgggc ccgcccgcga
     1621 ggcgccgctc tactttggcg gaccggtgca gagcgaacag ggcttcattc tgcaccgcgg
     1681 tcctgcgcgc tacctctcgt cactgcaggt gagtgacgag gtcgtgctgt gcacctcacg
     1741 cgatgcgctg gaggcgatcg gcgccaatga cggcccggcc gactatctgg tggcactggg
     1801 ttatgccggc tggagcgagg ggcagctcga gcaggagctg gccgacaacg cctggctcac
     1861 ggtgccggcc acgaccgatc tgcttttcac ggccgaaccg tcgcgccgcg ccgcggccgc
     1921 cgccgccacc ctcggcatca acctcagtct gctccatgcc cagcccggac atgcctgaag
     1981 cgacgccgtg cagccagcag gtcgtgctcg ggttcgactt cggcgccaga cgcattggcg
     2041 tggcggtggg gcaggggctc accggtcggg ccacgccact gcgggtgctg ccggcacgcg
     2101 acggcattgc cgactgggcg cagttcgacc ggctgatcgc cgagtggcag ccgggctcac
     2161 tgctggtggg cgagccactc aacatggatg gcagcgccag cgagatgacg ttgcgcgcgc
     2221 gcaagttcgc caaccggctg cgcgagcgct accggctgcc ggtgcatctg gtggatgagc
     2281 ggctctccag cttcgaggca cggggtcggc aactggaacg gcggcggcag cagcggccgc
     2341 cgcgatcgaa tgaacccggc atcgacgcgc aggccgcctg cctgatcgtc gagacctttc
     2401 tgcatgaagc gtcgatggcg cggcggcagg gcgcggacat caatctggag cagtcgaatg
     2461 gttgaacttc cggcggtgga tctgttgctc gatgcgatgg ccgcagcgtt gcgccagcag
     2521 ctcgctgcgt tgcgaatcag tcggccggcc atggtcggca ttcgcaccgg cggcgtctgg
     2581 gtggccgagc agctgcatca gcggctgggt ctcgacgaac cgctcggcac gctcgacatc
     2641 ggcttctacc gcgatgactt cacccgcatc ggcctgcaac cgaaggtggc gccgtcgcaa
     2701 ctgcccttcg cggtcgatgg tcgcgcgctg ctgctggtgg atgacgtgat catgagtggc
     2761 cggaccattc gcgccgcgct caatgagctg ttcgactatg gtcggccggc ctcggtgatg
     2821 ctggcggtgc tgatcgacct gccgcagcgt gagctgccga tccagcccga tgtgtgtggc
     2881 gagcggctgc tgctggcgcc gggtgagcgg atcaagctga ccgggccgca gccgctgtcg
     2941 ctgcgcctcg aacgcaccgc cccgtgattg ttggccgacc cggagccagc ggttacactg
     3001 cgcgccgatg aacagccagc cgccgcatga cctccagctc accccgcagg ggcagttgcg
     3061 ccattttctg gccatcgagg ggctgtcgcg gcagatattg accgagattc tcgacaccgc
     3121 cgaatctttt ctcacggttg gtgatcagcc ggtcaagaag gtgccgctgc tgcgcggcaa
     3181 gaccgtggtc aatctcttct tcgaggccag cacccgcacc cgcaccacct tcgagctggc
     3241 cgccaagcgg ctgt
//
LOCUS       DAORCQ010000159         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_26608_length_3150_cov_6.029079, whole genome shotgun sequence.
ACCESSION   DAORCQ010000159 DAORCQ010000000
VERSION     DAORCQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26608_length_3150_cov_6.029079"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKI23_11305"
     CDS             complement(<1..367)
                     /locus_tag="PKI23_11305"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNN37562.1"
                     /translation="MSRLSCFNRYLFRQLTQTTLVVILVIAGLDLLFALLGEMEDLSE
                     SYHLSDALLFVAMTSPRQLYEVTPFCLLIGVLIGLGNLARQSELVVMGSAGVSNWGIA
                     WSACRPVLLFMVATTLLGEW"
     gene            complement(364..1482)
                     /gene="lptF"
                     /locus_tag="PKI23_11310"
     CDS             complement(364..1482)
                     /gene="lptF"
                     /locus_tag="PKI23_11310"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNN37563.1"
                     /translation="MILLRYLFRQLVGATVAISSILLLVVISSRLSRYLADAASGKLA
                     PDLLFAMIGYRIPGVLEVVLPLGFFLGVLLTYGRLHVDREMTALAACGFSERRLLLYT
                     LLPALLTMVATAAVTLLYNPWGAAKAEQLLHRQLASNQFDALTSGRFQTDPHSGRVIY
                     IGQSENGDLREVFVAERLADVSQHRGAVDDAPVVVVATAGRLDYDPATEQRYLHLQAG
                     TRYQGWPGEPQMRVTDFAELGLRIATATGEYQVRKLDSIPTAQLFDSHEASAAATLLW
                     RLSPPLMAPIMVLIALPLSRVDPRQGRFARFLPAMLIYFTYLILLTTLRDLAAERGKL
                     PPMVIWAVHGLFLAAGVWMSLLGSGRAFLQSGRARATR"
     gene            1630..3135
                     /locus_tag="PKI23_11315"
     CDS             1630..3135
                     /locus_tag="PKI23_11315"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNN37564.1"
                     /translation="MEYKITKAAPEQQAAGMLVLGLLPKLQPSAAIKRLPPQTQEWIG
                     AILKQGDLPDKAGKSLLLHQVPGLKAERLLLLAQGEKAQISARDFLSIIRSVIGQAKA
                     TTAKSLLWSLDGLEVAGHDQAWMVRQIILGAETARYQYQATKGSNKPDDKQKLKKITL
                     QRSDLISPSQCEQLINQSSAMAHGVALARELGNLPPNLCTPSYLADQAKQLAQAHDAL
                     SLKVLNAKEMKALGMGALLAVAAGSAQPPKLIILEYRGATEDSAPHVLVGKGITFDSG
                     GISLKPGAAMDEMKFDMCGAASVLGVMQAIAELKAPINLVGIIAASENLPSGTALKPG
                     DVITSLSGKTIEVLNTDAEGRLVLCDALTYAERYNPKAVIDIATLTGACVVALGHHAS
                     GLFSNDQALTAALTASGEATHDRLWSLPLWKEYEEALESNFADLANVGGRSAGSITAA
                     CFLSRFTEKYPWAHIDIAGTAWISGGKEKGATGRPVPALLHYLLDQAEAQP"
BASE COUNT          699 a         1032 c          929 g          490 t
ORIGIN      
        1 cccactctcc cagcagagtc gtcgccacca tgaacagcaa taccggacgg caggccgacc
       61 aggcgattcc ccaattcgag acacccgcgc tgcccatcac caccaattca ctctgccggg
      121 ccaggttgcc cagcccgatc agcacaccga tcagcagaca gaacggcgtc acctcgtaca
      181 gttgcctggg cgaggtcatg gccacgaaga gcaatgcatc actcaaatgg tagctttcac
      241 tgagatcttc catctccccc agcaacgcaa acagcaggtc gagcccggcg atcaccagaa
      301 tgacgaccag agtggtctgc gtcagctgcc tgaacagata gcggttgaaa cagctcagcc
      361 tgctcatcgg gttgctctgg ctcggccact ttgaagaaaa gcgcgcccgc tcccgagcag
      421 actcatccat acaccggcag cgagaaacag cccatgcact gcccagatca ccatcggcgg
      481 cagtttgccc cgctcggccg ccagatcacg cagcgtggtg agcagaatca gataggtgaa
      541 gtagatcagc atggcgggca aaaagcgggc gaagcgaccc tgtcgtggat cgactcgcga
      601 caagggcagg gcgatcagta ccatgatcgg tgccatcagt gggggggaaa gacgccagag
      661 cagtgtggcc gcggcgctgg cttcgtggct gtcgaacaac tgcgccgtgg ggatcgaatc
      721 aagcttgcgg acctgatatt cgccagtggc agtggcgatg cgcagcccca gttcggcaaa
      781 gtcggtcacg cgcatctgtg gttcacccgg ccagccttgg tagcgggtcc cggcctgcag
      841 atgcagataa cgctgctcgg tggccgggtc gtagtcgagt cggccagcgg tggcgaccac
      901 caccaccggt gcatcatcga ctgcacccct gtgctgcgag acatcggcaa ggcgttcagc
      961 cacgaacact tcgcgcagat ctccgttttc gctctggccg atgtagatca cccgtccgct
     1021 gtgggggtca gtctgaaagc ggccactggt caatgcatcg aactgattgc tggccaactg
     1081 ccgatgcagc agctgctcgg ccttggcagc accccagggg ttgtacagca gcgtgacggc
     1141 ggcggtcgcc accatcgtca gcagtgccgg caacagggtg tagagcagca accgccgttc
     1201 gctgaagccg caggcggcca gcgcggtcat ctcgcgatcg acatgaagcc ggccataggt
     1261 gagcagcaca ccgagaaaaa acccgagcgg cagcaccacc tcgagcacac cgggaatgcg
     1321 gtaaccgatc atcgcgaaca gcagatcggg cgccagcttg ccactggcgg catcggccag
     1381 atagcggctc agacggctgc tgatcaccac cagcagcaga atcgagctga tcgcgacagt
     1441 ggcgccaacc agttgccgga atagatagcg aagtagaatc aaaagaggcg tcgcactgcc
     1501 agagtggtcg aagagccgcc attctacact gcatggattc aagacgcttg ttggcgcccg
     1561 gaggctcgat tagcattggt cgccctcgat accatccagc cgttttctct tcctcctcct
     1621 ggagtaccca tggaatacaa gatcaccaaa gccgctccgg agcagcaagc cgccggcatg
     1681 ctggttctcg gtctgctgcc aaaactgcaa cccagtgcgg caatcaagcg gcttccacca
     1741 cagacccagg agtggatcgg cgccatactg aaacagggtg atctgcccga caaggccggc
     1801 aagagcctgc tgctgcatca ggtgcccgga ctcaaggccg aacggctgtt actgctggcg
     1861 cagggcgaaa aggcgcagat cagcgccagg gattttctgt cgatcatcag aagcgtgatc
     1921 ggccaagcca aggcgaccac cgccaaaagc ctgctgtgga gcctcgacgg cctcgaggtg
     1981 gcgggacatg atcaagcctg gatggtgcgg cagatcatcc tcggtgccga aacggcccgc
     2041 taccaatacc aggccaccaa gggcagcaac aagcccgatg acaagcagaa actgaaaaaa
     2101 atcacgctgc aacgcagcga cctgatcagt ccatcgcagt gcgaacagtt gatcaatcag
     2161 agcagcgcga tggcccatgg cgttgccctg gccagggagc tcggcaacct cccgcccaac
     2221 ctctgcaccc ccagttacct cgccgaccaa gccaagcaat tggcacaggc gcacgatgcg
     2281 ctgtcactca aggtgctcaa tgccaaggag atgaaagccc tgggaatggg cgccctgctg
     2341 gcggtcgcag ccggcagcgc acaaccgccc aaattgatca ttctcgaata tcggggcgcc
     2401 accgaagaca gcgcccccca tgtgctggtc ggcaagggca tcaccttcga cagtggcggc
     2461 atcagcctca aacccggcgc agccatggac gagatgaagt tcgacatgtg cggcgccgcc
     2521 agtgtgctgg gcgtgatgca ggccatcgcc gaactgaagg cacccatcaa cctggtcggc
     2581 atcatcgccg ccagcgagaa cctgcccagt ggcactgccc tcaagccggg cgatgtcatc
     2641 accagcctct ccggcaagac catcgaagtg ctcaacaccg atgccgaagg gcgtctggtc
     2701 ctgtgtgatg ccctcaccta tgccgaacgc tacaacccca aggcggtgat cgacatcgcc
     2761 accctgaccg gcgcctgcgt cgtggcgctc ggccaccatg ccagcggcct gttcagcaac
     2821 gatcaggcgc tgaccgcagc cctgaccgcc agcggcgaag ccacccacga ccggctctgg
     2881 tcactgccat tgtggaaaga gtacgaagaa gcgctggaga gcaacttcgc cgacctggcc
     2941 aacgtcggtg gccgctcggc tggcagcatc accgccgcct gctttctctc ccgcttcacc
     3001 gagaagtatc cctgggccca catcgacatc gccggtaccg cctggatcag cggtggcaaa
     3061 gagaagggag cgaccgggcg cccagtgcct gccctgctcc actacctgct ggatcaggcg
     3121 gaagcgcagc cctgacggtg tccacccagg
//
LOCUS       DAORCQ010000160         3130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_26882_length_3130_cov_10.286829, whole genome shotgun
            sequence.
ACCESSION   DAORCQ010000160 DAORCQ010000000
VERSION     DAORCQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26882_length_3130_cov_10.286829"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..146
                     /locus_tag="PKI23_11320"
     CDS             9..146
                     /locus_tag="PKI23_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HNN37565.1"
                     /translation="MNTIGQPLYAKQEPRKFDRGTDLHTQSNPLPMCHRPGVLIKLVA
                     A"
     gene            146..424
                     /locus_tag="PKI23_11325"
     CDS             146..424
                     /locus_tag="PKI23_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37566.1"
                     /translation="MDVATLYEAARSAGLLTAVTVAGTTVHCAFRAPDETVLDGFALS
                     RDYQIDYPASWLTLAAGDTVEVAGNTYQVRDVRAIGDGTERRASLSQL"
     gene            435..860
                     /locus_tag="PKI23_11330"
     CDS             435..860
                     /locus_tag="PKI23_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37567.1"
                     /translation="MNSVRERVLREIVTRLASAIAPIPVLRMPAVPVTREASPALLLF
                     VDGDSITAHANHLVDRMLIVRLAVVARGADAFDVADQALVAAHAAMLADPNLGGLAIA
                     VREIDCEWEFDDADAGAVALPARYEIRYRTHAIDLTQTG"
     gene            865..1068
                     /locus_tag="PKI23_11335"
     CDS             865..1068
                     /locus_tag="PKI23_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37568.1"
                     /translation="MHIELLKPHTHAGKRLAVGDRLDLNDASARWLITQGTAKAATPA
                     TDSKPTRRDATSGVSTTAATQGD"
     gene            1071..1823
                     /locus_tag="PKI23_11340"
     CDS             1071..1823
                     /locus_tag="PKI23_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37569.1"
                     /translation="MAYFSGQGRVYIGARDDLGNPAGLTFVGNVPELKVSLSVDTIEH
                     QEAQSGQRLTDLQLIKTKKGEFACTLEELIATNLALALYGTTTTITPGTVTGELLPNP
                     VTPGSLYPLTMQNVSAVQIQDSDATPKTLPASQYSVNAKHGSLVVLDATSGGPYTEPF
                     TVDYAYGAAQSTAMFTQPLPERWIRFEGLNTADGNREVVIDLYRVAINPAKELSIITD
                     ELLKFELSGQVLADLTKPVGGDLGQFGRLVLL"
     gene            1823..2224
                     /locus_tag="PKI23_11345"
     CDS             1823..2224
                     /locus_tag="PKI23_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37570.1"
                     /translation="MDGFKTFPPEPVVVTLSGTALELTPIRLGELPRLLAVVRPLAEE
                     ITSDPDWMALLGRHGDAVLDLLAITTRRERAWINDLSLEDAVQLAAAVFEVNADFFVA
                     HVVPAIQGAAQRLAPTLRSLTNSAGTVPSPA"
     gene            2254..2409
                     /locus_tag="PKI23_11350"
     CDS             2254..2409
                     /locus_tag="PKI23_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37571.1"
                     /translation="MAYTLTQAQAFLDADGQIERQQLAQLLGIHAVAAQGEKRGIEQL
                     QRDLLKD"
     gene            2413..3054
                     /locus_tag="PKI23_11355"
     CDS             2413..3054
                     /locus_tag="PKI23_11355"
                     /inference="COORDINATES: protein motif:HMM:NF040424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6441 family protein"
                     /protein_id="HNN37572.1"
                     /translation="MRLSLTTTGLLDPRQLAAWSTERRRAIHTAVAKGMQSGGREVRD
                     AARSEMRSAFTVKRNSFISSMGVKVFDKKPELLPALLVGSKIPWLGLHEKGGTVSGNL
                     LIPLLPGRIGPKRFKAVIDGLMRSGNAFFIEKNGRVLLMAENIKENAGQLGRFKRAER
                     GRTGAKQIKRGQEIPIAVLVKRVDLKRRLNLAGGVQRALPALARAIQQELDKV"
BASE COUNT          581 a         1047 c          974 g          528 t
ORIGIN      
        1 acgagaccgt gaacaccatc ggccagccgc tgtatgccaa gcaggagcca cgcaagttcg
       61 accggggcac tgatctgcac acgcagagca acccgctgcc gatgtgccat cgccctggcg
      121 tgctgatcaa gctcgttgct gcctgatgga tgtcgcgacg ttgtacgagg cggcccgcag
      181 tgcaggactg ctgaccgccg tcacggtggc gggcaccacc gtgcactgcg ccttccgtgc
      241 cccggacgaa accgtgctgg atggttttgc actgtcgcgg gactaccaga tcgactaccc
      301 ggcgtcctgg ctgacgctgg ctgccgggga cacggtcgag gtggcaggca acacctatca
      361 ggtgcgcgac gtgcgcgcca tcggcgacgg caccgagcgt cgcgcctcgc tctcccaact
      421 ctgaggaact ccccatgaac tccgtccgcg agcgcgtctt gcgggagatc gtcacgcgcc
      481 tggcatctgc gattgccccg ataccggtgc tgcgcatgcc tgccgtgccg gtcacccgcg
      541 aggccagtcc agcgctgctg ctgttcgtcg atggcgacag cattaccgcc cacgccaacc
      601 acctggtcga ccggatgctg atcgtccggc ttgccgtggt ggcacgcggt gcggatgcct
      661 tcgacgtcgc cgaccaggcg ctggtcgcgg cccacgcggc aatgctcgcc gacccgaatc
      721 tgggcggtct ggccatcgcc gtgcgcgaga tcgactgcga atgggagttc gacgacgccg
      781 acgccggggc cgtcgcgctg cccgcccgct acgaaatccg ctaccgcacc cacgccattg
      841 atctcaccca aacaggatga acccatgcac atcgaactac tgaaacccca tacccacgca
      901 ggcaagcgcc tcgccgtggg tgatcgcctt gatctgaatg acgccagcgc ccgttggctg
      961 atcacacaag gcacggccaa agcggccacc ccagccaccg attccaaacc cacccgccgt
     1021 gatgccacgt ccggtgtttc cacaactgca gccacccaag gagactgaac atggcttatt
     1081 tttccggaca aggccgcgtc tacatcggcg cacgcgatga cctcggcaat ccagcaggac
     1141 tgaccttcgt cggcaacgtg cccgagctga aggtgtcgct gtcggtggac accatcgagc
     1201 accaggaagc gcagtcgggt cagcgcctga ctgacctcca gctcatcaag accaagaaag
     1261 gcgagttcgc ctgcacgttg gaagagctga tcgctaccaa cttggcgctc gcgctctacg
     1321 gcaccacgac cacgatcacc cccggcacgg taactggcga actactgccc aaccctgtca
     1381 ccccgggcag tctgtatccg ttgaccatgc aaaacgtgtc ggcggtgcag atccaggact
     1441 cagatgccac gcccaagacg ctcccggcca gccagtacag cgtcaatgcc aagcacggtt
     1501 cactggtggt gctggatgcc acgtcgggcg gcccgtacac cgagccgttc accgtcgatt
     1561 acgcctatgg cgcggcgcag agcacggcga tgttcaccca gcccttgccc gagcgctgga
     1621 ttcgcttcga ggggctcaac accgccgacg gcaaccgcga ggtggtgatc gacctctacc
     1681 gcgtggccat caacccggcc aaggaactct cgatcatcac ggacgaactg ctgaagttcg
     1741 agctttctgg ccaagtgctg gcggatctga ccaagccagt cggcggtgat ctcggtcagt
     1801 tcggtcgtct ggtgctgctg tgatggacgg cttcaagacc ttcccccctg agcctgtggt
     1861 cgtgacgctg tccggcaccg cgctggaact gacgccgatc cggctgggtg agttgccacg
     1921 gctgctggcc gtggtgcgcc cgctggccga ggaaatcacc agtgatccgg actggatggc
     1981 gctgctggga cggcacggcg atgccgtgct ggatttgctg gcgatcacca cccggcgtga
     2041 acgcgcgtgg atcaacgacc tgtcgctgga ggacgccgtg caactggccg ccgccgtgtt
     2101 cgaggtgaac gcggattttt tcgtggcgca cgtcgtcccg gcgattcagg gcgcggccca
     2161 gcgactcgcg ccgacgctgc gctcgctgac gaactcggct gggacagtgc cgtcgcccgc
     2221 ctgatccgcg ccgggcaccg cctgggcgac gtgatggcct acacgctcac tcaggcgcaa
     2281 gcctttctgg atgccgacgg acagatcgaa cggcagcaac ttgcccagct gctcggcatt
     2341 catgcggtgg cagcccaggg cgagaagcga ggcatcgaac aactgcaacg cgatctgctc
     2401 aaggactgac ccatgcgtct ctcgctcacc accaccggct tgctggaccc gcgccagttg
     2461 gcggcgtgga gtaccgagcg gcgtcgtgcc atccacaccg ctgtcgccaa gggcatgcaa
     2521 tcgggcgggc gtgaagtgcg tgatgcggcg cgatccgaga tgcgcagtgc cttcaccgtc
     2581 aagcgcaaca gcttcatctc ctcgatgggc gtgaaggtgt tcgacaagaa gcccgaactg
     2641 ctgcccgcct tgctggtggg cagcaagatt ccttggctcg gtctgcatga aaaaggcggc
     2701 acggtgagcg gcaatttgct gataccgctg ctgcccgggc gcatcggccc caagcgcttc
     2761 aaggcggtca ttgacggcct gatgcgctcg ggcaatgcct tcttcatcga gaagaacggt
     2821 cgcgtgctgc tgatggccga gaacatcaaa gagaacgccg ggcagctggg ccgcttcaag
     2881 cgtgccgagc gtggtcgtac cggggccaag cagatcaagc gtggccagga gattcccatc
     2941 gccgtgctgg tcaagcgcgt cgatctcaaa cgacgactga atctggcggg tggcgtgcaa
     3001 cgcgcactac ctgccttggc gcgggcgatt caacaagaac tggacaaagt ctgatggcaa
     3061 gcaatcgtgc ccaaatcctg atcagtgccg tcgaccagac caagaccgct ttcgactcga
     3121 tcaagcgggg
//
LOCUS       DAORCQ010000161         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_27749_length_3074_cov_6.772772, whole genome shotgun sequence.
ACCESSION   DAORCQ010000161 DAORCQ010000000
VERSION     DAORCQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27749_length_3074_cov_6.772772"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKI23_11360"
     CDS             <1..861
                     /locus_tag="PKI23_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HNN37573.1"
                     /translation="RFVLFHDKRHPREMGAAEIEAFLTHLAVVGKVSASTQNQAKSAL
                     LFLYRAVLNVDLPWLGDIASARQGKRLPVVLTVAEVQATLARVTGTTGLMVRLLYGSG
                     LRLMECARLRVKDVDFGQMQIVVRDGKGDKDRVTMLPRSLVEPLQAHLERVKALHESD
                     IEAGYGEVYLPQALDRKYPRAGREWGWQYVFPSTRRSVDPRSGAIRRHHADEKVLQRA
                     MKRAVREAGIVKPATPHTLRHSFATHLLQAGYDIRTVQELLGHTHVETTMLYTHVLNR
                     GGRGVVSPLD"
     gene            complement(1079..1169)
                     /locus_tag="PKI23_11365"
     tRNA            complement(1079..1169)
                     /locus_tag="PKI23_11365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1133..1135),aa:Ser,seq:gga)
     gene            complement(1211..1801)
                     /locus_tag="PKI23_11370"
     CDS             complement(1211..1801)
                     /locus_tag="PKI23_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNN37574.1"
                     /translation="MDALKALQGRQSAARLIDPAPDEAALAELFAAALRAPDHAGLRP
                     WRFLLVRGTARQQLGELFVRARQRRDPAASAAQIEKLRQAPLRAPLIMVAYSHLQPHP
                     KVPEIEQLLSTAAAVQNLLLAAHALGFGAIWRTGEMAHDPEVRAGLGLQQSDRIVGFI
                     YLGTAATPPKPLQPIDHATHFAEWLPGTIAFAASAG"
     gene            1943..2929
                     /locus_tag="PKI23_11375"
     CDS             1943..2929
                     /locus_tag="PKI23_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37575.1"
                     /translation="MQVTQLQLIYAAELVGFLLVLILILSLYGHNIRKHYFKAVGMLT
                     EWVAQEQQTAVQSISYDDYLNQAIEETEAHLRNVVGQSNELSMPIDTMEMKQFTALLR
                     AKFLRGERSAIQSSALHNEADWKQVEENMLLLVDEIEAHLAKRRRQQAVSQAAEPPPP
                     NHISASGLGEVEWVHEEKERSPAAGSSAETDAVAEELHTGLMGELTQKFTALEESKEQ
                     ALSLQERIMEQQRSLLDPDSASSSEELLQLLAQDSLDGHQALLRMERQIEQAEKILSR
                     AILATKGREEIDELQQRRDRLNRERMELKELQNCLVTLDRETEMASKRNGTA"
BASE COUNT          614 a          916 c         1016 g          528 t
ORIGIN      
        1 cggttcgtgc tgtttcacga caaacgccac ccgcgggaga tgggtgcagc cgagatcgag
       61 gcgtttctga cccacctcgc ggtggtgggc aaggtgtcgg cgtccaccca gaaccaggcc
      121 aagagcgcgc tgctgtttct ttatcgggct gtattgaatg tcgacttgcc gtggctgggc
      181 gacatcgcat cggcccggca gggcaagcgc ttgccggtgg tgctgacagt ggccgaagta
      241 caggcgacgc tggcgcgggt aaccggcacg accggcctga tggtgcggct cttgtacggc
      301 agcgggctgc ggttgatgga gtgcgcgcgg ctgcgggtga aggatgtgga tttcggacag
      361 atgcagatcg tggtgcgcga tggcaagggc gacaaggacc gcgtgacaat gctgccgcga
      421 tccctggtgg aacctctgca agcgcatctg gagcgcgtga aggcgctgca cgagagcgat
      481 atcgaagcgg gctacggcga ggtatacctg ccccaggcgc tcgatcgcaa atacccccgg
      541 gccggccggg aatggggctg gcagtatgtg tttccgtcca ccaggcggtc ggtcgatccc
      601 cgctccgggg ccatccggcg tcaccatgcg gatgaaaaag tactgcagcg ggcgatgaag
      661 cgagccgtgc gcgaggccgg catcgtcaaa cccgccaccc cgcacacgct gcgccacagc
      721 ttcgccacgc acttgttaca ggccggctat gacatccgca ccgtgcagga gctgctcggt
      781 cacacccacg tagaaaccac gatgctctac acccacgtcc tgaatcgggg tgggcgagga
      841 gtggtcagtc cgctcgactg agcggactga ggcgaagcac tgctttgcca gcgacgagcg
      901 ggcgagcgat gccacgcgag ccctggatcg aggagtggtg agtccgctcg atcagtcagt
      961 tgtagaccga tcacctggtt ggcggatgga gactcgtgtg gcgttgccga gacactcggg
     1021 cgcccaggac tggcgggatt cggccgatca gagcatgaga tcgcatcggc atggaatgtg
     1081 gcggagaggg agggattcga accctcgata cgctattaac gtatacacac tttccaggcg
     1141 tgctccttca accgctcgga cacctctccg caaacctggc tgaccctgcg gggtcaaaag
     1201 gtcggcaaat ctaccctgcc gaggccgcaa aagcaatcgt gccgggcagc cattcggcaa
     1261 aatgggtcgc atgatcgatg ggttgcagcg gttttggcgg ggtggccgcg gtgccgaggt
     1321 agatgaagcc gacgatccgg tcgctctgct gcaatcccag tccggcacgc acttccgggt
     1381 catgggccat ctcgccggtg cgccagatgg caccaaagcc gagtgcatgg gccgccagca
     1441 gcaggttctg caccgctgcc gccgtgctga gcagttgctc gatctcgggc accttggggt
     1501 gtggctgaag gtggctgtag gccaccatga tcagtggcgc ccgcagcggt gcctgacgca
     1561 gtttctcgat ctgcgccgcg ctcgctgccg gatcgcgcct ctggcgggca cgcacgaaca
     1621 gctcacccaa ctgctgacgc gccgtgccgc gcaccagcag aaagcgccag ggccgcaagc
     1681 cggcatgatc gggcgcgcgc agggcggcgg caaagagttc ggccagtgcg gcctcatcgg
     1741 gtgccgggtc gatcagtctg gccgccgact gccggccttg tagtgctttc agtgcatcca
     1801 tcgagccgct ccagtcactg ctgcctggcg caggccgtgc agcaaggaaa atatgatcca
     1861 tacttgatcc aatacctttt gcactgtttt gcggaaccac cgcacgaagt ccggagcaaa
     1921 gccctttttt gcgagcagga acatgcaggt cacccaactt caattgatct acgccgcaga
     1981 actggtcggg tttctgctgg tgctgattct gattctgtcg ctctacggac acaacatccg
     2041 caagcactat ttcaaggcgg tggggatgct gaccgaatgg gtggcgcaag agcagcagac
     2101 agccgtccag agcatcagct atgacgacta tctcaatcag gccatcgagg agaccgaagc
     2161 acatctgcgc aacgtcgtcg gccagtccaa tgagctgtcg atgcccatcg acaccatgga
     2221 aatgaagcag tttaccgcgt tgctgcgtgc caaattcctt cggggagagc gcagcgcaat
     2281 tcaatccagc gccttgcaca acgaggctga ctggaagcag gtcgaagaga acatgctgct
     2341 gctggtggat gagatcgagg cccatctggc caagcgacgt cgtcaacagg ccgtcagtca
     2401 ggctgccgag ccaccgccgc cgaatcacat ttcagccagc ggactgggcg aggtggaatg
     2461 ggtgcatgag gaaaaggagc gcagccctgc ggcaggttcc agcgctgaaa ccgacgcggt
     2521 tgccgaagag ctccacactg gcctgatggg ggaactgacg cagaagttca ctgccctgga
     2581 ggagtccaaa gagcaggcat tgtcactgca ggagcgcatc atggagcagc agcgcagcct
     2641 gttggatccg gattcagcca gctcctccga ggagctgttg caactgctgg cgcaggattc
     2701 gctcgacggc catcaggcgc tgctgcgcat ggagcggcag atcgagcagg ccgaaaagat
     2761 cctgtcgcgc gccattctcg cgaccaaggg gcgcgaggag atcgatgaac tgcaacaacg
     2821 gcgtgaccgc ctcaaccgcg agcgaatgga gttgaaggag ctgcagaact gcttggtcac
     2881 gctggaccgg gagaccgaga tggccagcaa acgcaacggc actgcatagt cggctgcgtc
     2941 gggcaccggt tccgagaaat cagctgctgt gccgatagag cgccagcagc gtcagcgctt
     3001 cacgctgctg ctgcgccccc actgccggtg gcaatgtggc attcggcaga cgcaggccgt
     3061 agggcacgcc ttcg
//
LOCUS       DAORCQ010000162         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_28273_length_3041_cov_3.984260, whole genome shotgun sequence.
ACCESSION   DAORCQ010000162 DAORCQ010000000
VERSION     DAORCQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28273_length_3041_cov_3.984260"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PKI23_11380"
     CDS             complement(<1..116)
                     /locus_tag="PKI23_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNN37576.1"
                     /translation="MTSLFDLTGKVALITGSSRGIGKAIAVAMAEQGARVVIS"
     gene            complement(148..1404)
                     /locus_tag="PKI23_11385"
     CDS             complement(148..1404)
                     /locus_tag="PKI23_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNN37577.1"
                     /translation="MTATPIQLIAAALFAVAILHTFSTKLFEHLAHTRPAHAGLWHLL
                     GEVEVVFGFWALILAAVLFIDDSSAAIHYIDSRNFTEPMFVFAIMVIAGSRPILQGVM
                     GVVRCLIGLLPLPGSLGSYIVVVGLIPLLGSFITEPAAMTLAALILADQLFSRGLSTR
                     LKYATLGVLFVNVSIGGTLTSYAAPPVLMVAGHWGWDSRFMLATFGWKAALAVAVNTL
                     GSALLFRRELARVPTTESRRGTVAVPWPLLLAHLAFLAGVVLFAHHPAIFMGLFLLFL
                     GVATAYPQHQDRLILREGLLVAFFLAGLVVLGGQQQWWLQPLLLGMSSDSVFVGATAL
                     TAITDNAALTYLGSLVDGLSDEFKYALVAGAVTGGGLTVIANAPNPAGMAILRGHFED
                     GAVHPLGLLLAALLPTLVAAAAFKFL"
     gene            complement(1475..2371)
                     /locus_tag="PKI23_11390"
     CDS             complement(1475..2371)
                     /locus_tag="PKI23_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNN37578.1"
                     /translation="MANQEIETIKTMMFSGRDPNAPTPSVAEMRQRYEGLGGAFPVGA
                     DVTIRTTALNGVPAEEITAEGADTRQALLYFHGGGYALGGFVTHRTLVSDLSRASRRR
                     AYLIDYRLAPENSFPAPVEDAVAAYRALLDLGYSSQQIVLGGDSAGGGLTLATMLALK
                     QQGLPLPAGGLCISPWADLEMTGSSHQSKADEDPMVRKPDLLNWTQIYLKGADPKNPL
                     ASPIHGDFSGLPPLLIQVGTAETLLDDARMVADRAKKAGVTVQLQEWQDMIHVWHHFA
                     AVVGDARKAIDEAGRFFQSLTA"
     gene            complement(2395..2997)
                     /locus_tag="PKI23_11395"
     CDS             complement(2395..2997)
                     /locus_tag="PKI23_11395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNN37579.1"
                     /translation="MNYCSQCAAPLIRKIPDGDDRLRHVCTACRHVHYQNPRVVTGTL
                     PVHGERVLLCKRAIAPRLGFWTLPAGFLENGESTDEGAVRETWEEALARVEINGLYTL
                     FNLPQIAQVHLFFRARLLNLDFAAGVESSEVRLFSEAEIPWQQLAFPVVSSTLRHYFA
                     DRLQGSYPLRIEDLRWERDPTRQPTAAHEATAQSPKARSE"
BASE COUNT          530 a          966 c         1046 g          499 t
ORIGIN      
        1 gagatcacca cccgtgcgcc ctgctccgcc atcgccacgg caatcgcctt gccgatgcca
       61 cggctcgatc cggtgatcag tgccaccttg ccggtcaggt caaagagcga tgtcatggca
      121 acctccgctg gttttgtcga tgtttgtcta caggaacttg aaggccgctg cggcgaccag
      181 cgtcggcagc agtgccgcca gcagcagtcc gagcggatgc accgcgccat cctcgaaatg
      241 gccgcgcagg atcgccatgc cggccgggtt gggcgcattg gcgatgacgg tcagaccacc
      301 accggtcacg gcgccggcga ccagtgcata tttgaactcg tccgacagcc cgtcgaccag
      361 cgagccgagg taggtcaggg cggcattgtc ggtgatggcg gtcagtgccg tggcgccgac
      421 aaagacgctg tcgctgctca tccccagcag cagcggttgc agccaccact gctgctggcc
      481 acccagcacc accaagcctg ccagaaagaa ggcgaccagc agcccttcgc gcaggatcag
      541 gcgatcctgg tgctgcgggt aggcggtggc gacgccgaga aagagcagaa acagccccat
      601 gaagatggcc ggatggtggg cgaacagcac gacaccggcg agaaaggcca ggtgtgccag
      661 cagcagcggc caaggcactg ccacggtgcc ccggcggctt tcggttgtcg gcactctcgc
      721 cagctcccgg cggaacagca gggcgctgcc cagcgtgttg acggccacgg caagcgccgc
      781 cttccagccg aaggtcgcca gcatgaagcg gctgtcccag ccccagtggc ccgccaccat
      841 cagcaccggg ggtgcggcat aagacgtcag ggtgccgccg atcgagacat tgacgaacag
      901 cacccccagc gttgcatatt tcagccgtgt cgacaggccg cgactgaaga gctgatcggc
      961 cagaatcagt gccgccagtg tcatggcggc cggctcggtg atgaaggagc ccagcagcgg
     1021 gatcagcccg accacgacga tgtagctgcc gaggctgccc ggcagcggca gcagaccgat
     1081 gaggcagcgc acgactccca tgaccccctg caggatcggt cggctgccgg cgatcaccat
     1141 gatggcgaac acgaacatcg gttcggtgaa gttgcgtgaa tcgatgtagt ggatggcggc
     1201 actgctgtca tcgatgaaca gcaccgccgc cagaatcagt gcccagaagc cgaagaccac
     1261 ctcgacctcg cccagcaggt gccacaggcc ggcatgggcg gggcgggtgt gggccagatg
     1321 ttcgaagagc ttggtcgaga aggtgtggag gatggccacc gcaaagagcg cggcggcaat
     1381 cagctggatc ggcgtggcgg tcatttctgt tcccgttcct gggtcatgcg acccgctgcc
     1441 cgccgggtga gcgggcaggg gtgcagtgcc cgattcaggc ggtgaggctc tggaagaacc
     1501 gtcccgcctc gtcgatggcc tttctggcat cgccgacgac cgcggcaaag tggtgccaga
     1561 catggatcat gtcctgccac tcctgcaact gcacggtgac cccggccttt ttggcgcgat
     1621 cggccaccat gcgcgcgtca tcgagcaggg tttcggcggt tccgacctgg atcagcagcg
     1681 gtggcaatcc actgaagtcg ccatggatcg gcgaggccag cgggttcttc ggatcggcgc
     1741 ccttcaggta gatctgggtc cagttcagca ggtcgggctt gcgcaccatc gggtcttcat
     1801 cagccttgct ctggtggctg ctgccggtca tctccagatc ggcccacggc gagatgcaga
     1861 gtccgccggc cggcagcggc agtccctgct gcttgagcgc cagcatggtg gccagcgtca
     1921 ggccgccacc ggccgagtcg ccgcccagta cgatctgctg gctgctgtag ccgaggtcga
     1981 gcagggcgcg gtaggcggcc acggcatcct cgaccggcgc cggaaagctg ttctctggtg
     2041 ccagccggta gtcgatcaga taggcgcggc gccggctcgc ccgtgacaga tcggagacca
     2101 gcgtgcggtg ggtgacgaag ccgcccagcg cgtagccgcc accgtggaag tagagcagtg
     2161 cctgacgggt gtcggcgccc tcggcggtga tctcctcggc gggcacgcca ttcagcgcag
     2221 tggtgcggat ggtgacatcg gcgccgaccg gaaaggcgcc gccgagccct tcgtagcgct
     2281 ggcgcatctc tgccacactg ggggtgggtg cgttggggtc gcgtcctgaa aacatcatcg
     2341 tcttgatggt ttcgatttcc tgattggcca tgggcgtcgt ctccttgggg gttgttattc
     2401 tgaacgggct ttcggactct gggcggtggc ctcatgcgcg gcggtcggct ggcgcgtcgg
     2461 gtcgcgctcc cagcgcagat cctcgatgcg cagtggatag ctgccctgca ggcggtcggc
     2521 gaagtagtga cgcagcgtgc tgctcaccac cggaaaagca agctgttgcc acggaatctc
     2581 cgcctcgctg aagagccgca cctcgctgct ctcgacgccg gcggcaaagt cgaggttgag
     2641 caggcgggcg cgaaagaaca ggtgcacctg ggcgatctgc ggcaggttga acagcgtgta
     2701 gaggccattg atctcgaccc gcgccagcgc ctcctcccag gtttcgcgca cggcaccctc
     2761 atcggtcgac tcgccgttct cgagaaaacc ggccggcagc gtccagaaac ccagacgggg
     2821 ggcgatggct ctcttgcaca gcagcacccg ttcgccatgg accggcaggg tgccggtgac
     2881 gacgcggggg ttctggtagt gcacatgccg gcaggcggtg cagacatggc gcaggcggtc
     2941 gtcgccgtca ggaatcttcc tgatcagcgg agcggcgcac tggctgcagt agttcatgct
     3001 gggggcatcc cgggcgagcg ttgccgggat gctacacgtt c
//
LOCUS       DAORCQ010000163         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_28870_length_3005_cov_5.270169, whole genome shotgun sequence.
ACCESSION   DAORCQ010000163 DAORCQ010000000
VERSION     DAORCQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28870_length_3005_cov_5.270169"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKI23_11400"
     CDS             <1..490
                     /locus_tag="PKI23_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="12-oxophytodienoate reductase"
                     /protein_id="HNN37580.1"
                     /translation="EIIEACRTAVGPDLPIILRFSQWKQQDYEAKLAHTPEELEQFLT
                     PLARAGVDIFHASTRRFWQPEFEGSELNLAGWTKKLTGKPTITVGSVGLDDDFIASFM
                     GKGAGTAGIDQLLERMQHGEFDLVAVGRALLLDPNWARKLHRGEVDQFQPFNPAALAT
                     LS"
     gene            complement(505..1590)
                     /locus_tag="PKI23_11405"
     CDS             complement(505..1590)
                     /locus_tag="PKI23_11405"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HNN37581.1"
                     /translation="MGSSRNLEPFTGRTGRRLRLATFALLLATAALAAEPGRATPEPW
                     TVNFYLENDLFVNTDNDYTNGLRLSFISPDLDNYLDDPTLPQWVREINRQLAFMHGSL
                     HGVDRNLVLAIGQAIYTPADPVPAGLIVDDRPYAGWLYGMVGYQTKKRDRLDSLELHL
                     GVVGPASQAEAAQDLIHGLRHIEKFNGWHNQLDNEPGLIALYEHKDRLFRHRLRDGFG
                     GDLIAHGGIALGNVASYLNLGGTLRLGYNVPDDFGSSPIRPGGDNSAPGSSARRYLNS
                     DHAWGSHLFASVDGRLVGRDIFLDGNTFEESHHVHKERFTGDLSVGAVFHFQRYRVVF
                     SRVFTAQEFEEQRRSHSFGSLMLSYFL"
     gene            1670..2626
                     /gene="rssA"
                     /locus_tag="PKI23_11410"
     CDS             1670..2626
                     /gene="rssA"
                     /locus_tag="PKI23_11410"
                     /inference="COORDINATES: protein motif:HMM:NF007623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HNN37582.1"
                     /translation="MFNSSTRPRLGLALGAGAARGWAHIGVIEALEEAGIVPEVVAGC
                     SIGALVGAAYVNGRLDDLKQWVTSLSRWDVARFFELKWSLNGVADSARLRDALIHHVA
                     DEQTAIETLEKRFASVATNLMNGQEIWLQHGSLLEAVWASIALPGLFPPWHRHGEWLV
                     DGGMVNPVPVSLCRVLRADVVIAVGLNEGLLRRRQVGLWSPSAAEEVTQSDSTSLLDT
                     LSRTLRERAALLFPQPAEVDRPAMPGLFETLAISLNIMQERIFRSRMAGDPPEVVMTP
                     WLGEIGILEFYRASEAIEIGRACVARMLPELRETLAGFGHPS"
     gene            2646..>3005
                     /locus_tag="PKI23_11415"
     CDS             2646..>3005
                     /locus_tag="PKI23_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNN37583.1"
                     /translation="MNRTRWIDATPYVTKDGSTIRELMHPALHGNQGQSLAEARLPPG
                     GSTLLHRHLRSEEIYHVTEGAGLMQLGEQRLALTPGDTVAIPAGVPHALTNPGEVELV
                     LLCLCVPPYSHEETELLV"
BASE COUNT          519 a          909 c         1017 g          560 t
ORIGIN      
        1 ggagatcatc gaggcctgcc gtaccgcggt ggggcccgat ctgccgatca tcctgcgctt
       61 ctcgcaatgg aagcagcagg attacgaggc caaactggcg cacacgcccg aggagctgga
      121 gcagtttctg acgccactgg cgcgggccgg tgtcgacatc ttccatgcca gtacccgccg
      181 cttctggcag ccggagttcg aaggctccga gctcaacctg gccggctgga ccaaaaagct
      241 gaccggcaag ccgaccatca ccgtcggcag cgtggggctc gatgacgact tcatcgcctc
      301 gttcatgggc aagggtgctg gtacggctgg catcgaccag ttgctggagc ggatgcagca
      361 cggcgaattc gatctggtgg cggttggccg ggcactgctg ctcgacccga actgggcgcg
      421 caagctgcac cgcggcgagg tcgaccagtt ccagcccttc aacccggcgg ccttggcgac
      481 gctgagctga aggcgaggga cgggttacag aaaataggag agcatcagcg agccaaagga
      541 gtggctgcgc ctctgctctt cgaactcttg agccgtgaag acgcgcgaga agaccactcg
      601 ataacgctgg aagtgaaaca cggcaccgac cgaaagatcg ccggtgaagc gctccttgtg
      661 cacatggtga ctctcctcaa aggtgttgcc atccagaaag atgtcgcggc cgaccagtcg
      721 cccatccacc gaagcgaaca ggtggctgcc ccaggcgtgg tcgctgttca gatagcgtcg
      781 ggcgctgctg cccggtgcgc tgttgtcgcc acccgggcga atcggtgaac tgccgaagtc
      841 atccggcacg ttgtagccaa ggcgcaatgt gccccccagg ttgaggtagc tggcgacgtt
      901 gccgagcgcg atgccgccat gggcgatcag gtcaccaccg aagccgtcgc gcagacggtg
      961 tctgaacagc cgatccttgt gctcgtacag tgcgatcagc ccgggttcgt tgtcgagctg
     1021 attgtgccag ccgttgaact tctcgatgtg gcgcagaccg tggatcagat cctgcgccgc
     1081 ctcggcctgc gatgcggggc cgacgacgcc gaggtgcagt tccagtgaat cgagccggtc
     1141 gcgcttcttg gtctgatagc cgaccatgcc atagagccag ccggcatagg ggcggtcgtc
     1201 gacgatcagc ccggcgggca ccgggtcggc aggggtgtag atggcctgac cgatcgcgag
     1261 caccagattg cggtcgacgc catgcagcga gccatgcatg aaggccagtt gacggttgat
     1321 ctccctgacc cactgcggca gggtcggatc atccaggtag ttgtcgagat cgggtgagat
     1381 gaacgacagg cgcaggccat tggtgtagtc gttgtcggtg ttgacgaaaa gatcgttctc
     1441 aagataaaag ttgactgtcc agggctccgg ggtggctctg ccgggttcgg cggccaaagc
     1501 tgccgtggcc agcagcagcg cgaaggttgc cagtcgcagc cgtcggcccg tgcggccggt
     1561 gaagggttca aggtttcgac tgctgcccat ctctgtcact cctgccggtg ggtgccgctg
     1621 ctcgatcgtt gtctgccgcg ccattttccg acaattgtaa aaggttgcca tgttcaattc
     1681 gtccaccagg ccacggctgg gtctggccct gggagccggt gccgcccgtg gttgggctca
     1741 tatcggcgtc atcgaggcgc tggaggaggc cggcattgtg cccgaagtgg tggccggctg
     1801 ctcgatcggc gcgctggtgg gggccgccta tgtcaatggc cgactggatg atctgaagca
     1861 gtgggtcacc tcgctgtcac gctgggatgt ggcacgcttt ttcgaattga agtggtcgct
     1921 gaacggggtg gccgattctg cgcggctgcg tgatgcactg atccaccatg tggccgacga
     1981 acagaccgcc atcgaaacgt tggagaagcg ttttgccagc gtcgccacca acctgatgaa
     2041 tgggcaggag atctggttgc agcacggctc cctgctggag gcggtctggg catcgatcgc
     2101 cctgccaggg ctctttccgc cctggcatcg ccatggcgag tggctggtcg atggtggaat
     2161 ggtcaatccg gtgccggtct cgctctgccg ggtgctgcgc gccgatgtgg tgatcgcggt
     2221 gggcctcaac gaagggctgc tgcgacgcag acaggtcggg ctgtggtcgc cctccgccgc
     2281 tgaggaggtg acgcagagcg attccaccag tctgctcgac acactgtcgc gcacgctgcg
     2341 cgaacgggct gcgctgctgt ttccacagcc agccgaggtc gacaggccag ccatgccggg
     2401 attgttcgag acccttgcca tctcgctcaa catcatgcag gagcgcatct ttcgcagccg
     2461 catggcgggc gatccgcccg aggtggtcat gaccccgtgg ctgggcgaga tcggcattct
     2521 ggagttttac cgtgccagcg aggcgatcga gatcggccgc gcctgcgttg cccgcatgct
     2581 gccggagctg cgcgagacgc tggccggttt tggccaccca tcctgaccat tccactggag
     2641 ccatcatgaa tcgaacccgc tggatcgacg ccacgcccta cgtcaccaag gatggttcca
     2701 ccattcgtga gctgatgcat ccggccctgc atggcaatca ggggcagagt ctggccgagg
     2761 cgcggctgcc gccgggtggg tcgacgctgc tgcaccgcca tctgcgcagc gaggagatct
     2821 accatgtgac cgagggggcc ggcctcatgc agttgggcga gcagcggctg gcactgacac
     2881 cgggtgacac cgtcgccatt ccggcaggcg tgccccatgc gttgaccaat ccgggcgagg
     2941 tcgagctggt gctgctctgt ctctgcgtac cgccctacag ccatgaagag accgagttgc
     3001 tggtg
//
LOCUS       DAORCQ010000164         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_29389_length_2971_cov_6.180384, whole genome shotgun sequence.
ACCESSION   DAORCQ010000164 DAORCQ010000000
VERSION     DAORCQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29389_length_2971_cov_6.180384"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /gene="ettA"
                     /locus_tag="PKI23_11420"
     CDS             <1..1381
                     /gene="ettA"
                     /locus_tag="PKI23_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNN37584.1"
                     /translation="TKALLDRFNEISNAFADPDADFDTLLAEQAELQDKIDAAGAWEL
                     DRTLDVAADALRLPPWEADVTRLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESV
                     AWLERFLHDYPGTVIAVTHDRYFLDNVASWILELDRGHGIPWEGNYSSWLAQKEKRLE
                     QEEKQESAHRKAMQAELEWVRSNPKGRTAKSKARLARFDELASQEFQKRNETQDLYIP
                     PGPRLGDLVIKGEGLRKAFGDRLLFDDLNFQLPPGGIVGIIGPNGAGKTTLFRILAGQ
                     EQPDAGTLRFGETLQLAYVDQSRQSLDDKHTVWEEISGGHDILRVGSYETPSRAYVGR
                     FNFKGTDQQKRIGELSGGERNRVHLAKVLKSGGNVLLLDEPTNDLDVETLRALEEALL
                     TFPGCAVVISHDRWFLDRIATHILAFEGDSKVVWFEGNYADYEADRKRRLGVDADQPH
                     RLKYRPLSA"
     gene            1467..2108
                     /locus_tag="PKI23_11425"
     CDS             1467..2108
                     /locus_tag="PKI23_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014340397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNN37585.1"
                     /translation="MIELIDFILHVDRHLIEFVQQYGAWIYLLLFMIIFVETGLVIMP
                     FLPGDSLLFAAGALSATGALHPGLLTGLLFVAAVLGDNLNYLIGRRAGKAVFERDSRW
                     IRRDYLERTQLFFDKHGGKSVILARFVPIVRTFTPFLAGVGKMPYGEFLAYNLLGGAL
                     WVAGFVLLGYYFGNLPIIRSNFSLVIFLIIGISLLPALIEVWRQRRPGKRANP"
     gene            2223..2861
                     /locus_tag="PKI23_11430"
     CDS             2223..2861
                     /locus_tag="PKI23_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNN37586.1"
                     /translation="MLKLYGFAVSNYYNIVKHYLLEKEIPFEAVTVAPSQEPALLAKS
                     PMGKVPFIETERGILTETSVILDYLEACHPQPALYPADPFARARMQQIIKTIELYIED
                     PAHRMIGALFGREVPESVKESSTALLRRGLPALGRIASLSPYLCGSELTAADIFAFYA
                     LKLSETACRRVLDWDIIAEVPGLATMMEKVAERDVTKRVLADNIAALKAMSG"
BASE COUNT          551 a         1049 c          870 g          501 t
ORIGIN      
        1 gaccaaggcc ctgctcgatc gcttcaacga gatcagcaac gcctttgccg accccgatgc
       61 cgacttcgat accctgctgg ccgagcaggc cgagttgcag gacaagatcg acgccgccgg
      121 cgcctgggag ctcgaccgca ccctggacgt ggccgccgac gcactgcgcc tgccgccatg
      181 ggaggccgat gtgaccaggc tctccggtgg cgagaagcgc cgcgtggcgc tgtgccggct
      241 gctgctctcc aaaccggaca tgctgctgct cgatgagccc accaaccacc tcgacgccga
      301 atcggtggcc tggctcgaac gctttctgca cgactacccc ggcaccgtca ttgccgtcac
      361 ccacgaccgc tactttctcg acaacgtcgc cagctggatt ctggagctcg accgcggtca
      421 cggcatcccc tgggaaggca actactccag ttggctggcg cagaaagaga agcggctgga
      481 gcaggaagaa aagcaggagt cagcccaccg caaggcgatg caggccgagc tcgaatgggt
      541 gcgcagcaat cccaaggggc gcaccgccaa gagcaaggcg cggctggccc gcttcgacga
      601 actggcctca caggagttcc agaagcgcaa cgaaacccag gatctctaca tcccgcccgg
      661 accccgcctt ggcgacctgg tgatcaaggg cgaagggctg cgcaaggcct ttggcgaccg
      721 gctgctgttc gatgacctga actttcaact gccgccgggc ggcatcgtcg gcatcatcgg
      781 ccccaacggc gccggcaaga ccacgctgtt ccgcatcctg gccggtcagg agcagcccga
      841 cgccggcacg ctgaggttcg gtgagaccct gcagctcgcc tatgtcgacc agagccggca
      901 gagcctcgat gacaaacata cagtgtggga ggagatctcc ggcggccatg acatcctgcg
      961 cgtcggcagc tacgaaacgc catcacgcgc ctatgtcggc cgcttcaact tcaagggcac
     1021 cgaccagcag aagcggatcg gcgaactgtc gggcggcgag cgcaaccggg tccatctggc
     1081 caaggtgctc aagagcggcg gcaacgtgct gctgctcgac gaaccgacca acgacctcga
     1141 cgtcgagacc ctgcgggcgc tcgaagaggc gctgctgacc tttcccggct gcgccgtggt
     1201 catctcccac gaccgctggt tcctcgaccg catcgccacc cacatcctcg ccttcgaggg
     1261 cgacagcaag gtggtctggt tcgagggcaa ctacgccgac tacgaggccg accgcaagcg
     1321 ccgtctcggt gtcgacgccg accagccgca ccgcctcaaa taccggccat tgagcgcctg
     1381 atcactgtcc ggctgtcgca gtcgaccgac cagcggcagc cggccgtgcc agccacaccc
     1441 cattcacttg cgttgagcag caccccatga tcgagctgat cgatttcatc ctgcatgtcg
     1501 accgtcacct gatcgaattc gtccagcagt acggcgcctg gatctacctg ctgctgttca
     1561 tgatcatctt cgtcgagacc ggactcgtca tcatgccgtt tctgcccggt gacagcctgc
     1621 tgttcgccgc cggcgcgctc agcgccaccg gcgcgctgca cccggggctg ctcaccggac
     1681 tgctgttcgt cgccgcggtg ctcggcgaca acctcaacta cctgattggc cgccgcgccg
     1741 gcaaggcggt cttcgaacgc gactcgcgct ggattcgacg cgactatctg gagcgaaccc
     1801 agcttttttt cgacaagcat ggcggcaagt cggtgatcct ggcccgcttc gtcccgatcg
     1861 tgcgcacctt cacccccttt ctggccggtg tcggcaagat gccctacggc gagtttctcg
     1921 cctacaacct gctgggcggc gcattgtggg tcgccggctt cgtgctgctc ggttactact
     1981 tcggcaacct gccgatcatc cgcagcaact tcagcctggt gatctttctg atcatcggca
     2041 tcagcctgct gcccgcgctg atcgaggtgt ggcgccagcg ccgaccaggc aagcgcgcca
     2101 atccatagca cccttgcgcg cctgccggaa ccacacagca tccgttacaa tcgggcggtc
     2161 gcctgtcggc agtgaaatgg ctcactgacc gacggcggct ccacccataa ggagaatctc
     2221 ccatgctcaa gctctacggc tttgcggtca gcaactacta caacatcgtc aaacactacc
     2281 tgcttgaaaa agagattccc ttcgaagccg tgacggtcgc cccgagccag gagcctgcgc
     2341 tgctggccaa gagcccgatg ggcaaagtgc ccttcatcga gaccgaacgg ggcattctca
     2401 ccgagaccag cgtcattctc gactacctcg aagcctgcca tccgcagccg gcgctctacc
     2461 ccgccgaccc cttcgcgcgg gccaggatgc agcagatcat caagaccatc gagctctaca
     2521 tcgaagaccc ggcccaccgc atgatcggcg cgctcttcgg tcgcgaggtg cccgaatcgg
     2581 tcaaggagag ctccaccgca ctgctgcgcc gcggcctgcc ggcactcgga cgcatcgcct
     2641 cactctcgcc ctacctctgc ggcagcgaac tgaccgccgc cgacatcttt gccttctacg
     2701 ccctgaagct gagcgaaacc gcctgccgcc gcgtgctcga ttgggacatc atcgccgaag
     2761 tgccgggcct ggccacgatg atggagaagg tcgccgagcg cgacgtgacc aagcgcgtgc
     2821 tggccgacaa catcgcggcg ctcaaggcga tgagcggctg agcgcgcatt cgacagacca
     2881 tggtcagcgc cctggccatg gtttctgtcc gcgtcatcca catccgaggc atgatcttcg
     2941 gcttctgaac gggcgcgtca tccatgcgga a
//
LOCUS       DAORCQ010000165         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_30915_length_2885_cov_6.299647, whole genome shotgun sequence.
ACCESSION   DAORCQ010000165 DAORCQ010000000
VERSION     DAORCQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30915_length_2885_cov_6.299647"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /gene="rfaE2"
                     /locus_tag="PKI23_11435"
     CDS             <1..760
                     /gene="rfaE2"
                     /locus_tag="PKI23_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNN37587.1"
                     /translation="ALLVTRGGQGMLLVPREGDPINLRAQAREVYDVTGAGDTVIAVL
                     AAALGGGESLERAMVLANRAAGLVVGKLGTATVSADELLRADAAAETPPRGVMSESAL
                     QAMVEAAQARGERVVFTNGCFDLLHTGHVSYLAAARELGDRLIVAVNSDESVRRLKGT
                     GRPINPLSQRMAVLGALAAVDWVVAFSEETPERLIRLLKPDLLVKGGDYRPEQIAGAD
                     FVRAQGGEVRVLDYVEACSTTAMIEKMAARQGAE"
     gene            805..2079
                     /gene="waaA"
                     /locus_tag="PKI23_11440"
     CDS             805..2079
                     /gene="waaA"
                     /locus_tag="PKI23_11440"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNN37588.1"
                     /translation="MLRGLYTLLLALLLPALLLRLLWRSRAEPGYRQRIAERFGRCTL
                     KVDQPCIWIHAVSFGETMAARPLVEALLERFPQGRLLITSTTATGSARVRTLFGDRVE
                     HCYLPYDLPIFLDPFLRRARPALAIFMETELWPNLLARLQQLQVPAILANGRLSQRSA
                     QGYARIGGVSRAMMQRMTLVAAQHRQDGERFVELGLPAERMVVTGNLKFDHAVDPRLV
                     SEAERLRVHWSLGGRPVWVAGSTHEGEEPLLLSVHRQLLEKLPDTLLILVPRHPQRFD
                     AVARLVDEMGFKQCRRSSGARVTAATQLLLGDTMGELQLWYALADLAFVGGSLVPVGG
                     HNLLEPAALGRPVLSGPHLFNFNETRALLLQAGAARVVEDQQALLEQLLLLLADRVRR
                     TQMGGAGCAVVEGNRGATLRLLALLEHYLPQV"
     gene            complement(2090..>2885)
                     /locus_tag="PKI23_11445"
     CDS             complement(2090..>2885)
                     /locus_tag="PKI23_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNN37589.1"
                     /translation="VVNRINAENQVEVSFRVLEAITGVRYNRLSTLSPTFPIVRPEPA
                     QPEPWIDQVLANNYGLKSLEQSVEAARQNVQVKRAAHYPTVSLFGRYQDDYTNNAPEL
                     VVGTQRFQLRNTDQDTSSYGLELKMPLFTGGLISSQARQAAYEHEAANENLETVRRNL
                     TQTTRILHSQLLTDVERVKARQQAVTSSESALKATEVGYEVGTRNIVELLQSQRMLYA
                     TQREYARSRYDYVVRYLQLKQATAQLAPTDLLDLNAWLQAPGSVKP"
BASE COUNT          420 a          879 c         1047 g          539 t
ORIGIN      
        1 cgcactgctg gtgacccgtg gcgggcaggg aatgctgctg gtaccgcggg agggcgatcc
       61 gatcaacctg agggcgcagg cgcgggaggt ctacgatgtc accggtgccg gcgacaccgt
      121 gatcgcggtg ctggcggcag cgctcggcgg cggcgaatcg ctcgaacggg ccatggtgct
      181 ggccaaccgt gccgccggcc tggtggtcgg caaactcggt accgccaccg tcagcgccga
      241 tgaactgctg cgggccgacg cggcggccga gacgccaccg cgcggggtga tgagcgaatc
      301 ggccctgcag gccatggtgg aagccgcgca ggcgcgtggc gagcgggtgg tcttcaccaa
      361 tggctgtttc gatctgctcc ataccggtca cgtcagttat ctcgctgcgg cccgtgagct
      421 gggggatcgt ctgatcgtgg cggtcaacag cgatgagtcg gtgcgtcgac tcaagggcac
      481 aggccggccg atcaacccgc tgtcacagcg gatggcggtg ctcggcgcgc tggccgcggt
      541 cgactgggtg gtcgccttca gcgaggagac gccggagcgg ctgatccggt tgctgaagcc
      601 cgatctgctg gtgaagggcg gtgattaccg gccggagcag attgccggcg ccgacttcgt
      661 gcgcgcgcag ggcggcgagg tgcgggtgct cgactacgtc gaagcctgct cgaccacggc
      721 gatgatcgaa aagatggccg cgcgacaagg tgccgagtga gcgatgccga actgccgcaa
      781 ccggtctcga ccctgagcga acggctgctg cgtgggctct acacgctgct gctggcgctg
      841 ctgctgccgg cgctgctgct gcgactgctc tggcgttcgc gggccgagcc gggttaccgg
      901 cagcgcatcg ccgaacggtt tggccgttgc acgctcaagg tggatcagcc ctgcatctgg
      961 attcatgcgg tctcctttgg cgaaaccatg gcggcccggc cgctggtcga ggcgctgctc
     1021 gagcgctttc cgcagggtcg gttgctgatc acctcgacca ccgccaccgg atcggcgcgg
     1081 gtgcgcactc tgttcggcga tcgtgtcgag cactgttatc tcccctacga cctgccgatc
     1141 tttcttgacc cctttctgcg tcgtgcccgc ccggcgctgg cgatcttcat ggagaccgag
     1201 ctgtggccca atctgctggc gcgtctgcag cagttgcagg tgccggccat tcttgccaac
     1261 ggccggctct cgcagcgctc ggcgcagggc tatgcccgca tcggcggagt gagccgcgcg
     1321 atgatgcagc gcatgacact ggtggcggcg cagcatcggc aggatggtga gcgcttcgtc
     1381 gagctgggtc tgccggccga gcggatggtg gtgaccggca acctcaagtt cgatcatgcc
     1441 gtcgatccgc ggctggtgag cgaggccgaa cggctcaggg tgcactggtc actgggggga
     1501 cgaccggtct gggtggccgg cagcacccat gagggcgagg agccgctgct gctgagcgtg
     1561 caccgccagt tgctggagaa actgcccgat acgctgctga ttctggtgcc gcgccatccg
     1621 cagcgcttcg atgccgtcgc ccggctggtc gatgaaatgg gcttcaagca gtgtcgccgc
     1681 tcgagcggcg cgcgggtcac cgctgccacc cagctgctgc tgggcgacac catgggcgag
     1741 ttgcagctgt ggtatgcgct ggccgacctg gccttcgtcg gcggcagtct ggtgccggtg
     1801 ggaggacaca acctgctcga gcccgccgcg ctcggtcggc cggtgttgag tggtccgcat
     1861 ctgttcaact tcaacgagac cagggccttg ctgttgcagg ccggtgccgc acgggtggtc
     1921 gaggatcagc aggcgctgct tgaacagctc ctgctgctgt tggccgatag ggtgcggcgc
     1981 acgcagatgg gcggcgccgg ctgtgcagtg gtcgagggca accgcggtgc caccctgcgg
     2041 ctgctggcgc tgctggagca ctacctgccg caggtgtgac cgaagctgat cagggcttga
     2101 cgctgccggg cgcttgcagc caggcgttga gatcgagcag gtcggtcggc gccagctggg
     2161 cggtcgcctg cttcagttgc agatagcgga ccacgtagtc atagcgtgag cgggcatatt
     2221 cgcgctgggt ggcgtagagc atccgttgcg actgcagcag ttcgacgatg ttccgcgtac
     2281 ccacttcata gcccacctcg gttgccttca gcgcactctc gctcgaggtg acagcctgct
     2341 gacgcgcctt gacccgctcg acatcggtca gcaactggct gtgcaggatg cgggtggtct
     2401 gggtcaggtt gcggcgaacg gtttcgaggt tttcgttggc ggcctcatgc tcgtaggcag
     2461 cctgccgcgc ctgggaggag atcagcccgc cggtgaacag cggcattttc agttccaggc
     2521 cgtaggagga ggtgtcctga tcggtgttcc tgagctggaa tctttgagtg cccacaacca
     2581 gttcaggtgc attgttggtg tagtcatcct gatagcggcc gaacagcgaa acggtcggat
     2641 agtgggcggc gcgcttgacc tggacgttct gcctggcggc ctcgacgctc tgctcgaggc
     2701 tcttcagccc gtagttgttg gccagaacct gatcgatcca gggttcgggc tgtgccggtt
     2761 cgggccggac gatcgggaag gtcgggctca gcgtcgacag gcggttgtaa cgcacgccgg
     2821 tgatggcttc gagcacccgg aaggagacct cgacctggtt ctcggcattg atgcggttga
     2881 ccacg
//
LOCUS       DAORCQ010000166         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_31063_length_2877_cov_5.684621, whole genome shotgun sequence.
ACCESSION   DAORCQ010000166 DAORCQ010000000
VERSION     DAORCQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31063_length_2877_cov_5.684621"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKI23_11450"
     CDS             complement(<1..388)
                     /locus_tag="PKI23_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNN37590.1"
                     /translation="MSLLSSLPLLPQIILACLLGGVLSIAAAALIMFGLPRKWLAFTV
                     SFSTGLLLAMAMLHLLPEALEMGLTPHEVFPLLLGGILGFFALEKFALWRHAHHGTGD
                     ADSHPGEHQCTDHTHSHHHAHGQGGET"
     gene            589..1116
                     /locus_tag="PKI23_11455"
     CDS             589..1116
                     /locus_tag="PKI23_11455"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNN37591.1"
                     /translation="MNCVWKRALAALLILASPLAVADDRPVTTVPNVDLERYLGRWYE
                     IGSFPMFFQRNCIGETTADYALQEGGSISVRNRCRSEDGFIEAEGRATPVAGSGNARL
                     KVSFFWPFSSDYWIIGLDDAYRWAVVGNPNRKYLWILSRTPVLDPAALEQARMIAAAQ
                     GFDLAQLRMTRQLAE"
     gene            complement(1185..2558)
                     /locus_tag="PKI23_11460"
     CDS             complement(1185..2558)
                     /locus_tag="PKI23_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNN37592.1"
                     /translation="MQMPIYVQGAWFAGALLFIFGLKGMSSPASAQRGIVIAGYGMLL
                     AIAAVFFIPGLQNLGLMALALALGGAAAWISGKQVKMTDMPQMVAIYNGMGGGAAAAI
                     AAVEFARGDTHGMATTVLAVVGALIGAVSFTGSCVAWAKLQGVLKKAYRLPAQNAVNV
                     VLALVSVGLGAAMVVLAPARPELIYGFFVVALVLGLIVTLPIGGADMPVVISLFNAFT
                     GLAVGFEGYVLGNPALIIAGIVVGAAGTLLTQLMAKAMNRPISNILFTPMTGGASSGE
                     AITGTMKEVGPIDAAAMMRYASKVIIIPGYGMAVAHAQHKVWEMTSILEEAGVEVKFA
                     IHPVAGRMPGHMNVLLAEAGVPYDKIFDLEEINGEFAQADVALVIGANDVVNPTARTD
                     KSSPIYGMPILNADLAQNVIVIKRGKGTGYSGVENALFYTDNCRMLYGDAQPMAGEII
                     QHLKAMG"
     gene            complement(2561..>2877)
                     /locus_tag="PKI23_11465"
     CDS             complement(2561..>2877)
                     /locus_tag="PKI23_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNN37593.1"
                     /translation="RRCQTDSGRRMMDGMLALYIFMLAAFTGYEIIAKVPVILHTPLM
                     SGSNFIHGVVVVGAMIVLGTADTLVQQAIGFFAVALGAANAAGGYVVTERMLGMFKKK
                     ED"
BASE COUNT          534 a          881 c          941 g          521 t
ORIGIN      
        1 gcgtttcacc gccctggccg tgcgcatggt ggtggctgtg cgtatgatcg gtgcattggt
       61 gctcgccggg gtgcgaatcg gcgtcaccgg tgccgtggtg cgcgtgtcgc cacagggcaa
      121 atttttccag tgcgaagaag ccgaggatgc cgccgagcag caaggggaag acctcgtgcg
      181 gcgtcagccc catctcgagc gcctcgggca gcagatggag catcgccatc gccagtagca
      241 ggccggtgga gaaactgacc gtgaatgcca gccacttgcg cggcaggccg aacatgatca
      301 gtgccgccgc agcaatgctc aggacgccgc cgagcaggca ggcgaggatg atctgcggca
      361 gcaggggcag ggaagacagc agggacatgg ggaggtagtt cgcgtcggac gcaatcgttc
      421 aggcaagcct gttttttgaa ggcgctattc aatcacagca gccacttaaa cgccacattg
      481 ttgcatctga gttgcatttg ctgcgctggc ccggggccgg gcgtaagcgc cgccgggaac
      541 cttgcggaca agcgaaggtc ttcagcttgt cctgaatgga gcttgatcat gaactgtgtt
      601 tggaagcggg cgctagccgc cctgttgatt ctggcatcac cactggccgt agcggacgac
      661 cgtcccgtga cgaccgtccc gaatgttgat ctggagcgct atctggggcg ctggtacgag
      721 atcggcagtt tcccgatgtt cttccagcgt aactgcatcg gggagacgac cgccgactat
      781 gcgctgcagg agggtgggag catctccgtc cgcaaccgct gccgaagcga ggacggcttc
      841 atcgaggccg aaggccgggc gaccccggtc gccggctccg ggaatgcccg gctgaaggtt
      901 tccttcttct ggccgttctc atccgactac tggatcatcg ggctggacga tgcttaccgc
      961 tgggcggttg taggtaatcc caaccgcaag tatctctgga tactttcccg cacgccggtg
     1021 cttgaccctg ctgcactcga acaagcccgc atgattgccg cagcacaggg tttcgacctt
     1081 gcccagttgc gcatgacaag gcagcttgcc gagtagggta ccggagaggg ggctgacgaa
     1141 aaagggggcg aaggccccct tttcgttgcc cgtagcacgc agattcagcc catcgccttg
     1201 aggtgctgga tgatctcgcc cgccatcggt tgcgcgtcgc cgtagagcat gcggcaattg
     1261 tcggtgtaga agagcgcgtt ttcgacgccc gaatagccgg tgcccttgcc gcgtttgatg
     1321 acgatgacgt tttgcgccag atcggcgttc aggatcggca tgccgtagat cgggctcgat
     1381 ttgtcggtac gcgccgtcgg attcacgaca tcgttggcac cgatgaccag cgccacgtca
     1441 gcctgcgcga actcgccgtt gatttcctcg agatcgaaga tcttgtcata gggcacgccg
     1501 gcttccgcca gcagcacgtt catgtggccc ggcatgcggc cggccaccgg gtggatggcg
     1561 aacttcactt cgacgccggc ctcttccagg atcgaggtca tttcccagac cttgtgctgg
     1621 gcgtgcgcca cggccatgcc gtagccgggg atgatgatca ccttgctcgc atagcgcatc
     1681 atcgctgcgg catcgatggg gccgacttcc ttcatcgtgc cggtgatcgc ttcgccactg
     1741 gaggcaccgc cggtcatcgg cgtgaacagg atgttcgaga ttggccggtt catcgccttc
     1801 gccatcagct gcgtcagcag cgtgccggcg gcaccgacga cgatgccggc gatgatcagc
     1861 gccggattgc ccagcacgta gccctcgaag cccaccgcga ggccggtgaa ggcgttgaac
     1921 agcgagatca ccaccggcat gtcggcgccg ccgatcggca aggtcacgat caggccgagt
     1981 accagcgcga caacgaagaa gccgtagatc agctcggggc gggccggtgc aagcacgacc
     2041 atggccgcgc ccagaccgac cgacaccagg gcgagcacga cgtttacggc attctgtgcc
     2101 ggcaggcgat aggccttttt cagtacgccc tgcagcttgg cccaggcgac acaggagccg
     2161 gtgaaggaga cggcaccgat cagcgcaccg acgacggcga gaacggtcgt tgccatcccg
     2221 tgcgtatcac ccctggcgaa ctcgacggca gcgatggccg cggccgcgcc cccgcccatg
     2281 ccgttgtaga tggcgaccat ctggggcatg tcggtcatct tcacctgctt gccggagatc
     2341 cacgccgcgg cgccgccgag cgccagcgcg agcgccatca ggccgaggtt ctgcaggccg
     2401 ggaatgaaga atacggccgc gatcgcgagc agcatgccgt agccggcgat gacgatgccg
     2461 cgttgcgcac tggcggggga actcatgccc ttgaggccga agatgaagag cagtgcgccg
     2521 gcgaaccagg caccttgaac atagatgggc atctgcatgt tcaatcctcc ttcttcttga
     2581 acatgccgag catgcgttcc gtgacgacgt agccgccggc ggcattggct gccccaagcg
     2641 cgacggcaaa gaagccgatc gcctgctgga cgagcgtatc ggccgtaccg agcacgatca
     2701 tggcgccgac cactaccacc ccgtggatga agttcgatcc cgacatcagg ggtgtgtgca
     2761 ggatgaccgg caccttggcg atgatctcgt agccggtgaa ggcggcgagc atgaagatgt
     2821 agagtgcgag catgccgtcc atcatgcgtc tcccagaatc tgtttgacac cggcgtg
//
LOCUS       DAORCQ010000167         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_32745_length_2791_cov_5.069444, whole genome shotgun sequence.
ACCESSION   DAORCQ010000167 DAORCQ010000000
VERSION     DAORCQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32745_length_2791_cov_5.069444"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKI23_11470"
     CDS             complement(<1..210)
                     /locus_tag="PKI23_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005742274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNN37594.1"
                     /translation="MKGQEALRRWTAEPPPREVQEALARLARAPDVVRVAAMPDVHLA
                     EDVCVGTVVATRATLIPAAVGGDIGC"
     gene            274..1215
                     /gene="aceE"
                     /locus_tag="PKI23_11475"
                     /pseudo
     CDS             274..1215
                     /gene="aceE"
                     /locus_tag="PKI23_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017136472.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
     gene            1257..2561
                     /locus_tag="PKI23_11480"
     CDS             1257..2561
                     /locus_tag="PKI23_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNN37595.1"
                     /translation="MNKIEVVVPELGGADHAEVIEIPVEAGVQIAAGATLVVVESEKA
                     AMEIPAPVAGQVMAIKVALGDEVRAGTLIALIESVQAAPANIEVEPPAAAIAEAPAPT
                     PPKPVEPAVQAPEPSPARTEPAAAAMAEGEKLLHAGPAVRKLARELGVPLEQVKPTGP
                     RQRITKEDLTGFIRQRVAAPPMTTPGLPLEPVFGPTLADAARFGAVRSEPLSRIQRVA
                     ARNLSRSWLTIPHVTHFDEADIGELEKFRRAERERAPADAAPLTILPFIIRAAVAALR
                     LHPRFNASLDSSGEALIIKEHYHLGIAVDTPQGLLVPVLRDAQDKGLLQLSAELRDLS
                     ERARQLRLKPDEMQGATFTITSLGGLGGIGFTPIINAPEVAILGVCRAQIKPHFDGIG
                     FVPSTRLPLSLSYDHRVLNGADAARFCNQLVANLEDLRRLLL"
BASE COUNT          480 a          907 c          921 g          483 t
ORIGIN      
        1 gcagccgatg tccccgccga ccgcggccgg gatcagcgtc gcgcgcgtcg ccaccaccgt
       61 cccgacgcac acgtcctccg cgaggtgtac gtccggcatc gccgccacgc ggaccacgtc
      121 cggcgcgcgg gccagccgcg cgagggcctc ctgcacctcg cgcggcggcg gctccgcggt
      181 ccagcgtctc agggcctcct gccccttcac gccagatcct ccggcaggtc ctcgcggacc
      241 acccggaaga gcagcccggg cgtccgcttg cggtggatcg ccgcggccac ctcctacagc
      301 agcaaccggt tgtcgatgat tccgttcttc atcttctatt cgatgttcgg cctgcagcgg
      361 gtgggggatc tggtgtgggc ggccggagac atccaggcgc gtggctttct gatcggcgcc
      421 acctccggcc gcaccaccct gaacggcgag gggttgcagc atcaggatgg ccacagccac
      481 ctgctggcgt cgaccctgcc caactgtgtc gcctacgatc ccgcctatgc ctatgaactg
      541 gcgctgatca tccgccacgg catcgagcag atgtatggcg aagggcgcaa ctgtttctat
      601 tacctcacgg tgctgaacga aaacttgcca cagccggcgc tgccggaggg tgtcgaggag
      661 ggggtgattc gcggcattta tctgctgaaa gagctgcaac cgacgcaaca gccggcgcgc
      721 ggagagatcc aactgctcgg cagtggtgcc atcctgcgcg aggtcgaggc ggcagcgacg
      781 attttggctg aagagtaccg gttggcggtc agactgttca gcgtgaccag cttcaatgaa
      841 ctgcggcgtg acggcttgga ggtgtcacgc tggaaccgtc tgcatcccga agccgagcca
      901 cggcagagcc atgtcgagca gctcctgggg cagtggcagg cgccagtggt ggccgcgacc
      961 gactatctca aaagcgtggc cgaccagatt cgtgagttcg tgccgggctg ttaccgcacg
     1021 ctgggcaccg atggtttcgg tcgcagcgac agccgtgcgc agttgcggcg cttcttcgag
     1081 gtcgaccgcc accatgtggt ggtgaccgca ctcaaggccc tcgccgacga gggcgtggtc
     1141 gcggcggccg aagtgagtgc agccatcgtt cgcttcggga tcgatgccga ccgaccgatc
     1201 gcgacccggc tgtaacccgt gccgccgttc gagcgcagca gagcaaggag atcaaggtga
     1261 acaagatcga ggtggtggtt ccggagctgg gtggcgcaga ccatgccgag gtgatcgaga
     1321 ttccggtcga agcaggcgtg cagattgcgg ccggcgccac gctggtcgtg gtcgaatcgg
     1381 aaaaggcggc aatggagatt ccggcgccgg tggcagggca ggtgatggcg atcaaggtgg
     1441 cgctgggaga tgaagtacgc gccggcaccc tgatcgcgct gatcgaatcg gtacaagccg
     1501 ctcccgccaa catcgaggtg gagccgcctg ccgcagccat tgccgaggcg ccagcgccaa
     1561 ccccgccgaa accggtcgag ccggccgtgc aggcgcctga gccttccccc gctcgcactg
     1621 aaccagcagc ggccgccatg gccgaaggtg aaaaactgct ccacgccgga cccgccgtgc
     1681 gcaaactggc gcgggagctg ggtgtgccac tggagcaggt caaaccgacc ggtcccaggc
     1741 agcgcatcac caaggaggat ctgaccggtt tcatccggca gcgggtggcg gcgccgccga
     1801 tgacgacgcc gggcctgccg ctggagccgg tattcggtcc gacgctggcc gatgcggccc
     1861 gctttggcgc ggtgcgcagc gagccactgt cgcgcatcca gcgggtggcg gcacgcaacc
     1921 tcagccgcag ttggctcacc attccccatg tcacccactt cgatgaggct gacatcggtg
     1981 aattggagaa atttcgtcgt gccgaacggg agcgtgcgcc agccgatgcc gcgccgctga
     2041 cgatattgcc gttcatcatc cgcgcagcgg tggcggcgtt gcgcctgcat ccgcggttca
     2101 acgcatcgct cgattcgagt ggcgaagcgc tgatcatcaa ggagcactac cacctcggca
     2161 tcgccgtcga cactccgcaa gggctgctgg tgccggtgct gcgtgatgcg caggacaagg
     2221 ggctgttgca gttgtccgca gagttgcgcg acctctccga gcgggcgcgg cagttgcgtc
     2281 tcaagccgga cgagatgcag ggtgccacct tcaccatcac cagtctgggt ggcctgggcg
     2341 gcatcggttt cacgccgatc atcaacgcgc cagaggtggc catcctcggt gtctgccgcg
     2401 cgcagatcaa gccccatttc gatggtattg gtttcgtgcc gagcacccgc ctgccgctgt
     2461 cactctccta tgaccaccgc gtgctcaatg gcgccgatgc tgcgcgtttc tgcaaccagt
     2521 tggtggccaa tctcgaggat ctgcgtcgcc tgttgctata gtgtgcgaac gggcatatcc
     2581 atcttgaccc gagatggata tgattcgcgc ggaatcgcgg ttggcccgcc gcgtttcgtt
     2641 atcgtcacga ctgggcctca tgggacacag gcatggatgc gtctgccgga ggcgatgctt
     2701 catgctggtt catctgcatc acctcatcca aggaaaccgt aaacatgtcc accgccaacg
     2761 cgaccgaaaa acccaccttt ctgaaactgc g
//
LOCUS       DAORCQ010000168         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_33218_length_2769_cov_3.847458, whole genome shotgun sequence.
ACCESSION   DAORCQ010000168 DAORCQ010000000
VERSION     DAORCQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33218_length_2769_cov_3.847458"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKI23_11485"
     CDS             complement(<1..353)
                     /locus_tag="PKI23_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37596.1"
                     /translation="MSNPDKDRMRKMWGLDEPNKPADPEVLGRRRYHEAAAKWLAKWS
                     YLLQTTFALIGFMVVLLPMFSKSWRAAIENTPVAERIFNDFSTLSGLAMVLFFILMVL
                     WGILNWRVKDYPGGW"
     gene            complement(356..2296)
                     /locus_tag="PKI23_11490"
     CDS             complement(356..2296)
                     /locus_tag="PKI23_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNN37597.1"
                     /translation="MPLPVVALLAAPEPANAETAIAVPARQTLSPALIIQQTDETLTW
                     VRPGQSAPETLSLTAARAQSAPLLLLVAQTEPPTAPSPSEAEPAKAQKKPFGFAWFIP
                     ELLRHKAIWRDVLLASLAIQLVGLATPLFTQVIIDKVIAHHTQSTLVVLGVALVVFML
                     FTSGMSWLRQYLILHTGARIDAVLGERVMRHLLRLPLPYFEQRPTGTLVARLHGVETL
                     REFVSGAAVSLLLDLPFLLIFLAVMFTYSWQLTLIALGILTLIVIASLIMVPTFRARL
                     DQQFLLGARNQSFLTEYLAGMATVKSLQLEPDVDKRYGNYLAQYLSASFATRQMGNTY
                     QVIASGLEQIMTLSILIVGAWMVMQNSGLTIGMLVAFQMFTGRLSQPVMRLVGLWQEF
                     QQATIAVKRLGDILDMPQEPFILTPQRASGGVGQIKIQDLSFRYSTQHPWLYHNLNLA
                     IPAGKLTVLTGPSGCGKSTLAKLMQGFYWPQEGQINLDGRDIRQLAANELRATFGVVP
                     QETVLFSGTVYDNLIMAHPHARFDDLIAACQAAEIHEVIEALPQGYQTEIGERGTGLS
                     GGQRQRIAIARALLKRPKVLIFDEAISNLDQQTAEQFAHTINRLKGKVTMIFITHQVP
                     KGLQVDEVVNMGRQAIQKNFGE"
     gene            complement(2401..2526)
                     /locus_tag="PKI23_11495"
     CDS             complement(2401..2526)
                     /locus_tag="PKI23_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37598.1"
                     /translation="MQGVHQKSTTKLEGEMATPAALWLLDNLAGFSVSRSMPRCC"
BASE COUNT          545 a          799 c          865 g          560 t
ORIGIN      
        1 tgccaccctc ctggataatc cttgacccgc cagttcagaa tcccccacag caccatcaga
       61 atgaaaaaca acaccatcgc caaaccgcta agggtcgaga aatcattgaa aatacgctca
      121 gcaactggtg tattctcaat cgctgcgcgc cagctcttcg agaacatcgg caacagcacg
      181 accatgaaac cgatcaaggc aaaggttgtc tgcaggaggt acgaccactt ggcaagccac
      241 ttcgcagccg cttcgtgata cctccggcgc cccaacacct cagggtctgc tggtttgttt
      301 ggttcatcta acccccacat tttgcgcata cgatctttgt ccgggttgct cacatctact
      361 ctccaaaatt cttctgaatg gcctgccgcc ccatgttcac cacctcatcc acctgcaagc
      421 ccttgggcac ctgatgggtg atgaagatca ttgtcacctt gcctttcagg cggttgatgg
      481 tgtgagcaaa ctgttcggcg gtttgttggt cgaggtttga aatcgcctcg tcgaagatca
      541 gcaccttggg gcgtttcagc agagcccggg cgatggcgat gcgttggcgt tgcccgcccg
      601 acagtcccgt gccgcgctcg ccgatttcgg tttgatagcc ttgcggcagg gcttcgatca
      661 cctcatggat ttcggcggct tggcaggcgg cgatcaggtc atcgaatcgg gcatgggggt
      721 gggccatgat caggttgtcg taaacggtgc cagagaacag caccgtctct tgcggcacca
      781 cgccaaaggt ggcgcgcagt tcgttggcag ccagttggcg gatgtcgcgc ccgtcgaggt
      841 tgatctgtcc ttcctgtggc cagtaaaacc cctgcatcag cttggccagc gtgctcttgc
      901 cacagccgga tggccccgtc agcacagtga gcttgccggc aggaatggcc aggttcaggt
      961 tgtggtagag ccaggggtgt tgggtggaat agcgaaagct caggtcctgg attttgatct
     1021 ggcccacccc gccgctggcc cgctgcggcg tgaggatgaa gggttcctgc ggcatgtcga
     1081 ggatgtcgcc cagccgcttg acggcaatgg ttgcctgctg aaactcctgc catagcccga
     1141 ccaaccgcat cacgggttga ctcaggcggc cggtgaacat ctggaaagcg accagcatgc
     1201 caatggtcag gccgctgttc tgcatgacca tccaggcacc cacgatcagg atggagagtg
     1261 tcatgatctg ctccagaccc gaggcaatca cctggtaggt attgcccatc tgccgggtgg
     1321 cgaaactggc cgagaggtac tgcgccagat agttgccata ccgtttgtct acgtcgggct
     1381 ccagttgcag cgatttcact gtggccatgc cggccaggta ctcggtgaga aaggactggt
     1441 tgcgggcacc gagcagaaac tgttgatcga ggcgggcgcg gaaggtgggc accatgatca
     1501 ggctggcgat gacgatcagt gtcaggatgc cgagcgcaat gagggtgagt tgccagctgt
     1561 aggtaaacat cacggccaaa aagatcagca gaaagggcag atcgagcagc aggctgactg
     1621 cggcgccgga gacgaactcg cgcagtgtct ctaccccgtg caggcgcgcc accagggtgc
     1681 cggtgggtcg ttgttcaaaa tagggcaacg gcaggcgcag caggtggcgc atcaccctct
     1741 cacccagcac cgcgtcgata cgcgcgccag tgtgcagaat caggtactgg cgcagccagc
     1801 tcatgccact ggtgaacagc atgaagacca ccagtgccac gcccagcacg accaaggtgc
     1861 tttgcgtgtg atgggcgatg accttgtcga tgatcacctg cgtaaaaagt ggcgttgcca
     1921 agccgaccaa ctgaatggcg aggctggcga gcagaacatc tcgccaaatg gctttatggc
     1981 gcagcaattc cgggatgaac caggcaaagc cgaagggctt tttctgtgcc ttggctggct
     2041 cggcttcgct cggcgatggc gcagtgggcg gttctgtctg tgccaccagc agcaacagtg
     2101 gtgcgctctg tgcgcgagcg gctgtcagcg acagcgtttc gggagccgat tggccgggac
     2161 gtacccaggt cagggtctcg tcggtctgtt ggatgatcag tgcagggctg agtgtctggc
     2221 gcgctggcac cgcgatggca gtttcggcat tggccggttc gggcgcggcg agcagcgcca
     2281 ccactggcag tggcaaggat ggccgctccg tcgccggcca ggccaccagg ccagccttga
     2341 agccaagctc ggccaatgcc tcgatgagtg tcggcagatc gatgggtggt ggaaagcgct
     2401 tcagcagcag cgtggcatcg aacggctgac ggaaaaaccg gccaggttgt ccagcagcca
     2461 gagtgcagcc ggggtggcca tttcgccttc gagtttggtg gtcgactttt gatgcacgcc
     2521 ctgcatctgc tgcctctcct tggcatccgt tcactgccgc tgagtatgga ttgaacaggc
     2581 tggaaaatgg aaatgtaatt ttcatgaatc ctgccgcccg gcatcgggtc taactcgccc
     2641 cagacgcatc ctgccgtagc agctccggta tgatccggcc ggtcaggtcc gtataatcgc
     2701 ggcttttgcg gcaggcacgc ccacggggtg tcgctgcgcc gcgctccttg aagtcgggat
     2761 ggtcatggc
//
LOCUS       DAORCQ010000169         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_36626_length_2624_cov_7.107824, whole genome shotgun sequence.
ACCESSION   DAORCQ010000169 DAORCQ010000000
VERSION     DAORCQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36626_length_2624_cov_7.107824"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKI23_11500"
     CDS             complement(<1..450)
                     /locus_tag="PKI23_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37599.1"
                     /translation="MEARIDVHLLTLSEPAEWRDACTASIEGAPIQLHVLPGIPGRIG
                     EARAAGYAQGTLPLVSFVDPDDLYEPSAFTQLADALDACPQAVMAYTDEALTDENGQD
                     IAVRRLAYSRWQHANSASHVHGLIVMRRSVVEAVLKETTDLNNFADWL"
     gene            complement(462..>2624)
                     /locus_tag="PKI23_11505"
     CDS             complement(462..>2624)
                     /locus_tag="PKI23_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNN37600.1"
                     /translation="RTAWGDADRAPLSSGHGLTSLSINKPTLFGGDEREGGVVGTIDV
                     LSGHAGQGRNDYLMSRLGSSIPAFRGVLSLVARKILFAANNPYIKPWAVRVRRFTAGW
                     FDAPWMEWNAEVRTWDEDEGREISVGMNPAHILVQCLTDPHWGMGYPQSTIGWSFWNA
                     AWALSSEGFGLNLIWTRQQPIESFIGQVIDHIGGILYTDPEQGTFELKLLRDDYWIDS
                     LPQLGPDEIVRLERFERAQWGELPNELTVVYTDWQTGGDATVTVENLAAIQLQGGVIN
                     QRRDYPGVNYGPLAARLALRDLRALGSPLARMSLTVARDTLERAPLPGDVFLLNWPRL
                     GVDQMVVRVTGIDTGTLGAAEWRIEAMEDVFGMSNTVLSPPPPHVEEPTIEPLPPALV
                     LAVEVPYWELARRLSRADLAYLTDTDTYLGALAATGGTGQLNWQLAAGTSGGDLTSVV
                     GEDYAPLLMLDAALPASEVDAIGVPVTAISQPERLAEGDYAYLVDASGAIAEAVTVLA
                     FDAANATIDLARGVLDTTPQAHALGTRLIGVGEWLASEGAERAPGESVFVGAIPRTST
                     DQGDAVLAANGQPMVLAGRQALPYPPGRIRLNGQTEPAVVAGDITVTWAHRDRTQQTA
                     YLVQQDEGDIGPELGVTYTLRIRNRNDVLAHTETGLLGTTFIWTAAVAALDAGALGDR
                     ITVEISAERDGLSSWQPQVRVMDRAGYGLRWGQYWGGV"
BASE COUNT          458 a          865 c          842 g          459 t
ORIGIN      
        1 cagccagtcg gcgaagttgt tgaggtcggt ggtttccttg agcacggctt cgacgacgga
       61 tcgacgcatc acgatcaggc cgtgcacgtg gctggcgctg ttcgcgtgct gccaacggct
      121 gtaggccagg cgccgcaccg cgatgtcctg gccgttttcg tcggtcagcg cttcgtcggt
      181 gtaggccatc acggcttgcg ggcaggcatc cagcgcgtcg gccagttgtg tgaaggcact
      241 gggttcgtac aaatcgtcgg gatcgacgaa ggacaccagg ggcaatgtgc cctgcgcata
      301 gccagcggca cgtgcctcgc cgatgcgacc gggaatcccc ggcaagacat gcaactggat
      361 cggtgcgcct tcgatgctgg cggtgcaggc atcccgccat tcggcgggct cgctcagggt
      421 gagcaggtga acatcgatgc gtgcttccat tggcgcttcc atcacacacc tccccaatac
      481 tgtccccagc gcaggccgta gcctgcgcga tccatgaccc gcacctgggg ctgccagctg
      541 ctcaaaccat cgcgttcggc actgatttcg actgtgatgc ggtcgcccag cgcacctgca
      601 tccagcgcgg ccactgctgc cgtccagata aaggtggtgc cgagtagtcc cgtttcggtg
      661 tgcgccagca cgtcattgcg attgcggata cgcagcgtgt aggtcacgcc cagttccggg
      721 ccgatgtcgc cctcgtcttg ctgcacgagg taggcggttt gctgcgtgcg gtcgcggtgg
      781 gcccacgtga cggtgatgtc accggccacc acggcaggct cggtctggcc gttgaggcgg
      841 atgcgaccgg gtggatacgg caaagcctgc cgaccggcca gcaccatcgg ctgtccattg
      901 gcggccaaca cagcatcgcc ctgatcggtc gatgtgcgag gaatcgcgcc cacgaacacc
      961 gactcgcccg gggcccgctc cgcaccttcc gacgctagcc attcgccgac gccgatcaac
     1021 cgagtcccca aagcatgagc ttggggtgtg gtatcgagta cgccgcgtgc gagatcgatg
     1081 gtcgcgttgg cggcatcgaa ggccaggacg gtaacggcct ctgcaatcgc cccactggca
     1141 tccaccagat aggcgtagtc gccctcggcc agtctctccg gctggctgat ggccgtcacc
     1201 ggcacgccga tggcatcgac ctcactggct ggcaaggcgg catcgagcat gagcagcggc
     1261 gcatagtcct cgcccacgac ggaagtgagg tctccgcctg aggtgccggc agccagttgc
     1321 caattcagct gcccggtgcc accggtagca gccagcgcac caagataggt gtccgtgtcg
     1381 gtcaggtagg ccagatccgc acgcgacaag cgccgggcca gttcccaata cggcacctcg
     1441 acggccagca ccaaggcggg cggcaaaggt tcgatggtcg gctcctcgac gtgcggtggc
     1501 gggggcgaca gcacggtgtt gctcatcccg aacacatctt ccatggcttc gatgcgccac
     1561 tcggccgcgc ccaaggtgcc ggtgtcgatg ccggtgacgc gcaccaccat ctgatccaca
     1621 cccaagcgcg gccagttcag caggaacacg tcgccgggca atggcgcacg ttccagcgtg
     1681 tcgcgtgcca cggtcagact catccgggcc agtggcgaac ccaaggcacg caggtcacgc
     1741 aaggccagcc gggcagccag cggcccgtag ttgacgcccg ggtagtcgcg gcgctgattg
     1801 atcacgccgc cttgcaactg gatggcggcc aggttctcga ccgtgacggt ggcatcacca
     1861 ccggtttgcc agtcggtgta gaccacggtc agttcgttgg gtagctcgcc ccactgggcg
     1921 cgctcgaagc gttccagccg cacgatttcg tcaggcccca actgcggcag gctgtcgatc
     1981 cagtagtcgt cgcgcagcag cttgagctca aacgtgcctt gctccgggtc ggtgtagagg
     2041 atgccgccaa tgtggtcgat gacctggccg atgaagctct cgatgggctg ctgccgtgtc
     2101 cagatcagat tgaggccgaa gccctcactc gacaaagccc atgccgcgtt ccagaaactc
     2161 cagccgatgg tgctctgcgg atagcccata ccccaatgcg gatcggtgag gcactgcacc
     2221 agaatgtgcg ccgggttcat accgacgctg atctcacggc cttcgtcctc atcccaggtg
     2281 cggacttcgg cattccattc catccacggc gcatcgaacc aacccgccgt gaagcgccgg
     2341 acgcgcactg cccacggttt gatgtagggg ttgttggccg cgaacaggat cttgcgtgcc
     2401 accaaggaca gcacgccccg aaatgccgga atggaactgc cgaggcgact catcaggtag
     2461 tcgttgcgtc cttgtccggc atgaccagaa agcacatcga tggtgccgac cacgccgcct
     2521 tcgcgctcgt cgccgccaaa cagggtgggc ttgttgatgg agaggctggt cagcccatgc
     2581 ccgctggaca gcggcgcgcg gtcggcatca ccccacgcgg tacg
//
LOCUS       DAORCQ010000170         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_42172_length_2424_cov_4.751372, whole genome shotgun sequence.
ACCESSION   DAORCQ010000170 DAORCQ010000000
VERSION     DAORCQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42172_length_2424_cov_4.751372"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKI23_11510"
     CDS             <1..488
                     /locus_tag="PKI23_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNN37601.1"
                     /translation="RLYNRIASYMTGQRVDDLTSGFRLVRARRFREFMHLLPNGFSYP
                     TTSTMAFFRAGYPVGYLPIHAAARVGKSHIRPLHDGLRFLLIIFKVGTLYSPLKLFAP
                     LAIAHFMLGAGYYLFTFLTQHRLSNMSVFMFTAAVTIFLIGLVSEQITQLLYQPRERD
                     G"
     gene            481..1707
                     /locus_tag="PKI23_11515"
     CDS             481..1707
                     /locus_tag="PKI23_11515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNN37602.1"
                     /translation="MADAVGRRPAILVLSSTFPRWPGDHEPPFVLELSRRLAERFDVW
                     LLAPHAPGARRQEQMAGIEVERFRYAPDRLEQLAYQGGILARLKQQPWRWLLVPLFIT
                     AQWWALHRLLRRQHFDAIHSHWLIPQTLIALLAGAGRKAPLLCTSHGGDLFGLRAAPL
                     PAIKAWVLKRCSAVTVVSRAMRDEVQRLLPGQMAEVVPMGTDLTGRFTPGSDSARRGD
                     TLLFVGRLVEKKGVNHLLDALAQLQRQGRQLQLWIVGQGPGLEPLQAQTERLGLAAWV
                     TFWGAVEHARLADFYRLATLAVTPSVVAEGGDQEGFGLVIVEAMGCGCPVVASALPAI
                     GDIVIDGETGLLVPPADPTALAHAIGQLLDQPEQARRLATTARQRVVERFDWQQVTER
                     YADLLLRMIERNERPA"
     gene            1691..2419
                     /locus_tag="PKI23_11520"
     CDS             1691..2419
                     /locus_tag="PKI23_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNN37603.1"
                     /translation="MNGPPEPVALGAGFAVASGRQAKALKIMRVLEEGLGRPLDGLRL
                     LDVGTGSGEIAHILGQRCELVSIDPADQRQHVDGYRYVRAGTALPFAQASFDVAISNH
                     VIEHLDDAPLHLQELSRVVRPGGLVYLATPNRWWPWEVHYRLPLLHYLPRTLFWTLLR
                     RMGRFHEPLHLLGSRSLQRLAFGAGFAVTDCTPMIIRQPRRYHLDVADWLAALLGFLP
                     HAFYQRLRWAVPTLIFLLKRAQRP"
BASE COUNT          345 a          822 c          793 g          464 t
ORIGIN      
        1 accggctcta caaccgcatt gccagctaca tgaccggcca gcgggtcgat gacctcacct
       61 cgggctttcg gctggtgcgt gcccgccgct tccgtgaatt catgcacctg ctgcccaacg
      121 gtttttccta ccccaccacc agcaccatgg cgttctttcg cgccggctat ccggtgggct
      181 acctgccgat tcatgccgcg gcccgggtgg gcaagagcca catccggccg ctgcacgatg
      241 gcctgcgctt tttgctgatc atcttcaagg tgggcaccct ctactcgccg ctgaagctct
      301 ttgcgccgct ggccatcgcc cacttcatgc tgggtgccgg ttactacctc ttcaccttcc
      361 tgacccagca ccgactgtcg aacatgtcgg tgttcatgtt caccgccgcc gtcaccattt
      421 ttctgatcgg cctcgtctcc gagcagatca cccaactgct ctaccagccg cgagagcgcg
      481 atggctgacg ccgttgggcg gcggccggcc attctggtgc tcagctccac ctttccccgt
      541 tggccgggcg accatgaacc ccctttcgtg ctggagctct cccggcgatt ggccgagcgc
      601 ttcgatgtct ggctgctggc ccctcatgcg ccgggtgcac ggcgacagga gcagatggcc
      661 ggcatcgagg tcgaacggtt tcgctatgcg cctgaccgcc tggagcagct ggcctaccag
      721 ggcggcattc tggcccgcct gaagcagcag ccgtggcgct ggctgctggt gccgctcttc
      781 atcaccgcgc agtggtgggc gctgcaccgc ctgctgcgtc ggcagcattt cgacgccatc
      841 cacagccact ggctgattcc acagacgctg atcgcactgc tggccggtgc cggccggaag
      901 gcgccgctgc tctgcaccag ccatggcgga gacctcttcg gcctgcgcgc cgcgccgctg
      961 cctgccatca aggcctgggt gctgaagcgc tgctccgccg tcaccgtcgt cagccgggcg
     1021 atgcgtgacg aagtgcagcg gctgctaccg gggcagatgg ccgaagtggt gccgatgggc
     1081 accgatctga ccgggcgctt caccccgggt tccgactcgg cacggcgcgg cgacacgctg
     1141 ctgttcgttg gccggctggt cgaaaaaaag ggggtgaacc acctgctcga tgcgctggcc
     1201 cagctgcaac ggcaggggcg gcagttgcag ctctggatcg tggggcaggg ccccggactc
     1261 gagccgctgc aagcccagac cgagcggctt gggcttgcag cgtgggtgac cttctggggt
     1321 gccgtcgagc atgccagact ggccgatttc taccgtctgg cgaccctggc cgtcaccccc
     1381 tcggtggtgg ccgaaggggg tgatcaggag ggcttcgggc tggtcatcgt cgaagcgatg
     1441 ggttgcggct gtccggtggt ggcctccgcg ctgccggcga ttggcgacat cgtcatcgat
     1501 ggcgaaaccg ggctgctggt gccgccggcc gacccgaccg cgctggcgca tgccatcggc
     1561 caattgctcg accagccgga acaggcacga cgactcgcca ccaccgcccg gcaacgggtc
     1621 gtcgaacggt tcgattggca gcaggtgacc gaacgctatg ccgatctgct gctgcggatg
     1681 atcgaacgca atgaacggcc cgcctgagcc ggtcgcgctg ggtgccggct ttgcggtggc
     1741 cagcggccgc caggccaagg cgctcaagat catgcgcgtg ctggaggagg ggctgggccg
     1801 gccgctcgat ggactgcggc tgctcgatgt cggcaccggc agtggcgaga tcgcccacat
     1861 tctggggcag cggtgcgaac tggtcagcat cgacccagcc gatcagcggc agcatgtcga
     1921 cggctatcgc tatgtccgtg ccggcaccgc gctgcccttt gcgcaggcca gcttcgacgt
     1981 ggcgatctca aaccatgtga tcgagcacct ggacgatgca ccactgcacc ttcaggagct
     2041 gtcgcgggtg gtgcgtccgg gtggcttggt ctacctggcc acgccgaacc gctggtggcc
     2101 ctgggaagtg cattaccgcc tgccgctgct gcactatctg ccgcgcacgc tgttctggac
     2161 gctgctgcgt cgcatgggtc gctttcatga accgctccat ctgctgggct cccgctcgtt
     2221 gcagcggctg gcgtttggcg ccggttttgc cgtgaccgac tgtacgccga tgatcatccg
     2281 ccagccaagg cgctaccacc tcgatgtcgc cgactggctg gccgcactgc tgggcttttt
     2341 gccacacgct ttctaccaac ggttgcgctg ggccgtgccg acgctgattt ttctgctgaa
     2401 gagagcgcag cggccatgag tgtg
//
LOCUS       DAORCQ010000171         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_43579_length_2380_cov_5.778495, whole genome shotgun sequence.
ACCESSION   DAORCQ010000171 DAORCQ010000000
VERSION     DAORCQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43579_length_2380_cov_5.778495"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="PKI23_11525"
     CDS             <1..1725
                     /locus_tag="PKI23_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37604.1"
                     /translation="PQPLPDIWTQSRNGIGGVGWYRFDFDWSGDEPLTQAIYLPRLCM
                     NVAVYLNGVLIGDGGRFDEPLARNWNRPLLFLLPRDALRSGHNHLHLRLHTLGYSQNS
                     LYPPFIGPERELRPDYERLHFLRITFNQSMTLLITATGLLMLGLWWRRRQDTMYGYFG
                     LSALLWALNSTNLYLVKVPLASRDWEILIHSVFPLFTALLLISLLRLLDLRPRRLEQG
                     LWLTLLLPILTLPLAFTDGLPQLLVIWQSAPLPATGAMTLLLTRVAWQRRTPESLLLA
                     LALWLTISLGLHDWLLHNRWLVWSRSILWVRPGDLHLLHYGAPLLFLVVGWIMTSRFV
                     DVLNRFEQLNRDLELRVASKHAALEANFARIEELNREQAMLAERQRIVADLHDGLGGQ
                     LVTALRLVESKRIDATGMAELLRECLDEMRMVMNSITPQEHDLDGVLATLRHRFASRL
                     EQAGIELFWQLDSTLPDRLLSARQALQMQRIVQECFTNILKHAGASQVHVHSSEVDGV
                     RQLCIADNGRGMAPQADGTGHGIANMRRRMATLGGSIDIDSSAAGTRILLRLPPVGKG
                     WRTLPEQP"
     gene            1736..2377
                     /locus_tag="PKI23_11530"
     CDS             1736..2377
                     /locus_tag="PKI23_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNN37605.1"
                     /translation="MKNGLIVEDLAENRLWLSQTLQQSFADMAIHCTTSLAEARHWLA
                     TSPPPDMALIDLGLPDGSGIELIELLNQRAPATLCVVATIYDDDNHLFAALRAGAQGY
                     ILKDQGRDEAARLLQGIAAGQPPLSSSIARRILGSFKAEPPQPLPEAQPTLTPREHEV
                     LRLISKGMTLTETARLLQLSRHTVDGYVKEIYRKLNVSSRAEAALTAHNFGLV"
BASE COUNT          410 a          832 c          732 g          406 t
ORIGIN      
        1 ccgcagccac tgcccgacat ctggacccag agccgcaatg gcatcggcgg ggtcggctgg
       61 taccgcttcg atttcgactg gtcgggcgat gaacctctga cccaggccat ctacctgcca
      121 cggctctgca tgaacgtggc ggtctacctc aacggcgttc tgatcggcga tggcggccgc
      181 ttcgatgagc cgctggcgcg caactggaac cgtccgctgc tgtttttgct gccacgggat
      241 gcactgcgct ccggccacaa ccacctccat ctgcggctgc acaccctcgg ctactcgcag
      301 aacagcctct atccaccctt catcggaccg gagcgcgagc tgcgccccga ctacgagcgg
      361 ctgcactttc tgcgcatcac cttcaaccag agcatgacgc tgctgatcac ggcgaccggg
      421 ctgctgatgc tgggcctgtg gtggcgacgc cggcaggaca ccatgtatgg ctacttcggc
      481 ctgtcggcgc tgctctgggc actcaactcg accaacctct atctggtgaa ggtgccgctc
      541 gccagccgcg actgggagat tctgatccat tcggtcttcc cgctctttac cgccctgctg
      601 ctgatctcgc tgctgcgcct gctcgacctg cgcccgcgcc ggctggagca ggggctgtgg
      661 ctgaccctgc tgctgccgat cctgacgctg ccgctggcct tcaccgacgg attgccacaa
      721 ctgctggtca tctggcagag cgcaccgctg cccgccaccg gcgcgatgac gctgctgctg
      781 acccgggtcg cctggcagcg gcgcacgccg gagagcctgc tgctggcact ggcgctgtgg
      841 ctgaccatca gcctcggatt gcatgactgg ctgctgcaca accgctggct ggtctggagc
      901 cgatcgatcc tctgggtccg tccgggcgac ctgcatctgc tgcactatgg cgcgccactg
      961 ctgtttctgg tggtgggctg gatcatgacc agccgcttcg tcgatgtgct caaccgcttc
     1021 gaacagctca accgcgatct ggagctgcgg gtcgccagca aacatgccgc gctggaggcc
     1081 aacttcgccc gcatcgagga gctgaaccgt gagcaggcga tgctggccga acgacaacgc
     1141 atcgtcgccg acctgcacga tggtctgggt ggacaactgg tcaccgcgct gcgactggtg
     1201 gagtcgaaac gcatcgatgc caccggcatg gcggaactgc tgcgcgaatg tctcgatgag
     1261 atgcgcatgg tgatgaactc catcaccccg caggagcatg acctcgacgg cgtgctggcg
     1321 acactgcgcc accgctttgc cagccggctg gagcaggccg gcatcgagct gttctggcaa
     1381 ctcgacagca cgctgcccga ccgtctgctg agcgcccggc aggcgttgca gatgcagcgc
     1441 atcgtgcagg agtgcttcac caacatcctc aaacatgccg gcgccagcca ggttcatgtt
     1501 cacagcagcg aggtcgatgg agtgcgccag ctctgcatcg ccgacaacgg ccgcggcatg
     1561 gcaccgcagg cggatggcac tggccacggc atcgccaaca tgcgcagacg catggccacg
     1621 ctggggggta gcatcgacat cgacagctcg gcagcgggca cccggattct gctgcggcta
     1681 ccccccgtgg gcaaggggtg gcgcaccctg ccggaacaac cataatggcc cgatgatgaa
     1741 aaacggattg atcgtcgagg atctggccga aaacagactc tggctgagcc agacgctgca
     1801 acagagcttt gccgacatgg ccatccattg caccaccagc ctcgccgagg cacgccactg
     1861 gctggccacc tccccaccgc ccgacatggc gctgatcgat ctgggcctgc ccgatggcag
     1921 cggcatcgag ctgatcgaac tgctcaatca gcgcgcgccg gcgaccctct gcgtcgtcgc
     1981 caccatctat gacgacgaca accacctttt tgccgccctg cgcgccggcg cccaaggcta
     2041 catcctcaag gatcagggcc gcgacgaggc agcccggctg ctgcagggca tcgccgccgg
     2101 gcagccaccg ctgtcgtcga gcatcgcccg gcggattctt ggttcgttca aggcagagcc
     2161 gccccagcca ctgccggagg cgcagccgac gctgaccccg cgcgagcatg aggtgctgcg
     2221 gctgatcagc aagggcatga ccctgaccga aaccgcccgg ctgctgcaac tgagccgcca
     2281 caccgtcgat ggctacgtca aggagatcta ccgcaagctc aatgtctcct cccgcgccga
     2341 ggcggcactg accgcccaca acttcgggct ggtgtaggcc
//
LOCUS       DAORCQ010000172         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_43807_length_2372_cov_5.567544, whole genome shotgun sequence.
ACCESSION   DAORCQ010000172 DAORCQ010000000
VERSION     DAORCQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43807_length_2372_cov_5.567544"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKI23_11535"
     CDS             complement(<1..335)
                     /locus_tag="PKI23_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNN37606.1"
                     /translation="MDDVQRHGATEMTAPMLEAVKLSVRFGGLLAVNNVSFEVWPNEV
                     FSLIGPNGAGKTTIFNLISSLYRPTSGEVLFEGEVISRMPPHVVARHGIARTFQNIEL
                     FEHATVLQNL"
     gene            complement(645..1721)
                     /locus_tag="PKI23_11540"
     CDS             complement(645..1721)
                     /locus_tag="PKI23_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNN37607.1"
                     /translation="MHFQFKTSYAQDIALFKHNGQRFWYGLLLLGLIAAPWLLNEYAL
                     SQFAFIGIYAIICLGLMLLSGFTGLVSLGHAGFLAMGAYTEAWLQARGWPFALSLPLS
                     ALVAGLTGIVVGLPALRIKGMYLAIATLAFGFIIEEAVTRAEHITGGNAGVQVGKLMI
                     AGIDFDDGVNFYFLVTSIAMLCVFGVLNLLRSPTGRAFVAIRDSEVSAQSMGINLAYY
                     KTVAFALSAALAGIAGALYAHKIRIITPDQFGFLQSIELLMMVVIGGLGSVQGAIFGP
                     AFWFIVQQVIVVGKDWLPPAIGQQTGLQPTVFGIILIAVVLFEPMGLYGRWLKVRTFL
                     QIFPFYRKGMFKRQKSYMKSERMK"
     gene            complement(1732..>2372)
                     /locus_tag="PKI23_11545"
     CDS             complement(1732..>2372)
                     /locus_tag="PKI23_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNN37608.1"
                     /translation="RPLLGQPAFTVVMVTIGLGYLGRGVVTMIPYWGTETHTLPVPYK
                     DEVIWFGETGKGLVVSVEHIAIIVATVLLVALLYAFFRYTKLGIAMQATSQNQLAAFY
                     MGIPVRRVNMLIWGISSAICGAAALLLAPITFVHANMGFVGLKAFPAAVVGGFGSIPG
                     ALVGGLIVGIVESLAGFYLPEGFKDIAAYIVVLIMLVVKPNGLFGENTTKKV"
BASE COUNT          472 a          725 c          805 g          370 t
ORIGIN      
        1 aggttctgca gcaccgtggc gtgttcgaac agctcgatgt tctggaaggt gcgggcgatg
       61 ccgtgccgcg ccaccacgtg cggaggcatg cgagagatca cctcgccctc gaagaggacc
      121 tcgccggaag tcggccgata gagcgaagag atcaggttga agatggtggt cttgccggcg
      181 ccattggggc cgatcagcga gaacacctcg ttgggccaga cctcgaagga gacgttgttc
      241 acggccagca ggccgccgaa gcggaccgag agcttgacgg cctcgagcat cggggcggtc
      301 atttcagttg ctccatgacg ctgtacgtcg tccatggcca atgagacatg gtgtcgtggc
      361 gcacgccatg gatggcgggt acccgattcc ctccgtgtcc cccggaacgg gcgttgcccg
      421 ttcctcctcc tttacctgcg ggaatcgggt acccgccatc catggccgga tacgctcctg
      481 ctttgcgacg ttcaaacccc tgcgatgtat ccggatcggg tcggtggggg actctgctcc
      541 gcgcaagtaa aggaggagga ggccgcaggc cgacggggga catggagcgg agcagagtcc
      601 cccatcgacc cgatcccgca ccaacctcga ttccgcgacg ggtttcactt catccgctcc
      661 gacttcatgt agctcttctg ccgcttgaac atgcccttgc ggtagaaggg gaagatctgc
      721 aggaaggtgc gcaccttcag ccagcggccg tagaggccca tgggctcgaa cagcaccacg
      781 gcgatcagga tgatgccgaa cacggtgggc tgcaggccgg tctgctggcc gatggccggc
      841 ggcagccagt ccttgccgac cacgatcact tgctgcacga tgaaccagaa ggccgggccg
      901 aagatcgcgc cctgcaccga gccgaggccg cctatgacca ccatcatcaa cagctcgatc
      961 gactgcagga aaccgaactg gtcgggcgtg atgatgcgga tcttgtgcgc gtagagcgca
     1021 ccggcaatgc cggccagcgc cgccgagagg gcgaaggcga cggtcttgta gtaggcgagg
     1081 ttgatgccca tgctctgcgc cgagacctcg gaatcgcgga tggcgacgaa ggcgcgcccg
     1141 gtcgggctgc gcagcaggtt gagcacgccg aatacgcaaa gcatggcgat ggacgtgacg
     1201 aggaagtaga aattcacccc gtcgtcgaag tcgatgccgg cgatcatcag cttgccgacc
     1261 tggaccccgg cattgccgcc agtgatgtgc tctgcgcgcg tcacggcttc ctcgatgatg
     1321 aaaccgaagg ccagcgtggc aattgcgagg tacatcccct tgatgcgcag cgccggcagg
     1381 ccgacgacga tgccggtgag gcctgccacc agggcagaca gcggcagcga aagggcaaag
     1441 ggccagccgc gcgcctgcag ccaggcctcg gtataggcgc ccatggcaag aaagccggca
     1501 tggccgagcg agaccagtcc ggtgaagccc gagagcagca tcaggccgag gcagatgatg
     1561 gcgtagatgc cgatgaaggc gaactgcgac agcgcatatt cgttgagcag ccagggcgcg
     1621 gcgatcaggc cgagcagcag caggccgtac cagaagcgct ggccgttgtg cttgaacagc
     1681 gcaatgtcct gggcatacga ggtcttgaat tgaaagtgca tgcgccggcg ctcagacttt
     1741 cttggtggtg ttctcgccga acaggccgtt cggcttcacc accagcatga tcagcaccac
     1801 gatgtaggcg gcgatgtcct tgaagccttc cggcaggtag aagcccgcca gcgactcgac
     1861 gatgccgacg atgaggccgc cgaccagtgc ccccgggatg ctgccgaagc cccccaccac
     1921 cgcggcgggg aaggccttga ggccgacgaa gcccatgttg gcgtgcacga aggtgatcgg
     1981 ggcgagcagc agcgcggcgg caccgcaaat cgccgacgag atgccccaga tcagcatgtt
     2041 cacccgccgt accgggatgc ccatgtaaaa ggcggcgagc tggttctgcg acgtcgcctg
     2101 catggcgatg ccgagcttgg tgtagcggaa gaaggcgtac agcagcgcca ccagcagcac
     2161 cgtggcgacg atgatggcga tgtgctcgac ggaaacgacg agacccttgc cggtctcgcc
     2221 gaaccagatc acttcgtcct tgtagggcac gggcagggtg tgggtctcgg tgccccagta
     2281 gggaatcatg gtcacgacgc cgcggccgag gtagccgagg ccgatggtca ccatcaccac
     2341 ggtgaaggcc ggctggccga gcagggggcg ca
//
LOCUS       DAORCQ010000173         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_49556_length_2215_cov_4.069907, whole genome shotgun sequence.
ACCESSION   DAORCQ010000173 DAORCQ010000000
VERSION     DAORCQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49556_length_2215_cov_4.069907"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKI23_11550"
     CDS             complement(<1..586)
                     /locus_tag="PKI23_11550"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNN37609.1"
                     /translation="MQARIDQLLELLDLEPLDTHLFRGVSPAGERNRVFGGQVLGQAL
                     VAAGRTVDERLLHSMHAYFLRPGDPKRPILYEVERSRDGGSFTSRRVVAIQQGKPIFN
                     LSASFQRAEPGLDHQAAMPDTASPELFITEEEQLALLYQQDPQNYRHPDYFQWPIEFR
                     HVDHSNSRRHEVHPPQHRVWMRAVAPLPDALILHQ"
     gene            794..>2215
                     /locus_tag="PKI23_11555"
     CDS             794..>2215
                     /locus_tag="PKI23_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNN37610.1"
                     /translation="MVRLPVIVGFGGINPAGRSSFHHGYRRLVLDVLDETSRNETLVD
                     LAMMMNLARFEEGHWRDHDGTPLTPSQLAQRHRAQIERHTLIRRIEQSHFDVDAVPFN
                     RKLTLQPLAGPLEFALPVRQLPDPLPPGWQVTPIDQRQVRVTLTDGLDLFLPDQKKAL
                     VSSAGQLPSGFDPGALYPSRNHPRALQMALYAASDALGSLGIDWSSLLKRVAPDQIAV
                     YASSSMGQLDDNGTGGMYKAPYIGKRISSKQLPLGFAEMPADFVNAYVLGSVGATGGA
                     VGACATFLYNLRSGVDDIRSGRRRVVLVGATEAVINPEAMEGYAAMGALAEDAELINL
                     DQKLGLTEPDHRRACRPFNYNCGFTMGESAQFLVLFDDALALEMGAQIHGAVPMVSVH
                     ADGFKKSIAGPGVGNYLTVGKAMATARDLLGEAKLRHGSYMHAHGTGTPQNRVTESRI
                     FNEFAKLHGIEQWPVAAIKCYLGH"
BASE COUNT          392 a          731 c          716 g          376 t
ORIGIN      
        1 attgatgcag aatcagcgca tcgggcagcg gcgccaccgc ccgcatccac acccggtgtt
       61 gtggtggatg gacctcatgg cgccggctgt tgctgtggtc gacatggcgg aactcgatcg
      121 gccactgaaa atagtcggga tggcggtagt tctgcggatc ctgctggtag agcagtgcca
      181 actgctcctc ctcggtgatg aacagctctg gtgacgcggt gtccggcatc gccgcctgat
      241 ggtcgaggcc gggctcggcg cgctggaacg aggccgacag attgaagatc ggcttgccct
      301 gctggatcgc caccacccgg cgcgaggtga agctgccacc atcgcggctg cgctccacct
      361 cgtacagaat cggccgcttc gggtcgcccg gccgcagaaa gtaggcatgc atcgagtgca
      421 gcagccgctc atcgaccgtg cggcccgccg ccaccagcgc ctgccccagc acctggccgc
      481 caaacacccg gttgcgctcg ccggccgggc tgacgccgcg aaacaggtgg gtgtcgagcg
      541 gctccagatc gagcagttcc agcagttggt cgatgcgcgc ttgcatggcg ggtgcgtact
      601 ccggcgtgaa caaggtggtg cgggcggatg gccctgaatg gtaactgatc ggccgcccgc
      661 atggcgaaac cggcaaattt cagcaaaatc cattcgttgg acgcattgtt gcaggcaggc
      721 ttggaaggtt tgggcctact caatacaatg gcccgtcatc cagaggtcat cattgcacgc
      781 aggagaaggg cgcttggtca gattaccggt gatcgtcggg ttcggtggca tcaatccggc
      841 cggtcgcagc tccttccacc atggctaccg ccggctggtg ctcgatgtgc tcgatgagac
      901 cagtcgcaac gaaacactgg tcgacttggc gatgatgatg aatctggccc gcttcgagga
      961 gggccactgg cgcgaccacg acggcacgcc gctgacgccc tcgcaactgg cgcagcgcca
     1021 ccgcgcgcag atcgagcgcc acaccctgat ccgccgcatc gagcagagcc acttcgatgt
     1081 cgacgcggtg ccgttcaacc gcaagctgac actgcaaccg ctcgctggac cgctcgaatt
     1141 cgcgctgcca gtgcggcagt tgcccgaccc gctgccgccc ggctggcagg tgacgccgat
     1201 cgatcaacgt caggtcaggg tcaccctcac cgatgggctc gatctgtttc tgcccgacca
     1261 gaaaaaggcg ctggtgtcga gcgccggcca gttgccgagc ggcttcgacc ccggcgcgct
     1321 ctatccatcg cgcaaccacc cgcgcgcgct gcagatggcg ctctatgcgg cttctgacgc
     1381 gctcggttcg ctcggcatcg actggagcag cctgctgaag cgggtggcac ccgaccagat
     1441 cgctgtctat gccagcagct cgatgggcca gctcgacgac aacggcaccg gcggcatgta
     1501 caaggcgccc tacatcggca agcgcatcag ctccaagcag ttgccgctcg gctttgccga
     1561 gatgccggcc gacttcgtca atgcctatgt gctcggcagc gtcggtgcca ccggcggcgc
     1621 ggtcggcgcc tgtgccacct tcctctataa cctgcgcagc ggcgtcgacg acatccgctc
     1681 cgggcggcga cgggtggtac tggtcggtgc caccgaggcg gtgatcaacc ccgaagcgat
     1741 ggagggctac gccgccatgg gcgcgctggc cgaagatgcc gagctgatca acctcgatca
     1801 gaagctgggt ctgaccgagc cagaccaccg ccgtgcctgc cgcccgttca actacaactg
     1861 cggcttcacg atgggcgaat cggcgcagtt cctggtgctg ttcgacgatg cgctggcgct
     1921 ggagatgggg gcgcagatcc atggcgccgt gccgatggtc agcgtccatg ccgacggctt
     1981 caagaagtcg atcgccggtc ccggtgtcgg caactatctg acggtcggca aggcgatggc
     2041 cactgcccgc gacctgctcg gcgaggcaaa attgcggcat ggcagctaca tgcatgccca
     2101 cggcaccggc acgccgcaga accgcgtcac cgagtcgcgc attttcaacg aattcgccaa
     2161 actgcatggc atcgagcagt ggccggtcgc ggcgatcaaa tgctacctcg gccac
//
LOCUS       DAORCQ010000174         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_60685_length_1986_cov_5.453651, whole genome shotgun sequence.
ACCESSION   DAORCQ010000174 DAORCQ010000000
VERSION     DAORCQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60685_length_1986_cov_5.453651"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKI23_11560"
     CDS             complement(<1..262)
                     /locus_tag="PKI23_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010445678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNN37611.1"
                     /translation="MPTLNWIGKQAVVKHHKEVPFRLLEPLPELSLPSPAGRGAGGEG
                     DHGDNLIVQGDNLHALKALLPRYAGQVKCIYIDPPYNTGNEGW"
     gene            433..1089
                     /gene="ccmA"
                     /locus_tag="PKI23_11565"
     CDS             433..1089
                     /gene="ccmA"
                     /locus_tag="PKI23_11565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010211794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNN37612.1"
                     /translation="MQSAEQSIDAVPLLQAVGLGCERDDRTLFRRLDFAVQGGELLEV
                     RGRNGSGKTTLLRMVAGLGEPDEGELRWRGRPLRRMRAELGSELCYLGHLPAIKETLT
                     ARENLRFAMQLKGCCASERIDAALAESGLRGFEDLPCRQLSAGQRRRVALALLRLLPA
                     RLWILDEPFTALDRQAVAQLEQWIEAHRAQGGAVLLTTHQPLGLGSAWRTLTLGESVA
                     "
     gene            1110..1778
                     /gene="ccmB"
                     /locus_tag="PKI23_11570"
     CDS             1110..1778
                     /gene="ccmB"
                     /locus_tag="PKI23_11570"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNN37613.1"
                     /translation="MHRIAAGLLRRELLLAWRRPGESLNPLLFYLLVGSLFPLGIGPD
                     AAFLARIAAGVIWIAALLALLLSFDNLFRADHDDGTLEQFLLSPCPLYFLVLVKLAAH
                     ACIVGLPLLLATPLLGLMLALPAPAIGTLLCTLLLGVPTLVMLGGVGAALTLGLRRGG
                     LLLSLLVLPLYVPVLIFGASAVQATQSGLPVTGHLAFIGALLAATLVTTPFAIAAALR
                     ISGG"
     gene            1782..>1986
                     /locus_tag="PKI23_11575"
     CDS             1782..>1986
                     /locus_tag="PKI23_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37614.1"
                     /translation="MDFTATFTRWFHRMASPRWFYRFARRWQPWFGWSSLLLLLVGAV
                     WGLAFAPPDYLQGNSFRIIYIHVP"
BASE COUNT          235 a          668 c          712 g          371 t
ORIGIN      
        1 cccagccctc gttgccggtg ttgtagggcg ggtcgatgta gatgcacttc acctggccgg
       61 cgtagcgcgg cagcagcgcc ttgagcgcgt gcaggttgtc gccctggacg atcaggttgt
      121 cgccgtggtc accctcaccc ccggcccctc tcccggcggg agaggggagg gacagttccg
      181 gcagcggctc cagcaggcgg aacggcacct ccttgtggtg tttgacgacg gcttgcttgc
      241 cgatccagtt gagtgtgggc atcgatggct ctgcatcctg tgcgctcggc attgaccgcc
      301 ggcgggcggc gcagagcata ccgcttttcg agggtcgatg cgccgcatcc gccgggcgca
      361 ccatttttat gcgatccggc cagcgggagt cgctataatc agccgccgca atccgggtcg
      421 gagcccattc tggtgcagtc agccgaacag tcgatcgatg ccgtgccgct gctgcaagcc
      481 gtggggcttg gctgtgagcg cgatgaccgc acgctgttcc ggcggctcga ctttgccgtg
      541 cagggcggcg agctgctgga ggtgcgaggc cgcaatggca gcggcaagac gacgctgctg
      601 cggatggtgg ccggcctcgg cgagccggac gagggcgagc tgcgctggcg cggccggccg
      661 ctgcggcgga tgcgcgccga gctcggcagc gagctctgct acctcggcca tctgccggcg
      721 atcaaggaga cgctgaccgc ccgcgagaac ctgcgcttcg ccatgcagtt gaagggctgc
      781 tgcgccagtg aacggatcga cgcggcactg gccgagtcgg ggctgcgcgg cttcgaggat
      841 ctgccatgcc gtcagctctc ggccgggcag cggcggcggg tggcgctggc gctgctgcgg
      901 ctgctgccgg cgcggctgtg gattctcgac gagcccttca ccgcgctcga ccgtcaggcg
      961 gtcgcgcaac tggaacagtg gatcgaggca caccgggccc agggtggcgc ggtgctgctg
     1021 accacccatc agccgttggg ccttggcagt gcgtggcgga cgctgacgct cggggagtcc
     1081 gttgcttgag cagtgcaacc ggcgtggcac tgcaccggat cgcggccgga ctgctgcggc
     1141 gcgagctgct gctggcgtgg cgccggcccg gcgagagtct caatccgctg ctgttctatc
     1201 tgctggtcgg cagcctgttc ccgctcggca tcggtccgga cgcggccttt ctggcccgca
     1261 tcgccgccgg cgtgatctgg atcgccgcgc tgctggcgct gctgctgtcg ttcgacaacc
     1321 tgtttcgcgc cgaccatgac gacggcacgc tggagcagtt tctgctgagc ccctgcccgc
     1381 tctattttct ggtgctggtc aagctggcgg cccatgcctg catcgtcggg ctgccgctgc
     1441 tgctggcgac cccgctgctg gggctgatgc tggcattgcc ggccccagca atcggcacac
     1501 tgctctgcac gctgctgctg ggcgtgccga cgctggtcat gctgggcggc gtcggcgcgg
     1561 cgctgacgct ggggctgcgc cgcggcggac tgctgctgtc gctgctggtg ctgccgctct
     1621 atgtgccggt gctgatcttt ggcgccagcg cggtgcaggc gacccagagc ggtctgccgg
     1681 tcaccggcca tctggccttc atcggagcgc tgctggcggc cacgctggtc accacgccct
     1741 tcgccattgc cgccgcgctg cgcatctccg gaggctgatc gatggatttc accgccacct
     1801 tcacccgctg gtttcaccgc atggcctcgc cgaggtggtt ctaccggttc gcccggcgct
     1861 ggcagccctg gttcggctgg agcagcctgc tgctgctgct ggtcggggcc gtctggggac
     1921 tcgccttcgc gccgcccgac tacctgcagg gcaacagctt tcgcatcatc tacatccatg
     1981 tgccgg
//
LOCUS       DAORCQ010000175         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_60925_length_1982_cov_4.873897, whole genome shotgun sequence.
ACCESSION   DAORCQ010000175 DAORCQ010000000
VERSION     DAORCQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60925_length_1982_cov_4.873897"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKI23_11580"
     CDS             complement(<1..290)
                     /locus_tag="PKI23_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNN37615.1"
                     /translation="MSANWWQSEFELDGDLAYLNHAGVAPWPRRTRDAVVAFAEENLH
                     RGASGYPRWIEREGALRESLRRLINACCVDEIALLKNTSEVLSVVAHGLSWQP"
     gene            complement(375..>1982)
                     /locus_tag="PKI23_11585"
     CDS             complement(375..>1982)
                     /locus_tag="PKI23_11585"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNN37616.1"
                     /translation="VVVGAGFSGMYMVHKLRDQLGLTVKGYERADNVGGTWYWNRYPG
                     ARCDSESYIYCYSFNKEMLQEWEWSGKYPEQPEILKYLNYAADRLDVRRSFQFNTSVV
                     AARFLDADNLWEIETDRGEKVLARYFITAIGCLSTGQVPKIKGLDSFKGQWYHTGGWP
                     HEGVDFSGKRVGVIGTGSSGVQSIPVIAQQAGHLTVFQRTPQFAVPARHGTVDRAYLE
                     KVKANYDSIWEKARSSYAGYTHDRGTTSALALSDEEREARFEAGWQQGGFQFLSLFSD
                     TSLDRRANDTAAEFIRRKIRQTVKNPETAEKLLPTDHPLGSKRSLIDTDYFETYNRDN
                     VQLVDIRHCPIEEITPNGIKTADGTEYPLDIIVFATGFDAMTGSFFKMNIQGRNGQPL
                     QQKWAEGPKTYLGVSTSGFPNMFTITGPGSPSVLCNMPVCIEQHVEWIADLIQYADQK
                     QIRLIEADVAAENAWVNHVNELASMTMFMLADSWYLGANIPGKPRVFMPYPGGMNTYR
                     LKCEEVAAKQYEGFILQGGDARPPRAA"
BASE COUNT          372 a          618 c          598 g          394 t
ORIGIN      
        1 ggctgccaag acaggccatg cgcgaccacc gagagcacct cggaggtgtt cttcagcagc
       61 gcgatctcat caacgcagca ggcattgatc agccgccgca acgactcgcg cagcgcgccc
      121 tcacgctcga tccagcgggg atagccgctg gcaccacggt gcaggttctc ctcggcaaag
      181 gccaccacgg catcacgggt gcgccttggc cagggggcga caccagcatg gttcaggtag
      241 gccagatcgc catcgagttc gaactcgctc tgccaccagt ttgcactcat gatctgcctc
      301 ctcaaaaaaa tggctccgca tggagtgacc atgcggagcc aggagtggaa cgacggtggg
      361 tgttcgtcga tcgatcaggc cgcacgcggc ggtcgagcgt caccgccttg cagaatgaaa
      421 ccctcatact gtttggccgc cacctcttcg cacttcagcc ggtaggtgtt catgccgccg
      481 ggatagggca tgaacacccg cggttttccg ggaatgttgg cccccagata ccaggagtcg
      541 gccagcatga acatcgtcat cgaggccagc tcgttgacat ggttcaccca ggcgttttcg
      601 gccgcgacat cggcctcgat cagtctgatc tgcttctggt cggcatactg gatcagatcg
      661 gcgatccact ccacatgctg ttcgatgcag accggcatgt tgcacagcac cgacgggctg
      721 cctggaccgg tgatggtgaa catgttggga aagccggagg tcgacacgcc caggtaggtc
      781 ttgggccctt cggcccactt ttgctgcagc ggctgaccat tccggccctg gatgttcatc
      841 ttgaagaacg agccggtcat ggcatcgaag ccggtggcaa agacgatgat gtcgagcgga
      901 tactcggtgc catcggcggt cttgatgccg ttcggcgtga tctcctcgat cggacagtgg
      961 cgaatatcga ccagttgcac attgtcgcgg ttgtaggtct cgaagtagtc agtgtcgatc
     1021 agtgaccgct tggagccgag cggatggtcg gtcggcagca gcttttcggc cgtttccggg
     1081 ttcttcacgg tctgccggat cttgcgtcgg atgaactcgg cggcggtgtc gttggcacgc
     1141 cgatcgaggc tggtgtcgct gaagagcgac aggaactgga agccgccctg ctgccatccc
     1201 gcctcaaacc gtgcctcgcg ctcttcgtcg gaaagcgcca gggccgaggt cgtaccccgg
     1261 tcatgggtat agcccgcgta ggagctgcgc gccttttccc agatcgaatc gtagttggcc
     1321 ttgaccttct ccagataggc acggtcgacc gtaccatggc gcgccggcac ggcgaactgt
     1381 ggcgtacgct ggaagacggt cagatgaccg gcctgctggg caatcaccgg aatcgactgc
     1441 accccgctcg aaccggtgcc gatcacgccc acccgtttgc cgctgaaatc gaccccttca
     1501 tgcggccagc cgccggtgtg gtaccactgc cccttgaagc tgtcgagccc cttgatcttg
     1561 ggcacctgcc cggtggagag acagccgatg gcggtgatga agtaacgcgc cagcaccttc
     1621 tcgccgcggt cggtctcgat ctcccacagg ttgtcggcat cgagaaatct tgcagcgacg
     1681 acactggtgt tgaactgaaa gctgcgccgg acatcgagcc tgtcggcggc ataattcaaa
     1741 tacttgagaa tttccggctg ctcgggatat ttgccgctcc actcccactc ctgcagcatc
     1801 tccttgttga aggagtagca gtagatgtag ctctccgagt cgcagcgtgc accgggatag
     1861 cggttccagt accaggtgcc accgacattg tcggcccgct catagccctt gacggtcaac
     1921 cccagctgat cgcgcagctt gtgcaccatg tacatgccgg aaaatccggc acccaccacc
     1981 ac
//
LOCUS       DAORCQ010000176         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_62812_length_1951_cov_4.699367, whole genome shotgun sequence.
ACCESSION   DAORCQ010000176 DAORCQ010000000
VERSION     DAORCQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62812_length_1951_cov_4.699367"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /gene="leuB"
                     /locus_tag="PKI23_11590"
     CDS             complement(<1..978)
                     /gene="leuB"
                     /locus_tag="PKI23_11590"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNN37617.1"
                     /translation="MGKQLLILPGDGIGPEIVAAALDVLDAAKERFGLDIHCSEGLLG
                     GAAIDATGDPLPEQTLAQARAADAILMGSVGGPKWDALETGKRPEKGLLRLRSGLDLF
                     ANLRPALLFPQLAAASSLKPELVAGLDILIVRELTGGLYFGEPRGVRTLANGERQGFN
                     TLVYSESEIRRIGRVAFELARKRHGRLCSIDKANVLEVTVLWREVMESLAADYPDVQL
                     SHMYVDNAAMQLVRAPKQFDVLVTENMFGDILSDTAAMLTGSIGMLPSASLNANGQGL
                     YEPVHGSAPDIAGRNLANPLATILSLAMLMRYSLDLPQVADAIEAAVSSV"
     gene            complement(1001..1654)
                     /gene="leuD"
                     /locus_tag="PKI23_11595"
     CDS             complement(1001..1654)
                     /gene="leuD"
                     /locus_tag="PKI23_11595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNN37618.1"
                     /translation="MEKFIQLEGLVAPLDRANVDTDQIIPKQFLKAIGRTGFGPNLFD
                     GWRYLDEGEPGMDNSRRPLNPDFVLNQPRYQGASVLLARSNFGCGSSREHAPWALLDQ
                     GFRALIAPSYADIFYNNCFKNGILPITLPEQQVDTLFAEVEAAPGYRLLIDLPEQCVI
                     TPAGVRFGFEIEPFRKQCLLDGLDEISLTLEAAEQIRAYEQRRRQQQPWLFGTLADD"
     gene            complement(1668..>1951)
                     /locus_tag="PKI23_11600"
     CDS             complement(1668..>1951)
                     /locus_tag="PKI23_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNN37619.1"
                     /translation="VPGSGPVKRAAEAEGLDRIFKAAGFEWREPGCSMCLAMNADRLG
                     AGEHCASTSNRNFEGRQGHGGRTHLVSPAMAAAAAIQGRFTDVRHWQYR"
BASE COUNT          352 a          655 c          626 g          318 t
ORIGIN      
        1 cacgctggag acggcggcct cgatggcatc ggccacctgc ggcagatcca gcgagtagcg
       61 catcagcatc gccagcgaca ggatggtcgc cagcgggttg gccaggtttc ggccagcgat
      121 gtcgggcgcc gaaccatgca ccggctcata gagcccctgc ccgttggcat tcagcgaggc
      181 cgacggcagc atgccgatcg agccggtcag catcgcggcg gtgtccgaga gaatatcacc
      241 gaacatgttc tcggtgacca gcacgtcgaa ctgcttgggc gcgcgcacca gttgcatcgc
      301 ggcattgtcg acatacatgt ggctcagctg cacatcgggg tagtcggcgg cgaggctctc
      361 catcacttcg cgccacagca ccgtcacctc gagcacgttg gccttgtcga tcgaacagag
      421 ccggccgtgg cgcttgcgcg ccagctcgaa ggccacccgg ccgatgcgcc ggatctccga
      481 ctcgctgtag accagggtgt tgaagccctg acgctcgccg ttggccagcg tgcgcacccc
      541 gcgcggctcg ccgaagtaga ggccgccggt caactcgcgg acgatcagga tgtcgagtcc
      601 ggccaccaac tccggcttca gcgacgaggc ggccgccagt tgcggaaaca gcagcgccgg
      661 acgcaggttg gcgaaaaggt cgagcccgga gcgcagccgc aacagcccct tctcgggccg
      721 tttgccggtc tccagtgcat cccacttcgg gccgccgacc gatcccatca aaatggcatc
      781 ggccgcacgt gcctgcgcca gcgtctgctc cggcagcgga tcgccggtgg catcgatggc
      841 ggcgccgccc agcagccctt cggagcagtg gatgtcgagc ccgaaacgct ccttcgcggc
      901 atcgagcaca tcaagtgccg cggcgacgat ctccggaccg atgccatcgc cgggcagaat
      961 cagcagttgt ttacccattg gaacctcacg aagggtgaat tcagtcatct gccagcgtgc
     1021 cgaacagcca gggctgctgc tggcggcggc gctgctcgta ggcgcgaatc tgctcggcgg
     1081 cctccagtgt caggctgatc tcatcgagcc catcgagcag gcactgcttg cgaaatggct
     1141 cgatctcgaa accgaagcgg acacccgccg gggtgatgac gcactgctcg ggcagatcga
     1201 tcagcaaccg atagcccgga gcggcttcga cctcggcgaa cagggtatcg acctgctgct
     1261 cgggcagggt gatcggcaag atgccattct tgaagcagtt gttgtagaaa atgtcggcgt
     1321 agctcggcgc gatcagggca cgaaacccct gatcgagcag cgcccagggg gcatgctcgc
     1381 ggctcgatcc gcagccgaag ttgctgcgtg ccagcagcac cgacgccccc tgataacgcg
     1441 gctggttcag cacgaaatcg gggttgagcg gccgccggct gttgtccatg ccgggctcgc
     1501 cttcgtcgag gtagcgccag ccatcgaaca ggttggggcc aaagccggtg cggccgatcg
     1561 ccttcagaaa ctgcttcgga atgatctggt cggtatcgac attggcccga tcgagcggtg
     1621 ccaccaaacc ttcgagctgg atgaacttct ccattggagc ctcctgttta acggtattgc
     1681 cagtgacgca cgtcggtgaa gcggccctga atcgccgccg ccgccgccat cgccgggctg
     1741 accagatggg tgcgtccacc gtgcccctga cgcccctcga agttgcggtt cgaggtcgag
     1801 gcacagtgtt cgccggcccc cagccggtcg gcattcatcg ccagacacat cgaacagccc
     1861 ggttcgcgcc actcgaagcc ggcggccttg aagatccggt cgagcccctc cgcctcggca
     1921 gcacgcttga ccggacccga accgggcacc a
//
LOCUS       DAORCQ010000177         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_71909_length_1817_cov_6.037457, whole genome shotgun sequence.
ACCESSION   DAORCQ010000177 DAORCQ010000000
VERSION     DAORCQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71909_length_1817_cov_6.037457"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1174
                     /locus_tag="PKI23_11605"
     CDS             89..1174
                     /locus_tag="PKI23_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37620.1"
                     /translation="MAPTQPHPFHPLGVTFTTLALLSSPPLLADENWIGARPDAHAPI
                     GVMADHAHKNGEWMFGYRYMRSEQSGLIDGTDSVSVQQTYGSIASGGYGYHDAPLEMR
                     MEMHMFELMYALDDTMTLMAMLPYQKMQMSMELHQHGSNPPVRYGMESEGIGDLELST
                     LLRLTPVAAPEQIHLNLGISLPTGSITEEDAMPHGPGGSFIDMRMEYPMQLGSGTYDL
                     LPGITYFAQHGDCSWGGQGIATLRLGRNSADYSLGDRIDLTGWFAYRFSPSWSGSVRL
                     NGASWGDIDGRDSRLSPTMSPASNPEAQAGERVEAAAGLNYRVPTGNLAGHRFGIELA
                     KPLWERLDGPQLESDWSLVAGWQYAFK"
     gene            1242..>1817
                     /locus_tag="PKI23_11610"
     CDS             1242..>1817
                     /locus_tag="PKI23_11610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNN37621.1"
                     /translation="MSSDPLREAQILRCWNSNAGAWTNAVRQGEIASRRHATDQAIVT
                     AILDHSPSRLLDIGCGEGWLARELVKHSIEVIGIDATAALIEAARQAGGADFRLLDYA
                     QIAAGALDLKVDMAVANFSLLGHESVEKLFGNMPALLNAEGLFIVQTLHPLRVDAETT
                     YRDGWRSGSWQGFSSAFTDPSDWYFRTLQSWT"
BASE COUNT          353 a          597 c          555 g          312 t
ORIGIN      
        1 ccaccgctgc gacaatctgt cgcattctat tgccccgcca gcttccctac actgccgcgc
       61 gaatcgacac tgctccagga gtttggccat ggccccgaca cagcctcacc ctttccaccc
      121 tctcggcgtc acattcacca cgctggccct gctgagcagc ccgccgctgc tggccgacga
      181 gaactggatc ggcgcgcggc ccgatgccca tgcgccgatc ggcgtgatgg ccgaccatgc
      241 ccacaaaaac ggtgaatgga tgttcggcta ccgctacatg cgcagcgaac agagcggatt
      301 gatcgacggc accgattcgg tcagcgtgca gcagacctac ggcagcatcg ccagcggcgg
      361 ctacggttac cacgatgccc cgctggagat gcgcatggag atgcacatgt tcgagctgat
      421 gtacgcgctc gacgacacca tgacgctgat ggcgatgctg ccgtaccaga agatgcagat
      481 gagcatggaa ctgcatcagc atggcagcaa tccgccggtg cgctatggca tggagtccga
      541 agggatcggt gatctcgaac tctcgacgct gctgcgcctg acgccggtgg cggcccctga
      601 gcagatccat ctgaacctcg gcatcagcct gccgaccggc agcatcaccg aggaagatgc
      661 catgccgcat ggccccggcg gcagcttcat cgacatgcgc atggagtacc cgatgcagct
      721 gggttcgggc acctatgacc tgctgcccgg catcacctac ttcgcgcagc atggcgactg
      781 ctcgtggggc ggccagggca tcgccacgct acggcttgga cgaaacagtg cggactactc
      841 gctcggtgac cggatcgatc tgaccggctg gttcgcctac cgtttctcgc ccagctggag
      901 cggttcggtg cggctgaacg gtgccagttg gggcgacatc gacggtcggg actccagact
      961 ctcacccacc atgtcaccgg cctccaaccc cgaggcacag gccggcgagc gggtggaagc
     1021 cgccgcgggt ctcaactacc gggttccgac cggcaatctg gccggccacc gcttcggcat
     1081 cgaactggcc aaaccgctgt gggagcggct cgatggcccg caactggaga gcgactggag
     1141 cctggtcgct ggctggcaat acgcgttcaa atgaccatgg gcagcagcgg aacgtcatga
     1201 ttcaatgaca ttcccagcac aaaccccgcc aggaggcctc catgtccagt gatccactgc
     1261 gagaagcgca gatcctgcgt tgctggaaca gcaatgcagg cgcctggacc aatgcagtgc
     1321 ggcagggcga gatcgccagc cgtcgtcatg ccaccgatca ggccatcgtc actgccattc
     1381 tcgaccactc accaagccgc ctgctcgaca tcggctgtgg cgaaggctgg ctggcgcgtg
     1441 aactggtcaa gcattcgatc gaggtgatcg gcatcgatgc caccgcagcg ctgatcgaag
     1501 ccgcccgcca ggccggcggc gccgattttc gtctgctcga ctacgcacag atcgcggccg
     1561 gagcactcga tttgaaggtc gacatggcgg tggcgaattt ttcgctgttg ggccatgaat
     1621 cggtcgagaa gctgttcggg aacatgcccg ccttgttgaa tgccgaagga ctgttcatcg
     1681 tgcagaccct gcatccgctg agggtcgacg cagagaccac ctaccgggac ggctggcgaa
     1741 gcggctcatg gcaaggcttc agcagtgcct tcaccgaccc atccgactgg tacttcagaa
     1801 ccctgcagag ctggaca
//
LOCUS       DAORCQ010000178         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_72306_length_1812_cov_15.627205, whole genome shotgun
            sequence.
ACCESSION   DAORCQ010000178 DAORCQ010000000
VERSION     DAORCQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72306_length_1812_cov_15.627205"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1812
                     /locus_tag="PKI23_11615"
     CDS             <1..>1812
                     /locus_tag="PKI23_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNN37622.1"
                     /translation="YTFSETDLSIIRQRRGPANRLGFAVQLCYLRFPGVILGVDEPPF
                     PPLLKLVADQLKVSVESWDEYGQREQTRREHLVELQTVFGFQPFTMGHYRQAVQLLTE
                     MALQTDKGIVLASTLIEHLRQQSVILPALNAVERASAEAITRANRRIYDALAEPLSDA
                     HRRRLDDLLKRRDNGKTTWLAWLRQSPVKPNSRHMLEHIERLKAWQALDLPSGIERSV
                     HQNRLLKIAREGGQMTPADLAKFEAQRRYATLVALAIEGMATVTDEIIDLHDRILGKL
                     FNAAKNKHQQQFQASGKAINAKVRLFGRIGQALIEAKQAGRDPFAAIEAVMSWDAFAE
                     SVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVLDAIEVLRG
                     MNTDNARKVPADAPTDFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQG
                     SRQFKDFEDYLVPPAKFASLKQSSALPLAVATDCDQYLHDRLTLLEAQLATVNRMAAA
                     NDLPNAIITESGLKITPLDAAVPDTAQALIDQTAMILPHVKITELLLEVDEWTGFTRH
                     FTHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCP"
BASE COUNT          355 a          630 c          539 g          288 t
ORIGIN      
        1 actacacgtt cagcgaaacc gacctctcca tcatccggca gcggcgcggc ccggccaacc
       61 ggctgggctt cgccgtgcag ctctgttacc tgcgctttcc tggtgtcatc ctgggcgtcg
      121 atgagccgcc gtttccgccc ttgttgaaac tggtcgccga ccagctcaag gtcagcgtcg
      181 aaagctggga cgaatacggg cagcgggagc agacccggcg cgagcacctg gtcgaactgc
      241 aaacggtgtt cggcttccag ccctttacca tgggccacta ccggcaggcc gtccagttgc
      301 tgaccgagat ggccttgcag accgacaagg gcatcgtgct ggccagcacc ttgatcgagc
      361 acctgcggca gcagtcggtc attctgcctg ccctcaacgc cgtcgagcgg gcgagcgccg
      421 aagcaatcac ccgcgccaac cggcgcatct acgatgcctt ggccgaaccg ctgtcggacg
      481 cgcatcgccg ccgcctcgac gatctgctca agcgtcggga caacggcaaa acgacctggc
      541 tggcctggct gcgccaatcg cccgtcaaac cgaattcgcg gcacatgctg gaacacatcg
      601 aacgcctcaa agcgtggcag gcgctcgacc tgccttctgg catcgagcgg tcggtgcacc
      661 agaaccgcct gctcaagatc gcccgtgagg gtggccagat gacgcccgcc gacttggcca
      721 agttcgaggc gcagcgacgc tatgccaccc tggtggcgct tgccatcgag ggcatggcca
      781 ccgtcaccga cgaaatcatc gacctgcacg accgcatcct gggcaagctg ttcaacgccg
      841 ccaagaacaa gcatcagcag cagttccagg catccggcaa ggcgatcaac gccaaggtgc
      901 ggctgttcgg gcgcatcggc caggcgctga tcgaggccaa gcaagcgggc cgcgatccgt
      961 tcgccgccat cgaggccgtc atgtcctggg atgccttcgc cgaaagcgtc accgaggcgc
     1021 agaagctcgc tcagcccgat gacttcgatt tcctgcaccg catcggcgaa agttacgcca
     1081 ccttgcgccg ctacgcgccg gaattccttg ccgtgctcaa gctgcgggcc gcacccgccg
     1141 ccaagaacgt gctcgatgcc atcgaggtac tgcgtggcat gaacaccgac aacgcccgca
     1201 aagtgcctgc cgatgccccg accgacttca tcaagccgcg ctggcagaag ctggtgatga
     1261 ctgacgcggg tatcgaccgg cgctactacg agctgtgcgc gctgtccgag ctgaagaact
     1321 cgctgcgctc gggcgacatc tgggtgcagg gatcgcgcca gttcaaggac ttcgaggact
     1381 acctggtgcc gcccgcgaag ttcgccagcc tcaagcagtc cagcgcattg ccgctggccg
     1441 tggccaccga ctgcgaccag tacctgcacg accgcctgac gctgcttgaa gcgcagcttg
     1501 ccacggtcaa ccgcatggca gcggccaacg acctgccgaa tgcgatcatc accgagtcgg
     1561 gcctgaagat cacgccgctg gatgcggcgg tacccgacac cgcgcaggcg ctgatcgacc
     1621 agacagccat gatcctgcca cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt
     1681 ggacgggctt cacccgccac ttcacgcacc tgaaatcggg cgatctggcc aaggacaaaa
     1741 atctgctgtt gaccaccatc ctggccgacg cgatcaacct gggcctgacc aagatggccg
     1801 agtcctgccc cg
//
LOCUS       DAORCQ010000179         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_72309_length_1812_cov_5.273762, whole genome shotgun sequence.
ACCESSION   DAORCQ010000179 DAORCQ010000000
VERSION     DAORCQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72309_length_1812_cov_5.273762"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..687)
                     /locus_tag="PKI23_11620"
     CDS             complement(61..687)
                     /locus_tag="PKI23_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNN37623.1"
                     /translation="MKLYNSDLSPFAARCRMQLRAKHLPVELAAPPADRSAVNPVGKI
                     PALHVDGRIIPESEVICEYLEDRFPEPPLRPASLEDRALVRTLSRIADLYVLAPLFEI
                     FGQINPATRDTALVTKKLAELLKGLDQLEQFISGERYAVDDRLTLADCTLAPALFFVV
                     ALAPRFGEADILAARPRLKSYWQNIGQDPIAAGVLAEMGRAMQQRMGK"
     gene            complement(722..1507)
                     /locus_tag="PKI23_11625"
     CDS             complement(722..1507)
                     /locus_tag="PKI23_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37624.1"
                     /translation="MNQKTAVNPVAAATVLICRDSPQGIEVFMVVRHHQIDFASGALV
                     FPGGKTDKGDYDSRLRARCTAADGLDDEALAYYVSSIREAFEECGVLLARPRGSAALI
                     DGVRLKALEPERGALVNGKIGFFDFLEREDLQLAIDELHPFAHWITPEMVPKRFDTRF
                     YLAKAPSDHVAAHDGSESVDSVWITPQQALEGAQQGTYTVIFPTKLNIMKLGLSQTVD
                     EAIARAGREPIVTVQPWIEQRAEGNFLTIPKAAGYPISEEKVG"
BASE COUNT          326 a          582 c          552 g          352 t
ORIGIN      
        1 gcagatcgcg ccagcaccag ccgccatgcc aggcgccatg caccagcagc agattggcca
       61 ctacttgccc atgcgctgct gcatcgcccg ccccatctcg gccagcacgc cggcggcgat
      121 ggggtcctgg ccgatgttct gccagtaact tttgagtctg ggccgcgcgg ccagaatgtc
      181 ggcttcgcca aaccgcggtg cgagcgccac cacgaagaag agcgccggcg ccagcgtgca
      241 gtcggccagc gtcagccggt cgtcgaccgc atagcgttca cccgagatga actgttcgag
      301 ctgatcgagc cccttcagca gctcagccag ctttttcgtc accagcgccg tgtcgcgggt
      361 ggccggattg atctggccga agatctcgaa cagtggcgcc agtacataga ggtcggcaat
      421 gcgcgacagc gtgcgcacca gcgcccgatc ctccagactg gccggccgca acggcggttc
      481 aggaaaacgg tcttcgaggt attcgcagat cacctcggac tcgggaatga tgcgcccatc
      541 gacatgcagc gccggaatct tgcccaccgg attgacggca ctgcgatccg ccggcggcgc
      601 agccagctcg accggcaggt gcttggcacg cagctgcatt cggcagcgcg cggcgaaggg
      661 ggagagatcg ctgttgtaga gtttcatgcc actgctctgc tgtgatgttc gattcagtcg
      721 ttcagccgac cttctcttcg gagatcgggt aaccggccgc cttgggaatg gtcagaaagt
      781 tgccttcggc acgctgttcg atccatggct gtaccgtgac gatcggttcg cgcccggcgc
      841 gggcaatggc ctcgtcgacc gtctggctca ggcccagctt catgatgttg agcttggtcg
      901 gaaagatcac cgtgtaggtg ccctgctgtg ccccctccag cgcctgctgc ggggtgatcc
      961 agaccgagtc gaccgactcg ctgccatcgt gggcggcaac gtgatcactc ggcgccttgg
     1021 ccagatagaa gcgggtatcg aaccgcttgg gcaccatctc cggcgtgatc cagtgggcaa
     1081 aggggtgcaa ttcatcgatc gccagttgca gatcttcgcg ttcgagaaaa tcgaagaagc
     1141 caatctttcc gttgaccagt gcaccacgct ccggctccag cgccttcagc cgaacgccat
     1201 cgatcaaggc tgccgagcca cgcggccggg ccagcaacac gccacactcc tcaaacgcct
     1261 cgcgaatcga cgagacatag taggccagcg cctcatcatc gagtccgtcg gccgcggtgc
     1321 agcgggcgcg cagccgggaa tcgtaatcgc ccttgtcggt cttgccaccc ggaaagacca
     1381 gtgcgccgga ggcgaaatcg atctggtggt gacgcaccac catgaacacc tcgatgccct
     1441 gcgggctgtc gcggcagatc agcacggtgg ctgcggcaac cgggttcacg gctgttttct
     1501 gattcatctt cgcgctcctt gtggtgctgc cgcccgtgat cagccgctcg agcggatcac
     1561 cgcctcgctc cggacggtgc ggcaggcttt ttcattattt tcggatggtc tagattgacg
     1621 gcgcacgcta gcagcttgcc cggtggtttg gcaatcgcag cgctcagggt actgcgcgca
     1681 cggaccattg catgcaacat ggttgatctt ccgcatgctt ttgcggatac tccattgaat
     1741 tcaacaggct gtaccttgac cgcccccggc tgtcaccgcc acttcgatga tttgttcatg
     1801 gagtttcaga aa
//
LOCUS       DAORCQ010000180         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_73969_length_1790_cov_5.381556, whole genome shotgun sequence.
ACCESSION   DAORCQ010000180 DAORCQ010000000
VERSION     DAORCQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73969_length_1790_cov_5.381556"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1731)
                     /gene="xrtD"
                     /locus_tag="PKI23_11630"
     CDS             complement(181..1731)
                     /gene="xrtD"
                     /locus_tag="PKI23_11630"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HNN37625.1"
                     /translation="MQWNFLLLFLLLIALVAGALAFDSGLAWLGKEWGREEYSHGYLL
                     VLVALFMVLQKRSELHLASPADRSTWLGWAALLAGLLALLLGRLTTLHIIEHYGLLMV
                     IFGLSGALLGWRGVRLIWPGLLLLVFAIPLPPFLYNNLSGQLQLISSQIGVAVISLFD
                     IPVYLEGNVIDLGVYQLQVVEACSGLRYLFPLMSFGYIAAYLYKGPWWHRLLVLLSTI
                     PITVLMNSFRIGVIGVTVDRWGIEMAEGFLHDFEGWVIFMSCTAILLLEMWLLNWSRR
                     NHEPFREVFGVDFGGNASPGAFAGRPLPTAFVASVVALVIAMGGMFALGERQELIPTR
                     QEFQQFPLTLDGWKGKSQRIEDRYLDSLKLTDYHMADYLSAQGDWVNFYVAYYDSQRS
                     GASAHSPRSCIPGDGWRISNLETQPVPGIEIAGHPLTINRVQIQKGDNRQLVYYWFQQ
                     RGRIINNEYLVKWYLFWDALTRNRSDGALVRFTTPLRPGEQWAEGDARIRELIRQVGP
                     KIVAYVPE"
BASE COUNT          411 a          590 c          511 g          278 t
ORIGIN      
        1 caaatgtcgg tgcccaccga tccgccaatt gcgcggtcca ggtggtcagc agatcaggac
       61 tgaacatgcg caaccatcac cttgctgctg aaaaaatcca cgccgcgctc cggttgatcg
      121 tcctgaagaa gccgacgaat ccggcgcatt tcaagcccat ccccaccggg cacagtgtct
      181 ctattcgggc acataggcca cgatcttcgg tcccacctgc cggatcagct cgcggatgcg
      241 ggcgtcaccc tctgcccact gctcaccggg ccgcagtggc gtggtgaagc gcaccagagc
      301 accatcggag cggttgcggg tcagcgcatc ccagaacaga taccacttca ccagatactc
      361 attgttgatg atgcggccgc gctgctggaa ccagtaatag accagttgtc gattgtcacc
      421 cttctggatc tgcacccggt tgatggtgag cggatggccg gcaatctcga tgcccggcac
      481 cggttgcgtt tcgagattgg agatgcgcca gccatcgccg ggaatgcagg agcgcggcga
      541 atgcgccgag gcaccgctgc gctgcgagtc gtagtaggcc acatagaaat tgacccagtc
      601 gccctgtgcc gacagatagt cggccatgtg atagtcggtc agcttgagtg agtcgagata
      661 gcggtcctcg atgcgctgac tcttgccctt ccagccatcc agcgtcagcg gaaactgctg
      721 gaactcctgc cgcgtcggta tcagctcctg acgctcgccc aacgcgaaca tcccgcccat
      781 cgcgatgacc agcgccacca cgctggcgac gaaggcggtc ggcagcggtc gaccggcaaa
      841 ggcgccggga gaggcattgc cgccgaaatc gacaccgaac acctcacgga atggctcgtg
      901 gttgcgccgg ctccagttga gcagccacat ctccagcagc aggatggcgg tgcagctcat
      961 gaagatgacc cagccctcga agtcatgcag aaagccttcg gccatctcga tgccccagcg
     1021 atcgacggtg acgccgatca ccccgatgcg aaagctgttc atcaacaccg tgatcggaat
     1081 ggtcgacaac agcaccagca accggtgcca ccatggcccc ttgtagaggt aggcggcaat
     1141 gtagccaaag ctcatcagcg gaaagagata acgcaggccg ctgcacgcct ccaccacctg
     1201 caactgatac accccgaggt cgatcacatt gccttcgaga tagaccggaa tgtcgaacag
     1261 cgagatcacc gccaccccga tctgcgacga gatcagctgc aactgacccg agaggttgtt
     1321 gtacaaaaac ggcggcagcg gaatggcgaa caccagcagc aacagcccgg gccagatcag
     1381 ccgcacgccg cgccagccga gcagcgcgcc actcagaccg aagatcacca tcagcagacc
     1441 atagtgctcg atgatgtgca gcgtggtcag ccgccccagc agcagcgcca gcaggccggc
     1501 cagcaacgca gcccatccaa gccaggtgga gcggtcggcc ggactggcca gatgcagctc
     1561 gctgcgtttc tgcagcacca taaagagggc caccagcacc agcagatagc cgtggctgta
     1621 ctcctcacgc ccccactcct tgcccagcca ggccagaccg ctgtcgaagg ccagggcacc
     1681 ggccaccagc gcgatcaaca gaagaaacag cagcagaaaa ttccactgca ccgggtgcct
     1741 gccaagcaat gacccattca tggctgttca aacctcgaaa aaagatgggg
//
LOCUS       DAORCQ010000181         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_74606_length_1782_cov_5.835553, whole genome shotgun sequence.
ACCESSION   DAORCQ010000181 DAORCQ010000000
VERSION     DAORCQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74606_length_1782_cov_5.835553"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKI23_11635"
     CDS             <1..612
                     /locus_tag="PKI23_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HNN37626.1"
                     /translation="PPDYLQGNSFRIIYIHVPAAILAQSGYLLMALAGGIGLIWRIKL
                     AEMVAKSCAPIGASFTFLALVTGAIWGKPTWGTWWVWDARITSMLILLFLYCGVIALQ
                     SAIDNATTAARAAAILALVGVVNIPIIKYSVEWWHSLHQPATFRLTEKPAMPMEMWLP
                     LLVMVVGYYLFFAAALLQRTRCELVERERKSRWVKELVENDEF"
     gene            602..775
                     /gene="ccmD"
                     /locus_tag="PKI23_11640"
     CDS             602..775
                     /gene="ccmD"
                     /locus_tag="PKI23_11640"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNN37627.1"
                     /translation="MSFDSFDALLRMGGHGGYVWSAYAIALATFAYNWVAPLHRRRQL
                     RRRLQQLARRAEP"
     gene            772..1209
                     /gene="ccmE"
                     /locus_tag="PKI23_11645"
     CDS             772..1209
                     /gene="ccmE"
                     /locus_tag="PKI23_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNN37628.1"
                     /translation="MNPIRRQRLFLVIFILLGVGVAVALTLTALQENINLFYSPKQIL
                     AGEAPTDRPIRAGGMVVAGSVKRDPDSLKVAFEVTDYSGTIAVDYEGILPDLFKEEQG
                     IVALGRWAPSGRFVATEVLAKHDENYMPPEVAAALKANPPKPK"
     gene            1226..>1782
                     /gene="ccsA"
                     /locus_tag="PKI23_11650"
     CDS             1226..>1782
                     /gene="ccsA"
                     /locus_tag="PKI23_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNN37629.1"
                     /translation="MIVELGHFALILAFMLALALVVIPLGGVLSGRRAWMALARPLAS
                     GQFVLLLLSAAALEHALLTDDFSVAYVASNSNSQLPAIYKFSAFWGAHEGSLLLWLLL
                     LAGWSFAVARFSRRLPLDMVARVLAVMGAVATGFHLFLLTTSNPFARLLPIPPAEGRD
                     LNPLLQDPGLVIHPPMLYMGYVGFSV"
BASE COUNT          272 a          569 c          596 g          345 t
ORIGIN      
        1 ccgcccgact acctgcaggg caacagcttt cgcatcatct acatccatgt gccggcggcg
       61 attctggcgc agtcgggcta tctgctgatg gcgctggccg gcggcatcgg gctgatctgg
      121 cgcatcaagc tggccgagat ggtggccaag tcgtgcgcgc cgatcggcgc ctccttcacc
      181 tttctggcac tggtgaccgg ggcgatctgg ggcaagccga cctggggcac ctggtgggtg
      241 tgggacgccc gcatcacctc gatgctgatc ctgctgtttc tctactgcgg ggtgatcgcg
      301 ctgcaatcgg cgatcgacaa cgcgaccacc gccgcccgcg ccgccgcgat tctggcgctg
      361 gtgggggtgg tcaacatccc gatcatcaag tactcggtgg agtggtggca ctcgctgcac
      421 cagccggcca ccttcaggct gaccgaaaag ccggcgatgc cgatggagat gtggctgccg
      481 ctgctggtga tggtggtggg gtactatctg ttctttgctg ccgcgctgct gcaacgcacc
      541 cgctgcgagc tggtcgaacg ggaacgcaag tcacgctggg tcaaggagct ggtggagaac
      601 gatgagtttt gacagtttcg acgcgctgct gcggatgggc ggccacggcg gttatgtctg
      661 gagcgcctac gccatcgcgc tggccacctt tgcctacaac tgggtcgcgc cgctgcatcg
      721 ccgccggcag ttgcgacgcc gtctgcaaca actggcacgc agggccgaac catgaatcca
      781 atccgccggc aacggctgtt tctggtgatc ttcattctgc tgggggtcgg cgtggcggtg
      841 gcactgacgc tgacggcgct gcaggagaac atcaacctgt tctacagccc caagcagatt
      901 ctggccggcg aggcgccgac cgaccggccg atccgcgccg gtggcatggt ggtggccggc
      961 agcgtcaagc gtgaccccga cagcctgaag gtggccttcg aggtgaccga ctacagcggc
     1021 accatcgcgg tcgactacga gggcatcctg cccgatctgt tcaaggagga gcagggcatc
     1081 gtcgccctcg gtcgctgggc gccttccggc cgcttcgtcg ccaccgaggt gctggccaag
     1141 catgatgaga actacatgcc gcccgaggtg gccgcagcgc tgaaggccaa tccaccgaaa
     1201 ccgaagtgaa caacggaccg agcagatgat cgtcgaactg ggccactttg cgctgattct
     1261 cgccttcatg ctggcgctgg cgctggtggt gattccgctg ggcggcgtgc tgagcggccg
     1321 ccgcgcctgg atggcactgg cacgcccact ggccagcggg cagttcgtgc tgctgctgtt
     1381 gagcgcggcc gcgctggaac acgcactgct gaccgatgac ttttcggtcg cctatgtggc
     1441 cagcaactcc aacagccagt tgcctgccat ttacaaattc agcgccttct ggggcgcgca
     1501 cgaagggtcg ctgctgctgt ggctgctgct gctggccggc tggagttttg ccgtggcccg
     1561 cttcagccgc cggctgccgc tcgacatggt ggcgcgggtg ctggcggtga tgggggcagt
     1621 cgcgaccggc tttcatctgt tcctgctgac cacctccaac ccctttgccc ggctgctgcc
     1681 gattccacca gccgaagggc gcgacctcaa tccgctgctg caggatccgg gtctggtgat
     1741 ccatccgccg atgctctaca tgggctatgt cggcttctcg gt
//
LOCUS       DAORCQ010000182         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_77078_length_1752_cov_4.050088, whole genome shotgun sequence.
ACCESSION   DAORCQ010000182 DAORCQ010000000
VERSION     DAORCQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77078_length_1752_cov_4.050088"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKI23_11655"
     CDS             <1..475
                     /locus_tag="PKI23_11655"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNN37630.1"
                     /translation="YVGVVDPNGGINILWPLFGISNQILAAIALCVATGILVKSGRVR
                     YAWVTGLPLTWLTLITSSAAWEKIMSADPRIGFFAAANDMAGKLAAGLLSPERAAVAP
                     QLILNQRIDGYLTLFFALLLWVILLDMLRVVWRVLSQRRVPPSTETVYQARTGAG"
     gene            482..682
                     /locus_tag="PKI23_11660"
     CDS             482..682
                     /locus_tag="PKI23_11660"
                     /inference="COORDINATES: protein motif:HMM:NF016234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HNN37631.1"
                     /translation="MQADDRKTEGRIARCWAAVRQWSGDDAYERYLAHQRLAHPDAAP
                     LDRRAFHRHYQQQRFSGVNRCC"
     gene            complement(782..1303)
                     /locus_tag="PKI23_11665"
     CDS             complement(782..1303)
                     /locus_tag="PKI23_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNN37632.1"
                     /translation="MPAQGEYLGMPVYIDDLDSESRAFFKHCASHQLHLQACNECNLL
                     RYPPTTACPWCACLESTWKPVEGRGTLYSYGEVHHAIQPAFRNHTPYMIMLVELDTQK
                     GVPSEHEALRIMGNLATADGDLAPPEMVRQVGIGSRLRIVYKDIGEELSQPLWMLDET
                     AEQPAQPWRYAIE"
     gene            complement(1320..>1752)
                     /locus_tag="PKI23_11670"
     CDS             complement(1320..>1752)
                     /locus_tag="PKI23_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37633.1"
                     /translation="YYAKEILFPNAGVTPEEIDVTGSYDAFTFTTLLQLEDYGFCKKG
                     EGGDYVSNGTMRLGGRRPNNTSGGHLCEGYTHGIAMVIENVRQLRHDADDSCPIGPDG
                     KRQHSYDYREGGCRQVRKVDLTANLGWATPGQGSALVMTNI"
BASE COUNT          284 a          530 c          576 g          362 t
ORIGIN      
        1 ctacgtgggc gtggtcgatc ccaacggcgg catcaacatc ctctggccgc tgtttggcat
       61 ctcaaaccag attctggccg ccatcgccct ctgcgtcgcc accggcattc tggtcaaatc
      121 gggacgcgtt cgttatgcgt gggtgactgg gttgccgctc acctggctga cgctgatcac
      181 cagcagcgcc gcctgggaga agatcatgag tgccgatccg cggatcggct ttttcgctgc
      241 ggccaatgac atggccggca agctggcggc cggcctgctg tcaccggaac gggcggcggt
      301 ggcaccgcaa ctgattctga atcagcgcat cgacggctat ctgaccctct tctttgccct
      361 gttgctctgg gtcattctgc tcgacatgct gcgggtggtc tggcgggtgc tcagtcagcg
      421 cagggtgccg ccgtcgaccg agaccgttta ccaggcgcgc actggcgccg gatgagtcgc
      481 gatgcaggca gatgacagaa aaacggaggg gcggatcgcc cgctgctggg cggcagtgcg
      541 gcagtggagt ggcgatgatg cctacgagcg ctatctggcg caccagcggc tggcgcatcc
      601 cgatgcagcg ccgctcgatc ggcgtgcctt tcatcgtcac tatcagcagc agcgcttcag
      661 cggcgtcaac cgctgttgtt gaaaccgtct ttgtgatctc gcgcgctgcg cagttcgagg
      721 ctcggtcagg gcaggggaaa agcaggcggt cgggcgccgg gacgcccgac cgcttcacgc
      781 ctcactcgat cgcatagcgc cagggctgtg cgggttgctc ggcggtttca tccagcatcc
      841 acagcggctg gctcagctct tcgccaatgt ccttgtagac gatgcgcagt cgcgagccaa
      901 tgccgacctg acgaaccatt tcgggtggcg ccagatcacc atcagctgtc gccagattgc
      961 ccatgatccg cagtgcctca tgttcgctcg gcaccccttt ttgggtgtcg agttccacca
     1021 gcatgatcat gtagggggtg tggttgcgaa aggccggctg aatggcgtgg tgcacctcgc
     1081 cataggagta gagggtgcct cgcccttcga ccggcttcca ggtcgattcg agacaggcgc
     1141 accaggggca ggcggtggtg ggcgggtagc gcagcaggtt gcactcattg caggcttgca
     1201 gatgaagctg atggctggcg cagtgtttga aaaaggcacg gctctcactg tcgagatcat
     1261 cgatgtagac cggcattccg agatattcgc cttgtgctgg catgtcgttg ttctccctgt
     1321 cagatgttcg tcatcaccag tgccgacccc tgcccgggtg tcgcccagcc caggttggcg
     1381 gtcagatcga ccttgcggac ctggcgacag cccccttcac ggtagtcgta gctgtgctgg
     1441 cgcttgccgt cgggcccgat cgggcagctg tcgtcggcat cgtgacgcag ctgccggaca
     1501 ttctcgatca ccatcgcgat gccatgggtg taaccctcgc agaggtgacc gccgctggtg
     1561 ttgttcggac ggcgcccgcc gaggcgcatg gtgccgttgg agacatagtc gcccccctcg
     1621 cccttcttgc agaagccata gtcctcgagt tgcagcagcg tggtgaaggt gaaggcgtcg
     1681 taggagccgg tgacgtcgat ctcttccggc gtgacgccgg cattggggaa gagaatctcc
     1741 ttggcgtaat ag
//
LOCUS       DAORCQ010000183         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_81032_length_1707_cov_4.291162, whole genome shotgun sequence.
ACCESSION   DAORCQ010000183 DAORCQ010000000
VERSION     DAORCQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81032_length_1707_cov_4.291162"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /gene="ilvD"
                     /locus_tag="PKI23_11675"
     CDS             <1..1280
                     /gene="ilvD"
                     /locus_tag="PKI23_11675"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNN37634.1"
                     /translation="GSCSGMFTANSMNCLTEALGLALPGNGSLLATHADRRELFLQAA
                     RRIVELTRRHYEEDDPAVLPRAISNFRAFENAMTLDIAMGGSTNTVLHLLAAAQEGEI
                     DFTMRDIDRLSRKVPNLCKVAPATASYHMEDVHRAGGVMAILGELDRAGLLHTDTTTV
                     HAPTLGDALVRWDIVRSGDAAVREFYRAGPAGIPTQTAFSQSTRYPSLDLDRSAGCIR
                     NLENAYSRDGGLAVLFGNIALNGCIVKTAGVDDSLLRFAGPARIFESQESAVAAILDD
                     QLKPGDVVVIRYEGPRGGPGMQEMLYPTSYIKSKGLGKACALLTDGRFSGGTSGLSIG
                     HVSPEAAEGGAIALIEEGDLIEIDIPQRRIQLAVSDAELARRREAMVARGSAAWSPGE
                     VRSRKVSPALRAYALLTTSADRGAVRDLTLLQR"
     gene            complement(1197..>1707)
                     /locus_tag="PKI23_11680"
     CDS             complement(1197..>1707)
                     /locus_tag="PKI23_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNN37635.1"
                     /translation="YWTHRLMHSRALFRHLHVVHHRSRRPTPFAAYSFNIGEALITAL
                     FFLLVPMLIPLHGLVMLIYLYLLMFRNAFGHSNVELFPAGFIDGPLGWNITNTHHDLH
                     HRDLRHNFALYFTWWDRWMGTEHPAYRETFAAATCGPQSASALQQSEVAHRAAVGTGG
                     EQGVGAQRG"
BASE COUNT          317 a          577 c          550 g          263 t
ORIGIN      
        1 gcggctcctg ctcgggcatg ttcaccgcca actcgatgaa ctgcctgacc gaggcactcg
       61 ggctggcgct gcccggcaac ggcagcctgc tcgccaccca tgccgatcgg cgcgaactgt
      121 tcttgcaggc cgcacggcgt atcgtcgaac tgacgcgccg ccactatgag gaagatgacc
      181 cggccgttct gccacgcgcc atctccaact ttcgcgcctt cgagaacgcc atgacgctcg
      241 acatcgccat gggtggctcg accaacacgg tgctgcacct gctggccgcc gcccaggagg
      301 gcgagatcga cttcacgatg cgtgacatcg atcggctctc gcgcaaggtg ccgaacctct
      361 gcaaggtggc accggccacc gccagctacc acatggagga tgtccaccgc gccggcggcg
      421 tgatggcgat tctgggcgaa ctcgaccgcg ccggactgct gcacaccgac accaccacgg
      481 tgcatgcccc cacgctgggt gacgcgctgg tccgctggga catcgtccgc agcggcgatg
      541 ccgcggtcag ggagttctac cgggccggtc cggccggcat ccccacccag accgccttca
      601 gtcagtcgac ccgctatcca agcctcgacc tcgaccgcag tgccggctgc atccgcaatc
      661 tcgaaaatgc ctacagccgc gatggcggcc tggcggtgct gttcggcaac atcgccctca
      721 atggctgcat cgtcaagacc gccggcgtcg acgacagcct gctgcgcttt gccggcccgg
      781 cccgcatctt cgagagccag gagagcgccg tggccgccat tctggatgac cagctcaaac
      841 cgggcgacgt ggtggtgatc cgctacgaag ggccacgcgg cgggcccggc atgcaggaga
      901 tgctctaccc gaccagctac atcaaatcga aagggctcgg caaggcttgc gcgctgctga
      961 ccgatggccg cttctccggc ggcacttcgg ggctgtcgat cggccatgtc tcgcccgaag
     1021 ccgccgaagg gggcgccatc gcactgatcg aagagggtga cctgatcgag atcgacattc
     1081 cgcaacggcg catccagctg gcggtcagcg acgctgaact ggcgcgccgg cgcgaggcga
     1141 tggtcgcccg cggcagcgcg gcatggagcc ccggcgaagt gcgcagccgc aaggtctcac
     1201 ccgcgctgcg cgcctacgcc ctgctcacca ccagtgccga ccgcggcgcg gtgcgcgacc
     1261 tcactctgtt gcagcgctga ggccgactgt ggaccacagg tggctgcggc gaaagtctcc
     1321 cgataggcag gatgctcggt tcccatccag cggtcccacc aggtgaagta gagcgcgaag
     1381 ttgtgccgca gatcgcggtg gtgcagatcg tggtgggtgt tggtgatgtt ccagcccaac
     1441 ggaccatcga tgaatccagc cggaaacagc tcgacattgc tgtgcccgaa tgcattgcgg
     1501 aacatcagca gatagagata gatcagcatc accagaccat gcagtggtat cagcatcggc
     1561 accagcaaaa agaacaacgc cgtgatcagc gcctcgccaa tgttgaagga gtaggcagca
     1621 aagggcgtgg ggcgacgcga gcggtggtgc accacatgca ggtggcggaa cagcgcccgg
     1681 ctgtgcatca gccggtgggt ccagtaa
//
LOCUS       DAORCQ010000184         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_85185_length_1662_cov_4.930305, whole genome shotgun sequence.
ACCESSION   DAORCQ010000184 DAORCQ010000000
VERSION     DAORCQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85185_length_1662_cov_4.930305"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..623)
                     /locus_tag="PKI23_11685"
     CDS             complement(132..623)
                     /locus_tag="PKI23_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNN37636.1"
                     /translation="MAPEILQSTSTYAASLAALAAIFSGWCAFLSYRLARKIHDDLKT
                     DERIVAGTSIHPDLHEHMHSDCVIQCTLFNKSKRKAYVNAVSVYDKNGTKIDVTWSDA
                     IDRLGNVQNPCQLIGLVDTCPLFIREKNGKSISYARIKISHSFSDTPMEVIFDLTAGW
                     ISE"
     gene            complement(644..1030)
                     /gene="gcvH"
                     /locus_tag="PKI23_11690"
     CDS             complement(644..1030)
                     /gene="gcvH"
                     /locus_tag="PKI23_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNN37637.1"
                     /translation="MSQIPDHLKYLSSHEWANLEPGGLVSVGITDHAQEQLGDVVFVE
                     LPDVGIKVSAGQEVAVVESVKAASDIYAPVSGTVVEVNDALGTAPELVNQSPYEQGWF
                     FRIEPSDLTELDGLLDADGYADVVEG"
     gene            complement(1062..>1662)
                     /locus_tag="PKI23_11695"
     CDS             complement(1062..>1662)
                     /locus_tag="PKI23_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNN37638.1"
                     /translation="DLPPFMAMQHGDRFIARTGYTGEDGFEILLPGDSAGALWEQLRS
                     AGITPAGLGARDTLRIEAGLNLYGAEMDESVNPLIANLGWTIAWEPPTRDFIGRAALT
                     RLREQGVQQCQLGLLLEGRGVLRAGQRIFTATGEGIVTSGTFSPTLGHSIAIARLPLG
                     SSGRVEAEIRGRRLPAQVVKLPFVRHGQPLHPPVTPTSA"
BASE COUNT          300 a          484 c          530 g          348 t
ORIGIN      
        1 cagcttcgtg tgcgatgagc gtcgcaatgc gctccggatc ggttgtcttg aaatgttctg
       61 ggaggtacat gcgaactgaa ttccagcatc gttattggtc cgctctttgg agcacgcgct
      121 cgcagcgcgt ttcattcgct aatccaacct gccgtgagat caaagattac ctccatcggt
      181 gtatccgaga aggaatgaga gattttgatt cgagcgtagc ttattgattt tccgtttttc
      241 tctctgatga aaagcgggca tgtatccacg agtccgatca gttggcacgg gttctgtacg
      301 ttacctagtc gatcaatcgc gtcagaccac gtgacgtcga tcttcgtgcc attcttgtcg
      361 taaaccgata ccgcgtttac gtatgccttt ctcttcgatt tgttaaatag agtgcattga
      421 atgacgcagt cagaatgcat gtgttcgtgt aaatcaggat ggatcgaggt tccagcgaca
      481 atccgttcgt ctgtttttaa atcgtcgtgg atcttgcgcg ccagccgata gctaagaaag
      541 gcgcaccagc ccgagaaaat cgccgccaat gccgccagcg acgcggcgta agtggatgta
      601 gattggagta tctccggagc catagcggac ctcgagcctc caactaccct tcgaccacat
      661 cggcataacc atcggcatcg agcaggccgt cgagttcggt caggtcgctc ggttcgatgc
      721 ggaagaacca gccctgctcg tagggtgact gattgaccag ctccggtgcc gtgccgagtg
      781 catcgttgac ctcgaccacg gtgccggaga ccggggcgta gatgtccgag gccgccttga
      841 ccgactccac caccgccacc tcctgtccgg cgctcacctt gatgccgaca tcgggcagtt
      901 cgacaaagac cacatcgccc agttgctcct gggcatggtc ggtgatgccg acgctgacca
      961 ggccgcccgg ttcgaggttc gcccactcat ggctgctgag gtatttgagg tgatcgggta
     1021 tctggctcat ggcagtctct cttgttatga agttgaaagg gtcaggcaga ggtgggggtg
     1081 accggtggat ggagcggttg gccatggcgc acgaacggca gcttcaccac ctgggccggc
     1141 agccgtctgc cgcggatctc ggcctcgacc cggccgctgc tgccgagcgg cagccgggcg
     1201 atggcgatcg aatggcccag cgtcggcgaa aaggtgccgc tggtgacgat gccttcgccg
     1261 gtggcggtga agatgcgctg tccggcacgc agcacgccgc ggccttcgag cagcaggcca
     1321 agctggcact gctgcacccc ctgttcgcgc agtcgggtca gcgcggcgcg gccgatgaag
     1381 tcacgcgtgg gcggctccca ggcgatggtc cagccgaggt tggcgatcag cgggttgacg
     1441 ctctcatcca tctcggcgcc atagaggttg aggccggcct cgatgcgcag cgtgtcgcgg
     1501 gcacccaggc cggccggcgt gatgccggcg ctgcgcaact gctcccacag tgcgccggcg
     1561 ctgtcgccgg gcagcaggat ctcgaagccg tcctcgccgg tgtagccggt gcgggcgatg
     1621 aagcggtcgc cgtgctgcat ggccatgaag gggggcaggt cg
//
LOCUS       DAORCQ010000185         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadales bacterium isolate AMR_MDS_0902
            NODE_103050_length_1509_cov_5.096974, whole genome shotgun
            sequence.
ACCESSION   DAORCQ010000185 DAORCQ010000000
VERSION     DAORCQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852245
            Sequence Read Archive: SRR3501888
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadales bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadales bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 16:02:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,341
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,291
            CDSs (with protein)               :: 2,291
            Genes (RNA)                       :: 36
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Pseudomonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103050_length_1509_cov_5.096974"
                     /isolate="AMR_MDS_0902"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1891229"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKI23_11700"
     CDS             <1..859
                     /locus_tag="PKI23_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNN37639.1"
                     /translation="DGASLARLIHDEGANLLLGVPTLWLDLLNHLRAQEQRLDGVERV
                     VIGGAAAPYSMIKEFDERHGVFVVHAWGMTETSPIGTLNSPTPQMRRLPAEARYRQQM
                     KQGRPPFGVEIRIVNDAGEALPEDGKTFGRLLVRGPWVANGYFRSEERTAFVNGWFDT
                     GDIATIDPSGTMQIVDRAKDVIKSGGEWISSIELENLAIGHPAVKEACVVGVPHPKWD
                     ERPLLLLVLQPQAQLTEAEMLSHLQDKVAKWWLPDRVIIVDTLPHGSTGKLQKNVLRE
                     QYQQLLLKG"
     gene            906..1358
                     /locus_tag="PKI23_11705"
     CDS             906..1358
                     /locus_tag="PKI23_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNN37640.1"
                     /translation="MSSTLEMLQSHIGKVMGPSAWFTVDQARINAFADATGDHQFIHV
                     DPEQAKHSPFGGTIAHGYLSLSLLPFLQKDISIRPEGVKMGVNYGLDKVRFLTPVKSG
                     GRVRLTNRLLSVEEKRPGQFLMKSEVTMEVEGADKPALIAETLGLVYV"
BASE COUNT          302 a          518 c          450 g          239 t
ORIGIN      
        1 cgatggtgca tcgctcgccc gcctgattca cgacgaaggc gccaacctgc tgctcggcgt
       61 ccccaccctc tggctcgacc tgctcaacca tttgcgcgcg caggagcagc gcctcgacgg
      121 cgtcgagcgg gtggtgatcg gcggcgccgc ggccccctac tcgatgatca aagagttcga
      181 cgaaaggcat ggcgtcttcg tcgtccacgc ttggggcatg accgagacca gcccgatcgg
      241 caccctcaac agcccgacgc cgcagatgcg ccggctgccg gccgaagcgc gctatcggca
      301 gcagatgaaa cagggccgcc cgccgttcgg ggtcgagatc aggatcgtca acgacgccgg
      361 cgaggccctg cccgaagatg gcaagacctt cggccgcctg ctggtgcgtg ggccctgggt
      421 ggccaatggc tacttcagaa gcgaagagcg caccgccttc gtcaacggct ggttcgacac
      481 cggcgacatc gccaccatcg acccctccgg caccatgcag atcgtcgatc gggccaagga
      541 tgtcatcaaa tcgggcggcg agtggatcag ctcgatcgaa ctggagaacc tcgccatcgg
      601 ccacccggca gtgaaggagg cttgcgtggt cggcgtgccc catcccaaat gggatgagcg
      661 accgctgctg ctgctggtgc tgcagccgca agcccagctc accgaggccg aaatgctcag
      721 ccacctgcag gacaaagtgg caaaatggtg gctgcccgat cgggtgatca tcgtcgatac
      781 cctgccacac ggcagcactg gcaagttgca gaaaaatgtt ctgcgcgagc agtatcaaca
      841 gctcctgctc aaaggctaga tcgcgtggcc cgccacgccc gaaaaacgac aacgaggtga
      901 tgaaaatgtc cagtacgctc gaaatgctgc aatcccacat cggcaaggtg atggggccct
      961 ccgcatggtt caccgtcgac caggctcgca tcaacgcctt tgccgatgcc accggcgacc
     1021 accagttcat tcatgtcgat cccgaacagg ccaaacactc gcccttcggc ggtaccatcg
     1081 cccatggcta cctctcgctg tcactgctgc ccttcctgca aaaggacatc tcgatccgcc
     1141 cggaaggcgt gaagatgggc gtcaactacg gcctcgacaa ggtgcgcttt ctcaccccgg
     1201 tcaaaagcgg cggccgggtg cggctcacca acaggctgct gtcggtcgaa gagaaacgtc
     1261 cggggcagtt tctgatgaag tcggaggtga ccatggaggt cgaaggcgcc gacaagccgg
     1321 cgctgatcgc cgagaccctc ggactggtct acgtctgaga tgccgcgggg tgcggccgac
     1381 gccgcactcc gcaccctgcc gcgctggccg agccaatcct gactacactc aaagcaaccg
     1441 tctcagcttc atccatcgtc cgcatccgcc gtcaggtgcc cgccgatgca caccgccacc
     1501 gccccgacc
//
